LOCUS       DAPBCS010000001       224704 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPBCS010000001 DAPBCS010000000
VERSION     DAPBCS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 224704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.001"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="RD521_000001"
     CDS             complement(99..1229)
                     /locus_tag="RD521_000001"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDT7769772.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="RD521_000002"
     CDS             complement(1878..3080)
                     /locus_tag="RD521_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7769773.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="RD521_000003"
     CDS             complement(3140..3343)
                     /locus_tag="RD521_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDT7769774.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="RD521_000004"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="RD521_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="RD521_000005"
     CDS             4880..5692
                     /locus_tag="RD521_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7769775.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="RD521_000006"
     CDS             complement(6716..6913)
                     /locus_tag="RD521_000006"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDT7769776.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="RD521_000007"
     CDS             complement(7059..7937)
                     /locus_tag="RD521_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7769777.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="RD521_000008"
     CDS             complement(7976..9094)
                     /locus_tag="RD521_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDT7769778.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="RD521_000009"
     CDS             complement(9091..11190)
                     /locus_tag="RD521_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDT7769779.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="RD521_000010"
     CDS             complement(11241..12062)
                     /locus_tag="RD521_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7769780.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="RD521_000011"
     CDS             complement(12059..12868)
                     /locus_tag="RD521_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7769781.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="RD521_000012"
     CDS             complement(12889..13383)
                     /locus_tag="RD521_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7769782.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="RD521_000013"
     CDS             complement(13395..13883)
                     /locus_tag="RD521_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7769783.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="RD521_000014"
     CDS             complement(13960..16656)
                     /locus_tag="RD521_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7769784.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="RD521_000015"
     CDS             complement(17084..18157)
                     /locus_tag="RD521_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7769785.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="RD521_000016"
     CDS             complement(18154..18981)
                     /locus_tag="RD521_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7769786.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="RD521_000017"
     CDS             complement(18982..19788)
                     /locus_tag="RD521_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7769787.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="RD521_000018"
     CDS             complement(19800..20885)
                     /locus_tag="RD521_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7769788.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="RD521_000019"
     CDS             complement(20902..21444)
                     /locus_tag="RD521_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7769789.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="RD521_000020"
     CDS             21702..22691
                     /locus_tag="RD521_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDT7769790.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="RD521_000021"
     CDS             22688..23425
                     /locus_tag="RD521_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDT7769791.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="RD521_000022"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="RD521_000022"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDT7769792.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="RD521_000023"
     CDS             23989..24621
                     /locus_tag="RD521_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7769793.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="RD521_000024"
     CDS             24706..25353
                     /locus_tag="RD521_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7769794.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="RD521_000025"
     CDS             25358..26341
                     /locus_tag="RD521_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDT7769795.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26744..28258)
                     /locus_tag="RD521_000026"
     CDS             complement(26744..28258)
                     /locus_tag="RD521_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDT7769796.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28396..29202
                     /locus_tag="RD521_000027"
     CDS             28396..29202
                     /locus_tag="RD521_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769797.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29377..29946)
                     /locus_tag="RD521_000028"
     CDS             complement(29377..29946)
                     /locus_tag="RD521_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7769798.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30029..32089)
                     /locus_tag="RD521_000029"
     CDS             complement(30029..32089)
                     /locus_tag="RD521_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDT7769799.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32366..33844)
                     /locus_tag="RD521_000030"
     CDS             complement(32366..33844)
                     /locus_tag="RD521_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7769800.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34142..35077)
                     /gene="murB"
                     /locus_tag="RD521_000031"
     CDS             complement(34142..35077)
                     /gene="murB"
                     /locus_tag="RD521_000031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDT7769801.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35150..36070)
                     /locus_tag="RD521_000032"
     CDS             complement(35150..36070)
                     /locus_tag="RD521_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7769802.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36183..36935
                     /gene="xth"
                     /locus_tag="RD521_000033"
     CDS             36183..36935
                     /gene="xth"
                     /locus_tag="RD521_000033"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDT7769803.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37799..38332
                     /locus_tag="RD521_000034"
     CDS             37799..38332
                     /locus_tag="RD521_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDT7769804.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(38822..39346)
                     /locus_tag="RD521_000035"
     CDS             complement(38822..39346)
                     /locus_tag="RD521_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7769805.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(39339..39812)
                     /gene="tsaE"
                     /locus_tag="RD521_000036"
     CDS             complement(39339..39812)
                     /gene="tsaE"
                     /locus_tag="RD521_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDT7769806.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(39935..40915)
                     /gene="pta"
                     /locus_tag="RD521_000037"
     CDS             complement(39935..40915)
                     /gene="pta"
                     /locus_tag="RD521_000037"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDT7769807.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(41068..41748)
                     /locus_tag="RD521_000038"
     CDS             complement(41068..41748)
                     /locus_tag="RD521_000038"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDT7769808.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            41874..42743
                     /locus_tag="RD521_000039"
     CDS             41874..42743
                     /locus_tag="RD521_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7769809.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            42769..43284
                     /locus_tag="RD521_000040"
     CDS             42769..43284
                     /locus_tag="RD521_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769810.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(43331..43924)
                     /locus_tag="RD521_000041"
     CDS             complement(43331..43924)
                     /locus_tag="RD521_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7769811.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(43949..44128)
                     /locus_tag="RD521_000042"
     CDS             complement(43949..44128)
                     /locus_tag="RD521_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769812.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(44205..44714)
                     /locus_tag="RD521_000043"
     CDS             complement(44205..44714)
                     /locus_tag="RD521_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769813.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(44810..45190)
                     /locus_tag="RD521_000044"
     CDS             complement(44810..45190)
                     /locus_tag="RD521_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7769814.1"
                     /translation="MNLTTIHPIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            45520..46269
                     /locus_tag="RD521_000045"
     CDS             45520..46269
                     /locus_tag="RD521_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDT7769815.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKLTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(46453..47730)
                     /locus_tag="RD521_000046"
     CDS             complement(46453..47730)
                     /locus_tag="RD521_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769816.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(47809..48453)
                     /gene="pcp"
                     /locus_tag="RD521_000047"
     CDS             complement(47809..48453)
                     /gene="pcp"
                     /locus_tag="RD521_000047"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDT7769817.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(48652..49596)
                     /locus_tag="RD521_000048"
     CDS             complement(48652..49596)
                     /locus_tag="RD521_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDT7769818.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(49598..50284)
                     /locus_tag="RD521_000049"
     CDS             complement(49598..50284)
                     /locus_tag="RD521_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDT7769819.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            50757..51077
                     /locus_tag="RD521_000050"
     CDS             50757..51077
                     /locus_tag="RD521_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDT7769820.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            51238..52143
                     /locus_tag="RD521_000051"
     CDS             51238..52143
                     /locus_tag="RD521_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7769821.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            52140..52913
                     /locus_tag="RD521_000052"
     CDS             52140..52913
                     /locus_tag="RD521_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7769822.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(53090..54514)
                     /locus_tag="RD521_000053"
     CDS             complement(53090..54514)
                     /locus_tag="RD521_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7769823.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            54725..55171
                     /gene="nrdI"
                     /locus_tag="RD521_000054"
     CDS             54725..55171
                     /gene="nrdI"
                     /locus_tag="RD521_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDT7769824.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            complement(55241..57223)
                     /locus_tag="RD521_000055"
     CDS             complement(55241..57223)
                     /locus_tag="RD521_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7769825.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(57237..58133)
                     /gene="murQ"
                     /locus_tag="RD521_000056"
     CDS             complement(57237..58133)
                     /gene="murQ"
                     /locus_tag="RD521_000056"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDT7769826.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            complement(58136..59197)
                     /locus_tag="RD521_000057"
     CDS             complement(58136..59197)
                     /locus_tag="RD521_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7769827.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(59409..59975)
                     /locus_tag="RD521_000058"
     CDS             complement(59409..59975)
                     /locus_tag="RD521_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7769828.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            complement(60003..61505)
                     /gene="eat(A)"
                     /locus_tag="RD521_000059"
     CDS             complement(60003..61505)
                     /gene="eat(A)"
                     /locus_tag="RD521_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDT7769829.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            complement(61763..62884)
                     /locus_tag="RD521_000060"
     CDS             complement(61763..62884)
                     /locus_tag="RD521_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7769830.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            63010..63951
                     /locus_tag="RD521_000061"
     CDS             63010..63951
                     /locus_tag="RD521_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDT7769831.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(64094..64990)
                     /locus_tag="RD521_000062"
     CDS             complement(64094..64990)
                     /locus_tag="RD521_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDT7769832.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            65272..66612
                     /locus_tag="RD521_000063"
     CDS             65272..66612
                     /locus_tag="RD521_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7769833.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            66807..67250
                     /locus_tag="RD521_000064"
     CDS             66807..67250
                     /locus_tag="RD521_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDT7769834.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(67512..68930)
                     /gene="nhaC"
                     /locus_tag="RD521_000065"
     CDS             complement(67512..68930)
                     /gene="nhaC"
                     /locus_tag="RD521_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDT7769835.1"
                     /translation="MEKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKKYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDILEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPKLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(69334..70557)
                     /locus_tag="RD521_000066"
     CDS             complement(69334..70557)
                     /locus_tag="RD521_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDT7769836.1"
                     /translation="MTTTLSTNQLKDLIQYRLPEYQALALAIHDHPEVSNYEFYSSAA
                     LIKQLEKEGFWVEKEVAGHRTGFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDEIGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDAALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAALSTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSEKGLASIAIAAELLANTALDLLREPKLLEKIKQE
                     HQEIVARKKQEGATF"
     gene            complement(70589..71428)
                     /locus_tag="RD521_000067"
     CDS             complement(70589..71428)
                     /locus_tag="RD521_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDT7769837.1"
                     /translation="MKKIIRFAVITFVAIIWLAGCSQGKAATTEKKETTVKLGIIGED
                     TDVWDDVKDRLKKENIHLEYVKFTDYNQPNAALADGSIDLNSFQHQFFLDNYNEEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENKKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDEHARPYVNIIVARKEDEDNKTYQKIVDAYQTEETAKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(71445..72125)
                     /locus_tag="RD521_000068"
     CDS             complement(71445..72125)
                     /locus_tag="RD521_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7769838.1"
                     /translation="MTEFFKTYLPNVYLIPDEFIEATKQTLYMSFWTAFIGGIIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILVLVFLSQFISN
                     QLIKKVTH"
     gene            complement(72127..73200)
                     /locus_tag="RD521_000069"
     CDS             complement(72127..73200)
                     /locus_tag="RD521_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7769839.1"
                     /translation="MIELRDISVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEAGK
                     IIEKGSSIQIFSHPEEELTKDFIRTATHLDQALETISAHAAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRQKALDYLIKS
                     GVKVTILQKNELDNRIKVIEGGN"
     misc_binding    complement(73259..73497)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(73540..73773)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(73850..74521)
                     /locus_tag="RD521_000070"
     CDS             complement(73850..74521)
                     /locus_tag="RD521_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7769840.1"
                     /translation="MKGAIFDLDGLLVDTERTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVQAAGSPEKVQEVRAKREEYFYQQLTSGAIQLKPYAKEILTDLKERHM
                     KLGIATTTVTKRATDILDHFQLADYFDTFTFGDEVSENKPSPVPYLTALERTGLEALS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYSVEEKEKLNLLAEGKDLRTVIEMLD
                     KKTEK"
     gene            complement(74599..77277)
                     /gene="mgtA"
                     /locus_tag="RD521_000071"
     CDS             complement(74599..77277)
                     /gene="mgtA"
                     /locus_tag="RD521_000071"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDT7769841.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASPNGLS
                     EEQIERSRETYGENAISYGKKTPFIIEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGEYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNDTLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEAAETLDTDTINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVAPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELITGEKISEMNDHELAQVAEEHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGKFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQQVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKIPFIQSNATFAMLVITTLGIIVGSVLPFTGFGAELGLM
                     PLPGNYWLWLFITVVAYLALVTAVKKIYIRRFQELL"
     gene            77937..78374
                     /gene="ndk"
                     /locus_tag="RD521_000072"
     CDS             77937..78374
                     /gene="ndk"
                     /locus_tag="RD521_000072"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDT7769842.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFATMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKGADAVPGTIR
                     GDYALPGTENIIHASDSRDAATKEIARFFPPFDTNKPSEKAAK"
     gene            complement(78396..79262)
                     /locus_tag="RD521_000073"
     CDS             complement(78396..79262)
                     /locus_tag="RD521_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7769843.1"
                     /translation="MYDGELIKKLRVNKKLTQTQLAEGICSKTSLVGIETNSVKKVSF
                     GTLKSFLERMNMTLAEYEWIRNQLDEPKKLKKKRRMLDRIQEDSFDPYKEIANNRRHY
                     KKTEDLYYLVLNLHMSKKTEVSETEFEFLRYECRIIEEYLMQVPEYGQFEVSILADFP
                     WIFSDRFIENNYLKIKKRMRRIAQLNNHEQYLFTFLTNLTLFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKSENHQEFFAVIEYTLGKNQRNSL
                     EKKLLAIAKPSS"
     gene            complement(79496..81574)
                     /locus_tag="RD521_000074"
     CDS             complement(79496..81574)
                     /locus_tag="RD521_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7769844.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVSKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPATLGQTEGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTIL
                     YPVTGTNAEQ"
     gene            82067..83284
                     /locus_tag="RD521_000075"
     CDS             82067..83284
                     /locus_tag="RD521_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7769845.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETREKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEASPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(83743..84366)
                     /locus_tag="RD521_000076"
     CDS             complement(83743..84366)
                     /locus_tag="RD521_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769846.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWEEYKLTANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLVVLLLTHDGDKVARLKNGKVVTAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     ASSQKNESTELSPSDSGSGQTITGNEENQASIITNESSEGQYQTYGQ"
     gene            complement(84522..85919)
                     /locus_tag="RD521_000077"
     CDS             complement(84522..85919)
                     /locus_tag="RD521_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7769847.1"
                     /translation="MKRYQFPKNFWWGSAASGPQTEGRISGDGKGENIFDYWYKNEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HHYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFQLFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKAYHEMDQSGVI
                     GIILNLTPSYPRDESNPEDVKAARIADAFFNRSFLDPAVKGEFPSELIERIKELDLMP
                     EIHPDDLTIIKENTVDLLGINYYQPRRIKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEKGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDRNGFEA"
     gene            complement(85941..86912)
                     /locus_tag="RD521_000078"
     CDS             complement(85941..86912)
                     /locus_tag="RD521_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7769848.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMEKISEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISNRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHAIDGLIVFLSEEEWSGFEMLTKMSIPVVALNCPGGPAGIDTIVVNDR
                     ESVAKVIAHHGGQGHKTIGFIAAAQTDYVARERLAGAKEAVQKDPSIHLEIFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            complement(86919..89219)
                     /locus_tag="RD521_000079"
     CDS             complement(86919..89219)
                     /locus_tag="RD521_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HDT7769849.1"
                     /translation="MYDYPYQNPKLTIRQRVEDLLSRMNIAEKVGQVNQHLYGWECYE
                     KNGDKIELTEKLKEHVKWGGGLGALYGLFRSDPWSKTDYKNGIPAKESWKVANLVQEY
                     VLQHSRWKIPVLLVEECPHGHQGLDGISYPTNIGRGNTFNVALLEQSSRLMAKELAAK
                     GVHLALVSTLDLAKDPRWGRTEECFGEDPILSARFSEAVVRGFQGEIISEKVSFLDQT
                     VEEINKKKDQIGVVLKHCIAQGEALGGHNSGTVTIGRREFSDIYGPLLKSTRNAVGVM
                     AAYNDIDGIPCHINRALFEQTLRKEIGYQGIVMADGVALDRLSDVFTDKKTAAAYALA
                     AGIDLSLWDETYTKIAEAIDNQVVDEKLLDQAVRRVLSVKFLLGLFEQPFANDPKEYW
                     DHLMEESEHLNLETAKESITLLKNDGILPLDQQVKNIAVIGPNAHDVYHLLGDYSAPQ
                     TEDRLKKKIVQEIKTEFPQSKVSYAQGCEVRNQEDQEEKLLEAVTLAQNSDVIIAVLG
                     GSSARNFDMEFLRNGAVSSKGINMDSGENVDVASLSLGGYQEQLIEQLYQLGKPIITI
                     LVQGRPYDLMKIESFTNAIATAWFPGQEGGKAIAQMISGRNNPSGRLSLSYPRNSRQL
                     PVYYYQRAASKQENYYDLSGSPFYPFGHGLSYTTFEYSQMEVKTDIDKVIISIKVKNT
                     GSVSGKTSTLVYVRLIDGVVIQRTKLLKAFRKDELRSQEERLLVFELTSEDFSYMDID
                     DKRHYAKKAVIMVEQQEKEIKCNWQE"
     gene            complement(89221..90546)
                     /locus_tag="RD521_000080"
     CDS             complement(89221..90546)
                     /locus_tag="RD521_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7769850.1"
                     /translation="MKVVDRLSEKMLPIVSKMSSQRHLLAIRDSFIVTMPLVMAASVM
                     VLLNALIFSNATVQRILDLSPLAELASIVNNGTMSILAIIVCYNIGWNLATHYIQTGV
                     IDDPSFSPVHAGILSTAVMFILMSLSSTVTLMDGNTAEATGVFLQSLTSSSGLATAMI
                     AALVSTELFIKLAKMKSFKIRMPEGVPPAVAASFNSLVPEIVVILFFSVVVFTLNHTT
                     GLNVPQLIELAIQTPLKAFVLSVPGILFLQFFSDFLWVFGMHGSSILAPIRQAPLLQS
                     IQENMDAFSVGKEIPNIITEPFTNAFGLIGGGGCILPLVIAILWASKRQEQRSIAKFG
                     LTTCLFNITEPIMFGLPVVMNPIYMIPCAIIPSINLVIAYAATSLGIISKTVAAAPWI
                     TPPVIQSFIATGGDIRAAVLTVILIILDVFLFLPFVLAANKAKLAEGGY"
     gene            90777..91202
                     /locus_tag="RD521_000081"
     CDS             90777..91202
                     /locus_tag="RD521_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769851.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DITNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGADSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKEATVTFTKIGNYRLKDKDGNIYSL"
     gene            complement(91700..92401)
                     /locus_tag="RD521_000082"
     CDS             complement(91700..92401)
                     /locus_tag="RD521_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7769852.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDTFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(92417..92857)
                     /locus_tag="RD521_000083"
     CDS             complement(92417..92857)
                     /locus_tag="RD521_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HDT7769853.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVLAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFEKLPVETYVGEYL"
     gene            complement(92857..93432)
                     /locus_tag="RD521_000084"
     CDS             complement(92857..93432)
                     /locus_tag="RD521_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDT7769854.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(93843..94322)
                     /locus_tag="RD521_000085"
     CDS             complement(93843..94322)
                     /locus_tag="RD521_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HDT7769855.1"
                     /translation="MFLDSGVVLKETELERIEYTDFGLGAIETEGLNLFVYENNERYC
                     GKELVLLPYQTCPEHRHPPRDENEGKKETFRCRKGLVYLYVEGKETKKLSCIIPKGQE
                     RYYTAKKEIILHAGDQYTIEQNIKHWFQAGATGAVITEFSSSSDDPSDSFTNPAIYR"
     gene            complement(94360..94839)
                     /locus_tag="RD521_000086"
                     /pseudo
     CDS             complement(94360..94839)
                     /locus_tag="RD521_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(95011..95712)
                     /locus_tag="RD521_000087"
     CDS             complement(95011..95712)
                     /locus_tag="RD521_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7769856.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQEILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(95820..97262)
                     /locus_tag="RD521_000088"
     CDS             complement(95820..97262)
                     /locus_tag="RD521_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7769857.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCMDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(97276..99141)
                     /locus_tag="RD521_000089"
     CDS             complement(97276..99141)
                     /locus_tag="RD521_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7769858.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(99262..100113)
                     /locus_tag="RD521_000090"
     CDS             complement(99262..100113)
                     /locus_tag="RD521_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7769859.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(100423..101136)
                     /locus_tag="RD521_000091"
     CDS             complement(100423..101136)
                     /locus_tag="RD521_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDT7769860.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(101204..101530)
                     /locus_tag="RD521_000092"
     CDS             complement(101204..101530)
                     /locus_tag="RD521_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDT7769861.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(101653..101880)
                     /locus_tag="RD521_000093"
     CDS             complement(101653..101880)
                     /locus_tag="RD521_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDT7769862.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(102202..103455)
                     /locus_tag="RD521_000094"
     CDS             complement(102202..103455)
                     /locus_tag="RD521_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7769863.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            103516..104256
                     /locus_tag="RD521_000095"
     CDS             103516..104256
                     /locus_tag="RD521_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7769864.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(104342..105100)
                     /locus_tag="RD521_000096"
     CDS             complement(104342..105100)
                     /locus_tag="RD521_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7769865.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(105097..106125)
                     /locus_tag="RD521_000097"
                     /pseudo
     CDS             complement(105097..106125)
                     /locus_tag="RD521_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(106145..109312)
                     /locus_tag="RD521_000098"
     CDS             complement(106145..109312)
                     /locus_tag="RD521_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769866.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSKYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(109536..110420)
                     /locus_tag="RD521_000099"
     CDS             complement(109536..110420)
                     /locus_tag="RD521_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDT7769867.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(110515..110952)
                     /locus_tag="RD521_000100"
     CDS             complement(110515..110952)
                     /locus_tag="RD521_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769868.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(111169..112080)
                     /locus_tag="RD521_000101"
     CDS             complement(111169..112080)
                     /locus_tag="RD521_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDT7769869.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(112537..112965)
                     /locus_tag="RD521_000102"
     CDS             complement(112537..112965)
                     /locus_tag="RD521_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769870.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(112993..113631)
                     /locus_tag="RD521_000103"
     CDS             complement(112993..113631)
                     /locus_tag="RD521_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7769871.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(113635..115617)
                     /locus_tag="RD521_000104"
     CDS             complement(113635..115617)
                     /locus_tag="RD521_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDT7769872.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            115797..116462
                     /locus_tag="RD521_000105"
     CDS             115797..116462
                     /locus_tag="RD521_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7769873.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(117902..122299)
                     /locus_tag="RD521_000106"
     CDS             complement(117902..122299)
                     /locus_tag="RD521_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HDT7769874.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
     gene            complement(122277..123044)
                     /locus_tag="RD521_000107"
     CDS             complement(122277..123044)
                     /locus_tag="RD521_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HDT7769875.1"
                     /translation="MVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGIKEACEE
                     LQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGVELLEDN
                     AQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKRTPDDNP
                     IIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLDLFSFDD
                     EVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            complement(124818..125594)
                     /locus_tag="RD521_000108"
     CDS             complement(124818..125594)
                     /locus_tag="RD521_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769876.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            complement(125795..126121)
                     /locus_tag="RD521_000109"
     CDS             complement(125795..126121)
                     /locus_tag="RD521_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769877.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(126167..126529)
                     /locus_tag="RD521_000110"
     CDS             complement(126167..126529)
                     /locus_tag="RD521_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDT7769878.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWVSMVVSLAFIPVVFGAVAVIMGYLYRETDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(126566..126781)
                     /locus_tag="RD521_000111"
     CDS             complement(126566..126781)
                     /locus_tag="RD521_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7769879.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            126908..127243
                     /locus_tag="RD521_000112"
     CDS             126908..127243
                     /locus_tag="RD521_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7769880.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(127319..129316)
                     /locus_tag="RD521_000113"
     CDS             complement(127319..129316)
                     /locus_tag="RD521_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769881.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(129414..129689)
                     /locus_tag="RD521_000114"
     CDS             complement(129414..129689)
                     /locus_tag="RD521_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7769882.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            129924..130658
                     /locus_tag="RD521_000115"
     CDS             129924..130658
                     /locus_tag="RD521_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7769883.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQVEDMNYPEEAVNEIIDN
                     INKLKQTISSIGKKYTKKKMKGGDTI"
     gene            130697..131839
                     /locus_tag="RD521_000116"
     CDS             130697..131839
                     /locus_tag="RD521_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7769884.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(131934..133325)
                     /gene="sufB"
                     /locus_tag="RD521_000117"
     CDS             complement(131934..133325)
                     /gene="sufB"
                     /locus_tag="RD521_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDT7769885.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(133330..133800)
                     /locus_tag="RD521_000118"
     CDS             complement(133330..133800)
                     /locus_tag="RD521_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDT7769886.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(133787..135022)
                     /locus_tag="RD521_000119"
     CDS             complement(133787..135022)
                     /locus_tag="RD521_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7769887.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAH
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     SFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVINPVQELAELVHENGGVIVVDGAQSAPHMTIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQELGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VIAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFKHGTI"
     gene            complement(135019..136305)
                     /gene="sufD"
                     /locus_tag="RD521_000120"
     CDS             complement(135019..136305)
                     /gene="sufD"
                     /locus_tag="RD521_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDT7769888.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(136318..137091)
                     /gene="sufC"
                     /locus_tag="RD521_000121"
     CDS             complement(136318..137091)
                     /gene="sufC"
                     /locus_tag="RD521_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDT7769889.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(137288..138136)
                     /locus_tag="RD521_000122"
     CDS             complement(137288..138136)
                     /locus_tag="RD521_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDT7769890.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(138160..138846)
                     /locus_tag="RD521_000123"
     CDS             complement(138160..138846)
                     /locus_tag="RD521_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7769891.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(138839..139873)
                     /locus_tag="RD521_000124"
     CDS             complement(138839..139873)
                     /locus_tag="RD521_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7769892.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(139936..140192)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(140268..140606)
                     /locus_tag="RD521_000125"
     CDS             complement(140268..140606)
                     /locus_tag="RD521_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDT7769893.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(140608..140964)
                     /locus_tag="RD521_000126"
     CDS             complement(140608..140964)
                     /locus_tag="RD521_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDT7769894.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(140970..142157)
                     /locus_tag="RD521_000127"
     CDS             complement(140970..142157)
                     /locus_tag="RD521_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7769895.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(142348..143193)
                     /locus_tag="RD521_000128"
     CDS             complement(142348..143193)
                     /locus_tag="RD521_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7769896.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEIANSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            143427..144392
                     /locus_tag="RD521_000129"
     CDS             143427..144392
                     /locus_tag="RD521_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDT7769897.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            144892..145935
                     /locus_tag="RD521_000130"
     CDS             144892..145935
                     /locus_tag="RD521_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDT7769898.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGIKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLFYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(145997..146461)
                     /locus_tag="RD521_000131"
     CDS             complement(145997..146461)
                     /locus_tag="RD521_000131"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDT7769899.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQQILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            146707..147654
                     /locus_tag="RD521_000132"
     CDS             146707..147654
                     /locus_tag="RD521_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7769900.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(147865..149937)
                     /locus_tag="RD521_000133"
     CDS             complement(147865..149937)
                     /locus_tag="RD521_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7769901.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLNLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEEQKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERIPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKMLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(149870..150247)
                     /locus_tag="RD521_000134"
     CDS             complement(149870..150247)
                     /locus_tag="RD521_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDT7769902.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEAEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESGKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(150376..150954)
                     /locus_tag="RD521_000135"
     CDS             complement(150376..150954)
                     /locus_tag="RD521_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7769903.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINDFIATLP"
     gene            151086..152378
                     /locus_tag="RD521_000136"
     CDS             151086..152378
                     /locus_tag="RD521_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7769904.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMSFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSISSGHSPSHRQ"
     gene            complement(152438..153556)
                     /locus_tag="RD521_000137"
     CDS             complement(152438..153556)
                     /locus_tag="RD521_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDT7769905.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SEFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVNPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILER
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(153570..154991)
                     /gene="arcD"
                     /locus_tag="RD521_000138"
     CDS             complement(153570..154991)
                     /gene="arcD"
                     /locus_tag="RD521_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDT7769906.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(155237..156358)
                     /gene="mnmA"
                     /locus_tag="RD521_000139"
     CDS             complement(155237..156358)
                     /gene="mnmA"
                     /locus_tag="RD521_000139"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDT7769907.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGDMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            156762..157361
                     /locus_tag="RD521_000140"
     CDS             156762..157361
                     /locus_tag="RD521_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7769908.1"
                     /translation="MNYFTSDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELITQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(157499..158785)
                     /gene="efmA"
                     /locus_tag="RD521_000141"
     CDS             complement(157499..158785)
                     /gene="efmA"
                     /locus_tag="RD521_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDT7769909.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(159056..159400)
                     /locus_tag="RD521_000142"
     CDS             complement(159056..159400)
                     /locus_tag="RD521_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDT7769910.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(159537..160682)
                     /locus_tag="RD521_000143"
     CDS             complement(159537..160682)
                     /locus_tag="RD521_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7769911.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSSEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(160861..161835)
                     /locus_tag="RD521_000144"
     CDS             complement(160861..161835)
                     /locus_tag="RD521_000144"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDT7769912.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(161915..162790)
                     /locus_tag="RD521_000145"
     CDS             complement(161915..162790)
                     /locus_tag="RD521_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDT7769913.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            162920..164827
                     /locus_tag="RD521_000146"
     CDS             162920..164827
                     /locus_tag="RD521_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDT7769914.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNEQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIQYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(165245..168535)
                     /locus_tag="RD521_000147"
     CDS             complement(165245..168535)
                     /locus_tag="RD521_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDT7769915.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGPDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKDHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERLTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNQATGEVLENAKDLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     ASYETKEILHTVDYRKPNYSTVYVTPEGSDTGNGSRQNPLSLTQALRYAYAGQTIVLA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWVLR
                     GFDVTNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVVIK
                     NSVAYRNGWVPGVEGEGNGNGFKMGDSSLSGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDFHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTVSKDWIESLNTHSQEGQQPVTRYANGSIHVHGLMQIKPEN
                     RQTEKGRVDGLPLSVGATFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVV
                     YVMKQRKK"
     gene            complement(168806..169351)
                     /locus_tag="RD521_000148"
     CDS             complement(168806..169351)
                     /locus_tag="RD521_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7769916.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(169363..170055)
                     /locus_tag="RD521_000149"
     CDS             complement(169363..170055)
                     /locus_tag="RD521_000149"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDT7769917.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(170074..170358)
                     /locus_tag="RD521_000150"
     CDS             complement(170074..170358)
                     /locus_tag="RD521_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769918.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(170371..170934)
                     /locus_tag="RD521_000151"
     CDS             complement(170371..170934)
                     /locus_tag="RD521_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7769919.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            171162..171824
                     /locus_tag="RD521_000152"
     CDS             171162..171824
                     /locus_tag="RD521_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDT7769920.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            171948..173144
                     /locus_tag="RD521_000153"
     CDS             171948..173144
                     /locus_tag="RD521_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDT7769921.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(173245..174621)
                     /locus_tag="RD521_000154"
     CDS             complement(173245..174621)
                     /locus_tag="RD521_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7769922.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVIILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(174658..175524)
                     /locus_tag="RD521_000155"
     CDS             complement(174658..175524)
                     /locus_tag="RD521_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDT7769923.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(175613..176638)
                     /locus_tag="RD521_000156"
     CDS             complement(175613..176638)
                     /locus_tag="RD521_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7769924.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(176656..177330)
                     /locus_tag="RD521_000157"
     CDS             complement(176656..177330)
                     /locus_tag="RD521_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7769925.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(177514..178407)
                     /locus_tag="RD521_000158"
     CDS             complement(177514..178407)
                     /locus_tag="RD521_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7769926.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(178481..180178)
                     /locus_tag="RD521_000159"
     CDS             complement(178481..180178)
                     /locus_tag="RD521_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7769927.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(180171..181484)
                     /locus_tag="RD521_000160"
     CDS             complement(180171..181484)
                     /locus_tag="RD521_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDT7769928.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(181484..185521)
                     /locus_tag="RD521_000161"
     CDS             complement(181484..185521)
                     /locus_tag="RD521_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDT7769929.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(185534..185992)
                     /locus_tag="RD521_000162"
     CDS             complement(185534..185992)
                     /locus_tag="RD521_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7769930.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(186289..186807)
                     /locus_tag="RD521_000163"
     CDS             complement(186289..186807)
                     /locus_tag="RD521_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDT7769931.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(186809..187426)
                     /locus_tag="RD521_000164"
     CDS             complement(186809..187426)
                     /locus_tag="RD521_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDT7769932.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(187423..188277)
                     /locus_tag="RD521_000165"
     CDS             complement(187423..188277)
                     /locus_tag="RD521_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7769933.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(188274..189185)
                     /locus_tag="RD521_000166"
     CDS             complement(188274..189185)
                     /locus_tag="RD521_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7769934.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(189226..190560)
                     /locus_tag="RD521_000167"
     CDS             complement(189226..190560)
                     /locus_tag="RD521_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7769935.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            190768..191598
                     /locus_tag="RD521_000168"
     CDS             190768..191598
                     /locus_tag="RD521_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDT7769936.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(191842..192160)
                     /locus_tag="RD521_000169"
                     /pseudo
     CDS             complement(191842..192160)
                     /locus_tag="RD521_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(192282..192476)
                     /locus_tag="RD521_000170"
     CDS             complement(192282..192476)
                     /locus_tag="RD521_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769937.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(192981..193166)
                     /locus_tag="RD521_000171"
     CDS             complement(192981..193166)
                     /locus_tag="RD521_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769938.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(194016..194357)
                     /locus_tag="RD521_000172"
     CDS             complement(194016..194357)
                     /locus_tag="RD521_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7769939.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(194357..194587)
                     /locus_tag="RD521_000173"
     CDS             complement(194357..194587)
                     /locus_tag="RD521_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7769940.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            194874..195212
                     /locus_tag="RD521_000174"
     CDS             194874..195212
                     /locus_tag="RD521_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769941.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(195417..195656)
                     /locus_tag="RD521_000175"
     CDS             complement(195417..195656)
                     /locus_tag="RD521_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDT7769942.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(195632..195979)
                     /locus_tag="RD521_000176"
     CDS             complement(195632..195979)
                     /locus_tag="RD521_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDT7769943.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(196711..197292)
                     /locus_tag="RD521_000177"
     CDS             complement(196711..197292)
                     /locus_tag="RD521_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7769944.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(197475..198101)
                     /locus_tag="RD521_000178"
     CDS             complement(197475..198101)
                     /locus_tag="RD521_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7769945.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(198123..199454)
                     /locus_tag="RD521_000179"
     CDS             complement(198123..199454)
                     /locus_tag="RD521_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDT7769946.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(199609..200010)
                     /locus_tag="RD521_000180"
     CDS             complement(199609..200010)
                     /locus_tag="RD521_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDT7769947.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(200096..200302)
                     /locus_tag="RD521_000181"
     CDS             complement(200096..200302)
                     /locus_tag="RD521_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769948.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(200397..201899)
                     /locus_tag="RD521_000182"
     CDS             complement(200397..201899)
                     /locus_tag="RD521_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDT7769949.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(201917..202372)
                     /gene="argR"
                     /locus_tag="RD521_000183"
     CDS             complement(201917..202372)
                     /gene="argR"
                     /locus_tag="RD521_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDT7769950.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(202470..203087)
                     /locus_tag="RD521_000184"
     CDS             complement(202470..203087)
                     /locus_tag="RD521_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDT7769951.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            203308..204681
                     /locus_tag="RD521_000185"
     CDS             203308..204681
                     /locus_tag="RD521_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7769952.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            204756..205868
                     /locus_tag="RD521_000186"
     CDS             204756..205868
                     /locus_tag="RD521_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDT7769953.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(205940..207577)
                     /locus_tag="RD521_000187"
     CDS             complement(205940..207577)
                     /locus_tag="RD521_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDT7769954.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(207745..208353)
                     /locus_tag="RD521_000188"
     CDS             complement(207745..208353)
                     /locus_tag="RD521_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7769955.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(208737..209888)
                     /gene="queG"
                     /locus_tag="RD521_000189"
     CDS             complement(208737..209888)
                     /gene="queG"
                     /locus_tag="RD521_000189"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDT7769956.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(209907..210659)
                     /locus_tag="RD521_000190"
     CDS             complement(209907..210659)
                     /locus_tag="RD521_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769957.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(210800..211750)
                     /locus_tag="RD521_000191"
     CDS             complement(210800..211750)
                     /locus_tag="RD521_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDT7769958.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(211751..212590)
                     /locus_tag="RD521_000192"
     CDS             complement(211751..212590)
                     /locus_tag="RD521_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7769959.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(212587..213333)
                     /locus_tag="RD521_000193"
     CDS             complement(212587..213333)
                     /locus_tag="RD521_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7769960.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            213571..214941
                     /locus_tag="RD521_000194"
     CDS             213571..214941
                     /locus_tag="RD521_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7769961.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(214996..215892)
                     /locus_tag="RD521_000195"
     CDS             complement(214996..215892)
                     /locus_tag="RD521_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7769962.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(216006..216176)
                     /locus_tag="RD521_000196"
     CDS             complement(216006..216176)
                     /locus_tag="RD521_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769963.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(216173..216850)
                     /locus_tag="RD521_000197"
     CDS             complement(216173..216850)
                     /locus_tag="RD521_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7769964.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(216941..217846)
                     /locus_tag="RD521_000198"
     CDS             complement(216941..217846)
                     /locus_tag="RD521_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7769965.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            218021..219475
                     /locus_tag="RD521_000199"
     CDS             218021..219475
                     /locus_tag="RD521_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7769966.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            219491..220522
                     /locus_tag="RD521_000200"
     CDS             219491..220522
                     /locus_tag="RD521_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7769967.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            220529..221716
                     /locus_tag="RD521_000201"
     CDS             220529..221716
                     /locus_tag="RD521_000201"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDT7769968.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            221872..223254
                     /locus_tag="RD521_000202"
     CDS             221872..223254
                     /locus_tag="RD521_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7769969.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            223381..223611
                     /locus_tag="RD521_000203"
     CDS             223381..223611
                     /locus_tag="RD521_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769970.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(223811..224479)
                     /locus_tag="RD521_000204"
     CDS             complement(223811..224479)
                     /locus_tag="RD521_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDT7769971.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        65231 a        46974 c        38886 g        73613 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt tacttggctg ttcttattac ttgatgcagg
    26641 gcagcttttt cacaacctct tcactaaaca gtgttcaatc agctgaccct cggtattagc
    26701 tcaaaacgct gatttcacaa cctcttttta tttacgttct atttcattca ttttccgaag
    26761 caaatcgagc tgcttgtatg cccgcttgtc gcccaaaaat aatgatctcc gcaactgaat
    26821 ttccaccgat ccggttgttt ccatgtaagc cacctgtgac ttcaccagca gcatataacc
    26881 ccctaattgg ctggttttct ttatctaata cttcagtgtt cgtattgatt ttcacaccgc
    26941 ccatcgtgta atggattcct ggtgcgattt tgatcgcata ataattcgga gacgataagt
    27001 catgttccat ggctgtggca cggttgaatt gagcatcttt tttctccgca acatccgtat
    27061 tccatccatc aactgttttt gctagtgttt cttttggtaa atcgattttt tctgctaatt
    27121 cttccattgt ctttccagtt gtgacatatc ctttttggtc ataaaattca atagctgtgg
    27181 cacgatcacg cacgccttgg tcaaaaatca aataagctga tttttcaggc aatgctgtaa
    27241 ttgctgcaga tactttatcc cttgtatcca tttcattgac aaatcttttc ccctcttgat
    27301 cgaccaagat tgctccttct ccgcgaacaa cctcaccaat caagaccccc tcatcctgct
    27361 gcacagttgg atgaatttgt attttatcca tatcaacggt ttgtccacct aatttttcaa
    27421 tcatggtgat accatctcca gttgatcctt cttgatttgt cgtaatataa tcagctaaat
    27481 cgggacgata tttttcaata aattctttgc tggcaccaaa accgcctgtg gtaactacga
    27541 ttgcttttcc ggtgacctct tttggttctt ctccttgaac atgtacagtc acttcattga
    27601 ccgtttcccc atttttctta atgtctgtca catctgcgtt gacaaaaata gggatttccc
    27661 gctcatggac gttattcaat aacccctcca ctaagtatcc accaatagcc gagccatcag
    27721 acggacgatg tgtcctcttt tcactcatcc ctccagtgat cgttaagttg tccagtttga
    27781 taccgttttg atctagccaa tcgaccgcat cagcagagtg atcgacaaag tatcgaagca
    27841 tttctttatc attcgttcct ccaccacctt ttagcgtttc ttcatagaag gcatcattcg
    27901 agtctttgat tccttcttct ttttgaaatt ttgtttctga agcattcata ccgcttgatg
    27961 atttagatgt gtttcctccg gcaacaggca tcttttccaa aattactgga ttcaacccag
    28021 catcttttgc ttcgatagct gctgccatcc ctgcaccacc agctccaata atcacgacat
    28081 cataagattc tttcatttct tttggatctg tatatacttg ttcagaagcg cctgatgata
    28141 cttcggcaga ttccttcgta ctagaacttg tctgcgctgt atttttctga tctgcgccgc
    28201 atgcgctcat agcaagaagt gcaacggctg ttaataaacc agcaactaat tttttcattt
    28261 gtatccccct tttctcctta tactatatta agtgattttt ttcacaatat caactctgtt
    28321 tttctttaag tcatatagct ttggtaaaca cacgcttctt agtataatta aggatataca
    28381 tgaaggagtt attttatgaa tcatttgaaa acctttttta cgaacagtac aaaaactgca
    28441 ggtagaatca caaacggaat ctatatttta ttacgtatca tcgtcgttat tatgctagtg
    28501 gatcaggtct ggaaccaaca ctggggaaat acaatgcttt tattactgac attaatttta
    28561 tttgaaatcc cttattttct ggaaaagata ttaaagatac agattccaaa tatgctagaa
    28621 atcattttgt tatcttttat ttttagttca acagttttag gagagttaag tgatttttat
    28681 ggctattttt caatgtggga tacttttcta catgcattca gcggattttt agctgggggt
    28741 gtcggctttt cccttgttta tttgctcaat aaaaatacaa aaaccatgaa cttgactcct
    28801 ttattactag cagttgtttc cttctgtttt tcgatgacag ttggtgttat gtgggagttt
    28861 gtggaatttt cagcagataa attactgcaa ttcgatatgc aaaaagatac gatcatccag
    28921 caaatcaata gtgtggagtt aagtaaagac ggaaacactg tctatcatgt tccagatatc
    28981 actcggacaa tcattgaaag tcaaaatgca gatggtaaaa cagataaaac aatcatccaa
    29041 aatggttact tagacgtggg aatcatggat actatgaagg atttatttgt taattttatt
    29101 ggagcagttg ttttcagtgt atttggttac ctctatagtc gaaacgatta ccaaaaattt
    29161 acgttcatca agaattttat tcctaaaaaa atcaaacgat aaaacatgct aaaaaacaag
    29221 agaaggggca gtgacaatac ttttgtcact gccccttctt ttttgaatgt atggcatgcc
    29281 attagttatc cgatagtatt aactcaaaaa agtaagatat gaggaatgac tatttcgttt
    29341 tttatcccaa tactgaggac ttatttcacc atttcgttat ttatctaatt tacttaaaac
    29401 ctgcataact ttttcataat ctggattagg tgtgccgtct agattttcag caataaaagt
    29461 aaaacccatt tttttataaa gagcaatcgc ctgatggctc catgtctgtg tgtgaagata
    29521 gattggtccg tccgtatgga gggtaggaaa ttcttgcaag actctggcag tcaatgcttt
    29581 tgcgagccct tttccttggt aatctggttt cactgctaac caatgaaaaa ccggatacac
    29641 ttgctctctt gatttttttc gccaagcggt acaggttgct actttttctc ctttttttgt
    29701 ctctacaaaa aacatccttc tttcaagttc ttcaggataa ggagcaaagt tttgttgaaa
    29761 atactcaagt gcttcatctg tcgtttgaaa ttccccaacg gaagtctcaa tctccgccca
    29821 agcagattca tcttcttttt gataaaatcg aaattcaaaa ccttccgcta aaggcgtgat
    29881 tggaacattt tgttgaagtg gtcttgtcat ccagatttct gcgtaaggta acgttttatc
    29941 taacattttt ctatccctcc tcgtctccat tatgccaaat aacatatgac tcagctactt
    30001 atttgactat aacagataga gatataaatc agaaccagtc atctgcgtca aattcatttc
    30061 gattagcgta tacccatgtt cctgccatac ctcgcatgaa atctcctaaa gcagaagaag
    30121 gaatacctcc accaggtgcg ggtgttcccg gactaggaag tggtacttgc ggctgcagat
    30181 gctcatcggt ggaatcaata gttgacaagc gttttttctt cgtttccttc tggtcatctg
    30241 attccttctg atcttccgac tgtttagcaa caaattcttt ttctgattgt tgttttattt
    30301 taggctctaa aatttcattt tccaatccag aaacttgtgg ggccaaatga gcttcttgcc
    30361 ttatcgtttt attagattct gagatttttc tagatattct tgctaactct aggcttcttg
    30421 tttgttttac tttccctgtt ggagaaactg catgggccat ttgttgtttt ttttctgaca
    30481 agctttcttc ttgtcttttt tgttcttttt gatctatctg tttgtttaat ggtaaaacat
    30541 gtaatagtga ctgagcatgg agttcttgga gtgaatacgg gtcatgataa ctatacataa
    30601 tcgtaaaatc acctgtttgt ttggtcaaat aaccatcctc caatggtact tcattgattg
    30661 taaccgatac atcctttaac ggtacgggtt tacctaagga atttgttgct gattggatca
    30721 actcttcttt gtttattttt tcgtttggat gaacaataag atcatgacct tctaatgaac
    30781 gattgtctgg tatataaaca taagtaaagg taatttcccc tcctgattgc ttcctattat
    30841 tcatcagttc tgagtgaagt acttcaagga tctccgacca gtgacttttt ctgtctaatc
    30901 cgtttgcttg catccaatca gaaatagaag tgactttccc attttgttca taagtcgata
    30961 aatctatatt tggcactcga tcagaaaatt cagcaggtag gcgcaaatca ggaatacgat
    31021 ttacactact catatgtgtt tctgcttgaa gttctttatt tgtttgacta tctataaaat
    31081 gagtgatgac agtcgatgaa gcagcagatg cttctacata aactacagct gttgtttctg
    31141 ccacatttga tgttttcgag tctatataag ttaatacaac gggatattct cccggagttg
    31201 tagtatctac tgtcccgttc attttgattt cttctttatt tcctgaagtt ccatcgcttt
    31261 ttgttacata ttcaacaaga tctttagggg cgtaactctc tcctgttctt aagtgaagat
    31321 cttttccttt gatagctgtt tgatcagcac gaacatggat cacagcagat gcagtcattg
    31381 tttttaaagc aattggatca aaataggaca aggaaatagt agatgttcct gtattagtcg
    31441 tatccacgat attcccattg tttgaaatta ctttggacat atctccttgt gttccatctg
    31501 cgttcttcag cctttctacc agatcagata tcgaaacttt tgtccctgcc agaacatcga
    31561 ttttctttct ttcaaactgt gaataatctt tttcatatac ataagaaact tgtgcgcttc
    31621 caccgtcctt tatatctgtc aaagcatgat tcaaaatatc agtgatctgt tttaactgat
    31681 caccttttgt aggatcagtc atatcggaaa agatagaaag cggcacactg cttccggttt
    31741 ccgatgtcca taatgttttg ttaggaatta accagtagtg gtttatcgtt ggaggataac
    31801 tgactttatt ataggaagtc aatgcaggaa gtagctgttt tccatttgca tcagtgaaag
    31861 tatgagtaat agtgataggg gaatcagtgg tctcagaaac agaggtacgt acgaccttga
    31921 tggtttgtgt cgttgcctga acgggtacat gcatatagac acctacagat aaactgatca
    31981 gcatcgtgcg catcataaac ttaacgtagt gtttttcaga acacgaccgt tccttatcta
    32041 aacgacaagc gtgtttattt agttgttttg aacgtctttt tgaacacatt tattcttagt
    32101 cctccctact cttgtctaaa catgaagtag taattttcat aagatgattt cattatataa
    32161 ataaaatcaa aagaagatgg actattagga gagtattttt tttgattttt tattgtttca
    32221 agaaaatcgg aataataact tatgtacttt ttaattttgt taagtaatat atctagttat
    32281 atctagttat atctagttac cttttctttg aaaaaaacat aaatcagcta ataaatatgt
    32341 gagacaaagc tgccatccat aaatactagc tgattttttc agttaaatgt ctattttttt
    32401 ctctgatgat ggtatttcca aaatattccc agtcatctgg agtcggtgga tttttccaaa
    32461 ttatctgttc tttattaaat ttttcaagta aacaatcaga taaataaata ttcgtacgag
    32521 aagtgattcg actctcaatc acaatattgt aatttttaaa tcctttcact tgagaaataa
    32581 tgtaatcggt ataagcagat ccttgcgaaa aatcaataca gacatgaatc ggtgcttcaa
    32641 aatatgatac cggacaaata ccgaccaata aaaagagata atcataaaat aagcgaatca
    32701 tgaaacgttc atcttttccc aagaaatagc gtttattctc ttgtatgacc ttttggattg
    32761 ctgtactgaa tattggatac gtctcgaaga aaaactgtag ctgcttttta gacgtaaagc
    32821 tagtcatctc aaagtcgaat atccaatttt ttcgattgac atgcatcata cccatacgaa
    32881 gttcttttcg tatatatagt ctttcttctt cagagatttc aatcgaaaat ggtaattgat
    32941 caatcaattc cactgacatt ttttgactaa tcatgtggat atcatgaaaa tgagcttccg
    33001 caagtccaaa tgcgccatct aattcaaatt cttcttccgt gatcaaaaac aacatgatat
    33061 aagaaatctc atcaatgatc tgctctttag ggatatgaag aaaagaaata tgtgataaat
    33121 ccttgatgat cttcattcct tctgagctct taaagtattc atctgaaaag aaacgttttc
    33181 cgattggttg attcccatag gccctcaaca aactaatagt ggttagcaaa ttgaatttga
    33241 ttcgttttgt tgcagacagt tttaaattga acagatattc aaaatattgc acgatactct
    33301 ttgcttcatt catgacgctt tctctaaatg gcatagaaaa accattaaag accccgaaat
    33361 ataccccaaa taatacgttc cgtatgttta tttcttcccc aactaatatg tttttcttta
    33421 actttatttg atgctgttca agaaaagaga ctaattctct tctttttaca taggctcttg
    33481 atatgcttaa aaaatgattc tctgcaaatt tttcgatcgt tacttttgaa tccacgatct
    33541 ccgttaacag tcgagcaatt tttgatcgtt gaaagtagat tcttcttaaa tttttgatat
    33601 ccgtcaaatt cagacgttcg acatggatct ctgcttcttc tgaaatttgg atctctggct
    33661 ttgttttgaa ttcagtaaat tctttattta attctatgat ataatttctt gttttaaatt
    33721 tagacaaacc taatgtttca tataacttag gcagcactac ataattcttt ttctcaaatt
    33781 ctaaatatct agctagcagc agtttttctc tatcttcctc ttctataagg taatccaact
    33841 tcatatccta cacctcaaaa aatgcctcat accaattact tgatatgcag atgaaagttc
    33901 atcttttcac ctatactgat tttatgatgg attatgcttg ttttttcatc agttattctc
    33961 attttttatc ttttttttga gaatattgca aaaaatggaa aaatttatga ggaaatattc
    34021 tatatttttt gttttattgt gttaaaatgt acaagatttg cgccatttgc gtaaacaacc
    34081 acaaaaaaga gcaggacggc caccgtcctg ctcttttact tattttaagt tccattactt
    34141 attaattggc taaacttctg cttgtttctg atattgcttg tttttctcca atgatccgca
    34201 cttctgtttc taaagacaca tcaaattttt ctttgattac ttcttggatg tgcgcaatca
    34261 attcaatata atctgtcgct gtcgcatgat cgatattgac gatgaatcca gcatgttttt
    34321 cagagacttg tgcaccgccc catttcaggc cttgcaatcc tgcttgttgg atcagctgac
    34381 ctgtatagtg tcctactggg cgcttaaaga cacttccgca tgaagggtat tctaatggct
    34441 gttttgattg acgcaattcg gtcaactcat ccattcttgc tttgattgct tcataatccc
    34501 ctgtttgcaa actgaatcga gcttccaata cgatagcacg taattcttgg actttactgt
    34561 gacgataaga aaataccatt tcgtcttttg tcaaagtttt caatgttcca tcttcaagca
    34621 aaagatccac ttcagcaaaa acatctttga tctctccgcc atacgcacct gcattcatat
    34681 aaaccgctcc accgacactt cccgggatgc cgcaagcaaa ttcaaatcca gtcaatgatt
    34741 ctgccaaagc tgcataagtc gtatcaatca gtgaggcacc tgcttccgca atgaccatcg
    34801 ttcctttgac atgaatctgt ttcatttcag tcagcatgat gactacaccg cgaatcccgc
    34861 catcttgaac gatcagattg cttgcatttc ctaataccat ccaaggtata tcatggattc
    34921 ggcaataatc gatgatttgc tttacttctt cttgtttttt cgggaatgca aggacatcag
    34981 ctggtccacc tgttttcgta aacgtatagt tcatcaatgg ttcatcaaac aatatagtaa
    35041 tcgtggctaa gctttcaact atttcttctt tattcactga ggattttcct ttcttttctc
    35101 aaacgtcctc attattctat cacgcttaat acaatcttgc cagtcttgtt caaagttgat
    35161 gacggtagtg atctttaagc acttcctcac ataattcatg acttcttaca acttcttttt
    35221 gtttaagcaa ttctgtttcc gatttaccta aacggatctg agaaaagaag gcctctacca
    35281 ttggctcaaa cccgcgtttg actagcgtgt ttgtccagtc gccaaattct ttcacttttt
    35341 ttccatcagc atcttgaatg acaagctggg tcagatcgtt tacttcataa gtaccatttt
    35401 tacttgttac ttgatatttt tctatattag cgccacttgc taaatccatc gttaaaatag
    35461 ccgtttgatt ttgtgtttct aattgtaaaa tggcatattc caaaaattcc tttgtttctc
    35521 ttatctggta ttttgttctc aaaatgggtt catctaataa atacacagct gtatcaataa
    35581 gatgtaaaaa caaatcataa aggacgaact ctgtcgtttc ttttgccgct attcgatttt
    35641 tttgcaggtg gatcaaccgc ttatctggga tacgtttcaa ttcttctacc ataggagcaa
    35701 agcgccggtt gaaaccgatc atcaaaatta tttggttctt atcagctaat tcttgcaatt
    35761 cacgaacttc atttagatgg acactcagtg gtttgtcaat ataaacatgg ataccatttt
    35821 ctagacattt tttggctaat tcataatgta cttttgttgc tgcgtggatc atacatgctt
    35881 cgattttttc ttctaaaagc tcatcgatcg tttcatatag atgttggaac ccgtactctt
    35941 tttgaattct ttcttttgtt tctttatttc ttgttgcaaa ataaaaatca agtgttcctt
    36001 gttttttcgc ataagttggt aaatatgctt tttctgcgat attccctaca ccgattacac
    36061 caatcttcat ctactttcct cctggatcat ttagttactt tcatcttaca ccatttttta
    36121 ctaaaatgag gtacaatagt atcgcaaagg taaggaaatt aattcgaagg agtgtgcttt
    36181 tattgaaatt tatctcttgg aacgtaaacg ggcttcgtgc catcgtaaat aaaaattttt
    36241 tagaagtttt tcatgagtta gacgccgatt ttttctgtct gcaggaaacg aaactccaag
    36301 caggacaaat tgatttagat cttccaggtt attatcaata ttggaattac gcggaacgaa
    36361 aaggctattc cggcacagcg atttttgcta aaaaaccagc attgaatgcc acttacggaa
    36421 tgggtatcga tatccatgat acagaaggcc gtctgattac tttggaatat tccgatttct
    36481 ttcttgtaac ctgttacacg ccaaattctc aaagcgaatt aaaacgttta gattaccgat
    36541 tagaatggga agaagccttt tataattatt tggaaaatct aaaaaaacaa aaaccagtca
    36601 tcgtgtgtgg ggatctaaat gtcgctcatc aaaagattga tttgaaaaac tggaaaacga
    36661 atcaaaaaaa tgctggattc actccagaag aaagagcggc tctttctcga ctgttggata
    36721 acgggtttat cgatacattc cgttattttt atccaaccca ggaaggtgtc tactcttggt
    36781 ggaactaccg attcaattca aggaaaaaca atgctggttg gcggatcgat tactttttga
    36841 cgagcaaaga tttagagcca ggactagctg atgcaaagat ccatacggac attatgggca
    36901 gtgatcattg tccagtagaa ttagatttga aataaagagg ttgtgaaaaa gtcgtttagt
    36961 ttcgagtatt aagaaaaatt ttgtgaaaat ggctttccac attttgcata aatttggcat
    37021 aataccgaga aactggcttt tgaacactgt ttatcgagag attgtgaaaa agctgtcctg
    37081 catcaagtaa taagaacagc caaacaaaaa tagttcctca tatttttcgt ttggttgggc
    37141 ttattaccgc agatacaagc ttttgaacac cgtttattaa gaggttgtga aaaagctgca
    37201 ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatattttt cgtttggttg
    37261 ggcttattac cgcagatgca agcttttgaa caccgtttat taagaggttg tgaaaaagct
    37321 gcactgcatc aagtaataag aacagccaaa caaaaatagt tcctcatatt tttcgtttgg
    37381 ttggacttat taccgcagat gcaagctttt gaacaccgtt tattgagaag ttgtgaaatc
    37441 agcgtcctga cctgagtatt agagaaacaa atggcaaaat agttcctcat attttgctat
    37501 tttttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga agttgtgaaa
    37561 aagtcgccta ctttcgagtc gtaagaaatt tttttgaaaa tggttcctca caactttgca
    37621 taaatttgac ttaataccga gaagtcatga tccccatcta actccaaatc acaaggaatg
    37681 agttcaaaaa ctagcttttc tcaacaaata ttacataaaa aattctatgt tttttgttaa
    37741 atttatttgc ttttttcaaa agtatgttac agtaatttcc caaggaggtt ttacagaaat
    37801 gaattttgtt gccatggatt ttgaaactgc caatcatcag ccttatagtg cttgttctct
    37861 tgcgctagtc atggtaaaaa atagccagat cgttgatgaa ttctatacat tgatacagcc
    37921 tgagactcct tttttctggc gaaatgtcca aattcacgga atccatcaag aagacgtacg
    37981 caatgcgccg aaattcccag aagtctggca aactatccaa ccatattttc aaaaaaatcg
    38041 tttagtcgtt gcacataatg ctgcttttga tacaaaggtc ttagcgggat gcttagatta
    38101 ttatcatctt gaacagccta attatctttc tttatgtaca gtgaaaagta gccgtcgcct
    38161 gtttcctgaa tttccaaatc atcgattgaa cactgtgtgc gaaaatttga acatccaatt
    38221 agatcatcac catgatgcat tggaagatag tcgtgcatgt gcggaaatcc tgctttatca
    38281 agaaaaaaaa ttcgggacag atccattgaa aaaacttgtg actataaagt aaaagaatac
    38341 ttatgaaaca cacaaagagg tcgtgagcct gccgtttcac tccgagtcac aaggagcaat
    38401 ttcaaaaaat agcttctcat gtttattaaa tattgtgact ggtgaccgag gagctggcag
    38461 gagaacaccg tttattggag aggttgtgaa aaagctgttc tgcatcaagt aataagaaca
    38521 gccaaacaaa aatagcttct catatttttc atttggttgg acttattacc gcagatgcaa
    38581 gcttttggac accgtttacc aagaggttgt gaaatcagcg ttttgacctg agtattagag
    38641 aaacaaacgg caaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggtca
    38701 gctgattgaa caccgtttat tcaaaaaaaa tggactgcga cacgactttt gtcacagtcc
    38761 cttcgtgtgt ttttctgcct tctcaaaagg atgacagttt ctcactcttt tcctatttct
    38821 tctattcaat caaataactc attttatata catctaaaaa ttcacctaga tctgttcttg
    38881 cccctctagg catgaccgct tcgatttgga agcccatttt tcggtaaagg tgtacggctc
    38941 tttcattacg aacttgaacg tcaagctcca atcgaaaaag tacacccatt tccttcgccc
    39001 aactgatgat ttcttctagc ataagagtgc ctagtcccat tccccaatat tcctgcagaa
    39061 tactgatacc gacttctccc acgtgagaca aacgaaattg actgtctgcc ttgacagatg
    39121 ctgttccaat aatcgtccct tcatagattg caagcagcaa taaattatta ttgctttcgt
    39181 atatataatc caactcttct tctaaggttt ctggtggtaa caggagttct gcttcatcca
    39241 aaactaaaaa ttctgtctcc ttattaactt gtcccatcat caaaagaatg tcttttgcat
    39301 ctcctggcac tgcttcccgc aagatgatcc caacttcttc actcatgatt ttcagcccat
    39361 ttttctagaa ttcgctgttt gaaggtttca ctttgctccc cgtatgagat tagtttcaca
    39421 taccgctttt ctaaatctgt atcacttttt tctaaaatca gttccaaata atcctccggc
    39481 agtgcttctt ctaatagatt tccccattca acaacggaaa gcccatcacc ttcaaaatat
    39541 tcatctaacc ctaaatctgc tccgctagct gctacccgat aaatatccat atgatacaac
    39601 ggaatccgac cttgactata ttctctaata atcgtgtatg tcggactttt gatcatttgc
    39661 gttatcccta atccttgggc aattcctttc gtcaacgttg ttttacccgc acctagatca
    39721 ccagtcaata tcagattgtc acctggcatt gcggaatgac caattgcctc tgcgaattgt
    39781 tctgtcattg ttaatcctga tagttcgatc atttttcatc ttctcccgct cttctttctt
    39841 tttgccagta tatcgcgtaa tgaagttttt ttaaagaaaa aatgaactag tcaagaacag
    39901 cagctgctgt ttttggctag ttcgcgtgta tttgttaatc aatcaatgtt tgtgctgctg
    39961 tgataatcga taatttatat acatcttctt catttgctcc acgagacaaa tcagaaaccg
    40021 gtttgttcaa gccttgtaga attggtccga ttgcttcaaa gttaccgaaa cgctgagcaa
    40081 ttttatagcc gatatttcct gactgcaatt ctggaaatac aaagactgtt gcttgaccag
    40141 ctacttctga atctggcgct ttcagttgtg caactgaagg aacataagaa gcatcaaatt
    40201 gcaattcgcc gtcgatcatg tattgtggcg ctagtttttt agcgatttta gttgcttcaa
    40261 ctactttgtc tacttctggc gatttcgctg agccttttgt cgagaagctc atcattgcta
    40321 cttttggttc gatatcgaac aattctgctg tttttgcaga atctacggcg atttcagcca
    40381 attcttgcgc agttggattc acgttgatcg cacaatccga aaagacatat ttttcttgat
    40441 ctcttccacg taccatgatc atcgctccgc ttgtccgact gatgccaggc tttgttttga
    40501 tgatctgaag tgctgggcga acagtgtcgc cagtcgaatg gatcgctcca gagaccattc
    40561 cgtctgcaat ccccatatac gtaagcatcg taccaaagta attcacatct tttaaaatct
    40621 tttctgcttg ttctttggat gcctttccat ttctacgttc aacaaaagta tccaccattt
    40681 catcccactt atcgtaatta tcagggctga tgatcgtaaa gctagatgtt ttgattcctc
    40741 tggcatgcgc tgcatttctt atttcttctt cgttccctac taaaatgggt tccacaagtt
    40801 cttctgcttt caaccgggca gctgcgccta aaatacgagc atcagtagct tccgggaaaa
    40861 cgattttgat cccacggcga acaattttaa atcttaaact atcaaacaat tccacaagat
    40921 gacctccaga cgtttttata tgtattatca tacactattc cagaaaaaaa gaacagttta
    40981 ttttatctgt atcagtcaaa aatgctggaa aaattaagtt tttcacatac aaagcgaact
    41041 gcactataac aggaaagcgc tgttttatta cactgtttca ggcagttgcc aatcaatcgg
    41101 ttcttctcct aacgcaagga gcgcatcatt cgttttcgag aagggtttgg aaccaaaaaa
    41161 gccacgactt gcagataacg gactgggatg tggagatgtt atgatgatat ggcgactctt
    41221 atcgatcatt ttgatttttt cttgtgccgg ttttccccac aagataaaca cgattggttt
    41281 ttcccgttca tttaattttt caatgatcgt gtctgttaat ctttcccaac ctttcccgcg
    41341 atgagaatac gcttgccctt cccgaacggt aagaacggtg ttgagtaaaa ggactccctg
    41401 tttagcccat gacactagat ttccatgttg aacaggtgcg attccaagat catcataaag
    41461 ctctttatag atatttctta acgatggcgg gatctttacg ccaggttgca cagaaaaaga
    41521 taagccatga gcttgatttt ctccatgata aggatcttgt cctaaaatca ccactttaac
    41581 ttcttcataa ggagtcaatt ccaatgcttc atagatatga tacatatctg gatggatctt
    41641 ttgggacgca tattcttttt ttaaaaattc tcttagatgc agataataat cttttgaaaa
    41701 ttctgaggac aaaacttctt gccaactgtt atgaatgatt tctttcaaaa actccacctc
    41761 ctaataaaaa gaataactca cattttcttc aatcacaagg gacaatcttc accctcagca
    41821 cttgattaat atggtaaact aggaacaaga agcagaaagg aacgataatc attatgatca
    41881 aattaattgc atcagatatg gacggcacat tattagatgc ccatatgagt gtttctcctg
    41941 aaaatgccga ggcgatacgt tttgcaaatg atgccggtgt tgagttcatg gtcgctactg
    42001 gtcgaaacta tcaagaagct cgcgcagctt tagatgaggt aggaatcgat tgtgcgatga
    42061 tcactttgaa cggggcacag gtatttgata aagaaggaaa ttcattgttt accgtgtcga
    42121 ttccaaataa tcaagcaatc tctgttttag atattctaga tgctaacggt atttactatg
    42181 aagtagcaac caacgatggt ctttattctg aaaatcaagc gaaacgaatt gaaagttttg
    42241 cttccatggt ggctacccat ttacctcacc ttacgtataa aatggcgatt gccatggcct
    42301 cagctaagct ggagttattg cacatcacat acgtggacag tattcgtgct attttggaag
    42361 aaaagaatct ggaagtttta aaaattattt gttttcatac agaagggcca gctgtattag
    42421 gtccagttgg aaagcagatt tctcttttag gcgatctagc cgttacttcc tctggtcaaa
    42481 acaatatcga agtgaaccat aaaaacgctc aaaaaggaat tgctgttgcc catgtcgctc
    42541 acgagcgtgg gatttcttta gaagaagtca tgacgattgg cgataacttc aatgatgtca
    42601 gcatgctgca agtagcagga gtcagtttcg caatgggaaa tgcagaaatc gaagtaaaag
    42661 attatgccaa atatacaacg gacacaaacc ttgaatctgg cgtaggaaaa gccatattgc
    42721 gcgcgattgc agaagacttg tagttttatc tagaaaagag ggatatagat gtcagaattt
    42781 ttcattcagg aaaagcagct tggacgtgca actcggacaa ttgtcaaaaa cgaaaaagga
    42841 cattccctgt ttttgatggt tggtcgctgg ggaacaaagg gcgatgcatt gtctctttat
    42901 gccatgaatg gcgatctcgt tgctcacatc aagcagataa gcctgacatt cggtacgcgt
    42961 tttgagctgt ataaagagtt cgaaaaggtg ggaaccatgc agaaaatatt caattggccc
    43021 ggtgactttt attatattcg tagacttcac tggacagtcc aaggtgatat ctataatcat
    43081 caatatcaaa tccatcattt caatcagcaa atcatgaaaa tggataaagc aacacttttt
    43141 tcaggagatt actatgtatt ggatgtctca aatgatgaag atgctccagt ttgtatttgt
    43201 attgctgcgg ttttagatta ctggctctac cgtaaagaca aaagtcgcga taagcctgtt
    43261 tcttaccgac ttaacttaag ttaaaaagat gtacctgaaa tgcggaaata cgcatttcag
    43321 gtacatcttt ttagatattc ttttgcatat gatcataaag atgaccactg atcatccgct
    43381 gaccgacaac tttgaagtca tggcgttcgt ataatttttt agcctttgga tttttttcat
    43441 ccacacttag accgatgacg gagcgattag ctttcttcgc caatttaggt aatgcttcta
    43501 ataatcgcga accgatgcct tgtcctctaa aatcttctga tacactgata gagtccaaat
    43561 accattcgtt gggaaacgtt tctttatcaa tgaacaaacg tgtagacgca tctagctgat
    43621 atttctctaa gataggtacc agtggctgat cgatgattga ttcttcttca tctgtataac
    43681 cgtaagcaac tcctgcaact ttaccgtcta tttcttctac gatacctcgt gaataactgt
    43741 aacgataatt tggatcagtg attgcttctt ctaaaatctc taaagtcttt tgttcccctg
    43801 tctgcttcaa aaatggcagt tccatatctt ttagaatcac taagatcaat ggggcgattt
    43861 ctggagcatc ttcttttctc gcaaaacgaa tcatagtttc ctctcctttt ctccattcta
    43921 acatgcagaa aaagaaatcg caaaatcgtt attttaaaaa atcttcaatc ttttcagtaa
    43981 tgaaataacc atgagtaacg attcttgccg cttcagttcg gcaataaggg cagacgactg
    44041 gcatttcttt atcattatgt tcgactacat tattgacttc ttcgtatgca aatttttttc
    44101 cgcaattagg gcatttcatt ttttccatgt tatctcaact tcctttcatc tttactctct
    44161 tagcttactc ttatattgta aaaaagagaa gcaatatcct tcttctatgc agatatccat
    44221 acctgacggt cctgttctcc atctattaat cggactttat aataaggacg ttcatctttg
    44281 actagaagtg tccactcttt cacttttgct tttttcccgt agtagctgac tgcttccttc
    44341 atcgctgctt gcggttgttt gaactcttcc gttttttcta tatcaaaaag atgttctgcg
    44401 gccttttccg tgtcatttcg tacggttgtt ttcccagtgg atggattgac tgctatgact
    44461 tgtccttcac aaaaaaactg gtattcatat gttgcattcg taggattctc caaatctttt
    44521 tcaactgcaa aatgaatagc agatatgtta tgttcttttg tttcgttttt tacgattgct
    44581 gctgcttttt tcaatgagat ccgatattcc tggtcactaa tatcatccat gtgaaaagcg
    44641 gcattgagca gtcccgtcat accggtaatc cctattacga tgagggcagc aacaccaaac
    44701 atcatttttt tcattcttca ttctcctgtt tctttttttt aaagaatatc aagaaatgaa
    44761 cgaatgaaaa atcgaatgaa acgaaaaaaa agtgacaatt ttgttatctt tattcgtgca
    44821 gttctaatgg caatccgtct ggatcaaaga aaaacgtcat tttttttcct gtaaaggtat
    44881 cttctctaat cggttctgat tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa
    44941 tatcgtctac tttgaaagca agatgacgca agccacaagc ttctggatag tttggtcgtt
    45001 gtggcgcatt agcaattcca aaaatctcca attcagcact tcctagtttc agatctaatt
    45061 tatagtcatc tcgttctttg cggtaatttt ctctgattac ttcaaatcct aatttattaa
    45121 cataaaaatc acgtgatttc tgataatctg aaacaatgat ggctatagga tggatcgttg
    45181 taaggttcat atgagcactc ctattcttcg tttaagctcg gatctatatt aatctccagt
    45241 tccattgtaa atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag
    45301 gcaaaaaaaa cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg
    45361 aaagtaagaa attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt
    45421 ataattgaaa gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac
    45481 agaacacact acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt
    45541 ttagctcatc agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt
    45601 ggctgccagg tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt
    45661 atcggtacca cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac
    45721 ggtattttag gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac
    45781 tatttaagta tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta
    45841 agtaaagaat ggaatcataa tatggcttca aaaatccgaa aattaactgg aaaaagctgg
    45901 tttatcgcaa tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt
    45961 tatacagatg atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct
    46021 ggtatcatca gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt
    46081 gctcaaatgg tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc
    46141 gtgaacagct ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat
    46201 aaccaaaaag aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa
    46261 cacgcataaa aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa
    46321 acacgtataa acggcggaat cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    46381 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    46441 agcctctatt ttttatagta attccttcag atactttctt gcctcatcat tgaaacgcag
    46501 tgattcttct aacttctgat aatattccaa cgcttgtttc tgagcttcag taaaacgata
    46561 atgttttcct ttccatttgt cgattgtctc ttgcacattt ccagattgga tatctttcac
    46621 tactagttgc catttttctt ttgagactgt acggtctttc ttatcagcag attgtatagc
    46681 ttctcgttct ttacgcaata cttttaaaaa accatttctg actatccgag gaatgctgtc
    46741 gaagaaccct tctgtaaaac catagcgaca atattgcttc caaataaata gatagatttt
    46801 tggttggatg aatcgttggt ttttcgcaaa atattccatc tgagcagtga cttctggtgt
    46861 tcgttgacgt tttgtctttc tcaacacgga aataaatgtt tcaaaataag cattaggatg
    46921 tactggcact tcttcatata cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt
    46981 gatcaagaat tcttctacat cctttactaa ttcttctgca aagtcgtcaa atagttcttt
    47041 ttttatctgt cgcttttttg actgcccatt gatgtgttca cttattttct tgatttcatt
    47101 ttccaatgct tgataatccg ctatgtaaat cggcatttct acttggcgaa attctgcgag
    47161 ccgctgagct aattttatat ccggtaaata tggctgtttc atccgacgat tttggtacaa
    47221 gacaaaacag tatttttgcc atagtccttc aggaaaatcg atattcttct gcttaagcca
    47281 taataattcg tctaaggcca gatcaacttt tgtcattccc tgatggatcg aagctgccac
    47341 tgtcggagag acatgcaggt gatcggcaaa gtggttgatg cctagtttta ggatctttcc
    47401 ggtttttggc gatttaacga tgtattgcac ttttaattct ctaccacact cacaatacaa
    47461 tttttgctgg cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca
    47521 atctgttgca gctaaataat gttcttccat aaaacgagag ctgattagat atcggcggtg
    47581 cttatcgact agttcccgct gttttttcgt taattttgca tagatctccc aacgttgact
    47641 gcttgtgtat tgtttagaaa ttttgatggg atagcttttg ctgatttctt ttggaaacca
    47701 ttcttcaaag acggattgcc attcttccac ggctgtttct ttcctttcta attacaaaaa
    47761 gctggctact aacataacgt tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt
    47821 tccggcattt ccggaaatat cttctttatt ttctactgct gcttcaactg cataagtcaa
    47881 agaagaagca atgtcttcta aagacataga aggcgtaccc actggacgat cgatcacttg
    47941 atcgggtgag aatggcacgt ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat
    48001 caaatgaagc aaacgataca tgatatcatt gcagacaaat gttcctgctg tataagagat
    48061 gtacgctggt agaccatgat cttgtacatt ttttgtcatt gctttgattg gcaatgaggt
    48121 aaagtaagcg gctggaccgt tttcttctaa agccgtatcg aatggctgat ttccttcatt
    48181 atcagggatt cttgcttctg ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg
    48241 cccacctgct tgtccgacac agatcacgac atctggttgg tgctgttgga tggcttcttt
    48301 gactacttga ctgctttttt cgaatacggt cggtacttcc actttgatga tttcagcacc
    48361 gctgatttca tctggcattt tttttactgc ttcataggct ggattcactt tgtccccacc
    48421 aaatggatca aaacctgtta ctagtacttt catctgttgt tcctccgtcc gattgattta
    48481 acatatggtt atcttttatc ataccatttt ccttgaacag cgtaaataaa ggaaacaata
    48541 gtttaaataa cataacagat caaacttaat atattcttat aaaaaaatag tgttccacta
    48601 caatgttaga agaggttgcg acaattattc tgtcacaacc cctctcttct attatttaaa
    48661 tagaatcata taagcaattt ggaaaactag cataaataac gcaataaaca actggttttt
    48721 gatcactccg tatttgtctt tcatctccag catcgctaca ggcacgatat tgaagttagc
    48781 agccatcggt gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca ttcctattaa
    48841 cgctggattc gctccatgac taaatacgaa tggtgcaccg atcccaaccg tcatcacggt
    48901 gatggccgca aatgcattcc ccatgattac cgtaaatatc accatgccaa gagcgtaaat
    48961 gatgatgccg atgttcacat ttccttctgg aacgatcgta cctactgcat tggaaatcac
    49021 tgtcccaact ccagcgcttg taaataccgc acctagagaa gctaaaagca ttggcaacat
    49081 gcttaacggc cctactgtgc ccagcatatc tgctgcatca tctaaaaatg tacttggtcg
    49141 attatctcga gaaaagaaca tcaagattag catggctgca aaaacaccga cgcccacgcc
    49201 gactaaagca cctaaattgg taaatagagc aaacaagatc gcaaagacac cgatactcaa
    49261 agctggaata aaaattttca ttcctagttt gtctgacatt ttttccatat attcttttga
    49321 tggaagccgg ctttctcctt tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa
    49381 gatcaagcca ccgttcacga ttgccggaag ccatcttcct cctgcaataa ctacccctaa
    49441 gaccgtccag aaaaaggctg acccataacg atgaggattg gttttatctg ttaaatttct
    49501 tactccagtg taaatcaata cgagtcccat cacgatataa aggatctcca gtaatttatc
    49561 gcctaataaa atatcaggat ttgtaaaaaa gtccatctta ttgccccttt cctggataat
    49621 atttcttcgc taatttcttt tctttaagat agaagtagac acttgccaca actaaagccg
    49681 tgatagccac tggaacttcc actaaagcaa gctgtcccaa tgttacgtca tatcctagat
    49741 ctttcagtgt tgattggact agcaacgccc ctgctcctcc gatgaatagt acttggccaa
    49801 agaaccagca gacattttcc atagctgaag ccataccttt tagttcttct tcatgcttag
    49861 gatggacttc taaccctttt gattcgatgg tccccaatgc catcggtaaa atgattggac
    49921 gaacaaatcc agcaactcca ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga
    49981 tcccataaat atctacaatc ataccagcag atactttctt gaatcgcttg atcaaggaag
    50041 ccgctgattc tttcagacca tttctttcaa gcgttcctgt tgccaacata atgatgataa
    50101 aaatcgccat cccgcgattg tttacaaaac ttgtacctaa aagttctaac attccttgaa
    50161 tgccaagacc gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc aatatcgagt
    50221 ctaatttaaa tgcaaacccg agaataacaa tcaagacgcc taaaaggcta agataatttt
    50281 ccatatattt tcctccataa aatgacaata tttttttaat tataaagcaa aacacataaa
    50341 aaatatagtt gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt
    50401 attatataat tcaccttact tattgtaaga taaagctttt tttaatttat tgcctgctaa
    50461 tatataaaaa taaggtctag ttactgtttt tttaaatgat taataaataa caactccttt
    50521 ctatgaaatt gcaaagcatt catcttcttt tctgctaacg atatatccgt caatgcttgt
    50581 tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa
    50641 aaagatatac gatctgccac cgggtagtaa aaaagaggat gatcattcca ttaggtcttt
    50701 gaagggatga ttctcctctt tttgtgtatt ttttatattg aaggaggaac gtatgtatgg
    50761 catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat
    50821 ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattgga atgattgcta
    50881 gtttttactt tttgtcaaag gcaatcaaaa gtttgcctat gagtctctct tatcctattt
    50941 ggacagggat tggcgcagtt ggttcggtca ttattggtgt ggtcttgttt catgataagc
    51001 tgaatctttc cacttggttc tttgtcgggt tgctgctcat cagcatcatt ggcatcaaaa
    51061 tcactagcgg tcactagata cttagacaag cagacaagga ctgatcagaa aaagcggggc
    51121 tttttatttg atattcctct ttctatgaaa aaacaagaaa ataagactaa tgacctagcg
    51181 acttctctac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg aatatttttg
    51241 aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc cctcagcgat
    51301 gtcagtttta gcttatctcc tggcaaaatc gttggattag taggtcctaa tggcgctgga
    51361 aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagaagg aacgatccgt
    51421 attggtgaaa atgagatttc tccctctagt cataaaggat tagaacaagt aggagcattg
    51481 atcgaatatc cgggtatcta tccattctta acaggttatg atcatctgaa gttattctct
    51541 gaaacaaacg atgtatctgc tatcgacact atcgtgaaac agctcaagat ggaaaaatac
    51601 attcacaaaa aagcgaaatc ctactcgctg ggaatgaaac aaaaattggg aattgctctt
    51661 gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg tttggatcca
    51721 caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg cacctctttc
    51781 ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct gatcctgatc
    51841 aatcaaggaa aaatcatcaa acaatgtaca atggctgaat tgaaccaaac agcgcaagat
    51901 ttcatcgtta tctctacgac aaatgatcaa gctgctcgaa cgatcttgac agacgctgga
    51961 tatcccttgg aagatcctga taaaatcaag cttctaaaaa aaaacgaaga gttattagga
    52021 actgttatcc agttattgtc tgcagaaaat gtccatgtga cagatgtcca gcatctgacg
    52081 aatgatttgg aatcttccct attggaaatt ctggattcag taaaggggga agaagaaaaa
    52141 tgacgttgct tataaaacaa gaattattta aattgatcaa gaaaaagtca actgctgttt
    52201 tatctgttct attagttgct ttgctcatag gaacagcact tctagccaaa aaatacacga
    52261 cgatcattga tccagttgaa atgactgctc agcttttcag tgccacatct tggattgtct
    52321 ttatcatgat tgctgctgca agtacgatca tttcaatgga atcacaatat ggtacattga
    52381 aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagtaaa tggatcacat
    52441 tagttattta ttctatttat ttataccttt tagctattat tgtcaccgtg ctgatgaaac
    52501 tgatcctttt cccttcgatt tcctttacgg agaagatttc tactggtcag acactgatcc
    52561 aaagcttatt cacttatact ttgggtagtt atatcggttt atggctaatt ttaagtttag
    52621 tgttgatgat tgcttgtttt atcaatagtt ccggtgcctc catctcagca gggatcgtct
    52681 tttattttgc ttcatcaatc atatctggta tcctcatcgc actcattcaa aaatgggagt
    52741 ggatcaaatg gaatccaatc agtatgctga atctgcaaaa ccaaattggc aacgaagaga
    52801 ttatgaagca attgactcat ctttctacta atcaaatgct gtttggcaat ttagcttaca
    52861 ttgctttgtt cttagcattg ggatacctcg tattcaagcg aaaaaatgta tgatggttca
    52921 ttaacaaaga agttgtgaca gaagtggcca gtttcaagaa atttcggctt atttttagaa
    52981 gaagctactt cttgaacacc atttattcaa aaaaagaagc gatgacacga atgttgtcac
    53041 cgcttctttt ttgaataaac cacaggcttc acagcctctc tttatacttc tatacgtgaa
    53101 gcatcccagt ggcaaataaa accattgcga ttactccgac catcgtcaca ccgataagta
    53161 aaatcttatc taagtggata tgttgtacag gttcgttttg ttctttacgt gctttgaggt
    53221 aaaaataaat acctggtaag aaaagaacaa gaacaagcat cacttcatta aaggctacaa
    53281 ctagcatggc ccatacttga aaagcacttg ctaaaaaccc tactaaaaat tggacccact
    53341 ctttccgctg ataagaaagc ttcatttggt acagccctaa gaaaagccac gtgaagaaaa
    53401 tcgctgctgt accaagtgta taagcaaagt tatatgcact gtcagtgaat aataatgaaa
    53461 gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt
    53521 tgttgagttt tccccatgct ttaggcaaaa gtccgtcctc agccatattc cccattgttt
    53581 caatcggcaa gattgtccat gagagccaaa ccccaactaa agaaatgatc aaaccgccgt
    53641 tgatcacgac cgccccccat ttaccaacca tcatttcaaa tacatatccc atagcaggtt
    53701 gcgctttgat cgtttgcagc tgattcatcg tcattgtgcc atatggaagc atggaaacca
    53761 agacgtaaat aatgatcaaa ccgaatattc ctagaatcgt tgctttccca acatccgatt
    53821 gtttttttgc tcgtgaacct acgacagaag ctccttcaat accaacgaat acccagagca
    53881 tcaccatgat gcaactcaat acttgactag aaaacgggat cggctgccca ttcgcattca
    53941 atatgatatc gctagaaaag ttattaatga attgttggat cgaaaaggtg ctgatagcta
    54001 ctacaataaa taaaaataat ggcactaatt tacaaaatgt gacgatagca ttgacaaagc
    54061 tagcattttc aactccacga ttcactagcc atgcatatcc ccataagaag atcgtactca
    54121 tcacgatcga tgccacattt tgaccacctt cgaatacggg gaagaaataa cctaatgcac
    54181 tcatcaacaa tgttgcgaac gccacgtttc ctaaccagca tgtgagccag taaccccaac
    54241 cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt
    54301 ttaaatctgg acgtttttta ttgatattat tgatcgataa ggagagcatc aacattgctg
    54361 ttcctacgag tacccagctg aagatagctg ggccaccagc cgatccggag atgtccgtca
    54421 taataccaaa tacgccacct ccaatcgctg aaccaatcac taaggcagca agtggaatca
    54481 aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa gtaaaaccgc
    54541 ttttatcaaa aagtataaca aacctttttt atttttactt aattaataaa aactttaata
    54601 gaaatataat tctaccgact tttttctcat tatacaagca aagcgcctag tccctttcca
    54661 aattacacca ccaaaaaaac ttaaaaagat gctatactaa agggaaagaa ttgggggatg
    54721 atccatgaac attttatata tatcgatttc aggaaatacg cgtgcttttg ccaaacatct
    54781 agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa
    54841 agaaatccat gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc
    54901 tacttattta gacggcggga atgggttaga caacggtgac acagaaatct tgaccgaaac
    54961 gatgcgcgaa tatctggaat acgaagacaa tcataaactt tgccttggtg tagtcggtag
    55021 cggtaataaa aacttcaaca atcaatattg tctgacagcc aaacaatacg cacaaagatt
    55081 cggctttcct ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgcgt
    55141 ttatcaaatt ttggcagaaa aaaataaata acacaaaaga agaagctgaa aaataaattt
    55201 cggcttcttc tttttatgtt ctctattgtt tctattctta ttaagctttg acatccatca
    55261 caggtgcttt tgctttcgct ttacctgttt tttccaagac aaagtttttt acacgatcca
    55321 tattggttac caagacgatc atggaggttt ctttgtctgc tgctttgatt gcttctaagt
    55381 cagcttctgc tacaagcgta tcaggtgtca cttgctgtcc ttctgttact ttcacatcga
    55441 atggcgtacc gcctaagtca actgtgttga tgcccatgtg aagcaagatt tctaagccat
    55501 ttggtgtcgt aatgccgata gcatgttttg ttgggaaaac agaacttatt actccagtaa
    55561 ccggggaata gatatgtccg ttttctggtt ctacaaagta accttcaccc atcatttttt
    55621 gtgcaaaaac tgggtcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg
    55681 ataagatagt cacactttca gctgaagctt gaggtactgc tttttcaggt acttcattta
    55741 ctgtggctgt ttcggtcgct agttcttctt tttctaaacg gtctttagga ataccgaaga
    55801 agaatgtagc aacgaagcca ccagcgtaag cagccaataa accaaggata tagcctaacc
    55861 atttaccatc agagatcaat ggaatcaaag cggctcctga aggtccaata gcgattgctc
    55921 cgacatttcc gatcattccg atcactgccc caccgatacc tccaccgata caagcagtaa
    55981 taaatggacg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctaaga
    56041 ttccgactgg taaagcccct ttgatcattt ctgttaattc tttgtctttt ttgcagcgga
    56101 tccataatgc caatgctgca cccacttgtc ctgctccggc catcgcaagg attggaagca
    56161 ataaggtacg accagtttca tcaatcattt gtacatggat tggcgtcaag atctgatgta
    56221 gtccgaacat gaccattggc aagaataatg tacctagaac gaagccggca aatgctcctc
    56281 caacaccaag tacccagttg atcccaccga ccatgccgtc agaaatgaac cctgctaaag
    56341 gcatgatgaa gaaaatttcg atcagaccga tggcaatcag ggaaagagtt ggcgtaacga
    56401 tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaataacc
    56461 aaactgcgaa gataacacct aagatcccgc cttgtccagt tgcaagagct tcaccggtaa
    56521 acaagttttt cagtggtgct tctggattca tccctgtcag catcacaaca gccccgataa
    56581 ctccaccaag cgttggcgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa
    56641 ctagatagct aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg
    56701 ttcctgcgtc tagtgttcca gctgtgacta agtttgctaa aactgcggca atccctgcca
    56761 cgatcccagt gccgacaaat gcagggatca ttggtacaaa gatattagag atgtctttta
    56821 ataccgattt gattttagag ggtttttgtt tcgatttttg tgccgctttc atttcttgtg
    56881 cccgttcgtt tacacggtct tttccagatg cagaagaact tgctgtagtt gcactagcag
    56941 aaggaagttt ttctcctaat ttcacgcctg ctaagtcaac catttcctgc gctactttgt
    57001 tcactttccc aggtccgatg atgacttgga gctgttcatc ctctactacg ccaagtactc
    57061 ctggaatcgc ttttaattcc gagacattta caagatcatc attgatgacc ttcatacgta
    57121 cacgagtcat gcagtggaca atgctttcaa cattgcccat accgcctgct ccttgataaa
    57181 tttgttttgc cagttcttcc aaagtcaatt cgttttttgc catggtcttt tccccctcat
    57241 aaggttcctt ttacaaaacc gtttgcttca gctaattttg cttgagctgc ctctttatca
    57301 ctatttgtca aaatcatgac gatagccagt ttgacatttt gatctgcttc ctcaaagtac
    57361 ttttcagctg tttcgtagtc agcttctgtc gcttgcatga tgattcgttt tgagcgttca
    57421 attagttttt catttgttgg tttgacgtcg accatcaagt tattgaatac tttaccgata
    57481 ccgatcatac tgatagtaga aatcatattc aaaatcagtt tttgtgctgt tcctgacttc
    57541 aatcgagtag agcctgtcaa aaattctggt cctgcatcga cttcgattgg gaaatccgca
    57601 tgctcgctga tttctgcttg tttattgcaa gaaatcgttc ctgtaacagc accaatcgat
    57661 tttgcgtaat ctaatccacc aatcacatat ggtgtacgac cgctagctgc gataccaata
    57721 actatatcat tttttgttaa attcagcttg attagatctt cttgccctaa ttcataagaa
    57781 tcttcggctc cttcgacggc tacagtcatt gcttcttctc caccagcaat taggcctatg
    57841 accatttccg gttctacacc gaatgtcggt acacattcag cagcatctaa tacaccaagt
    57901 ctaccactcg ttccagctcc catataaatc aagcgaccac cttttttaaa ggattcaatc
    57961 gtgttggcga tcactggctc gatacttggc agttcttttt caacagcagc ggccactttc
    58021 tgatcttctt tgttcatgag ttggactgct tgagaaatac tcatttcatc taatccaaaa
    58081 gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc
    58141 ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg atcagcggta
    58201 gttcttcttt gatgactttg gctactacat tcactttttc atccgctggc aaatcatatt
    58261 tagttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagatc
    58321 ctttcatacg cttagctgta tttcttactg ggattggcgg aatctctcga aaacgcgcat
    58381 ctgcgcttcg tatgacatct cttgcatcat cttgccgatt cgtatgtttt cctaatatat
    58441 attcgtaaaa aacttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga
    58501 aagaagcaaa ttgctcctga acgtgttctg acagacctgc atctccgata taaacaatat
    58561 ccgtgttact tgctaataga tcaagagcac ctgccagagg atggatccct cgatgttctt
    58621 ccaaagttgg taatccttga tacagtggac ctctcaactc tgtatctcct ggtacaaagc
    58681 cttgtacaag gaatccttga gttttgagcc attgattttt ttcctgatac cattctttgt
    58741 ccaaccctgt ttcaagacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag
    58801 ccgctgcttc tttcaactca tcgatatcat taggcgtgat cgtgctggca ttcaaactga
    58861 ttttcaattg atgtgaccag tcggcgatct gctgattaga aatatgataa tccattctta
    58921 accctgttac accaattgct ttcaagggtg ttagatcttc taatgaaaag ccagctcgtt
    58981 tcaatgcctc tcctgaaata tcgaccatca actctagttt attctttttc gcttcgcttc
    59041 ctagttcgat caatcgattt tgataagccg aaacatcatc ttccggtata tgcatggaag
    59101 tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc ttctcttctg
    59161 acaaagaatc attcataaat attgaaaagc caaacatatt atccctcttt ttctgtatcc
    59221 gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat acttttgtta
    59281 aaataattaa attttatttc taaaaattgg atttataatg ccaaaagaat tgacacagcc
    59341 acaaaaaagg cactgctaag caaaagcagt gccttgaaac attttattta aataacacaa
    59401 aagtctaatt actcataaga aattaattct gtattgtcta ccatgaatgt aaagaaccct
    59461 tcattaggca ttggatgaaa ataagcattg atcactctta tcaaggcatt atgacagact
    59521 aacaagtaga cattttcttc gtcttccatg cattctttca ataaaggaac gatccgtgcg
    59581 tacacatcta agaccgactc tccatttggg aaacgtacag caaacgctaa acgcgctttt
    59641 tgaaagtttt catctttgct tggcagtccg tcataatccc caaaatccat ttctaccaat
    59701 cgctcatcag cggtcatcgg caaattgaga cgatcaacta ctgcttgtgc tgtatctctt
    59761 gctcgttgaa gaggggaatg gatgattttc gtgattggaa caggcaaatc tactaccttt
    59821 tctgctagtg cctgtgcctg catctgtcct ttttcagtca gcggcacatc tgctcgcccg
    59881 caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc tcgttacata aagcatgtga
    59941 ttctctcctt cgttgacaaa gttctcctta accatacctt gttgtctttg agattacaag
    60001 gtttagattt tctcaagaga aataatctca gtagatactt tatctagaaa ggtttgatca
    60061 tgttccacta gtaacatagc aggtttcacg ttcaaaatca gttgttctaa ttgttcttga
    60121 ttgaagacat ccaaataatt caatggttca tcccatatat agagctcagc tggttgggat
    60181 aaagatttag ccaattccac ttttttccgt tgtcccatac tcatctgttc gattttatta
    60241 tgaaaaacat ctctttccat cccaagcttt cggagattat tcaaaaatgc ttgatagtct
    60301 actcggtttt tcttcgcgaa ttccgccaat gtccctcgat tgtcttcata attttggcgt
    60361 acgtaactaa tggtcaaatg tttgggctgg gatttttctc ctacaacctt gccgttgaat
    60421 gtccccagaa gataatggat aatggatgac tttcccgcgc cgttaggacc tgaaattgcc
    60481 atccgctgat gaggttcgat cgtaaaatga actggctcga ataacagatt ttcataccct
    60541 aattgaagat tttctacgct taacagccgt ttatgatgag acacttggct attcatcgtc
    60601 aaagcatcga tatactcgat atcttttaat aatttttctt tctcactgat ctgggtttcc
    60661 atccgattca cgattgcttt agatcgtttc atcgtccgtg cagcatcagc gcctactgct
    60721 cctttattca ctcgcctaga ttccgtatcg atgaatccga cttttttctt tgttttgtcc
    60781 ccttctcggg aacgagacca ttctgctttt tcagctgctg ttttctttag cctgctgact
    60841 tcttttttca atttttcatt tttagctatt tcaaattcat cacgaagttt tttctgttct
    60901 tcatagatag agaaattccc ttgataaagt tccagttggc tcttttcgat tgctaaaaca
    60961 tggtccacta cttcatcgat aaatccccga tcatgactca ccacgatgaa tccttgtttt
    61021 ttctttttca agtaagccgc tacttgtttt ctgccagaga tatccaaatg attcgttggt
    61081 tcatcgatta acgggaaatg agtgtcatcc acaaataaga gtgccagtag gacttttgtc
    61141 ttctctcctc ctgatagtgt gctgaattct ctccataaga tttcaggatc tgtttgcatc
    61201 agttggagct ctctttcaat ttcccatatc tcaaaatcca taatctcatt taacacataa
    61261 taggtcaaac gctctttatc ttttgtttgc tgcgggaaat agacaaacgc ttgctgatgg
    61321 atcacttgcc cttgataagg gagtttgttt tgcaggatat tcagtaaagt tgtctttcct
    61381 cgaccgtttc gtccgataag tcctagtttc cattgtgtgt caaagtttag attggcttgt
    61441 tcaaataata atgtgccttg gctgtcatag ccgaatgtca gattttttat ttcgatttta
    61501 gacattattc catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag
    61561 gatgcaaggg ttggggcaca caaaaagacc catacatcga aagaaccaag ccaatcccga
    61621 aaatctgcac actgctccct tgtctccaca aggtacagtc tgaatgcagc tttcatcaat
    61681 cgacttaatt tttcaatgca cggatccctc actttgtcct atatttgaaa ttacgatagc
    61741 ataaagaaat tgtttttgtc aactacttcg ggagaatgtt gaaaaatctg ctgagtgtct
    61801 gtcctaagat ttctgttttt tctaatgttt gcctgtgttc cattgcttcc tctaaatcga
    61861 ttggtcctaa ttgaatgctt aagacaatgg ggagaaaatc aaagagtcct tccagctctc
    61921 ggtctctact tccgcaaata gcgattattg gttttcgata tttttttgca agcactgcta
    61981 cgccatatgg aacttttccg tggattgttt gctgatcaat ccgcccttct cctgtaatga
    62041 tgacatccgc ttgttcgatc tgtttttcta atccagtgat ttggctgacc aactcaaaac
    62101 cagagaccat cttcgctcct aacaaaaaaa gggctccgcc aataccgcct gctgctccgc
    62161 ttcctgggat gtcatctaaa tccacctccc aaacatcttt tatctgctga gcaatctttt
    62221 cagcttgttt atccaagaaa gctaacgtcg tatccgttcc tcctttttgt cttccaaatt
    62281 gccaagcagc tccttgttga cctgtatatg ggttggtcac gtctgcagcg atgatcactt
    62341 ttactttaga aacaagaacg ttccttcgat ccaaatgaag ttttctagta gataacaaag
    62401 gatttccttc tgtgtaccct tctaaagaag cacccaaaga ttggagtaat cctagaccac
    62461 catcgctaca tccgcttcct cctaaagtga caatgatttg ttccacttct tgaagcagtg
    62521 tatctttgat aagctctccc aaggcataac tagaagcaag tcggatggtt tgatccgttg
    62581 gtttatcaag taaatctaat cctaatactt gtgcagattc aatgactgcg gtattttttc
    62641 cttgccagtt cgtcaataaa taactgattt ttcgctgacg aaataagaga tccatcccat
    62701 caatcgtacg ccaggttccc ccgagcgcgt aatagattgc ttctgcagta ttttctccgc
    62761 catctgcaat cggcacattg atttttcttc ctttacggtc ttcccaacca tttagaactg
    62821 cctgatttaa ttcaaatgaa gaagcacttc ctttaaatga atcgattgcc gccaaaatat
    62881 ccatctatat ccctcacttt cttcttaagt aagacacttt ttttcacttt tttcaaacaa
    62941 aacaatgcta ttttcttctg tttttctata tactaaaagg gaactcattg aataaaggag
    63001 agaaaaaaca tgaaaatcgc aatagctgga gccggagcaa tgggcagccg aatcggatta
    63061 atgttgcatc aaagcggaaa tgaggtcgtc ttgatcgatc gctggcctgc tcacattgaa
    63121 gccattcgta caaatggatt gattgctgac tttaatggca aagaagtagt tgcaaaactg
    63181 ccaatctatt caccagaaga aatcatcgaa agcaacaaac acgtggattt gatcgtagcc
    63241 ttgacaaaag cgaatcaatt agatgatatg tttcgttcga tccaatccat tattactgag
    63301 aatacttatg ttttgtgttt actgaatgga ttaggtcatg aagatgtact ggaaaaatat
    63361 gtaccaaaga aaaatatctt atttgggatc acgatgtgga cagctggtct agctggtccc
    63421 ggaaaagtca cattattagg tgatggagaa atcgaactag agaacttgga accagaaggc
    63481 gaagcattta caaaaaaagt tgtagaagtc tttcaagaag caaaattgaa tccaatctat
    63541 agtcataatg ttcgttattc catttggcga aaagcttgtg taaacggaac attaaatgga
    63601 ttatgcacga tattggattg taatattgct gaattaggtg cgcaaaaagc ggcagagggc
    63661 atggttcgaa cgattgtatc tgagtttgct tctatcgcag caaaagaagg aatcacatta
    63721 gatcaagaag aagtgtacca acatattgct tctacttatg atcctgacaa catcgggttg
    63781 cattacccat ctatgtacca agacctgatc aaaaaccatc gtttgactga aatcgactac
    63841 atcaatggtg ccgtttggcg aaaaggacaa aaatatgata ttgctacacc atattgtgcc
    63901 tttttgaccc aattagtcca tgcaaaagaa ggaatccttg gcgcagaata aaaggctctc
    63961 tgatgagaat cagctggatc agcagctggt tctcattttt ttacccccat gaaagttcaa
    64021 aaagaaaaac acaattctca tgttgccata agaattgcgc ttgtttattt tcatctatga
    64081 ttggttggca cttttattta tttttaacag aggaaagatc gtattcatat tgttcataaa
    64141 tagcggagtc agtagtgaaa aaacgttttg cccaattttc tgcatcaaaa aattccaaca
    64201 gttctgatgg gattggttga aatggcgtat gcaaaaattc cggtaattca tctaaatcac
    64261 gttcttgaag caaaaaaata ttttcagaat gataaaactg ataatgagct atggcttgat
    64321 tatctgtcac tagtttcttt tgaaaataca tagattcaaa cgttcgcaaa gtcactcctt
    64381 gctgcccttc ccgcaacagt tctagaatcc ccctcgattc cccggttttt ttcagatatt
    64441 cttcataagt aaggtatcca ggttgttcat ttcctcgaag cggaagaata aagtaattgg
    64501 tcgtaacacc tctttttgta aattcctgct tatatttttc tgcccgttct tttctgccgt
    64561 tatttgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa tccatatgtt
    64621 tcgaataaaa ggaactatta tgttttagtc caagttcttt cgcttctaaa aaatcaaaat
    64681 gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg
    64741 ctttaaagtg gttccaaaaa aataacacga tttttgcatt ggtaaatgga cgcacgcgtc
    64801 gagccatctc catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact
    64861 gttgtcgagc tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta
    64921 aagagcggat ggaaggaaaa aaataaagtg atttattttt cgcaattagc aatacgtcgt
    64981 ttttgttcat ctgttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa
    65041 atagtaaaaa gccagtaaaa cgcttcctat tatttttata aaaatttaac atttctatga
    65101 aaatacgatg aaataaatca ttcttccatt aaaatatgcc caaaagtttt ctgaatagtt
    65161 gtaattatct gaaaatttgt tatcattagt cattattatt attactattt attcttacta
    65221 tttattctta ctatgcattt tgattgtgaa tttaaaagaa agaggttatt tatggaagat
    65281 aaaaaattga aaaaacaact tagctcccga catatcacca tgctagcttt aggtggagcg
    65341 attggtgcag gtctatttaa aggaagtggc gaagcgattg gaatcgccgg cccttctgta
    65401 ttgatcgctt ttttgattgg cggcataatt ttatatatcg tgatgaaagg tctgggaaaa
    65461 attgttttaa gtggtggtga tacgcaccac ggattatctg gattgattcg tccatatcta
    65521 ggtgctcatg ctgccgattt taccgattgg gtctactggt ccatgtggat tatcaatatc
    65581 attgctgaag ctgttgcagc tgcttccttt ttacagttgt ggttccccca catcccagct
    65641 tgggtatttg tttttatgtt ggctgtttta actactatca tcaatcttta ttcggtacga
    65701 ttattcgcag agacagaata ttggctggcc tttgctaaaa tcagtgtcat tatcctattg
    65761 atcatttttg ctgcttatct tgtcggtcag caaatgttgg gtactggtgt tttccctacc
    65821 cttcaacaat taactgatca tggtggtttt actccccacg gtatgaaggg aatcatcagt
    65881 tctttgcttg tagttatcta ttcttacggc ggttctgagt tgattgctat cacagtaagc
    65941 gaagcagatg atcctaaacg tgcgattcct aaagcaatca aaggcgtcat gggaagaatc
    66001 atttcctttt acattattcc tttatttttg cttttgatta tttttccttg gaatacttta
    66061 gcaggcacga acgttagtcc ttttgtgatg gtatttgaaa aaatgaatat tccatttgcg
    66121 gcagatatcg tcaatttcgt gattgttctt gctctgttct cgtctatcaa ttcaggtgta
    66181 tatgcttctt cgagaatttt gttcttccgc ttgaaagatc ggaaaggttc ctctagaaaa
    66241 ctagctgttt taaacaagca tcaagtaccg caacgtgctg tgctgttttg taccagtgtt
    66301 ctttatctag gtgtggctct ttcttatttt gtcggtgaca aattatttgg ctatctggca
    66361 ggttctcttt cctatacagt attattgatc tggatcatta tcagtgctgc gtcctttgta
    66421 ctgacactaa ggaaaggaac gttatgggac aaaggaatca gcttttttgc attagcagtc
    66481 ttatggttga ttttccttgg tatcctattc acgaactcca ttggggtaac tgtattgacc
    66541 ggtttgcttt acctgttcat ttatttcagt tatcaaaaaa agaatgatgc tttcgtcttg
    66601 acgaatgaat gaaaccaata gagaatttta ttctatttat attgaatatc tggagctatt
    66661 gaaaaaaagg agctgcgaca aaaattgttg cagttctttt ttccgaataa acggtgttca
    66721 aaaaccatct gtgcagatta attgattgtt ctccacgctc atgacatgat agaagaaagg
    66781 aatgaagaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgattg
    66841 gtgtttcgac ggtagtcatg tttggtatga tgtatttgaa tgtttatgcg cttgatcata
    66901 tcttcttcag tcgcacacgt gtattcatgg cgttgatgat gggtgcagtc atggcagtca
    66961 tcatgttgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgct atgattttag
    67021 ccgctagtct tcttgttttt gccggttcct tatttatggt acgaagccaa acaactgtca
    67081 acgatacggc gtggatgaag gcgatgatcc ctcatcactc tattgctatc ttgacaagta
    67141 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
    67201 aagaagaaga aatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatcca
    67261 tccaaataaa aaaagaggat gttccagaag tgatcaactt caagaaataa gaagaagcta
    67321 cttctggaac accgtgaatc agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    67381 cagccaaaca aaaatagctt ctcatatttt tcgtttggtt aggcttatta ccgcaaatgc
    67441 aagcttttga acaccgttta ttcgtaaaag ggagtgtgac aaaagtcttg tcacactccc
    67501 tttttacgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg cggtttcaca
    67561 aatttctttg agatccccca acctgtaaag gcactgacca tcgaaaacac aggtgaaagc
    67621 aaactgaaaa acacgaatgg caagtaagtg agtgtaggaa tgccaaatgt acttgctacg
    67681 aaacttccgg cgattcccca aggcactaag tagttgatta ctgttccacc atcttctagt
    67741 gttctcccta atacagttgg atcaaatcct gctttttcgt aagtttcttt aaatgcatta
    67801 ccaggcagga taacagatag aaattgttct ccaataaaga tatttactcc gatgcttgtc
    67861 atcaatgttg taaaaatcaa ttttggggtg ttcgtcatct ttcttgatac tcgttccata
    67921 acggttgaga tcaacccaaa ttcgatcaac aaaccaccta atgacaatgt tagaataatc
    67981 aatgacacag ttggcatcat gctttcgatt cctccacggc taagtagtga atcgatttgt
    68041 tggtttccag tatttgaaac aaatcccgat tcgatgaccc ctgccaactt cgttaaggaa
    68101 gtttgtgggt tttgaataaa gatcattcct actgctacag caatattcaa caaaatcgtc
    68161 aaaattgccg gtaccttttt ccatgcacac aagagcatta aaaatagagg aatcagtgac
    68221 cagatcgaaa tagtaaagtt tgcttctaaa atatcagtca cttcacgaat agatgaaaga
    68281 cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc
    68341 ggaacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc aacaatagct
    68401 gctgctaaat ttgttgattc ggataatgga gacatcttgt ctccaaaaac ggcacctgaa
    68461 acaatggctc caacaacaag ggctggattt agtcctagtg ttgtcccaat accgaaaaat
    68521 gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc tacgattgca
    68581 caaacaacaa atacagaagg gacgaaccac tggacactaa tcaatttaaa tccaatgacc
    68641 atgagggttg gaataattcc tgcttgtatc catacagcaa tcaatgaacc gactaaaata
    68701 aaaataaaga ttggaatgat ccccggtttg atgccattga tgatcccttc gtgcagccaa
    68761 tctgtcgggt atttttttat cactgcgtac aacatgacca ttccgatagc tgccaaaatc
    68821 gtgatattag gtgaaacctt taatccgaca acgccaatcg caatgcctaa aacaatgacc
    68881 atcaatacaa gtgttgcttc taaaaaactt ggtgttcctt ttttttccat gataatctct
    68941 ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact ccgccacaat
    69001 tcatccgcga ttcctccttt ataaaaataa aaagacctgc tttcaactaa ggacgaatga
    69061 ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac gattattcaa
    69121 atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag cgaccaccga
    69181 ctttctgaac aaaacaccta accactactg cgtgaaaagt tgacttgtat gaagtttacc
    69241 ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct gaaaattatt
    69301 tttataactt tcgcgcacaa ctttatcttt ttactagaaa gtagcgccct cttgtttttt
    69361 tcttgctact atttcttgat gttcttgttt gattttttct aatagcttgg gttctctcaa
    69421 taaatctaga gctgtattgg caagtaattc cgctgcaatc gcaatagaag caagtccttt
    69481 ttcactttta gcagcagctt tgaattcttc tgtatgtccg gcaatatatg tctcagagat
    69541 tgctacagta ggctgtatcg ttggaataac ttggctaaca tttcctacat cagaagaacc
    69601 tagacttgtt ctttgttccg tttctatttc ttcatcagga acccctgctt cttttgcatg
    69661 ttggaagaat aagtcatcga aagaaggcgt tacgatgaca tcatcgactg cattttggaa
    69721 caatccaaac tcaaaagtcg tacccgtgct taatgcagca cctcgtacga tattttccac
    69781 tttttcatat actttgtcta atgtttgtcg atttcctgca cgtaaataga aacgtccagc
    69841 agcgtagtct ggtatgacat tggccgccac gcctccatct gtaatgattc catggatatt
    69901 gacatctttt ggcaattgca agcgtagagc gttgattccg ttaaacactt ggatcaaggc
    69961 ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgagaagcga caccgtaaaa
    70021 tttgatatcg actggatcat ttgctaaaga ttctgtcgtt ttcccataac gaaaagctgg
    70081 atggacacat aatgctgcat cgacatcctc aaaaaaacct tcccgaacga agcttccctt
    70141 agctgatcca ttttcgcctc cttcttcccc cggagtacca tacacacgaa tttctccgcc
    70201 aatctcatca atgaattgtt tcactatgct ggctgctaat acggagtaag taccaaataa
    70261 attatgtccg catgcatgac caatacctgg taaagcatca aattctgcca aaaaggcaat
    70321 tgtcggacct ggtttattgg aaatataacg tgcatcaaag cctgttcggt gcccagctac
    70381 ttctttttct acccagaacc cttctttttc caattgtttg attaaagctg ctgaggaata
    70441 aaattcataa ttacttactt caggatggtc gtggatagct agtgcaagtg cttgatattc
    70501 tggtaaacgg tattgaatca gatccttcaa ttgatttgtt gagagtgtgg ttgtcataaa
    70561 aatttcctcc tatgctagtc gttttttctt attttctccc gaatgtttcc cacgcaggta
    70621 cgcttgaacc ttttgatgtt ttttcgatta ctttggctgt ttcttctgtt tgataggcat
    70681 ctactatttt ttgatacgtt ttattgtctt catcttcttt tcttgcaacg atgatattga
    70741 cataaggacg tgcatgctca tcgactggtt ctaagaagat tgcatcagaa gcgggattaa
    70801 atccagcatc tacagctaca ccgctattga tgactgaaat atctacatct gacaaagcac
    70861 gtgctgtttg ggaagcatct aactcagaaa tgtctagatt ttttttattc tcggtaatat
    70921 cagaaacggt aggcgcttgg ccttttgcct tgtccacttt gatcaatcca gcagtttgca
    70981 ataacagaag agctctgccg ccattcgtga catcattagg gattgcaact tttgctccat
    71041 ctttgatatc agccacatct tttaacgtat tggaataaat tcccaacggt gcattaaccg
    71101 tattcccaat cgatactaaa tcagttccgt gttcttcatt gtaattgtct aaaaagaatt
    71161 gatgttggaa ggaattcaaa tcgatcgatc catctgctaa agccgcattt ggctgattgt
    71221 aatctgtaaa tttcacatat tccaaatgga tgttttcctt tttcaaacgg tctttcacat
    71281 cgtcccatac atctgtatct tccccaatga tccctagttt tacggtcgtt tctttttttt
    71341 cagttgtagc tgcttttcct tggctgcatc ctgccaacca aataattgct acaaatgtaa
    71401 tgactgcaaa acgaataatt tttttcattt tttctcctcc attttcaatg tgtgactttt
    71461 ttaatcagtt gattactgat aaattgactc aaaaaaacta agaccagaat caataatgta
    71521 gcgacaatcg ttacatctgt ttggaatcga ttatatcctc tcgatatagc aagattccct
    71581 aatcctccgg cacctactgc tccagccatg gtagtcagtc cgatcacatt gatgatcgtc
    71641 aacgctgaga cccgaatgat accaggtaac ccttctaaca aataaacacg taaaatgatg
    71701 ccgattgggc tcgtgcccat cgcttctgct gcttcgatca ccccaggatc aacttctagc
    71761 agtgcattct cgatttgacg ggcaaaaaac gggacaacgc caaaaatcaa agggactaaa
    71821 gctgctttgg caccaatcgt cgttccaact aagaaacgag tagtcaaagc taaaagagaa
    71881 agcaagataa tgaaagggat ggaacgaatg atattgatca acttatcaag tatttcgaac
    71941 agcagcctgt tagctagtaa tccatttgga cgtgtcacta ctaacgtcac acctaagatg
    72001 attccgatga tcccaccaat gaatgccgtc caaaaagaca tatatagtgt ttgctttgtc
    72061 gcttcgatga attcatccgg aatcaaatag acattcggca aatatgtttt aaaaaattca
    72121 gtcattttag tttcctcctt cgatgacttt gatacggtta tctaattcat ttttttgtaa
    72181 aatagttact tttacaccag atttaatcaa ataatccaat gctttttgcc gctgtttggt
    72241 ttctccagct aaagtaacga tcaaacttcc taagggtgtt tcttgtaata gctctacatt
    72301 accgtataag atattcgctg ttacttggta cttgctgtaa agatgagcga ttagaggttc
    72361 gttcgtctgg ttgccgatat aacttaattc caccaaccat ttatttgcaa tctgatcggc
    72421 aaaagcagca tgggcactga ttgtttccaa tgcttggtca aggtgtgtcg ctgtccgaat
    72481 aaaatcttta gtcaattctt cttcaggatg actgaaaatc tggatgctgc tacctttttc
    72541 aatgattttc cccgcttcca taaccgctac cttgttgcag atttctttga caacttgcat
    72601 ctcatgagtg atcaggacga ttgttagacc tagctgacga tttagttttt ttaacagtgc
    72661 aaggatctga agggtcgttt tcggatcaag tgcactagtt gcttcatcgc ataacaacac
    72721 tttaggttca tttgccagag ctcgtgcgat tgccactcgt tgtttttgcc cccccgacag
    72781 ttgtttagga tacgcagatg ctttttcttc cagaccgaca agttccagta gttcattcac
    72841 tttttgacga cgttcctgtt ttgattttcc agaatacttt agagaaaaat ctacgttgtc
    72901 aaaaatcgtt cgactttcca ttagattgaa atgctgaaag atcataccga tggcctttct
    72961 ttcttttcgc aattcttttg gagagagctg gcttaaattc gtctgattga cgatgacttc
    73021 tcctgctgtt ggtttctgca acaaattcat cacgcggact aacgtactct ttcctgcacc
    73081 tgagtaaccg acgatcccat aaacatcccc tttttcaatc gttaaatcta cagaatctac
    73141 tgcctgtatc tctttttctt tttgtttgaa acatacagaa atatctcgca attcaatcat
    73201 taacattcct ccttgtctat aaaaaaagca tcctcaagaa aatgaaaata gtcattttct
    73261 tgaggacgcc tttttgcgtg ttaccacctc atgttcaagc tgtgttcaca cacaactcct
    73321 cttcaagtac catcatactc tagcaagata tcggtcgcag ccgtcaccgc cttacatcta
    73381 tgctcgggat gaattgctcg atggccattt tccaatagtc gttccttgct tcttctcagc
    73441 taccgaagct ctctgtcaaa ggttcttcta tcgtacttac catttcaatg caaataatat
    73501 tcaaaaaaaa aatcctttta ctaacataaa ctatgtcagt aaaaggacga cgtatcgtgg
    73561 taccaccttt tttcggaagt ttccttccct caagcccaac atctcaaaaa gagagattgg
    73621 cgttcgtttt aacggtcgaa catttccgaa tttgcctact tatcgacaaa ttattctcca
    73681 aggccatttt cagcttttct tacttgactc ttcacaccag ccagagcctc tctgcaaagt
    73741 ttccaagctt actttccttc tcaacgaaat caatattttg ttgttgggaa taatctacag
    73801 gaggaaaact gaaaagtcaa gcatgaaatc caaattattt tttctatatt tatttttctg
    73861 tttttttatc taacatttca ataacagttc gaagatcttt tccttccgca agaaggttca
    73921 atttttcctt ctcttcgacg ctataatttc gaacaaaact ggaatcggga atcaaaacaa
    73981 cgcctagtcc tgcacgtgaa gcggctgtcg ctcctactaa agaatcttcc actgcaaaag
    74041 ctgaaagagc ttctaatcct gttcgttcca aagctgtcag ataagggact ggtgaaggct
    74101 tgttctccga tacttcatct ccgaacgtaa acgtatcaaa gtaatcggct aattgaaagt
    74161 gatccaagat atctgttgct cttttcgtaa cagttgtcgt agcaatccct agtttcatat
    74221 gacgttcttt taagtctgtc aatatttctt ttgcataggg tttcaattgg atagctccac
    74281 ttgtcagctg ttgataaaaa tattcttcac gctttgctcg gacttcctgc actttttcag
    74341 gactgcctgc tgcctgcact aaaatatcaa aactttgttt ccagtttttt ccgccccaag
    74401 cgtcaacgat ttctttcggg atatgaatat caaattgctg gaatccccaa agccaaccat
    74461 cccgatacgt tctttccgta tccactaaca aaccatctaa atcaaatatt gcacctttca
    74521 tctatattcc aaaacaatac acctagttta gcatgactga tccaaaatga acagaagcat
    74581 agagcccagc cgcatcactt ataagagttc ttggaatcga cggatataga ttttcttcac
    74641 agctgtcaca agcgccagat aagcgacgac tgttataaac aaccataacc agtaattgcc
    74701 agggagcggc atcaagccta actcagcacc aaatcctgtg aatggaagga ctgaaccgac
    74761 aatgattcct aatgtagtga taaccagcat ggcaaatgtt gcattgcttt ggataaatgg
    74821 tattttcggt gtacgcaatg catggagtac taatgtttgt gtccacaggg attcgacaaa
    74881 ccatcctgca tggaataatg cgataaaaat cacttgttgt gagctatcca atgtatggaa
    74941 acttccacca actacagcag gacaaataac aaaatacatc aacaagtaag tagtgatatc
    75001 aaaaatcgaa cttgttggac caaaataaac catgaatttt ccaatactag atgcattcca
    75061 tttctttggc tcatccaaat attctttatc catccgatcc catggtaagg aaatgcaaga
    75121 aacgtcatag atcagattca aaaatagaag ttgcaaaggc aacattggta aaaatggtaa
    75181 gaacgtactg gcaatcagta cggaaaacat attgccaaaa ttagaacttg ctgttgcctt
    75241 gatatatttc atgatgtttc caaaagtttc tcttccagaa agaaggcctc tttccaaaat
    75301 cgtcaaatct ttttctaaaa ggatcacgtc tgctgattct tttgcgatat ccacagctgt
    75361 atccacggaa ataccgatat cagccacttt cattgctgga gcatcattga taccatctcc
    75421 taaaaatccg acagtatgcc catttttacg taaagctgtc gtcaatcgtg ctttttgctg
    75481 aggattcaat tttacgaata cttcgtgttc ttcagctact tgcgctaatt catgatcatt
    75541 catttccgag attttctcac cagtaatcaa ttcattgatt tctaggccta cttgacggca
    75601 cacagaccgt gtgactaatt cattgtcccc agtcagaact tttacagcta caccatgttc
    75661 ttttaaagct ttcaacgctt gttttgttgt ttcttttggt ggatcaagga aagcaagata
    75721 accaatcaga accatgtctg actcatcttt gactgaaaat tctccaacaa cgcttggatt
    75781 ggtcttttgt gcgacaccaa tcactcgtaa gccgtcttca ttaagtgatt gtacttttgc
    75841 aagcaccgct tcttttcgtt cttttgttaa tggtgcgaca gtaccaccaa gatcaataaa
    75901 tttagaaatg ttcaacattt cttcaatcgc gccttttgtg atcatctgtg ttttgccagc
    75961 cggatcagcg actacaacac tcatacggcg acgttcaaaa tcaaaaggaa tttcatctac
    76021 tttttgataa ttgattgtat ctgtatctaa agtttctgct gcttcatcaa tgatagccac
    76081 atctaacaaa tttttcagcc ctgtttgata gtagctgttt agataagcat ggcgcaaaac
    76141 acgatcatct tcatttcctt cacaatccat atgatattca agaatgattt tgtcttgtgt
    76201 caatgttccc gttttatccg tacataacac atcgatcgca ccaaaattct ggatagcatt
    76261 taaatttttg atgactgttc cttttttggc catcacactt gctcctttga ccaaattcgt
    76321 cgtaacgatc attggaagca tttcaggcgt taaaccaact gccacagata atccaaaaag
    76381 aaaagcttct aaccaatctc cttttgtaag cccattgata acgataactg ctggagccat
    76441 caatgacata aatttgatca gcagcatgga tgttttgtgg atgcctaatt caaaacttga
    76501 ttggatgggt ttttcagata gcgtttctgc tacatgccca aatagtgtgt catttccgac
    76561 agaaacaatg attccttccg cgcttccact gatgacattg cttcccataa agacaagatt
    76621 ttcataactt gtttcacttt gctgatcgtc ttcacatact ttcgcatgct tttctactgg
    76681 atagctttcc cctgtcaatg ctgcttgaga aatgaataga tcctttgcgg acaataaacg
    76741 catatccgct ggtatcatat ctcctgctga aagccgaaca agatctccac agacgatttc
    76801 ttccatagga atctctgtat actcgccatt tcgtttgaca gctgccgtta ctttcacaag
    76861 tgatttcagt ttttcagctg cttgattaga acgcacagac tggatcaacg tcatcgtccc
    76921 actgacgata accatagtga agataatggc tacaccaaag agatctttat ctcctggagc
    76981 ggctaatacg tattccgtaa tgaatgaaat aattcctaat gcaatcaaca ctaatgtaaa
    77041 gggtgtgatg tatgctttca atatttctat aatgaatggt gtttttttgc catatgaaat
    77101 cgcattttca ccataagttt ctcttgaacg ctcgatttgt tcttctgaca atccatttgg
    77161 tgatgcatgg aacaattcaa ataattcttg cttcgttttt ccagcaaaag ctgcatagat
    77221 tttttgtgta tctttctttt tttccatctc gatctctcct ttgtgtccaa aagacagctg
    77281 ttctcgctgt ttttggtctt ttgctattca tcggatcgat agagaaaatg ctgaaagggg
    77341 ctgagaacag atagataata gccctggcct ttcctctaaa tccttgtttt tcgaatgact
    77401 gtcatccatt tgttctcatc tcctttacta agtaatttta attcatgact tccaatcatt
    77461 tttcgaatag ttattttccc aactcgctca cctccctttt tgttttttca cctatgggat
    77521 ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcaaatca
    77581 aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata
    77641 tctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc
    77701 gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatcc
    77761 tattcggttt tccctcgtta atataacaac taaaaaagag gatgtcaaat caattttcca
    77821 tgtttttcat acttttttaa aatacacgcg ttatatttcc tgtctcacgc agttttattt
    77881 gtctcatttt ccacctcccg ctataataga attatagtag gaaacgaggt gctgttatgg
    77941 aacgaacact tgttattatc aagccagacg gcgtaagacg ccatttggtc ggaagcatca
    78001 tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaaatttgca acgatgacac
    78061 ctgaactagc aaaagaacat tatcagcact tgactgaacg tagttttttt gatgagctta
    78121 tcgattacat gacttctggt ccggtcgttt atcttgtgct ggtcggcgag gaagtgatcg
    78181 atatcgtccg taagatggtc ggagcaacaa aaggggcgga tgccgttcca ggtacgatcc
    78241 gaggagatta tgcactgccg gggactgaaa acatcatcca tgcttctgat tcccgtgatg
    78301 ctgcaacaaa agagatcgcc cgctttttcc cgccattcga tacaaacaaa ccgtctgaaa
    78361 aagcggcgaa gtgatacaaa tcctaaactg ttggtctaac tgctgggttt agcaatggcc
    78421 agcagttttt tctctaaaga attcctttga ttcttcccta atgtatattc gatcactgcg
    78481 aaaaactctt ggtgattttc cgatttttct tcccctaaag cagcagaaat cagtcgttgg
    78541 taataatccg ccatcaatgt ttcataaatc actgtacttc tttctttatg cgctaaactc
    78601 cgtttcatat cttcattcac gctacgtgcc ttttttaagc gaccattttc tatataaaac
    78661 aaagtcaagt tcgtcaaaaa agtaaagaga tactgctcgt gattattcag ttgcgcgatc
    78721 cgtcgcattc tttttttgat tttcaaataa ttattctcaa taaaacgatc actgaatatc
    78781 caaggaaaat ctgctagaat agatacttca aattgcccat actctggtac ttgcatcaaa
    78841 tattcttcaa tgatacgaca ttcataacgc aaaaattcaa attccgtctc agatacttct
    78901 gtttttttgg acatgtgcag attcaaaacc aaatagtaca gatcttctgt tttcttatag
    78961 tgcctgcggt tattagcaat ttctttgtaa ggatcaaagc tgtcttcttg aatccgatct
    79021 agcattcgtc tttttttctt taattttttt ggctcatcaa gctgatttcg tatccattca
    79081 tattcggcaa gtgtcatgtt cattcgctct aaaaatgatt tcaatgttcc aaacgatact
    79141 tttttcactg aatttgtctc gatcccgact agcgaagttt tagaacagat tccttccgct
    79201 aattgggtct gtgtcagctt tttgttgact cgaagcttct taatcaattc tccatcatac
    79261 ataatagttc tcctttttta attgaaatta acccttggct tttccagaat gcttgccaat
    79321 aagaggaatg attatttcaa tttaaatttt ccgcattcaa taaatagata cgttaaaaat
    79381 aaaaaataca cgaaaaaaat aaaaaaatgt ttttctcata aaaaatacca accttcaagc
    79441 atgagactgc tgtctaatcc ctgaaggttg gtttgattgt ttattgatca tttttttact
    79501 gctccgcatt ggttcctgtt actggatata ggatagtcgt tgttttattg ccgttttttg
    79561 aaaagacact ggtcgaatgt tcaccttttt gcttttcgat tgcttcatta cgttccgctt
    79621 cgtttttata gtcatatttt ttcggatcaa tcgtctcaaa tccgtttggc acataaaacc
    79681 gcaagagatt ttcctgattc agcttatctg aaagacttaa cgcagtcttg actttttcct
    79741 gatctttgga gatttcctcg tcgattcctg ctgctttcgt atcgatgatt tctcctgtct
    79801 gattattgta agcttttcct cctaggacgg tgtatttggg cgtcacgaag ttcccatttc
    79861 gaaaagcaac tacttgatcg tgctgagaag ataataagtc tgtaccaaaa tgaagataat
    79921 ctttatcatc gattcccaat agatgtagta atgtcggcaa aacatcgatt tctccaccat
    79981 attcatgatt gactgtccct ttagtgtaac caggcatatg gatcatcaat ggcactcgtt
    80041 gcatctgtgc gttgtcaaat tcatcccatg tatctggatc tttaccaaga gcacttgcta
    80101 aatctttatt atctgtattc gagatcccaa aatgatcccc gtaaatcaca aagattgaat
    80161 tttggtaaat accagatttt ttcaaatacg taaaaaattg ttccaatgat tgatccagat
    80221 aatgggccgt tcgaacataa ttatctactg tgctattgcc agtattcaaa gatggaaagt
    80281 cgaaattctt gtcatccgta taataaggcg tgtggttggt tacggaaaga aatttcgtgt
    80341 aaaacggctg ttgtagatgt tcaagatatt gggcagattc tctgaataaa tctttatcta
    80401 atatcccata acctaacgtt tcatcggatt catcaaaata agatcgatca aagaaattgt
    80461 cataccctaa atttttatat acatgatctc tattccagaa actagctaca ttcccatgga
    80521 aaacagcact tgtatagcct tctgtttgac caagggtcgc tggtgctgct tgaaagacat
    80581 tatctgaccc aagttctgtg aataatgagc cttgaggtaa accaaatgtg ccagtttcca
    80641 acatgttttc agcatcactt gtctttccct gtcctacttc atgaaagaaa ttgtcaaaac
    80701 tgatcgttgc ttgattctgg aaaatactat ttaaaaacgg cgtgacttct tgtccgtcta
    80761 ccttcatatt gatcaaaaac tgttgaaaac tttctaaatg aaggacaatc acattttttc
    80821 ctttagcaat cccaaatgtt tcaggatttg gctctgcata atgtttcttt gtatagtcta
    80881 aaacttcatc aattccatta ctgcttgcat gcgcgcgaac actgctagtc attcctgttt
    80941 tgattccgtc ataaatagtg tacgcatcga ttcctagata tttgacaata taggaacggt
    81001 caaatgtccg ctgtagcaat tggggacggt tggcttcgct caacgctaaa tttcctgaaa
    81061 aaatcaatcc tgaaagacag gtaacagcga tagccatcgg tttgctgaca gggttgcttt
    81121 tgataggtat tttcttgacc ttcaaccaaa ccagtagccc aatgaacaca gcgatatcca
    81181 accaatagat gacatcatgg ggtttcatca aagaaaagga gcttcctgac aacccttgtg
    81241 acactttcga aaagcctaat acagatttga ctgtaatgaa atctgtgaat tcccgataaa
    81301 aaataatatt caagtacaac aaaagcgtat ttaaaatatc aatgatcaaa agaatggcta
    81361 acgctggctt tggcttttta atatacaaag aaaggccaaa aaacaataag gtagtcgcaa
    81421 tcgggttgat ccacaagata aagtactgga tcgtaccttc cacacctaat gaaaaatctg
    81481 catagtaggc aatgatcgtc ttcagccaga aaaagaaaac agctgtcaaa acgattgcta
    81541 aacgattttg gcacaatttt ttaagttgtg tcacgtatat cacttctctc tattcacatt
    81601 ttttcttatt ttgctcctga aaaatgaaaa tttcctaaaa aagttgttat cttaagattt
    81661 ttttattctt tctaaagatt attccattca tttttcacag cataaaatcg tttcggtgta
    81721 agcggttaaa atagtaagga gataaaaatc ttgtggtgac cgatttactc tatagtattc
    81781 aaaagtagtt tctcggtatt cgtacaaaaa aaacatacta agattagagg ggaacatcat
    81841 tgacgaatga tgttctcctc tattttctat ttttagatta tagtgaagag tgaaagaaag
    81901 ttcgaacaaa gccttgagga ctacgagtcc ctatcaaaaa ccgaacttct ttactgtcat
    81961 tttagaatca ggtttacgta tgttggggcc atgagcccgt gtataggaaa ttggaatcga
    82021 aatgaagcag taaagttctt tcaacactta aaaaggagga aaacaaatgc cttacattct
    82081 tgcattagat gtttcaatgg gacacagcta cagtgtccta tacaaagacg acacctgctt
    82141 atttgaagac gagatcgaac acactcaaag aggatttcat gcattactcg aagagatcca
    82201 gcacttgcct gaacgtccat tgattgtttt tgaagcaacg ggtatttatt ccagaccagt
    82261 cgaaaagttc tttcaagatc atcacttttc ttactgtttg ttaaacccgt tagaagcgaa
    82321 aaaacagatg gaagaaaaca ccttacgcag ttggaaaaca gataaatcgg atgcccatcg
    82381 gctcgcacag actcatctta aaaatcaacg tcaaccaaaa gaagtacaaa gcaattttta
    82441 cttagagatg cgcgatttag cccgttttta tcaagaaatt gagaagaaaa tcactcgact
    82501 gcgcatggat ttacacaact gccttcagtt gacttttcca gagcttgaac agttcttttc
    82561 aaacaggctg accccttacg cattgacatt gatccgtctt tttccccatc cagattttgt
    82621 attggcatct actcggacaa aaataaaaaa taaactgatc aacgaaacac gggaaaaaat
    82681 ctccgcgaat cgtgccgagc aaaaggcaga tcaaatcatc cactatgcac agtgtgccta
    82741 tccagctgtt gaaaaagaca gtattcactg tcaaaaaacg atctattacg ccgaacttct
    82801 ccaagattta ctcgaacaaa aaaaagcact ggcgacacaa atgatcaaaa aagccgaagc
    82861 ctctccttgc tttttgttgt accaaacgtt ccctgggatt ggcgcactca cagccgcttt
    82921 attgctcggt gaattaggag atatcacacg atttaaaacc cataaacaat tgaatgcctt
    82981 tgtcggaatc gacatccgac ggtatcattc aggtaagtat actggccaag accgcatcaa
    83041 taagcgtggt aatcccaaag cacggaaaat tattttcttc accattcgaa atatgattcg
    83101 ccagcaacgc gcagctccta atcatattgt cgattattac tacaaattaa aaaagcaacc
    83161 tatccctaag aaagataagg ttgccacagt tgcttgtatg aacaagcttc tgaaatgtat
    83221 gcacgctatg gttagggcac atacagagta cgactatgcg tacgcggtct cagtggacca
    83281 ttaactaaac cacttacagt ataccacttc tttgcccaga tcgaaaattt tttacgaacg
    83341 gggctttatt tagtgtgcct tgattttgtc gctttcgttt acgagctatt tttcttgcac
    83401 cttttacttg actaatcgta ggaaagaggc tgtgacaaaa ttcttgtcgc agctccctat
    83461 ttcttgatgc aggacagctt tttcacaacc tctccactaa acggtgttca atcagctgac
    83521 cttcggtatt agctcaaaaa acggcaaaat atgaggagct attttcccgt ttttttctct
    83581 aatactcagg tcaaaccgct gatttcacaa cctctgattc acggtgttcc agaagtagct
    83641 tcttcggaaa taagccgaaa ttttacaaaa acttgagaag cacttttcgt aaatttcttc
    83701 ttatttcttg aagctgacca cttctgtcac aacttctgtt tttcattgtc cataggtttg
    83761 atattgtcct tctgatgact cattcgtaat gattgaggct tgattttctt catttcccgt
    83821 tatcgtttgt ccactgccag aatctgaagg gcttagttct gtcgactcgt ttttttgaga
    83881 agaagcagtt gttttcttga cttgttcgtt ggtctttttc cattccgcta gtcgatcggc
    83941 caccactttt ttgtccactt tttcagaata agctgtaacc acttttccat ttttcaaacg
    84001 tgctaccttg tcgccatcat gagtcaaaag caaaacgacc aaatgtttgt ctttgaattg
    84061 atattgtctg agagaaatcg cactaagtac gtcatcgata ttgataaatt gattttcttc
    84121 ttttcctttt ggcaaataga tgtccaattc acctttttcg taattggcag ttaatttata
    84181 ttcctcccac caataagttg agaattcttt tttgaaatta tctactgatt ctttgcttgc
    84241 tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg aaaatgctgc
    84301 ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt
    84361 cctcatgcct tcctccactc ctatttaatt ttgactattc tattatatac aaaaaatgaa
    84421 atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt tcctgaagcg
    84481 gtaagaacgg gaattttcat acattctctc ttggctattc tttaagcttc aaatccattt
    84541 ctgtctgata catctttgaa ccaatgaccc gatttcttga tcgttctttt tccatcttgt
    84601 gctaaatcta ctgaaataaa accgtaacga ttcttatagg cattggtcca cgaccagtta
    84661 tccatgcatg tccacatatg ataacctaaa caattggcac cttcttgaat ggcttgatgg
    84721 acatatttca gatgagattg aacaaattcg atccgataat catcgtggat cataccatct
    84781 tccttgataa atcgtgcttc attttctaca cccatgccgt tttcagaaat aaaacagtcg
    84841 atattcccat aatgatctcg tacattgatc aaaatatcat agattccttt ttcatagatc
    84901 tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc gaagtaatcc
    84961 tcaggcatag gaccgttttt agtatcgatt ggtgtttctt ttgcttttat tcggcgtggt
    85021 tggtaataat taattcccaa aagatctact gtgttttctt taatgatcgt caaatcatct
    85081 ggatgaatct ccggcattag atcgagttct tttattctct caatcaattc actgggaaac
    85141 tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc tattcgtgct
    85201 gctttaacat cttcgggatt tgattcgtct cttggataac tcggtgtcaa atttaagatg
    85261 attccgatca caccgctttg atccatttca tggtaagctt tgatggctaa tgctgaagcc
    85321 aacgcttcat gataaccaac ttggattgcc tttttcatat ctgattcttc cggataatgc
    85381 catcgataca aatagcctgc ctcgactgga acgattggtt cattatgggt aaaccatttt
    85441 tttactttcg agccaaacaa ttgaaaacaa ctttttgcgt atttttcata ggcttcaacc
    85501 gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc gaaatgatac
    85561 agattaacaa agggttcgat accgttttca atcaactcat caaaatagtg atgatagaaa
    85621 cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct ccattggata
    85681 gaggttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc ttgatacttc
    85741 gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg cggttcgttt
    85801 ttgtaccaat aatcaaaaat attctctcct tttccatcgc ctgaaattct tccttctgtt
    85861 tgtggaccac ttgctgcaga gccccaccaa aaattttttg gaaattgata tcttttcatc
    85921 aaagcacctc tttttctttt ttagttgatt ccatgtatga tattttccat cacaatcact
    85981 ttttcatttt cttcctcttc ttttttgatt cgtttcaaca gctgttctgc tgctcgttcc
    86041 ccgattttat ctgttggtgg cgtgatcgtg atttttggaa ggatactcag atcgttttta
    86101 tctaaataat catagtgact gaacgcaata tccttcccga ttctgacacc ttcttgattg
    86161 aaacattcga tggcaccaat cgacatgttg taattgctga caaaaatagc agttgcccct
    86221 ttttctaata atgcttttgc cccgtaatag ccgctttttc tagaatagtc tcctgtaaag
    86281 atctctaaat ggatagacgg atccttttgt accgcttctt tggctccagc taaacgttct
    86341 cttgctacgt aatctgtttg agcggcagca ataaatccta ttgttttatg tccttgtccg
    86401 ccatgatgag caatcacttt tgctacactt tctcgatcat tcactactat tgtatcaata
    86461 cctgctggcc cacccggaca attcaatgcg acaactggga tactcatttt tgtcagcatt
    86521 tcaaatccag accactcttc ttctgataag aagacgatca atccatcgat cgcatgcttc
    86581 atcaggtatt cgattttttg ttcgattttt tctggctgat cggcaaaccc gcttaataat
    86641 aagctatacc cattttcttc agctactctt tcgatccctg aaacaacctc cgaaccaaat
    86701 cggttagata tcccgtttat caacagtcca atagagaaac ttcgattatt cttcaatcct
    86761 ttagcaatga tgttttcttt atagtccaac gctgaaatcg cttctgatat tttttccata
    86821 ttcttttcct tcagctgctg accgtttaaa tagcgggaaa ctgttcccac tgaaacacca
    86881 gccatcttag ccacgtcacg gatcgatgcc atacattttc actcctgcca gttacatttg
    86941 atttcttttt cttgttgttc gaccatgatg accgctttct ttgcataatg ccgcttgtca
    87001 tcaatatcca tataactaaa gtcttcagaa gtgagttcaa aaacaagcag tctttcctct
    87061 tgagaccgca attcatcttt acgaaaagct ttcaataatt tcgttctttg gatgacaacg
    87121 ccgtcaatta atcggacata gacaagagta cttgttttac ctgaaacact tcctgtattt
    87181 ttaactttaa ttgatattat tactttgtca atatccgttt tgacttccat ctgactgtat
    87241 tcaaatgttg tatagcttag accatgtcca aaaggataaa atggacttcc gcttaaatca
    87301 taataattct cttgtttaga agcagcacgc tgatagtaat aaacaggtag ttgccgacta
    87361 tttctaggat aagacaaact caatcgtccg cttgggttat ttcttccaga aatcatctgc
    87421 gcaatggctt ttccaccctc ctgtccagga aaccatgctg ttgcaatggc attcgtaaat
    87481 gactcgattt tcatcaaatc gtagggtctt ccttgcacaa gtatcgtgat gattggcttg
    87541 cctagttgat acaattgttc aatcagctgt tcttggtatc cacctaaaga aagagaagca
    87601 acatctacat tttctccaga atccatgttg atccctttcg atgaaactgc cccgttacga
    87661 agaaattcca tatcaaagtt ccgcgcactt gatccaccta gaactgcaat gatcacatct
    87721 gagttttgag caagggtcac cgcttctaat aatttttctt cctgatcctc ctgatttctt
    87781 acttcacatc cttgtgcata agaaaccttt gattggggaa actctgtttt tatctcttgt
    87841 actatctttt ttttcaaacg gtcttctgtt tgtggtgcag agtagtctcc caacaagtgg
    87901 taaacatcgt gagcatttgg acctataaca gcaatgtttt ttacttgttg atccaacggc
    87961 aatataccgt cattttttag taaagtgata ctttcttttg ctgtctctaa atttaagtgc
    88021 tctgattcct ccatgagatg atcccaatac tcctttggat cgtttgcaaa tggctgttca
    88081 aataaaccta ataaaaattt gacagataaa actcggcgaa ctgcttgatc tagtagcttt
    88141 tcatctacga cttgattatc aatggcttct gcaatcttgg tataggtctc atcccacaaa
    88201 ctgagatcga ttcctgccgc taatgcatat gctgcagcag tctttttatc tgtaaaaaca
    88261 tcggataaac gatccaaagc cacaccgtct gccataacga ttccttgata accgatctct
    88321 tttcttaatg tttgctcaaa aagcgctcga ttgatatgac aaggtatccc gtcgatgtca
    88381 ttataagcag ccatgacacc tactgcattt cttgtactct tcaataatgg tccgtaaata
    88441 tcggagaact ctctgcgtcc aatcgttact gtgcctgaat tatgaccgcc taaagcttct
    88501 ccttgtgcga tacagtgctt taacacaaca ccgatctgat ccttcttctt attgatttcc
    88561 tctactgtct gatcaagaaa agaaactttt tctgaaataa tctctccttg gaaccctcga
    88621 acgaccgctt cagaaaaacg agctgagaga atcggatctt ccccaaagca ctcttctgtc
    88681 cttccccaac gaggatcttt tgccagatcc aaagtcgaca ccagcgctaa atgaacacct
    88741 ttagcagcca gttcttttgc catcaatcga ctcgattgtt ctaataatgc tacattgaaa
    88801 gtatttccac ggccgatatt cgttggataa gaaatcccat ccaagccttg atgaccatgt
    88861 ggacattctt ctacaagcaa aactggaatt ttccatcgcg aatgctgtag tacatattct
    88921 tggactaggt tagccacctt ccagctttct tttgcaggaa tcccattctt ataatctgtt
    88981 ttagaccacg gatcagagcg aaacagaccg tataaggcac ccagaccgcc tccccattta
    89041 acatgttctt ttaacttttc tgttaattcg atcttgtctc cgtttttttc ataacattcc
    89101 caaccataca aatgctggtt tacctgtcct actttttcag caatattcat cctcgaaagt
    89161 aaatcttcta ctctttggcg gatggttaac tttgggtttt gatacgggta atcgtacacc
    89221 ttaatatcct ccttctgcca attttgcctt attagcggct aatacaaatg gcaggaataa
    89281 aaatacatct agaatgataa gtattactgt taagactgct gctcgtatat cgcctcctgt
    89341 agcaataaaa gattgtataa ccggtggagt gatccaaggt gcagcagcaa ctgttttact
    89401 gatgattcca agacttgtgg ctgcgtaggc aataaccaag ttaatagatg gtataatagc
    89461 gcatggaatc atatatattg gattcatgac tactggtaat ccaaacataa ttggttccgt
    89521 aatattaaat aaacaagtag tcagaccaaa tttagcgatt gatcgctgtt cttgacgttt
    89581 agaagcccac aagatagcaa taactaacgg taaaatacac ccgccacctc cgatcaatcc
    89641 aaatgcattg gtaaagggct ccgtaataat attaggaatt tcttttccta ctgaaaaagc
    89701 atccatgttt tcctgaatag actgtaacaa aggtgcttgc cgtataggag ccaaaatact
    89761 cgaaccatgc ataccaaaca cccataagaa atcagagaaa aactgtaaaa ataagatgcc
    89821 tgggacggat aaaacaaaag ctttcaatgg tgtctgaatc gctaattcaa ttaattgcgg
    89881 aacattcaaa ccagtcgtat ggtttaaagt aaaaacgaca actgaaaaaa ataaaatgac
    89941 aactatttct ggtactaaag aattaaatga cgcagctact gccggtggaa ctccttctgg
    90001 catcctgatt ttaaaactct tcattttcgc taatttgata aataattctg tactgacaag
    90061 ggcagcaatc atcgccgttg ctaagccaga cgaagaagtc agactttgta agaaaacccc
    90121 tgtagcttct gctgtatttc cgtccatcaa agtgactgta cttgataaag acatcaaaat
    90181 aaacattacg gctgtactta gaattcctgc atgaacagga ctaaatgatg gatcatcaat
    90241 cacgcctgtt tggatatagt gggttgccaa attccagccg atattgtaac acacgataat
    90301 agctaaaata ctcatcgtac cattattcac gattgaagca agttcagcca atggacttag
    90361 atcaagtatt ctttggactg ttgcatttga aaaaatcaga gcattcagca aaaccatgac
    90421 agaagcagcc attactaacg gcatagtaac gataaaactg tctcgaatgg ccagcaagtg
    90481 tcgctggcta ctcatcttag atacgattgg tagcattttt tcagatagtc tgtctacgac
    90541 tttcattatt tcatctccta gattctaaaa ttgaaccggt tcatttttaa tatagcactt
    90601 tttttaaaga ttgcaacaca tttttttaaa acgctttcta aaggtggtgg attttttatg
    90661 acttttgaaa aattattcta tttaatggta ttttgtgaat tttttctctt ccatccgttt
    90721 gaagttttac tttccaatgg tataatttct ttatcctatc aaatggggga agtattttga
    90781 agaaattaat cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt
    90841 tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg
    90901 aaaaagcaga tatcaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat
    90961 ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac
    91021 aaggagctga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatta
    91081 ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa gcaacagtca
    91141 catttaccaa aatcggtaat taccgtttga aggataagga tggcaacatc tacagtctat
    91201 agtgccatct gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc
    91261 aagatgactg taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat
    91321 aacaacaaaa atatccatac agaatttaat gatcaaggaa gtttgagtat atgcgacaat
    91381 tgccacaaac tagtcaaaga ttcttttaaa tcgtttgaat cttatcctgt caaaaattat
    91441 gtaaaaagca aaagaagata agcaaaatca agcaaaactc actatggctg atttttatga
    91501 acttcaaact cagcttcttc ctttgatcaa gcttcaaaaa ctggaatcag taaaacaaac
    91561 agtggatcat ttcagttcac aaatcaaaga gtaacgtttt atttatttca catctttgaa
    91621 tataaaataa ccaaacgata gaaagactta ttctacatca cgtagtaatt tttctttccc
    91681 gtcgtttggt tttttacttt tagcctaatt cttgaccttt cgcatgctga ttatctgtaa
    91741 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacgaatca
    91801 ccttttttgc atcttctgga ttttctacaa aatcgaatgt atccccatcc acttgtattt
    91861 taggacatac atcaaaatcc tcataccatt gattatagcg ggtgttcagt gtttcgtaat
    91921 aggtgtataa cgtttcatcg ctttctagct gttcatagtc acgcccgcgt tttttgatac
    91981 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcagga cgtttttttg
    92041 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    92101 cgatatccgt tacccgacct aaatcagcat ttagatggaa caacagacta tcttcgtaga
    92161 tggagcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    92221 tattcaaaaa gaagatttgc aataaaaatg cgtactgctc aggatttgaa taaaaaagcg
    92281 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    92341 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    92401 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatttttcaa
    92461 aatcatattc acgtacagct tggtctgatt ttaaatacgt gtgaagactt tgtgtaatca
    92521 tcaagttcgt aggtaccgtc tcttcttgta cgatgatcat cggttttttc aacatagttg
    92581 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    92641 ccaaaacaaa ttctgcattt gtcaactctt ccatatcttt ttcgtatact tcctgcgccc
    92701 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    92761 tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    92821 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    92881 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    92941 gatcacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    93001 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tatcaggtgt
    93061 caggccgatc gctatccaag aaagaagttg cgaaaattcc tccaatgtac ctagacctcc
    93121 agggaaaacg aggacagcat ctcctttttc aaataataat acctttcttt catcaattgt
    93181 ctctgtagga ataaatgtag taacatcttt cccaggcagc tcagtttcaa aaaggccatt
    93241 tggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    93301 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tatattttcc
    93361 taacgtttgc gcaataaatt tgtaactttc tttattgcca aaacgggaac cgcaaaaaac
    93421 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    93481 tttttaaaat aaaagactag attttgtagt tttttagtgg tatatagatc aaacaccact
    93541 atatatagta tttgcaaaaa aataattttt cagctatttt acagtaacca aaaaaataaa
    93601 taactagtaa atataaaatt gaaacgactt acttatagaa gaaattggga tttcgtcctg
    93661 catgtcaata acacgcatta tttaaatagt cacacacttg tttctcctat taatgaaaaa
    93721 tccattttat tctcgtgtta tgtttcacca acgcctgatg tttctctaaa taaaaaagcc
    93781 gacgaaccaa ataatcgaaa tttgatcatt ttggttcatc agcttgttcc caaacctttg
    93841 tattaacgat aaatagctgg attcgtaaaa ctatcacttg gatcatcact gctggaagaa
    93901 aattcggtga taaccgcgcc tgttgcacct gcctggaacc agtgtttaat attttgttcg
    93961 atcgtgtact gatctccagc gtgaagtatg atttcttttt ttgccgtata gtaacgttct
    94021 tgcccctttg gaataataca ggaaagtttt tttgtttctt ttccttcgac ataaagatat
    94081 actaagcctt ttcgacaacg aaaggtttcc ttttttcctt cattctcatc tcgaggtggg
    94141 tgccgatgct ctggacaagt ttgataaggc aataatacca gttctttccc acagtatcgc
    94201 tcattatttt cataaacaaa taaattcaat ccttcagtct cgattgctcc taatccaaaa
    94261 tctgtatatt cgattctttc taattctgtt tctttcaata ctactcctga atctaggaac
    94321 atttgttgta ctcttttctt gtaatccatt ctgttcctcc taactgattg tcaagtcatc
    94381 ccaagatatc tcttcttcct ctttttcttc ttggatgact tctgcatttg ggtcaactgg
    94441 ttttttcaat aataaaatag ttattcccgt agcaaaagaa ccagctaaca atgcaccaat
    94501 gaatgcccat ggtctggtca tcgtagcgac tgcaaacatt cccgatgccg gaatacggct
    94561 atctgcgccc caaaacatgc tcaatccacc gccgactgcc ccaccgactc ccgttgctat
    94621 gacattgcgt gccaagtcgt tcaatacgat agggaaacat ccctctgtga tctgacagat
    94681 ccccatagga aaagcggtct tcaatgtatc gatttcttga cgattgaata tatttttacg
    94741 aatgattttc cctaagaaat aagcgatcgt caagccgaat ggggttacca tcgaagctaa
    94801 aatcaataca gtgattggtt catagattcc ttctgaaaat cgtgccacac ccccttatcc
    94861 agaataaatg gtaatccaga aacagccggg tatctgcggt aataagccta accaaacgaa
    94921 aaatatgaga agctaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca
    94981 gggcagcttt ttcacaacct ctttttttat ttatgtttca taatcaatat ggtaaggatg
    95041 cagctccgta tggtagaccg cctccgaata ttcaatcatt tccccaattt gcgtagacga
    95101 tttccgtatc cgtttgatgc cgatctgtgc gtctgttttc agaatttctt gttcttcttc
    95161 tgttaaaaat actgccgaga aatcatcaga aaatgagtca atagtatatc cttttttcgt
    95221 cagcaaacga tacaaagatt ctttgccaaa ttcttctctg ctgaattgca tcatttcttg
    95281 tggaagataa tgaacaaaat aaatataagg ctgattgttc aaataatata agcgcttaaa
    95341 acaaacagct tgctcaccga aaaaggaaaa caaaggatct tccttcttta tatggaccaa
    95401 ttgcaaatcc agcgtttctt tccggacttc atatcgggag ttctccaaga tttgcgtaaa
    95461 gcttgccgct ttggataatc gattgtaagg acgattgctt agaaccgtcg tcccacgacc
    95521 acttttcttt gcgacatatt catctgatgc cagcagttcg attgcctttc ggacagtgat
    95581 tttgctgact tgaaacagtt cttctaattc actttctgtc ggtaacatcg aaccgactgg
    95641 atattttcct gagaaaatat cttctttaat tttatctgcg acttccaagt atagtacacg
    95701 tgtccttttc ataacgtcac cctttgcttt actattacta aaagaatagc acataaaaaa
    95761 taaaaaaaga acacaaatca catgtgagaa gtgtgttctt tttcattcag ctgctttaat
    95821 tacaagttta cgccatttga ggcaattact tctttatacc aatcaaaaga ttttttcttt
    95881 gatcgattca gtgtcccatt tccttcatcg tctagatcca catagataaa gccgtaacgt
    95941 ttactcattt ctcctgttga tgcactgaca agatccatac atccccaagg cgtatagccc
    96001 atcaaatcta caccgtctgc tactgcatta gccatcgctt cgatatgacg acgtaaataa
    96061 tcgatccgat aatcatcaat aatcgtgcca tcctcttcca tatgatcaat ggctccaaga
    96121 ccgttttcaa caatgaataa tggtttctgg taacgattgt ataattcatt caatgcgatt
    96181 ctcagccctt ctggatcgat ctgccagccc cattcacttg cctcaagaaa cggatttttt
    96241 actcctccca gcaagttccc ttctgaagtt gctgcttgtg gatcagtctc attgattgcc
    96301 gtagacatat aataactaaa tccgatgtag tccactggat attcttttag caactggaag
    96361 tctttttccg tttgctctaa tttttccgga tcgactccat attttttatg ggtacgcgct
    96421 gtgtatgcag ggtattctcc acgtacttgg acatccgtgc agaaaaaatt gaattcttgg
    96481 ttttggatca ttgttgctgc ctgattgact ggattggcat cgatgctgta agttgttgca
    96541 tagataatca tgcagccgat ttggatatcc gaacgcaatt cgtggccgat ttttacggct
    96601 ttgctacttg caacgaattg attatgccat gcttggaaaa tattttggta ctctcctgct
    96661 ccattgcttt ttactaatcc ttgacttaaa gctggaaagt gaaaggccga attgatttca
    96721 ttgaaagtca tccaatattt gactttactg ctgtatctct ctaatactgt ctgagcaaat
    96781 cgttcataga agtcgatcag ttttcgattt ttccagccac cgtattcttt tgccaaatga
    96841 agaggcattt cgtaatgaga aatcgtgatc acaggttcaa tatcatattt taagcactca
    96901 tcaatcagct gatcataaaa ttctaatccc gcttcgttag gagtaccttc gtctccattt
    96961 ggaaaaattc gtgtccaggc aatcgaaaaa cgataacatt tgaatcccat ttccgcaaac
    97021 aaggcaagat cttctttgaa acgatcgtaa tgatcaattc ctcgatgatt cgggtaaata
    97081 tatttttctt gatcaatcgt ccagtcaaat tcttcactgc cgataatcgc aaagcgctgc
    97141 ttacctccag gcatagcatc tgcaacggat agcccttttc cgtctcgatc ataagcccct
    97201 tctaattgat tggcagcagt tgctccaccc cataaaaatt tttctgaaaa acgtgattgc
    97261 atcctgattt cctccttatt ttacaattgc cagaacatct tcgtttgcaa taatctcttt
    97321 ttggtctaat tcaagtatat cggcaaatgc atcagtattc gtaatgacaa ctggagtgat
    97381 tacgctatag ccagctgctc taattgcttc gatgtcaaaa cgaatcaata aatctcctgc
    97441 tttgacgaga tccccttgtt tgactaagat ctcaaagcca ttcccattta attctactgt
    97501 atccatcccg atatgaatca aaatctccgc tccgtcagtc gttgtgatcc ctacagcatg
    97561 tccagttgga aaaatagtcg tgatctctcc gtctgcaggt gcaactagtt ctcctacttc
    97621 tggatcaata gcgatacctt ttcccatcac gccagaagaa aaaacttggt ctggtacttc
    97681 actcaatggc agtactttac ccgttaaagg actttttaag gtatgccgtg ctgaagtaat
    97741 cggttctcct gcattcgcat gtttgttttc taattgattg tcttgtgttt gttcgtcaaa
    97801 tcctaaaatc aatgtaccta cgaatgcaag aacaaaagca attcctgtag ctattactgc
    97861 tactgtcaca tttgactcta caccatccac cgtattaatg aaagtcggaa tcgtcaataa
    97921 gctgaccaaa ctgctggaga aagcttttac tccggaaaat ccgataatag ctccaccaat
    97981 acctccagaa atacaagcgg caatgaatgg tttcttcaaa ggtaatgtca caccatacac
    98041 agttggttca gtgattccaa ataacgaagt cactgtagaa gaaatcccta atgctcgaag
    98101 tttcgtatct tttgttcgca atgctacagc taaagcagcc ccaccttgtg ccaagatggc
    98161 tggaagaagc attggcatca atacatcaaa cccatattgt tcgatattca aaaacatgat
    98221 cggtacgaat ccccaatgca tgccgaacat cacgaatact tgccataaac ttcccatgat
    98281 tagacctgct acgattggac taaatccata aatcgagttg aagagaccgc ctaaagcatt
    98341 accggcaacc gtcccgattg gtcctaaagc aagaaatgtt aatggaacca tcactactaa
    98401 aacgacaagc ggaactaaaa tcatttgcag aaattgcgga atcacttttt ttacgaatgg
    98461 ctctaattta ctttgaaccc atacggctag aatgattggt atgacagaag aagtataacc
    98521 agatggtgaa agaatcactg ggatcccaaa gaaactgata gcttctcccg caccagcaac
    98581 tgctgtgata gctggataca caagtgccat agcaattgct actgctaaaa actggttggc
    98641 tttgaacttt ttagctgctg taaaagcaag catgacaggt aagaaggtaa agacaccatc
    98701 tgctgcagca aacaatattt gataagtacc gctatcagct gtcagccagc ccatcaccgt
    98761 tgccaatgat aagaatcctt tcaatacccc ggcggctgcc atcgccccta gaaaaggtgt
    98821 aaatatacta gagataatat cgatcagacg attcaaaata tttttggact cttcttttcc
    98881 atcctccgaa actgcaagac cttcctcatc cacgatcgat tgataaacat ctgctacatt
    98941 actgccaatc actacttgat actgaccgcc actttgtacg acttggatca cgtccgggtc
    99001 ttcttttaac ttttgcgtat ctgctaaaga ttcatctttt aatctgaatc gcaaccgcgt
    99061 agcacaatgt actaaacttt tgacattctt ttgtcctcct accgcttccc atacacgacg
    99121 accaactgct tgattatcca ttgttttttc cttctttctt ttttatttaa aaaataaaaa
    99181 cccagattga aaaaaacagt cacaaacatt cgtgaaccta ttttttcaat ccaggttttg
    99241 cctgccatac agtaacaatc cttatgattg gatgcttttt ttctcactta ctactctttg
    99301 aatatgaatc gttaaataaa tctgctctgc gatccccatt tcctgttttt gcgtttgtcg
    99361 aagatactca ttgatccgtt cgacacaatg aaacgctttt ggatactttc cttgaactaa
    99421 tgcgtagaga aattcatctt gctcgtcaga tccttcccct ttgagataac gctgggcaaa
    99481 aaactgcaga tgagtaatga tacgctgata attcaaagat tcttcatcga acacgatgcc
    99541 gaagaattta ctgatgatac tcagtatatc tcttaccatt gtggtagcgg tcatcgtcac
    99601 ttgcatgtct tcgtttcctt gttggctatt cacaaaatga aaggcaataa aaccggcttc
    99661 atcctcagaa agttccacac ccgtcttttg tttcacgaac aaaagagcct ctttcgctgc
    99721 ttgatattct ttcggataaa atttttttgt ttcaaataat aatggattag gcagttggat
    99781 attttctttt gctcgtgtga tcgcataatg aagatgatcg gtcaatgtca gataaatatt
    99841 aggcgaaaat tcaactttta gtttatcttc agccagttcg actacttgtt tgacccattc
    99901 gatctcctct gccggaatat cagccatcag ctgctggaat tggtttttag caaaagaatc
    99961 tcttaaaaca aattcttttt ggaccgcttc tggcgaaatc tcgtccccaa tctttttttg
   100021 aaaagctagt ccttttccca tacaaacgac ttcttccccg aatccatttt tcgaaatgac
   100081 gacattgtta ttcaaaattt tatcaatttt cattttgctt cgccctctca agaaaaataa
   100141 aaaaacctag aattcgccta ctgtttctta gctgaattct ggttttgcct gccgtacagt
   100201 aacaatccat ttcttattca aaatcatcat accatcgaat ttttatttat gcaaaccttt
   100261 tcatataaaa aataaaaaaa ctggaacaca agtctatcta gaaaacaacc aaatcagaga
   100321 tgcacctctt tgcgtttctc tttttttcag caaattggtt tttccttatc gattcatatc
   100381 ctttggctgt ttttaatctg taacgactta tgtcccagta tcctaaaatg tttttgtctc
   100441 tagattgaaa gcagatctca tatcatctgt cgcatctttt atcgatgtaa cactttccag
   100501 ttctccttgt acaactagtc gcgtgacggc gtccatgtcg ccacaagtaa ttagtgtgac
   100561 aatttctttt ccctcgactt cattcagtaa ttgggtatct gtaggagcaa ctttttcttt
   100621 aaaaaatgtt ttgtatgtat acacattttc taaatctgtc aaatagatct tggcacccat
   100681 ctctaaattt tctagcggtg taaacagcaa ttcagggttc gttgcacgat ggcttgctaa
   100741 ggcataattt ccttttccca tttcttgatc tggagataat gttcctgctc cgtaaagtag
   100801 tgacgtatta tccaagcctt tgaaaaccgg taaattgatt gaaactgaag ggactgcgat
   100861 actagcaatc actggtagat ctgttcctct catttgcgac ctcatgaccc cttctgtcgt
   100921 cattggttct accgcatcaa agtcaaaact gacatcttta tcgttattct ttttcagatc
   100981 ttcttttgtt acattcgcta tagcatattt atctcctgtc tctctgacaa aataatcctt
   101041 gatctgttca ttgaaaatca aagctagtcc tactaataac aagaggaaca agaggatatt
   101101 gactagccat ctttttgctt ttcctttttt tttcattttc tctccctcca gactcatttt
   101161 actgtttttc ttttgctctt tccaacagcc gaatgacaca tttctacaac tctgtgtatt
   101221 tttttcgtac acctgcgctt ttttctacag gatatttcaa attatttttg atcaattttt
   101281 cttcaatgat ctcatctaga tccaggtgaa ggttgtctgc catcatgtat gaatagatca
   101341 atacatctgc cagttcttct ttgattcgtt ctaattgtgt ttcttttact tcttccggcg
   101401 ttttccattg aaaaagttct aacaattcag atgcttccaa agaaatagag atagccaaat
   101461 ctttttcgtt atgaaattgt cgccaattac gttcatctct aaactggttg actttttcca
   101521 tactattcat ttgcttgcct tcttccaatc acttcttact tgattaatca taccattttt
   101581 ttacataaaa aatagatgat gcttttaaaa aaactcctgt tttcttttat ttaaaataaa
   101641 tgtcatagaa aatcaataat ttaggtagcc accatgtgta tgcagtagcg tgtcttcttc
   101701 ccttgaggct ttttgttttt cgatacgttc ttcttcccaa acagacaacg ctactagaat
   101761 ctctcttaat gattttcctt cttcggtgag cgagtactct actttaggcg ggacttggtt
   101821 ataggctttt cgatggatac tttcatcttg ttctaactct cttaactgct gggtcagcat
   101881 tttttttgtg atcgtaggga tttgcttgct tctccacgcc taaaataagc gatattgctc
   101941 ttccctttat ctggtgtaag caaagcagtt agcattttga cgatcgtggg ttccgctttt
   102001 cacttcttat ctacttagta tcatgaagta tactttcgta ctaatactaa gtagcggaag
   102061 tgtcaactac tttgtttaac tacatagcaa aagaaacaga agggtgacaa cggtcatcac
   102121 gcttctgttt cttttgaata tgctgtgttg ttgtctctat ctccgtgtag actttcctca
   102181 aacttatcct ttgacagtaa gtcagtaggc tgatttgctc tttctagatg ccagatacat
   102241 ttctacaaaa aaatagtgat tctgttttga aaattcgatt tttagttctg cattttcgta
   102301 aacatccaac atttttttcg cactgtatag acctaacccg ccattttttt tagaggtttt
   102361 tcctcttttg accatctccg atagcgtcaa attcgttgga tttgccttgt tcgctatact
   102421 aaaacgtgat ccctcttttt cttccagcag attgatcgta atcggactct gatcggctac
   102481 acgatgatcg aatgcgttag aaataaaaat agataaacag cgaataaaaa caaccagatc
   102541 aatcgggatt gttttcactt cgacaggaat aactacttgg atcgggattt tctccgattc
   102601 tgctttttct agaaatttta aaaagacacc cctgacagct ggggatacaa tatttgttag
   102661 ctgttgtatt tgcggttttt caaaaatagc tgcagaatga tcttctaata tttctaaaaa
   102721 ttccaacgtt tcttttttat tttcctgaag agtaattttt aatgaaaaca gtagactttt
   102781 ataatcatgt ttgaattcct ctagatgatg gtaatattct tcatttattt gatacagttg
   102841 aagcgattcc tgatgttgtc tttcttttac ttggaaactg atcaacataa tggaaaaaaa
   102901 caaagaaaaa aaactgatga ctatcgacaa aagagataga agcagataat tagatgagcg
   102961 gaagtgaaca cttcccaata ccaagtaaat cacgccccag cttaataaaa tacaaatacc
   103021 tccaaaatag tgttcagttg gataagtaaa aagcaagttg agaaatctcg tctgtttgtc
   103081 tatacttttt actaatagat agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt
   103141 tccaagatgt ctctttccta aataatccat cagatcaaaa accagaaaaa agctgagtcc
   103201 agtgatagca aaaaaaagat tactgatcaa acagctgata aacaggtttt tcgtagtaaa
   103261 ataaaaaaga aacatcaata ataagccaat taggtaagga aagctcactt tagggatagg
   103321 aagtaaaagt gtcaccacca tcaaagctat acaaccaaag ctgtttttta ctagcagttg
   103381 tttcgaaaag aataaataga tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt
   103441 cactgttgcg accatcttat tcctccattg ttttgtttca aaatgtacac atattttttt
   103501 aataggagcc tgcatatgaa aaagtatttt atcatagaag ataaccagtt tcacagcgaa
   103561 tggatcatca gagaattggt caatcatcat atcgagaata tcgtgcagct cacttctatc
   103621 tctcaaatca atcagttgat ccagttgggc actattgata atcacgatgt tttctttatc
   103681 gatatcgact tgaagcaatc actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa
   103741 aatgcacata gtaatattgt ttttttcacc gcttatacga attttgcagt agagatcatc
   103801 aatcggaaaa tccttccgat tggatatatc agaaaagaca ggaatttatc tgaacaaatt
   103861 cgtcaagtgc tagaacagat caattggaaa gaacagcaga tcgtaaatgt cgataaaatc
   103921 tttattgatc aaaaaggtgg aagtctcgtc ctgtatcctc gggaaatttt atatattgaa
   103981 agtatcaaag gtttgaaaaa ccaagttatg gtcaaaacaa ctacagaaga taaattagtg
   104041 aatacttctt taaaacaatt gaagacagag ttagaaaagt atgcttactt tttactttta
   104101 aaatcttact gtatcaactt atcgcaattg cagaaaattg attctgctca ttatgtgtta
   104161 gaatttttca acggtgactc cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa
   104221 cgtttccatg acttccaaaa aactgcttcg agttgatgaa taagagctga gacgtaagca
   104281 tagcagattt cagggagtgg gccttgaatc ggctccactc cctgaagtcg ttgatatgat
   104341 tttacgcttc ctcttctgta ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt
   104401 tgtctacagc ataattggat acaggaacaa ataattccac tgattgtctc ccttgttcca
   104461 cattctctcc taatgaataa atccatgtgc caatcccttc atttgctcct gcagttacta
   104521 gatctactgc ctgatctgct gggatttcga ttccttcagc gccagttcca gaaacggtag
   104581 atggcattcc actgctatta gttgcgctat ttcgaatcac ggcgtttttg aatttcacct
   104641 ttgaaccagt tagtttattc gtatcagatg ttgtaccaat aaattctgtt cgtttgactg
   104701 ataatttcca accggcataa tttccgcttt tatccgttac ttgtacgaaa ttctgtactt
   104761 gtgtttgtgc tccagtactg tccgttccgc tttgtaatgc agcatatact gtttttgctg
   104821 tacctaactt gattgttcca aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg
   104881 atgttccctg atttggttcg tcaggtgtaa aaggagtatc aggatcattt ggatcaacag
   104941 gaggaactgg aacaacgtct tcaggatttg cagcatctgg tttggtcaca gaaatcgttc
   105001 cttctgtagt ggctgagctc ttattctcag caaaaacact ttgtgtacat aataatagac
   105061 ttccagctag aacggtactt aatgcaattg tttttttcat ttctttttcc ttctttcttt
   105121 taaaataaat aatccagcaa ataagccaat aagaatgact ccaacaagta tcagccatcg
   105181 ccagtcaaat gtttctggct gattttttaa atcaatatcc ttttcattca attggtccgc
   105241 tgcatcatca gttatttcaa aatcggtatg gagattccag cgtttttctt tccaagcacc
   105301 cttcacaacc gcttgataat gtcccggagc taattttttt ccttctaatg ggacaagtaa
   105361 atcaaaattt gtattgggtg ctacatctat tttttctttt tttattgaaa ggtaaggctc
   105421 ttttgtttct tttttgtaga tttcaaccga tgtagtcaag ccgaacatat aagaagcatt
   105481 tggattttga agattcgcct taatcacatt tcttccatcg ctttgatccg cataagcttt
   105541 ttttaatttc aaatctgggg aagactcttt ttcgctattt caaatcaaga ttgctttcac
   105601 ttgagaaaac tgattgacga caccaactgt acttttgtta gaaacgttct cttttttttc
   105661 ttgaaacgtg atccctcctg cgaccacacc agccagctct ttttctggcg ctttcagtga
   105721 aagtgtctca gtgaccgcac tttgaggagg aacagttatt tctcccactg tttttgctaa
   105781 gtttttcata tcataactta atgtttcatc aaatttactt tttgattctc catattcgat
   105841 cacaccgttt gaattcgtcg tagcggagtg gatcaatgtc tcaactgtta ctgatttatc
   105901 tgttcggtta aagaccgtgt aggggatttc ctgtgttttc ccaggctcca tccttacatt
   105961 tatatatgtt ttgttcttat caatctgatt agccggtaat tcaaaatcta ccgcaaaatt
   106021 gaattcggag gcatggacag gtgtctgcca aaggatgaat ccaaggagaa ggatcataag
   106081 taaagagcag ttgtttcgat gacttttatt ccatattcga ttcatcaatc atcctccttg
   106141 atctttattc tgtcggcgca tctgctaatg tccatgttat ctctgctgag taattaccag
   106201 ctatctgatt ttctttggtt aagacaagtt taaacgcccg accgttattt aaatattctg
   106261 aataatctgc ttgcacattc gttgaatttt gtcctctgac aatttccagt tcttcttcat
   106321 tcactaattg atactcctct cctttcttga aatataggac atcctgcata gaaaatcctg
   106381 catcatttgt gaaaggctga gataatttta tcgttaagat ccacggattt ttcgtttctc
   106441 gctgatcata aatccataat ttttcctgta tatccatttg gccaaatgct tcttgtgtca
   106501 aaccagttgc tttgatctga ccaaaatcga gtttggaagg ggcaagaaat gagagaccac
   106561 cttctactac gatcttcgta gcaggtgcaa aaggcggggc atcatgataa acaactggcg
   106621 tatcacttgg attgtgattc ccaatttcat cgttggtact tggcttgttc tgcacaccct
   106681 tagcagtagc ttctcctgct tggtcgttta atacaatttc taatacatct cctgcaaaaa
   106741 gttgattttc tgggatagaa agaacaaatt cccctgtatc atcaatagtt gtttctgaca
   106801 aaacagcttg attttttttc aatgaaacca aggctcctgc ttcacctgta cctgtgatcg
   106861 tgcgagaagc tgcattgatc aatgtggtac ttaaattagc aggttcagga ggaatcacat
   106921 cgactgtcgt tttggcagag tccagttctg tcacttcttg gccacctctt acttttttag
   106981 cagcaacaac ttgcacttga tcctccgttt ttaatctctc atcattgggt acttgaactt
   107041 cgaacaagcc gccttgttcg ccttctcccc atgggttata tcctgcccct tcattttcat
   107101 ctgtttgtcc ttgcgtgaga gcagtaattt ctacagatga tttcgtcgta tctgcgtaag
   107161 taatacgtaa agtcacttct acttcgcctg tccaagcatc tcttggcttt gcctcgacac
   107221 cttcgggaac aacgacatgc ccatagattt ttttatctgc attcgttggc actcttaagg
   107281 attcaatcat cgggagctga ttattggcac tgattcgatt ataatttttc attccggcac
   107341 ctgtctgagc aaattcattt gttaaacctg cactagggct tgatacgaaa ttgcctaagt
   107401 cggtcccatt gatcgataag tctgtcatat cagaaaaata atccgggtct ttttcaaagt
   107461 tatcgaatgt tgcgcctgtt tttcgccata aagcgatttc tgaatgggcg atctttaaag
   107521 aagaatctcc atttccttgg aatacccagc cgcctatgcc attggtctcg cttccaatcc
   107581 gtttgttttg gaaatcaaaa taggtaggag attctaagtc cacattcaac acatccccac
   107641 tgatgatgcc ggtcttatct tcccctgttt tcccgaccat gactaaataa gttttttcac
   107701 ctgccgtaaa atttaaatca atatctccag ttgtatctac agttgctccg gaatctgcgc
   107761 gtatatcgat atttgatttt tctccagtga ctgtaaaatc agctgaacct ggactttcta
   107821 gaaaaccaga agcgtcataa aattggatcg cttgatttcc ttgattgagt ctgttcacgt
   107881 aagatttgcc atcacctgta tttcttaccc tcaatgagcc tccattggtt actgtcagct
   107941 tttgcgtacc attctcaaaa cgaactgcgc tggaacgccc actctgctta tcgatgtcga
   108001 taactgatcc ggctccttct acgatcaaag aaaggccttt tcctacaaaa cgcatcccat
   108061 tgttcccggg ttgatcgtca tctccagcca ttttgaccga gatttgtcca ccacttgata
   108121 catggatttg actagaatct ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg
   108181 tgattttccc accattcgat acagtgactt tcgatcctga acctctcatt tggatgattc
   108241 cgcgattgtt gtcattggta atattgatct ttgtatttac cgaaaattcg cttcccgaac
   108301 cactgacttc aaacatgaca ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa
   108361 cgccatctgc ccgatcataa tcaagagaga cctttgaacc gcttaagata tggattcctg
   108421 gcgctgtttt tccactacca gtctcatctc ctgaatctag aaaaccgtag aatttcccta
   108481 gatacggtcc ataaagttga ctaccattga tcacattcaa ttgatagctt ctacaaaaat
   108541 aggctttacc cggagcggca ttcagtcctt ccttcactgg ttggacatcc aacacaccgg
   108601 aaacaccatc taatgtgaca gttgcacgag gagcgtaaat cagtcctaat ttggacccat
   108661 catacaaatc aagtgtcccg gtaaatttta tttctcccgt aaaatgatat ttgcttcccc
   108721 aaaaatcggt catataagca atgataatgg catttccatc gatatttggt gtcaatccac
   108781 ctggatgtgc agctgttcct ttaaaattct ggaactcgat cgactgaggt atctcattga
   108841 tataaatatg gtgtgtgccg aaattgtgag cgaatccacc aaaatcaata acgatatctc
   108901 gtttgatctc taattccctg tctaaagtca aaggctgaat cagtcggatc ttagttactg
   108961 attcgtctgc gagaagctgc gccagttctt ctgttgtggc agcttcgctg tacccatccg
   109021 ctgaaacagc tcttgccagt gccgcttcac tgctatcaga cgtttgttca gctacttctg
   109081 aggtctcact tgtcttattt tcttcttgcc ttttattagt agaagcctca ctttgagatt
   109141 ctgtagtttc agaagtaatc gattcttctg aaaaagtagt cttgtcaaaa ctttgattct
   109201 ctgattgcgt tgattcgttt gttttctcta catcggcaaa aacggatgtc accatcaaga
   109261 gagggaagac aaacaaaatc gacgcaatca gcttttttac acattgattc attctccacc
   109321 atcctctctg acgttttgac aattttattt tatcgtttta atctatttcc aattgtactt
   109381 tcatatttaa aatatgtatt tttatggaaa aatttttcta ttaggtgtaa atttcttttt
   109441 tgtacaaaaa aaaggctgtg acaaaagtct tgtcacagtc ccttatcccg aacaagcggt
   109501 gttctcccgc caactcctcg gtcacaacct ccattttaac aataaacctg taaatgtgag
   109561 ggcaatactt tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc
   109621 aattcttcct catcagctaa tgacacaact aattgctcac aagtaaggta ggctagattt
   109681 ttcgtagatt catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa
   109741 gaagaatctt ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt
   109801 aatgtttcaa aaccgccgac tgagttagtt agtcctatca gcaaggtact gctctcaatt
   109861 tcttccttct ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt
   109921 ttgattcctg acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca
   109981 tcattgattg attcagggat agagccaatt gcgacgacat tcataaaata gtcgtcattt
   110041 atttttccaa tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca
   110101 ggttctagcg gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat
   110161 ccgaaatttg gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca
   110221 tctccgccca tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt
   110281 tttgcatccc ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat
   110341 tttgctatcg ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc
   110401 actaataaaa cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga
   110461 tagcataaag aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt
   110521 tttgacaaga taaaacatcc atactatgaa actgaggaaa attaaaacaa tgatatagga
   110581 aataaaagaa aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata
   110641 aaaacacaaa aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct
   110701 tatagcgatc agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat
   110761 gtcacggcct tgaataataa atgcgacaat ccccgctata attagtgtgc ctaacaccaa
   110821 aacggaaaat tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt
   110881 atttgtgaga aaaacaaatc cgtatacgtg aataacttct agaaataaaa gaaatttgca
   110941 aaaccactcc atttatctac ttctacgtgc attttttgat attgttttct ttatttaaga
   111001 ttatacagaa aaatatctag ggtcgctgga ttctaagtaa aaaaaatttt taaaataaaa
   111061 agaactaaat caagtagtaa ttaaactact gttgatttag ttctaataaa aagatttaga
   111121 tgaacagttt tattgattta aatagttcat attttttatt tataccgctt atttactact
   111181 tgctgtacta gaatctgaag tgaatgtact caaaacattt tcaaagtctt tgtctttgat
   111241 ttttacatta gcgtctttca atacttcgcc aattacttta ttctggaagg tactgtcact
   111301 taattttgtg tctgttgcaa tttctttcaa ttcttttttg tatttatcca tgtcgttgcc
   111361 tttgttttgg tttttcacca ttttaaccac ataatattcc gtagtataag ttgaagcatt
   111421 ggtagaagtg atcacatcag aaacttgacc atctttcaac ttaaaggctg cttctttgac
   111481 ttctgccgga accgttgtag tagtggaatc aaatttcact tttccgccat cttcttttgt
   111541 cgtagtatca gttgacttgt ctttggctaa tttagagaaa tcgtctccct tatcagcagc
   111601 tttcttcaca tcttttgcat catcttcact tgctacttgg ataatttgag cttccacttc
   111661 tggatggaaa gattcccaag ccgtttttaa atcttcatcg gtcaatttga tatgtttctt
   111721 tagaccttct tgaaaggcta gactttgttt gatactgtct ttgaatgttt gttctgtgta
   111781 acctgcagat ttcaattgtg aatcaaatga atcacccaat tgtttctttg tttgatcata
   111841 ttgagaatcg atttctttgg ttgatacgtc atctccatat ttatcttcaa atacttgata
   111901 aacgatcaac tgttgcacag cggaatctgc tccttgcatt tttttcaagc tatccattac
   111961 ttcttgttcg ctgattgtac cgcctttata actaacggct gttttttcac cggcattcgt
   112021 acagcctgac aataagccag tgcctagtac gatggcaagt gctaaaaatc tttttttcat
   112081 ttatcaattc tcctactttt cgttttttgc gaattgctaa aaacaaaggt gttcaagatg
   112141 aaaatcatca agaacacctt tactaatctg ctcgatacgt cctgaataga tataaggcta
   112201 cacaatccag aaagttcgct cagcagaata aatgtcaagt agcacatcac accttaatat
   112261 actacttctt ttgttaccgt ttttcaagtc tatcttgcgt tccgtattta acgtaactaa
   112321 ttcttagaac aaatgttatc gattttgttt ttcttcttcc gctgctggga tattcaaatc
   112381 agacactttt atgttcgatt ttccagacat ttttttgttg tttttattat gatatatttt
   112441 tatacaacta aaaatgaata tattaaaact accaaaaata acttttaacg ctgtttcatt
   112501 atatgttttt tctattttat ataaactttt ggatacttaa taccaaccgg ttggattgta
   112561 ccaacatgag aatgtgtcag ttctatttcc tgtcgcactt gcataggccc atactccttt
   112621 tgttgcattt gctgatcgaa tagagtttcc aactttcgct tttgcaccgt gacgattata
   112681 gacacgacta tagtggttag aatgacctgt ttttctcgta caagttgtat cactataata
   112741 aacccatctt ccagagctat gattaattgc tgttctagaa atttcccggt ctactggtaa
   112801 atttgactta ataatattac ctttagcatc aaaatgaatc tctcctggct ttaaagattt
   112861 gtaatagatt tcaccgtgaa ctgaatttgc ttccacttca gatgacatag cgtttcctcc
   112921 aacaaaacct atccctagtg cacaaataat aactgctttt ttcataaaac gacatccttt
   112981 cctttttgat ttttaaagca tgatcttctt gtttcctatt ttggtcagat atggatcatg
   113041 cgtgactacg ataactaacg taccttggga agcttgttgc gataagattt caactacttt
   113101 atccttattt cttttatcca atgacccagt cggttcatcg gctaaaatga ttttgctatt
   113161 cttcaataag actctcgcac aggcgattct ttgcttctca ccaccagata atgtgtagat
   113221 aggcgtactt agcttcttgt ttatatttac ttttttcaat gcctcatact ttgctgttcg
   113281 cttatctgaa acatttaaat tcgaaaactt caatccaata tctaaattaa aatcgattgt
   113341 ttcatattca atcaacccaa aatcttgaaa aagaaaagat atggtatttc tttttatcaa
   113401 gcgcttctct ctcccagtta attgtttcgt ctcttgatcg aacaaataga attgtcctga
   113461 actgaactga tcattcaatc ctaatatatt caataacgta cttttacctg ctccactttc
   113521 tccaacaaca gtcacaagat ccccagcatc cgcagtgaaa cgatccaaat gaagaatcgt
   113581 tttgtttcga atgatttttt tcgcaccttt tatcactatc aacggctcca attgttaacc
   113641 tccattcatc agcaataact gacttttctt ttctgtagac aaaataaaaa caactgaaag
   113701 aataaaatca atgaagctaa agaaaaaaat attaaaaaga tatttaggct ctctttctat
   113761 aagaaaaagg aagattacac ttgtcgttcc ccatttgatg aggttcatca tgaagaaatc
   113821 tttatacgtt tcaaagagcg aataaccact tgaacgaaag atagcgattt tccgacaatt
   113881 gtagacaaaa tatatgcata acatgacata gctcaacaag aagaaagaaa agaaagtgag
   113941 taaaaatatc tgtatcaagt aatgaaatga tctgcttaac actttgacag ctatctcttc
   114001 agtctgagta tacggaaatg ataactgcaa gttatcacgt aactgacttt cttcaatcag
   114061 ctcaaataac ctttctttcg acaaagagcc tatcttgatc tttaacggtg gatagatttc
   114121 tccgctgaga atatttcttt ccaaatacat actgtttttc aatgtcagta tttcaacaac
   114181 cggataatgt tcgatccagg ggatagaagg aataaatgta taaatgggtt gattgttttt
   114241 tataaataga aatttcggtt ctttttcact gatttcttga taatattctg ttatttgcgc
   114301 caggtcattt tttaaatctg tatgtctata tcgttcaggg atcaatataa cagggtgcgt
   114361 ttctttttcg gttatttgga tcaaccttcc agagacatca atcaaaggaa atttcttaag
   114421 ataattcaga tttattttta tccctctgcc aaactgttca ttttcttcat tcaagtaatc
   114481 tgatacattg actaatatac ttccgttttt attcaaagtt tcatataatt cctgatctgc
   114541 tgctgatgca aaacgattgg aataaataaa atccacgtgg tttttcccga tggttactgg
   114601 ataaaataca tgatagtcct caccagcgat ttcttttggt ggttcatggg aaacagctaa
   114661 ctgtgaagaa gaaaaaacga tagttgctaa ttcgacattg gcaacggtca aaagaagaga
   114721 aaacaacaat ccaaatgtaa aaagaatgta ttggatatag ttgaacgctt ctcttaacga
   114781 agcgattttt tcaactaata aaagagaaat caaagagata ccatatattc cgctgaacaa
   114841 gaaaacgtac atctggaaca aataaagaaa ttgctcctta taatacataa aaataaaacc
   114901 accaatgatt cctgcaatta tgaatgtaat aatgattttc tttttcccca ataaacacca
   114961 caatacttga tgtaatgaaa agtcattatt caacaataaa ctgatttctt tattcatcaa
   115021 taagaaaaag gatgataaca aaaggaataa aagaatcaat ccaatagtta aatacactcg
   115081 ataattatat acgtctgtat ttgaaataac cgaatacgaa tagctatttt caaagtcttc
   115141 aaaaatatta gtggcataat cagtgtgaaa tgcttgattg aatcgatcag tgaatagtga
   115201 aatgacccgt tcataggctt ctttttctct cgtcatcaag aaaaattcac cttcaaaatc
   115261 ttttattttg atcgattctt caatggacgt tacgcgaaag gaggtattag aatagggtac
   115321 cttctcgtga gaagatttga catccccttg ataatacaat gtcatttcaa gctttggctg
   115381 tatcttgaag ttccaagatg tatctacttc attggattca tttactgttt ttttcatata
   115441 agaaagctca gtttctgatg agacattttc aaataccatg attagctgct catattcttc
   115501 ttgcccaata aaatgttgtg gcaatcgaat cggagtataa gtatctgttt gatggaaaaa
   115561 ttcatttgtc cgatagagta cctgtttatt tatactaacc aaggcaaaac cactcatgat
   115621 accggtcact atgattgtga gtaaaactgt gaactttttt ttgattatca ccactctccc
   115681 ttacttaaat gattctttga taaaatcata ccgtttttca tcaaaaaaac gtatctatca
   115741 agtgtatagg tcatgtagaa taaatctata tactgtagaa tcgaaaggag aagaagatga
   115801 acaccggaga aaaaatacga acttaccgca gaaattgtaa gcttacgcaa aaagaattag
   115861 gcgaaaaaat tggtgtttcc gataaaacga tttctagttg ggaaaattct cgtacaatgc
   115921 cagactttga aatgctgtca cttttacatc atactttagg tctgccgatt gattttcctt
   115981 ctattgaatc agctaccgaa tcaatagaac caacattgcc agatgaacct cttgttaaca
   116041 agaaatctta ttcatcaaaa caacccttct cactcaaaaa gacactatct ttctttctgc
   116101 tcgtcttttg tttggtctat ctttttctta tcaattatca atacaaaaat gaaacgatcg
   116161 atcgattcaa tccttttttg aaaccacaaa tcggctatac cacactccct gtaggatacg
   116221 tggacaattc catcgaacgt tgggtatgta atgatatctt tggccatggt acatggctgt
   116281 cttttcacgc tggagaggta gaaaaagaga aaaaccatgc tgtaatcaaa cacaaaggaa
   116341 aacacgtcat gaacgttcgc ctgctcactt gggaagccat tccctcagat taccaagaaa
   116401 tcctcagcca agactatgac gacgccttaa aaagaagccc tcaggagcca gataggcatt
   116461 aagtaggaga tgaacgtaga gatagaaaaa atcaatgcac atgatcttat ccaaaaaaca
   116521 ggggtacaaa ttaattgaaa aagccttgag atgaaagttc ctaaataaaa aagttcagaa
   116581 tgataggtat ttctggctta tagtataata tttacgccac agcctcccaa tgattctttt
   116641 tggaatcata atcaaggatg gacattcaca aaaatatttt gctcattcga tccgaataga
   116701 ttaacgacca atctagtaag taatatagat tggtacgtag tcaagctgca cataatttag
   116761 atcaatttta gttctttttt atatttctat gatctgctca tttactttta tatgatggct
   116821 tacagtaaca taaataaata tgataatcgc tggtagttta tccaagagta ctcaataaaa
   116881 tacaaaatgg attgttcaag cgcctatcat aatgataaaa tgaaacaaat aaacgataca
   116941 aggaggaaga gactttgagt aatggcagaa caattttttc attgtaaaaa ttgttcagtt
   117001 gttaatctat gggatttcgg tagcatgggc atttttttat agaggaatca caccaacctg
   117061 gccgttttat ctcttgttag tgggaatatt agtaggaaat tttgcccctc ttcactatcg
   117121 tacgaacttt tttccaactg attctggcct ttttcttaaa acaaagacgc gaaaattaga
   117181 aaacattatt gaaatgacaa ttacctgtat aatcattttc ttagttatat tcctaaacgt
   117241 cttctaataa gaatctctta aaattagctt gttacagtta gttacctagt ttatataaca
   117301 cagttatctt atcttcctta tcaacatctt ttgccaactc tttgtcttaa attcgaaaca
   117361 tataagcaga gctacgaaca ccaagactgt ttcggctctg ttttttgcat caaattcact
   117421 cttatatagc tgataatctc ggctctttaa aagaaattat tttcgcttca ttttattata
   117481 aaaaggctag gaaccttgat gttatcaggt tcctagcctt tatttaagta ttaccccaca
   117541 gacccttcca tatagatttt tgaattacac actagtttaa actaaaacaa acaaggaaaa
   117601 acttaccatt taatcataaa attagctcat atgaatatat tagaatgtaa aatagactgg
   117661 tcaaaatctg gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttgata
   117721 ttctaaatca ttttttattg gattattccc ttgttcgatg tagactcaaa tcaatctctt
   117781 tacttgctaa catcctttat tttaccttga cctgttgata atagacagtt aaatcttgat
   117841 ttaaagtggg gcaattaatc aagaaaacag ggttctaagc ctcatttgtt agcttgttct
   117901 cttagtaata ttcctcgtga agaatcggga cgattgtttg gtcaataaac aatatctcac
   117961 ttgcatcgaa agaatatttc ttgaacaact gcatatcaac ttcttcaata cctacattcc
   118021 agttaatgtc tgaacctgag gtaaaatctt gtactggtat gaacatgaac ttatctttgg
   118081 ataggtgaat agatgtcact gcttgaagta ataaaaatct gtaaaaacgt gttcgtaaat
   118141 acttcagata attaattgct tcggtttcgg tatcagcaga aaatagcact aggtatgaat
   118201 ctgtaactac ttcattaggt ttaataactc tgttagtaga aatcacctta aacataccat
   118261 ttttgttcgg ttcacctgca tgttcgctag tcagtgagct actaatgact ttatactttc
   118321 caaccatatc ctgtccttta gtgatatctt ccagtggaac aaagcctacc ccaccactag
   118381 agattacttt tattgaattt ctggaatcct gcacttcttc ccctcgaatt ttcgttggaa
   118441 gcccaaatgg gttacgtgat ataacaaagt caccaacact agcgtgttcc ccaattttgt
   118501 gaattgcaga aatggcttca ttatatcgca caaacactgg aaactcgttc atttttcgct
   118561 cttgtgtatt tgatacacca tcatgttgat tagtatactg tgtcaatcca ttatatttct
   118621 tatcccacaa gaaatagcta acaccgccac caatactggt gtttgggaat atttctttcc
   118681 catttactat atccaccatc accttcaagc tagagctgtt aagcataaag tcacggtact
   118741 tatctaaccc tttaccacct gcaaaccact tcgaaggagt aatcatcgaa acatagcgtt
   118801 ctgataactt aacagaggtt tctacaaata gattatatag cggtgtagca ctagctcctg
   118861 aacctccatc tttctcctga taaggtgggt taccaattat cacatcaaat ttcacgtttt
   118921 tataaactcc tttcaaattc gttaaaccgt cattgattga acgtttcata ctcgtaacta
   118981 attcacttga gtattcaaca tttaccttcc atgcgttata accaatcaaa gtcctcttcg
   119041 ttatcgtctt agccattgga gttttagaaa tgacaaaaat ttgctctgat aatacttttt
   119101 cccatattgc ctctgaatct acccttccag cattaatatc gctttgttta aggagctgaa
   119161 catagtacat actcgttgct gcatataaag gatatagccc tgtttttgag tttatctcaa
   119221 gaatctttct attttcctta aaaatatcat ctgtacagtc agttttcctc catcgcacga
   119281 ttggttgacc gttctccgta gaattttggt attcttcatc aaagaaggaa agtccaccga
   119341 tagtgttacc aaggtgcata ttaacaactc gccatggagt caatacggtt tccttgtctg
   119401 gatttttaaa agtgctaaat aattcaccta ttttcctagt acgttcaata acatctaact
   119461 catcataaga ctttgcacgt ctacggatta tgcgtcctgc ctccacaaaa acgtctgcat
   119521 catagtactc aataaactct ctgaatcgcc ctttacttaa cccctctggc atgaactcat
   119581 tccatgaagc ctcatcaacc aaattaagaa acatctcaat agtaacgtca tctttaatat
   119641 caatgtccat cccataaatc atcataggta tgcgaataga aacgcccctc aaaatggaaa
   119701 tcattttatt tcgctgctct cgaagcctct ttgccctatc tattgcttcc tgttcctctt
   119761 tggtacgttg acgttttggt ttcttttctg ccctcgaagc tttttcatac tcctcttcac
   119821 tcataccgtt atctgaaaca atgaagtctt tcactttggt tggtttactt gagccaacaa
   119881 tttttttaag gtcttcaaat ttggtaagat cagcttggtc taaatttaat aggttgtcat
   119941 tgtaaagaga gctgtcttca aagccagtcc gtacagcttt ttctgcataa gccttcttca
   120001 gtgccgtaag cattcggtca acgctaaatt ctttcatcac attcccctgt ttaccaagga
   120061 taggtaagaa atttagcatg cgctctagtt ttttcttttg ctctgaacta ttaacctttc
   120121 ccttcttgct agttagtccg acacttccac tcataatttt taaagcacgg tcaggtgcaa
   120181 aatcaaagat gtagcagttt ttcttaattc caagctctgc atggttaaaa ggtgtctgtg
   120241 cacggaacgc tgcctgcaga taagatgtag gactttctgt gtttgaaagg aaaaataccc
   120301 ctgtccactc tggaacatta actcctgttg taagcttgcg aactgttaag gttatcgtct
   120361 tagacttagc tggattacca atagctttac gcaccaattc taaatctgaa tcactagcat
   120421 gctcactgtc acctgtcttg actacgtttg caatattgta atctttgaat atagggtgcg
   120481 tttcgagcac tctttcaaaa gcatttgcct ctttaacccc cggcatcagc cataaagtat
   120541 gccgtaagtt ttctctaaac tctggttttg agaaaggaaa attagtcttt tcatcgtttg
   120601 tagtaatata atcaagaaac ttgcgtacgt caccttcatg aagcaacttt gtttcatcat
   120661 cttccttaac cctaaagaat tctcggaagt taaatgcctt attttcatcg aaataatcaa
   120721 atttatttgg tatttcaaag gtgaacatag atactttcgg taaaccttcg tatgggttag
   120781 gttcattggg atgctctact gaccatttct ccttagcttc ttgctccata gtataatccc
   120841 aagtgaaaat attatcctca tcatacttat cgaaaaggtt aaatggtgta cccgaaagct
   120901 ctaagacctt ggtatgctct ttccgcaact tagcctttac gttattagct aattccgttt
   120961 cgtttccttc gtgagcttca tcaataataa taaaatccca gtcaatcgct aagaattcat
   121021 agtttttatc ccctgctttt ccacctgccc actcagaacc gcgtaagtcc tgaatacttg
   121081 caaaatagat gaaaggtttt tcggtatcaa caaggttttg aatcttttct ccatcttgtt
   121141 ttgaaccata gatgtacgaa ccatctgtga agattttttt gaaatccgta aaccaaccat
   121201 cacttacaac aggtcgatga gtcatgataa gaactttttt gtattggctc tctttaatca
   121261 cttgaagaga agaaagcgtc ttaccaaaac gcatcttggc attccaaagc atctcatctt
   121321 tcgttttgaa tacagtctta gtctgctcaa tagcatcttt ttgttctggt ctaaaattaa
   121381 ttttgaactc gtcaaagtca tctttttctg agtcaagact acctttacca tctttaaccg
   121441 cttggattgc tttcttggca acgtcaactg ttacactgaa ccactcatta ggcttccctg
   121501 tgtcatcttc gccaatcgtt tgaggattat agcccgaacg ctttaatact tcatgcacgt
   121561 catggtcacg gaaaaattgg ttcttcttat taacagccaa ttgcgcccaa tgaaggacaa
   121621 aaggtacccc cgctgttgtc atgtactgct ttattcttct acttgcatac tctcttaaat
   121681 actcgctatt aggagtaaag tctgcctcat aattctctgg tactgatgca tccccaatct
   121741 tttccagtcc ttggtacatt ttccatgtgc cgtcagcatc cttggcactc tggatgtaaa
   121801 taagtttacg gtcaaaggtg ttataggtga taacaaacat ctcatccgta atttcttcct
   121861 ttgggtttaa cgatttatcc ccagacagat tgcgtgcaaa gactcttagc aaggtatcaa
   121921 gagtaactaa agattcgacc gcctctgaaa caggtataat tccattttca tgtgctgcaa
   121981 catttccata ttttctcaac ttatgaaata agtcagctat cgtaggagta atattatttc
   122041 tgaccctaag ctcgcgaagt acatcaacat acttcatact ttgctggtca atacccacat
   122101 ttgaagcaat cttccgtgct gctacctccg aataacgtct agttccaata gcaacttcat
   122161 taggtatttg attaacggtt acacggtacg tgttaattgc atctactaaa ttaggaatcc
   122221 caaggaagtg ttctagctta gaaccatatt taggtaagta atcgaagtat ttagctttag
   122281 atttcaacgc tgtcgtcatc aatttcctcc atctcttcct tataaacctc agttatcggc
   122341 atcgctacat aacgtaatat tggatcattt gaaacttctt catcgacctc gtcatcaaat
   122401 gaaaacaaat cgagctgttc aaatacaggc agagctgagt atgcctctcc aaactctcgt
   122461 gttacccctg aataaatatc ctccaaagaa tattcagttc gatttacagt gatttttttc
   122521 tgtccttttc ttagcgtctt aggtgtccac tcactaaaaa taatcggatt atcatctggt
   122581 gttcgttttg taagaaaatc tccctgtttg atattcctag aaatcaaagt ctttgcactg
   122641 tcttggactt tccttttcat tgcggtacca tgttttttcg attgttctct gtacaaatcg
   122701 ttgaatactt gaaacatgtt cattacacag gtctgagcat tgtcttcgag taactccacg
   122761 ccatatagag ttgaaagcgc cagtaaggag taattttcat actgttctag ggactcatta
   122821 tattcacgag caaccattgc catttttcta gtcaaaacct caactaagaa agcaccttct
   122881 ccagctgaag gctctaagaa agtcgtctgc agctcctcgc aagcctcttt tatgcctggc
   122941 aaatccaaca tatctttgac agttctttgc ggtgtaaata cctcaccgtg cttctggaca
   123001 cgttttttac ttttgattag tcgtccttgt tcctcttcaa ccatcaccat atcttcaacc
   123061 actaaaaatc acccatcttc ccaacacaaa taaccgaaac ggatgctacg aatcggttat
   123121 cactttcata tatgggcttt tgggcagact gctggctagt actgtgattg cgtgtcatca
   123181 tgcccttttc ctccttacaa gtcaagattt ttacttgaaa aaactcgaca atttctaatt
   123241 atgcttatgt tcaattttaa cacatttttg ttttttcctc tatctcaaaa taaagaacaa
   123301 aatatagctt aactggagaa ttatagagtt tgcctcttgc aacacagggg agttagcgaa
   123361 gatgttcgtc tgtcctattt tatttttatg aacatgaaaa aagaccacca aactaggcag
   123421 tctttgactt attattattt acttttagct attcccatta aattttacca ctcgtccctt
   123481 tttcgccctc tttagattta gccgagggtc gtgtggtaaa agctcattat tgtcttaacg
   123541 tggtcaaaaa cctctttcct acgtttattt gataacccat actgacgaaa cacctctttc
   123601 attatcgcaa tcatttgctc attagataac ttttcatcat acccatcaat gaagttctct
   123661 aatgactgac tatctaaagc tttctttatt tcaaagaaca agtcataaac aggtttatct
   123721 tgcttcttca gccagtgaac atagccctcc ctgtgctctt tttcccgttc acctgttact
   123781 ccttgctgtt taggtaatca cagaatgttg accgactgac ttctatacct gtatcttctt
   123841 atattctcga taaagacttc ttaatggtga gtccttcttg ataatacttc agcccagcct
   123901 gtaaaacttc gtctggaaac ctctttcgat gttctgtctt gttcgctgaa aaactcttgt
   123961 actcatcatc aaagctgagt gtaaatacca caacaagatg ttcgtacgat agtctgacca
   124021 gaaaactgac ttccttgtcg tttgtaccca aattttcata tgaagtagat aaaagaactg
   124081 tatacctgga aaagacgtct tcttttacga agtcaatgca caatagacgt agaataggct
   124141 atcttcttcg acagttagtg aacctatatc gtctgctaat aggatatccg actttaaatc
   124201 taaatatttt tcctctgatt ttttaatttc cctcttgaaa taatctattg taatttgttt
   124261 atctgaattg tttgcaatta acccctgcag aacttctcta gcttgttcaa catcccgtat
   124321 taccatggaa atcactaggt atcaaactga gtcatacgaa gacaaaaaca taaaaattga
   124381 cctgttaaag ggtcactgaa taacatataa aatcaataaa acggagtcag acacggagtc
   124441 aaaaaacgct gagagtaaaa aatatacagt cccataggta aagtgccaca ggactgtata
   124501 tttttttatt tcagattacc tgaaatgatt ccatttactt tttatctcat ttttccctac
   124561 tagttagtat caaatactct cctattttct caataaacta ttttgggtta catggtatct
   124621 gaaacagcta aaaccttgtc attacagcaa aaaatcactt tatctccgtt tcatttcaga
   124681 tacatttcac ttaagcggat tcccaaaaaa taaaagaaaa agaaacccct agttagccta
   124741 tgctttctaa tgattcctct cgaaacacgg tcaaaaaaca acggggtagg gtagttaata
   124801 aaaagataga aaaaccatta ttcaatgact ttctcaaata tttcgttgta atcagttacc
   124861 tttccgttct taaataccac agaataatct ttttttccat tttcaagtaa cctaagtgga
   124921 atatctttac cgacttgctc tgacaccgca ttactgactc ctgttagatt agctaataaa
   124981 tctgataaag attgagcatc agtcggaatc tctgtatcag tatttatcgt tattccctta
   125041 tcttcattta caagtttcca agtaggtggt atagccgcgt atgtttttat gaacgtatca
   125101 atttttttat ctacagtttg tcttgatatt gtatcgtttt tcttttctac cttaccaatt
   125161 ggactctcat ctttcgtagt cctataaaga gtggctgttg ataagtgttc atccaaattt
   125221 accaacacag ttaatttatc tttttcaccg tcaataggca tgaacgttat attgcttatt
   125281 cccgtggttt cctttaattc tggaatcgct ttaactaatt catcttttga tatcatgatt
   125341 gaacgaaatg tagttgaatc aaatttttta tgtcttagag tatacttaaa atccaccatt
   125401 tcaccatttt tttgtagttc tattactctg tctttagtta ttttaaaatt atagtctaca
   125461 ggaattttct cagcagttcc tgaccaatat ccatagaaat ctttatcttt atttctacaa
   125521 cctgataatt gaataaccag tatagttcct ataaccaaaa atatacctgt ttttaaataa
   125581 ttctttttca tcataaagct cctcttttaa agcatttaat caacaatgta agaacccaat
   125641 ttttaaagct ctttaaaaac aatttccatc aattattata acattatttt tctcatctat
   125701 attagtattt tatttacagg gaatcaaaaa aactccttag taagcttaca cctttaggag
   125761 ttcctgtttg gacggtatac ataatacatt taaatcaatt taacattaac acctcagtat
   125821 atggtatctc tacatataca cggtctttct ttggatagta ctcgacattt atattgatag
   125881 cacttccatt gtctaacgta gctttagccc ggtatgtgtt atctccattt tcatgagtaa
   125941 tcgtcacatc atcacatgac ctatttaagt tgtactgttc ttgtaaaatc tgtgtcacca
   126001 atggtttaga tgacttctct aatccagatg tacctgtcat ttttgaaaaa atgataccta
   126061 taatagccaa gacaacgata cctataatga caaacttctt gttcatattt actttattca
   126121 actttttatc tcctatctcc aaaataattt gctcttatcc ctcttatcag tacattgaca
   126181 tccctaaaag tacaccaaag attgctccag ctacccctgc tatcatcaaa atcgtaccca
   126241 ttttctcatc tgtttctcga tataagtagc ccataataac agcaacagca ccaaatacaa
   126301 caggaataaa tgctaacgat actaccatag aaacccaacc aataatcaaa tacagcttcc
   126361 ctgtctcttg tccatgtacg atttgttcct tcatttcact aaatgtctgc tcagtacttt
   126421 tctttacttc gtctgctcca gttgtctcat ctgtttcttg taatgctccg caaaactggc
   126481 aatacttact ttcatctgtt atttctttac cacattttct acaatacatg tcttctcctc
   126541 ctaaaatcat tcaattgctt aatagttaac tgtttacttt attaaacaca tcagaaaaca
   126601 gctcgtttat tggtacactt aatatctcag aaagcatttt catttgatag ggtttaggaa
   126661 tcgttctccc tgtttgccac gaacttacac attgctgtgt aacattcatt tttttggcta
   126721 actccgtttg agaaagtcct ttttcttttc tgtatcggct gagattactt acttttttca
   126781 tgattctccc ctcctgtttt attatataca acaatatgat gtatttcaac tcataaaata
   126841 taaaaaactt caaataccat tacaaataca acaacttgtt gtactatact gagatggggg
   126901 tgtattaatg aaacagaaac aatatgcgaa ggtcattaga gaaaagcgaa aagctctcgg
   126961 gtggacacaa aaagaattag ctgaaaaaat cttctctaca caacaggcag tagcaagatg
   127021 ggaaaattct gtaacagagc ctaacctcga tagcttaaca gctctttcta gagccctagg
   127081 aacccctgta agccattttc tagacaatgt tgtcgtagat tatgaggaag agtttttggc
   127141 tctctatcgt tctcttagca ctgaagatgc agttcgaaca attgactata tgaagctatt
   127201 aaaaaggcaa gaaaatgaac gcaatcagct attgaaagat tgaaaaataa ataacccacc
   127261 gaactttaac ggtgggtgta ttgagatgaa ataagattaa gagctatgga taacctgtct
   127321 agtttaatac aattgggtct tttctcttcc ttactctccg tccatcatct cttgttacac
   127381 ttgtaaattg atacagagga actatcccag ctcctgataa tatcaattct tttattgttt
   127441 catctatccc tgcccctttc gctaaatata ttaaaaaact actacttgct ttcacattgt
   127501 atagtttctt aatatcctta gaagctcgtt caagagtgaa tccttgttta acaaagtagc
   127561 ctctgttttt tattaaagca tttttcacgc tgttatcccc aacattacgt aggcgttcac
   127621 aaacagtgtt aataagttct ttctctgtca ttagatatgt cccttgccac gataagccct
   127681 gtaaaacttc atgtgcttca tctaaaatca attgtttttc cttgctgagt tctgatcggg
   127741 aattttgagg aaatactctt ttagcctctt tttcgttaaa acatatctta acccattcat
   127801 aagaaaaata ttgatatgta aaatgaattt tttccagaag tggaaggata tcttcattca
   127861 atttttgaat cacagaagtt gcattaaaaa cgtctaatcg atagacattg cttctcttat
   127921 gattaaagtt tcctaacgct ttctcttcct ttaacctatt taggtattcg agcatcttca
   127981 aaggaacatc ttcatcatct aattttttta tcaatcctat aaaagtcata agtgatagta
   128041 acttattaca tttatctatg tcataagttt taccatagta agaactcatt tttttagcta
   128101 attgttcccc cgacaaaata caagtcacac tttccttact atcatcaaaa aaattaacat
   128161 cagaaagcaa gacatcatag acacgtcgca tttcctctag ataattcctt attgtcctga
   128221 aaagtttgtc tttcattgat tcaagagtgt caaaaagtac ttttgattcc tctttcaatt
   128281 ttcgaaaatg tgtaggatac aagtctaggt tctctaatag ccaattaaat gtttctaccc
   128341 gactgtcttt ctttaaaatt agcatcacaa ctccaataaa attttcactg atatcacaat
   128401 tactggaatg acaataaaaa atggaatgcc cagttttact ggtaaaaata ctagcagagg
   128461 gattatgatc gttgtgaaac agacatctta agtttttact tgacttaagt aatttattca
   128521 tatcaatggt taacaatctt tcataaatct cattaaaaga catgtcggag gaatcaaaca
   128581 ggcagtcttt aaaccactgt ttcatttctt ctttgtcgtc attttttatc aacttaacaa
   128641 aatcggtttc attgactagt tcatactctc tccctgaatc actttgacaa tttttagaac
   128701 atgtcatggt aaaatcaatc ggaagctcag taatatctaa catgttgtct tcattaatta
   128761 ttatcactgc atctgactta tatcctccaa aaaacattct attcgaagca tttacattgt
   128821 catcacaccc ggggatgttc tttttcaagt attcgtaaac agcaaccatt ttaccatagt
   128881 cagtaattgc ttcttttaat agaaagacaa tcttgtattt ttctagattt tcagagtacc
   128941 gaatactttt ttgtatcgca aacgcagaat tatgaactaa attgatagat aaaacctttt
   129001 ctacgcttaa gtatctacta tcatctttac tcaaatctac taccttcccc ttctcatctt
   129061 taacagtgtt atcgacatcc agaataatta ctctggtttc cacaaaactc tccttgtcat
   129121 tgcctttatt ctttgtcttg cttggaataa atgattgtga tgaatttagt atctctacaa
   129181 actcatctct tgtaacttct ctatagttat ctaaacacct atctcttaca tcagtttttt
   129241 ctttttttgt aactggcttc tctaataatt tttcttgatc tacataaatt ggaatattaa
   129301 tcattgatgt ttccatttgt gtttcctcct tataaaattc tgttaaagac taaaacatac
   129361 agataataaa cacaaactct ttattactat atgttttagt cttatttgtt tactcaacct
   129421 ttatactgtt ccatgaaact atcaatatca gattttttga atctaatgac cgtatcaatt
   129481 tttataacag gtaacccgta ttgagaaaca tattttctaa tcgttcctac tgatacacct
   129541 agatattcac ttgcctgctg atagtccata tactctagag taacaccaga aataatgtct
   129601 tctagcatag gctttagcac tgtaattatc tctttcactt gttgttcaac ttgtatagaa
   129661 acaatgctat caatgatttg tagagacata tttccccctc ctttcgtgat tacttgaaca
   129721 aattttctaa gaatcatctt cgtattattt gatcaaaata aaagtatttt gttgttatta
   129781 accgattaat caaataaaaa atgattatca gttcatcgac atacacataa tacctcgata
   129841 caaaaggggt gtcaatatgt tgtgtgaatt tatttacaac accccctttt tgaggtatac
   129901 taattttagg aggtgtggtc aatatgataa agcgtccacc aataaattat ttagaacgaa
   129961 aaaagatact cggaacaaaa ataaaagcta taagaaagag taaaaaatta acacaaccag
   130021 catttggtct tatgattaac aatggtcaat tgattgacaa aaaaactatc tacgaatggg
   130081 aaaagggaac ctatttacca ataccagagc gattatcccg aattgctgat ttgggaaata
   130141 tgagtataga ggagttagtt tgtggcaatg tagaagaata tattttggga attattcttt
   130201 atagagatag cattgtacta gatggtataa catttccaga taaaaacctg ttccagcatc
   130261 ttcgtcaaca gttcccacca gtacactcta atttagatac atggctggat agatattcca
   130321 aattagaacc agagatgcaa gaatttattg ctaacaagac ttgtaataaa gttaaaaatg
   130381 aaaaaattag tcttttcaat atcttaaaaa tagaagaatt attcatcaat gctattatag
   130441 aagaatttga caataacatt cttctgctca ctagcagtat agaggaacaa ctagaagaaa
   130501 tgatagatga agggatacat agtcaagtgg aagacatgaa ttatcccgaa gaagctgtaa
   130561 acgaaattat tgataatata aacaagctaa aacaaacgat ttcatcaatt gggaaaaaat
   130621 acactaagaa aaaaatgaag ggaggtgata ccatctagtg gtaagtactc cttgtaaact
   130681 aagaggagat gatttcatgg caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc
   130741 gtggatgtat aaaggacaca ttgccaccga tccacgaact ggtgaaaaaa taaatactac
   130801 gagaagaggt tttagtagca aacaagaagc tcaaaaagat tatgacgagt acagacatca
   130861 aatattatac ggtgttaaaa agaaagcacc tgatatgaca tttgaagacc tgtataatga
   130921 atggataact catcagcgta ccagtgttaa agcttcaaca atcgcaatct cagtaagata
   130981 cgcaaataat caaatacttc cagcatttgg taagcttaaa ctatcgaata tctctgtccc
   131041 atattgtcaa aaagtggtag atgaatggca cagtaaatat gaaagttacg attatatgag
   131101 aaaacagact gcacaaattt tacgctacgg ggttgcaatg caatatattg acaataatcc
   131161 tatggaaaaa acacttcttc cacgaaaaaa agagtatgag aaaaatcgta aattttactc
   131221 aaaagaggag cttaataatt tactggatgc ttttaaagat tttggtaaca tgaaacaata
   131281 cgccttcttc agattattgg catacacagg tatgagaaag agtgaagtac tcgctttaca
   131341 gtggaaagac atagacacgt ttaataaaga actacatgtc aataaaactc ttgctgtaga
   131401 tgaatttggt aaagttataa ttcaatctcc gaaaacaaga gcttctagac gagttatctc
   131461 tttagatact gagactcttt ctatcctcaa caactggaaa ttacagcaaa aagaagagta
   131521 tttaaaactt ggatacaata ctagctctaa agagcaacat gtttttacaa cagttaaaaa
   131581 cactctatac attccaaaca ctgttaatga ttggttaaga tatattttga aaaaatataa
   131641 cttgcctcgt attacacctc atgggtttag acatacacat gctagtttat tattagaagc
   131701 aggagaatct gtcaaggtag ttcaacaacg attagggcat gaaaatagta aagtgacatt
   131761 agacatctat gcacatatta caaataatgc acctaagaaa acaggtcaag actttgctga
   131821 tatgatggct catcagtaaa agagctggtc aaaagctggt catttctagt atatagacaa
   131881 aataaaaaag ctagaaccct tatataccaa ggattctagc tcctataaca agtttatcct
   131941 acactgcctt ccatctcata actgatcaat cggttgagtt cgactgcata ttccattgga
   132001 agttctttgg tgaatggttc aacaaagccc atgacgatca tttcagttgc ttcagattcg
   132061 gtcaggcctc ggctcatcag atagtagagt tgttcttctg agatttttga gactttggct
   132121 tcgtgttcta aagagacttg gctgttgtgg atctcattga atgggatcgt atcggatttc
   132181 gaccattcgt ccatgatgat cgtgtcacat tcgatatgag agatcgaacc cgcactgttt
   132241 ttggcaaagg ttacttgtcc tcgatagttt acttctccgc cgtcttttga aattgattta
   132301 gagacgatag agctagatgt atttggtgca ttatggatca tttttgcacc cgtatcttgg
   132361 atttggtttg ctcctgcaaa ggcgatggag agcatggtgc ctcgtgcacc ttttccatct
   132421 aagtaaacac ttgggtattt catcgttgtt tttgcaccta agtttccgtc gatccattca
   132481 acggttgcgc cttcgtatgc ttttgctcgt ttggttacta agttgtacac attgtctgac
   132541 cagttttgga tcgtggtgta acggcaatac gcatcttttc ttgtaaagat ttctacgatg
   132601 gcagcatgca gactattact tgaataagta ggtgccgtac aaccttcgac atagtggatg
   132661 cttgctcctt cgtcaacaat gatcagcgta cgttcgaatt gccccgtgtt ttccgcattg
   132721 atccggaagt acgtttgtaa tggaacatcg actttgacgc cttttggcac gtaaataaag
   132781 gtgccacctg accatacggc tgaattcagc gctgctaatt tgttgtcggt tggcggtaca
   132841 agttttgaaa agtactcttt gaataattca gggtattctt tcaaagcaga gtcagtgtca
   132901 gtaaagacga ttcctaactt ttcgaattca tctttcatat tatggtacac gacttctgat
   132961 tcatattgtg cggaagcacc ggctagataa gctcgttcag cttctggtat accaatcttt
   133021 tcaaatgtat ctttgatttt ttctggtacg tcttcccagt cacgagcggg tttgtcactt
   133081 ggtttttggt aatatttgat ggcatcgaaa tcgatgtctg aaaggtcggg cccccatgtt
   133141 tgcattggca ttttattgaa tgtttctaga gatttcagac ggaattcaag catccattct
   133201 ggttcgcctt tgattcttga catctcacgg atcacttctt ctgtcaagcc ttctcctgta
   133261 ctgaaaaccg gttcgacatc atcgtgaaac ccgaatttat attcttctaa ttctggtacg
   133321 cccatgttct cactcctctt ccttgtggta atgatggaaa gctgcggtac catcgttttc
   133381 cattgctttt cctagtgctt tccaggcaag tgtcgcacat ttgatccgag cgggaaattt
   133441 agctacacca ccaagcatag cagcatcgcc taattggtcg ggatctttga cttcttttcc
   133501 ttggacaagc tgtagaaagt ctacagttaa atgttctgct tcttctattg attttccaat
   133561 gacggcatct gtcatcatcg aggcacttgc tgtactgatg gaacagccgc tgccgtcaaa
   133621 ggcgatattt ttgatgattc catcttttac atcaacttgc aattcgatca catctccaca
   133681 tgtggggttg ttcaattcga tcttgcttgt cgagtcagtc aagcttccac gatgatgtgg
   133741 atgggaagaa tgatccaaga tcacttgacg gtataaattg tctaatttag atagtcccat
   133801 gtttgaaaaa ctcctttgtt gccatgattg cttcaactaa acggtcggcg tcttgcgttg
   133861 tgttgtagaa ataaaagctg gcgcgagcag tcgctgatac ttcaaggtac ttcaatagcg
   133921 gttgcgcaca atgatgacct gctctaacgg ctaccccttc catgtctaaa gctgtcgcga
   133981 catcatgggg atgtaaccca tccaaattga acgcaatgac tcctgtccga aaattcgggt
   134041 cttgcggacc ataaacagtc aatccatcga ttgcttgtaa ttttggcaac acataagcgg
   134101 ctagttcttc ttcatatcga tggatattgt ccatgccgag ttcttgcaaa taatcgattg
   134161 catgacctaa tgcgatagcg ccagcgatat ttggtgtccc tgcttcgaat ttccaaggta
   134221 gttctttcca tgtactgtcg tataattcga caaaatcgat catttcgcca ccaaattcaa
   134281 caggttccat ttcttctaac cattttcgtt taccatataa aacaccgata cctgttggtc
   134341 cgcacatttt gtgtccgctg aaagcaaaga aatcacagtc tagtgcttga acatcaatgg
   134401 tcatgtgcgg ggcagattga gcaccatcta ctacgatgac cccgccgttt tcatggacaa
   134461 gttcagctaa ttcttgaaca ggattgatga cacctaagac attggataca tgagcaatgg
   134521 agacgatttt ggttttgtcc gtaatctttt tcgctgcatc ctccatatcc aggaagcctt
   134581 cttctgttag ttcgatatat ttcaagacag cacctgttcg ttttgccaac tgctgccacg
   134641 ggataacatt agaatgatgt tccatgtaag agagcactat ctcgtctcct gctttgatat
   134701 agcggtcgcc gaagcttctg gctacccaat tcaagcttgt cgtcgtacca cgagtaaaca
   134761 gcacttctgc tgtttctttt gcatggatga acgcccgtac tttttctctt gcttgttcat
   134821 aatcatgggt cgcgcgttct gctaacgtat ggacaccgcg atgaacattg gcgttatcgt
   134881 gttcgtaata gtgcgctaat gcctggagta cttgttttgg cttttgggtc gtcgctgcat
   134941 tatccagata aactaaaggt tcatcattga cgtcttgaaa taagatagga aaatcattgc
   135001 gtagcttttt agcatcgatc atccattcaa cttcccttcg attacttcga ctaattcttt
   135061 ccgaacttgt tctacaggga ttgctgttaa gacagaaccg aggaatcctc gaatcactaa
   135121 gcgctctgct tcttctttat gcaatcctcg actcatcaga tagtacattt cttctggatc
   135181 aactcgtcct acactggcag catgtccagc agttacttcg ttttcatcga tcaaaagaat
   135241 aggattcgca tcaccacgtg ctttatcaga aagcatcaag acacgacttt cttgttgggc
   135301 atctgcacct tttgcccctt tcaagatgtg gccgatacca ttaaaggtca atgttccttt
   135361 ttctcggatc actccgtgtt gcaaaatatg cccgatcgta tgaggggctt tattggttac
   135421 tcgcgtatcg attccttgga tttgtctgcc cgagctgatt gccacgattt tgacttctgc
   135481 gtgtgcacct tctcctgcta aatctgagtc aaagtcagcg atcacatgac cgtcattcat
   135541 gacaccaatt gcccagtcga cagaagcatc tcgtaaaatg tgcccacggc gattcatata
   135601 agaagtaata ttttctccta attgatctac ggctgaatat ttgatttttg aaccagcttt
   135661 agcgatgact tccacgatga tattacctga tgattttgct acttgttctt tggttgtctg
   135721 gaaacgttcc agataagaaa attcactatg ttcatctgcc acgatcaaca catgcttgaa
   135781 gaagtgctca tcggatgcgc catcttgaat gaataaggac tcgagtggtt catcgatcac
   135841 gacattttta ggcacataca ggaaaatacc gctgttcata aaagctacat ggagtgcagt
   135901 caattggtct tcgttcatgt ttactgcttt tttcatataa tattcttgga ctagttctgg
   135961 atattcgatc attgcagtga acaagtcggt aaaaatgact cctttttctg ctaattccac
   136021 aggcagttgt tcaaaaatcg tccatgaacc ttgttggaca agcacaggat tgtctttcat
   136081 tgcatcaaat gccggaatcg tgcctgtttc actgatggtt tgtgtttctt tcacatcaaa
   136141 taacgaccag cgatggaatt tcacccgatc gatatgcggc aatggcagtt catctgcttt
   136201 cgctagtgcg tcttgacgga aggttctcat ccaatcaggt tcgcctttac ttaaagaaaa
   136261 atcatttacc gcgtcaagat aatcgagcca gtttttttct ttcattcttg ctccccctta
   136321 cgcttcttct tctttatagt cgattcctaa ttctttactg ataccagcgt agccttctgc
   136381 ttccagacgt ttagctaggt cagcattccc tgtcatgact actcggcctt ccatcatgat
   136441 gtgaacgaca tcaggtgtga tatagttcaa taatcgttga tagtgggtga tgatcaatgc
   136501 accgaaattg tctccgcgca tttcatttac tcctttagca acgactttca aagcatcgat
   136561 atccagacct gaatcgattt cgtctaaaat tgcaaatgtt ggttctaaca tcaacaattg
   136621 caggatctca ttccgtttct tttctccgcc agaaaaacct tcgttcagat aacgttcagc
   136681 catttcttct ggcatattta gtaaagccat tttttcatct aactttttca aaaatgccat
   136741 aacagaaatt ttgtcgtttt catcacgttt cgcattgatc gctgcacgca tgaattccgc
   136801 attcgtgata cctggtatct cacttggata ttgcatagct aaaaataagc caagtcgcgc
   136861 acgttcatct acttccaagt ctaaaacatt tttcccgtca aacaaaattt cgccttctgt
   136921 tacttcatag tttggattgc ccatgatagc ggcagataag gtagattttc ctgtgccgtt
   136981 aggtcccatg atggcatgga tttctccggt tttcattgtt aggtttacgc ctttaaggat
   137041 ttctttttcc tcgatcgaca catgtagatt ttttatttct aagacagaca tgcattatac
   137101 ccccaaattc tctttcgttc tcactcattt tagactaatt gagaatgaag cgcaatgacc
   137161 ttccttacta aaatcaaata tcttttattt attattaaac aattttgatt tctaaattag
   137221 aataggtata aaaaagatga gaaataagcc attacagact tatttctcat cttttttgtt
   137281 ccatacctta ttttgcatca gcctttactg gtttaacaga gccgccccat ttgtctgtga
   137341 tccattcttg gacttctttg cggtgaagca cttcaatcag ttttttgatt ttgtcactgt
   137401 cttcatcacc tttacgagtc gcaataatat tcacgtaagg agagttgtcc ttttcaagca
   137461 atacggcatc ttctaatgga ttcaaccctg cgccgtaagc gaagtttgcg ttgattgcaa
   137521 ctaggtcacc ttcatcattt tggtacgtag aagctaaaag tgatgggtcg atcgtatggt
   137581 tgaacttcaa gttcttaggg ttttcttcaa tatcatcgaa tgttgctgtt tccagatcta
   137641 ctccatcttt gactttgact aagccagcat cttgtagaat tgtgatgatg cgtccccagt
   137701 ctgattcaga atttgaagta atgattgttg caccatcttt taattcggaa acatctttga
   137761 cttttttaga gtacaagccc attggttcga tgtggacagc accggcattg acaaagtcat
   137821 atcctttttc tttgatttct ttgttcaaat aaggaatatg ctggaagtaa ttggcatcaa
   137881 tgtcgccttc ttccaaagat tggttcggta atacatagtc atcgaatttg acgatttcaa
   137941 gatcgattcc ttcatctttc aataatggtt tcacatgttc caaaatctct gcgtgcggtg
   138001 tcggtgaagc gccgactttc aatgtcgtgt cgtctgcttt atttgctgaa tctttgctgt
   138061 cgctgttgtt accgcatgct gctaatccta cagtcagcaa aagcgctgct gcaaatccaa
   138121 aaattttctt tttcatatcc tacatccccc tatatcctat tatcttttgt ctgttttctt
   138181 agttaagaaa tcaccgattc cttgaacgat aaatacgatt agtaaaataa ttaaagtcgc
   138241 aacgaatgtc actgtcagat taccacgctg gtagccttcc tgccaagcaa gacctccaag
   138301 tccacctgcg ccgatcgcgc cggccatcgc agtaaatccg atcatcgaga tcgttgtaac
   138361 tgttaatcca gaaatcaatg cgggaatact ttcaggtatc aagaccttcc agatgatttc
   138421 ccagtaactt gctcccattg catctgctgc ttccagtaca ccagggctga cttcacgaaa
   138481 agcaatctct actagacggg cataaaatgg tgctgcggat aaaacaagag ctggaatggc
   138541 tgcttgcgca cctagcatag tgcctacgat tgctttagta aatggaatga tcaaaaccat
   138601 caggatcata aatggcgttg accggaaaat attactgatg atcgatacga ttccataaat
   138661 gattcgagca tacggtttgt ttttgcgtcc aatagagtaa aggatcaatc ccaaaactaa
   138721 tccgatgatc gtcactaaga ccattgagat taatgtcata tatagtgtat caaggatagc
   138781 ctgattcatc gtttccgggt tgatttgttt aaaatctaga tatgtttctg aaaagctttt
   138841 atccacgatg gatcacctca attcctactc cttgttcttt gatgaacact tctgcttgat
   138901 ctaattcttt ggtttcacca gtcatcaaga cagtcaatga accaaaggaa ccttctttca
   138961 cttgtttgat ctttccatac acaacgttga catcgatgtt atagcgtcga atcgcttggg
   139021 aaatgactgg ctcgttggca tttctttcgc taaaggtcaa agttgccaaa cggccgcttg
   139081 gattttcttt gatgagttca gctaatagtt ctgtcgaatc agcttctggt tcgatgtctt
   139141 gttgtacaaa gcgtttggtt acttcttttt tcggttgtct gaagacagtc aatacgtctc
   139201 cttcttctac gactttcccc aactccatca cagcgacttt gttgcagatt ttgcggatca
   139261 cattcatttc atgtgtgatc aagacgatgg tcaaattcaa tttcttattg atcgctaaca
   139321 ataagtctaa tacttcttct gttgtttgcg ggtccaatgc gctagtagcc tcatcacaaa
   139381 gcaggatctc aggatcattt gccaatgctc gtgcgatacc gacgcgttgt ttctgtccgc
   139441 cggaaagttg tgaaggatat gcttgtccgc gtccttctaa cccgactaaa cgcaacagtt
   139501 cttctgcccg ttctttacgt ttgctctttg gtacgccagc taattccagc ggcagctgga
   139561 tattttcaag tacagttctt gaccataaca ggttgaaatg ctggaagatc ataccgattt
   139621 tctttcggaa tgtacgtaat tctttgtttt ttaattttgt aatatcttga tcgtttacga
   139681 tcacttcgcc gctcgtggga agctcaagtc cattcaggag acggactaat gtactttttc
   139741 ctgcgccaga atagccgacg attccataaa tatccccttt ttcaacgttt agtgaaacgt
   139801 cattgacggc ttggatgttt ccgtttttcc cattgaatgt ctttttgaca ttcgttagtt
   139861 ggattaatga catgccattc acatctttct cttatgattt ttcaaacaaa aaagccttcg
   139921 ccctcttgcc ttaagcaaaa ggacgaaagc ttgaacttcc gtgttaccac cttgatttac
   139981 cgttacttcg caataacagc ctcttccagt acttggactt agcttctttc cgtatactgt
   140041 cgcgctgtaa cgggcactcc cgtagtaagt ttgcttacgc tcgcttactc tgctctaaga
   140101 ccatcttcca tctcattttc gttacccatt ttcagcaaac agggctctct tcaaacgtcc
   140161 gtgacatgta ctcttctttt caaagctttc tcgtttcata taggattgct tatgattata
   140221 gcaactggaa gaatcagtgt caactttttt caagtgattt tctgatttta taattcgtca
   140281 aattcttttg gatcaacatc tttgaaactt aagatccacg cattcatttc atctgaatca
   140341 ttcaagatat tgatatcttc accgatttta tcattaacag aagaaactgt acccgttagc
   140401 gggctggcaa attcattgac cgctttttcc gcttcaactt cgatcagagg ttcatctttt
   140461 tttagcgatt gtcctgcttt tggcagattg gcaaacgtga tgtctccaag ttcttcttgc
   140521 gcttcatttg tcaaaccaac acagtattct tttccattaa ataagatcca aaggttgtcc
   140581 tttttcttca aatgcttgcc tgccatttta tatcagttct ccttcataaa ttttttcatt
   140641 gaatccaatt gcaacaagct ggtcctctcg taagatcagt ggccgcttga tcagcatacc
   140701 atctgtcgat aagacttggc tcgcttttga agccgtaaac tcgcctactt gatctttcag
   140761 tccaagttcc cgatatttca tcccgcttgt gttgaacagg cgacggattg gtacttgact
   140821 cgtttctagc caagtctcaa tcgttgaggc cttcggtact tctttgatca tatcaacagt
   140881 ttgataacta acgccatgct ggtctaacca cgctttggct tttttgcatg ttgaacattt
   140941 cgggtaccag taaaaggtgt acaattttct tacctccttg aagatcttgc aggtgcttca
   141001 ttttggtatc tcatcgataa tatcaacccg ataccgatca tgttccctaa aatcgaagat
   141061 cctccctgac taatgaaagg caatgggatc cctgttaaag gaagtaatcc gatatttgct
   141121 ccaatattct caaagacatg gaacagcagc atcatgatca aaccgcttgc gatataggcg
   141181 taaaattcat tattcgtatc gaaacacacc cgaatcatac gatagattaa aataaaatac
   141241 aagaaaatga cgaatgcacc accgataaag ccgaagtttt caccgataac ggtaaaaatc
   141301 atatcggact cacgaacagg cacataaata ttgcttacgt tgaatccttt accaaacaag
   141361 ccaccggtac caattgccaa aagtccttgg gccggctgat aactcattcc tgacgtatca
   141421 tggaaagggt ccagccatga atcaatacgt gcaaactgat aagatttgaa gccaactttg
   141481 taaagcaagt ctcttccgcc ttcagtcgta accaagaaga tcgttcctgc tccaacgact
   141541 actgccaaga ttactacagg tgcgatgatg cgccaagaga ttccagacat caagaaaatc
   141601 ccgccaaaga tcgcaagaaa gacaagcatc gttccaaagt catcttgggc catgatcaat
   141661 gctaaaaccg gtattgtcac gatcaagagc ttcccaatca gccaaaagtc cgatttcatc
   141721 gtccgttctt ttacttgcgt attatgttta gttacgatca gtgccatcat caagatgtac
   141781 gcaatcttca tcaactctgc tggctggaaa gtaaacgcac cgaatcgaaa ccagttttta
   141841 gaccctgttg aagcagctaa attctgatca tagaatttga gcaagagtcc cattaccact
   141901 aaccccaaac cataaaggta aggagtcagt ttccagatcc atttggaatt caaatgcatg
   141961 atgacaacga ttgctgccgc tccaactaaa taccagacaa cttgcatccc tacgcctttc
   142021 aagactgctg gattgttcgg atcatggcta agtgctacat ataatgagag cagaccaatc
   142081 aaacataaca gaaagactgg taaaatcacg ccataatcaa tacgattatc gttcgaagct
   142141 tttaattttt tttccatgtt tcatcccttt tcttaaaaac catactgctg attattatag
   142201 cggtccagtt agataatgga aagtctttcc atagacattt gggaaaaatt aactttccgt
   142261 ttgtgcagtt gcttgagcaa atgcagtaca aaagcctact gcttatcttt agtgtatcat
   142321 agaacttttt ttgatacatg caattatcta ccagctgaga ttctcctcgc tgtgagaagt
   142381 ttctgtgctt tcattgtatc gaaggatctc ttcggcaatc tctagcagct gatcagaaac
   142441 cagattggtt cgttggatga aataaaaata tcgatggaaa ttcaacggag tcattggcac
   142501 attttctgga acagagcgct tagacaaaat cgagcgaccg attccttcct tcagcaacgc
   142561 aacaattaca tcgttacttt tcacgatcat cttcttatct tggatattca tctcttcaaa
   142621 ataacgctgc gtataatgaa aaacaccaga gtttgcttct cttacaagcc acaatttgct
   142681 ttttgggtcc ccagcgatga ctagctggtc ttccatcaaa gagtatcttt gtacgccgga
   142741 agtctctaat ggcttttcga taaaaccaaa atcggattca tgtttcgcta cttgttctaa
   142801 tgattcatga gaattcgcga tttcgatctc aaaatgcact ttaggaaacc ttttgatcag
   142861 atgtttcatc aactgaggca aaagatagac agcaaaggtg tgggaagcaa cgatccgaca
   142921 tacttcttct tcattttgtt cattgagcaa tttttgctga gtttcttccc attcttctaa
   142981 caaaaccagt gtccgttcat ataataagtc ggcttgctta gttggaagca tctcttttct
   143041 tccgttacgt tgaaaaagat cgatattcaa ttcccgctcc aactgtttga tttgattcga
   143101 gactgctggt tgagataaaa acagtacttc tgctgctctg gaaaaatttc ttgtttcata
   143161 tactgctcga aaagtcgtta ataacttaaa catggcacca cttccataac tttttatgat
   143221 aattactata aaaactattt gatttaaata tacaaaaaac catctataat agaaatgtac
   143281 aacaaaacag ttgctgatcc aactagtaac ttagatgaag acaatcagct gtctgcttgc
   143341 tcgtaatcat acattcctat tcattttaca atgtattcgt tcatcttggt actaaaatct
   143401 attaaataaa aaggagaata ttatatatga agaacttttt gaaaattatt ccgatcccga
   143461 tctgcgggtt gatccttggg ttagcttccc ttggaaatct gtttaaagac tatcaccatg
   143521 ttgctttagg caatctaact ggcggtatct ctatgattct tatgatattc atttttgtta
   143581 aattgtttat cttattcgaa cacacgaaac aaacgttgca tgatccaatc atcgcctccg
   143641 tttcaccaac ttttacgatg tcactcatgg ttatttgtac ttattttgtt tcatatgaaa
   143701 gtattgcacc aattgtcaaa ttcatttggc tgtgcgctgt tatcttccaa gtgatccttg
   143761 tcttttattt caattatcac catgttatca aagctgatct ttcaattgaa gcaatttatc
   143821 caagctggtt tatcatttac gttggatttg gggtcatcac tgtaactgcc gggaacttct
   143881 tcccgcttat tggaaaaatt ttcttttgga tttcattgat ctgctatgcg ttcttattgc
   143941 cgatcatcat caaacgtgtc tttttcgtaa gaaatatggc tcatccgaca cgtccgctga
   144001 ttacgatcgt tgcagcacct ggttctcttt gcctaacagg ttatcttaaa aactttgctg
   144061 aacctaattt ttatcttgtc gtagcattgt tcgctttatc tcaagtattg tatttcgttg
   144121 tgctgtctat gcttccacgt ttgttcaagc ttcgttttta tccaagctat gcagccttta
   144181 cattcccgct tgttatctct gcaacagcta tatttactac tgttcattac ttcaacagtt
   144241 taggtatcta tagtagcgta ttggatttct taaaagttgc ggaatgtctg tttgcaagtg
   144301 tagttgtctt ctacgttctt gctcattacg tacgtttctt aattaaagaa catagcaaac
   144361 atggaaaatt tgtttctaaa aaaatcgctt aataatcgat tcaataggtt gtgaaaaagc
   144421 tgttctgcat caagtaataa gaacggccaa acaaaaacag ctgttcatgt ttttgtactg
   144481 gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaccaagag gtcgtaaaag
   144541 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
   144601 aaatttcggc ttatttccga agaagctact tctggaacac cgtttaccaa gaggtcgtga
   144661 aaaagccgct tagtttcgag tattaagaaa aattttgtga aaatggcttt ccacattttt
   144721 gcataaattt ggcttaatac cgagaaactg gcttttgaac accgtttatt cgaaacaaga
   144781 ggtcgtgaca agacttttgt cacgacctct tgttcttttg tcaaaatact ggtttagtgc
   144841 tactatgttc gagtatgctt ttagtattta ttgacgaaag gaaccgatca aatgaaatca
   144901 ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc gcgaaatctt acccggttta
   144961 ctgctttctt ttgccatcgc tgccttcagc aggcttcttg ctatttatct gccacagtta
   145021 ggtggagcga ctattgctat tttattgggt attttattag gaaatacttt ttttaaacag
   145081 cctgttctag caatcggcac aaaatttgct gaaagcaaac tattagaatt ctctgttttt
   145141 ttactaggtg caactgtcac agctcaaacg attgctcaaa ttggtataaa aggaattgtt
   145201 tttgtgttac tgcaaatgtc agtcactatc atcggtgctt acttgattgg taaaaaactg
   145261 caattcagcg atacttcttc tttgatgatg gctggaggaa acgctgtttg tggttcttct
   145321 gctattgctt cgatcgcccc ttccatccat gcgaaagagg aagaaaaagg acagatcatc
   145381 acactagtca atctactggg tactgtgatg atgttgactc tcccttttct tggattacag
   145441 ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg gtggcacact ccaatctgtt
   145501 ggtcaagtag tcgctggggc aagcttactt gacaattctg ttgtcgaatt cgccatgcta
   145561 tttaagattc tacgtattat gatgctcgtt gttgttgtct atctgtttga aaaattcatg
   145621 cacaagaaac aaccagctct gtttgaaacg ccaactgata cgcagaaaaa acgaaaatta
   145681 ccacttcctt ggtatgtatt agggtttgtg atcttttgtt tggtcaacag cttgttttct
   145741 ttacctgtag ttattgacca atcggcacat tttatcagca gctggtttga gatcactgct
   145801 ttagcggcaa ttggtctgcg tttagacttt gcaaaattca tgaaagaagg tacacgcttc
   145861 ttattttatg gattagctgt cgggcttctc caaacagtga tggctttact atttatttgg
   145921 ctgatcaaag tttagaatga acatgaaaaa acagttgtga ttctcgcatg gaacaatgtc
   145981 cgaatcacaa ctgtttttaa ttttgcagat atccttctaa caacaacaat tggcgtttga
   146041 tttcaacccc atctattgcg tttcccatat atcctgtcag ttttccattt tgcccgatca
   146101 ctcgatgaca aggaataacg attggcaatg gattggttcg atttgcctgc ccaatcgctc
   146161 gcacagcttt cggactatca acagcaacgg ctacttcttt atacgttcgg atttctccat
   146221 aaggaatggt cgacaaagct ttccagacat tttgctgaaa aggcgtacct ctctttatag
   146281 ataaaggaac atcaaattct tttctttttc ccgcaaaata ttcttcaaac tgttcttgtg
   146341 tgcgcgttaa aatttgctga ttcgctgatt cgctttctga aattggctga aaagatacag
   146401 cagtcaatcc ggattcatct gcatctaacc ataaaaagcc gataggtgtg tgtattttca
   146461 ttttgtttgc ttctagtcaa tagactggaa cttcctcctt tttctctatc caaaactact
   146521 tgccaactaa taaaaattgt tcttaaaagt ttgataaacc gaggaaacat ggtaaactaa
   146581 atacgttagt ttacaaaaag aatacctgtt cttttacgta aaatcaaatt ttaaaggtgt
   146641 tatttaaggt ttattaaatt ttgaatgtaa accatatata aacatacgat tgaaaggata
   146701 gaaacgatga aagtatcaat tgttgtccca tgttttaatg aagaaaaaac tgttcctctt
   146761 ttttttgatg aggttgaaaa attcaaaggt gcccatgagt tcgaatattt gtttattgac
   146821 gacggatcaa aagacggtac gttagcagca atcaagaagc tagcctcagc ttatccttat
   146881 gtccgctttg tctcattttc cagaaatttt gggaaagaag cggctctata cgctggattg
   146941 caagcttcta caggagattt agtgaccgtg atggatgttg atctgcaaga tccgcccgaa
   147001 ttgcttcctg agatgatcaa ccggatcact acttctgata tcgattgtgt cggcacccgg
   147061 cgttcgactc gtgaaggcga gcctgcgata cgcagtttct ttgcgaaaaa attctaccag
   147121 ttgatcaata aaatcagcga tacagaaatg gtcgatggcg ctcgtgattt ccgcttgatg
   147181 actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat acaaccgatt ttcaaaagga
   147241 ttatttagct gggtcgggtt taaaacagaa tatatttctt atgaaaaccg cgaacgtgtg
   147301 gctggcgaaa catcttggtc cttctggaaa ttattcaact actcgattga tggtatcgtg
   147361 aacttctcgg atgcaccgtt aacgattgct tcatttatcg gcgctttgtc ttgtgtcggt
   147421 tctggtattg caattttgtt cattattttg cgggcattgt tgtttggtga tcctacatca
   147481 ggatggcctt ccatggtctc gatcttcctg ttcattggtg gaatccaatt gttatgtctt
   147541 ggaatcatcg ggaaatatat cgggaaaatc ttcttagaaa cgaaacagcg tcctgtttat
   147601 atcataagag aaacagaaaa agtgaagcca aatgaattga agcctcacca ataatatacg
   147661 tcaaatgaag taaacatttt tttaaatgaa aaaggactgt gacaaaagtc ttgtcacagt
   147721 ccttttttcc gaataaatgg tgttccagaa gtagcttctt cggaaataag ccgaaatttc
   147781 acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc
   147841 tgtcacaacc tctttcatat cttattattt cttcattgcc gcttttagtg ctgctgctaa
   147901 tgggctttct tctggttctt ctgcttgtgc atattttttc agcaagcgtc gttcttcatg
   147961 tttggtcatt ttcttcttct gctctttttt atcaagcatt ttttctgtcg ttccatcgta
   148021 tttacaacgg aaataagcac cgtttttacc ttcgatgatt tccattttcc ggtgacattg
   148081 cgggcatcga tgattagaga cttttggatc ttttcttctg cggtagttgc aatctgaatt
   148141 ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg cgcagattgg ccccacattc
   148201 tgggcatttc ttttgggtaa tcgagaaatc ttgatattct tgtttgcttg atttgatctc
   148261 attcaccaag cgttttgttt ctttttcgat tccattcaag aaggttcctg cttgtttctt
   148321 tcctgcagca atagcttcaa ggtctttttc ccagctttct gtcaaatctg gtgttactaa
   148381 agagggattt accaatacta ataattgctt acctttagga gatacttgca gtccttgagg
   148441 gatacgttcc atcagttcag atttgattag tttctcgata atctctgcac gcgtagctgg
   148501 ggttcccaga ctgtattttt ccatttgacc aagcaagctt gcttctgaca atggtttagg
   148561 cggtgcagat agttcttttt tgatggcgaa atcgggattg attctcattc ctttttccca
   148621 tttgactgtt tccgtctcag tttcagtttc gcctttccat cctgcttgga cgacacgatt
   148681 ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta acttttgttt ggctgacttt
   148741 atggggttga gcaaacagtc ctaaaaaacg agagacaatc atttgataga ttttttgctc
   148801 ttcattactt aatttttcat aacgtgctct gttttcagtt ggcaaaagtg catggtgatc
   148861 ggtcacttta ctgtcttgga atacttttgt ctgttggacg atggctccgt ttttcaaata
   148921 ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat cgttctttca tcgtactttt
   148981 catgtcattt gtcagatatt tgctgtctgt acgcggataa gtaacgatct tatgggtctc
   149041 atacaaactt tgtacaagat ttaatgtttt ctttgcagaa tagccatagc gttgattggc
   149101 ttctctttgg atctctgtta aatcataagg caatggcgca gattccgttt ttaatttttc
   149161 ttggacttct gtgacagttc ctttgcttgt actgatcaat tgaacaagtt tttgtgcttc
   149221 ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt ttggcttgtt cttttccagc
   149281 ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc tcgattttct tttcttggtc
   149341 tctcactaat gctagtgtcg gtgtctgcac acggcctgct gataaattat ccgcgtactt
   149401 cacagttaag gctctggtca cattcaatcc taccagccag tccgcttttg cccgggcaag
   149461 agcagattca tacaatgcat catattcttt cgcaggtctt agttgcttga atccagtttt
   149521 gattgcttta tctgtttgag aagagatcca taaccgcttc actggtttat tgaaatgcac
   149581 atactctaag atccagcgag caacaagttc cccttctctt ccggcatctg tcgcaatgac
   149641 tgcttctgag acatcttttc gctgagctag ctgcttgatt gcttttaatt gatgtcctgt
   149701 tttaggaagt ggtttgattc ctagtttttt agggatcatt ggcaacgtgt ccatttgcca
   149761 gctctgccat tccttattca gatcttccgg cattttcaat cctaataaat gaccaagggc
   149821 ccaagtaacg atcaccttgg gtccttcata ataatttttg tgcttttgat tagcaccaag
   149881 caccttgctt aggtctttcg caacgcttgg tttttccgct agaattagtt gtttcataaa
   149941 attcctttcc gctttctact gtgatgggct gatgattttt caacaacgca agtcctttgt
   150001 attgctgtaa atcatcgtca cactcttcac accagcgctc atctccgtca ttccaaaagg
   150061 cgctgagaaa gttacttttt gcattgatat attcataatt ttgatggatt tcgttccatt
   150121 gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa tgtttcttct gcttcaatat
   150181 cttcttccca gtcttcaaaa aaccaccaag gctcattgtc cccgaacatg gtaattactt
   150241 gatacatact cgtgctcctt ctttcagctt ttctagactc atcttagtca cttttcaaga
   150301 aagttgcaag aattttctct tataaaaggg tcatttaatg aacatcttaa aattgttaca
   150361 aatgaatctg attgtttaag gcaaagtagc gataaagtca ttgatcatcc aaaaatagct
   150421 gtcttctgga tcgattgttg gttcttttgt aaagtatcct aaaaaacgta aggaaagata
   150481 accgtggatc atactacgaa gttccctgct tcgatgaact ttttcttctt tcgttaattg
   150541 aaaagcctca aatatctgca aaatgatctg attcgtttca tggattccat ctaataaaac
   150601 agcttcattg gtatgcggta cactgatcag taactcatac actgcttgat ttgaaaaagc
   150661 aaaggatcga taagtttctg catagatctg cagtgcttcc tgaccacttt ttcctacaag
   150721 agcccgacgc agctggtcat taagttcctg cagaagtttc gctgttagcg ctgttttgac
   150781 atcacgaata tttttgaaat ggttatacag cgagggagcc ttgatatcca agcgtttggc
   150841 taaatgctga aaggttattt gctgaacatc catctcttct gctagttctc gaaagcagtc
   150901 gatgattttc tcttgagaca gatttctttt tttcatttcc atgtttcctc tcataaaata
   150961 aatccttcac tctttcagtt taccacgaaa aagaaagaga aaaaagagac tttcattgat
   151021 aaactacata ttgtagttta taataaagat ctgtagatga aaattttcag aaagaaggag
   151081 tctaattggc tgcaagaata aaggaaaaat tatttaggaa aagagaaact tgggaacaaa
   151141 acttgtttgt cttatggttt ggaacattta tggctggtat cggatttagt ttagtcatgc
   151201 cttttatgtc actttacata gatacgttag gtaattatag tacgtcacaa ttgaactttt
   151261 ggagcggatt gactttttca tctactttct tggtgactac gctgatctct ccttggtggg
   151321 gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt gcgtgcttca ttaggaatgg
   151381 caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta tcaattagtc gcattgcgtc
   151441 tgcttcaagg gattttttct ggttatatca gtaatgccac tgcccttgtc gcaactggga
   151501 cgccaaaaga aaaaagtggt caagttcttg gaaccttagc aactggttct gttacaggaa
   151561 cattacttgg accgcttctt ggcgggatct ctgcttcagc gtttggctat cgccctacct
   151621 ttttcattac agggatcatc ttatttttcg tcttcttgtt gagtctgttt ttcgtccatg
   151681 aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc caaacagatt ttcaaagact
   151741 tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac gatgatcatc caagcatcca
   151801 acaattccat tagtccaatc atcagtctgt acattcgcca attgctgcat ggacatggga
   151861 atgttacgtt gatcagcgga atcatcgctt cgatacctgg gattgccact ttgatagccg
   151921 ctccaagatt tggtcgctta ggtgacaaga tcgggagtga aagaatactt gcgattggtt
   151981 tgggttttgc gattctggtt tatatcccaa tgtcattcgt cacgaatgtt tggcaattag
   152041 ctgccttacg ttttttgatt ggtatctctg atgcttgtct gctccctgct gtccaagcat
   152101 tgatcacaaa atattcgcct catcaagctg ctggacggat cttcagttat aaccaatctt
   152161 tccaagcaac tggtaacgta gtagggccta tgatcggctc cagtgtttcc agtgcatttg
   152221 gttatcgtgg cgtttttctt tcgacttctc ttcttgtttt ggtcaacttc ttgcttgtac
   152281 gacacaatac acaagaaatc catcagcaag aaactgcaga agaaaaagct tcaggagtct
   152341 ccatatcttc agggcactct ccttctcata gacaataagc gtaaatgaaa aacactgctt
   152401 gaacagttgt cacagactgt ccttgcagtg tttttgttta ggcctgtttt ttcaagaact
   152461 cagataagag gaataatccc ttttccaagg tttctttttt acaacagtaa cctagtcggg
   152521 catgcttcgg taaatcaaag gcacttcctg gtactaatag tactcctgtt tcttctaata
   152581 aattgataca aaatgtctgg tcatcttctt cgatggctaa tttaatgaaa gaagtagaga
   152641 cgtaattggg agcgacaagt tcaactttcg gttcattggc tacccattgt tttaaaatca
   152701 caaggttctc tgtaatgatc tttttgtttc gctccaatat tttttcttta ttcttcaatg
   152761 catgtacagc aagatcatct gacaggactc cgccgcaaat catcgtgtag tctcgatact
   152821 ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca gccaatacga atacctggaa
   152881 tggagtaggt tttggatagg gaattggttg ctataccttt ttcatagaca tctacgatcg
   152941 acaagaattc acctttatcc gttaaaggcg catatacttc atctatcaat acatatgcat
   153001 ctactgtacg agcaatttct gcgatttttt ctaaagtttt ccgatctagc aatgtgcctg
   153061 taggattgtt tgcactattc aaacagatga tttttgtttc aggtttgact aaggcttcta
   153121 gctgttctaa atcaaactgc caatcttctt cttccttcaa atgaacaaag tcaacagcgg
   153181 cacctaaaga tttggggatg tcatataatt gctgataagt aggaagcatc gaaacaacat
   153241 ggtctcctgc ttccactaac gcgtatagag ctaaaagatt agcaccagtc gctccattcg
   153301 tttgtaagat gttttccgga ttgacggttt gataaagaga agcgacttct tgtttgaaaa
   153361 catctgatcc ttcgatccag ccatagtcca atggttcgtt tttgcgtttt tcgaaaaatt
   153421 cacttacgct agtcccatct aagccaacga gttcgtctaa tgtcaatgct tcgatcgagc
   153481 tttgtgcaat atcatatacc gctttctttt cccatttatt tagccattct tctacaccga
   153541 aatttgcgat tttcatgttt ctttccccct tatatcttga tccagcctac agcaagcaac
   153601 ccgattgaaa gcagtgcggc cacacaaatc agcccaatca ttactttttc ttttcccgta
   153661 atttgatgtt tggtttcacg acaagcgatc cagtagaagc aaaaaccagg aagataggca
   153721 atactacata aaaggacata agaaaatcct gctaaaatga tgcccatcaa ttcaaaaact
   153781 acagcaatcc caccgatcac tacttgtttt ttgattcctt cttttaaaga aaactggact
   153841 tgatagatcc cgacaagtag ataacaaatc aaaattgccg ctgtacataa agaataggca
   153901 aattgatagg cataatcagt aactaagaaa gtcaataaga atagattagt caagattgca
   153961 gtaacaacta acgaataagt tggggcattt tttttattcg tttttcccca acctttcggc
   154021 aacagaccgt ctcgagccat caacatcgtt gtttctgctg ggagcatcgt ccaagacaac
   154081 caagaaccaa tgatcgaaat aatcaagccg atattgatca gcgctgctcc ccaatttccc
   154141 acgattcctt ttaaaatatt tgccatagca ggttgtgaaa tagttgctaa ctcttcttga
   154201 gataagacgc cgtatggcaa gatcgaagca aggatataga taaacaataa acaaagcaga
   154261 ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg cgcggttggc taaaacactt
   154321 gcgccttcga tccctacaaa gacccacatc ataaccatca aacaaccttt aatctgcaac
   154381 cagatcgttc cttgctgccc tgttccattc actagattat cagatacgtt accccaaaaa
   154441 tcagcagtga atatattcac tttaaatgcg ataaatacaa atattaaaaa taggaataat
   154501 ggcactaatt tacagatcgt cacgattgta ttgatgacgc tcgctccttc aactccattg
   154561 tttactaata aagtcagacc ccaaataaaa atacttgcaa ataaaataga aggaacattt
   154621 tgtccacctt tgaatatcgg gaaaaattct cccatggcgc tcatcagcat cgttgcaaag
   154681 gcaacatttc ccagccacgc agacaaccag taaccccaac cactgatgaa gccacctaat
   154741 ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat ctagatctgg acgtttttct
   154801 cctaaatgat tcagcgataa taccaaagct aaaataccaa tcccaacgat tatccatgaa
   154861 aggatcgctg gccctggagc tgcagaacta gccagatcac ttgtgatccc aaacacaccg
   154921 cttccgattg acgaactgac aaccagagca accagtcccc atttgctaat acccgtcgat
   154981 ttcttttcca tttccatcac cctcacttat aaaattgaac aaatcacttg caaaagattc
   155041 ccttctattt tgcaactgct cttgcttaac aaaaagcaac tttattcata ttattggata
   155101 ttatgaactt tttacacaag tatttcaagt ataaaatgaa taaaaattaa ttttattgca
   155161 aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt tgttagccat agctgttttg
   155221 attcatttaa tatcgcttaa acgtattgta aaacttttgt ttcttgatat gcgtggtcga
   155281 tcaaaccacc acccagacat tccattccat cataaaatac aactgcttgt ccaggagtga
   155341 ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt atcatccaat aagcgaactg
   155401 tcacaggaac gtcttgctga cgataacgga atttcgctgt acacttgaat tcttttggca
   155461 ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa gcttgtggca tacaatgcag
   155521 gatgatggaa cccttgcccc acatataatg tgtttgtcgc tagatctttt cctacaacaa
   155581 accagggttc ttgcgaatct cctccgccac cgatccctag tccttgacgt tgaccaatcg
   155641 tataatacat caagccagca tgttgtcctt tgacttcacc atcaagagtg accatatctc
   155701 ctttttttgc tggcagatag ttgctcaaaa attgtttgaa gttcttttca ccaataaagc
   155761 agattcccgt tgaatctttt ttcttagcag tcgcaaggcc tgcgcgttca gcaattgccc
   155821 gtacttctga tttttccatg ccgcctagag ggaacatcgt ttttgaaagc tgttcttgtg
   155881 ataattggct aaggaagtaa gtttgatctt tattattgtc tactccacga agcatgtgga
   155941 ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc agtagctaca taatcggcgc
   156001 caagatccat tgcataatcc aagaatgctt tgaatttgat ttctttgttg cacatcacgt
   156061 ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa atattcaaat acacgatccc
   156121 aatattcttt ttcaaaattc acagagtaat aaggaatccc gatttgagca gctactttcg
   156181 ctacatcctt ataatcttcc gtggccgtgc atacaccgtt ttcgtccgtg tcatcccaat
   156241 ttttcatgaa tattccgatc acgtcgtatc cttgttcctt taacaaaagt gctgttacag
   156301 atgagtcgac accgccgctc atccctacga caacgcgtgt tttgctgttg tctgtcatga
   156361 tatcaccatc atttcaatta gattctgaaa catctacgag ttgaaggtcg caacttttca
   156421 gtattttaca ttatacgtca tatttctgaa aatttcatcc cttaattatc aagtcgtaaa
   156481 aatactgttc tactggacct attcagtaca caaacatgat agtcaaccaa aaaatcactc
   156541 atcttttgag cattcgatat aaaaaattgg catactcata aaaagggcca gcctttcggc
   156601 aataaggaat aattttgtaa attgtgaaaa actatttttc aaaattgctt cttatcgctt
   156661 aaagctgcac gtctcattta caaccttttc tttttagatg ttatgagttg ctcagataag
   156721 tgattttgtt tactctaaga aaaaagggag gcaagagata catgaattac tttaccagtg
   156781 atatgcactt tttccatgaa cgcttattag gaaaaaacga ctttgctccg cggctctttg
   156841 caaatgtgga tgaaatgaac caacagttga tcactagctg gaatgcagta ataaaagaaa
   156901 ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc gcaatatgaa aaagcaaatg
   156961 cagaaatttt ggaactgatc actcaactgc aaggaacaat ccacttgatc aaagggaacc
   157021 atgacagtcg tgcttttttc cgttatttag aaaaaaatga tcctaggctt actgacggaa
   157081 gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa attcgaccat caacaatatt
   157141 acttgactca ttatccgatg ttgctcgggc ctaacgacac tatccgcaat cttcatggac
   157201 atatccatca ttacagcgta cctatcgaga acgatgtcaa tgtaggcgta gacgcaccgg
   157261 aaagagaact cttaaaggaa aaactaccat ttggcactcc gttatctgag caacaaatca
   157321 aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg aaaaaactgc cctatatcaa
   157381 gtattaaaca aataaagata attactcata tttttcattt gagtaggctt attgccacag
   157441 gaacattagg atcagaaatg gagttgtaac aagacttctg tcacaactcc attccatact
   157501 attcgtcttt ttgctcatgg tgttctttta ccaatttttt ctgtaattct ttatcataat
   157561 ttcgagcagc tgacgttaaa aataaaacga ttccgcataa taaaatcccg attccagaaa
   157621 acaagaacat tttttctact cctaaacgat cagctaatgg tcctgcaaat aataatccaa
   157681 ttggacctgc caggttcatc aaagagttga agttccctaa gacacgacct aaaacattcg
   157741 attcataact ctgctggatc attgccatca gcagtgtatt gaaatatggc gttgcacaac
   157801 ctgctcctgc attcaagata agaaagtaca agaacccttg actatcgttt ggaaccaatc
   157861 cgcttgcacc gatcgtcagg cccataatga tgtaggctat aataataggt ttcattcggt
   157921 ctttccatgt tcctataaaa ctgatcagtg caccgcctaa gagcattccg actgcgtaga
   157981 ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa gtaatctagc gtcatcaatg
   158041 gatacagact gattgctggc atgaacaaca gcatcacaaa tgcaccattg atcgttatgt
   158101 accataaccc tttgttatcc atcaattgct gcagcccaaa ttttgcatct ttaaaaatag
   158161 taagcggaac ttccaatatt tcaggagaaa cttttgggat cttcacaaaa atcaataagc
   158221 cgacaccaaa aactgccccc aatacatcta ataaaataag gtaattgaca ggaacgactg
   158281 aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt cgctgactgg accattccta
   158341 actgtccatt cgtccttgtt atgtgagaag atggaacgat tgtcggcata atcgactgga
   158401 tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa caaagacaca aaaactagcc
   158461 aaatcgggaa ggattctgaa atcgttccga ctactgctaa aataagcgcg aaaatcgcta
   158521 caataatatc cgtgacaatc aaaagagctt ttttatccca gcgatccact agcggtccta
   158581 caaatgggct tagtaaaacc atgggaatca ttcctagtaa agtagcaaaa ctcaaaattg
   158641 tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc atattggacg accatactag
   158701 tgattcctga caaaaactgt cccgataaaa agaacaaata atttctcttc caattcgtta
   158761 aaacgaccga ctgttcattt tccataaaca tccctctttt ctttttcatt ttgtatagcg
   158821 tttgcacaac aatcattata cgggattctt ttttcaaaaa ataccttttt ccctaaataa
   158881 caatcgtttc aatttaaatt tctttactta gaaacaataa atcagcaaaa tattaaaaat
   158941 aacaacatgt gttaaattta aaaataaaaa agacaactaa tttgcgtcgt ccttacaaag
   159001 ttagtcatct aacaataggc cgtttcaaaa ctagttgtct ttttatatcc acagatcatt
   159061 cgactttttc gaataaccca cggctaatca tcccatccat cagaaaataa aatttatctt
   159121 ggctcatttt ctgttttgga tctttgaaaa tattgactgc tttcaatcca tttgctgtag
   159181 tgatcgacat tttcaatgtt tgaatatctt ttgatacagt gatttttaat ttgaatcctt
   159241 ctttgctttg cggcgtggca tctaatggtc ggatcaattg ataattgcct ccgtttgttt
   159301 ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc aggatgcaaa cgatagctaa
   159361 ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat gattttcacc tcgataaaat
   159421 tagtttctct ttattataaa agtttatcct gatttttact aggtttttca gttaagttcc
   159481 ctaaaaaatg aaggcgttct ctcagacatc tgttcatata agaatgactt cactagttat
   159541 tttttgacag cacgaactaa ttgatcaagg aaatactgga tgtcgtcttc ggtatttccc
   159601 aaaccaaagc tgatacggat ggattctttt agtatcggtg tatcttctcc atacattgct
   159661 tctaatacgt gtgaaggctc gatatttcct gctgtacaag ctgaaccagt agaaatagcg
   159721 accccattaa gatcaagctt catcagtagc aaatcatttg cttcgccaat taagcgaaga
   159781 ttcaacacat gagggagttt attcgttaaa tcaccattta cttgataagg gatttcgttt
   159841 gcttctaatg tatcaagaat caattgctgg aatcctttat atttgttgtt acgtgcttca
   159901 cgttcttcag atgacagcag ttcaactgct tttgccatac cccagatccc tgctaaattt
   159961 tctgtacctg cacgccgctt ttcttcctgc tccccgccta aaagcaatgc agggatattt
   160021 actccgtctc gtttatataa gaaaccgacg ccttttggcc cattgatttt atgagcagag
   160081 atacttaata aatcgattcc taattcttct ggatggatcg tttggtttcc gtacgcttgt
   160141 accgcatctg tatggaaata agctgggtga tccgctaaaa gttcaccgat ttctttgatt
   160201 ggcatcaaat ttccgatttc attgttcccg tacatcacag agaccaagat cgtatcttct
   160261 cgtaacgctt ttttgacctc ttcgatcgac agattccctt tttcatctac aggcagatat
   160321 gtaacgtcaa acccctttgt ctctaaatat ttcatggtat taagaacagc aggatgttcg
   160381 atagctgtag tgattagatg tctgccttct ttttgacgcg agaacgctgt ttctaagata
   160441 gctgtattat ccccttctgt tccaccacta ttaaagatga tctcgtgagg ttttgcttgc
   160501 agactgtctg caatgatttg tcttgctgtt tccaattttt catgcgcgtg gcgaccaaat
   160561 ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga ttgccgtcat cgcttcaatt
   160621 acttgtggat gcatgggggt agttgccgca tgatccagat aaatagtttt cacttcattc
   160681 atcccttcca aattattgct aggcacattc tagcataaaa tagcgaacaa accaaagaag
   160741 agctcatttt tgtctagagg acgtttcatg ggcatagaaa aaaggagctg tgacaaaagt
   160801 cttgtcgcag ctccttgaag ctgctcactt gtgtcacaac ttctttccct ctttttttgt
   160861 ttattcgaat tctttttctt ccagacggaa taacggactc atcggtgtat tttcatggat
   160921 gcgtttgacc gcttctccca ttaattctga acatgtaacg attgttaagt tttctggatg
   160981 acgattttct tctgtgaaaa cagaatcagt gatacaaata tctttgattg gtgcattgtc
   161041 taaaatactt ttcgcatgat cggacaataa tccatgtgac gcacaggcat atacttcttt
   161101 tgcgccgttt tcttttaata catttgctgc tcgtgcaagc gttgctcctg tgttcaagat
   161161 atcatctacc atgatacatg tctttccttg aacatcaccg atcacataac caccctcagt
   161221 tgaagcatca tctgcatgat ctacgatggc taatgtgctg ttcaaatatt ctgaaagact
   161281 acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact acgatgtcat ctcccgtcag
   161341 acctaattga cggtaataat gagcaaacaa cggcatcgta aataaattat ctacaggaat
   161401 atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa gtcagtactc gagttgcccc
   161461 tgcttcttcg atcaagttag cgatcaattt cgcagtgatc ggttcatgag gtttcgccgt
   161521 gcggtcttgc ctagcatacc catagtaagg caatacgaca ttgatcgttt tcgcacttgc
   161581 tcgcttcatt gcatcgatca tgatcagtag ttccatatag tagtcattga caggtgcatt
   161641 cgttgattga acgatataca catgatcccc tcgaatactt tcttcgatat tgatggaaat
   161701 ctcgccatca ctaaattgct tcacgacgct tttacctaat tctgttccga acactttcgc
   161761 gattttttca gctaatggac ggttgccatt caaactaaaa atcttcaacg tgtcatcctg
   161821 atacttatta ctcatgtagt cctccccaaa acttctctat tcttcgatag tttaacatat
   161881 ttttccaaga ttttctgtta atttcagcaa attcttaagg aagtgaaact gttccgttac
   161941 tccaactggc aggaatgact ttatgataat cgccccagtt gtcgtgtact ttgtaataac
   162001 gttctcctgt ttcagtttca aaataagcgt acgctgtcac ccaatgattg ccatatgtac
   162061 tgccaagtac tttcaaaata ccaatcatca caggtttgcc ttcacgtata cgttttgtag
   162121 ctcgctgcca tgaacctaga agagttgctc gtgcacgctc atgattgcca ttcttcttta
   162181 aaaaactaga aatccctgta ctaacctgaa aaggaatcgt aggaagtcct agtggttgga
   162241 tctggctacg caatgccttc gtcaaaacca acgaatcagc cgtatttttc ttgcgaatct
   162301 catttggaag catccacccc ttacgatagt cttgataata ggcaaggaga actgctgccg
   162361 cgtaagtacc acaaagatag cctgaggggg tagcccattt cttatacctc aaaaaacgtt
   162421 cttctggcaa tcccacccag caatttgcga gttcctttcc tgaccttttt tcttcgatca
   162481 aaacgggaat atcttctttt ctgttgatcc atctagataa ctcgattggc tcttcattga
   162541 tcagcttcca ttttttacaa gaaagaccct cttgataagt atattttgct ggagaatatt
   162601 ctaccggatg tcctgattga tcgataatca cataactttg atcttcaaaa accagagcta
   162661 aaaaaagcaa tttacttttt cttgactgca gctgctgagt gctggctaat tttttaattt
   162721 ttttatctac tttgaaatat aaaggtttat ccactacttg attcagttgg gatcgcctcc
   162781 tttttttcat tataagtgaa ggattgaaaa aaacaaatca gtttgattgt tcgaattttc
   162841 aattttttat gttctatttg ttaattcctt cattttgtaa gataatgggc acacgaaaca
   162901 aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa caagatttct atgaagcagt
   162961 caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat aaaccagcta caggcggttt
   163021 ccaagattta gttgacggga tcgaccaact tttgatggga gaaacagacg atctgcttgc
   163081 ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc cttgcatatt atcaactggc
   163141 aaacgattat gaccgaagaa atgagttgca tgctcaacca cttcaaccta taatcgaaaa
   163201 agttgaatca ttgcagtctt tcgacgaatt gaatgagcaa tttccagaat ggctgttgga
   163261 tacattacca ttgccattta atctggatgt agatgcagat atgaagaatg cgcaaacgaa
   163321 cgccttcttt gcttatccgc cttctttatt ccttccagat aagacgtatt atgcatcaga
   163381 ccatcccaac ggtgcacaac taataggcgt tttctttgat atggctgttc agttattaga
   163441 gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg gaacaagcag tgcaatttga
   163501 taaattgatt gctcctcatg taaaaagtgc agaagaaaac gcagattaca gcaaaatgta
   163561 taatccgcaa tcttttgata catttattca atacacagat aaactggact tgaaaaaact
   163621 tgtcacgggt ttgattggga caactccaga taaagtgatc gtaactgatc cagtgtactt
   163681 cgatcatttg aatgacatcc tgacagaagc tcattttccg ttattcaaaa gctggatgat
   163741 cattaaaaca gtacgttctc ttagtggtta tctatcagaa gaattccgcc aagtcagcgg
   163801 aatctattct cgcgtattgt ctggaacaga cgaagcaatg cctcagaaaa aagcagcgta
   163861 ttaccttgcc tctggacaat tcgatcaagt agttggcgat tactacggaa aaaaatattt
   163921 tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa aaaatgatcg ccgtttataa
   163981 aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca aaagaaaaag ccgtcataaa
   164041 attaaataac ttaggtatcc aagtaggcta ccccgataag atcccagcat tattcgacca
   164101 gttcaaaaca gtacctgcag atcagggtgg tactttgctt tcaaatactc ttgttttcag
   164161 caaactcaca ttaaaagatc gtttcagcaa atggaacaaa ccagtggatc gttcagaatg
   164221 ggaaatgagt gctgacacgg tcaatgctta ttatcatcca tttcggaatg tgatcgtctt
   164281 tccggcagcg atccttcaag caccatttta cagtctagaa caatcaagct ctgctaatta
   164341 tggtggaatc ggagctgtta tcgctcatga gatttcccat gcatttgata ataacggcgc
   164401 tttatttgac gaatatggca acttgaacaa ctggtggaca gaagaagatc ttacacaatt
   164461 tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt ctgccttttg ctgacggaaa
   164521 agtaaacgga aaattaaccg tttcggaaaa tattgctgat gcaggcggtc taagctgtgc
   164581 attggaagca gcaaaaacgg aatcagatct ttctatcgag gaattttttg tcaattgggc
   164641 aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa cttttactgc aaatcgatgt
   164701 ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa aaccttgacg aattctatga
   164761 aacatttggt atcacggaac aagacgctat gtatctagcg ccagaaaaaa gagtgcgaat
   164821 ctggtaagag gttgtgaaaa agccgcttag tttcaagtag taagaaaaat tttgtgaaaa
   164881 tagctttcca catttttgca taaatttgac ttactactga gaaactggct tttgaacacc
   164941 gtttatcgag aggttgtgaa atcagcgtcc tgacctgagt attagagaaa aaaacggaaa
   165001 aatagttctt catattttgc cgtttgttga gctaataccg agggtcagct gattgaacac
   165061 cgtttagtgg agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca
   165121 aaagcagcaa ggagaacctc atctataaaa agatagaagg aagtgagaca tctattggtc
   165181 tcatttcctt ctatttttac tttcctcagt caattgtttt acatcataaa aggagaaatt
   165241 ccacttattt ttttcgctgt ttcatgacat aaacaactac accgattaac aaaattcctc
   165301 cagctaatgc tagcaacggg ttattaaatt ctcccgtttt tgggagtcct tgagtgccat
   165361 ttttttcact ttcttttgct gtcctatccg gatgagaagg ttttccattt ggttttccat
   165421 ttggttttcc atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg
   165481 ggggcacatc ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat
   165541 gagcagacca tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt
   165601 ctccttttga aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg
   165661 tttcaacgat ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt
   165721 gatttggtgt gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt
   165781 ctgcattctc cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg
   165841 gttttgtctg atttggttct tgtacgattg catattctgg atactccgaa gacggtgagg
   165901 tttctccgtc aaaagtagca cctacagata gtggaagacc atctactctt cccttttctg
   165961 tttgacgatt ctccggtttg atttgcatca atccatggac atggatgctt ccattcgcat
   166021 agcgagtaac aggctgctgc ccttcttgcg aatgtgtatt tagagattcg atccaatctt
   166081 ttgatacggt atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt
   166141 tcaacgagtg atagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct
   166201 caagattttc tgttcggaaa gataagatac cagaagcatg gaagtctgtg tttcctgccg
   166261 aagaggtata aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga
   166321 taatatctgt tccgctattg gaatcgattc cttttgcaag attttcgaaa gacaaactat
   166381 tgatcaattc atgagggccc gataaagagg aatcacccat tttgaatcca ttaccgttcc
   166441 cttctccttc aactcccggt acccaaccgt ttcgataagc aactgagttt ttgatgacta
   166501 ctttcccgat tgaacctgtt tcatttttag caaaaagatc ccaaccatca tctgcattat
   166561 gataagcgat acaaccgtca aatacattcc cttctcctat tgtcagtttg gccgcaaatc
   166621 catcggcatc ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt
   166681 tatgcgctgg ccatgcttcg aaaggatcat ttgagctacc actgatttgg attcctgtat
   166741 ttccattccg ataagcatgg atctcttcaa tcaaattatg gtttccagaa atctgcaatc
   166801 ctttttgcat agcggcacta tttgtaacgt caaacccacg taaaacccag taatgactaa
   166861 acagtgtcat cccatttcca gttcctttaa aatcaagtac aggtcgcttg ttttctggat
   166921 ttttctctgc aatcaattga atcgggtttt ctcttgtccc gttaactcct tttggaatcg
   166981 tcaatcctct ttcaaaggaa taagttccag gagccaggac gattgtctgc ccagcatatg
   167041 cgtatctcaa tgcttgcgtc agagataacg gattttgtct gcttccgttt cctgtatctg
   167101 acccttccgg tgttacataa actgtcgaat agttaggttt acggtagtca actgtatgca
   167161 gaatctcttt tgtttcataa ctagccatct ctacgtattc ttctggcgga tagtttttat
   167221 ctggtgtaaa actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa
   167281 attcccgatt tgcagtgatt gccaagtctt ttgcgttctc aagtacctct cctgttgctt
   167341 gatttttgat tgttaataat ccgtcagcat ttgctctaaa ggacatagta tggtcttggt
   167401 ttcctgaatg attactagag gtcacagtgt aaatcggatc aatcaagcga gtaagtcttt
   167461 cttgcccagg aggatctgtc gcaggatccg agtaagcaac ttcgattcct tctaccttga
   167521 tcgtcatatt ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata
   167581 atttttccca gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat
   167641 aatatgcctc taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt
   167701 tcgtggccgg aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt
   167761 tttccaagaa ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag
   167821 ctggcgcagc ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cctaatccaa
   167881 gtcgcatcgt atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga
   167941 catattcgat ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca
   168001 aatgatcttt tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat
   168061 aggtccaaga ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg
   168121 cttgataata ataggtcatt ccatctggtc cgcttgactg gaattttccg ccgttattcg
   168181 tggaagagag ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc
   168241 tttctccgaa aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct
   168301 tttcattttc tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac
   168361 tagttcttgg tacttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt
   168421 cgttcacaga ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac
   168481 tgagcataag gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta
   168541 ttcctcccgt aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg
   168601 tcaataaaaa acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt
   168661 tgttgataat aaagagattt attaacggaa aaagaataag tttttttaaa ataacgtata
   168721 aaaaagagca taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt
   168781 tattttatca aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat
   168841 gtctcaatgc ccattcatgt ccaacgggat cgaaacttgc cacggaatct ttgaaaatat
   168901 ggagtttgta tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac
   168961 atacgccggt caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg
   169021 ttccactaaa agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat
   169081 agtcttggta taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat
   169141 tatgcggagg aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa
   169201 tggcaaaaac gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt
   169261 tttcaattac ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat
   169321 atgtgtagtc aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac
   169381 aaattcgatc accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct
   169441 ttgtgtcgcc gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt
   169501 gaactgacga gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg
   169561 gaaattcgtt aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac
   169621 gatcaatcct tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg
   169681 caaatatgtg cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc
   169741 aaatcctgtt gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac
   169801 ttgtaagcct tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta
   169861 ttgttggatc aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac
   169921 gacgatcact tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca
   169981 tgataatggt tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat
   170041 aataccgatt ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac
   170101 cacacataac attaggataa caataataag agatttcatc aaaaaagagt tccatttggt
   170161 cgtcttttcc cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt
   170221 acgataaaag ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg
   170281 tttttttaat gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat
   170341 aagcggtcct ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta
   170401 ttgaatcgca taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc
   170461 ctcttcaaga gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt
   170521 ctcgaccttc tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg
   170581 tgaaagatac atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc
   170641 taattctcta gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc
   170701 tggaatctcc aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg
   170761 atcttgttcg tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt
   170821 cgaagttcct ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct
   170881 atagatttct ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc
   170941 tcctgctttc tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact
   171001 ccttattagt ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt
   171061 agtccgatac tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa
   171121 agaattcaat actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc
   171181 cgtggcttct agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg
   171241 ttctaggact tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca
   171301 ggaaaaaggg aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg
   171361 aaaaaagcgg tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa
   171421 aaaatgagat caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg
   171481 atctccgcca tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac
   171541 caaaccgact gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt
   171601 tgacaaataa gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa
   171661 aactagaaga aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc
   171721 aaaaattcgt tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga
   171781 gcttaaagcg tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa
   171841 agaagactcg gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag
   171901 acattcatgt aagaatcgag aacagattca acaaggagga attttttatg gatcataaac
   171961 cgactgaaac aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc
   172021 cgttttcaac tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc
   172081 aagagcaaca gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag
   172141 ctaaacagct agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat
   172201 cggctgccca agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac
   172261 aagatattgg tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca
   172321 atcctgacga actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca
   172381 aacaatcgat ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga
   172441 tttcagtgaa attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac
   172501 agctttacca gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag
   172561 aattgaaaat ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag
   172621 aaactggcga tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc
   172681 tggataaacg tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac
   172741 aaatccgttt gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg
   172801 ctacagccat tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa
   172861 aagatgcagt aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa
   172921 attcagaaat gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg
   172981 tggatatcga gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat
   173041 tgcgtatcca aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca
   173101 tggaagaaga tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt
   173161 tactaaaaca aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct
   173221 ttcttctaac ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata
   173281 gccaaaataa atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt
   173341 ttgcagacga agcatcaata caatacaagt caagatggag acgattggca atagcggaac
   173401 gaatggcact ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa
   173461 gcctagacta acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac
   173521 attcaatggg aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt
   173581 tttcggtgta cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt
   173641 gctgacagca aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag
   173701 cgttaaaatt gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat
   173761 tgcaaaagcc actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat
   173821 cagcgtcacg atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg
   173881 cacatctttt tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa
   173941 gaaagcaaaa aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa
   174001 tggctgccag ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat
   174061 accaaatttg acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga
   174121 cacgatgtaa gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt
   174181 ttccggattg tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa
   174241 accttgcata tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc
   174301 gatcaacaac caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc
   174361 atatggccca ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa
   174421 aatgataacg attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt
   174481 ggccgcaacc cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat
   174541 gatcaaatca gacgttttta gttctttttt catctcactc gtatgattga gattgatctc
   174601 tttcttacga aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta
   174661 aactgttttc atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat
   174721 aacataaatg agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt
   174781 agagataatg atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac
   174841 tgttagaagc atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca
   174901 cacaaactga tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc
   174961 gatactttga ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt
   175021 atatacacca taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag
   175081 cattttatta tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc
   175141 gcttacaaga agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc
   175201 atggaagaaa acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg
   175261 tagccctaca gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact
   175321 ccaagctaag ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac
   175381 aacgaaaaag acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg
   175441 attggcatcg ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg
   175501 gactaatgct attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt
   175561 atcagcgaaa acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt
   175621 tttcattata aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag
   175681 ttgattcagc cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc
   175741 ctaatcctgt tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa
   175801 agatccgttt caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga
   175861 ctccttgcgc ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga
   175921 tggcattact aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag
   175981 tatgatcctc accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac
   176041 tgcgtatcac aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg
   176101 agaaactgtc gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata
   176161 attcattttc tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta
   176221 gcaatttaga agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt
   176281 tttggtcttg ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct
   176341 gattgatata ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt
   176401 gcaaagggga agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt
   176461 aaccacccag cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg
   176521 tgctcaaatc aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa
   176581 aaaacagcca tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct
   176641 taaccgctca tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc
   176701 ttggatacct gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt
   176761 gtcatctaca aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat
   176821 ctttccattg tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat
   176881 gatctctccg ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct
   176941 catcatttca ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt
   177001 tgcaatcagt acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat
   177061 agccatgatc atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga
   177121 tacttcacgt attttttgac accaataata gccatccaaa acgggaagat taatatctag
   177181 taatacaagc tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt
   177241 tccaaaggtt tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc
   177301 ttcgtcttcg actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac
   177361 tacaatatca aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc
   177421 aaacgttaaa aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt
   177481 aaatttatta tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt
   177541 ttgtctttta aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc
   177601 tagaaggagt ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga
   177661 gacttcttct gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt
   177721 gtaaggtgta caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt
   177781 tgtttttgtt ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata
   177841 cacttcaaga tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt
   177901 ctcagcaata ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa
   177961 agaggtgcct tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa
   178021 taaaggcaag ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta
   178081 atattctttt cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg
   178141 tcccattcct gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc
   178201 taattgttgc tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat
   178261 ccttctttga tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc
   178321 agctccggca agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg
   178381 aggtctatta cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc
   178441 aacctttata aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt
   178501 tagatttacg gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg
   178561 ccaagagccc gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt
   178621 ctggccctgg attttctact cctacgatct gaatttcttc attatagctt ccataagaga
   178681 tgataaattc agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg
   178741 tctctttcat ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat
   178801 ctgcgacaaa ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt
   178861 cataaacagc taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat
   178921 tccacaattc gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa
   178981 actttcttcc acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt
   179041 catcatttcg tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt
   179101 ctgtggaaac cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc
   179161 tacctaaagt atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc
   179221 cgttgcttac tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc
   179281 gcacttctat ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt
   179341 aatttttgat aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg
   179401 ctgtgatcgt gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt
   179461 gttctccagc ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct
   179521 gtttatctgc ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt
   179581 ctttccatgt agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca
   179641 taacaaccat tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa
   179701 gatagacttt atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg
   179761 cttctccagc ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc
   179821 aactttctgt gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt
   179881 ctcgttccgg tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa
   179941 acaaatcaaa atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc
   180001 tattcggtcg ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt
   180061 cacttggttt ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta
   180121 ttcccgcagt aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt
   180181 ttttctcccc gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt
   180241 aaagaaatca acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga
   180301 cctggattgc ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg
   180361 ttataaacac gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat
   180421 tcagccatct cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa
   180481 aattcaggga tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca
   180541 ccttttaatt cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca
   180601 aggccctctt tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct
   180661 gctggtttga tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc
   180721 cccccttcta tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca
   180781 gttgttcctc tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt
   180841 ttaggataga tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc
   180901 aaaatagcag caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga
   180961 tcaatcgatt cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct
   181021 ttgatacgtg caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa
   181081 gtccctattt ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt
   181141 tcagcgattt gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga
   181201 taataatcgg tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa
   181261 atgatatctg ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat
   181321 tcaaattgat cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg
   181381 acatcttccc ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt
   181441 agtaaaacaa gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct
   181501 ttttagattg tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag
   181561 caagcaggat atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat
   181621 cgttcccgac actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact
   181681 cttgctgtag atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta
   181741 gtgtacccgc ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat
   181801 aactaatttc taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt
   181861 ccttgatccg atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg
   181921 gatctggtgc caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa
   181981 ttgaaaattc tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca
   182041 aatcgattgg cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat
   182101 agccttccgg caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac
   182161 cttcttcatt cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga
   182221 cagattccga gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat
   182281 cgacatactt ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt
   182341 tcgattcttc tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat
   182401 cccccacttt cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt
   182461 ttctaactcg gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac
   182521 gatcctcttc atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga
   182581 gttggatagt ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag
   182641 gttctttgaa accttctggg ttctggctct caatgatttt gtattctgca tagttggtag
   182701 tttcttcact atccttataa acaaatgaat ctggctctcc gattggatga ataactttct
   182761 caccatccac gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt
   182821 tctcttcctc tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca
   182881 gtccgttacc atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc
   182941 cttcaattag tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt
   183001 tgacatacaa ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct
   183061 tgtgatattc tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg
   183121 aaccagatgt atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg
   183181 catatttttt gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag
   183241 cgatctctct gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca
   183301 tcttcgtttc aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg
   183361 cttccccact ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc
   183421 cattttgacc cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag
   183481 aagatgggaa atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa
   183541 tataggccat attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta
   183601 cttggtactt gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag
   183661 cttggttttc ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc
   183721 catgacgttc ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat
   183781 ctagatggta aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac
   183841 cagtttcatc ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct
   183901 ccaaggctgt aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat
   183961 cttcttctaa atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc
   184021 tcccgctaac gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt
   184081 gatacttgtt tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt
   184141 gcgaagtagg actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg
   184201 ctttattatc tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg
   184261 gacggttaaa gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc
   184321 cagaaaactc ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca
   184381 tcacagtcca gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg
   184441 cataactgaa ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat
   184501 tttctccgcc attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg
   184561 gagttcctcc gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga
   184621 tatcatttgt caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac
   184681 tattatggta aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat
   184741 ctttactcat ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc
   184801 cagcggttaa ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac
   184861 tgatttcctt tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat
   184921 cttcaggatg tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga
   184981 tgtacgcccc gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct
   185041 gaacggtttg agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag
   185101 catagtcgta atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt
   185161 tctctccgtc atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat
   185221 ttcctaacac ttttgtatag tcattcgttg taaacttcgg gtagcgatgc tgttcgtttt
   185281 caacttctcc gctaggatag atatattcat aggggtcttg cgtagctgtt ggcccaagac
   185341 gctccaaaat accgttgaat aactggcgtt gaatgcgctg gagtgcttga ccgctgggta
   185401 cgattggttc gtccagcttt tccatctcat tttcttcgct acgttgaatt tcagcggaat
   185461 cttctgtatc agtttcattt tcttgtaatt cttggttatc tattctttca tcaaccacca
   185521 cttgaagagg tagttaccct tcgttcacat cggggatctt ttctgtttca aaaatcaatg
   185581 tgccggtttc tgcttctttt gaataaactt ttccgacata ccattcttga ccatcgatcg
   185641 tctcgacttt taggtcttct ccatcttcat cataggagga tagacctgtt ccacgaacgt
   185701 tttttacggt accaatcccg gcggctgccg catcgattgc taaacgtatt ctaccttcat
   185761 tggcactttc ctttttttca aaagccagat gccattcaag ggtttcttct ttttcaatat
   185821 acttcccttc aatttttgca tagtcctcat ctaagaatac ctcatcctcc tttgtttctg
   185881 ttgactcagc agctacatcc agggtcgtca tatgatagac tgactggaaa tatggtccaa
   185941 atagcaaact gacagttact aactgcaaaa gcttcaattt taaccctttc acatccttat
   186001 actcctttca aaaatatttg tcttctttca caacaatcaa caaaacaatg cccatctaat
   186061 tataatttaa tttaatttta attttaaatc aaatgatatc ctttttagtt aaaaacacca
   186121 attatacaac atttattatt aaaattaaat tataaaattt tataacgttc tcatttaaca
   186181 aaacttaagt tattaagaca aatgaataaa caactacgct ttcctcgaaa caaaaaaata
   186241 aagcacaaat gcgcatttac gcactcatgc tttattcgta caattaattc agataacggc
   186301 agcgcatagg atgccaataa cacttcctaa aatagctcct cctaaaatgt cagtaggata
   186361 atgtaaaagt aaaaaaatcc tcgaataagc gatcaaacaa gcaaataaaa aggctaatat
   186421 tcctgcaact ggacttaatt cgaacaacgc agctactgat aaaaaggcta gcgctgtatg
   186481 tcctgaagga aaagaagaag tcttcggttt acttgtgtaa aggacaagcc gacgattttt
   186541 ttcataaggt cttttccgtc ttgtcgtttt tttgataaaa agcaaaccaa caaaaaaata
   186601 gatcaaactg ccgataagaa tggctagacc atattctcta gtatcaggta tcaagactaa
   186661 aacagcagca aggagcaacc aaataaaacc accattagag atattactga taaatttcaa
   186721 aaaattgatt tttcgactat ccgttttccg ttgcttggcg aacttgttga taatactatc
   186781 atcgatttta gcaatcaaac gcaacatgtt attcctcctt tttaagaacc ttttcttgcg
   186841 tacgatggat aaaaagcagg acttgaaaat aaaacaagaa actgaaacct aataaagcaa
   186901 aatagattgg tcctgcaaac cagaatgcga caagaaggat caacgtgcct gccaaataga
   186961 aaagcgcgct tttgaaatca ttaaaaaaca agaaaaatgc agtaatccaa gaagcttctt
   187021 tttgttccag ttgaagcaag attttgtaac taaagagaag cgcgcttaat agaagcaaaa
   187081 acaacgtagt atacatcaat gtatagccaa ttcccccacc ccaatagcgg aaaatctgat
   187141 aatcgatcaa tagaagaaga aaataaacac ttgctaaact atcttgcttt ccataaagag
   187201 agaagttctc acgaaacttt ttccagaata gtgaaccgag ctttttctct tcattttttt
   187261 tgtaatgata aatcatatac agaactgttc gacttgcagt tcctaatgtg aaaacaggca
   187321 agacgcacag gaaccatagt agctgtaaat ggataagacg tggaatccat tcaatgattt
   187381 ctaagaaata atcattggct tttttaccaa tcgatcgatt catcatcctt ttgaacctcc
   187441 tgtcaaatcc atcccttcaa tgaaataacg ttgtgcaaaa aagaacagca aaatcgtcgg
   187501 gatcaagaca agtgttgctc ctgccatcag ataattccat tgtgtcgttg cttggttctg
   187561 aaaaacttgc aagccaatct gtaacgtgta tttttcttga tcttggatat ataacagagg
   187621 acctaagaaa tcattccaag ctgcgttgaa ggaattgatc gcaatggtaa tcaaggcagg
   187681 tttcgtcaat ggaagcatca aatgactcca aatatacaaa tgattcgctc cgtcgatttc
   187741 agcagcttcg atcaattcat tattgatcga taagtaaaat tgacgcatca gaaatatatt
   187801 gaacgcattg ccgaagaacg atggaacgat caggggcaaa taagtaccta cccagccgat
   187861 cttggagaat aatacatatt gagggatcat cgttacaaac cccgggatca tcatcgtaga
   187921 aagaaccaaa gcaaacatca gctttttccc aggaaaatca agcttcgcaa atccataagc
   187981 aataaaagaa tttgaaagga cattaccgat cactactagc acagtgatga ataatgtatt
   188041 tcttgcatag cgccaaaaag gaaaggcggc aatcgtatcc agatagtttt cccatacgat
   188101 tttttctggg aaaaaggtcg gaggataacg aacgatttct tccattggtt tcaatgaagt
   188161 aaacaccatc caaagcaatg gaatgatcaa aatgattcct attactgtca tgaggacata
   188221 acttagtatt ttttttgttt gccgattcac actggtcttt ttgaaataat ccatcatttg
   188281 ccgtcacctc cagaataata gacccactta ggtgcaagct tcaaattgac aaacgtcaga
   188341 accaacacga tcagaaacag gatcattgac atagccatcg cataacccat atcaaaagct
   188401 acgaacgcct tgttccacat atgcagatta taaaaaagca aagaatttgc aggtccgcct
   188461 gttccatttt tcgtcatgac ataagcttct tgaaaaattt ggaaagaacc gatcgtagag
   188521 gtgatcacat cgtaaaaaat aataggagtg attaatggca aagtaatact tttgaacttt
   188581 ccccaaacgc ctgctccatc gatttctgct gattcatata aagatctagg aacattttgt
   188641 aatgtagcta agtaaagtag cattgctcct ccagaactcc atattttcat caaaatcaac
   188701 gcaggtttcg tccattgtgg atcaaacagc caagctggtc cagggatatg gaaccagccc
   188761 agaaatgtgt tgatcaatcc tgcagatgga gaaagcagct gcatccataa gatgtagact
   188821 gctactcctg ataagactgc cggtaaatag aaagctgtac ggaatacact gattcctttg
   188881 attttttgat tgagtactgt ggctaaaaac acactgaaaa tcgtcgtcag cggtacattg
   188941 aagatgacga aataaacggt gttgtataac gatttccaaa ataaatcatc gtgaacgaac
   189001 attcttttga aatttgctag accaatgaaa ttcatcttgg atgtcaaatt ataatcggtg
   189061 aaactaccaa taactgaaaa aagcataggc cctagcataa ataccaagaa cccgataata
   189121 aaaggcgaga taaataaata ggcccatctt gcttcttttt tctttcggat agataaacgc
   189181 ttcaaatttt cttgcctcct ctgtacctag acagtcaaaa atggattatt gattttgctt
   189241 gactaagttt tctactgctt tttgtgcatc tgccaatcct tcttttggtg attttgttcc
   189301 taaaagaatc tggtcaatct gttcattcac taatgtcgtg taatccggag catctaaagg
   189361 aactggtgtg atcaccgtat ttttaaagtt ttcgtccgcc atttgataga tcatttttcc
   189421 tttatccgtt aggttgtcat tattcactaa attctcattg gctgtcttgc tcgccatgat
   189481 atcaaaactt ttttcgccga atttttcttg tacttctgga gaagaaagat atttgatgaa
   189541 ttcataagag gcctcaggat cttttgctcc gtatggtact tccaagacga atccgccacc
   189601 ccacgaccaa tggcctgaac cttcttcttt ttctgggagc tgcatcacgc caaaatcaaa
   189661 atcatccggt gcattttcca tcaaactaga gtaataattg atattttggg cacgcatggc
   189721 tactaatccg gaaataaatg gatcagatac accagaccca aattcggctt ctaatttatt
   189781 gatcgtattt ttcccataat aatcttgcca gtcttggatc cactccaagg cttccacttt
   189841 atttttagta tcaatcttga ctttttcatc tttgtcaaac caactagtcc cgttatctgc
   189901 gtttagcgcc catacatcag cccctaaatt ccataatgga taaaaaccga tgcgtgtaaa
   189961 ttcttttcct tcttttttat ctagtttacg tgctgtttct tctaattcat cccaagttgt
   190021 cggtagatct tgttcactga ttccagcttc tttaaataac tttttattat aaaaaatgac
   190081 ttgtgtatct gtattgaatg ggatcgcata aggatcacct tcatattcta ctgtatccca
   190141 aagctgcgga taaaaatcct tagaagtgtc tttttctaca tatttggaga gattagttgc
   190201 ttgctgtgct tctgctcttt gggcaacgga atttatgtcc tgtacaataa catcgggtgc
   190261 atttccagct gttattgcag ataaagattt agtccagatg tcaccccatg gctgatactt
   190321 ataatttaca tggatctcgt tttgtgattg attgaaatcc tcaatgattt cttcgatagc
   190381 ttcttttcgt gcaccggatc cccaaaagga ccagaaatca atattttttc ttccatcact
   190441 tgttttttca gaagaatttg ctgcacttga actggcatcg cttgcattac ttgatccgca
   190501 tcctcctaat aaaaacaatg ctgaacttgc cagcaaccct gtaaacaata attttttcat
   190561 attttacctc caaattttaa tgtaaccgat gccggtttta atataacgat atcattttgt
   190621 tctgaaaata acaatttata aggtatgatt ctaaaaaaat tattaatgat accaatactt
   190681 tatataattc gcgagcgctt tctttgaatt ttttaatctt tcgtagcaga atgagaatgt
   190741 attaaaaata gattaggagg aattttgatg tttagacacc agaaagaatt gcagtttgaa
   190801 gcaaaaccag atcgtccgaa tccattaatc gcgaaatatc tgcaggagct gatcggcggc
   190861 caatatggcg agatgagtgt agctatgcag tacctgttcc aaggctggag ctgccgagga
   190921 gaagaaaaat ataaagatat gctgatggac attgcaacag aagaaatcgg ccacgtggaa
   190981 atgttggcga caatggttgc tcgtttatta gagggtgcgc ctgttgaaga tcaagaagca
   191041 gcgatgcaaa aagatccaac aatcggtgcc attatctctg gaatgaatcc acagcacgcg
   191101 atcgtagctg gcttaggacc tcgtcctgct gacagcgtgg gcaatccatg gagcggtgcc
   191161 tatatcattg cgagtggcaa cttgttagca gactttagag ctaatttaaa tgccgaatca
   191221 caaggaagac ttcaagtagc acgtatttac gaaatgatcg atgatcgtgg cgtaaaagat
   191281 atgctgtctg ttctccttgc aagagatagc tatcatcaaa atctatggga atgtgcagtg
   191341 aaagaattag aagaaaaaga aggttcaatc ttagtcccaa gtactttccc gcgtgacaaa
   191401 gaacgaaaag acattgctta tgacttctat aacttctcag agggagacca aagtagtgaa
   191461 ggttcttggg ctagtggcaa agcactggat ggcgaaggcg aatatcaatg gttgaaagaa
   191521 cctaagatcg aaggcgctgc accaaaatta gaaccagtaa caccaaaaat gtatgggaca
   191581 cctccattca agaaataaat atctacccgt agaaacaccc gttttttgtt ccacacgctt
   191641 tgtgagcaaa actgactaaa gtcattaatc ataaccaccc ttcaaaaggg tggttttctc
   191701 tagagctata agctcaaatc accggccagc gcctaaagac gctggctttc atattttatg
   191761 ttcaagccat attgcctttg ccagttttgc ccacctctta aaaggtttat gtactactcc
   191821 ttagccctta aaaggatcgt cttactctcg tacactcagc ttatctaacg cactatcatg
   191881 tttttcttgt tcttgaatgt actttttgat ggtcgcttca ttttgtccta cttttgtcac
   191941 atagtatcct tccacccaaa agtgtcgata cccaaattta tattttagat ttgcacaatc
   192001 tcttgatgat ctcctgaata ttggctcgat attgattata aattattttt cttctatact
   192061 ttggtgtgaa tacaatgtgg tatttacaca tctactttgt acgggctaaa ctatttccct
   192121 ttattgctcc acccgcatag caagtggttt tgtatttcaa tcgctcggcg cgctcaatta
   192181 ggtctaaaga caataaatct aaaaacccgc tgcttcactg acagaaacaa cgggttttca
   192241 gaaaaaaatg aaatgaaaaa gatctcttac ggcaatctat tttatttttt tgatttttta
   192301 taaatggaaa atgcttcaag aagtccaaga aataaagccg tataaaaaca agaacctatg
   192361 aaatctttat ttattagata ttcaacagaa atcccgataa cactagccaa caatatattt
   192421 atcagaagtg taaaataagg atgcttttta tagaatagaa ctagtgttgt tttcatagac
   192481 tctcctttct tcatatttcc aattaatcac aaattcacgg cattactaga aaaattaaag
   192541 gggcgcagaa gtggaaaaat actcttcgac tttccctaca aaccaggcca tcttgtgttt
   192601 aataactata ccaaatctaa ggtacctcta aatctaaatt ccatttgtgg cggtgacctc
   192661 caatttttaa ctcttctatc tcccatccag tatacataac cttgatactt cgaccactta
   192721 tatatcgaac acacaaatac ctatccattg caggagtact acctaactta gttgctactt
   192781 gcatctaaat aacagctgaa aagataggac ttactcttag taaagtatct gaactattaa
   192841 tttttaaact atcattttta tcctcaacta catgcacatc tgttgcaact tcactttctg
   192901 tctcatccag ataaatgatt aaggaatcgg gaattgcaat atcttcagct gacgctggaa
   192961 tagttgagct aaattagtca tcatatcttt attataaaat aaccaacagc aaaaaataca
   193021 atgaaaaaca aacttggaac taatgtatca ttaataaatg ttgaaaaatt cagcaactca
   193081 ttactgtatg attttagcag cccaaccaac atatacccta taaaagctaa taagatggtt
   193141 aggaatcctt tagaattttt actcattcat agacacttcc ttaaataact tttattatag
   193201 aaggtattgt aaataataat attgacaaaa agtgactcaa aataaaaaaa accttacatt
   193261 atactggaat gaactgtatt gatagtgtac ctaaaagctc tactgacata agatcgtgta
   193321 atcggtaaag taattttatt aattatacgg gatgtaatca attagagaat aaactaatca
   193381 taaaaggtac ttttacattc ataattgtcg ctttgagatt ctactttggt tcactatctg
   193441 acccaattgc acattttcga tcattacaag tattggtgtc gttagaacaa ctaaataaaa
   193501 actagcggta gatttttcag aatctatgct aatttctgta tggatgttcc agtgatggca
   193561 aatttctctt ttcattgttt ttcttgtcgc atgatatgcc tttttgtgtt attcagttaa
   193621 actatagaaa ttctttccta gacagatctt gatcccaaat tttacgtttt tgagcatcga
   193681 tatagtttaa aatgacatta ctcatatcta aagagtttag attttttagg actaaaactg
   193741 tcagttgttc tttttcttct ttctgttaaa gtattgtcgc gacttttcgt atacgttaag
   193801 atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg aatgacttga ggaaatcacc
   193861 tcaatcattc gtttaaaatg ctatatattc tttagtttaa gatacggaaa ttaatgccat
   193921 cgaccacact agtagaatga acgacattta cttggccttt ttttaatttc gttatgttta
   193981 gcatataaaa atattataga gtttacttac aatgcttaaa acatatactg tataatctga
   194041 tcaattttag ccatgtcttg caatggtaag ttttctattt ttttgagttt tcgttcctta
   194101 aaatcaagag attttaattg tgtaataatt acttgtcctt ttatatctag ctcttcaggt
   194161 aatgagtaac gtgtcggtaa attctttata gtggaagtaa ttggacatat gaccgaaaac
   194221 attgttttac ggttaaattc ataacgagag acgactagtc ctggtcgtct tttttgaatt
   194281 tcttttccta cggagggatc aaaatcaatc catacaatat cacctttttt aggaatataa
   194341 attctttctt ctgacactat aaaatttctc tcccttcagg agttaaatct tcccattcgt
   194401 ctttctcata atactcggcc tcttccccac cactaaatgg atcttcaatt tttggaacta
   194461 acgtaattgt tccatcatca gaatacacaa cgatatattc ttggttttct gaaggtttct
   194521 ttccgttatc agaaggtaaa gttaccacta cagaacttcc ttgtaatcta gattttgtaa
   194581 caagcattat catcactccc ttaataaaag tatactatat aacactaagt aacgcaaaat
   194641 tcccctttgt aaactatcta gaaaattgtt ttgtttaaag tatatcttat tggatataaa
   194701 tagaaataaa ttgtagccag ttcatttttt ctttaactaa aaaaataatc caagaaacat
   194761 tgaacctatg gatccaaaag ctacccaaat actatagcta accccccgct accttcaatt
   194821 ttgcagataa aacgctttgg gagaaaattt caaagacaac aggccatcct atcatgcccg
   194881 attataaaca aaaagctggc tattcctttt gctctgacaa atttcaattg ggaaagttca
   194941 cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa agaatgcccg cttcaaatag
   195001 aaacattggt tcaacgaatt tcaactagac aagactttgc tattgtagaa cgtgagatcg
   195061 tagctatttt tgtagaagaa gggattttac atgatccaac tcatatcaat gtggataaat
   195121 ggcaacctct tatctataaa ttcagagaat atacaactgc caaccagtcg ttaactttga
   195181 attttagatt ccaagaattc aaagagtcat aatgataaac atcgttaggt ataaataaaa
   195241 aagactcgca gcacgagtaa aaaacaaata gaagctatac attagaaagg tcgactgtga
   195301 ccctatagat gcagtatatg cagcttatga gcatttcttc tgttctcttt gtttttggga
   195361 tctgtgctga agtctttttc gttttttgcc gctctttctt tgaaaaatcg attatgttat
   195421 accttctcca ttactaattc aggcatctgc tgagtaatga tttttcgcgc aaaagaaata
   195481 tctgttaccc aaggttcgat ttgttcttca gtcaagacaa gcggcattcg gtcatggatt
   195541 ggtgcgactg tgcggttagg ttcagtagtc attatgattg attctgccat tttttcatcc
   195601 tgatggctac gataaaaacc gccaatatac actatttttt ccttatttga aaacataaat
   195661 ttgtcttttt cttcattcca ctcattggga aaacacaacg attttgcaaa aatggttgat
   195721 gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat tgtccttttt
   195781 taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct tcctcttttc
   195841 ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct gctgcctgct
   195901 tcggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac tctagatcaa
   195961 aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat acactgcaac
   196021 aagaagttcg caccaacaaa acgctttcaa tttttgtaga actaaaaaag ttgtgacaag
   196081 tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa gtcacaatat
   196141 tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag tgaaacggca
   196201 ggcttacgac ctctgggtaa acggtgttca aaagccagtt tctcggtatt aagccaaatt
   196261 tatgcaaaaa tgtagaaagc tattttcaca aaatttttct taatactcga aactaagcga
   196321 ctttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagccc
   196381 aaccaaacga aaaatatgag cagctatttt tatttggctg ttcttattac ttgatgcagg
   196441 gcagcttttt cacaacctct ccactaaacg gtgttcaatc agctaacctt cggtattagc
   196501 tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg tttctctaat actcaggtca
   196561 aagcgctgat ttcacaacct ctaatacacg gtgttccaga agtagcttct tcgaaaaata
   196621 agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   196681 gctgaccact tctgtcacaa cctctaatta ttaaagaacc ttttgcgaag ttaacaatct
   196741 ttctttgatc aattgaaaat tctgcctaag tagtgataga tcctgtgtga ctttcaacaa
   196801 taagatactt ccctggattt cagcaatcaa cgctttggct tgacggtctg cttcttcatc
   196861 ggacagtttt tcttcagaaa gtgccaggat attttgtttc gtatacttga cccattgatt
   196921 gactaattct agtagcaaca gacggtattc ttcatccact gtactcaact caataatcaa
   196981 attacctaca ggacagccat gataagtgat ctgtgtctca tgatcagcga aaatccagtc
   197041 caacatttct tctaatgctt cttctggctg ttttccagca tttaagatgt cttcgaacaa
   197101 ttctgtctgc catacgtcca tatgcgcatg gatcaccgct agacaaactt ctttttttga
   197161 agaaaagtaa taataaaatt gaccttttcc tgtatcagaa gctgctagaa tatctcggat
   197221 tgacgtttca tgatagcctt ttttataaat aagatctttg gtcgcttcca agatcttttc
   197281 ctttgttttc aatgatattc tcctttttca tttctcatag tcctatatta tacagtaaat
   197341 tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc caaattatct gacacagccc
   197401 tcactataaa actgttctca tatagctaga tgttcattga ttcaagaact gcgaggacaa
   197461 aagcaattcc tatcttatga tagttgctct aatacttcct ctgttgtcat cacttgatct
   197521 gcaataaagc caaagttcgt caacgctgca tccattgctt cttggccagg agcaccaatt
   197581 gcatctgtcg ctaccacaac attgaatcca ctctctacca attcacgcat atgggaatcg
   197641 ctacacaagt ttccgttcat tccaccaaga attactgtgt cgattccatt ttttctcaat
   197701 tggagcgcca aatcattgct ttctggtcca aagattttat gaggagagct gatgatcgta
   197761 ttttcatcca aataaggttt caattcttcg ataaaatcgg ctcctgaacc ttcttccact
   197821 gattcatatt gactttttct agcaaacatc tgctgctcaa gcatcatatg ttctcctgca
   197881 ccgccaaatt gccatttatg gtcatgtggg taaaaataat gaggagaaat aaagagtttg
   197941 tatccttttt cttttgcttg ttttaccatt tcaatcatat tttcgatcgt attgtatttt
   198001 tctaagatat cttttgtcaa accaaagcca ccgcctgctg gtttcaagaa ctcgacttgc
   198061 gggtcagtga gaactactgc tgtattttta ttattgaaca tggtggattc tccattcgtt
   198121 atttatttta atacatcatt tagagcttca ctcattgtcg gatgagaata gatttgatct
   198181 ctcaataacg tataaggaag ttttccattc atagctagcg aaatcaagtt gatgacttca
   198241 tgagaatctt ccgcatagat acttgcccct agaatcaagt cagtttcagg gtcgactaaa
   198301 actttgaata cacctttagg atcttctaat acttgtgctt tagggacacc gcttgtcata
   198361 tacttgaata atttgtaatt gacgccttgt gctttggctt gtttttctgt taaacctaca
   198421 ttggataaag tcggagtgat aaatacactg taaggcacag ctttccggtt attcgttgtt
   198481 cgtttattct cgcccttcaa ctgatccata atgattcgat aatcatctag agagatataa
   198541 gtgaactgca atccgccttt gacgtctcca atcgcccaaa tgttgtctgc atctgtacga
   198601 aggtattcat ccacagcgat tgctccgcga tctgttagtt taacatctgt attttctaat
   198661 ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca aaacttttga cgcttggatt
   198721 gttacttctt ggccatcttc tgtaaaggtg acggcagctg ttgattcttg gtctgctact
   198781 tgatctacag atacacctag atggaattcg attcccgcat ttgtcatgtc ttctaaaatc
   198841 atttgcgcga tatcttcatc ttcacgtgga agaaattcag aatgggcatc aagaacgaca
   198901 actttagatc cgtattcatt gaacattgat gcaaattcca agccaatata tccagccccg
   198961 atgatcacta atttttccgg taattgttcc tgatccatcg cttgcgtaga atcaatcaag
   199021 tagcgacttg tattcaatcc tttgattggt aaaatgattg gttcagaacc agtattgatg
   199081 aaaatccgtt cgccttcaac aatcactttt tcaccattat tcgtcacttc gatttgatgg
   199141 ttagaaagaa aacgtgctgt accgtccatc actgtcccag ttgcctcatc gctgatcata
   199201 tgataatttt tggcgcgaag catgcccgtc aatttttctt tccgagcaac agcctctgta
   199261 aagtcgattc ctctttctcc attgaaaatc aatgatttag aaggaataca gccgatgttg
   199321 atacatgttc cgccatacat acgtgttgac ttttcaacga ctaaaaccga ttcacctttt
   199381 gaagctaacg ccttcgctaa agtcttaccg ccctttccaa aaccgatcac aatattttga
   199441 tatttttcca tcatttttcc ctcgcttctt ttttacctac cgattggtac aaaaatatga
   199501 taaccgataa ttgtctattc tccaaatcat ctgctttatt tgtcaaaaaa aaccagacaa
   199561 aaagcggtcc tttcacgttt ttagtctggt tcgatttcct ttattcttct aaacaatcgt
   199621 tacttctaca tcgatatttc ctctagttgc tttggagtaa gggcagaatt catgcgcatc
   199681 ttctgcaatt ttttgggctt cttcttcgct gatgccatcg atatgcactt ctaatactac
   199741 accgatctga tagccgccat catctaaatt ataaagagag acacgtgctt ttactgtgga
   199801 ctctgctgtc attcctgctt ctttgatcat atgttgtacc gccccattga aacaagaagc
   199861 ataacctgct gcaaataatt gttctggatt tgttccttct gcatggattc ctggaggagt
   199921 gattttcata tgcatcgagc catttggtgc ctcgacttct ccattgcgcc cgccatggtt
   199981 gatcattgct gtttcgtatg cttttttcat tgtactgctc ctttcgattg accatttgct
   200041 acttggctat aattatagtg tatgacaagt aaggcgcttt ctacaaaaat tctgcttatt
   200101 cgaataccag aatgccgtct tctactattt ttccaaaaaa caaaaaaata ctactcgtga
   200161 acaacagcgt catatttgtt ccaaaataaa tagcaatctt ttctacataa tcttttatat
   200221 gattgatttt aagatacata tataccagaa agttgacccc ataaaccaat actgttaaaa
   200281 aaataaccat attcgttacc attttttcct ccttttgctg gtctccctac cagcactcga
   200341 tttaaccata ccactttttt tgccgtccaa gcaactagcc tttcaaaaag actggtttat
   200401 tctgctgtta atgattcaag atcagaaaaa tgtccgaata attttgctaa ttttactaac
   200461 gccaacttgt tctttccgaa ttcatcattt tctatctgta atactagtaa cggctcatgt
   200521 aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct cttctagatc atatcgaata
   200581 ccttcttcat tttccgggtc gaagttcatc cctttttctt cttccaacag tcgcttcatt
   200641 tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc tgaacaattt taatcggatt
   200701 tcttgtactt ctacgggctg tttcaactct ttgatttgtt catctaatgt gatacctttt
   200761 ctttttaatt ctggcaaaag catcaggatc tttgcgatga catatgcacc atcatccaga
   200821 aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga tggctagctg tgtatctttc
   200881 ccaagacggt taagttctaa tgctttattg attacatttc gatagccgga aatataacga
   200941 acttggatgc ctcctttttc ttcaatgaat ctttgtagat gagaagaagt aggggaattc
   201001 gtcacgattc ttgttcctgg atgctctttt aatacgattg tacttaatac tgcgatcaaa
   201061 ttatttcgat tgatcactgt tccagaagca ctgacaactg cagaacgatc cacatctgta
   201121 tcgaaaatga tcccaagatc tgcttcattt gctaaaacag cctgcttgat actttccatc
   201181 gcttctttat tatcaggatt tgggatatga ttaggaaatc ggccatctgg ttccaaaaat
   201241 tgggaacctg ttgaatccgc acctaagcta ttcaatactt tttcagcaaa gaatccacca
   201301 gcaccatttc cggcatcaac aatgattttc caaccattta gtggtttgct ttctagtgtt
   201361 ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat aaggtgtcaa aaggtctgct
   201421 ttttctacgc ttccagtttg ttcgcttttt attttttgct gctgtgatag gataaaagca
   201481 atatcttctt tttctgctcc tccttctgag gtaaagaatt ttattccatt ataataatag
   201541 ggaagatggc tagcagtcag cataaccgca gcatcgcacg agaactgttc aaattgagtg
   201601 gacataaaca tagcaggcgt agtagctaat tcgaaatcga tgacatgtat cccttgttct
   201661 ttcagacttt ggatcaacgc ctctttcagt gctggtccgc ttatcctact gtcatgacca
   201721 atcccaatcg tccatttttt atttggatcg gttgatttct tctttttctt tatccagttt
   201781 gctagtcccc agccgatctt ttttaccatg tctgtcgtca agtttacttc atatccttca
   201841 actggtaatg cgattcctcg aatatccgat ccattttgca gcgctttcat atctttcata
   201901 taaaagtcct cctcatttat tccctcattt tttccagctt ttctgcaaat cgctgtgctt
   201961 cttcttctga gcggcaaata ataatgaatg tatctacacc ggccatcgtt ccgacaactt
   202021 cagggtaagc cacttcatct agccaattcg ttaccacatc ggcaccattt cgttcagtca
   202081 atacgataac catgaactga acagactgga tacgcaaaac ctcgcgtcta attgctgtac
   202141 gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa taatgtatag cgtactattt
   202201 tgttttcatt ttgacttttt actaacttca tttctcgaat gtctcgagaa atcgttgctt
   202261 gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag ctcttcctgt gtcccaatag
   202321 gttgttctgt aatgatctgg cggatcagtg cttgtcgctg tttctttttc atattagaca
   202381 cctctcaatg ttgattatag caaaagataa gtaatatttc ttcatatttt ggtatttttt
   202441 acttttattt tttgttttca ttcaggttgc taaccttttt ctattttaac acgcaaaatc
   202501 gtcttcattt tttcaggtgc tgattttctt ggatcgctga tgtaaatctc tttgtgttct
   202561 ttagaagctc tttcatagcc ttcttttgcg atgaatgctt ctaatttctc aaacgttgct
   202621 ggttcttcgg catagggccc gacgtgtaag atttgggcac aaagaccttc ttccatcttt
   202681 ttgaatacta cttgttcttt caagtcttcc ggaagtttgc tagaacgctg aatggcttct
   202741 tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca tcaattgata agaaaaatgt
   202801 tcttttttca tcactggttc atgcaaatac ttttcttgca gtccccattg tccttccagt
   202861 ggatagacag tatacatctg ataatcagga atcagccaat tttttttagg cgacatgcgt
   202921 attgcatagc tgactgcata tagacaggct actcttcgtt taaagtcttc ctgattcggg
   202981 tcgccgatcc ctctgatgct aaagaatgat tttttaggaa cctctagtat ttgcgggctt
   203041 tttttcatac tatagctctt gtctttacgc cattcaattt tttccatttt tcttcctccg
   203101 cgaaattcca tttagttatt tattatcata cctctttttg agtgacttgg ctaatcgtgt
   203161 tgacactatt tttcagtttg tgctagaata agtgaagtaa ataaatcgta cagataacac
   203221 gtttcggcgt gtcgagagag aggcggtttc tatttttata atagaaatca ctctcttttt
   203281 ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa aacagctgct tttagtacct
   203341 gcagtaattg caacagggat gatgtctttt gcgggcgtat tgatcgagac ggccatgaat
   203401 gtaactttcc caactcttat cagacagttt gatttaacga ctgcacaagt ccaatgggta
   203461 acaacgattt atcttttgat gatttcgatc attgttccgc tgtctactta tttgaatcgc
   203521 aattttagtc tcaaaaaact ctttttagct tcaaatctgc ttttcttggc aggtataatg
   203581 atcgatttct tctctcctac cttcatggtc ctattgtttg gtcgattgct ccaaggtgca
   203641 gcgacaggta tcgctcttcc gttgatgttc catattatct tgacatttgc ccccctcaat
   203701 cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg ccattgctcc agcaattggc
   203761 cccacttatg gaggcattat gacttcacat tttacatgga atcatatctt cctgtttctt
   203821 gtgcccgttt tgtttctttc tttgattatc ggctggtttg ctattcccga gatggcagta
   203881 caaaaaagtg gttatttgga ttggataagc gttttaggaa tttgtctttt gtttagtgga
   203941 agtctaacat ttttgagtac gttgggaaca gtttccagct ggattgcttt aatcatcggc
   204001 ggtattggct ggttcctttt ttactatcga agtaaaaaag tggagcatcc actgatccga
   204061 ctgtctattc tttcaaataa aacattccgc ttgtttttga ttggcttctt agtttatcag
   204121 tttttattac ttggtgtatc ttttgtcttg ccgaatttcc ttcagatcgt tcaaggaagt
   204181 agtccttttg tcgctggatt agcgatgctt cccggcgcag cggttggtgc tttgctttct
   204241 cctttttccg ggaaaatgct ggatcaatat ggtccgaaga agccaatttt tgctgggctg
   204301 attctttctt tgattggttg gctagcatta ttcctactca taggacacgc ttcagtgggt
   204361 gtattgattg cttgtcatgt gttttatatg atcggtatcg gattatctta cagcaatatg
   204421 atgaccaccg gaatgaatgc actgaatgaa gaactacaag gagacgggaa cgcaatgttc
   204481 aataccttgc agcagttttc tggcgctgtg gctacttcat tagtagcggt cattatcaat
   204541 tacgtccagc atcatacttc ccataactat gaagtgtcta ctacattagg atcaaaagca
   204601 gcattaggcg tcttgttgct gttgcttctc gtaagctttg ctcgattcgc ctattatctg
   204661 ttctttgcta aaaaagcgta gattccatat acggcaggtg ttgcatctgc cgtttttttg
   204721 tgtgaaaatg gttgtttgta aaaggaggct tttttatgga aaaaattgcc attatcggtg
   204781 gcgggatcat tggtatgacc ctagccagtt acttagatac atcaaaattc aatgttgctt
   204841 tatttgatga agggacagga caggcaacta aagctagcgc tgggattatc tccccttggt
   204901 tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa agacggcgct gcttttttcc
   204961 ctaaacttat aaaagatttc caacttgatc aaacgattta cgcacaatca gggacacttt
   205021 tgcttcgacc tgaaaaagat ttagctgaat tggctgttct tgccgagaaa cgaaaagaag
   205081 aagcgccaga aattggaaac atcgaatggc tttcacctga acagacatct caagcacttc
   205141 cgttattgaa accagcagtt tcactgcgta tcagtggtgg tggtcgtctg gatggaaaag
   205201 catatttaga acatcttggt cagcgtttag cacaaaaaca aatggagcag atccaggcaa
   205261 aagtcacttt gcataagaag agcgattctt ggctcatcaa gggaccgttt ggagaataca
   205321 cagctgatcg tgtaattttg acacctggcc cggcacttaa gcctttatta caagaattag
   205381 gctatgctgc agatatacgt ccgcaaaaag ggcagctctt agcatttcag acatcttata
   205441 agatgagtca agactggcct gttgctatgc ttgacggaga agctgatttc attcctttca
   205501 tggatggaaa gatcctttta ggtgctaccc atgaaaacga tcaaggctgg gatttggctc
   205561 ctactagcga agcttataag ggattgacta agagtacaga acagttcttg actgatccag
   205621 atcagataaa aaactggcca tacgagttcc gtgtaggtac ccgtgcttat acgtcagatt
   205681 tttccccatt tttcggcgta ctgccagatg atccaagctt actggctgct agcggtctag
   205741 gctcttctgg cttgaccaca ggtccttata tcggctatct attagccaac ttcttaaata
   205801 ctggtcattg gcctacggaa gattatcaaa aggacatctc cacctatatt acgaaaaatc
   205861 catcatagaa aaacagcttt ttcaagtaag gatcccgagc gatattgcgc atggattact
   205921 tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa attctccaaa ctttcttttg
   205981 cttcatctgg caagagatct tttcctatct gagcttcaat gaatcgtaaa cgaatatcaa
   206041 gtcgttgttt caccagattt ttataggacg tatcctcaaa agaaacagga aaatctggta
   206101 taggtaaata gtggatgtct tcaaatggac caaacgactt gaattctgcc gtttgttgca
   206161 cgattctttc aacgattgac aatgtcgttt caggtttgat ttttttatca tcgaaatatc
   206221 cagtattcag gacatagcag gctgtttgct gtttcaaaaa caactctcga aaagcttgat
   206281 agtcttccct aagaggatag acacggaaag gattagcaaa tggttcaatg actagctcat
   206341 ctagctgtct ttttgtagtg atattttccg cgtctgatcg ttttgttgct aatgttgcac
   206401 caaaagacgc tgctaaaact gggtcattga tcttgataat tggcggaagc gaatcatctt
   206461 tcatgatcca aaaaacagca tcgatctttt cgctaagatg atcgactcgg tcgggtgtcg
   206521 cataacgtga tttgactgtc cgcccatttc cgtttcgtat atctcctgtt actaggactt
   206581 tttctccttt acttgtacat gtcacgccca cattctgaca agtaaggaaa gaagaaatgg
   206641 ccggatcatc agacggatag tcttgtgttt tatcaaagta agaaggttcc aaggcagtag
   206701 caaaaccatt ttttcgagaa ataacgaaag catcgtcatg caagatcgaa acagagtgac
   206761 cttccgtatg ttttgctaag gtaatcgttg attttccaga accggacaac ccaaatacag
   206821 ccatcgtata ggtctggtca ttactagcat attgtttcat cccaccgtgg caggcaataa
   206881 agccattgcg atttcccaat gcccaagcaa gcgtcaaagt cgcttttttt aattctccaa
   206941 agtaacgtaa tcctagcaca gccgctgtat tatggagagc atcaaacaaa atgagtccat
   207001 ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat ataaagatct ccctcatcaa
   207061 aggaaacaga cttttgataa gtctctaaga tcttcgaatt cgctggctga aaattcagca
   207121 aataagaata caaattgttc tcaaatcctt ctggaatcag caaatgactt tgcacagaaa
   207181 attcttcaga taggccaacg attacttttg tatggtaaaa ctggcgcttg cgactttgat
   207241 acacagcttc tcttaaaatt ttctcgtatt tgctttgttc cttgtctaag tcctcaaaga
   207301 tccgccgagc acttgctgtt cttcccacaa tgcttccatc attgaatact agtgtcctaa
   207361 catcagctgg taaatctagt tcttcagcat gtgagattgt taaatctgtg atgatcgttc
   207421 ctggactccc ttttgctagt tgataagctg tcttcctatc aaaaacttca tgaacattat
   207481 taccgtaaaa tgtcgtttct acaatcgatc gaatactgga taacagtgga tttccacttg
   207541 tgatttcgtt taattcgaga ctctcttttg tactcatggc tcctcctcat tccctaaaca
   207601 aaaaaacgtt ttctttatta tacattattc gagtttttat ttgtactgct gcgctgtggg
   207661 aagcaaacga gttatttttc acacaaaaaa accgagaact tgaataaaca agtttctcgg
   207721 tttcatcatt ttccttttat cggcttagta ccagccgttt gctaaccaga atgttttagc
   207781 agcatcccat gaaccataac gagatgttac atattgttct gctacacgtt cttgattggc
   207841 agctgaatag tcgccgttta agtatgaagc atctaattga taacggccga tgtaacgtcc
   207901 gttagtagct gtgtatgaac cgcctgattc tttttgtgcg atccattctt ttgcagagct
   207961 tgaagttggc gctgttgcag cagctggggc agcaggagct acttcaactg tttcaacagg
   208021 tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta gatgctgtat tttctgttgt
   208081 tttgttttct gttgttgctg aagaatcatt tgaagttggg attgttaatt gttcaccaac
   208141 ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca attgcatcga tcattgagtt
   208201 gtctcctgca aatttttgtg agatcttaga taatgaatca cctgatttta ctgtataaac
   208261 ttcatcagcg tgagcatttg tagttcctac aaacattgca gtagcaccag cagcaaaagt
   208321 tgtacctaaa acaattttct taaatgaatt catattaaac tccttttttt ctccgttttt
   208381 tattctttcg acgctattaa ctttatcatt tagaaatatt tcataggtaa aagaaacatg
   208441 acatttaatg acaaaataac gaattattta atatttgtaa catttaaaca aatatttttt
   208501 tgatacaaaa ccataaaagc cttgttgtat aagcaaagta atcgtttttc tatgtttttt
   208561 tatttttgaa tgaaaaacgc agattctgta acattcactg caataatcca taaaaaaaag
   208621 caaaaaaatt tttgattttt atcaaattct cttaaaaaaa tgtaaaaaat acaaaaaaac
   208681 cagtaattgt tacaacaatt actggacaaa aactttgaat aatgttaact atttttttat
   208741 tttaactttc tccgcaataa tttctgggca tgttccaatt cgtttataac gacttcttct
   208801 gtttctttag ctaatatttc atctaataca tcaagccatt tttccggttc ttttgttcct
   208861 agacgtccaa tagcccaagc tgctgttcct ctgatgacag ggcggacatc tgttaaacat
   208921 tctgaaatct caggcaatgc cgttcgatca cctagattag ctaaagcaat caatgcgttt
   208981 ctctgcaaag gtttcttccc acgccatgaa ccagctaaat gcccaaattg ctgtttgaag
   209041 tctttattag acatcgaaag cattggcttt aattttggat atatttcgtc tacttttggt
   209101 tccatctctt catgaaaatg aaaatctttt ccttgattgt aagggcagac taactggcag
   209161 atatcacatc cataaatgac atttcgcatt tttttacgat attcttccgg catcatccct
   209221 tttgtctgag tttgataaga caaacatcgc tgggcattca ttcttccgtc accaagcaat
   209281 gcttttgtcg gacaagcagt cacgcagcgc atacagtcac cgcaaccaaa ttctcctggt
   209341 ttatcaggct caaatacaat attagtaacg atctcaccaa gatagacaaa tgaaccgaac
   209401 tcttcagtga tcaacaatcc gtttcgtcca ataaatccta aaccagctcg ctgtgctaca
   209461 gcgacatcga tcagttctcc agtatctact tgcggagcta gacgccattc ctcttctttt
   209521 tgccattttt ctgctcttgt ttggatgaat tcgatcaatc ggttcaaacg gtctctgagt
   209581 atatcatggt aatcgatccc ccaagaagca cgagcaaact gcccgcgttt ttcatcccta
   209641 ggcacttctt catggatcct agtaggatac gcaagtgcaa tggaaatgat cgatttcggc
   209701 tgatcaaatg tcatttctgg ataaagacgc tcatcgatat ttggatgttc aaagccagaa
   209761 gtatggccag ctgctttttg ttcttccaac gattctttta atgaatcaaa aggttccgcc
   209821 gtggtgaacc cgattttgtc aattcctaat cgtttgcttt cttgtatgat ttcttctttt
   209881 aatgtcatgg tcttcctcgc ttgctgttaa cggctgtata acccataata tagtttttct
   209941 tttttcagct ggacaaactg gatctgctga tcggcaatct tcttttcttc catccatttt
   210001 tttcttgcag caaatacttc ccaataattt gttgaaatta attttctttt gttatctaat
   210061 gcattaaaaa aattcaatga aactggtttt gtacttaaca gttcatggag ccttacctct
   210121 tcccatggcg aaaactcaga cagatcgcgg gaatacaata cttcttcata gagatttttt
   210181 tgctgtgtgt acgtgaatgt ttgcgggatt ttcccaggcc tagcaatttc tactagacga
   210241 ttataaaaca tattcattaa tttgctgttg gcaataaatt gacttttata ataggccgac
   210301 tggatctttt tgattactcc ttctgctttg atgactgaaa ataatgcagg ccatgctttg
   210361 acggttattt cgtcgttttg tttaactaat acgccaggtg tgaagccgac tggagaaagg
   210421 aacacaaaat aaccgatacc ggtattttca tctaaaggct gaaattcatc aaacaaccat
   210481 ttgcctatgt acagctcttc atctggttct gttgtctcat tttgatttgc tatgaataat
   210541 ttagctaatt ctgcttcaag aaattgaagg tcttgatcct tgtcggtatt ttcctgctgg
   210601 ctgatcaatc gaaagacttc ttctttaatc gtatatttat ctttacgtgt aaaattcata
   210661 gccacacctt ccttccttac tctaactata acggaatttc ttcaaaaaag gtatctgtac
   210721 gacgaaaaac agcataattt tttttcatat ctcttataaa aaatgcaagg tctgctcctc
   210781 aagctagacc ttgcactttt cagctttctt ttgtttgtga caaaccttca tggatcgttt
   210841 caatattcca tttcatcatc tgatagtaag aatctccttg ttctccttct tttgctatgg
   210901 aatcagtgaa cagtttatca tagattttca aacccgtttc ttttgctacc cgttccatgc
   210961 ttcttgagtc tacactggtt tccacgaata aaaccggtac ttcttttgct cttatctgat
   211021 caatgatcgc tttcatttga tcaggcgtac cttgactttc tgtattgatc tcccatatat
   211081 aagctgctgg caaatcatat gctcgtgaaa aatacttgaa tgccccttca cttgttacta
   211141 ataatttttt gttctcttcg atagaagcaa aactttcctt cgcttgtgta tctaattctt
   211201 ttagtttttc cacatatgct tctgcatttt ttttatagag tgtcgcattt tctgcatctt
   211261 tttcagagaa tatacgtgcg atttcctcca catattttat tccgttagat aggtctagcc
   211321 aagcatgggg atctgctttt gtatgttctt caccgctagt taaataaaga ggttctacat
   211381 ttttgctaac tgcaaaataa tcttttcctt cttctttttt agccgtttcc atcaagttat
   211441 cgaaccagct gttacctgtt tcaagattca aaccgttgta taaaataaca tctgcatcgc
   211501 ttgccttttt gatgtcttct ggtagtactt catattcatg cggatctgtt ccgactggta
   211561 cgatactgtg gatatctatt tgatctgttc cgacctcttt tgccatgtcc gccaagatcg
   211621 aattggtagc cacgactgac aacttttctt tacttccttc atccgcttgg tcagaagcct
   211681 gctttccgca accggctaat accaagaaac taactgccaa gattaaaaag agtgattttt
   211741 ttgttttcat ctatctgctt cctttctgaa ataaaattcc ttttgttgga gaaaataaaa
   211801 aagcaaggat aaaaaatgcg gtacatgcta acacgatcgt agctcctgat ggccaattgt
   211861 aactataact gaaaaacaca ccgacaactg cacttagcat accgattcct gcagctgtaa
   211921 tgatcatttt cagtaaatgg ttcgttagca agtaagccgt ggcggctggt gtgatcaaca
   211981 tggcaatcac caagattgtt cctactgtct gtaaactgac tacagccact aatgtcaaaa
   212041 agaacatcaa aagataatgg atcagcgatg tgttcagccc ataagctttt gccattgtcg
   212101 gatcgaatga tgtgatcttc aattctttat aaaagaaaaa gacaaatatc aagacgattc
   212161 cactaacgag tgctgtcagc aacaaatcgc tttccctaac agctaagaca ttcccaaata
   212221 aaatatgata caaatctgtc gcactctggg catatgaaat caagataatc cctaatgcaa
   212281 aaaaggagct aaagacaatt ccaatcgctg tatcattttt caatcggctc cgctgagtca
   212341 cgaaaccaat cactgctgct gttagcatcc caaaagcagt ggctccaaag atgtaactaa
   212401 aaccaaacat ataagaaatg gctacacctg gaagaactgc gtgagagata gcgtctccca
   212461 tcaaagacat ccctcgtaaa ataataaaag aaccgatgac tccagaagca agtcctacaa
   212521 gcatggaagt gaccaaagcg tattgcagga attgataatt cattaatcca tcaataaagg
   212581 attggatcat tttttctctc ctccttttac taaatgtctg atgatctcac cataggccaa
   212641 ttgaaggttc ttcgatgtaa agacttcttg tactggaccg gaagcgatca gctgtttatt
   212701 taaaagaata acctcatcga aatattcttc tactttatga agatcatgat ggacgatcaa
   212761 aatcgttttt cctgaattct tcaattcctg caagatgtca aagatctttc gttcacttaa
   212821 cgcatcaatc cctacgaatg gttcatctaa aaagatccat tctgctcctt gggctagagc
   212881 tcttgcaata aaaactctct gaagctgtcc acccgatagt tcgctgatct gtcttttggc
   212941 atattcttcc atccctactt ttttcaaagc ttgtcttgca cgttcttttt cttttttccc
   213001 aggtctttgt ccaattcgta aagatggata tgttcctaaa agtacaacgc ctaatacatc
   213061 aattggaaag gaaagatcca attcacttcg ctgttccacg taggctattt ttttacggac
   213121 tgagtttacc gactgtccag aaaaggtgac tttaccagta gtagctggta tcaactctag
   213181 caaagatttc ataaatgttg attttcctgc accgtttggt ccgataatac cggtaatttt
   213241 ttgtggattt atcgtcaagt tgatagtttt caaaacagtt ttcccttgat aagcggctga
   213301 aaggtcttga attacaatgg ccgtacgttc caaatcttac actccttttt aggtttacct
   213361 aactttttat ttaaatatag tttaactaaa atggaaagca ttttcaagta gaatgatgaa
   213421 aaagtttttt tcgaattttc ctcgcatttt atgaaaattt tctgtagaat cacatcaaga
   213481 gttcaataag gctaacctaa taatgaaaaa catgtgttga aacgtttgat tattttttct
   213541 tgttgactca acaacgaaag gagtttttgt atggaagaag aaaagttaac tcgaagtctt
   213601 agtgcccgtc atatccaaat gatcgcacta ggtggaacga tcggtgtcgg tctgtttatg
   213661 ggtgcctcct caaccatacg ttggacaggg ccctcagtga tgggagccta tgcgattgca
   213721 ggattctttt tgtatttgat tatgcgtgct ttaggcgaaa tgctttacgt tgatccttcg
   213781 actggttctt ttgcaaatta cgcgagcgag tacatccatc ctgtggctgg ctacttgacc
   213841 gcttggagta atatttttca atatattgtc gtaggaataa gtgaagtgat tgctgtagga
   213901 tcttatatga attattggtg gcctgatttg ccggcaatta ttcccggaat cattgtcgtc
   213961 ttgtttctta tgctggcaaa tcttatttct gtcaaggcgt ttggcgaatt agaattttgg
   214021 ttttcgatca tcaaagtcat aacgatcatt ttgatgatca ttgcaggtct tggggtaatc
   214081 ttatttggat ttggtaatca tggtcaggca gtagggattt caaatctttg gaaaaatggt
   214141 ggattcttca ctggaggcgt caaaggattt ttctttgcat tatcgatcgt tgttgcttcc
   214201 tatcaaggaa tcgaactgat cggaatgact gctggtgaag cagagaatcc taaaaagacg
   214261 atcattgaag ctgtccagtc cactatcggt cgaatcttga ttttttatat tggtgctatt
   214321 tttatcattg tgagtatcta tccgtggaat caattgagtg agattggttc accttttgta
   214381 caaacttttt ctaaagtcgg cattacattt gctgcaggac tgattaattt tgtcgtgatt
   214441 acagcggcat tatcaggttg caattcaggg attttcagcg caagtcgtat ggtctataca
   214501 cttgccataa atggaaagat gtctaaaaaa tttctgaaac tatctcgtca cggtgcgcct
   214561 ttttatcctg tcgtagcaat ctcaggtgga atcttaattg gtctgatctt aaattatctt
   214621 ctaccaatgt tcatcaacca gtcagaaaat attttcgttt tcgtatacag ctcaagtatt
   214681 ttgcctggta tggttccttg ggtagtgatt ttgatcagcg agattcgctt caaaaagaag
   214741 cacgcagata aaatgcatga tcatcctttt aaaatgccac tatctcctta tacgaattat
   214801 gtgacacttg cttttttagc tatcgtattg attttcatgt ttatcaatcc tgaaacgaga
   214861 atctcattga ttattggact cgttttcttg ctttacatga cgatccatta ctttgtacgc
   214921 gaaaaaagaa ccggaaaata aaaaaagaac gaagcttcac cttagtggaa gcttcgttct
   214981 tttttattgc ttttatcaat tcaattttag cgtctcaccg ggagtcagga aataattctc
   215041 catgctcatc ccattcagcg acaagatcgt ttctactggt acgccagttc gttcggctat
   215101 ttgatttgga ccttctcctt ccaaaactac ggttgtcgca gcctgtgaag tcgcgctgtt
   215161 ggtattagtt tcagaggaat ctgttgtgtc ttgtgttgtt cgagcttgca tttcagaagc
   215221 ttctaccgac tctgctgtct cagaatcaga agatgttgtg tcttgtgtgc ttgttgactc
   215281 tgcttgaaga gaggcgctag tctgcaaata aatatcggaa atcttagcga catgattcgt
   215341 ataattagcc gataattgat tgatcaaagt atctaagtct acaccgtctt tattttctgc
   215401 tgcctgatac gctgcacgta attttctgat cccttcgtcg gagattgctt gaagctcacg
   215461 tgctttttgg ttagcaactg ctgataaacc agctattccc tcttgatttc cctgaatttc
   215521 tgtctggtat tcttcaatca atgcaggcgt cttttcttcg atctttttcg aataatcatt
   215581 tacgattgct tgaagttgct ctggcaaacc atttgctgtt ttttcttcag aagtttgtgt
   215641 ctcttttgtt ttagttgatg actcacttgt ttgtttcgtc tctgttttct ttgtcgttgt
   215701 ttccgttatg gtcgtacttt tcgcttctga cgaatttttt ccatgaaaat atagacccgc
   215761 aactcctcct gctatgatca aaaacacggc aactgctgcc ccaatcatga tcatccgctt
   215821 acgagctgcc tgtttttgct gtcttcttcg ggtcatccga acattttctc cccactcatt
   215881 ttcatcatac atttttttca ccaccaattt ttatgtataa tagcgttatc aaacgcttca
   215941 tattcttatt gtagcaaata attgtcgctg tgcacatttt tatctttgtt atttataaga
   216001 aaatttcacc agttgagatg attccaagat tgtccgtcga tttctccttg cagctgcatt
   216061 gttcgttcgt ctatctcagc catcagtttc tttatttcat ataagagcgc acggtcttga
   216121 tattctgatt tttcgcttaa ttcatctaat tgttggatca gccaatcatt tgtcataatg
   216181 aagttccttt catttcttgc agtgtaaagg tcaagtcttc gatctgctct tttgcctcag
   216241 tcagtgcttc tacgatatat gcttgttttt cagattcttc ttcctgcatt ttctgatttt
   216301 cctgtactct ttgaataaac cagtcaatcc gctgagtcag cgatggtttg ccttccaatg
   216361 agagatactg ctgataagca attagcttct cccaacgttt ttcctgggat aaatacataa
   216421 attgtgctaa aacgaatggc ggaagatagg tcatatggaa tttatttgcg attcgctgat
   216481 acggcctcaa aaattcggaa atcgtatatt cttcttttcc tccagcttgg tattcttttt
   216541 ccgccacacc tgttgtcaat acgattccta gttctttccc ttttaataca tgtgctgctc
   216601 gttccaagac attatcttgc caaattttta aatgagcagg agaactatac cagtaaaacg
   216661 gaaactggaa aatgatccga tcatgctggg ccaagagctg tagttcagtc tctatatcta
   216721 tttttccgtc tggataactt gattccaaat gatgccaagt agctggtaaa ccagcagaag
   216781 cttctttgaa aaattgctga tttaaagatt cttcgatcgt tggatggcta atgatgataa
   216841 gtgtacgcat cccttttcct cctttttagt cttcgcttta cagtgtagca taaccgccac
   216901 aagtttcttt tgccgatgct tgtcatttta accagactaa tcaggttgtc caagaaactg
   216961 cggtttgttt ttccaactgt tttttcaagg tatccaaagc aatattctta aaattttcgt
   217021 ttttcatacg tttacgatag ttcgtagccg tttctccggt actctttttg aacattcggc
   217081 taaatacgac tagactattg aaccctactt gataggcaat actttccacg gattcatccg
   217141 tcagagaaag aagaaacgcc cctcgattga tccgtacccc gatcaaatat tccttgatac
   217201 tggtacccat atgatttttg aatactcgcg acaaatggct ccgattgacc gctaaatgct
   217261 gggcgatttc ctcaacagac aatttttcag cataatgtgc ctcgatgtat ttgactgttt
   217321 caacagctaa agaggaatga accttttgta caccaccgct gacctttccg ccatctttta
   217381 aaaggactga taaaaaacgg taggttaatg ctgttaactg taattcattt tccaaggttt
   217441 cttccgtatc tttcatgcat tctaaaataa tgtcgatata tttttgtatg tcagaagaga
   217501 ccatcgtgta ttggtcttct ctgaaaagcg actgccgaat gatttcttct gcagcactcc
   217561 caccaaatga aagccagcaa tacatccacg gatcttctcc atctgataag taaaacgtcg
   217621 agtctcctgg acgaatcaag aacagatcac cttttttcaa ttggaattgt tgatttttca
   217681 catgataaga tcctttgcct tccatcacga catgcaggat gtaatcattg cgaatagcag
   217741 gaccaaaaga atgaaacggc atcgttttag agaaaccaca aaaatcaaaa tacaattctt
   217801 tttctacttg ttgatgttcc ttttttaaaa aaatcgattg ctccacgtct atccctcctt
   217861 actcctttag tataatgcaa caaaaagtta aataaaagtc acagaaatat attaaaaagg
   217921 aagcgatttc ttgttaagtt taagtcatta aaagatcttt gttattcgtt gtaaccggtt
   217981 tacttagtat tatatattga acaggagagg aatacagatt atgaaaagca aaatgatcca
   218041 acgtgtgtcc tacgctttag gcgcttttgg acacgacaca ttctatgtta cattaagcac
   218101 ttattttatg gtattcgtga caagccagat gttcgcagga acagataagg caacgaatgc
   218161 aaaaatgatt gctacagtta caagcttagt tgtaggtatc cgcttagttg aaattatttt
   218221 tgatcctttg attggtggca tcatcgataa tactcgtaca aaatacggta aattcaaacc
   218281 atggttagtt atcggcggcg ttgttagttc cgtactcttg gtagcaatct ttaccaattt
   218341 ctttggctta gctactcata atacgacttt atttatcata ttatttgcaa tcgttttcat
   218401 cttattggat agtttttatt catttaagga tatcgctttt tggtcaatga ttccagcttt
   218461 gagtacggaa acaaaggaac gtgaaaaatt tgctacgttt gctcgtttcg gctcttctct
   218521 aggtgcaaat ggtactactc tagtcgttgt ccctatcgtt actttcttta cctatcttgt
   218581 tactcataaa catattgaag gcgcttcagg ctggttctgg ttcggtgtga ttgctgctgt
   218641 tatttcaggc ggtactgcga ttatcactgc cttgggcaca aaagaagtcg atactgcgat
   218701 ccgtcagcaa aatgaaaaag ctgggatcaa agatgtgttc aaagcagttg ctcaaaatga
   218761 tcagttgatg tggcttgccc ttgcttatat cgcttatgcg attgccaatg tagcaacaac
   218821 tgccgtatta ttctacttct tcaaatatgt acttggccaa ccaactgaat tttggatcgt
   218881 tggtctagtt gctgccttac ttggtttagt agctgttcca ctattcccaa tgcttacacg
   218941 cgtcatctct cgtcgctacg tttaccttgg agggatcgca atgatgattt cttcttactt
   219001 tttctttacg atcgcaggtt caagtttgcc aatcgttatc atcgggctag tattgttcta
   219061 cttccctcaa caattgattt tcttatccgc tttgatgacg atcacagact ctgttgaata
   219121 tggtcaatgg aaaaatggta tccgtaacga agctgttact ctatcaattc gccctttact
   219181 agataaaatc gctggtgctt tgtctaacgg gatcgtaggg tttgtcgcta tcgcttctgg
   219241 gatgactggt aatgctactg ctgctgatat cactgctcat ggcgtaacga ctttcaaagc
   219301 ttatgcattc tatgctcctg ctgctttgat gattttctcc gctattattt ttgcttggaa
   219361 gattacattg acagagaaaa aacatgcaca aatcgttgag aaattggaaa acaaattggc
   219421 tccagctgat acaatggaag atgaacttca cgatgcagta gttccttccc attaagaaag
   219481 gatcttgaaa atgaagatta cagaaaaaga ttttggccaa ggttatcact tgataacctt
   219541 ggagaacaaa aacaagctag cactttctat ctctgattta ggtgctagaa tcgtcagttt
   219601 aaaatcaaat gatcgagaat tagttttggg atttgacacc gctgaagaat atatcgaaaa
   219661 agatccgtat atcggtgctt ctatcggtcg caccgcaggg agaatcgaaa atggccgctt
   219721 ttctctcaat ggaaaaactt accaattagc cacagatcca aaaactggac ataatctgca
   219781 tggtggtgct cctggatttg aattaaaaaa atggtcttat gtcattttga atggcgaaaa
   219841 tgaagcttct gtcattttta ctactacaag tcccgacgga gaacatggtt ttccaggtac
   219901 aatggatgtg gaaatccgct atactttgac aaaagataat atctggcgag tgacctctcg
   219961 cggcaccagt gaccaagata ctttattcaa tccgacaaat catgtttact tcaacttgac
   220021 gggagatgct tctcagtcaa tcgatcaaca tgagctttgg ctcaatagcg aagcttatgc
   220081 tcctttgcga acagatagta ttcctatagg ggtaaaagaa aatgctgcag gaagtgcttt
   220141 cgattttcaa atacctaaaa aattagcgtc cgtttttgct agtgatttgg atcaaaaaaa
   220201 tcttgttgac ggaatcgatc atccattttt cttaaaagaa accggcttgg gtaaagaagc
   220261 cgctcgcttg actagccccg ataaaagaat ccaagtagat attgctacag atgcttctag
   220321 tgtggttatt ttcactgcaa atttcggcac agaaacacca gaaatgcgca accgaaaact
   220381 ggcacatcac ggtggcatca catttgagac tcaaacagct ccaggtgcag agcgcttctc
   220441 agcatttggt tcgattcatt taaaagccgg atcagtattt gaaacagtaa cagaattcaa
   220501 aatcaaaaca agaaaggaat aactcatcat ggaaatcaaa agaagcttaa acacaaaatt
   220561 tgccgaaaca ttcggaccta aaaaaactac acaatacttc tcaccaggac gcatcaattt
   220621 gattggtgaa cacacagatt acaatggagg ccatgtcttc cccgcttcca tcacttatgg
   220681 aacgcaaggt gtagcagcac ctcgagacga taataaaata ttagtttatt ccacaaactt
   220741 tgacgatatc ggtgtcatta gttttacttt agatgagcta gaatatgata aaaaagcaaa
   220801 ctgggcaaat tacgtaaaag gaatggtcct taaattaaaa gaagctggac actcaatcga
   220861 tcatgggttt gagttattag tggaaggaac aatccctaat ggtgcaggac tttccagtag
   220921 cgcttcatta gaattattag taggtgttgt attagaagat ctgtttgatt taaaaacaga
   220981 tcgcttggaa ctagtccaaa caggtaaaaa agtagaaaat gaattcatcg gtgtcaattc
   221041 aggtatcatg gatcagtttg ccatcggctt cggtgagcta gatcatgcaa tcttattaga
   221101 tacaaatacg ctgaaatacg aaatggttcc agtgaaacta gatggttatg caatcgtcat
   221161 catgaatacg aataagcggc gggaattggc agattcaaaa tacaatgaac gccgtagcga
   221221 atgcgaagaa gccttggcac gtttgcaaaa aactttgcct atctcttctt taggagactt
   221281 agatgaagaa gaatttgaat caactattga ccaaatcggc gatgatacat tgatacgcag
   221341 agctcgccat gccgtttctg aaaatcagcg gactttgaag gcaaaagctg aattagaagc
   221401 tgggaactta gaagcatttg gtcaactgtt aaatgattct caccactctc ttcgctatga
   221461 ttatgaagta actgggatcg aattagatac actagtagat gctgctcaaa aacaagaagg
   221521 tgtcttaggt gcacgaatga caggagctgg atttggtggc tgtgcgattg ctcttgtcaa
   221581 agaagaaaat atacctgcct tcaaaaacaa tgtctatgat gaatacatga aagttgtcgg
   221641 ctatgctccg gaattttatg ttgcacacat tggaaatggg acaacaaaga tcgatccaga
   221701 agctgttcat gtttgataaa aaaatcaacg atctcagcta aaaacaaata gataaaaata
   221761 acctctcagc aatttccaaa tgtgagcctt ttgctcgtat ttgaaaattg ctgtttttta
   221821 ttgacacgga aagagaacgt gtgttctaat tgtgttgaag aggtgattgt tatgtgggcg
   221881 aatcaaaatc cggtatttgt ttatgaaaaa gaaccttcaa gagatatttt atgtattgac
   221941 tgcaaatctt tttacgcctc cgtagagtgc gttgaaaagg atcttgatcc ccttacaact
   222001 aaattagtag tcatgtccta tccgtccgat tcgacagaaa cacggggtag cggattaatc
   222061 ttggcttcca gtcctgcagc aaaaaaagct tacggcatca ccaatatcag ccgtgccaga
   222121 gatcttccct tcccctatcc aagtgattta tatattgttg ccccaaggat ggcctattat
   222181 atggaaaaga acagagaaat caattctatc tataaaaaat atgcagacga agcgaaccat
   222241 catgtctatt ccgtcgatga aagcttcctt gatgtaacaa atagcatggc actctttggt
   222301 aaaacaaatg cgtacgacat ggctaaactg atccataaag atgtgtttga gcagaccgga
   222361 atctacacga ctataggaat tggcgataac ccgctacttg ctaaactagc cttagacaat
   222421 ggctcaaaag acggaaagca aatgtttgct gaatggcgtt atgaagatgt accagaaaag
   222481 ttatggaaaa tctcagatat cactgacttt tggggaatcg gcaaacgaac agcaaaacgt
   222541 ctgaaacaaa tgggtataca ttctgtttat gatttagctc acgcggatta ttatcacttg
   222601 aaaagcaagc taggggtcat cggtactcag ctttatgccc atgcttgggg catcgatcga
   222661 agttttttgg gacaagaata tacaccaaaa tcaaaaagta tcggaaatag tcaaatccta
   222721 gatagagatt atacaagacg agaagaaatc gagatcgtta tcaaagaaat ggccgaccaa
   222781 gtcggcactc gcttgagacg atctgcagca aaaacacaag tagtcagctt atgggtagga
   222841 tactctttag gctatcttga tccaaatggc aagaccgggt ttcaccagca aatgaaaatc
   222901 gaagcgacaa atgctagcag agatctagca gatgctttat tgaccatttt ccaccagcat
   222961 taccactacc aggatattcg gcgtgtaggc gtgaactgtt ccgacctcgt atacgcgaca
   223021 ggtttacagc ttaacctttt tgaagatcct gaaaagcaga taaatgaagc gaaagttgat
   223081 tttgttgtcg atacgattag aaaaaaatac ggctttcaat caatcatcca tgcgcatagt
   223141 ctcttgcaag gaggacgcgc catcgcacgt agttctctcg ttggcgggca tgcaggtggt
   223201 atgagtggat tagaaggtgg aaaagatgag acggacagca aaaaaattca ttgactataa
   223261 cgagtatcga gatcggccat ttggattaaa atgggggact gcttttgcga tggaagaact
   223321 agtcaacggt atcaaaaaaa tgaagaagaa gcacgcaaac agcctcttcc taaacaacaa
   223381 atgacattgc aagaaatcga tagtatactg acagaagcct ttttatctcg taaagcagta
   223441 atcattcagc tcaatttaaa agatgaattt ggaagactct tggatgaggt cgaagggcac
   223501 ttcattggtg aagcctatga agattacttt atgattgacc atcagccgat attgtgggaa
   223561 gacgtacggt atattgagtt aaaggaaaca aaaaaatggt ttgtgacatg agaggttgtg
   223621 acagaagtga tcaacttcaa aaaataagaa aggatttttc gaaaattgct tcttaaattt
   223681 ttgtggaatt ttggcttatt tttcgaagaa gcaacttctg gaacaacgta tattagatat
   223741 cagaggttgt gaaaaagtcg cttagttttg agtaacaagc caaggcgctg atttcacaac
   223801 ctctgtctat ctatccatct attcggtaag attcatgcat gacgtcttct ttatttagta
   223861 tgtagagccc tttttcttct gaccattcaa cataaatcag ctgttttaca tctgcatagc
   223921 cttctttctt caagttctcc ataagccatg cttcagtcaa ttgattggac tgaagcactt
   223981 tatgctgaat ttgcccttca tctacaagca ggatcgataa agctttgtct tcatctgatt
   224041 ttttcaatac actcatttga ccggcattct ctaggataac atgtttggca ttttcaagaa
   224101 aaaagcattc ttgctgtctt aagattgctc gcaactgctc catctcgata tggttatttg
   224161 tcaactctgt catgttgatg acgccgtctt tgattagaac agatggctcg cctttgaccc
   224221 aacgattgac cttttcattt ttttggacga tacgttcgat accataaatc attactgccc
   224281 aaagcgcaat ggcaaatagg acatgcccta catggacatt gtcatcatag gttgattctt
   224341 ctaagatccc acctaaaaca agcgtataga ctaaatcaaa cggtgtgatt tctgacatgg
   224401 atttcttccc aagcaaacgt accactaata ataaaccgat caacccgaac accagtttca
   224461 ttgtaattga aaaaatcatt ttttctcccc ctaactatct gttaattaaa ttatatatgc
   224521 tttctcacga acacgcatgg aatatacaag gaagaaatgt atccttttac taaaaaaagg
   224581 actgtgacac tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactcc
   224641 ttttttttat gctctaaaat gatattttta cacaaccgtt acggttgttt tacgaaattg
   224701 gacg
//
LOCUS       DAPBCS010000002        93455 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPBCS010000002 DAPBCS010000000
VERSION     DAPBCS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 93455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.002"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            85..972
                     /locus_tag="RD521_000205"
     CDS             85..972
                     /locus_tag="RD521_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7769972.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="RD521_000206"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="RD521_000206"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDT7769973.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="RD521_000207"
     CDS             2274..2720
                     /locus_tag="RD521_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7769974.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="RD521_000208"
     CDS             2993..4345
                     /locus_tag="RD521_000208"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDT7769975.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="RD521_000209"
     CDS             4516..4803
                     /locus_tag="RD521_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769976.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="RD521_000210"
     CDS             5029..5472
                     /locus_tag="RD521_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7769977.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="RD521_000211"
     CDS             complement(5478..6662)
                     /locus_tag="RD521_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDT7769978.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="RD521_000212"
     CDS             6930..7628
                     /locus_tag="RD521_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7769979.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="RD521_000213"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="RD521_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDT7769980.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="RD521_000214"
     CDS             complement(8438..8971)
                     /locus_tag="RD521_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769981.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYSPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="RD521_000215"
     CDS             complement(9268..11649)
                     /locus_tag="RD521_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDT7769982.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="RD521_000216"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="RD521_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDT7769983.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="RD521_000217"
     CDS             12350..12889
                     /locus_tag="RD521_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDT7769984.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="RD521_000218"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="RD521_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDT7769985.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="RD521_000219"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RD521_000219"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="RD521_000220"
     CDS             13996..15162
                     /locus_tag="RD521_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDT7769986.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="RD521_000221"
     CDS             15208..16704
                     /locus_tag="RD521_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDT7769987.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="RD521_000222"
     CDS             16785..17468
                     /locus_tag="RD521_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7769988.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="RD521_000223"
     CDS             18113..19303
                     /locus_tag="RD521_000223"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDT7769989.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="RD521_000224"
     CDS             19405..20358
                     /locus_tag="RD521_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDT7769990.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="RD521_000225"
     CDS             20427..21776
                     /locus_tag="RD521_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7769991.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="RD521_000226"
     CDS             complement(21959..22282)
                     /locus_tag="RD521_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7769992.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="RD521_000227"
     CDS             22411..23574
                     /locus_tag="RD521_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7769993.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="RD521_000228"
     CDS             23644..24993
                     /locus_tag="RD521_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7769994.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="RD521_000229"
     CDS             complement(25038..25469)
                     /locus_tag="RD521_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7769995.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="RD521_000230"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="RD521_000230"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDT7769996.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="RD521_000231"
     CDS             28699..29295
                     /locus_tag="RD521_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7769997.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29417..30565
                     /locus_tag="RD521_000232"
     CDS             29417..30565
                     /locus_tag="RD521_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7769998.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30603..31016)
                     /locus_tag="RD521_000233"
     CDS             complement(30603..31016)
                     /locus_tag="RD521_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDT7769999.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31095..31526
                     /locus_tag="RD521_000234"
     CDS             31095..31526
                     /locus_tag="RD521_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDT7770000.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31625..31858)
                     /locus_tag="RD521_000235"
     CDS             complement(31625..31858)
                     /locus_tag="RD521_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDT7770001.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32041..33711
                     /locus_tag="RD521_000236"
     CDS             32041..33711
                     /locus_tag="RD521_000236"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDT7770002.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33858..34244
                     /locus_tag="RD521_000237"
     CDS             33858..34244
                     /locus_tag="RD521_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770003.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34231..34929
                     /locus_tag="RD521_000238"
     CDS             34231..34929
                     /locus_tag="RD521_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7770004.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34934..35398
                     /locus_tag="RD521_000239"
     CDS             34934..35398
                     /locus_tag="RD521_000239"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDT7770005.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35395..36306
                     /locus_tag="RD521_000240"
     CDS             35395..36306
                     /locus_tag="RD521_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7770006.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36541..37080
                     /gene="pyrR"
                     /locus_tag="RD521_000241"
     CDS             36541..37080
                     /gene="pyrR"
                     /locus_tag="RD521_000241"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDT7770007.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37094..38377
                     /locus_tag="RD521_000242"
     CDS             37094..38377
                     /locus_tag="RD521_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDT7770008.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38390..39316
                     /locus_tag="RD521_000243"
     CDS             38390..39316
                     /locus_tag="RD521_000243"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDT7770009.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39335..40615
                     /locus_tag="RD521_000244"
     CDS             39335..40615
                     /locus_tag="RD521_000244"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDT7770010.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40620..41696
                     /gene="carA"
                     /locus_tag="RD521_000245"
     CDS             40620..41696
                     /gene="carA"
                     /locus_tag="RD521_000245"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDT7770011.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41701..44886
                     /gene="carB"
                     /locus_tag="RD521_000246"
     CDS             41701..44886
                     /gene="carB"
                     /locus_tag="RD521_000246"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDT7770012.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44940..45716
                     /locus_tag="RD521_000247"
     CDS             44940..45716
                     /locus_tag="RD521_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDT7770013.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45713..46645
                     /locus_tag="RD521_000248"
     CDS             45713..46645
                     /locus_tag="RD521_000248"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDT7770014.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46642..47358
                     /gene="pyrF"
                     /locus_tag="RD521_000249"
     CDS             46642..47358
                     /gene="pyrF"
                     /locus_tag="RD521_000249"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDT7770015.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47365..47994
                     /locus_tag="RD521_000250"
     CDS             47365..47994
                     /locus_tag="RD521_000250"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDT7770016.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47982..48296
                     /locus_tag="RD521_000251"
     CDS             47982..48296
                     /locus_tag="RD521_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770017.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48334..48975
                     /locus_tag="RD521_000252"
     CDS             48334..48975
                     /locus_tag="RD521_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7770018.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49018..49710
                     /locus_tag="RD521_000253"
     CDS             49018..49710
                     /locus_tag="RD521_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDT7770019.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            49707..50609
                     /locus_tag="RD521_000254"
     CDS             49707..50609
                     /locus_tag="RD521_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7770020.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="RD521_000255"
     CDS             complement(50767..52473)
                     /gene="efbA"
                     /locus_tag="RD521_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDT7770021.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52663..53286
                     /locus_tag="RD521_000256"
     CDS             52663..53286
                     /locus_tag="RD521_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770022.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53736..54785
                     /locus_tag="RD521_000257"
     CDS             53736..54785
                     /locus_tag="RD521_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7770023.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54782..55663
                     /locus_tag="RD521_000258"
     CDS             54782..55663
                     /locus_tag="RD521_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770024.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55666..56436
                     /locus_tag="RD521_000259"
     CDS             55666..56436
                     /locus_tag="RD521_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7770025.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56715..57569
                     /gene="aroE"
                     /locus_tag="RD521_000260"
     CDS             56715..57569
                     /gene="aroE"
                     /locus_tag="RD521_000260"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDT7770026.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57747..58910
                     /locus_tag="RD521_000261"
     CDS             57747..58910
                     /locus_tag="RD521_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7770027.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59027..59437
                     /locus_tag="RD521_000262"
     CDS             59027..59437
                     /locus_tag="RD521_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7770028.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59611..61839
                     /gene="topB"
                     /locus_tag="RD521_000263"
     CDS             59611..61839
                     /gene="topB"
                     /locus_tag="RD521_000263"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7770029.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            62010..62294
                     /locus_tag="RD521_000264"
     CDS             62010..62294
                     /locus_tag="RD521_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770030.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            62454..62690
                     /locus_tag="RD521_000265"
     CDS             62454..62690
                     /locus_tag="RD521_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7770031.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62687..63086
                     /locus_tag="RD521_000266"
                     /pseudo
     CDS             62687..63086
                     /locus_tag="RD521_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(63753..64697)
                     /locus_tag="RD521_000267"
     CDS             complement(63753..64697)
                     /locus_tag="RD521_000267"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDT7770032.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(64993..65937)
                     /locus_tag="RD521_000268"
     CDS             complement(64993..65937)
                     /locus_tag="RD521_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770033.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            66158..67372
                     /locus_tag="RD521_000269"
     CDS             66158..67372
                     /locus_tag="RD521_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7770034.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            67394..69409
                     /locus_tag="RD521_000270"
     CDS             67394..69409
                     /locus_tag="RD521_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDT7770035.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(69492..70865)
                     /locus_tag="RD521_000271"
     CDS             complement(69492..70865)
                     /locus_tag="RD521_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7770036.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(70891..71175)
                     /locus_tag="RD521_000272"
     CDS             complement(70891..71175)
                     /locus_tag="RD521_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7770037.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(71177..73192)
                     /locus_tag="RD521_000273"
     CDS             complement(71177..73192)
                     /locus_tag="RD521_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7770038.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(73371..73676)
                     /locus_tag="RD521_000274"
     CDS             complement(73371..73676)
                     /locus_tag="RD521_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDT7770039.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(73765..74796)
                     /locus_tag="RD521_000275"
     CDS             complement(73765..74796)
                     /locus_tag="RD521_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7770040.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            75003..75878
                     /locus_tag="RD521_000276"
     CDS             75003..75878
                     /locus_tag="RD521_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7770041.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(76030..76872)
                     /locus_tag="RD521_000277"
     CDS             complement(76030..76872)
                     /locus_tag="RD521_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7770042.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(76964..78841)
                     /locus_tag="RD521_000278"
     CDS             complement(76964..78841)
                     /locus_tag="RD521_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7770043.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(78838..80259)
                     /locus_tag="RD521_000279"
     CDS             complement(78838..80259)
                     /locus_tag="RD521_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDT7770044.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(80262..83651)
                     /locus_tag="RD521_000280"
     CDS             complement(80262..83651)
                     /locus_tag="RD521_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDT7770045.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            83908..85299
                     /locus_tag="RD521_000281"
     CDS             83908..85299
                     /locus_tag="RD521_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770046.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(85372..86019)
                     /locus_tag="RD521_000282"
     CDS             complement(85372..86019)
                     /locus_tag="RD521_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDT7770047.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(86027..86713)
                     /locus_tag="RD521_000283"
     CDS             complement(86027..86713)
                     /locus_tag="RD521_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770048.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(86719..87792)
                     /locus_tag="RD521_000284"
     CDS             complement(86719..87792)
                     /locus_tag="RD521_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770049.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            88014..88688
                     /locus_tag="RD521_000285"
     CDS             88014..88688
                     /locus_tag="RD521_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7770050.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            88693..90033
                     /locus_tag="RD521_000286"
     CDS             88693..90033
                     /locus_tag="RD521_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7770051.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(90182..91168)
                     /locus_tag="RD521_000287"
     CDS             complement(90182..91168)
                     /locus_tag="RD521_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDT7770052.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            91892..92923
                     /locus_tag="RD521_000288"
     CDS             91892..92923
                     /locus_tag="RD521_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7770053.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            93032..>93455
                     /locus_tag="RD521_000289"
     CDS             93032..>93455
                     /locus_tag="RD521_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDT7770054.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        29895 a        16575 c        18966 g        28019 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggagaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtggaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaaat aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaagaac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaaca atcgtcctct ccatcttttt acctgagcaa caagcaaact
    29401 agaaggggta aaaaacttgg ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca
    29461 gcggacaaaa aaaggagtac tagcgggtat cttaggattg gttacgaatc tgatcttgtt
    29521 tgtctctaag ctaatgatcg ggcttttttc aggaagtgtc tccattatgg ctgatgcagt
    29581 gaacagtcta tcagatactg cttcatccat attgactttg atcggattta aaattgctgc
    29641 aaagccagca gatcaggaac atccttatgg acacgaacgc ttcgaatata tcagtgggtt
    29701 atttgtttcg atcatcatta cttatgtagg atttcaattt ttagagtctt ctgctgaaaa
    29761 aatcatccat ccagaaaatg tctctttaac acccatcgtc ttttttgtcc taatcatttc
    29821 catctttttg aaatttgcac aaggcaaaat gtatcaggtt attgcgcaat ccattgattc
    29881 tgaaacattg agagctacag caacagatag ttataatgat gtctttacta cactggctgt
    29941 attgctttca gcagcaatcg aatgggtaac tggatggcgt attgatggat atataggttt
    30001 tgctttagca ttatatatcc tttatagtgg attgacgatg cttcgaaatt ttgtatacga
    30061 actattagga agtcgcccaa cgccagcaga gattgaagaa atggaagagc atttaaatgc
    30121 ttgcgatgat atacttggct atcatgattt attggtgcat aactatggac ccaataaacg
    30181 ctttgcatca gtccacatcg aagtagatga atcaatgaat ttgaatgtgg ctcataagat
    30241 tattgatctg atagaaaaag actttaaaaa gtctttggat gtggaacttg tctgtcactt
    30301 ggatccaatg cctgttgata acgaaaaata ctataatatc ttgcggcagt taaagcaaat
    30361 tcttcttgaa acaggtacag ggcttaatat tcatgatttt agagtaattc atcaaggaaa
    30421 aacgctgcaa tttgatgtag tggtccctga gaaattccag tttgttgata cagaactaga
    30481 aagaatctta cgtagtcaaa tagaagaaaa aatcggagat taccaattgg aaattacgtt
    30541 tgatcacaat tatcttttat attaaatgag aaactataaa aagactcctt aaagttttta
    30601 atttaaagag tctttttttc ttttaattgt ttatggagag cctgacgtgc aagattatct
    30661 gctcctttat ttttgttttc gggcaaccat tgcaggatca ataaagaaaa ttcggataga
    30721 agttcttgga ttctttgaag gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg
    30781 accgtagaaa ctagtgtttt gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg
    30841 tttttcttta gaaattctaa agtttttaaa aagacttcga actctgcttg atggtttgtc
    30901 cctatagaaa gcgggataga tagctgttct tgtttacctt gatagacaat caagattcca
    30961 cctccactag gaccagggtt gccttttgta gaggcatcaa tgtatacttt taacataaaa
    31021 agagattctc ctttttgttc aaactaatgt tatggtaaga ttatatcatt actgaatcgg
    31081 tgagaggtta gagaatgaaa aaaaatattc gatggcagcc tgaactggca catacgatca
    31141 tctattggtc attgaccttg atcattctgt tttttagtct gattctttct ttagaaaaca
    31201 caggtcctta ttggaaaagc aattttgttt taggtatttt tcttgttttt ctatttctag
    31261 gtttgcggcg ttcttttatt ttaaaatcaa actctgtgat tatacgttat gctgcttttt
    31321 ggaaagaccg agaaatatct gtcggacaga ttcgatcttt gacgccaact aatactgggg
    31381 tgcaaatgat gttgcaagat gagaaagccc catctgtttt catgatgcga aaaaaagatc
    31441 aagtagtcct ttttgatcat tttgaaaaaa acggacccaa atcagaatca attccttatc
    31501 cttatacgaa aaaaagaaaa agctaatttt aaaaatcaga acctaaaaaa acgaattttt
    31561 acaggaaaag aaaagagtga gacgtacggt tcatcccgtt tagcgtctca ctctatttgt
    31621 ttatctattc atgcatacta gtatcggtag tctgaacgtt gcctttcttg acatgggcag
    31681 cttgtgttcc tcgattccct tcgatgatct caaattcgac cacttgattt tcttccaaag
    31741 ttttgaatcc ctcttcttca atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat
    31801 aactgataaa gccgtatcct tttttgttgt caaaccattt tacaattcct gtggtcaaag
    31861 cactcacgac ctttctaaag tctgatcata gccttcatta taaagagtat ttataagtac
    31921 gtcaaataaa ttaaaaacct gtaattttat gttttttttc tgaataaaaa aaggtataat
    31981 tggcatatct taacaaagaa gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa
    32041 atgaagaatg atctgcaaat ttcacaagaa acagagctag aaccgattgt tgaagtggct
    32101 agtaaattag atcttaagga agacgatttg gaactgtatg ggaaatataa ggcgaaaatc
    32161 aatttttctg gaatcaatcg atcaaaggat aacgctgagg ggcatttgat cttagttact
    32221 tccatcaatc caacaccagc tggcgaagga aagtcaacga tcactgtagg gttaggagac
    32281 gcgctcaata aaatcaataa aaaatctgtt attgcattgc gagaaccctc acttggtcct
    32341 gtgatgggaa tcaaaggtgg tgctgcaggc ggaggatatg cacaggtatt gcctatggaa
    32401 gatatcaact tgcattttac tggtgacatg catgctatca caacagctaa taatgcatta
    32461 tctgctcttt tagataatca tattcatcaa ggaaatgaat tgaacatcga tgcccgccga
    32521 gtgatttgga aacgggtagt cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc
    32581 ttgggtgggc caatccaagg tgttccaaga gaagatgggt ttgatatcac agttgccagc
    32641 gaaatcatgg ctatcttgtg tcttgcggca gaccttgaag atttaaaggc tcgtcttgca
    32701 aggatcgttg tcggctatac atttgaccgt cagcctgtga ctgctggaga cttgaaagca
    32761 gaaggcgctc tggcattact gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt
    32821 tacggaacac cagcttttgt ccacggggga ccgtttgcca atatcgctca tggctgcaat
    32881 agtattttag ctaccaagac agcattgaga ttagcagatt acgttgtcac agaggcagga
    32941 tttggtgcag atcttggcgg agagaaattt ttagatatca aagtacctaa cttgaaaaaa
    33001 gcgccggatg ctgtggtgat tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg
    33061 aaaaaagatg aacttaaaaa tgagaatctt gatgctttga aagtaggttt tgcaaattta
    33121 aagcgccata tacgtaatat ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa
    33181 ttcgtaacag atacagacaa tgaactcaca ctgttggagc acttgtgtga aaatcagggg
    33241 attcttgcca agagagcgag tgtatgggca gatggagcag aaggcggcgt tgatttagca
    33301 aaagctgtag tacgattgat tgatagaaaa gaagctaatt acaagccatt ataccgccta
    33361 gaagaaacga tacaagaaaa gacagaaaca atcgtcaaga aaatttacgg aggaaatggt
    33421 gttgttttca gcaaaaaagc gcaaaaacaa atagaagagt ttacgaagaa tggatgggac
    33481 aaactgccga tttgtatggc aaaaacacag tattcatttt ctgatgatcc ttcactgcta
    33541 ggtgcaccgg aagatttcac catcacgatc cgcgaaatca tcccaaaatt aggggcagga
    33601 tttttagtag ccttgactgg agatgttatg acgatgcctg gtttgcctaa aaaacctgct
    33661 gcattgaata tggacgtgac aaatgacgga gaagtgttgg gattgtttta agcttgatta
    33721 taatctacca tcgaaggagc tgtattttga ctacagcttc tttttttaga cgatagggaa
    33781 ttaattttgt ttacactagg atcattcttt ttctttattg tattttgagc tatactaaag
    33841 cataatagga gggtatgatg aaaacgacat ttataagaaa aaccggtttt tacggtatgg
    33901 gtagctcgat tcagctaaga atcaacaatg aaaaacgcag tgttctcagt catgaacaaa
    33961 agataacgct tgatctggaa gtgcccttta cgatgcaggt caccttttat tggctaaaaa
    34021 gccccgttta tacagtgaaa gaagcaaagc ctgcttatgt gatcacaatg aattacttga
    34081 ttcttcaaat ctatccgttt ttatttctag caactgggtt gggggccgtt ttcctacaaa
    34141 atttattttt cagttttttt gcagttattg tgttgattgc gttctttttc tatataaaaa
    34201 accgggcata tttgatcaag gaggaaaatt atggcaaact ctgatctttt tttaatcagt
    34261 ttcaatcgaa tcgaaaaatg gatgagagac gagatgggaa atgcacgaaa tatgggattt
    34321 accgaacttg tcagacgcct agctcaaaga aaacaattaa tgatcagaaa atacgaagat
    34381 gatcttttac agcttgctca gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat
    34441 tttgtcattg cagagccaaa tgattggaca gtcaagcgaa tccagctgat cgaacaagaa
    34501 ctgattcagc ctgaaaccgt gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa
    34561 gatcttccgt tacttgaact gttaaagata gtagcagaaa aaaggtattc acaatttccg
    34621 ttatacaata aaggaaaatt tgtagcattg attactttac gtaatatcgg gttttggcta
    34681 gcaaaagaaa gccaaaaagg tccggttgat ttaacaaata aacgagcgtt ggatttgatc
    34741 atccaaaacg gaaagtacac aaattatcat tttgttccag ctaaaacaca tatttacgag
    34801 gttgaagcga tgtttcgtga gcaagggact ttggaagccg tacttatcac taaagatggg
    34861 aatcccgatg gtaatctgct cgggatcgta cgacctcgtg atatttataa acaaatagaa
    34921 aaggattgat tttttgttaa ttatttattg gatattcagc gctttgctag taggagtgga
    34981 tcagttggtc aaatggtgga tcactgataa tctagcgctt ggagagacga aaaatcttat
    35041 accaggtatt ttgtcactga atcatattcg aaacactgga gccgcatgga gtatgctaga
    35101 agggaagatg tggttcttta ctatagtgac actcattgca gtcgttgtga ttctcacatt
    35161 gatgattaaa aatcgtgaaa aaggtaatcg ctggttcatg attggattga gtttgattct
    35221 agctggtgcg attggcaatt ttatcgaccg tattcgtctt ggctatgtag tagatatgtt
    35281 ccagacagat tttatgagct ttccgatttt taacgtagca gatgtgacac ttgtatgtgg
    35341 agtgatttgt atgttaattt atattatttt agatgaaaag gatcaaagaa aaaaatgaca
    35401 gaacttaaaa cgacgattca ccatgaatct ggtaggatcg ataaagtatt gactaattta
    35461 ttcaccgatt acagtcgttc gcaaattcag ctgtggctga aaaatggagc ggtttctgtc
    35521 aatggacagc cagtcaaagc aaattacaaa gtaaaacaag gagacgagat tactgtcttg
    35581 atccccgaac cagaaacatt agatgtggtc ccagaaaata tcccgttgac gatcgtttac
    35641 gaagatgaag atgtagcggt gataaacaaa cctcaaggaa tggtcgttca tccttcagca
    35701 ggtcatacgc atggcacaat ggtcaatgcg ctgatgtacc atatcaagga tctttctacg
    35761 atcaacggcg ttattcgtcc aggtattgtt caccgaatcg acaaagatac ttccggactg
    35821 ctaatggttg ccaaaaatga ccaggcacac gaatcattag ctcagcaatt aaaagataag
    35881 acctctttgc gcaaatatgt tgctttagtc catggagaga ttcctcacga aaaaggaacc
    35941 atcaatgcgc caatcggccg ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat
    36001 gggaagtcgg ctgtcacaca ttttactgta ttggaacgtt tcgaaggatt tactctggtt
    36061 gaattaaaac tagaaaccgg aagaacacac cagatccgtg tgcatatgaa gtatatcggt
    36121 tatccgttag ctggagatcc agtatacgga ccgaagaaaa ctttgaaagg caatggtcag
    36181 tttctccatg cgcaaatgtt aggttttacg cacccgacta caaacgaaaa gatgatcttt
    36241 gaagcacctc ttccagaagt ttttgaaaaa actttagaaa aattaagaaa aaacattgca
    36301 ttttaattta gataacggta tagtaattaa caacgaaagc ataaaactta atagaattcc
    36361 tttaagttta gtcccgtgag gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat
    36421 aagtagattg ctcattgaca tagtatagct atcagtgtat cagtatacgc tcagtctaaa
    36481 cattcctcct gtcctcttag ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa
    36541 atgcaagcaa aagaagtagt agatcaggta accatgaagc gagcattgac tcgaattact
    36601 tatgagatta tcgagagaaa ccatagtatc caagatatcg tattggtagg tatcaaaaca
    36661 cgaggaatct acattgcatc aagaattgcc gaacgcctga agcagctaga agatatcgat
    36721 attcctgtag gagaactgga tatcacttta taccgagatg acaaaaaaga aaatccagaa
    36781 gaaccggaac ttcattcttc tgacatccca gtttccttag aaggaaaaga agttattttg
    36841 attgatgatg ttttgtatac aggacgaacg attcgagcag caatggacgc agtaatggat
    36901 tttggcagac caagaaaaat ttcattagca gtcctggtcg atcgtgggca tcgagaattg
    36961 ccaatccgtg cagattatgt tgggaagaac attccaacat ctcgggcgga agagatttta
    37021 gtagagatgc aagagcttga tggacaagat cgcatcatga ttttgaaaga ggaagattag
    37081 aggagagaga aacatgtcgg agaaaaaagg atttcataat gatgacgtgg tgttagatat
    37141 ccacgatcgc ccaaccccag gacattgggt aggattaagt ttacaacact tgttcacgat
    37201 gttcggagca accgtgttag tccccatctt agtaggtatc gatccaggta ttgcgctagt
    37261 cagttcagga ctaggtacat tggtgtactt atttactact aaaggaaaga tacctgcgta
    37321 cttaggaagc agcttcgctt tcattgcagc gatgcagctt ttgatgaaaa cagatggtta
    37381 tccagcgatt gcacaaggag cagtcacaac tggattagtc tatttgattg ttgcgctgat
    37441 catcaaaaag atcggttcag catggctgga taaaatcttg cctccaatcg tcgtcggtcc
    37501 agtcatcatg gttatcgggt tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa
    37561 tcagggcacg tatgatttca agtatattgc tgtagcgctg atcacattag gattaacaat
    37621 tttcttcaac atgtggctaa gaggttttct cggattgatt ccgattttac taggtattat
    37681 tagcggatac atcgtagcct tgctgtttgg aatcgtggat actaagccaa tcgtagacgc
    37741 tgcctggttt gctgttccta actttgagat tccctttgta caatatgaac caaagctata
    37801 tcttggagca atcacaacaa tggcacccat cgcttttgtg acgatgacag agcatatcgg
    37861 ccatcttatg gtattgaata aactaactaa acgtaatttc tttgaagaac cgggtcttca
    37921 taagacgttg acaggtgatg gattagcaca aatcgtggca gcctttgtcg gaggtcctcc
    37981 tgtaacaagt tacggtgaaa acattggtgt tctggctatc acacgtgtcc atagcgtgtt
    38041 tgtgatcggt ggagctgcag tatttgcagt cattttagga ttcgtaggaa aattaagcgc
    38101 tttgatttta agtatccctg gaccagtcat ctcaggaatc agttttatct tatttggagt
    38161 aattgccgca agtggaatga agattttaat cgagaacaaa atcgattttg ataaaaagaa
    38221 aaatctgttg attgcttcag ttatcttagt agtaggaatt ggcggactcg tctttgaagt
    38281 aggcacattt acattgtcgg caatggcatt agcaactgta cttggcatca ttttaaatct
    38341 tatcttacca gaaacttcac gaagcgaaga aaactaagga ggaaacatta tgatcatcaa
    38401 atcagaacgt atcagcttaa agcatttatt gaccgttgag gcacttactg atcaagaagt
    38461 tatgggcttg atccgcagag ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa
    38521 gcgccagtac tttgctacaa acctcttttt cgaaaatagt actcgcacac ataaaagctt
    38581 cgatgtagcg gagaaaaaac tagggctaga agtgattgaa tttgaagcaa gcacaagctc
    38641 tgtccaaaaa ggggaaacac tatacgatac agtactgacg atgtcagcat taggcgtaga
    38701 cgtagcggtt atccgtcatg gtgatgagaa ctattatgac gaactgatac aaagtaaaac
    38761 aatccagtgt tcgattatca atggaggaga cggaagcgga cagcatccaa cccaatgctt
    38821 gcttgatttg atgaccattt atgaagaatt tggtcatttt gaaggtctga aagttgccat
    38881 cgtaggtgat atcactcact ctcgcgtagc taaatccaat atgcagatgt taaaacgtct
    38941 aggcgcacaa gtattcttct caggtcctag agaatggtac gacgatgaat tcgaagtgta
    39001 cggacattat atgccattgg atgaactact agatcaagtt gatgtcatga tgatgcttcg
    39061 ggtgcaacac gaacgccatg atggaaaaga aagtttctca aaagaaggct accatcagga
    39121 acatgggtta acagttgagc gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc
    39181 agcaccagtt aaccgtgatg tagaattagc tgattcatta gtggaaggat tacagtcgcg
    39241 gatcgttcag caaatgagca atggagtatt cgtcagaatg gcaatcttag aagcagtgct
    39301 aaatggaaaa gcgtaattat aggaggatga aaaaatgaaa acactcatta aaaacggtca
    39361 gatcaataca cgaaaaaata tgacgacacc agcagaaatt tggatagaag atgggcgaat
    39421 caaagcaatc ggtacaggat tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg
    39481 acaattgatc acacctggtt tagtggacgt ccatgttcat ttgagagagc caggatttac
    39541 atacaaggag acgatcgaag ctgggactcg ctcagctgct cgtggcggtt tcactactgt
    39601 atgcgcgatg ccgaatttaa atcctgtacc tgatacggca gaaaagctga gacaagtata
    39661 tgacatcatt agaaaagatg cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa
    39721 tctgcgaagt gagaaattag ttgatcaaga agcactgatt gaagaaggtg cttttgcttt
    39781 cacaaatgac ggtgtaggtg tccaaacagc tgggacgatg tatctggcta tgaaagaagc
    39841 tgcaaaaaac aataaagccc tagtagccca tacagaagat gaatctttat tatttggcgg
    39901 cgttatgcac gctggcaaga aggcagaaga acttggtctt cccgggatct taagcgtaac
    39961 cgagtcttct caaattgcta gagatctgtt gctagctgaa gcaactggtg ttcattacca
    40021 tgtttgccat gtttctacta aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg
    40081 aatccacgta acagcagaag tttcacccca tcatcttatt ttgatcgacg aagatattcc
    40141 cgaagatttc ggcttttgga aaatgaaccc gccgctaaga ggacgggagg acagagaagc
    40201 tttgatcgaa ggcttgcttg acggtacgat cgattgcatc gctacagacc atgcccccca
    40261 tggattagaa gaaaaaagcc aaagtttcat gaaatcacca tttggtatcg taggaagcga
    40321 aacagcgttt caactgatct atactcattt tgtagaaact ggtcgattca cgttggaaca
    40381 agtgatcaat tggttggcag ttaaaccggc tgagattttt ggtttgaatg caggaacgct
    40441 aacggttggt gcccccgctg atgtagcagt ttttgatatt acacaaactt gcacgatcga
    40501 taaagaagac tttttatcaa aaggggagaa tacacctttt atcggctgga aagtaaaagg
    40561 cgaaacacaa atgacttttg tcaacggaaa acttgtgtgg cagaagggag aataaaagta
    40621 tggaacgttt gcttatatta gaagatggaa cagttttcga aggaaaggct tttggtgcag
    40681 atgctagtgt cttcggagaa atcgttttta caacaagtat gactggttat caagaaacta
    40741 tcactgatca aagttttaac ggtcaaatca ttacatttac ctatcctatg gtgggtaatt
    40801 atggggtaaa tagagatgat tatgaatcga tttctccaac atgcaaagga gtagtagtaa
    40861 aagaacatgc acgtgtagct tcaaactggc gaaaccaaat gacgctagat tcatttttga
    40921 aacaaaaagg aattcctgga atatcaggta tcgatacgcg cgcccttact cgcaaaatcc
    40981 gtgaagtcgg aacgatgaaa gctagtatag ttgatgctgg agattccttc gaacatgcat
    41041 ttgatcagct gaaagcaacc gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca
    41101 aaccttatcc aagtccagga acaggtcgga aagtcattgt cattgacttc ggcttgaaac
    41161 atagtatttt acgagaattg agtaaaagaa actgtaacct cacagtactt ccctataaca
    41221 cagatgccga gacgatttta gcgctatcac cagacggagt catgctgacg aatggacctg
    41281 gtgatccaaa agatgtgcca gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac
    41341 cgatttttgg tatttgtctc ggtcaccagt tatttgcttt agccaacggt gcagatacct
    41401 ataaaatgaa attcggacat cgaggattaa atcacccggt acgagaagta gcgaccggaa
    41461 gaattgattt tacttctcaa aatcatggct atgcagtcga tgaagccacg attgatccag
    41521 aaaaactcat cgtaactcat gtagaagtaa atgacggaac agtagaagga gtcaaacaca
    41581 gagattatcc cgctttcagt gtgcagtttc atccagatgc tgcccctggt ccgcatgatg
    41641 cactacattt atttgatgaa tttatggaaa tgatggacgc aggaaaggag cagtaaacag
    41701 atgccgaaac gtacagatat caagaaaatt atggtgattg gatctggtcc gatcatcatt
    41761 ggtcaagccg cagaatttga ctacgcagga acacaagcct gccttgcctt aaaagaagaa
    41821 ggatatgaag tggttttagt aaattctaat cctgcgacga tcatgacgga taaagaagtg
    41881 gcagatcaag tatatatcga accgatcaca cttgaatttg tatcaaggat tcttcgaaaa
    41941 gagcgtccag atgctctcct tccaacttta ggcggacaga caggcctcaa tatggcaatg
    42001 gagctggcag catcaggtat tttagaagaa ttgaacatag aacttctagg aacaaagctt
    42061 tctgcgatcg atcaagcaga agatcgtgat ctattcaaac agttgatgaa agaattgaat
    42121 cagccaatcc ccgagtctga aatcgtcaat acagtagaag aagctgtaga ttttgcaaat
    42181 aaaatcggct atcctgtcat cgttcgtcca gcctttactt taggcggaac tggtggaggt
    42241 atgtgtgaaa atgaagaaga attacgtatc gtatcagaaa atggattaag tctttccccc
    42301 gctacccaat gtttgattga acgaagtatt gcaggtttta aagaaattga atatgaagtg
    42361 atgagagatt cggctgataa tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg
    42421 ggtatccata caggtgattc tatcgtattt gcccctagtc aaacattgtc tgaccatgaa
    42481 tatcaaatgc ttcgtgatgc ttctctttcg attattcgag cacttaagat cgagggaggc
    42541 tgtaatgtcc aactcgctct tgatccacat agttttaatt attatgtgat tgaagtcaat
    42601 ccgcgtgttt ctcgttcatc tgcgttagca agtaaggcga ctggctatcc gatcgctaag
    42661 ctagctgcta aaatagcgct tggattaaca ttagacgaaa tgaaaaatcc tgttacagga
    42721 acgacttttg cagaatttga acctgcatta gattatgttg tagctaaaat tcctcgctgg
    42781 ccgtttgata agtttgaaaa cggagaacgg cttttaggca cgcagatgaa agcaactggt
    42841 gaagtgatgg ctatcggtcg gaatatcgaa gagtctttat tgaaagctgt gcgttcttta
    42901 gaaatcggta cacaccatat cgaacttcct gagttggcag acatcacgga cgatgaccta
    42961 atccaaaaag tggttcgagc acaagatgat cgcctgtttt atgtagctga agctattaga
    43021 agagggtaca ccatagaaga attagcggat ttgacaaaaa tcgatttatt ctttttagat
    43081 aaactgttgc atatcgtaga aatcgaacag cagttgaaac agcagccgtt ggatgacaag
    43141 atattgaaaa cagcgaaaca aaacggtttt acagacaaaa aagtggcgga gctgtgggaa
    43201 ataaacgaag aagccgttcg ccagatgaga aaagaaaatg atttgattcc tgtatataag
    43261 atggttgaca cttgtgcagc ggaattcgaa tcaaaaacac cttattttta tagttcctat
    43321 gaatatgaaa atgaaagttc acgatcagaa aagccctcaa ttctagtact gggatctggt
    43381 cctatccgta tcggacaagg ggttgaattt gactatgcga ctgtgcattc tgtcaaagca
    43441 atccagcagg caggatacga agcaatcatc atgaacagca atcctgaaac agtttcgact
    43501 gacttttcta tttcggataa attatatttt gagccattga ccttcgaaga tgtgatgaat
    43561 gtgatcgagc tagaacagcc gataggagta atcgttcaat ttggtggtca gacagcaatc
    43621 aacttagccg aacctttgac tgctgctggt gtaaaaatct tagggacaac gatcgaagac
    43681 ttggatcgtg cggaaaatcg tgatttattc gagcaagcgc tgaaagagtt ggatattcct
    43741 caacctttag gggatactgc tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt
    43801 ggttatccag ttcttgttcg tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt
    43861 gaaaaccagc gagatttaga agattacatg gaacatgctg tgaaagcatc tccagaacat
    43921 ccagtactag tggaccgtta cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac
    43981 ggtgaaacag tactcatccc agggatcatg gagcatattg agcgtgctgg tgtccattca
    44041 ggggattcaa tggctgtgta tccacctcaa acattatctg aatcgatcaa gaaaacgatc
    44101 gaagattaca cgatccgctt agcattaggt ttgaattgtg tcgggatgat gaatatccag
    44161 tttgtcatcc atgaaaatga ggtttatgtg atcgaagtca atcctcgagc aagtcggact
    44221 gttccattct taagtaaggt gacaggaatt ccaatggcac aaattgcgac gaaagctatt
    44281 ttgggagaga aactaaagac acttggttat gaaaacggac tttatccaga aacaaaagaa
    44341 gttcatgtta aagcacctgt cttttctttt acgaaattgc agaaagttga tacttactta
    44401 gggcctgaaa tgaaatcgac tggagaagta atgggatctg acagacaatt agacaaagct
    44461 ttgtacaagg cattcgaagc ttctggatta cacttgcctg aatatggagc cgtattattt
    44521 acgattgcgg acgaaacaaa agaagaagca ctagaattag ctaaacgttt tacagaaatt
    44581 ggctatagtc tgataggaac gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc
    44641 gttgaaacag tagcgaaaat ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata
    44701 agagatggta aaacacaagt ggttgtcaat acgattgata aagatcgggg aaatgcttct
    44761 aaagacggtt tcatcatccg aagagaagca gttgaacatg gtattccatt gtttacttct
    44821 ttagacacag cagatgcgat cgttcgagtg atggaatccc gtgctttttc aactaaccct
    44881 atttaacggc gagcacatgt taagacaaaa agacgaaaaa ccgttgcagg aggggacaga
    44941 tgaaacaaga aatgatggaa atcgttcagc aaaaacagct ggcaccaaag atttttgaaa
    45001 tgacattgaa aggtgatcta gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc
    45061 tcgtgccgcg tgcagacttg ctattacgcc gaccgattag cttgaatcat atcgaccacg
    45121 aaacaaatac ttgccgtatc atttatcggg tggaaggtga ggggactaga gtcttctcta
    45181 ctctttctgc cggagaccat ttagatgtca tgggtccttt aggcaatggt tttgatttgt
    45241 ccaatcttca tacaggagac gaagtattta taatcggtgg aggaatcggt gtccctcctc
    45301 tatatgagtt atcaaaacag ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt
    45361 ttgccacaca tgaagtcgtt tattatgaaa aagaatttaa tcgtctagga gaaacaagaa
    45421 tcgctactga cgatggaaca tacggtatgc acggaaatgt cggtaattta ttgttaggaa
    45481 cgaagacgga acctacagct gtttttgcat gtggttcaaa cgggttattg aagacagtgg
    45541 aacagctgtt ttcttcgcat caaaatgtcc agttatcatt agaatcccgc atggcatgtg
    45601 ggataggggc ttgctatgct tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa
    45661 gtgtgaaagt ttgtgacgaa ggaccgattt ttaaaatagg agaggtaatt atatgaatga
    45721 gaatccttta gcggtcaaac ttcctggcct agaattaaaa aatcctatca tgccggcaag
    45781 cggctgtttc ggttttggca aagaatacgg aaaatactat gatttgaatc agcttggatc
    45841 gatcatggtc aaggcaacga ctccaaatgc ccgctttggc aatcccacgc caagagtggc
    45901 ggaaacacca tctggcatgt tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt
    45961 tatgcataaa cttcttccag aattagagga gtacaatgag ttacccatca tcgcaaatgt
    46021 tgctggtgct tgtgaagaag actatgtaga agtttgtagc aaaattggcg aagcaccaaa
    46081 tgtgaaagca atcgagctga acatttcttg cccaaatgtc aaacatggcg gaattgcttt
    46141 tggaacagat tctgatgttg cttttcaatt aacacaagca gtaaagaaag tttcttctgt
    46201 tcctgtctat gtcaaattgt ccccaaatgt aacagacatc gttccaatcg ctcaggcaat
    46261 cgaagctgga ggggcagatg ggtttacgat gatcaatact cttcttggta tgcggattga
    46321 ccttaagaca agaaaaccga ttttagccaa tcaaacaggt ggactctccg gaccagcaat
    46381 caagcctgtc gctattcgtt tgattcacca agtagcaagt atatccagcc ttccaatcat
    46441 tgggatgggc ggcgtgcaga cagtggatga tgttcttgag atgtttatgg ctggagcaag
    46501 tgctgtagct atagggaccg cgaatttcac cgatccgtat atctgtccta aactaattaa
    46561 agagctccct gtgcgaatga gcgaactagg catcgaatcg ctggaaagat taatcaaaga
    46621 agtgagagag gaacgtgaac gatgaatcaa ctagaccaac gaccaattat tgcattagat
    46681 ttttcaacta ggcaagaggt agaagacttt cttcgatttt tcccgaaaga agaaaaattg
    46741 tttgtgaaaa tcggaatgga attgttctac caagaaggac ctgaaatcgt acgttatcta
    46801 aaagaggctg gtcataatgt atttttagat ctgaaacttc acgatattcc gaatacggtc
    46861 gaaaaagcaa tgcggggatt ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct
    46921 ggcggtattc gtatgatgga ggcggcaatg cgaggattag aagaggggac tccagaaggt
    46981 ggaaaacgtc cgctacttct agctatcact cagcttactt ctacaagtga agaagaaatg
    47041 catgccgatc aactaatcga agtttcttta gaaaaaagtg tgatccatta tgctagctgt
    47101 gccaaaaaag caggattaga tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa
    47161 gaaatggcag gagacgaatt tgtttgctta actccgggta taagaccaga aggaacagtc
    47221 gcaggagatc agacaagagt agtcacacct tctcaggcaa aaaagatcgg ctcaactttt
    47281 atcgttatag gtcgaccaat cactcagtca actaatcctt ataaagcata tcaaacgatt
    47341 caaacagaat ggagctaacc taaaatgaat gttgaacaaa gtatcgcaaa agacttatta
    47401 gaaattgaag cagtattttt aaatccaagt aatcctttta catgggcaag tggaatcaaa
    47461 agcccaattt actgtgataa ccgaatcacg atgagctatc caaaagtccg aaaagagatc
    47521 gcaaaaggat tagcaagcaa aatcaaagaa gcttaccctg aagtacaagt gattgctgga
    47581 actgcaacag caggaatccc tcatgccgca tgggtagcgg aaatactgga tttgccaatg
    47641 gtctatattc gaagtaaggc gaaagatcat gggaaaggta accaaatcga aggacgtata
    47701 tttgaaggtc agaagatggt tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg
    47761 gaagcagctg aagcagcaaa aagagaaggt gcggatatcc ttggtgttgc ggctatcttt
    47821 acgtatgagt tgccaaaagg aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg
    47881 ttgaccaatt attctgtatt gattgaagca gctttagaag atcgttatat cgatgagcaa
    47941 gaattaactc tgctaaaaga atggaaaaaa gatcctgaaa attggcagat ttaagcgcgc
    48001 ttgatgaaga tagagaaatc cgatttgtgt ctccagaatc atcaattgga atagcaaaaa
    48061 tccaaaaact gattgcgaga agcatctttt catcaggtat gaaaagaaac ttctatcgtg
    48121 atcagttttt gattttttta aaattcattt ttctaagact ggttagcaga aagaagagaa
    48181 taatctcttc tcttttttct attatatata gattttctct attttttggt aagggagctg
    48241 tttcaaaaat ggtaaagagc ggaaatgttg cgaatatcag ttatttaatg atatgatatt
    48301 cggaaaaaga aatctcaaag ggaggtaact tttatggggt tgaatgaatt tattttagga
    48361 ttgaacaatt tagagacgat cacaagggca cctggatttt ttaaatatac agagcataca
    48421 gttgcagcac attcgtatcg tgtagcatct attgcgcaag ttctaggaga cattgaagaa
    48481 gcgaatggac aaccgatcga ttggaaactg ctctatgaaa aatcattgaa tcatgactac
    48541 acagagcgtt ttattggtga tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga
    48601 gggatgctgc aaacagttga agaaaagatg acggacgaat tcatcaacca agagattcca
    48661 gctgagttcc aagaagttta tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa
    48721 ggagaactat tagccatttc agataaggtt gaccttcttt atgaatcttt tgatgaaatc
    48781 atcaaaaata acccggaaaa agtttataag gagatgttca ttgaggcagt gactacgatt
    48841 aaaagctttg aacatcggcc ttctgttcag tatttcttta aagagatatt tccggaacta
    48901 ttgaaccagg aattttatgg aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag
    48961 aaagaagaaa aataaaaatg atatgagatg cagggggaag tatagttgaa aaagaatatg
    49021 gatgtcgaat ggggctattc tggagacaaa ggtccggaaa attgggcgag tttatgtgat
    49081 tggtttgcga gaggtgcgga atttccattg cagtcgccta ttcatttgac caagggggaa
    49141 gaaaccaaaa tagaaggaga cacgctttcc tttcattatc aaaagcaacg gtttactgac
    49201 aaggaattca aaaacacgat tcatttagta ccttttgacc aactcagtta tgtagagttc
    49261 aagaaagaac gctattattt gacagatatt catttccact tgccttctga acatatcatc
    49321 aacggaaaac aggaagatat tgaatttcat tttgttcata tgaacagcaa aaaagagaat
    49381 ctagttgtgg gtgtcatgta ccagctaatg gtacaggaag gttggctgta taatcaagaa
    49441 aatggggaaa gttggaactt agaaaaacat gagcattggg taaatccgga tgtgttcttt
    49501 ccggagcaaa aaagccattt ccattatatt ggctcattga caactccgcc taccagcggg
    49561 ccaatccaat ggtttgtaat ggatcatgta ggaaaactga accaagagtt tcttctcccc
    49621 ttcgatggtc agtatgctcg accgaacaac cgaccaatcc agcccttgaa tggaagagaa
    49681 atttattatt tcgaggagtt tgagcaatga atattcagca gatgaaatac gtggtcgctg
    49741 tagcaaacaa tggtagtttc agagaagcgg caaagaaaat gtttgtctcc cagccaagtc
    49801 tttcagctgg tatcaaagaa ttagaaaccg agttagggat cacgctgttt actcgaacga
    49861 atcgaggggc atatttaaca gaagaagggc aagacttctt aaatcgtgcc gagaaaatat
    49921 tggtccagct tgatacatta gaaactcact atctaacgag tgaaaagaga gacaattttt
    49981 cgatcgctgc ccagcattat gatttcttag gtcctttgac cgcacagcta ctcgaggaat
    50041 tcaaagacga gatcaaacaa tttcggatca tggagacgac aacagcgaga gtgatcgaag
    50101 aagtcaaaga gcagcacagt gagatcggca ttatttatat gaatgagcat aatcaagctg
    50161 gtatcagaag atatttacaa caaggagagc taagtggccg cagcattgga aaattcaaaa
    50221 cgcatatttt tttaaggcaa ggacatccat tagctgaaca cgcagagatc accaaagaag
    50281 aattgttatc ttatcctcaa gttcgtttta cacaggatgg aaataatttc ccgtattttt
    50341 atgaagacct tatcgagatc cctgatcaag aaacagtgat ttatacgagt gatcgaggta
    50401 ctttgatgaa tatcgttctt gaaactgatg cttacgcttc aggatctggt attgtgattg
    50461 gagggattcg cgagcacttg cggttaatcc ctttggcaga aggcgaacta aatgaatttt
    50521 atatcattta ttcggcgaaa cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc
    50581 ttactcggtt actggaccag cagagctaag aaaagcagat gagaacgctg tttattgaac
    50641 accgttgatc aaaaaagagg ggttgtgaca gaattttgtc acaactcctc tttttgtagc
    50701 aaacaattta taactaattg ctgttgtggg aaagtacgca aatatgattc tagaaacact
    50761 cattgattac ctgttttgtt tcatagctgt aattttttct tcttctggtg tcacgataat
    50821 cgtcttttgg ttttcataaa taacataacc aggcttggcg ccatttggtt ttcggatatg
    50881 ctttacctgt acaagatcaa ccggcacttg ggcagagtat cggtattttg aaaaataggc
    50941 agctaactcg gcagcttctg taattgtttc atctgagggc ttgtcacttt taatgatgac
    51001 gtgcgaaccg ggtatatttt ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat
    51061 tgtcagctgg tcattttgaa gattgttttt accaacaaga atgagcgttc cgtcagaaga
    51121 aaagtattga tcaggctgag atggtttctt acgtttttgt ttttttaagt tcttcttttt
    51181 caggtatcct tctgaagtca attcttcttt aattgcttcg atatccattg ggccggcaat
    51241 ttccagttga gataagacag attcaagata ttggatttca tctttggctt cttggatttg
    51301 ttcgccaatc agcttaacag cgttcttcag cttttggtat cgatggaaat atttctgtgc
    51361 attttgattt ggtgtcaatg ccggatctag tgctattttg attggccgat cttcttcata
    51421 ataattaggt aaaacaactt cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag
    51481 tagttcgcca tttctacggt aattctcagc gttttcggat tctttgagtg tttgttcacg
    51541 ttttttcaat ttatttttat ttcgttttag ttcattttcg atttttcgaa tcagttctcc
    51601 gccttgttgt tttgcccgat ctttctctgc tttttcgtga taatagctat ctaataattg
    51661 actgagtgtt ggataagctg ttacagcaga tgcctgttcg gacaatacct gataagcaat
    51721 cggtgtgaaa aaatctttat tttccgtttc gtaaaaagta ggaataggct gatggctgat
    51781 ggctgagaaa aattcgtccc atacaaccat cttttcatta ggacgttcca gtaatctttt
    51841 ggtcagctct tgagcgatat cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg
    51901 atctagttca gtttgggaaa gccgctgaaa gattttttct ttctcggagc taaatgggtt
    51961 caagacctct tgttttggcg gagcaacata ttctacacca ggaagcaaag aacgataagt
    52021 gttttgagag cttccaatgt gtttgatcgc atccagtatt tttccagttt ctcgattaac
    52081 taagacgatc gtactatgac gtcccattaa ttcaacgatc aatatgatat tttgtagatc
    52141 acccaactcg tttcgtttag caaagtggaa atggatcact cgatcatttt cgatttgttc
    52201 gatgtcttct aaaatagcgc catccaaata ttttcgaagc atcatcacga agttaggcgg
    52261 attatcagga ttctgataat cgatttgggt aatctgtaca cgagcataac ttgggtgcgc
    52321 agataataat aatcgatggt ttttgccgcg tgaacgaatc accagcacga tttcattttc
    52381 ataaggctga tggattttcg agatccggcc agatagtaac gtttcacgca attcgttgac
    52441 catcgcgtgg gtaaatacac catcaaagga catggttcat tcctcatttc ttttcataag
    52501 tatattcatt ataacgaaaa aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag
    52561 tctagaaaaa tcttggacta attacataaa aaacagtgtt tctatcgttg aaatccaaat
    52621 ttttaggtat aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa
    52681 gtagttaaat cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat
    52741 ccaagtaagt attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt
    52801 gcaatgtttt tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg
    52861 tccataccaa ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa
    52921 caaagccggg cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg
    52981 cttgatgttt ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat
    53041 ccgatcgttt atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg
    53101 acatttattt tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga
    53161 ataactgatt ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc
    53221 aaacaattag aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct
    53281 aattgagaca ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga
    53341 acaagtgatg tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa
    53401 ttcaaaaatt cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt
    53461 atcaaaacgt atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt
    53521 attaatggtg agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat
    53581 ctatgctaga catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta
    53641 aagacaatga ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata
    53701 ataaataaaa agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag
    53761 ttgtagtaat tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg
    53821 aaaaagaaag tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat
    53881 acgtgagcca tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag
    53941 aaggattaaa agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga
    54001 gcaagctagc cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa
    54061 tcgcaacacc tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag
    54121 gtgctgtaac ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta
    54181 atgtaacagg tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt
    54241 tgttgccaaa tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat
    54301 accaagtagc acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg
    54361 cggtgccttc cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt
    54421 ttatctatgt tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag
    54481 caaacaaagc aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac
    54541 tagcaacaat cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag
    54601 aacttgcgag tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg
    54661 atacagtgat caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga
    54721 aggaaaaagc caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca
    54781 aatgatcgta tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt
    54841 catgacttat cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg
    54901 tgctgctgtc tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt
    54961 aggcgtaggt gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa
    55021 gataccggtt attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt
    55081 tgtgatgcga acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca
    55141 tcgctttggg ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat
    55201 cttgattgct gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac
    55261 aggagacaac gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt
    55321 aggtttaact ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga
    55381 cggatatgca gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat
    55441 catcggtgaa gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt
    55501 cggcagtatc atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac
    55561 ttatctgaag ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa
    55621 gaaagcactg aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg
    55681 acaatcaaaa atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg
    55741 gatgaaatta accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt
    55801 gctggaaaaa gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg
    55861 atctctttta aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt
    55921 tctcgagttt tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat
    55981 ttgctacttg cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat
    56041 caaaaagaag attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg
    56101 gatactccag cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg
    56161 acgatcacag ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa
    56221 acagctaaag cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt
    56281 ctcatgatca cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc
    56341 caaaaaggga aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc
    56401 gaccttttgc agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag
    56461 tagaaggact tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata
    56521 aacaattaaa catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa
    56581 atagaatatt ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc
    56641 tagacgtttc gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac
    56701 aaaggaggaa taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg
    56761 cttctcacag tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg
    56821 cggtgtattt agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc
    56881 gaacttttga tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt
    56941 atctggatga ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg
    57001 aaaagggtcg tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag
    57061 aagctgggat ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa
    57121 aggcagtcac tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa
    57181 acaaccaaac attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt
    57241 gtaagataaa agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa
    57301 gctgtcttct agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc
    57361 tcttagatat aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta
    57421 gagaaacacg tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg
    57481 gcatgttgat ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga
    57541 tccatgtgat tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct
    57601 cgcataaacg gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt
    57661 tttattaaat tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta
    57721 tcagtaagaa acgaggagga accacaatga gtcggatgga tcgctataag gacatccata
    57781 agaaggcgaa acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac
    57841 gaacgcgtat ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat
    57901 ttgaacattt gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac
    57961 aacaaaaaaa taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa
    58021 gaaagcgatc gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag
    58081 gtatctcatt ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga
    58141 cttcagaaac ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg
    58201 gaagtgatac acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg
    58261 atggtccttc ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc
    58321 caggggtagg acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc
    58381 gtcaaacttt agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca
    58441 aatcgtttga aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa
    58501 aagatctgaa tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg
    58561 ttcttttgca gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac
    58621 gccaacagca agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc
    58681 gtgcacctta tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt
    58741 tcttgacgaa aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg
    58801 tgccagtaga cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa
    58861 ttgataatca aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa
    58921 gatcaagaag aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa
    58981 aaacgctttc ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct
    59041 gaacttcaaa aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga
    59101 agcatcaggt atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga
    59161 agaagcaaaa aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt
    59221 tgcagatacg ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt
    59281 catccagaca agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg
    59341 taagattgag atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga
    59401 tcgatttggc atcaactggc agattaacca agagtgacag ctaaagatga atattttctg
    59461 gaaacatcaa gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg
    59521 actatacggt atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc
    59581 cgcaaaaaat taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct
    59641 tcacaagcaa aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat
    59701 gaaatcagcg atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg
    59761 gtagatatgg ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat
    59821 ttgcctattt tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt
    59881 tcgatcgtaa aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat
    59941 cgagaaggtg aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa
    60001 aagaactatt tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt
    60061 aagaatctcc aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag
    60121 aacgctgatt ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga
    60181 aaagggattg atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt
    60241 tatcagctgc aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt
    60301 ttaattacga caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa
    60361 acccaagaag agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac
    60421 ggacggattc ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg
    60481 ctatctagtt tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt
    60541 gatgctgctc aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt
    60601 tacattacag aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt
    60661 ttaggaatag aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg
    60721 aaaaaagttc aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc
    60781 tttgcgaagc tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg
    60841 gctatgtttg ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa
    60901 ttaaagctaa aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc
    60961 atcaagtcaa aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca
    61021 gaagtagaac tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga
    61081 acgttgatca cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa
    61141 attctcaaag aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat
    61201 cttaagcaga agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga
    61261 atcactcttt gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg
    61321 cgctgggaag ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta
    61381 gagaatatca aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag
    61441 gtagactttt cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac
    61501 gatcaattag gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc
    61561 gctacatgct tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag
    61621 ctgactaaaa cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa
    61681 ggttttactg gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca
    61741 atcggttttt cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa
    61801 gaaacatcaa gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg
    61861 aatatttatt ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat
    61921 caagcaaaaa cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga
    61981 aaaaagtgca ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc
    62041 aagcatttct aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa
    62101 cagcatattc accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt
    62161 atttttttca taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg
    62221 tggtggtagc ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc
    62281 aatattttgg ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt
    62341 tgtcattttt tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac
    62401 ttggatataa tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa
    62461 aaatgaaaaa gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag
    62521 aagtaaaaga attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg
    62581 atggttctgt acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt
    62641 cgatcatgaa tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc
    62701 gactaaagaa attatcaaaa tcaacgcgaa agttatttta ccgtcactcg ccaggcgaaa
    62761 tgatcggtgt aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag
    62821 tattcaatca agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt
    62881 tagctaaaaa acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt
    62941 tgtttcttca attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta
    63001 tcataatgat taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt
    63061 atttacgaca aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag
    63121 catgtcatta aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat
    63181 aaaaatatgg aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag
    63241 tgaatagtag gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta
    63301 cataatatat attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt
    63361 tatcaaaaat cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg
    63421 cagtttttct tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag
    63481 ttaatcaaat atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt
    63541 ttaattaacc acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt
    63601 tcatatgttc atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat
    63661 ttgcttacct attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag
    63721 ttttaggatt tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt
    63781 aaagcacttg ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca
    63841 acttcacgaa caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg
    63901 tcgtattcac cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata
    63961 gcttttacga tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca
    64021 atgatctcat aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca
    64081 atacgatggt ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac
    64141 catgcagcaa cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg
    64201 tcaactttta atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca
    64261 gtaccgataa cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata
    64321 tctactgggt ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt
    64381 acgatttggc gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt
    64441 ttttggttga taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca
    64501 cctgaccata cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct
    64561 tccccttctg ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg
    64621 ttgatcattg cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta
    64681 cgacttgttt ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa
    64741 aagtatagtc ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat
    64801 ttttgatgga aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta
    64861 tatcccctga taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg
    64921 caacgatata ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga
    64981 aagattttct tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt
    65041 gaataatttg aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa
    65101 cgaaaaactc gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag
    65161 ttcttgaaag gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta
    65221 gccaaaatga gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc
    65281 taatactcta agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc
    65341 taataaatga gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt
    65401 atgactgtat gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact
    65461 tgtttttccg ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt
    65521 tatcaaaaaa gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga
    65581 taaggtttct tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat
    65641 tctttgagta gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc
    65701 tccattaata atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg
    65761 tgtatgctcg ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg
    65821 aaatagatcc cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc
    65881 attgatattc attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa
    65941 aaatgtctaa ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc
    66001 ttattagttc acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt
    66061 tctatggttg ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac
    66121 tatatttttg ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat
    66181 gaaattttct ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc
    66241 aattcccagt atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac
    66301 gactgctccc tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa
    66361 aatcggacaa aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct
    66421 ccccctcttc tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg
    66481 ctttggattg atcaattctt tggcagtcag catgattgct gccttttatc acggagatga
    66541 acgagcctct atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac
    66601 atttgttgca ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat
    66661 cagcttacct attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact
    66721 tcaatcgcat ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag
    66781 ttatggtatt tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc
    66841 cacagtcctg acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat
    66901 catgcaattg gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca
    66961 tcgctatatg cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac
    67021 tgtgataaac ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt
    67081 attcattcct tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt
    67141 caatacttca atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt
    67201 gctctttctc tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag
    67261 tatcttgctg ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa
    67321 gacagtgata agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg
    67381 gaggtcgtgt aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga
    67441 ctttgagatc cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg
    67501 gccagcgtac tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt
    67561 tcttgatcaa ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa
    67621 tggacaaaaa catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg
    67681 tatctttgta ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa
    67741 atcagatatt ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc
    67801 gctttatgtc gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca
    67861 tccagtcact gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa
    67921 tggctgtgta gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg
    67981 agagtccgat gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac
    68041 aagaaaaatg aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac
    68101 tggaatggtt caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt
    68161 ttcaagcgat tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt
    68221 atgtagtgat ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga
    68281 tgcgtatcaa tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg
    68341 aacgatatta gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga
    68401 ctattcgatc ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga
    68461 atttttacgc atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac
    68521 gaatgaatta gggtttattt atgggagagc aaaagattgg atatttattg attggtctga
    68581 aatggataaa gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc
    68641 catgatcact tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac
    68701 tcatctctat caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga
    68761 tgaagaactt ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca
    68821 tgcgaatatt ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt
    68881 aaagaatgtc ttattgaatg acactatcac acagattact actccatatt tcaaattcta
    68941 tgaacaagat gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga
    69001 ttattgggga gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac
    69061 tcaaacagga aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca
    69121 tgcatgggga gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac
    69181 aaagccaggt tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa
    69241 ttgtacgcta cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt
    69301 gattacttct gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct
    69361 agcaaaacag ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact
    69421 gcttgcgaca tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt
    69481 ttgaagtttg cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga
    69541 aagctagata atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga
    69601 ccgtattctc ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat
    69661 gtagaagaag cgattttaag atctcctaat actggaagga taaataatgg cagccaacta
    69721 atcaataagc tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg
    69781 ccgaagacac cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact
    69841 gtagtttgca agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa
    69901 aagccaatta atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt
    69961 ttagaatcgg gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac
    70021 accattcgaa ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc
    70081 agtgcaaaga ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca
    70141 gctaacaaag caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa
    70201 tctcgcaaaa agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt
    70261 ccaaaaaact ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac
    70321 gcaacttgat tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata
    70381 gttaatagtg ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac
    70441 cccgcgctta ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta
    70501 agaaaaatga atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact
    70561 agcatgatca aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca
    70621 ttgctaggaa ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt
    70681 agtgaattac tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg
    70741 atagttcctt ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca
    70801 atcaaagaaa cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta
    70861 tccattgata gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt
    70921 ttcttttact tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat
    70981 agaagttagc gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga
    71041 tacacttcca acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc
    71101 aagtgcttgt ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac
    71161 tgcaaaaatt ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg
    71221 ttgatgcgtg taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg
    71281 ccaaagcccg aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg
    71341 gatgggctgg attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta
    71401 cttcgtttac gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga
    71461 taaacgcacc atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt
    71521 tattttctga taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt
    71581 gatccgataa ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct
    71641 ccttcttttc taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt
    71701 ctacgccttc ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag
    71761 gcattgaaaa tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt
    71821 ccaagtcagt catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga
    71881 gaggtacagt agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag
    71941 ctgaattagt ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga
    72001 tcaatgaaga gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt
    72061 cttctgcgtc cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt
    72121 cttcagggat gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt
    72181 cgtagatgga agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag
    72241 aataggaaat acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac
    72301 gaaacgaagc aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac
    72361 attccagcaa aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc
    72421 aaagagagaa aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata
    72481 cttgtcgact aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt
    72541 ccatggatgt ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca
    72601 ttcgatttgg tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa
    72661 ctttggcgta ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag
    72721 caagtgactg catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg
    72781 accctagcag cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta
    72841 attcatttct tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat
    72901 cttgaaaatg aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat
    72961 ctcttcgctc ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg
    73021 cccagtcgat tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa
    73081 aaggagttaa ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt
    73141 ctagactgat ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt
    73201 tcacctccta cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa
    73261 cttacagttg gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa
    73321 aaaagcaggt gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga
    73381 tcatgtttat tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt
    73441 tcgatatctt tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt
    73501 gaaccagata aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat
    73561 tcttctttgt cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt
    73621 ttaggggcca ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg
    73681 gttcctccta gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa
    73741 acaaacccga ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat
    73801 tccttcttcc aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc
    73861 gccattaact aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga
    73921 aattactttt tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg
    73981 ttcgggcaaa tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc
    74041 ttcttgtcga tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact
    74101 tggacgttct ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa
    74161 aaaatccaga tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata
    74221 gctgatatca tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg
    74281 tccatataaa tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct
    74341 gaaataatca taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac
    74401 tttttggact gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag
    74461 acctttttct tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg
    74521 tttctctact agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa
    74581 ttgactaggt gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag
    74641 ctgatcagta tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat
    74701 ttttttgata ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt
    74761 actctttcca ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg
    74821 ttttcagtat atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag
    74881 ttcagaaaaa cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa
    74941 aatcaaaaag atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga
    75001 tcatgcttat tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga
    75061 ttgtcgatta catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag
    75121 caaaagctac gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca
    75181 gcggatttaa agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt
    75241 tcaatcaaac gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg
    75301 caaagaaaat ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac
    75361 atgaagattt agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg
    75421 caaaaggtga ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata
    75481 agtttttgat catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta
    75541 gcgaggattg cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa
    75601 tgacctattt gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag
    75661 aaatggccaa acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta
    75721 aagtagcgac tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag
    75781 tgatatattc tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga
    75841 tgcaaaaagg ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat
    75901 aagaaagata gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt
    75961 ctttttcaaa cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat
    76021 gaggaatagc taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag
    76081 gaggaaaatc agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga
    76141 tactttctgt acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac
    76201 aagtaagcga tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg
    76261 ccctttttcg attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac
    76321 ttgataagcg agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt
    76381 cggtaagtct gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg
    76441 tgtattttct ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag
    76501 cagcacagta ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa
    76561 aataccgatc gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt
    76621 agtaaaggga tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt
    76681 catgttttct tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata
    76741 atgagagatg atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc
    76801 tagtatccct attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt
    76861 ctttggtttc atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca
    76921 tttgcgtaat ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct
    76981 tgcgtctagc aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca
    77041 catagattcc cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt
    77101 caggagaaat caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac
    77161 ggtttgttaa tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat
    77221 attttagacc tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt
    77281 ctttcgccga tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg
    77341 tgtcactatc ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa
    77401 cgtcaccatc tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg
    77461 tttgatcatt tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat
    77521 ctgctttttc gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg
    77581 aaggaatata attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat
    77641 aatttactgg gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag
    77701 aatcattatc aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact
    77761 tgttttgcgt tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt
    77821 gaattttttc accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt
    77881 tagacgtatc atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa
    77941 aaggcgtgat tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta
    78001 aattatattt tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct
    78061 cggcaaagtc attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg
    78121 tttgatcagt tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc
    78181 ttggtgttcc ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg
    78241 cagtaccgat ttttgtaact ttcaacgttc catcattacc gactgtattt ttagggtaga
    78301 gatggatagt atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca
    78361 tctcatatac agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt
    78421 cttctttgat cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga
    78481 catttccatc agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga
    78541 ctgcttgttt tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag
    78601 tgacgttata aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca
    78661 tttctttccc gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa
    78721 ggatgacttg agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga
    78781 gtaatggtaa aataaggaaa aggactccta acataacgtt tattttttta tgatttttca
    78841 ttttttcact cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat
    78901 accaacaacg cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct
    78961 attttctttt ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa
    79021 agttgaaatg gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat
    79081 ctcttttaat cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc
    79141 ggaaatcagg tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg
    79201 ctggaggtaa tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc
    79261 tggtttattt gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt
    79321 tgtactgaca ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg
    79381 tgctgtactg cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc
    79441 caacgaaggg atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc
    79501 ttctgtcagc ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc
    79561 ttcatccgca ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt
    79621 cgtatgcaag gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa
    79681 taccttctca aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat
    79741 agttgaaagt ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc
    79801 cttttctttg acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt
    79861 ttcttgatct ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt
    79921 agttgttgct tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg
    79981 tgcttcttct accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata
    80041 aacttggaat gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc
    80101 gtcatttgga tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat
    80161 ctgaaccatt tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa
    80221 caagaagaga cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct
    80281 tttttcaaaa aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt
    80341 cctaaacggc caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct
    80401 aaagaaatct ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc
    80461 acttgaattg acccatcttc gtgtataact atagtaactg gctcttttag accttggtat
    80521 ccttctggtg taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga
    80581 aaggtatctg tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt
    80641 ctgaattttg ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca
    80701 aacgctttta gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt
    80761 tttcccgtgt aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa
    80821 gtatcatatc cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa
    80881 tcaaaactgg caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca
    80941 tttgcttctt ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc
    81001 ccttgcatcg catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc
    81061 aactggatcg tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca
    81121 gaagcgatct tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt
    81181 gtttctgtca gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag
    81241 gttccgtcgc catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca
    81301 gcgccaataa gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt
    81361 gtgaattgtt tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt
    81421 acaggtaatt ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt
    81481 agtgataata tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca
    81541 cgttcccacg tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc
    81601 caagctgcag catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga
    81661 tcagctagat tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga
    81721 gctttagcag atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa
    81781 gttgtcctgc cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg
    81841 attctcactt ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc
    81901 ttgatggtat ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca
    81961 ctcttgactg aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt
    82021 tgtccattgc ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata
    82081 tctgttgcat gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt
    82141 tgtcgcatac gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt
    82201 tggatgccaa gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg
    82261 gttgcgataa acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag
    82321 attcttgaga ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg
    82381 taataattgc tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca
    82441 ccatccgtca gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct
    82501 gcatctctta gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat
    82561 tttacttgat ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta
    82621 tatccttcgc ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct
    82681 gctaaagtat cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg
    82741 ttcatacttc ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt
    82801 acatttccac gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca
    82861 tactttcgta tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg
    82921 gtccgatcat taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt
    82981 ttattgcctt gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga
    83041 taagtgccct gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc
    83101 acagctggca aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt
    83161 gatgattctt cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt
    83221 tctgtctcat caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg
    83281 tggtaaagtg ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag
    83341 atttccaacg taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt
    83401 tccacatcac ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg
    83461 gtgttattcg catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact
    83521 tcagctgaaa ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat
    83581 ggcacaaagt ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct
    83641 gtggtcgtca tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat
    83701 tattaaaata aacaggcgtt acataatttt atttatttta atatgataat tattatacga
    83761 agggcgctaa agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat
    83821 tccttcgatt aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc
    83881 aggttgttgg aaggaaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct
    83941 gacaagtgga tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt
    84001 gtaaggtata tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca
    84061 caaaaattgg ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg
    84121 gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt
    84181 aaaacgattg agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag
    84241 ttgaaacaat gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt
    84301 tttcagttaa ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt
    84361 ttaagttact acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa
    84421 attatggtca agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa
    84481 tctttcttta ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt
    84541 gaatggaaag ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta
    84601 atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat
    84661 tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag
    84721 atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa
    84781 gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat
    84841 acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata
    84901 cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat
    84961 ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac
    85021 cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa
    85081 tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact
    85141 gctattactg aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg
    85201 aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc
    85261 gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt
    85321 ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat
    85381 tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca
    85441 attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa
    85501 actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca
    85561 tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt
    85621 tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca
    85681 ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt
    85741 taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc
    85801 caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc
    85861 tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat
    85921 tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc
    85981 tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt
    86041 tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca
    86101 tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta
    86161 gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta
    86221 agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac
    86281 cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg
    86341 aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt
    86401 cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa
    86461 agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta
    86521 tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg
    86581 aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt
    86641 tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta
    86701 atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt
    86761 acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa
    86821 ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata
    86881 agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa
    86941 agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt
    87001 cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa
    87061 aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat
    87121 gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc
    87181 tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg
    87241 tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta
    87301 ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat
    87361 cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt
    87421 tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc
    87481 gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg
    87541 tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa
    87601 tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt
    87661 tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat
    87721 caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat
    87781 agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt
    87841 tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa
    87901 attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc
    87961 ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta
    88021 aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg
    88081 caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg
    88141 gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt
    88201 tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata
    88261 gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt
    88321 tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct
    88381 ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct
    88441 tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt
    88501 tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg
    88561 attctgatac gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc
    88621 gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa
    88681 gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca
    88741 aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt acagatatta
    88801 aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt tgattctcga
    88861 acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa
    88921 acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt
    88981 aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt
    89041 gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa
    89101 aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa
    89161 aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa
    89221 atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg
    89281 attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt
    89341 tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta
    89401 acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa
    89461 gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg
    89521 gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc
    89581 aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat
    89641 ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa
    89701 atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat
    89761 aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact
    89821 ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat
    89881 caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta
    89941 atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt
    90001 aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac
    90061 aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt
    90121 cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg
    90181 cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt
    90241 cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc
    90301 caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga
    90361 tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg
    90421 gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt
    90481 tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta
    90541 atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt
    90601 cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg
    90661 gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg
    90721 gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat
    90781 cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga
    90841 caaattttcc atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag
    90901 ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac
    90961 ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt
    91021 caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat
    91081 acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc
    91141 cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt
    91201 ggtatattgt tacttataaa tttattctat aggagacgat tgttttctac aaatatttag
    91261 attggaattt gattaaaaca atttaattga aatttgaaaa catttatgtt aatgaaagtc
    91321 atattctaat aaaatgtcat ttgtttctat atgaattttt atgaaaaatt tcatataatt
    91381 tccactcgtt gtgatggttt aatttatcca atccatcaat aagcaaacaa cttatataaa
    91441 aaaggattaa ttctcaatat aattgctcct accatagaat aaatcttcat gtaaagtaat
    91501 gaagtaccaa ctatttctaa tacttcttta agaattatcc caacaatatc gttagacaaa
    91561 ccattattga ataaatggag agccaatcga ttctgaaaaa atgttttcta atttttcgga
    91621 aataaaagaa agagcctcct gccattagaa cgaaccaaat ccatttcatt gctcatgaag
    91681 taaccacata gctaaaaaga ggataaatca attaaaagat caaaagaatg actgcactaa
    91741 taaaataact aaaaatgtat cttcttactt catatctttt agctagtact tagttaaaaa
    91801 tttgcttcct aagatcaaga caaactgtct atcttcattt gatgaaatgc ggtaaaatat
    91861 agaaaatgaa cactattgga gggaatttgt aatggacagc aaaactagaa aacaactgca
    91921 agaaaagtta aagcaacgta cacacttgct cccttccgtc tttcaacagt ttttagaaga
    91981 aaatcgttct tccctttcat taggcagtcg ctatgaatac gcaaaagatt ttcatctgtt
    92041 tactgattat ttgagaaaag tattttctga agaagtggat gatcagctga ttatccattt
    92101 agaaaaacaa gattatcaga atttcttagc taaaatctat cgacatactc gttcgttcca
    92161 aactacagcg aaccaagaaa cggagcaatt gtttgaaaat agctattacg ggatttcaag
    92221 gaagcaatat tcattaaatc attttttacg atttttgtat caaaaaggac tgattgaaga
    92281 agagatttct cttttgggta caagtttacc ggaaacaacc aaagcagctc cgcgatatat
    92341 cgatgcagaa cttttcaaag attttgagca tttgtttatc ccgattgaag gctttcaaac
    92401 aagcagagaa gctagctttg aagaaaagaa tagaccgagg aaccttgcaa ttactgccat
    92461 tcttctgtat tgcggattaa agatccatga agtagtagaa cttaaacgta aagatgtatc
    92521 tttgaccaag cagattctta atctgaatag aaaagaaaac agactgaata aagtaccaat
    92581 accaaaagaa gcgatgccat ttctggagaa ctattttaag ttaacagcac aaaatacgga
    92641 cgaaaattct tttgtttttc gttcctcaca tgatcagcaa atttctcctc ggactattcg
    92701 ccaaattttg agcaaaatta cagtatataa aaacgtatcg ccagaactat gcagaaaaac
    92761 atatcgttat tatgcagaac tatatttaga tgatgctgac gcggtaaatt atttatgtgg
    92821 taatcgatac ctctcatccc attcttttca agatatctgg gaaaagatgg ttgatttttc
    92881 ttatcacgaa ttagcggcat tcgttcgggt ttcttccata taaaacaaat gtatgatctt
    92941 tttttcacaa taaactatgt tattatataa aggattatgt tacgattatc ttgaatttgt
    93001 atattactta acaaaagata atggagtgtg aatgatggag accataacgt attccccttt
    93061 gaacttattt acgaattttc aagaagctgc agctaacttt tccgagaccc cgattatctt
    93121 tgatgagcca ctttcagctt tcccagaatt aggacttgaa acaacttaca aaaaatgctg
    93181 cgaaagtgtg ttgaaacggg cttatcaatt ggctcatctt ggagtgaaat ccggagacaa
    93241 agttattatt tataagagtt ccgcttttga cacatacctt ctagctgttt ctgtgtctta
    93301 ccttggtgca gtgcctgtca tgacttctta tcatctacca acaacaacaa tggaagtatt
    93361 tatcgatcgt ttagaagacc ctttcatttt atttgatgat gagacaaaaa agcgagtgag
    93421 agaaatttca aatggaacaa aatcaaaaca gattc
//
LOCUS       DAPBCS010000003        87830 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPBCS010000003 DAPBCS010000000
VERSION     DAPBCS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 87830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.003"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(191..760)
                     /gene="thiT"
                     /locus_tag="RD521_000290"
     CDS             complement(191..760)
                     /gene="thiT"
                     /locus_tag="RD521_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDT7770055.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(826..928)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(934..1905)
                     /locus_tag="RD521_000291"
     CDS             complement(934..1905)
                     /locus_tag="RD521_000291"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDT7770056.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(1999..3372)
                     /gene="glmU"
                     /locus_tag="RD521_000292"
     CDS             complement(1999..3372)
                     /gene="glmU"
                     /locus_tag="RD521_000292"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDT7770057.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(3875..4693)
                     /gene="purR"
                     /locus_tag="RD521_000293"
     CDS             complement(3875..4693)
                     /gene="purR"
                     /locus_tag="RD521_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDT7770058.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(4860..5684)
                     /locus_tag="RD521_000294"
     CDS             complement(4860..5684)
                     /locus_tag="RD521_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7770059.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(5657..6337)
                     /locus_tag="RD521_000295"
     CDS             complement(5657..6337)
                     /locus_tag="RD521_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7770060.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(6360..7298)
                     /locus_tag="RD521_000296"
     CDS             complement(6360..7298)
                     /locus_tag="RD521_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDT7770061.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(7543..8406)
                     /locus_tag="RD521_000297"
     CDS             complement(7543..8406)
                     /locus_tag="RD521_000297"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDT7770062.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(8548..11121)
                     /gene="mprF"
                     /locus_tag="RD521_000298"
     CDS             complement(8548..11121)
                     /gene="mprF"
                     /locus_tag="RD521_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDT7770063.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            11262..12068
                     /gene="proC"
                     /locus_tag="RD521_000299"
     CDS             11262..12068
                     /gene="proC"
                     /locus_tag="RD521_000299"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDT7770064.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(12147..13703)
                     /gene="rny"
                     /locus_tag="RD521_000300"
     CDS             complement(12147..13703)
                     /gene="rny"
                     /locus_tag="RD521_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDT7770065.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(14114..15163)
                     /gene="recA"
                     /locus_tag="RD521_000301"
     CDS             complement(14114..15163)
                     /gene="recA"
                     /locus_tag="RD521_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDT7770066.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(15272..16513)
                     /locus_tag="RD521_000302"
     CDS             complement(15272..16513)
                     /locus_tag="RD521_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDT7770067.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(16753..17331)
                     /gene="pgsA"
                     /locus_tag="RD521_000303"
     CDS             complement(16753..17331)
                     /gene="pgsA"
                     /locus_tag="RD521_000303"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDT7770068.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(17540..18463)
                     /locus_tag="RD521_000304"
     CDS             complement(17540..18463)
                     /locus_tag="RD521_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770069.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(19202..20488)
                     /locus_tag="RD521_000305"
     CDS             complement(19202..20488)
                     /locus_tag="RD521_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDT7770070.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(20481..21749)
                     /locus_tag="RD521_000306"
     CDS             complement(20481..21749)
                     /locus_tag="RD521_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDT7770071.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            22001..22462
                     /gene="mscL"
                     /locus_tag="RD521_000307"
     CDS             22001..22462
                     /gene="mscL"
                     /locus_tag="RD521_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDT7770072.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(22521..22967)
                     /locus_tag="RD521_000308"
     CDS             complement(22521..22967)
                     /locus_tag="RD521_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7770073.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(23060..23305)
                     /locus_tag="RD521_000309"
     CDS             complement(23060..23305)
                     /locus_tag="RD521_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDT7770074.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(23420..23998)
                     /locus_tag="RD521_000310"
     CDS             complement(23420..23998)
                     /locus_tag="RD521_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7770075.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(24075..24605)
                     /locus_tag="RD521_000311"
     CDS             complement(24075..24605)
                     /locus_tag="RD521_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDT7770076.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(24609..25469)
                     /gene="rlmB"
                     /locus_tag="RD521_000312"
     CDS             complement(24609..25469)
                     /gene="rlmB"
                     /locus_tag="RD521_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDT7770077.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(25462..25857)
                     /locus_tag="RD521_000313"
     CDS             complement(25462..25857)
                     /locus_tag="RD521_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDT7770078.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(25854..27266)
                     /locus_tag="RD521_000314"
     CDS             complement(25854..27266)
                     /locus_tag="RD521_000314"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDT7770079.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(27263..27799)
                     /gene="cysE"
                     /locus_tag="RD521_000315"
     CDS             complement(27263..27799)
                     /gene="cysE"
                     /locus_tag="RD521_000315"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDT7770080.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(27885..28124)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(28121..29581)
                     /locus_tag="RD521_000316"
     CDS             complement(28121..29581)
                     /locus_tag="RD521_000316"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDT7770081.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(29751..30908)
                     /locus_tag="RD521_000317"
     CDS             complement(29751..30908)
                     /locus_tag="RD521_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDT7770082.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(30942..32312)
                     /gene="radA"
                     /locus_tag="RD521_000318"
     CDS             complement(30942..32312)
                     /gene="radA"
                     /locus_tag="RD521_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDT7770083.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(32375..32902)
                     /locus_tag="RD521_000319"
     CDS             complement(32375..32902)
                     /locus_tag="RD521_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDT7770084.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            33132..33938
                     /gene="proB"
                     /locus_tag="RD521_000320"
     CDS             33132..33938
                     /gene="proB"
                     /locus_tag="RD521_000320"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDT7770085.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            33935..35182
                     /locus_tag="RD521_000321"
     CDS             33935..35182
                     /locus_tag="RD521_000321"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDT7770086.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            35258..35602
                     /locus_tag="RD521_000322"
     CDS             35258..35602
                     /locus_tag="RD521_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770087.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(35719..36045)
                     /locus_tag="RD521_000323"
     CDS             complement(35719..36045)
                     /locus_tag="RD521_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770088.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(36660..37862)
                     /locus_tag="RD521_000324"
     CDS             complement(36660..37862)
                     /locus_tag="RD521_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7770089.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            37979..38494
                     /locus_tag="RD521_000325"
     CDS             37979..38494
                     /locus_tag="RD521_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7770090.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(38693..39139)
                     /locus_tag="RD521_000326"
     CDS             complement(38693..39139)
                     /locus_tag="RD521_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7770091.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(39186..40664)
                     /gene="msr(C)"
                     /locus_tag="RD521_000327"
     CDS             complement(39186..40664)
                     /gene="msr(C)"
                     /locus_tag="RD521_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7770092.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(41018..41647)
                     /locus_tag="RD521_000328"
     CDS             complement(41018..41647)
                     /locus_tag="RD521_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7770093.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(41631..42095)
                     /locus_tag="RD521_000329"
     CDS             complement(41631..42095)
                     /locus_tag="RD521_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7770094.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(42582..43850)
                     /locus_tag="RD521_000330"
     CDS             complement(42582..43850)
                     /locus_tag="RD521_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7770095.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(44081..44818)
                     /locus_tag="RD521_000331"
     CDS             complement(44081..44818)
                     /locus_tag="RD521_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7770096.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(44885..45454)
                     /locus_tag="RD521_000332"
     CDS             complement(44885..45454)
                     /locus_tag="RD521_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7770097.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            45582..46454
                     /locus_tag="RD521_000333"
     CDS             45582..46454
                     /locus_tag="RD521_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7770098.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            46796..47668
                     /locus_tag="RD521_000334"
     CDS             46796..47668
                     /locus_tag="RD521_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7770099.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            47665..49623
                     /locus_tag="RD521_000335"
     CDS             47665..49623
                     /locus_tag="RD521_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7770100.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(49955..50806)
                     /locus_tag="RD521_000336"
     CDS             complement(49955..50806)
                     /locus_tag="RD521_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7770101.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            50929..51396
                     /locus_tag="RD521_000337"
     CDS             50929..51396
                     /locus_tag="RD521_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDT7770102.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(51659..52141)
                     /locus_tag="RD521_000338"
     CDS             complement(51659..52141)
                     /locus_tag="RD521_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDT7770103.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(52241..53104)
                     /locus_tag="RD521_000339"
     CDS             complement(52241..53104)
                     /locus_tag="RD521_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7770104.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            53702..54577
                     /locus_tag="RD521_000340"
     CDS             53702..54577
                     /locus_tag="RD521_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770105.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(54722..54874)
                     /locus_tag="RD521_000341"
                     /pseudo
     CDS             complement(54722..54874)
                     /locus_tag="RD521_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(55142..56473)
                     /locus_tag="RD521_000342"
     CDS             complement(55142..56473)
                     /locus_tag="RD521_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7770106.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(56622..57335)
                     /locus_tag="RD521_000343"
     CDS             complement(56622..57335)
                     /locus_tag="RD521_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7770107.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            57510..58934
                     /locus_tag="RD521_000344"
     CDS             57510..58934
                     /locus_tag="RD521_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7770108.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            59233..59940
                     /locus_tag="RD521_000345"
     CDS             59233..59940
                     /locus_tag="RD521_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7770109.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            59927..60250
                     /locus_tag="RD521_000346"
     CDS             59927..60250
                     /locus_tag="RD521_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7770110.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(60339..61376)
                     /locus_tag="RD521_000347"
     CDS             complement(60339..61376)
                     /locus_tag="RD521_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7770111.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(61697..62680)
                     /locus_tag="RD521_000348"
     CDS             complement(61697..62680)
                     /locus_tag="RD521_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDT7770112.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(63095..63865)
                     /locus_tag="RD521_000349"
     CDS             complement(63095..63865)
                     /locus_tag="RD521_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770113.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(63858..64793)
                     /locus_tag="RD521_000350"
     CDS             complement(63858..64793)
                     /locus_tag="RD521_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770114.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(65109..66758)
                     /locus_tag="RD521_000351"
     CDS             complement(65109..66758)
                     /locus_tag="RD521_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDT7770115.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(66875..69478)
                     /locus_tag="RD521_000352"
     CDS             complement(66875..69478)
                     /locus_tag="RD521_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDT7770116.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(69490..70539)
                     /locus_tag="RD521_000353"
     CDS             complement(69490..70539)
                     /locus_tag="RD521_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDT7770117.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(70565..70879)
                     /locus_tag="RD521_000354"
     CDS             complement(70565..70879)
                     /locus_tag="RD521_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDT7770118.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(70895..71341)
                     /locus_tag="RD521_000355"
     CDS             complement(70895..71341)
                     /locus_tag="RD521_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7770119.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(71342..73264)
                     /locus_tag="RD521_000356"
     CDS             complement(71342..73264)
                     /locus_tag="RD521_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDT7770120.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(73476..74372)
                     /locus_tag="RD521_000357"
     CDS             complement(73476..74372)
                     /locus_tag="RD521_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7770121.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(74365..75045)
                     /locus_tag="RD521_000358"
     CDS             complement(74365..75045)
                     /locus_tag="RD521_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7770122.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(75046..75828)
                     /locus_tag="RD521_000359"
     CDS             complement(75046..75828)
                     /locus_tag="RD521_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770123.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(75821..76738)
                     /locus_tag="RD521_000360"
     CDS             complement(75821..76738)
                     /locus_tag="RD521_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7770124.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(76902..77225)
                     /locus_tag="RD521_000361"
     CDS             complement(76902..77225)
                     /locus_tag="RD521_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770125.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            78013..81240
                     /gene="fss3"
                     /locus_tag="RD521_000362"
     CDS             78013..81240
                     /gene="fss3"
                     /locus_tag="RD521_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDT7770126.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            81353..81667
                     /locus_tag="RD521_000363"
     CDS             81353..81667
                     /locus_tag="RD521_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7770127.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            81680..82054
                     /locus_tag="RD521_000364"
     CDS             81680..82054
                     /locus_tag="RD521_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7770128.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            82064..82399
                     /locus_tag="RD521_000365"
     CDS             82064..82399
                     /locus_tag="RD521_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDT7770129.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            82482..83822
                     /locus_tag="RD521_000366"
     CDS             82482..83822
                     /locus_tag="RD521_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDT7770130.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            83900..84574
                     /locus_tag="RD521_000367"
     CDS             83900..84574
                     /locus_tag="RD521_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7770131.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            84567..84731
                     /locus_tag="RD521_000368"
     CDS             84567..84731
                     /locus_tag="RD521_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770132.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            84807..85949
                     /gene="dcm"
                     /locus_tag="RD521_000369"
                     /pseudo
     CDS             84807..85949
                     /gene="dcm"
                     /locus_tag="RD521_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            85939..86229
                     /locus_tag="RD521_000370"
     CDS             85939..86229
                     /locus_tag="RD521_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770133.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            86488..87672
                     /locus_tag="RD521_000371"
     CDS             86488..87672
                     /locus_tag="RD521_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7770134.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            87669..87806
                     /locus_tag="RD521_000372"
     CDS             87669..87806
                     /locus_tag="RD521_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDT7770135.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        25948 a        18101 c        15786 g        27995 t
ORIGIN      
        1 gcacccggta tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac
       61 actggcaatt ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct
      121 tatcaaaaaa taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct
      181 tcttttttat ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt
      241 ggatccctaa taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca
      301 ttacgaaaga aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca
      361 ctcctgcaat aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga
      421 tgctttttct aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca
      481 tgagccctgc tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga
      541 cacttaaata ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca
      601 gcatccctgc ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg
      661 aaatagaaaa actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg
      721 caacaatcgt tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga
      781 ataaaaaagt cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac
      841 aattcctacg ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca
      901 gcccaattgg actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg
      961 actaactggt ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact
     1021 tacttcatcg attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac
     1081 tagacgctcg atagctgaat cttctattct ttgtaaagca gggccggata atactggatg
     1141 tgtacatgaa gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt
     1201 gatcgtacct gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg
     1261 tccaatgata ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc
     1321 aatcggagat ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg
     1381 tgaaacaacg acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag
     1441 tggcgcaccc atcaaatgat cgactggaat atcaaagaaa ccttggattt ggactgcatg
     1501 aaggtccaaa gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt
     1561 cgctgtgatt ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg
     1621 catgactacg ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa
     1681 ctccatcaag ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc
     1741 acgaatactt tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga
     1801 ttttcctaac tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt
     1861 taacgcaaaa atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt
     1921 ctttttcaca actctagaat tctacctaat aaatagtagt catatttttt aaataaatca
     1981 agtgattttg caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt
     2041 gacttgtctt gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga
     2101 cccggcagca atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga
     2161 accaataaag ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac
     2221 gaagacaaca ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt
     2281 caagtgaccg actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc
     2341 gatatggacg ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc
     2401 tgcatgtttc ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc
     2461 aatcctactg tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt
     2521 atttccttgg atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc
     2581 atcgatgtat gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt
     2641 aattcgcttt ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat
     2701 tgattcttca aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat
     2761 ttcgatgata tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa
     2821 cgcatcgaac aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag
     2881 agcttcttct ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc
     2941 acggatgatt cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact
     3001 tgcatttttt ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg
     3061 tgtatcgcca ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg
     3121 caaaacagca tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc
     3181 tagttgtgct ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt
     3241 cgtttcactg acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa
     3301 aactttatat aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata
     3361 acgtgcgtcc aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat
     3421 acccattttt tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa
     3481 atagatagac cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg
     3541 gtcacaagtc acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga
     3601 cttagagtta aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct
     3661 tcggtattag ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa
     3721 tactcaggac aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt
     3781 ctcggtatta agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt
     3841 actactcgaa actaagcggc tttttcacaa cctcttattc tgaaaaataa tttcctggca
     3901 caaccgtaat atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat
     3961 catcgatggc gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt
     4021 ctgattcgaa ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga
     4081 aatcatccac tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct
     4141 tttcaattct ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta
     4201 tttttgagtc ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg
     4261 caataggtac gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt
     4321 tagaagcaat gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga
     4381 gataaacata tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt
     4441 caatatattt tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag
     4501 cacccgggat tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc
     4561 ccaaatcttc actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg
     4621 aaatcaattc atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc
     4681 gacgtacttt cacctattcc aactcctttt aagaaatttt atcgttttac attatataag
     4741 tttttcttca aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta
     4801 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
     4861 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
     4921 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
     4981 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
     5041 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
     5101 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
     5161 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
     5221 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
     5281 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
     5341 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
     5401 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
     5461 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
     5521 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
     5581 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
     5641 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
     5701 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
     5761 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
     5821 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
     5881 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
     5941 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
     6001 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
     6061 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
     6121 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
     6181 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
     6241 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
     6301 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
     6361 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
     6421 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
     6481 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
     6541 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
     6601 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
     6661 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
     6721 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
     6781 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
     6841 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
     6901 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
     6961 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
     7021 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
     7081 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
     7141 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
     7201 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
     7261 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
     7321 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
     7381 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
     7441 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
     7501 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
     7561 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
     7621 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
     7681 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
     7741 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
     7801 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
     7861 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
     7921 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
     7981 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
     8041 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
     8101 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
     8161 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
     8221 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
     8281 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
     8341 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
     8401 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
     8461 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
     8521 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
     8581 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
     8641 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
     8701 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
     8761 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
     8821 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
     8881 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
     8941 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
     9001 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
     9061 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
     9121 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
     9181 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
     9241 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
     9301 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
     9361 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
     9421 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
     9481 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
     9541 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
     9601 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
     9661 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
     9721 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
     9781 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
     9841 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
     9901 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
     9961 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
    10021 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
    10081 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
    10141 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
    10201 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
    10261 taatagccaa actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat
    10321 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
    10381 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
    10441 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
    10501 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
    10561 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
    10621 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
    10681 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
    10741 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
    10801 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
    10861 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
    10921 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
    10981 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
    11041 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
    11101 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
    11161 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
    11221 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
    11281 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
    11341 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
    11401 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
    11461 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
    11521 aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg
    11581 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
    11641 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
    11701 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
    11761 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
    11821 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
    11881 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
    11941 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
    12001 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
    12061 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
    12121 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
    12181 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
    12241 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
    12301 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
    12361 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
    12421 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
    12481 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
    12541 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
    12601 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
    12661 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
    12721 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
    12781 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
    12841 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
    12901 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
    12961 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
    13021 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
    13081 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
    13141 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
    13201 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
    13261 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
    13321 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
    13381 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
    13441 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
    13501 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
    13561 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
    13621 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
    13681 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
    13741 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
    13801 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
    13861 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
    13921 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
    13981 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
    14041 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
    14101 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
    14161 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
    14221 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
    14281 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
    14341 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
    14401 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
    14461 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
    14521 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
    14581 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
    14641 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
    14701 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
    14761 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    14821 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    14881 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    14941 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    15001 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    15061 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    15121 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    15181 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    15241 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    15301 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    15361 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    15421 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    15481 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    15541 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    15601 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    15661 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    15721 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    15781 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    15841 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    15901 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    15961 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    16021 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    16081 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    16141 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    16201 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    16261 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    16321 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    16381 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    16441 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    16501 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    16561 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    16621 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    16681 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    16741 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    16801 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    16861 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    16921 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    16981 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    17041 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    17101 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    17161 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    17221 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    17281 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    17341 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    17401 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    17461 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    17521 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    17581 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    17641 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    17701 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    17761 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    17821 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    17881 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    17941 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    18001 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    18061 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    18121 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    18181 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    18241 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    18301 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    18361 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    18421 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    18481 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    18541 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    18601 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    18661 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    18721 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    18781 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    18841 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    18901 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    18961 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    19021 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    19081 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    19141 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    19201 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    19261 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    19321 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    19381 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    19441 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    19501 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    19561 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    19621 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    19681 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    19741 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    19801 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    19861 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    19921 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    19981 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    20041 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    20101 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    20161 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    20221 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    20281 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    20341 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    20401 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    20461 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    20521 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    20581 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    20641 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    20701 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    20761 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    20821 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    20881 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    20941 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    21001 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    21061 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    21121 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    21181 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    21241 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    21301 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    21361 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    21421 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    21481 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    21541 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta
    21601 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    21661 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    21721 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    21781 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    21841 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    21901 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    21961 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    22021 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    22081 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    22141 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    22201 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    22261 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    22321 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    22381 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    22441 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    22501 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    22561 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    22621 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    22681 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    22741 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    22801 ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct
    22861 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    22921 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    22981 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    23041 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    23101 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    23161 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    23221 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    23281 tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt
    23341 tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc
    23401 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
    23461 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
    23521 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
    23581 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt
    23641 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
    23701 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
    23761 tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt
    23821 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
    23881 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
    23941 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
    24001 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
    24061 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
    24121 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
    24181 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
    24241 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
    24301 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
    24361 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
    24421 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
    24481 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
    24541 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
    24601 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
    24661 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
    24721 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
    24781 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
    24841 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
    24901 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
    24961 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
    25021 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
    25081 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
    25141 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
    25201 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
    25261 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
    25321 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
    25381 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
    25441 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
    25501 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
    25561 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
    25621 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
    25681 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
    25741 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
    25801 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
    25861 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
    25921 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
    25981 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
    26041 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
    26101 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
    26161 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
    26221 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
    26281 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
    26341 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
    26401 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
    26461 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
    26521 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
    26581 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
    26641 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
    26701 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
    26761 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
    26821 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
    26881 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
    26941 gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
    27001 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
    27061 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
    27121 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
    27181 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
    27241 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
    27301 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
    27361 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
    27421 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
    27481 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
    27541 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
    27601 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
    27661 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
    27721 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
    27781 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
    27841 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
    27901 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
    27961 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
    28021 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
    28081 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
    28141 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
    28201 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
    28261 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
    28321 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
    28381 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
    28441 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
    28501 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
    28561 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
    28621 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
    28681 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
    28741 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
    28801 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
    28861 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
    28921 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
    28981 caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga
    29041 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    29101 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    29161 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    29221 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    29281 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    29341 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    29401 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    29461 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    29521 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    29581 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    29641 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    29701 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    29761 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    29821 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    29881 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    29941 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    30001 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    30061 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    30121 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    30181 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    30241 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    30301 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    30361 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    30421 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    30481 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    30541 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    30601 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    30661 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    30721 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    30781 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    30841 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    30901 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    30961 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    31021 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    31081 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    31141 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    31201 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    31261 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    31321 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    31381 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    31441 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    31501 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    31561 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    31621 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    31681 atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg
    31741 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    31801 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    31861 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    31921 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    31981 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    32041 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    32101 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    32161 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    32221 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    32281 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    32341 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    32401 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    32461 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    32521 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    32581 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    32641 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    32701 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    32761 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    32821 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    32881 cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca
    32941 tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag
    33001 taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt
    33061 acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat
    33121 tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa
    33181 caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt
    33241 ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg
    33301 taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc
    33361 aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg
    33421 tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa
    33481 gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg
    33541 tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata
    33601 atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt
    33661 ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc
    33721 gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat
    33781 ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca
    33841 gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa
    33901 aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca
    33961 acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct
    34021 acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca
    34081 taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca
    34141 gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga
    34201 cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa
    34261 aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac
    34321 agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga
    34381 agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg
    34441 cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt
    34501 gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca
    34561 acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt
    34621 atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc
    34681 ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc
    34741 ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga
    34801 tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac
    34861 tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc
    34921 acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc
    34981 tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg
    35041 ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt
    35101 gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg
    35161 cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt
    35221 ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt
    35281 ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct
    35341 tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt
    35401 tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt
    35461 atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat
    35521 tacgctactg attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca
    35581 cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg
    35641 gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc
    35701 catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag
    35761 cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt
    35821 catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg
    35881 atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat
    35941 tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa
    36001 ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat
    36061 tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga
    36121 gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt
    36181 cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt
    36241 gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg
    36301 ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt
    36361 ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt
    36421 gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    36481 tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg
    36541 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat
    36601 ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct
    36661 tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata
    36721 agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca
    36781 cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa
    36841 gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata
    36901 attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt
    36961 ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga
    37021 taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc
    37081 acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat
    37141 ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact
    37201 gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt
    37261 ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat
    37321 gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg
    37381 ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta
    37441 ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca
    37501 ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca
    37561 gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta
    37621 gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac
    37681 agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct
    37741 actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt
    37801 ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc
    37861 atccctttct ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta
    37921 aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta ggatttttat
    37981 gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga
    38041 attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct
    38101 tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa
    38161 aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga
    38221 aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct
    38281 cgataaaaca acagctactg gactgctatt cactagagaa caacagctga acaaaatcat
    38341 gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg
    38401 ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca
    38461 agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat
    38521 atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg
    38581 atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa
    38641 gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat
    38701 tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg
    38761 gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat aattgatcag
    38821 gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca
    38881 tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa
    38941 aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga
    39001 atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat
    39061 tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt
    39121 cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag
    39181 cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact
    39241 tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga
    39301 tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt
    39361 tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc
    39421 gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag
    39481 tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga
    39541 atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact
    39601 ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta
    39661 cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca
    39721 tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag
    39781 cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc
    39841 ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg
    39901 gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc
    39961 tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc
    40021 gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt
    40081 ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct
    40141 ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta
    40201 acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt
    40261 ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga
    40321 cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc
    40381 agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag
    40441 gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa
    40501 agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc
    40561 cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca
    40621 aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta
    40681 tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa
    40741 aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg
    40801 tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat
    40861 tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat
    40921 ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa
    40981 aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt
    41041 ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa
    41101 taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga
    41161 atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag
    41221 ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt
    41281 aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt
    41341 cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca
    41401 tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga
    41461 tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag
    41521 aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg
    41581 tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt
    41641 gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat
    41701 ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg
    41761 gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt
    41821 agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt
    41881 gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc
    41941 aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta
    42001 agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt
    42061 tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac
    42121 tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct
    42181 gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat
    42241 gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa
    42301 actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc
    42361 gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata
    42421 aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg
    42481 catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt
    42541 tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct
    42601 attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta
    42661 tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca
    42721 ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca
    42781 gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt
    42841 tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga
    42901 taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga
    42961 cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg
    43021 attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa
    43081 gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg
    43141 cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac
    43201 gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa
    43261 gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta
    43321 ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg
    43381 actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca
    43441 atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt
    43501 tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg
    43561 ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg
    43621 gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag
    43681 acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc
    43741 gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact
    43801 gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc
    43861 ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa
    43921 tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag
    43981 acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact
    44041 atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg
    44101 gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag
    44161 cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag
    44221 cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga
    44281 tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga
    44341 cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag
    44401 aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa
    44461 ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac
    44521 cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac
    44581 cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg
    44641 ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt
    44701 ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt
    44761 caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc
    44821 tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta
    44881 ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg
    44941 tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa
    45001 tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga
    45061 ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga
    45121 ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga
    45181 acatcacttg acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg
    45241 tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta
    45301 gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt
    45361 cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt
    45421 tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt
    45481 tataacggac ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac
    45541 tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga
    45601 cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata
    45661 tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac
    45721 ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa
    45781 aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa
    45841 tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg
    45901 atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga
    45961 atgcagatga tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg
    46021 aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac
    46081 ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga
    46141 aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc
    46201 taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt
    46261 ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc atcaatactt
    46321 atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca
    46381 tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg
    46441 agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt
    46501 taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct tccttttatt
    46561 tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa cataactgtc
    46621 tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat
    46681 gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc
    46741 cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa
    46801 aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt
    46861 agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga
    46921 tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa
    46981 tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt
    47041 tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc
    47101 tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt
    47161 gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct
    47221 tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg
    47281 aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat
    47341 ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta
    47401 ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc
    47461 tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat
    47521 ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga
    47581 actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt
    47641 taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt
    47701 caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg
    47761 gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta
    47821 ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca
    47881 ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact
    47941 gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag
    48001 atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt
    48061 ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca
    48121 atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg
    48181 gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca
    48241 ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac
    48301 tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa
    48361 ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg
    48421 cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca
    48481 gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt
    48541 gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg
    48601 atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga
    48661 tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc
    48721 gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa
    48781 ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg
    48841 actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga
    48901 gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat
    48961 acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc
    49021 gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg
    49081 gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca
    49141 gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg
    49201 attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat
    49261 gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt
    49321 ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat
    49381 atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt
    49441 caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca
    49501 ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa
    49561 atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc
    49621 tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat
    49681 cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata
    49741 agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct tattacttga
    49801 tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat
    49861 gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca
    49921 aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat atttctttta
    49981 atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa
    50041 tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt
    50101 gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt
    50161 ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat
    50221 tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt
    50281 ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt
    50341 gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc
    50401 gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg
    50461 gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata
    50521 ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa
    50581 cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat
    50641 catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt
    50701 cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg
    50761 cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct
    50821 attcttttta tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa
    50881 gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct
    50941 ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga
    51001 tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa
    51061 atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct
    51121 tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt
    51181 aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat
    51241 atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg
    51301 gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga
    51361 ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt
    51421 tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg
    51481 cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg
    51541 gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt
    51601 tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc
    51661 atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca
    51721 tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat
    51781 gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga
    51841 ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct
    51901 gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa
    51961 agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt
    52021 tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca
    52081 attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca
    52141 tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca
    52201 cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg
    52261 caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct
    52321 cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc
    52381 aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt
    52441 cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa
    52501 tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt
    52561 tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg
    52621 tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt
    52681 ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt
    52741 ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc
    52801 tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga
    52861 ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc
    52921 gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa
    52981 acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct attattttat
    53041 aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa
    53101 gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact
    53161 tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat
    53221 tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt
    53281 agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt
    53341 attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct
    53401 gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa
    53461 caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa
    53521 gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa
    53581 ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac
    53641 ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta
    53701 gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca
    53761 tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc
    53821 tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga
    53881 ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta
    53941 cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc
    54001 ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca
    54061 atttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt
    54121 aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc
    54181 gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca
    54241 aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt
    54301 ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg
    54361 gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt
    54421 ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa
    54481 agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa
    54541 agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc
    54601 taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa
    54661 gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt
    54721 tttataagtc atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga
    54781 ttttatgata cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa
    54841 aattctgaca acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa
    54901 aataacgatt aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat
    54961 atactaaata gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt
    55021 accaaaaaat gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga
    55081 aattactggc tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa
    55141 attatgtttc attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta
    55201 aatatataaa cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc
    55261 cggctgttgc taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta
    55321 catgattaat taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg
    55381 caagcacaaa gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag
    55441 gttcgcttat attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt
    55501 ttctacgact gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca
    55561 ttaccgcaaa ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg
    55621 cataagctgc catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg
    55681 atattactgc ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca
    55741 aaaatcctgg taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta
    55801 gcttatttat atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg
    55861 taatcgtaat aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat
    55921 ttccggatag ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag
    55981 ccgctaatcc aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta
    56041 tcacacccgt cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag
    56101 gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat
    56161 ttctgcttgc acacatatga taagatactg ctgctacaac taataatgta gttatgctta
    56221 atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga
    56281 caatttcccc catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc
    56341 ctgctaacat cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga
    56401 tctgattccc caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc
    56461 tacttttgcc catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca
    56521 aataatacat cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt
    56581 agtataaaaa aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca
    56641 gcttcaaaaa caaagtcttg atataaatgt ctcgataccg aatactcgaa aggtttacca
    56701 gacgatagag caacaacttt gtctatctca atcataaatt cacctggatc tatttttaat
    56761 atttttgcgt caaattcatt cgcagtatct cctttgatac gtatgtgcga agtctttatt
    56821 tccagaccca gttcatttgt gatataagca tatacagatt tctttaaatg ttttggttcc
    56881 aagcctggta tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg ttgacaatca
    56941 ataaaacggc ttcgtacaat attatataca tagtctttta cactgatact taatttatca
    57001 gctacttttt tcgatggaag ttcaattgag aaagtatgga tttctgaatc tacgtgttct
    57061 tcaccaacca cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt atcaattggt
    57121 gttctgacga cttcagtccc atacccactt ctacttcgca aaactccttc ggaaacaagt
    57181 aagtctacgc ctttctttac tgttaacttg cttgtatgat aaataactgc caattcatct
    57241 cctgtaggaa gtttctcacc aattttatac tctccattta caattttttt ctttatatct
    57301 aagtaaattt ccatatactt cgcaattttt ttcaatggtg accctccaat caaaaacata
    57361 tcttcatcta tactttaaca taaataaacc gataaaatcc acatcaaaat tatacacaca
    57421 ataaaaaaga tatatatatt taaacaaaac tatttacttt aagttgtaag cggtgttatc
    57481 attaagtgga aacaggaggt aaaaaaatta tgacaactat gaaaaagcta acttttccag
    57541 atgatttttg gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag
    57601 gaaaagcaga gactatctgg gatcgctggt ttaaagaaca accttaccgg ttttatcaac
    57661 gaatcgattc ctctattaca accgatcaca tccatcaata taaagaagat atagaactaa
    57721 tgaaaaaaac tgggcataac tcctttcgcg tttctatttc ttgggcacga atgtttccaa
    57781 atggcggtac cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa ttattttccg
    57841 aaatgaataa taacggaatc aaagtatttg ccaacttata tcattttgac atgcctgcta
    57901 ctcttcaaga taaaggagga tgggaatcac gtgaagtagt tgatgcttat gttcattacg
    57961 ctgaaacttg ttttaaagaa tttggtgact tagtttgtca ttggtttacc ttcaatgaac
    58021 cacttgggcc tattttaggt acttatcttg aagattttca ttatccaaac ctcattgact
    58081 ttaaacgcgg agcgcaagca gcattcttca ctatacttgc acacgcaaaa gcaatacaag
    58141 tatttaaaga actagctctt cctagtaaaa taggcgttat tctaaatttg agtcctactt
    58201 ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga aatcgctgat ttattttata
    58261 cgcggagctt tttagatcct atggttaaag ggaatttttc taaaaagctt atcctgttat
    58321 tacaagaata taatcagctt cctgaagatt atacgaaaga agatcttgta tgtatctacg
    58381 aaaataccgc tcaaattctt ggactaaact attacgagcc acgtcgcgtc aaatctcgtt
    58441 cttctatggt ggatacatct agtccatttt taccagagtg gtttttcgaa ccacatatca
    58501 tgcctggacg acgaatgaac gaataccggg gatgggaaat ttacgaaaaa ggaatttatg
    58561 atttgtgcat ggatatcaag aaccattatg gaaacatcga agcatttatc tcggagaacg
    58621 gaatgggcgt tgcggatgaa gaacgtttca tggatgatac agggcaaata attgatgatt
    58681 atcggattga gtatatcaaa gatcatcttg cttacttatg gaaggcaata aatgaaggat
    58741 gtaacattaa ggggtatcat ctttggacat ttattgattg ctggtcatgg atcaacgcct
    58801 ataaaaaccg atatgggctg gtttcactcg accttactac tcaaaaaaga acaattaaaa
    58861 aaagtggcga attttacaag caattaagtg atacaaatgg atttagatac gatgtagact
    58921 tgttagttga ataatattct aaaattccac ttgaagtgaa cacattagaa aacaagaata
    58981 atgagaaata ccaataacaa atactttttc ttcatacttc atttatactt gtcagtataa
    59041 gagactgtga caaatgtagt gatcctcaga agttaggtct ggaaatctaa cttgagaaga
    59101 tttttttaaa ctacgtccag tcttttttat ttgtttaaaa ttgaagttga caagtcttca
    59161 ttaaaaattt agaatattca caagtttgac gacaattatg aaaatttatg tcgtgctaaa
    59221 ggagttttgt tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct
    59281 accgtttttg gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccggg
    59341 tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct atgctggttc tgcccaattt
    59401 atcacggtca gcatgcttgc tagccatagt ccactgctct ccatcgttct ctcgaccttt
    59461 ctagtcaatt cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat
    59521 cggctgcttc aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga
    59581 atgaataaat tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat
    59641 tggatttctt atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt
    59701 attactgatc cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta
    59761 ctttatcttc aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt
    59821 ggtctcactt tgattctggt atatgtcgga ctgattttta tcccaagtaa cttagtgatt
    59881 ttagttgtaa cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga
    59941 gtttacattg ctgactattt taggttgtac tttagcaact tggctttcta ggatcctacc
    60001 atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt taagttttgt
    60061 cccgatcgtc attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca
    60121 tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat
    60181 tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg
    60241 cttattataa ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt
    60301 atcaaagtca aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta
    60361 acaacgactt ttcctaatat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc
    60421 ttcgcattca tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt
    60481 gctaaagcag caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct
    60541 gttttagcga acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata
    60601 tctaacggct ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt
    60661 ttgatttgat caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg
    60721 attcctaatt cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca
    60781 cctgtttttt tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca
    60841 gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt gatcactaag
    60901 atttttttcc cgcggttggc attttcttct ggagtcagac caaatttttc aaataacaat
    60961 tcataagcgg tcaatgaggt caacggcaag gctgcggctt cttcatctga aagatttttt
    61021 ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt cttgattgct tcctgctctt
    61081 gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcatcacct
    61141 acagcaacaa ctttcccgac tccatcaaac cctaagatac gtaaagcatt tcttatcccg
    61201 ttatcttggc gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga
    61261 agatcccgtg aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca
    61321 tcgatcgaca acccttcata aaaaccgact accttcatta attccttttc tgacatacga
    61381 attcctcctt agcttcatca agttggagca tccatacagt tttcatttca atatatatag
    61441 tatgctattt gttccacttt tattcatcat aattattctc ttacttaaag taaactattt
    61501 gcactttttt ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttttaaacg
    61561 gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg acaaaatatg aggagctatt
    61621 tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacgacct ctaattcacg
    61681 gtgttcaagc aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtgt
    61741 ctaagatctc tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat
    61801 ttgctcctgg aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac
    61861 gcgggatgat ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca
    61921 gttctttttc tgtatgttcc ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc
    61981 cccggccaat catgatgcca tctacgccat atttttcggc caattcttct cctgtctggc
    62041 gatctgggat atctccgttg atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag
    62101 caatgatttt agggatgact tcccaatgtg catctacttt actcatctct ttacgcgtac
    62161 gcaaatggac agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca
    62221 tctcagacat ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt
    62281 tagccgcttc aatcaattgt gccgctactt ctgggcgaag gatcaagcca ctgccttttc
    62341 cacgttctgc tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca
    62401 tttctgccat agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg
    62461 caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg tcttttccat
    62521 ctggatgaca gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg
    62581 catgctttac gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa
    62641 aaaaaggctt tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc
    62701 tatcaagcag tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa
    62761 aaagaagcaa agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca
    62821 aaaatcttat cacaattcct tttccggaat aaatggtgtt caaaagcttg catctgcggt
    62881 aataagtcca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
    62941 tgatgcagaa cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc
    63001 ggtattagct caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata
    63061 ctcaggtcaa aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat
    63121 tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg
    63181 atgttgctac tggcacatcg attgttccaa ggaatccgta acggaaaccg aagatcatat
    63241 aaacgatcgg attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat
    63301 agaaaacgcc acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat
    63361 cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg
    63421 tcatcaataa tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca
    63481 aggaaatgat cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa
    63541 agccccaaat gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct
    63601 ggaatttttg agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca
    63661 tcataatcaa accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc
    63721 gaccaccgat catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa
    63781 ccagtgtttg gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta
    63841 atgcagtaaa ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt
    63901 ttcaaaaaca gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct
    63961 tgtcttgtca gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg
    64021 attgtctgtt catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat
    64081 ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta
    64141 gtattctcga tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct
    64201 tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga
    64261 aactcccaca tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc
    64321 agcaaacgtg gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg
    64381 gaaagcatac gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt
    64441 tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg
    64501 atttgctgga ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt
    64561 tgctttgctc ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat
    64621 gttttattga ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt
    64681 cctaatagcg catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg
    64741 accccacttc cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc
    64801 ctatctcctt atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa
    64861 cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc
    64921 aatcggacga agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat
    64981 tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
    65041 aaacacgtat aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat
    65101 cacttcgcct atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt
    65161 tttgcggttt ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt
    65221 cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt
    65281 acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt caattcgata
    65341 tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg
    65401 gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag
    65461 cagcgttctt tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca
    65521 aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca
    65581 ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg
    65641 atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta
    65701 ttcttctgcc agttgaccgg attcactacc gcttctttta acaatggatg atagatatct
    65761 ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg
    65821 taattcaata ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct
    65881 tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg
    65941 aacatgatgt tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat
    66001 gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga
    66061 agtggtgtcg gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc
    66121 atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat
    66181 actaacccac cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa
    66241 cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt
    66301 tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat
    66361 tgatattgga ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct
    66421 aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct
    66481 acactagaat aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa
    66541 gtaagatcat cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc
    66601 ttcaagcttt cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga
    66661 actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct
    66721 gcaataataa ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt
    66781 gttagcttta gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa
    66841 aataaaaggt gtttttttct tgtatattac atatttatct ctcatttatc aaaattgaca
    66901 aataatcata gggaggaatt gttgttaatt cttgtttatt ctccagtgca ttgacaactg
    66961 tcccaaggga aaggatagcc tcaggtacta ttttttcttg ttctgttgga ttagctaagc
    67021 gaagaacaaa tccttgttca tgaaaataag aaggataaat cgatgataca attaagtctt
    67081 caggtaattc aatcaatgaa aatttacgag gaacatcaag ttcattttgt aatggccaaa
    67141 tcttgttatc taacctgtga ataaatacat tcagtttctg cttttgataa gaaatagatt
    67201 gttctaaccg tttttctgcc tgttttgcca ctttgaactc attaaatctt ccttcttcta
    67261 cattaaatcc aaaagtgact ttatactcac ctatctcttg agctagcggt gtttccatca
    67321 tgacatgccc ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa gcaagattag
    67381 gttttccaag ttcccctgtt gttgaaaata aggttatgat taattgattt tctatacgtt
    67441 catactcttt taaaccatct gcataaaacg taaaataatg atcattttct gccacagaga
    67501 cactcttatc gaatatttct aaattaacag gtttctctac catagtttct gaccaattcg
    67561 gaggaatgac atcaggggta ttttcaataa atccgttttg aatttgtgca attgattttt
    67621 cagatttatc taaaacatta attgctaagc gcatacgatg cgagtaaaca gtattatgaa
    67681 ttttgagtgt gccttcaacg atttgacttt ctcccagact gaacgatatt tcataagata
    67741 tttcttccat ttcgggtttt tcagataaac gatcttttaa atctttagga agttgagctt
    67801 ttccgtaaac aactaatgtt tcttgtaaag aatctttata tactttagct gtttctaatt
    67861 ttagagatag ttctgtatct ccttctaatg gtgaataatc atacgtatcg ccatcatttg
    67921 ctgaatcgat caaatgaacg aaatcaagat attgtctatc tccaactatc aaattcaatt
    67981 gcccatcttt aaaagaaagt ttatatttat tgttacttat ctcagttttg tctatctcga
    68041 ttgctgtttc taatttttca ttatttttct caaattcaac aacagtgtat cctagtgctg
    68101 gcacatttac atttattttt atgtcaagtt cataataagc tggttcatca aaatattcga
    68161 aacctgtagc aatcatccga taagcatttt tccgttctgg aaaataaact tctttttcaa
    68221 tgatattata gggactacct ttgaagtgta ttttctttga atcagctact acatgcacga
    68281 ttttcgatcc actataattc tcagtcgttg tgttgaaaac aaggacttca ttcgatttta
    68341 gctttaatgc atcagccatc cgttttacaa taaggttttc gattccgtca gctatctcat
    68401 tagcttcttt gattcgatgg aaaatatctt ccgcaacatt atctgaaaca cagccaccaa
    68461 tactatcatg agcctggttt tgcaaaatct ttttccataa ccgacgaagt aggcctttac
    68521 tgatatggat accatttctt tctgcaatca gcatgagcgg ctcaactcgt tttagaattt
    68581 tttgttccaa tttaaaattt tctattttca tttttgttct tactgagcca atagaacgat
    68641 gaacacgaga gtaaaccggc tgacgaagtt cacctttata ttcaggtaat tcttttttgt
    68701 ttctgataac ttctacataa gaaggataat ctgaaataat attgtggaat ttactgtatt
    68761 tattcaactt ctctacgact tgatcaaaat ttaaaatcat tgacttttga tctacacccg
    68821 aaggtactaa aatatctttg ttttttcctt gcatatgaag catttcaact gcttcatcca
    68881 acttttcttt tgcaaattca ggaatatctt taattgcgtc taaggtcatc attccagtca
    68941 tataaccaaa tggaaaattc atggcataaa caaatttatc tcccaatcct ttccagataa
    69001 aataaggaga tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg aaattatcga
    69061 tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg tcaggcaaat
    69121 accctaccat cattggatct ccatatttaa ggatcgtatc actgattcca atcattaagt
    69181 ttcttaaaat tgattcagca tccactaata aggcatctgt ttgtgcatac cacggtccca
    69241 cgaatagttg tcctgttttc atcagtcgtt ggataacctt aagcttttgc ggattgatct
    69301 ccaaatactc atcgacgatt gaagtttgtc catctaaaca aaagtttact ttagggttcc
    69361 tttccaattc atctagaact tcagtaaaga cctgatcaga caatacgatg gcatcttcat
    69421 ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat tatatgtgca tttaccatat
    69481 cagattctcc tactttcctt cttctactga atttacttgt tgttcttttt tatacttgat
    69541 ggattttagt gcattgacca ttaacgctgt aaccactccc ccaataacaa ttgatactaa
    69601 atataaaggt atattcgtaa caaaaggaat tgccataaag gttgaaagca ttgtttcttg
    69661 agtaacacca aaaaatccat ttagaccaca tgctactgca gtgcccacca cgatgcttgg
    69721 tattactcgt aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa tccccacaag
    69781 gcccataatc atagctgtct ttgcattatc tcgttccgat tcatcataaa gttttttgtt
    69841 taaaattgtt gctaaaaaca ttccaaatgg cggaatagca attcctaata acatggctgt
    69901 actaggtcca aagcttccgc ttgaaaaaga agcaacccca aaagcatatg cgattttatt
    69961 gataggtcct cccatatcaa acgccatcat tcctcctaaa atagccgaca tcaaagcagc
    70021 atttgctcct gacatgcctt ctagccaatt agtcagtcct gttgtcattg cttgaatagg
    70081 cgtagctagt acataccata atatgattcc aacaactaat gtagtaacaa aaggaatcag
    70141 cataattgat tttagactaa taacttgcat cggtactttt acttttttca atgcattagc
    70201 taaatagcca caagctaatc cagcaaccat tcctccaata aatcccatac caagattttg
    70261 acacatcatc ccagtgataa aagctggtgc tagaccggga cggtcagcaa ttgaatatgc
    70321 gatataggct gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa tcattcgcag
    70381 gactccccag aatgtatccg tctggtccca aacacctgtg ccaccaaaaa tgactgcaat
    70441 agaagttagt aaccctgctg ctacgacaag cgggatcata taggaaaccc cactttggaa
    70501 atgatttaga atttctcttc cttgtttctt gaattccatt agtaactccc ccacgatata
    70561 ttttttattt gttaattaat gcaattgctt catcaatcgt ctcttcacca tcttttacta
    70621 cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt tctgaaacat
    70681 ttgtatctac tgcaaatatg acaacatctg cctcggaaac atccgaaatt gaaagtttgt
    70741 tttctatccc catagccccc tgtgtttcta ctttaatatc taaccctttc ttcttcgcaa
    70801 acttttctaa attagctttt gccatataag tatgggcaat ccctgcgata catgctgtta
    70861 ctgctactat ttttgtcata tgaaattcct ccttttaaaa tgatagagct tttaccaatt
    70921 cttttttatt tctcgaatct tttattcttt taacaaactc gtcatccatc aatcttccag
    70981 ataattctgc taaaatacgt aaatgattag tacctttatc agtgtctttt attgctaaca
    71041 aaaagacgag atcaactggt tgatcatcta atgcgcccca ttcaacagac cgctttaact
    71101 tggctacagc cactgttgat tcaattactg ctaggctttt gccatgagga atggcgatat
    71161 ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt tgaaattgtt
    71221 tgtggtcaga cacaaaccca ttagtctcta acaatttcga taatttttcg actacacttt
    71281 ttttatcaga tgcatttaaa tctatcacta tattatttat attgattttt tcactgatca
    71341 tttataagcc ttctttcaat atataaacta atttttcaaa cgacgaactc gtttttaaat
    71401 ttgctaataa ctcatgattc gaagcgattt cattgaatgt ttcataaatt ttattcaatt
    71461 caggttttac tttttcatta agtgcaaaaa agaaaacaga atgaacaagt gtcccatccc
    71521 aatcaattcc ttctggatta acgtagacac agattactgg cttttttata aaatctactt
    71581 cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga tagtttttct cttttgattg
    71641 ctccctctag tatcccttct ttcgcgtaat tttcttttac caattttttg gtaattatat
    71701 ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa aaagcaaaat cgtttatcta
    71761 ataaatttag aaaagcatct tctgtttgtt catttaactg tagatactgt tttacttttc
    71821 tgacatctgc ccctaacatc aaaggactaa caacaataac aggaaaattt gtattttcaa
    71881 tcggaatcgt gcttataaca agttcatcag tgacttttct atcttgtagt tcattgatgc
    71941 ttagtacatc ttctatctca accatatctg caaaaccgtt atttattctt tgctctaata
    72001 attttgatgt tccatatccg ctactacaaa ctaatatcac tttcgttttg tcaggtttgt
    72061 aacgatccaa aagtgattga atatggagtg ttacgtaagc gatttcatct tcattaaaat
    72121 gtatacagaa tctctcttcg atttcctcta ataaatctac acttatcata aatgaccttt
    72181 taaaagaata tttgattttg tccgtataag ggttatagat atttactccc aatttgagtc
    72241 ttttaactgc tgcattgaga tgtaatacta gattattgat caattcttca tcaggataaa
    72301 attcatttaa tttaattctt actaactctt gtagctcaac acccatatct aaataggaaa
    72361 gcttcgtttg atctaagtcg tgattcatag catttttttc aatcgaagta atgtgaatgt
    72421 caacataagc ttgttcatct attggcacaa taattgaaaa tgctttttca atcattttga
    72481 ttaaaataag tgtattcttt gagatttctt cttcagaatt attactacgt gataagaaat
    72541 tgcttgtttc aatcctttct atcgcaatag caaggtggat agctaaagtt tgaaattcat
    72601 aatctgtaaa tttcaattgt gtcttattca tgtatcgatt aagcaattcg gctacttttt
    72661 tcaatatgtc cttatttaag aaattattcg cttcaccaat gaaattgata ttcaagtcta
    72721 agagatctgt tttactgtta gatgctgtaa agccacccca ataatagttc acaacttctg
    72781 acatcaacct tcttttactt ttttcatcag ctttgatgcg cacgccattt cgatctgaaa
    72841 ctaaggacat cccatgcttg tttagtcttt ttctaacatc tttcattgtg ctttctaccg
    72901 tcgacctgct cacgtataat tcgtcagcaa gattttgaat ctttagagaa tcattagtat
    72961 tcaataattt agcaaacaca taagttagac ggtcctccgg agagtctgcg atattgattt
    73021 tatgactgga taataaattt cgaagtgttt ctttttttcc aataagcgaa acgccttttt
    73081 taggttgaac caacatttgt acatctggca ttttttggat tatttctttc aaaattaaaa
    73141 tatctttgct aattgttttc gtagatacat ataattgttc tgataattct ttgtaagttg
    73201 tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg ccgttcgcta aattcgatgc
    73261 ccattatctt cacctcctga atacagctta agtgtacaat tattatctct attttcatac
    73321 agaagaaagt tcttgattaa tgtagaagtt tctaatattc ttgattattt caaatcaata
    73381 aaaaagcctg caatatttat atcacaggct tttttctcta ttaaatatat ataagataat
    73441 acatttttct actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag
    73501 atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat
    73561 ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg
    73621 gtataaaaac gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg
    73681 gccactgtta atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct
    73741 gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct
    73801 gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa
    73861 aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg
    73921 agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata
    73981 ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta
    74041 tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga
    74101 tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt
    74161 cctttttgtt cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc
    74221 ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg
    74281 attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag
    74341 ataacaacga gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg
    74401 aatatacaca gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac
    74461 tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga
    74521 aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa
    74581 atggacagcc acaccattca catttacatc cctcgactct ttgtccagta agatttcctt
    74641 gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat
    74701 gccgattcgt aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc
    74761 tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg
    74821 aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat
    74881 gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc
    74941 tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac
    75001 agcttcatcg tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga
    75061 tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta gactgaccag
    75121 aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca
    75181 cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg
    75241 aatgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct
    75301 agtgatgaat gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac
    75361 aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc
    75421 gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt
    75481 gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga
    75541 taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc
    75601 tgcgaaagcc agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc
    75661 gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca
    75721 ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt
    75781 acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc
    75841 cagttaattg taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag
    75901 gaacttttgc ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa
    75961 gctggataac acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg
    76021 ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca
    76081 attcttcttt cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg
    76141 cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt
    76201 ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg
    76261 tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac
    76321 gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca
    76381 tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac
    76441 gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca
    76501 tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa
    76561 tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca ttttccccaa
    76621 tcaatccata gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag
    76681 caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac
    76741 ttatccttct ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag
    76801 aaatgtataa agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata
    76861 cgtgtctttc ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc
    76921 ttttcgacca aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga
    76981 tcaagcttga gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc
    77041 agaaaaatta gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca
    77101 aacagtgcta atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataagt
    77161 cgtccttgta tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa
    77221 aacattaatc cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat
    77281 tagctaccaa ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac
    77341 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa
    77401 tttttgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg
    77461 gataaataat attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga
    77521 tattatagac caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc
    77581 aaattgtgga aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc
    77641 ccagtacgat tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac
    77701 tgtgaagctt tacgggcttt cttaagacct ggttttttct tcgtatcatt gaataagttt
    77761 gaaaaacctt gatttataaa ggttttcccg taaatatatt ttattatatt tgattaaatt
    77821 tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc agtatatttc gtattttaga
    77881 ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa aaattttaaa
    77941 aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct taatttgaaa
    78001 ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac tattttgagt
    78061 ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc tgatgaaatt
    78121 acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc tgttgaagga
    78181 acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc agtgtacaac
    78241 ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa tgagttcact
    78301 cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt agtttccgtt
    78361 ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa gatgatctgg
    78421 caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag tgccgtaaat
    78481 atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa gaaaccaacc
    78541 ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac ggatacgaat
    78601 aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat tgattatcgt
    78661 gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag tggtgtgctg
    78721 acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc tggccaacag
    78781 actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat tgatgtggaa
    78841 acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt tgtaccagga
    78901 acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac tactgacaaa
    78961 gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat taccgaaaaa
    79021 tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat taaagcaggc
    79081 gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat tctggataaa
    79141 agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc tggtaatacg
    79201 tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac cacggatgaa
    79261 aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg aacctattat
    79321 gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt aaaagtcgaa
    79381 ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg gcaaaatcaa
    79441 gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga taaagcacaa
    79501 ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga tggaaaagct
    79561 gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg ttctgatgaa
    79621 acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt agctatcaac
    79681 gagtattatt gccaagaaac aaaagcgccc gaaggatata gccttgatga aacaaagtat
    79741 cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac ccgaaatgtt
    79801 acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc tggatcagct
    79861 gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc attggaaggg
    79921 accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga gcaattaggg
    79981 tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact tgaagaagta
    80041 gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac ttttaaggaa
    80101 aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca agaccaaaaa
    80161 caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc attttctgtt
    80221 agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt aacttctctt
    80281 gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga gctagttgat
    80341 aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc gcaagccatt
    80401 gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc agtggtgatt
    80461 gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa aattcagcat
    80521 aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta tgtgtatgaa
    80581 aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag cttgaataac
    80641 caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac aaaggcccac
    80701 ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt tgatgatgtg
    80761 tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat tctgtatgct
    80821 ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga ttatgaagta
    80881 aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg aaaatatcca
    80941 gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa tatcaatgga
    81001 aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga agttccaacc
    81061 atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc tcaagaatct
    81121 agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt tttactgttc
    81181 attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg ccggaactaa
    81241 ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc tcctaaaaaa
    81301 gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga agatggaatt
    81361 gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg ccggtgaagg
    81421 agaaattata acagaaggat atggacggaa tactacggtg attggtcgca gttatcattt
    81481 gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg aagccggtga
    81541 aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg ttgccaaagg
    81601 ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg atttagtgaa
    81661 agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt caggtttaac
    81721 atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa atgaggatgg
    81781 aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg cacaagggcg
    81841 aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt atgatgcaga
    81901 ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc gagaaggaac
    81961 taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc cgaatcaagg
    82021 aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa ttaatgttga
    82081 acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg aacaactcgt
    82141 taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt tacttggaga
    82201 aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag aagagtcttt
    82261 ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca ttttggaaga
    82321 aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg ttgaaacgaa
    82381 acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt gcctaatgaa
    82441 atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa ggacatcgca
    82501 tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta ctcgctctgt
    82561 tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat atttctaagc
    82621 tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt ctgcttggaa
    82681 gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt gatcattggc
    82741 gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat tggtatgaag
    82801 tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga accagagaga
    82861 ctatcagtta cttccccaaa atctgttatc gaatgaaaga tggattactg tctattcgcg
    82921 ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag aagttagaaa
    82981 gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg gagtatactt
    83041 tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca gaaaatggga
    83101 ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat atgttaatcg
    83161 ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa gcactattga
    83221 agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct gatttaggta
    83281 cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg gaagatttct
    83341 atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac aaaacaggag
    83401 agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa tacgtggctt
    83461 atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc aaacaaatcg
    83521 tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg ccggatgcga
    83581 aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg ggtcgtatga
    83641 gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca
    83701 tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt tatacgccac
    83761 ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag atgaatgatt
    83821 gagtgatctt caatactgac tttatacttc aagtaacgta taatactttt atatagtagg
    83881 aagaaagggg ataattaata tggaaagtaa tgattcttat ttagagtatg caaaagcaaa
    83941 taaggataag tttatttctt atattatcaa agggaaatcc cccgaagttg gacgagacgc
    84001 agtttttatg gcagggagtc ctggggctgg aaaatcagag gttgccattg gattagctga
    84061 gaattatgat aaccatgtga ttattgatgc ggattacttt cgcacccagt ttcctgatta
    84121 taatggaaaa aactctagtc tctttcaaaa agcatcttct tggttggtag agcaggcatt
    84181 tagatatgtc ttagatcatg gctattcttt tattctggat gcaacttttg caatattaag
    84241 tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt cgagtgacga ttttctatgt
    84301 ttatcaagac cctaaaatcg cttggaaatt tacacaagaa cgtgaaattg cagagggacg
    84361 tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa tcaagagaga acattgcaaa
    84421 agtaaagagt aaacatccag atgtattgct tcatatcata ataaaagatt atacgaataa
    84481 tatttccgaa gttcattatg ctacggataa tctagaatta tttttaccac tagggtatag
    84541 taggaaagat ttggaggttc aattacatga ctgagaaaga acaagtaacg aaaattgtaa
    84601 agaaatacaa taagagcatt gcggatttat cggaaaatgc tactgcaaaa gagttcaaaa
    84661 cggtgattaa atatgtagcc gatcaggcca atgaaaaaca acggaaatta gttggattaa
    84721 ataaaaaata gtaggacaaa ttaataatgt atgagccaag agcaaaatgt tcttggcttt
    84781 tttctttgca atgaatgagg taatgtatga gatttttaga tttattcgcc ggtattggtg
    84841 gctttcgttt aggtatggaa caggcgagcc atcattgtat cggtttttgt gaaattgata
    84901 aatttgctag acgtagttat aaagcaattc atgatacaag taaggaggtg gaaatgcatg
    84961 acatcacaag tgtatcagat gagtttattc aatccctcgg accagtggac atcctttgcg
    85021 gcggatttcc gtgccaagct ttttcaattg cgggaaagcg gcaaagattt tccgatactc
    85081 gaggtacttt attctttgaa atcgctcggt tcaccactct tctccgacct aagcttttat
    85141 tccttgagaa cgtccgagga ttgctcaatc acgaaggagg ggctacgttc gagacgatcc
    85201 tccgaacgct ggatggattg gggtatgacg tggagtggta ggtgcttaac tcaaaggcct
    85261 acattcccca aagccgggag cggatattcc ttatcggaca ttctcgagac gcatgtaccg
    85321 aacaagtatt tcctatcatt ggaccgtctt caacatctga tcaaaacatc agaaatttgg
    85381 taaatatcaa tccttccaat cgaggaatgg gtggacaagt ctatggttca gatggcgtag
    85441 cgccaacctt aacaggtgat gaaggaatca aaatcgcact gccagcaagg gatggaatct
    85501 ctgtagcagg actattaccg ggaaagtttg aacaaggtaa tcgagtttat gaagttactg
    85561 gaaccgcccc caccttgtca acgaaacaag gtggtacaaa aatcatgatt cgaaaaaatg
    85621 aaacagaata tcaagaggtg aagccagaag acagtgtgaa tctggctttt cctaattcca
    85681 caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac cttactaaca ggagaccaac
    85741 aagcgatcgt cacggatcag tatcaaattc ggaagctaac acctagagag tgttggcgac
    85801 ttcagggctt tcccgattgg gcttttgatc gagattctca agtcaattca gatagtcagt
    85861 tgtataagca agccggaaac tctgtaacag ttccggtcat ttttgatatt gccagaagat
    85921 taaaagagtt ggatgtacat ggtttatgat gagttgattg gagaaattta ttgggtgatt
    85981 ggaaagattc agtcagaccc agaattagaa gaacaattgc gacggttaaa atttgatatt
    86041 cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa tgaatgagga gacggatgct
    86101 cgaattgaaa taaatcaagt aattgcagaa tttgagcgga ttgcggacca agcaaaagaa
    86161 ccagatattc gtcaatactt gttagaaatg aaagcagagt tggaagtaaa tggtattaca
    86221 gatgagtaac ctaaaccaac acaactgact gttaggcgct gaccgcggag gattcgcgaa
    86281 gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc gttagccgac catgatgtct
    86341 cacccccgta tctaacaggg gggtacaaaa acacaaaaaa taagtaagca accattaaga
    86401 atgcttgttg tatcaaagat agtaagtaat ttgtataggt gttaactcat ttgaaaaagt
    86461 taacaccttg aatttggagg tgagaacttg gcacaacgga atttagatta tcggctatta
    86521 aaagaccgac gcaacgaata cggcgtttca caaaataaat tggctacggc ttgtggactt
    86581 agtcggccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatctat aaaaaccatg
    86641 aggaaaattt ttgaccaact ggaaagcttt aatcccgatt tgcctttgac gttactgttt
    86701 gactatgtaa ggattcgctt tcccacaacg gatgcacgaa aaattattca agaaattctc
    86761 catttgaaat ttgagtatat gctccatgaa gattatgcct tttactccta tcaagaacag
    86821 tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag aagataaagg tgtgctttta
    86881 gaattaaagg ggcgaggttg ccgacaattt gaaacttttt tactcgctca aaagcgtagc
    86941 tggtacgact ttttcgagga ttgtctaaag gccggtggcg tgatgaaacg cttggacttg
    87001 gcaatcaatg atcgagtggg gctattggat atacctgagc taacgaaaaa atgtcagaag
    87061 gaagaatgta tctccttgtt tcgtaccttt aaaagttatc gttccgggga acttttaaag
    87121 gctgatgaaa aggacggcat gggcaatacc ttatatatcg gcagtctcaa gagtgaggtt
    87181 tatttctgtt tgtatgaaaa agactatgaa caatatatca agttagggat tccattagac
    87241 caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa atgaccgtgc ttttcatgcg
    87301 attcaagatt tgttaaaagg tcgtagcatt gagagcacca cgttttccat tatcaatcgt
    87361 tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa ccaactggcc cttgaatgaa
    87421 caatgggggc gttttattgg acggaatcgg aaagagattc aattaacttc cgagccaaaa
    87481 ccgtatacca ttgaacgaac cttgaattgg ctaggccgac aagtcgcccc tacgtggaaa
    87541 atggctaagg agttggatcg attgaatcgg acaacctata tccaggatat ggtacagaat
    87601 gcacgcttat cagaccgaca taaaaagatt ttggagcaac aaagtatggc aattgaaaat
    87661 ttgattatat gaaaagagga atcgtcatgg gaattgtaaa agatattttt ttggtatttg
    87721 gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa tgcaagtgca gcagcggatc
    87781 gagcaatgga aaagaaagga aagtgacaag atgaatttcg gacaaaattt
//
LOCUS       DAPBCS010000004        84113 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPBCS010000004 DAPBCS010000000
VERSION     DAPBCS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 84113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.004"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            219..1277
                     /locus_tag="RD521_000373"
     CDS             219..1277
                     /locus_tag="RD521_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDT7770136.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1281..2315
                     /locus_tag="RD521_000374"
     CDS             1281..2315
                     /locus_tag="RD521_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDT7770137.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2417..3133)
                     /locus_tag="RD521_000375"
     CDS             complement(2417..3133)
                     /locus_tag="RD521_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7770138.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3102..3887)
                     /locus_tag="RD521_000376"
     CDS             complement(3102..3887)
                     /locus_tag="RD521_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDT7770139.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3878..4627)
                     /locus_tag="RD521_000377"
     CDS             complement(3878..4627)
                     /locus_tag="RD521_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDT7770140.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4925..5449
                     /locus_tag="RD521_000378"
     CDS             4925..5449
                     /locus_tag="RD521_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDT7770141.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5465..6964
                     /locus_tag="RD521_000379"
     CDS             5465..6964
                     /locus_tag="RD521_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDT7770142.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7052..7654)
                     /locus_tag="RD521_000380"
     CDS             complement(7052..7654)
                     /locus_tag="RD521_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7770143.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7758..8714
                     /locus_tag="RD521_000381"
     CDS             7758..8714
                     /locus_tag="RD521_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDT7770144.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(9019..9975)
                     /locus_tag="RD521_000382"
     CDS             complement(9019..9975)
                     /locus_tag="RD521_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7770145.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10149..10709
                     /locus_tag="RD521_000383"
     CDS             10149..10709
                     /locus_tag="RD521_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770146.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10702..11526
                     /locus_tag="RD521_000384"
     CDS             10702..11526
                     /locus_tag="RD521_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDT7770147.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11536..12153
                     /locus_tag="RD521_000385"
     CDS             11536..12153
                     /locus_tag="RD521_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7770148.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12171..12854
                     /locus_tag="RD521_000386"
     CDS             12171..12854
                     /locus_tag="RD521_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDT7770149.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13537..14820
                     /locus_tag="RD521_000387"
     CDS             13537..14820
                     /locus_tag="RD521_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7770150.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVNKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14836..15723
                     /locus_tag="RD521_000388"
     CDS             14836..15723
                     /locus_tag="RD521_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7770151.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15740..16576
                     /locus_tag="RD521_000389"
     CDS             15740..16576
                     /locus_tag="RD521_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7770152.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16579..17202
                     /locus_tag="RD521_000390"
     CDS             16579..17202
                     /locus_tag="RD521_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770153.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17199..18491
                     /locus_tag="RD521_000391"
     CDS             17199..18491
                     /locus_tag="RD521_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7770154.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18850..18947
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19063..19644
                     /locus_tag="RD521_000392"
     CDS             19063..19644
                     /locus_tag="RD521_000392"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDT7770155.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19647..20954
                     /locus_tag="RD521_000393"
     CDS             19647..20954
                     /locus_tag="RD521_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDT7770156.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMILISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21370..21858
                     /gene="purE"
                     /locus_tag="RD521_000394"
     CDS             21370..21858
                     /gene="purE"
                     /locus_tag="RD521_000394"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDT7770157.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21851..22975
                     /gene="purK"
                     /locus_tag="RD521_000395"
     CDS             21851..22975
                     /gene="purK"
                     /locus_tag="RD521_000395"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDT7770158.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22988..24283
                     /gene="purB"
                     /locus_tag="RD521_000396"
     CDS             22988..24283
                     /gene="purB"
                     /locus_tag="RD521_000396"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDT7770159.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24463..24840)
                     /locus_tag="RD521_000397"
     CDS             complement(24463..24840)
                     /locus_tag="RD521_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770160.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            25096..25824
                     /locus_tag="RD521_000398"
     CDS             25096..25824
                     /locus_tag="RD521_000398"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDT7770161.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25824..26078
                     /gene="purS"
                     /locus_tag="RD521_000399"
     CDS             25824..26078
                     /gene="purS"
                     /locus_tag="RD521_000399"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDT7770162.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            26080..26751
                     /gene="purQ"
                     /locus_tag="RD521_000400"
     CDS             26080..26751
                     /gene="purQ"
                     /locus_tag="RD521_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDT7770163.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26752..28974
                     /gene="purL"
                     /locus_tag="RD521_000401"
     CDS             26752..28974
                     /gene="purL"
                     /locus_tag="RD521_000401"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDT7770164.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28959..30398
                     /locus_tag="RD521_000402"
     CDS             28959..30398
                     /locus_tag="RD521_000402"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDT7770165.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30421..31473
                     /locus_tag="RD521_000403"
     CDS             30421..31473
                     /locus_tag="RD521_000403"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDT7770166.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31470..32048
                     /gene="purN"
                     /locus_tag="RD521_000404"
     CDS             31470..32048
                     /gene="purN"
                     /locus_tag="RD521_000404"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDT7770167.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            32045..33580
                     /gene="purH"
                     /locus_tag="RD521_000405"
     CDS             32045..33580
                     /gene="purH"
                     /locus_tag="RD521_000405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDT7770168.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33596..34870
                     /gene="purD"
                     /locus_tag="RD521_000406"
     CDS             33596..34870
                     /gene="purD"
                     /locus_tag="RD521_000406"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDT7770169.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34992..35906
                     /locus_tag="RD521_000407"
     CDS             34992..35906
                     /locus_tag="RD521_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7770170.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(36039..37604)
                     /locus_tag="RD521_000408"
     CDS             complement(36039..37604)
                     /locus_tag="RD521_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7770171.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37597..39048)
                     /locus_tag="RD521_000409"
     CDS             complement(37597..39048)
                     /locus_tag="RD521_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7770172.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(39062..40078)
                     /locus_tag="RD521_000410"
     CDS             complement(39062..40078)
                     /locus_tag="RD521_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7770173.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            40298..41386
                     /locus_tag="RD521_000411"
     CDS             40298..41386
                     /locus_tag="RD521_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDT7770174.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41440..42966
                     /locus_tag="RD521_000412"
     CDS             41440..42966
                     /locus_tag="RD521_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDT7770175.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42968..44173
                     /locus_tag="RD521_000413"
     CDS             42968..44173
                     /locus_tag="RD521_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7770176.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44430..45407
                     /gene="guaC"
                     /locus_tag="RD521_000414"
     CDS             44430..45407
                     /gene="guaC"
                     /locus_tag="RD521_000414"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDT7770177.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45671..47095
                     /locus_tag="RD521_000415"
     CDS             45671..47095
                     /locus_tag="RD521_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDT7770178.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47556..48665
                     /gene="pdhA"
                     /locus_tag="RD521_000416"
     CDS             47556..48665
                     /gene="pdhA"
                     /locus_tag="RD521_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDT7770179.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48668..49645
                     /locus_tag="RD521_000417"
     CDS             48668..49645
                     /locus_tag="RD521_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDT7770180.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            49670..51313
                     /locus_tag="RD521_000418"
     CDS             49670..51313
                     /locus_tag="RD521_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDT7770181.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            51321..52727
                     /gene="lpdA"
                     /locus_tag="RD521_000419"
     CDS             51321..52727
                     /gene="lpdA"
                     /locus_tag="RD521_000419"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDT7770182.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            52889..53164
                     /locus_tag="RD521_000420"
     CDS             52889..53164
                     /locus_tag="RD521_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDT7770183.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            53170..53937
                     /locus_tag="RD521_000421"
     CDS             53170..53937
                     /locus_tag="RD521_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDT7770184.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            54105..55934
                     /gene="typA"
                     /locus_tag="RD521_000422"
     CDS             54105..55934
                     /gene="typA"
                     /locus_tag="RD521_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDT7770185.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            56195..57967
                     /gene="recQ"
                     /locus_tag="RD521_000423"
     CDS             56195..57967
                     /gene="recQ"
                     /locus_tag="RD521_000423"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDT7770186.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            58081..58383
                     /locus_tag="RD521_000424"
     CDS             58081..58383
                     /locus_tag="RD521_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDT7770187.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            58386..59549
                     /locus_tag="RD521_000425"
     CDS             58386..59549
                     /locus_tag="RD521_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDT7770188.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKNFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            59575..63003
                     /locus_tag="RD521_000426"
     CDS             59575..63003
                     /locus_tag="RD521_000426"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDT7770189.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            63024..64148
                     /locus_tag="RD521_000427"
     CDS             63024..64148
                     /locus_tag="RD521_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770190.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            64138..64575
                     /locus_tag="RD521_000428"
     CDS             64138..64575
                     /locus_tag="RD521_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7770191.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRVVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            64559..64897
                     /locus_tag="RD521_000429"
     CDS             64559..64897
                     /locus_tag="RD521_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDT7770192.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            65016..65576
                     /gene="rsmD"
                     /locus_tag="RD521_000430"
     CDS             65016..65576
                     /gene="rsmD"
                     /locus_tag="RD521_000430"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDT7770193.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            65573..66064
                     /gene="coaD"
                     /locus_tag="RD521_000431"
     CDS             65573..66064
                     /gene="coaD"
                     /locus_tag="RD521_000431"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDT7770194.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            66054..67097
                     /locus_tag="RD521_000432"
     CDS             66054..67097
                     /locus_tag="RD521_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDT7770195.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            67256..67984
                     /locus_tag="RD521_000433"
     CDS             67256..67984
                     /locus_tag="RD521_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDT7770196.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            68100..68345
                     /locus_tag="RD521_000434"
     CDS             68100..68345
                     /locus_tag="RD521_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7770197.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            68342..68560
                     /locus_tag="RD521_000435"
     CDS             68342..68560
                     /locus_tag="RD521_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDT7770198.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            68696..69235
                     /locus_tag="RD521_000436"
     CDS             68696..69235
                     /locus_tag="RD521_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDT7770199.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            69228..71501
                     /locus_tag="RD521_000437"
     CDS             69228..71501
                     /locus_tag="RD521_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDT7770200.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            71539..72585
                     /gene="holA"
                     /locus_tag="RD521_000438"
     CDS             71539..72585
                     /gene="holA"
                     /locus_tag="RD521_000438"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDT7770201.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(72674..72928)
                     /locus_tag="RD521_000439"
     CDS             complement(72674..72928)
                     /locus_tag="RD521_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDT7770202.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(73328..73597)
                     /locus_tag="RD521_000440"
     CDS             complement(73328..73597)
                     /locus_tag="RD521_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770203.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(74067..74522)
                     /locus_tag="RD521_000441"
     CDS             complement(74067..74522)
                     /locus_tag="RD521_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDT7770204.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(74660..76588)
                     /locus_tag="RD521_000442"
     CDS             complement(74660..76588)
                     /locus_tag="RD521_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDT7770205.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(76785..77618)
                     /locus_tag="RD521_000443"
     CDS             complement(76785..77618)
                     /locus_tag="RD521_000443"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDT7770206.1"
                     /translation="MFFANIIKAVILGIIEGITEWVPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(77810..78514)
                     /locus_tag="RD521_000444"
     CDS             complement(77810..78514)
                     /locus_tag="RD521_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7770207.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(78537..79163)
                     /locus_tag="RD521_000445"
     CDS             complement(78537..79163)
                     /locus_tag="RD521_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7770208.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            79489..81864
                     /locus_tag="RD521_000446"
     CDS             79489..81864
                     /locus_tag="RD521_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDT7770209.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(81888..82379)
                     /locus_tag="RD521_000447"
     CDS             complement(81888..82379)
                     /locus_tag="RD521_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDT7770210.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            82566..83486
                     /locus_tag="RD521_000448"
     CDS             82566..83486
                     /locus_tag="RD521_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7770211.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            83640..83984
                     /locus_tag="RD521_000449"
     CDS             83640..83984
                     /locus_tag="RD521_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDT7770212.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
BASE COUNT        27278 a        14565 c        18080 g        24190 t
ORIGIN      
        1 taatttaagt taaccttaaa actaaacttc ttaagtttgg ttttaaggtt ttttttgtgt
       61 tctagtaaaa atcactttac ataagtttca aaagtttctt taatttccta ttgttcaata
      121 agtaacaatg taaagataaa attaaaaatg ttaatacgta ttagcaaaat aaaagctttt
      181 tttattgtga ataaatgaac ataattagga ggagaaagat gaacttattt gacatttttc
      241 gatcgacttt gacagatatg tcgattgtca gtgcaatcac ttcaacagtt tttattatat
      301 tattagggtt tgtttgtaga aaaaagggga ttttttctga atctgttgga aaaattctat
      361 cgaaagtcgt cttaacagtg gctttgccag cactggcatt tcgagctttc atgcaagata
      421 tcaatacaga attactgatg caaggaatca atgtcttgat ttggggatta gttatttata
      481 ttattcttat tctagtttca aaaccttttt tcttaagcta taaaggggat aagcaaacaa
      541 cgttgcgtgt cctgacgatc tttggttcta cgaccttttt cggaacgcct atcgtcagtg
      601 cgatttatgg tcctgtcggc gtgatgtatt cctcaatttt caatattggc taccgaatct
      661 ttttgtattc ttatggatac ataaaaatga gtggcttgaa gatggaagct aaaaatatca
      721 aagcgatgtt cgtcaatcca atcattatcg ctacttttgc cggccttttc atttggctgt
      781 tccaagggta tctgccacaa gtgtcagtta caggtgcaga aggagagata agccaagtag
      841 cgtttttgcg aattgaccag actgcaccgt ggctatttca gccgatggat tatttagctg
      901 gtttaagttc accattggcg tggctagcaa tcggcgcaac attaggtgaa gtaagcttca
      961 aaaaagctgc atctgaaaaa acttcatggt attacagtct agtcaaagtg gtatttgtac
     1021 ctgcattgaa tatcgtgatt ctagcagttt tgacggcaac gaatattcta ccagtcaatt
     1081 ttgaagcact ggcaacaatc gttatcatga tggctacacc agctgcaaca gtggcagcag
     1141 cctatgctat cagttttgat agggaggcac ttttagcctc caatgcatcc ttactgtcaa
     1201 ctatttttgc tgtggtactg acacctgtat ggatcgttgt attacaaatg attcatggca
     1261 taggtttatt ttcataagaa atggaggaag aaagtatgag aatcgcatgt tacggtgttc
     1321 gtccgaacga gatagatttt tttgaaaaat tgaatattta ccattatgat ctcagtcttt
     1381 atgaagaact gttgacacat gacaatatcg tgtcagcaaa ggaccatgat gctgtattgt
     1441 tgaggggaaa ttgtatagca gatgagacca atcttgcaaa aatgcaggaa tatgggattc
     1501 gttatgtttt tacacggact gtgggagtca atcatatcga tctacaagcg gcagctgatt
     1561 ttggtatgac agtagcccgt gtaccatcgt attcgccaaa tgcgatagca gagttatctt
     1621 tgacttttgc catgatgctt ttgagaaata ctgcgtatac gacgatccga acatctttca
     1681 aagattttcg agtggatgag caaatgttta gtcgtgaaat cagaaattgc acagtaggga
     1741 tcataggaac aggcaggata gggttgacgg aagctgtttt gtttaaggga ctaggagcca
     1801 aagtgatcgg ctacgatatt catcagacag aagctgctaa aaaagtattg acgttccact
     1861 cgcttgaaga gttattagcc aaaagcgata ttgtcagtat tcatgtacct catatcccag
     1921 gtgaaaacga tcaaatgatc aatgaaggat ttttagctca aatgaaaaaa ggtagcatct
     1981 tgattaatac agcccgtgga gagcttcaag ataatgaagc tattttaaga gctttgaaaa
     2041 gcaatcattt agctggtttt gcaaccgatg tttttgcaaa tgaaacagag gtttttttcc
     2101 gatccttcaa gccatgggag acgattccag accctgctat ccagcaatta gtagaaatgt
     2161 accctagagt tcttgtaacc ccgcatgtag gttcaaatac agatgaggca ttatcaaata
     2221 tgatttcgac aagtttcgag aactttcgtg aaataataga aactggaaaa acgaaaaatg
     2281 aggtatctct tcctaaagcc cgtcaattaa gataagaaaa catggatatt cgtcaataaa
     2341 atgtttaaaa atagtccaag taaacagttg ttacttctgt atacttggac tattttgtat
     2401 gggataattc gatgactcaa cagtaatctg tgagcttttt ccgcccattc tttgtcaatt
     2461 cataacaggt tagcggccgg ccgatttcca aatatttgat tctttcgttc aatatatctt
     2521 gttctactaa aaaattcaaa tatttcttga cggatatacg tgacagtgag cttttttgag
     2581 ccagttcttt tgttgaaaaa atagtatctt cttttagaat aaatgcactt atttttttta
     2641 aagtcagttt tgacagacct ttaggcaaat cgatgaatga tggtatttcg tgaatgggag
     2701 tggtgtggtt gaatacttga tcgatcaatt tctgatcggc tttttcagtc gtttggattg
     2761 tctgagagaa agcaagatac ttttcgatcg ccagctgaaa acgttgcaaa gtgaacggtt
     2821 tgatcaaata atctaaggca ccgtagcgga tcacattctt tatggtttcg atatcatctg
     2881 cagcagtgat caaaatcact gggatatcat ttccttgctg tctaagccaa gataaaaaat
     2941 ggataccact ttcgccaggc atgtatacat caagcagcag cagatctgcg tttttttgtc
     3001 tgaaatattt ttttccttct tctacatttc tgaaactttc gattacgttc atattaggga
     3061 tgctttccac aaaagtacga ttcaattctg caaccattgg atcatcttcg attatcagga
     3121 catttgtcat cattttgatc ttcctttttt ggtaattcta gatagacgat tgtaccgcca
     3181 tttgtttgtt tggaaaattc taaaaatcca ccgtggcgtt tgacgattga atggatgaga
     3241 ttcaatccaa acccacgatt gattcccttc gtcgaaaaac ctagcgtaaa tattttttct
     3301 gagatttctt ctgatatccc gacaccgtca tcgcagacgg tcacgatgaa tacttcagct
     3361 tctgtgtcat agtttaaatg aatccggacc tgtccgtttc tttcatgttt ttgtagcacg
     3421 gcttcttttg cattatcaag taagttccct aacacttgca aaagtgaatg gatggagtca
     3481 tccatctcca ttttagggat aaaagaatcg ctttctaaca gcatttcaac agattgttct
     3541 ttggcttcgt ttattttgcc taataaaaaa ccggcaattg caggtacttt gatcagactt
     3601 gagacttggc caatctcttt tttataattt tgatttagct gctggataaa atgggtgact
     3661 tcttcatact tcttcaaatc aatcaaacca gaaatgacat gaagtttatt catgaattca
     3721 tgggtttgag ctctcatgga gtttatatat tgtttcgtcc ccccaagctc ctccattaat
     3781 ttcattactt ctgatcgatt tcgtaaagtt gcgactgctc ctaaaaaacg gtcattgcta
     3841 aaaagaggtg taacgctaga aatagtttct atcccattta aatacatgga ttgagtccga
     3901 acggtcgttt gatgctcaat cgtctcttta aacaaaacgt catatagatt atcactgatt
     3961 tttttcctac ttcgaaatcc aatagctgct ctgtttggta gaaaatgttt tttgcaggtg
     4021 tattgatcaa gaggatagtt ttatcagaag agatggctaa gattccttca tctacttcat
     4081 tttcgataat cgttttttca cttaactgtt ctgctatttc ttgtggttca agtccaagca
     4141 gtactttttt cattttttgg gcaagaaaaa cggcgcctat gatgcctacg ctaagtccaa
     4201 taatcaatga aaaaacaatc attttttgtc catctttgac ttgattgtca atcgtgttgt
     4261 ttgttaagcc aacacaaact gcaccaataa tctcttgatc ataataaata gggacgaaat
     4321 agcggaagcc gaaccctaac attccttctt tttcagagaa aaattcttgt cctccaatca
     4381 cttttttcac atcttcatga cttgagaaag gaagaccgat gtaacttggg tctgcgtgag
     4441 aatatcgaat gccttcaggg tccatgacaa cgataaaatc taaattcgat gcaatcatga
     4501 tttcatttgt tattccttga atcgcatgtg tcgggtcacc agtctgcagc tttttttgga
     4561 tgagtttatc attggcaacg attttagcaa tgctagagat tttatctgac acactagatc
     4621 ggttcatcga aagaataaaa ttcctgatca atatggcaga aacaatcaaa gaaacgaatg
     4681 tggagaaaca aacgacaagg atgatcgtgt tctggagctt taatggtttt ttcttaaaaa
     4741 aaggaaacat aggctacctc gaaagaattt attgtgaaaa ttttaacatg aaaacaaaaa
     4801 tttaacaaat tgtcaacgat aaacacaacc ttcctataaa aaacaccact gagtcggtaa
     4861 aagaagctat acagaatttt caaacattgg tatgatagac gtagaaatgg aaggaggcgt
     4921 gtatatgata gggggaatcg cacttgagac gatttattgg tatacattgc tgatttgtgc
     4981 aggattggca gtcctattga ttatttttgg agatgtattc gattttgatg gcccgcttga
     5041 tccgatgctt attatccctt ggttaacttt cacaagctta tttggttttc taggtgagcg
     5101 tttaacagat caatcatctt tatttttatt tattgctagt ggaggcatat cgatagtgtt
     5161 ggtatttttg cttaatttct atatattgat gccgatgaaa catgccgaat caacgatgtc
     5221 gtcttctgaa aaaactctgg aaggacaagt agctgttgtg atcacaccga tccctgtacg
     5281 aggtatggga gaaattcagt tgaaaagtgt taccggttcc attagtcgtc cagcttgttt
     5341 ttacagcccc caagaacaga cagctgagcg gggaacgaaa gtattgatca ttgagatcaa
     5401 ggaaagaatc tgttatgtca tgccttatga aagcatgata aaagcttaaa ggagcgtgtg
     5461 ttaaatggaa ataccagttg tttttatcgc gatcgcaatc gcagctttga ttttactgat
     5521 gttgctgatc gtctttgtag cgaagtacca gacagcaaaa ccggatgaag cgttgatcat
     5581 cagtggaagt tatttaggaa gcaaaaatgt gcacattgat gaaagcaata acaaaatcaa
     5641 aattgtgcgt ggaggtggcg cattcgtctt gccagttttt caacgttcga atcggatcag
     5701 tctattatcg agtaaattgg acgtctccac ccctgaagtg tatacggaac aaggcgtacc
     5761 ggtgatgtgt gatggtactt cgatcatcaa aatcggttca tctgtagaag agattgctac
     5821 agcagcagaa cagtttttag gcaagacaag agaagaactg gaaaatgaag caagagaagt
     5881 attagaagga cacttgcgct cgattcttgg ttctatgact gtagaagaaa tctatcaaaa
     5941 tcgcgataag ttcagtcaaa gcgtacaaga agtcgcttct gtagaccttg caaaaatggg
     6001 gttgatcatc gtttctttca ctatcaaaga ggtcagagac aaaaacggtt atcttgactc
     6061 tttagggaaa ccaagaatcg cccaagtcaa aagagatgca gatatagcag aagcggaagc
     6121 tctaaaagaa acgcgtatca aaaaagctca agcagaaaaa gaatcacaag cagctgaatt
     6181 acagcgacaa acagagatcg cagaatcatt aaaagaaaaa gaactgaaat tagcaaccta
     6241 caaacaagaa caagatgttg ctaaagcaaa agcagaccaa gcctacaatt tagaaagtgc
     6301 ccgagcacaa caacaagtaa tcgaacagga aatgcagatt aaaatcgtag aacgtcaaaa
     6361 acaaatcgaa ctggaagaaa aagaaatcac acgtcgtgaa aagcaatatg attcggaagt
     6421 gaagaaaaaa gcagatgccg atcggtacgc taaagaacaa gaagcacaag ctcaaaaagt
     6481 caaagaagtg actgaagccg aagcagaacg attccgtgtg gaagcactgg cagaagcgga
     6541 agccaataag acacgtttag ctggacaagc agaagccgaa gcagcactag caaaaggaaa
     6601 agcagaagcc gaagcaaaac aaaagatcgc caatgccttc aaggaatatg gcgaagcagc
     6661 tgtcttgagt atggttatcg atatgctacc gcaattgatg cgcgaagcag cacagccgct
     6721 aggaaatatc gaaaaaatct ctgttgtcga tacaggaagc agttcaggag aaaccagtgg
     6781 agcgaaccga gtgacaaatt atgccacaaa tttgctttct tctactcaag aaacactaaa
     6841 agcgacaaca ggacttgact tgaaggaaat cgtagagaat ttgtcgacac gtgggactac
     6901 tagccaagta aatatgtatg aagcgccaaa agaggcagaa caaaactcga tgaaggaaga
     6961 ataaagtagt acacttcacc gttcaatcaa aaatggagga agacttttta aagttcttct
     7021 ctccattttt ttattttatt gcaaaagaaa attagtcctc gctatttttc ttttgttttt
     7081 ggatcttagc ttctatctct tgtaaatttg ttcttagcaa tcgttctttc ttttggagtt
     7141 cgaccaaatc ttctgatagt tcttccttct tttctgtttc aggaggacga agaaagaaat
     7201 tgataccgat tgcaatgaat gtccctataa aagtatctag tacacgttgg atagcataca
     7261 aagcggattc gccttgaggg atgctcaacg caatcagcaa caacgtagct attgcggaaa
     7321 tgataccgga attattgtta ataccatctg acacgacgat cacaataata actagaatag
     7381 gaagcaagat caattctacc aaaaaatcat tgtgaaaata gcttttgaca aaaaaataaa
     7441 tgatagctaa tgtaccacct aatgtattgc cgatgatcct agactttcca aaagaaacgg
     7501 ttgtagtcaa gtcctgacgc aaagaaaaaa cagcagataa tgcagcaatc atcgggattc
     7561 ctcgttgagt caaatgaaaa aacaagatac ataaaaaaac agctaaggca cttttgatcg
     7621 tacgcatacc tagtcgaaaa cggccaaatt gcatagagat tctccttttt ggtcttttta
     7681 ctatcatacc taaacttttg tctgatagct agtttttcat tattcaagta taattaaaat
     7741 aaaaaagggg gaagtaaatg cgtataggaa tgcgaacagt caaaacagtg atcagtgtct
     7801 tcttttcgat ggtcacagcg ggtagcttat cgctgttgta ttggccagct gcagggatca
     7861 ttgcattatt aagtgtagga aataccaagc gctctacatt gcgtacgggc atgaatcgaa
     7921 tcgcagcctt tatattagct acggcaacag cactgttcag tttttttgct gttgggtata
     7981 ctatttttgg cttcagtctt tttttattcc tgttcatccc gcttgcctct cgcttcaaat
     8041 taaccgaagg gatagtagta aattcggttt tggtcactca ttatttagtg gaggaaaaca
     8101 tgtcttggca gctgatcggc aatgaagcgg gattaatggc gattggatta gtatttgctt
     8161 tgcttgcaaa tgtttacatg ccagatcgta cgaagcagct gaaggaaaac caaatacaga
     8221 tcgaaaaaga atttcgaaat attttgagac aaatggcaga atttctttta tcagaaaata
     8281 aagaagatgt gcagatcaaa tgcgaacatt tactgacatt cattcgcgaa agccaagaag
     8341 atgccagaga acatcaagaa aactactggc tcagacagcc gctatactac gaaacttact
     8401 tctctatgag aagagcacag gccaatgtga ttaaagacat gctggaaaat ctagagagaa
     8461 tccagcaacc agcttattat ggaaaacata tctatggttt attgatttat actgcagaaa
     8521 cattttcaga gtcaaatgat ggcagacaaa ttttaacacg tattgaagaa gtttatgtat
     8581 tgtatcggca aatgcctctg ccgactagtc gccctgaatt cgaggatcga gcagaattgt
     8641 tccagttttt gcagtctttt aaaagtttta ttgaaatcaa ggcagaattc tcccaacaaa
     8701 aaatccaaaa atagaggttg tgaattcagc taatattcag gtcaaaaagc tgatttcaca
     8761 acctcttctt taaacggtgt tctcctgtca actcctcggt cacaagtcac aatattcaac
     8821 aaacatgaga agctgttttt tgaaattgct ccttgtgact cggagctgaa cggtaggctc
     8881 acaacctctt tcttaaacgg tgttcaaaag ctttcctctg cggtaataag tccagccaaa
     8941 cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt
     9001 tttcacaacc ttttaaaatt acatattttt ccatagaact tgcatttgat ccaagatttc
     9061 gatgacatct tttgtcaaga agagagagcg attgggcaag tcaccttcaa tcatgtgtac
     9121 catattcttt atttcgtaat tcattgcttc agatgtattc ccgctttcga tgaattcctt
     9181 tgtaccatct gtaaataaga tctccgcacg atcagcacgc gggtaatcag caatcgtcac
     9241 ataaccgttc tcaaaggcaa cgataccgac ttttggcatt tttgcctgaa aagtcaagct
     9301 gacagcagca agttcatttt ctttgttacg caagatagtg acggattgtt cgtccacgcc
     9361 tgtttcaaaa ggaaccattg tagaggcaac tacttcaggt tgagagctca aaaagaaacg
     9421 agcgaaagat acagcatacg taccgatatc caacagagct cctccagcta attctggatt
     9481 gaagaaccgg tttttagggt ctggttcttt gtaactgccg aaaggtgctt ggatcatttt
     9541 taaagccccc agttttcctg tatccattat tgagcgtaac tgctgataaa gtggcatgtt
     9601 gaagattgtc atcgcttctg ctaagatcaa attcttttct tctgctaagc gcatagcgtc
     9661 agccagttct tttttattca tcgtgatggc tttttcacat agaacgtgtt tgcctgcttc
     9721 caatgctgcc aagatatggt cgatatgctg tctatttggt acagcaatgt acacgatgtc
     9781 gatttcagca tcgtctaaca tttcttgata gcttccgtaa gcttttggaa tattgtaacg
     9841 gtgagcgaag ttttccgctt tttctgaagt tctggaagca actgctgtta gttcgctcgt
     9901 ctcttgattg aagctttcgg caaactcatg tgcgatggta ccaagaccaa cgattcccca
     9961 ttgatatttt ttcatctgaa aacgctcctt catagttgga ttgagtttct tgtctgtcta
    10021 ttattataac aaaaatcacg aagaataaag aggagaggac ttgaaacatt aaaaaaaatg
    10081 aaagcagcga tttaagaggt gctttttgat caaaacttaa gtagaatagt taaaaggagg
    10141 ggataaacgt gcatcaagta tatattgatc ttattatgag tgtattatta gaacagtttg
    10201 aaactgaaaa aaatttttta gatgaatact tgctgatcag tccagaacaa tggcaaaaat
    10261 ggaaagatgg cgagaaaaat ctgccatctg aagcgatgca gaaagtaaaa aatcttttca
    10321 ctgattatga atggatgcta acgcaaaaga ttttgcgtca aacgattatt tttcccgaaa
    10381 aacgaaacgt tgccgttgca gagtacaagc aaatcaaaac acgcatcgcg caaaaatggc
    10441 ttagcagcaa agcagcaaaa gtcgagctga tccctttgaa aaataatcag caggctggta
    10501 gataccttga cttgaaagta agtattcaat atgatgaatg gggctatgat gatattttga
    10561 attttcgtct gccagcagat gttcaaaaac agatcgaagg agaaaaaatc gaacttctcg
    10621 attgggtgaa tgaaaatctg gaagaaactt acgttcatga ggcgaatggg gaacgtgaag
    10681 aacaacgaaa ggatttttca agtgaataaa tttttaggat ttggagcagt tgtgattatg
    10741 atcgcagcaa tgggctatgt tatttttagc gatgatgatt cgcatgttca agccggcaat
    10801 caagcagtcg aacgaacaag caccaaaaca actgctagta aagaaaagaa cagtcaaaca
    10861 accagcagca aggtaaagga caaagaaaca aaagatcgta cgtccaaaca attgccagat
    10921 gttaaaacag acgattggaa gctagttttg gttggtccag atcacaaatt agaaaaagaa
    10981 attgatgagt ctactcaatt ggcatcatta agtaacggat acttgatcga caaacgcatt
    11041 gctccagaat atgaagcatt tgccaaggct gctgaagcag ctggatttcc attagtgatg
    11101 gtctcagctt accggtctgt atcatcccaa caacaagttt tcgaacaaaa tgtccaagat
    11161 gtgatgagtc gtcaaggtgt atcagaagag gaggcgacaa agattactaa agaaacgatt
    11221 acggagccag gatacagcga acaccacaca ggtctggcag tcgatgtcgt cgatcaaaac
    11281 tggtacaaca gttatccttc tactgtattg gatgcaagtt acggagatca gcctggagct
    11341 aaatggatcg cagataatgc tgcgcagtat ggatttatcg ttcgttatcc aaaaggacac
    11401 gaagatataa ctaagatcac atatgaacct tggcacttac gttatgtcgg taaagaaaac
    11461 gctgagtata tcaccaaaca ccaattgacg ctagaagaat atctgaatga attaaatgaa
    11521 aagtaggaat tgatcatggc gaaaaaaaag tggaaaaagc aaaagcgcaa gaatatccgt
    11581 tggccagcag tggttgccgg tgtagcagtg atactcttag cctttgtttt ttccttgaag
    11641 aatctgtctt ctccttttac tgataatcag caagaaaatg aagcgcaatc ccatcagcaa
    11701 tttattgaac ggcttgttcc gcatgcccaa gaactacaag atggttatgg agtgcttcct
    11761 agtatcattt taggacaagc tatattagaa tcaaattggg gaaaaagtac cttggctagc
    11821 aaatacaata atcttttcgg gatcaaggct tatggggatc agaagaaagt aagcctggaa
    11881 acaaaggaat ttgttaacga agaatggata actatccaag gagacttcaa agtgtatgat
    11941 tcatgggaac agtccatgga tgaccataca caactgttcg tccaaggagt agattggaat
    12001 cctgcgctgt atgaaaaagt gattacggcc accaactatc aggaagccgc acaagcattg
    12061 caagatgcag gttatgcgac agaccccggt tatgcgcaaa aaatcatcca ggtcatcgag
    12121 acgtatcaat tgaatcagta tgaccggaga tagcaaagga gaagtggctg atggcagaac
    12181 aatttacgcc agaaatcatg acggaattga gaaaagatat cgtaagagta ccggaaatca
    12241 ttttagaagc aagcgggatc aagatatttg gaaaattgat ccgttcgatc atctttacta
    12301 cggatattgc gatcatacgt aatacaaatg cgaatgcagt aattgctgtg tatcctttta
    12361 cacctcatcc agcgattact aaaagcatta ttgaggcagc tgacattcca gttttctccg
    12421 gtgtaggtgg tggtttgaca caaggtgctc gttcttccta catgagcatg ttcgcagaag
    12481 cgcaaggttc gttaggggtc gttttgaacg gtcctacgcc tttagagaca gtaaaagaag
    12541 tttgtcgagt agttgatatt ccagttatca gtacggtaac gtcgaaatat acagaaatag
    12601 atgaaaagtt agcagctggt atttcgatta tcaatatcag tgcaggtaaa gaaactgcag
    12661 aagcagtacg ccattttcgt aaaaaatatc ctgaacttcc tatcattgcg acaggtggtc
    12721 caacggaaga aagtattgct gagaccattc aagcaggagc aaatgccatc acatatacgc
    12781 caccaagtag cggagagctt tttagtcgta aaatggaaaa atatcgggaa actgagaaaa
    12841 agaatcatga ataacaggtg agaatcaaaa gacgaggaag agcagatagt ggctcttcct
    12901 cgtcttttgt actccagtac tatttgttca gaattcaaaa aaaagagaaa tttttttgtt
    12961 tttttacctg taatgtacat aagaagagtt aaaacctttt gttattatta gcattctaac
    13021 aaaaaaatgt cttgatttat taaagcgctt tctatagaat gaaaaacgaa taaaaactaa
    13081 aaaaggcacc cgacaattta gtagcagcta aattgtcggg gcttgaaaag tcagcgggaa
    13141 ctgaccaatc aagttgccat agtcaatgct tgtgcatcga cgtgtatatt gtacctaatt
    13201 tcatacagaa ttactagtta tttttctgtt ttttagggaa gagcgcacac gaagaagcat
    13261 gcttatttag ctatgtcaag gatattgggc agttgtctga tatccttttt tctttgcgta
    13321 attatcctcc catcattgaa gcaagaagtg tttttagcga tgaaagaaga atcagcacta
    13381 tctaattttt cttgaaagta ttgagttgtt catcctaggc agaacgataa ggcaagtctt
    13441 ggtttttatt ccccttgaaa aagatcgttt aataaatatg ttctgtataa aaatacagta
    13501 tattgaaaaa taacaatgga agtagaggtg cagagaatga agaaaagaat gagaatcatc
    13561 ttagctttaa gtgttatgtt attcttcttg acggcttgtg ggcaagaaga tgacaaaaaa
    13621 atcacgctga acatggcttg gtggggttcg caagtccgac acgatgcaac agtaaaagta
    13681 atcgagcttt atgaagaaaa aaatccccat gtgacgatcg attatgaatt ttatgattat
    13741 gaaggatatt acacaaaatt aggtacacta gcagcatcag atgatatctg ggacattttc
    13801 caactgaaca atcaatttcc tcaatacatc aatcagattg aaccattaga tgaatatgtg
    13861 gagcaaggga taattgatat ttctaatgca gatgaatcct atttagcaac gacgacttac
    13921 gacgggcaat tagttggact gtctaacggg gtgaattcgt ttgcgattgc ttacaataaa
    13981 aatatatttg aacaattaaa tatccctgaa ccagcttcaa attggacatg ggatgaattt
    14041 gaacaaatca gctatcaaat cactgaagaa ttaggaatct atgctatttc aagattcgaa
    14101 gattttatag ctggctgtat tattcaaata ccacaagttg agaaaggatt gaatttttat
    14161 aatcgggaag atctatctaa cagcttggga tttgaaaatc cagactatct aacagatttt
    14221 ttccagctgc gtaaaaatct ggtaaacaaa ggggcccatc cagaaccagg tggtggagct
    14281 gcaagtacga cggatctgtc ttttcaggcg gttagggata gagaagcggc aatggtgttt
    14341 ctatcaagta accagttgac agaaatattg tcaggggctc ctgaagatat ggagattcag
    14401 ttgattaatc cacctcgcag aaaagcagat ggagaatctg gtatcccttt acgttcttcg
    14461 caaatgctga gtatggcaaa aagctcagcc cataaagaag aagctgctaa gtttattgat
    14521 ttctttcaaa acagtgaaga agccaatgaa attttaaaag gagaacgtgg tgtaccaatc
    14581 atgtcgaata ttcgagaaca gctcgctcgt gcagatgatg caactttgca agcgacattt
    14641 gattatttag aaatgatttc cgatatggac aacggcgaag tgaatgtctt tgatagtgct
    14701 ttgaatccgg aaattcaaga ccaatacaat cttttagttg aaaaagtcat ttatgaagaa
    14761 gtaacagcag aagaagcagc agagagtctt tatgagtttg cgtcaaacat tctaggatag
    14821 cagcaggagg aaggcatgaa aaaagaaaaa ctaaaacggt tcttggatca agacaatgtg
    14881 gtcggttacg tatttgcttc tccattcatc ctaggatttc tattgataac agtgataccg
    14941 atgatcttgt cactttatta ttcgttcacc gattatcgtt tgggaaatcc agtctcatgg
    15001 atcggcttgg ataattatat aaagatgctg agtgatgccc gttttttgaa ctccatccga
    15061 gtcacttttc tctatgtatt tgcatctgta cctgtgaaac tgatctttgg tctattgatt
    15121 gcttatcttt taacgaggaa tatcaaagga acgactttct ttcgttcttt gtattatgtg
    15181 cctagtttga tcggtggcag tatttctatc gctctagttt ggcgggagtt gttttctaaa
    15241 aacgggttag tgaatatttt cctggaacga ttaggcgcac ctgcgctttc ctggtttagc
    15301 gatcaaaaac tggtcatgat ccctcttgtt ttgatgtctg cttggcaatt cggttcggca
    15361 atgatcatct ttgctgcagg tatcaaagag atcccaggct cgtattatga agcggcagag
    15421 atcgatggag cgaacaaatg gcagacgttt tacaagatca cgttacctct tctttcgccg
    15481 atcattttgt ataacttggt tatgcagaca atcgcggcgt ttatgacttt tacgcaggca
    15541 tttatcatta cccaaggtgg tccgaatgat gccacgaact tttatgctct ttatgtgtat
    15601 aaccaagctt tttcttttaa ttctatgggg tatgctagtg ctatttcgtg gatcatgctg
    15661 ttgattatgg gagctgtcac ctttctgatt ttcaaatcat caaaaaaatg ggtcgttgtg
    15721 tgaatgagag gaggaagaaa tgaataatac agtaacgaaa tggtttcaat acggttttat
    15781 tttgctatta ggtctagtca tgatttatcc ggtcttatgg atgatcgcag gttcttttaa
    15841 aacggatcaa gagattatga gtggttcgct gaatttgatt ccagagactt ttcaatggga
    15901 aaattacgca aaaggatggg cggggtttgc tggaatttca ttttttactt tctttaggaa
    15961 ttcgtttgtc atctctgcag tttcgacgat agggacagtt ctgagctcga cttgtattgc
    16021 ttacgcatta tcgagaatcg atttcaaagg aaaaagattc tggttcgtcg tcatgttagt
    16081 aacgatgatg attcctgcac aagtgatttt gattccgcag tttattattt acaatcggct
    16141 gaatttggtt gggacgtatg tgccgctgat tttaccacat ttttttggac aagcattttt
    16201 catttaccag atcatgcaat tcatggtcag catccccaaa gagttagatg aatcagcgat
    16261 tattgacgga tgcagcaagt atagtgtctt taccaaaatt atttttcctt tgctgaaacc
    16321 ttcgatcatc acgacgatca tcatccagtt ttattggaaa tgggacgact tcatgggacc
    16381 attgatttat ttaaacaagc cgagatctta cacggtatcg attgccatca aattatttgc
    16441 agacgctgga tctagtacga cttacgcgag tatgtttgcg atgtctacgt tgtcgctgct
    16501 accggttttt ctgattttct tattcttcaa taaatatctt gtccagggga tcagtacaag
    16561 cggtctgaag ggctgaatat gaaaaagcca atagaagaga tgtttcttta caagctcaac
    16621 tactatgtct gtcggatttt tttaaccagt cttatcagta atgctctttt cgctttagtt
    16681 ctaggatgtt tgttcacttt aggattagtg ctgtctgctc gtgttgtcta tctgatttat
    16741 tttttgatga tgtatccttc ttattatcta tctgtccatt acttggcaga aaaaaaggta
    16801 tgttggagga aaggaaaaat caaacggatg tgtttcatca ttacgttgtt tcagctgttt
    16861 gtcattattt ttgcagttga ttggtattta ttagtgggaa agattggagc ttggtatctg
    16921 attccttttc tattgtgtct gttgcttttt gtatcccata gtttttttag tacgctaatt
    16981 gatatctgca aagaagataa agcagatagt cttgtttatt tgatcaaaag gaatatctgg
    17041 ctgttgtcaa aaaacgtcaa ggggtttctg tggtatttat gccttttgta tggactgttc
    17101 tggtgtatac agcatgattt tttactcact gcattactgt atccagggct gccatttgtc
    17161 acagtatttt gcttaaaaga aaaggagaat gactttaaat gaagaaaaaa tatgtacaag
    17221 tcggacttgg tgggagagcg cgtttttttt atgaggcaat tgcctctacg tatcatcaaa
    17281 cttgtgaact agtcggtttt tgtgatgtat ctcaaacaag gatggattat gcaaatcgga
    17341 tacttgtgaa caagtatgga cagaaaaaag taaagacatg tatgccatca caatttgagc
    17401 aactactgga tgacgtacgg ccggctgaag tcattattac gtcagtagat cgaacgcatc
    17461 atgattatat cattcgtgca atgaaaaaag gatatgatgt tatttgcgaa aaacctctga
    17521 ctatcgatga ggaaaaagct caagaaataa taacggtcca aaaacagacg ggaagaagcc
    17581 tgcgagtcac ttttaattat cgttatgcac cgtatcacac gaagataaag gaactgatag
    17641 aaagtgatgt gattggtgat gtgttttcta tccacttcga atggctgtta aatacgcaac
    17701 acggggcaga ttatttcaga agatggcatc gaaataagca caatagcggc gggcttcttg
    17761 ttcataaatc tacccatcat tttgatctag tgaatttttg gttgaacagc cgtccccaaa
    17821 aggtatttgc attcggtgga ttaaactttt atgggatgca taatgcggag aaacgcggag
    17881 tagaaatatt ttacagtcga tgctatggaa gcgaactagc taaagctgat ccatttgcta
    17941 tagacctgtc agccaatgag gaattgcgag aaatgtattt agaagcagaa aaagacagtg
    18001 gatatattcg tgaccgcagt gtattttccg atgatatctc tattgaagat acactaggcg
    18061 taacggtcaa atatgaaact ggtgcgattt tgacgtattc gctaaacgcg tatatgccgt
    18121 gggaaggatt caatgtttct ttcaatggca gcaaaggacg gatcgaaatg aaagtgatcg
    18181 aaaagtctta catcaatgca ggtggagata aaaaggatga aggagcagcc gttcttcagt
    18241 ccatccatgt ctatccgatg ttcggtgagc catatgaggt aatcgtcgaa caagaagcgg
    18301 gcggacatgg cggaggagat ccgcgtttat tagatgatct atttggtgaa gaaaaagaag
    18361 aagatccgtg gaatcgggca gcaactcacg tagatggtat tttgtcgatt ttgacaggaa
    18421 tcgcagcaaa tcattctatt gcttcaggaa aagcagtagt gattgacgag cttgtcagct
    18481 taaaagaata agaatagatt tacggaaatt acttcccgta gtttataaaa tcttgcctta
    18541 ttgccgatag gctgtctttt gaacactatt tattcggaaa aaggggctat gacatgcttt
    18601 ttgtcatgcc ttctttttat gtgggctgct tttttagata ggagaaaata aaaataacta
    18661 tttaaacccg aacaaaaagg aataatgcat taaaaatcat tagattttac aaaaaaataa
    18721 gtaaaccacg ttgtcagttt cagaaagtag tgttagactg ttttcaatat ttgaaacaac
    18781 attttttatt caaatatggc aaactgcctt cacgtgcaaa acagttgcaa gaaaatgaat
    18841 ttacactatt attagtttta catacctact tatatatcgt cataatatgg atgacagttt
    18901 ctagccagta ccgtaaatgc tggactataa gtaaaagatt ggctatttca cccttctttt
    18961 cttatgataa agtggagggt gtttttcttt aacagaaaca tagtaggttt ttgcacaaaa
    19021 gcggttattt cggtattaat aaatgataaa ggagcaaata aagtgaaaga attagttgaa
    19081 cgtattcaaa aagacggacg tatattaggc gagggcgtat taaaagtcga cagcttcgtc
    19141 actcaccaag tagaccctgt attgatggaa caaatgggaa aacggtttgc agaagtattt
    19201 aaagaacaag gaatcacgaa agttgtgacc attgaagctt ctgggatcgc accagcttta
    19261 tttgctgcaa gagaattaga tgttccaatg atcttcgcta gaaaagcaaa aagtttgacg
    19321 atggatgaag aattattgac agcttctgtt tattctttta cgaaacaagt gacaagtacg
    19381 atttctattt ctcgtaaatt tttatcttca gaagacaaag tattgattat cgacgatttc
    19441 ttggcaaacg gacaagcggc aaaaggattg atcgaattat gccagcaagc tggagcgcaa
    19501 gtagaaggaa tcgggatcgt catcgaaaag tctttccaag atggacgtca gttacttgaa
    19561 gaaatgggat tgagagtcgt ttctcttgcg cgtattgctt ccttgacgaa tggacaagtt
    19621 gagtttcttg aggaggacgc ataatcgtgg aaaaagaaac acaaaatgga aaagctgcgg
    19681 ttttagggct gcagcatctg ctagcgatgt atgcaggagc agtagcggtg cctcttttga
    19741 tcggtacagg actaggcttt aatcaagaac agatgactta tttgatttca attgatatct
    19801 ttatgtgtgg attagcgaca ttattgcaat tgacagttaa ccgatttttc ggaattggct
    19861 tgcctgtcgt tttaggatgt gcgatccaag cagtagctcc actgatcttg atcggaacca
    19921 atgaaggagt aggagctatc tacggatcta ttatcgcttc tgggatcttt gttgtgttag
    19981 tttctggtgt cttttctaaa attaaaaaac tattccctcc tatagtaaca ggaacggtta
    20041 tcaccgttat tggtttgact ttgattcctg tagcaatcga gaaaatgggt ggaggtaatg
    20101 caacagcagc tgattttggt gacaaaacaa atctgctact tgcatttgtg acgattctcc
    20161 taattgtagg cgtgcaaatg ctggcaaaag ggttcattcg ttcaatcgcg gtattgattg
    20221 gtttagtcgg aggaagcatc ttagctgctt tcatgggaat gattgatctg agcgcgattg
    20281 gagatgcgcc gttgttccat gttccacaac ccttttattt tggtaaacca actttcgacg
    20341 tatggtcgat tttactaatg atcctcattt ccatggtcag catggtagaa tcaacaggtg
    20401 tatattttgc acttggtgac attactggga aaaatgttgg agaagaagaa ttgaaaaagg
    20461 gatatcgtgc agaaggactt gccgttatct taggcggtat tttcaataca ttcccttata
    20521 ctggcttttc tcaaaatgtc ggattggttc aactttcggg aatcaaaaca agaaaaccca
    20581 tctatttctc tgcattcttc ttgattgttt taggattatt ccctaaaatc ggtgcgttag
    20641 cacaaatcat ccctgagccg gtacttggtg gaggaatgtt agtcatgttc gggatggtgg
    20701 cagtccaagg aatccgtatg ctgttggaag ttgatttcag caatgataaa aatctattga
    20761 ttgttgctgt ttctgttgga tttgggctgg gtttcaatgt tacgccgaca ttattcagtc
    20821 aaatgccgca aacattgcaa atgttcactg gaaatggcat cgtaatgagt agcattacgg
    20881 ctatcatatt gaatttgatc tttaatggat tgaaaaaaga tgaagaaata ttggaagaag
    20941 ccttcaagaa gtaatagagc aaaagaaagg ttgtgaaatc agcgttttga cctgattgtt
    21001 agagaaacaa acggaaaaat agttcctcgt attttgccgt ttgtttgagc taataccgag
    21061 ggtcagctga ttgaacaccg ttgaaggaag aggtcgtgag cctgccgttc atctccgagt
    21121 cacaaggaac aatttcaaaa aaaagcttct catgtttgtt gaatattgtt ccttgtgacc
    21181 gaggagttgg caggagaaca ccgtttatcc ataaaaagga atgtgaccta acttttgtca
    21241 cattcctttt ttttatcttt aataaacacg aacaataaaa tgtttttctt tgttaacatt
    21301 cgaaatttct ttgacatcaa aaaaagtgat tgttaaaata tgaatgtaga aaaactggga
    21361 ggaaaaacaa tgacagcaaa aatctctgtg attatgggga gtacttcaga ttgggaaaca
    21421 atgaaagaag catgtcaaat attggatgaa ttcggtgtgt cttatgaaaa aaaggtcgtt
    21481 tctgctcatc gaaccccaga tttgatgttt gaatatgcac aaaacgctcg aaaaaaagga
    21541 atcaaaatca ttattgcagg agcaggggga gccgcgcatc tgccgggtat ggtcgcatca
    21601 aaaacaactt tgccggttat cggtgtaccg gtccaatcac gaacattaaa tggtttggat
    21661 tcgcttcttt cgattgtcca aatgcctgga ggagtacctg tagctactac agcaatcgga
    21721 aaagcgggag caattaatgc tggactactt gcgatacaga tgctttccat gtatgattta
    21781 gaattggagg ctaaattggc tgaaagacgt tcaatgttgg ctaaaactgt tatagaaagt
    21841 agtgatcaac ttggttaaac ctttgttgcc tggttcaacg atcgggatcg tcggtggcgg
    21901 tcaattaggt cgtatgatgg cgattagtgc aaaagaaatg ggctttaaag taggtgtgct
    21961 ggatcctgtg agcgattgtc cagccgcaca agttgcagat tggcacattg aaagtgtcta
    22021 tgatgacacc tttgcgctga aagagcttgc cagaagaaca gatgtcatta cctatgaatt
    22081 cgaaaacgtc agtgtagagg cgttaaatgc gattttaccc atgtcattta ttcctcaagg
    22141 gacagacttg cttgcgatta cgcaagaccg cctgttagaa aaatcttttt tggaaacgaa
    22201 taacatcgtg attgctcctt atgcaacgat cgtcagtccg acggatatcc aagatgcgat
    22261 tgacgggatc ggctatcctt gtgttttgaa aaccactcga ggcggctatg atggcaaggg
    22321 gcagtatgtt ttgaaaagtc gggcggatct agctcctgcc atggaccttt taagagaagg
    22381 aacctgtgtt ttagaagcat ggatcccgtt tgaaaaagag atttcgatca tggtggcagg
    22441 aaatggtcaa ggagatttca cgacttttcc ggtcgtggaa aacatccacc acaacaatat
    22501 tcttcatgaa acgattgctc cagccgcgat tgatcaggat gtgatcgagg aggctgagcg
    22561 tattgcccgt gtgattgcag aagcagtgag cctttcaggt gttttagggg tagaaatgtt
    22621 tttaacaaaa acaggcgggc tatatgtgaa tgaattagct cctcgtcctc acaattctgg
    22681 acattattct attgaagctt gttctatgag tcagttcgat gcccatattc gaggtatttg
    22741 tggttggcca ttaggagaaa aagtacgttt gataagcaat gcagtaatgg tcaatgtttt
    22801 gggagaacaa ctgctggaaa gctatcgctt gattgagaat aagccagact ggcaattcca
    22861 tttctatgga aaaaaacaag caaaaaaagg tcgcaagatg ggacatatca ctatcttgac
    22921 agaagacatc cagcaaacgc tggaagaaat caaacaaacg aatatttggg attagaggag
    22981 aaaaaacatg ttagaacgtt atacacgccc tgaaatgggg aatatctgga cggaagagaa
    23041 caaataccgt gcgtggctcg aagtcgagat tttagcagat gaagcttggg cagaacttgg
    23101 tgaaatcccg caagaagatg tgaaaaaaat tcgtgagcat gcatcatttg atatcgatcg
    23161 gatcttggaa atcgaaaaat taacacgcca tgatgtcgtt gcgtttacac gagcggtttc
    23221 tgaaacattg ggagaagaaa gaaaatgggt ccattatggt ctaacaagta cggatgtcgt
    23281 agatactgct tatggctatc tcatcaagca agcaaatgat attttacggg aagacttgaa
    23341 acgttttacg gaaatcattg gcgaaaaagc aaaagaacac aaatatacag tgatgatggg
    23401 tcggacacac ggtgtccatg ctgaaccaac tacttttgga ttgaaacttg ccacatggta
    23461 ttcagaaatg aaacggaaca tcgaacgatt tgaacatgcg gcaaaaggag tagaagcagg
    23521 aaaaatctca ggagcagtgg gaacgttcgc aaatatcccg ccatttattg aagagtatgt
    23581 atgtaaacaa ttggggatcc gtccacaaga aatctctact caagttctcc ctcgggatct
    23641 acatgctgaa tatttctcag cgatggcgct tattgctaca agtattgaac gttttgcaac
    23701 agagatacgc ggattgcaaa aatccgaaac acgtgaagta gaagaatttt ttgcgaaagg
    23761 tcaaaaaggt tcttctgcta tgccgcacaa aaggaatccg atcggttcag aaaatatggc
    23821 tggtctagcc agagtggtgc gcggacacat ggtcactgcc tatgaaaatg tcacattatg
    23881 gcatgaaaga gatatttctc attcttcagc agaacgaatc atcttgcctg ataccacgat
    23941 tttattgaat tacatgttaa atcgttttgg caatatcgtt aaaaatctaa cggtctttcc
    24001 tgaaaacatg aagcggaata tgtctgcaac atttggtttg atttatagcc agcgcgttat
    24061 gctgaaattg atcgataagg gaatgacaag agaagaagct tatgatcttg ttcagccaaa
    24121 aactgcgtac gcttgggaca accaagtgca gttccgcccg ctgttagaag aagatccaac
    24181 catcacatcg gtattatcta aagaagaaat agacgatgcc tttgattatc atcatcattt
    24241 gaaaaacgtt gacgtgatat tcaaacgagt aggtttggaa taaagaagag tgagttgcaa
    24301 aattcaatta aaatagaata gcaaaaataa ccaaaaaaga agcagatccg ttagcggttc
    24361 tgcttctttt agtttattat attttcttat caataatagg aacctgattt taaaagcggc
    24421 tacctcttta ccgtgcctct ttacatacaa aaaatagagg aattaagcgt aaatggcttg
    24481 gctattataa ctaaaatgct ggttatggag atgttgcagc aagttttcga gaaaagcgta
    24541 ttccatctct tgtccattga ataagatctg gatacgataa tgatttttga aacgcttctg
    24601 ttcatgaatc tgactgattg aaagttcgcc tttgtgttct gtctgataaa gaaaagtcct
    24661 tttacggcat tcagcacagc gcttggttgc tcggcggatt tttcggaatg tataaaaact
    24721 agtatcttta ttgcataaat aaacgtcttt gatcgacagc aaatcattat atagacagga
    24781 acgttcttca gctgttaatt ctgataaaaa aagctgacct tgttttattg catcttccat
    24841 cggcaagtgc ctcctcgaac ccattatcca aatttttagt taattctaat ataccatcat
    24901 taagtataaa tgtaaacgct tgttgatggt ataagatttt cgtttctgat aaaacacgaa
    24961 cattaaaaga atctaatgcc ttaaatgtac atatttttgt tgatttataa aaagatgatt
    25021 gctaaaattt aaagtgaaaa aacgaacaaa aaacgatgta tttgctttta atgtaaataa
    25081 aaaggagaga cgtacatgga gaagaaagaa cttttgtatg aaggaaaagc caaaaaaata
    25141 tatcttactt ctgatgagaa cttgatttgg atagaatatt tagatcaagc aactgcgttg
    25201 aatggcgcga ggaaagacca gataagtgga aaaggagaat tgaataatca aatcacaagt
    25261 gcaattttcc gttatctgac tcaaagagga ataaaaaatc attttattga acaattgtct
    25321 aaaaccgaac aattagcaaa aaaagtacaa attatccctc tggaagtggt tatccgaaat
    25381 attgcagcag gaagtttctc aaaacgtcta ggtgttcccg aaggtaaaga attcatcaaa
    25441 ccaatcattg aattctacta taaggatgac cagttggatg atccatttat caatgaagag
    25501 catatctatt tcttacaact tgcaaagccg gaagaaatca gtgagctgaa agcccaagcg
    25561 ttggctatta atgaaatttt gaccgaatta tttgctaagg tcggtttggt tttagtagat
    25621 ttcaagctag aatttggccg gtcgaatgga caaattttgc ttgcggatga agtaacaccg
    25681 gatacttgcc gattatggga tgaacgtacg aatgaacata tggacaaaga cgtttaccgc
    25741 agagatctag gtgatttgat cccagtctat caagaagtat ataaccgtct atcggaacta
    25801 actgaagaaa aggagcgagt atgatgtatt tcgtaaaagt atatgtaacc tataaagatt
    25861 cagtgttaga tcctcaaggg gaggcagtaa aaaaagcagt ccatcgtatg ggatatgaaa
    25921 caatcgaaga tatccgaata ggaaaatact ttgagataca acttgctgat gcagaagaac
    25981 caatcgatac agtgattgaa acgatttgtg acaagctgct tgcaaatgta aatatggaaa
    26041 cataccgata tgaaatcaaa aaaatggagg aagtataaca tgaaatttgc ggtcatcgtt
    26101 tttccaggtt caaattgtga cgcagatatg ttatgggcag ttcgcgagat catgggcgct
    26161 gatgccgaat ttgtacggca tgatgagtcc tctttagaag gatttgatgg ggttttactg
    26221 ccgggaggat tctcctatgg tgactatttg agatgcggtg cgattgctcg gttttctaca
    26281 atcatggaag aagtgattcg ttttgctgaa gaagggaaac cagtattcgg tacatgcaat
    26341 ggattccaga tattgacaga agcaggactt ttgccaggtg tactgagacg aaatgagtct
    26401 ttgcatttta tttgcaaaac tgtaccattg aaagtcaacc ccaagacggc ctttacttca
    26461 gagtatgaag ccgacgaaat aatctatctt ccagttgctc atggggaagg aaattatttc
    26521 tgtgacgaag aaacacttca agaactaaaa aaaaataatc agattgcctt tacttataat
    26581 gacaacatca acggcagcgt ggaaaacatt gcagggatta cgaacaaagc agggaatgtg
    26641 ttaggaatga tgcctcatcc agaacgtgca gtagaagact tgctaggatc agaagatggg
    26701 aaacggttct ttgcttccat tttaaaaaac ttcggaaagg tgaccgtata gatgaaaaca
    26761 ttgaaagaac cgacagctga agagatcaag aaaaaaagaa tctatgccga atggggattg
    26821 actgatgaag aatatcgaat gatcgaagaa gatatcttag gaagaatgcc taattatacg
    26881 gaaactggac tgttttctgt tatgtggagc gaacattgtt cctataaaaa ttctaaaccg
    26941 gttctacgca aatttcctac tagtggacct catgtgttgc aaggccctgg agaaggcgca
    27001 ggcatcgtcg acatcggtga tggactagca gttgtcttca aagcagaaag tcacaatcat
    27061 ccttcagccg tggagcctta tgaaggagct gcgacaggag ttggcggaat catcagagat
    27121 attttcagca tgggggctcg tccaattgcg ttgctagatt cattgaggtt cggcgaatta
    27181 gataatccaa gaacgaaata tttactagaa gaagtagttg caggtatcgg cggatacggc
    27241 aattgtatcg gcataccgac tgtaggcggc gaaattgcat ttgatccttg ttatgaagga
    27301 aacccattag tcaatgcaat gtgcgtaggt ttgattgaac acaaacatat ccagaaaggg
    27361 caagccgctg gcgtcggtaa ttcgatcatg tatgtaggtg caaaaacagg tcgcgacggt
    27421 atccacggag caacttttgc ctcagaagaa tttaatcaag aagaagaaca gcaacgttct
    27481 gctgtacaag taggcgatcc ttttatggaa aagctgctgc ttgaagcgtg tttggaatta
    27541 atcttgcatc attcggattc tctaataggt atacaggata tgggagcagc aggacttgtt
    27601 tcatctagtg cggaaatggc ttcaaaagct ggctcaggac ttatattgac attagatgaa
    27661 gtcccacaaa gagaaactgg gatgacacct tatgaaatga tgttatcgga atcacaagaa
    27721 cggatgttga tttgtgttaa atcaggagag gaagaaaaaa tccaagaact atttcagaag
    27781 tatgatctag atgcagtcac tatcggtaaa gtaacagacg acgggcaata tcggctatat
    27841 catcaaggag aagaagtagc aaatctgcca gtcgatgcat tagctgaaga tgcaccagtt
    27901 tatcataaag aaatgaaaga accagctcga atcgtagaat ttcagcagtt agctccttat
    27961 cagccagtta tcgaagaacc tggacaagta ttgcttgatc tgctgcagca accgacgatt
    28021 gcttcaaaac gttcaatcta cgaaacttat gattcacaag tacaaaccaa tacagttgtc
    28081 cgtccaggaa gcgatgctgc tgtgatgagg gttagaggga cgaataaagc gttagctatg
    28141 accacagact gcaacgcgcg ctatctgtat ttggatccaa aaatcggagg acaaatagca
    28201 gtggcagaag ctgctagaaa tattattgct agcggtggta agcccttggc gatcaccgac
    28261 tgcctgaact atggttctcc tgataaacca gaagtttttt gggaattatc gacttcagct
    28321 gacggaattg ctgctgcgtg tgaaacgtta ggaactcctg ttatttcagg aaatgtctct
    28381 ttatataatg aaacggatgg tcaagcaatc tatccaacac caatgattgg tatggtcgga
    28441 ttgattgagg atcataaaca tatcactact caggaattca aaaaatcagg ggatctaatc
    28501 tatatattag gtaagacgtt tgcagatttt gatggcagtg aattacaaaa gatgcagctt
    28561 ggaagaatcg aaggtgtgat ccgaaacttt gatctatcca ttgaaaagag aaaccaagag
    28621 ttagtattga cagccatcca aaacggattg attgaaagtg ctcatgactg ttcagaaggt
    28681 ggcttggcaa ttgcactggc agaatcagct ttcaaacatc agctagggat atctgtacag
    28741 tttgaattgt cctctgctca attatttgca gaaactcaat caagatttgt gttgacagtc
    28801 gcaccggaaa ataagacacg attcgaagaa atgatgggag atgctgctgt tttagcaggt
    28861 aaggtgacgg atgaagcgat aatcgaaatt tcagcaactg acggtcagat caaaatagaa
    28921 actgcagtag caagaaagtg ctgggaggat gcaatcgtat gtcttatgaa gtgaagagtt
    28981 taaatgaaga atgcgggatc ttcggtgttt ggggtcatcc agacgccgct agtgtggcat
    29041 acttcgggct ccatagtctt cagcatcgag gacaagaagg tgcaggtatc gtgtccaatg
    29101 atcgaggaaa gcttaagggg catcgagatt tggggctgct ttctgaggta ttcaaagatc
    29161 aaagaaaatt gcagcagtta acaggagaag ctgcaatcgg acatgtacgt tatgcaacag
    29221 caggcaatgg aagtgtggac aatattcagc cttttctatt caaatttcat gatcagtcga
    29281 tcggcttagc gcataatggt aacttgatca atgctcgttc ccttagaaaa gagttagagg
    29341 aaaatggggc gatcttccat tctaactccg atacggagat attgatgcat ttgatccgac
    29401 gaagtgaaga aacagatttt ttaaataaat taaaaagtgc tttgaatgaa gtcaaaggtg
    29461 gctttgccta tttattaatg acagaaacgg caatgattgc cgctttggac ccaaatggtt
    29521 ttcgtccatt agcaatcgga caaatgaaaa acggagcata cgtgattgcc tcggaaactt
    29581 gtgcattaga agttattggg gcacgtttta taagagatgt ccagcctggt gagatagtga
    29641 tcatcaatga cgaaggaatc cagattgatt gctttacgaa ggacacgcag ctttctattt
    29701 gttcgatgga atatatctat tttgccagac cagattcaaa catcgcaggg atcaatgtgc
    29761 atactgcacg gaaaaatatg ggacggaatt tagcgaaaga atcgccagta aaagcagata
    29821 tggtcgttgg cgtacctaat tcttctttgt cagccgcaag cggttatgcg gaagctagtg
    29881 gaatccctta tgaaatcgga ttagtaaaaa atcaatacat tgcccgaaca tttatccagc
    29941 caacgcctga gctaagagag caaggtgttc gaatgaaatt atctgctgta cgaggagtag
    30001 tggaaggtaa aaaggtgatt atggtcgatg attcaattgt tcggggtacg acaagcagac
    30061 ggatcgtcca tcttttgaaa gaagcagggg caaaagaagt ccatgtgagg attgcttctc
    30121 ctcctttgaa atatccatgt ttttacggga ttgatattca aacgcgtaaa gaactcattg
    30181 cggcaaatca cacgattgat gaaatcaaag aatgtatcgg tgcagattct ttaggttttt
    30241 taagtgaaga gggtctgatc gaatcgatcg gtctggaaaa agatgctccg tattcaggat
    30301 tgtgtatggc ttatttcaac ggagattatc cgacgccgtt atatgactat gaagaaaaat
    30361 atcaggaatc actaaaagaa aaagtatcgt tcttttaaaa gcagaccaag aagggagtat
    30421 atgaattcaa tggcaaatgc atacgcaaaa gctggcgtag atgtcgaagc tggatatgaa
    30481 gtagtagaac ggatcaagaa gcatgtcaaa aaaacggaac gtttaggtat gatgggcgct
    30541 ataggaggat tcggcggttg ttttgattta tcggtctttc aattaaaaga gccagtcctt
    30601 gtctcgggga ccgatggcgt ggggacgaaa ttagcagtgg caatcgaagc agacaaacac
    30661 gatacaatcg ggatcgactg cgtagcgatg tgtgtcaatg atatcttagc acaaggcgca
    30721 gagccgttat atttcttaga ctacttagca acggggaaaa atgatccgtt acgtttggaa
    30781 aatgtagtaa aaggtgtagc agatggatgt gttcaaggaa atatggcgct gattggcgga
    30841 gaaacagctg aaatgcctgg tatgtactca ggtaatgatt atgatctagc cggatttgct
    30901 gtagggatag ctgaaaaatc tcaattattg acaggagaaa cgatcaaccc aggagatgtg
    30961 ttgcttggaa tagcatctag cgggattcat tcaaatgggt actctcttgt tcgcaaaatt
    31021 ttctttgagc agcagcagtt ttctttatca tcaaaactgc cagaattgaa aaaaacactt
    31081 ggcgaagaat tgcttgagcc aacgaaaatc tatgtttctt ccttgcttcc gctgatcaaa
    31141 gaaaagaaaa taaaaggagc tgcacatatc actggtggag ggtttgtaga aaatatcccg
    31201 cgaatgttac cagaatctat ggctgcacaa atcaaacttg gctcatggcc gattcttcca
    31261 atattcggct gtttacaaaa atatggtgaa attcctgaga tggagatgta tgaaatcttc
    31321 aatatgggca tcggtatggt cctggcagtc gaagaatcga aagtaaaaga aatagtacac
    31381 gaaattgagc aagctggcga aaaagtttat gaaatcggga aagtgatcgc caaagaaaaa
    31441 catcgtgtgg tgtttatagg agaagaacga tgagaatcgc tgtttttgct tctggaaacg
    31501 gaagcaactt tcaagcgcta gccgattatc tatcgaaaaa aggattggaa tcttcgattg
    31561 actggttgtt ttgtgatcag cctgaagcat atgttttgaa acgtgcaact gcgttgagtg
    31621 taccggcaga ttgtttttca cctaaagaat ttgattcaaa aaaagagtat gaagaagcaa
    31681 ttttgcataa attgaaagaa aaaaagatcg atttgatcgt attagctgga tatatgcgaa
    31741 tcattggacc ggtattgctg aaaaattatg ataaaagaat catcaatatc catccgtctc
    31801 ttttacctgc cttccctggt ttgcatggca tacgtgatgc ctttgaagct ggagtaaaag
    31861 aaacgggtgt cacaattcat tatatcgacc aaggtgtaga tactggacca atcatccggc
    31921 aggaaaaagt gagaattgaa caggaggaca cgttcgattc tttagaagaa aaaatccata
    31981 gagtagagca tcgaatctat ccagaagtga ttagtgaaat tattgaaaat ggaggctatc
    32041 tgaaatgaca agagcattga tcagtgtatc agataaaact gggattgtag cattcgccca
    32101 aggtttgatt gaacaaggag tagaaatcat ttcaacagga ggaacaaagc atgcgttgga
    32161 taaagctggg atcaaaacgc tttcgatcga agaagtgaca gggtttcctg aaatgatgga
    32221 tggccgtgtc aaaacacttc atcctaaaat ccatggtggt cttttaggaa gacgcgatct
    32281 ccctagccat atggaggcaa tggaaacgga aaatatccaa ccaatcgact ttgtttgtgt
    32341 caatttatat cctttcaaag aaacgattat gaaaccagat gtcgaaatag cagaagctat
    32401 tgaaaacatt gatatcggtg gtcctagtat gctccgtagt gcagcaaaaa accatgaatt
    32461 tgtcacctct gttgttgatc catctgacta tgaagaagta ctttcagaat tacgtcagta
    32521 tgggcatact tcactttctt tcagacaaaa actagcggca aaagtctttc gtcatactgc
    32581 ggcatatgat gcactgatcg ctcaatactt aacggattgg gtggaagaaa aagaaccgga
    32641 aaaattgaca cttacgtatg aacgaaaaca aacgcttcgt tacggtgaaa atagccatca
    32701 agccgctgcc ttttatcagt ccgctcttct ggaaaaatat tcgatcgcag cagcaaaaca
    32761 actccatggg aaagaacttt cttataataa tatccgagat gcagatgctg cgctacggat
    32821 catgaaggaa tatgacgagc caacggtagt cgccttgaaa cacatgaatc cttgtggtat
    32881 tgggacagca tctacgattt tagaagcgtg ggaagcagcg tatgaagcag atcctgtttc
    32941 aatctttggt gggatcatcg ttttgaatcg agaagtagat ctgccaacag cagaacagat
    33001 gcataagctg tttctagaaa tcatcattgc cccttcttat acacaagaag ctttggaatt
    33061 actgcaaaaa aagaaaaaca tccgattact gacagtagac tttgcgcagg aagaaaatca
    33121 aaatcgagaa gaaacggtct cagtcttagg tggacttctt gtacaagatc aagatctggc
    33181 aatagaaaat gaagaagagt ggaaagtcgt gacaaaacgt aagccgactc cagcagaagc
    33241 aaaagcatta gcgtttgctt ggagagctgt caaacatgtg aaatctaatg cgatcgtact
    33301 ggcaaacgaa cgtcagacag taggaatcgg agccgggcag atgaatcgag tcggttcagt
    33361 aaaaattgcg attgaacaag cgcaagagaa aatggaaggt gcagttttag ctagcgatgc
    33421 ttattttcca atggatgatt cggttgaata tgcagccaaa catggtataa aagcaatcat
    33481 tcagccagga ggaagcatca aagaccaaga ttctattgat atggcgaata aatacgatct
    33541 agcaatggtc tttactgatg ttcggcattt tagacattag agaggagtcg gataaatgaa
    33601 cattttggtg atcggtgcag gtggtcgtga acatgcaata agtaaaaaac ttttagccag
    33661 tccgaaggtg gatacggtgt attgtgcgcc aggaaatccg gggatgacaa aagatggtat
    33721 caattgtgtc gctatcagtg agcgagatca ttcagcgtta atccattttg ttaagaaaga
    33781 ggctattgat tggagtatcg ttggtcctga agttcctttg ttaaatggga tcgtcgatga
    33841 ttttatggcg gaaggattga aaatcttcgg tccgacaaaa gcagcagctc tgatcgaagg
    33901 ttcgaaaagt tttgctaaac aattaatgga tacttatcaa atcccgacag ctaaatccag
    33961 aagttttgaa tgttacgaaa aagcagcagc gtatatcaaa gaaaatggtg cgccaatcgt
    34021 agtgaaagcg gacggcttag cagctgggaa aggtgtagtc gtggctacca cagtgaagga
    34081 agcgctggat gcggcagaac aaatgttgaa ccagcatcga tttggggata gtagcaaaaa
    34141 aatcgtcgta gaagagtttt tggcaggaga agaattctcc cttttggcgt ttgtccgtaa
    34201 taccaaggtg tatccgatgg tcatttcgca agatcataag cgggcatttg ataacgataa
    34261 agggccaaat acaggcggaa tgggtgctta ttcaccagta ccgcaaattt caaaaaagat
    34321 gattgaaaaa gctgtggaag aagttttaaa accagcggct ttgggaatga aagaactagg
    34381 acgctctttc acgggtgttt tatatgcggg tttgattgct acgaaggaag gaccaaaagt
    34441 gatcgaattc aatgcgcgtt tcggtgatcc tgaaacgcaa gtcgttcttt ctcggttgaa
    34501 gagtgacttt gcgcaagtaa tcgatgattt attagagaat cgtacagtag agttaaagtg
    34561 gcagcaagaa ggatacaatg taggcgttgt ggttgcagct gccggttacc ccgaagaata
    34621 tgagacagga atggttctac cagattttct ggaagaagag ctttctgttt actatgctgg
    34681 agtttcctca aataacggca aactagttgg agcaggagga cgtctttatt tagtagaagc
    34741 atacggtgag acgatcaaag aagcacagaa aaaaatttac accgcgttaa tggataaaac
    34801 gacagatgga acattttatc gtacagatat tggagaaaaa gctgccaata gcgaactgat
    34861 cgtgaagtaa atcagctgct tccatttata aaaaatacaa caaacataat cggaagcgcc
    34921 gattttagtg aagccttttc ataaagagtg tggtacactt ttgttgtgta tataaataag
    34981 gaggagttca gatgaaaaaa attcaattag gtacttctga tcagaaagca gctgcagtga
    35041 tcttaggctg tatgcgtatc aacaatgcgg aagatccagt aaaagtgatt gagacagctt
    35101 atgaaaacgg cattgatttt tttgaccatg cagatattta tggaaacggt gaatgtgaag
    35161 aaatctttgc agatgctcta gcaaaaacat caatcaaacg agaagatctt tttattcaaa
    35221 caaaatgtgg aatcgttcca ggtgtgatgt ttgatttttc taaaaaacat atcattgaat
    35281 ctgtagaaaa aagtttgacc cgtctgaaaa tggattatgt ggatgcttta cttcttcatc
    35341 gtccagatac attggtagaa ccagaggaag tagctgaagc gtttgatgaa ttggaacggt
    35401 caggaaaagt tcgttatttt ggcgtcagca atcaaaaacc aatgcaaatc gaattgctga
    35461 aaaaatatgt tcgtcaacca ttagtagcta atcagctgca atttggcttg aaacatactg
    35521 gaatgatcga tcaagggatt catgtcaata tgacagatga agcaagtatc gatcatgacg
    35581 gtagtgtact tgattattcc cgattgcatg atatgacgat ccaagcatgg tcaccatacc
    35641 agtatggatt ttttgaaggt gtatttatcg ggaacgaaaa attccctgaa ttaaaccaaa
    35701 cattagaaga actagcagct aaatatcaaa cgacaccaac tggcttggct tctgcttgga
    35761 ttttacgcca tccagccaat atgcaagtga tcgccggtac gatgaacgta gggcgaatca
    35821 aggaaatcgc tcaagcttca gaaattaata tgtctcggga agattggtac gaattgtacc
    35881 gtgctgctgg aaacatttta ccttaaagat tgtcataagc aggtcaaaag ggctataaat
    35941 taaaaagaca gaataacttg aaagctgttt tcagcttcga gatattctgt ctttttttat
    36001 tgcaaatgga aagagtatca ttgtacggat ttcgtttatt agttattttt gtttttaagg
    36061 aaaagctgtg tccgatattc ttttggagaa atcccttgtt ttttcttaaa taaataactg
    36121 aaatagttag ggtcattata accaatttca aaagcaatat ctgaaatacg atagtctgat
    36181 tgctgcaata atttttttgc taattcgatt ctttgttctg tcagataatc gatgaatgtt
    36241 tggttaaacg attgagaaaa aatcgtactg aaatgagagg gacttaatgc aacttgttga
    36301 gcgacccaag ttaaggacat atctggatcg gtaaaatttt ctttgataaa atccaatgca
    36361 tggttcaaaa ttggctgata tttgttcctt gaaggctgtg agggcagagc agttaagaaa
    36421 gaaatgaaca tataaactgt cgaacggtaa agcgagtcgt cggcgctgat tgctaagcgt
    36481 tgactggtgg atgtgcaatg aaccatttct tgcgggattt tcttttcttt caggcagaca
    36541 tgctggagta gcttagtcaa gacaaataaa cgatataatc gtgttcgagt tggtgtgcct
    36601 tgtggtttcg tcaagtgttc gatgtactta gatacttcgt ttttcgataa tgaagacaaa
    36661 tcaagtgaga ggtcaaagta aaagacagaa tcaactggaa gtttcgcttc ttgaatcaaa
    36721 tcctcatagc tgataatttg ttctttaagg gaactggcgg tttcaagtat catctgcgtg
    36781 atctcaaaag atgcaggtaa ttcgctgatt ctttctgcga caggaccaat ggcgacagaa
    36841 aaattatttt tttgtccctt ttctaattca tggaccaacg tttgggctac ctgataactg
    36901 gtctcgatca cattttcttc aagagaatca actattaaaa atttgatgaa tcgagaggaa
    36961 acagcagaga agatcacttg ttgattctcc ctaaataaaa acttcaaata atcactgaga
    37021 tgagcgtaat cttcaaagtc tgtactgact tggtttgctg ctaataatac ggtcattttt
    37081 ttccctgcta aagagcgatt gaaagattca gcaagtttca atgtttcact gatcgtaagt
    37141 gagcctttga acaaaccgtt caagaaatga ttttttttca attcgaaaac aagatcagaa
    37201 gaagtgtgcg tttctttttg ctgcaaatct aaagcagtta ctgctttttc tattgcttgt
    37261 ttcaattcgc tacttttaat cggtttaagc agataatcat ctgcctgtac ctggattgct
    37321 cctttcatat aatcgaaatc gtcgaatcca ctaataaata tgagacgagt ccaaggaagt
    37381 agtttccttg cttcttttga aaaagtcaac ccgtccatga aaggcatttg gatatcagtc
    37441 agaatgatgt cgggctgtaa gtcgataatt gaagacagtg ccatctcacc atctccagct
    37501 tcgccgacat aagtgattgg ataataagaa ccaaggacag ttatttgttc ccgcaagtta
    37561 ttgcgaatga ggtattcgtc ttcgatgata aaaactttat acatgtttgt ttcctccaat
    37621 tgttttggga gatagaggga tcatcagtgt gacagttgtt ccttctcggt atttgctgtc
    37681 aatcgtgata ggtttttgat catttccgta ataaagaagg agacgcttgt ttacgttgta
    37741 aagtccgtag ccgatactga aatctgaatc gatccctttg gctaactctt tacgtatttc
    37801 tgccagtttt tctgcaggga tgccaatccc gttgtctata actttgaatt ggatcatctc
    37861 atcaattttg catgtggtaa tacgaataaa tcctttgtga cggacgtact tcgttccatg
    37921 gtatacagca ttttcaatca atggctgcaa gatcattttt aaaatagaaa caggaaaaag
    37981 tgtttctggt atatcgatct tatagtcaag catatggccg tatcttattt ttaagatgac
    38041 tagataatca ttgatatggc taatttcttt ttcaagagga atccagtctt gtcctttgct
    38101 taaagacgta cgaaaaaagc cggaaagtgc atgggtcata gtcagagctt cttcaaagca
    38161 gtcttgttcg atcaaagaaa taatggcatc caaactgtta taaataaaat ggggagtaat
    38221 ctgagcttgc aatacacgaa cttcactttg tgctaaatgg tattgtttca aagcgttctc
    38281 ccgtaaagac tggtttagat cttccgccat tttattcatg ctagcggtaa gtgtttgtaa
    38341 ttccagtgtt tccggcaggg ttaaacgaaa gccaaggttc cctgaagaaa gttgttctga
    38401 catctcgatc agctgattga tcggttcttg gacctttctc tctaaaaagc gacgattctt
    38461 tttcgcatag aaaaaaatgg tactaagaac tccaagttcc actattgtta atagaatgag
    38521 cgagcggaca agtgtcgtat tttttttgct tgctaagtcg atctccacac gaacaaaatc
    38581 ttgtaagata tctgacagca gttgagtgac agaatctacc tgattgatga tctcttcatt
    38641 ggtatccata aaatcttttg ccaagatatt attgatcagc tcgttttgat aatcttccag
    38701 tgtatcgatc gtacgtagcg atacatctaa cgtactaatt tctttaagtg tatttgtatt
    38761 atttttgatc tctcgaatat cattcctaag ttcttctagg atattttcct cattgaactc
    38821 atctacggtt acttggccag aaatcaagtc ccacatactt tccaaaactt tttgatcaac
    38881 cttcaaagca attgcatctg ctttttggat atttgcgatc gtctgttgat aaatcaaaag
    38941 ctgttgcgaa tataacagta tgctcagtag caaagggata acagcaaata gaagcatggc
    39001 tttgtttgct tgggcaaaaa tctgttttat gcttaaattt tttgtcatga tacttctcca
    39061 gtcaataatt tctggttggg atgttagcta tattctgctg ggaaaagaca gtctcggaaa
    39121 taaaaatatc tttaggtaat gtttttcctg tttgaatatt tccaaaataa cgagtaacgg
    39181 tattggctac ataccagccg acatcaggtt tacactcgac tacacaattg acgattcctg
    39241 agcgtagctt ctcgatcata tcttcttgtc catcaatagt tacaatgatc aaatcttctc
    39301 caggtttgat tgatgtttct tttatcgcag ctaaagcacc gtgagtcatt tcatcactat
    39361 ggctgtataa tacatcgatt tcggataaat catttgtttc taaatatcgt ttcatctggt
    39421 cttttccttt catccgaata aaatcaccga caattcggtg aacaatagtc atcggaggtt
    39481 tatggtcgat ggttccatct ttttttaggg ttggtttacg attgattgat tccaaaaatc
    39541 cttcggagcg tagcttggtc ggtgtggagt tctctgaacc agcaagttct aggattctga
    39601 tttcctgttt ctttgaattt gtgaagtgat tgctgacaaa taaacccgcc cgatttcctt
    39661 ctgctttgaa actggggcca atgtgggtga cgaaaagctc gggatcattc gtttgaatat
    39721 gtctgtcgac aagtatgaca gggatccctg ctcgctttgc ttcttttaat acagagtccc
    39781 acccggattc ttgcaaagga gtaaaaatga tcatatccac tttataggca atgaacgtcc
    39841 gtatatcttg gatttgccgt tcctgattca tgtagccatt tcggtagaga acttggtaat
    39901 cttctttttc aagcgcagtg ataattgatt cagtatgctt tttccgccag ctactctccg
    39961 ttcctgattg cgaaaaacca acgactagtg ggtcaggtgc ttcctttgta ttaggtgtac
    40021 accccatcat ttggtaagcg attacaacaa gggctaaaaa aaataaaaca cgtttcactt
    40081 taaatcactc catctggatt tgttagtact atcttatcag ataaaaggga aaatcaaagt
    40141 caaaaggaat caatcgaaaa aaatccaaga aacttaaaaa aatccaagac aaaaaaagta
    40201 cacctaaaaa atttaaagta aatcccataa aaaaatcaat gttaacgctt tctttattag
    40261 ctatactcgg tgtatcaatt gaaatggggg ataaaaaatg aaaaacaaat ttagaaagat
    40321 tgttgggact atcgctattt tgaccgcaag cattctatta agtgcgtgtg gcaattcagg
    40381 atctgcagat gatagtacgg ggtatgtagg aatcgctatg ccgaccaaat cagcagaaag
    40441 atggatcgct gatgggaaca acatggtctc ggaattagaa aaactgggtt ataaaacaga
    40501 tcttcaatat ggagaagata aagtagaaaa tcaagtagca caaatagaaa atatgattac
    40561 aaaaggggta gatacacttg tgattgcttc tatcgatggt tcggctttaa ctgatgtgtt
    40621 agctaaagca aaagaagcag atatcaaggt gatcgcctat gatcgtttac tgatgaattc
    40681 agaaaatgtc gattattacg cgacttttga taattttgga gtaggtgtat cgcaagcagc
    40741 ttatattgaa gagcacttag gattgaaaga aggaaaagga ccattcacga ttgaattatt
    40801 tggtggctcg cctgacgaca acaacgcact aatcaattac aacggagtga tgtctgtttt
    40861 acaaccatat atggacaacg gtcagttggt cgtgccatct ggacagacga gtttcagtca
    40921 aatcgctacg cttcgctggg atggttctac ggcgcaagca aggatggata atctgttaag
    40981 cgcaaattat actgaccaaa cattggatgc tgttttgtcg ccatatgatc caataagttt
    41041 aggaatcatt tcgtcattaa aaggcgtggg gtatgggagt gaaagtaaac ctttacctgt
    41101 gattactgga caagatgcga cagttgctgg cgttaaatcg atcattgccg gggaacaaac
    41161 acaaacgatc tttaaagaca cacgtatctt agcaaaaaat acgattgaaa tgatcaaagc
    41221 aatcagtgat ggagaagagg ttccggtcaa tgatactgaa acttatgata atggggtgaa
    41281 aactgtccct acgtatttag caaatacagt atctgtcgat aaagataatt atcaagcaga
    41341 gctgatcgac accgattatt ataaagaatc cgatctgaaa aattaagagg aaccaaacaa
    41401 ggaactaaag agatctaagt cagagagagg ataagaatta tggcggattg tattttaaaa
    41461 atggcagata tcaccaaaaa attttctgga atccgagcgt tagattgcgt gaatctgatg
    41521 attgaacgtg gggaaatcca tgcattgtgc ggagaaaacg gagcgggaaa atcaacattg
    41581 atgaatgtct tgagcggctt gtatccatat ggctcctatg acggggaaat cttttatgat
    41641 ggaaagctgt gtcaatttaa agatttgaaa gatagtgaag aaaaaggcat cgtcatcatt
    41701 catcaagaat tagcactaag tccttatctt tcaataaaag agaatatttt tctcggaaat
    41761 gaacaagcag aaagaggcgt gattgattgg gaactgacag aaaagaaaac atatgaactg
    41821 ctgaataaag tcggtttgtc tatcgacccg aatcttctag tatcccagat tggcgtaggg
    41881 caacagcaac tagttgaaat agctaaagca ttatcaaaat ccgtccgtct gttgattttg
    41941 gatgagccta ccgctgcatt gaatgaagaa gaaagcgcga atttgctgag attgatccga
    42001 gagttgaaag agcaaggtgt aacctccatc attatttcac ataagctgaa tgaaatcgtg
    42061 gatatcgcag atcgaatcac cattttacgg gatggacaga cgattgagac actagaaaat
    42121 gaaatgatca gcgaagagca tattattcgc ggaatggtgg gcagagattt agttgatcgt
    42181 tatcctgaaa gaaaaccgga tattggagaa gtttatttta aagtagaaga ctggacggtc
    42241 tatcaccctc ttgatcacga gcgaatcata aacgaccaca tcaacttgca tttgaaaaaa
    42301 ggagagattg taggaatagc tggactgatg ggagcgggca gaactgaatt cgctatgagt
    42361 ctatttggac attcttatgg aagcaagata tccggacgta tattaaagga aggggcagag
    42421 atatcagtta agaccgttcc ttcagccatt gcaaacggac tcgcttatgt ctcggaggac
    42481 cgaaaatctt taggactgaa tctgctgatg gatattcggg aaaacgtgac gctgagcagt
    42541 ttggataaaa taagtcaaaa aggagtatta gataaagaaa aagaagtaac tgaggcagaa
    42601 aatttccgga aaaagatgcg tataaaaaca aactccatcc atcaaattgt cagtagtctt
    42661 agtggtggga atcaacaaaa agtcgtttta tccaagtggc tgatgacaca acccgatatt
    42721 ctttttttgg atgagccaac ccgtgggata gatgttgggg caaaatatga gatctacacg
    42781 attatcgaag aaatggcagc aaatggcaaa gctgtctgtc ttatctcttc tgaacttcct
    42841 gaaatattag ggatgtgtga tcgaatctat acgatgaacg aaggacgtat tacaggagaa
    42901 gtctcgcgag aagatgcaga tcaggaagtc ttgatgaaat taatgacgaa agaagaggtg
    42961 ggttaagttg gaacaggcaa aaaatcttac ggataaaaaa gagaaaaaac taaatttgaa
    43021 aagtagagca gctgatcttt ttggtcgcta cagcatggtg atcattttag cgggactact
    43081 tattgcgttt caattgatga cggatggtat tttctggcgt ccattgaata taactaacct
    43141 tgttctgcaa aatagccata tattagtact agctgcaggg atgttattag ttgtgctctt
    43201 aggtcatgtg gatttgtcgg tagggtcagt gatggcgttt gttggtgcaa ttgcgggcat
    43261 gatgatggtc aatcgccaaa tgaacccgtg gatgacagtt atcgcatgtc ttgcagtagg
    43321 cgggctgatc ggcgcatggc aaggtttttg ggtagcttat gcagggatac cggcgtttat
    43381 cgtgacttta gcaggattgc tgatgtttcg tggactgaca caagttgttc taggaggcca
    43441 atctctagca ccttttccag ctgttttcca aaaaatgtct acgggttatt taccagactt
    43501 aagcgaagga agcagccttc acttcttgac attgatttta ggaatgatca tcacgatcgt
    43561 gttagttgtt ggtcaatggc gaacaagaaa tcgtcggaaa aatcatttat ttgaagtgga
    43621 atctccaaca gctttcttgg taaaggcggt atttacagca gtcgttgtac taggaataac
    43681 ttatatcttt gcctcttatc aagggtatcc gattattttg atcattcttg gtttgatcgt
    43741 tgcagcatat gggtttttga caaataaaac agtagcaggt cgacagattt atgcgacggg
    43801 cggaaatcga aaagcggctg aactatcagg gatcaaaaca aagcgaatca cgttctgggt
    43861 attcgtcaac atgggagtca tggcagcttt agctggatta gttttagctg ctcgattgaa
    43921 tgcggcaaca cctcaagcag gtacatcgat ggaactggat gcaatggcgg cagtatattt
    43981 tggtggtgct tcgacttccg gcggcattgg aacaatcatg ggtgcgattg tcggtggctt
    44041 ggtcatgggt gtattgaaca atggcatgtc gattttaggc gtcggggtag actggcagca
    44101 ggcaatcaaa ggattgatct tgcttttagc cgttgtgcta gacttgtata ataagaaaaa
    44161 acgaacaacg taggagaacg ccggttattc agaaaaaaga ctgtgacaag acatttgtca
    44221 cagtcttttt ttcgctaaaa gcgaacattc attgtttcgt ttgtatagac taaaaataat
    44281 atatgttttt ttaccattcg tataaattct ctttttgctc ttgatttttt tgcttaattg
    44341 agttaaatta catagggcgc taaaaaaagc cttgattaaa ttaataatgt tcgtgttttg
    44401 ctggatgtga acatcaggag gataatgtaa tgaaagtatt tgattacgaa gatattcaat
    44461 taattcctaa taaatgtatc gtaaatagcc gttctgaatg tgatacaacg gtcacattag
    44521 gtaaacatac attcaaaatg ccagtagtgc ctgccaacat gcagacgatt attgatgaaa
    44581 caatcgcaga attcttagcg gaaaatggtt atttctatat catgcatcgg tttgatgaag
    44641 cagctcgaat tccgtttatc aaaaaaatga aaaagcgtgg attgattacc tcgatcagtg
    44701 ttggcgtcaa gaaagaggaa tattccttta tagagaagtt ggcagaagaa tcgctaaatc
    44761 ctgattatat cactatcgac atcgcgcatg gacatgctaa ctcagttatt gatatgattc
    44821 agcatattaa aaaatatttg cctgaaactt ttgtgattgc cggtaatgtt ggtacaccgg
    44881 aagccgtccg tgaattggaa aatgcgggcg cagatgcaac aaaagtcggg attggtccag
    44941 gaaaagtatg tattacaaaa atcaagacag gctttgggac tggaggctgg caactggcag
    45001 ctcttagatg gtgtgcgaaa gcagctcgca agccgatcat cgctgacggt gggatcagaa
    45061 cacatggcga tattgcaaaa tctgtccgtt ttggtgcgac aatggtcatg attggttcat
    45121 tgtttgccgg tcacgaggaa tctcctggcg aaacaaaagt cgaaaatgga atcgtgtata
    45181 aagaatactt tggttctgct tcagaattcc aaaaaggcga aaaacgaaat gttgaaggaa
    45241 agaaaatctg gatccaacac aaaggatctt tgaaagatac actagtggaa atgcaacaag
    45301 accttcaatc atctatttct tatgctggag gaagagatct ggaagccatt cgaaaagttg
    45361 actatgtcat cgtgaaaaat tcgattttta atggagacac aatctaaaaa ataaataaaa
    45421 tattaggatg ttgtgaaaaa gttgtcctgc attaagtaat aagaacagcc aaacaaaaat
    45481 agttctttat atgttttgtt tggctggaat tattatcgca gatacaagct tttgaacatc
    45541 attattcaga ataagggact gtgataagat ttttgtcaca gtcccttatg ttttgcaaaa
    45601 aaagtttttc ttttttctcg taaattcggt aaaatcaaaa gaaaaaatag acaatagaaa
    45661 agagaagctt atgtgggaaa aagtagagaa aaaacaagga ccgatttatc gtcaaattat
    45721 ggaacagatt atgagagaaa tcgaaaacgg aaggatcaat cctggaagtc aacttgaatc
    45781 agaacgaaaa ctagggcaac tttttggtgt gaatcgttcc accgttgtcc atgcattgga
    45841 agagcttagg gagcttgggg tactaacgag caaacgtggc agcggatggt ttgttaatca
    45901 gactgaatgg gggaaattcg ctgttccaag aatcgattgg cggcaaatga tctctcctcg
    45961 ttatgagcag acggactggt atgagcaaaa aataagagaa gcaaagcaga tccaaaaaac
    46021 atcgtttctt gatttatacg ctagcgagat gccagatgag cttttgccga attttgagtt
    46081 tccttcttat tctcttgaag aaatcttagc ggaagaaaaa gaaatggatc ttttaggcta
    46141 tactccgtta cgtaacaaaa tcaaacggta tttggaaagt aaatttgaat tggcattttc
    46201 ccccaaccag cttcttgtga ctggtggcgg tcagcaagca atctttttga ttttacaaac
    46261 gattctttca gctggagagg ggatagctgt agagtcacct tctttttttt accgattacc
    46321 tcttttcaaa gcttcaggaa cgcgtttatt tgggataccg atggatgaag aagggatcga
    46381 cttagaaaaa ctgaaagaat cgatccataa aaataaattg aaagcagtgc ttatcaaccc
    46441 gaattttcaa aaccctacag gaaaagtgat gagtcaaaaa cgacgagaaa agctagtaga
    46501 attgtgtcga cagtatcggc ttccaattat tgaagacgat gtatttacgg atctttcatt
    46561 tctaaaccaa gaagaagtcc ttcctatacg aacgattgat ccggataatg tgttatacgt
    46621 cggttcgtta tctagggttt taggaaaaac aacgaaaatc ggttgggtga taggacctga
    46681 gacttttatc tcgcaactag ctcaagcgca agaaatgatg gagttttcga tgaatatcct
    46741 tacacaaatc acagtggcta atgtatttaa tgaagcaatt gatttgaaaa tgcaagatgt
    46801 aagaaaaaag ttgaaagaaa aaagccgact gataagcgct tggggagatt cacaatcgtt
    46861 tttcaagctt cattcaattt taggtggtta ctatgcctgg attacttggg atgggaaaaa
    46921 actagacccc gaactagcag aaaaagtagt agatatgggt cttggaattg caccaagtct
    46981 actttttggg caagatataa atggaatcag aatcaattac agtaggctgg atgaaaacca
    47041 aataccgttg ttttattcaa agatgaaaat tctagaaaag tggttaacag aataatttca
    47101 caaagtaaat agattaaaga atataacagc ttggatttaa atttcaagct gtttcttttt
    47161 tttcggaaaa agaatacgat ataaagaaac taagaaaaaa ccattatttg tacgtccgtt
    47221 cacgatgtgt aaataaaata aatgtttgca acaaaactgt ataacaccaa cgtttttaaa
    47281 tagaaacaga tggttgtaag tttgattaga aaatagaaaa aatgcagatg aaatataaaa
    47341 cgctttcctt attgtgattt tattcactaa tgctgttaat atgcggattg gcataatttc
    47401 ctttcatcgt gaaaatttgt gcttttagct tgtatttctc ataaatccat gataatttat
    47461 taatgaagac ggttaaatag tgatacagtt ttattcactg tttcaatcgc agaatgaaga
    47521 gattttctta tagatgagag aggaatgtgt gacaaatggc aaacaaaaaa gcaccgatcg
    47581 acttccaagc actgctagat gaagtcaatg cagatttccc agtatatcaa gcacttgatc
    47641 aagatggaaa cgtagttaac aaagatttag tacctgattt atccgatgat gaactcgttg
    47701 aattaatgac tcatatggta tggtctcgtg tattagacca acgttcaaca gcgctgaacc
    47761 gccaaggacg cctaggcttt tttgcaccaa ctgctggaca agaggcaagt cagctggcta
    47821 gtgcttacgc atttgataaa gaagatgttc ttcttccagg ttatcgggat gtacctcaac
    47881 tagtaaaaca tggtttacct ttatctcaag cttttttatg gtctcgagga catgctgcag
    47941 gtaattctta tccggaagaa ttgaaagctc taccaccaca aattatcatc ggtgcacaat
    48001 atgtacaagc agccggtgtt gcattaggtt tgaagaaacg aaacaagaaa aatgtcgtct
    48061 tcacttatac tggtgatggc ggttcatcac aaggtgattt ttacgaagca atcaatttcg
    48121 ctggcgctta ccatgcaaat gccgtatttt atatccaaaa caacgggtat gcgatctcta
    48181 cacctcgtga aaaacaaact gcagcaaaaa cacttgcaca aaaagctgtc gcagctggga
    48241 ttcctggtat tcaagtcgat ggaatggatc cgcttgcagt atatactgta tctaaaatgg
    48301 cacgggaatg ggcgatcagt ggaaatggac cagtcctaat cgaaacactg acttatcgtt
    48361 atggaccaca tacactatct ggtgacgatc caactcgcta ccgtacaaaa gacacagatg
    48421 acgattggca gaaaaaagat ccattgatcc gcttccgtaa atatttgacg gaaaaaggac
    48481 tttggtctga agaaaaagaa gaacaagtca tcgaagcaac taaagaagaa atcaaagcag
    48541 ctattgctga agccgacaaa gtaccaaaac aaaaggtttc agatttcttg aaaaacatgt
    48601 tcgaagtatc tccacaatca atcaaagaac aaattgcaat ttatgaagcg aaggagtcga
    48661 aataatcatg gcacaaaaaa caatgatcca agcaatcaca gatgccttag cacttgaact
    48721 agaaaatgac gagaatgtcg ttgtttttgg tgaagatgtt gggaaaaacg gaggagtttt
    48781 ccgggcaact gaaggattgc aggaaaagtt cggagaagac cgagtattcg atactccttt
    48841 agctgaatct ggaatcgcag gattgagttt cggtcttgcg ttagaaggat ttcgtccagt
    48901 tcctgaaatc caattctttg gttttatttt cgaagcaatg gacgaagtag tagctcaaat
    48961 ggctcgtaca agatatcgaa tgagcggcac tagaaatcta cccatcacaa ttcgttcgcc
    49021 atttggtggc ggtgttcata caccagaatt gcattcggat aatttagaag gtttgatcgc
    49081 tcagtctcct ggtattcgag tagtgattcc ttccaaccct tatgatgcaa aaggactatt
    49141 gatttcttct attagaagta acgatccggt tgttttccta gaacacatga aactatatcg
    49201 ctcattccga gaagaagtgc cggatgaagc ttacgaagtt cctttggata aagcagcagt
    49261 tactcgtgag ggaacagatg tatctattat cacatacgga gcaatggtgc gtgaagcaat
    49321 caaagcagca gacaatcttg caaaagaaaa tatatctgtt gaaatcattg atcttcgtac
    49381 agttgcgcca ttagatgtag aaacgatcat tcaatccgtg gaaaaaactg gtcgcgtagt
    49441 tgttgtccaa gaagcacaac gacaagcggg cgttgccgcg caagtagttt ctgagatctc
    49501 agaacgcgct atcctttcat tagaagcccc aatcggacga gtttctgcac ctgatacagt
    49561 cttcccattt ggacaagcag aaaatgtctg gttgccaaat gcaaaagata tcgaagacaa
    49621 agtaaaagaa atcgcggaat tttaagaaaa gaagggacga aacaaagtca tggcttatca
    49681 atttaaattg ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt
    49741 caaaccagga gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc
    49801 agtagaagaa atcccgtcac ctgttacagg aacggttaaa aatgtgattg ttccagaagg
    49861 taccgtagca aatgttgggg atgtattagt agaaatcgat gcaccaggac acgaagacaa
    49921 tgaaggggac agtggtgtag ccgcagaatc ccaaactcca gctaaaccgg ctgctgaacc
    49981 cactgttgat acagaatcag ctggatcgtc aagcgaagga gtcttccaat ttaagttacc
    50041 ggatatcggt gaaggaatcg cagaaggtga aatcgtcaaa tggttcgtca aaccaggaga
    50101 tacgatcaat gaagatgata cattactaga agttcaaaat gacaaatcag tagaagaaat
    50161 tccgtcacct gtcacaggga cagttaaaaa cgtgattgtt ccagaaggca ctgtagcaaa
    50221 tgttggggat gtattagtag aaatcgatgc tcctggacac aatagtgcac cgtcaactag
    50281 tgcgccatca gctgaagcac caaaagaaaa agtggaaaca tcaggttcag caagtgtcgt
    50341 tgaagctgcc gatccaaata aacgtgtatt ggctatgcca tctgttcgtc aatttgcccg
    50401 tgaaaaagat gtagatatct cacaagtaac tgcaactgga aaaggtggca gagtaacaaa
    50461 agaggacatc gaaaacttct tggcaggtgg tccaagcagt gcaccagcga aatcagaagc
    50521 gccagaagcg gcagcaccga aagaagcagc tcctgcagca gaaagcaaac cagctgcacc
    50581 agcaaaacca ttcaagtcca acttaggaga cttggaagaa cgtgtagcaa tgacgccaac
    50641 acgtaaagct atcgctaaag caatggtcaa cagtaagcat acagctccac acgtgacatt
    50701 gcatgatgaa gtagaagtgt ctaaactatg ggataatcgg aaacgattta aagaagttgc
    50761 tgctgcaaat ggtacgaaac ttactttctt gccatatgtc gtgaaagctt taacagcgac
    50821 tgttaagaaa tacccagtat tgaatgcatc tatcgacgat gcaaaccaag aaatcgttta
    50881 taaacattac tacaatatcg gtattgcaac tgatacggat cacggactat atgtaccaaa
    50941 tgtgaaagat gcagaccgca aaggaatgtt tgctattgca gacgaaatca atgagaaagc
    51001 aaaattagct catgacggca aactatctgc agaagatatg cgcaatggaa caattacgat
    51061 cagcaatatc ggatctgtag gtggcggttg gtttacacca gtcatcaact atccagaagt
    51121 agctatttta ggtgtaggaa cgattgcaca acaaccaatc gtaaatgctg aaggcgaaat
    51181 cgttgtcggt cgtgtgatga aactgtcatt aagtttcgat caccgtatcg tagatggagc
    51241 aactgcgcaa caagcaatga ataatatcaa acgtctttta gctgatccag agctattaat
    51301 gatggaagga tgataaacaa atggtagttg gagattttgc tgtagaatta gatacagttg
    51361 taattggtgc gggacctggt ggctatgtag ctgccatccg cgctgctgaa atgggacaaa
    51421 aagtagcaat cattgaaaga gagtatattg gcggtgtttg tttgaatgtc ggctgtattc
    51481 cttcaaaagc actgatagct gcaggacacc attatcaaga gtcactagat tctgaaatgt
    51541 ttggagtaac tagtgaaaac gtcaaactag attttgcaaa aacacaagaa tggaaagaaa
    51601 acaaagttgt ccatacgttg acttctggtg ttggtttcct attaaaaaaa cataaggttg
    51661 aaacaatcga aggagaagcg ttcttcgtag atgatcacac actacgcgtc attcatccag
    51721 attcagctca aacttattcg ttcaataacg caatcatcgc aacaggttcc cgtccaatcg
    51781 agattccagg atttaaattc ggaggacgtg tgttagattc tactggcgga ttagcattga
    51841 aagaagtacc taagaaattt gtgatcatcg gtggcggtgt gattggtgcc gaattgggcg
    51901 gagcgtacgc aaacttagga gcagaagtaa cgattcttga aggctcacca caaattttac
    51961 caacttacga aaaagatcta gtaaaattag tagaagatga tttcaagaaa aaaggcgtaa
    52021 cagtcgtaac gaatgcaatg gcgaaagaag cagtcgataa cggtgacagc gtgacagtga
    52081 aatatgcagt ggacgggaaa gaagaatccg ttactgctga ttatgtgatg gtcactgtag
    52141 gtcgtcgtcc gaatacagat gatatgggct tagaacaagc gggtgtcgaa gtaggagaac
    52201 gaggattgat cacggtggat aaacaaggac gtacgaacgt acctaacatc tatgccatcg
    52261 gtgatatcgt tccaggggcg gcattagcac acaaagccag ttatgaagca aaaatcgctg
    52321 ctgaagcaat ctctggcaaa aaagtagctg tagattataa agcaatgccg gcagtagcct
    52381 tcactgatcc tgaattggct tctgtaggta tgacgatcaa agaagcaaaa gatgctggta
    52441 tcgaagcaaa agcatataaa ttcccatttt cagggaacgg tcgggcatta tcgcttggta
    52501 aaacagaagg attcatccgt ctagttacga cgatcgaaga taacgtgctt attggcgcgc
    52561 aaatcggtgg agtcggcgca agtgatatgg tctctgaatt agctttagca atcgaatcag
    52621 gaatgaatgc tgaagatatc gccttgacga ttcatcctca tccatctctt ggcgaaatcg
    52681 tcatggatgc ttcggaatta gcattaggtt taccaatcca tatttgattt gaataataca
    52741 ggcgaaacgc aactgaaaaa tcagttgcgt tttgtttttt agaattatca ataatattat
    52801 tatgtaaact taaaaatatg acaacttctt ggtttattac cacttcctat gctacactga
    52861 agaggaatat gaaataggag agttgaagat gcaagaatat cagtatccac ttgatttaga
    52921 ttggacaaca gacgaaatgg tcattgtaat gaagatgtgg gaagatctgg aacgtgcgaa
    52981 tgaaaaaggg ataaacaatg aagctttttt gaagacttat cagcagttta aaactgttgt
    53041 caaatccatt ggtgaggaaa aacgattggg acgggaattt gaaaaagctt ccggttattc
    53101 tttatatcgt acagtgcagc aagcaaaaaa aaatccaaac aaaactttga gaatgaacgg
    53161 gtgagataaa tggaacagat gattgatcat atcaagtcct ggcttgaaga agctggtgag
    53221 aaaatcaaat cttctcaaca aacattaaca atctcgcaaa aatcaaatcg aacggatcta
    53281 gtaacaaatg tcgatcgaaa catccagaat tttctaatcg aaaaaatcca ttcctttgat
    53341 ccagaggcga aaatattggc agaagaagaa gggtgtaatc aattatcgga tctcgatgga
    53401 agagtcttta tcatcgatcc tattgatggc accttgaatt ttgtgattga aggggaaaat
    53461 ttttgtatta tgctcgctgt ttatgaatcc ggcgttggaa agcttggttt catttatgac
    53521 gtgatgagaa atgaactttt ttggggtgga aaaggaatag gcgtgtataa aaatgatgag
    53581 ccattgccta gaccagcaaa taaatcctta tcggaaggct tacttggtgt gaatagttat
    53641 cttttcggtc ataataaata tggaatccga gaaattgcgg aaagcagcat ggggacacgt
    53701 atgtacggat gcgctggtct tgaactagtg gcaatcttga aaggaaacca cattgcttat
    53761 atttcaaacc ttagcccttg ggattatgca gcgggaaatg tcttattgga agagtttggt
    53821 atggtaacaa gcggtctttt tggtactcct ttgacatttg aaaaaaggga atactattta
    53881 gcagggactt cttgtgcata tgcagaaatc gcagaccttt tgaaaaaaga aggctgaaaa
    53941 aaaggtagaa atcattcaca aaaatgataa aaacgagcaa aagattaatt ttctcgaaaa
    54001 aatgaaaaca ttttactata tttcatgaaa aaagtctgct ataattagca gtcgagtaaa
    54061 atttgccctg tcataaagaa ccaataaaaa aggagttagt tacattgaat tatagaaacg
    54121 atatccgtaa cgtcgcaatt attgcccacg ttgaccacgg aaaaacaaca ttagttgatg
    54181 aactattaaa acaatcagat acattagacg cacatacgca attgcaagaa cgtgcgatgg
    54241 actcaaatgc gctagaaaaa gaacgtggta tcacgatttt agccaaaaac acagctgtag
    54301 actataaagg tattcgcgta aacatcatgg atactccagg acacgcggac ttcggtggcg
    54361 aagtagaacg gatcatgaaa atggttgacg gcgttgtgtt ggtcgtagat gcgtatgaag
    54421 gaacaatgcc tcaaacacgt tttgtattga aaaaagcact ggaacaacat atcacaccaa
    54481 tcgttgtcgt aaacaagatt gacaaacctt ctgctcgtcc tgagcatgtc gtagatgaag
    54541 ttcttgaact attcatcgaa ttaggagcag atgatgatca gttagacttt cctgtgattt
    54601 atgcatctgc actaaatggt acgtcaagct tgtcagatga tccagcagat caagaaccaa
    54661 ccatggcacc tatttttgat acaattattg aaaaaatccc tgctcctgtc gataacagcg
    54721 acgaaccatt acaattccaa gtttcattac ttgactacaa cgattatgta ggtcgtatcg
    54781 gaatcggacg tgtatttaga ggaacaatca aagtaggaga ccaagtagca ttaattaaat
    54841 tagacggaac agttaagaaa ttccgtgtaa cgaaattatt tggtttcttt ggactaaaac
    54901 gtctagagat tcaagaagca aaagctggag acttgattgc tgtctcaggt atggaagata
    54961 tctttgtcgg tgaaacagtt actcctgtgg atcatcaaga tgcgttacct atccttcaca
    55021 ttgacgaacc aacattgcaa atgactttct tagttaataa ttcaccattt gccggtcgag
    55081 aaggtaaatt cgtaaccgct cgtaagattg aagaacgttt gatggcagaa cttcaaacag
    55141 atgtatcctt acgagttgaa ccaacaaact ctccagatgc atggacagtt tctggtcgtg
    55201 gagaattgca tttgtctatc ttgattgaaa acatgcgccg tgagggatac gaattgcaag
    55261 tatctcgtcc agaagttatt gaaaaagaaa tcgacggtgt aaaatgcgaa ccgtttgaac
    55321 gcgtgcaaat cgatacacca gaagaatata tgggtagtgt tatcgagtcc ttgagcttac
    55381 gtaaaggtga aatgcaagat atggtccata caggaaacgg acaaatccgt ttgacattct
    55441 taacacctgc ccgcggtttg attggttatt caactgaatt cctttcaatg acacgtggtt
    55501 acggaatcat gaaccatact tttgaccaat atctgccaat gcttcctggt caaattggcg
    55561 gacgtcacca aggggcgctt gtttcgatcg atacaggaaa agcaacgact tattctatca
    55621 tgagtatcga agaacgcggt acagtcttcg tagaaccagg tacagaagta tatgaaggaa
    55681 tgattatcgg ggaaaacagc cgtgataatg atttgactgt caacatcacg aaagcaaaac
    55741 aaatgacaaa cgtacggtct gctactaaag accaaacatc tgtcatcaaa aaaccaaaac
    55801 aattgacgtt ggaagaatct ttggaattct tgaatgatga tgaatattgc gaagtaacac
    55861 cagaaagcat tcgcttgaga aaacaaattt tagagaaaaa tgctcgtgaa aaagcatcta
    55921 aaaagaaaaa ataatctgta aaaggatgaa gagagaaacc agcttgttat aaaaaatagc
    55981 tggtttcttt ttttgtccaa ctcttttctg tggagaagtc atctttgtca atggacataa
    56041 attcagaaaa tacgccgaaa gacgtttaaa agacttttaa aaaacttatt tctagagagt
    56101 ttttgtttca taataagata atgtttcacg aaaggagatc aacttgtcct tcgaagaaaa
    56161 tcgaagtaaa atggcaagag aaaggagcgg gaagatgaac gacttattaa aacaatactt
    56221 tggctatgat gagtttagac ctggtcaaaa agaaattatc cagaaagtca ttgatcagga
    56281 aaatgtatta ggaatcatgc ctaccggaag tgggaaatca atctgttacc agcttccagc
    56341 tttactatta gatgggctta ctgtggttgt gtccccttta atctctttga tgaaggatca
    56401 ggtggacgcg gcgaatcaat taggtatacc cgccactttt atcaatagct cgttagatgg
    56461 ttacgaaaca gctagacgct tccaagaaat cgatagacag cagtaccgtt tactttatat
    56521 cgctcctgaa cgttttatca tgccggattt tatccaagca atgaagcgat ggaacgtccg
    56581 tatgatcgct attgacgagg cacattgtat ctctcagtgg gggcatgatt tccgcccaag
    56641 ttatttacag atggcgaacc agctagatca actgccaaat cgtcctgtaa ttgttgcttt
    56701 gacagctaca gcgactgtgc aagtagctgc tgacataaaa agacttttaa aaattccaga
    56761 aaacaaccat attcagactg gttttgaacg agaaaatcta cgatttcaag taattaaaga
    56821 tcaaaaaaaa gaacaatatc taatcgaata tttaaagatc aacaaaaatc aatctggtat
    56881 catttatgca gcaacgagaa aagaagtaga ccgtctctat catttgctta aaaaattcga
    56941 tttttccgtg ggtagatatc atggtggtct aaatgagaat gagcgaacag aaatgcaaga
    57001 agccttttta tacgatcgct tgcagctgat tgtagcgaca aatgcatttg gtatgggtat
    57061 aaataaaagc aatgtccgtt ttgttatcca ttatcaaatt cctggctctc tagaagcata
    57121 ctatcaggaa gctggtcggg cgggaagaga tggtctttcc agtgaagcca tcctgctttt
    57181 ctctcctcaa gatatccaag tccaaaagtt ttttatccag cagtcccaaa gagaagaagg
    57241 gcaaaagcaa aaagaatatg aaaaattaaa agcgatgaca gaatacgtgt atattgagtc
    57301 ctgtcttcag caatacatcc taaactattt tggcgagaca agcagtccgt gcaaccgttg
    57361 tggtaactgt ttagatgaca gagaaatagt agaagtaacg acagaagcac agatggttct
    57421 ttcttgctta aaacgtatgg gtgagaacta tggtaaacaa atgctgatga aagtcttagc
    57481 aggatcgaaa gaacaaaagc tgcaagcttt aggttttgga catttatcta cctatggact
    57541 gatgaaaaac caatcgcaaa aagaaacgat gcagttaatt gaatatttga tatcgaatgg
    57601 ctatcttctg accatcaatg gtgagtatcc tgttttgaaa gtaacagaaa gagggataca
    57661 agtattgaaa ggacaagaat ctgtttatcg aaaggaaccc aagaaagtcc agcagctatc
    57721 ggatgaagaa accgatacac tatttgaagt gctgcgggaa ctgcgaacgg atctagcatc
    57781 agaagcaggc gttcccccgt atgtcgtatt ttcagattcc acattgaaag aaatgagccg
    57841 aatacgtcca tcaagcagat tggagatgct tcagataaaa ggagttggcc aaagcaagct
    57901 tgataaatat ggcgaagcct ttttatctcg gataaaaaat gcggactcta acgttctgga
    57961 taagtagaaa gaacgaatac gtaaaaatga ataaaaacaa ctggttaaat ctgttttttt
    58021 tgctccttat gaaaatcaaa tatgttataa ttaaggaatt agaatgggag gtaagcacag
    58081 atggattctt tgtcgaaaaa acttgcgatc caagtattaa atgatgaagc agaacggatc
    58141 aaacgattaa tccgaaatca aaacaacagt ctctgtatct cacaatgcaa agccttcgaa
    58201 gaagtggtag atacacagat gtacggcttt tcccggcaag tcggttttgc tgtccgtata
    58261 ggagtaatcg gaaagaacga gggccagctg atgttaagtg agctagaacg agaattgaat
    58321 cagttatata gtgaggttta ccaagaaatt tatgataaaa aagaagttgg caaggaggaa
    58381 tagacttgcc taagaaagtg aaaaaaaggc atctattaga ctatagcatc ttgattccct
    58441 atttaatttt atgtgtattg ggattgatca tggtatatag ttctacgtcg tatctattat
    58501 tagaaaacgg acaaaatccg tccgcatcag tcattaacca gagtattttt tgggtgctta
    58561 gcttgattgc tatcgcacta ctttataaaa tgaaaacaga tgttttgaaa aaccagcgtt
    58621 tgatcatggc agccatcgca gttttgacga ttttgctgct gattgttgtg ttttttggta
    58681 aggagatcaa tggggcgaaa ggttggctgc aaattgcagg attctcgatc caacctgccg
    58741 aatacctaaa aatcatttct atctggtacc tgtctctgac tttatctaaa agacaaaaca
    58801 gtgtacaaaa aaattttctt gggactgtga aacgtccact tgcaatggtt atcggactta
    58861 ctgcattagt cgctattctt cccgattttg gtaatgcggc agttatcttt ttgattattt
    58921 tggttctgct tttagccagc ggagtcaatt atgtgtatac tttgattgta ggagtaggag
    58981 gcttttgtct aagtactttt acgatctggc tgatcaatat cacaaacgga aagatcttac
    59041 ccggacgttt acagtatatc tataatcgat ttgctattta ccaaaatcct ttctcagacg
    59101 aattaaataa agggcatcaa ctagtcaacg gctactatgc catgttcaac ggaggactat
    59161 ttggcagggg attaggtaac agtatccaga aaaaaggatt tttacaagaa gcacaaaccg
    59221 attttattta tgcaatcgta gtggaagaac tgggagtgat tatggggatt ttgatattag
    59281 cgttgctatt ctttatgatt gtgcgcattg tccttgtagg tatccgttca aaagatccgt
    59341 tcaattcgct tttgtgcatt gggatcggcg caatgttttt gatccaagta tttgtcaatc
    59401 ttggaggaat tactggcgtt attcctttga cagggatcac gttcccattt ttaagtcaag
    59461 gaggctccag tttgctgatg ctttcgattt gtgtcggctt tgtcctcaac atcagtgcgg
    59521 atgaaaaacg caagagctta ggtttataat aaaaaaagat gtaggagtgg ttcgatgaaa
    59581 aaagttttag ttgctaacag gggtgaaatc gctgtacgtg tatttcgtgc ttgtacagaa
    59641 ttaggaatta aaactgttgg aatctatgca gaagaagatg aatattccgt acaccgtttc
    59701 aaagcggatg aagcatattt agtaggacaa ggaaaaaaac cgattgatgc gtatctagat
    59761 attgaaggga tcatttctat cgcgaaagaa tgcggagctg acgctattca tccgggttac
    59821 ggtcttttgt cggaaaactt gaattttgcg caacggtgcg aagaagaagg catcattttt
    59881 gttggaccca aactgcatca tttagatatt tttggagata aaatcaaagc aaaagctgca
    59941 gctatcgaag cggggattgc ttcgattcca ggcacagatg gaccaatcgc ttctatagac
    60001 gatgccttgg agtttgcgaa acaatacggt tatccgatta tgatcaaggc agctctaggc
    60061 ggcggcggac gcggcatgcg tgtagctcac gatgaaaaga gtgcaagaga gggctacgaa
    60121 cgtgcgaaaa gcgaagcaaa agcagccttt ggtagtgatg aagtgtatgt tgaaaaatac
    60181 atcgctaatc ctaagcatat cgaagtacag attctcgggg atacgcatgg aaacgtcatc
    60241 catctttttg aaagagattg ttctgttcag cgccgtcacc aaaaagtagt agaagtagcg
    60301 ccttgtgtct ctatgaatga acagcaacgt caaaaaatct gccaggcagc tgtccaattg
    60361 atgaaatatg tcgggtatgt caacgcaggt acagtagaat ttcttgttga aggagatgat
    60421 ttttacttta tcgaagtcaa ccctcgcgtc caagtggaac atacgatcac agaaatgatt
    60481 acagatattg atatcgtaac aacgcaactg ttgattgctc aaggtttgga tctgcataaa
    60541 gaaatcggat tgcctcagca agaaggaatt aaattaaatg gttcagccat tcaatgccga
    60601 atcactacag aagacccgtt gaataacttt ttaccagaca cagggaaaat cgatacgtat
    60661 cgttcacctg gtggttttgg cgtacgtctg gatgtcggaa atgcttacgc gggctatgta
    60721 gtgacaccgt attttgattc attgctagta aaagtatgta cgcatggtgc tacctttgaa
    60781 acagccattc agaaaatgga acgttgcttg aaagaattta gaatccgtgg agtgaaaaca
    60841 aacattccat ttatgctcaa tgtcatcaca catcctgaat tccaatcagg aaatgcaaaa
    60901 acaaccttca ttgatagtac agcaacatta tttgaatttc ctcgactacg agaccgcgga
    60961 aacaaaacga tgaaatatat cggtgaaatt actgtgaatg gttttccagg tatcgaaagt
    61021 ggcgaaaaac cattttatga agaaccacgt atgcctaaag atctaatcac aagaactgat
    61081 tacgtaacgg ctaagaatgt attagatgcg aagggtgccg atgctttggt cgaatggatc
    61141 aaaggacagg aaaatttgtt gttgacggat acgacattta gagacgccca tcaaagtctg
    61201 ctagcaactc gtgttcggac aaaggatttc aagcagattg cacgtttgac tggagaaggt
    61261 cttcctgaac tcttttcaag tgagatgtgg gggggagcaa catttgatgt ggcttaccgc
    61321 tttttgaatg aagatccttg gcaacggttg agaaaaattc gttctttaat gcctaatacg
    61381 ttgctgcaaa tgcttttcag aggttcaaat gccgttgggt attctaatta tcctgacaat
    61441 gtattggtgg aatttgtcaa agaagcagca gcgcaaggaa tcgatgtatt tcgaatcttt
    61501 gatagtttga actggactcc acaaatggaa aaaagtatcc aagctgtacg ggatactggt
    61561 aaaatcgcag aggcagctat ttgctatact ggcgatatca acgatccaag tcgggcaaaa
    61621 tacaatgttc agtattataa agacatggca aaggaactag aacaactggg tgcgcatatc
    61681 attgcaatca aagatatggc tgggctgctt aaaccgcagg cagcttatcg tttgattagt
    61741 gaattgaaag aaacgacaga tctgccaatc catctgcata ctcatgatac cagcgggaat
    61801 ggaatcatca cttattctgc tgcttcaaaa gcaggagtag acatcgttga cgtggcaatg
    61861 agtgcaatga gtgggaatac tagtcagcct agcatgagca gcttgtatta tgcactggtc
    61921 aatggtccac ggctgccaga aatcacgatt gaaaatgcac agaaattaaa ccattattgg
    61981 gaagatgtgc gcatgtatta caaaccgttt gaaaacggct tgaatgctcc tgaaacagaa
    62041 gtatatatgc atgaaatgcc aggaggacag tattcgaatc tacagcaaca ggcaaaagct
    62101 gtcggattag gtcatcgctg ggatgaaatc aaacaaatgt atcatactgt caacttgatg
    62161 tttggcgata tcgttaaagt aactccatca tctaaagtag ttggagatat ggcactattt
    62221 atggtccaaa atgatcttac agaagaagat atttatgaaa aaggcgaaac actaagtttc
    62281 cctgaatctg ttgtgacttt cttccaagga gaactagggc aacccgttgg cggatttcct
    62341 aaaaaattgc aaaagattat tcttaaaggc agacctgcat tgaatgaacg tccaggtctt
    62401 ttggctgaat cagtcgactt caacgaagta aaaaaagaat tggcagaaaa aattggctat
    62461 gaaccaaaac aagacgaagt attaagctat ttgatgtatc cgcaagtctt tttggactat
    62521 caaaaagcat ataatcaatt tggcgatgtt acattactgg acacaccaac atttttccaa
    62581 ggtatacgtt tgggtgaaac aatcaatgta caaatcgaac gcggaaaaac gcttatcatt
    62641 cgtttagatg aaatcggaga acccgatatc gaagggaatc gcgtactgtt cttcaatttg
    62701 aatggacaaa gacgtgaaat cactatcaaa gataattcga tcatcagtac ggtccaaacc
    62761 cgattgaaag ctgaaccgac aaaccgagaa caaattggag caacgatgtc tggttctgtt
    62821 ttggatgtat tagtgaaaaa aggcgataac gttaaaaaag gtgatacgtt gatgattact
    62881 gaagcgatga aaatggaaac ggccatcgaa gcacgttttg acggagaagt cgcacatgta
    62941 tatgtgtctt caggagacac tatttcgtct ggtgaccttt tgattgaagt gactgagaaa
    63001 taataagcga aaggtgggga aacatgaaaa gactgggttt gttttggctg attctgctat
    63061 tagttattgt ggcattctac atggaaccag tcttgtttcc gccaaaactt gaagtgaagg
    63121 aaaactctga tcaacaaata tcaaaccaaa tgacaacgtg gaaataccag gagcttaaag
    63181 caactggttt ttctacttat atcggaaaac cgatcgatca gctggaaaag gattttggcc
    63241 aagcctatga tcgattaact agcggatttg gttttgaaac aagatattat gaaaataaag
    63301 ctagtcatct cacatttgaa gcgaacatcc aagacagcag agtcacaact gttaaagtac
    63361 tgcaaacagg gcctgctgat atcgcgccgt tttatttagg gatgacgatg caagatctta
    63421 cgaaaatcac tactatctat actaatttca cttttgagta ccatgataca gaagttggaa
    63481 tcgaactaat ggaagaggat atgaattatc gtccgctaat tgcttttgat aacgacactt
    63541 ttgctatttt attttttgat caaacaacaa acggcctatt ttcagttgtg tatttagata
    63601 aagatagtct tttgaaacta ttgccatatc aagtgtttgg tgaaggcctt ccacattatc
    63661 aagcagataa atcggctgat tgggaatcaa tcaatcgcgt taaagaacga aaaagtacga
    63721 ctttattaaa tatgcttaga agagaagacg aacttcccat atacaatcaa acacttgcct
    63781 ttcaaaacca cagtcagctg ctactacatg attttttgac aaggcctgag gaaatattgt
    63841 cagaggaacg tatggctgat tggcaaaaat cgctaggcag tgctcaaacg acagtgaaat
    63901 tctcactgac gaatgatgaa ttggagaagt taatcagtaa acaaaaatta gaaaatacta
    63961 gtgggctatt tattcatccg gcgattgacg ctactttttc gtttttatat tggtacagcg
    64021 atccttacaa ccatgaccgt ttcatggacc cgggaacgga ttctgtaggc attgcttttt
    64081 ctaaagaaaa tatgttagtc ttattgcaag aagaaaacgg agaaagtagt gatagcagtg
    64141 atcgttaacg aagagctgtt tcaactggag gatcaatgcc tccaagtcgc tgaaatgata
    64201 aaaaagagta aaacgatggc ttcttatctt gaacataaac agtcaatgga agaatctgca
    64261 aaagtttgcc agttgaaaga agcgttcttg tctaagaaag aagcatttga aaatgtagcg
    64321 gcatatggtg agtatgcacc tgattatcgt gaaaaaaaac gagttgttcg aaaagcaaaa
    64381 agacagcttg atatggatga gcatgttgca gctttccgat atagtgaaac acagctgcaa
    64441 agtctgttgg atcaaattgt ccaagtactt gccgaagaag tttctaatga aatcaaagta
    64501 gatgcaggta gcccgttttt tgaaacgaaa aaacactttg gatgcggagg taattgtcat
    64561 gcaattagac aatagtgaat ttgaaataac aaaacgacgc gggctgatcg tatgggtcta
    64621 cagcttaaaa caattaaaga atctgaaaaa gttcggcttg ttgcattatg tctcaagaag
    64681 aatgaaatat gtcgtgcttt acttaaatga agagacattg gaagaaacag aagaacgaat
    64741 ccgtaaatta cattttgttc gaaacgtaga acgttcttac cgaccggata tcgaaatgaa
    64801 ttttgccgaa aaaataggaa acaaaacagt attaaaagac gaaggtttcg aaattgaaga
    64861 gctaaataca gaaatccgat tagctgaaca tctttaaaaa tctcttatga gcagttttta
    64921 aataaaaaat aacttggata acgatcgatt tttgatcatt gtccaagtat ttttttggta
    64981 tactagtacc agtaccattt ctaaggagtg agtagatgcg gataatatca ggaaattatg
    65041 gcgggcgccg tttaaaaagt ttagcagggg cgaacaccag accaactaca gataaagtaa
    65101 aagaatccat attcaatatg attggaccat attttgatgg agagacagta ttggatctgt
    65161 ttgcaggaag cggtggttta gctatcgaag cagtatctag aggctgcagt catgcggtat
    65221 gcgtagacaa aaattaccaa gcgctaaaaa tcatcaaaga aaacatcgaa attacgaaag
    65281 aaccagagaa atttaccaca ataaaacttg atgcggacaa agcaatcgaa cggctagcag
    65341 cggagaaaca acaattcgac tacttgtttc ttgatccgcc atatgcaaaa caaaaaataa
    65401 tcgatcaaat ccataaaatg gaagaactgg gacttttttc taaaaccgca gttatcgtat
    65461 gcgaaacgga taaaacagtc gaattgccag aaactgtggc tggattccat caaataagaa
    65521 aacagattta tggcataacg gccgtaacta tctatagaaa agagggaaat caatgaaacg
    65581 tgcattattc ccaggaagtt ttgatccttt tacaaaaggt cacttagata cggtggaacg
    65641 tgcggctaaa ttattcgatg aggtagtaat tggtgttttt atcaatactt caaagaaaag
    65701 tttgtttccc ccagaagaaa gaatgacatt gatcacaaaa gctgtttctc atcttcctaa
    65761 tgtaaaagtc atgcatcaag aaaatcaatt gacggtagaa acagcaaaag agattggtgc
    65821 agatgcgttg atcagaggca tacgaagtat caaagacttt gaatatgaac gagagattgc
    65881 tcagatgaat catcatctat atgatgaatt ggaaactgtc ttcttattag caaaaccaga
    65941 atatagtcac gtgagttcca gtattttaaa agaagtactg cactttggcg gagatgtgag
    66001 cagctactta ccgccagtga tcaacgaagc tttagctgga aagcgtgaat caaatgaaag
    66061 ctaaacaatg gttgaaaaca gtactgatta tcatatttgg aatgttgatc attggatttg
    66121 tagtagttcc cttgccgtat tatgtagaat caccgggagc cacgatcgat ctgaaagaac
    66181 taattacggt caatgataag gaagataaag gttctgggtc tttctctctt acctctgttg
    66241 gtatcaggca agctacaggt ttcttagctt taaaagctaa aatgacccct tttgaagaat
    66301 tgatcagtga agaagaattg actggtgggg cgacaaatga agaatataat caaatgcaac
    66361 agtattatat ggattcttcc caaaatgcag caatcgaaca agcgttaaaa cttgcagaca
    66421 agccttataa aatggaattc aaaggcgtat atgtcatgag tgtagagaaa acatcgaatt
    66481 tctttggcaa gctttcagtt ggagatacag taacaaaagt agacgggaaa agctttcaaa
    66541 gtacagaagc atttatggat tatgtaaaaa gccaaaaagt tggacaaaca atcgaaatca
    66601 gttatttgca taatggtgaa gaaaaaacag cttctggtga tctgatcgag ttaccaacag
    66661 ataaaaaggc aggaataggt atcacgctta ctgaccatac cgagatcgaa tcagatacag
    66721 atgttcgtat taattctggc tctatcggtg ggccatcagc gggattgatg ttcactttag
    66781 aaatctatga acaagtcact ggtaaaaact tgcgacatgg taagcaaatt gcaggaactg
    66841 gaacgatcaa cagtgaagga gaagttggcc gaatcggagg aatcgataaa aaagtggcaa
    66901 gtgcggataa agctggcgta gagattttct ttgcaccaga tgatacgatt tcagacgatg
    66961 ttaagaaaga atatcctgaa ataaaaacca actacgaaga agcaaaagct gccgctaaaa
    67021 aattgaattc ctcaatgaaa atcgtaccag taaaaacagt acaagaagcg cttgactatc
    67081 tagagaaaat gaaatgaaac taaataatta aagaggggtt gtgacacgag ttctgtcaca
    67141 acccctcttt ttcgtcaaaa atcttcacga taaaaataaa aacaaaagaa aaaattgacg
    67201 taattagatg ttgaagggac gattttgcca gagctcactg tttcttcaat tttaaatgaa
    67261 aggagtaatg aatgattttt actggtcatt ctttgaaaaa agaacaatga tagaaaaaat
    67321 cagcaagaaa atactagctt cacccaaatt gatcatttgg atgtctatga tagttcttat
    67381 cttttgtatc gcaagtggtt ttttattatc aaatttgcta ttttatagag aaacagaaga
    67441 gactgctgca aaagaaatac tttcttcgtt tagttccagt ggagaagatc catcagctga
    67501 aggaaacgat gtagaagagg atgctcgtcc aaaggtcatg tacaccgata tcaaaggaag
    67561 tgtgaaggag cctggtatct attccttttc ttcagaagaa cgggtctatg atgtactaaa
    67621 gcgtgcaggt ggattattag aagaagcgga tagcgatcga atcaactttt ctgcgaagat
    67681 agaagatcag caagtcctat atatccctgc tgtaggtgaa gagctgccag aacatcttga
    67741 tcagttggca tcctcggaag gaaaacagcc tacagcagat tccgagcctc ccaaaataaa
    67801 tatcaataca gccactccat cagaacttca gcagattcct gggatcggat cagtcaaagc
    67861 gcaagaaatc attcgattta gagaagaaaa cggatccttc cagaaagtgg aagacctgca
    67921 ggagatttct ggtatcggtg aaaagacagt tgaaaaacta aaaaacttcg ttacaattaa
    67981 atagaatgat tcaaaatcga ctggcacgag caataaatgg acgctaatgg atactaagaa
    68041 taatccaatt ctaagagctt ggcaggacga ggaggtagta tttttggata ggaaatagct
    68101 tgattgttcg caaagaaaca ttaaaaaaac caatgctgaa tgtctatctc caaaataaaa
    68161 tatctgggat tcatattatg aatacggctg tcagcggcaa taacagtcaa gctctacgag
    68221 aacgctttgc caaagatgta cttagctata cagcagataa agtatttatt ctcattggga
    68281 cgaatgatct agcagagcat aaacaacttt ctaaagaaac gtaccaaaaa atctgcagtg
    68341 gatgatagtt cagctgaaaa aagactatag ttctgatcat gctttcttta tcacgccacc
    68401 agcagtcgat gagacaaaac aaaaatatcg gaataacaag ttgatcaaag aatatatctc
    68461 tgttctcaca gaagtagcaa aacgccatga ttgtgaagta tcgattttta tacggaattt
    68521 cttgaacaaa tggattcgaa gaatttttat aatgggctaa tggatgacgg acttcatttc
    68581 ggcagtgaag ggtaggatct tttgtcagac aggatgattc gttatattta aagaatattt
    68641 tgtttaagca atgagtactt tttaatcaac ttgaaaaaac ggaggaatat aaaacatgtt
    68701 agaaaaatct tcaaaagcat gcgatacgaa taaagaaaaa gttactgata agtcacataa
    68761 tgaacgcatt ccttgggacc agtactttat ggctcaggca gtactgttat ctttacgtag
    68821 tacgtgtaca cgtttagaag taggagcgac actcgttcga gaaaaaagga tcattgctgg
    68881 cggctataat ggagcagtca gtggagatgt ccactgcata gatgaagggt gttacgtcgt
    68941 ggatggacac tgcttgcgaa cgatccatgc agagatgaat gcactcctac agtgtgcaaa
    69001 acttggtatc ccgacggaag gttcagaaat ctatgtcaca cattttccat gtttggcctg
    69061 tacaaaggct ttgttgcaag ctggtgtgaa agggatcaat tatctccatg actatcgaaa
    69121 tgatccttat gcccaagcgt tgatcgaaca attaggtgta tctgtacacc aagttgcttt
    69181 agactcacag catttttcaa aacttcaaaa tgaattaaca ggaaaacatg tctagcgttt
    69241 tagaaaggca tgttcgaaat cggttgattt ttccggcaat tacgtcgatg attatctgtt
    69301 tgttgtatta cactgacaat tggggttatc gtttgcttct tgccggaatt tttttatggt
    69361 tttcgctaaa agaaggacgt tttatcgcgt acttgtgtat tttgtgtagc ataacggtat
    69421 ccatttctta taggctacaa gagtattcat ttttagagga aaaaacaaca tctgcgcaaa
    69481 ttactgtatt tacagatacg attcgtgcaa atggtaattt tttgacaatg gaaggaaggt
    69541 tatcagagaa taaacaaaag ctccagcttt cttatcaagt aaacacagaa gcagaattga
    69601 aaacatggct taacttccga ggaaagggaa tagaattgac tgctgaaggc gagttcatct
    69661 ctccagcaaa aaagcggaat ctttataatt ttgaccaaga agcttacttc aggaaccata
    69721 gaatttctgg acggttccaa attcaaaaga ttgaacagat caccatgcaa aatcactggc
    69781 gaaactggct tcagagaaaa agaggatctt ttatctcctt tgtccaatct gaatttccgc
    69841 aaaaaacaag tgtatatatt aattctttat tatggggcta taaagatgca ggattcagag
    69901 aaccagaaga aatttttagg gagagtggtt tgctgcatct gttcagtcta tcgggtctac
    69961 acatccaatt ttatttaggg tggctgtatt atttatttcg acgtatcgga tggccactac
    70021 acgtcagtat tattccgttg agtctactaa ctatctgtta tgtttctatt gcaggaagtt
    70081 ctatttctgt tttgcgtgct agtttgctgt ttttattgcg attgctagtc aaattgctga
    70141 ataagcatta ttccaccatg gacttatttg cagttgtctt ttggcttctt ttactatttg
    70201 atcctcgatt tctttttcat gcgggtggac agcttagttt tttcatgagt tttttgttta
    70261 tcctgatttc ttttgatgga gattggttga aaaaaacggc ctctcacgtc ctgtttacta
    70321 tgatcacgat gccgttgatt gtatggcatt tttatgagtg gcctatttta ggtagtcttt
    70381 ttacattact gattgcaccg gtattcaagt ttctttttct tcctggtgtg ttatttctca
    70441 cactcttcaa tcgctggctg ccggaaactt tacttttgct gatagaagag ggattaatct
    70501 tatttgagcg aataatcgat ttttcttcag ggtcgtctct cattatagga cgtttgcccc
    70561 ttgtgctatc tgggatgtat attttcggat tactgatttg tatggatagg ttgaaagaac
    70621 aacctgtgaa acacttgttt tatgttggtc ttttttcttt tatttttttg actttgccat
    70681 ttttacgctt ttcttcaacg atcgctttta tcgatgtcgg tcaaggagac agtatctttt
    70741 tgcaaagtcc ttttcgaaaa gaaacgattt tgatcgatac tggaggtcag ttacactttg
    70801 aaagagaaaa gtgggcgcaa ggtatgatac gtcctcctgc tgaaagaaat atcgttccat
    70861 atctaaaagg tcaggggata agtgtaatcg ataaactcat cttgacacac gacgatacgg
    70921 atcatgtggg agaattaccc acgttatctc agcattttac tatcaaaaaa atttatatcg
    70981 gttggggcgc tggacgaaat gagccattac aaaggcattt atctgaagct caaaaaaatg
    71041 gtactgagat cattgaaatc aaacatgggg acaggatcag cggttattat gatctttatg
    71101 tattgactcc ttttgaaaag ggagagggaa gaaatgaaga ttcgattggt ctatggatgg
    71161 aatataacaa taggcgcttt ttatttttag gagatttgaa tcaagcaatg gaaaaacaat
    71221 tgcttttgat ttatcctaat ctgaaagccg atgtagtcaa attaggtcat cacggtagcc
    71281 gtacgtcaag taacacagat tttctttccc atattgaagc aaaacatggg attatatcct
    71341 gcggcgtaaa taatcgttat ggtcacccgc acagcgaagt attggagaca ctagaagaaa
    71401 atcagataaa aacatggcga acggatcaac aaggaatgat cacctatcgc tggcatccag
    71461 tttttcatcc tcatgggcta gtggaaacaa tgatagacta gtaattctca tctgctcatg
    71521 gtaggataat ttttgaaagt ggtgagtaat ttgagtatac aagcagagtt acaaaaaata
    71581 cgtaaagaaa aacttgcatc ttgttacttg attttaggaa cagaaaagtt tctccaagat
    71641 caagttcgga cagaaattgt caaaaaaatc caattagagg gcgaagatga cttaaatttt
    71701 ttaagttttg acatggaaaa tgcaagttta gatgaggtag tagctgaagc agagacgttg
    71761 ccgtttttcg gagatcaacg gttagtattc gtagagaatc cttatttttt aacaggtgaa
    71821 aaagtaaaca atggaatcga acaaaatact gatttattga cggcttattt gaaagaacca
    71881 ttagaatcga ctgtattagt attctttgcg ccatatgaaa agttggatga acggaaaaaa
    71941 gtaacgaaac aattgaaaaa aacagcaatt atcatcaatg tccaacagtt gaatgaaaaa
    72001 gaagtacgtc aatatctgat gaatacctta gagaattcct caatcaaaat ggaacgaagt
    72061 gcaattgatt tattcttgag gttgaccgac ttagatttat ccaaaatgat gagagagtta
    72121 cagaaattgg tcctttatgg acaaaatcaa cagcaaatca cggtaagaga agtagaagaa
    72181 ttagttccta aaacgttaga acataatatt tttgacatga cccaatatat tttaaaaggg
    72241 aagacggaac aagctttgcg tttatacgaa gatttagtta tgcaagggga agaaacaatc
    72301 aaaattaatg cgattttgct ttctcaatta cgtttatttt tacagacaaa atttcttgta
    72361 aagattggtt atcagcaggc aaatattgct gaaacattga aaatccatcc atatcgtgta
    72421 aaacttgcca tgcaggaagt aaaaaaattc gatgaacatt tgctggtaca gttgtttgat
    72481 caattagttg agatggatta tcaaatcaaa acgggacaaa tcgaaaaaga attaagtttt
    72541 caactattcg ttttgcgcac agggcagttg ttaagaaatc gataagaaga aagacgaggg
    72601 caaagcatgc gggaaggtcg tttgtttttg tccttttttt gacaaataaa aaaatcggcc
    72661 gaagccgatt cttttattta gcgatttttt tgcttaaacg agatttgtcg cggttagctt
    72721 tgtttttgtg gattagacct tttgattcag ccatatcaat agctttaaca gcttctttat
    72781 ataacgcatc tacgttgtca gcacctgaag ctacagcatc ttcaaatttt ttgattgcag
    72841 tacgcatagc gcttgtttga gatgaatttt tcacattagc gttttcgctt gtacgaacac
    72901 gtttgattgc agattcaatg tttggcatta gtttcacctc cgataactta agttcttacg
    72961 cgtgttaccg cataattcaa cattcattat tatacctaaa ggcagcgtgc tttgcaatat
    73021 gaacatgaca agtttttttc cttaacagtc ttttattagg attttttttc agtaaaaacg
    73081 gatagtacaa gctgaaaaaa atcaaaaaga cgctattgtg ggaggcaata gcgtcaaacc
    73141 aaactagatt tttatttatc tatttaaaca aagtttgaat gggaatgttt ggtactaagt
    73201 tattatataa caaagagaac cgtttgcaaa gttttttttg aaaagggata cgattccctg
    73261 ttgttctttg tttttctatt aagaaactgc agttatttta taaccagtat cctttgatca
    73321 gtaaaggcta ttctggtgta ccgattattt taatgtattc atcgatattt tttatcaaca
    73381 ttttttttat gaccaactgg ttccgacggt tccatttgcc acttacttcc agcacatatt
    73441 tatttagggg caaacgtaag atatattttg ccaaatagcg atctttaagc aggccattga
    73501 tcacacggtc ctcaagctgc aagttgatcc cagtcagcat cattggctgt tcttgtaaaa
    73561 cgtacgtatc aattaaaaga ccttcaaaag tttccacaag cgtcttcctt tcttttcaaa
    73621 gtaacctgaa aacaggcact aattcttcca ttttgatatt cgttgaagtg gatgcgatca
    73681 ttctttcttt tgtccactgt aatctagtat aacaaaaaag agcagaagag ccaaaaagaa
    73741 aaaagagcat cgaattctat aggaagtctc attggcacgg cctaaattat tacaaaacat
    73801 tgattaaaaa aagcccctgc atcgcaggag catcccctat atcgttttaa taggtcaatc
    73861 aaaaagcagt atcaaacaca ttttgtatca aatcaggaaa taatagataa gctaagccca
    73921 gaatcaaaag taccgctaga agaaaccagc cgattgttcc tatgaaatac tttaattttt
    73981 cgaacattgt atcacctcgt tgtcagtata tcacggatca gtaaattggg ggtgttaaga
    74041 atctcctaaa atagacaaaa tttgttctat tttcctaaag caaatacttg taagatacag
    74101 taagtgatac cagaagcaat gaccggtccg gcggcgattc ctcttaagaa gacaacacct
    74161 aaaatcgtgc cgaaaacaag tgcaacagtg atttgtggat cagaagcaat caatcctaca
    74221 cccttggaag ataagacagc cactagccca ccgcaaaaaa cagctaccca gccaataggg
    74281 gatttaaagg cttctaatag atcacgtaga ccgatttctc ctgttgcgat agggattaga
    74341 atagccgctg tgatgatcgt caccccaata ttgattcctt tgccttgaag ccaagggaaa
    74401 aaggaacggg aggctggtat gagcttcata atcaaaacaa aagctgccgc tatcatcaga
    74461 ctttgatttt tagctaaaat tgctacaagt aaaataattc ctaaaaaaag ccaactttcc
    74521 atgaaatata ttcctcactt tcttcgaaac aatcataaca tgtttttaga aagttacaaa
    74581 agtaggaatt catccttcaa gacctattca taagctgttt ttttcataaa ataaacaaaa
    74641 tcataaaaaa acactttgct tatttgacag aagacagccg cgctttgtat agtggacaag
    74701 acttacattt ttttgaagaa tcagaaagat caggtaaagg tcttagtaaa cgcaagccat
    74761 tcagaataac caatattgtg cttccttcgt gtcctacaac tccaagaggt agatttaaaa
    74821 attgcaggaa gttagaagca atcaacaaga gaataacaag aatcgaaaaa ataatatttt
    74881 gtttgatgat ccgccgcatt tttaaagata gccgatgact gtagacaaat ttatttaagt
    74941 cgcttttcat taagacgatg tctgcgatat ccatagcggc atctgtccct tgccccatag
    75001 cgacacctaa agaagcggct gctaaagcag gcgcatcatt gactccatca ccaaccatcg
    75061 catttacttg gtaaagttca ttctcttttt tgactagttg tgttttttct tccggagtac
    75121 agcttgcata gtaatgttca atccctactt gttcagcaat ggcctttgct gtgccagaat
    75181 ggtcgccggt tatcatactt gtatggatgt tttgggattt aaagtaggaa attacttgtt
    75241 gggtatttgc ttttggaata tctagcagac ctaaaacagc aattaactgc tgatctttag
    75301 aaagataaat cacggttttg ccttgatttt ccaaacgttc gatcatttcc gcaatctctt
    75361 tgctgattat catcgtctca ggatcatatg cgtgcttgcc aactttccat tgcgcaccta
    75421 aataggtagt ttgtaatcca aaaccagtaa tttcttctat tggtaagtat tggatttctt
    75481 caggtattgt gcaatcgaaa tattgcaaaa tcgcatgagc aagtggatgc gtcgtttttt
    75541 gttccatagc aacaagaagt tgttgtgcca aatccttttc atcaaagaaa aagctgtcag
    75601 ttacaactgg ttttccttta gtgagcgttc ctgttttatc aaaggcaatc gcttttaatt
    75661 cagaaagctt ttctaaatgg ataccgcctt tgattaaaat cccatttttt gcagaatttg
    75721 aaagagcagc tagagaagct ggtgtagcag atgcgacaag cgcacaagga gaagccacaa
    75781 ctaataatac cattcctcga taaaagcttt catcccaact ccaacctagg aaaaagtggg
    75841 ggagaaggat cattagagga acagcaagca aaatcaattt tacataatta ttttcgattc
    75901 gttcgataaa tgaagctgtt tgagttggcg tattctgcgc ttcttctact aattggataa
    75961 tcttagcaaa gacagtttcg gaactcgttt ttgttactcg aatcgtgatg ggatttcctt
    76021 gattcagcgt tcctgcatac atctcatcgc ctaaatattt ttctacaggc acagattcac
    76081 cgcttaatgc agactcatcg atcgttgcct gtgtgctttc taaaaagcca tctatcggaa
    76141 cagctgctcc tttagcgaca aataccaagt tatccacctt tagttgagaa acatctactt
    76201 cttgcatttg tccattttct tttaataatt gggctttttg tggttgaaga ttcatcaaag
    76261 cagtaatttc tttttgactt ttattagttg tgtattcctc taatgcaccg cttaaacaaa
    76321 agatgaatgt caacattgct ccttcaaagt aattaccgat caaacaggca ccaatagcag
    76381 ctaaagccat caataaatcc acattcagtg tccgatcatt ccacaattct ttcaagcctt
    76441 ccgatgtttg tttatatcca ccgatgataa tggcactgat ataaaaaagt ggggtgatgg
    76501 taagagagaa tcggtcgaag ataaaaccga agatcataaa taaaaggcag ccgattgttg
    76561 ctgcaaaagc ttttttctga ttagtcatcg ttattagacc tccttgtcaa aacaaggata
    76621 gcataataaa aatgtaattg ataataataa cagctaaatg agaacgataa tggttatcat
    76681 ttggtgatta agcttgatgt tgtttttatt atttataatg attattatta aagaaaataa
    76741 aaaaccagaa catttctcaa ataagagaag tgttctggtt ttttctacat tgcaataaac
    76801 caataaccaa tcaaaatgat accaaggata atacgatacc agccaaaagc ggtaaagtcg
    76861 tttttcttca aatagcttag taagaatttg atagctaaga cggaaaccac aaaagatacg
    76921 aatgttccta caagaagaac aacagtttct tccattttga atgaattccc ttcagctaaa
    76981 tatttgacga tcttcaagcc gcttgcgcca aacatgacag gaatccctaa gaaaaatgaa
    77041 aactctgtag cgacaaaacg cgaagcacca atcaagatag atcctaggat cgttgcgcca
    77101 gaacgggatg ttccgggaac aagtgccaag acttggaata acccaacaag aagagctgct
    77161 tggtacgtaa agtcattcag agaagtccaa cgaggtgttt tgtttttgtt gcgtttttcg
    77221 acaataataa aggcaatacc gtaaacgatc aaaacaatgg caactggtaa gaacttgtgg
    77281 aaatttgctt ctaaccaatc atccaaaggc aaaccaatga tgatggctgg tatgcaagca
    77341 acgatcactt taaaccaaag tgtccacgta tctttttttt ctaccgttga tttacttgga
    77401 gaaaatgggt ttaacttgtg aaaataaaga acgacgacag ccaagatcgc ccctaattgg
    77461 ataacgacat tgaacatcga cataaattgt gaactggcat ccagtttgat gaattcatct
    77521 gctaggatca aatgaccagt actactgatg ggcacccatt ccgttattcc ttcaataata
    77581 ccgagaatca ctgctttgat aatatttgca aaaaacataa attccttcct tcccaattaa
    77641 gatacatctt atagtatacc gttatttttt gagaaaccca atctgttttt taatattaag
    77701 aaaaacttga gaaataaagc agataagtac acgaagagaa aaaagaagac aaagccctcg
    77761 ttcagacttt gccttttatg attctctggt aaaaattaaa atatccccat tatagaaacg
    77821 agtcgcgact atgtgttttc ccgttatcga tataagacgt atcattgatg ccgtggatag
    77881 aaaatcgacc gtttgcataa gtgacctttg ttacgctgcc atttaaaagc ccagcacgaa
    77941 caggctgttt aggatcgatc agattaagca aaaaggcgat cgtcaaacca tgagatacga
    78001 ctaaaacatt tccaccacca tttttttcgc agtgatgggc gatatcttca aatcctgtcc
    78061 atacacggtc tcgcaatgtt tcataagttt ccgcccaccc agcggtatct aagttgtata
    78121 cagcattagc aatttgttca aaagaaactt ctgtggtaaa catttcatca tatgttttaa
    78181 aattcaatac acgagggatc acaccccaca tttccatgtc atatccacct tcaagagacc
    78241 cgaaacacca ttctctaatc cttttttcaa taaaataagg aatttctttt cccttagaat
    78301 gttctcctag aataatacgg gcagtttcga ttgcacgacc actgtcacta gaataagcaa
    78361 atgaaaagtc gacatttttt aacccgcgac ctaaataatg aatgccttct tgtccttgct
    78421 ttgtgagggg tgtatcacac cagccttgta ctcgttcaat tgtattgaac atcgtttttc
    78481 cgtgtcgtac gatataaagt gtagtttctg tcattttatt ctcctcctaa gtcgttttaa
    78541 cgattgaaaa aaggatttgt ccttttttcc tgttcaatcg tcgttgcatc gccatgtcct
    78601 ggatagactg caaattcatc aggtaataca aacagctggg tccgaatact atgaagtaat
    78661 tgttctaaat tccctgtata cagatcggtt ctaccgatac ttcctctaaa cagggcgtcg
    78721 ccagaaacga caaaatcatc aaaaataaag ctgacgcttc ctatagaatg ccctggggta
    78781 gggacgacgc gaaaagacat gtttcctaaa gtataagttt tcatttcaaa ttcatattct
    78841 gcaggcgaga caataacatc agcaatatca tcatgtctac cgagtccaga aagattcaaa
    78901 atcggatcac ctagccattt ttgctctagt gggctgacat aaactgggat ttgataatac
    78961 gcgcgtaaag gttccactgc accgatatga tcgtagtgag tatgggtaag caaaatcgct
    79021 accggctgtt ggtttgtttt tttgatttct tgtttgattc gttccgcatc ttctccaggg
    79081 tcaatgatca aaagtgcttc ttcgttgtag accaaataac agttttcttc aatcattcct
    79141 gtttttaatc tttcgatatg catgtgcatt cctccttgat agccaaaact tctttcatcc
    79201 attagtataa cgcattcatt gctaggaaac aaaaacaggt acttatttct ctttacttat
    79261 ctgaattatc agactatatg aaaagtattt ggatattcta aagagaagga aagtaagttt
    79321 tcatagcatt ttaatatgtc cttgttacat tagattcatc caaaaatcca aattctatat
    79381 cctatatccc tgcctaatca gcagggtgtt tttttatgtt tggaaagaga gctctttttg
    79441 ctaaactagg ttttttcagg taatcttaga gagaaagagg tggtggaaat gatcaaacag
    79501 tcaaaaatat ctgtgcggca cttagtagaa tttattttgc gacgtggaag tatcgacaat
    79561 cgtaaaaaaa gtaaccatac tgcattagaa ggagcgagaa tccaccgcaa gctccaaaaa
    79621 gatgcgggag aaaactatga aaaagaagtc tttttaaaaa caacagttca attagatgaa
    79681 tattacctga tcgttgaagg ccgtgcagac ggtattttca aaagagacgg tatttattat
    79741 atagatgaaa tcaaaacctc cgaaccacga tttgaagatt tagaaccaga acaagtggaa
    79801 cttttctttc atcaagcaag ggtgtatgcc tatatctata gtcaagaaca tgatttacaa
    79861 gaaataaatc tgcaattgac ttacttccag acggcagaag aagtgatcac gagaaaggtt
    79921 gaacatcaga caagagagca gttagaaact ttttttaaaa agctgactga agattataaa
    79981 gaatggcttg tttttcaaga aaactggcgc actgtacgca atgcctcttt gatggctttg
    80041 aaattccccc atgatgagta taggaaaggt cagcgtgaac tagcggtagc tgcttataaa
    80101 acgatccgta cgaaacagaa attgtttgta gaagcaccta ccggaacagg aaaaacaatc
    80161 tctacgttat tccccgcttt gaaagctgta ggggaagaag agggagaaaa gatcttttat
    80221 ctaacggcta aaacaataac tagacaagta gctgaagatg ctatgacagc attaaaagat
    80281 acaggagctg aagtaaaaag tgtcactttg acggcgaaag acaagatctg ctttttagat
    80341 gagacgacct gtaatccaga tcagtgccca tatgccaatg gttattacaa tcggattaat
    80401 gaaggtttat gggatctttt gaatcatgag aatcagatca ctcgtgaagt catcgaaact
    80461 tatgcaagaa agcatacact ttgtcctttc gaactatcat tagatgtcag tatctggtgt
    80521 gacgtaatca ttggggatta caattattta tttgatccaa ctgtttattt aagacgtttt
    80581 tttgaagatg aaaaaaacga ggactatctg tttttgattg acgaagcaca taatttggtg
    80641 aatcgttccc gagagatgta ttcggcagaa ctatcttatg aaaaaacgaa acgaagtaag
    80701 gaagctatac caaaggaatt caaaaaactt catcgccgtt tcaacaaact attaaaagaa
    80761 tttgattcga tccgagaaat agcaaaagaa gatcattggg attatcatca tcaaaaagca
    80821 ccggctgaat ctttagtaaa ggcaggttat caattaagcg aaaaaattaa agaatggctg
    80881 gcagaatttc ctgagcaccc atcacaagaa caattgcttc cttattattt tgatttgctt
    80941 cattttttga aggtcagtga attttatgat gatcactacg aaacaacagt cgaaaaaacg
    81001 tatcgcgatc tgatagtaaa agagttttgt attgatccaa gcttgtttct tgagcagtcg
    81061 ttggataaag gaaaaagcag tattcttttt tctgctagtt tttcaccact ctcttattat
    81121 caggaaacac tggggggaca aaaaagtctg gcttacaaac tgccgagtcc tttcccagaa
    81181 ggaaaccagc aagttttcat cgcaaattac ctagagacga cttatcgaaa aagggaacac
    81241 agtctggcaa aattagtaga gacgattcat gcttttgctg atggaaaggt cgggaattat
    81301 ttcgttttct ttccttctta tcaatattta gatcttgcgg tggaagcatt cagacagcaa
    81361 tatcccgaca gcaatgtgct aatccaagaa acggacatga atgaggaaga acgcgaacga
    81421 tttttaacaa aatttcaaac aaatcccgct gaaacattga ttggattttg cgtacttggc
    81481 gggatttttt cagaaggaat cgatttgaga ggggaccgat tgatcggcag tatgatcgta
    81541 ggtgtaggtt tgccgcagat gaatcatgag caggaactga tcaaatcata ttatgatgag
    81601 aaggaacacc ttggatttgc ttacgcttat cagcttcctg gaatgaacaa agtcttgcaa
    81661 gctggtggtc gagtgattcg tggcatggcg gatcaaggaa tcgttgtatt ggcagatcaa
    81721 cgattttcta gtttttctta tcgacgttta ttcccacaac attggcaaac agcaagagaa
    81781 gtacgaagtg ttggggaatt ggaagaagga atacagcgtt tttggttatc aaaagaagca
    81841 gtaaatcaaa aaaacgagga atgacagacg tcagcctcgt tttttgatta acgatagtta
    81901 gtgaattgca actcgattgg taagtcaagt ttacgtaata aagcaatcac agcttgtaga
    81961 tcatcacggt tcttacctgt cactctaatc tgatcttctt ggatttgtgc ttttactttc
    82021 agtttttcat ttttgatagc tgtagtgatt ttcttcgcgt tttcacgatc gattccgttg
    82081 accaaatcgg cagtttgtcg agctttgcca cctagagcgt gttctgattc ggaaaaatgg
    82141 atatttttga ttggcacatt tcttttgatc agcttaccaa ataatacatc tttgatctgt
    82201 tcgattttga aatcatcatc gctcaataca actagattgc tgttctctaa tttgatttct
    82261 actgtagaat ccttgaagtc aaagcgattg gacaattctt ttgttgtttg ctgaatcgca
    82321 ttttttactt cttctgtatt gacttctgat acaatgtcga agctagcatc ttttgccata
    82381 ggaaccctcc tgatatacat ataaatattt accatacatc tatcttatca taggattagg
    82441 gcagattctt acctgtttgc taaaattctt ctcatttgag ttcgaagtat tttattttaa
    82501 tagataaact gttattctta catttggaat accaaagtag gggagaggtg cttgtgagta
    82561 aaattatgac tgcgtatcat aaaaatgaaa gattaaagaa aatagtggtt attttgataa
    82621 ccggattgac cgctgcggtc gctttaaatt attttttgat tccagccaat gtcttttctg
    82681 cagggatgaa tgggatcgct caaatcattt cctcgctttt atatgaatgg tttcatatca
    82741 agatagacac aggaacattt atttttcttt tgaatatccc tgtgtttgta ttagggtttc
    82801 tcaaagtagg aaaacgagca acgatcctta gttttgtgaa tgttgtatgt gtatcggtga
    82861 tgacgatgct gcttccagtc ggacagatca ctgataatat tttgatgaat gccctagtag
    82921 gcggtgtttt attaggtctt ggcgtaggat tatctttaaa aatgggcttt acaactggtg
    82981 ggatggatat catctctctt gtactttcac aaacaactgg aaaaacagtc ggaaactaca
    83041 tgctgattct aaatggtatc attgtagtca tcgcaggctt cttctttaac tgggaaagtg
    83101 ccttgtatac gattatttcc atttatgcaa tgactcaagt aattgatgcc atccacacga
    83161 gtcatcaaaa agtaacagca atgatcgtta ccgtcaagcc agaagaggta acagaagaac
    83221 tagcaaaacg gatggtacgt ggaatgacat tactgccgtc tgtcggtggc tattcaaaaa
    83281 aacaagggaa catgatcatg atggtcatca cccgctacga aatgtatgat cttgatcaga
    83341 tcgttcatga aatcgacgac gatgcattta tcaatatttt accaactcat tcagtatttg
    83401 gccggtttgc aaatgaaaac gaacaaaaaa tgtatcgaac aactggcgta ttgccagaaa
    83461 taaaaaatcg ttcacaaaaa aaatgaggaa tcaggtttat tttagaataa atttaatcta
    83521 ttacatctaa taagaacatt tttataaatt taaacgagaa tcttacttct aaattctcat
    83581 ttacaagcag ctaaaaaaag agtatgataa ataaaaatcg tgttaaggag tgtcgtacga
    83641 tgagcgaagc aaaccaagaa cggtctggac aagaaataga actaatcaaa gaacgtattc
    83701 ttgctgcctt ggaaatggtg attgatcctg aattaggaat cgacatcgtc aatttaggct
    83761 tgatttatga tatagaattc aatcctgaaa atggagaaac agtcattaag atgactctaa
    83821 caactatggg ctgtccatta gcagatattt taacagaatc tatccatgac gcattaaaag
    83881 aagtgccaga agtaacgaaa gcagaagtca aactagtctg gtatccagca tggacgacag
    83941 ataagatgag ccgttatgca cggattgcgt tggggatacg atagaaatct tggctgacta
    84001 gctatgagaa gcaaatagga aaaacaaaaa agccttttat cctttctaga agaaaagaga
    84061 taaaaggttt ctttgtttat aaaattttgt ctgctatatt ataagttaat taa
//
LOCUS       DAPBCS010000005        72957 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPBCS010000005 DAPBCS010000000
VERSION     DAPBCS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 72957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.005"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(293..1918)
                     /gene="groL"
                     /locus_tag="RD521_000450"
     CDS             complement(293..1918)
                     /gene="groL"
                     /locus_tag="RD521_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDT7770213.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(1969..2253)
                     /gene="groES"
                     /locus_tag="RD521_000451"
     CDS             complement(1969..2253)
                     /gene="groES"
                     /locus_tag="RD521_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDT7770214.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            2478..3140
                     /locus_tag="RD521_000452"
     CDS             2478..3140
                     /locus_tag="RD521_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7770215.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(3216..4508)
                     /locus_tag="RD521_000453"
     CDS             complement(3216..4508)
                     /locus_tag="RD521_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7770216.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            4678..5307
                     /locus_tag="RD521_000454"
     CDS             4678..5307
                     /locus_tag="RD521_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDT7770217.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(5410..6219)
                     /locus_tag="RD521_000455"
     CDS             complement(5410..6219)
                     /locus_tag="RD521_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7770218.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(6274..7143)
                     /locus_tag="RD521_000456"
     CDS             complement(6274..7143)
                     /locus_tag="RD521_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDT7770219.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(7144..8460)
                     /locus_tag="RD521_000457"
     CDS             complement(7144..8460)
                     /locus_tag="RD521_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770220.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(8457..10292)
                     /gene="walK"
                     /locus_tag="RD521_000458"
     CDS             complement(8457..10292)
                     /gene="walK"
                     /locus_tag="RD521_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDT7770221.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(10297..11001)
                     /locus_tag="RD521_000459"
     CDS             complement(10297..11001)
                     /locus_tag="RD521_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7770222.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(11191..12051)
                     /locus_tag="RD521_000460"
     CDS             complement(11191..12051)
                     /locus_tag="RD521_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7770223.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(12038..12607)
                     /locus_tag="RD521_000461"
     CDS             complement(12038..12607)
                     /locus_tag="RD521_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDT7770224.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            12988..13060
                     /locus_tag="RD521_000462"
     tRNA            12988..13060
                     /locus_tag="RD521_000462"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13021..13023,aa:Lys,seq:ctt)
     gene            complement(13556..13942)
                     /locus_tag="RD521_000463"
     CDS             complement(13556..13942)
                     /locus_tag="RD521_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDT7770225.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(13939..14460)
                     /locus_tag="RD521_000464"
     CDS             complement(13939..14460)
                     /locus_tag="RD521_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7770226.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(14462..15496)
                     /gene="sppA"
                     /locus_tag="RD521_000465"
     CDS             complement(14462..15496)
                     /gene="sppA"
                     /locus_tag="RD521_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDT7770227.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(15529..16944)
                     /locus_tag="RD521_000466"
     CDS             complement(15529..16944)
                     /locus_tag="RD521_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDT7770228.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(16913..19066)
                     /locus_tag="RD521_000467"
     CDS             complement(16913..19066)
                     /locus_tag="RD521_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HDT7770229.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            19324..19560
                     /locus_tag="RD521_000468"
     CDS             19324..19560
                     /locus_tag="RD521_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HDT7770230.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            19579..19794
                     /locus_tag="RD521_000469"
     CDS             19579..19794
                     /locus_tag="RD521_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HDT7770231.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(20497..21249)
                     /locus_tag="RD521_000470"
     CDS             complement(20497..21249)
                     /locus_tag="RD521_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7770232.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(21264..22511)
                     /locus_tag="RD521_000471"
     CDS             complement(21264..22511)
                     /locus_tag="RD521_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7770233.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(22587..22733)
                     /locus_tag="RD521_000472"
     CDS             complement(22587..22733)
                     /locus_tag="RD521_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDT7770234.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(22837..23148)
                     /locus_tag="RD521_000473"
     CDS             complement(22837..23148)
                     /locus_tag="RD521_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7770235.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(23150..23347)
                     /locus_tag="RD521_000474"
     CDS             complement(23150..23347)
                     /locus_tag="RD521_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDT7770236.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(23741..25468)
                     /locus_tag="RD521_000475"
     CDS             complement(23741..25468)
                     /locus_tag="RD521_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDT7770237.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(25461..26654)
                     /locus_tag="RD521_000476"
     CDS             complement(25461..26654)
                     /locus_tag="RD521_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7770238.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(26841..27485)
                     /locus_tag="RD521_000477"
     CDS             complement(26841..27485)
                     /locus_tag="RD521_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7770239.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(27579..27713)
                     /locus_tag="RD521_000478"
     CDS             complement(27579..27713)
                     /locus_tag="RD521_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDT7770240.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(27715..29847)
                     /gene="feoB"
                     /locus_tag="RD521_000479"
     CDS             complement(27715..29847)
                     /gene="feoB"
                     /locus_tag="RD521_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDT7770241.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(29844..30317)
                     /locus_tag="RD521_000480"
     CDS             complement(29844..30317)
                     /locus_tag="RD521_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDT7770242.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            30718..30942
                     /gene="nrdH"
                     /locus_tag="RD521_000481"
     CDS             30718..30942
                     /gene="nrdH"
                     /locus_tag="RD521_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDT7770243.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            31101..33260
                     /gene="nrdE"
                     /locus_tag="RD521_000482"
     CDS             31101..33260
                     /gene="nrdE"
                     /locus_tag="RD521_000482"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDT7770244.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            33310..34275
                     /gene="nrdF"
                     /locus_tag="RD521_000483"
     CDS             33310..34275
                     /gene="nrdF"
                     /locus_tag="RD521_000483"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDT7770245.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(34364..35503)
                     /locus_tag="RD521_000484"
     CDS             complement(34364..35503)
                     /locus_tag="RD521_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7770246.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(35812..36525)
                     /locus_tag="RD521_000485"
     CDS             complement(35812..36525)
                     /locus_tag="RD521_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDT7770247.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(36779..37480)
                     /gene="nagB"
                     /locus_tag="RD521_000486"
     CDS             complement(36779..37480)
                     /gene="nagB"
                     /locus_tag="RD521_000486"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDT7770248.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            37714..39246
                     /locus_tag="RD521_000487"
     CDS             37714..39246
                     /locus_tag="RD521_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7770249.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(39437..40639)
                     /locus_tag="RD521_000488"
     CDS             complement(39437..40639)
                     /locus_tag="RD521_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7770250.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(40721..41410)
                     /locus_tag="RD521_000489"
     CDS             complement(40721..41410)
                     /locus_tag="RD521_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7770251.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(41516..41884)
                     /gene="rplL"
                     /locus_tag="RD521_000490"
     CDS             complement(41516..41884)
                     /gene="rplL"
                     /locus_tag="RD521_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDT7770252.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(41937..42440)
                     /locus_tag="RD521_000491"
     CDS             complement(41937..42440)
                     /locus_tag="RD521_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDT7770253.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(42644..42771)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(42850..43539)
                     /gene="rplA"
                     /locus_tag="RD521_000492"
     CDS             complement(42850..43539)
                     /gene="rplA"
                     /locus_tag="RD521_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDT7770254.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(43643..44065)
                     /gene="rplK"
                     /locus_tag="RD521_000493"
     CDS             complement(43643..44065)
                     /gene="rplK"
                     /locus_tag="RD521_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDT7770255.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            44408..45076
                     /gene="sdaAB"
                     /locus_tag="RD521_000494"
     CDS             44408..45076
                     /gene="sdaAB"
                     /locus_tag="RD521_000494"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDT7770256.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            45089..45961
                     /gene="sdaAA"
                     /locus_tag="RD521_000495"
     CDS             45089..45961
                     /gene="sdaAA"
                     /locus_tag="RD521_000495"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDT7770257.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(46052..47071)
                     /locus_tag="RD521_000496"
     CDS             complement(46052..47071)
                     /locus_tag="RD521_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDT7770258.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(47244..48149)
                     /locus_tag="RD521_000497"
     CDS             complement(47244..48149)
                     /locus_tag="RD521_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7770259.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(48208..48753)
                     /gene="nusG"
                     /locus_tag="RD521_000498"
     CDS             complement(48208..48753)
                     /gene="nusG"
                     /locus_tag="RD521_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDT7770260.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(48854..49024)
                     /gene="secE"
                     /locus_tag="RD521_000499"
     CDS             complement(48854..49024)
                     /gene="secE"
                     /locus_tag="RD521_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDT7770261.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(49048..49200)
                     /gene="rpmG"
                     /locus_tag="RD521_000500"
     CDS             complement(49048..49200)
                     /gene="rpmG"
                     /locus_tag="RD521_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7770262.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            49362..50006
                     /locus_tag="RD521_000501"
     CDS             49362..50006
                     /locus_tag="RD521_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7770263.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(50062..50565)
                     /locus_tag="RD521_000502"
     CDS             complement(50062..50565)
                     /locus_tag="RD521_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDT7770264.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(50578..50624)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            51162..52127
                     /locus_tag="RD521_000503"
     CDS             51162..52127
                     /locus_tag="RD521_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDT7770265.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(52226..53398)
                     /locus_tag="RD521_000504"
     CDS             complement(52226..53398)
                     /locus_tag="RD521_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770266.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(53385..54530)
                     /locus_tag="RD521_000505"
     CDS             complement(53385..54530)
                     /locus_tag="RD521_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770267.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(54544..55468)
                     /locus_tag="RD521_000506"
                     /pseudo
     CDS             complement(54544..55468)
                     /locus_tag="RD521_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            55585..56664
                     /locus_tag="RD521_000507"
     CDS             55585..56664
                     /locus_tag="RD521_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7770268.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            56696..57289
                     /locus_tag="RD521_000508"
     CDS             56696..57289
                     /locus_tag="RD521_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7770269.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(57399..57626)
                     /locus_tag="RD521_000509"
     CDS             complement(57399..57626)
                     /locus_tag="RD521_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7770270.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(57729..60317)
                     /locus_tag="RD521_000510"
     CDS             complement(57729..60317)
                     /locus_tag="RD521_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDT7770271.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(60337..60996)
                     /locus_tag="RD521_000511"
     CDS             complement(60337..60996)
                     /locus_tag="RD521_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7770272.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(61045..61548)
                     /gene="trmL"
                     /locus_tag="RD521_000512"
     CDS             complement(61045..61548)
                     /gene="trmL"
                     /locus_tag="RD521_000512"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDT7770273.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(61769..62635)
                     /locus_tag="RD521_000513"
     CDS             complement(61769..62635)
                     /locus_tag="RD521_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7770274.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            62744..63385
                     /locus_tag="RD521_000514"
     CDS             62744..63385
                     /locus_tag="RD521_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDT7770275.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(63447..64808)
                     /gene="mgtE"
                     /locus_tag="RD521_000515"
     CDS             complement(63447..64808)
                     /gene="mgtE"
                     /locus_tag="RD521_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDT7770276.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(64826..65725)
                     /locus_tag="RD521_000516"
     CDS             complement(64826..65725)
                     /locus_tag="RD521_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7770277.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(65727..66524)
                     /locus_tag="RD521_000517"
     CDS             complement(65727..66524)
                     /locus_tag="RD521_000517"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDT7770278.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(66502..67206)
                     /locus_tag="RD521_000518"
     CDS             complement(66502..67206)
                     /locus_tag="RD521_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDT7770279.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            67337..67912
                     /locus_tag="RD521_000519"
     CDS             67337..67912
                     /locus_tag="RD521_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDT7770280.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            68030..68707
                     /locus_tag="RD521_000520"
     CDS             68030..68707
                     /locus_tag="RD521_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDT7770281.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(68786..70594)
                     /gene="pepF"
                     /locus_tag="RD521_000521"
     CDS             complement(68786..70594)
                     /gene="pepF"
                     /locus_tag="RD521_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDT7770282.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(70770..71768)
                     /locus_tag="RD521_000522"
     CDS             complement(70770..71768)
                     /locus_tag="RD521_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDT7770283.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(71994..72647)
                     /locus_tag="RD521_000523"
     CDS             complement(71994..72647)
                     /locus_tag="RD521_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDT7770284.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        21872 a        15043 c        12397 g        23645 t
ORIGIN      
        1 ttacttttaa tagataaaat attataacta ttaaaaataa ctattttcat tataattaca
       61 cacaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc taaacacgta
      121 taaacagcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa
      181 aagcaatttc cgtgaatttc ttcttatttc ttggagttaa acgcttctgt tccagcctct
      241 ttttatctgt ctttcactaa tgatcagttg tagataagca agttgctttt tcttacatca
      301 taccgcccat cattgatgga tccattgcag gagctgctgg cgctgctggt tctggtttat
      361 ctgcaacgac tgcttctgtc gttaataaca aggcagaaac ggaagcggca ttttgcaagg
      421 cagaacgtgt tactttagtt gggtctacga tacctgcttc aaccatgttt acccattctc
      481 cagtagcagc attgaaacca gtgcctagtt ccacgttctt caatttatca acaatcactg
      541 atccttcata accagcgttt tcagcgattt gacgaattgg ttcttctaaa gcacgaacaa
      601 cgatcttgat acctgttgcc acgtctcctt ctgcttctac agcagaaact ttgctgataa
      661 cgttcactag agctgtacca ccacctgaga ccattccttc ttctactgcg gcacgtgtag
      721 cattcaatgc atcttcaatg cgtaatttta attctttcaa ttctgtttca gtagctgcac
      781 caactttaac aacagctaca ccacctgcta atttagctaa gcgttcttgt aatttttcgc
      841 ggtcaaaatc agaagttgtt tcagcaattt ggtttttgat caattgcaca cgagcttcga
      901 ttgcttcttt ttctccagaa ccttctacga tcgttgtatt gtctttatct acaacaactt
      961 tagaagcatt tcctaagttt tcgattgttg catctttcaa ttctaaacct agatcgtcag
     1021 taattactgt accacctgtc aaaatagcga tatcttcaag cattgcttta cgacgatcgc
     1081 cgaaaccagg agcttttaca gctactacgt tgaatgttcc acggattttg ttcaatacaa
     1141 gagttggcaa tgcttcacca tctacatcgt cagcaataat caacaatgga cgtgattgtt
     1201 gcaagatttg ttctaacaat ggcagaatat cttggatgtt agagattttt ttgtctgtga
     1261 tcaagatata tgggttttct agaacagctt ccattttgtc gttatctgtt accatatatt
     1321 gtgataagta accacgatca aactgcatac cttctacaac atctagttct gtttcgatcc
     1381 cttttgattc ttcgattgtg atgacaccgt cgtttccaac tttttccatt gcatcagcaa
     1441 tcaaatgtcc aactttatca gaacccgaag aaacagctgc tacttgtgca atggcttctt
     1501 ttgaatcaac aacagttgag atattatgca attcttcgac tgctgctttt gttgccaatt
     1561 cgatcccgcg acgaatgcct agaggattag cacctgcagt tacgtttttt aaaccttcgc
     1621 gaacgattgc ttgtgtcaga acagttgctg tcgttgtacc gtctcctgca atatcatttg
     1681 tttttgatgc tacttctgat acaagttttg cacccatgtt ttcaaaatga tcttctaatt
     1741 cgatttcttt tgcgatcgtc acaccatcat ttgtgatcaa tggagagccg taagattttt
     1801 ctagtacgac atttcggcct ttaggtccta atgttacttt cactgtatct gctaatttat
     1861 ctaccccgcg taacatagct gcgcgtgcgt cttctgcaaa tttcaattct tttgccatga
     1921 ttgattcacc tcattgatta tttttgatta tttttttatc ttccaaattt actcaacaat
     1981 cgccatgata tcttttcctg cgacgatcag atattctttt ccttcgtatt ttacttctgt
     2041 accggcgtat ttttcgaaca taacttggtc gcctgctttt acagcggcag gaactttttc
     2101 accgttttct aacaaacggc cttcaccgac tgcaacaact gtcccagttt ggggtttctc
     2161 tttagcagca gaagctaaaa cgattcctcc gacagttttt tcttcttctt ttgcgacttc
     2221 gataatgacg cgatcaccta atggttttaa cacgataaat ccctccgata actctttttt
     2281 taatctgtta gcactcttaa ccttcgagtg ctaacctaca tttttaataa tactcattct
     2341 ccctcttttt tgcaagcttt taaatcaaag aaaagaaatt ttattctgac agctgcttat
     2401 tgaaattatg atataattga aatcaatcga tataagcttg ccttgcatgc ttgttatccc
     2461 ttaaaaggag gaattacatg tctttaaaaa aatatagctt cctcagcatt ttttgctatg
     2521 gtctaatttt tatctcaccc ttgatcctag cttcgatagg acttgtaaaa tctacgtctg
     2581 aattgatcac agcaactgca gtcatgtata tactaggcgc cgttgtccta gctatttttt
     2641 attttaagca acgtgaaccc ttagcgatcg aatcagcggt aaaaaaagct tctgctccaa
     2701 agattgttat ttatggattg gttggaatct ttgtcgcatt gattctgcaa agcatcgctg
     2761 tcatgttaga atctgtattg tttggcaaag caaccccttc cgagaataca caaaatatca
     2821 tccagatgat catggaagca cccgctttta ttttggcgac aactgttgct ggacccatca
     2881 tggaagagtt cgtattccgc cgcagtatac tggggatcat cagtcgatat tcaaattttt
     2941 gggtcggagc agtgatcagc tctttgctgt ttgcgtttgc tcataatgat ggtcatctgc
     3001 taatctactt tttcctaggt ttcttcttca gcctagagta caaagcaact ggtcggatct
     3061 ggacatcgat gatcacacac gtaggaatga acacattagt tgttcttgtt caattagctg
     3121 tccaaaaagg gctagtataa acatgactat aaatactcct gtcatcctag attggtgatt
     3181 ttcccaatct aaagcgacag gagtattttt gttttttagt cgttcatctt ttctgctgct
     3241 ccatcatttt ttgtctgtct ttcctttttg cagtttgcag aatctatttt tttcaaaaat
     3301 gggaaataaa tgaaaaaact aattgacaac acgatacctt gtaaaatcgc tgcaggcact
     3361 ccgcctacta agaaacccga gaggatcggc ggtgttgtcc aagggacttg gactgctgta
     3421 aataaaggaa cgaatccaaa atataaagcg gaataggtaa tcaatcctga aacgaccggt
     3481 gcgaaaataa aaggaacagc catcatcggg ttcatcacga tcggtgtgga aaaaataatt
     3541 gattcattga tgttgaaaaa ggctggtaaa agagcaagat ttcctaattc tctgtatttc
     3601 ttcgatttcg caaaaaccgt cataaaaaat acagccccta acgtcaatcc agcacccgta
     3661 accgtcatga actgatcgag aaattgctga gtaacgatat gtccgccatt tgccaccgtc
     3721 aactcttgtc ctgaggctaa gatttctgca tttgctaatg aattcgcttg caagacggga
     3781 ctaaccaacc cattgaccat cgcagatcca tgaagaccaa ataaccataa aaacggaaca
     3841 aaaaaaccta ataaaagcac accaaacaac gtgtctatga acgattgaat gggaagctgg
     3901 acatatgtcc atactccttg aacaacagtc attctgaaca aatgttgaaa aaccgctgaa
     3961 ccgataagcg taaaagtgac tataacaatg attggaatca atgaagtaaa agactgccaa
     4021 acattttcag gtatcttctc tctttgcttc caccctttcg caagtcttac taaacgtgca
     4081 tagccccatc cgatgatgag cccagttgta actgctccta gtatcccttc agcacctgtc
     4141 cagacagtat ctatctgagt atttagtggc tgaataagca gtagataagc agacaaggct
     4201 gttatgccga ctggtaagcg atcgatgtct tcttcttttg cataagcgta tgcaatggca
     4261 aaaacgatcc aaaaggctat cacatcaaat gtagcctggt atacttgtgt aaaatacata
     4321 gaaaatcctg ttttgtctat ccactgagcg atgtaatcca caggaaaatg agcaaataag
     4381 aaaaacagcg agccaacaat aattattggg atcgtgtaag tcattccatt acgcaaagca
     4441 gttaagggtc taagattcac aacagatgtt atcattggta aaaaggcacg atctaaccac
     4501 tctttcattt cttatctcct tcaacttcta tttttatcaa tcagttttct taatttaact
     4561 atacccaatg atcacgaacc atgaaaggaa tgcgcactga gcttttcaaa aatcggattc
     4621 tttgacgaat attcagcaac tgcttacaat ggacaggaat aaaaaaggag gttttttatg
     4681 aaaattgcac tcattggagc tactggccat gcaggttcat ttattttaga cgaagcattg
     4741 tccagagatt tagacgtgac ggcaatcgtg cgtcatccag aaaaattgcc atcagatgtt
     4801 ccgtttatac aaaaggattt atttgagttg acaactactg atcttgcatc ttttgatgtt
     4861 gtcgttgatg ctttcaacgc tcccaaagga aaagaagaaa tgcatcaaac ttctttgtcc
     4921 catttgattg agatgcttga aggaaccgat acacgcttaa ttgtcgttgg gggtgcctct
     4981 tcgttattct tagatacaga aaagacgact cgtatgattg accaagtacc taaaaatgca
     5041 cccttctatc caacagcttt caatatgtac gaaggattgt taaaattgaa acaatctaaa
     5101 aatctacatt ggacttatat cagtccagct tctttatttg atccacatgg caaacgtaca
     5161 ggtacttatt tgttaagtga tgattattta caaagaaacg ctgctagcga cagtacgatc
     5221 agcatggcag attacgcgat tgcattagta gatgagatac tggatgagaa acatgaaaag
     5281 cagcacatca gtgtagtcag tcgataatac ggttattttt ttaatatgct aaaaaaataa
     5341 aaatagaaga taaaaccaac cctgattgat tttatcttct gctttttata tttttattga
     5401 ttgaactgtt taaatcgaaa atagctcagt tgcttcatct ggatctgtat catagatttc
     5461 aaaatcatgt ccaacgccaa aatctttttc tttcaaaaca ttttccattg ttaaatgagc
     5521 aagttctttc atattgtttt ccttgctaag atggccaaga tagattcgtt tggtccgatt
     5581 accgatcaca tcagtcatga caagtgcacc gtcttcgttg gaaagatgtc ctctatctcc
     5641 taaaatacgc tgttttaaat tccacggata aggtcccatg cgtaacatct caagatcatg
     5701 attactttcc atcaaataac catcggcatt ttcaatgaca cctcgcatat aatcactgca
     5761 atatccggtg tccgtcaaca tgacgaatga ccgattttct ttatggaaac gataaaattg
     5821 cggagccgct gcgtcatggg agacgccgaa actctcaata tcgatatcgc cgaatgttct
     5881 gactttcccc atctcaaata gatgtttttg ttccactggt acatttccaa tcagtggttc
     5941 catcgcttcc caagtctgct gattggcaaa aacatccaaa tgatacttac gagccaagac
     6001 acccacgcca tgtatatgat ctcgatgttc atgcgtcaca agtattccat ctaattggtc
     6061 cggtgtccga tcaatttgag ccatcaagga agtgattttt ttaccgctaa gtcctgcatc
     6121 gaccaaaagc cgtttttttt cactttcgat ataaagtgaa ttacccgaac ttccacttgc
     6181 taggatactg attttaaaag ctggtttttc ttcagccatt tgactcctac ttcccttctt
     6241 actttgtaaa cttttttcat tatactcgct tttttattga tcttccactt ttggtacagt
     6301 attgtttgtc acgatcgtat tgctgaaagc gttgacgctc tcaatttgga ccactttatc
     6361 gcttgtttcg atcgctacat gccaaacagg aacatacaca tttttttccc ggactttcag
     6421 aatacaggaa taagctagct gacgccataa aatttttgat cctcttggta ttttgttatt
     6481 gatgtacaat gtattgattg cttcctcttc cgtatacagt tccgtctttt cgcgcagctg
     6541 ctcgatatca gataaatgcg tctgcatgta tttcgtgatg ctataatcat tatcgttttt
     6601 ttctaaagaa agaacgattt ttgctgtatc atcattgaat gggatgcctt catactcttg
     6661 gcttgcttgg atttctagtg gttcttcttc taaatgagaa aagtttttcg tgtacacata
     6721 atcattaccg aataaaatat tgtctttgtt agataaaaaa tcttccaagg aactactaat
     6781 tttatctgga tcgatcacgt tcttttcagc atctgaactg gataaagatt tcgtcaaaac
     6841 atcaccgtct aaaaacacac cacttgccag caagccgctt ttaccagcct tgcgataatt
     6901 cgctaatgaa gtgctaagat tggtctgctc cgcgctaaga taatagcctt gcttgttctc
     6961 agacgaaatc ttccccgtgt aagtgatatc gtcgctagcc agacgttgtt ggattgattc
     7021 agtctgatta gagcgaatga cattgttttc ttggtatacc gcgtcatgat aactgctgaa
     7081 taaaaacaca ttcaacccta aaaaagcgac gaaaaatatc cattcgattt ttttgaaatc
     7141 caattaattc gcctccaatc caggtaattt ttccagaagg tcgttagctg aataccagtt
     7201 atctttatat ttcacatacc attcaggtaa gagatcaacg acacgattcg tttctttgac
     7261 attttgccat gtatagccaa cgatcactga accaatcttg ttgctatctg ctcccttagc
     7321 ttttaactgc tggatgattt cattcgtact tgcaagttct acttcttcgt ctgaaggtat
     7381 cggcacttga atcgtattca aactggtttg gatattcact tgcacagaac cattcgtttc
     7441 accgccaact tccacaccaa tctgtcctct gctgtctgtc ccaaaaaccg ggaagccttc
     7501 cacgaatatc cgataatcga tcgtattgtc actgcggtca aaatatctga ggttccctaa
     7561 agaactgcct aatttgctaa tataggggaa actttcagaa aatacactag cattttcttt
     7621 tttctctggc agctctccac gaaaatcaac tacttgcgtt tccgaatgga tagacattgt
     7681 ttcattccca ccgtaaaaaa tcaattcatt gctttcatca ttatttttca cgctatctgg
     7741 tttctggaaa aaagcatttc taaaaagtgt atagggttgc tgtgacagga tataactata
     7801 cttcttcagt ttgatcgaat gtttcgtagc atattgcgtg tcgatcgtcg atggttctgt
     7861 tgtcatttct gtccattttg tgtcttttgc tgttaattct ttattgatat ctgtccaatt
     7921 caacgaaata tttgcttcat atacgagatg gttcgaataa ttcaaaaatc ggattttttt
     7981 atttccttga tcgaattgta ttctagtaaa atacatattt tcgctttctt ttgccaagtt
     8041 cgacacatct aaatctaaat caaatgcttc cttgtattct cttagcaaaa aaggcgcatt
     8101 ataggccaac tcaaaaccag agttcagatt tttcacctgt tcaaactgtg atttgtctcc
     8161 tgaaactact tcagaaagct ttccaaatct tgctttatcg atgatcaatt gcaatcggta
     8221 cacgagattt tcactgttgg tttcttggat cattcccgaa tggtgccatg taacatgaag
     8281 cggaagaaag acttcggaag tttttcgatt gctttgagac tcgatcattt gagtgtttgt
     8341 ctcatctacg ttgattgaac tttttcctgc tgggctcagc cagattgcat aagagaagta
     8401 tatgcttaac gcaaccatca aaatgagtcc tatacgaacg actttttcag agattctcat
     8461 tcccaccatt cctcctcata aggttcatat ggcagcgaga tataaaaagt cgaccctttt
     8521 ccttccttgc tctctgccca aatagcgcct cgatgcgcct taatcacttc tctagaaata
     8581 gctaatccaa gtcctgtccc accttgtgct cttgcacgtg ctttatctac tcgatagaac
     8641 cgctcgaaca ctcggttcag atctttttta ggaatcccca atccttgatc ggtgatactt
     8701 aaaatcacgt tattatgcgt ttctaataag cgacaagtaa tcgttccccc atctggtgaa
     8761 tacttgatcg cgttattcat gatattatcg actacttgga tgattttatc tgtatctact
     8821 tcgacccaaa gatctctttg cgtaaattca cgacggatct tataattttt ttcctgattt
     8881 cccaccatca tatcaaaccg gtcaagaaca aaattcacta actcgttgaa attgacatat
     8941 tctaattgaa gctgggtatt acctgtatcc atgcgagata gatttaagag gtcattgatc
     9001 attcgaatca ttcggtctgt ttcatctagc gttaccttca aaaaatttgg tgctacttcc
     9061 tcgtctttcc acgcaccttc gctcaatgct tcgatataac tacgcatact tgtcaacggt
     9121 gtacgaagtt catgggaaac attagagacg aattcacgac gttcccgttc tgttttttct
     9181 tgttctgtca catcatgcaa gacagctact aatccgctga taaaacctga ttcgcgacgg
     9241 atcatagaaa agtctactcg caagatcatc tgatcacttt ccaatgtact ggttgaacgg
     9301 tcaagtacca tttcgtttgg atcttctaat aatttccgca acgtatattc ttcttcgata
     9361 tctaaaagtg tcaaaatcga ttgtccgatt gcttcctcgt ttttgatatc tagcaaggaa
     9421 acagccatat cattgatcgt aatgacttta cccctgcgat ctgtcgcaat gacgccgtct
     9481 gtcatatggg atagcacgct atccaaacga ttgcgttcag cttccattgt atcttgagct
     9541 tcttcaatac gttcagctaa ctgattgaag gtatcagcca gctgtccaag ttcatcttgt
     9601 ccatgcactt tgactttccg actgtagtct cctctagcga ttcgaatggc ttgttctctc
     9661 atttcaccga tcggctgtgt aatcgaacga gcaactaaaa cagatacgat cattgaaata
     9721 aatgccgcaa ttagtgaggc agtaaagaaa atgactgctg tgttattgat ttccgaatat
     9781 ttttgttcga tattactttt gacgtaaaga gccccgataa cggcatcgcc ggtcggggac
     9841 aggatcggtt gaacattgat attcacccgt ttatcgtcgt tatctaaagc aacataacgt
     9901 ttagtcgtga aatcattgat atcaacataa tcatttttct ttcccacagc gctttgttca
     9961 ttgacatcgg ttgttcctcg gatgatccct ttttcgtcaa cgacccgtat ttcgatgata
    10021 tcgctagtcg cactgttttc aataaatctt ttgatattcg catttacctc ttcatcatct
    10081 gcattatctt ttcgattcaa ttccgggctg attgttgttg ctaatgattc caccgtttga
    10141 ttcatatctt taataaatgt attgatcgtg gaacgttcta aaccacgtat aaagtaggca
    10201 ccaataattt cgatcgagat caataaaatc agaataaagg taatagcgat cttaaaattg
    10261 actgatcgaa aaaagcggac ttttcttttc atccaattac tcctgttctg ggttacgcaa
    10321 atagtaaccg acaccacgac gtgtcactaa ataatttgga tggcttggat tatcttcgat
    10381 cttttcacgc aaacgacgta cagttacatc cactgttcgt acatctccaa aataatcata
    10441 accccaaacc gtttgtaata aatgttctct tgtcatcact tgtccaaggt gcttcgccaa
    10501 gtaaaagagt agttcgaatt cacgatgtgt taactcgatt gtttctcctc gtttcgtcac
    10561 catgtaagct tctggatgga tcgtcaaatc accaatcgtc aattccgcag gtgctgcctc
    10621 ttctggttcc ttcgcagccg tggcaccgcg acgaagattt gctttgacac gtgcaactaa
    10681 ttctcggttg gaaaatggct tggtcacgta atcatcggcg cctaactcta agcctaggac
    10741 tttatcaatt tcagaatctt tggctgttac cataatgatc ggcatatcat aagttttgcg
    10801 tacctcacga gcaacttcta agccgtccat tttaggtagc atcaaatcaa gtaaaatcaa
    10861 atcaggttct acctcagcaa ctttttccaa ggcttcttcc ccatcatatg cagtataaac
    10921 gtcatagcct tctttggcta aattgaattt gacgatatcc gaaatcggct tctcatcatc
    10981 gacaactaaa acttttttca taaagagcca cctcttatat attattgtca taccttctca
    11041 ttataccgta tttctgcttt tgtttcatct ttgcctataa aacaaaagag aaaattaaaa
    11101 tgaaagtgca aaatacttta ttattttttg ttctaacttc aataaaaaag gaaaacactg
    11161 attttcacct cagtgtttcc tttttttgat ttatccttct aaaacttgta caccgacacc
    11221 caataatccc ggaccagtgt ggactactaa tgccggagaa atctggccaa agtagatttg
    11281 gtttgcttgt gggaactctc ttttaagtct cgcttccatt tcaattgctt cttctttcgc
    11341 atccccatgg gcaacagcta agttgaagac ctttgcatta ccgatttttt ctttgacgta
    11401 agcaaccgtt ttatccaaac tttttttacg tcccctagct tttcctactg tgtaataaat
    11461 cccttcttca ttacatgaga tgattgggtt aagctttaaa gcggttccta caatagaagc
    11521 aactaaaccg atccgtccac ctttttgaag atattccaat gtcgcaacat taaagaaaac
    11581 ttttgttctt tcgactaact cattcagatt actgatcagt tgatcccatt ctactccttc
    11641 ttccagccat tttgctgctt ggatcgcaga aaaacctgca ccaataccaa tatttttcgt
    11701 atctagtaca tatgcttcca attcttcttg ctgttgtgca attaaacgaa ccacattata
    11761 tgtcccgctc aatccactgg aaattgttac cgcaagtacc ttttcataac cgtctttttt
    11821 gatttgctca aagatttctg tgatcgactc accatccggc aaagatgtac cagggatttc
    11881 ctctggcaaa cgttgataga tctcttcagg ggtaatgtca attttatctg tatatacctg
    11941 atctttataa ataattttca atgggatcat atacatctcg tattttttta tcaattcttc
    12001 tggaacatcc gtacctgaat ctactaaaat tgcgatttta ttgtttttca tttgttttct
    12061 cctcttttaa atattcggtt tctagaacaa tcgttttttc cgcaagaagt ttcattgcaa
    12121 acgataaagt tgccgctctt acagcaatat attctacagg ggttgcctgg tcgaattttt
    12181 gcggatgaga tttaccttgt gctaattggc tgaccatcca cacagctgct tcttgttcat
    12241 cgcaaaatag attataggct tcacgaattc ctactgtttt tccttggaac aatatccctt
    12301 ctttgatttc aggaatagtc aatacttgtt tcaacgtagt gattgcaagt aaaaatgcta
    12361 cgtgttcttt gttatatctt tttttaactg gtgctggtat caaacctagt ttaacgtaat
    12421 tgtttaccat agaagaggta agcaatggat gcttttctcc ttgtatcaca ggtgaaagat
    12481 aacgatcaac taacgttctg acttggtcca tataaagttc taagtcaggg agttcttccc
    12541 agtgaggcag acggaatgtt tctagttcct tcccccactc cattaattgc tgacgcattt
    12601 tttccatcat aaccgctcct tttttcattt cataaacatg atatcagact tatgataaac
    12661 attcaatcta gttgctttaa ctaggtgatt tgattagcgg gattctatga aaacaaaaaa
    12721 caagaaggct aagccttctt gtcttttatc gttaagaaca ggagacccgc cggggagacg
    12781 gcgggaaagg agtaaaaaat gaaaaaagtg ttagggtttg ttctcttgca agaactttgt
    12841 ttgttggtat gcttatataa tagcgagcaa atgtgaataa tctatgaatg atttcctata
    12901 aggaagtaaa gatttttctg attttcttta aatgtagtct tgcattttaa aattcttagt
    12961 gctactatta ttaagtactt tttgtacggg ccgttagctc agttggtaga gcagctgact
    13021 cttaatcagc gggtcgcggg ttcgagcccc tcacggccca ttgggtgcca aacccacgga
    13081 acaggtaaat gttggtgcga aagcatttcg aaaacattta cgctgttctt tttttatttt
    13141 tcaaaataac tagggctttt tatttttaac tcaaagtcca ctgtagccct ttggactttg
    13201 agttaaaaaa gaagcataat tatgaacagt aaagtttcta atataacaat aattcgagaa
    13261 gctttgcaaa atcatgctgc caatgaactc tctaaacata taggattaaa tctaagtact
    13321 ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact aaatttactt gctgctttcc
    13381 ccttttccat taatacaaca atcagagagt gtcttttttt cgaaaaaatt agaataaata
    13441 aaagacacgt agttttttta tctacatgtc ttccatcgac tacaagttat catttctcaa
    13501 aacgtattta gcttttaatt gattaatcgt ttcacgttgt ctacacattg cctaattaag
    13561 attttcttat tagaagcatc aatcccgcaa taatacctaa aactccagga attacccaca
    13621 ataatgttaa aagaaaaaat ggaataatag ataaaatgag taaactaata ccatagacca
    13681 ctgttttttg attttttctt actaaaaaac ctattatgaa aatcattatc gaaaaaacta
    13741 gggtaattcc tccaaataaa acaacattcc cgcttagatt gactaacata tctatatcct
    13801 tactggttag ttctgtagta tttaaagtag ttgaatcaat agaagataga aacataccac
    13861 ccatcaatgc taaaagacta cataaaatcc ctgttattcc actaattaaa atcaaaataa
    13921 actctgtttt tctcttattc atttaattct ctccagatta agtgttttag aattagaaat
    13981 tttttgacca tagttaatag cttctaatga acgaataaca accactgacg tgttagaaaa
    14041 ataatcccca atatgttgtt tcttgggcgt gaaagcagcc accaaatacc caatttgaaa
    14101 tacaaaattt tttaatataa acctacatat acactcacga ataaagatag tttgccaact
    14161 tagctctttt tcctcaaagc tcactactct tatcccaaaa atcattttcc caattgtttg
    14221 tcctgaattc agcttagtca aaagtacaaa ataggttaaa taaatagcta atgacgctat
    14281 accataaaaa gtaataatgg atttgtcaga ttccagtccc atgattttgt aaaagagacc
    14341 tatagtagca tgggtgatga tgctaataca aattaaatca ataagaaaag caaaaaatcg
    14401 taaccaaaat cccgcaaata cataattagg caaagtttca atgccactct tcttattcat
    14461 tttatagccc tccatagtaa tacattggtt ttggagtatc agaaaatcca agtttttcaa
    14521 aaactgaaat tacacgatct gatgtagttg cctttagccc ttgccattca gcaatcttat
    14581 tacctaacca agaagaagca aagccagttg tgtcattttt atattctata accgtcgcat
    14641 cagttaattt cttctctttt tttagggaat ctaatgcatc ttcagagtaa ccaatttgat
    14701 ccaccaaccc attttctact gcttggttac catcataaat tcttccatcc gcaagttctt
    14761 tcactgcatt agtatccata tttcttcctt ttgctacaac attgacaaaa cgatcataag
    14821 cactcatcac aaattcctgt ataactttat tctcttcctc tgaaggtttg tgttgaggtg
    14881 acatcatatc ttttaatgca ccacttttat acgtcgcatc agttacgcca agtttttcta
    14941 ataagccaga ataatttaaa ccagaaataa tgacaccaat tgatccagtt gttgtttctt
    15001 ccgtagcaaa tattttatct gaaccagcag aaatgtagta ccctccactt gccgctgtat
    15061 ttttaaatgc agtatagata ggaatatcta gctttttaat ttttgccatt tctttagcaa
    15121 tctcagcact ttcatatatt cctcccccag gtgaatttac ttctaataac actcccttaa
    15181 ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa attctgatgg ttatattgtt
    15241 ccctactaaa aagattgctt tcacctgtat ctgcaataac tccattgacg gatagtttga
    15301 caatcttttt atttgatgcg ccttcttcta acgtttcttc tgtgatttcg ttagatccgt
    15361 aaaaaatctt atttattcct gttagggttg tatttgatgc ttcctcttta tcttttcctg
    15421 gaattagcgc gacgataatg gatagtaaca ataatccgca cgctactccc acagcaatcc
    15481 atcttctttt attcatataa actcctccta tattcttagt aaaaataatt attttttatt
    15541 tagaaacaca tcgacaattt gttgccaata tgttttttct cctacaatta aagacaactc
    15601 tccagttaat ccataacgaa acggaaacga ttgagttgtt tttaacgttc ctttcactgc
    15661 ataaaaaaca cctttctcag tggcagtaga attttctgaa atttcatcta aagttccgtc
    15721 gatcgtaatc ggagccccac ctttttgatc caatttaaaa tgaaccttca tcccttcttt
    15781 aatataggta ctttcattcg caggtaaaag tgctgtaaat ttcatttttg atttttcgtg
    15841 gatcggataa atttctgcaa gcactgttcc tttggctagt tctgcttgtc cctttgtttc
    15901 ttcatttaaa tgaaccgttc cagatacagg agcttttagt attccttttg aaagttgatc
    15961 gtccaaactt ttgataactg actggttctt ctcttgtttt tcatttagct ctgtaactgt
    16021 ttgttttaca gttgttattg cttgttctag ctcttgcttt cctttttccg tttgactaat
    16081 tctgtcattc tcagaagtag ttggtttaac taatttagct ttttctgact gatactgttc
    16141 tacttctttt tttagttgct caatttgttc atttatgctt gctgaaattg tcagtttcac
    16201 ttgatttttt tgatcatcag aaacattatt tagttgttct tgccaattct catagtttgc
    16261 catcatttct tttgaaaaat cttttaatga ttgattatta ctccaagcta cctgtacttg
    16321 ttgccattct tgtaatttac tatctgaact ctcaatttgt ttttctagtt gtctttttgt
    16381 cttttcgtat acttctaatt gcttttgatc atttaattca ctttgcttca aagcgtagcg
    16441 gagtgattct ttttctgaca acatgctctt taattgatta ctgtagccaa aactgtcatc
    16501 cgttgaaaac aaattttttc ctttacttat actttcaata aatagctgag ccatctttat
    16561 ttgttgagta atacgttggt tttcttgttc tatctgagcc ttttcattct ctattaaaga
    16621 acaatccaat ctaaccaaca catcgtcctt atgaacaaat tgattttctt ttaggaagtt
    16681 ttccattatt ttagcttcta atggcacttg taacttctct attttttcgg ggttcaattg
    16741 agccgttgtc cgaataacaa cctctttttt agcaaaggtt aaaaacaatc caataataca
    16801 taaaaaaaga attacagggt ataacaccca acggtagaag ctatgatgtt gtttagtata
    16861 gatactagaa tgttcgaacc aattattatt ctgcataaat tgtttccctc tcctagattt
    16921 cccataattt ttgatagata ccatcggcaa aacgaagttc atcgtgcgtt cctttttcta
    16981 ccaaatgccc ttcatggaga actaaaattt gatcacatgc ttttgctatt ggtaaatgat
    17041 gcgcaataaa aataatcgtt ttttctttta aattgaggag ataattcata attgcatgtt
    17101 ctaacaaggt atctaatcca ctagttgctt catctaaaat taatatgtcc ggatttttta
    17161 ataaagcccg tgctaaggct aatcgttgtc gttgccctcc tgaaagatta tttccacctt
    17221 cttccaacaa tgtctcgtaa cgtaatggct gttgattaat aaattcattt agttggacta
    17281 tctcacaaac ttctattatt tgttcgaaac ttggtgggct atctaaaccg aatataagat
    17341 tctcaatgat tgttccacta aagaaaaatg actcttgagg caaatatgtc actcgatcgc
    17401 gtaatatctg aaaaggaatg tctaagtgat taatgtttcc ataatggatc gatccttctg
    17461 atgcttcata aaaacgaaca aataactttg caagagttga ttttccagaa ccactaactc
    17521 caactatagc cgttttactg tgcgaaggaa ttgtacatga aatttcgttc aacgttgggg
    17581 ccttcatgct atacgagaat gaaacgtttt ttacagtgat atcttgttga aataattgtt
    17641 cagaaagcat atattcaaaa gactggttcg atttttctga atcaatcgcg aaaatttcat
    17701 ttaaacgatt atttgcaact tgagccgttt gcattttcac ttgtaaatta ataatgtttt
    17761 gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt gataagttga cctagactaa
    17821 ttctattatc cataacaaaa taagaaccta gccataaaat taatgcactg ctaattaatt
    17881 gaatcaaatg tttaattcct tgttgaatat tatcaagcgt aaccgtacga aatgattttt
    17941 tcattaattt aacaaattca tggtctatac ggttatatac tttctcttca ccgctatatg
    18001 ctttaattgt ttctattcct ttaagacttt ctatgatact agaatttaca gctgcaccgg
    18061 ctgccatttc ttccctgttc gatttttcat agcttttcac gaaagcgaat attgcaacta
    18121 cataaaaagg caaagagcat agagtgatta aaaacaacgt actattctga atagctaacg
    18181 taaccccaac aagcaacacc ataccaatat ctaaaaatat tgataacgta gcacttgcta
    18241 aagcatcaat aatcttactg gcatcaagaa aacgagaaat aatttcccct gattttctcg
    18301 tagcaaaaaa attcattggt aaagttaaca catgtttgaa atactgaagc ataattgcca
    18361 tactcattcg ctgtcctaaa ataataagaa gataactacg actatattca aacaataccc
    18421 gaaataaata gacagcgatc aagccaatgg aaattatatt taaagttgaa cgtgcttgat
    18481 tagggatgaa aaaatcaaga ataccttgaa aataatatga gcttcctatg ccaaaaaacg
    18541 taatgaaaat cgatgctaaa acgatgtgga atactaatcc tttttgtttc caaattatag
    18601 gcaaaaaaga acttaatcca gctacttttt ctttaatagg aatataagtt tctctaggca
    18661 taggtaataa aagaatacct gtccattctt tttgaaacgc ttcaatcgtt tttctatttt
    18721 ttccattagc gggatctgca atccatagta cgtttccctt cgttttataa attacaacgt
    18781 agtgcatata gctgttatca accaatatat gtgcaattaa tggtaagata agatctcttt
    18841 ctttccagat ctcactatct gcttgaatag ccatacaatc aaaagacaat ttttcaaatg
    18901 tctttttcaa tccaaatgct gaagtaccct ctaaatcagt cccagatagg tcccgtaatt
    18961 tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat cgaaaggcaa gcaacaccac
    19021 aatctttttc atcttgttgc tgcacaaaag gaaatgattt tatcacttat ctaacacaac
    19081 tccttctaaa aaatttttat agaaaaattc tcaatatagc tttaatacag tgaaaatatt
    19141 gtctcataat tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg
    19201 aatcaaattt attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga
    19261 tttttactgt taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga
    19321 agcgtgttta aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt
    19381 ggtggaggca ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg
    19441 gcagtaggtg gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga
    19501 gggttatatg gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa
    19561 aaaaggagaa taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca
    19621 gaagagttga aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct
    19681 ggggcaacag caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt
    19741 ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt
    19801 aaaaaatatc aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat
    19861 cttttcaaaa gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat
    19921 aattatatta aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg
    19981 gtagagatat ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat
    20041 ttcggccgtt acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc
    20101 tgcaagttaa atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca
    20161 taggattgca cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa
    20221 ttagtagaat ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt
    20281 tatggtactt aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac
    20341 catagaaata ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat
    20401 aaaaattggt acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt
    20461 tattagaaaa atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt
    20521 ctccttaatt ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata
    20581 tagtgaacgt tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg
    20641 actaattctt gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc
    20701 gtactgccca tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa
    20761 ataaatgtca tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg
    20821 attcgtaacc tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat
    20881 ctcgtttcga tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct
    20941 tctttttatt gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg
    21001 aacatcatat tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc
    21061 aatatctaag aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc
    21121 taacggactt tgtgttttca atactatctt aaatacttta tcgtgaaaaa gaatgaaatt
    21181 atcaataatt cgttctaact gatttaattg tataatttga tcttcacata atattattgg
    21241 ataactcata aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac
    21301 ggatcgtttt gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta
    21361 gataaaccta acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct
    21421 atagagagta gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct
    21481 cggtaaatag ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta
    21541 tcgagaataa ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata
    21601 gtacttggac attcgaattt acaacaaatc ttattgttat ttgcttggta tagcttcgct
    21661 atcaacaaac ttttcaaata ttcatttttt atatttttta aataacgata ttcaaactta
    21721 ttggttagat aagaattaga atagatctct aattctttta tttctttttc caatatcgaa
    21781 tcatggtttt gattagcgat ttcttttaaa ctcagcaata aatttttata gtcatgtcta
    21841 aatctggcaa atttttcttg atctttttcc agtgaatcag tatatttttt tagataaacc
    21901 aattcttttc ttttcaattg ctgttcatat tgttctcgtt gcctaatagt tattctaata
    21961 aataaaaata gaacaacaaa catttgaatg attaaaaata ttaagacacc aaacacaaaa
    22021 tgatcaaata cttgatattg atgagcaaca taagaaacaa ataaaccaat tgtaagtaaa
    22081 taaatcatga atatagcagt aagtttagaa ctggactgtt caacaatcct atttatagct
    22141 aattttctat ataaccaaat aaacatcagc aaaaacaaag aatcaagcat tatctggaat
    22201 actacataac catacccttt tatcccttca aatgaaaaca tatacgtcat aaccatggaa
    22261 gtaataatag atctgaaatc atcaataagc atacataata gtaatttatt taaaagcact
    22321 atattagttt tcttcttttc ctttaatata aaataacttc cgattatagg tatagctgcg
    22381 gaaacatcag taaataattc aacaaaaaaa gtgagtggta tcaataaaca tacaaaaaga
    22441 tattttcttt tgttaagatc agtattagtc atataaaata agagaaaaaa gaaaaagttt
    22501 cccacgaaca ttcttaatag ataagctttt gtatcgatag taatcaagag atcccctcct
    22561 gattttagga tatcataaaa aaaattttag ttacactttt taaataatga aaaaacacat
    22621 tccactaaat ttgatgccgg ttttccttga ggttttgttc cagcacctcc tttaattttt
    22681 ttaagctctt gatctgaaca ttgtttagca tttagtctat ttttttcttc cattacgatt
    22741 ccccctacaa actataaata cttttatgtc actgattcaa tatacctatt tttctaatca
    22801 aaaaattttg agacatattt aatattataa gtatcattaa aattgagatt tatctccata
    22861 atctgctcga tataaaccat ttattccagc agttcttcca atttcactca aatttcttat
    22921 taattcctct tgatattcag tgaatcttat tttattagta taagcagtaa aatatagata
    22981 attcaccaat ctatttatta aaggaccggg atctaattca tatttttgtt tttctaattt
    23041 taaatatacc ttttgtaaaa cctcaagaat atcagaatat ttaggatctt tacttataga
    23101 tatatcatta tataattcat gaacaatttg cttagcattt tttttcattt tagcacttcc
    23161 ctggaattgc tccacctaaa aaaccaccta tagacattcc tgcaatacaa gtagttgcct
    23221 tggcccaatc gaccgtacat ttatttttag tgcaatacac tccatttcca taatattttc
    23281 cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa atttttaaat
    23341 gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta acattcatta
    23401 aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat aagaatttaa
    23461 tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat gcacttcgta
    23521 cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt tgactcgcac
    23581 ccataaattc ataatagttt gtcggcgtct ttggacgttt cgaagatgag ctacgcattc
    23641 tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt tggatatttg
    23701 atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc cagctttcga
    23761 ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct tttccattgt
    23821 tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg tctaatactt
    23881 tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg atctgctctt
    23941 cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg gcaaacatcc
    24001 aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct tgacctagtg
    24061 caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag ttgtcatatt
    24121 tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt cctatgattt
    24181 tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag gctggcactg
    24241 ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga tctttatatt
    24301 gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca tctgactcgc
    24361 catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt acatcatagc
    24421 ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta tcccattcaa
    24481 cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct ataccaatcg
    24541 cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt ttacttttct
    24601 gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt gtaaaacggt
    24661 caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc ccgacttgtg
    24721 ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa
    24781 taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg atcgtacttt
    24841 tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg aatgaatcag
    24901 aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg
    24961 gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata ccgaaaaatg
    25021 caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca aggatcggtt
    25081 tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt accccaatga
    25141 tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa tcattccata
    25201 gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt cctggacgtt
    25261 tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga gctggaatca
    25321 ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa
    25381 ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag tttgccactg
    25441 gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc tgctaatgct
    25501 tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac aactgcaagt
    25561 ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc tttcccaatc
    25621 accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct agcttctaac
    25681 gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc catcagcatg
    25741 ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc
    25801 gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg atcgacttct
    25861 tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc acctgttccg
    25921 atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg taaagcttca
    25981 ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag gtccagtaat
    26041 tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc gtccatcagc
    26101 aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg catcccacca
    26161 gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc taatgctttt
    26221 tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc gtattctgta
    26281 ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat attgatttta
    26341 tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc tccatcaata
    26401 tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa tgtggatttc
    26461 ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc aaaattcacg
    26521 tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg ctggtgatca
    26581 ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa atgttttact
    26641 tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt tctgaaaaaa
    26701 gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga aatttgcttg
    26761 tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc aagttaatat
    26821 ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa aaagtttgtc
    26881 atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc cgtaaattta
    26941 gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat aactgtggcg
    27001 ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat tgctttttct
    27061 gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt cgtttgattg
    27121 accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt ctccacactt
    27181 gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg caataggaaa
    27241 tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac ttccatgatt
    27301 ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc attgtacgta
    27361 ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt ttcaacgata
    27421 cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt tttctttcca
    27481 gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct ctagcaaaat
    27541 aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat ctgttccttt
    27601 tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa acgagtatag
    27661 acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc catcctattc
    27721 ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct gacggaacaa
    27781 aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg ctacctgtcc
    27841 accctccaat aagtgtccca actgataaat cacaaaacta actaaataag ctaaaccgca
    27901 ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat ggatagcccc
    27961 aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat aagctgctgc
    28021 gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg atacttccgc
    28081 atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca atcctgtaat
    28141 cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa ctgccaggat
    28201 tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat aaaaattata
    28261 agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg cttttttaac
    28321 aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag gaaaatgata
    28381 tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg tttttttcaa
    28441 gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag gagctaccca
    28501 agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact ttgcagaaca
    28561 aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg
    28621 actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa tgaaggattt
    28681 tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc tcgccatata
    28741 tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg gtagaaatcc
    28801 taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca tccatcccgc
    28861 tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac caaacaatgt
    28921 gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat aatagatcag
    28981 ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat
    29041 tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg tacatagttg
    29101 ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt ccaatagttt
    29161 ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta tctcgtaatc
    29221 ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt tcttgccgct
    29281 ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg gatcatatgt
    29341 acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt cgattagttt
    29401 ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa gaccatagga
    29461 aagcttttct agattgattt tttttccttc ttttttgatg acatccatca tattcagacc
    29521 aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat ttcgttctaa
    29581 attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca aataatctct
    29641 tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta ggtcctgaat
    29701 atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta cgcccggcca
    29761 attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt tcccgctgtt
    29821 tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa gattagttcg
    29881 gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat tggatctccc
    29941 agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat atccattaac
    30001 cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc tcctgctttt
    30061 aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac aaagatttga
    30121 cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat ggcattgttg
    30181 cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt ttgcaaatgg
    30241 cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc
    30301 tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat taaaatcatt
    30361 cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat tctcacatag
    30421 aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg aatctttagt
    30481 attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat tttttctcta
    30541 ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa aaacacaaaa
    30601 cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga cttttttact
    30661 aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg
    30721 aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta
    30781 acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac
    30841 tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt
    30901 atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa
    30961 aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa
    31021 atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga
    31081 aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc
    31141 tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag
    31201 ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt ctatctatta
    31261 tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc attcaattgt
    31321 attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca
    31381 aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt
    31441 ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg
    31501 gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag
    31561 ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct
    31621 tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat
    31681 tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc
    31741 ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag
    31801 atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga
    31861 acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa
    31921 agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga
    31981 cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg
    32041 tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca
    32101 ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag
    32161 aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg
    32221 gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga
    32281 ttaacggcaa acaggaatat gaagtattag gaacggatat ctcatgtaac ttaggctcga
    32341 caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc
    32401 gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg
    32461 gaaataagct gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa
    32521 aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc
    32581 tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt
    32641 tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg
    32701 gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa
    32761 ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa
    32821 atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta
    32881 ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt
    32941 acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg
    33001 acatgcgtaa agtaatcgat gtttatgcaa tggcacaaca acacgtcgat caaggcatga
    33061 gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa
    33121 cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca
    33181 aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat
    33241 gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg
    33301 agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc
    33361 attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca
    33421 ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga
    33481 catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag
    33541 ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg
    33601 gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa
    33661 atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt
    33721 atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta
    33781 gaaacattct tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa
    33841 ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc
    33901 tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg
    33961 ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca
    34021 gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct acgctataat
    34081 gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat
    34141 gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca
    34201 caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg
    34261 attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt
    34321 agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt
    34381 ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga
    34441 ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca
    34501 tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat
    34561 ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga
    34621 tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa
    34681 tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc
    34741 aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca
    34801 ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt
    34861 ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca
    34921 acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa
    34981 ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac
    35041 tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt
    35101 ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt
    35161 catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca
    35221 ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac
    35281 cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat
    35341 agataaaacc agcaacaagc aatggtaaaa aaactgccga aagcattttc aatattggac
    35401 taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca
    35461 ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc
    35521 ctttgatact tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt
    35581 aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac
    35641 ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa
    35701 aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt
    35761 ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc
    35821 aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc
    35881 ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc
    35941 atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata
    36001 atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta actttttagt
    36061 cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact
    36121 gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca
    36181 taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc
    36241 gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat
    36301 cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac
    36361 ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc
    36421 tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc
    36481 taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag
    36541 tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa
    36601 aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca
    36661 gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct
    36721 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt
    36781 acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata
    36841 cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt
    36901 tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca
    36961 ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga
    37021 ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat
    37081 tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat
    37141 cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta
    37201 caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc
    37261 tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac
    37321 tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg
    37381 ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt
    37441 ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt
    37501 ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa
    37561 ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg
    37621 ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta
    37681 tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt
    37741 acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc
    37801 gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc
    37861 actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat tcacaaaggt
    37921 tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact
    37981 gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc
    38041 gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa
    38101 ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat
    38161 gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt
    38221 ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac
    38281 ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat
    38341 atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc
    38401 gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa
    38461 caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc
    38521 gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa
    38581 aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt
    38641 gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc
    38701 ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt
    38761 gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact gatcggtgag
    38821 attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca
    38881 caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt
    38941 ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa
    39001 ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca
    39061 cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat
    39121 caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc
    39181 gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca
    39241 aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac
    39301 tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg
    39361 caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt
    39421 cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg
    39481 tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc
    39541 catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca
    39601 taaacataaa tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg
    39661 tttgattggc gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca
    39721 aaatggccaa atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa
    39781 gtcccatata cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg
    39841 cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat
    39901 tgtaggcggg actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa
    39961 acatgaaaaa tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg
    40021 gcaaactttt aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg
    40081 gcattttcca gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga
    40141 tcatccgcca agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc
    40201 ccacgagtaa accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta
    40261 gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag
    40321 aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag
    40381 ataacgctgc cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta
    40441 aaaacagtaa ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac
    40501 ttgtgctaac agataaacta gctgctactt ttgttccaat cggctggatg taatacatat
    40561 tggcaacaac aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtt
    40621 ttttttcatt actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa
    40681 aagattggaa atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc
    40741 cttgtttttt cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac
    40801 ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg
    40861 catacaacac agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag
    40921 gcaatttcat cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat
    40981 agcctaacat tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat
    41041 tcgtttcggt tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt
    41101 taccaagagc attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac
    41161 cagcaatgac tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca
    41221 atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg
    41281 ccttactcgc tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc
    41341 gatagcccac taccagtatc agaatgattc ccaacgagat caatatcaat cggattattt
    41401 tctttttcat atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa
    41461 aacaaaaaag caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt
    41521 taacagttac agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt
    41581 tagatacgcc ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca
    41641 agcctaaacc agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg
    41701 cagttaattc tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag
    41761 ctacagctac aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt
    41821 cgttaagttc taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg
    41881 ccattataat ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa
    41941 gctgcatctc catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact
    42001 ggcgcttgta gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc
    42061 aatgctgtga tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct
    42121 aatgcttttg cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg
    42181 ttactgaatg caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct
    42241 tttttagctg cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca
    42301 cgaagttgtt tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact
    42361 actactgatg cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt
    42421 gcgataattg cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt
    42481 tgcactgaaa aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc
    42541 tattcacttc gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt
    42601 gctcaaccag tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac
    42661 tctatgccac cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg
    42721 aaattaaggc ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag
    42781 cttagcatgc taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct
    42841 aatatttaat tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat
    42901 cgttacattt ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc
    42961 atggattgct gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg
    43021 tacatggata tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt
    43081 tactgctttt gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt
    43141 tggtcctaat actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac
    43201 tacatcaaag tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa
    43261 atctgcacca gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt
    43321 ttgtgtttta ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt
    43381 tttagggtct acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt
    43441 tgtttctttt gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt
    43501 ttttaatgca tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg
    43561 gttataacgg ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag
    43621 ctgctgtctt agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt
    43681 caaccatgcg cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag
    43741 cgatttctct tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg
    43801 aacctttttc gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa
    43861 cgaatgtgaa tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt
    43921 gatcagctgt acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac
    43981 ctagtgcagg acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa
    44041 cgattttttc tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat
    44101 tggagatgca tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat
    44161 gtgcaaaaca atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt
    44221 tctgctattt ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt
    44281 tttcgttctc atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag
    44341 caaacaataa cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt
    44401 ggagaaaatg aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat
    44461 tggtccaagt agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat
    44521 atttggcgaa cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta
    44581 tcgggggcac ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga
    44641 tgaacgttta gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt
    44701 tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac
    44761 acgaaagctt tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt
    44821 gaatggattc aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga
    44881 tgttccaggt atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg
    44941 cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga
    45001 tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt
    45061 aaattatttc gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca
    45121 agcaaaagct tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg
    45181 ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc
    45241 aatcaacgag ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa
    45301 aaaattagat gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc
    45361 tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac
    45421 accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct
    45481 tgatttgaca catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt
    45541 catcgcaaac aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc
    45601 tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc
    45661 agcacaagca gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc
    45721 aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat
    45781 ctctgctgat atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc
    45841 tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg
    45901 cttagctgtt acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg
    45961 ataaagctcc ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga
    46021 gaggactaat tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt
    46081 catcgatcgt ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt
    46141 caaaaacaga tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc
    46201 caatcgcaac taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga
    46261 caccagagtc gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata
    46321 cttcaaagga cgcaggacct ccatctagtt gacgtccacc atgattggat acccagatac
    46381 catctgcccc tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga
    46441 taactggaag atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt
    46501 ttgcagctgc ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt
    46561 cgctatacgc agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt
    46621 cttcacggta accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt
    46681 ctactgcttt gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga
    46741 aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag
    46801 tgctgatgga aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt
    46861 tttcatgtgc caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa
    46921 ttccgaaaac agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga
    46981 ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac
    47041 cgcgaatata gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg
    47101 caatgtttac aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc
    47161 cttctttcat cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca
    47221 tttactctca gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct
    47281 acttcttggt tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc
    47341 tctatttcat ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt
    47401 actgttccat cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg
    47461 gttataggaa tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca
    47521 ggcccctttg ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa
    47581 ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt
    47641 cctaaatagc gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct
    47701 atgccgtagt tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac
    47761 tcgttatttt tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac
    47821 aaatctcctg ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact
    47881 gcttgctgat ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg
    47941 tagccgtgga aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc
    48001 gttttttctt tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac
    48061 gcaaacaaac tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt
    48121 ggttctgtca tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc
    48181 atatgatcga catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag
    48241 ttctgtactg gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc
    48301 gtcaacttct gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac
    48361 ttcgccaact tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat
    48421 atgattgact tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac
    48481 gaatcctgtc acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat
    48541 ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt
    48601 gacttcagtt tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat
    48661 actttgcgca cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg
    48721 caagacatac cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat
    48781 aaaaaaacct cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt
    48841 tgtggctagc gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta
    48901 cgtaaaacat caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga
    48961 gttgttttct gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt
    49021 tcatgagaca acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt
    49081 acaatattta caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga
    49141 ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat
    49201 ttacttctgc ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt
    49261 ttacttgtca atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta
    49321 atttgaaatt aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg
    49381 tatataagaa aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa
    49441 aaattttaga aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt
    49501 tccgtggaaa catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga
    49561 cgcttcctgt cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt
    49621 tcttcaaagt attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa
    49681 atcattttta tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga
    49741 gcgtagaaca aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg
    49801 ctgctattgc aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata
    49861 tcgaaaaaga cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga
    49921 aagtatgtga aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg
    49981 aaaatttgca aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct
    50041 ttcttttttc gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata
    50101 atacatcaat ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata
    50161 ggttgtccaa aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct
    50221 gatggtaaag aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg
    50281 caataaagct gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat
    50341 gataccaaaa ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa
    50401 tttacggtta aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc
    50461 tgaggataca ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt
    50521 tacaacgatt acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt
    50581 ctcctagttt ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa
    50641 ttataacaga cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct
    50701 taaacaatgt aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt
    50761 cataagaatt cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa
    50821 cttaaataaa taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag
    50881 caagcagctg aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa
    50941 tggatcatgc gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc
    51001 ctagacttct gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa
    51061 gtttctttca ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga
    51121 gtattaaaat atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata
    51181 tattgatcaa tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac
    51241 cggtattaaa aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg
    51301 atgctgagat tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac
    51361 agaaaacaat cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc
    51421 aggtacttga tactttctat cagatggaag tagaattccc agttgcttat attccagcag
    51481 gttctggaga tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc
    51541 agctgatttt agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa
    51601 taagtgaaaa aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg
    51661 tccatgcgac aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat
    51721 tttcgtattt attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg
    51781 tagatgcagg aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc
    51841 atcctttttt tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca
    51901 ttgattttgt cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac
    51961 ttatacagaa aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac
    52021 gtatcgtttc aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac
    52081 cttttgatat cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt
    52141 gtcagaagct acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg
    52201 tcacagtcct ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt
    52261 ttctttcttt acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga
    52321 cacaaccatt gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga
    52381 tcaagttatt attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca
    52441 tttctggcaa gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa
    52501 aaatatcttt gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg
    52561 aaaagctaat gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta
    52621 tatcccacga ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca
    52681 gcagcgagta actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg
    52741 gcgcactctg gtttcgttta ctgatctctt tgcgattaaa tgctagattg atgacgctga
    52801 aaccgctaaa aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca
    52861 acagcttttt catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga
    52921 gtgccatttg tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa
    52981 tcagtgtttg caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg
    53041 ggcgactctg tatcttgatc gtcggcatct ttcctacttc gacttgttcg gtaaagtctg
    53101 caggcagatg taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt
    53161 gtgaggttcc atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat
    53221 cgactacagg cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat
    53281 tttcaacatc aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta
    53341 tcagtaaatt cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa
    53401 cgcacccctc tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac
    53461 catagaagat tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt
    53521 tcgctgatca agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa
    53581 tacttcactt gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa
    53641 atcaagatgc tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta
    53701 cctaacaaaa tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt
    53761 tcaccaaagt ctacttgata gataaaacga aagacagcta acataatata aatctctgtc
    53821 aaaacaatcg taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga
    53881 atcaaggaca agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg
    53941 accccccaca tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt
    54001 ttgaaactca tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca
    54061 gtcatgatgg gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca
    54121 atacgctgta aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga
    54181 cccacatgga gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca
    54241 tttagctttt cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca
    54301 ttgacttcga cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct
    54361 ttgattacaa ttccagtatt aatcgtttcg agattgccac cttgatcaat ctcgccaaag
    54421 gccgccataa acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat
    54481 ttatttcgca gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct
    54541 cctctagtct ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc
    54601 tttggttgct atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa
    54661 caatactgta tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc
    54721 atcagaagct attgttcgct gacaacacgt tttctgggag taatgggatg tcgatcgtca
    54781 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    54841 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    54901 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    54961 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    55021 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    55081 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    55141 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    55201 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    55261 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    55321 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggtcctaac agacctaaga
    55381 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    55441 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    55501 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    55561 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    55621 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    55681 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    55741 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    55801 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    55861 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    55921 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    55981 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    56041 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    56101 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    56161 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    56221 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    56281 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    56341 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    56401 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    56461 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    56521 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    56581 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    56641 ttaccaaggg agggacacca atgataacag tagtcattat tgagatgatc catttgtgac
    56701 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    56761 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    56821 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    56881 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    56941 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    57001 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    57061 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    57121 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    57181 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    57241 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    57301 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    57361 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    57421 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    57481 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    57541 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    57601 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    57661 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    57721 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    57781 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    57841 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    57901 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    57961 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    58021 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    58081 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    58141 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    58201 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    58261 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    58321 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    58381 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    58441 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    58501 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    58561 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    58621 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    58681 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    58741 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    58801 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    58861 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    58921 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    58981 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    59041 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    59101 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    59161 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    59221 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    59281 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    59341 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    59401 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    59461 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    59521 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    59581 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    59641 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    59701 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    59761 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    59821 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    59881 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    59941 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    60001 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    60061 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    60121 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    60181 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    60241 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    60301 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    60361 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    60421 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    60481 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    60541 tacgaataaa agtgggccat ctttatgttg ggcaaccgca tctgctacaa cagtttcgat
    60601 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    60661 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    60721 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    60781 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    60841 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    60901 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    60961 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    61021 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    61081 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    61141 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    61201 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    61261 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    61321 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    61381 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    61441 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    61501 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    61561 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    61621 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    61681 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    61741 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    61801 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    61861 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    61921 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    61981 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    62041 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    62101 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    62161 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    62221 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    62281 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    62341 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    62401 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    62461 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    62521 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    62581 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    62641 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    62701 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    62761 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    62821 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    62881 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    62941 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    63001 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    63061 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    63121 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    63181 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    63241 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    63301 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    63361 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    63421 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    63481 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    63541 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    63601 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    63661 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    63721 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    63781 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    63841 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    63901 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    63961 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    64021 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    64081 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    64141 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    64201 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    64261 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    64321 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    64381 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    64441 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    64501 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    64561 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    64621 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    64681 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    64741 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    64801 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    64861 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    64921 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    64981 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    65041 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    65101 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    65161 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    65221 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    65281 caaatccatg ctttgcaaaa agcatcaaac cagatgtatc ccgatctagt cgcgtcacca
    65341 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    65401 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    65461 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    65521 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    65581 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    65641 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    65701 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    65761 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    65821 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    65881 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    65941 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    66001 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    66061 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    66121 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    66181 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    66241 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    66301 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    66361 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    66421 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    66481 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    66541 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    66601 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    66661 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    66721 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    66781 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    66841 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    66901 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    66961 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    67021 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    67081 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    67141 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    67201 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    67261 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    67321 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    67381 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    67441 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    67501 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    67561 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    67621 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    67681 attaatccaa accggaaagc ttacaacaaa acgggctgaa ttccctattg aagaaggact
    67741 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    67801 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    67861 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    67921 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    67981 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    68041 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    68101 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    68161 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    68221 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    68281 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    68341 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    68401 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    68461 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    68521 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    68581 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    68641 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    68701 taattaaaaa aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat
    68761 tcgagaaaat caaaaataga aagccttata aacgatcgat caattcttct aattcattta
    68821 gacgttgttc aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc
    68881 ctgctttttt cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt
    68941 agttctctag tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag
    69001 aaaagccagt cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc
    69061 attcgaattt aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt
    69121 taccataact atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat
    69181 gcatgaaatg ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat
    69241 ccaagtaatg gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat
    69301 actccgttag gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt
    69361 cgccgtacac ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt
    69421 cgtggacaag cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat
    69481 ttgtgtcata gcttcccgaa gaataagcat cgcttcgttt ccctttattt tcgacaacat
    69541 cgatccaacg ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt
    69601 tcagtgcttc taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc
    69661 ctaagaccgg tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc
    69721 gtaattccat gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat
    69781 cgtatacact ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat
    69841 tacggacttt cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg
    69901 tatttctaaa ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg cgcacccttc
    69961 gatccgtgct ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat
    70021 tttctccttc aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac
    70081 ttgaagcatc aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga
    70141 catgggctcg attatctact atttgctgga tataatggcg atagacttcc aattttggtt
    70201 cttctttgaa atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc
    70261 aggaaacggc ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt
    70321 aatctgtatt tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag
    70381 tttggcgata aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat
    70441 caagtgttcc tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct
    70501 catcaaactc ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca
    70561 attcttctcg ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag
    70621 ctctatctat caaaatagat catagtctaa catccacatg tcacagcatc taacttttct
    70681 actcattcat gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa
    70741 aaccgattcc tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat
    70801 tcgttaaaaa actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt
    70861 ttatgttctt ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag
    70921 gcttcttcgt acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga
    70981 cttttttgat agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt
    71041 tcttgtatgg atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc
    71101 ccatcttcac tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg
    71161 aatatttctt taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc
    71221 cctgatacag tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct
    71281 ttccagcaat gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag
    71341 aattgcgatt gtccaagaaa agaattacca agtatccacc atacttggta actgttattc
    71401 aaataatttt gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct
    71461 attgctagat tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc
    71521 aaaaatactg gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag
    71581 tgctcttttg attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa
    71641 tgagcacatt ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa
    71701 tatttctttc ctttttctgc atgtttagca aaacagcact tattatattc attcattgca
    71761 agcagcattt ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag
    71821 agggcgtgaa aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc
    71881 aatatttttc gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac
    71941 gaaaaaaaga ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca
    72001 aaataaaatt tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc
    72061 aatgtttctg gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc
    72121 tctacatccg cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct
    72181 tcgaataaat acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc
    72241 acttgttcaa aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt
    72301 ccagataatt cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca
    72361 tcttctgaat caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta
    72421 ctgataaaca gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt
    72481 cctcggaact cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt
    72541 tctttgtgat tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga
    72601 ccaattagta cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc
    72661 actttgccac aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa
    72721 aaaaatctcg ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta
    72781 tcttatttaa ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat
    72841 tttccctctc tatttttcgt gcaatcatca tcttctacta cctttttata catctaatca
    72901 agaatacttt ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat agtcttt
//
LOCUS       DAPBCS010000006        65587 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPBCS010000006 DAPBCS010000000
VERSION     DAPBCS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 65587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.006"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..672
                     /locus_tag="RD521_000524"
     CDS             1..672
                     /locus_tag="RD521_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7770285.1"
                     /translation="MQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTPIREAL
                     IQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQDQVKLE
                     KILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFRWLRLT
                     ISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFEDIQ
                     DRPIK"
     gene            complement(1080..1769)
                     /locus_tag="RD521_000525"
     CDS             complement(1080..1769)
                     /locus_tag="RD521_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770286.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(1744..3873)
                     /locus_tag="RD521_000526"
     CDS             complement(1744..3873)
                     /locus_tag="RD521_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDT7770287.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(3921..5258)
                     /locus_tag="RD521_000527"
     CDS             complement(3921..5258)
                     /locus_tag="RD521_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDT7770288.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(5510..5869)
                     /gene="rplT"
                     /locus_tag="RD521_000528"
     CDS             complement(5510..5869)
                     /gene="rplT"
                     /locus_tag="RD521_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDT7770289.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(5922..6122)
                     /gene="rpmI"
                     /locus_tag="RD521_000529"
     CDS             complement(5922..6122)
                     /gene="rpmI"
                     /locus_tag="RD521_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDT7770290.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(6194..6718)
                     /gene="infC"
                     /locus_tag="RD521_000530"
     CDS             complement(6194..6718)
                     /gene="infC"
                     /locus_tag="RD521_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDT7770291.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(6762..6887)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            7038..7670
                     /locus_tag="RD521_000531"
     CDS             7038..7670
                     /locus_tag="RD521_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDT7770292.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(7672..8577)
                     /locus_tag="RD521_000532"
     CDS             complement(7672..8577)
                     /locus_tag="RD521_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7770293.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(8662..9321)
                     /locus_tag="RD521_000533"
     CDS             complement(8662..9321)
                     /locus_tag="RD521_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7770294.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            9637..10581
                     /gene="mvk"
                     /locus_tag="RD521_000534"
     CDS             9637..10581
                     /gene="mvk"
                     /locus_tag="RD521_000534"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDT7770295.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            10574..11551
                     /gene="mvaD"
                     /locus_tag="RD521_000535"
     CDS             10574..11551
                     /gene="mvaD"
                     /locus_tag="RD521_000535"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDT7770296.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            11564..12649
                     /locus_tag="RD521_000536"
     CDS             11564..12649
                     /locus_tag="RD521_000536"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDT7770297.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            12646..13710
                     /locus_tag="RD521_000537"
     CDS             12646..13710
                     /locus_tag="RD521_000537"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDT7770298.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(13959..14762)
                     /locus_tag="RD521_000538"
     CDS             complement(13959..14762)
                     /locus_tag="RD521_000538"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDT7770299.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(14782..15624)
                     /gene="kduI"
                     /locus_tag="RD521_000539"
     CDS             complement(14782..15624)
                     /gene="kduI"
                     /locus_tag="RD521_000539"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDT7770300.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(15640..16647)
                     /locus_tag="RD521_000540"
     CDS             complement(15640..16647)
                     /locus_tag="RD521_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDT7770301.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(16654..17298)
                     /locus_tag="RD521_000541"
     CDS             complement(16654..17298)
                     /locus_tag="RD521_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDT7770302.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(17398..18156)
                     /locus_tag="RD521_000542"
     CDS             complement(17398..18156)
                     /locus_tag="RD521_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDT7770303.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(18283..19434)
                     /gene="fucO"
                     /locus_tag="RD521_000543"
     CDS             complement(18283..19434)
                     /gene="fucO"
                     /locus_tag="RD521_000543"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDT7770304.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(19516..19827)
                     /gene="rhaM"
                     /locus_tag="RD521_000544"
     CDS             complement(19516..19827)
                     /gene="rhaM"
                     /locus_tag="RD521_000544"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDT7770305.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(19829..20674)
                     /gene="rhaD"
                     /locus_tag="RD521_000545"
     CDS             complement(19829..20674)
                     /gene="rhaD"
                     /locus_tag="RD521_000545"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDT7770306.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(20688..21944)
                     /gene="rhaA"
                     /locus_tag="RD521_000546"
     CDS             complement(20688..21944)
                     /gene="rhaA"
                     /locus_tag="RD521_000546"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDT7770307.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(21941..23401)
                     /gene="rhaB"
                     /locus_tag="RD521_000547"
     CDS             complement(21941..23401)
                     /gene="rhaB"
                     /locus_tag="RD521_000547"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDT7770308.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            23554..24423
                     /locus_tag="RD521_000548"
     CDS             23554..24423
                     /locus_tag="RD521_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDT7770309.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(24549..25448)
                     /locus_tag="RD521_000549"
     CDS             complement(24549..25448)
                     /locus_tag="RD521_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770310.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(25613..26437)
                     /locus_tag="RD521_000550"
     CDS             complement(25613..26437)
                     /locus_tag="RD521_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7770311.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(26448..27743)
                     /locus_tag="RD521_000551"
     CDS             complement(26448..27743)
                     /locus_tag="RD521_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDT7770312.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(27759..28238)
                     /locus_tag="RD521_000552"
     CDS             complement(27759..28238)
                     /locus_tag="RD521_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDT7770313.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(28244..29272)
                     /locus_tag="RD521_000553"
     CDS             complement(28244..29272)
                     /locus_tag="RD521_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDT7770314.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(29289..30395)
                     /locus_tag="RD521_000554"
     CDS             complement(29289..30395)
                     /locus_tag="RD521_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDT7770315.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(30624..33221)
                     /gene="adhE"
                     /locus_tag="RD521_000555"
                     /gene_synonym="adhC"
     CDS             complement(30624..33221)
                     /gene="adhE"
                     /locus_tag="RD521_000555"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDT7770316.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(33526..33906)
                     /gene="yajC"
                     /locus_tag="RD521_000556"
     CDS             complement(33526..33906)
                     /gene="yajC"
                     /locus_tag="RD521_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDT7770317.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(34003..35148)
                     /gene="tgt"
                     /locus_tag="RD521_000557"
     CDS             complement(34003..35148)
                     /gene="tgt"
                     /locus_tag="RD521_000557"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDT7770318.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            35365..36063
                     /locus_tag="RD521_000558"
     CDS             35365..36063
                     /locus_tag="RD521_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDT7770319.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(36231..38870)
                     /locus_tag="RD521_000559"
     CDS             complement(36231..38870)
                     /locus_tag="RD521_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7770320.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(39489..40520)
                     /gene="queA"
                     /locus_tag="RD521_000560"
     CDS             complement(39489..40520)
                     /gene="queA"
                     /locus_tag="RD521_000560"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDT7770321.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            40713..41888
                     /locus_tag="RD521_000561"
     CDS             40713..41888
                     /locus_tag="RD521_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7770322.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            41892..42533
                     /locus_tag="RD521_000562"
     CDS             41892..42533
                     /locus_tag="RD521_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770323.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            42530..43447
                     /locus_tag="RD521_000563"
     CDS             42530..43447
                     /locus_tag="RD521_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7770324.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            43444..44115
                     /locus_tag="RD521_000564"
     CDS             43444..44115
                     /locus_tag="RD521_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770325.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(44656..45144)
                     /locus_tag="RD521_000565"
     CDS             complement(44656..45144)
                     /locus_tag="RD521_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDT7770326.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(45345..45611)
                     /locus_tag="RD521_000566"
     CDS             complement(45345..45611)
                     /locus_tag="RD521_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDT7770327.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(45611..45874)
                     /locus_tag="RD521_000567"
     CDS             complement(45611..45874)
                     /locus_tag="RD521_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7770328.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            46350..46535
                     /locus_tag="RD521_000568"
     CDS             46350..46535
                     /locus_tag="RD521_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7770329.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(46677..48500)
                     /locus_tag="RD521_000569"
     CDS             complement(46677..48500)
                     /locus_tag="RD521_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDT7770330.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(49097..51802)
                     /locus_tag="RD521_000570"
     CDS             complement(49097..51802)
                     /locus_tag="RD521_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDT7770331.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(52108..53055)
                     /locus_tag="RD521_000571"
     CDS             complement(52108..53055)
                     /locus_tag="RD521_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDT7770332.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            53245..53514
                     /gene="rpsN"
                     /locus_tag="RD521_000572"
     CDS             53245..53514
                     /gene="rpsN"
                     /locus_tag="RD521_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDT7770333.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(53690..54052)
                     /locus_tag="RD521_000573"
     CDS             complement(53690..54052)
                     /locus_tag="RD521_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7770334.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(54063..55184)
                     /locus_tag="RD521_000574"
     CDS             complement(54063..55184)
                     /locus_tag="RD521_000574"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDT7770335.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(55200..55550)
                     /locus_tag="RD521_000575"
     CDS             complement(55200..55550)
                     /locus_tag="RD521_000575"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDT7770336.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(55680..57191)
                     /locus_tag="RD521_000576"
     CDS             complement(55680..57191)
                     /locus_tag="RD521_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7770337.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(57513..58865)
                     /locus_tag="RD521_000577"
     CDS             complement(57513..58865)
                     /locus_tag="RD521_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDT7770338.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(59018..60094)
                     /locus_tag="RD521_000578"
     CDS             complement(59018..60094)
                     /locus_tag="RD521_000578"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDT7770339.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            60290..62035
                     /locus_tag="RD521_000579"
     CDS             60290..62035
                     /locus_tag="RD521_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDT7770340.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(62315..62752)
                     /locus_tag="RD521_000580"
     CDS             complement(62315..62752)
                     /locus_tag="RD521_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDT7770341.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(62736..64109)
                     /locus_tag="RD521_000581"
     CDS             complement(62736..64109)
                     /locus_tag="RD521_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770342.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(64431..64919)
                     /locus_tag="RD521_000582"
     CDS             complement(64431..64919)
                     /locus_tag="RD521_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7770343.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(64941..65543)
                     /locus_tag="RD521_000583"
     CDS             complement(64941..65543)
                     /locus_tag="RD521_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7770344.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        18693 a        14196 c        11972 g        20726 t
ORIGIN      
        1 ttgcaacagc aagcttatga atctatacgt aaacaaatca tttattccga gttagcacct
       61 ggaagtaaaa tctccgataa agtgttagaa gaacaactag ctatcgggcg tacaccgatt
      121 cgagaagcac tgatccaact gcgcaatcaa gagcttgtaa aaaccattcc acagtccggc
      181 acttatgttt caaaaatcga tatccgatct gcttcattag cccgttatac gcgggaaaag
      241 ttggaaaaac cgatcctgca agaatgcagt gcgaaaatga ccaagcaaga tcaagttaaa
      301 ctagaaaaaa tactagaaca aaccgatcag gcagtaatcg ctcaaaataa aaaagctttt
      361 tttgatttgg atactgattt tcaccgaact tgttatgaaa ttgctggaaa acgagaaatt
      421 tgggattggc tagaaagtta tagtacgcac ttgaaccgtt tcagatggtt acgtttgacg
      481 atttcagaat tagactgggg tcgagtatta gatgaacatc agacaatgct tcaatcaatg
      541 attgatcata actttgatga agtcggattt ctttgcacga tgcatttaca tatgattatc
      601 gaggagcaag aatacgtgat tcataattac cccgattatt ttgaagatat tcaggacaga
      661 cccatcaaat aagaggtttt ggaatcagcg ttttgacctg agtattagag aaagaaacgg
      721 caaaatagtt cctcatattt tgccgttttt tttgagctaa taccgagggt cagctgattg
      781 cacaccgttt attgagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca
      841 agtaaaaata gcttcttatg tttttaactt ggctggactt attaccgcag atgcaagctt
      901 ttgaacaccg ttttccaaga ggtcgtgagc ctgccgttca tctccgagtc acaaggaaca
      961 atttcaaaaa acagcttctc atgtttgttg aatattttga cttgtgaccg aggagttgac
     1021 aggagaacac cgtttattcg gaaaaaaagg actgtgacac gacttttgtc acagtctctt
     1081 tatttgtaat ctaaatcata ctccgtacat cttttttcta ttttttttat ttcttctaag
     1141 atcccttggc tacgaatttc attatatggc caaagtaaga ctgcctctaa tacataaggc
     1201 gaaaaatcgc tttgattcat ctcttttgaa aaactcatcg ctgctatctc gctcaatgca
     1261 cgaatgtttg attgatgtgt ataaaaaggc aaccgccaca gcacaatttt ttcgctttgc
     1321 gtataaattc ccttaacaga agcttcgatg tggtgaaata gttcatgtcc aataatcaaa
     1381 gcttgcgctg tttctttaga gatcgaagct agtcctgaac attttaattt ttctaacggc
     1441 tccgtcatca atcgaatccc ttcatcttct gcataagttg caaaagtgat tcgttcgttt
     1501 atttgctctg attcacagga aacgattgga acagccattt gttcacaaat ttctcctaca
     1561 tcggcaaaag ggtacttttt tttgatgcta catgccgttt cgataccaca atcgattgat
     1621 tgttgtataa gaattttctg cttggacaaa ggaatctttt tccaaaataa atctcgccga
     1681 aactggtaga atccccaaat ttcttcgggg atctgttgta attcgtttag taactcttct
     1741 aacttatgtt tgtttgatat ttccggcaac gacgatcact tcttcctccg gttccatagt
     1801 agatacttcc agatccatca aaagctgtag ttgttccaga tccgtcgctg taaaatcaga
     1861 gactcttgct cctaagcaga gataaaattc tggtcgggca atcaattcag tttgataaac
     1921 tgccatatct tcccaaagtt gttcgacagc tgtcatataa ttggctgcag ttgttttcat
     1981 acatgaagca tgtccacgtt ggaccttgat aaatcctttt tgatcgatga tccgaagact
     2041 tccagcattt tttgaacgat tctgttcgcc gcatacataa aagaaaggtg tattttccaa
     2101 taagcaaact tcatttttat ttaatcgcat atcctctgca gcaagctcta gtgcttcttc
     2161 tgttgtgatc tccttggtta gatcggttgc tttcacttcc gtagaaccag tagcgatagc
     2221 cgttactttt gaagtctgtg gatcgatttc tacgtggact tcgatcgatt ctggtgtagc
     2281 gcctgattca atcgctttat tcatcgcttc atttttcaac gatcgtatat cttctttact
     2341 tggcgaaggg atgatacgtt ctacaacatc tcggaccatt gcaagtgcta caccaataga
     2401 agaaatcact tcggcgtttt caggaatcga atatttcaca cccattttat tactaaagta
     2461 agtaatcaat gaggcagcgc cgccccctac acctacaaga gaaatctgat ctttctccaa
     2521 gtgatacttg tctgccaatt ctaaaatcac cggttcaatc ttagcatagg atttttccat
     2581 gatctgttct gcgatgtctt caaccgtcgt atgacaataa tcagctaatg cttggatggc
     2641 ttttcgtgca gatgggacat ttccataaga aaaatgttct tcttgcacta atccaaggac
     2701 attggctgca catgtatttg taatagtcac ttcttctccg tcttccatca ctaccttgac
     2761 gtaatcagca gggtctcctt ctttaggaga gaaaaattca accttaggac ctttgatttt
     2821 ttctgtctcc gtaaatactg cataatctag accggcaata tgtgcagaac gtggaccgac
     2881 atcgatgatt cctgactgat tagcccgtac catcgaaccg cctgccacac ctagaacccg
     2941 aacatctaat gaagagatat atgttgggtg gccacctaca attgaataat cgattgctgg
     3001 acgtccattt ttaatgaccc cgatattggt tgttgttccg cctacttcaa aataaacacc
     3061 attagacgct ctcaagtaca tcaaagagcc catgactgat gctgctggac cggaaagcat
     3121 ggttaaaaca ggtcgttttt tcatttcatt gatttccatt actccgccgt ctccgcgcat
     3181 aatcatcaaa gaaacattga cccctgcttc tcgtacggaa tcttctgtag aggttgccgt
     3241 atctagcatt tttggtaaaa tagatgcatt gattgctgct gtccgagttc ttcttgtcaa
     3301 tccatataat tttgtgatgt cagaagccat cgttgtaggg atcgatttta ccgatgctgc
     3361 ttcataaaca actcgttctg gttccccatt atctactcca aatgccatag aagaaaccaa
     3421 gacctccgct ctttctcttt ctaaagaaga aatcgtttct gaaacacggt ctacatttaa
     3481 gtgtttgaca ggtaagaatg cattgacaat ctctattttt ttcttattcc ctaaatcgat
     3541 atcgttcaat ctagtttgtc gttttgctaa aaaaccttct aatccacctt ttgccatccc
     3601 gattacgccg acttttgcta cgtctccttc aatcagtgca ttagtcgctt gtgtggtaga
     3661 atgtgcaaca aacacaacat cttctggact gatattattt tccagcaaac aattttgaaa
     3721 agactgaacg acacctgcgg ctactccacg gacatcatcg tgggttgttt tcactgaaga
     3781 ttttccgatg atttcgtgcg tcgcattatc gattgcgact gctttcgtat gtgtaccgcc
     3841 aacatcaatt cccattcgca caaaacgttt cattttcttt tcctccattc atctaatcgt
     3901 ttttatcgac gttttgttta ctaggcgttc ccgtacaata agaacgtgat caggcataag
     3961 atcgctccga tgatccaacc agttgggatg gatttcttca tatattctcg agaagttact
     4021 tttgtataac ctaatcccca agcaacccaa gattgtgtaa tatctaaatg ctgaggagca
     4081 atcgttgtga ttgcaaataa cgggaacaaa aaggctggcg acatcgttgg attaacagct
     4141 aatacgacag ctaaaatagc tgagcctgaa ccgactagat tcattggacc gcggaaaaat
     4201 cctagtggtg tcaatatagc aaatacgatt gccaagacta atggtgattg cggtatccaa
     4261 tctccaaaaa tagctgaaaa ataaggagaa gcatacgcag ctgaattatt gaacatcgct
     4321 aaggtcatca agaaaccaac cattggcgca acatctactg cgccatctgt aaataatttc
     4381 gcaaactgac gacagatttc tttataacct ccgcgcaatt ttccagtaaa cagcaatgcc
     4441 cagatacctg ctagaataaa acctaagatg attggaagat ctaaaagaac gacccctaaa
     4501 actggtaata atacggctaa ccatgagatc attggtgcgt cgccaccttc agctggtacg
     4561 tttgctgcca ttgcatagcg tttctttttg ttcattgata tattagcgac cgttagtacc
     4621 acaactaagc ttacaatcat cccaatcata ccaatttgga aatagtcatt ataagtatag
     4681 ctttcagccg ctggattgaa accagcataa atcgtttgat attgtttgaa attgacgata
     4741 ttagcaaaga ttccagccat aatcgagccc ataaatgaga acagtgcaac cggtactgaa
     4801 atacctaatg acatcaagat tggcaacaca ataactgcaa tagaaatcac tggtccgatt
     4861 cctgtcatcg acatgaatat aacagcggtt acaatacata gtaagctcat cgtgattctt
     4921 ggcttgtccc ctccaagttc aactaccttt cgaatcaggg tagctgcgat tccgctatct
     4981 actagaacac gcccgaaaaa ggcaccaaag aagacattta ccaagatcga tttggcgtac
     5041 tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa ttactccttg cccttccatc
     5101 gccgggtttg gtgaaaacgt gtttccgact agcgctaatc ccatccatac agtagccatg
     5161 atggcaaagc cgaccatcaa gttgtagcct tttacacagt accagaccat gaaaaagaat
     5221 gagataacta acaaaatacc aataataata tcactcatgt tattcctccg tccattttgt
     5281 ttttctgata agcaaactaa tatatcagtc aaactcattc taacttatgt agttagcgct
     5341 ttcattaaag ggtgaccata aatgttcagt tttcaattaa aaaaccttaa ttatgctatt
     5401 ttagacttat acgttattgt cctgtttcat ttgatggagg attgtaaata aaaaaagagg
     5461 aaagaacata acttttcgtt atcttctttc ctctttataa ttgtttagct tatttagcca
     5521 aagcttcttt tgcttggtca gccaatgcag taaatgctgc tgcatcgtta acagccaagt
     5581 cagctaacat tttacggttg atgtcgattt cagcaacttt caaaccgtgc attaattttg
     5641 agtagctcaa gccattcata cgagctgctg cgttgatacg agcaatccac aatttacgga
     5701 agtcgcgttt cttttgacga cgatcgcgat atgcgtagtt gtatgaattc attacttgtt
     5761 cttttgctgt tttaaataaa gtgtgttttg aaccgtagta accttttgct aattttagca
     5821 ctcttttacg acgtttacga gttactgttc cacctttaac acgtgccatg tataattcct
     5881 cctaataaaa ataaatcttc agattctgtt atttgtactt cttatttcat ttttgctagt
     5941 tgttggcgaa tacgtttgaa gtcgccacta gaaaccatgc ttgctttacg caattgacgg
     6001 cgttgttttt ttgttttgcc gtggaaacgg tgacttgtaa acgcgcggaa gcgtttcaat
     6061 ccgcctttac cagtacgttt gaaacgtttt gctgatccgc ggtgtgtttt ttgttttggc
     6121 atgactatat ttcctcctca aaatctttcg ttatccgact atcggctgtc caacagccag
     6181 ttagtcattc aaattactta ctgtcgtttt tcggtgccag cgtcaagaac atgctgcgtc
     6241 cgtccatttt cgctttttgt tccactgttg caatatccgc agtttcttca gctaagcgat
     6301 tcaagacttt ttgaccaatt tctttatggg taatggcacg gcctttgaaa cggatagaag
     6361 ctttcacttt atcgcccttt tccaagaact tacgtgcatt acgaagtttt gtattgaagt
     6421 cattcaagtc gatcgtagga cttaagcgaa cttcttttac attgatcact ttttgttttt
     6481 tacgcgcttc gcggtctttc ttttgttgtt cgaaacggta tttaccgtag tccatgattc
     6541 gcgcaactgg tggtttcgca ccaggggcaa ctaacacaag atccaagttt gcttgttctg
     6601 caatattcaa tgcttctgct tttgtcttaa ctcctaattg ctcaccatct gaaccgatca
     6661 atcgtaactc tcgtgcacga atgccgtcgt taaccatcat atcctttgct atggtcattc
     6721 acctccaaat attatgagag aaagaaaagc ttaccgaata aacggcaata aaaaaacgag
     6781 ttctcttaca gaacccgcgc gtacttcacc taatagaacc agtgaaaact atctagccca
     6841 gtgacattat caactaaggc gagaagcggg tgcttctgct tttatttctc aaccatcaca
     6901 gtatagcaaa aactaatctt tttgtcaatg tttttcatac agtttgtctt ccttttctga
     6961 aaatgattag attagcagac tttctagcag tgattcccgc ttctttgcta tactaaagaa
     7021 aaacgaggtg aacccacatg aaaaaaatcg aacctattcc accttctctt cctcttctta
     7081 caaagggaga gttttattta gaagatgagg agatcttctc tggtcttctg atcaacgggg
     7141 gtgaccaaag ctatctcgat gccaaaaata tggtattaag agagagtcat atccaaaaac
     7201 ttacgatgca aaaaacagtt ttacgtcatt ttgaatgcag taatgtcatt tttgaaaaat
     7261 gtgatttttc caatctggag tggattggtg gcagtttcca ccaagtcgtg tttcatcaat
     7321 gtaaattaac aggtacgaat tttgccgaaa gttatcttcg agactgtact ttcactgatt
     7381 gtattgcttc gatggcttcc tttagtagta caaacttgaa agctgtctcc ttcgatcatt
     7441 gtcaattaga agacagcgaa ttttatgagg tcacttggaa aaaccttttt ttgtctgaca
     7501 atcagttgac cggttcgaac tggtttcgaa cttctctgaa gggattagac tttacgacta
     7561 atttcttcac agcaatcgct ttatctcaag attctttggc gggactaatc gtcaaccaag
     7621 agcaagcact tgtgatcgcc caagcccttg gtttgcagat caaagactag atcaacgaat
     7681 ccgccgccac agtgcaataa gcatccaaat agataagacc aaactgatga tcaaggcgat
     7741 tccccatccg aaaacggaat gggtaaaggg taactggaga ttcattccga agaatcctgt
     7801 cacaatagtt ggaacggtca agatcagcga ccagaccgtc aaaaatttca tcgtgtcatt
     7861 caagttgtta ttcagcaaat tattataagt gccagagagc tggtctaaaa tctgcgaagc
     7921 taagttcgtc atttcaactg cttgtcgtgc ttcgatcagc gcatcgtcca actcttcttt
     7981 ttccttttct gacaacttgc gataaatagc caatgccttg atttgttcta aaagtactac
     8041 attttgtttt gttcctgtaa ctaagtaaac caaaccgatc tctaaatctg aaagttgcag
     8101 cagattcttg ttcgttgttt tttctcttaa tttcagatta agccgctgac gttcactatt
     8161 cacttcctcg attaaaggaa aaaaggaatc agaaatcaaa aacaatgtac gaaacaataa
     8221 actgtataca ctcatgtctg gcattcgttc caaaagcgat cgaattagtc gaatcacgta
     8281 ctcggtattc ggcgtggtaa atgtaaacaa accgtcttct tttaaaataa aagcgatagg
     8341 actagtttca taatgattat cgactttttg ctggtgcgga acatttgata caagcagtaa
     8401 cgcttcatca aaagaatcgt actctacgcg cgcccgttca tttttatcta gagaataagt
     8461 cagcatctcg tcactaatgc catattcttc ttttaagttt ttaacagcta gcacatcgtt
     8521 ttgctgtaca ttgatccaaa aattacggtc ttgattaaaa tagtgttttt cccgcatact
     8581 ctatcctcct gacatttgtt tccagtataa agaaaaaagt cgctcaaata aaggtttacc
     8641 ctttatttga gcaactgtaa atcaaatggc tactttggtt tggcgaatct ctgctaattt
     8701 ttcttcgatc attttcaaga cttcttcttt tgcttgagga tcttccacaa aattatagcg
     8761 gtctccatcg atttggattt ttggactttc tttgtattct tcataccatt gatcgtaacg
     8821 tctgttcaat tctttgtaat aatcgtatag acttggatca aaggacaatt gttcatacga
     8881 acgaccacgt ttttcgatac gctccaacat cgtatcaaaa gatacacgaa tatgaactaa
     8941 taaatccgga tgttttttat gggcagcata gggaagttct tccatcatgt tttccagcag
     9001 ttcatcatac acacgaactt ctgtttctgt tgctcttcct agatctgcat ttagatggaa
     9061 aagcagtgaa tcttcataaa tcgaacggtc caatacatta ttgtcatctt tcattgcttg
     9121 tttgatgctt gcaaaacgtt tattcaaaaa ataaatttgc agcaaaaaag catactgttc
     9181 agggtttgca taaaataagg gcaagacttc attatcatca atcgattcat aaaatgcctg
     9241 actatcaaag tgttcagcca tcatttctgt taaacttgat tttccggcac caattgtacc
     9301 tgctaaaacg atcacgctca tttacctcca gctcacttca tcactaaata ttgtgtttaa
     9361 ctccaacctt tttaaatata cccccaatat ctagtagtgt caattgtatt tttttctagt
     9421 caaagaagaa aaaaagtcaa aaaattctta agaaaaaagt gttttttctt tagaaaacgt
     9481 attcttagtt ttacttctga gatggtttta gtaaaataaa ggaggaatgt tttttgagac
     9541 ccttagtgga caaaaaaatt cgtgaagcag cgcaactcgt tttgggcgct ttattttttg
     9601 cttaaaaatc acttttacgt gagaaggagt tagattatgg caaactatgg ccaaggagaa
     9661 tcaagcggaa agatcatatt gatgggcgag cacgcggttg tttatggaga accagcgatt
     9721 gcctttcctt tctatgcaac aaaagtcacc gcattccttg aagagctgga tgcaatggac
     9781 gatcaactgg tttcttccta ctattcagga aatttagccg aagctcctca tgcattaaaa
     9841 aatatcaaaa aattattcat tcacttaaaa aaacagcatg acatccaaaa aaacttgcaa
     9901 ctgaccattg aaagcacgat tcctgctgaa cgtggaatgg gatcaagcgc tgcagtcgcc
     9961 acagcagtca ctcgtgcttt ttatgattac ttagcatttc ctttgtctcg tgaaatacta
    10021 ttagaaaatg tccagctttc ggaaaaaatc gcccacggta atcctagtgg aatcgatgca
    10081 gccgctacta gcagcttgca gccgatttat tttacaaaag ggcatccttt cgactacttt
    10141 tctttgaaca tcgatgcttt tttgattgtc gctgatacag gaatcaaagg acaaacaaga
    10201 gaagccgtca aagatgtcgc tcacctcttt gaaacccagc ctcatgaaac tggacaaatg
    10261 atccaaaaat taggatactt gacgaagcaa gcaaaacaag caatcattga aaattcacca
    10321 gaaacgttgg cacagacaat ggatgaatca caatcacttc tggaaaagct gacaatcagc
    10381 aatgattttc ttaatctatt gatccaaaca gcaaaagata ccggggcatt gggtgccaaa
    10441 ttaactggcg gtggacgcgg tggctgtatg attgcattag cacaaacaaa aacaaaagcc
    10501 caagaaatca gtcaagcact tgaagatgcg ggtgctgctg aaacttggat acaaggatta
    10561 ggagtacata cctatgttta aaggcaaagc acgcgcatat accaacattg ctctaatcaa
    10621 atattggggt aagaaaaatg aagaactcat ccttccaatg aacaacagcc tttcgttaac
    10681 attggatgca ttttatacag agacagaagt catcttttct gatagctata tggtagatga
    10741 attttatcta gatggcacct tgcaagacga aaaagcaaca aaaaaagtca gtcagtttct
    10801 tgaccttttt cgtaaagaag ccggcctctc actaaaagct tcagtaatca gtcagaattt
    10861 cgttcctacc gcagctggat tagcctcctc tgccagcggg ctagctgctt tagcaggcgc
    10921 ttgcaatact gctcttaagc ttggattaga tgatctctct ctttcaagat ttgctcgacg
    10981 cgggtctggt tcagcttgcc gaagtatttt cggtggtttc gtcgaatggg aaaaaggcca
    11041 tgacgactta agttcttacg ctaagccagt cccttccgat tctttcgaag acgatttagc
    11101 aatggttttc gttttgatca acgaccagaa aaaagaagtg tccagcagaa atgggatgcg
    11161 tcggacagtc gaaacatcca gtttttatca aggctggtta gattccgttg aaggggatct
    11221 atatcaattg aaacaagcaa tcaaaacaaa agatttccaa cttctcggag aaacgatgga
    11281 aagaaacgga ctaaaaatgc atggaacgac tttagctgcc cagcctccat tcacttactg
    11341 gtctcctgac agcttaaaag cgatggatgc ggtgcgacaa ttgagaaagc aaggcattcc
    11401 atgctacttt acgatggatg ctggccctaa tgtcaaagtt ttagtggaaa acagccattt
    11461 atctgaggta caagaaacat ttaccaaatt atttagcaaa gaacaagtaa ttactgcaca
    11521 tgctggtcca ggaattgcga ttattgaata aagggagatt tttatgattg aagtatctgc
    11581 accaggcaaa ctctatattg ccggagaata tgcagttgtt gaaacaggcc atccagcagt
    11641 tatcgctgca gtcgatcaat tcgtgacagt cactgtagaa tctgcacgaa aagtcggaag
    11701 tatccaatct gcacaatata gtgggatgcc tgtacgttgg acaagacgca acggagaatt
    11761 agttttagat attcgggaaa atccttttca ttatatcctt gctgctattc gcttgactga
    11821 aaagtatgca caagaaaaaa acattctttt atccttttat gatctgaaag taacaagtga
    11881 gttagacagt tcaaatggcc ggaaatatgg attgggttct agcggtgccg tgaccgttgc
    11941 aacggtcaaa gccttgaatg ttttttacgc gttgaattta tctcagttgg agattttcaa
    12001 gattgcagca ctagccaatt tagcagttca agataatggt tcctgcggcg acatcgctgc
    12061 tagctgttat ggtggctgga tcgctttctc aaccttcgac catccttggc tccaagaaca
    12121 agaaactcag cattctatca gtgagttact tgccctggat tggccaggtc tatccattga
    12181 gccattgatt gctcctgaag atttacgttt attgattggt tggacgggta gccctgcctc
    12241 tacttctgat ttggtcgatc aagttcaccg ttcgagagaa gataaaatgg tggcttatca
    12301 gcttttctta aaaaatagta cagaatgtgt caacgaaatg atcaaagggt ttaaagaaaa
    12361 taatgtaacg ttgatccaac agatgattcg aaaaaaccga caattactgc atgatttatc
    12421 tgcaatcact ggggtcgtca tcgaaacgcc tgctttgaac aaattgtgta atttagctga
    12481 acagtatgaa ggagccgcaa aatcttctgg tgcaggtggg ggcgattgcg gaatcgtaat
    12541 tgttgaccag aaatctggca ttcttccttt aatgagtgca tgggaaaaag cagaaatcac
    12601 tccactgccg ttacatgtct atagcgatca aagaaaggaa aaccgatgaa tcgaaaagac
    12661 gaacatgttt cactagctaa agcctttcat gataaacaaa aaaacgaatt tgacttcgta
    12721 cggatcatcc ataacccttt gccgcaaata gcagtttctg atgtcgatct aagtacacag
    12781 gcagttgggt ttacactaag cagcccattt tatatcaatg cgatgactgg cggaagcgaa
    12841 aaaacaaaaa aaatcaacca agatcttgca atcgtcgcaa gagaagcaga tttgatgatt
    12901 gccactggtt cagtcagcgc tgccttgaaa gtcccttctt tagccgatac ttatacgatt
    12961 atgcgacaag aatatcctca cgggaaaatc attgccaaca tcggtgcagg gacttctgtc
    13021 gaaagagcca aagaagctat ccgattgttt cacgcagatg ccttacagat tcatttgaat
    13081 gcacctcaag agttggttat gcctgaggga gaccgtgatt ttaccaattg gaaagtactt
    13141 attcaagaaa ctcaaacagc cattgacgtc cccctcatcg taaaagaagt cggttttggc
    13201 atgacaagag aaacgctcaa tgatctagct gctttaggag ttcatacagt tgatatcagc
    13261 ggtcgaagcg gaacaagctt cacccagatc gaaaacgccc gtcgctccaa gcgggaactg
    13321 agttatttag ctgactgggg gcaatcgaca gtctcttcat tgcttgaagc aaatgaagca
    13381 gacacttcta tggaaatcct agcctccgga ggtatccgca atgcctatga catatttaaa
    13441 gccctttgtc tcggcgcaaa tgccgtgggt acttcaggaa ccgtactgac gcatctgatg
    13501 aatcacggcg tagaagaaac tattatactg atgaagcaat ggcaagaaga gcttcgtctg
    13561 ctttatacta tggtcggcgc aacgaatacc gcggccctac accaacaatc tctgatcttt
    13621 tccggaccag tgaaggattg gtgcgaagca agagggatcg acttagtgaa gtatggaaga
    13681 cgcacagaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    13741 tacaaacggc ggaagtaaaa gcaactcctt cggaaataag ctgaaattca caaaaatttg
    13801 aaaagcaatt ttcgtgaatt ccttcttatt tcttggagtt aaatgcttct gtcccagcct
    13861 cttttttcga ataaatgatg tttaatcagc tgtccttcgg tattcgttca atccgctgat
    13921 ttcacaacct ctccactaaa cagtgtacta aagctctttt aaggttgttt tccgatatag
    13981 gcaagtatgc ctccgtccac atacaagaca tggccgttaa cgaaatcaga tgcgctagaa
    14041 gccaagaaga cagatggtcc agccaaatcg attggctcgc cccagcgtgc tgctggcgta
    14101 cgtccaacga taaattgatc gaatggatga cgttcaccgt tttcttgtgt ttcgcgtaat
    14161 ggagcagttt gcggtgtagc aatataacct ggtccaattc cattacattg gatattgtat
    14221 tgaccgtatt cagaagcgat gtttttcgtc agcattttta atccgccttt tgccgcagca
    14281 taagcactga ccgtttcgcg tcctaattca ctcatcatgg agcagatatt gatgatttta
    14341 ccgccgcctt tttcgatcat atccggaata actgcttttg aaacgatgaa tggggcattt
    14401 aaatccacgt cgattacttg gcggaagtct gccgcatcca tttcgatcat cggtgtccgt
    14461 ttgatgatcc ctgcattgtt gactagaata tcaactgaac ctacatcttc tttgatctgt
    14521 tgaaccatcg cttgaaccgc ttcttcatcg gtcacatcgc agacataacc ttttgcatcg
    14581 atgcctgctt ctttataatg cgctatacct tcatcaactg attcttgatt caagttatta
    14641 aatacgattt ttgctcctgc tgtagctaaa gagcgtgcaa tctcaaatcc aataccatag
    14701 acagcaccgg tgactaatgc tacttttcca tctaaacgaa acatatccat actaaattcc
    14761 atcattttct ctcccttatg cttattttaa ttgatccatt tgcaccatat ccatgtctgt
    14821 ataggtgata ttttctccgc acattgccca gatgaatgag taattgcttg tacctacacc
    14881 tgaatggatc gaccagcttg gcgaaataat ggcttgttca ttgtctacaa ctaaatgttt
    14941 tgtttcatct ggtttgccca tcatgtggaa cacacgtgta tcttcattgg cataatcaaa
    15001 gtagacataa gcttccatac gacgttcatg tgtatgacaa ggcatcgtat tccaagcgct
    15061 tcctggttct aaaatcgtat aacccatttg taattgacag ctttcgcata catttggatg
    15121 gatgtattgg tagattttac gttcattcag tgtcaggcct tcaccagtct ccattggttt
    15181 tacttggtcg atactgattt ttacatttgg atatttatga tgagctggaa cagaactaat
    15241 atagaattta gctgggtttt cagcatcttg agaagaaaat acaacatgtc gtgtttcttt
    15301 tccgatatag tatccgtctt gttttttcat ttcttctttt tgcccatcga tctcgatgaa
    15361 tcctggtcca ccgatattga tcacacctag ttcacgacgc tctaagaagt aatcaacacc
    15421 tagttctttg tctaatgtga tttctaattc tttttcagta ggtgtcactc caccaaagat
    15481 cattcgatcg ttgtgcgtat aagtcaaact gattttccct ggttcaaata ccttttctac
    15541 taaaaattct tttctcaatt gttccgttga ataatgacgg atatcttctg gactgtgtgt
    15601 ataacgtgtt tccatttctt gcattttctt ctactccttc tatcgaatga tttttccaga
    15661 gccgcatgct aaaaattgtg cgacttcttc ttgactaaat tggttgcagt ccccatggat
    15721 cgtatgcttc aaggcagaag ctgccgtagc aaaatcaatg atttcttgcg gagacatatt
    15781 ggataaaatc ccatggagca cgccacctgc aaaagcatct ccgccaccta ctcgatccac
    15841 gattggggtg attctatgga cttgggattc cacatattca tggttcatcc acaatgtccc
    15901 cttcaattca ttttcacttg ctgaatgaac ggtacgcttg gtcgaataaa acacctgaat
    15961 attcgggaac ttttcctgca ttttttgata ataataaatt agaggttgtt cttctgcttc
    16021 tggcgctttc tcgataccta acaaataaag agcatccatt tttccagctg agcagatatc
    16081 tactaaaggt aaaagctgat ggattacttc gctggcttct tgctgtgtcc ataattttcc
    16141 acgatagttg atatcgaagc tgatcaggca gccagctttt tttgcagctt ctatcacctg
    16201 tttcgtcatt ttttgccatt ttttagacaa agctggggtg attcctgaaa catgaaatat
    16261 atctattcct tggaataact cctctagcga ccattccaaa tgatccattt cagcaaaact
    16321 tgatccggca cgatcataga tcactgaggc ggcgcgttcg ccactaccag tttccatata
    16381 ataagtacct agtcttgatc caccagataa tacctgtttc gtatctactc cataacattg
    16441 caagtgcttt ttgactgctt ttcccaatgc gttatcaggt acttttgtag caaaaatcgc
    16501 ttcatgtccg taattagcta aagaaattgc tacgtttgct tcccctccgc cataatgggc
    16561 agcgaatcgt tcacttgaat cgatacgggt tccctgctct gtggacaatc gcaacatgat
    16621 ttctccgagt gtcactactt ttcccataag cagctacctc ttgatttctt tcatttttgc
    16681 tgcatactgt tgcgcgactt ccgttacttt gtcaaaatct cctgtagctg cgggtgctaa
    16741 aaggttccca cctactccga ctgttacgac accagcatcg aaccactctt tcatattgtc
    16801 cagactcaca ccgccagttg gcatgatatt gacttgaggc atcggcgcct tgaaggcaga
    16861 aatgatactt ggtccataag cgcttccagg aaatagtttg acgatatcta ccccgctttt
    16921 cagcgctgtt ttgatctcgg taatagtcat acatcctggt aagtaaggaa tttgatacag
    16981 attgcacatc tctgccgttt cttgatcaaa ggaagggcta acaatatact ctgcgccagc
    17041 taagatagcc aagcgagctg tgacagcgtc taatactgtg cctgcgccaa tcactatttc
    17101 tggttggtct ttgtagaatg acaaaagttc tttgattact tgatctgctt gagggactgt
    17161 aaaagtcaac tcaataccag ttagaccacc tttgatgatt gcgtggcaag cattcaaagc
    17221 ttcttctttg ctttttccgc gtaccaccgc aatcactccg gcgtttttca atcgagataa
    17281 aatatcaatt tttttcatct tctccaccta tttcctattt agaaatttta ttttataact
    17341 tggaaatcta tacccatatt atacggcaga aaagaaaaat tgcaaacgct tttttcatta
    17401 tttttgaatt ctttgctcga tttcgctttt tgtttgcttg agggcactga ttatttgttc
    17461 ttttgtctca tctgttaaac gatatttcgg catacttaca ctaaatgcac caatgatttg
    17521 ttcgtttttc acgattgagg ctcctataca aaaaatatca cgttcgattt cttcatcgtc
    17581 aaatgcgatg tgggtttctt ttaccgtttt gatttccttt ttcaaccgta atgcattggt
    17641 gatcgtatat tctgtgtaag ggatcaacgg atgcttttca agataatcag tgtactgctc
    17701 ttcactgaat tctgctaata cggctttgcc catcgcagaa ttgtataatg gtcgggtgat
    17761 tccgattttt gaagacatgc ggatcgtttg atgttttgga tctagtttgt tcacgtacaa
    17821 gatctcattg ttgtccaata cccctaagtg gatagtctca tcgatctttt gctgcagctg
    17881 ttctaaaaac ggtaaagtca actctaccaa atcgatttgt tcaatgtttt tattcgcgta
    17941 gcgaataagc tttgcaccta aacgatagtt tttctctgaa tttttattta cgtagccgat
    18001 catcaacaaa gtatctagta ttttcaagac tgtagaagag gtcattccca caccctttgc
    18061 gatcgtctgc aaagagacat cttggttttc cgctaaatag tctagtattt tagaggcttt
    18121 gatcaataca gttccataag gctttttatt ttccatataa tattctccta tttctttata
    18181 agaaaaatta tagcggaaat accatcaatt taaaagagat atccagatat aaactaccat
    18241 ttctctttac aggcaattta atgttgaaga tccgcttcgt ttttacatca aactttgata
    18301 taaagcaatg atttcttcta ctgtcgtttc tcttggattt cctggtgtgc atacatctcg
    18361 caaagcatct tctgcaattg ctggaatatc tgcttctttt acacctaatt cagaaatggt
    18421 ttctgggatt ccaaccgctt tgctcaaccg atcgatctca ccacaagccg cttctcttac
    18481 atcttctaaa gacatttcag ctgctccttt aatccctaga acaagagcga tctcacggta
    18541 cttttctcca gtatattctt tgttatattt catgacagtt ggcaaaagtg ctgcacacgc
    18601 tacgccatga ggaatgttgt accatgcact taacgggtga gccatcccgt gtaccagtcc
    18661 taaaccgaca tttgagaagc ccattcccgc aatatactgt cctaatgcca ttttttctcg
    18721 cccattttga tcgccatcta cagaagcttc caaagaatgg ccgatgattt cgattgcttt
    18781 taaatgcagc atatcagtca tttcccaagc gccttttgtg ataaaccctt caatagcatg
    18841 cgtcatagca tccatacctg ttgatgcagc caattttttc ggcattccca tcatcatatc
    18901 gctgtcgatg aacgcgacga ttgggatatc atgaggatca acacaaacaa attttcggtg
    18961 atttacttca tctgtgatga cgtagttgat cgttacttct gctgctgttc cagaagtcgt
    19021 tggtatagct aatataggta aacatggatt tttcgtatca gcaacacctt ctaaactaat
    19081 gacgtctgaa aactctggat tcgtcaaaat aatcccaatg gcttttgccg tatcgattgg
    19141 cgaacctcca ccgattgcga tcaaacagtc tgcctctgcc tctttaacaa aagccactcc
    19201 atcttttaca ttttggatcg ttggattagg tacgatcccg tcgtataaag cataagcgat
    19261 tgctgcttta tctagaagtt ctgttacttt tgttgctata ccgtgctcga tcaatccttt
    19321 gtcagtaatg acagctgctt tgccgaatcc tcgctgctga aattcaggga cgatcgactc
    19381 aatcgctcct tttccaaaat acgatgtttc atttaaaatc attcgattac tcattttcta
    19441 ttcctcctaa tttgtattat ttttcacaat aaaaacgaaa gaaccccaag caaccggttg
    19501 cccagttgct tgttgctaca gttcaaagac tggcatcaat tcccaactga ctggagaggc
    19561 atctggattt gtctccatga tatcttccat gtacttccac catttttgat tgatagatgt
    19621 atttgccatt ttttcccata acttttcatc ttcgatcact aagtaaccaa ataacaaact
    19681 tgtactcgga tcaagataga tcgaatagtc gatgaccccg tgttcacgaa gtgctgtacg
    19741 catttctggc caaagttcat catgtctttt tttgtattct tcttgttttt ctgggtaaac
    19801 tttcatgcaa aaagcttttt tcaccatgtc agacacctgc tttcgtatga acttcctcca
    19861 aataacctgc ttttggagtg acaccaaacg catctgctaa atgccacagg ttttcgtccg
    19921 tgattgtttg caagattggt ccttgcgctt tgacgtacgt atagacttct gctgcctttt
    19981 ctgctgtctc gatcaaacca aagacttcat ccatgtcttt tcctgacccg tagattccat
    20041 gttgtggcca taaaaccaat cgagtttctt tcattttttc agctgttgct tccccaatct
    20101 catttgttcc cggaaccatc cacggaatga tggaaattcc ttctggaaaa acaactaagc
    20161 attccgtaca catttgccat agcgtacgac taaaggaacg ttcgtctaat tcatgcgtaa
    20221 aactcatcgc taatagatga ctagcatggc aatgcataac gatccgatta tctgggtcta
    20281 cttctaaacg ggcgatatga ctcatgaaat gacttggcaa ctcgcttgta ggaaggcatt
    20341 cgttttccag cccccacaaa agttccagtg ttttgccatc ttcagcaact cgaacgattc
    20401 caagattctc ggcaggttcg ctcgctacat ttttaaaata tcttcctgaa ccagttacaa
    20461 taaaatagca tcctgctaaa cggctggcat caaaaatcat cgagatgcgg cggatcactt
    20521 tttcaggatc caagaacggt gtgatctctt cttcttttag taaataagaa atattcccgc
    20581 catttctttc atcccatccc aagcgataaa gattcgacgt cgtttcgatc atttctttca
    20641 cataaggcgc ttgtaaaata tctatttttt tcaatatagc cacactccta ttttcttaat
    20701 tccaacacgt ctttttgata ttcctggact tcttccagcc aatccaaacc aactggaact
    20761 tgatttttgt aacagaaata attccataca tcggcatacg ggaaatcttt caattcttcc
    20821 gtataagcca ttcttgaagt aaagtcaaaa gccagctcca tttctttcaa tcgctcaact
    20881 ggttccagca tagctttcag cagtgctttt tgtgtatttc tcgtaccgat gacccaagca
    20941 gcgatacggt tgatcgttgc atcaaagaaa tccagaccga tatttgtttt ttccaacaat
    21001 tcgttacgga ctaattcttt agcgatttcc tgcaattcat catccatcaa aaccacatgg
    21061 tctgaatccc aacgcactgg acgggaaaca tgcaacatga ttcctttgct gaataaagct
    21121 aatgaagata atttattgga aatcacttct gttggatgaa aatgtccagc atctagacaa
    21181 acaagtttgt cacgagtcaa cccgtaaccc atataaaatt catgggaacc gactgtataa
    21241 gcttcagcgc ctaaaccaaa tagtttgctt tctactgcat cttgtacata ccgttcatct
    21301 aactcttctg taaaaatttc atcgagtgat tccattaaac gttcccgtgg ggtaaaacga
    21361 tcaatcggat tatctttcat cccatcaggt acccaaaagt tattgatact tgtttgccca
    21421 gtttctttcc caaagtactc cgcgattttg cggcttcttt tcccgtgttc gatccagtat
    21481 tcacgaactt ctttatctgg atgagccaat gtaaagccgt ctttcatcat tggatgcgaa
    21541 aagaaagtcg gattgaagtc aagaccgatt tcttcttttt ttgcccactc tacccatttt
    21601 tcaaaatgtt tcggctcaat ttcatttaga tcgacagatt cttctgtatc caaatagata
    21661 gcatgaaggt tcaatttgtg tttgcccggt atcaacgaat aagccttctc cagatcttgt
    21721 ctaagctctt caggtgttcg tgcggctcct ggaaaattcc ctgtcgccat gattccaccg
    21781 gtcaattcgc catccggtgt caagaaacct ttgacatcat ctccttgcca gcaatgcata
    21841 gaaattttga tgtcctgtat cttttctagt acagcatctg tatctacacc gatagttgca
    21901 tatttttctt ttgcttgttc gtaatttctt tcaatcgtgt tcatacgatt actcctttga
    21961 ttttattcac gtaccattcc atcgtgtttt gcttattttc atctgggtaa aaagtttcac
    22021 atggaaacga ctgtttgatt acatttcttg cttgttctat attcgaaaac tgacctgtac
    22081 tgagcatttg catggcaaca ttcccgattg ctgtggcttc actaggtcct gcttctacaa
    22141 tgattccggc ataatctgct gtcaattgat tcagtagacg attattcgat ccgcccccta
    22201 cgatgtgcag cttttccaat ttcgttccag ttaagtgttc tactttgatc agctcttgtg
    22261 cgtaacaaag agcaagatta tcatacacac aacgagcgat agctcccgat gagacaggga
    22321 ctggctgctc tgtttcaaaa cagtattgct gaatctcttc tatcatattt gggggattca
    22381 aaaatcgagg gtcattgatt tccatcactt gctgcagcgg tttttctttt gcagcaagct
    22441 ctgccaattc cgcataactg tactcgtact cttgcattcg ggcaacttcc tggatcaacc
    22501 acatccccat gatgtttttc aaaaaacgca ccgtgttatg ggcaccccat tcattcgtat
    22561 aattttcaga aaaagcattg cgagaaacat tggcgatctt tgtttcaatc cctaaaagcg
    22621 accatgtccc actgctgata taaccccaac gttctccttg acctggcgtt cctaatacag
    22681 ctgacgccgt atcatgactt gctactgtga taaaggtcac atctggcaaa tcatattttg
    22741 aaaattgttc tttttgcaga tctcctagta tcgttcccgg ctctgtcaat ggtggaaata
    22801 attccttcga gacaccgatt ttttcaagaa gttctatttc ccattctttt gtccctgcat
    22861 tcagcagctg catagtagat gcattagttt tctccgtcac catgttacca gtaaagacat
    22921 accctaaata atcaggtatc agtaacaatt tatgtgcttt tgccaaattt tctttttctt
    22981 cagcaaacaa ttggaacaag gtattgaaag gctggatctg aatccctgtc tgctcataca
    23041 acttatctag agaatagatt tcagaaaact gttctactgc tccattggta cgcttgtccc
    23101 gataagcaat cggatcattt atccgatccc cttgttcatc aatcagacag taatcgactg
    23161 cccatgtatc gatccccgca aaaaggcgat caatccctgt ctgctttaat ttttcaaatc
    23221 caatcaaaat ctctttgata agataatcga tcttccagcg atcgtgaccg tctttgttcc
    23281 gaaagccgtt tttgaaccgg tgtacttctt ccaagatcaa agaacctttt gcatccctcg
    23341 tacttttcat tagtcgtccg cttgatgcac cgatatcgat ggcaacaaat gttggattca
    23401 tttctccacc tcttttactt tattgttgat tagcacttta ttaataaagc gttatcattt
    23461 attgacaatg ctatcataat acagaatctt atattttgct tcacactatt ttggtatata
    23521 attgcattat cttgacaagt tgggagtgtt tacatggatt actgggaaaa agccaaccac
    23581 tcatggtcgg aagactcaaa aagagtgatc ttgacgccta cacaaagaag caggaattta
    23641 tttttttata tccaagaaat cggttacttc aaagcaccaa tcccttacta tactgaacga
    23701 gcacatttac cttcttactt gataaagttt actttgtctg gcaaaggttc gcttacatat
    23761 aatgataatc agcaacagct aaaagctgga gacttatttt ttattgactg ccaagaatat
    23821 cagcgttatc aaacgatcag cgaagaacca tgggaaatgg attggatcca tttgaatggt
    23881 cagaacgtcc aatcatttta tcaggagttc acaaaagacg gccataacac cttccattca
    23941 acaggagatc catcagaaaa caagatccac catttgatca cagaattgat ccacatgcaa
    24001 caaggcacga atgcccgtac cgatttccag tcctctgtag ctatccacga gttgctgaat
    24061 gaactgattt tgcaaaagta tcagctagat ttcgaagaag aggatattcc cgcctacgtt
    24121 tacaaactga agcaatactt agacgaacat ttcaaagaaa cgattacact tgatcaatta
    24181 gaacaacaat tcttattgaa taaatatcag ctgagcaaag atttttccaa atttatcggt
    24241 tctccaccaa tcgattattt gatcagcaaa aaactttctt acgctaaaga ccttcttcgg
    24301 tatacagaat ttacggttcg aatgatttcc gaagaaatag ggattgagaa ttttgcttac
    24361 tttagccgcc tgttcaaagc aaaaacagga ctatcgccga gagactttcg taaattcagc
    24421 tgattcatat aatatgtacg tcattttttc aaaatatttt gaaatatata gataaaaata
    24481 aaataaccgc agtttctgct tctatgtaaa caacagcagc tactgcggtt attttttagt
    24541 gatcattctc attgttcatc ttcaaaatgt tcataatgaa ccaactgttt ctgtgtatca
    24601 cgtttcaaaa attctttagg cgttatgccg aactctctct tgaatgcttt cagaaaatgc
    24661 ggataagaag aaaaaccact tttactatat acagcactag agctgcggta ttctcgcaac
    24721 aagtgcttgg catagatcag ctttttcttc attacatact gatgaaacgt aaatccagtg
    24781 tactttttaa attctcgtgt gatatggtat ttgctgacaa aaaactgctt ttccatttcc
    24841 tctaaagaaa gcggatgaga taaatttttt gagatatatt gtaacatttg ttccacacgc
    24901 tcattaggaa ctacagcatt ctgttcttgc gcactctctt ctttttgtac aacttgattg
    24961 agaaaaatca agtattctac cagagcttgt tcatatttta tgtcttcacc gtaaattttt
    25021 ttatcaggag gatggtcgat aaatgataat ttttgcttca atacttcctg ttctatttgc
    25081 aatacacgac tcctcttctc tcccattggt tgaaaacaag ccgatagatt agaccatggc
    25141 gtggaacgac tttctagaaa agaaggggtg acaaagatat agacacgctc gaatacatct
    25201 gaagattggc ggacgatacg atgcaaatca ttggaatgga tgagcagcac acttcctgct
    25261 ttgatcgtaa attgtcgtcc gttcaaataa aaagtcgctt ctccctttaa tgtagcatgg
    25321 atctcataga aatcatgatg atgatagatc gttttatttt ggctgacgac atctttgaca
    25381 tgatagattt catactgatc agaaaccatc gtattgactt gttcaccgct gattttcttt
    25441 tggagcatgt gccgcacctc cttttgttca cttatgcaca attttattat accacgtttt
    25501 ttgtaatcaa aaagagctgc ttttatatac tcttataaaa aagaagagac gtggcatctt
    25561 ctagccccgc cccctccatt ctaaatatta gttaaccaac gtcttcttta cattattcaa
    25621 gaagacttct tccataacga atcccatcat cttttgtttc ttcaagaacc acatacaggt
    25681 acggacgatt cttcatgatg atttctttga tttttgtgta ctcgatcaca ccgtctccca
    25741 aatttaccgg cacgattttt tcctgttcga cacgaaaatc tttcaaatga accgcacata
    25801 tcttttcacc aaaacgttca aaagcttctt ctaccaattg gacttgttct tgataatcac
    25861 tacttgtgat caaattggtc ggatctaaaa tgattcctag atagtccgaa tctactgctt
    25921 ggatcagctg ctctaccctt gctaaagaat acaccggatg attcaaacct ggttctatcc
    25981 ccaccatcat ttggtatttt tctccagctt ttaccaagcg acggataacg gacacagctt
    26041 cagcaaatgc ttcatctgtg aaattttctt ccgtatattg gatttccggc aaaacacatc
    26101 cagtttccga agcaaccatc gacgcaccaa aggaagaagc atagcgaaga tactgctcga
    26161 actttgtcag caactcttgt cgtatcgtta aatctggatg aatcatattg atatagcaac
    26221 ttaaaattcc tacggtcact tcttgctttt ctaaaactcg tttgacataa tttcccatcc
    26281 cagaattgat ctcatttgcg ccagaaggta tttcaggaaa cgaaagacct aaggcgagct
    26341 gcagtgtatg aatcccttgt tcttttgttt tttctgcaag atcttctatc gtttggactt
    26401 cggaaagatc atgtcctcta attgtaatgt tcaacataac tattctccta actcagcata
    26461 ttgggtagcc acatgacgat ttgcgggaag taagtaatca aaaacaagac gactaagata
    26521 accccataaa aaggaagaag cggtttcagc attgcctctg cttttacgcc tcctacactt
    26581 gcaccgacat atagtcctgt acctactggc ggcgtgatgg agcctacaca cagattcatg
    26641 attgaaaaca acccgtaatg tacaggatca acccctacac ttgtgacgat tggtaaaaat
    26701 attggtgtaa aaatcaagat agcaggcgcc acatccataa acatacccac cagtaataac
    26761 acaagattga ctaataacag aataataaat ttattatccg ttacgcccat aatcaacgaa
    26821 ctaattgcct gaggaatacc ggtaaacgcc atggcgaaag acatcatgga agaagtagcc
    26881 aataagaaca tgatcgtccc tgacattgct actgattgaa acaacatatt aggtatatct
    26941 tttagtttta ccgttttata gtaaaacata gcaatcaaca gggagtaaat gacagctatc
    27001 gctgaagctt ctactgctgt aaatatcccc gtcaagatac caccgataat gatgacaatc
    27061 aatagaaggc taggaatcgc ctcttttatt atttttccag catgagccat ctgtcctttt
    27121 tcgacaacag ggtatttatt ctttttagca acgatgaatg ctactagcat gattcctagc
    27181 gcccacaaag cgcctacgag ataacctccc ataaacaaag cagagatcga agcaccacca
    27241 gcaacaagtg aatacatgat aaaagcagta cttggaggaa tgaccatacc tgtcggagct
    27301 gacgcgatat tgaccgctgt cgcaaacttc cggtcatatc cttcttccac ttgctgaggg
    27361 atcaacactc cgccaatcgc ggtagaagcg gcaatggccg agctagagat ggaaccaaat
    27421 aaagcattcc ctaacacatt cgtatgagcc aaagcccccg gcacgcgccc gacgaacaac
    27481 atcgcaaaat ccactagttt cttagctatc cctccattat tcatgataat gcccgagaga
    27541 acgaaaaacg gaatagcgat cagcgaaaaa ctattgatac ttcccaccat tttttgagct
    27601 gaactaaaag cagaaacatc aaacggtata actaacaata aagtcatcat cgatgagaat
    27661 gcaatactta tagcaatcgg catccctaat acaagcaaca caacaaatac aaaaaataac
    27721 actaatccgg cttctattgc cattttattc ctccattttt atattttttc ttgaattgct
    27781 ggcttgttgc ttaatgtatc gattagattg atcaggctat agataatgat gagtaccccc
    27841 gatacaggta ctgacagata aacatatccc ataggaatac ttaaagaagg cgagatctgc
    27901 cccattgtca atgtgacagc attccatccg ccatacacca tgatcatcgc agcaaatacc
    27961 agaaataaac tttgaacaac aacttctaaa attttacttt ttttgccagt taatttttca
    28021 cttaaaaccg ttacggctaa atgtgctttt tgtcccacaa catacgcgga agacaacatg
    28081 gcaaaccaga caagaccgaa tcgaacaagc tcttccgtga ccgtatttgg atttcctaaa
    28141 accgttctgg aaaatacttg ccataaaaca acagcgacca tcccaacaag caaaacagct
    28201 cccaggatct ctaatacctt gtttaaaatc attttcatca cagctacgct ccttgctctt
    28261 ggatcttttg ataaaggtct ttgtaggtag gatcgttttt gaatatatca tgaagtgggg
    28321 caactgcctc tcgaaacggt gtattatcta catcattgaa ttggactcca tgttcttctt
    28381 gtgctaaagc tttttcttct tcaattgctt ttgcaaaagc ttcaatttca aaaaccgttg
    28441 attctttcgc tgcttcttca atggcttttt cctgctcatc agttagctgt tctttgatgg
    28501 catcattcat gatgatgata tctggcacac gtgtatgctc atcataggaa tagtatttag
    28561 ccacaccacc atgtccagct gtatacaaga caaattcatt gttttctgat ccgttgatca
    28621 aattggattg aagagacgtg tacacttcat cacttcccat aggtacgggc gaaccgccta
    28681 aaagctctac cattttgata gcggtttcgc tttgcatcac acggatcttc tttcccttca
    28741 aatcttccgg cgtatggatc ggaccgtcta ccatataaaa gctgcgctga ccagaatcat
    28801 aataggtcaa tccggtaaat cctaaatttt tggtcgattg gtagattggc tccattattt
    28861 cttcattatc catcacctta aagaaatgtt cttcatcatt gaatagataa ggaactgaaa
    28921 aaatcgaata atctttagag aaactttcca acgctgaggc acttactttt gtgaaatcta
    28981 ctgctcctga ctgtgtcaat tcgatcagtt cacgttctcc acccagttgt ccatctggga
    29041 aatactggat ctgtacggaa ccatctgttt tttcttttac taaacgccca aattcattca
    29101 ttgcatcaat tactggctgg ctgttgcttg cataagcaaa gcgcaaggta actatctcat
    29161 ttacatctgc atctgcgaca gttgccacac tagaattatt tccgcagccg cttagaccta
    29221 gcagacctag gctaagcgaa cttgctgcaa acagcagtga tgtttttttc atatgggttt
    29281 cctttctttt aacaataaga gacgagcaat tctgtcaaac aaagcaccgt taacgactgt
    29341 ccataaggca ttgctgtgat tccgatattc cgataaaaat ctaaactgtc ccccattcct
    29401 gtcccgattg aaacgttttg aacttctcct ttttcatcga tttgttcaag caatccttgg
    29461 atagcacgat aagcgacttc tttatattct tgtggtaaat aacgtttatg gaccgctttc
    29521 aaaataccgt aagcaaatcc tgcagttgca gaagattcta cgtaagaaga aggatcatcc
    29581 aacaacgtat gccataaacc gctttcagac tgataagttt ttaatgcacg cacttgtgct
    29641 tcaagtgttt cgatcaataa tttgcgcaat gcatcttctt tcgataattc taagatttca
    29701 atgatctctg gaatagcaat cgtcaaccag cagtttccac gagcccaaaa tgcttcggca
    29761 tagttatgat ttccttcaaa cgtccagccg tgataccaca atcctgttcg tttatccatc
    29821 aagtatttga tatggatcat aaactggtaa cgagcttctt ctaaatattc tgggcgattc
    29881 aacagtttcc cgatttttgc caaaggaagc actgtcatca tcaatgtatc gtcccacaac
    29941 tgattcttat tttccggtcc atatgtggca tgctgtaacc cgtcttcatt tgtccgaggc
    30001 atgtcattca tgacccattc tgcccattgt tctaaataag gcaaatagcg tgaatctttc
    30061 gtgtcttcat acaagtaagc catagtcaat agcggtgcca ttgtattgac gtttttgggc
    30121 ggagcgcctt cttccatacg atcctcaaac cattcattga ccacttgata agcgcgagga
    30181 ttttttgtca tctggtaatt tttataaata ccatatagac cgacaccttg tggccaattc
    30241 cacactgtcc agcttttgtc atctactttc agtccatcaa agtccaacaa aaattcacca
    30301 ttatcgtctt tgatcgttgt caggttttca acgagcaggt caattttttc tttcactaat
    30361 tctttaggta caaaaatctc ttctttaata atcatcttta cgccaccttt ttaaacgctt
    30421 tcattttata tccttaatat agcagtaata ccgtccacta taaatatcgc tttttgaaca
    30481 aaaactgtct aaaattgacc taaatgttaa ataacaaaag taactttcag ttacttggaa
    30541 attaaaaaaa gaacttggag aaacaacgtt ttccaagttc tttttcatct attttcttat
    30601 cttttttcta cgctttcttt tgcttatccg ttgtaagcat caatgatgat ctgtttcaat
    30661 tcagagatca atggttcttt cggattagct gtcgtacatt ggtcttcgta agctaattca
    30721 gccatacggt ctacggttgt atctagtgtt tcttgtgtga ctccttgagc ttttagactc
    30781 atgttgatgc cgacagattt tcctaattca taaacagccg ttgctaaagc ttcgactaat
    30841 tcctctgttg tttcgccttt cagacctaag aattttgcga tatctgcgta atctgtatct
    30901 gcacggaaat agtcatattt agggaacatc gcatgttttt gtggatcttt tgcattatag
    30961 cggataatat gcggtaaaag aatcgcattt gtccgtccat gagggatccc gtattccccg
    31021 ccgattttat gtgcgactga gtgacaaata cctaggaatg cgttagcaaa cgccatacca
    31081 gccattgtag aagcgttgtg cattttttca cgtgattcca tatcaggtgt ttttactgat
    31141 ttttctaaat agtcaaagac taacttgatt gcttgtaggc tcaatccacg tgtataatct
    31201 gaagccatga ctgaaacata ggattcgatt gcatgtgtta acacatccat cccggtatct
    31261 gctgtgacag atgctggaac tgacatgacg aattgaggat cgacgattgc gacatctggc
    31321 gtcaatgcat aatctgctaa cggatatttc acatgtgttt cactgtcagt gatgaccgca
    31381 aatggcgtta cttcagaacc agtccctgat gttgtcggga tacatacgaa ttgtgttttt
    31441 tctggtttgt cgatcttata tgtccgttta cggatatcca agaatttttg ttttgcacca
    31501 aagaaagatg tgtctgggtg ttcatagaac atccacattc ccttagctgc gtccattgca
    31561 gaaccgcctc caagagcgat tactgtatct ggcttgaagt ctgccatcag tttcgttcct
    31621 gcgtatacgg tatttgtcga tgggttcggt tcaacatctg agaagacttc gatcttcaca
    31681 tcatttttac gtcgtgccaa tacttcacga actgtatcgc agtaaccgaa ttggaccata
    31741 ccagggtcac aaacgatcat gacacgttca acgttttcca ttttctctaa gtacaataga
    31801 gagttctttt caaagaagat ttttggcggt aatttgaacc attgcatgtt atttctccgt
    31861 ttcgctacag ttttgacatt gattaagttt acagcagaga cgttacgaga aacagagttc
    31921 ttaccataag aaccgcatcc caaagtcaat gacggaatca tttcgttata gatatcaccg
    31981 atgccgcctt ctgcagatgg tgtattcact aaaatacgac aagctttcat tcgaagaccg
    32041 aatttgacat gcaactcttc gttttctgaa tggataactg ctgtatgtcc taatcctcca
    32101 agattcaaca taccttcaca caaatcaaac gcatggtccg tgttgttagc tttgatcatg
    32161 gcaagaactg gagaaagttt ttctcttgat agcggataat ctgggcctac gccttccaat
    32221 tcagcaatca gcattttcgt tccttttggt acactgattc ctgccaattt tgcgatttcc
    32281 atcgcagaat gtccaacgat tgatggattg accgcatatt ttccttcgtt catcactgca
    32341 tcttctaatt tttgcaattc atctggtttt acgatataaa cttggtgtgc ttggaattct
    32401 gcttttactg ctgcatagat ttctttatcc acgatcacag cttgttcaga agcacaaatc
    32461 attccattat caaatgtttt cgaaacgatc aaatcgttca ctgcacgttt aatttttgct
    32521 gttttttcaa tataagctgg tacgttacct ggacctacac ctaaagctgg ttttcctgta
    32581 gagtaagcag acttgaccat tccagctcca cctgtcgcta agacgatcgc gatacctgga
    32641 tggttcatta gcatagaagt cgcttcgatc gaaggatgtt cgatccattg gatacaattt
    32701 tcaggagctc cggcagcgat agcagcatca cgtaccactc gtgctgcttc tgcagaagat
    32761 ttttgggcac ttggatggaa agcaaaaacg attggatttc ttgttttcaa cgcgatcatt
    32821 gatttgaaaa tcgttgtgga agtcggattc gttgttggag ttactccgca aaccacaccg
    32881 actggttcag caatttcaat caaccctttt tggacatctt cattgatcac accaactgtt
    32941 ttatcatgtt tgatattatg ccaaatatat tcggacgcat acatattttt gattgcttta
    33001 tcttcgtaaa caccgcggcc tgtttcctct acagccatct tcgctaatgg catatgttga
    33061 tccaaagctg ccattgccat ttcatgcacg atatggtcta ctttttcttg atcaaagctt
    33121 tccatttctt tcaatgctgc atttgctttt tctgccaaat catcaatcat tgcttgtacg
    33181 tcaattgtct tcgttttttc tttttctatt actttagcca tcattatcct cctaatgggt
    33241 catggttatt tgttaaataa ttcacatgta tatcatacaa tatttatttt tctatgtaaa
    33301 cacttttttg tgattttttt cacagttatt tttaaatata aaattgcttt atttacaggt
    33361 gtttgtatag aaaaatatat aaagttattt attattattt atatgacaca cccgattatt
    33421 caatattgag caatatatcc catgctctta tctctttctt ttttgaataa aaaatgaggt
    33481 aggacatctt tctgccctac ctcgtcttta aataagcttt gatacttatt tttcttcctt
    33541 ctcttctgtc tcttccagcg tttctgttgt ttcagttttt acttcaactg tctcagctgg
    33601 ttcttctgtt gtttcaacag tagtcgttgc atcatttgtt acgacagtcc ctggtttgac
    33661 cgttttgatt gctgcacgat cgtactcaag gaagatccct tcgcagtcta aaacaactgt
    33721 ccgtttgtca ttgtcgattt ctgaaacaac tccatgtaat ccgccgatcg ttacgactga
    33781 atcacctggt ttcatgctat ctaacaagtt ttgacgttct tgctgttgtt tcttttgcga
    33841 acgtgacata aaaaaccaca ttgctaatag cgcgataaac atcacaagca ttggtaatgt
    33901 tcccattttt ttcacctcac taattctgta tctacaaaaa acactttagc agaaaaatgc
    33961 tgataacact agttttttac ttaattttta aaagattttc ctttagaagc tttttgcgtt
    34021 ttctttattg aacccgtatt cttcaaaaaa ggcttctcta aattcaagca aattatcatc
    34081 catgattgct tgacgtactt gtttcattag attcaacaag aagtataaat tgtggtaaga
    34141 agtcaaacga atcccaaatg tttcatcaca tttgatcaaa tggcggatat aggcacgtgt
    34201 atagttttta catgtataac aatcacattt ctcatcaatt ggacggaaat catgggcata
    34261 tttcgcattt ttcactacta gacggccttg agaagtcata catgtgccat ttcgagcgat
    34321 tcgagtaggc aagacacagt caaacatgtc tactccacgg ataacaccat cgatcaacga
    34381 atcagcagca ccgacaccca tcaagtaacg tggtttgttt tcggggatca acggtgtagt
    34441 gaattctaat acacgattca tttctgattt aggttccccg acagataacc cgccgattga
    34501 gtaaccaggg aaatccatcc cgactagatc tttcgcgctt tgacgacgaa gatcttcaaa
    34561 ccctgctcct tggatgattc caaataatcc ttgacggtct ggattagcat gagctttcaa
    34621 tcctcgttct gcccatcgag acgtacgttc tactgatttt ttcacgtagt cgtaactttc
    34681 atcgaatggt ggacattcgt caaaactcat catgatatca gagcccaatt tattttggat
    34741 gttgattgct ttttctggtg ataaaaacat acgcgaaccg ttcaagtgat ttttgaaatg
    34801 cacgccttct tcttcgatat tccgcatatc acttaatgaa aaaacttgaa agccgcctga
    34861 atctgttaaa atcggttgat cccagttcat aaatttatgc agtccgccag cttcttctac
    34921 taagtcttca ccagggcgta gccataaatg atatgtatta cttaaaataa cgccagcacc
    34981 catttctttc aactcttctg gggacattgt ttttactgta gctaatgttc ctactggcat
    35041 gaacattggt gtggggaaag ttccgtgtgg cgtgatcaat tcccctaaac gcgcgccagt
    35101 atgtttttct ttcttgatta aacgatagcg aatggcaggt tgcgtcattc atttccctac
    35161 tttctatata aaatatcttg acaactccca ccatcataat cgtttcaagt gctttttgca
    35221 agttccctcg gcttccttct actttattta atattctttt tttatccttt ttttatgaat
    35281 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
    35341 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
    35401 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
    35461 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
    35521 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
    35581 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
    35641 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
    35701 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
    35761 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
    35821 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
    35881 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
    35941 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
    36001 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
    36061 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
    36121 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
    36181 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
    36241 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
    36301 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
    36361 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
    36421 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
    36481 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
    36541 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
    36601 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
    36661 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
    36721 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
    36781 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
    36841 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
    36901 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
    36961 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
    37021 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
    37081 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
    37141 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
    37201 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
    37261 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
    37321 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
    37381 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
    37441 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
    37501 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
    37561 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
    37621 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
    37681 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
    37741 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
    37801 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
    37861 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
    37921 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
    37981 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
    38041 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
    38101 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
    38161 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
    38221 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
    38281 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
    38341 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
    38401 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
    38461 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
    38521 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
    38581 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
    38641 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
    38701 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
    38761 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
    38821 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
    38881 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
    38941 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
    39001 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
    39061 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
    39121 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
    39181 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
    39241 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
    39301 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
    39361 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
    39421 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
    39481 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
    39541 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
    39601 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
    39661 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
    39721 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
    39781 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
    39841 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
    39901 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
    39961 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
    40021 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
    40081 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
    40141 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
    40201 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
    40261 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
    40321 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
    40381 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
    40441 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
    40501 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
    40561 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
    40621 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
    40681 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
    40741 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
    40801 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
    40861 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
    40921 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
    40981 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
    41041 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
    41101 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
    41161 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
    41221 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
    41281 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
    41341 gtgaaggccg tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg
    41401 atttcgtaga agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa
    41461 gagtcggcga agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag
    41521 aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga
    41581 atgtattaaa aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca
    41641 gtgtcggcga cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg
    41701 ataccttgca acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga
    41761 acaaagtcgt aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt
    41821 ggggagaaga tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg
    41881 aggtctgacc tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt
    41941 ttgaacatct ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc
    42001 ttggtgtctt actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg
    42061 ccttgcagac aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag
    42121 gtaaattacc tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata
    42181 cgtacattgg gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga
    42241 tgactgagtt ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg
    42301 ctggtattcg tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg
    42361 gcgctggtgg tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca
    42421 ttttggcagg tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc
    42481 tcttggaaca ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat
    42541 actaaaaatg accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg
    42601 tcttgccagc aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc
    42661 acagatctta gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc
    42721 aatcatcaac aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc
    42781 ttcaatttca gcggctcgct acacaggaac agatttaacg actacattga accttcctcc
    42841 tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg
    42901 tcaaacgtgg ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga
    42961 caccgcacaa aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt
    43021 agtagctggt gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca
    43081 agaaacttat ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga
    43141 cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc
    43201 cagttatgat ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc
    43261 tccagttgta gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa
    43321 gttaggtcat accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa
    43381 tctagtggaa ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg
    43441 gaagtgaagc aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa
    43501 aatggctcct atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc
    43561 ctctttgcag cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta
    43621 gcaaactggg tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc
    43681 tccattttaa tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg
    43741 tattccctct taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac
    43801 atccttgatg tcggcaaagg catggggatg actgctagac aacgactatt catggtagag
    43861 ttaccactct ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc
    43921 gggataaccg cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga
    43981 ggaaccaatg caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta
    44041 atggcgatca tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc
    44101 cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc
    44161 aaaggctttt ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt
    44221 atgggcaaag agttgctttt cttagctatt tatccattcc gtatactcca acttcatact
    44281 gccaacgggt tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt
    44341 tgccaatcca ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc
    44401 gtctgcttgt tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta
    44461 agccttttcc atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga
    44521 ctgccgtatc ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct
    44581 tggctaaaaa catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa
    44641 accggtacac gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc
    44701 cttcaccaga agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac
    44761 attggcgtaa aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc
    44821 cgaaagcgcc acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc
    44881 tttggatacc cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt
    44941 tccaaaagac catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc
    45001 cattgctttt ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt
    45061 cgcctcactt tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc
    45121 aattttactg attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca
    45181 ttccattcct aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag
    45241 aaatcatgca ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg
    45301 ggagactctt tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc
    45361 caggcagaat aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc
    45421 tgtctattca gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac
    45481 tgcgtgggca tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt
    45541 aaattcgttt gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta
    45601 tagttactca tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt
    45661 ctttttcgcg gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt
    45721 gaatcgctct ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa
    45781 taatctcgat ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac
    45841 gagcagtgct aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa
    45901 ttatagcgta cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa
    45961 cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc
    46021 tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta
    46081 tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg
    46141 aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga
    46201 aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa
    46261 atgattgcct ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga
    46321 ccgttgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt
    46381 cacttgacga ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa
    46441 aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg
    46501 atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata
    46561 cgctgttccc atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca
    46621 gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat
    46681 ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat
    46741 cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat
    46801 tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct
    46861 acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg
    46921 acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg
    46981 tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca
    47041 ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca
    47101 tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg
    47161 atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct
    47221 aaccgataga caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg
    47281 gcaatcgatt tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt
    47341 tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt
    47401 gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg
    47461 gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc
    47521 agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc
    47581 agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca
    47641 atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat
    47701 gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca
    47761 ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt
    47821 aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca
    47881 gccgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa
    47941 ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac
    48001 gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca
    48061 gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct
    48121 gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc
    48181 aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga
    48241 taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa
    48301 gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg
    48361 atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca
    48421 gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac
    48481 aaccttttta agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta
    48541 tagtcgaaaa tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat
    48601 aaactttaag aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt
    48661 taatacagca aaaatctaac attttttatg ggctttttct ttgaattcta taataaaagg
    48721 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag
    48781 agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
    48841 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
    48901 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt
    48961 tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg
    49021 gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat
    49081 ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg
    49141 tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca
    49201 aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt
    49261 cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt
    49321 tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga
    49381 taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca
    49441 tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta
    49501 ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca
    49561 aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct
    49621 ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac
    49681 atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat
    49741 tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca
    49801 ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg
    49861 ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt
    49921 tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt
    49981 cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct
    50041 gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg
    50101 tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca
    50161 tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc
    50221 tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa
    50281 tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga
    50341 taccagcaat gatgccattg atattattaa caacggcatc aatttgatct agcaattcat
    50401 caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca
    50461 gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat
    50521 ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt
    50581 gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat
    50641 cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg
    50701 ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg
    50761 catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt
    50821 ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt
    50881 ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct
    50941 ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga
    51001 tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca
    51061 tctcatctac tttagggatg taatccacga attcatttgc tttattcagt ttttccttga
    51121 tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca
    51181 aattttcatc tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat
    51241 cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa
    51301 attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa
    51361 tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag
    51421 taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc
    51481 cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt
    51541 tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact
    51601 ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat
    51661 cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga
    51721 tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa
    51781 gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt
    51841 accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat
    51901 atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc
    51961 ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta
    52021 tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc
    52081 ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc
    52141 aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag
    52201 attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa
    52261 ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt
    52321 ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat
    52381 gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc
    52441 ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc
    52501 taattttttt accttttcct catttttact ggatgcactg gttaagattc ggattccctt
    52561 ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac
    52621 agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg
    52681 cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc
    52741 tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc
    52801 acctacatga acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt
    52861 acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc
    52921 aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac
    52981 ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat
    53041 tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat
    53101 tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt
    53161 tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata
    53221 aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa
    53281 cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat
    53341 tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat
    53401 ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc
    53461 cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag
    53521 tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat
    53581 acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca
    53641 cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa
    53701 caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa
    53761 tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct
    53821 gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt
    53881 gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc
    53941 taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg
    54001 atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct
    54061 ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg
    54121 atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg
    54181 ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc
    54241 atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc
    54301 atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca
    54361 ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga
    54421 atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc
    54481 ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca
    54541 tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt
    54601 tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga
    54661 tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg
    54721 aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg
    54781 tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct
    54841 gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca
    54901 gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct
    54961 ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact
    55021 gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac
    55081 aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt
    55141 ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc
    55201 tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat
    55261 acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc
    55321 tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct
    55381 ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat
    55441 agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc
    55501 cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct
    55561 cttttcaata aatttagaac actctcattc taccaaatta taaccatttt gccacaaata
    55621 agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt
    55681 taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca
    55741 tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta
    55801 ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct
    55861 tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga
    55921 tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat
    55981 aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt
    56041 tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa
    56101 gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt
    56161 gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga
    56221 acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata
    56281 tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat
    56341 gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg
    56401 taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag
    56461 acgattgtta attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg
    56521 tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg
    56581 atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc
    56641 atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg
    56701 atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga
    56761 acggttccaa gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc
    56821 acaacgatat gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg
    56881 taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt
    56941 tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga
    57001 tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct
    57061 tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt
    57121 gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct
    57181 ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac
    57241 cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt
    57301 tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag
    57361 aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt
    57421 gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca
    57481 tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc
    57541 acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga
    57601 ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa cacaggtagt
    57661 tgggggtatt tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa
    57721 atcatggcga atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac
    57781 tctttcccta attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga
    57841 tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc
    57901 aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca
    57961 tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac
    58021 gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct
    58081 tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca
    58141 tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac
    58201 ggttcatctg cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt
    58261 ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt
    58321 gtgattgctg cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga
    58381 tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa
    58441 ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac
    58501 aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg
    58561 acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga
    58621 tctgctacag gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat
    58681 gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca
    58741 gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta
    58801 atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga
    58861 ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt
    58921 cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc
    58981 ggttacgcaa tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag
    59041 attgacgctg atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt
    59101 atttcaagcc catattttcc cataagagtg ggtacatact gaactccgta aatcctggca
    59161 tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc
    59221 tcaatccgct tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat
    59281 aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg
    59341 cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt
    59401 cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg
    59461 ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg
    59521 cttttgtaat gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca
    59581 aagaaccttc acattgatca aatacttttt taggattttc tttccattga ttcttaagta
    59641 ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt
    59701 tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct
    59761 ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga
    59821 tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg
    59881 tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta
    59941 gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat
    60001 aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg
    60061 cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc
    60121 gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat
    60181 aaaaaaacga gccagcttct aaaagaacga gcgtcattta ttttttgtca gctagattat
    60241 ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac
    60301 aaatccacgc agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc
    60361 aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc
    60421 caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt
    60481 cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa
    60541 aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa
    60601 atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga
    60661 cgcggtagtc gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca
    60721 actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct
    60781 aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga
    60841 aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc
    60901 caagatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa
    60961 aaacttgaat catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca
    61021 aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt
    61081 gtttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact
    61141 aacaaaagaa gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt
    61201 cggttccttt gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat
    61261 caactacatc gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac
    61321 acaagccgtt ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg
    61381 actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt
    61441 tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca
    61501 tgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct
    61561 gaaagcattt gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat
    61621 ggaagtcgct tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac
    61681 attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta agatccctca
    61741 acagctccac catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt
    61801 gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg
    61861 tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga
    61921 aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat
    61981 tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa
    62041 ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct
    62101 caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat
    62161 aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg
    62221 aacacattta tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc
    62281 ttcgttacct actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt
    62341 gacgaaaaga catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag
    62401 cttttcttaa ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt
    62461 caaattcttt tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta
    62521 aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat
    62581 ccgcactatc agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg
    62641 tcgtataatg gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt
    62701 ttatgacatc ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc
    62761 gccttcttcc ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc
    62821 cattttgcca agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg
    62881 atcaataata gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc
    62941 aataaaaact ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg
    63001 cgatccattg gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt
    63061 actacgtgcc agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg
    63121 accaataaag aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt
    63181 ttcaaaatcc atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac
    63241 ataaaaatcg aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa
    63301 aaaaggaaat tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac
    63361 ttccattcct tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa
    63421 ccataaatag caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga
    63481 aacaataagc caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt
    63541 ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg
    63601 acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca
    63661 atcgcttgat acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata
    63721 ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat
    63781 gctgcactca gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct
    63841 aatattccaa gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa
    63901 ctatctagac caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa
    63961 aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa
    64021 agataaggaa taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta
    64081 cgcccaaatc gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta
    64141 atctgctctt cgattttagc attaaagggg aaataaacca attttacaga atatattctt
    64201 gtaaaaaaaa atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta
    64261 ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt
    64321 gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca
    64381 gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga
    64441 gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga aataagttct
    64501 tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc
    64561 actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa
    64621 actgaagaag tcaatccttg accacgtact ttatttagca aatataattt acttaaataa
    64681 atttttccat ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat
    64741 aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt
    64801 ttcagcatgt aatccacttg tttttgacca atgatcggca caaaaacttc cggccatact
    64861 tcttgtatga ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata
    64921 cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg
    64981 atcatgctat acacacccac cgaccacggt ttcaagaaaa tcacaatctg cataaattcc
    65041 ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga
    65101 agcagcaagg taacaactag tactttttta atatctttcg tttttcttgt cagcaattca
    65161 tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa
    65221 cggacaagca aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc
    65281 ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata
    65341 ggaatgatca cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat
    65401 tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa
    65461 agaatcaaca caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt
    65521 aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt attaaaactg
    65581 cctcctt
//
LOCUS       DAPBCS010000007        65550 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPBCS010000007 DAPBCS010000000
VERSION     DAPBCS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 65550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.007"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            202..720
                     /locus_tag="RD521_000584"
     CDS             202..720
                     /locus_tag="RD521_000584"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7770345.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            848..1531
                     /locus_tag="RD521_000585"
     CDS             848..1531
                     /locus_tag="RD521_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDT7770346.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1840..1913
                     /locus_tag="RD521_000586"
     tRNA            1840..1913
                     /locus_tag="RD521_000586"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1874..1876,aa:Arg,seq:tct)
     gene            2072..2431
                     /locus_tag="RD521_000587"
     CDS             2072..2431
                     /locus_tag="RD521_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDT7770347.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2554..4185
                     /locus_tag="RD521_000588"
     CDS             2554..4185
                     /locus_tag="RD521_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDT7770348.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4247..4888)
                     /locus_tag="RD521_000589"
     CDS             complement(4247..4888)
                     /locus_tag="RD521_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDT7770349.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5074..5150
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5170..5478
                     /gene="rplU"
                     /locus_tag="RD521_000590"
     CDS             5170..5478
                     /gene="rplU"
                     /locus_tag="RD521_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDT7770350.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5505..5849
                     /locus_tag="RD521_000591"
     CDS             5505..5849
                     /locus_tag="RD521_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDT7770351.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5862..6155
                     /gene="rpmA"
                     /locus_tag="RD521_000592"
     CDS             5862..6155
                     /gene="rpmA"
                     /locus_tag="RD521_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDT7770352.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6589..9396
                     /locus_tag="RD521_000593"
     CDS             6589..9396
                     /locus_tag="RD521_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7770353.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9587..10078
                     /locus_tag="RD521_000594"
     CDS             9587..10078
                     /locus_tag="RD521_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7770354.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10181..11170
                     /gene="manX"
                     /locus_tag="RD521_000595"
     CDS             10181..11170
                     /gene="manX"
                     /locus_tag="RD521_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDT7770355.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11203..12006
                     /locus_tag="RD521_000596"
     CDS             11203..12006
                     /locus_tag="RD521_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDT7770356.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12027..12941
                     /locus_tag="RD521_000597"
     CDS             12027..12941
                     /locus_tag="RD521_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDT7770357.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13024..13398
                     /locus_tag="RD521_000598"
     CDS             13024..13398
                     /locus_tag="RD521_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDT7770358.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13537..13782
                     /locus_tag="RD521_000599"
     CDS             13537..13782
                     /locus_tag="RD521_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7770359.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13954..15018
                     /locus_tag="RD521_000600"
     CDS             13954..15018
                     /locus_tag="RD521_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDT7770360.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15264..15689
                     /locus_tag="RD521_000601"
     CDS             15264..15689
                     /locus_tag="RD521_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7770361.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15682..16131
                     /gene="nusB"
                     /locus_tag="RD521_000602"
     CDS             15682..16131
                     /gene="nusB"
                     /locus_tag="RD521_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDT7770362.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16228..17082
                     /locus_tag="RD521_000603"
     CDS             16228..17082
                     /locus_tag="RD521_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDT7770363.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17083..18420
                     /locus_tag="RD521_000604"
     CDS             17083..18420
                     /locus_tag="RD521_000604"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDT7770364.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18425..18649
                     /locus_tag="RD521_000605"
     CDS             18425..18649
                     /locus_tag="RD521_000605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDT7770365.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18651..19541
                     /locus_tag="RD521_000606"
     CDS             18651..19541
                     /locus_tag="RD521_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDT7770366.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19550..20368
                     /locus_tag="RD521_000607"
     CDS             19550..20368
                     /locus_tag="RD521_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDT7770367.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20475..20924
                     /locus_tag="RD521_000608"
     CDS             20475..20924
                     /locus_tag="RD521_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDT7770368.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20942..22624
                     /gene="recN"
                     /locus_tag="RD521_000609"
     CDS             20942..22624
                     /gene="recN"
                     /locus_tag="RD521_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDT7770369.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22679..22795)
                     /locus_tag="RD521_000610"
     CDS             complement(22679..22795)
                     /locus_tag="RD521_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDT7770370.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22868..23824)
                     /locus_tag="RD521_000611"
     CDS             complement(22868..23824)
                     /locus_tag="RD521_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDT7770371.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23995..24363
                     /locus_tag="RD521_000612"
     CDS             23995..24363
                     /locus_tag="RD521_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDT7770372.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24533..24964
                     /gene="mraZ"
                     /locus_tag="RD521_000613"
     CDS             24533..24964
                     /gene="mraZ"
                     /locus_tag="RD521_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDT7770373.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24985..25944
                     /gene="rsmH"
                     /locus_tag="RD521_000614"
     CDS             24985..25944
                     /gene="rsmH"
                     /locus_tag="RD521_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDT7770374.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLRQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25949..26350
                     /gene="ftsL"
                     /locus_tag="RD521_000615"
     CDS             25949..26350
                     /gene="ftsL"
                     /locus_tag="RD521_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDT7770375.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26351..28543
                     /locus_tag="RD521_000616"
     CDS             26351..28543
                     /locus_tag="RD521_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDT7770376.1"
                     /translation="MSLKNKIWRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28569..29531
                     /locus_tag="RD521_000617"
     CDS             28569..29531
                     /locus_tag="RD521_000617"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDT7770377.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29545..30906
                     /locus_tag="RD521_000618"
     CDS             29545..30906
                     /locus_tag="RD521_000618"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDT7770378.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30932..32020
                     /gene="murG"
                     /locus_tag="RD521_000619"
     CDS             30932..32020
                     /gene="murG"
                     /locus_tag="RD521_000619"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDT7770379.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32035..33255
                     /locus_tag="RD521_000620"
     CDS             32035..33255
                     /locus_tag="RD521_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDT7770380.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33411..34736
                     /gene="ftsA"
                     /locus_tag="RD521_000621"
     CDS             33411..34736
                     /gene="ftsA"
                     /locus_tag="RD521_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDT7770381.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34759..36000
                     /gene="ftsZ"
                     /locus_tag="RD521_000622"
     CDS             34759..36000
                     /gene="ftsZ"
                     /locus_tag="RD521_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDT7770382.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36017..36694
                     /locus_tag="RD521_000623"
     CDS             36017..36694
                     /locus_tag="RD521_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDT7770383.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36708..37307
                     /locus_tag="RD521_000624"
     CDS             36708..37307
                     /locus_tag="RD521_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDT7770384.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37322..37609
                     /locus_tag="RD521_000625"
     CDS             37322..37609
                     /locus_tag="RD521_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDT7770385.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37658..38440
                     /locus_tag="RD521_000626"
     CDS             37658..38440
                     /locus_tag="RD521_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDT7770386.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38461..39168
                     /locus_tag="RD521_000627"
     CDS             38461..39168
                     /locus_tag="RD521_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDT7770387.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39430..42228
                     /gene="ileS"
                     /locus_tag="RD521_000628"
     CDS             39430..42228
                     /gene="ileS"
                     /locus_tag="RD521_000628"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDT7770388.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42277..43803)
                     /locus_tag="RD521_000629"
     CDS             complement(42277..43803)
                     /locus_tag="RD521_000629"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDT7770389.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43818..44465)
                     /locus_tag="RD521_000630"
     CDS             complement(43818..44465)
                     /locus_tag="RD521_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDT7770390.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44649..44978)
                     /locus_tag="RD521_000631"
     CDS             complement(44649..44978)
                     /locus_tag="RD521_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDT7770391.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45155..45883
                     /gene="sfsA"
                     /locus_tag="RD521_000632"
     CDS             45155..45883
                     /gene="sfsA"
                     /locus_tag="RD521_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDT7770392.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45899..46912
                     /locus_tag="RD521_000633"
     CDS             45899..46912
                     /locus_tag="RD521_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7770393.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46912..48189
                     /locus_tag="RD521_000634"
     CDS             46912..48189
                     /locus_tag="RD521_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDT7770394.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48252..50954
                     /locus_tag="RD521_000635"
     CDS             48252..50954
                     /locus_tag="RD521_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7770395.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51106..51423
                     /locus_tag="RD521_000636"
     CDS             51106..51423
                     /locus_tag="RD521_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7770396.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51453..51773
                     /locus_tag="RD521_000637"
     CDS             51453..51773
                     /locus_tag="RD521_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7770397.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51881..53341
                     /locus_tag="RD521_000638"
     CDS             51881..53341
                     /locus_tag="RD521_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7770398.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53409..53630
                     /locus_tag="RD521_000639"
     CDS             53409..53630
                     /locus_tag="RD521_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770399.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53661..53843
                     /locus_tag="RD521_000640"
     CDS             53661..53843
                     /locus_tag="RD521_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDT7770400.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53843..54256
                     /locus_tag="RD521_000641"
     CDS             53843..54256
                     /locus_tag="RD521_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDT7770401.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54379..55560
                     /locus_tag="RD521_000642"
     CDS             54379..55560
                     /locus_tag="RD521_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7770402.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55631..55795)
                     /locus_tag="RD521_000643"
     CDS             complement(55631..55795)
                     /locus_tag="RD521_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770403.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55949..56022
                     /locus_tag="RD521_000644"
     tRNA            55949..56022
                     /locus_tag="RD521_000644"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55983..55985,aa:Arg,seq:cct)
     gene            complement(56091..57230)
                     /locus_tag="RD521_000645"
     CDS             complement(56091..57230)
                     /locus_tag="RD521_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7770404.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(57529..58164)
                     /locus_tag="RD521_000646"
     CDS             complement(57529..58164)
                     /locus_tag="RD521_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770405.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(58277..58912)
                     /locus_tag="RD521_000647"
     CDS             complement(58277..58912)
                     /locus_tag="RD521_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDT7770406.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(58946..59407)
                     /locus_tag="RD521_000648"
     CDS             complement(58946..59407)
                     /locus_tag="RD521_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDT7770407.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(59537..59968)
                     /locus_tag="RD521_000649"
     CDS             complement(59537..59968)
                     /locus_tag="RD521_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDT7770408.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(59986..60306)
                     /locus_tag="RD521_000650"
     CDS             complement(59986..60306)
                     /locus_tag="RD521_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7770409.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            60605..61381
                     /locus_tag="RD521_000651"
     CDS             60605..61381
                     /locus_tag="RD521_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDT7770410.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            61396..61599
                     /locus_tag="RD521_000652"
     CDS             61396..61599
                     /locus_tag="RD521_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770411.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            61615..61935
                     /locus_tag="RD521_000653"
     CDS             61615..61935
                     /locus_tag="RD521_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDT7770412.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            61940..62110
                     /locus_tag="RD521_000654"
     CDS             61940..62110
                     /locus_tag="RD521_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770413.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPVGRD"
     gene            complement(62117..62899)
                     /locus_tag="RD521_000655"
     CDS             complement(62117..62899)
                     /locus_tag="RD521_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770414.1"
                     /translation="MDISDTPYYQKLIASSKLIGETIAPSLDVVNAMQPAIEQAKKLI
                     AIQDTFIASQAILNDSLIQIVEKRNKSIANMIPPSLFAIQKQLESPSFNIAKKMAATM
                     PKPYFSDISKITEKFAVDMSKYDFRIGVATQELEKAFSSEPEVSTKNLLFKDTSNPSD
                     EFEAKFFDILNEQRKVLDDSIKLFEEINTAKNKDITNEPSEKQQASPAFYKDKMWYLE
                     QTGAALIGLVVTEIVNITIGVDPNNTISLALFLRYLLQFLIN"
     gene            62950..63657
                     /locus_tag="RD521_000656"
                     /pseudo
     CDS             62950..63657
                     /locus_tag="RD521_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
     gene            63835..64176
                     /locus_tag="RD521_000657"
     CDS             63835..64176
                     /locus_tag="RD521_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770415.1"
                     /translation="MEKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            64169..64840
                     /locus_tag="RD521_000658"
     CDS             64169..64840
                     /locus_tag="RD521_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDT7770416.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            64846..65532
                     /locus_tag="RD521_000659"
     CDS             64846..65532
                     /locus_tag="RD521_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770417.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
BASE COUNT        21744 a        10985 c        13703 g        19118 t
ORIGIN      
        1 ctttctaaaa cttgtaatgt tagggtaaac gttttttaaa ctaacgtcaa ttatttataa
       61 taaaaaatga gataaagatg aggaaataga attagaaaca tataattatt aaagggaaac
      121 atttttacag ctataatttg aaaaaaattt atcgttatta tatgattaag aaggattgaa
      181 taaagaaaga agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata
      241 acgttgatta tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt
      301 ttgactcctt atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg
      361 atatatgtac gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat
      421 gaaaattcag gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa
      481 gatttttata caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga
      541 aatattatcg gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg
      601 gcatttgcat ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca
      661 gcatggaata cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa
      721 gaatgttaaa aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg
      781 agaaaacact catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag
      841 gagtagtatg cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt
      901 tatgcgtacc cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca
      961 tgtagcagaa gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg
     1021 acagcttcag cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt
     1081 catggccacg acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat
     1141 gatcgagaaa gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct
     1201 tgaaggtcaa gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat
     1261 ggatagtgca aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa
     1321 aggtggcgtt ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga
     1381 agagattccc cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt
     1441 gatggatcat cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat
     1501 gacaaaggaa tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc
     1561 gattgaatcg agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt
     1621 aaaggataca ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt
     1681 caattgataa ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta
     1741 gttgggccaa aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca
     1801 atttaattat ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat
     1861 agagcatccg ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa
     1921 accgttagaa actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt
     1981 tttcaaaggc aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa
     2041 aataaaactc atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac
     2101 tctagttgca attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga
     2161 cacgacaagt agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac
     2221 attgtttaag aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc
     2281 ttatctttct tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt
     2341 tgcattatat ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc
     2401 tctgttggca ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca
     2461 ggaaactttg tgaaaaatat tactttatct catgaaatat gatattctaa tgaatggtat
     2521 tttagaaaat ttcatgaaaa aggagttaga taggtgttca aaaattatgt ttcctcacta
     2581 aaaaaagaat tttcaggtta tgatctttca agatttcaga aagatctttt ggctgggatc
     2641 acagtagctg ctgtagcatt gcctttagcc ttggcttttg gggtatcaag cggagctgat
     2701 gcggccgcag gtttgattac ggctatcatt gccggtatcg ttattggcgg tttatcaggc
     2761 ggtttttatc aaatatcagg tccgacaggt gcgatggcag ctatcttgat gtctattgcg
     2821 gcaacaaagg gaatgcaagg tgtattgctt gctacttttt tagcgggtct atttttactt
     2881 gcagcaggac ttttccgatt aggaacactg acttctttta tcccagcacc cgtaattacg
     2941 gggtttactt caggtattgc tatcattatt gcattaggtc agatcgataa tttatttggt
     3001 gttcactcgg aaggtacaaa cgttattgaa aaactaagta gttatcaaac gataggattt
     3061 ccgataaaca cagcctcttt gatcatggga gggctagtca ttttagggat gtttatctat
     3121 ccaaaaaaat gggcgaaacg aatgcctagt tcgctgctgg ctatcatttt ggcaacagca
     3181 gttatgctat tgttccattt accagtagca actgtgggta agattccaca aacattgatc
     3241 agtagtaatc gtttgaatat gggagatttt agcttttctg ccttgcaaca agtggcagtt
     3301 ccagctatta gcattgcttt attaggaatg attgaaagct tgctatgtgg tgcttcggct
     3361 ggccgaatgg caaataagcc cttagatagc aatcaagaat taatggcgca aggaattgga
     3421 aatctgttgc ttcctttctt tggcgggatt cctgctactg cggccatcgc ccgtacaagc
     3481 gtggccatca aatctggtgc acaaactaga ttagctggta tgatacatgc agttgtctta
     3541 ctgctctcca tgcttgtttt tgcgccagtg atgtcacaga ttccaatgcc agcacttgca
     3601 ggtgttttga ttgtgacggc ttggcgtatg aatgaatggg aaacgatcaa ggagctattt
     3661 actaataaat attggtcagc agttctattg tttatcctta cgatgatttg tacagttatc
     3721 tttgatttaa gtattgctat cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc
     3781 aaaagcgctg ctatcacgat ttcattagaa acgatcgatt ggcaaagaat ggggctgcca
     3841 gaaacggaaa agctgaataa ctggacagta gtgtatatta gtggtccact gttttttatg
     3901 tcttcagaaa aactcaaaaa taaattggaa gaactaaatc atcacgatgg tgtcattttc
     3961 tccatgcgag gcgtaccaag tattgatttg acggcacgtg cagtttttga tgaatttcaa
     4021 gaaaaagcag agcaaagagg acaaacgatt atctatactt ccttgcagcc agaagtagaa
     4081 aaacaacttt ctcacttgtg ggaacataca ttcacgcatc aacatcaaac agttgctcat
     4141 gcgttgacag ctttacatgc gaaatatact gcaattagcc attaaaacaa tgaaaaagaa
     4201 cagacaagac tgtaaactga agattttatg gtcttgtctg ttctttttaa tattgctgta
     4261 aaagtgaagc tagtacttgc cagtcttgat ggataacagg tacaagggag cgattgtaga
     4321 aaatagctgc tggatgaaaa agtggaagaa ttcgatagtt tttttctgac cattcgtagg
     4381 catccttttg ttctgttaat tgtaagacag gccctgtata gagttgacca tgatttttgg
     4441 taaccgtata ttctttgcca agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta
     4501 attttggctg gaccttcatc agttcgtaat ccaatattgg tgcatgcgca aatacttcgg
     4561 aacgagtagg tgtccgattt ggataaacag tttctgactg ttgtgtccgt ttattgatcc
     4621 gatgagtcac gcgatacggg cggctcctta cagcactggt gatatacacc tcttctcgtg
     4681 ttaagccagt tgaagaaagt gcctgcatca attcttttcc agcttgaccg ctaaaaggaa
     4741 catgcgtatc aatctctgtt ttccctgggg cttctcccac gagcatcagt ttaggatgaa
     4801 gaggtccttg cccagcgata aatccttcta caggacggcc gttcattctt aaagaagctt
     4861 cttgtatcag ttcttttggg tattccattt atgctcctcc tttttctttt attataagaa
     4921 taaaagaaac acaatgcgac tattttattt ggtagaactg tgaggataga aaagcagaag
     4981 tattgcttga atttttgttt tactttttga ttttaaattt agttgactaa cggttgctgt
     5041 tctggtaagc tagtactgtt gtaattgtgg actccacagc tacaaccgct cagaacaagt
     5101 aataagcaca gcatatgctg tcacttcgga tggcgagtct aagtatatca gaaggaggtg
     5161 ctttatagca tgtacgcaat tattaaaact ggtggtaaac aagtaaaagt tgaagtggga
     5221 caagcaatct atgttgaaaa acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa
     5281 gttatcttag taggcggaga atctacaaaa gtaggcgctc caactgttgc aggtgcaact
     5341 gttgaaggta ctgttgaaaa acacggcaaa caaaagaaag tcgttacttt caaatacaaa
     5401 cctaaaaaac attcacaccg taaacaaggt catcgtcaac catatacaaa agtggttatc
     5461 gacgcgatca acgcttaata gcgtttgaga aagaaggcct atatatgatc aaaggaacat
     5521 ttaagcgaaa tgacgcaggt cagatcgttt cattcacttt gaccgggcat gctgatgctg
     5581 gtccttatgg aagtgatatc gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca
     5641 acgggattgc ctcattagcc ggttttgagc cgattgttga aatgaatgaa gaagaaggcg
     5701 gttatcttta taccgaagtc acttcaggaa tgacacaaga acaaaacaat attgctgaga
     5761 ttctattaga aaatctttta ttaggattac aatcgataga agctgaaaac tcagaatata
     5821 tccaaattaa aacaattaat gataaatagg aggtgcagac catgttgtta tctatgaatc
     5881 tacaattatt tgcccacaaa aaaggtggcg gttctacatc taacggccgt gactcagaat
     5941 caaaacgtct aggtgctaaa cgtgctgatg gacaaacagt tacaggtgga tcaattcttt
     6001 accgtcaacg cggtacaaaa atctatccag gtgtaaacgt gggtatcggt ggagacgaca
     6061 ctttatttgc aaaagttgat ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac
     6121 aagtgtctgt ttatccagta gctcaagaag cttaattgaa tcttatgatt aaagaggttg
     6181 tgacaagact tttgtcacag ccttttttgt ttataaaaat aaaagttgta gtggaggcta
     6241 cgaagtttac tttgtctttt tacacttaat atttttttgc caatttggaa tcaggatgtt
     6301 ttgataacta tcgttttcaa ttgatggaag ctatattgtt tgctatcagt gaaataatta
     6361 tatggtaaac agtgttcttt ttcttgtttc cattataaaa atcgtatcgg gatttttcgt
     6421 tcttttaaat tgaacatttc tatctatccg cgtgttatca acaaaacaaa atcaggtaaa
     6481 tagttgacga aacagaaata aatacatagt attatgtttt gaagcctaag tacaatagtc
     6541 tatagaaaag gagcgatacc attggcaaaa cgcattgacc gaatttatat gtatgtcaaa
     6601 gaaaaaaccg ctcatttaac aaaggcagaa tatgatcaag gagtaacaac acaggaaatt
     6661 gctgaagtgt tagggattca gcgaacaaat agcagtaaag atctgaatca gctagtgcgc
     6721 gaaggaaaac tgataaagac agacggacga ccagtacgtt acatatatca aacactcgtt
     6781 acgcacgtaa agccagttac aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa
     6841 gaaaaaaata cactccaaat agatactaag gatatctttg caaaaatcat tggagctaac
     6901 ggcagcatga aaaattcagt ggaacaggca aaagctgcga ttttgtatcc gccaagagga
     6961 ttaaattgtt tgattacagg acctaccgga tcaggaaaga cttattttgc acacgcgatg
     7021 tatcattttg caaaagcgca tcatgtaata gatgaggaaa cggaactgat tgttttcaat
     7081 tgtgctgatt atgccaataa ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa
     7141 ggtgctttta caggcgcaga tgaagaaaaa atggggatta tcgatcaagc ggatggcggt
     7201 atgctgtttt tggatgagat tcaccggttg ccgcctgaag gtcaagaaat gatcttttac
     7261 tttatggatc atggaactta cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat
     7321 gtacggatcg taggtgcgac cacagaagac ccaggttctt ctctattaga aacttttgtt
     7381 cggcgtatcc caatcaatat caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa
     7441 gtcgatttag taaaaatcat gattgcccac gaagcaaacc gcacgcagcg aaagatttct
     7501 ttgacagaag atgtagcaaa agcattgatc ggcagtgtga cctatggaaa tataggacaa
     7561 ttgaagtcaa atatccagtt agtatgtgca cgaggatttc taaatcatat gcaatcaccc
     7621 gaaatctcga tcacaatcga tgatttgaca gaagggattc gtagcggatt gattcaatta
     7681 gcaagcaacc gcagtgcaat ggcagagctt tctaaaatat tggagccaaa gataactgta
     7741 gcaccaaatg atacgatcat gaaaatccaa tcggactcct atgagttacc ttataatcta
     7801 tatgatatca tcggagataa agcagcttta ctgaagtcgg atggtttgga tcaagaagca
     7861 atcaatcatt tcatttctac agatatcaac atccatctga agtctttcta taaagaccat
     7921 ggtttttctt ttaatgcaga cagcaaatta gcagaatttg tagatccgaa aatcatagaa
     7981 gtgaccaatc agatttatgt aatggtcaaa agatctttac catatgagtt tcagcaaaac
     8041 tttatttatg ctatgagctt gcatatcagt tcttttttga aaaaaatcaa cttaggagaa
     8101 gaacggcata cgaatgataa catccgtgag atggcttttg attttccaga tgaatatgct
     8161 gttgcgaaag aagtgcgtag ttatatcgaa acgtattttc aagtggagat tccagaaagt
     8221 gaagactatt acttgaccgt tctactcgtc tcgttacggg caaatcaagc aagcggcaga
     8281 atcggtgtag tcgttgcagc tcacggaaac agcacagcaa gcagtatggt ccaagttgtc
     8341 caacagttgt tagatgcaga gaacgtacga gcagtagaca tgcctttgga tatggatccg
     8401 aaaacagcat tggaccgaat tgaacgcaat gtacaagaag tagatgaagg aagcggggtc
     8461 atactcttag tagatatggg gtcccttgcg tcgtttaatt cgcaaattca aagagacact
     8521 ggaattccag tacgtactgt tgatatggta acaacttctc tagttttgga aactgtacga
     8581 aaggtctcag tattagggac agatctagat atgttgcatg aatcattgaa aaattttcga
     8641 ggttacgcag aaatatcttc tgtagaatct gttcaggaag caaaacctat tcataagaaa
     8701 aaagctgttt tggctatctg cgcatctggc gaaggtacag cacaaagaat caaagaattg
     8761 atcgaacgag cggtaaacaa gcgtcaagaa acagaattag aagttctagc tttgtcgatt
     8821 gtggaagtga aagaagaact gccagttatc caaaaagaat atcagatcat agctactact
     8881 gggattactg atttgaatat ccaagcccca tttattcctt tagaacgttt tattgatcaa
     8941 aatatcgaag tcattttgga tcagttgctg atggaatcag aattggagga aactgaattc
     9001 atctccctag atgaagagtc agctaaaaat acgtgtgttg agtttatctc tgataacttc
     9061 atctttatta atggtagtaa gttaatcgat ccaatgtggc aattttctac acaaatctct
     9121 cagacgacgg gaatcggcga tgaagaatat ggattcaaaa tcaacctagt gatgcacaca
     9181 gcaggaatga tcgaacgtat tattcggaat gaaccattga ctgtggaaga aaacgaattg
     9241 acaaatacaa cgaatgatcc tctttatagc caattggcag cctcagttgt tctgttggaa
     9301 gatcagataa aagtaaaagt gccgatagaa gaaatgtatt atctgctacg tctagtccat
     9361 aatcagttag ataaaaaaga atacactgtg ccatgaatac acaaaaacga tacactttta
     9421 tagtgtatcg tttttgtgta ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa
     9481 aaagttggca cgctttttgc ttatagtagt gtgtgctagg aggtggttgt tcacacagaa
     9541 atgatagggg tttctgtgaa gtagtgagga agaaggagtt ttgcagatgg atattcgatt
     9601 ggcacgtata gatgatcgtt taatccacgg gcaagtcgca acagtttggt cgaagcaaac
     9661 aggtgttgaa cgtatcattg tgatcagcga tgtagtcgct caagataaat tgagaaagtt
     9721 tctactaaaa gaagctgcac cgccggggat cacagcaaat gtaataaccg tgcagaaaat
     9781 gattcaaatc tatcatagtg gattattatc acgagcaaaa gtgatgcttt tattcaccaa
     9841 tccccaagat gttgaagcgg ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg
     9901 cggtatgagt tttactgaag gcaaatcaat gattaccaat tttgtttcag taaatcaaca
     9961 agatattgat tcctttcgtt acttgaacag tcaagaagtg gagttagaga ttcgaaaagt
    10021 acctgctgac aggaaaatcc ttttgatgga actactagcg aaaatcaaaa aggtgtagca
    10081 agaatggttg ttggaaaagg tcaaataatt ctgttgtaat ggttagcaat tattaatatt
    10141 tttgaatttg ttacaacaaa agtaaatagg aggtataaat atggtaggga ttatcctagc
    10201 aagtcatgga caatttgctg aaggaatctt acaatccggt tcaatgatct ttggcgaaca
    10261 agaaaacgtc aaagccgtga ttttgaaacc aagtgaaggt ccagatgact tgagagccaa
    10321 attggaagaa gcggttgctt cttttgataa tcaagacgaa gtgttattct tagttgattt
    10381 atggggcgga acaccgttca atcaatcaaa cacattattc gaagagcaca aagataaatg
    10441 ggcaatcgtc agcggtttga atttaccaat gttgattgaa gcctacgcat cacgcttttc
    10501 aatggaatct gctcatgaaa ttgcagcaca catcattgaa acagcaaaag acggtgtaaa
    10561 agtaaaaccg gaagaattag aaccggcaga agcaccgaaa gcagcagttg aagacgcaca
    10621 acctaaagga gcacttccag aaggaactgt ggtcggagac ggaaaaatca aatatgtatt
    10681 agcacgtgtt gactcacgtc tgttacatgg acaagttgcg acagcttgga caaaagcagt
    10741 gcaaccaaac cgtattatcg ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag
    10801 actaatcgaa caagctgctc ctccaggtgt aaaagcaaat gttattccta ttagcaaaat
    10861 gatcgaagta gcaaaagacc ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa
    10921 tcctgaagac gtattgacag ctgttgaagg tggcgtagac atcaaagaat taaatgttgg
    10981 gtcaatggcg cattcggtcg gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga
    11041 agacgttgaa gcgtttgaga agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt
    11101 acctaatgat tcacgcgata acatggatga tattttgaaa aaagcaaaag ctgaattagc
    11161 aaaagcgtaa taaataatat taaaatttat aggaggctta tcatgtctat tatttcaata
    11221 attttagtcg tattagttgc ctttctagct ggtatggaag gtattcttga tgaatttcaa
    11281 tttcatcaac cactagtggc atgtacattg atcggtttag ttaccggtaa cctcgaagct
    11341 ggtatcgtac ttggcggtac gcttcagatg atcgctttag gttgggcaaa tatcggtgcc
    11401 gctgtagcac cagatgctgc attagcatca gtagcatcag caattatttt agttttaggt
    11461 ggacaaggtg ttaaaggtgt tccttctgcg atcgcgattg ccgttccttt ggctgttgca
    11521 gggttgttct taacaatgat cgtacgtaca atcgctgttc caatcgttca tttgatggat
    11581 gcggctgctg aagaaggaaa catccgtaag gttgaaatgt ggcacatcat tgctgtatgt
    11641 ttacaagggg ttcgtatcgc ggtcccagca gcagcgttgc tatttatccc agcacaaact
    11701 gtgcaatcat tccttgaatc aatgccagca tggttaacag atggtatggc tatcggtggg
    11761 ggaatggtcg tagctgtagg ttacgctttg gtaatcaaca tgatggctac aaaagaagta
    11821 tggccattct tcgttattgg tttcgttgta gctgcaattt ctcaattaac attgattgct
    11881 ctaggtgctt taggtgttgc attagctctt atttacttga acctttctaa aatgggtggt
    11941 tcatcaaata atggaggcgg aagcaactct ggtgaccctt taggcgacat tttgaatgac
    12001 tattaatctt gaaggaggaa aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag
    12061 atcgtttagc tgttgcgtgg cgctctacct tcattcaagg ttcatggaac tacgaacgta
    12121 tgcaaaacgg tggctgggca ttttctatga ttccagcaat caaaaaatta tacaaaacaa
    12181 aagaagaacg ttctgcagca ttaaaacgtc acttggaatt ctttaataca cacccataca
    12241 ttgcttctcc aattttaggg gttacattgg ctctggaaga agaacgtgcg aatggcgcgc
    12301 ctgttgatga tgtagcgatt caaggggtta aagtaggtat gatgggtcct ttagctggtg
    12361 ttggtgaccc agtgttctgg tttacagtac gtccaatgct aggtgcacta ggtgcttcat
    12421 tagctatggg aggaaatatc cttggaccaa tcatcttctt cttaggttgg aacattattc
    12481 gttgggcatt catgtggtat acacaagaat ttggctacaa agctggttct aaaattactg
    12541 atgacctttc tggcggattg ttacaagacg taacaaaagg tgcttcaatt ttagggatgt
    12601 tcgtattagc tgcccttgta caaagatggg tatcaatcaa attcttacca gttgtatcaa
    12661 ctgtaaaatt agataaaggt gcttacatcg aatgggataa attgccttca ggtggtgaag
    12721 gaatcaagag tgcatttgaa caagttaata atggcttagc cctttctcct gaaaaggtaa
    12781 caacattaca agacaactta gaccaattga ttcctggttt agctgcatta ggattaacat
    12841 tgttatgtat gtggttgctt aagaaaaagg tttctccaat cgttatcatc cttggtttat
    12901 tcgtagtcgg tgttgttgga cacgtcatcg gacttttata atccataaaa atatagctcg
    12961 ggcgttgtcc cgagcttttt ttatatagta tactgttcat aaacaaatat ctggagggag
    13021 aaaatggttc aatcaatcaa tacaaaagta gacttggtaa ttgatgctac ttcgtttacg
    13081 ggtctgacag attacgggca aatcatgata ggtgataatg gttttgagtt ttaccatgcg
    13141 cgagatagac gcaaatatat ccaaattcca tgggaagaag tggattatgt actagcatca
    13201 gtcatgctaa agggaaaatg gattcctcga tatgccattc aaacgaaaaa aaatggtaca
    13261 tacacatttt cttccaaaga acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat
    13321 gaagatagaa tggtacaatc attgagtttc tttgatgtag tgaaacgtgg tattcgtagt
    13381 atgtttaaac gcaagtaaaa aataaagaaa acttctaaga ggaggttttc tttatttttt
    13441 tactttgcag aagaaaatat gaaatagcaa aaccaattat ttgtcccctt taaatcccat
    13501 atgctaaaat tgattagcaa agaaggaggt tatattatga gttggttatg gacattgatt
    13561 gttggagccg tgatcggagc aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc
    13621 tgtgtaacga atatcattgc tggactagtc ggttcaagta ttggacaagc gattttcggc
    13681 gaatggggac caaaagctgc aggtatggca cttattccat ctattttagg cgcagtgatt
    13741 ttggtagcag ttgtctcatt tttctttgga aaacgatcat aagtttttac aaaatagtaa
    13801 actaaaagga gttgcagcga gactgtgact cctttttggc tttatttatt taaaataaaa
    13861 agaagttttc cggaaatttt tatttaactg tcgtgtacga agaaaagttt gctataatgg
    13921 aaaaaagcgt gcaaagaaag agggagtttc attatgatgc tacgtgtaga aaaattgcgc
    13981 aaaaaaatgc aagaagaaaa tttggattca tttttggtta ccagtccgta caatcttcgt
    14041 tacttaacga actttacagg tacgacagga ttagctgtta ttactttgga aaaagcattt
    14101 tttattactg attttcgtta taccgagcag gcggcagcgc aggctcaagg gtttgaaatt
    14161 attaaaaatg tcggacctat ttttgatgag gtagcaaact tagtacgcaa agaagaactg
    14221 cactcattag cttttgagga aacaacagtc tcatttttag aatattcggt tttagaagaa
    14281 atcatcgatg cggaattagt acctgtttca cagatgatcg aagaactcag agaagtgaaa
    14341 gatgaagaag aaatcgctat tatcgaaaaa gcatgtcata ttgcggatat ggcatatgat
    14401 cacatcctga aaatgatcca accgggcatg actgagattg aagtcgcaaa tcaactagac
    14461 ttttatatgc gttctcttgg cgcttcaggt gtttcttttg atacgatcgt tgctagcgga
    14521 ttgcgttcgg ctatgccgca tggtgtagca agcaaaaaaa tcatcgaaca aggtgacttg
    14581 atcacattgg actttggttg ctattacgaa gggtacgttt ccgatatgac acgtacattc
    14641 gctattggtg atcctgggga aaaattaaaa gaaatttatc aaatagtctt agatgctcaa
    14701 ttagcagtta ttgatgctgc aaaaccagga atgactggtg tccaattaga tgcagttgct
    14761 agagattata tttcaaaaca tggttacggg gaagcatttg gacattctac aggacatggg
    14821 atcggtttag agatccacga aggaccaaac gtttcttctc gtgcagaaaa gcaatttgta
    14881 ccaggaaatg tgatcactga tgaaccaggg atctacttac caggaatcgg tggagtacgt
    14941 attgaggatg acttgttgat cacagaagat ggaaatcgtg tgttgactcg ttcaccaaaa
    15001 gaattgatca tcttatagtt tcatcaatag aagaagtaag cgtaaaaatc agtgttcaca
    15061 gactttgtta tagaaaatca ctcgatttaa tgatacacta tagaagaact tttaagctgt
    15121 aagagataat aaagttgggt gttttctcca attcatagca gtttgcagct gatcggcggc
    15181 agttttctta ccacgtgatc actatgccgc atcatagaga attcggatgt atctcgtgct
    15241 taggcaaagg gaggaataaa taaatggcag atgaaaaaaa tttgatttta aacgcaaacc
    15301 aagatttagg agagatcgtg atcgctcctg aagtcatcga agtaattatc gggattgcag
    15361 cttctaaagt agaaggcgta tacggaatgc gcggaacttt tgccagcaat gtcactgaat
    15421 tattaggacg tgcagctcac ggaaaaggtg tttacttagc aaacgaggaa gatggcttga
    15481 aagtagatct ttactgttat ctagaatatg gtgtttcagt tcctaaagta gcaatggaaa
    15541 tgcaggaacg ggtcagccag caagtccttt tcatgacgga tatcgattta tctgaagtca
    15601 atatccatgt agtagctgtt gttcctgaaa aattagaaca accaacaatc gaagaacttt
    15661 ttgaagacga agaggaagag aatgaataaa gaattatcaa gacacgagat tcgggaaatg
    15721 gcattgcagg ctttatttcc attagacttc aatgcagacc taacaaaaga agatgcgatt
    15781 ttcaacgcaa tcgaattaga ccaccgcgac atgatcaacg aagatgaatc agagtttgtt
    15841 cctgtttatc ttgatacgct agttggcggt gtctgtgcaa aaaaagatga attggataaa
    15901 gtgattgaaa aacatttaaa aaacaattgg cacatcaatc ggatctcaaa aatggatttg
    15961 gtgatcttgc gtatcgcgat ttttgaaatg acttatgtga cagatgtacc agcggctgtt
    16021 gctttaaatg aagcaatcga gttggcaaaa acatttagtg atgaccgttc acgtaaattc
    16081 gtcaacggtg ttttatccaa tgtattgaaa gaattagaag ctggggcgta agtcctagct
    16141 tttttctatg aatcatcata aacctgttat aaatcgggaa aattctatgg tagaatgaaa
    16201 acacaaaaaa tatctgggag tggttagatg gcagaattga ttaacggaaa agaattagct
    16261 gaaaaaatgc aggcagagat tgctgaaaaa atccaaaaat tgaaagataa tggtatccat
    16321 cccggattag tcgttttatt ggtaggggaa aatccagcca gccaaatcta tgtaagaaat
    16381 aaagaacgtg tagcaaaaga aatcggaatc cattcattgg tagagcgtta tcctgagacg
    16441 atttctgaag acgaactact tgcagaaatt gaaaaataca atcaagatcc tagttttcat
    16501 ggtatattgg tccaattacc attgccaaag catatagatg aagaaaagat cctgttagct
    16561 atccgccctg aaaaagacgt agacggtttt catccgatga atttaggcag actccttgcg
    16621 ggtaaaccgg acaagattcc ttgtactcca tacggcatca tgaaaatgtt tgaagcttat
    16681 tctatcgatc cggcgggtaa acgagcagta gttatcggac gaagcaatat cgtcggtaaa
    16741 ccgatggctc agctactctt gatggcagat gcaactgtta ctgttgctca ttctaaaacc
    16801 gaaaatctgg ctgaactggc acaagaagca gatattttag tcgtagcaat aggacgtgga
    16861 cattttgtca caaaagaatt cgtaaaacca ggtgctgtag tgatagatgt agggatgaat
    16921 cgtgatgcta acggtaagct gatcggtgat gtgaaatttg atgaagtgga gcctattgca
    16981 ggctggatca ctcctgtgcc aaagggtgta gggccaatga ctatcacgat gctgatgtat
    17041 caaaccgtaa aatctgccga agcttttgga gcaggtgaat agatggcaca agaatattta
    17101 actgtcacta cattaacgaa gtatttgaaa agaaaatttg atgccgatcc ttatttagaa
    17161 cgcgtatatt taacaggcga aatatcgaat ttccgtttac gtgccaatgc ccatcaatat
    17221 ttcagcttaa aagatgacca tgcaaaaata tctgctatta tgttcaaagg agcattccaa
    17281 aaattacgat tccagccaaa agaaggaatg aaagtccttg tcgttgggcg tatttcatta
    17341 tatgaagcgg gaggttctta tcaaatctat gtagagcaca tggagcctga tggaataggt
    17401 gctttatatc aagaattgga agaacgaaaa gaaaagctga tgaaggaagg cctttttcaa
    17461 gggcctaaaa aaatgctgcc acgtttccca aagcgaatcg cggtccttac cagtccaagc
    17521 ggagcggtta tccgcgatat catcacgacg gtgaaaagaa gatatccaat tgtccaattg
    17581 gttttgttcc caacagttgt acaaggaaac caagcagcgg acaatgttgt caggaatatc
    17641 cagagagtag aagagatggg caatttcgat acaatgatca tcggtcgtgg gggtggttcg
    17701 attgaggatt tatggccgtt taatgaagag cgggttgctc gggcgattta tcaagctaat
    17761 acgccggtga tttcatctgt aggacatgaa acagatacca cgatcgctga tttagtagca
    17821 gatgttcggg ctgccactcc aacagcagct gcagaattag ctgttcctgt tttgtcagaa
    17881 gagttgatgc gcattcaaga acggcaagct agattgcagc aggcgtttgt acgccagatc
    17941 cagcgcaaac aagaacggtt cgagcgtgct cgacgttcct atgtatttcg gcagcctgaa
    18001 agattgtatg aaggtcagac catcaaatta gaccagttga aacagcgatt atatcaagct
    18061 acacagcaaa tttatcatgc gaaagaaaaa caaacgatcg cattaagcca ccaattggaa
    18121 caagtggctc cgctttatcg agttaaagca gctcgccaag aaactgctta tctagaaaaa
    18181 cgcttagaag agaaaatcca acaatatatg aaacaacagc agcagcgttt tgctcaagct
    18241 gttcaatctc ttgatttgtt gagtccattg aaaatcatgg gaagaggcta cagctatacg
    18301 acgttaaatg atcaagtagt aaaaagtgtc accgagattc atcaagggga tttgctgacg
    18361 atccattatc aagatggaat cgttaaagga caagtacaag agatagacaa aggagaataa
    18421 aaatatggaa aatccaactt ttgaagagtc actgcaagaa ttagaaaaaa ttgtcatgca
    18481 gttagaacaa ggagacgttc cattagaatc agctctagat tcgtttaaac gaggaatgga
    18541 attaagcaaa cattgccaag atacattagc taaagcagaa gagaccttga cgaaaatgat
    18601 gacagaatca aatgaagaag ttgattttga cggaaacgag gaaagctgag atgagattaa
    18661 ctgacttttc agctattcat ctacctgctg tagaaaaaga aatcttgtct tttttagatg
    18721 agcatacaac ggatagagga ctctatgatg cgatgagtta ttcagtgaaa gcaggcggga
    18781 aaagaatacg tcccttatta cttttaacgg ccgttgcttc ctttgatgag cctattgatg
    18841 ttcccgtcta tcaagtagca gctgctttag aaatggtgca tacgtattcc ttgatccacg
    18901 atgatttgcc tgcgatggac aatgatgatc ttcgtagagg aaaacctacc aatcataaag
    18961 tattcggcga agcattagct atattggccg gagatggttt gcttacagga gcattccagc
    19021 taatcagtat ggcacactta ggtaattctc ctaagcttct gctgcttcag caattagctg
    19081 tttgtgcggg atcacaaggt atggttgctg ggcaagctgc agatatcgag ggcgaatcga
    19141 aaaagctttc tttagaagaa ttagcattta ttcatgaaag aaagacaggg catttgattc
    19201 gttatgcctt gctggctggt ggcatattag cgaagcaacc tgaagaaatc ttattgctac
    19261 ttcaacgatt agccgaacat ttaggtttag cttttcagat aagagatgat ctactcgatg
    19321 tgattggtac aacgaaaaca ttagggaaaa cagctggtaa agatgaacgt atggaaaaaa
    19381 atacttaccc gcgtttatta ggcctagaaa agacacgtga ggcgttggag attgagctgt
    19441 tatccgccaa caagatcata gacaaattag aagaggacgt tttttctttt gatggcgggc
    19501 tgatgcgcca gatgatcaag caatttgatg tggagaaatg agggaaagaa tggaaaaaga
    19561 acgcgtagat gtattagcgg tcaaacaagg attatttgaa acaagagaac aagccaaacg
    19621 ttcagtgatg gctggtttga tttacaacga aaaaaacgag cggtttgata aaccgggaga
    19681 aaaaattcca gtgagtagtg aattaagagt caaaggtaaa aaacttcctt atgtttctcg
    19741 agggggatta aaattagaaa aagcactcaa agcatttgat ctatctgttg aaggaaaaac
    19801 attgttagat attggtgcat ccactggcgg atttactgat gcagctttgc aaaatggcgc
    19861 aaaaatgagt tatgctttag acgttggcta caatcagcta gcttggaaaa taagacaaga
    19921 tcctagagtc gtcgtaatgg aaagagtgaa ttttcgttat gcaaaacccg aagactttac
    19981 cttaggaatt ccagaagttg ctgttatcga tgtttctttt atttcactaa aactgatgct
    20041 gcctccatta catgctattt taaaagaaga gggagaagtg attgctttaa tcaaacccca
    20101 attcgaagca ggcagagaag ctgtagggaa aaacggaatt gttcgtgacc ctcagaccca
    20161 tcaaaaagtt ttagaagaca ttttgtcatt tgcggcacaa ggtggatatg atgtgctgga
    20221 attaagttat tctccgatca cggggggaga aggaaatatc gagttcttgg ctcacttgag
    20281 gaaagtaccc gaaagcggaa caatcaattc agctattaat atggcagaag tggtttcaaa
    20341 tgcacacgaa cagtttgacc ataaataaat tgtaactgtg aaaatcaagt gagaagtatg
    20401 cggattcttc tttattatac caaggaaata agctatcatt gattaagaag aatatgctaa
    20461 ggagcgaaga cgtaatgaga aaaaaagatc ggcatcgttt gattactcga ttgttgagtg
    20521 agcaagatat ccgaaagcag gaagaatttg ttgaaatcct gaaaagcaaa ggaatatctg
    20581 ttactcaagc tacgatttcc agagatataa aggaactgaa actgatcaag gtaccggctt
    20641 cagatggcgg ctaccgttat agcataccag ctgaaacaaa cgaagatgta aatactaaat
    20701 tggaaaaact attaaaagac gctttcgtgg cagttgacca aatggaaaaa tttgttattt
    20761 tgaaaacatt gccgggaaat gcttcggcag cagcaaattt gattgataaa cgttacaaga
    20821 aagaattatt ttcgattatc aatgatgacg ataatgtttt gatgattaca cgaactgaag
    20881 accaggcagt tttattgaag aaagacttcc tgcattacct ttaagttgag gagcgaatgt
    20941 gatgctacaa gaaatcagta ttactaactt tgcgattatt ccggaattgc ggctttcttt
    21001 tcacgaagga atgaccgcgc tgaccggaga gactggtgcg ggtaaatcca ttattatcga
    21061 tgctttaggt ctgttagcag gaggaagagg ttcaagcgat tatatacgtc aaggagcaga
    21121 aaaatgtgtg cttgaagggt tgtttgaact accaaaacaa gaaggattct ctgaactgat
    21181 ggttgaatta gggatcgaaa cagatgagga caatctgatt gttcgacggg atatgtcctt
    21241 aactggaaaa aatgtttgtc gagtgaatgg tcatattatc acattagcta atctgagaag
    21301 aatcggcagt tatttagtag atatccaagg acaaaacgaa catcaggaac tgctccagcc
    21361 tgaatcacat cttgctttat tggatcgttt tggagatgta gctttccaac agaagaaaaa
    21421 gagttatcag caagaatata tctcttatcg tgagctggaa aaaagggtac gaaaagtgca
    21481 gcaaaatgaa aaatcctatg ttcagagaat agatatgctc catttccagc aagaagaaat
    21541 agctgctgct gatttgcaag tcggagaaga agagaaatta aaagaagaaa gagaaaaact
    21601 tagcaattat caaaaaattg ctgatggatt agctgcaggt tacggagcac tgacgaataa
    21661 tgaacaaaac agtgtagatg gtgttggctt ggccgtttca gaactgcaag ggatcgccca
    21721 tctagatgtg gagtatgaag caatttatga aaatatccaa agtgcttact atttgctgca
    21781 agatgcaatc ggcgatatga gtcgtcagat tgatttattg gagttagatg agagtcgttt
    21841 agaagaagtt acgcaacgtt tagaattaat ccgacagttg aaacgtaaat acggcgaatc
    21901 cattgagtca atattggctt actatgacga aatcaccgaa gagctagctt cttctgattt
    21961 ttcagaaggt caattggaca aaatgaaaga ggaactagag caaaaagagc ttctacttca
    22021 gcaacaagcc gcagatcttc atgaagcgcg taaaaaaatc gcaaaagaat tggaaaaatc
    22081 cattctgcat gaattgaaaa gtctgtatat ggaaaacact gagtttgagg tacgtttctt
    22141 aaaagaagaa aatagacaat tgaatcatga tggatttgac caaatcgaat tttacattac
    22201 gaccaatcca ggagagccac tgaagccttt ggtgaaagtc gcttctggtg gcgaactgtc
    22261 tcggatgttg cttgctttga aaactatttt ttcttctgag caaggtgtga caagtattat
    22321 ttttgatgaa gtggatacag gtgtgagcgg aagagttgca caggccatcg cagataaaat
    22381 ttctcaaata tcgaaatact ctcaagtttt gtgcatcact catctgcctc aagttgctgc
    22441 agtcgccgac tatcaatatt atattgtgaa agcagtgatt gatggacgta cgcaaacttc
    22501 tgtttcagag ttgaaaacaa aagaaagaga agaagaaatt gcacgtatgc tggctggaag
    22561 tgagattaca aaactcacac tcgaacatgc aaaagaacta ttgaaactcg caaaaaggac
    22621 ctgagccacc tcgcagtggt tcaggtcctt ttcagcagct gatagctata ttttattttt
    22681 acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc agcattgtcc
    22741 aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca cgcattgttt
    22801 tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt tccacaagag
    22861 ggctttttta taaaaacttc ccttttttta aataataggt agttacgata caaataaata
    22921 atgttaatag aactagccat ataaaggcat catccctttc agcaataggt aacttaatgt
    22981 tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt aaagaagtta
    23041 aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc tgattcattt
    23101 gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt tccaccaata
    23161 catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt tgcgctagtt
    23221 ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg cttttttgga
    23281 tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg gcttccaaag
    23341 tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg acctgccaaa
    23401 tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag gtctggctaa
    23461 agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc tttttgggcg
    23521 tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga cttgttttag
    23581 caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga ttgaattctt
    23641 ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac ggaatttcaa
    23701 attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa taccagatcg
    23761 tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta aaatatttga
    23821 tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa ttggaataca
    23881 tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa tgaaacagtt
    23941 gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac gttcatgggc
    24001 acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt gtttggttgc
    24061 caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc ccctgatttg
    24121 gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg gacaatttct
    24181 atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc tgttttccaa
    24241 gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg gcgtttggtt
    24301 tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact ttcttaccta
    24361 tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc ttgattttta
    24421 aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg tggtagaaag
    24481 tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt caatgttgat
    24541 gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct caaaacttcg
    24601 tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc ttttcggcta
    24661 tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt tggctaaaaa
    24721 agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg aacttgataa
    24781 acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga caaaaggttg
    24841 cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat ggcaagattt
    24901 ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg atttcggact
    24961 ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac agtcatgttg
    25021 aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga ctgtacactc
    25081 ggcggtgccg gacatagtga atatttatta agacaactaa gttctaaagg gcatctgtat
    25141 gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc ttcttatgtc
    25201 gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga acgcttgagt
    25261 gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc tcctcagcta
    25321 gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat gcgcatggat
    25381 cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca tgaattagta
    25441 aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg tgaaatcgaa
    25501 cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat cattaaacaa
    25561 gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat ttttcaagct
    25621 gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga acaggcaatc
    25681 cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt ggaagacaga
    25741 attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc gggacttcct
    25801 gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc aatccttcct
    25861 tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg tattgcagaa
    25921 aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc aagattacga
    25981 atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt cttcttcgaa
    26041 tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt ctgctttgga
    26101 aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt taacagttat
    26161 gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa cagaaatcaa
    26221 tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt ctcgtactga
    26281 acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca atttaaggaa
    26341 agtgaagtaa atgagtctta aaaataaaat ttggcggttt atggaaaaga aaaatctaaa
    26401 tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta ttggattgtt
    26461 ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg ctggtacctc
    26521 attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag caaaaagagg
    26581 aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt attcaattaa
    26641 agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg aagaaaagaa
    26701 ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa aagatgcact
    26761 gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg gtagctatgg
    26821 gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa aagaaggtgt
    26881 cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg tcttttcttc
    26941 tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg gtaaactagg
    27001 tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta tttatcaaaa
    27061 agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag ctgtcgacgg
    27121 acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa cactgatgga
    27181 tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga aagcaaaaac
    27241 cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa tggaaggatt
    27301 gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg aaccaggatc
    27361 aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca acgagaatga
    27421 aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc atgactttgg
    27481 tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg tcgggatggt
    27541 catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac tgggatttgg
    27601 acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta cattaaatat
    27661 tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt tccaaatgat
    27721 gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat atatcagcaa
    27781 agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc tagggcaacc
    27841 tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg tcgaaagcga
    27901 aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat cggcaaaaac
    27961 cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata cagcctacct
    28021 gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt atctaacaat
    28081 gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc cattgatgaa
    28141 acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa aaacagaaaa
    28201 agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg atgctcaaaa
    28261 aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac aatctactgc
    28321 caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg gtgataagct
    28381 gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga aaatcctcgg
    28441 tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg ctccatatga
    28501 aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga aataggagag
    28561 aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt gcaatgacaa
    28621 ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt caggcgattc
    28681 gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg ggcggcttag
    28741 tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa aaacagctaa
    28801 ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc ggctttcttg
    28861 atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag cagaaattga
    28921 ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa ggttatccag
    28981 gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt attttcgctc
    29041 tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc gatggattag
    29101 ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg catcagcatc
    29161 agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt ttcttcgtct
    29221 acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg ttgggcggac
    29281 ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg attggtttga
    29341 tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag cttacaggaa
    29401 aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga tggtcggagt
    29461 ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc acattatgga
    29521 tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa taagaaagtg
    29581 cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca tgaattgggc
    29641 gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc acaagagctg
    29701 ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt ggatgaagaa
    29761 ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt agcaaaagcc
    29821 caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt agcggaatgc
    29881 ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat gacaggacta
    29941 ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat tgggtatcca
    30001 gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga attgtctagc
    30061 ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac caatatctat
    30121 gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa atggaacctt
    30181 caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc agaactacaa
    30241 gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga agtgttggaa
    30301 gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat aatggaaata
    30361 tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc tatttctgta
    30421 gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt tttccatggc
    30481 gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta taatgattca
    30541 aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac gtctaaggtc
    30601 gttttattgg ctggcggatt agacagagga aatacatttg atgaattaat tccttcatta
    30661 aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat ggatgctgga
    30721 aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc agttccttta
    30781 gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa tgccagctgg
    30841 gatcaatacc caaattttga aactagagga aacagattca tggaagctgt gaaccgatta
    30901 aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag gcggaggaac
    30961 agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc aagaaccgaa
    31021 tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg tccctgatac
    31081 aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt ccatgcataa
    31141 catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa tcctcagtga
    31201 ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg tagtgtacgc
    31261 tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac caggtgttac
    31321 caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag atgcggcttc
    31381 cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag aagtagcaga
    31441 tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga aaacagtctt
    31501 gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag tttttttaag
    31561 tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat attatgaaga
    31621 gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt atatcaaaaa
    31681 tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg caacttcgat
    31741 cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat atgttacgaa
    31801 tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa aaatgattgc
    31861 agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca tgggtgatga
    31921 agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg atgcttcaaa
    31981 acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt cttagtgagt
    32041 aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa tttgactcct
    32101 tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca agagccaaaa
    32161 aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa aacggttcaa
    32221 gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga agagatcaag
    32281 caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc caatttaaaa
    32341 agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt attgggtata
    32401 ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc agttgcagta
    32461 tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt gtcattagga
    32521 gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct taaaaaagca
    32581 cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt cgatttggat
    32641 gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta cccagtgatt
    32701 gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct ccctatcttg
    32761 gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa tcagttatca
    32821 tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag caataaaaat
    32881 ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa taatcttgcg
    32941 aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg tgtgatcgac
    33001 atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga agaatcgggc
    33061 caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga atcaagtgaa
    33121 gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg atcatctgat
    33181 tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac tccgtcaagt
    33241 ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta aaaaccagta
    33301 atttatggta gaattggaag cagtgaatat atctattaat aagtatattt taaaacggac
    33361 gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc atggcaaaaa
    33421 caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc gtggctgaat
    33481 atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa gggattaacc
    33541 gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca gtacgacaag
    33601 cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca gcaaacatgc
    33661 ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag gaaatcaccg
    33721 atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct cctgaacgtc
    33781 aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga atcaaagatc
    33841 ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca ggacctaaaa
    33901 cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc aacgaaatgg
    33961 tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa gattttggta
    34021 cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat gataagcaat
    34081 tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat atctctattg
    34141 tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg gatgcatacc
    34201 cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag tcagaaccag
    34261 tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa cagattttca
    34321 acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt ggcgtcgtat
    34381 tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa atcttcggcg
    34441 taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta tttacaaatg
    34501 taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg gcaaaaattg
    34561 ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa acaacaaaca
    34621 catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa ccaaaagagt
    34681 ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt gattaattca
    34741 tcctgggagg aataagatat ggaattctca attgataata atatcaatga cggcgcagta
    34801 atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat gattgaagaa
    34861 aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt aaaaaattca
    34921 aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg tgctggttct
    34981 cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg cgaagcttta
    35041 gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg tacaggtgct
    35101 gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg tgtcgtgaca
    35161 cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg tatcgctcgt
    35221 ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt agaagttgta
    35281 gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt acgacaaggt
    35341 gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga ctttgctgac
    35401 gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt ggctagtgga
    35461 gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct tgaaacatct
    35521 attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat gacattgttc
    35581 gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt aaatatcatt
    35641 ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt catcgcaaca
    35701 ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca agcacaaatg
    35761 caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac ttcttcagaa
    35821 gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg tcctagagtt
    35881 gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa ccgcgaagaa
    35941 acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg taaaaaataa
    36001 aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag aaattcagga
    36061 ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg taacaaaatc
    36121 tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt gtgcagaaaa
    36181 ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg aaataaagtg
    36241 gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg aaattgatta
    36301 ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt tagagaaaga
    36361 attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg gaatttcacc
    36421 agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca aggttgttgg
    36481 gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag tgtttggtac
    36541 gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac catgtagtga
    36601 attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg caacatttat
    36661 ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg ccgcttttaa
    36721 acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac gaataccaag
    36781 attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca acacagaatc
    36841 aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg gcgacttcac
    36901 gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt gaactacatc
    36961 aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct acacaaccta
    37021 aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct gaagctatgg
    37081 cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat ttagtagaag
    37141 aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca ctagatggag
    37201 atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg gagattgaca
    37261 gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag gaggaactac
    37321 gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt tctatagcat
    37381 tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat cagggctagg
    37441 acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc tgcctttgag
    37501 gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact tagccgtaca
    37561 aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat tgaagcaaca
    37621 aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg tataccagca
    37681 ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg aacaggttca
    37741 aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct atattttaga
    37801 aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat atgaacaggc
    37861 agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg aagattttga
    37921 aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc atgggaaaat
    37981 ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata tcatctcgga
    38041 cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg ctgcacaagt
    38101 aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag aaatgattat
    38161 gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac tagacagtgt
    38221 gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg aagctggtaa
    38281 agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac tattagatat
    38341 tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga agacgaaaaa
    38401 agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg tggtttactc
    38461 atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat gcggggatat
    38521 aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga ggaattgact
    38581 caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact tgagtacttc
    38641 aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac ggctgataaa
    38701 gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca aaacaaagca
    38761 gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga tgcagaagaa
    38821 aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc gacagaaact
    38881 gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat gcttgagtct
    38941 caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt ctccagctac
    39001 gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga caatgaaaat
    39061 gatactgatg taaactcaga cacaggagtc atagaagcac ctatgacggc atttgataca
    39121 caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa cagaaaagca
    39181 ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac tgcatggtga
    39241 aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc ggctgacctc
    39301 gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt ggtaccacgg
    39361 catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa taaaggagtg
    39421 tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt ccctatgcgt
    39481 ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa tcgaatctac
    39541 caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga tggacctcca
    39601 tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa agacattatc
    39661 attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg ggatacacat
    39721 ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga aatgtccatg
    39781 gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa gcaacgagca
    39841 gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac attagatcct
    39901 tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag ctatatttac
    39961 aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc ggaagctgaa
    40021 attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt caaagacggc
    40081 aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc atggacattg
    40141 ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt ggaagccgat
    40201 ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga aatcggttgg
    40261 gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat gactgcaagt
    40321 catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac tttagacgct
    40381 ggtactggat tagtccatac tgctccagga catggggaag atgactatat cgctggtagc
    40441 aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac agaagaagca
    40501 cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga tctattaaaa
    40561 gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca cgattggcgg
    40621 acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat cgataaattc
    40681 cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg gggtaaaaca
    40741 cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca gcgtgcatgg
    40801 ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac accagaaaca
    40861 atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt tgaaaaagaa
    40921 gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg cgaatttaca
    40981 aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga agctgtttta
    41041 cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc agaccaatat
    41101 cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt agcaccatac
    41161 aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat gagtaaatct
    41221 ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga tatccttcgc
    41281 ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga tatcttaagt
    41341 caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt agcgaataca
    41401 gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc tgtagacaaa
    41461 tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg gtacgacaaa
    41521 tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga tctatcgtca
    41581 ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga ttatcaacgt
    41641 cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt attgactcct
    41701 atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga ggagtacgtt
    41761 cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt gatggatacc
    41821 tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga agcaagaaac
    41881 agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa acaacaagtt
    41941 cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt ttcaccagac
    42001 tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt atttgatgat
    42061 gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg ccaagttcgt
    42121 aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc aaaaatcgtt
    42181 gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa gggaaaaggg
    42241 ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt ttccacgttc
    42301 acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa gtagttcgaa
    42361 agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt gtgtttggtc
    42421 ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt cccaatgaga
    42481 gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag cttctgggct
    42541 attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg tttcaaatcc
    42601 ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct gaatatagat
    42661 cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag atgttttgaa
    42721 tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt cagtcaaacg
    42781 tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga tagtgaattt
    42841 gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt cgcgatagcc
    42901 cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga agttttctaa
    42961 cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt ctgtacggat
    43021 ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa cctgtaaaat
    43081 atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc cgcgatcttc
    43141 tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat tgttccattg
    43201 agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca tttcttttcc
    43261 taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga tttgctggtt
    43321 caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca aacgattgaa
    43381 gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc caaaaaattg
    43441 aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc catctaatcg
    43501 gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat gggattgata
    43561 gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg cacggattgt
    43621 ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg ttccgattac
    43681 agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg ggtacaattt
    43741 acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt tttcattaga
    43801 catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc cttaaagctg
    43861 acggggattt tctttctatc attttggata gtgatcggtc cttcataagc tgcgatgtca
    43921 acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc caacaattct
    43981 ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga ctccaatgtt
    44041 tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc atgaggacaa
    44101 tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga agtcacatgt
    44161 tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg ttcaactaaa
    44221 aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag tccgcttgca
    44281 gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac aagtttagaa
    44341 atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg tttattgttg
    44401 actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc tcgatttggt
    44461 gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta acaaaaaaaa
    44521 cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt ttacaacaaa
    44581 aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat atatacattg
    44641 ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc gtcataaaga
    44701 gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac gactaaacga
    44761 tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa aaacgtgtaa
    44821 tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca tgtaccttga
    44881 tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta aataaaactt
    44941 gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac caaatcaccc
    45001 ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt gtaccgaata
    45061 aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct acaaattatg
    45121 ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag tggaaatagc
    45181 tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag acggagaaat
    45241 aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac caggcgctga
    45301 agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc tgattgctgt
    45361 aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc tggcaattga
    45421 tggtatttta gacggcacga tccttttacc aggattaact ggtccattta cggtatacaa
    45481 gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg tggtcggaca
    45541 aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg gtgcttttcc
    45601 tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg ccgcacaaga
    45661 tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc tggcaacgat
    45721 ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc aaagcggagt
    45781 tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca agcaggccat
    45841 tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag gtaaaataat
    45901 gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc aagcagcttt
    45961 aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa aagcacagga
    46021 atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga cttttttcaa
    46081 tattactcat atggacacca tttatattgc ttctccgaat agtcttcact ttagtcaagc
    46141 taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt tttcgacgcc
    46201 aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt tttttgaagc
    46261 agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac cattaaaaga
    46321 cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg accaagtatt
    46381 agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat tgatggattt
    46441 aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag aagtccatta
    46501 tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct tacgttatga
    46561 gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac cgtcagagat
    46621 ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac aagcacaatt
    46681 tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag aaaatccaat
    46741 ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc cagcttgggg
    46801 attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga tttatgattt
    46861 aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt aatgaacgaa
    46921 tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg gtttatctca
    46981 ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag tatagtagcg
    47041 gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact actaaacgtg
    47101 gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact agctattcaa
    47161 gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca atctttggtg
    47221 ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga aatcttgatt
    47281 ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc acatcaagtg
    47341 aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag ccaaatcatt
    47401 gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc agcgacagca
    47461 gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac aattgacgtg
    47521 acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt accgatacga
    47581 aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg tctgattttc
    47641 tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag aggtgtaccg
    47701 gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct agaacaattc
    47761 aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg tttagatatt
    47821 acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc ttatttgcat
    47881 cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact cgtacaagat
    47941 aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact tgaagagatt
    48001 tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc agctcctgca
    48061 gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga atataacaaa
    48121 gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa aggagcaagg
    48181 cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt ggttgccaga
    48241 ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca gaaaaccaac
    48301 aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc aatgcaagcc
    48361 gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc cgaccagtcg
    48421 tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac tcttctacgc
    48481 aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc atacagcagg
    48541 caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt ggagaaactg
    48601 gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat tcagagatgc
    48661 tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa aatccgcaac
    48721 tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca gctcaagata
    48781 gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag atccaccgtt
    48841 taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt taccgtccgc
    48901 ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg acgactgaat
    48961 cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc accttgccga
    49021 atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt atcaaacaag
    49081 aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt gccttcatgc
    49141 tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta gtctgtgcaa
    49201 aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga aaagaagaac
    49261 tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa cgtctcgacc
    49321 gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct gatgttgtct
    49381 ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa aaagtcactt
    49441 ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca aaagatgtgg
    49501 atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc aaagatcttt
    49561 tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa aaacggttag
    49621 accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa agtacattgg
    49681 atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta cggaaaaatt
    49741 ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa gaagaacaag
    49801 ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc aatctaggag
    49861 aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac ggcagacaga
    49921 ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta gggaaagctt
    49981 ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta caatatgtca
    50041 aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg ggatcattaa
    50101 acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct atcacgatga
    50161 caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga agcttagaag
    50221 atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag ttcaaagcgg
    50281 aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt actggtgaag
    50341 gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat aaggtagaac
    50401 tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat gccttattgg
    50461 aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa aatatcccat
    50521 ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag cgtatagctg
    50581 atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta agcgatgtct
    50641 tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc cagaccgatt
    50701 tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta gccttcatgt
    50761 tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct attttctcaa
    50821 aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta gaacgaaaat
    50881 atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt cttgaaaata
    50941 aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg tgtgcgcact
    51001 ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta ttggcatgct
    51061 tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc aaaaaaaaca
    51121 atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa aatgcaaaaa
    51181 gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc agatgcagat
    51241 aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt acgtttcatg
    51301 aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt catcaatatg
    51361 caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa tctaatgaaa
    51421 taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaat ctagaagcca
    51481 tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa gcgattgcgg
    51541 ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca gaagaatcac
    51601 ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa ggaaatcata
    51661 tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct attgcgttta
    51721 ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa taaatacaac
    51781 ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg ttataaggag
    51841 aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac tatcaagttg
    51901 gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac atttagttgc
    51961 tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag tcgctgtaat
    52021 gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg ctgacagttt
    52081 agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca atggttttgt
    52141 ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg ggtataatct
    52201 tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag ctaccttgat
    52261 tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg ttccttcaaa
    52321 tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg gcacactgtc
    52381 agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt attttacagt
    52441 tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg ccaacattac
    52501 gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag ccattttacc
    52561 agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc gtgtagctga
    52621 tgggcaactg atcattgatt tggtgcaaaa atatattgca gaaccattcc taggattatc
    52681 tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct ggttctttgg
    52741 tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac aagcacaact
    52801 aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac cagccgtgtt
    52861 agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg atgcttttgc
    52921 atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga ttttctctaa
    52981 acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct tcaatatcaa
    53041 cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta tcccattctt
    53101 gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg gcctagtcgc
    53161 tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat tcttagcaac
    53221 aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga ctttcttgat
    53281 ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg aacaacaata
    53341 agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat tttcgaggag
    53401 gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa gaattgttag
    53461 aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga atcaagctgt
    53521 tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag gaactaatca
    53581 aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag aaaatggttt
    53641 aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt attggttctt
    53701 tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat agtatggtca
    53761 caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa aaaagagaag
    53821 aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc cagcatcgtt
    53881 tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga ccggcaaatc
    53941 agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc aaaataaccg
    54001 ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc gtacatcgac
    54061 aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt cgacctgtga
    54121 ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga atgatatgct
    54181 tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc tagatatgat
    54241 cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa aatgaagtaa
    54301 gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac tagctttttt
    54361 gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc ttcgattgag
    54421 gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga aaggcgagaa
    54481 cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga ggaggatcgg
    54541 ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca agagtaccta
    54601 atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg cgggatcaat
    54661 cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt atcttatctg
    54721 gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt tgataaaatc
    54781 agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg aaaaataaag
    54841 cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca gatgccggaa
    54901 aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt caagcaaaaa
    54961 ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg atatatcgtt
    55021 tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga tcatgatgct
    55081 ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac atttgaatat
    55141 tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt tgcttctgta
    55201 atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt ggaaaattat
    55261 ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca ttatgcgaaa
    55321 tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct ctcaaaagta
    55381 gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca gctattatta
    55441 gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac aaaagaatat
    55501 gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga ctatttttga
    55561 taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat ttggcatttg
    55621 aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg ttttcttttg
    55681 ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt tcccgatttt
    55741 ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca ttcattggaa
    55801 aggacctccc tacattattt cattctacct ctagtttatc atctgttaag gactagtgga
    55861 aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa gtaaaaaaca
    55921 aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata gagcaatcgc
    55981 ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtataaata acccctgtat
    56041 cctttgcggt acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa ctagttatca
    56101 tttttatata aactatcgaa cacagtattg attttttctt tatcctgatc ttcaagctct
    56161 cgaacaatat gaagataagt agacattgta gtttctaaac tgctatgtcc taaacgtttc
    56221 gatacgctaa gtatatttac tccttgataa agtaaaatag atgcatgtgt atgccttagt
    56281 ccatgcaaag taaattgctt ttcaatccct aattctttta attttttgcg caaatatttg
    56341 ctgacggcgt ttgatgaaac taatccattt tttaagttga aaaaaacaaa attatgaggg
    56401 tttttgattt caaaattctc gaataattct ttttgattga ttttaaactt ttttaagaga
    56461 tcaatcgtat gtttatcaat ggaaattttc ctatttgaag tttcgttttt tgtttttccc
    56521 cattcattta atttataatt ccatgtttta ttaatagtga tgatttgatc ttcgaaatcg
    56581 atatcttccc atgttaatcc tagaagttca gcaaatcgca ttccagtggc accagcaact
    56641 aacacgagca taggagaaga gtactgcgca gaaaggtttt cttctacgag tttcattaaa
    56701 gatttaaatt gatcataatc taaatattta tcttcttttg gcttcaatga atcttttcct
    56761 ttgattactg cttttctagt tggatcaaac ggaattaatc cttcttctac ggcatctttc
    56821 aacgacgctc taatatggtt attaaactta ataacggtag attttacatg gtctttagca
    56881 tacttattta aaaagcgttg atatcccact ctatctaaat cagaaatcaa taccgctggc
    56941 atatatttct ttatgttcat taacgtatct tcgtattttc tataagtgat aggagatact
    57001 gttccttctt tataaagttg catccaatcc tcaaagtaat ctgataataa tagatttttg
    57061 cgatcccctt taaggccttt gttcaagtta tattctaatt cgttggcggc atcttttgct
    57121 tctccttttg tcttaaatcc tgattttctt atcttcgtgt attttccgtc atcttttttg
    57181 tatgaaattt cgtattgcca actatttcca cgtttgacta atcttgccat aattgaatca
    57241 gctctctttc tctgatacaa taggaactat aaagaagccc attgtttggg ttttattatt
    57301 ttttgcacat cctttttgct tgccggcgca aggatgtgct atttgtcgtt ttaatggtat
    57361 aattggatgt gtattagatc atccaatcag aggtgtttat tatgattaaa gtgattttta
    57421 aaagaataac tgaacttcca gagtttagat tatttgttga ttattggtat gtctggtttt
    57481 ttattgttgc attgctgata ggtctagatg tcatattaga aagaaaactt aagtaagagt
    57541 tgagttaata aattgaattc ctaaatgaga tattttgtag cccatatttt gtttgccgac
    57601 atcacctaat actatggagc taatcgtttt ttctaacaga ccaaaatcag ataatctatt
    57661 taagctacca tctattagct cttgtgaata gccagacatg ctgatatttc cagtgaaatt
    57721 ttcgtttcca cgactcatta agcatgctaa caattgaatc tcaatcgatg ttaattgatt
    57781 cagtacaaga agataatact cctcgttatc ccatgaagcg ttgttaattc gaagtaaaca
    57841 attcttataa agatttttat acatatctat tttattttgt gcctttgatt tttctacctc
    57901 gtcatttatc ccttcgaata ttccaatgag ttggtccttg ttctctaagt tgaaaacatc
    57961 ttctggaatt tgatcgcgaa tgttttctaa tgattcgttt aaatttttgt agaatttctc
    58021 gatgcgtttg aaacgcttct cgtttttaga cccaaaataa agtgactgca caacgccacc
    58081 aatataagga attgctccaa taccgccttc aactattaat tcaagatatt cttttttggt
    58141 tagtttttca ttgctattat ccatagttac ctcctatttt gtcatttcct ttacaagcat
    58201 tgtgactcct gatggcactc ttttgtatag gttttattat tgtgcacaat ctaactctta
    58261 gtggggcgtg gttcatttat tttttactta ctttactatt aatcatatcc cattgagcat
    58321 aggatttttc tatgttcgat cctcttgaac taatttctcc tgtaaaagtt actttatcgc
    58381 cctttgtaaa ggtatcagca ttgtttaagt ccttcgcaaa aattacatat gaaactttag
    58441 gtgttgcaga aatatcattc caacttttat catcgtactc tttgtctgct ataatggcta
    58501 ttctagtagt cattgattcg ataacagttc cttcaaaagt atatgtttta ccactaatat
    58561 atttagtaaa agctgtattt tgctcagtag ctggagtaat agaataataa ctatctaaga
    58621 aagcacccca gtcatgatta tttgtctcta aaaattcttc aaattgagtt aggcttttat
    58681 tgctgctgtt agatgtagta gtttgagtat cagatgatga gctttcctgt ttgttattgt
    58741 catttgcctc gtttgatgag gaaagcgaga aacctacggt agtaaaaata atcccgataa
    58801 taaggagaat cataggtgtt ttcttatttg tttttttgat aaatcttaca attaacatga
    58861 ttaaacctat aataaataaa acaataccag taaaaccaaa tataccgtcc attaatagat
    58921 cctctttctc ttttgatatt gaatattata aaccaagcaa ttcttttttc ttaaaatcga
    58981 attcttcttg attaataatc ccttcatcta acaactcttt atacttttta atttcatctg
    59041 cacttgaaat ttgtgtttct ttggaattat tatctaatat agattgttgc ttctctatat
    59101 atttttttaa attttccatt tcattccaat atttttttgt aaacataacg gtgttttcat
    59161 ctttcgtggc cgctaatata cctccgcgac tttcgtttcc gcctagcaag gtaaattgta
    59221 tataaccatt actcatacct ggttttttta attgtacggc tgatatattt ttaaagggga
    59281 tagttttttc tcctttaata ccttgattaa ctaaatttat aaatcctttt ctaataattg
    59341 aaatagaatc atcagttact ttaatcaagg tttttcctgg agattttatt aaaatctctt
    59401 tttccatttt ttcttcctca tttctgtgat atgatttttt ttgtagaagc tcatatatga
    59461 ggaaagagcc cgtgttgcag cacgggcttt tttaatgttt tgctgaaatc ggttttttta
    59521 ataattcttg atgtattcat acatattgcc ttgcgtaaga atattttttc ttaaaatagc
    59581 attggcagac agcataacaa gtgtatctga gcttattctg aatagaataa tactccataa
    59641 atttttcgag attaaactga gattcatcag tcagttcgtt ctcaatatag atatttaata
    59701 aaattagaat agctatttta tctgcttctg tttcaaattt tgagtgaaaa gttgtagaag
    59761 tatcgtacaa aactgaaaat tcaaaatgag aggcgctgaa atgtgcaagt tcatgagata
    59821 aatgaaaggc ttctgcagtt tcaccgtata gattttcatt caaaaaaatg attctgggtt
    59881 ttggatagta gaaaccaggc tctttcatct ccatataaac tactttcaaa ttgtattcgc
    59941 tcaacatttc tttcaatttc aaatacatac aaaccatcac tccaactatt cattttcctc
    60001 taaagcttta gcaattgcaa tcgctttacg cattgtctcc ttagatattt cttttccatc
    60061 aaaagaaaaa acagtatcgt cttctgataa atccacatgt ttaggggttt ctctttcttc
    60121 tctacctaga aggtagtcta cagagacatc gaaatagtca gcaatccgac ttaattcatc
    60181 tgaattagga gtattatttt tccactttgc tagataaccg ttagaatatc caaaattaat
    60241 ttctaactgc cgtatagata tctttttcct cttcgccaag tcttttatta tttcataggt
    60301 attcatcgat aaaacaacct ttctaaatgt ttacaaaaaa agtttagaaa aatacgcaga
    60361 aataatttga ctaattctga gtaatgagct atactatatc ttgtaaacag atttaacaac
    60421 taaaaagaca acaaaaaaca ctattgatat gtaaatgcag accgccaaga aagctttaaa
    60481 atcaatgttt ttatgtctta tttaattatg ctcttagtat agagtattac tcagatactg
    60541 tcaattgaat ttagaaaaaa gttgttaaat ttgtttacga atataaaaga aaggaatgag
    60601 aaatatgaac acaccgcaaa tttttaattt cgaacaaaac gaagttcgga caattttagt
    60661 aaatgatgaa ccatattttg taggcaaaga cgttgcgagt gttttgggtt attcaaacac
    60721 taaagatgct ttgtcgcggc atgtagattt ggaagataag atggggtcgc gaatcacgac
    60781 ctcaggtcaa tcaagagaga tgacaatcat caacgaatct ggtttgtaca gtttaatctt
    60841 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacaagtg aagtgttgcc
    60901 agcaattaga aaacatggag gttatctaac tccagaaaaa gtagaagaag ctttgcttaa
    60961 tccagataca atcattcaat tagcaactca actaaaagaa gaaagaactg gaagattaat
    61021 cgcagaacaa aagattgcag agtacgaacc taaaatctcc tatttagata gcatattatc
    61081 ttctacagat tcagtaacaa ttagtcagat tgcagcagat tatgggatgt ctccacaaca
    61141 gatgaataaa ttacttcata aactaggtat tcagaaaaaa gtcggtaacc aatggttatt
    61201 gtgcaaaaaa cacatgaacc aaggatacac aaaatctcat acaactgaga tcccgaaagc
    61261 cgatggtggc actaaaattg taatgaatac caaatggaca cagaaagggc gtctatttat
    61321 ctacgaatta ctaaaaaaag aaggatatta ccctcaaatg gatttagagg aaattggtta
    61381 gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc
    61441 aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga
    61501 attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac
    61561 agaattatag attcgattat cactatgtac gaattgtaaa gggaggaaca gctaatgcaa
    61621 tatctagaag caaaaatccc aattccagaa ggctatgtaa ttatctccca agtggattat
    61681 gaggagttaa aaaaagctga tgatactggt agatggatga cgttgccaga agtgctagaa
    61741 cggattaaca gaaaatatga ttggtttact tctagagttt taaagaaccc aagatataga
    61801 aatattatcg atatcgagaa aaacaaaaat ggatttgtct attatccagt tgaaggaaga
    61861 gacacatatc tatttttaag aagtaaaaca cttgaatttt tagaaacaaa tttttcggaa
    61921 atcttaagga ggtaagcgga tgggtaaatt taatagagca ctagtattca gcgcaccact
    61981 aattatctac gctttaggtc tttggggaag cagacaagca ttaatcggca caattgtata
    62041 tatggtctgg atctttatgg ggcttgatga agctgaatat agagcgaaaa agccagtcgg
    62101 gagggactga cttaacctaa ttaattaaaa attgtaacaa ataacgcaaa aataaagcta
    62161 atgaaattgt attgttagga tctactccaa tagttatatt tacaatttcg gttactacta
    62221 gtcctatcaa agctgcccca gtttgctcta aataccacat tttatctttg tagaaagcag
    62281 gtgaagcttg ttgtttttca gatggttcat tagttatatc tttgttcttg gctgtattta
    62341 tttcttcaaa aagctttata gaatcatcaa ggactttcct ttgctcatta aggatatcaa
    62401 aaaacttagc ctcaaactca tcagatggat tagaagtgtc tttaaaaagt agattttttg
    62461 tactaacttc cggttcggaa gaaaatgctt tttctaattc ttgggtagct acccctattc
    62521 tgaagtcgta cttagacatg tcaacggcaa atttttctgt gattttagag atatcagaaa
    62581 aataaggttt tggcatagta gctgccattt ttttagcaat attaaaactt ggagattcta
    62641 attgtttctg tattgcgaac aaagaaggag gaatcatatt agcaatagat ttgttacgtt
    62701 tctcgacaat ttgaatgaga gaatcattga gtattgcttg cgaagctata aaagtatctt
    62761 gaattgcaat taatttttta gcttgttcta tcgcaggctg catagcgtta acgacatcta
    62821 gagaaggagc aatggtttca ccgattaatt ttgaagaagc tatcaatttt tgataataag
    62881 gagtatctga aatatccaat tatatacacc acctttttac ctaaattata ccaaaaggga
    62941 gagaaaaaaa tgcaataatt agtaattttg aaaaataaag aagctgtgac tacgagctta
    63001 caagtcgcag aaacattccg aaaggaacat agagcagttt taaaggcgat tgacgatcta
    63061 aaagatgggc ttgcacaaaa gtatgcagac ctattttggg aagatgtgta catccatccg
    63121 caaaacaaac aggcttatcg aattgtctat atgaatcgtg atggctttac tcttgctgta
    63181 ttaggattca acaatactca aaaagtttta gagttcaaat tgaaatacat tgaagcgttc
    63241 aacgaaatgg aaaaggcaat tcgcaaaaat acagcttttg acacaattga agacatgatg
    63301 atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaaaatact
    63361 atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatac gaaagttatg
    63421 gaagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca
    63481 aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt
    63541 aagagattcg atgatgctgt ttcatttatt gaaatgtggt taccagaaac tgcgattcgc
    63601 atggaaatcg atcaactgaa cagacaacaa agactttttg gtgatgaaaa tgaatagagc
    63661 tgaagcgcta agaatcggga cggtaattgc taatcgctgg tggagacaca ataaaccaat
    63721 catcctaagc caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact
    63781 ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa
    63841 aaagaacttt ccactctaga tcaatatttg actgatccta gttggggcaa atcgaatatc
    63901 aaggaaacaa ataatcgaaa aatcagacga aatcttttga cggatgaaga actagcatgt
    63961 gatcaagacg atttgggaaa ttttgtgagt atttgggatc atgtctatct tatccattta
    64021 tcgaggaagt ccaaaaaacc tgaatatatc tatgtcatcg aagatggctt gattgatgcg
    64081 ctagaagagt atgacagaga taacttgatt gatatctctt attacggatc aggtaagaaa
    64141 tacattgctg aaatggaggc agaatttgat gagtgaaaac aaagggacaa cgacttttga
    64201 aaaacttttt agtcgcaagt taaataaaat actcaagaaa aaaggaaatt ttgattattt
    64261 atcttgggct cacgcatggg agattatgaa aaagaatgat ccacaggcaa cggtaactat
    64321 taatgagtat aaacactaca gagttgtttc tggaactcat caagactttc ttgttgagga
    64381 atataaacct tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa
    64441 aggacacacg gaaaccgaat tatttcctgt tttagattat cgaaaccaac cagttgttaa
    64501 accaaatgca atgcaaatca ataactcatt gaagcgatgc tttgtgaaag cattggctct
    64561 acacggactg ggattatatg tatttcaagg ggaagatatt ccaacaccac ctagaatcga
    64621 tacgaagaaa ttaaacatgc tagagacgat tctagaagct ttcaatgagc agatgggtaa
    64681 agatatgacc aaaaccttaa tcgaatatgt taatgagcag acagataaat tagggctctt
    64741 agctgataac gttgaaacta ttgaacagtt aagctatgag caatgtgcct tgatggagcg
    64801 agcaatagca gctaagagaa aagaattaga taagaagtga tatgagtgtt taaaccatta
    64861 atcgattcat attcagcggt tctgaaaaag ttcaaaggaa aagacatagg tgcaaccatc
    64921 aatgaggaag tgaacattga tcgactaaag acgatgtatg acggctacga tggtgatcga
    64981 gttattgaaa ttcgttttat tgatcctaga cgtttcacag tacagcaacg aaacttcatc
    65041 tatgcactca taggcgatat tttcatcgat acaggcatgc caacggactt ctggaaggaa
    65101 ttcttttact tccgctttga aggtgtcaca gggcgcaaaa taagcctcaa agatgaatcg
    65161 aatacgactg tgagtgatgc taacgtctta gcaaatatca tcttagattt catctttgaa
    65221 catcatattc ctttcaaaga aggctatgag attttaccag cgaatcaaga atattacttc
    65281 tacaaatgca tcacaaaaag agtctgttgc atctgtggca aaacaggagc tgatatcgat
    65341 cactttgaca aagcgctagg aagacgaaag cgcaaagaag ttgatcattc agagtacaca
    65401 tttgcagcac tctgcagaat ccatcacacg gagaaacaca agataggtgt gatcaatttc
    65461 aaaaataaat atcaaatcaa agggatcaag ttaaaccagg aaacaattaa gaaacttaga
    65521 ataggaggat aaaaaatatt ggctgacaac
//
LOCUS       DAPBCS010000008        59197 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPBCS010000008 DAPBCS010000000
VERSION     DAPBCS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 59197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.008"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(543..977)
                     /locus_tag="RD521_000660"
     CDS             complement(543..977)
                     /locus_tag="RD521_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770418.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(994..2199)
                     /locus_tag="RD521_000661"
     CDS             complement(994..2199)
                     /locus_tag="RD521_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDT7770419.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2893..3279
                     /locus_tag="RD521_000662"
     CDS             2893..3279
                     /locus_tag="RD521_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7770420.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            3373..3564
                     /locus_tag="RD521_000663"
     CDS             3373..3564
                     /locus_tag="RD521_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770421.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            4078..4410
                     /locus_tag="RD521_000664"
     CDS             4078..4410
                     /locus_tag="RD521_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7770422.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            4320..4514
                     /locus_tag="RD521_000665"
     CDS             4320..4514
                     /locus_tag="RD521_000665"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7770423.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            4706..4861
                     /locus_tag="RD521_000666"
     CDS             4706..4861
                     /locus_tag="RD521_000666"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDT7770424.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            5137..6477
                     /locus_tag="RD521_000667"
                     /pseudo
     CDS             5137..6477
                     /locus_tag="RD521_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6718..8334)
                     /locus_tag="RD521_000668"
     CDS             complement(6718..8334)
                     /locus_tag="RD521_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDT7770425.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            8452..8670
                     /locus_tag="RD521_000669"
     CDS             8452..8670
                     /locus_tag="RD521_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770426.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            8865..9326
                     /locus_tag="RD521_000670"
     CDS             8865..9326
                     /locus_tag="RD521_000670"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7770427.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(9519..9758)
                     /locus_tag="RD521_000671"
     CDS             complement(9519..9758)
                     /locus_tag="RD521_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770428.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(9782..11305)
                     /locus_tag="RD521_000672"
     CDS             complement(9782..11305)
                     /locus_tag="RD521_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDT7770429.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(11323..11445)
                     /locus_tag="RD521_000673"
     CDS             complement(11323..11445)
                     /locus_tag="RD521_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDT7770430.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(11475..12458)
                     /locus_tag="RD521_000674"
     CDS             complement(11475..12458)
                     /locus_tag="RD521_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7770431.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(12482..12631)
                     /gene="rpmG"
                     /locus_tag="RD521_000675"
     CDS             complement(12482..12631)
                     /gene="rpmG"
                     /locus_tag="RD521_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7770432.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(12652..13029)
                     /locus_tag="RD521_000676"
     CDS             complement(12652..13029)
                     /locus_tag="RD521_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770433.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(13061..13240)
                     /gene="rpmF"
                     /locus_tag="RD521_000677"
     CDS             complement(13061..13240)
                     /gene="rpmF"
                     /locus_tag="RD521_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDT7770434.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(13255..13524)
                     /gene="rpsN"
                     /locus_tag="RD521_000678"
     CDS             complement(13255..13524)
                     /gene="rpsN"
                     /locus_tag="RD521_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDT7770435.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(14047..15813)
                     /locus_tag="RD521_000679"
     CDS             complement(14047..15813)
                     /locus_tag="RD521_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770436.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(15773..17527)
                     /locus_tag="RD521_000680"
     CDS             complement(15773..17527)
                     /locus_tag="RD521_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770437.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(17637..18206)
                     /locus_tag="RD521_000681"
     CDS             complement(17637..18206)
                     /locus_tag="RD521_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDT7770438.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(18224..19639)
                     /locus_tag="RD521_000682"
     CDS             complement(18224..19639)
                     /locus_tag="RD521_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7770439.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(19650..20318)
                     /locus_tag="RD521_000683"
     CDS             complement(19650..20318)
                     /locus_tag="RD521_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDT7770440.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(20448..21032)
                     /locus_tag="RD521_000684"
     CDS             complement(20448..21032)
                     /locus_tag="RD521_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7770441.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(21363..21596)
                     /locus_tag="RD521_000685"
     CDS             complement(21363..21596)
                     /locus_tag="RD521_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDT7770442.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(21611..22561)
                     /locus_tag="RD521_000686"
     CDS             complement(21611..22561)
                     /locus_tag="RD521_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDT7770443.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(22558..23400)
                     /locus_tag="RD521_000687"
     CDS             complement(22558..23400)
                     /locus_tag="RD521_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDT7770444.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(23402..24121)
                     /locus_tag="RD521_000688"
     CDS             complement(23402..24121)
                     /locus_tag="RD521_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDT7770445.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            24813..24971
                     /locus_tag="RD521_000689"
     CDS             24813..24971
                     /locus_tag="RD521_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770446.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            25043..26269
                     /locus_tag="RD521_000690"
     CDS             25043..26269
                     /locus_tag="RD521_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7770447.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(26357..26749)
                     /gene="rpsI"
                     /locus_tag="RD521_000691"
     CDS             complement(26357..26749)
                     /gene="rpsI"
                     /locus_tag="RD521_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDT7770448.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(26765..27208)
                     /gene="rplM"
                     /locus_tag="RD521_000692"
     CDS             complement(26765..27208)
                     /gene="rplM"
                     /locus_tag="RD521_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDT7770449.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(27727..28194)
                     /locus_tag="RD521_000693"
     CDS             complement(27727..28194)
                     /locus_tag="RD521_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDT7770450.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDYPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(28314..29852)
                     /locus_tag="RD521_000694"
     CDS             complement(28314..29852)
                     /locus_tag="RD521_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDT7770451.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            29932..30618
                     /locus_tag="RD521_000695"
     CDS             29932..30618
                     /locus_tag="RD521_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDT7770452.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(30819..31346)
                     /gene="msrA"
                     /locus_tag="RD521_000696"
     CDS             complement(30819..31346)
                     /gene="msrA"
                     /locus_tag="RD521_000696"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7770453.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(31430..32107)
                     /gene="pgmB"
                     /locus_tag="RD521_000697"
     CDS             complement(31430..32107)
                     /gene="pgmB"
                     /locus_tag="RD521_000697"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDT7770454.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(32104..33234)
                     /locus_tag="RD521_000698"
     CDS             complement(32104..33234)
                     /locus_tag="RD521_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7770455.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(33234..35417)
                     /locus_tag="RD521_000699"
     CDS             complement(33234..35417)
                     /locus_tag="RD521_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDT7770456.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(35414..36439)
                     /locus_tag="RD521_000700"
     CDS             complement(35414..36439)
                     /locus_tag="RD521_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDT7770457.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(36484..37311)
                     /locus_tag="RD521_000701"
     CDS             complement(36484..37311)
                     /locus_tag="RD521_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7770458.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(37308..38375)
                     /locus_tag="RD521_000702"
     CDS             complement(37308..38375)
                     /locus_tag="RD521_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDT7770459.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(38401..39237)
                     /locus_tag="RD521_000703"
     CDS             complement(38401..39237)
                     /locus_tag="RD521_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7770460.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(39234..40103)
                     /locus_tag="RD521_000704"
     CDS             complement(39234..40103)
                     /locus_tag="RD521_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7770461.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(40173..41456)
                     /locus_tag="RD521_000705"
     CDS             complement(40173..41456)
                     /locus_tag="RD521_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDT7770462.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(41470..43155)
                     /locus_tag="RD521_000706"
     CDS             complement(41470..43155)
                     /locus_tag="RD521_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDT7770463.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            43430..44455
                     /locus_tag="RD521_000707"
     CDS             43430..44455
                     /locus_tag="RD521_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7770464.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(45050..48724)
                     /gene="rpoC"
                     /locus_tag="RD521_000708"
     CDS             complement(45050..48724)
                     /gene="rpoC"
                     /locus_tag="RD521_000708"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDT7770465.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(48782..52399)
                     /gene="rpoB"
                     /locus_tag="RD521_000709"
     CDS             complement(48782..52399)
                     /gene="rpoB"
                     /locus_tag="RD521_000709"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDT7770466.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            53127..54095
                     /locus_tag="RD521_000710"
     CDS             53127..54095
                     /locus_tag="RD521_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7770467.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            54113..55018
                     /locus_tag="RD521_000711"
     CDS             54113..55018
                     /locus_tag="RD521_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770468.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            55015..55821
                     /locus_tag="RD521_000712"
     CDS             55015..55821
                     /locus_tag="RD521_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7770469.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            55957..56946
                     /locus_tag="RD521_000713"
     CDS             55957..56946
                     /locus_tag="RD521_000713"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDT7770470.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(57263..57787)
                     /locus_tag="RD521_000714"
     CDS             complement(57263..57787)
                     /locus_tag="RD521_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDT7770471.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            58074..>59197
                     /gene="scm"
                     /locus_tag="RD521_000715"
     CDS             58074..>59197
                     /gene="scm"
                     /locus_tag="RD521_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDT7770472.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSA"
BASE COUNT        18027 a        11878 c         9936 g        19356 t
ORIGIN      
        1 aataggaatc ttactaacct tatgttaact aatttactta gagaatttca tgaacattac
       61 aacaaaaaaa gtacatttct tcatgttttt ttcggatatt tgtgcttttt aaccattcag
      121 cagctgaaaa catataaaaa gatatgtatg tattctttct gaaattaagt tcatttctat
      181 gcgatgaagc atgttttcaa aatattaccc tttcttagat taaacttcag agtattcaaa
      241 taacggaata aggagcctta tcccgcatta ctgaatgaga ctgtgacata agtaaaaagg
      301 tgacttcaaa ttccttcttc ggaatttgaa atcaccttct ttataattat aaagtatcgg
      361 tttcacagca tttctgaatg attcctagaa tcttaaacga atgattggag ttatcaacat
      421 tttttcactc gtttttagcg aaacaaaaaa tgacatttgt cacagcctca ttaaattatt
      481 gattcttttt ctttataatt gaattgtcca tatttaaagt gtctagtact ctacatgaat
      541 aattaattgc tttcactcaa atacttataa tctattaact tgtagttgtt taacttttct
      601 gaaaaagtta gacccacgta ataatcttcc cagctttctc tttcaattaa ctgatccaat
      661 ttttgataat ccataaattc atcatgtatc aaaaagaata aataacttga tattttagca
      721 tctagcttaa tcagattaaa taatgtttgt ttaaaattgc taccattaat atgactgata
      781 gattctctag cttcttcaca taaacttttt actttattaa ttactacttt gtcatattca
      841 tttaacacac tattattacc attacttaga cgctcagatt ctacgaaaca actcaaatca
      901 gaaatttccc aaaaaaattt tttgttaaaa tccaatttcc tcaactcctc caaactacgt
      961 aaatgatata gtctcataat aaaataaaac ctctcagcta atgttgttga aattgtccac
     1021 taaacctaat tgatattgct taggagatac acctgcgtac tttttgaata ctctcgaaaa
     1081 gtgtgaagaa tctgaaaatc ctaaacctaa agaaacttca ctgatagtat ggtttgattt
     1141 caacattatt ttagccgtag aaatttttcg cttagagata taattataaa tcgtaatatg
     1201 catttcattc ttaaagcttt ctcttaattt agtttcactt acagaaaaat aattagctat
     1261 ttcctttaca gtaattcttt tatagatatt taagccgatg aattgaacga cagaagaaat
     1321 taagggagaa agatgctcta tcttagtttt tccgatttcc ttagtataat aaacaatccc
     1381 agaatccctc acttttaaga cctcaggtaa cgatacagct aattcgtttt cttgtatcaa
     1441 agcatctcta ctttgatacg cttctattat atccattcct aatgttatag acgtttgtgc
     1501 taacttttca aaaacaatga tagaataatt tttttcagac cgaatcgtat ctccactaac
     1561 acttggaatt attccattac tcagattaaa aaccatgttt tctaaatttc gaatatctcc
     1621 atttttaaca tattccagta ttttattttc ataaagaaac agatatattt ctggatcgta
     1681 attttgtgat aacagattat ctattttgat ttgctcgata gttttactta gattttctgt
     1741 atattcaatt aacggttcat ggaatagatc ttctattttt ccagtaaaaa aataatgaac
     1801 taaaatcaga atatctcgaa tgtctcctaa agaatacaaa gtcaaatttt gcaatgaatt
     1861 atagagtcgt tctctatcag ccattgcaac attcaaattg ttcattttta gtttaaaaaa
     1921 aactttatca atattatttg ctctaaatgg accaaataag taatatctgg ttttaaacat
     1981 gtatagaaga aacatctcat taaaatcacc agtaaagaaa tggaaatctt tagttttttt
     2041 aatattatca gttagtaact ttttataatc ataaaaaaaa taagtagttc tatctgagcg
     2101 atactctttt ataatactga attcggaatt aaaaaccacg ataggtaact ttaaacaaac
     2161 atgaagagac tttaataaga cttctttatc tgaaaacact tttgtttctc tctttccttt
     2221 ctacatcatt aaatagtatt ttatacgtta tctaaaatac tattcctctg tcaaaataag
     2281 ctaacttact tcattataaa atatccactt atcgctttca tgtatattcc accgtaaaaa
     2341 gtacatttta tcaccgagaa ctcatcattt ttcatcatga tgaatactat ttacatatat
     2401 ctagttcaaa gacaataatt attatgatcc gttattatct aagcactata cagataatag
     2461 gaatttttcg tttctaaata ttccaatttg ggaatccaat aaaaattttc gttcaaatac
     2521 gtgtctattc gaactctttt ctagttgtta attatgaagg gggaagaaat aaaaaataca
     2581 atctatacac gaataagaat attatccaaa aggaggtttt agaaaagatt tattatacgt
     2641 aaaaagctca agacattttc ttagccataa ggacatgttt gatatttaag aagatatatg
     2701 aattttcttt tctaagtata aagataacta tccattatca actaaaaaaa catttttgaa
     2761 aataaacatc agcagtccta tcaatgcatg tgagcttata gaagttattc gcagcgcagc
     2821 ataattgcat tagcttcttt tcgtgttttt tgcctctttt ccctttaaat cttaatggag
     2881 gaggtgtgca atatgtcaga taacgacgaa cgcgtaaatg cccagttgat tcgattcttt
     2941 gaaaaggtca ttttgaacac cgctaatact tattataaaa aacaagaaga aattcaaaaa
     3001 catgaacaac ttgatcaact cccaccatat ttgtttacta aagatactat aaaatttaaa
     3061 caaccgatta atgttttaaa tgtgactctc cttgttgaag atataaaatt agcaaaaatc
     3121 cttcctttgt tgaaagaacc tgaacaaatt tttcttatcg aaaaatttat ctttgataaa
     3181 actgataaag aaattggaaa ggttctgggg attacaagac agggtgcgac aaatttaaaa
     3241 catcgacttt acaaaaaaat tcaaaaaatg ctggtttaat atctgcataa taactaattt
     3301 tgtatgcttc tttgctcgtt ctcctccttc ccaaagaaat agattataag tccatgggaa
     3361 gaaggtagat ttatgaattc tttaaaagaa gatttcattt tagcaaaggc tggaaatgaa
     3421 gaagctgttg aggcaattct caaaagattt agctctttga ttcataagca atcctggaga
     3481 actggaaaat atgatcaaga ttgttatcaa gaatgtatgc ttgcaatata tctagctatc
     3541 tcgaaattcg aaattaaaga ataactcaac taagggagtc tgtgacataa gtaaaaagat
     3601 agtttcaaat ttcgaacaat aaatttgaaa ctatcttctt tataattata aagtatcgat
     3661 tttacagcgt tctataatga ttcatagaat ctaaaacgaa tgattttaat tatcaacgtt
     3721 ttttaattcg tttttagctt aaaaataact tttgtcacag tctcactcct aaaattactt
     3781 tatttacgta tgaagtgggc aggaggtcga atccagaact gggctatggt acttcaacga
     3841 ggacttctcc tctagaattg aaaagcatgc gatttacctt ccgtaacgac ggtagattta
     3901 ttcaactatg tgtccaatca aaatttgtct gatttcaaaa atagtcaaga gaattttggt
     3961 tgttaaaaac tcttatctat caacttccac agtttagttg acaaaccctg atactaagtt
     4021 gggctatgat acacttttct tgactatcag ttacgttacc ggaaggaatg aactctattg
     4081 agaaaaacat atgatcgtaa atttaaacta aagatttcac aggatatcct tgagaaaaaa
     4141 gtaactacca agaaaattgc tgaagaatac aatatctctc gtccaacaat ttcccgatgg
     4201 gtttcagaat atcgtcgata cgggaaaaat gcttttgctg gacaaggaaa aagattacct
     4261 gataaagcag atttctacat tcttgaacag gaaaataaac gactaaaaga ggaagacgag
     4321 tgttctacga acacaaagat cgatacggat ctccagggat cactcaaaaa ctttacgatg
     4381 aaggaatcga aatcaataaa cgtgttgtag ctgttcttat gcgtgaaatg aatttatgca
     4441 caagaggatt tcatcgacgc aaatcttctc atggtagaga aaaaacaatc aaagaaatgg
     4501 taaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt gggtgatgga
     4561 tattacttac attccatgta atgacggtcg cctctatttg tccacctata ttgacttagc
     4621 aactcgtatt ctacgttact atatggtaga ttcacacatg aaaaaagaga ttgtcgtccg
     4681 tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata gaggtagcca
     4741 gcatcggtct tttatttatc aagagctgtt gaacgaccac cacatcacac attcagtgag
     4801 ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat caatcaaatg
     4861 agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt taatccaaga
     4921 ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga tgactccttg
     4981 acaatttgaa gcgaagaaaa taagggatct ataatatcta gtgttggtga cgccttctgt
     5041 tgccaatact ttttcttttt tctaataaac gcaaacagac accttaaaat cctttagaat
     5101 aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt cctattcaga gtttaccaaa
     5161 gagttactag atattctaga cctaaatctt atctttcatg aagatgcctt tcgcaaagag
     5221 cgaatcaatg acgaaacatg ttttgttttt gatggcacat tgacctatca accggaggaa
     5281 tgctttcatc gccactatca aaatgaacaa acgatcatta aatagggctg gaaaaaagta
     5341 tccatcttat tgaatgatgt cagtaactac aagaccattc tgcggattaa caaacagcgc
     5401 ttcaaatgta aacactgtgg aaagactttt ttagcagaag attcagttag tgatcgtcgc
     5461 tgctccattg ctcgcagagt caaacaagcc attcttgagc tattaagcga acctctttct
     5521 atgtctttga tcgcacgtat gaagcacatt tctccgacca ctgtcattcg aatacttcgc
     5581 agccttcggc ctaaaaccat ttctttgagc cagctgttac cagaagtggt gtgtttcgat
     5641 gaattcaaat cagtgaaaaa tgtctcggga gccatgagct ttgtcatgat ggacggcaag
     5701 acccatcaac tgattgatat tgtggaaaat cggcaactca atcctttacg cgactatttc
     5761 ttgcgctacc ctataaaagt ccgtgaacaa gtgcgtctag tggtttcaga tttctataca
     5821 ccctatcgca cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc cgatcgcttt
     5881 catattagcc aacacattgg tcgggctttt accaaccacc gaattcaagt aatgaagacc
     5941 tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat actggaaact acttcaaaag
     6001 aatgcgtggg aattgaaagg tcagcatcgc tactggcgtc cgtcatttcg agatcatctg
     6061 acagaagcag agatagtcga tcggctatta tcttatgatg attccttaaa gcgaggctac
     6121 gaagtctacc aggattttct ttccgttatc agaagacaag atgttcctga attcgtcgcg
     6181 ctcctaaaag aagactacaa ggagttgcca gagcactacc aaccagtgtt cacgaccttc
     6241 aagaaatacc aaacagaaat caaacgagcg ttgcgcgttc cctattccaa cggccctata
     6301 gaatgtttga ataaccatat caaagtacta aaacgaattg cctatggcta tagaaacttt
     6361 caaaactacc gagaacgaat ctttttatat cgaggaaaat attttaagaa aactaagaat
     6421 actacccaac tgactaaagc cagaacaaca acacggctag acaagatagc tgtctagccg
     6481 tgtgagtatc tcgattattc tattgaattt cttcaccaac actcattgac gaagagccaa
     6541 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
     6601 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
     6661 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
     6721 gtaaatgtag taatctgtgc tttctttttt ctcaatacta ataactaact tatggggtaa
     6781 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
     6841 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
     6901 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
     6961 ttccttgcca tcaattcgaa cgatggcata tttgatagca tcttcagttg tttcattctc
     7021 cttttctata gagaatacaa ataagggaat aaaagaggta ataatcaaaa tgccaataat
     7081 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
     7141 aaacatatta gactttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
     7201 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
     7261 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
     7321 gggatttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
     7381 taatgattca atacttttat agctacccga tgtttcaaag atcgtcgaaa aatcactatg
     7441 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
     7501 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     7561 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     7621 gcccactgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     7681 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     7741 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     7801 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     7861 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     7921 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     7981 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     8041 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     8101 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     8161 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     8221 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     8281 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt tcatagggaa
     8341 cctctttcta taccttgaat ttacacttta agtgcaacac ataaaaatga gtcataatcg
     8401 atatccaact agtataagtt tgtcgagaac actcctagaa ggaaaataat tatgagccag
     8461 tatcgtcatc ttactatact cgaacgagaa agaatctttc tgcttcacga agaaggattt
     8521 tctattagta taattggcaa cgacattcag catagcccgt ttactatttt acatgaactg
     8581 agaagaaaca agaaaaaaac tattcacctt ccaaagcgga aaaaaattat gcttatcgaa
     8641 gaaaaaaatg cggacgttat cacattctaa atgatccgtg agtttggtca attgttcgtc
     8701 atttatttgt cgattttcag tggtcgccag aggaaatctc gaatcgtttg aagcatgaac
     8761 atatggactt ttcaattagt tttatcacga tctaacgtgc aatttgcaat gatcttttcg
     8821 atacagaacc cctctcacac ggcaataaag gcctgatccg ttcattgcac catcgaggaa
     8881 aaacaagaca tacaaaaaat catgtcgagt ctcgtggaaa gatttgcctt actcattcga
     8941 tccatggccg tccaaaatcc gcagatgata gaagtgattt tggtcattgg aaagccgata
     9001 ttgtagctgg caaaactggt ggtacttgtt tagtgacttt gacagacaga aaatcatgct
     9061 tccttttagc aggaaagaag gtccccagaa agaaagccgc ttatgtgagc gagaaaatga
     9121 ttgaactttt aagcagtatt ccaaatgagg cggtcaaaac gatcactcca gatagaggaa
     9181 aagaattctc caatcatttc gcagttacag aagcattgaa taatatgccc ttttattttc
     9241 cagatcctca tgcccttggc aacaatgaac aaattaaaat acgaatagac tcattcgaga
     9301 atacttgcca aaatcgaaag agatagctac ggtagataac tcaacgataa atcagatgat
     9361 tgaaaaactg aataacagac ctagaaagtg tttagattgg agaacacctt atgaagtctt
     9421 ctttgataaa gtgttgcgct tgatttgaca attcaagaca taataaaaag cagatcaaat
     9481 ccaaataagg gaaaaatgat ctactctggt attatcaatt aattggccaa gctattatgc
     9541 cgttttgcta aacgtctact tcttccagta ttgaccttga taaaacaaca atcaggatcc
     9601 cgttttaata ctctatgaac tgtgtcaata ctaacatgta aagttgcagc aatatcagct
     9661 aatgtactcc catttaatct catttgcttt gcaaatcgat aacgccgata agcatcaata
     9721 ttcttggaat ccatagacaa taatactttt cttaccatgt atttcctcca aaaattgaat
     9781 tttaatgagc catcatttct tgagcaattt catgtttaga caatgaagct ggatagaaag
     9841 taggccaatt atgcaattca ttatagagtt tttcttgact ttctccacca aagaaaatat
     9901 ggaaatgctc tgccttactt ggtgcaattc catgatcact aaattgaata tatttaaaac
     9961 gaccagcacc tgcatcatca gtttcaaaat taaaacgaac accacggttt cctttttcat
    10021 aattcaaaat tttgtatcca acatatttgt atgtgtattg atgatgttca ccgttgacta
    10081 caaaatcaat ggtgttgtct ttaatgttga tagtatcaat atctgtctta tagccagtag
    10141 tataataatc tttgtactcg gcagctgtca tgtctttatt gatttttgct ttgtaatcga
    10201 aaacttcatc caaaattcca tcttttaaca acggataaac agattgccat tcgcctgtat
    10261 aatcactcaa ttcgcgatcc ttcacatcgg catctttaaa gtacccatta gcaactgtct
    10321 tttcatcctt cgcttctttt tctggaacta cctcttcacc agcaaccata gttgtttttt
    10381 ctaacgcttt tagattgtct tccattactg aaacataatc ttcaccatta tccatttgtt
    10441 cttttgtaag actttcaaga ggattcagta cttccaattt tatcccagct tcgttggcca
    10501 aagtttttgc aattttatca ttagcattct tttcaaagta aatataattg atcttatttt
    10561 ttttaacata ctcttttaat tcagctaatc gaccagaaga tggctcttct tcaggactta
    10621 gtccagcaat aggaacttgt ttcaatccat aatccaaagc taaatatcca aaggctgcgt
    10681 gttgcgtcac aaaacttttt tgtttcgctt ctttaagact agtggtatag tcagcatcca
    10741 accgtttcag tttagtcaaa tacttttcag catttgtctc aaatactttt gcttttttcg
    10801 gatacaattt aactaactga tccttgatat ttgaaacttc cttaattgcc attttgggtg
    10861 aaacccacgt atgaggatct agttcatgat ggtgatcttc ttctccgtga tcatgatctt
    10921 cttcttcact acctggcaat aaaatcatgt cttttgttcc ttctactaca ttaggcccag
    10981 ctttcttcca actttcttta gcttttggta cccatgattc catgttttcg ttatgatata
    11041 caaaaacatc agcatcatga gcttttgcca tatccttagc tgagggctca tagtcatgag
    11101 gttctgatcc cgctggaacc attaaatcca cgttcgcttc atcgccgacg atgtttttgg
    11161 tgaaatcata cattggatag aatgttgtta caatttctaa ttttccatct ttatcattcg
    11221 atacttttgc atcattagtg ctggagcaag ctcccaataa tcctagagct gctatcccaa
    11281 taagtcctac actgattttc ttcattcttt ttccttcctt aattatgcta atttcttcat
    11341 tttatttcgt ttatgttctt tgataatttt taatgccctt tttctagttt taatattcgg
    11401 gcttttaagt cgtcgatatg cactagatat atccgttttc tccattagaa attaccccca
    11461 atcactactt atcattattt gctttccgcc agtaatttat caaaactctg tttgatcctt
    11521 tcttcatcca acatttttcc gattagaata atagtggttt ggtctttttg tcgagtggtg
    11581 tattgaaaat taacctgttc gtttacctct tgcatggcaa ctataaaatc ctgctcttgt
    11641 aaagcaatca atcctttaaa acgatacagg ctcccttggt tggttaaaat cagccagtca
    11701 atccattgga gtaaaaaatc tttttttatg tcttgatctg aagttaagag aattgattta
    11761 aactcatgta cttgatggtg ttgatgatag tatttgccat tatagtgatt ttctgatgaa
    11821 tcactttcaa aaactaaggg atgattaaat tttttcaaat tataaaaatc ctttttttga
    11881 atcgtttcac tatgtgtgaa cattcgaata tcggataaag ggttaatact tttgatttct
    11941 ctctgtaatt tttttagtgc agataaattc aagttttgtt ttctagacac aaaaatgcga
    12001 tctgccatga caagttgctt aagggcttgt ggttctcttt caataagttc aaaactgtca
    12061 ctatcaataa cagtcaataa actatcgata tatactcttc ctcttagttg aggcgctgtt
    12121 aaaagtgttt gaatgattgg ttggggatct gctatacctg tagtttcaat gatgatttgt
    12181 gaaattggtt gttgatgttc ttctattgtt tccagtaaag aaatcaatat tgctaataaa
    12241 tcactgcgaa gactgcaaca catacatcca ttatttagtt gtaacacccg ttcctcgcta
    12301 tgaactaata atttgtgatc aatcccaact tcaccaaact cattttcaat tataataatt
    12361 tcttccctgg ggtagggtga gacttgcaag gcttgattga ttagcgttgt ttttccagcc
    12421 cctaaaaacc cagaaacgat tgtaactggt atgcccattt tgtcaccttt ttaccactta
    12481 gctatttcac ttctttaaaa attgcccgtc tgcgcaattt tggcgaatac ttttttaatt
    12541 ctaaccgttc agggttattc cgtttatttt tgctggtgag atacagacgt tctccagttt
    12601 cgacacattc caaaataata ttttgacgca taaatcttcc tccttcaata cttatcgatc
    12661 ttcaagatat gcttgaattt gttgtgcatc ttcaaaagtt aactcgccag attccaattg
    12721 tgaaactaaa ttttctttca tatcaacagg tatctcccaa taaccatcga tacattgaaa
    12781 taactcctcg tcatactctt caaaaattgg atagattctt aaaaaatcat caaatgataa
    12841 gccttggtcc tcatagtaat ctttgactaa tccattttca attgcctgtt gggtcggtaa
    12901 acccgtttca gtaataattc ctctctctac catcgaacga taaaaagtgc atttatctag
    12961 cgattgctgg gtcgtttcaa gataatactt taatccttct tcaactaaag gtgggtaaat
    13021 aatttccatt ttgattccct cctaaaagtt atatattaaa ctattttgaa gctcctttaa
    13081 ttactttctt tccattatag taccctttta aagatacatg atggctacgt ctataatcac
    13141 cattcgcttc gtcaaatgaa atttctggtt ttactacagt ttgatgagtt cttcttaacc
    13201 gtttcttcgc cttagatgtt tttcttgctg gtactgccat gatatttcct ccttttacca
    13261 acttgctttt tttactccag gaatcaaccc ttgatgggca agttctctaa acttaatacg
    13321 ggacataccg aactttcgca tataccctct tggacgacca tccgtctgat cacgaagcct
    13381 cagacgattc ggatttgaat ctcttggcaa tttagcgaga gcggtgcgat ctcctgcggc
    13441 ttttaattcc tgacgtttat ctgcatactt ttcaatcatc gccatttgtt tctttgcttt
    13501 agcaattttc gacttttttg ccatatacct ttatactcct tttattttga tttagacacc
    13561 tcaaatcgta atcgttacgt tttgacactg atactttacc aacaagagtt gctcttgtca
    13621 acaataaata acatgaaata aaactcatat gaatagataa ctatttgaaa acgaatttat
    13681 cggtacatag agataatcaa atcatttaaa agaatatgca gtttaggctc tgaaaaataa
    13741 gaattcactt ttattagtcc aatcaaacac aaataccgtt ttaagtagct tttcctctac
    13801 ttaaaacggc aatatgaaag aaactataac tttttattca atcttttatg tttaactggt
    13861 ttatcgccaa ttttttaaaa ctttgattat tccttatatt ataagaatag acattaagtg
    13921 aactaattca atcatactga ttctttcgtg tattcaaaat tagatgctgt ttaataaaat
    13981 tattcttttt gaaaaagaat tataactata tcccaaaaat tgtaggttaa tttaaagcct
    14041 atttcatcat cgtgattgag cttctttttc atagacgaat ttctcagata gtttagtttt
    14101 ctttatgaga tttttgtata tttctgacct tcgatacaac tcattatgag taccttgatc
    14161 aactagtttt ccttgatcca tcactacaat tttatttacc ttttcaatag acttcaagcg
    14221 atgcgatata attagtactg tcttatcttg tattaaacga tttaaacttt cctgaatacg
    14281 tttttcattt tccgcatcta aactggcagt aatttcatct aaaattaaaa ttggcgcatt
    14341 tttcaaaaaa gcccgtgcta tggataggcg ttgtcgctct cctccagaaa gtgaacttcc
    14401 gttttctccg accaatgtat gataaccgtc aggtagtttt tcaataaagt cttcgcagtt
    14461 agctaaacga gctgcttttt tcacttcctc atctgacgct tgactatttc caatacgtat
    14521 attttccaaa atacttgtat taaacaaagt tacctcttga aaaacaatag cgattttgct
    14581 aaacaaagaa gctggagata cacgtttaat atcataccca tcaattaaaa tacagccttc
    14641 atcataatca tacagtctag acactaattt caagatacta gttttcccgg aaccactagc
    14701 acctactaac gcagtcactt ctccttgttt agccgtaaaa gagatatgat ctaaaattgg
    14761 ggtattgtta tcatatgaga aagagacatc tcgcaactca atgtcaaaag atttcagagg
    14821 actatctgat ccctcttgga tactggtttc cttcatagct ctaattcgct gtattttagg
    14881 agctaaataa aatatttcaa gcactgcttc tttcatagaa tcgaaggaat cctttatttt
    14941 tattgcggct agtaagtaac caacgacgta gagtatagtc acttcacccg ttaacagtaa
    15001 gtgaacacct actagaatga ctactgctaa actaacatat gagaaaatag aggataaggc
    15061 catgactgaa aaagtactta attctacctt taaatgaatc ctttcactct cttccatttt
    15121 tttgaaaagt ctgtcttgaa cttttttaga tagattaaag ctattaatct cttgctgcaa
    15181 ttctatagtt tcttgaaatt cttccgagtt ttctctcaaa gtatcgtaat atttagtatt
    15241 ggctttcgtt tgggatttct ttgataacaa gattaagaca aaactaaata acgttggcaa
    15301 aataacagct aatcccattt tgacattacc aaccaaaagc atcactgaaa taaaagggaa
    15361 aaacagagcc ataccgccgg ctttaggtat tgcatgactc atcgcatgct caataccttc
    15421 aacgtcagac atgatagttt gagataaatc tgataaatta tgttttgaaa aataggataa
    15481 tggtaaattt gacaaatcgt ctgcaatttg cactcttaaa tgcgcacttt ctttataagt
    15541 actattatat aagttttcat actccctatc taacaaccaa tacatgaaaa gcaaagtaac
    15601 aaacgatacg gcaaaataaa gccaacgggg ctttgtattt tctaaaacta attcgtctat
    15661 cagcatcatt ataatgaatg ctggtaccat attaatgcag tatacaaaaa aactagaaat
    15721 agaagcttta gttaaagctt ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa
    15781 acttttcatt gtcaagcctc caatcatttg cattctcata attagtgacc aattgtttat
    15841 acacttgatg tttagccatt aattcattat gatttccacg ttctactatt ttcccttctg
    15901 acaagacaag aatttcatct acagatttaa tgctggataa tcgatgagct atcataataa
    15961 ctgttttttc ttttatcaga tgttgaaaag ccttttgtag ttcatactca ttatctggat
    16021 caatagatgc actggcttca tccataataa caatctttgc atcttttaaa attgctctgg
    16081 caatagctat acgttgtttt tctcctcctg acagataaac tcctttacta ccaattaagg
    16141 tatgctctct ttcgggaaat ttttctaaaa tcgattcgca accggctaat cttagcgcgt
    16201 caaatacttt acttgctggc gcttgaggat ttgctataga aacgttatcg taaatactct
    16261 gattgaatag tttcgtatct tgaaagacaa aagcaattgt gttaataatt gtgtcttttg
    16321 aatagtgatt taaaggctta ccaccaattt taatctgtcc cttatccaca ttgtagaaac
    16381 ctgaaattag tttagctatc gtactctttc cgcttcctga ttttcctact aatgcataaa
    16441 ttcttttttg ttctaaaact aagttgaaat tttctaaaac acactcttct ttgtatgaaa
    16501 agctgacatt ttcaaatgca atgtcacaat ttttaatctg tgtctcgttc ccatatttta
    16561 gttggtcttg cttcatttct aaataaaggg agtctagtgt ttcgactgca tatcttgctt
    16621 caaacaagtg catacttaca tacattattc tcatgaaaga aacaaaaata attccactta
    16681 aaaaaaacaa catcaacaag tcaacagcta atatcttggg agtggtaaga gaagttataa
    16741 aaaaggttat aggaactatc aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa
    16801 caaaaggtgt tttcccactc aaagaatact tatatgcaaa cttagcataa tcgttaattg
    16861 ctttattcaa ggctttaaat gatgttactt tagcaccaaa tatctttata acttgaattc
    16921 cgcggatata ttctaccgtt tcagagctta acacatctaa cgattcttga tatttttgaa
    16981 taaaatttgt attctctgtc attttcttta acaagaatac accaattact aacatggcga
    17041 ttagaaccaa tccaatttta aagttaatga aaaagcctaa ggcaactgat aaaacaggtg
    17101 ttactatggc ttgagcacta tctggaatca agtgagcaat tgccatatgt gttttagccg
    17161 cgttgtcatc aattgtcttt cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac
    17221 ttgcttctgt taaaccgcta attccctttt ttctcaaatt agtctctaag cgaaacccaa
    17281 ggatatgaga aaataagcca gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa
    17341 cagtttgaac cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa
    17401 gaaatttgta aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata
    17461 aagtggctaa ccatccgtag tatttcattt tagggacata actaaataat tttttgtata
    17521 ctttcatttt ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg
    17581 ctttccgtaa tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat
    17641 tttttgtatt tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct
    17701 acaactaaca ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt
    17761 gttgcagcag attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat
    17821 ataggaatcc actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga
    17881 ggaattgctc gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta
    17941 atcgaactga taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa
    18001 acaattgtgt aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat
    18061 atgattccag ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca
    18121 atactactaa ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca
    18181 ataaaaatat aatcttttgt tttcatatat attctcctca attctatttc gttatgtcat
    18241 taaaaatctg ttctatcaaa gttgaatctt ctactctaag ttttatatct ttttttattc
    18301 caccattttt taaataaata attctattgc atgttttata aagaaattcc ggatcatgag
    18361 tagctataac tactgcaccc ttttctttta agtctttaat aaggtcagca atcctttcca
    18421 tattaatata atccaaccca cttgtaggtt catcaaatag aaataaatta gtttttgaca
    18481 atgaagctat agaaaccaat aatctttgtt tttctccacc ggataaatca aacggtaatt
    18541 gatctttcat tttataaagt tccatttttt ttaaaatttt ttcttgctga tctttactaa
    18601 ctatttcatt ccccagggct aactcgctcc tcactgattc tgtgaaaaat tggtaatcca
    18661 tttcttgcat tactaaaaac ggcgatgaca aatttttttt attttcgatt atctttcctt
    18721 tggtagggcg aataatcttc atgagtattc ttagtaaggt tgttttcccc acaccgtttt
    18781 tccctaccaa accaataaca tctccaactt tgacatcaaa atttatgtct tttagaatat
    18841 tttcataagt aatttcttca acttttaata catctgcact taccttttcg atatagggaa
    18901 cttcaatcga actattaaaa atgtcaaatt tccttaatgc gttattgttt tcagttttta
    18961 acaactgaat agttgtagtt tttacagttc tatttttaac aaatagaact tgatcaatta
    19021 attctttcaa atagtaaaaa cgatgttcag ctataacaat agtaaatcct ttatttttta
    19081 aatcacaaag taagtcagca agtttcttag tcgctttgaa gtccaaattt gaagacggtt
    19141 catccaatat aattaattta gagtttagcg ataaactaca tccaatagaa attttctgcc
    19201 gttcaccact tgatagcgaa aaaatgtttt tatctaatat atttgaaaga tctaataatt
    19261 cagctaattc attgattctt ttatccatat gctcttttgt aaatccaaag ttttccatcg
    19321 aaaatactaa ctcactcgtt gtatttgttg taaagaattg tcctctagga tcttgactta
    19381 cgtagccaat attttttgat atactttctt gtgttaagga ttgtagcgag ttattttcaa
    19441 ataaaatttc cccaaccaat tttccgtcat aaagtcttgg aataagtcca ttcattaact
    19501 ttaatagtgt ggatttccca ctcccagaag aaccagtaat caatgtaaga ctgccttttt
    19561 ttattgaaaa attaaggtca acaagcgctt gttccttaac atccttatat gaaaaagaaa
    19621 cattgttaaa ttcaaacaat tctttcacct caaatcaaaa tacttaagaa aaaaatagta
    19681 acggaaaaca ggatgatata gtctaataag ctcatgtttg ttttatagta agaacttctt
    19741 ttcacaggag attctatccc ctttgttatt gcattagcgg tcatttcttc agcaatcata
    19801 gttgcacgta taagaacggg aactaacaga aattccaaat attgcagagg ctgaaaaaaa
    19861 gacaagcgaa tgtttctcat ttttaaagat tctttaatca ttcttatttc agaacgataa
    19921 gtcggaaaga atctgatcac tactgtaata cttaacatta tttttttcgg taaatgtatt
    19981 ttttctaaag aggataataa ttcattgcta gtatagacac taaccaacaa ccatgagatc
    20041 attacgaccg gcaacattcg aaatacaata tataaggatg tcgcaaaaaa tatagtaaaa
    20101 aactctatct tgatagcatc aataattttc attactaaaa acagaactga ataaatcaag
    20161 atgaaataaa tacatgtctt tattttacga aacatcaata ctaaaactgt agcaataatg
    20221 aaacacccca cttgaaatat atttcccttt cctaaaaaaa taatgaccgg aaaaaaaata
    20281 tttaaagtaa tcaaggttaa cggattgatt tttgacactc tttcacctcc aaaaaataag
    20341 atcgcactgt tatcttaagc tgataaaaat gcaccgttat tcagataaca gtgcgatctt
    20401 atcaaaaagc ataactgaga acaattctca atgcaagctt tttttaatta actcagtaat
    20461 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    20521 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    20581 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    20641 tctagacaac agactgtctt tttcgtcaca aatgatgtaa ttttttcaac aatatcttga
    20701 tatttggttc cagctgattg gaataaaatc aaatcgaata tacgtttatt ctcaaaaata
    20761 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    20821 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    20881 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    20941 gcacccgtag ttacgcctgc ggtcaggcac agttgcctta tcttagtttg ctgaaatcct
    21001 tctttttcaa aatggcttag tccactttct attaactttt ggtgagttat aatataatct
    21061 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctttattatc ttacacagga
    21121 cttgaagttt caatcgttaa cgtttattac tgaactcatt ccttagagct aaactaatga
    21181 gaaagagcag aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    21241 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    21301 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc
    21361 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    21421 aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct
    21481 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    21541 aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct
    21601 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    21661 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    21721 agataggaat attggtatct tttgatactg ttttcattgg tctatcatcc acactgcttt
    21781 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    21841 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    21901 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    21961 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    22021 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    22081 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgagga tcttccttcc
    22141 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    22201 ctttgttttc ctctttgttc gcatttttaa ctaattttgt aaaccaagca tttcctccag
    22261 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    22321 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    22381 ttttatcttt tgctatattt tcggtaatat ctgctaaaat cgaattagta actactactt
    22441 ttaatttttc gttcgaagcc actttatctt tgtctttact atttattgaa catgccccga
    22501 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    22561 taacgatctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    22621 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    22681 aataaacaat ccaagcaatg atgaacttgc tccgactgtt gcagaaataa atatcatagt
    22741 tttcaagcta tttgcatata aatatgctgt agctgctggt gtaattaaca ttgcaacaat
    22801 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaaccatcaa
    22861 tagataatga taaaaactga cattcatccc tattgctttt gccattaatg gatcaaaaga
    22921 agtgattaat aattctttaa aaaatagaac tactataacc aacactaaaa ccgaaacgcc
    22981 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata aaatatgaaa
    23041 taaatccgtt gagctatttg caactcctat aaggataact cctagtgcta aaaaagaact
    23101 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    23161 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    23221 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    23281 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    23341 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    23401 attattcccc ctctccaata aatatagtat ctccataggc tttttttaaa ttttcttcat
    23461 taaatacaga ttctacactt ccgtgagcaa ttagatttcg attcaaaaga ataatattat
    23521 caaaatactt ctttaccttg cttaaatcat gatgcacaat tagcactgtt tttccctgat
    23581 gtttcaattt ttttaacgta tccataatta ttcgttcact aactaagtct atccctacaa
    23641 atggttcatc taaaaatatg aaatccgcat tttgagcaag acatcttgct aaaagtacac
    23701 gttgaaattg tcctccagat aattctccta tttgatgatt agaatatttt cccatatcga
    23761 ccttttccaa agcctttgaa actctttgcc attctgcatt tttaattctt tgaaaaactt
    23821 tcattccggc atatgttccc atagatacac attcttttac ttttattgga aaagtaaaat
    23881 caatatcagc tttttgctct acatatgcaa tcttcaccag ttttttattg atatcttcat
    23941 tattaatgga gacctctccc tgatgaggga taatatttaa cattgcttta agcaatgtgg
    24001 attttccagc accatttgga ccaataatac ctgttaaagc accttcttgt atttttaaag
    24061 aaatctcgtt caatgctaaa aaatcgttat aacaaacatt aagctttttc acttctaaca
    24121 taactttctc cttctaaatg ataatgatta tcacgatcat atggtataca aaaattatat
    24181 tttagtcaac cctaaatttt atagtttaga tttttttctg ttgctgaaga ttagaatgac
    24241 aaaactattt gaaactacgc gtatagtaag tagctttcaa aatatataaa aaaggagcgt
    24301 tctcaaatga gaacatgctc ctaaaactac tttattggcg tatggaaaaa ttatacttga
    24361 agtggatagc gggttgaata agttgataag catcaaaaca tttataaaaa ttattcaaga
    24421 ctatgtctgt tccatataca aatatgaaaa tcagtaaact ccatttaact tttttcgtag
    24481 tgtgtctttt tatacatgtt tatcgttatt aatagtgaat aggaaaaaaa ctatttttga
    24541 tcattgacaa ttaaatacaa tttttgaata acgatccctg ctattcgagc tagaaactca
    24601 agtacgccat gacctgatta gcgacaaata cttagagatt tctttttgtt ctgggaaaac
    24661 aaaatgccat gacaatagca gcaaataatg atacttccgt aaggctcgcc gtaaactggg
    24721 gagaggtgaa attcctatgt gcttacccaa agcaattcaa aaatttcata actactattt
    24781 taggagatga ttttatagaa tccagagtct acctgaataa aaaacaaacc tgtgcatttc
    24841 taggaatcga taataatact ctagataagt ggattcgtat atataatttc ccagtaataa
    24901 gagttggtgg tgtagttcgc tttgacgtag aagatattaa agactttatg aatacattta
    24961 aaataacttg aaattttctt ctcgccgcat atactaaaat tagtgaaata tactgcctta
    25021 ctttttttaa ggagtgagga ttttgcctac aaagctttca aatgggaaat ataaaactaa
    25081 tctgagatat ccaaaaaggt ttaaagaaat aacaggaatt gcatcagaaa agtatcaaaa
    25141 aacatttcca aataggcaat tggcgattaa agccgaaaat gatatgaaga agaaaataga
    25201 aaaagttttg agagaggaga atgcaaactc attagaatta aaaggaaaaa taacgtttaa
    25261 aaaattttac gaatctaaat ggcttcctag atatgaatta ggtcaaacaa ttagatcaaa
    25321 caggcctcct agtgatataa ccattagtaa tacaaaagat atatttcggt tgcatatttt
    25381 acctatgttt ggtgaatacg caatgaatta tctaaatatc aatactgaga taatatcaga
    25441 tgaattaacc aaaaaatcga aagaatatgc aaatataaaa atcataaaag gatatgttcg
    25501 ttccatgttc gatattgcag aaatactaaa ttatatcgaa tttaatcgaa ctactaaaat
    25561 tattcaaagt atcactgcac ctaaaaaaaa tgctttagaa gaaaaaagaa tacaagaagg
    25621 taagcaagca ctatcatcaa aagaacttac taattggata gaagcagtaa atgatgattt
    25681 taataatcat ttacttactt ttcatgatta cacacttttt atgattaccc tgtatcttgg
    25741 tgatagaaaa agtgagactt acgctttgca atggaaatat attgattttg aaaaacaaac
    25801 ggtccgtctt aaacatactt tagataaata tcaaagaaaa aagtttacaa aaggtcgaaa
    25861 agataccgtc attcaagtac cagaagttgt aatgacattg ctaagtgaat ggaaatcggt
    25921 acaagctgat caattgttga aattaaaaat taagcaaacg ttagatcagt atctctttac
    25981 ttatactaaa ccatctggag aagtaaattg tcctgttcat gccgattacc taaactatcg
    26041 aatcaattca attaaaagaa ggcatcctga cttagttcat ttatctccac acaaactccg
    26101 tcatacttat gcaactatag ctagacaagg tggagcagat atgaatcaaa tttcaaatgc
    26161 tttaacacac tcagatattt ctacaacaaa gatatatgtg aacacaccag atatagttga
    26221 taaggctgtt tttgaagctt ttcagagagg attgaataag tgtgattgat atgtgttctt
    26281 aaatgtgttc ttaaaaacaa aagaagcact ttccaaataa ttgaaaagtg ccagaaacgt
    26341 tgatatataa aggatattaa cgttttgaga attgtgaagc tttacgtgct ttcttaagac
    26401 ctggtttttt acgttcgacc atacgtgcgt cacgagtaag taagccagca cgtttaagag
    26461 ctaaacggaa gtcaggatca acttctagca atgcacgagc gataccatga cggatagctc
    26521 ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa tacgtcatat gcgccttttg
    26581 tttcagtaac agcgaaaggt tgattgatta cttcacgcaa gtcagcatgt ggaatgtatt
    26641 cttcaacatc ttttttattt acagtaattt tgccagttcc tggtactaga cgtacgcgag
    26701 caactgcgtt tttacgacgg cctgtgccga tatattgaac ttgtgccaat gaaattccct
    26761 cctattagat taagtttgtg atgtctaaaa cttctggttg ttgtgctgca tgtggatgtt
    26821 ctgctccacc gtatacgttc aattttgtaa attgtttgcg gcctaaagtg tttttaggaa
    26881 gcataccttt tacagaagtt tcgatcaaac gacgagaatt tttagcacgc aattcgccag
    26941 cagtgattga tttcaatcct cctgggtagt tgctgtgacg gtaataaatt ttgtcagtcg
    27001 cttttttacc tgttaatttc actttgtctg cattgatgac gatgacgaaa tcgccagtat
    27061 ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag tacagatgca acaactgttg
    27121 aaagacgtcc caatggaaca tcagttgcgt ctactacata ccatttacgt tctacttcgc
    27181 ctggtttggc catatatgtt gtacgcacgt tattttcctc caatttaagt tctgatgttt
    27241 gacatacact tcgagtttcc ggggctcttt gtggggcaaa caataccatt taacatatta
    27301 cccgctaatc tagtcaatgt caacgttttt ctgcttgatt gaaaaattta tatgaaactt
    27361 tacttctttt ctttctttta tataaaaggg tcgtttcctg tcaaaatgaa aaacaaaaag
    27421 aggctgtgac aaaagtcttg tcacaacccc ttattttgaa taaacggtgt tctcctgcca
    27481 actcctcggt cacaagtcac aatatccata aaacataaga agctgttttt taaaattgtt
    27541 tcttgtgact cggagctgaa cggcaggctt acaacctctt ggtaaacggt gttcaatcag
    27601 ctgaccctcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gacgtttgtt
    27661 tctctaatac tcaggtcaaa acgctgattt cacaacctct ttttcggtca agttcaacaa
    27721 cagtttttac gcgtcgatct ctggcgcttt gcccaaatga gcttggatca tccagatacg
    27781 tttttctgtg gctgttttga attcgataaa gatatcttgt gttggatagt cgccttcttc
    27841 tccagaaact tcgattcctt tttggtaaag atctgctaga taacgatagc ctgcaacaag
    27901 tgtttccaaa cgttctggaa tagtacgatc ccagcggcca atttcatcag ggatttttgt
    27961 gttatctgca aattcacgca atgtagaata aggtgaaccg tctaatgtga tcaagcgttc
    28021 ggatacttca tctaactgag cgttcaaatc atccatgaac tcatccatca ttgggtgcaa
    28081 tgtaaggaaa cctggtcctc tcatgtacca atgtgtttgg tgaacaacaa cagacatttg
    28141 gcttagatct gcaactaatt gatttaatac ttctttggtt tgttggaatt tcatctttct
    28201 tgcctcctaa cattttctta cacttaaaat aatagtagac tttcaatgaa aagtacaaca
    28261 aaatcattta cctgagaacg attttcagtt cgctttttta tccgtaaagc caatcatttt
    28321 tcagaatcag ctaattcttc tcgcagttga gcaaattgct gataagtctg tgctaaatgc
    28381 ttttgtgtcg aaacaaataa tggatagatt ttctgatatt ttttatgatc agtttcttgt
    28441 actccaactt gctctttcaa ttctatcaat tcctgtgctt cactgatact ttggatcttt
    28501 ccgatgcttc tccagcctaa aatcacagct cctaatgcgg atgcttctgt gacagtaggg
    28561 aaaacaagcg ggtaacccag tacatcagcc atcatctgac gaaaaatcgg agattgagca
    28621 aacccgcctg tagcacggat ctctgtgagc tgattttcct ggcaaactcc ttttaaaatc
    28681 cgataaaggt tgagacagat cccttctaga atcgccttca tcatttcttt ttctgtatgt
    28741 tgccgctgta aaccgataaa actgccaaac gcatctgcgt cccaaagcgg tgctcgctca
    28801 ccaagtaaat agggatgaaa aatcagacca ttggcacccg ctggcacaaa agcgatagcc
    28861 gaaagcagtg catcatatgg attttctcct ttttctttag cagattgaac gacttcctgt
    28921 aaaaatgtat gccctgccca atcaaagatg ccagcaccat tgctagtcgc tcctcccact
    28981 acccaatgtt tctgatccag aacataacaa aacgtctcca tttctggatg gaactgaggc
    29041 tgagtagtaa taaaacgaag tgctccgctc gttcctacgg tcaaagcggc gatcgtatca
    29101 ccaaaagcac ctaaccctag attagaaagc ggcccgtctg cccctcccaa aacaaatgat
    29161 atatcatttg gcaaacctag ttctgctgct gtttttgatg gtagacctgt tacttgatag
    29221 gtggaaggaa agacttgcgg caattgctct attcgaatgt tcaagtagtc aagcacttct
    29281 tctgcccatt ggaattcatg gatatcaaaa tatccagttc ctgaagctgt tccgtagtcg
    29341 ctgatcagtt cgccaaacaa tctataaaag atatagtctt tgataccgac aaaataagca
    29401 gtttttttga ataattcagg ttggctgttt tgcagccact tgattttgtt caatggtgtc
    29461 attggatgaa tcggcgtccc agtctgccga taaaattctt tgccaagcac ggtatatttc
    29521 agtttttctg cttcgtccgc tgctcggtta tctgcccaag tgatcattcg agataacggt
    29581 gttttctctt catccaacaa tatcaggcca tgcatcgcag aggagaagga gacaaattga
    29641 atgattgtgt ctgaaggcaa ctgtttaatt acttctttta taacggtgca gacaccttga
    29701 aagatcaatt cctgatcttg ttctgccatc ccttgtgttt cttggatcaa agggtagcga
    29761 aaataagcgg atgctgcttc ttgggcttca tctgtgaaag caactgcttt ggtttgtgtg
    29821 gttccgatat ctaatccgat tgaaacgttc attttttcac ctcaaatttt atgtatgtac
    29881 aaaaatttag tttaagcttg cgtatcttgg ttttgaaaag ggggagatgg cgtgttactt
    29941 tattttatca ttggttgctg tatcggatca tttttatgtt taaccgccca gcggattccg
    30001 ttaggtcact ctattattta tcctagatct cattgtgtca aatgctgtcg ttcgctttct
    30061 tggtatgaat tgattccgat tctctcgatc atcgtccaac gatttcgctg ccgctattgc
    30121 aggtgtcggt tgcccttcta ctatttgttg gcagaagcct tatgtggtgg tctttttgcc
    30181 tggttcttta ctttttcttc cgcaagaaat ttttcgacgt tcatatggat gctttcggct
    30241 ttgctatttt cgttgatgga tttattttac ttcatggtcc attctcacac gctattctgt
    30301 tcttgggcaa ttttatggat tttttggttg caggccggcg tcttccaatg gcaaagtgtg
    30361 ctgttaacgt ttatcgtagg cggtgtgtgc cttcgctatg gtcaatccta tctcggagct
    30421 ggcgatactc tgttgatgct ttcttggagt ggtggcttgt ctctagaaga gttgcttcag
    30481 atcttgtttc ttgctagtct gtttggaata tccgtttttt ctgtttactt tatctgtcat
    30541 aaaaaaaagt tggaaaagct gcctttcatt ccttttttga gcagtgcgtt gctgattgtc
    30601 cttcatctta ggtattgaaa aaaggttgtg acaaacgact tgtcacaacc tctgatatat
    30661 agtgttctct taccaaatcc ttgtgacttg gagctaggcg acaggttcac aacctctcca
    30721 ctaaatggtg ttcaatcagc tgaccctcag tattagctca aaatgctggt tttacaacct
    30781 ctgatatacg gtgttccata agtagctttt tcggcttctt atttcttgac gccccaatgt
    30841 cgacgaagga aacctgcacg tccagaacct tctcgataaa gattgtaatg gagcttgttt
    30901 ttcttgtaat aatcctgatg gtaatcttct gcaggataaa attcgcttct tggttcgatt
    30961 tttgtcacga ttggcttatc gaatcgcccg ctgtcttgca atgcttgttt tgaacgctct
    31021 gcaatatttc gttcttcttc atcattatag tagatcactg gacgataaga atcaccgcga
    31081 tcagcgaact gtcctaatgc gtcagtcgga tcggtttgct gccaatagat ttccaccaac
    31141 tgatcataag aaataacttc tggatcatat tcgatctcta ctgcttctgt atgccctgtt
    31201 gtgcctgttg ttacttgttc ataagttgga tttggtacat gcccgccagt ataaccggat
    31261 gtcacagaaa tgatccctgg ctgctcttca aatggtttga ccatacacca aaagcatccg
    31321 cctgcgaaaa ctgctttttt tgtcatataa aatccccctt ttttctcatc ataccttgat
    31381 ttccaaaagg aggataacgt tttgcttgtg acagaaaaaa cggaaactat tatatacgtg
    31441 atacggcttc tgataaatgc caaaacgaac gtacttgatc aaaaacggtt aaatcaggaa
    31501 tcacatattc agcagaagga agatttatgt tttcacctat tcccaataca tgcatgttac
    31561 cagccaaagc tgcttgacaa ccagcttctg aatcttcaat gaccaagcat gcatttggcg
    31621 gtacattcaa ttgctgtgct cctttgagaa aaacttctgg atcaggtttt gcttttgata
    31681 cttgcgtgcc gtcaataagg acatcaaaat aacttgtcaa attcgttttt tctaaaatga
    31741 ggcgtgcatt tttgcttgct gatcccaatc cgatcttgat gtttgttttt ttcaagtact
    31801 caagaacagc tattgcacca ggcaaaacgg atgtttcatc cattttttga atcgcttgaa
    31861 gatatagatt gtttttttgc tcagcaaagg ctgatttctc tttttctgag aatttctgtt
    31921 ccatttttcc aaatgacagc aatcgctcta gtgactcggt tctggatatt cctttcagtt
    31981 gctcgttttc tttctcagtg aatggaatgg acagctggtt agctaaaacc agccacgctt
    32041 cgtaatgata acgagctgtg tctacaagaa cgccatctaa atcgaatagt gctgctttga
    32101 ttttcatcga ttacctcctt gaaaatgaac gacttgaagt aaagatgcga tgtttttagc
    32161 tgtgcgatga agattatcag tggcttgctg taaagcttct tgtaaaggca cagctgcagg
    32221 aaggatcgga aaaacggccg aaatgccaac atctgctgct ttttcgcttc ctttaccaac
    32281 acttccgcaa atagctatca caggaacgcc ctctggtact cttctagcaa tcccaactgg
    32341 cgcttttcct tttagacttt gtccatctag acgtccttcg cctacgatta cgaggtctgc
    32401 ttgctgtacc cgtttatcga actccagtaa atccaatacg tagtcgatgc cagagactat
    32461 tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac tactgcccga
    32521 tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt ggtcagccac
    32581 tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag cgccttcttt
    32641 tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa tcagctcaat
    32701 aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca gtgcttctac
    32761 tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc cgatgccgcc
    32821 atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt gttgcaacaa
    32881 ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt gttggtttag
    32941 tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt gaccgtctga
    33001 agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg tttgccgctt
    33061 caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc ctataggtag
    33121 cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg cttgtgctgc
    33181 ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac gcattatttt
    33241 cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt gacaaaaata
    33301 ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa aacaaatagc
    33361 aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga aggaagtctt
    33421 ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa tataactgct
    33481 agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg cggatctggt
    33541 cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat ttcttggata
    33601 cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat tgttttgttt
    33661 tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg taataagtat
    33721 aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc gctacgggca
    33781 taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa atcaataact
    33841 ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg cactggcaaa
    33901 tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc agcttgatat
    33961 ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt attgtcgata
    34021 tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc atcgactttt
    34081 acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc ctttgtttct
    34141 aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc aaggtctgca
    34201 ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt tttgatattc
    34261 aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca tgggaaaagt
    34321 gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg atcataacgg
    34381 tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg caggataaaa
    34441 atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc cttcgcccct
    34501 acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc agcgaagtcc
    34561 atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg atgccaaaaa
    34621 tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct tttctgggct
    34681 tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag cacgatgatc
    34741 ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc ggtgatccgg
    34801 cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc aatatcaatt
    34861 ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac tcgcagttct
    34921 ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc cgttttcaca
    34981 tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc ctgatggaga
    35041 ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg agcagtttca
    35101 atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc gtatgcaaga
    35161 actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca tctgatgaca
    35221 tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata tacaccagct
    35281 gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct gataccaaaa
    35341 aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa atcaaactgt
    35401 ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga tataaatttt
    35461 ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga gtctggtcac
    35521 cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta actggcgtgg
    35581 tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct ggatacagat
    35641 tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg atattcaaag
    35701 caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg ccaaaaattg
    35761 catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa ttttttttcg
    35821 taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat ccagtaatat
    35881 acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct ccttgaagtt
    35941 ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca ttcgcttcta
    36001 cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct tcgtcagaaa
    36061 aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc tctgccatct
    36121 ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa gcattagctt
    36181 cgagggcagc caatacatgt tcgaagtgaa agcgattggg tgtacagaca ctcacgatat
    36241 cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa aatcctgtac
    36301 gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc aattctaaga
    36361 catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt ccagcgccta
    36421 tgattgctac tttcttcatt tatttctccc tcctttgctt atttacttat tgttattctt
    36481 tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt ttttcaagta
    36541 ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac attcatatac
    36601 catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa aatcaattgc
    36661 gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca catactcttt
    36721 ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat aaaaatcagc
    36781 gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat ccgctaacaa
    36841 attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg tacctgtttc
    36901 ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt ctccttcttt
    36961 gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag ctgctgggat
    37021 aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa tttgacgtac
    37081 accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc cgttacaagc
    37141 tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga tattttctat
    37201 taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa atctaaattt
    37261 ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca ttgttctcct
    37321 cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac ataacgcatg
    37381 taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc acaatccaag
    37441 taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt ccatcttggg
    37501 taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt attgaaatgt
    37561 gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac ataagaaatg
    37621 atcccgccat agccgatccc tcttaaagaa gcttgtaatg cggcagcact gccactcgtt
    37681 tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa accgacatct
    37741 gctttcatag gatctagcgt gtaatcggca ccgtattttt ctgctaattc tctacgctgc
    37801 gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa ttgaatcgca
    37861 atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg aacatgtgct
    37921 tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt tttccattga
    37981 tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat cagcgtttca
    38041 cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac caaactgccc
    38101 gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa taccacaccg
    38161 cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa ttcttcatcg
    38221 ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg agtagcgaaa
    38281 attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc tttgaatccg
    38341 gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt tcttttcttt
    38401 ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag ataacgcata
    38461 cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc cccaaatgta
    38521 gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt cggaggtcga
    38581 caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat aaacgaagac
    38641 agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct ggatccggct
    38701 aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa aataactttt
    38761 tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca gtgctgtatt
    38821 ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga tcaaaatacc
    38881 ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt tgaagcgtag
    38941 ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg aggctatcaa
    39001 ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag cattgaaaat
    39061 atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg ttgcaattgc
    39121 ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca aattgacagc
    39181 taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat ttttcatttc
    39241 aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa caatagaatc
    39301 gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata caaataaacg
    39361 cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata gaacatccag
    39421 atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag tgtcacgatc
    39481 ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag cgttttgtcg
    39541 actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct ccagatagca
    39601 aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg tgcttcgttg
    39661 aaaagctgca acctatctac taacagatca ttgacgattc cataactatt attgaacata
    39721 tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa aatcagtgaa
    39781 cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc taatcctaaa
    39841 gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa agaacgataa
    39901 aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac ggcgtctgag
    39961 ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat tggaatcagc
    40021 actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa ttttaagtca
    40081 gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa tagctatcta
    40141 cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg tgcgtcagct
    40201 aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt tccgatcaca
    40261 cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag tccaaagact
    40321 tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga cttgatgact
    40381 tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg agcaccacca
    40441 ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc tgtgaatgct
    40501 tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc agatactgtg
    40561 ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc tgagttcccc
    40621 gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc tgttccattc
    40681 atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta ttgggataag
    40741 ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac ctcgccttca
    40801 tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg ctcactcatt
    40861 gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc tgtgaaatgt
    40921 tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc ttctgataat
    40981 ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc aatatagctt
    41041 tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc ttctccagct
    41101 tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc catcacttta
    41161 gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc taacgtaacg
    41221 atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat gtctggattt
    41281 tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg ttcgacagaa
    41341 gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact ttcaccacaa
    41401 gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt tttcattttt
    41461 ttccacctct tattttatta ttttcagcca catatattca tacggctgga atatcaactt
    41521 tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat ctccttttgg
    41581 taaaagataa cctgctgatt gattggaaaa attatggata actaagactt tctcttcctt
    41641 cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa tcaaactatt
    41701 cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt caccatatac
    41761 tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca aaaatttttg
    41821 ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat ttcgacttcc
    41881 cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa atgaatgagc
    41941 agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta acgcatctag
    42001 atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct tgtagtttac
    42061 tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt ccggcagaat
    42121 acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa aaaaagttgt
    42181 ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg tattacccga
    42241 acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct gtgcttcatt
    42301 ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct cagtgatcaa
    42361 taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga ttttatgcgt
    42421 ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca cagcgtccaa
    42481 gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca ttttcaataa
    42541 aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg tccatacgtg
    42601 tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc ttggtctagt
    42661 cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt acgtatcatt
    42721 caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc agacaaagtc
    42781 gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc ccaaacggtc
    42841 gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg ggaaaaatgg
    42901 caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt tcttaaatgt
    42961 acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca agaaaatatc
    43021 ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa cactgacttt
    43081 ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa tcgcttgtaa
    43141 aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac gatttgatca
    43201 tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat atacacaaaa
    43261 atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa tatggtctct
    43321 catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga atgtctattg
    43381 tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat tgaaacctac
    43441 tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca gagtattaaa
    43501 taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa tcaaagaatt
    43561 gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg acgcgattct
    43621 agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt cagatgttat
    43681 tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac aaacggcaaa
    43741 aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa tgatccgagg
    43801 aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg atcaatacaa
    43861 tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct actcagtgga
    43921 cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga atggacaccg
    43981 tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc gtctcaatgg
    44041 ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga ttatagacgg
    44101 cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc tacgagaaag
    44161 accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca aagtggcaga
    44221 acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg atcccttatt
    44281 gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc agttaggaat
    44341 caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag aaaaaagaat
    44401 cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa tgtagaacag
    44461 aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt tttcaatcac
    44521 tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc catctgtttt
    44581 agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag ggaagaaaaa
    44641 actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa cacatttacc
    44701 cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata gaataacaga
    44761 catgtctact ggttttgttt tattcgagga attattcaat aatagctagc aaaatattaa
    44821 aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat ctatctttac
    44881 ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg gatacgaacc
    44941 gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt tagacaaacc
    45001 aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc tattttgttt
    45061 gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata ctgtagacat
    45121 tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc ataccagtac
    45181 cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga ggatctttct
    45241 taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat gcagcagata
    45301 agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt cgagaagttg
    45361 caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct gcgatgtcta
    45421 ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact ttacgcagca
    45481 tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc atacggtaaa
    45541 cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga acttgcaaca
    45601 attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc atatcgcctt
    45661 ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct gtttttcctt
    45721 tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg acatcccctg
    45781 ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt tcttggacac
    45841 gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat gtacgcatcg
    45901 tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact gcttctccga
    45961 cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag acaccatgtt
    46021 ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca tcaacgattt
    46081 gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca tttgtttcag
    46141 gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat gattcgatga
    46201 tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg caatcttctt
    46261 cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa cctgaatcgg
    46321 cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag ataaacattt
    46381 ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg attcgaccat
    46441 taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa atgttaccac
    46501 gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct tccatcagtt
    46561 tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca taacgttcgt
    46621 catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt ttatgggcat
    46681 tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct actgtgatac
    46741 cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc ttagaagttt
    46801 cttttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt ttcttcttga
    46861 acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca gcttcaacaa
    46921 agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc agataaggga
    46981 attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc atcgtacgtt
    47041 ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca ccgatacgtg
    47101 aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga tctctgaaga
    47161 ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta cctagaacca
    47221 tatcttgaga aggagtaaca actggtttac catctttagg gttcaagatg ttttgagcag
    47281 ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg tggacagcca
    47341 tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc aaacggattg
    47401 cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga agcgtaggtg
    47461 cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc catacttcgt
    47521 cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt tcgcgttgaa
    47581 caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt ggaagtccac
    47641 attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag tagtcgacac
    47701 gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata tgagaaagag
    47761 atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg ttatcgatca
    47821 atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt ccaggtgcat
    47881 tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg taaagatcat
    47941 tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt aaatctggtg
    48001 gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa gcgcggaatg
    48061 cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt gcagatttca
    48121 attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt aattgtttaa
    48181 tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt ttttcgcgat
    48241 attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt tcgatgacta
    48301 catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct aatacaagac
    48361 ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct gctaattcaa
    48421 tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg catcgatcac
    48481 aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc cagtcttttg
    48541 taggaccaaa aatccgctca cagaacaacc cttcgcgttc aggttttaag gtacgatagt
    48601 taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt tcgggagaag
    48661 ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt tccctccatt
    48721 tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct catagaccgg
    48781 attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct ttttcatctt
    48841 ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg gcaaattttg
    48901 tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga agttcgatct
    48961 ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat tctttcacca
    49021 atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg acgattgctt
    49081 cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt tcttgtaaag
    49141 tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa cgttgtccac
    49201 caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt ccgattgaac
    49261 gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc acaccgacag
    49321 acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt ttcgcatcgc
    49381 tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca ccatcaaata
    49441 ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt aattccaata
    49501 cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata tcgattggtg
    49561 ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca acccctttat
    49621 ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga acgatataaa
    49681 cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga gtaaagattt
    49741 tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta tcgcggactt
    49801 cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat aattctgtta
    49861 ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct gcaccaatac
    49921 ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta gggatttcgc
    49981 gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat tcttcaatat
    50041 ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc gcatcttcat
    50101 agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga gccatttcgc
    50161 cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc tcacccaaat
    50221 gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat tttgttacat
    50281 cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca tcaacgtatt
    50341 caacgacacc atcatgttta cataacaaag cagctcctga gtcatgcgct gatttgtatt
    50401 ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct tgacgctgca
    50461 tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga atacatgctg
    50521 tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta tcaatagata
    50581 cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat gttccatctt
    50641 cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca gctgtcaagt
    50701 agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa ggtgtttcga
    50761 taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac ccgatgtttg
    50821 ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga acgtcacgaa
    50881 cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggcgaaa agacgacgtt
    50941 tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac tgagaagaac
    51001 caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt tgaggtgtca
    51061 atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata cgtgctagcc
    51121 cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga tttcctaagt
    51181 ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag ctcattgaag
    51241 caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg tttccgatca
    51301 cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg acagtcattg
    51361 gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg ccattatcaa
    51421 tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt tcaacgatga
    51481 tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat agacgtgttt
    51541 tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg taacgttttg
    51601 gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt ggttcgcctg
    51661 gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg cttgcgtttt
    51721 tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag atttcaaaga
    51781 tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga atcttacgtg
    51841 tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct agccaagctc
    51901 cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg acttttccat
    51961 ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct gccccgttga
    52021 tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact tcttgtgctt
    52081 tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt gcagagtagt
    52141 ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat tcataatcta
    52201 caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct tcaaacattt
    52261 ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg atcaaatttg
    52321 gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt tttccgtatt
    52381 ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa agactacccg
    52441 atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac attttttgcg
    52501 gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac ttcgtgagct
    52561 tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc tgccttttct
    52621 tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa gtcaacactt
    52681 cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt tatgttttca
    52741 attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt agaaaattca
    52801 ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac acaactgaat
    52861 aacttttact ttagctactt tagataggtt tgtattcatc atgatggata caaacgttaa
    52921 acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa gccacgtaga
    52981 caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc attctacttt
    53041 ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg gattttttgt
    53101 ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga ttaggcgctg
    53161 ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag gcatcagatg
    53221 gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat gctgcctata
    53281 aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat ctaacaatcg
    53341 attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag aaactcgtca
    53401 aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct ttattaaatg
    53461 aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa gcccatttag
    53521 tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg gtgccaatcg
    53581 aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc ggcatcatgt
    53641 ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa gaattgaaaa
    53701 aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc caacaagtga
    53761 cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac acgttcgcaa
    53821 gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc gttgctggcg
    53881 cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac gaaagcttgg
    53941 gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca agtgaacttc
    54001 ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc aaagcattag
    54061 gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc tcatgacaaa
    54121 tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc tacttgcgat
    54181 tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg aaggaagctt
    54241 tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc cattgacagc
    54301 ttgtcttcta ggctttttag ggagtgcggc agcagggctt gtttccggac tgcttcacac
    54361 aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt tatattctgt
    54421 cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa cgatctttca
    54481 ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg gtctgctttt
    54541 tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag gacttgctat
    54601 tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca cagataatat
    54661 gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct ccttgcttgc
    54721 gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa ttggacttgc
    54781 ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc gtctcttgac
    54841 tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg aactgaatgt
    54901 tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc tttctttccc
    54961 gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg tcaaatgaat
    55021 gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat caacgaaaat
    55081 catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac gatcatcggt
    55141 ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt ttctgtcgat
    55201 cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt cgaacgttcg
    55261 aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg tttaacggta
    55321 gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt ccgtggtgtc
    55381 aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt ggggttggaa
    55441 aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc tattacgtta
    55501 ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac tgcagcgctt
    55561 gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac tgagcagaat
    55621 ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg gagtcgactg
    55681 atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa acagcagctc
    55741 acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt aaaagacgat
    55801 caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac gcccaccctc
    55861 ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg tttcttcgaa
    55921 aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct tgatgccatg
    55981 acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct ttccggagaa
    56041 aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat caaagaattg
    56101 gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct gccctctgac
    56161 gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga tttgaaagta
    56221 caaacgatgt tgacttctga atctacgatg aaagatgcca aactggcagc agctcaagga
    56281 gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg acgttttgga
    56341 cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct ccatccagac
    56401 aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg tgtgaaagcc
    56461 attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat ctacatggat
    56521 ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag cggcaggatc
    56581 agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca ttttccagct
    56641 gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt cacaaggaat
    56701 cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc agaaaaagcg
    56761 ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt caatcaaaaa
    56821 ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact gatcgtccaa
    56881 acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc tatccgtacg
    56941 caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct tttttctgaa
    57001 ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc tctagaaaaa
    57061 ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc aatgcaaaaa
    57121 acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc tttttcaatc
    57181 ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt attttgccgt
    57241 aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc gcatagatca
    57301 cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt cctcgtgtga
    57361 ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc agccttctta
    57421 agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct tctatttgat
    57481 tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg ttatctgcta
    57541 tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa gttcctgcta
    57601 aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa gtcattgatt
    57661 tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata gagacgcctt
    57721 caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc atatctaaat
    57781 aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat tctaggatac
    57841 ctacttatat gttactacac acccttattt tttacacttt aacagtcacc atcatcaata
    57901 gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga agcaacaata
    57961 tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac agtttaagac
    58021 taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga aaaatgaaaa
    58081 aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg tatggtgtgg
    58141 ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat gatctaactg
    58201 gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa aataagacaa
    58261 ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca aaaaataatt
    58321 ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata caaaaaactg
    58381 attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat aaatttgtcg
    58441 ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat ggatccaggc
    58501 ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa tatattgatt
    58561 tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg atcactccag
    58621 ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc gatttaactg
    58681 atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta acgatgaata
    58741 cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc atcaacagtt
    58801 cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat catgaaacag
    58861 aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact gatttgacaa
    58921 tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc attactcaaa
    58981 accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc aatcgttggg
    59041 atcctaacga aatatttatc agcgacaatg gtacagatac tacagacaca agcgacagta
    59101 cagatacatc taccacaagt gatagctcag attcatcaac ttcaagcgac agtacagata
    59161 ctacctctac cacaagtgat agcacggata catcagc
//
LOCUS       DAPBCS010000009        58133 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPBCS010000009 DAPBCS010000000
VERSION     DAPBCS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 58133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.009"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            188..562
                     /locus_tag="RD521_000716"
     CDS             188..562
                     /locus_tag="RD521_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7770473.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(676..1305)
                     /locus_tag="RD521_000717"
     CDS             complement(676..1305)
                     /locus_tag="RD521_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7770474.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1439..1511)
                     /locus_tag="RD521_000718"
     tRNA            complement(1439..1511)
                     /locus_tag="RD521_000718"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1476..1478),aa:Thr,seq:ggt)
     gene            1725..1798
                     /locus_tag="RD521_000719"
     tRNA            1725..1798
                     /locus_tag="RD521_000719"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1759..1761,aa:Asn,seq:gtt)
     gene            complement(1936..2100)
                     /locus_tag="RD521_000720"
     CDS             complement(1936..2100)
                     /locus_tag="RD521_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770475.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2395..3723
                     /locus_tag="RD521_000721"
     CDS             2395..3723
                     /locus_tag="RD521_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDT7770476.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3925..4758
                     /locus_tag="RD521_000722"
     CDS             3925..4758
                     /locus_tag="RD521_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDT7770477.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4774..5511
                     /locus_tag="RD521_000723"
     CDS             4774..5511
                     /locus_tag="RD521_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDT7770478.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5666..6634)
                     /locus_tag="RD521_000724"
     CDS             complement(5666..6634)
                     /locus_tag="RD521_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDT7770479.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6866..7699
                     /locus_tag="RD521_000725"
     CDS             6866..7699
                     /locus_tag="RD521_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDT7770480.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7733..8572
                     /locus_tag="RD521_000726"
     CDS             7733..8572
                     /locus_tag="RD521_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7770481.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8598..9158
                     /locus_tag="RD521_000727"
     CDS             8598..9158
                     /locus_tag="RD521_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7770482.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9357..11078
                     /locus_tag="RD521_000728"
     CDS             9357..11078
                     /locus_tag="RD521_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDT7770483.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11243..12385
                     /locus_tag="RD521_000729"
     CDS             11243..12385
                     /locus_tag="RD521_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDT7770484.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12401..13612
                     /gene="thiI"
                     /locus_tag="RD521_000730"
     CDS             12401..13612
                     /gene="thiI"
                     /locus_tag="RD521_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDT7770485.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14185..14832
                     /locus_tag="RD521_000731"
     CDS             14185..14832
                     /locus_tag="RD521_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDT7770486.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14961..15177
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15225..17870
                     /locus_tag="RD521_000732"
     CDS             15225..17870
                     /locus_tag="RD521_000732"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDT7770487.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18180..19493
                     /locus_tag="RD521_000733"
     CDS             18180..19493
                     /locus_tag="RD521_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDT7770488.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19483..20136
                     /locus_tag="RD521_000734"
     CDS             19483..20136
                     /locus_tag="RD521_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7770489.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20191..20862
                     /gene="radC"
                     /locus_tag="RD521_000735"
     CDS             20191..20862
                     /gene="radC"
                     /locus_tag="RD521_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDT7770490.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(20757..22109)
                     /locus_tag="RD521_000736"
     CDS             complement(20757..22109)
                     /locus_tag="RD521_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7770491.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(22175..23464)
                     /locus_tag="RD521_000737"
     CDS             complement(22175..23464)
                     /locus_tag="RD521_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDT7770492.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(23551..24255)
                     /locus_tag="RD521_000738"
     CDS             complement(23551..24255)
                     /locus_tag="RD521_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDT7770493.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            24455..24721
                     /locus_tag="RD521_000739"
     CDS             24455..24721
                     /locus_tag="RD521_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7770494.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            24734..24892
                     /locus_tag="RD521_000740"
     CDS             24734..24892
                     /locus_tag="RD521_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770495.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(24889..25119)
                     /locus_tag="RD521_000741"
     CDS             complement(24889..25119)
                     /locus_tag="RD521_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDT7770496.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            25191..25346
                     /locus_tag="RD521_000742"
     CDS             25191..25346
                     /locus_tag="RD521_000742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770497.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            25503..25658
                     /locus_tag="RD521_000743"
     CDS             25503..25658
                     /locus_tag="RD521_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770498.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(25655..25879)
                     /locus_tag="RD521_000744"
     CDS             complement(25655..25879)
                     /locus_tag="RD521_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDT7770499.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            26146..26481
                     /locus_tag="RD521_000745"
     CDS             26146..26481
                     /locus_tag="RD521_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770500.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            26714..27655
                     /locus_tag="RD521_000746"
     CDS             26714..27655
                     /locus_tag="RD521_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDT7770501.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            27657..28547
                     /locus_tag="RD521_000747"
     CDS             27657..28547
                     /locus_tag="RD521_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDT7770502.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            28590..29390
                     /locus_tag="RD521_000748"
     CDS             28590..29390
                     /locus_tag="RD521_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDT7770503.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            29405..30256
                     /locus_tag="RD521_000749"
     CDS             29405..30256
                     /locus_tag="RD521_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7770504.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            30253..30570
                     /locus_tag="RD521_000750"
     CDS             30253..30570
                     /locus_tag="RD521_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770505.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            30563..30745
                     /locus_tag="RD521_000751"
     CDS             30563..30745
                     /locus_tag="RD521_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770506.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            30747..31217
                     /locus_tag="RD521_000752"
     CDS             30747..31217
                     /locus_tag="RD521_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770507.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            31214..31756
                     /locus_tag="RD521_000753"
     CDS             31214..31756
                     /locus_tag="RD521_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDT7770508.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            31753..32136
                     /locus_tag="RD521_000754"
     CDS             31753..32136
                     /locus_tag="RD521_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770509.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            32133..32363
                     /locus_tag="RD521_000755"
     CDS             32133..32363
                     /locus_tag="RD521_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770510.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            32370..32573
                     /locus_tag="RD521_000756"
     CDS             32370..32573
                     /locus_tag="RD521_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDT7770511.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            32570..32866
                     /locus_tag="RD521_000757"
     CDS             32570..32866
                     /locus_tag="RD521_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770512.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            32943..33356
                     /locus_tag="RD521_000758"
     CDS             32943..33356
                     /locus_tag="RD521_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDT7770513.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            33369..33755
                     /locus_tag="RD521_000759"
     CDS             33369..33755
                     /locus_tag="RD521_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770514.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            34247..34319
                     /locus_tag="RD521_000760"
     tRNA            34247..34319
                     /locus_tag="RD521_000760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34280..34282,aa:Lys,seq:ttt)
     gene            34705..34965
                     /locus_tag="RD521_000761"
     CDS             34705..34965
                     /locus_tag="RD521_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770515.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            35008..35181
                     /locus_tag="RD521_000762"
     CDS             35008..35181
                     /locus_tag="RD521_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770516.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            35570..36403
                     /locus_tag="RD521_000763"
     CDS             35570..36403
                     /locus_tag="RD521_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDT7770517.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            36396..37811
                     /locus_tag="RD521_000764"
     CDS             36396..37811
                     /locus_tag="RD521_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDT7770518.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            37823..39352
                     /locus_tag="RD521_000765"
     CDS             37823..39352
                     /locus_tag="RD521_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7770519.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            39270..40322
                     /locus_tag="RD521_000766"
     CDS             39270..40322
                     /locus_tag="RD521_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDT7770520.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            40324..40641
                     /locus_tag="RD521_000767"
     CDS             40324..40641
                     /locus_tag="RD521_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770521.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            40768..41391
                     /locus_tag="RD521_000768"
     CDS             40768..41391
                     /locus_tag="RD521_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDT7770522.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            41406..42296
                     /locus_tag="RD521_000769"
     CDS             41406..42296
                     /locus_tag="RD521_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDT7770523.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            42319..42534
                     /locus_tag="RD521_000770"
     CDS             42319..42534
                     /locus_tag="RD521_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770524.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            42546..42887
                     /locus_tag="RD521_000771"
     CDS             42546..42887
                     /locus_tag="RD521_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770525.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            42887..43258
                     /locus_tag="RD521_000772"
     CDS             42887..43258
                     /locus_tag="RD521_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770526.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            43251..43646
                     /locus_tag="RD521_000773"
     CDS             43251..43646
                     /locus_tag="RD521_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDT7770527.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            43648..44025
                     /locus_tag="RD521_000774"
     CDS             43648..44025
                     /locus_tag="RD521_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770528.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            44026..44634
                     /locus_tag="RD521_000775"
     CDS             44026..44634
                     /locus_tag="RD521_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7770529.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            44634..44975
                     /locus_tag="RD521_000776"
     CDS             44634..44975
                     /locus_tag="RD521_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770530.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            45217..48006
                     /locus_tag="RD521_000777"
     CDS             45217..48006
                     /locus_tag="RD521_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDT7770531.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            47996..48736
                     /locus_tag="RD521_000778"
     CDS             47996..48736
                     /locus_tag="RD521_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7770532.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            48733..51495
                     /locus_tag="RD521_000779"
     CDS             48733..51495
                     /locus_tag="RD521_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7770533.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            51508..52416
                     /locus_tag="RD521_000780"
     CDS             51508..52416
                     /locus_tag="RD521_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDT7770534.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            52416..53036
                     /locus_tag="RD521_000781"
     CDS             52416..53036
                     /locus_tag="RD521_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770535.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            53040..53489
                     /locus_tag="RD521_000782"
     CDS             53040..53489
                     /locus_tag="RD521_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770536.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            53489..53983
                     /locus_tag="RD521_000783"
     CDS             53489..53983
                     /locus_tag="RD521_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770537.1"
                     /translation="MADIVQLKEDGVAKYLKTHVKAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            53997..54344
                     /locus_tag="RD521_000784"
     CDS             53997..54344
                     /locus_tag="RD521_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770538.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            54337..54477
                     /locus_tag="RD521_000785"
     CDS             54337..54477
                     /locus_tag="RD521_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDT7770539.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            54547..54966
                     /locus_tag="RD521_000786"
     CDS             54547..54966
                     /locus_tag="RD521_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDT7770540.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            54963..55973
                     /locus_tag="RD521_000787"
     CDS             54963..55973
                     /locus_tag="RD521_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7770541.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            56088..57296
                     /locus_tag="RD521_000788"
     CDS             56088..57296
                     /locus_tag="RD521_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770542.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            57280..57450
                     /locus_tag="RD521_000789"
     CDS             57280..57450
                     /locus_tag="RD521_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770543.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
     gene            57705..57778
                     /locus_tag="RD521_000790"
     tRNA            57705..57778
                     /locus_tag="RD521_000790"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57739..57741,aa:Ile,seq:cat)
BASE COUNT        20221 a         8942 c        11877 g        17093 t
ORIGIN      
        1 ttcttctagt ctacacacct taatttttct gtctagttaa ttgtagccta tccaagtcta
       61 cactctgaga tgtaagaaat atcataagat atttcttaca tctttttgtt ttgagcagga
      121 ctttagataa ttttattaat atatgctagc atgcttttgt aagaaaaaga aagagggcgt
      181 attagttatg tatcttaaaa ttacggaaca agcacaagaa agattacaaa aatatattga
      241 tgaaggcgca acagttatat tggatttaga tgatggcgtt ggggaatatt ctaaaatggg
      301 tgtttgctct ttagatacta gttttcgttt acttcttctt gataaagaac agagaaaaaa
      361 tgattataaa ctccaattag atagtgatat tggggatgtt tatataaaag attattcaaa
      421 aatgtacatg gatgaacata tgaccctttc tttagatcca cgtctaggtg tatttactct
      481 tgaaagccca agtggttctt tagacagtca tgttcaatta gtagatctac gaccagaaaa
      541 attctctgga ataaaacaat aactttctct ctattgtcca taaaactagt ctcaatcaag
      601 ctgtattgaa aaaacaagac aacattctta ccatggtaaa taaaaagacg acacattttg
      661 tcgtcttttt ttaaattaac tattttcgat aaaagatcgc atagcttgtg ccattatttc
      721 tggcttttct ctattcaatt catgttttcc gtttttaata aagagtagcc ggccattcgt
      781 tgaaagttta tataaacgtt ttgctgctgg tttattcgca tgatctttgg taccgcataa
      841 aatcaattta ggaagtcgtg atgtacgaag ctgttttttg cagtctaatg tttcaagtga
      901 acgaagtagg gtaagggttt gttttttaga tagacctaat ttttcaaata tcttagaagg
      961 tacgcagtaa aaataaatat tctggaaagt aagcaataag cgattaggag actcaaattg
     1021 tggagcgact aaaacaacct tttttaaata tggagaagga gttgcaagat agtttaaagc
     1081 aataacagcg cctaaagata atccacataa aataaaaggt tcctttatct gattcaattg
     1141 tacttcgatg tatttggtta agtcgtctaa agtagcagtg tctgtgctgt ttaaatgatt
     1201 gaacaatgaa agacttttgc aagaataact attgggtaga taagagatta catctttcca
     1261 tgattgttcg tcttggccta gaccatgcaa aaaaacaatt ttcatattgc tctccttttt
     1321 atagttatca tatagacttt ctttgtacta ttaaaatata aacattcatt tcttacgtca
     1381 taaaataaaa aaacagcgca gacacattgt taaaccaaca tgttgcactg tttaaggaag
     1441 ccacttgccg ggcttgaacc ggcgacctct tccttaccat ggaagcgctc taccagctga
     1501 gctaaagcgg cattatcatt aatacaaata ataggataac gaatattaat gtatttgtca
     1561 acagaatttt ttcgaaaaaa tgtgtttctc taataggaaa acagatttta gaaaaaataa
     1621 aaataacaaa aaatgcgaac ggtttctttg ataatataat aaaaaaattc aaaaaaagta
     1681 ttgcgttatt tagaagaaat tgatattata ttcatgtact tgattccggc atagctcagt
     1741 tggtagtagc gcatgactgt taatcatgat gtcgtaggtt cgagtcctac tgccggagta
     1801 aataaagctt atgcaaagaa aaagaaatcc aatcacttgg gtttcttttt cttttattat
     1861 tatgatgaat caagctttct gtttttgtca cgtatgttgt aatctttacg aaagagtaga
     1921 gttggctact cataatcaac catacttccc atcatcgcta aatacgaaat aaatcagcat
     1981 tcctacacca atcaaagcac aagctgcacc aagaaaaggg atgacattcc caaagttatc
     2041 tgtattcata agtatgccac cacccacaaa caaaaagccg ccagcaatca ggtttttcat
     2101 tttattcatc tcctttctta cgttatagta tttccttctt tcaatgagta ttttgaaagc
     2161 tatcttattg tatgatgttc ttctaggcct cttctttatt atacaggcca ttttcgttat
     2221 ctcatagaaa tattcctttt tatctgaaaa ctattgtata ccgtatgaca tttttcttca
     2281 tgaagttgta agtatattct cattcaaatg aaaatttcat catagaagag attgtatttc
     2341 taaattctta agagtaaaat acgaagtata acttggattt ggaaggatga ttggatgaaa
     2401 gctattacgc ctgatatgac aacaaaattt aacaaacagt ttcaagcaaa tatcaaacag
     2461 caggcattgc agcgggcagt tgtgaagaac ggaattactg cttctgctga aaatgtttca
     2521 gcacatgtgg agaatacacc ggtattttct atagatttgg ctacggggaa agtggctaat
     2581 caaatgcaat cggggcgttg ctggatgttt gctgcactga acacattccg tcataagatg
     2641 ctgaatcaat tccatttaaa agagtttgaa ttatcacaaa attacacttt tttctgggat
     2701 aaatatgaaa aggcaaatta tttctatgaa aacatcattg caacaggaaa agaggcaatc
     2761 gatagccgga aagtagcttt cttactcgct actcctcaac aagatggcgg ccaatgggat
     2821 atgatcgttt cattattcca aaaatatggc gttgtgccta aatcggtgat gccggaaagt
     2881 gcgaatagtt caaattctcg agatttgaat aactacttga acaaattact gcgtaaagat
     2941 gcagtagtgt tacggaaaat ggttgctcaa ggagaaacat tagacgctat tgaagaaaag
     3001 aaagaagaaa tgctagaaag catttataac ttcttagcta tctcattagg aacacctcca
     3061 aaagaattcg attttgagta ccgcgatgaa gaaaagaact atcatcttga ccgaggactg
     3121 acaccacaaa ttttttatga taaatacatc ggtgtaaaat tagatgatta tgttagcgtg
     3181 atcaatgcac caacaaaaga caaaccattc aatcgttctt atacagtaga gatgctagga
     3241 aatgtcgttg gcgggaaaga agtcaaatat ctgaatgttg atatggaaac tttcaaaaaa
     3301 ttagcgattg ctcagcttga agaaggagaa tctgtttggt ttggttgtga tgtaggtcaa
     3361 tcttctacta gaactagtgg catcatggcc ttagatgctt attcaatgga tgatctattt
     3421 gatacggatt ttacaatgac taaagctgaa cgtttagatt ttggagaaag tctgatgacc
     3481 catgcgatgg tattgaccgg tgttgactta gtagacgggc aatcaacaaa atggaaagtc
     3541 gaaaatagct ggggcgaaaa agttggaaag aatggcttct ttgttatgag tgatgcctgg
     3601 atggacgaat atacgtacca aatcgttgta agaaaagatt tattgacgaa agagcaatta
     3661 aatgcgttca atgaagaacc aattgttcta tctccgtggg atccaatggg tgctttagca
     3721 tagacgagta tttcaatgaa gagtatgtag taaagataaa aagagctggc ttaagccagc
     3781 tctttttatc tttaaagtcc attccctgtt ttttatggtc cctgtgctat aattctaaat
     3841 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
     3901 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
     3961 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
     4021 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
     4081 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
     4141 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
     4201 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
     4261 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
     4321 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
     4381 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
     4441 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
     4501 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
     4561 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
     4621 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
     4681 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
     4741 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
     4801 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     4861 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     4921 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     4981 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     5041 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     5101 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     5161 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     5221 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     5281 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     5341 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     5401 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     5461 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     5521 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     5581 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     5641 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     5701 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     5761 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     5821 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     5881 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     5941 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     6001 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     6061 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     6121 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     6181 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     6241 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     6301 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     6361 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     6421 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     6481 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     6541 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     6601 ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta cgaataattt
     6661 tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc agtgaagaat
     6721 aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa agactcaacc
     6781 ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa gcagccaagg
     6841 aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca
     6901 tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt ttctaccctt
     6961 agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg agaattggat
     7021 aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca
     7081 aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt ttttagatgt
     7141 tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat tcccctcatc
     7201 attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg
     7261 ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat ttcttgaacg
     7321 tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg agccacagtt
     7381 gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg ctctttttcg
     7441 attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc gtaaaaaaag
     7501 aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag tcaaagtagt
     7561 tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg gtgatgcggc
     7621 tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac tcaatccaac
     7681 ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa
     7741 agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag cgttggaatc
     7801 aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg tagcaacggg
     7861 gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata tgtatgtcgt
     7921 gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc cttttgaaca
     7981 aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa tcgtttttgg
     8041 tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga tttttatcaa
     8101 gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca
     8161 ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat taaaagaacc
     8221 aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag aacatcgctt
     8281 tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc caaaaggtgg
     8341 ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat
     8401 tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag gaattggagt
     8461 ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa
     8521 tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt agtcaatgaa
     8581 agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa acattccaca
     8641 aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat gaagctcata
     8701 cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact tcacctaata
     8761 aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa gcagaagaaa
     8821 aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta gatgcactga
     8881 cagatatcct ttactttact tatggttctt tttcattact aggaatagtg cctgatcgaa
     8941 tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga aaacctcatt
     9001 atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa tatgctccag
     9061 aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct aagaagaaat
     9121 ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac tatttttgtt
     9181 atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc atgaaaatag
     9241 tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg gatttatgcc
     9301 aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa ccataaatgg
     9361 gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt gtcgtctttt
     9421 tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca
     9481 aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc
     9541 tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat gacatatcta
     9601 cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt
     9661 ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg gaccaaatgg
     9721 agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca
     9781 actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa aaagtcagag
     9841 aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc aaagtgatcg
     9901 aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt gaagcccgcg
     9961 aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg aaaaaaattc
    10021 caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa atcagtgata
    10081 cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt gctgaagata
    10141 tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa acagaagttg
    10201 aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta tatgatatct
    10261 tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg attgcagagt
    10321 atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat cacacatctc
    10381 aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa accgaagtgg
    10441 aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac gaaattcctt
    10501 attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat atcgaaaagc
    10561 aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag
    10621 aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac
    10681 gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact gaccgtattg
    10741 aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc aatcgtttag
    10801 ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat ttagtagatg
    10861 cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat acacacgaag
    10921 aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat cactatcaag
    10981 aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct ttcaaacgca
    11041 tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt agtaaaccag
    11101 caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg aaaattgaaa
    11161 caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa tagaaaagaa
    11221 tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa ccccaatctt
    11281 tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga atccatcaag
    11341 cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa agcagatcgc
    11401 tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa cagaaggaga
    11461 taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc atatgatcat
    11521 ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag aattaggaat
    11581 cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg aattgaaaga
    11641 attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg aagtaggatc
    11701 gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa ttcatttcca
    11761 tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga cagaaagagt
    11821 tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg gtttcattta
    11881 ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag aaagaaatca
    11941 gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt tacgtttatc
    12001 attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag ctctaatcaa
    12061 tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact atgcaccgca
    12121 tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga
    12181 aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata tggcaagcag
    12241 tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc gaatcagctt
    12301 agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca agcagcttta
    12361 taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca gtgaaattat
    12421 ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta tcatgcaact
    12481 agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg
    12541 ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc caaaactagc
    12601 aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag atgtcgaagt
    12661 tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag aaacatttaa
    12721 aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat tgaatcaaac
    12781 attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc gtcaaccaga
    12841 tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa
    12901 gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc tttctggggg
    12961 gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt
    13021 tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa aagatctaac
    13081 agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat ttaccgagat
    13141 tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta ctcgtcgaat
    13201 gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca ttttgaatgg
    13261 tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta tcaatgaagt
    13321 aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa tcattgaagt
    13381 agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt gctgtacgat
    13441 ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc aatatgaaga
    13501 gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga tggaagagat
    13561 acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat aagaggttat
    13621 gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc tccatgtatt
    13681 ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat caagaagtta
    13741 tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga gatcaagcta
    13801 cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag
    13861 tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa
    13921 tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag gttgcgaaaa
    13981 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
    14041 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt gtctcacacc
    14101 cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac taaatatgtg
    14161 aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc aaaagcaact
    14221 gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc aggtataagt
    14281 agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac gatccgcaga
    14341 gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt cccttttctt
    14401 atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc attagtagga
    14461 tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga aaatctaaat
    14521 atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga tggagaaatg
    14581 atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac agtagcaatc
    14641 tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa aggaaatgta
    14701 accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt caatgttcaa
    14761 tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa ttactcagaa
    14821 gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg tcgtaattct
    14881 ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt tgactaaaaa
    14941 aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg cacattcatt
    15001 cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg
    15061 agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc acttgagagg
    15121 caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt cctagaatac
    15181 gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg gaagaaaata
    15241 acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag cattggttag
    15301 aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca atcgtaatcc
    15361 cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg accttgcaag
    15421 atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg cctggtatgg
    15481 accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag caagggattt
    15541 ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt
    15601 atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat tacagtaggg
    15661 aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt gttaccttat
    15721 acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct caagcacgaa
    15781 cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt tatcatatga
    15841 gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt ccagaaacga
    15901 tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa gcgctcatcg
    15961 gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca gatgaatatg
    16021 tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat ccaaacgact
    16081 ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat gatggtacga
    16141 tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg
    16201 tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg
    16261 gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa tggtttgtaa
    16321 aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa gcagtggaat
    16381 tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc catgattggg
    16441 taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac aaagaaacag
    16501 gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta caagatcctg
    16561 atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg ggctggccag
    16621 atacggaaag cgaagattat aagcgatact tccctactag cactttagta accggttatg
    16681 acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt actggcgaac
    16741 gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg cggaagatga
    16801 gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat ggagcagatg
    16861 ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc ttcagctatg
    16921 aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca cgtttcgtta
    16981 tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa aaatcagtgg
    17041 ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact gagctatttg
    17101 atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg gatgattttt
    17161 gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct gtttcaaaac
    17221 aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta ttgcatccaa
    17281 tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga aattctctag
    17341 tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct tctaaaggga
    17401 tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa gtgaatacac
    17461 cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc gatcaatttt
    17521 tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg acgatatcaa
    17581 gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca gaaatcttct
    17641 tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa aaagaactgg
    17701 aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt
    17761 ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat tatttagaga
    17821 agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa
    17881 agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat cgttacagga
    17941 aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata aattttgtcg
    18001 catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga tcatgtatca
    18061 agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct ctttgtcata
    18121 cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg gaattgttga
    18181 tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt tcacgtcctg
    18241 gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga aaagtaaaaa
    18301 cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg agaagcctat
    18361 tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa acgttcaatg
    18421 aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag cttgtaaaaa
    18481 aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt attactgaat
    18541 tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg gacgtagcga
    18601 ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta ccaatgctga
    18661 cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca attgaagaaa
    18721 tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca ggaaatattg
    18781 ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca ccgctttatg
    18841 tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg ggagaagtat
    18901 ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt ttagggcaac
    18961 atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt caattggaaa
    19021 agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga tggccaggaa
    19081 gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat aatacgcatg
    19141 caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa atcaacattt
    19201 tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg cagcaagtac
    19261 caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa ttaagtgaag
    19321 tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc ggattagcag
    19381 atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca ctttatttca
    19441 tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta taatgcagtg
    19501 atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga agccacacaa
    19561 gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa attcttaggt
    19621 gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt tggcaaaaca
    19681 agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt ttcgcaagga
    19741 gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga acatcagatt
    19801 cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt gaagaagaac
    19861 aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg agcaaaacca
    19921 gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa agaaacattg
    19981 attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat cccggtagtc
    20041 atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc tggggttgtt
    20101 tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt tcgtatctta
    20161 ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa agaagtacct
    20221 actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt atcggatcag
    20281 gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat gtcgattgct
    20341 ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac tttgcatgag
    20401 ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa
    20461 ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg taccagctac
    20521 gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca tttggtttgt
    20581 atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg
    20641 aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta ttgtgcagca
    20701 aggatcgttt tagcgcataa tcatccttcc ggaatatata agttagtaca cataaattat
    20761 ataaggaaat ttactgttat attgccatct ggaaatacta ttatgttatc gataattgaa
    20821 cgccataatg catttttttc ttcttccgaa gattctttat aaatttcatc gaattctttt
    20881 gatagaataa attcgacatt ttttatatct attttagctg gctctacttc aatttcattt
    20941 aattctttca ttaaagcttc tcgatctttt ttgtattcct ctaagtctat aacttcattc
    21001 aagtaggctt tcttcaatct atctaattta ttctcaatat ttttcttact atttttcttt
    21061 ttaataatct ttggttgttt ttttgaatag tttatttgta tttgttcaaa tttttgttta
    21121 gtagcttcaa aaatataacg ttctaaattt ttttcgtaaa aagcgcgctt atttgtgcaa
    21181 cgtaatttat tatttcttcg ttttacacat ctataacaag tcctttcgta tatagattct
    21241 gtaccgtctt ttttcttata tcttgcttta atagtttgac cagctactga tccctgacag
    21301 tcagaacatt ttaataaacc tttaaaaatg taatcacgtt ttgcatttgt ttttaaattt
    21361 ttaggtaagg ctttttgaac agcataaaaa agttttttat caataatagg aggacagtaa
    21421 tttttattat ttcttaattc accaatgtat tttttgttcg tcaacatatt tttaacactt
    21481 tgataatctc taacgattcc atactgagag cctaaataat aaacagtttt tcttaaacta
    21541 ccactagaaa gaaagtagtt gaatatatct atcacgatag gagcatcatc atttattact
    21601 aatttcttat tttctataga gtagcctaac ggagttttcc cgcttactac ttcaccttgt
    21661 gcaattttat ttgccataac agctttgatg cgttctgaat caatttgggc ttccaattca
    21721 gcaaaactca ttacttgtgc aatgaaagtt ctgccatacg ctgtagttgt atcataatat
    21781 tgttgagaaa cagcattcca agaaacattg tgtttttcta aaatttcttg agtgtttaaa
    21841 taatgtcgta gatttctaaa ccatctatct aattttgtaa ataaaattag atctatttta
    21901 ttttctttta catcttgtaa tagtttttga aattcatcac gatttatttt ttgaccagaa
    21961 ataccaccgt ctacatattc attaactact ttcaaatcct tgtgttgttc gatatattca
    22021 tagagggttt ctttttgttc tctaagacta tctccatgtt tggcttgttg ttcagttgat
    22081 actctcatat ataatgctac tcgtttcatg ttatctcctt tcgaatgtat gttcttatcg
    22141 ttgtaaaaga aaagcccgaa ggctaatctt tttactacac atctgttaat tctaatgatc
    22201 ttctgtattg ttcagcttga gctagtttag taaaagatac agttccgtta taattttcta
    22261 aaacaacttt ttcaatttca tcaagagata ctttaaagaa ttcttttcgt tgattaactt
    22321 tattgacttc gttttttcta aaatgcgaat gtagttgatt ttctaatgtt ggtgcatctt
    22381 ctgaaaatat cattgcatga acatcaaatt caaaaggtac agaagcgcta ctaagttcct
    22441 taattctatc cataggttct aaccgtctag tcattcctat tttatagaca tcttcaccga
    22501 aagatccgat attactgatg acataaacat agccagctct agtattaagt tctctttgca
    22561 atacatcttc tttgacagtt tctagttctc ttaatttctc ttccagttct ttaattttat
    22621 cagcatagag ttctttttca acatcagcac tagctttttg catgtatgcc attaacttag
    22681 ttatctctga gttaaactgt cgttcttctt tatcaatgcg tttcttttca cgttctattt
    22741 cacgtcttac cttttcttct tcaatcattt gttcttttat tgctttttgc tgttcttttt
    22801 catcgttaac tttaactatg tatgaatagt tgagttctaa ttcttgaagt tttaattcaa
    22861 gtaattcttt tttgaattct gcttcatcgt ttttaaaaag cttgtttatt ttatcaaaag
    22921 ttcttataat tttttctcta gttgaatcta cgttagaaat agtaagttta ttgagaagtc
    22981 cagttatttc tgattctaat ggtataagca ttttcttttt aatattatta gcttctctat
    23041 tattcaaata agaaggaata ttaattgcac cgtctaaaat aaaatccttt tctttaatct
    23101 ttaataaaga aagtttgttt tttatttcgg atgaagaagt ttcatctata ttcggaataa
    23161 ttactgattt atagatagtg accagagatg tgtcatattt ttgattaatt tcatcgtcta
    23221 accgctggct ttcagcatta agaatatcaa ttttgttttc tagcgttgac aagtttttat
    23281 tcaatttact tatgtttttc tctttttcag atataaacgt ttccctagca tttaattctt
    23341 gagaatattc ctttacctta ctctctaagg cagaagtttt atcttttaat tttttagctt
    23401 cagataattt gctactaccc ataattatta aaactattcc aactaaaaac aatagtactc
    23461 ccattcaaaa cgcctcgctt cttttataaa ataattgtca aaatcatata aacccttacc
    23521 gaagttgacg gctccggcat gggaattttt ttaaaataca acttttccga taattctaat
    23581 ctgttctggc gatacaattc tatcttcgta taagtcgttt atagaacgta aaatgattgt
    23641 ttgattttcg taatctttat atatctcttt acatgtaact ccgtcaccac ctaattccac
    23701 aatagcaatt tccccatctt ctacgtccgg ttgttcttta acgaatacga attgtccatt
    23761 cctgataaca ggttccattg aatcgcctat aataggtact gcgaagtcag cagcacttgg
    23821 aactctctca aattcttcct cttcgacaac cgtatcaccg taagtaagtt ctgttggatt
    23881 ggcagccgaa gccgaattgt gaacagttgg gattttttct tttttaggaa aatctaggac
    23941 attttcttgt ttttgttctt ctaatcgatt acttgcaaat tcataggtag caactttatt
    24001 ttcgtcgtta agctgattat atataggaag gatattatat atctgattag atatagataa
    24061 atcaatattc atcaaatcat ctatatcaac attgaatatc ttagctatct catttaaggt
    24121 ttttaatttt ggtgtatatt ttcctttttc ccattcgctt attgaggatg aactttttct
    24181 tcctagtttt tcggctagtt cttgttgatc aatattatac ttttctctaa gaaactttag
    24241 attttcagcg aacatagtaa cacctccttg agttaattgt aacaccgttt cggaaaaaat
    24301 gtaacaggtt tcttagaaaa tattttagtg tttttaaaga agtatttacg caaaatagaa
    24361 atcgatttca ggaaaactga aatatgcact tgacttcgga aaaaccgaag tatataattt
    24421 agtcagaaag ttaaagagag gaggcgaata aaatatggat accgcgaaga aagaaaaata
    24481 tacattgaga caattaagag ctttaaaggg ttattcaaaa gaagaactgt caaggaaaag
    24541 caaagtgaca tcaagaacaa tatttattta cgaaaacgat gtagataaaa tgaggaacgg
    24601 aaagtacgcc actctagaaa aaattgctaa ggcattgggt gttagagttt ccgatatttt
    24661 tttagaccct gattcggaaa aaccgaaatc aataacaggt gaaacaaaac aagtcagcta
    24721 ggaggtgtga atagtggaag agaagaatat aaaagtaccg actcttgaag aatatacaaa
    24781 aattgttgtc gaaacagacg agcagaaccc gaaaacaatc gcagttatta cggcgaatga
    24841 tattgaatca tgcgaaggtt ttcgagttcg aatgacccct agatatgatt aatgttcttt
    24901 atcacgtgga ggtataggat cgtttccaaa actatctttt gattgtattt gtccattctt
    24961 tttttggaca ataaactctg attgctgttt tttagaaatc tttttaccat actcaacagc
    25021 atctgattta ttatcaaata gttttgtatc ttcggaatta ccttcaccac gtacagccca
    25081 tttgccattt ctaggagata cccattgatt tttacccata acaattcacc tcgctttcaa
    25141 gttaattcta ttaagaggtg aaaagaaaac aaactgatgg aggtgagatt gtgagtaaca
    25201 agccaaaaat cactttgaat ggcacaccta atcttcaaag attagcagat gaaatggcta
    25261 gaatacgttc tagacattgg ggagttactg tggtggcaac cataattgaa caaaaagaaa
    25321 agcccacaca gccggcaagc aagtagtggg caccatagaa caataatatt atatcacaca
    25381 ctcgccactt ccgaaaagag gagttgctgc tctcctttca agtttgccaa agtccacgtt
    25441 tcttcatttt ggcaactcct cttcttggag gtggtgacgg cgagagacga aggagggatg
    25501 acatgaagga agaatcaaaa atagggattt tgaatgagaa caattacaca agattaatta
    25561 ttgaaacaga agaaggggaa aaagtagcag aagttacatt gaccgatgca actcctgctg
    25621 acggttatcg tattcgatta actcctaaat atgattaacc ttttggtggg tgtggatcgt
    25681 ttccgtgact atcacggctt tgaatttttc catctctgcc atgaataaat agctcagatc
    25741 cttgattttt agaaatctct ctagcgattt ttactgcttc tgattttgta ttagtatgtg
    25801 cagtcgcttt agaatttcca gcacctttga cattccaacc accattggaa ttaggtacaa
    25861 catgttgatt ttttcccata gttttcacct ccatttctat agaaatattt tatcaaatag
    25921 aaatggagaa attcaataaa cgaaaggagg gatcaaatga cattacaaga gcatcaacac
    25981 ttgatgcgaa aactaaatca ggaataccac gaggaaatga aacctagatt gctaggggaa
    26041 caatggcgtg aacgccagaa gaagtggtta gaaataaaaa agtgactcag ccgtccaaag
    26101 caatgagtca caaaccaaaa attataccta aggagatttt atcacatgaa taagaaaatt
    26161 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
    26221 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
    26281 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
    26341 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
    26401 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
    26461 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
    26521 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
    26581 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
    26641 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc gccaattgat
    26701 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggctcgaaga
    26761 ccgcaaaaga ggtattggtg gctcagatgt tgcaaccgta cttggattaa acaaatacaa
    26821 atctccttat caattgtggc ttgagaaaac gggtcaaatt gaattgaaag atttagaaag
    26881 cgaaccagct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
    26941 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
    27001 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gtaaaacagc
    27061 caatcaattt ctcggaaaag agtgggaagg tgaagaggta ccgcttagct atctctgtca
    27121 agttcagcat tacatgaacg ttttaaacaa agactattgt tatatcgctg ttttgatcgg
    27181 tggacaaaaa ttcatttgga agcggattga acgagatcaa gagctgatcg atacaatcac
    27241 tgaacaatta gtagagtttt gggaaacgaa cgttcttgga ggtatcgagc ctgtaattga
    27301 cggaagtgga gcgactgctg acttcttaaa agaaaaatat gcagatgtag aagaaaatca
    27361 aacagctcta ccatcacgtt ttgatgaact tatcgagcaa aaaagagaac tcaagcggac
    27421 gaaaaaagaa attgaatcat ctattcgtca agtagacaac gagatcatca gtgagctagg
    27481 taaacgtgaa gccagtatcg gtatcacaca aagaaacatt atcagctgga aacttgttcg
    27541 tacaagacgg atgaacacga agaaactagc agagaaatat ccagatgtcg caaatgatga
    27601 agagatttat aacgttactg aatcaagaag gctaaccgaa aaggagatca aataatatgg
    27661 caacaaatga atcgttaaaa aatcaattgg cagaaaagcc acagaaacaa gttgcaccag
    27721 gacagttagg gcttaaagct ctaatgaata caccaacaat gagaaagaaa tttgaagaag
    27781 tacttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
    27841 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
    27901 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
    27961 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
    28021 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
    28081 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
    28141 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
    28201 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
    28261 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
    28321 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
    28381 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
    28441 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
    28501 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
    28561 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
    28621 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg agcttgtgtt
    28681 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
    28741 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
    28801 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
    28861 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
    28921 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
    28981 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
    29041 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
    29101 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
    29161 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
    29221 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
    29281 tgcgagaaaa atacgacaca atggcagcgc aagcaaatcg tgattataaa acaaaacaaa
    29341 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
    29401 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
    29461 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
    29521 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
    29581 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
    29641 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
    29701 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
    29761 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
    29821 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
    29881 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
    29941 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
    30001 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
    30061 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
    30121 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
    30181 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
    30241 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
    30301 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
    30361 aagataagta agctaatcgg tgcagatacg atggatcgat ccaagccatt acccaaactg
    30421 aattttgaat catttacgtt tgagaaatat agccatctga gagaggtagg ctatagtgta
    30481 aatgctattc gaaaagcact tggggtagga agtagtgtat gggcaaaatg gcggaaagaa
    30541 aatgtaaatg aggtaaatca aaatgattaa gctaataggc aaagagacgc tcaaggttta
    30601 ctatgtagcg gaaacaaagg cagaagtcag ccgttggcta ttgaaaatgt ttaataagac
    30661 tagagccaat gcttcagtaa gtcagaaagc aagtgtatca gaaacaggca tgcctgaagc
    30721 aatgtggatt tcaaggagga actgagatgg caatgatacc gaagtttaga gcgtgggata
    30781 gagaaaaaga cagaatgatt tatcctagca cgacaggagt ttgttttgaa atgaatgatg
    30841 atggtataag tgtgcttgat attgatgtag attatcccaa tgactatggt tttcccaaaa
    30901 tagcttcaat cctcatgcaa tctaccactc tgaaggataa gaatggtgtg gagatatttg
    30961 agggggatat agcgcttatc actcataaat caacgaacta cgccgatact tattggcaca
    31021 gttacgtaga agtttttaga gcaaaaaacg gagcctacag aattcgcggt gaacacatct
    31081 acgaaacaga attatatagt aatagaaaac aattgacggt agcggggaat gtttataatt
    31141 caccagaact tttgaagaga gatcgattta tgactactac cgaaaatcca gagttattga
    31201 aggaggaaca gcgatgaata aacaggaatt gattgataaa tatactgcag aaatatctag
    31261 attaagacct tattgcccta atagacattt aattagcgat caacttaaat atggtcttta
    31321 taaagaaatc ctagaagatt taaaacaact agacgaaccg cagaaaccag cggtgcctaa
    31381 gtttgtggcg gaatggatag aatacgctaa aaagaaaggc gatagtctag ctatttcatt
    31441 caagccgtgg aacctctacg gtgttgagta tagcaagact gatagatgga ttgaagataa
    31501 tcaaggaacg tttgctcgtg cttggctaat tggctacgag gtcgagaaag agccgttgta
    31561 ttatgtgaag ttgccagttg tgtattttaa tcattgggat ttagagacgt atctaatgaa
    31621 agatgataga ggaaatataa caattgcaga caacaacgat tttgatgata tgaaatttac
    31681 ggaatcagaa ataaaagcaa ttgatgaaag atactggcca tttgctgtgc cagtggaaga
    31741 ggtggcggaa ggatgagcaa tcttaaagtt ggagatattg ttttttacaa aggtgacgaa
    31801 ggcatcatcc gagaagttat gacggatgga aaagaaaatt tttatagggt agatgtcagc
    31861 agaaaatcta tactctacct gtatgaagac gaattagatt taggtactca aaaactcaac
    31921 gacaaccaga aaatggtgtt tgattatctt aaatcaaacg ttgaacaagg tggcaaatca
    31981 gttatgtatt cgatattcct tctaggtgat tgggactctc gaattggagc agcaagaaac
    32041 gttgatatag cttactgtgg attaaatggt aagcaggaag cagaagtcct atcagcattc
    32101 gctcaatggg gattagaaca ggaggaagcg gaatgaaata cgaaatacca ctaagtgaag
    32161 cagacgtcca gtcaattatt aacggtcggg aggttaataa aaaacttcct gatggtactg
    32221 aattagtcat cagacaaagt tatttgaaag atatggcagc tccagtatta attgatcgtt
    32281 ttaacgtgac tgattctgtg gtagagaacc atttaaaaga atttcgatca agtatagacg
    32341 acactttcag attagggagt tgattgacaa tgaacaacag acatcgcaga ataacaaaac
    32401 taagaaaaca ggaactgaat gtactaaaga caaagtttga aaaagaatat ggaatttcag
    32461 cagaagaaac atataaagtg gcaagtcagt gtgttgctga tgcgagtaat gctattcgta
    32521 agtttgggat ttcgatatta aatgatgatc gtaaatggga ggaaatgaga tgaaactaaa
    32581 agacggattt tacgctagta gtcacggcat cggcggttta atgctagata tgccgacaaa
    32641 gaatcctaaa acacgtaaga aaccaaaagt caaagtcggt gacatggttc gctgcgaagc
    32701 agaagggttc atctatccat ttcgtggata tgtagaacac gtctataatc actcagcaat
    32761 cattcgtatt gaaaacacga tggaatgtga taaatggaca gctaaaagca aagagaattt
    32821 agcagtggtg agattggtgg atattgaact aatcaatgac aaataaaaaa gccggatcgc
    32881 tccgactgat tcaataaatt ccacaagttt attatatcac ataaaagggg cggtttgact
    32941 tgatgcaatt gttacgagag gtagctttca aacagacaag atgtaatgcg agagatgtgc
    33001 tgaagaactt tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
    33061 cgattattac ggacatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
    33121 ttcagatgat ggatatagaa gcagagagag acgcgatttt agcggctttg atggcgctta
    33181 gtctgattag tcgtcagata ctctactaca gcttttgtgt gccagatagc ttctcaaact
    33241 acaggattag ccgtgaagtg ggttattcag aaagaagcat acaacggatg aagtcggaag
    33301 ctctgataga gtttgcagaa gcatataaac atgggaaaat aattgcttat aaataggagg
    33361 actatataat gtggaaagat tatgtatcat tgaaagagtt gaaaaaagat cttattttca
    33421 aaaaaatcgt agaatggtca gagagtgaat taattttaga agatggaacc aaaatggaag
    33481 ttgtatgtag cgaatcagat tgctgcgcat gggccgaagg tgaattcaaa aatgtaaagc
    33541 ttgatgcagt gattacagat ataaagattt ttgataaagg taaccgtctc tataatggcg
    33601 atggacatag ttcttatgcc gaagtcgttg tttatcataa tcgaaatgag attgctaaag
    33661 cggaatgtac agcaaacgat gggaatggag gctactatta cagtgtctgt gctttaaaag
    33721 tcaaagaaaa actttgtgta gtgactgatg cataaaaaat ggcggttttt tggcggaatg
    33781 atggcggttt ttagctattt accagtgata ttatggtagt gtcgaaagat tagtgatagg
    33841 tctgagacaa aataaaatgt aagggaggaa atctccctca tcgtttaatt aagcttcgat
    33901 agacagcagc ggaaatatta agaataagga tgtgaatttt aactccttct aaattgttct
    33961 tattatctat catccgttgc tgtctattgt tattatgtca ctgtggcgga aagggtagac
    34021 gcttaaaaat aaggtcaata cgtcgaggga tagccttaac gttttatgat ttgaccatgc
    34081 aaggttcgat tccttgccag cgacttagac gaaaggtagc ttaaatatta accacatcaa
    34141 catcgtcagc gagttgataa aggtaactag cagactctag gatttaagcc agtcttaccg
    34201 attagtctgt tatagtagca agcttgctag aggtagctcc tctggttgac gtgcagctca
    34261 gttggtagag cgcctgactt ttaatcagga ggtcgccggt tcgaatccgg tcacgttaat
    34321 aaagaaccta cggaaacaat caatcttatc ggatgccgat tggttggctg aattaagcag
    34381 tgtgtctgtg aatcagttaa taggcataaa atactagcac agacgtgtgc cactctcagg
    34441 tgtaggttag gagagaaaca ttagcaaccg agggttggaa atgggcgctc aaagtacacg
    34501 agcaaggcga ggtcgatagt aatcgatgga atcggtgtag gttgctatta cataattgat
    34561 tgggttagat tgagttttgg gattcggtac aaatgaatcg tcaaatgact caagcacagg
    34621 atcggaaacg tccctgcctg tgcattacat attagatcac tcgttgagtg gtctttttat
    34681 tttagaaaag agaggatttt gaaaatgaat catgagaagt ttatcgaaaa atgcaaggct
    34741 attgtacgtg aaagaattga gaatgagatt gctgacctaa gtggagcagt acctgagttt
    34801 agtgttttca tagtctggtc atgcaaaaca ctacagaata gcaaagcatt agtcagcgct
    34861 agcttaaaag gagcaccgta ttttgaaatt acgttgaacg gggacaaagg tgaaatctat
    34921 gtagatactt atctcaaaaa atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt
    34981 ttctttacat aaaggaggaa tgatctcatg aaacaatata ccgctaaaga tttcgaggaa
    35041 atgaagcgat taaagaagga ctatgaagaa gttgatatgg agctaactgt tggagtcatt
    35101 caacgaagac tgcgggtcgg attagagaca gcaaaggcta tttacaatga tctaaatgct
    35161 attgaagaga agaatggcta atgaggaact actggtatat atcgctaact aatgaatatc
    35221 ctcgaaccat tgatgattgt tcagtgcgtg ttgtgcgttc tgtacaaatc aaagggaagt
    35281 actctattgt cgaaatgggg agagaagcag aaccatgtga gattgatgcg tgcaagctcg
    35341 tttattgcgg tcatggtttc tatgatgaac caaacattca aaataatatt aacaagaatt
    35401 tgagggatta gaatgcaaga aatggcttaa aaatctccaa tcaggacaaa tcaagttaga
    35461 caaagtttcc gatttaaaga tattaattga agcagatcta atgttgaaag atattgaaaa
    35521 ttagaaaaca aaactcaacc taagaagatt gcgaggtggt gtgtattgaa tggcaagaca
    35581 acgtgatcca agacgtgatg aagccaaaag aatttggtta gaatccaacg gagaaaagca
    35641 gttaaaggaa attgcatctg aattaaatgt ttcagattct caggttagaa aatggaaatc
    35701 gcaagacaaa tggagcgctg aattgaaaag taacgttacc aatggcaaaa gtaacgttac
    35761 taatcaaggt ggcgctccta ttggtaatca aaatgctaaa ggtaacaaag gaaatagccg
    35821 agcttctccg ccagtgggta ataaaaacgc tttgaaaaca ggcgagtatg aaaccatatt
    35881 ttttgagaca ctaagtgatg aagagaagga catctattct agtctgaatg ataatccttc
    35941 ttttgttttg tctgaagaaa tacgtctact taagataaga caatttcgta tgatgaagag
    36001 aatccaacaa gctgaagctg gactaaatga tgaagaagtc gaacgattgc agcagctaag
    36061 aaagattaaa aatccgattg aaaaaaatgg taaaaagcta gaaatcaagc gtgaggttat
    36121 gcaagatgtg cagattagca gaaaaaaaca tcgcaaaatt gatgatattc tttcaattga
    36181 agattcattg actcggatta gcaaccagtt agctaaagcc atcaagcaaa tgaatgaact
    36241 ttatatgaat gaatacagaa ctgatttaat taaagctcag actgataaga tccaagctga
    36301 gacaaatgaa attggcggaa ataattcagg tgaagaaata gaagaatgga aacaggcagt
    36361 tttaaatgcc gcaaacaaac gggcggtgaa agaaaatgaa taatgaattt attccttttg
    36421 ctgatattgg ttctgccatt gattattact atgataaacc agtagctttt tgccaagata
    36481 ttttgcattt gaatcctgat gaatggcaag aaaatgtttt aaatgattta gctgaatttt
    36541 caaaggtttc tgttcgttct ggtcaaggag ttggaaaaac agcattagaa gcaggagcaa
    36601 tactttggtt cttaacgtgt cgaccctacg ctaaagtaat agcaacagct ccgacaatga
    36661 agcaacttta cgatgtactt tgggcagagg tagctaaatg gttaaatgat agcttgatca
    36721 aaaacttact gaagtggaca aagaccaaaa tttatatggt tggtgattca gagcgttggt
    36781 ttgctacggc tagaacagcg actaaaccag aaaatatgca aggttttcac gaggaccata
    36841 tgttgattgt ggtagatgaa gcttctggtg tgtctgatcc aattatggaa gctattcttg
    36901 gtacgctatc aggttttgat aataagctgt tgatgtgtgg aaaccccaat aatattgaag
    36961 gtgtttttta cgattcccac aattcagacc gtgataaata cagagttcat aaagtatcaa
    37021 gctatgatag taaacgtaca aacaaagaca atatagaaat gattcttaaa aaatatggaa
    37081 aagaaagtga tgttgctcgt gtccgtattt ttggagaatt tcccaaaggt gcgttggatt
    37141 catttatcag tcttgaaacg gttgaattgg ctacagaaaa acaaattagt gattctttag
    37201 tcaataaaac aacggttgct catattggtg ttgacgtagc tcgatatggt gatgattcta
    37261 cgattctctt tcctagaatt gctaccaggg cattggagta tgagaagtat tcaaaacgta
    37321 gcaccatgga aacaacagga tatgtcatca acatggccaa gaatctaatg agtcaatatc
    37381 cgagtattga taaagtgatg attaaagtcg atgacactgg tgtcggaggt ggtgtaaccg
    37441 accgcctaga agaacttata gaagacaaac attatccttt tgaggtgttt ggagtgaata
    37501 acggttcaac atcagaagac gatttttacg ataatttagg tactcaacta tggggaaaca
    37561 tcaaggaaat gttagaagaa aatatgacag caaatcttaa cggagaacag cctgttattg
    37621 aattgccttc tgatagttcg ttaatcaaag aattaagtac tcgcaaattc aaaatgacaa
    37681 gtagaagtcg tatacgttta gaaagtaaag atgatatgaa aaagcgaaat attggtagtc
    37741 ccgatattgc tgacgcactg gctttagcgt tttatgagcc accaagtcac tatcaattta
    37801 ttcaatttta ggaggtgagc ttttgattag tacagtatta tctttagaga gatataagaa
    37861 gctacgtgtt aaatatgcaa cacaaattga agatggaatg tttgatccga atggttttat
    37921 agaagatatg aaaccatttt ttgctgatcg cgaaagaaaa tatctagctt atactagcga
    37981 aaagaatgaa atagataata gaccaaagcc taacactgat attgtaaaag ttaataataa
    38041 acttcatgct ggtatgtatt caattgtcgt agaccaagca gtcaatcatt ttactggtat
    38101 acctatcaaa tgggattacg atgtatcgga acaaaaaaga acactcattc aaagattaaa
    38161 agataaattc ttaaaaaatg atatagaact tccgacagtt ccagaagctt ttaacaaatt
    38221 aacaagcaat cttgattcca tgagattcgc aatgcttgat tctgaaactg caacttttca
    38281 aggagcttgt ggagtagctt tccgattgtt agagcctgtg gaagaagatg atggttggaa
    38341 gttaagagca agtaatattg aaccttggag agcagaaaga tatggaaatg ccggaatcta
    38401 tatcaaagaa aagtatgact cttaccagaa aaaatttttt caagaaatga aagttatgac
    38461 gagaaataaa attctgacat atgcttgcta tggtgatttg aattttgtta caagcggaac
    38521 gttcaaaaaa attgatgaga ccgataaccc tttggggacg attaccttgg cagaatttaa
    38581 aaataatacg aatcgttatt gtgattttga agtagctgag gaaattggag atgcaattga
    38641 tcgtgctttg tctgatcaac aaaacgaaat cgaacaattt aagcttgcgt acatgcttgt
    38701 tactggcaca acaatgagca aaggcactgc taaagaaatg atgaatcagc tagggattat
    38761 caacttaaaa gaccctactg caaaagctga atatgtcaca aaaaatttag caaaagattt
    38821 caatgaatat catatggatc tattgaaaaa acagttttac accatttgta aagcaatcga
    38881 ttttaacgat gaggtgttta aatcaaacag ttctggagaa gctcgcaagt ggcaaatcat
    38941 tagcctagaa gcgaaaacaa atactaaaga gcaatatttt agagaaggat tgaaagaatg
    39001 cgcagaaaca attgctgctt ttcttaagtt tcatgacaaa gtagaaattg agcctgaaaa
    39061 aattattttt actttctcta gatctttgcc aactgattta agttatttag ctgaagcttt
    39121 acctaaactt gcaccatacg tatccaaacg aactattcag agccaaattc catttgtaac
    39181 agatgttgat tatgaaaatg aaatgatgga attggaaagt ggaagtgctt atccagatag
    39241 tgaatacaat tttggcggag gtggcaatag tgacgataga aacgaagtat tggaccaaac
    39301 gtcgagaact ggaggatcaa gcaaggctca aacaagaaaa tcagacactt aaaaaattaa
    39361 ctagtgtatt tcctgaagca ctgaaagaga tacaagcaaa actattatca caagctgact
    39421 tacacaatat cacttatcca gaaatgatgg agttttatag tacaagtaat cagaaaaaat
    39481 atcgtgaata tgtggaaaaa aattataagt cattaaaaat gtatgatgca aaatacaaag
    39541 agtttatcga tgaatttttt ccaccatttg actatgcaaa agtcaatcgc ttattacaaa
    39601 tacgatcaga tgtatttaaa attcttgcag aatatgcgat ggatgcagat gtgaatcaat
    39661 atttttctga tcgcttagag gaaattcttc aaagaacata ttcttctaat gctaatgttt
    39721 ttgttcaact tttaaacgtt gatataccaa attatttacc agaaaatgaa attaaacatt
    39781 atttgaacta tccatggtca ggaaagacat tttcaagaag actttgggga aatatttcat
    39841 cgcttgaaca gaaactctct aatgctattg taaaaagtgt tgctagtgga gaaggcgtta
    39901 tacacgcatt aaacaccatg agattagact cagaaatttg tgacatgttt aagttagaag
    39961 aatcaaagta taacaaagcg atagaaaatc tcgttcgaac ggagtatgca aaatttgcac
    40021 aagatggtat tgaaaaatca tatttagaaa caggtattga ggaatacaac gtattgactg
    40081 caaaagatga gagagtttgc cggatttgtg gaggaaaggc aagtaagaat ccctataaac
    40141 tgaaagatgc tgtcataggt gaaaatcgag cacctttcca tagtcgttgt agatgtacgg
    40201 atgttcctaa tttaccaaaa ttaggaaagg atattgatga agaatatgac cgtttatttg
    40261 gcgatttatt agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt
    40321 agaatgaaag atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa
    40381 gcctacaaaa cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat
    40441 ggcaattatg cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca
    40501 gttgataaga ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta
    40561 aaagaaacag ttgattggta tgaaaagatg ggatgtattg ttgttagaac tgactacaaa
    40621 gaaggaaagt ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca
    40681 atactgggct tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt
    40741 gaaaacgaat cgtggggcga aaggagaatg agcatgaaac acaaatcatt aatgccaatg
    40801 aatttgcaat actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct
    40861 tttgtggaat ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc
    40921 gacaaacaat tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc
    40981 aaggaatatg aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta
    41041 caagctgaat ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt
    41101 gcagaacaaa ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt
    41161 atgctaaata atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt
    41221 acaagtgtct tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg
    41281 aagtctgcat ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca
    41341 ttaacaaatc cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa
    41401 aaattatgaa aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta
    41461 atgctatgca agtaccaaat acaccttttt taagctattt atttggtgct ggaaaaacag
    41521 agccagcaaa ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt
    41581 ctgaaaaact tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca
    41641 actatactga aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg
    41701 tgaatggagt cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa
    41761 ttgatttgaa ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc
    41821 gtcgaatgaa tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg
    41881 cgggtgcagt aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt
    41941 atatgggaga aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt
    42001 tagatggaaa atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa
    42061 ttggaaatat cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa
    42121 aaggaacaat tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga
    42181 gagcagaaga acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact
    42241 ctcttgaata caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg
    42301 gagttagtac tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa
    42361 aaagatgaag tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat
    42421 cctaaaactc gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat
    42481 gataaagaag caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg
    42541 atctgatgga tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag
    42601 atttgagtga agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat
    42661 acaacacttg taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt
    42721 tgtataaaat aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata
    42781 caacgattag ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg
    42841 atttgatacg aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga
    42901 tattttagaa actacttatg aagatagttg tattattgaa agactgacgg acattgaaga
    42961 ttctaataca aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg
    43021 cgctctttca caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa
    43081 tgtttcaact gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga
    43141 tcgaataaca ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc
    43201 tttttactat cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc
    43261 tcaaatatga atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg
    43321 ctcaaaaaga aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta
    43381 aaccattaac tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta
    43441 gaaaactatc aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat
    43501 atgtagaaga aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa
    43561 ctcgtgcaac gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa
    43621 taaaaatcgt taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc
    43681 aaattaaaag aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact
    43741 aaagattttt atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat
    43801 attcaaaatg tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat
    43861 ttaattaatg aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag
    43921 caaatattat ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt
    43981 tttacattag aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga
    44041 agtaaaaccg gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca
    44101 gggaattttc cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca
    44161 acagctaatt atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca
    44221 gcttttggtg gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct
    44281 acaattactg gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg
    44341 caagatggtg cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc
    44401 ttttataaag gacagtttgc tctagattca gatgaaacat caacgattga aggaagtaaa
    44461 gtaagtttta ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat
    44521 atgtattcag ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt
    44581 acaaaagtag caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa
    44641 aatatcaaac aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac
    44701 aatttaaatc tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg
    44761 aagaaatgca aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt
    44821 catttattgc agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa
    44881 ttgatgctcg tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt
    44941 ttgatgaaac ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt
    45001 taagtattcg attacttacc gagaattaga tataaagacg caattactcg aagcaggttg
    45061 gacgttacca gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag
    45121 agatgcggta aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga
    45181 ggtgatactt tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt
    45241 actgccggta tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga
    45301 aaaagtagaa atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt
    45361 ctagctagcg gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata
    45421 agtggagcgg ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg
    45481 ggatttagta attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt
    45541 attcaaggac ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct
    45601 tctacaggtg atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt
    45661 ggtttcggtg gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc
    45721 tcaaacggaa aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct
    45781 actctttctg ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg
    45841 tctcaaggta agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa
    45901 ggtggcggag gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa
    45961 acctctatac aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga
    46021 ttaaataaag cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg
    46081 ggcagttcaa tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca
    46141 tttttaagta atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg
    46201 ccctttcttg ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata
    46261 ccagcaataa taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca
    46321 ttaaagatta tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc
    46381 gttggtgcaa tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag
    46441 gttatatcta tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag
    46501 gactggggaa ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt
    46561 gtcaaagatg cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag
    46621 gatggggcca caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt
    46681 gtaaaaaatg catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa
    46741 gattcagcca gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca
    46801 ttgattacag gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg
    46861 aataatttag ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg
    46921 ccggttttgt ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg
    46981 attgctgttt ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat
    47041 atatttgtaa gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc
    47101 aagcttacat tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta
    47161 aacataaagt atttttttat taatctttgg attgatataa aatattttgc aattcagaaa
    47221 tggattgaat tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt
    47281 actacttgga ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg
    47341 gcatacaata cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag
    47401 gactctttct ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat
    47461 ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg
    47521 gaagtcaatt tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa
    47581 gaaaagtgga atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg
    47641 attaaaggag ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa
    47701 aatattgttc aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc
    47761 atgacaaatt tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta
    47821 ccaattacac aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat
    47881 ttaaatgttt atggagattt acctgattca atggttcgga agattaccac aaaaattaaa
    47941 acagaattga caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca
    48001 acttaagaga ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat
    48061 tcaaaacagg cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg
    48121 aaatgattct atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg
    48181 ttactataaa gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg
    48241 gttagatatg tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca
    48301 agctgtagta atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc
    48361 ttttgaattt acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat
    48421 tcgacaaaca gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat
    48481 taaattgagt ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa
    48541 atcgttggac agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga
    48601 tggtaattta gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc
    48661 tggaaaaaat gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg
    48721 gtggagaaaa gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat
    48781 ggcttaggaa ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa
    48841 tatgaattag agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac
    48901 tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat
    48961 aaagatatag atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga
    49021 cttggaagac gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata
    49081 tcaattattg aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg
    49141 gccgtttcta gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa
    49201 ggatcattgc ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg
    49261 ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt
    49321 ttaaaggtca aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct
    49381 caaagtgagg taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt
    49441 aataactatc ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa
    49501 gacattaaga gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt
    49561 gataagccta aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg
    49621 aaagaatttg cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa
    49681 aaatataata tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa
    49741 aaaactttga aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta
    49801 actattcagg ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat
    49861 tatgtttctt caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca
    49921 aaagaaaaac cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa
    49981 gtactaagaa tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt
    50041 acttcagctt ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc
    50101 aaagcagata tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat
    50161 ggactactcc aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg
    50221 gaattttgga attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat
    50281 ccttttcctg gggcttctac tccaacccct attgaagata attctttagt tattcgtaca
    50341 aatggagacg gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta
    50401 ggaaatggta aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa
    50461 ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt
    50521 gggtatccta gtgaattaaa aacagatgca gaaaagagag cttggagaat ttacgatatt
    50581 ttgtgtaata acggatttac gaaacaatct gcatgtggaa tattggggaa cattcaacaa
    50641 gaaacaggag gaacttttga tcctgatact gttcaaatag gtggaccagc atacggatta
    50701 gttcagtggg atggttcttc atatcctctt gttggtccag caacatggga tggaaaagtt
    50761 tatgttcaaa acttatttaa tgctgcaggt attaaagaac caataacgag cttagatgca
    50821 caagttcgat tgcttatttg gacatttaca aatggacaat ggatgggcgt agtacaacct
    50881 acgacggttg atggatttaa ggcctgtact gatccaagac aagcagcata tgcttttgaa
    50941 cgaaactatg aacgtccggc agcgacacat cctgaacgtc aagattatgc agttaactgg
    51001 tataacaaat ttaaagattt aaaaccagga ggagctactg gagaagcagg actaaaacat
    51061 ttagagtctt tgattggaca acgaattggc aatggtcagt gttatggctt gtctgcagaa
    51121 tattcaggat atctgggtgg ttgtggcatg ggtgctggaa caaaatatgg tttaactcat
    51181 gtgataggaa atacttctgc agcatctgat attggtattg cctatgattg gtctgctgtt
    51241 ggttggaaag tgattcaaaa ccctagatat gatcaattag tagttggtgc aattattaat
    51301 tgggcaagag gtggacaagt gggttcatgg tttgcagacg gaacttatgg acatactggc
    51361 gttatccgag gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt
    51421 atgatttgtg gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc
    51481 ataccaccaa aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca
    51541 gaaccctata attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt
    51601 atggaagcta caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat
    51661 tttgaatcag ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct
    51721 aaaaaaggga aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa
    51781 acagccaatt tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc
    51841 tattttgtca tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca
    51901 attgaagatt taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg
    51961 gactggatga atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt
    52021 ttccaagatt ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag
    52081 gatattttga agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt
    52141 gtagatcttc aaggtgtacg ccatgaatcg atttctgaac gtttcctatc cgatctaaat
    52201 tatttgtatc aaaaaatgaa agcagcactt ttcacgattg aatacggtga aattgaagtg
    52261 actgatattc ttcaagacga tctcttttca gataatcacg aagtcgaaaa agttggaact
    52321 gtagaattcc cgattgaaga aggagccttg atcatcgcaa ccgttgatga tccaaagcaa
    52381 aatgtgttca ctcttgagaa agtcggggtg atctaatggc taaaactaaa cgaatgatgg
    52441 aaacggatga aaaaacaggt gtacaacgcc aattctttcc aatcacacat gcttccgcag
    52501 ttcttgggtt agaagaaata atagcaggag aagcaacagt tttatctgtt aatggaaaaa
    52561 ttggagccgt cgtcattact aaagaagatt tgggattaga gaatgttctc acagaattac
    52621 cctatgcaag tgaagaagat gacggcatta tcacggcaga aatgtatcaa aaaattttaa
    52681 acagtggaga aggtgactac gtgttgccag ttgccactat cgaccgtttg ggtggcataa
    52741 aagttggtga attattgacg attgatgaga cagggaaagt ctctgcagtc agacaatctg
    52801 atgtcaattt ttcgctggag ttaaaagaaa aactcgattc actgaaaaat tatactgctg
    52861 gagaaaacat cactattgat gaagatggaa caattagttc tacaggtggt agtgggactg
    52921 gtggtgtcaa tcagtcatat gtagatcaaa aattccaaga agctgtaaat caagcagaaa
    52981 attacacaaa cgaaagaatt ccaaatttta cttttgaaaa aattggggag gtatagagaa
    53041 tgacagatat cgtaaaaata aaacaaagca atgtacaggt ttatccacaa actcattgga
    53101 atgctataga aggcaaacca acaacggtga agggagataa aggcgatcct ggccaagcag
    53161 caacaattac cataggaacc gtttctagtg gttcaaccgc ttctgttacg aatgtaggaa
    53221 catcatctgc agctagattt aattttgttt taccaaaggg agataaaggt gatcctggaa
    53281 taaatgccac aactacagct gtagctacaa ctacagcaaa tgggttgatg tcgtccacag
    53341 ataaaactaa gttggatgga attgcagctg gagcacagaa aaatccaggt aatgctacaa
    53401 ctacgacagc aggtttaatg tcagcaaccg ataaagtgaa acttgatgga ttagccaata
    53461 ttacatttga gaaagtgggg acggtttaat ggctgatatt gttcaattaa aagaagacgg
    53521 agttgctaaa tacttaaaaa cgcatgtaaa agctattgat ggtaaagaag cattggtgca
    53581 gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg taacaatcaa
    53641 taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc tggttgtcac
    53701 taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt ctatatattt
    53761 tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa ctgttccaga
    53821 aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc gttctacttt
    53881 gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa gaaattcaag
    53941 ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga aatctaatga
    54001 aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg aacaatcaag
    54061 aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg agaccaagat
    54121 ttgattttga taaaaaacag tgggttgagt tagctactga agaagagaag agtggcacag
    54181 cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta ctgacaaaac
    54241 aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct caattgacga
    54301 tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc gggctttgaa
    54361 aatataaaac aattttatga ttggggatgt tatacggacc aagatttact tgactatgta
    54421 aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga aatttaatta
    54481 agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat aaaggagaaa
    54541 tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat aggcgggaca
    54601 attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt tcttgtgggt
    54661 gtggacttct taactggact tgctaaggca tggaaattga aagaaataag tagtgagata
    54721 ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt tgcggttgac
    54781 gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat gttctatgtt
    54841 gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt gccagataag
    54901 ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg aggaggaacc
    54961 aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg ccaataaacg
    55021 cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt agttctcaag
    55081 tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg acaggacgaa
    55141 atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat attgttggcg
    55201 atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc gctggttcat
    55261 atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat aaagtaacgt
    55321 ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat aaatacggta
    55381 ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat ttatgggtaa
    55441 ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa atgggtgtaa
    55501 gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac ccaacgactt
    55561 ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca actttatcag
    55621 atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg aacttacacg
    55681 tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg gactacaata
    55741 ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat gtaaatcaag
    55801 cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc aatttcccta
    55861 ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac actaaaggcg
    55921 gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga taaaaatagc
    55981 tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa aaatatttac
    56041 aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg acagcagaaa
    56101 ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct acgattggag
    56161 atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg cattctgtag
    56221 gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata atcattactc
    56281 aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac gcggataatt
    56341 ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag caagaacaaa
    56401 tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa gtagatatta
    56461 actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag ttattacaaa
    56521 caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat attgaaaagg
    56581 cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt atttctacaa
    56641 tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat gaaattataa
    56701 ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat ggaagaatgg
    56761 atattttccc taaactatat tcttataata tgtttggatt tgttatgaat attctgaaat
    56821 actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga tctactatat
    56881 taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat ccacaaatta
    56941 ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat acctatatta
    57001 atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg ttactaaaca
    57061 tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta ttaaatttaa
    57121 ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca attgatgttt
    57181 tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat tttgatattg
    57241 ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat aattaaacct
    57301 caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa taaaatgagt
    57361 ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa taataacaat
    57421 agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt ttgcagtcat
    57481 aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga aaagttaaag
    57541 taaataatag cataaaataa tatattttac aaagaataag tacaatctag ttttttgcta
    57601 ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca ttattatcta
    57661 gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttccgtaccc ttagctcagt
    57721 tggtcagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac agggtacatt
    57781 aacgtagcca tttgaatcgt tctgtgttag aattttttga agagtattat acaagctaaa
    57841 gcttttcttc attgccactc aaatgagtgg cttttttatg tatcctttta tggattaatg
    57901 aaaggatgtt tcacatagtt atatttctgt atatttgaaa agttttactt tgatttttaa
    57961 atagaaagac atttgggtta aattgtgaga taataataaa gaagagttta aagcgcacct
    58021 caaaccactt ccccataagt gtgttacgct ttaaactctt ttatatttga agctattaaa
    58081 aggcatacca tatttttgaa aaaaagtgag aaaaaaggct tacaattgga gtg
//
LOCUS       DAPBCS010000010        57516 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPBCS010000010 DAPBCS010000000
VERSION     DAPBCS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 57516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.010"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..2424)
                     /locus_tag="RD521_000791"
     CDS             complement(61..2424)
                     /locus_tag="RD521_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDT7770544.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="RD521_000792"
     CDS             2872..3093
                     /locus_tag="RD521_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7770545.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="RD521_000793"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="RD521_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDT7770546.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="RD521_000794"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="RD521_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDT7770547.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="RD521_000795"
     CDS             complement(5894..6205)
                     /locus_tag="RD521_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDT7770548.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="RD521_000796"
     CDS             complement(6202..6495)
                     /locus_tag="RD521_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDT7770549.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="RD521_000797"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="RD521_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDT7770550.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="RD521_000798"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="RD521_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDT7770551.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="RD521_000799"
     CDS             complement(8333..12685)
                     /locus_tag="RD521_000799"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDT7770552.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="RD521_000800"
     CDS             complement(12897..14606)
                     /locus_tag="RD521_000800"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDT7770553.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="RD521_000801"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="RD521_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDT7770554.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="RD521_000802"
     CDS             complement(16103..16903)
                     /locus_tag="RD521_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDT7770555.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="RD521_000803"
     CDS             complement(16900..17712)
                     /locus_tag="RD521_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDT7770556.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="RD521_000804"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="RD521_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDT7770557.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="RD521_000805"
     CDS             complement(18467..19189)
                     /locus_tag="RD521_000805"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDT7770558.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="RD521_000806"
     CDS             complement(19325..20206)
                     /locus_tag="RD521_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDT7770559.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="RD521_000807"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="RD521_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDT7770560.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="RD521_000808"
     CDS             21446..21925
                     /locus_tag="RD521_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDT7770561.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="RD521_000809"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="RD521_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /locus_tag="RD521_000810"
     CDS             23226..24011
                     /locus_tag="RD521_000810"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDT7770562.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="RD521_000811"
     CDS             complement(24357..26048)
                     /locus_tag="RD521_000811"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDT7770563.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="RD521_000812"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="RD521_000812"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDT7770564.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="RD521_000813"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="RD521_000813"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDT7770565.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="RD521_000814"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="RD521_000814"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDT7770566.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="RD521_000815"
     CDS             30332..31033
                     /locus_tag="RD521_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7770567.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="RD521_000816"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="RD521_000816"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDT7770568.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="RD521_000817"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="RD521_000817"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDT7770569.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="RD521_000818"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="RD521_000818"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDT7770570.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34337)
                     /gene="tsaB"
                     /locus_tag="RD521_000819"
     CDS             complement(33615..34337)
                     /gene="tsaB"
                     /locus_tag="RD521_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDT7770571.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(34528..35508)
                     /locus_tag="RD521_000820"
     CDS             complement(34528..35508)
                     /locus_tag="RD521_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7770572.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(35532..37016)
                     /gene="galT"
                     /locus_tag="RD521_000821"
     CDS             complement(35532..37016)
                     /gene="galT"
                     /locus_tag="RD521_000821"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDT7770573.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(37028..38017)
                     /gene="galE"
                     /locus_tag="RD521_000822"
     CDS             complement(37028..38017)
                     /gene="galE"
                     /locus_tag="RD521_000822"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDT7770574.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            38266..38433
                     /locus_tag="RD521_000823"
     CDS             38266..38433
                     /locus_tag="RD521_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDT7770575.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(38495..40306)
                     /locus_tag="RD521_000824"
     CDS             complement(38495..40306)
                     /locus_tag="RD521_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDT7770576.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(40303..40668)
                     /locus_tag="RD521_000825"
     CDS             complement(40303..40668)
                     /locus_tag="RD521_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDT7770577.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(40831..41226)
                     /locus_tag="RD521_000826"
     CDS             complement(40831..41226)
                     /locus_tag="RD521_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7770578.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(41244..42206)
                     /locus_tag="RD521_000827"
     CDS             complement(41244..42206)
                     /locus_tag="RD521_000827"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDT7770579.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(42206..42418)
                     /locus_tag="RD521_000828"
     CDS             complement(42206..42418)
                     /locus_tag="RD521_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDT7770580.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(42439..43137)
                     /locus_tag="RD521_000829"
     CDS             complement(42439..43137)
                     /locus_tag="RD521_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDT7770581.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(43157..43699)
                     /locus_tag="RD521_000830"
     CDS             complement(43157..43699)
                     /locus_tag="RD521_000830"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDT7770582.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(43831..44835)
                     /locus_tag="RD521_000831"
     CDS             complement(43831..44835)
                     /locus_tag="RD521_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDT7770583.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(44832..45821)
                     /locus_tag="RD521_000832"
     CDS             complement(44832..45821)
                     /locus_tag="RD521_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDT7770584.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(45818..46624)
                     /locus_tag="RD521_000833"
     CDS             complement(45818..46624)
                     /locus_tag="RD521_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770585.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            46790..47746
                     /locus_tag="RD521_000834"
     CDS             46790..47746
                     /locus_tag="RD521_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7770586.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(47823..48341)
                     /locus_tag="RD521_000835"
     CDS             complement(47823..48341)
                     /locus_tag="RD521_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7770587.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="RD521_000836"
     CDS             complement(48429..48578)
                     /gene="rpmG"
                     /locus_tag="RD521_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDT7770588.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            48806..49252
                     /locus_tag="RD521_000837"
     CDS             48806..49252
                     /locus_tag="RD521_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDT7770589.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="RD521_000838"
     CDS             complement(49447..51342)
                     /gene="asnB"
                     /locus_tag="RD521_000838"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDT7770590.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(51667..52641)
                     /gene="bsh"
                     /locus_tag="RD521_000839"
     CDS             complement(51667..52641)
                     /gene="bsh"
                     /locus_tag="RD521_000839"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDT7770591.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(52977..53064)
                     /locus_tag="RD521_000840"
     tRNA            complement(52977..53064)
                     /locus_tag="RD521_000840"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53030..53032),aa:Ser,seq:cga)
     gene            complement(53209..53775)
                     /locus_tag="RD521_000841"
     CDS             complement(53209..53775)
                     /locus_tag="RD521_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7770592.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            54030..55361
                     /locus_tag="RD521_000842"
     CDS             54030..55361
                     /locus_tag="RD521_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDT7770593.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            55327..55677
                     /locus_tag="RD521_000843"
     CDS             55327..55677
                     /locus_tag="RD521_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770594.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(55769..56188)
                     /locus_tag="RD521_000844"
     CDS             complement(55769..56188)
                     /locus_tag="RD521_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDT7770595.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(56189..56533)
                     /locus_tag="RD521_000845"
     CDS             complement(56189..56533)
                     /locus_tag="RD521_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDT7770596.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            56883..57479
                     /locus_tag="RD521_000846"
     CDS             56883..57479
                     /locus_tag="RD521_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDT7770597.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        16181 a        12116 c        10051 g        19168 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcaga ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatctttta tttcgttgat tctcgaataa tcaactctgt
    34561 ttccacaata atctttcttg aaaccggtgc tttggaatta cttaattgaa gaatcgtctc
    34621 cactgctagc tctcccatcc attcggtata aacttttaca gtagaaagag agggcgttac
    34681 atatttcgct acactgacat cattgaaacc catcacagaa atatcttctg gtaccactaa
    34741 acctttttct tgaatcgctt tcagtgcgcc aatcgctaat gcatcattag ctgcaaaaag
    34801 cgcatcagga acatgcggat tcgtttttaa atactgcttc actgcttgat atcctgactc
    34861 gacagaaaaa tctgcttcga taattggacc ttcgtcgtag cctttttctg ccaaaacttg
    34921 ttgaaatgcc ataattctgg gatcttttag ccgctgatgg tttttcttcg tatattctgt
    34981 tccagcaagc atagcgatat gttgcgcttt atgggagaca aaatgttcaa tgactttttg
    35041 aacacttgac ttaaagtcga caactagaga gtcgattcct tgagaagtgc cgtcagaatc
    35101 taccaaaagc aaggcatttt gggatccttt catttctaat aactgagctt cgtcgaattt
    35161 tcctagtgcg atcgttccat ctgtcggaag atcactaatg gaatcccacg tttctttcaa
    35221 taaagtaacg tttgcttctt ctgccttttt ttcgattcct agacgaatag ataaataata
    35281 aagatcttca agctcttccg tatcgtcgta ccactggatc aggcgaagcg tttgcttagt
    35341 atttgctgca tttttacgat gtttcgtata atttaagcgt tctgctgctt caaaaatttt
    35401 ttgctttgtg gctaagccaa cggaaagatc aggatcatag ttgagaactc ttgaaacagt
    35461 agcaggagaa actcctgcca actgcgcaat gtctttaatt gttgccattt gtctctcctc
    35521 cttttttacg tttataaaat tgcagtaaat cgttcaaacg ctgcctgtcc tttttcatct
    35581 cgcttgaata ctccagcgtc ttccagtaca cgtgcaaaaa cctgacctac agatttttgt
    35641 acgacatcgg tgaccgtatc tttcgtcaaa tcaggatgtt ctttttttaa ttgttctgcc
    35701 cacggacgat gatattcagc catctggttt tcttctccta ataagaaatg ctctacttct
    35761 actagttctg gtttcaaacg aggtggtaaa acagcaagcc ccattacttc gatcaaccca
    35821 atgttctcct ttttgatatg ttggacatct ttatgtggat gaaaaatccc atcaggatgt
    35881 tcttctgaaa cattgttgtc tcgaaggaca aggtccaact caaagtaatt gtcccttctt
    35941 cgtgcaattg gcgtaatcgt atggtgtggt gtaccatctg ctgaaaaagc tctgatagat
    36001 actgtttcat ccgaatatgc tttccacttt tccaatactg agttagctgc ttcaatcaac
    36061 aattttttct ctgttgattg caaacggatc acagacatcg gccatttcac gactcctgcg
    36121 atgacttgcg gataatctgc tagtttgata agtttttcta tcggtgcttt tgccatagga
    36181 aattcatgac gtcctgcctg ataatgatca tgagaaagaa tcgatcctcc aacaatcggc
    36241 aagtcagcat tcgaaccaac aaagtaatga ggcaagactt cagtaatccg aagcagtcta
    36301 gcaaatgttt cctttgtgat tttcatcggg cggtgttgtt ctgataaaat gatcgaatgt
    36361 tcattgtaat aagcataagg agaatattgg aatccccagc tttcaccgtc tagattcatt
    36421 cgaataatcc ggtgattggt gcgggctgga tagttcaagc gacctctata tccttcgttt
    36481 tccatgcaaa gcatgcaggt aggatagtct acttttaccg cctcgcgttc tgctgcgatt
    36541 tgttttggat ctttttctgg cttagataaa ttgattgtga tttccagttc accatactcc
    36601 gactctgccg gaaatttgat gttttttgcg atggctcttg ttttgatgta gtcatttttc
    36661 ttacttaaat taaagaaata ctcagttgct tcctctgggc ttttcgaata gtgctgtgca
    36721 aagaatgcat tgacgactga aggtggcggt gtgagaaaat ccattaattg cgcttcaaaa
    36781 atctcttgtt ctgctggcaa ttctgaaaga acaccatttt ctcttgcctt agcgacaagc
    36841 tgatccaata agtctacaga ttcttcctta actggtctta cttcaatgct ttctagagca
    36901 tcttctccta ccatccctaa aagacgattc tgtaaatata agcggtccat ttccatccat
    36961 ccgccagctt cgatcgcaag tgtggcaaaa tcacaaatcg tttgactgat tgacatatct
    37021 cattccccta ttcttcataa ccatttggat ggctgacatg ccaatcccat gctgttttga
    37081 taattgcttt gatatctgtg tattcaggtt tccaacccag aacctctctc gctttttcac
    37141 ttgaagcgac taatcggcta ggatctccag ctcttcttgg tgctatttct gctggaattt
    37201 cttttcctgt cacttctcta gctgcttcta gcatttcctt gactgagtat cctttgctgc
    37261 tgcctaaatt gaagaagttg ctttcatttc cttctttcaa gtattccaaa gctaaaatat
    37321 gtgcagcaat caaatcttct acttgaacat aatctctgat acatgttcca tctggtgtat
    37381 cataatcgtc tccataaaca gccaatgctt ttctttgacc aagagctact tgtaaaatga
    37441 ttgggacaag atgcgtttct ggtgtatgat cttctccgat agatgcatct gcttttgctc
    37501 cggctacatt gaagtagcgc aaagcgacat agcgcatgcc atatgcttgg tcacaccact
    37561 tcatcatttt ttccatcatc aatttacttt caccatacgg atttttagga ttagctggtg
    37621 tatcttctgt gattggagac tcttttggtt ctccgtaagt tgctgcagta gaagagaaca
    37681 cgattttatt cacattgaac tcgtgcatga cttcaagtaa gatttgcata ccgtatacgt
    37741 tgttgttgaa atacatcaat ggtttttcga cagattctcc tactaaagag cttgcagcaa
    37801 aatggattac tccttcaatc ggttcctttt caaatacgga acgtagaaat tctttatccc
    37861 ggatgtctcc ttcataaaaa tgagcatctg gatgtactgc ttgtttgtga cctgtgagca
    37921 aattatctac cacagctact tgatacccct tttgaactag ctggtctaca gcgtgcgaac
    37981 cgatataacc ggctccgcct agtactaaaa ttgtcataaa aaacactcct ttgattagta
    38041 aaattgtgtt tgttgattat atataattta aaaaaacgaa gtttactttt atggtgaaaa
    38101 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
    38161 attttcaagg attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
    38221 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
    38281 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggtcgcttct
    38341 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
    38401 aaaaaagcaa tgcgcaaacg cgtttctcgt taataattat ttaaaaacac gcatgaagac
    38461 ttgtgaaaga acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
    38521 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
    38581 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
    38641 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
    38701 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
    38761 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
    38821 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
    38881 caaatggcgg ttcgaacaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
    38941 tccgaatgac tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
    39001 gtatttcttt gtattccttt gcagaaaaaa cgtgccattc aatagcctgc cggattggat
    39061 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
    39121 ccgcttcttt tatctgccct tcaataaatg ctcggctagc ttctgcaaag gactcatcct
    39181 gataggtaag aggaatttct aaatacgacc aatcccatct ataagggatt agtgctggat
    39241 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
    39301 gaatcggcaa atgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
    39361 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
    39421 tttcttttgt tagtaaccgt ggccatgcca ttgatccgcc ttgcttttgg tttcttcctc
    39481 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
    39541 gatgatagac aagttttcct ttttcgttct cgaagcgccc ttctcttgat actgtaaaca
    39601 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
    39661 gtatcttcga aagatcgaca tctgcaggat tagaaggaag ctgatacagt aaatcatttt
    39721 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
    39781 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
    39841 cttttctttt gctgactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
    39901 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
    39961 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
    40021 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
    40081 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
    40141 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
    40201 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    40261 ctatacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    40321 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    40381 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    40441 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    40501 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    40561 taactgcgct ttttcttttg aaattcctcg tggccagaac ctatccacga gtattctata
    40621 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    40681 ctttctgcct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    40741 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    40801 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    40861 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    40921 atttgctgta cggatgctca agcttttctt ttgatcgtaa atatagacag gctggtcttt
    40981 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    41041 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    41101 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgc ttggccatta ctcgtagata
    41161 gacttcccaa aagatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    41221 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    41281 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    41341 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    41401 actcctccac ctaacacaat acttgacgga ttcaatgtat tccccaagtt ggcgcaagct
    41461 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    41521 gccatttcaa agatatcttt acttgttact tcttcaccgt tgtctaacat tgctttcagt
    41581 tttgaatctc ctgcgtattc ttctgccaag taacgaccta gacgtaccac accagtagcg
    41641 ctagacacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    41701 acagtgatat ggccgatctc tccgcctgct ccgctcacgc cgtgaatcaa atttccgcca
    41761 gcaacgatac caccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    41821 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcatcatt atcgatagcg
    41881 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    41941 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    42001 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg gttgattgat
    42061 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    42121 ttttgttgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    42181 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    42241 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    42301 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    42361 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    42421 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    42481 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    42541 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    42601 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    42661 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    42721 taagataaac aacgcaccga acaaaccgaa tatcgaagta ctcgcaccac cagacagaac
    42781 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    42841 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    42901 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    42961 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    43021 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    43081 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    43141 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    43201 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    43261 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    43321 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    43381 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    43441 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    43501 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    43561 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    43621 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    43681 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    43741 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    43801 aaatgattgg ccttcctttt ttaataaatt tcaactgttc agcgcgtgtt tttgtctgat
    43861 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    43921 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    43981 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    44041 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    44101 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    44161 ttgcgcgcgt tcttgaccaa atgatagcaa tccaatcctc tgtgcataaa gtcctcctag
    44221 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    44281 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    44341 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    44401 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    44461 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    44521 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    44581 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    44641 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    44701 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    44761 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    44821 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    44881 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    44941 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    45001 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    45061 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    45121 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    45181 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    45241 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    45301 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    45361 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    45421 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    45481 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    45541 cctagtaagc ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc
    45601 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    45661 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    45721 tttcccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    45781 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    45841 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    45901 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    45961 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    46021 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    46081 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    46141 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    46201 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    46261 actcgattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    46321 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    46381 ataatgtaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    46441 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    46501 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    46561 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    46621 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    46681 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    46741 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    46801 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    46861 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    46921 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    46981 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    47041 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    47101 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    47161 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    47221 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    47281 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    47341 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    47401 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    47461 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    47521 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    47581 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt
    47641 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    47701 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    47761 agaatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    47821 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    47881 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    47941 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    48001 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    48061 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    48121 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    48181 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    48241 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    48301 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    48361 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    48421 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    48481 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    48541 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    48601 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    48661 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    48721 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    48781 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    48841 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    48901 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    48961 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    49021 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    49081 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    49141 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    49201 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    49261 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    49321 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    49381 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    49441 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    49501 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    49561 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    49621 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    49681 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    49741 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    49801 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    49861 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    49921 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    49981 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    50041 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    50101 tgattcatat aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa
    50161 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    50221 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    50281 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    50341 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    50401 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    50461 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    50521 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    50581 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    50641 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    50701 gtctagtttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    50761 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    50821 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    50881 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    50941 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    51001 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    51061 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    51121 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    51181 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    51241 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    51301 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    51361 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    51421 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    51481 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    51541 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaacagcaaa aaaagacacg
    51601 acctaaagat cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt
    51661 cattaattaa ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc
    51721 atgacgatcc aagtcttctt tatttatatt tacagcagta atttgactgt cttcatacgt
    51781 acgataataa taaatccctt tatcaacgtt acagcaagaa gaataaatcg tatattcgta
    51841 ttttccttct ccagtatcgc atactccttt ttgttgctcg actgagccta gtatatgaaa
    51901 aaattgactg atactttcag attctgaatc acctgatgca gcattcattt tagtaaatgt
    51961 ggcttttaca aatcgagaaa cagaagagag atctccaggt aagcccaatc ctcccatccc
    52021 acgactataa acattcaagc tgatctggtt tgaaaaatga ttttctggtg ttccatttga
    52081 tagcgagcga taattgttca gattaaataa ttgataatca aatggcggat tatttgtcaa
    52141 cacaccgaca ggattatcat ataaatgaag tccatctttc atactttcaa ttactatcga
    52201 tttttcttta tcagctaata accagtgtaa aggtgacaag ggtagttttt cactaaaatc
    52261 gatattgact agattgatag tagccaataa ttctttagct tcgtctatgg ttgagcattg
    52321 ccctagtatc catggaataa actcaaatgg tgcaacgtta tcttttcctt cttgtaattc
    52381 tttataatca gcattgccag gaaaattcag tcctgccata ctcaaccctt tttcatttgt
    52441 agcctcataa tagagtggat agttggatac cccagctgct attcctatca ttgcataatg
    52501 tttatctaaa ttttttacct ttctaaaatc gaaacgataa tttcttggag taatagtgac
    52561 tacttcatta taagacatct cataatcaaa attccttcca aaataatggt ctttcgttgc
    52621 ataagtgatt gcggtacaca tcgttttccc tcctaaaaca aatcgttttt acacttttat
    52681 cttacatcta gtattttttt ttatcaaaaa aaaggccaaa gaagattgat ttactagcat
    52741 ttataaaaaa tagtactcag ggggaatcaa aaaatattgt aggttaaaag tacaacaata
    52801 aggggctgtg acaaaagtct tgtcacagcc ccttattccg aacaaacggt gttcaaaagc
    52861 ttgcatctgc ggtaataagc ccaaccaaac gaaaaaatag gaaaagctat ttttgtttgg
    52921 ccgttcttat tacttgatgc aggacagctt cttcacaacc tctaatcgtt gatataagga
    52981 aggtacagga tttgaacctg cgcgccggta ttagccggtt cgccggattt cgagtccggt
    53041 gcattaccac tctgccaacc ttccgtaagt actattttta aacttttttc acgaaatgtc
    53101 aagcctattt ctaatagata aataacagaa atcgcttttt ttagcaaaaa agtccattga
    53161 cagtttgcag ccaacggact taccgatcaa attcatactg atagaacact atctgattat
    53221 ttcaggatta tctaatgcta aaatcgagtc aattgtccgt ttgatcagtg caatgatttg
    53281 ttcttctgtt ggatgcatga tgtttgatga catcaatgac gctaagccag tcactgctac
    53341 ccatgtacca gtatgcagag catcgacata ttcatccgat aattgattgt atctttcatc
    53401 ttctttaact atttcacgga aataattgta tgaaaaatca tgcattttct ttccgtcccc
    53461 atgttcatca atgtacaatg ccatgtaaag actatgatgc ttttttgcaa agtagatata
    53521 gttcaaaccg agatcaatga ttttatcacc agtacgttca atagggaaaa catcttcttt
    53581 tagttgttca aagatagaat ccagcaacgt attttttaaa tcttccatgt ttttgaattc
    53641 cagataaatc ggctgtgtcg aaatccccat ctttttagca atatttctcg cagtaaatcc
    53701 tttaaaccct tcctttgcaa caacatcata agcagctttt agaatttgat ccttggtata
    53761 gacttttctt ctcattaaaa ctcccccact tctttactgc agtatatgtt gttttattgt
    53821 accctatttt tagcattttt tgcaaacatt ttactcaaaa cgttatattt taaatttttt
    53881 tcagtcttct ttaacttttt tttgcaaaat taaaaaaatt tggttaaatt caatgggttc
    53941 ccttacattt ttagttttga cctaaatgat agttgtccaa atctttcatt tcaagttata
    54001 gttaaagaaa atgaaaaaga aggtgtgtaa tggaaaacga aactgacgtt atttatattc
    54061 atcctcaaaa acgaatcgtt tcacagaaaa gaaagtattt ttatcttggt tttacaggtg
    54121 ttttttttct ttttattggc ttgctttcta atactcctac tgataattgg agtggattgc
    54181 taactattct tacctcgcca agcaatcttt tgacagatta ttttgcttta ggtgttttcg
    54241 gaagcgcatt cataaatgta gggattttga ccttactttc tgtgctttta gcttatcggc
    54301 acaaggtgat tttaaacggt cccttatttg cttcgattct aacagttacc ggtttctctt
    54361 tttttggcaa aaacctttat aattctatct caatcatttt aggtgtgtat ctatacgctg
    54421 tatttgtcaa taaacctttt tctcagtaca tcatgatcgg actgtttggt agcgtattat
    54481 caccggtcgt tagttatatc acttttggta tgcgttttcc tttactgata ggtattttat
    54541 tggggaattt agctggcata gcaattggta tacttcttcc gcccttagct gctcaaactt
    54601 tagttttcca tcgtggattc acactttata atattggatt cacctctggg ttgattgcga
    54661 tgacttttac tgctgttttg aggttattca gctattcaat cgtcgaaaat acattggttt
    54721 tcaatgaata tcactttcct ctcatttgga ttatttttgg ctttttttcg ctgacagtcg
    54781 gaattggttt ctattataat tcctttcggc tttcggggat tcgagaaatt ttcgattcat
    54841 ctggaaaatt gacaactgat ttcattgcta attctggtat tggtgctact ttgatcaaca
    54901 tgggcttagt tggtctgatg ctgtcttctt atgttttgct ggttggcggt cagttaaatg
    54961 gacctgtcat cggtgctatt ttatctgcgg tgggtttcag tgcttttggt tgtcatttaa
    55021 aaaacagttt tccgatacta gtcggaatat ttattgcttc tttgtttgga acttttcatg
    55081 agatcaccag tactggcatg cttgttgcag ctgtgtttgg gacaggtctt gcgcccataa
    55141 gcggttttta cggcagtttt tacggtgtaa tagctggtgt gctacatatt gctcttgtcc
    55201 ataacgtcag cactttgcac ggaggactaa atctatataa cagtggtttt tcaactggtt
    55261 tcgttgcagg tatccttgta ccgatactag ataactttac cgctgtcaga aaggaaaaaa
    55321 agacacttgg aaaaagaatt atcaaaaaga atcatcgatg agcttcttac ttatttatat
    55381 tcccatgaaa taactgaaat acgtattgga gtagaatttg ctgaggaagg gcttttcata
    55441 gagatacagg gtacggcaga aactcagcca gaagatttag atgtacttgc tgatttgctg
    55501 aacaatcctc gggaccctag tatggaaatc tactatcaag aactattagg taatccccga
    55561 catgatcggg aagattatca tctgttaggt agtttgattg atgacgtgga aatcatgtat
    55621 gcccagccga ttatcaatat taagatcttt cgacgtgctt ctcattccta ttattaaccc
    55681 tattttctat ttataaaaga ctgaatcaaa aaagcagctc ctgaaagttc gattaaaatc
    55741 tactttcagg agctgttcaa gcttgacatt attgattttc caatccttca tacacttcat
    55801 ctgttacagg ttctaaaaac tctgaattac cagcagtgat cgccaaatgt tcaaaccaac
    55861 tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg cgttacgacg acatctccag
    55921 ccattaatcg tcttgctggc tttccttctt cctgatacca gccttctcct ccggtcacaa
    55981 gtaagatttg atagccgtct gtatggacat gccagtgatt gcgacaacct ggttcaaaag
    56041 ttacattacc tacaccaaca gaaacttcgg gatcattcac taacatattc agatagcttt
    56101 gcccggtaaa tacttgagcg taagcttcgt ttttttcacc tgctggaaaa atcacgccat
    56161 tgtgtatttc ttcgtgtttt gtcatcattt acgcctcttt cttatcttcg ctattttctt
    56221 ctaaatagat ttcttttgct aaactaaaag ctgaccaagc ttttggccat tctacataaa
    56281 aagctaaatg ggtaatgatt tctgctattt cttcactagt aatgccattt tgcttgccaa
    56341 tcttcagatg agctggcaat tgttcaaaat ttcctcctga aattaatgca gatatggtga
    56401 tgatactacg ctgatgagca gataattctt ctctggacca tacttgtcca aataaaacat
    56461 catcatttaa ttctgcaaat tttggtgcaa aatctcctaa ttgatctcgt cctgcggttt
    56521 gtttttctgc catttctctt attctccctt cacgactgtt aggtgattca actatggttc
    56581 taaggcaact ttattttcta ttgtttcttc gcatttcatt ttaaatgtag tctttcagcc
    56641 tgtccaatgc ttagtttatt cagtcgatat gctttttaag catagttgct aacgcttttt
    56701 ttattttaaa aaaaacaagt tattaaataa gtctttagtt ggatgttttt ttgagatcaa
    56761 tcttttatac ttatgatgaa cttacaaaat tgtttgtttt ctgtcatatc aaaaaatggc
    56821 gcagttagtt gcttttctag taaagtatag ttaacaaaga acctctaagg agttgatgaa
    56881 taatgaatat tgctgcctct cgaaaactga taaatttcat atccattatc ggattaggcc
    56941 tatcaatcgc gctgacgatt tattttatta atttaggtgt tttcaaagat ttgaatgcgt
    57001 taagaggatt agtaggtgat tccatcattt taggaccgat tatttttgtc ttcatccaaa
    57061 tcctgcaagt agttattcca atcattcctg gagggatcag tactgcagct ggtgtcctga
    57121 tatttggccc ttatgctgga tttatctata actatattgg aatctgcatt ggttcgatta
    57181 tcatttttct gctagggcgt cgttatggaa agccattcat actttccatg attagcgata
    57241 agacatataa caaatatatt ggctggctgg ataatcaaaa ccgttttgag aagctgtttg
    57301 ctctagctat ttttcttcca gttgctcctg acgatgcgtt atgtttgatg gctggattga
    57361 caaacatgtc agtgaaacga tatacactaa tcattttgat tgctaaacca ctgtcgattt
    57421 tcttatatag tatggccttg atttatggag gtcaatatct aagtggtctt cttggataat
    57481 atttattttg taaaattagt cattgattga caatgt
//
LOCUS       DAPBCS010000011        55364 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPBCS010000011 DAPBCS010000000
VERSION     DAPBCS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 55364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.011"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            357..1628
                     /gene="serS"
                     /locus_tag="RD521_000847"
     CDS             357..1628
                     /gene="serS"
                     /locus_tag="RD521_000847"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDT7770598.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(2177..3661)
                     /gene="guaB"
                     /locus_tag="RD521_000848"
     CDS             complement(2177..3661)
                     /gene="guaB"
                     /locus_tag="RD521_000848"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDT7770599.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(4166..4861)
                     /locus_tag="RD521_000849"
     CDS             complement(4166..4861)
                     /locus_tag="RD521_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDT7770600.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(4951..6051)
                     /gene="ychF"
                     /locus_tag="RD521_000850"
     CDS             complement(4951..6051)
                     /gene="ychF"
                     /locus_tag="RD521_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDT7770601.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(6060..6251)
                     /locus_tag="RD521_000851"
     CDS             complement(6060..6251)
                     /locus_tag="RD521_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDT7770602.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6272..7162)
                     /locus_tag="RD521_000852"
     CDS             complement(6272..7162)
                     /locus_tag="RD521_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDT7770603.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7149..7916)
                     /locus_tag="RD521_000853"
     CDS             complement(7149..7916)
                     /locus_tag="RD521_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7770604.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(7928..8641)
                     /gene="rsmG"
                     /locus_tag="RD521_000854"
     CDS             complement(7928..8641)
                     /gene="rsmG"
                     /locus_tag="RD521_000854"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDT7770605.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8873..10774)
                     /gene="mnmG"
                     /locus_tag="RD521_000855"
     CDS             complement(8873..10774)
                     /gene="mnmG"
                     /locus_tag="RD521_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDT7770606.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10791..12188)
                     /gene="mnmE"
                     /locus_tag="RD521_000856"
     CDS             complement(10791..12188)
                     /gene="mnmE"
                     /locus_tag="RD521_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDT7770607.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12487..12870
                     /locus_tag="RD521_000857"
     CDS             12487..12870
                     /locus_tag="RD521_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770608.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(13038..13796)
                     /locus_tag="RD521_000858"
     CDS             complement(13038..13796)
                     /locus_tag="RD521_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDT7770609.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13814..14632)
                     /locus_tag="RD521_000859"
     CDS             complement(13814..14632)
                     /locus_tag="RD521_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDT7770610.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14629..14985)
                     /gene="rnpA"
                     /locus_tag="RD521_000860"
     CDS             complement(14629..14985)
                     /gene="rnpA"
                     /locus_tag="RD521_000860"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDT7770611.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(15103..15237)
                     /gene="rpmH"
                     /locus_tag="RD521_000861"
     CDS             complement(15103..15237)
                     /gene="rpmH"
                     /locus_tag="RD521_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDT7770612.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15760..17094
                     /gene="dnaA"
                     /locus_tag="RD521_000862"
     CDS             15760..17094
                     /gene="dnaA"
                     /locus_tag="RD521_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDT7770613.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17292..18422
                     /gene="dnaN"
                     /locus_tag="RD521_000863"
     CDS             17292..18422
                     /gene="dnaN"
                     /locus_tag="RD521_000863"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDT7770614.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18651..18893
                     /gene="yaaA"
                     /locus_tag="RD521_000864"
     CDS             18651..18893
                     /gene="yaaA"
                     /locus_tag="RD521_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDT7770615.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18880..20004
                     /gene="recF"
                     /locus_tag="RD521_000865"
     CDS             18880..20004
                     /gene="recF"
                     /locus_tag="RD521_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDT7770616.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            20001..21947
                     /gene="gyrB"
                     /locus_tag="RD521_000866"
     CDS             20001..21947
                     /gene="gyrB"
                     /locus_tag="RD521_000866"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDT7770617.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            21967..24438
                     /gene="gyrA"
                     /locus_tag="RD521_000867"
     CDS             21967..24438
                     /gene="gyrA"
                     /locus_tag="RD521_000867"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDT7770618.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24674..24973
                     /locus_tag="RD521_000868"
     CDS             24674..24973
                     /locus_tag="RD521_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDT7770619.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            25023..25535
                     /gene="ssb"
                     /locus_tag="RD521_000869"
     CDS             25023..25535
                     /gene="ssb"
                     /locus_tag="RD521_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7770620.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25561..25797
                     /locus_tag="RD521_000870"
     CDS             25561..25797
                     /locus_tag="RD521_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDT7770621.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            25940..27913
                     /locus_tag="RD521_000871"
     CDS             25940..27913
                     /locus_tag="RD521_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDT7770622.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            27919..28371
                     /gene="rplI"
                     /locus_tag="RD521_000872"
     CDS             27919..28371
                     /gene="rplI"
                     /locus_tag="RD521_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDT7770623.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28645..30012
                     /gene="dnaB"
                     /locus_tag="RD521_000873"
     CDS             28645..30012
                     /gene="dnaB"
                     /locus_tag="RD521_000873"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDT7770624.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            30792..32084
                     /locus_tag="RD521_000874"
     CDS             30792..32084
                     /locus_tag="RD521_000874"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDT7770625.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            32871..33734
                     /locus_tag="RD521_000875"
     CDS             32871..33734
                     /locus_tag="RD521_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7770626.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(33863..35485)
                     /locus_tag="RD521_000876"
     CDS             complement(33863..35485)
                     /locus_tag="RD521_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7770627.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            35734..36255
                     /locus_tag="RD521_000877"
     CDS             35734..36255
                     /locus_tag="RD521_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDT7770628.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(36441..38171)
                     /gene="spxB"
                     /locus_tag="RD521_000878"
     CDS             complement(36441..38171)
                     /gene="spxB"
                     /locus_tag="RD521_000878"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDT7770629.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            38336..39154
                     /locus_tag="RD521_000879"
     CDS             38336..39154
                     /locus_tag="RD521_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDT7770630.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(39257..39454)
                     /locus_tag="RD521_000880"
     CDS             complement(39257..39454)
                     /locus_tag="RD521_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770631.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            39623..40201
                     /locus_tag="RD521_000881"
     CDS             39623..40201
                     /locus_tag="RD521_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDT7770632.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            40296..40433
                     /locus_tag="RD521_000882"
     CDS             40296..40433
                     /locus_tag="RD521_000882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770633.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(41246..41749)
                     /locus_tag="RD521_000883"
     CDS             complement(41246..41749)
                     /locus_tag="RD521_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7770634.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            42108..43133
                     /locus_tag="RD521_000884"
     CDS             42108..43133
                     /locus_tag="RD521_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7770635.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            43480..44358
                     /locus_tag="RD521_000885"
     CDS             43480..44358
                     /locus_tag="RD521_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDT7770636.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            44834..44950
                     /locus_tag="RD521_000886"
     CDS             44834..44950
                     /locus_tag="RD521_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDT7770637.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            45878..46216
                     /locus_tag="RD521_000887"
     CDS             45878..46216
                     /locus_tag="RD521_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770638.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            46310..46540
                     /locus_tag="RD521_000888"
     CDS             46310..46540
                     /locus_tag="RD521_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770639.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     regulatory      47014..47128
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47264..48364
                     /gene="ribD"
                     /locus_tag="RD521_000889"
     CDS             47264..48364
                     /gene="ribD"
                     /locus_tag="RD521_000889"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDT7770640.1"
                     /translation="MSDIEYMKIAIELAKKGAGYVNPNPMVGAVIVKNNRLIGQGYHE
                     VFGGLHAERNALKNCRESPVGATLYVTLEPCCHYGKTPPCTETIIKSGITRVVVGTLD
                     CNPIVSGKGVKQLEENNIQVDVGILEIECQQLIKVFRKYITKRIPYVFMKYAMTMDGK
                     IATYTNQSKWISGEKARKQVHQFRHKVTAIMVGVNTVIQDNPLLTCRLENGKNPIRIV
                     CDTDLRTPITSKIIQTANDIKTYIATSSIDESKIALYRKCGCEIIYTKKKGNHIDLMN
                     LMQCLGNMQIDSLLLEGGSALNWSALEQQIVDEVQTYIAPKIFGGSGKSPVSGQGVVF
                     PKDAIMLKPYAFSQVGNDYLIESEVIYPCLQE"
     gene            48349..48984
                     /gene="ribE"
                     /locus_tag="RD521_000890"
     CDS             48349..48984
                     /gene="ribE"
                     /locus_tag="RD521_000890"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDT7770641.1"
                     /translation="MFTGIIEEIGKVERIQKDTYNCKLSIKSSKILEDIHLGDSIAVN
                     GICLTVTCFTRQSFIVDVMNETWNRTALNLLKHGSLVNLERAMHMNGRLGGHIVTGHI
                     DGTGRISSVRRDKNAVWYQINTQREILNLIVEKGSVTIDGISLTVAKVSKADFSVSVI
                     PHTLEQTILKNKQVGDMVNLENDILGKYVKKLIGNSNETELSKELLCRYGF"
     gene            49004..50209
                     /locus_tag="RD521_000891"
     CDS             49004..50209
                     /locus_tag="RD521_000891"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDT7770642.1"
                     /translation="MKYKKIKEALQALQEGKLVLVMDDKERENEGDLICSAQFATTEN
                     VNFMATYAKGLICMPMSESLANRLMLSPMVENNTDNHETAFTVSIDYKDTTTGISAEE
                     RGLTARMCVVEDINPFDFRRPGHMFPLIAKNGGVLERNGHTEATIDLLRLAGLKECGL
                     CCEIMNENGKMMRTSDLIQFSQIHHIPTLTIKELQEYRKVYDMLVECVSVVEMPTKYG
                     DFKAHCYINKLNGEHHVALVMGELNSGNDVLCRVHSECLTGDVFGSLRCDCGQQLDRA
                     MKMIAENGSGVLLYMRQEGRGIGLVNKLKAYHLQDKGMDTLDANLALGFQGDLREYYI
                     GAQILRDLGIKSLHLLTNNPDKVYQLEDYGMQISSRVPIEIKANPYDSFYLKTKKDRM
                     GHILNMEEK"
     gene            50210..50677
                     /locus_tag="RD521_000892"
     CDS             50210..50677
                     /locus_tag="RD521_000892"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDT7770643.1"
                     /translation="MNTFEGNLVAENIKIGIVVARFNEFITSKLLAGALDNLKRENVD
                     EKDIEVAWVPGAFEIPMIASKMVKSKKYDAIICLGAVIRGSTSHYDYVCSEVSKGIAQ
                     ISLNTETPVMFGVLTTDTIEQAIERAGSKAGNKGSECAQGAIEMVNLIRALEV"
     gene            51296..52510
                     /locus_tag="RD521_000893"
     CDS             51296..52510
                     /locus_tag="RD521_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDT7770644.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRIAVACGISREYYNRIEKGKQP
                     LNNELKEIIEKQIERFNPREPLFLLIDYFRVRFPTTDALKIIRDVLQLKADYMLYEDF
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLGCMT
                     AGGKLKRLDLAINDKAGILDIPKLIEKYKAGECISYFRKQKDYGGTEKNGYDTPQNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEVEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWKMNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDKENHTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTVVPQENDGIF"
     gene            53229..53439
                     /locus_tag="RD521_000894"
                     /pseudo
     CDS             53229..53439
                     /locus_tag="RD521_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
     gene            53551..55164
                     /locus_tag="RD521_000895"
     CDS             53551..55164
                     /locus_tag="RD521_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7770645.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFERPSFQRMIEDIEVGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAYYGA
                     YAPLGYKKHPDIKGKLLVDEETKWIVEKIFSLAYQGYGSAKITKILREEKVPTSSWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAQIKGILKSEVYIGNSVHNRQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIEKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     ATNKANKTPYSYYACSFYGQFGKGYCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LHKIQKVGNAERIREKKKKASALKKAENRQNEIDCLFAKMYEDRACEKITERNFIMLS
                     GKYQKEQIELEQQITDLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEENERIQEIEIYYRFIGKVD"
     gene            55262..>55364
                     /locus_tag="RD521_000896"
     CDS             55262..>55364
                     /locus_tag="RD521_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDT7770646.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAII"
BASE COUNT        17508 a        10010 c        11025 g        16821 t
ORIGIN      
        1 cttccaatca agaaaaatga aaaattacct attgatacac aggaagttat tgacgaaagc
       61 tgaaatattc gctattctta ttacaattat tagaataaat gaaaaacaca gaacccagta
      121 cactttattc tttttcagag agctgatggc aggtgtaaat cagcaaagga taatttggaa
      181 gttccagttc gtttacgtgc cctttcgaaa taagaaaggt cggttcaaca gccgttatgt
      241 gttcaacaag tgcagctgac gctgaacttg ggtggtaccg cgaatttcag actgattcgt
      301 cccaagaata atgggatgga tcagtctttt tttattttca aaaaggagga aatcatatgt
      361 tagatgtaaa aatgatgcga caaaatattg aagaagtaca agaaaaattg atgactcggg
      421 gcgtaaaaaa agaaacatta gctgaattca tggaactaga tgaaaatcgt cgtaagctac
      481 tagtcaaaag tgaagaatta aaaaaatatc gaaatgatgt ttctgctgag atcgcccaat
      541 tgaagcgtgc aaaagaagat gcttctgcta aaatcgctga aatgaaagaa gtcggtggca
      601 acatcaaagc attggatact gaaattgctg aagtggatga aaaattgaaa caaattgcaa
      661 ctactttgcc aaaccttccc catgattctg tgccagttgg aacagatgaa gaggacaatg
      721 tagaaatccg tcgttggagc gaaccacgta catttgcttt tgaaccaaaa ccacactggg
      781 aaattgcaga aaatcttgat attttagatt ttgaacgtgg cgcaaaagta gccggtagcc
      841 gttttgtcta ttacaaagga ttaggtgctc gattagaacg tgctgtctac aacttcatgc
      901 ttgatcagca tatttacgaa cacggctata ctgaaatgat cactccttat atcgtcaaca
      961 gtaaagcgat gtttggtacg ggacaatttc caaaattcaa agaagatgtt ttccaattgg
     1021 agaatactga tctaacatta atcccaacag ctgaagtacc attaactaac tattataatg
     1081 atgaaatttt agatggtgca gatttaccta tttatttcac tgcactaagc cctgcttttc
     1141 gttctgaagc cggaagtgct ggtcgggata cacgaggact gattcgtctt caccaattca
     1201 ataaagtaga aatggtcaaa ttttcggatg tagagcattc ttacgaagaa ttggaaaaaa
     1261 tgacagctga tgcagaagat atcttgaaaa aattgcattt gccttatcgt gtgatggcct
     1321 tatcaactgg agacatgggc ttttctgcag ctaaaactta tgacttagaa gtatggatac
     1381 ctgcacaaga tacttatcgt gaaatcagtt cttgttctaa ctgtgaagat ttccaagcac
     1441 gccgcgcaat gatccgttat cgcgataatg atggaaaaat ccactatact catactctga
     1501 atggttcagg acttgcagtt ggccgtacag tagctgccat tttggaaaac taccaaaatg
     1561 aagatggttc tgtcacagtt ccagaagcgc tcgttcctta tatggggaat ctgaaagtta
     1621 ttaaataaag aggctgtgaa aaagccgctt agtttcgagt attaagaaaa attttgtgaa
     1681 aatggctttc cacatttttg cataaattgg acttaatacc gagaaactgg cttttgaaca
     1741 ccatttatcg agaggttgtg aaatcagcga attaacctga gtattagaga aaaaaatgga
     1801 aaaatagtcc ctcatatttt accgtttgtt gaactaatac cgagggtcag ctgattgaac
     1861 accgtttagt ggagaggtcg taagcctacc ccccagctcc tagttacaag gacaaattca
     1921 aaaaacagct cttcatattt tatgaatgtt gtacttgtga ttcggaggtg gcaagggaac
     1981 actgtttatt caaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat
     2041 tcacgaaaat tgcttttcaa atttttgtga atttcttctt atttccgaag gagttgcttc
     2101 tacttccgcc gtttatacgt gtttagagcg tgagacaaaa gtgttcttta cttttgtccc
     2161 acgctcttta atgtttttaa gattcaactg aatagtttgg tgcttctttt gtgatttgta
     2221 catcatgagg gtgtgattct tttaatccac tgccgctcat ttggataaat tgcgcttcat
     2281 cacgtaattg ctgtagattg gctgccccaa cataacccat tcctgaacgc aaaccgccga
     2341 tcatttggaa aataatatct gcaacacttc ctttataagc aacccgacct tcgattcctt
     2401 ctggtactaa tttatttgct tcattgacac taccttggaa gtaacggtct tttgaacctt
     2461 tttccatcgc gcctaatgag cccatacctc ggtatgtttt gaatcgacgt ccttggaaga
     2521 tttcgaattc acctggtgat tcatctgtcc ctgccaacat acttcctagc atcactgcat
     2581 gtccgccggc agctaatgct ttgacgatgt caccagaata tttgatccct ccgtctgcaa
     2641 taatcgcttt gccatattga cgagcaactg atgctgcatc ataaatagct gtgagctgag
     2701 gtacacctac accggctacc acacgtgtcg tacaaatcga tcctggtccg ataccaactt
     2761 ttacgacatc tacacctgca tcatataaag cttttgtcgc ttcagccgtc gcaacgtttc
     2821 ctgcaatcaa tgttgcatct gcaaaagttg aacggatttc ttgaattttt cggatcacac
     2881 ctgcactatg tccatgagct gtatcgatga tgattgcatc tgcccctgct tctaataaag
     2941 cattcgcacg ctcaaatgta tcgcttgtta caccaacggc tgctgctaca agcaatcgac
     3001 catgttcatc ttttgctgca tttggaaatt cgatcacttt ctcgatgtct ttgatcgtga
     3061 tcaatccgct taaacgacct tcgttatcta cgattggtaa tttttcgatt ttatgttttt
     3121 gcaaaatctt ttctgcatct tttagagagg tacctactgg ggcagtcact aagttgtctt
     3181 ttgtcatcac atcagcaatc ggcatgtgat aatctgttac aaaacgcatg tcgcggtttg
     3241 taattatccc taccaatttt ctgttttcaa gtgtttcaac gattggcaca ccactgatac
     3301 gatatttgct catcaattct tctgcgtctg ctacaagatg ctgtggcgtt aagaagaacg
     3361 gatcaatgat tacgccactt tcagaacgtt tgacttttct tacttcatcc gcttgctggc
     3421 tgatcgtcat gtttttatgg atcacaccta gtccgccttg tcttgccata gcaatggcca
     3481 tcttgctgtc ggtaacagta tccatacttg cactgatgat tggaatattc aacttaatat
     3541 ttttagctaa ttgtacgctc atatccactt cgtttggtaa cacgtgactt tctgctggta
     3601 tcaataagac atcatcaaaa gtaaatcctt ttttcgtgaa ctttgtttcc cagttggaca
     3661 tttttagtac cactcctcta atttagttta tctattaaaa taattgattt tttcatttaa
     3721 gtcaatgatc tacttggaat caagttagct ttctcataga ataccaatat aaagcgttta
     3781 catctgtgtg tattaaaaga gattgtaaaa aagctgtcct gcatcaagta ataagaacag
     3841 ccaatcaaaa acagcttctc atgtttttga attggttggg tttattaccg cagatgcaaa
     3901 cttttgaaca ccgtttagtg gagaggctgt gaaaaagctg cttagtttcg agtagtaaga
     3961 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     4021 tggcttttga acaccgttta tcgtaaaaag ggactgtgac aagacttttg tcacagtccc
     4081 ttactattat ttaactatgg ataaacaaaa tgaggttctt tactacccct agatatctag
     4141 caaaatagct gatctttttg ctagtttatc ttcttgggac ggataaactt ccaataatcc
     4201 cgtatttttt cttcaagtag taacggattg ctaaagcagt tgccccgata atgataacta
     4261 ctactggatc taatactgga ttgataactg gtggcaatag tgttgtcgca gaaaacagta
     4321 cgacccaaag aagtgtcgtt ccacctagga taagcaatgt cttacccatt cctggtttct
     4381 tgcttcgatc tgctccggga tattcatatt gatagatgta tttgtacatc atataaaaga
     4441 cccagccacc gattgcagaa cctagaacga gtgtagaaaa gccataagct ggcgttgttc
     4501 cacgtgaaaa caatccggta atcgcaatca tgatacttaa tagaccaagc aacagcaacg
     4561 tattatctaa ccacatcaat aaaggggtgg atgaattttt tacttctgct ggcttattga
     4621 tgattgcttc cgttctttca gagactgtcc caaacaattg acgggctgtt cttccgcctt
     4681 tttgttctgc aactaaagta ggcagaatgt catgaagggc ttttactttt tcttcttcag
     4741 acaaattcgc tgcttctaaa gattttttca aatcaaaaat gtactgttga tttcttttgg
     4801 ttaattttgt ttctaaacta ttgttttctt ttacgattgc tcgtaattgt tctggttcca
     4861 ttatctaata gtcctcctta gccgttcctt ttctcaacgg tcgtcacaaa agacattcta
     4921 ccatattcta cagctagttt gatagccctt ttacacatta aagcggaata acattacgtc
     4981 gccgtcttgt acgacataat cttttccttc taatcgaaca cgtccagctt cttttgctgc
     5041 atgcatgttt ccgtaatgat ccaagtcttc aaaagagaca gtttctgcac ggataaatcc
     5101 gcgttcgaaa tctgaatgga tgattcctgc tgcttgtggt gctttgattc cttttttgaa
     5161 tgtccaagca cgtacttctt gttccccagc agtaaagtaa gtagctaagc ctaacaaatc
     5221 ataagcagca cggatcaact gatccaaacc tgattcttcg attcctaaag cttctaaaaa
     5281 ttccgctttg tcttcttcat ctaattctgc gatttcttct tctgcacgag cactgaccac
     5341 gatcacttct gcattttctt ctgcagcaaa gttgcgcact tcttgtacat aatgattcgc
     5401 gtctgcatct gctacgtcat cttctgagac attggctaca taaagaattg gttttgtcgt
     5461 caataaaaat agtgatttaa cgattttttg ttcttcttca gtaaattcga tcgttcgggc
     5521 agattttcct tcttccagta caggtttgat tttatccagt acagctagtt ctgccactgc
     5581 atctttatct ttggttttag caacttttgc tacacgagta tagcgtttgt tgatagattc
     5641 taagtctgct aacactagtt ccaagttgat cgtatcaata tcttctagtg gatcaacacg
     5701 tccttctacg tgagtaatat tatcatcatc aaaacagcga acaacgtgac aaatagcgtc
     5761 tacttggcgg atatggctta agaattgatt tcccaaacct tctcctttac ttgcgccttt
     5821 tacgattcca gcaatatctg taaattcaaa agtcgtcgga acagtttttt tcggatgtac
     5881 aagttctgtt aagcgttgca aacgccagtc aggtacttct accatcccta cgttggggtc
     5941 gatcgtcgca aagggatagt ttgctgcttc tgctcctgct tttgtgatcg cattaaacaa
     6001 agttgatttt cctacgttgg gcaacccaac aattccagca gtcaatgcca taaatttatt
     6061 cactctttct ctgcattttt ttcaattact ttttttaatt tcttttcaaa atctcgacgg
     6121 gtcatcatga cgatatgtcc gcaattcata catctgattt tgatatctgc gcccattcga
     6181 atgacttccc agcgattcgt ctggcaagcg tgaggttttt tcatttcaac gatatcgtgc
     6241 aagtcgtaca taaaaatagc ctcctttttg tttattcttc atcaaattga atattcaaga
     6301 tatcaagaat tcgagtcaaa tctttttgag aaagatattc aatctcgatc ttacctttcc
     6361 catttttttc ttggatagct acacttgtac caaatttatc catcaaacga tcttcgcttt
     6421 ctcggatata atatggtttt tcctttgcta gccttgggat ttttttcttt tcttttccat
     6481 cattttgatt taactcagaa acaagctgtt ccagttggcg gacagttaaa ttttcagtta
     6541 ctgcacgatt ggctaatttc aagatcaatg ttttattttt taaacctaac agcgtacggg
     6601 cttgtcccat agataaacgc tgatcttgta ccatttcctt gacaagatcc ggtaaagaaa
     6661 gcaagcgcaa ataattggcg atataaggtc tgctctttcc taaacgacta gctacttctg
     6721 cttgtgttag tttcaggttt ttcatcagca tctcatatgc ttctgcttct tctaacggat
     6781 tcagatcttc tcgctgcaag ttttccagca ctgctacttg catcatcgct tcttcgtcaa
     6841 agtctctaac gatagcaggg atcgtttctt tttcagctag tttggacgct cgaaaacgtc
     6901 gctcccctgc aataatctca tatcctttta ctgcggaacg gcgtacgatg atgggttgaa
     6961 agacacctga ttgttcaata gaattagcta gttcctgcaa agatacttct tcaaatgttt
     7021 tacggggctg atacggattt ggtcgcagct cattcaatgg tagctggaca acggtatcat
     7081 cttttacatc caccgtctct aagttttcta aatcttgaaa cagtgcatcg attcctcggc
     7141 ctaatccttt acttttcttc acgacttacc acttcctttg ccaatgcttg gtagacttca
     7201 gctcctcgag aacgtggatc gtagtcgatg atcggttttc catgactagg tgcttctgat
     7261 aaacgaatat ttcttggaat gatcgtttcg taaacttttt cgcggaagta tttacgtact
     7321 tcttctacga cttcatttcc cagatttgtt cgtgcatcat acattgtcag taagactccc
     7381 tcgatctcta attctggatt aaagtgtttt tggactaaac gaaccgtatt cagtaattgg
     7441 cttaatcctt ccaatgcgta atactcacac tgaacaggta tcaaaatcga atcacttgct
     7501 gtaaatgaat tgattgtcag atggccaaga gaaggcgggc aatcgatcaa aatataatca
     7561 tactgactgc ttacttctgc taaagatcct tttaatcttg attctcttgc catcattgac
     7621 gtcaattcga tctctgctcc tgctaattgg agagtcgcag gcacaatact caaattttcg
     7681 tgttcagtga tcaaagttgc ctcatcaatt ggcaattcat tgactagaac atcatagatg
     7741 tctcttgtca catcaggttt tctgatcccg acaccactgg tagcatttcc ttgtgcatcc
     7801 atatccacta gcaatacttt tttgccaagg ctagcaagac aagcacctag attcaccgtc
     7861 gtcgttgttt tgccgacgcc acctttttgg ttcgcaacag atattatttg tgccatctct
     7921 tctcctccta tttgattggc tgcttattag gcagtccggg ctttcttggg tatttctttg
     7981 gggttggttt ctttttttga ataacgatga tatgccgttg atcagagaca ttcggcaagg
     8041 taaccgcctc ttctttgagg aatttaccac ctaataaagc aatcgcttgt tttgcttctg
     8101 tcaattcctc ttcacttttt gctgctttca atgcataaaa atagccttct tgtttagcca
     8161 agggcaaaca caattccgct aacacattca aacgagcaac cgcacgagca gtgacaaggt
     8221 caaatgatgc acgaaagtga ggattttgcc caaatgtttc tgcccggtca tgatataagg
     8281 aagcatcgat tcctaataat cgggttaact cagataagaa ggttattcgt ttattcagag
     8341 aatcaacgat cgttatctgc agttctggaa aggcaatttt taaaggtatg ctaggaaacc
     8401 ctgcccccga accaacatcg cataaagata aaggttgcgt aaagtctact tcaaatgcta
     8461 atgtaagtga atcgtaaaaa tgcttcagat atacgtcttt ttcatccgta atagctgtaa
     8521 ggttcatctt ttcgttccat tcgactaatg tccgataata aatagcgaat tgattcattt
     8581 gatgatcggt aagctctatc cctttttctg ccaaggattg gcgaaattct tctggtatca
     8641 taaatattcc tttcattgtg aaacagcaat tgtttcacag attctctccc tactttaacg
     8701 caaaatctct agacatgcaa tccttccata tggattctta aaaatttgaa aagtaagaaa
     8761 agccgataga ctttttcttt ctagtatgac tcataaaata tacacagaag agagaactag
     8821 ctttgagcaa tttctgcaaa aactagttct ctcttctatt tattattacg tattacgctg
     8881 gttcactgac tttcgcaatc ttcccttgtt cgatatagac cattaggata cttaggtctg
     8941 cagggtttac gccactgatt cggctagctt gggcaattgt ttctggttgg attttctgca
     9001 atttttgttt tgcttctgtt gctaatccgt tgattgctgc gtagtcgatg ttttctggaa
     9061 tctttttcgc ttccatgcgt tttaattttt ctactttttc catggctttt ttgatgtagc
     9121 cttcatattt gatttggatt tctacttgtt cgatttcttt tcgtgtcaat tcttcatttt
     9181 ctggaataaa ccgtagaagt tctgcataag taatctcagg acgtcttaaa aagtcgcttg
     9241 ctagtacacc atcttttaat ggtgccgcat taatcgttgc taaaaagtct tggacttctt
     9301 ctgttggttt gattcgtaca ttcatcaaac gtttgatttc ttgttcaact gcttcttttt
     9361 tcttcaaata attcttgtat tgatcttctt ttactaagcc tacacggtga cccatttctg
     9421 ttaatcgcaa atctgcatta tcatgacgta aaagcagacg gtattcagca cgtgaagtca
     9481 gcaaacgata aggttcgttc gttccttttg ttaccaaatc atcgatcatg acaccgatgt
     9541 aaccgtcact tcgtttgatc actaaaggtt cttttccctg aactttcaaa gctgcattga
     9601 taccggcaat cagcccttgt ccagccgctt cttcataacc gcttgtacca tttgtttgtc
     9661 cagctgtata aagattttca acgactttag tttctaatgt cggacggagc tggtgtggct
     9721 ctactacgtc atattcgata gcatatcctg tacgcatcat ttctactttt tctaatccag
     9781 caatagaatg aagcatgttg acttgaacat cctctggtaa agaagtagat aaaccttgaa
     9841 catagacttc ttccgtattt aagccttctg gttccaaaaa taattgatga cgtggtttgt
     9901 ctgcaaaacg cacgatctta tcttcgatgg atggacaata gcgagcgcct accccttcaa
     9961 cgatccctgt aaacattggg gcacgatgta agttgtctcg aataatttgg tgagtcgtct
    10021 cacctgtata ggtcagccag caagattcct gatctagttt ataggcacta tctggtgtgc
    10081 tgaagctaaa atgattcggc tctttgtcac caggctgctc ttctgtaaca gaatagtcga
    10141 tcgtacttga ttttactcga ggcggtgttc ctgttttgaa ccgttcgata gcaaagccaa
    10201 gttcttttag atgattcgct agtccaatag aaggttgaga gttattcgga cctgatgaat
    10261 atttcaattc accgataatg atttctccgc gaagtgctgt tccggctgta ataacaactg
    10321 ctttggcccg gtagctcgct cctgtggacg tcactactcc atgacaaaca ccatcttcga
    10381 cgatcagttc ttcgacgatt ccttgtctta gcgtcaaatt tggttctttt tcgatcgtat
    10441 gtttcatttc tgttgaataa gcatgtttgt ctgcttgtgc tcgcaatgct cggacagctg
    10501 ggccttttcc tgtgttcaac atacgcattt ggatataggt tttatcgata ttcttaccca
    10561 tttctccgcc taacgcatcg atttctcgaa cgacaacgcc tttagctggt ccgcctacag
    10621 aaggattaca tggcatgaat gctaccatgt ccaaatttat tgtcagtaat aatgtttttg
    10681 atcccatacg tgcagcagca agtgcagctt ctgaaccggc gtgtcctgcg ccaacaacga
    10741 tcacgtcata cgcatctgct tcataatgtt ccaatcgttt gttcctcctt ttattttcct
    10801 aaacaaaatt gactgaataa ttgagtgatt agctcatctt gtacactgtc gccgacaact
    10861 tctcctagat agtcccagca acgcgtcata tctatttgaa cgagatccac aggcattcct
    10921 gaatcaatac ctgcaatgac ttcttcaagt gagagggaag ctttttctaa caacgcgata
    10981 tggcgggtat tagaaacata agtagcgtct ctttctcctg tttctccgct gaagaataaa
    11041 tcagaaatag ctgattccaa acgatctagt ccgtcgtttt tagctacgga aattgaaaaa
    11101 actggttcat tttcgatcaa tttcttcaat ttttcttgtt ccagctgtgc tggcaaatct
    11161 gttttattta gaagaatgat ccgtttcaat ccacttgtcg cttccaataa ttgttcatct
    11221 tctgctgtca gtggttcgct ttggtttaga acaagtaaga tcaagtctgc ttctgctaat
    11281 gcttttcggc ttctttcgac cccgatccgt tccaccacat cttctgtttc ccgaattcca
    11341 gctgtatcga ttagcttgag cggtaccccc cgaacattga catactcttc gattacatct
    11401 cgagtcgttc ctgcaatatc cgtaacgatt gctttttctt ctcttaacaa atggtttaac
    11461 aaacttgatt tgccgacatt tggccggccg atgattgccg tacttaaccc ttcccgcaga
    11521 accttccctt gcttggatgt agcaagtagc gcttggattc tttgttggat catctgcgct
    11581 ttttcaagca ataacttcgt agtcaattct tcgacatcat catattctgg ataatcaata
    11641 tttacttcta cttgtgctaa tgtctctaaa atctcttgtc tcaatgagcg gatcaaagca
    11701 gacagattgc cgtctaactg attaagagct agtcccattg ctttgtctgt ttttgcccga
    11761 atcaaatcca tcacagcttc tgcctgtgaa aggtcaacac gaccattcaa gaatgctcgt
    11821 tttgtgaatt cgcctggctc agctaatctt gcaccttctc ttaacaatag ctgcaaaatt
    11881 tgattgacta caacgatccc accgtgacaa ttgatttcta ctacatcttc ccgcgtaaat
    11941 gtctttggtg ctctcatcac ggagaccatg acttcatcga caagctgatt gctcttggga
    12001 tcaacgatat gtccataatg gatcgtatga cttggtactt ctgataatcg tttgtttcca
    12061 gattgataaa ctttatctgc tagttgcaca gcttgatcgc cacttaaacg aacgatactg
    12121 attgcacctt ctccaggtgg ggtagaaatt gccgcaatcg tatcaaattc taaagtaatt
    12181 tcagccattt tttcacttcc tttttcattt tgcgcacaaa aaaagtgccc actccaccct
    12241 ttatgcacaa taaacggtga acttcgcact ttgactgctt gttcttgatt aaattctggt
    12301 catagattat cacacttgct ataaggaatc aagtgtttaa aacaattaga tgaagttgag
    12361 cctgttctgc ttccattacc ttatatttaa ttgtccttgg atgtctcttt gcgttctttg
    12421 gtttattcga agagaagata ctgtataaac aaggctttat ctttcttaaa aaaaacggta
    12481 cactaaatgg tagcgctatc taaactatac acttttgaaa ggagaaaata tgtgagcccg
    12541 ttcagatctg ctgcattgga agaagaaagt gaccctcgga tcaacgatgt cgttttatct
    12601 acagggaata tcaaccagaa atatatcgtc cgcgatatcg tttttgttgt ggaatgctta
    12661 gaagctgatt tgtttgaccc atcttttccc aaagatgacc ttttcttaac aacgaagagg
    12721 aaattaaaga cgaaggtact tgaatatggt gcgaatgcag taatcaactg tcattttgag
    12781 tatcagcaaa aaaaacgcga cggaaagtat tttctggaac tttttgctta tggaacagtg
    12841 atccaattta tccaaacaac gattggttaa atgaaacaga caaaaaaagc ggatcaaaac
    12901 tgaagacagt ttcgatccgc ttttttctat caaagttagt gactacttca tcgtatactc
    12961 tgataagaat gatttctagt aaaatatact tggtttgaaa aactttgtca acgtgattta
    13021 ttcaaaaagt aatggaatca ataatatttt ttagcaggtt ctactactaa ataacgataa
    13081 ggttcctcac cttccgaatg tgtttttaca tgttcatctt tgcttaatgc agcatggatt
    13141 tgtttacgtt caaatgcagg cattggttct aagaaaacag gctgccctgt ccgttttact
    13201 ttttcagccg tgcgatcagc taaacgctga atgatctctt ggcgcttttc acggtaattg
    13261 cctacattga ctactacaga cagcttattt cttgctaaac gatggatgaa gacttgtgcc
    13321 agatattgca atgcattcag tgtttttcca tgtttcccaa tcagtatgcc ttgcttttgt
    13381 gtttctaaat ggaaaacaac catattattt gattctcttt ccattttgac aagagctgga
    13441 gcatcaagct gttttgaaat ttcagtaaga taaactgcca attccgttaa agctgcttca
    13501 tcacctagct cacttacttc ttctgttttt tcttcaagaa tcgtttcgtt gactacctcg
    13561 atctcagctg ttggttcttc ttccaaaatt tcttctacag tctgttctac tgtttcagaa
    13621 actttttcgc tgatacttgg ctggatcgaa agccgtgcat tctttcgacc cattcctagg
    13681 aaaccttttt ttccttcttc cagtatttcg atttcgactt ggtctttggc caaacccaaa
    13741 tcagccagac ctagttggac agcttcgtct attgttgcag cttcataaat cggcattagc
    13801 attacctccc ctattatttc cgttttttct taggattcat tgctttctta agagcacgtt
    13861 ctctttcgcg gattctccgt gcttcttctt cacgctcttt tcgaattttg aaagggttgt
    13921 tgatcagcag cgtttgtaca acttggaagg cattggaaac tacccagtat aatgaaagac
    13981 cgctggctaa attgacaccc atcactaaaa tcattacagg catcacgaaa ttcatgatct
    14041 tcattgatgc attcgattca atttgactca tgctagataa atacgtactt gcaaaagtaa
    14101 agattgctgc taaaatcggc aaaataaatg ttggatccgc gttccctaag ttcaaccata
    14161 agaaatgccc ttgtttcaac tctggtacac gtgaaataga ttgccataac gccatcaaga
    14221 ttggcatttg tactaataaa ggaagacaac cagcatatgg attgacgcca ttttctgcat
    14281 ataaacgctg ctgctcttct cttaacttac tttgtgtttc cggatctctt gaactatatt
    14341 gttgctgcag tgcttttaat ttcggttgta attcttgcgt tttacgcatg ctctttgttt
    14401 ggaaatgcat caacggcaat aggatcacgc ggatgatcag tgtaaataaa atgatcccta
    14461 tccctgcatt accaaatgac aacgccttga tcgcctcagc aaagtaataa acaatataac
    14521 gatcccaaat tcctgtactc tgtgcgctga cttccccagt tccgcaaccg gataagacga
    14581 tgaccaaact aacgataccg gccatcaaaa gtaaacgttt gtatttcttc acttactctt
    14641 ttcctctctg tcgattattt ttgcaagttt taagacgtgg actaaattgg agcgaacctc
    14701 atcataagtc aaccccttca cacttggtct agcaatcaca ataaaatcca actccggatc
    14761 aatttcttct tttatagaat aaactgctgc tcggattttt cgtttgactt cattgcggtt
    14821 taccgcgttg ccgacctttt ttcctacaga taaaccaact cgaaaatgag gctgtcccgg
    14881 tttttccaaa cggtaaacga cgaatttgcg gttagcaaaa gaatctcccg tatggaacac
    14941 ggcttgaaac tctcgttctt tttttactcg atacgctttt ctcattagtt ttctttcctt
    15001 ttctatctct cacttacgct cataatcata ccataattac gattgtcctt tcatcatttc
    15061 cctaaaaaag agtataaaaa aaaccactga aaccccagtg gcctaagctg aaagaacttt
    15121 tcttcctttg cgacgacggc tagctaacac acgacgtcca tttttagtac tcatacgttt
    15181 acggaatccg tgaacttttt gacgtttacg tttatttggt tggtatgttc ttttcatata
    15241 ttccacctcc aaaatgttct tatctatttt ataaacacag acatactttg atagtataac
    15301 gacaaagtag tgattttgtc aatataaaat agaagatcga ttgttaaaac ttatccacaa
    15361 gattattttt ttctatggga atcctatgtg gaaaaagtta tgcacaggga ttatccacag
    15421 ataataaaag gctggtttta cacagaaaac acacgtgtaa aaaaagtaat ccacaatcag
    15481 gcgatttcct gtgtattccg tctcctaact attggtatat ctgtattttt tatccacaga
    15541 cgcaagagga aatctattgg tttttacgag ttttccacct gtgcattagt ggtgtggaaa
    15601 actttttttg acaactgtgt ttttctcttt tttctttttg tcgattgtgt ggataattgt
    15661 aattaaaaaa tttttttctt cggtttctgt ggaaaactat tgaaaaaaat gctaaattag
    15721 tactagttat tcacttttta atgaaggagg aggccttata tggtatccct cgatgcttta
    15781 tggaatgaat taaaagcaac ataccaaaag gatttatcgc ctgctagcta taatacatgg
    15841 atcgaaacag cgaaccctcg aacccttgat caaaaccagc tcgttgttga ggttcctagc
    15901 aagattcata aagaatattg ggaaaaaaac ctggcgacaa aaatcgttga agtcggttat
    15961 atgttatcag gaaatgagat tatccctcgc tttattactg gcgaagaagc agagcaagaa
    16021 gaagttatag aagaaaaaaa tccaaaagtt gtggcaccta gtccgctaaa aaaagccatg
    16081 ctgaacccta aatatacctt tgatacgttt gtcattggta aaggaaacca gatggctcat
    16141 gcagcagcgc tcgttgtagc agaagatcca ggttctattt ataacccgct cttcttttat
    16201 ggaggcgtgg gacttggtaa gactcacttg atgcatgcaa ttggtcatca aatgctgcaa
    16261 agtcagccta atgccaaagt aaaatatgta agcagtgaaa cttttgctaa tgatttcatc
    16321 aattcgatcc aaaacaaaac agcagaagag ttccgtcaag aatatcgaaa tgttgattta
    16381 ctgctagtgg atgacattca attttttgca gaaaaagaag ctacgcaaga agagtttttc
    16441 catactttca atgccttgta caatgaaggg aaacaaatcg tactaaccag tgatcggttg
    16501 ccaaacgaga ttccgaaact gcaagaacgc cttgtttctc gctttgcatg ggggctgtct
    16561 gttgatatta caccgccgga tcttgaaaca agaacagcga ttttacgtaa aaaagcggcg
    16621 gctgaacgtc tagagatccc agatgacaca ctaagctaca tcgcaggaca aattgattct
    16681 aacattcgtg aattagaagg tgcgcttgtt cgagtgcaag cttttgctgc gatgaatagt
    16741 gaagatattt cgactagtct agctgcagac gcactgaaaa cgttaaaatc aggaaaagga
    16801 catcctcaat tgtcaatcct gcagatccaa gaagaagtgg caaagtatta tcacatccaa
    16861 ttgaaagatc tcaaaggaaa gaaacgcgtc aaatctattg ttgttcctag acaaatcgct
    16921 atgtatttgg cacgtgagct cacagataat tctttaccga aaatcggagc tgaattcggt
    16981 ggcaaggatc atacaacggt tatccatgct catgaaaaaa tccagcagct gatggattct
    17041 agtgtcagta tgcaaaatga agtatcagaa atcaaaaact ttttactaaa ttaatttgtc
    17101 gattctatca actgaaaata gcatgtggat aaaagaaaaa aacgttcaaa agttatacac
    17161 actttttcca cagctgtttt ttcgtttctc tcttacgttt tttgagtttt ccacagaatc
    17221 aacatgccct attactttta ttattctttt taactaataa taaataaaca aacattcaaa
    17281 ggagatacat catgaaagtt actttaaacc gagctagctt tatgcaggaa ttgcaaactg
    17341 ttcaacgagc tatttcaagc aaaaccacga tccctatttt gacaggtgta aaaatcacac
    17401 tgacacaaga aggtttgact ttgacgggga gcaacgctga tatatcaatt gaaacttttt
    17461 tgtctgttga aaacgaaaaa gcaaatatgc aaatcgaatc tactggttcc attgttttac
    17521 aagcacgttt ctttagcgaa atcattcgga gacttcctga agaaacattt actttagaag
    17581 ttttagaaaa taaacaagta gcgatcactt ctggaaaagc gaattttatc gtaaatggat
    17641 tagatgcaga taactatcct catcttcctg ttgtcgaaag ccataaccag atgaaattac
    17701 ctgtacacgt attgactaaa ctaatcaacg aaacagtttt tgctgtctca caacatgaga
    17761 gtcgtccaat cttgacaggt gtgcatttca ttttatctga taattcttta ttagctgtag
    17821 ctactgattc tcaccgtcta agtcaacgcg tgattccagt agaacaagcg gctgatcatt
    17881 ttgatattgt tattcctgga aaaagtttga tcgaattatc tcgctcatta acaaatgaag
    17941 aagaaatcgt cgaaatcagc attatggaaa accaagtgtt gttcaaaaca gaaacgatgt
    18001 atttctattc ccgtttgcta gaaggaaact atccagatac caatcgtttg attccttcaa
    18061 gttttaatac agaggttgaa ttttctgttc ctagcttttt agcggcgatc gaacgggctt
    18121 ctttactttc tcatgaaggc cgtaacaaca tcgttcgttt atctattcgt ccagatgcgg
    18181 ttgttttata cggaaattcg ccagaaatcg ggaaagtaga agaaagcttg agttatacag
    18241 caagcagtgg cgatccatta gatatttctt tcaatccgga ttatatgaaa gcagcgttgc
    18301 gtgcatttgg agatatgagc atcaaagtga aatttatctc tgctattcgt ccatttacat
    18361 tggaaccaac ggaagatggc gttcaattta ttcagctgat tactcctgta cgtacaaatt
    18421 aacaatgatt ttcaatgttc ttgaaaattt aattgaaaag aaatagaaaa aataaaattt
    18481 taaacagaag gccctaaaat ctcttttaag gctttctgtt tatttttgtt taaatcacct
    18541 tttttagatt aaatcgtctt atatggaaaa atatatggat aaatttgttt tattgcgaaa
    18601 aaaaaggtat aatagagtat acttttaata atgaaaatga gggatgggca ttgaaacaac
    18661 aagtcattct aaatacggaa ttcatgacgt tgggccaaat gttgaaagaa gtcacagtta
    18721 tcggtagtgg cggacaagca aaatggtatt tggcagaaaa cactgtgtta gtcgatggcg
    18781 aaccagaaaa tcgtcgtgga cgcaaacttt atcccggcat gatggttgaa gttccagaag
    18841 taggaacttt ttttatggta aaaaaaggaa acgacgataa tgaggctgaa taagctgtat
    18901 ttaaaaaatt atcgaaatta cgaagagctg aacatcgaat tttccaagaa cctcgtcatc
    18961 tttttaggag aaaatgcgca aggaaaaacg aatcttttgg aaagtatcta cgtattagct
    19021 atgacgcgaa gtcatcggac aagtagtgag caggaattga ttggctggac tgacgatcag
    19081 gcgatgattc aaggagagat cacaaaaggt tcatccactc ttccattaga aatcttatta
    19141 tctaaaaaag gccgaaaaac caagatcaac catatcgaac agaaaaagtt aagtacttac
    19201 gtgggacaaa tgaatgtcat tttatttgca ccagaagatc tttctttagt aaaaggcagc
    19261 ccgcagatca ggcggaagtt tttagatatg gaattaggcc agatcaatcc aatctatctt
    19321 tatgatcttg tccaatacca agcaattttg aaacaaagaa accagtactt gaagcaattg
    19381 aatgaaaaaa aacaaactga tttgctttat ttggatattt tgtccgaaca actagcagcg
    19441 tttggcagca aagtattgaa agcaagagca cagttcgtca agcgattgga gttttgggct
    19501 aattctttgc atcagcaaat cacccatcaa aaagagcagc tggaaatcga gtatctcacc
    19561 gcagtcgatt ctttagaaac acatacgcaa gagcaaatcc aagaacaatt tctcgcactt
    19621 ttgaaccaaa ataaaaagaa agaccttttt cggggaacaa cgacagttgg tcctcatcgt
    19681 gacgatcttt ccttttttat taatcagaaa aatgtgcaga cttatggatc gcaaggacag
    19741 caaagaacaa ctgcactaag tgtcaaatta gcagaaatcg atttgattaa agaagagaca
    19801 ggggaatatc caatcctttt gttagatgat gtgatgagcg agctggatga taatcgtcaa
    19861 ctccatctgc tagaaacaat cgaaggaaaa gttcaaacgt tcttaacaac aacgacactt
    19921 gaccatgtaa aaaataaaat gactgttgaa cctgagattt tttatgttca acaagggaaa
    19981 ctaaaaggag aagaaactga atgacagaag aaagaagttt agtagaacgc gctaaagagt
    20041 atgatgccag tcagattcaa gtacttgaag gacttgaagc cgttcgtaaa cgacctggca
    20101 tgtatatcgg ttcgactagt tctgagggtc tgcatcacct tgtttgggaa attgtcgata
    20161 attcgatcga tgaagtatta gcaggttttg caacgaaaat ccatgtgatt attgaaaaag
    20221 acaacagtat caccgttatt gacgacggtc gggggatacc tgtagatatc caggcaaaaa
    20281 ctggacgtcc tgcagttgaa acggtcttta ctgttttaca tgctggagga aaattcggcg
    20341 gtggtgggta caaagtttct ggtggacttc acggagtagg atcttctgtt gtaaacgcgc
    20401 tttctacaca gcttgatgtc aaagtataca aagaaggaaa tgtgtactat caagaatatc
    20461 gtcgtggagc tgtagtggac gacttaaaga ttatcgaaca aacggatcgc catggaacaa
    20521 cggttcactt tacgccagac cctgaaattt ttacagagac aactgagttt gatttttcaa
    20581 aattggctac ccggatccgt gaacttgctt tcttgaaccg cgggatgcgt atatcgattg
    20641 aagacaaacg agaagaagaa ccagtcatca atgaatacca ttatgacggc gggatcaaaa
    20701 gttatgtcga atacttgaat gcaaacaaaa ccgtgatttt tccagaacct gtatatttgg
    20761 aaggtgaaca gcaagatatc gcagttgaag tctctatgca atatacagac ggctaccatt
    20821 ccaatatcat gagttttgcc aacaatatcc atacgtatga aggtggaaca catgaatcag
    20881 gattcaagac tgctttaact cgtgtgataa atgattatgc ccgaaaacaa aagctgatga
    20941 aagaaaatga cgacaacttg accggtgaag acgtgcgaga aggattgaca gctgttattt
    21001 ctatcaagca tcctgaccct caattcgaag gacaaacaaa aacaaaattg ggcaattccg
    21061 aagtacggac agtcactgat cggttattta gcgaacattt catgaaattc ttgctggaaa
    21121 acccaagtgt gggccggcaa attgttgaaa aaggattact agcttccaga gctcgtctag
    21181 cagcgaaacg tgcgcgtgaa gtaacgagac gtaaaggtgc tttagagatt agtaatctgc
    21241 caggaaaatt agcggactgc tctagcaacg atcctgaaaa atgcgaattg ttcatcgtcg
    21301 aaggagattc ggccggcgga tcagctaagc aaggtcgtaa tcgagagttt caagccatct
    21361 tgcctattcg tgggaaaatc ttgaatgttg aaaaagcaag catggacaag atcttggcaa
    21421 atgaagagat ccggtcttta tttactgcta tgggtacagg gtttggagaa gattttgatg
    21481 tatcgaaagc tcgctaccac aaattagtta tcatgactga tgccgatgtc gacggcgcgc
    21541 atatccgtac attgctgctg actctttttt atcgttacat gcgtcctatt gttgaagctg
    21601 gatacgtcta tattgctcag ccacctttat acggggtaaa acaagggaag aatattacgt
    21661 atgtacaacc aggaaaaaat gcagaagaag agttgaaaca agtcgttgct tcacttcctg
    21721 cctcaccaaa accaagcgtt cagcgttata aaggacttgg agaaatggat gatcatcaac
    21781 tatgggaaac aactatggac ccaagcaatc ggatgatggc ccgcgtcagt gtggacgatg
    21841 ctattgcagc agatcagata tttgaaatgt tgatggggga tcgagtagaa ccccgtcgtg
    21901 cattcatcga ggaaaatgca cactatgtga agaacttaga tatttaatca tgaaggagga
    21961 aaaagaatga gtgaagaaat cagagaaaac atccatgatg tcaatctaac cagcgaaatg
    22021 aaagactcct tcatcgatta tgcgatgagt gttattgtcg cacgtgcact tcctgatgtc
    22081 agagatggtc tgaaacccgt tcatcgccgt attttatacg gcatgaacga attaggtgta
    22141 acaccggata aagcccataa aaaatctgcc agaatcgtcg gagatgtcat gggtaaatac
    22201 catcctcatg gagacattgc gatctatgag tcaatggtac ggatggcaca gccattcagc
    22261 taccgttata tgctagtgga tggtcatgga aactttggtt ctgttgacgg tgacggtgcc
    22321 gccgctatgc gttataccga agcacggatg agcaaaatcg ctacagaaat gctgcgtgat
    22381 atcaataaga acactgttga tttccaaagc aactatgatg atacagaaaa agaaccagtt
    22441 gtactacctg ctcgcttccc gaatctttta gtgaacggaa caacggggat cgccgtaggt
    22501 atggccacga atatcccacc tcataacttg tctgaggtag tagcggctat cgatttgctg
    22561 atggataatc ctgatgtgac gaccaatgaa ttgatggaag tattacctgg accagatttt
    22621 ccaacaggtg gtttggttat ggggaaatca ggaatccgaa gagcgtatga aacaggtaag
    22681 ggttctatta ccgtacgtgc aaaagttgag attactgaga tgccaaatgg taaagaacgg
    22741 atcatcgtga cagaattgcc ttatatggtc aataaagcaa aattgatcga acggatttct
    22801 gaactgcaca gagacaagcg tatcgaaggg atcacagatt tgcgggacga atcttcccgt
    22861 gaaggtatgc ggatcgtcat tgacatccgt cgtgacgcca gcgcatccgt tatcttgaat
    22921 aacttatata aattgacatc cttgcaaaat tcatttggat tcaatatgtt ggcgatcgaa
    22981 aaaggtgtgc cgaaagtttt gagcttgaaa caaatccttg aaaactatat cgaacatcaa
    23041 cgcgaagtca tcacacgacg gacggtcttt gaaaaagaaa aagcagaagc aagagcacac
    23101 atcttagaag gtttgcgtat tgctttggat catatcgatg aaatcattgc gattatccgc
    23161 gggtctaaat cagatgacga ggcaaaagca acaatgatcg aacggttcga tttatccgat
    23221 cgccagtctc aagctatttt agatatgcgt ttacgccgat tgaccggttt ggaacgggaa
    23281 aaaatcgaaa atgaatacca agaactctta aaactgattg aagatttaac tgacattctg
    23341 gcaagaccag aacgtgttac agaaatcatc aagaatgaat tagcagactt gaatcaacga
    23401 tttggtgata aacgacgcac agaattacta gtcggtgagg tcttaagtct ggaagatgaa
    23461 gatttaatcg aagaagaaga aatcgttatt acattgacaa acaatggtta catcaaacgt
    23521 ttggcaaata atgaattcag agctcaacga aggggtggac gtggtgtcca aggaatgggt
    23581 atccacaatg acgacttcgt caaaaatctg gtttcttgtt ctacccatga tacattgtta
    23641 ttctttacga ataacggaaa agtgtatcgg gcaaaaggat acgaaattcc agaatacggg
    23701 cgtacagcca aaggaatccc aatcatcaat ttactaggta tcgattcgag tgaaagcatc
    23761 caggcaatca ttgcggtgac gggagaagca gaagaaggcc actatctctt ctttacgacg
    23821 cgtcaaggta cagtaaaacg tacttctgtc actgcttttg ctaacatccg aagtaatggt
    23881 ctgatcgcaa ttggtttgaa agaagacgat gagctcgtca atgtgctact gacagatggt
    23941 caatctaata tcattattgg aacacataac ggctactctg tcactttcaa agaagaagca
    24001 gtccgcgata tgggacgtac agcttccggg gtcagaggga tacgcctgcg tgaggaagat
    24061 tatgtcgttg gggcagcctt aatgaaggaa aatcaagaag tattagtgat cacagaaaaa
    24121 ggttacggaa aacggacaaa agtatcggaa tatccagtga aaggccgcgg aggtaaaggg
    24181 atcaaaacgg cgaacatcac tgaaaaaaat ggtccactag ctggattgac cactgtttct
    24241 ggagatgaag acatcatgct gattacagat aaaggtgtca ttatccgttt caacgtatcg
    24301 actgtttctc aaactggacg ttcaacatta ggcgtacgtt tgatgaagat ggaagctgat
    24361 acgaaagttg tcacgatggc agcagtcgaa ccagaagctg cagaacaaac agttgaagaa
    24421 caaggatcag aagaataacc atcaagacta agtgtgtaag ctagcaagaa taaccgatta
    24481 aaaaatctgg ttattcctgc tggctttttt gattagttga atttctctta aaatattcga
    24541 aactgttcat tctgtattgc ttttgaccaa aatagagtgt ataattagag cttgtgagta
    24601 gtctaattaa ttagataact ctccttgctc ttaggaacct aagagccgaa agtccacagg
    24661 gaggtgaata tcaatggaaa acacgaaata tgaaatcatg tacattattc gtccaaacat
    24721 tgatgaagaa gcaaaaactg ctttaatcga acgtttcgat gcaatcttga aagataacgg
    24781 agcagaagtt atcgaatcaa aagattggga aaaacgccgt ttcgcttatg aaatgaacgg
    24841 ttaccgtgaa ggtatctatc acatcgttaa agtatcttct ccatcatcag caaatgctgt
    24901 caatgaattt gaccgtcttg ctaaaatcaa cgacgatatc attcgtcaca tgattgttaa
    24961 agaagaagct taattgttgt ttcatgtgaa acaatagaaa acgatgagag gagatgacta
    25021 ttttgattaa taatgttgta ttagttggac gtctgacgaa agatccagat ttgcgttaca
    25081 cagcaagtgg tacggcagtc gcaacattta ctttggctgt gaatcgtaat tttacaaacc
    25141 aaagcggcaa tcgcgaggct gattttatca actgtgtgat ttggcgcaaa tcagcagaaa
    25201 ctttagcaaa ctatgctcgt aaaggaacac ttttaggtgt tactggacgt atccaaactc
    25261 gttcttatga taatcagcaa ggacaacgtg tatacgtgac agaagtagta gctgacaact
    25321 tccaattgct ggaaagtcgt tctgcatctg aaaatcgcca acaaagcggc ggattccaaa
    25381 gttctggtca atcagctggc ggatttggcg gaaacaataa ctctaatcaa acatctcaat
    25441 catccaacgg tatgcctgat ttcgaccgtg atacttctga cccattcggc tcaagttcaa
    25501 cgatcgacat ttcagacgat gatttaccat tctaaacgaa tttgatagga gggaaataga
    25561 atggcacaac aaagaagagg cggacgtaaa cgtcgtaaag tagactacat tgctgctaac
    25621 catattgaat atatcgatta taaagatatc gaattactaa aaagatttat ttcagaacgc
    25681 ggtaaaatct tgccacgtcg tgtaactggt acaggcgcta aaaaccaacg taaaattact
    25741 gttgcaatca aacgcgctcg tatcatgggc ttgctacctt tcgttagcga agactaataa
    25801 atcctaggaa ttgaatgact gatccttttg atcagctatt caattccttt ttttctgtaa
    25861 aaataacctc tattcgttta gtcgctcgaa tgacgaaagt tatggtaaaa tcaaattagt
    25921 catatatggg aggaaaatta tggaaaataa aaacaatcat ttatccccga aacctctctt
    25981 ccttatcctg tttatattaa tcgaagtatt ggtagtctta attttaccat ttcgttttgt
    26041 tagcgcttcg gtcattctat tgatgaattt atggggtgtt cgctattggt tgaaaatgaa
    26101 acgttggctt attttaaata aacgtgaaaa aatccagcag gcttcgactg tagctgaaac
    26161 caatctagct tatgtctcag aagtcatgcc tgtaggagtg attgcttatc agccatcaag
    26221 ccaaaaaatc gaatggctca atccattagc attgaaaatc aaagatcaaa gtggattaac
    26281 agaggaagaa ttgctagata ctttttttac tgcaaaagaa aaaaagaaaa accaagtcta
    26341 tctagcagat aaaacatttc agtttgatat ggatacagta aagggtgttg tttattttaa
    26401 tgatattacc caaaaccagt tgttaaaact ccatcagacg aacagtcagc cggtgattgg
    26461 tattttatct attgataact atgcagatgc cattgataaa ttagatgaca aagagatttc
    26521 ttatttgaac tctttcgtca ctacactagt ttctgactgg atgaatgaat accatgtctt
    26581 tttcaaaaga atcaacgcag aacggttttt cttcgtagca cagatgatcg atctgaaaaa
    26641 gatgatggat aaaaaatttg atttgctgga tcgctttcga gcagaagctg aaaaacagag
    26701 ctcgcctatc acccttagta tggggattgc ttatggctca agaaccctcg atgaaatcgg
    26761 tggagaagct cagaataatt tagatgttgc tttagtccgt ggaggcgacc aagttgtcgt
    26821 gaaagaggta aaagaaggag caaaacctgt ttattatggg gggaaatctg ctaagaatgt
    26881 caaacgcacg cgtgttcgtt ctcgagcgat gagtaccgct ttgcgtcgga tttttgatga
    26941 aacaggcgat gtgtttatca tggggcacaa gtttccggat atggatgctt taggctctgc
    27001 ttttggtgtc agttgtctgg cacgcttcaa ccagaaaaaa gcttacatcg ttatcaatga
    27061 gaacgagatc attccagatg tagaacggtg tcttgaagaa atccataagc atcctgaatt
    27121 agaaatgcaa ctaatctctc cagaacgtgc catagagctg aaaaaagaag aggacctcct
    27181 cgtgatggtg gattaccata aaccttctct atcgatctct cagggcctat atgagcaatt
    27241 tgataagatt gtgatcattg atcaccatcg tcgtggggaa gagtttccaa gtaaacctct
    27301 gctgacttat attgaatctt cagcttcttc agcttcagag ctagtttctg agctgatcca
    27361 ataccaaagc agcaaaacaa atcgcttaga tcgttttgag gccacattat tgcttgcagg
    27421 gatggttgta gatacaaata gtttcaatgt ccgtacaact gcacgaactt ttgacgttgc
    27481 cagctattta aggtcttgtg gagcagacgc atccttagta caatatttat tgagctcgga
    27541 tttagcgaca tacttggaga tcagcaattt gattgctgga agcgactatg tgacttctga
    27601 tattgttgtg gcggcaggga cagacgaaaa agagtataat agtgttatag cagcaaaaac
    27661 agcagataca ttgctttcga tgatcaatat caatgcagct ttcgtcatta caaagcggac
    27721 agacggcttg attgggatca gtgccagaag taccggcacg atcaacgtcc aaatcatcat
    27781 ggaatcctta ggtggcggag gacacttcac aaacgcagct actcaactgg aaaatacgac
    27841 agttgaggaa acaaaacaga agctatttgc tgcaatcaaa gaaaatatga atcaaatata
    27901 tgataaggag taatccaaat gaaagtaatt tttttacaag atgtaaaagg aaaaggtaaa
    27961 aaaggggaca tcaaagaagt tccagcaggt tatgcacaaa attatctaat caaaaataac
    28021 ttggcaaaag aagcaacgaa aagcagtatc agtcagttag caggacaaaa gaaagcacaa
    28081 gaaaaacacg aagctgaaat cttggaagaa gctaaaaagt taaaagaatt cttggaaaaa
    28141 gaagatactg tagtcgaatt gaaggcgaaa gcaggggaag acagccgttt gtttggttcg
    28201 atcccttcaa aacaaattgc tgaagggtta aacaaacaat acaaaatcaa attagataaa
    28261 cgaaaaatcg aattagaaaa tccgatccgt tcactaggct acacaaacgt acctgttaag
    28321 cttcatcatg aggtgacggc tacgatcaaa gttcatgtcg tacaagaata acaaatcttg
    28381 atagcgatgt tcagctggta agtataacca gaaggaaaaa agacaaaccg aaatttatct
    28441 gagaaaacag ctgcagcaat gaaagcaaaa gctgcaaatc gatttcagat tgatttcggt
    28501 ttcttttttg ggaacagcaa aatagcataa agaaaacgag tataataaaa gagaactatt
    28561 tttttattac actttcggta cgaatgatga cttttctctc attttcttgt aaaatgaagc
    28621 aattgaagac aaggagcgat acgtatgaat gaagtgtggc aggatcgaat cccaccgcaa
    28681 aatattgaag ctgaacaagc ggttttaggc tctgtttttc ttgacgcaga aacgatcatt
    28741 gatgccatgg agtacataga gccaaaagac ttttatcgtc gtggccatca aattattttt
    28801 gagacaatga tcgagttaaa tgatcgtaat gaagcgattg acgtggttac tgtcaaagac
    28861 cggctggaac aagccaattt gttagaagat gccggtggtt taagttatct ctcagacttg
    28921 gcattagcag ttccgacagc agcaaatatc gtctactatg cgaaaatcgt tgaagaaaaa
    28981 tcattgctgc gtacattgat ccagacagca acaggaatcg ttacgaaagg attcgagcaa
    29041 ggagaagaag ttcaaacaat tttagatgat gccgaacgca gtatcttgga ggtttcggaa
    29101 aaaagaaatc gaagcggctt tttatctatt gctgaagtac tgaactccac gattgaaaat
    29161 atcgatcgac tagctcaaaa caatgaagaa atcacaggtc taccaactgg ctatcaaggt
    29221 ctggataaaa tgacagcagg tttccagccc gaagaactaa tcattcttgc tgcacgtcca
    29281 gcggtaggta aaacagcgtt tgcattgaat attgctcaaa atgtaggaac aaaaacagat
    29341 cgttctgttg cgatatttag tttggaaatg ggggcagaat ctcttgtaaa tcggatgctt
    29401 tgtgcagaag gttcgattga ggccagccat ttacgtacag gtcagctttc ggaagaagaa
    29461 tggtcgaatc tgatcgttgc gatggggagt ctttctcgag caaatatttt tatcgatgat
    29521 acaccaggga tcaaaatctc tgaaatccgt gcgaaatgtc ggaaactagc gcaagaaaaa
    29581 ggcaatctag gcttgatatt gatcgattat ttgcagttga ttgaaggtaa cggtaaagag
    29641 aaccgtcaac aagaagtatc agatatctct cgccaactga aaaaactggc gaaagagctg
    29701 aaagtacctg tcattgctct ttcacagtta tctcgtggag tagaacagcg acaagacaaa
    29761 cgaccagtgc taagtgatat tcgtgaatcc ggatcaattg aacaagatgc ggacatcgta
    29821 gccttcttgt atcgagatga ttattatgaa agagaaggcg gagatgacga tgatggaaac
    29881 gagccaaaaa atgacaacgt catcgaagtc atcatcgaga agaaccgaag cggtgcacga
    29941 ggaacagttg aactactgtt tatcaaagaa tacaataagt tttcgtcgct ttcgccaaga
    30001 gaagaatttt aaagaagagg ttgtgagcct tgagcaatcc cgagagctct cgtaagaaga
    30061 tgcaaggagt aaccgcagga acgactagag tacttaattt aaacagatct ccaaagggat
    30121 tgtgacaaaa gtcttgtcac agtccctttt tccgaataaa cggtgttcaa tcagctgacc
    30181 cttggtatta gctcagcaaa cgtcaaaata tgaggaacta ttttgacgtt tgtttctcta
    30241 atactcaggt caaaacgctg atcttacgac ctcttttcct taaacggtgt tctcctgcca
    30301 actcctcggt cacaagtcac aatatcatca aacatgagaa gcagtttttt gaaattgctc
    30361 cttgtgactc agagttaaac ggcaggctca caacctcttt cttaaacggt gttcaaaagc
    30421 ttgcatctgc ggtaatacgc ccaatcaaac gaaaaatatg agaagctatt tttgtttggc
    30481 tgttcttatt acttgatgca ggacagcttt ttcacaacct ctgattcacg gtgttccaaa
    30541 agtagcttct tcgaaaaata agtcgaaatt tcacaaaaat ttgagaagca attttcgtac
    30601 attccttctt atttcttgaa gctgaccact tctgttacaa cctctttttt aataaccatt
    30661 ctaaaaagat aaagttcgtt ttttaagcgt ttttataaaa taaaaataaa aatcattcga
    30721 ttttatttga aatatgtatc tattattgat acaatggttc aggattaaat aatatagaac
    30781 gaggtgttca catgtcatct gtagtagtag taggtacgca atggggcgat gaagggaaag
    30841 gaaaaatcac agactttctc agtgaaaatg ctgaagtgat tgcacggtac caaggtggcg
    30901 acaatgcagg gcacacaatt aagtttaacg gtgtgactta taaactgcat ttgattcctt
    30961 ctgggatttt ttataaagac aagatcagtg tgatcggaaa cggcgttgta gtcaatccta
    31021 aatcattggt taaagaatta gcttatttac atgaaaaagg tgtagaaaca agtaatttgc
    31081 gtatttctga ccgcgcgcac gtaatcttgc cttatcatat caaattggac caattacaag
    31141 aagatgctaa gggcgaaaat aagattggta caacgattaa aggaatcgga cctgcctata
    31201 tggataaagc agctcgtgta ggtatccgta ttgcagactt attagataaa gaaatttttg
    31261 aagaacgtct tcgtttgaat ctagatgaca aaaaccgtca atttgtaaaa atgtttgatt
    31321 cagaaccact tgattttgac gatatttttg aagaatatta tgaatacgga caacaaatca
    31381 aacaatacgt cacagatact tccgttatct tgaatgatgc tttggatgca ggcaaacgcg
    31441 tgttgtttga aggcgcacaa ggtgtaatgc tggatatcga ccagggaact tatccttttg
    31501 ttacttcttc taacccagta gccggtgggg taacaatcgg aagcggtgta gggccttcaa
    31561 aaatcaataa agttgtcggc gtctgcaaag cctatacgtc acgtgtcggt gatggaccat
    31621 ttccaactga attatttgat gaaacaggag aacaaatccg cgaagtagga cgagaatacg
    31681 gtacgacaac tggtcgtccg cgtcgtgtag gctggtttga ttctgtagtt atgcgccatt
    31741 ctaaacgagt ttcaggtatt accaacctat ctttaaactc gatcgatgta ttaagtggat
    31801 tggaaacagt aaaaatttgt acggcttatg aattagatgg cgaactaatc taccactatc
    31861 cagcaagctt gaaagagttg aaccgctgca aaccaatcta tgaagaattg ccaggctggt
    31921 cagaagacat tacaggatgc aaaacacttg cagaactgcc agaaaacgca cgtaactatg
    31981 tacgtcgtat ctcagagctt gttggtgtac gtatctcaac attttctgtc ggacctgata
    32041 gaacacaaac aaacatctta gaaagcgtct gggcgcaaat ctaaggataa aaacgacgac
    32101 tgaatcagga cgttcttacc agagcgagtc gaagtgttta tctatacgat aataataact
    32161 tgaagaggta gtgaaagaga cgtttagcct tattgccgat aggctgtctc ttgaacactg
    32221 tttattcaga ataagggact gtgacaaaaa ccgtgtcaca gtcccttttt ccgaacaaac
    32281 ggtgttcaaa agcttgcatc tacggcacta agcccaatca gatgaaaaat atggagtgct
    32341 atttttatct gattgttctt actgcttgat gcagagcagc tttttcacaa cctctccact
    32401 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa
    32461 gctgtttttt gaaattgttc cttgtgactc ggagctaaac ggcaggctca caacctcttg
    32521 gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
    32581 aactattttt tccgtttttt tctctaatac tcaggtcaaa acgctgattt cacaacctct
    32641 gattcacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaatttg
    32701 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
    32761 cttattttga ttagaacttg cgaaaataaa ccggttaaag tgtgtctagc gcaagagatt
    32821 tgctataata gacgataagg gtaaaatgta acgagaaggg gttgaaaaat gtgtatcaaa
    32881 ttgtaacaga ttcttgttgt gatctgccat accatgtatt agcaaaagaa aaagttgatt
    32941 ttttatcaat gaatctccaa ttgaatgatc aagagttagt agatgacctc ggaaagacat
    33001 tcaattatga ccgctacatg aatcagctga aaaatggtgc gatgcctacg acatcacaaa
    33061 tcaatgttgg ccgatattta gaatttttcc gttcttatgc ggaaaaacag attcctgttt
    33121 tatatgtggc tttttccagt ggattaagcg gctcttacag tagtgcgctt caagcggttg
    33181 aattgctgaa ggaagaatat gaagaccctg agatctatgt ttttgataca aaagctgcaa
    33241 gtattgggga agggctttta gtattggaag ctgtaagatt gaaaaaagaa ggtaaaagca
    33301 tcgaagaaac cctagagtgg ctggaaagta attatttgaa agtccattca tgggtaacag
    33361 tagacgatct gaaatatcta gaacgaggcg gtaggatttc caaagcaagt gctactcttg
    33421 gcagtctatt gagtgtaaag cctattatta cggtggataa agaaggaagg ctgacgaatg
    33481 ttgacaaagt tcgaggaaga aacaagtcga tccagacaat cgtcaatgag acggtaaaaa
    33541 caatcgttgc accacttgat caaatcgttt atgtggctta cgctggggat ctagagagcg
    33601 cggagaaggc aaaacggctt ttggaagagc agataaagat gaaagatgtg aagctttatc
    33661 ccttaggacc aacgatcgcc agtcacacag gctatggctg tatcgcgatt ttttcgatgg
    33721 gaatcagtcg ataaaagcag taactcattc tgtaaaaaag cccagaaatt gattgtaaca
    33781 aaaacagcga ctcttaaaag atagatgagc atcctatctc ttagaggtcg ctgttttttt
    33841 attttttaag ctaaataggc cattatgccc ataactcgtt caattgtttt tgaatatctg
    33901 cattttccaa aaattcatca tatgtcgtat cagctcgatc gatcacacct ttatccgaaa
    33961 cggcaatgat gcggtttgcc aatgtttgga taaattgatg gtcatgtgac gcaaataaga
    34021 ttgagccagt aaatgccatc aagccgtcat tcaatgctgt gattgattct aagtctaaat
    34081 ggttcgttgg atcatctaat acaagtacgt tagctttcga aagcatcatt tttgaaagca
    34141 tgcaacggac tttctctcca ccagataaaa cattcactgg tttcaatact tcttcaccag
    34201 aaaacaacat tcttcctaag aagcttctta agaaggtgtt atcatcttct tctttacttg
    34261 catattgacg caaccagtct aaaatcgtta gatcagtatc gaattctttc gttgtatcct
    34321 ttggtaaata agaattactt gttgtcacac cccaacgtac tgttccagta tctggcgtaa
    34381 tttcacccat gataactttg aatagcgttg ttgttgttat atcattttct gcgacgaaag
    34441 cgaccttgtc ttctttgctc aaattaaaag atatatcatc taaaattttc tttccatcaa
    34501 tcgttacact tacattttct acctgcaata agtcgtttcc aatctcacgt tcaggtttga
    34561 atccaacgaa tggataacgt ctagaagaag gttggatatc gtctaaagta attttttcaa
    34621 gcattttttt acgtgaagtt gcttgttttg attttgatgc attcgcacta aagcgggcaa
    34681 taaattcttg gagttctttg atttgttctt cttttttggc atttgcgttt gcttgaagtt
    34741 tagccgctag ttgactagat tccaaccaaa aatcataatt tcctacataa agtttgattt
    34801 taccaaaatc tagatccgcc atatgtgtac atactttgtt caaaaagtga cggtcatggg
    34861 atacaacgat cacagtattt tcaaaattga tcaaaaattc ttccagccaa ttgatcgatt
    34921 ggatatccag accgtttgtt ggttcgtcta gcaaaagaac atcaggttta ccaaatagtg
    34981 tttgtgctaa taacactttt accttttgtc caccagttaa ttcactcatt tgctgatggt
    35041 gcaattcttc agggatgttc agaccttgca gtaaaacagc tgcttctggt tcagcttccc
    35101 atccattaag ctcagcaaat tctccttcta attcagctgc tcgaatacca tcttcgtcag
    35161 tgaaatcctc tttcatgtaa atcgcatctt tttctttcag cacttcatac agacgcttgt
    35221 gtcccatgat gactgtttca agtacagtat agtcttcata gtcgtaatgg ttctgtttca
    35281 atacggccat ccgttcatct ggtcccatag taacggaacc tgtggaaggc tgcagatccc
    35341 ctgataacac tttaaggaac gttgatttac ctgcaccatt tgcaccaatc aagccatagc
    35401 agtttccggg ggtaaattta atattcacat catcaaatag tttgcggtct gaaaagtgca
    35461 aactcacatc atttacagta atcaattttt ttcctcactt ttatcattat tagttttgac
    35521 aagtcttata gaattatata ggggaaaggt aaaagattca aggcaatcga acattctttg
    35581 tgtttcacgt gaaacagaat gaaaaaaata aaaataaaag cggagcgaaa atagctacta
    35641 taggatttat tagtttgcaa cagcataatt tttgacgtta tttataagag ggagtactat
    35701 aaaactgtaa aagaaatgaa agaaggattg aaaatggatg aaaaaagaac catcgactgg
    35761 ggctcattga ttttgggaat tttatttgta cttgtctcat tgttatcatt tcaagaccct
    35821 gtcggaaatt tagttgcaat tgtagtagtc tttgcgattt ttgcctttat taaagggata
    35881 tttgaactgt ttgtacggaa cagattaaaa gagctaacag gatataaagg aaagatgccc
    35941 ttaataatcg ggatcatcga tattctagtt ggcgtctttt tcttattcaa tattggtgca
    36001 ggagtcgcag cattgccttt tgtttttgca gtatggttta ttgctgattc agttttagcg
    36061 ttatttactg ctgacttggc aagaggagta agtgaaggat attattggtt tacgatcatc
    36121 gtcaatattt taggaatttt attaggaatt ttcttattgt ttaatccaat ttcttctgcg
    36181 ttgacattaa gctttttagt tggattctat tttatgttat ttggtatcac acatatcgtc
    36241 tatgcattta gataagtaaa atcaaacaaa taaaaaagcc ggcttccgat tacagtttta
    36301 ctgttgtaat tggaagccgg cttttttatc atggacaaat atgcacgagg tgatttttag
    36361 gtgtcgagac aaatttatgc aaaaatgtga gaagctatat acacaaattt tatcttaata
    36421 ctcgaagcat agcgcagttg tcacaacctc ttttcatata attcattcaa tactggcaaa
    36481 tcatggactt catatttttc tttgaatgct tggatttctt cttcagaata ttttcttgga
    36541 tctaaatgca gatcttctac tggcaatggg cgcttgttat tgatcttcac atcgattact
    36601 acaggacgat ctgattttgc agctgcatca aaggcaaatc gaagttgcga gtattctgtg
    36661 actgtaaagc tatttgcccc gagcgcttct ccaactttac cgaaatctgc atcttctaaa
    36721 aatacaccaa atttcttttg ttccgtatct tcttgttctg cttcgatgaa accaaaagaa
    36781 tcattcgaaa agacaacatt aataattgga agctgatatt tgacttgtgt aatgatatct
    36841 tgcatgacca tagcaaaccc gccatcacca cttaaggtaa atacttgttt ttctgggtag
    36901 cttagttgtg cggcaattcc tcctggcaca ccattaccca tagttgcgaa ccatccagat
    36961 gtcgtatgtt tttgtttacc gttcatattc aataaacgaa tagaatggat agtcgtgtta
    37021 ccgacatctg tgacgaaaat cgcatcgtct tcagcaatac gattgatttc tttatagaca
    37081 gcctctggac gaatcggttc ttcctctcga tcttcaaaag atttcaacca atttacccat
    37141 ttttctttat tttcttgatt tgctaggtac cagctatcag cagggcgagc atcccctaac
    37201 tcgacaagtc tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga tgtttcatgg
    37261 cgtcgtccaa attttgacgc atcgatatcg atttggataa attcagcagt tggattaaaa
    37321 aatgcacgtc caaatgggaa gtcgctaccg acaaataaga tcagatcggc ttctgccaat
    37381 gcttcatttc cgggtttagt agcgacacgt ccagcaaaac caagaaagtt ttcataatca
    37441 tcaggaataa ttccttttgc tagaacggaa gcgacaacag gaactgaaaa atgttctgag
    37501 aattctttga tttgcggaaa tccgtttcgc gttccttgac caacataaag aactggtttt
    37561 tttgcttttt caagataagg caaagcagct agcaaatctt tttcttctgg taatatcaca
    37621 cttgttttgt gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc aaaaccaaga
    37681 tcaacaggga tagtcacgat agccacgcct ttatgctcat aggcggcttt aatggcttca
    37741 tctactacat gcggaagact ttgtggtgtc atgactgtac gattgtatac gctaacatct
    37801 gcgaaaatag gattttcatt tagttcctgg aaagagttat agttcatgga tgtagaagca
    37861 acttgtccta agaacgctac tagaggcaca tggtccatct gtgcatcata caaaccattg
    37921 atcaaatgtg tcgcaccagg tcctgcagag ccgaagacag cgccgacttt ccccgttaat
    37981 tttgcatctg cagcggctgc gagtgcgccg acttcttcat gacgaacttg aatgtagttg
    38041 atttcggttt tttcttgata tagcgcatcc ataattgaat tgaaagatcc tccgggaatg
    38101 ccgtaaatat ggtcgatacc ccagctttca agaactttta ccattgcgat tcctgcattg
    38161 atttttgaca tggtgtttca ctcctttagc aatgatgata cataaatttt ttgtatctag
    38221 agtatagaac tttttatcga aaatcccaac taattcgtct tttcacaaaa atgtagtcat
    38281 gtttttggga atatgctaga gtagtacata gatagattca agagagggga atggcatggt
    38341 tgaatggcta ttgagacttg aaccttggca gcaagcactt gctgggacag cttttactta
    38401 ttttatgaca gctcttggtg ccgggttggt ttttttcttt aaagaaatca aaaaagaagt
    38461 gctaaacttg atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt tttggtcttt
    38521 gttagatccg gctattacaa aagcagaaga aaatggagat attgcttggc tagttgtaag
    38581 tattgggttt ggtttaggcg gtgtgttttt atatatggca gataaaacac tgccccatat
    38641 gcattttgga ccacaacatg aagcagaagg attgcctacc catttaaaac ggacgatctt
    38701 gcttgttttt tctattacat tgcataacat tcctgaaggt ttagccgtgg gggtagcatt
    38761 cggtgcagca gcaactgcgg ataatccgac agccgctata ttagccgctg tttcggtggc
    38821 gctaggtatt ggtatccaaa acttcccaga aggagcagct gtatcaatcc cgttgcgaca
    38881 agaaggtctg agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa tcgttgaacc
    38941 aattgctgga gtgattggag cgctactagt aacgaaagtg gagctgctgc ttccttatgc
    39001 acttgctttt gccgcaggag caatgattta tgtcgtggtg gaagaattga tccctgaagc
    39061 acagcagaca ttgtccagca aacgtcattt tgcagttttt ggcgtcatga gtggatttat
    39121 cattatgatg attttggatg tggcattagg ataaataaaa aaaatatata tgatatatca
    39181 aatagggccg cgaacttata tcgcgaccct atttttttag taagttatat cataatgaag
    39241 tgaatggtat catacattaa taatagtttt ttctatgtgt atttcctttg atccagcgaa
    39301 caagccagat gataagtgcg attgggatta gaactttgac cgcaaaccat aaaagcccgc
    39361 ttaggacact gacaaaaaga ctgatgacga agctagctgc aattagcact aaaagaacca
    39421 tgaagatatt taagaacata tttttttgac gcatacaaaa ccctcctctt gttcattctt
    39481 tactatagaa aaagtataac agggattgtt taaaaacacc tccaactgaa gactgatttt
    39541 tacttcatct acttaaatga tcaaacaatt gctttttctc tggtttatca tctaaaatag
    39601 agcggaaaag gaggagaata tcatgcctaa aaatcgtctt gaagcattta cagatgctgt
    39661 aatcgcgatt atcatgacta ttttagtttt agaacttcat gaacctaacg gagacacgtg
    39721 ggaggcgctc gctgcattag gttaccgatt ttttatctat attgtcagtt ttatcatttt
    39781 agcgatttat tggaacaatc atcatcattt atttcaagtt gtacataaga taaatggacg
    39841 ggtcttatgg gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc
    39901 atggatcagt gagtacatca atagcctggc tccagaaatg acttatggtt ttgttacctt
    39961 gaccgctaat attgcgtatt atatcttggc aaaagaactt gtcaaagcaa ataaaggcca
    40021 cgagaaagca ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat cgattggttt
    40081 gaatgtgtta ggaattgtct tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa
    40141 catcgtgatt ctagctgctt ggatcatccc aagtcgtacc atcgaatcac aatatcggta
    40201 aaatgatccg tgtttaaaat ttaagagaaa aatatagaaa aaataaataa acgatcaaat
    40261 atccgagtga tactagtatt caaagaggga tgcagatgga tcaaaaaaga attcatgtag
    40321 gactgattac tagttttgtc acgatgctta tttatttgga tcaaggtcat ttgacttgga
    40381 cagtagttgg tgtcgtagga tttttgacat tattcacagc aataaaaaaa taaacatctt
    40441 tatgtaatct gatgttctat ctatttaaga aaagcatgtt tacagcagta tgattctgtt
    40501 gtaaacgtgt ttttatttgt ggaaaattta atttttagaa aagtgtatcg aaaaatagat
    40561 agttagtttt tccacagtgg ataacttcgt aagagataaa aataaaaaat tagtcaaaga
    40621 agggcgtata gatttctttt gataggagat atacaacaga aatgaataaa tattaacgtg
    40681 ttttcaaaca gtataaaacg gtgtggtttt tggaataaaa aaataacata tctcatttta
    40741 cgcttttgtt attttatact taaaaaataa cgacacagaa aataccgtgt ctcagatttc
    40801 tctactactt ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa agtaggatca
    40861 ggcagaatat actggcagaa tcaaggagaa gggagcctaa ataaggagaa aaaccataga
    40921 aaatataaag taattactta taaaacaagc tttttataga aaaataatat acctgagtag
    40981 ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa gaatgttaag
    41041 attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt
    41101 cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga tgaaaataga
    41161 tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg aatgtgtcta
    41221 ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc aaaaataaaa
    41281 aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga cggtctaact
    41341 tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc agtcctcctg
    41401 ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg aattgaatca
    41461 attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa gaaagtctct
    41521 tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta taagtggtct
    41581 cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc
    41641 tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa atcaaccata
    41701 atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata gacatctcct
    41761 ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa
    41821 ggaccaacgt agttatcgta cgaaaattag aagctttttt ttgttgggaa tccggtttct
    41881 ttaacaaagc taagatagcg agttgcttgg aaaagttgct atcttttttc tgtcaaaacg
    41941 actcggaatg ttcgtttttt gttctaattt ctccaagttg tttacttttt gatactaagt
    42001 ttatgaaaaa tcaagaaata gttgttatac taggtaatgc tgacaaacgg gattggcatt
    42061 tcatcttggt tcagcttttt ttccaattac taggaaggag ggatagattg ttcaagactt
    42121 attttcgttt caataaaata caagataatc cttttcgtat catgaatctg atgctactta
    42181 tctttattgt attatggtta ggttacataa atcatgattt tttgatttca agtttttcat
    42241 cattagggat ctttactttt ttctattatc aaaacatacc gatgaaatat ttgctgaaac
    42301 gtatgtttct gatcggttgc ggactgttgg ttgctttcgt tttggggatc ttatcgactt
    42361 atgttttatg gcttgaacca tttgccgtag ctttggttgc tttttccagc agattcgtgt
    42421 tgcgattatt ccatatttct aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg
    42481 gaacgagcat gcagctgcca atcgctcaat taccgatcgt ctcactttat ttttttatgg
    42541 gtgtcgtttt tgccctgatt gcggcagtga ttacaaaatt actggattca cggcctgagc
    42601 agacaattga aaaagcaact ttaaaagaaa gatttcacga agagccttta gtgatcattg
    42661 attctgtttt ctacagcgct gccttatttc tttccgttta tgtcagtcac ggcttgaatc
    42721 tgcataatcc ttattggttg accttgtcat gtgcatcgat tttgctagca gaaaacttgg
    42781 atgcgatgaa gcatcgacaa gtccaatatc tgattggatc gatgggtggg ttatgtgtgt
    42841 cggcattctt gtcatttgtg ccgtttacgc agttgcagac catttttctg atcacttttc
    42901 tatatggaat cgcacaattt ctagttgcta gaaattatgc agttgctaat atttttctta
    42961 atccgatggc gttgatgcta tctacactta ttcgtggagc atacttgatt tctttgatcg
    43021 aatatcgttt tttagggatc gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa
    43081 tgacggttgg tttgcaacat tatttaacgg ttgttcgtca aaaattagaa tagaaggaca
    43141 aatatatgtg ccaaaagagg ttatgacaag atttttgtca caacctctaa ttcaaggtgt
    43201 tcaaaagcca gattctcagc attaagtcaa attcacacaa aaatgtggaa aggaactttc
    43261 aaaaaaattt tatatttatt ctatcaaaaa attttatatt tattctatca aaaaattttg
    43321 tttatgatgg taactgaata aactttcctg agatggtcaa tgctttcctt gaaagtctta
    43381 cagatattct ttcttcatag aaagtttaag ttttatagtt agtgtacaat aaaagtatgt
    43441 ttataccata tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa agatatgaaa
    43501 aggggaaatt tgatgaagaa aataagcaat gaaatccaaa ttctggcaga aactttaact
    43561 cttcccaaag tcgttataga gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg
    43621 tttgagcaat atagtcatga actaatggat cctaataaag caaaggcgac tgcaaagaaa
    43681 atagacaaag tctatgaaga ggaacctttt gcagcattag ctatttatct atatgcagct
    43741 tctcaaagtt ggaaaaaaat ttatcaacca ttgaagattc caagaactat ctatttggca
    43801 acaatgaatg cttttactag atttattcat gaacatttcc aagtaacagg aagctatcgc
    43861 tttgaccgtg gtttttggat ttggcattac actagtggct tgatctttcg gattggtgaa
    43921 ttggagtacg aaatgacata ttttccgaaa gggtacacgg atctaagatt agaaggaaag
    43981 cagtgccttt ctcttcatat tccttccgat gcagatttat ctcagaataa gataagagat
    44041 agttacgaaa gtgccattcg tttttttgaa ctttattttc ctgattacca ttaccaagtg
    44101 atgtacacag atacttggtt actatcaccc aatcttacta aatggttgaa aaaagagtca
    44161 aaaatctgtc tttttgcagc cgattatcga ttattatcag ttgatgaaca ggacgatagt
    44221 ggagtccctt ggatctttgg acgagtagat gcccaaatac atgattatcc agaaagtacg
    44281 agtttacagc gacaagcaaa agagcaattg cttgcaggcg agcacattgg ctctggctta
    44341 ggtattctta cttcataagt ttagtacttc gaaatagttt aaaagagcgt gagacaaaag
    44401 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
    44461 cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt
    44521 atttcttgga gttaaacgct tctgtcccag cctctttttt agtgttctat cgaacacaca
    44581 gtacgttcaa taggtaaact agctataaca atagatatta aagttaacgg acaagagggc
    44641 cttttttagg tagctaaaac gtaataatga aaatttattt ccagagatag gagtcaatca
    44701 gctagtctga tcgactgttt taacaagttg actttcattt taacttttga taccatttaa
    44761 aacacaaatc gtaattgttt cgatttgtgt tttttagcat gactacaatc aaagaagaaa
    44821 gtgggtcgtc catatggaaa aaacagatat ctcaagtgca taccgccgct tgaaaagccc
    44881 aaatatcaaa acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg
    44941 gaaaaggtaa gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt tttcggctat
    45001 tttcaataat cggtttttgt gtaaggtttc cgaaagaact gctgttgatt ttccattttt
    45061 aacatatgat aaattggtag aatctttctt ttcaaattga taccattccc taattccagt
    45121 tatatagtta actacagaac cacaaagcac gtacgtttat aaaagtatta tctgtagtta
    45181 tttttcaatt ccctttcaac ctcttatttt gaccagtagt attgatcact ttacctttta
    45241 acacctttgt tcgtttttga aacattttta gtcaagaatg gaaacgtaac tacataatca
    45301 aaggttttgc gaaatatttc atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa
    45361 taaccattat cgtttaataa gcagttacga aagctcccaa tatctctaac gttcagctca
    45421 ctccatttct tttcttcaaa aaaatcagtg attcgtatct ctttctattt gtcgaaaaag
    45481 ttaactttaa aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg
    45541 ctagaataac atcaaggaat aataagtttt ggttttcaga taatagtcga taagttgtgt
    45601 attggaattt tagatacagt aataaagtat ttttcatgaa attttgtgag aatcactatt
    45661 acaagtatct ttaataaaat taatggttaa ggaggaactt aaagtagaat gtaaatgaat
    45721 agttgtagtc agtcgattgt gatactagac tcaaagttgc aggaataagt ttttcaattt
    45781 gtgattggaa gataaaaaat gaaaaagaag ggcttttttt tatactttgt tgattgtagt
    45841 gtttattttc cctctgttta gccaagtaac attttctatg actcaaccag tttcaagtga
    45901 ggaacatgat aacctttttc gtgctacaga agagtttaag aaatatcagg aaaatattgt
    45961 tccggagact ccattagcta gtgagccaat tactgatatt gatggaaacc aaataggatg
    46021 gtatatacag tacgaagtta tggtggtaaa taatgactca atagtaaaaa atacagtagg
    46081 cttagagcta acaccgattg aaaaggtgag ttcattgtta acgtatattt atactgatga
    46141 aagtgacgag actgagatca ttttaacgga ttactcaaaa attgctactg ataaaaagta
    46201 tatattgtgg atgtagacac tagtgaaata ctttatgata tagaaattga tgaaaattta
    46261 ctagatgtag cacaagaaat cgatcatcaa aagcaggaag ctattcgata tgctggtaga
    46321 aaagggaaaa gaaaatattt attacgtgaa tgtagcaaaa gttagagaag atgaaaatga
    46381 gtggaaagaa ttcaagagtc gctactcaat taactcgact ccgacattta cagtttacag
    46441 agaaggatcg atagaaaaaa ctgttttttg gactaaagaa agtggaatgt ctttagctga
    46501 agtagaagag tttttggatt atgttagtat gcaacagtaa gggagttctt attatgaaaa
    46561 attttgacac atattatgtt attgtttttt cgatatcttt gctgttaata ttactaactt
    46621 taatgcagta taaagagtat ccagagtatt ttattttaac ttttgtatat ttagtaagta
    46681 gctacacact tctatataaa ttcagaagtt cttctagagt ttctaaagtt tgtttcttaa
    46741 ttagcttatt gattctataa tatttttgat ttatatagga aattaaaaat attatagaat
    46801 caacactcag agaacgggtt ggtttttaat agatttaata agtactgata atgaaatttc
    46861 tcctggttgg gaaaacgaag tttcccaaac aaaaggatat taagaaataa cctgtgttta
    46921 cctgcttgca aattattgta ttttggataa gcaagagaaa aactttttat tgacagaaat
    46981 aatatcaaat attataattg tccgtaattg aataactatc ttcagggcag ggtgtaattc
    47041 cctaccggtg gtacagccca cgagccgaaa ggcatgattt ggtgtaattc caaagccgac
    47101 agtatagtct ggatgaaaga agataaaagt aagtttatgc ttttttgcgt gctctggaat
    47161 gattttatat tgtttcagag cattttcttt aatcgcataa atcctctttt gcatgtcttg
    47221 gatagatttt attcaaggca tgtatttttt tggaggtgta aaaatgagcg atatagaata
    47281 catgaaaata gcaatagaac tggcaaaaaa gggtgctggc tatgtcaatc ctaatcctat
    47341 ggttggagca gtaattgtaa aaaataatcg gcttatagga cagggatacc atgaagtttt
    47401 tggtggatta catgcagaga gaaatgcact aaaaaattgc agagagtcac ctgtaggagc
    47461 aacgctttat gtaacacttg aaccatgctg tcactatggt aagacaccac cttgcacaga
    47521 aacaattatt aaaagtggta ttacaagggt agtcgtcgga actttagact gtaatccgat
    47581 tgtgtctgga aaaggtgtaa agcaacttga ggaaaataac attcaagttg atgtaggcat
    47641 tttagaaata gaatgtcaac agttaatcaa agtatttagg aagtatatta caaagcgtat
    47701 tccgtatgtt tttatgaaat atgcaatgac aatggacggg aaaatagcaa cttatacaaa
    47761 tcaatcaaaa tggataagtg gcgaaaaagc gagaaaacaa gtgcaccaat tcagacataa
    47821 ggttactgca atcatggtag gagtaaatac cgttattcaa gataaccctt tactgacatg
    47881 ccgattagaa aacgggaaaa atcctattcg tatcgtatgt gatacagatt taagaacacc
    47941 cattacatca aaaattatcc aaacagcgaa tgacattaaa acatatatcg ctacttcttc
    48001 tattgatgaa agtaaaattg ctctttacag aaaatgtggt tgtgaaatta tttatacgaa
    48061 gaaaaaaggt aatcatattg acttaatgaa tttaatgcaa tgtttaggaa atatgcagat
    48121 agacagtctg cttttagagg gtggaagtgc gttgaattgg agtgctcttg agcaacagat
    48181 tgttgatgag gtacaaactt atatagcacc taaaatattt gggggtagtg gaaaaagccc
    48241 tgtaagtggt caaggtgttg tttttcctaa agacgccatt atgctcaaac catatgcttt
    48301 ttctcaagtg ggaaacgatt atctcataga aagtgaagtg atttatccat gtttacagga
    48361 ataatagaag aaatcggtaa agtagaaaga atacagaaag atacttataa ctgtaaacta
    48421 tccattaaat cttcaaaaat attagaggat atacatttag gcgacagcat agcagtcaat
    48481 ggtatctgcc ttacagttac ttgtttcaca cgacaatctt ttatagttga tgtgatgaat
    48541 gaaacatgga acagaacggc tcttaacctg ttaaaacatg gaagccttgt gaatttagaa
    48601 agagccatgc atatgaatgg cagattgggc ggtcacattg ttacggggca tattgacggg
    48661 acaggcagaa tatcgtctgt gcgtagagac aaaaatgcgg tgtggtatca aatcaatacg
    48721 caaagagaaa tcttaaatct aattgttgaa aaaggttcgg ttactattga tggtattagt
    48781 cttactgttg caaaagtatc gaaagcagat ttttcagtat ccgttatccc tcatactttg
    48841 gaacaaacaa ttctcaagaa taaacaagtc ggggatatgg taaaccttga aaatgatata
    48901 ttagggaaat atgtgaagaa actcatagga aacagcaatg aaacagagtt gtcgaaagag
    48961 ttgttatgcc gatacggatt ttagcagaaa ggataaagga attatgaaat ataaaaaaat
    49021 aaaagaggct ttgcaggcgt tacaagaggg aaaacttgtt ttagttatgg acgataaaga
    49081 gagagaaaat gagggggatt taatttgctc ggcacaattt gctacaacag aaaatgtaaa
    49141 ttttatggcg acttatgcaa aaggtttaat ctgtatgcct atgagtgaaa gtctggctaa
    49201 caggcttatg ctttccccta tggttgaaaa taatacagat aaccatgaaa ctgcttttac
    49261 agtttctatt gattataagg ataccacaac aggtatttct gccgaggaaa gaggattgac
    49321 agctcgtatg tgtgtggttg aggatataaa tccctttgat ttccgtagac cggggcacat
    49381 gtttcctctg attgcaaaga acggaggtgt tttagaaaga aacggacata cggaagcaac
    49441 gattgactta ttgaggttgg caggattaaa agaatgtggc ttatgttgcg aaattatgaa
    49501 tgaaaacggg aaaatgatga gaacatctga tttaattcaa ttttcccaaa tacaccatat
    49561 accaaccctc acaattaaag aattacagga atacagaaaa gtatatgaca tgcttgtaga
    49621 atgtgtttca gttgtagaaa tgcctacaaa atacggggat ttcaaagcac attgttatat
    49681 caataaattg aatggcgaac atcatgttgc attggttatg ggagagttaa atagtggaaa
    49741 tgatgtgcta tgtcgtgtcc actcagagtg tttaacagga gatgtctttg gctctttacg
    49801 ctgtgattgt ggacagcagt tagatagagc aatgaaaatg attgcagaaa atggttctgg
    49861 cgtattgctt tatatgcgac aagagggacg aggtatcggg cttgtcaata aactaaaagc
    49921 ctatcattta caggataagg gtatggacac acttgacgcc aaccttgcac taggatttca
    49981 aggcgattta agagaatatt atattggagc acagatttta agagatttgg gaataaaatc
    50041 attgcattta cttacaaata atcctgataa agtttatcag ttagaagatt atggaatgca
    50101 aatttcaagc agagtaccga ttgaaataaa agcaaatcct tatgatagtt tttatttaaa
    50161 gacaaagaaa gaccgaatgg gacacatttt gaatatggag gaaaaataga tgaacacttt
    50221 tgaaggaaat ttagtagcag aaaatataaa aattggtatt gttgtagcaa ggtttaatga
    50281 atttataact tctaagctat tagcaggtgc attagataat ttgaaaagag aaaatgttga
    50341 tgaaaaggac atagaggtag cttgggttcc gggagcattt gaaataccta tgatagcgtc
    50401 taaaatggta aaaagtaaaa aatatgatgc aattatctgc ttgggagcag ttattcgagg
    50461 tagtacaagc cattatgatt atgtgtgtag tgaggtatca aaaggaattg ctcaaatcag
    50521 tttaaatact gaaacaccag ttatgtttgg tgttcttacg acagatacga ttgaacaggc
    50581 gatagaacga gctggcagta aggcaggaaa taaaggctct gaatgtgcac agggagctat
    50641 tgaaatggta aatctaattc gtgcattaga ggtataatgt tgatcagcac attataatat
    50701 gccattttca gagaaataaa tagtgcagaa ataatacggg gatcttccat ccccgaaccc
    50761 ctggaatcct tgttataaca gtaatagaaa aaattattac cgttataaca aggctaaaaa
    50821 tgggatgttt taattttccg aattctgcaa attatggcat caaataaaat gcgttgtaac
    50881 agtataatat gcttggggtt aaaagctaaa gcgaaaaaac ataacaggcg gagtgatcca
    50941 gacacgacgg tgggaaaatc tcttagattt ttccgccgtt tttagtggct ggttcacttc
    51001 ggcagttgag cgagcttgcg agcgaacagt aagcccccgt tatctaacaa ggggggcaca
    51061 aaaaacaaga gacaatagac actactaagc ggaaaacgct gtatttacaa ggcaaaaccg
    51121 cttttaacag ggtgttaact aaaagacact catttaacac ataaaattga ataagacata
    51181 ggaaagacag ttgatttcaa atgaaaaatt gattgtcttt ttcttttgaa gaaatttaag
    51241 tatagacaat tatcagtaaa tggtagttgt cttttttgat tggaggaatt gaagaatgaa
    51301 tgataagaac tttatagaag aattaagaca aaagcgtgaa gaatacggag taacacaaac
    51361 aagaattgct gttgcctgtg gtatcagtcg tgagtattat aaccgcattg aaaaaggcaa
    51421 acagccattg aataacgaat taaaggaaat catagaaaaa cagattgagc gtttcaatcc
    51481 acgagagccg ttatttttac taattgatta ctttcgtgtc cgctttccta ctacggacgc
    51541 attaaagatt attcgtgatg tattacaact aaaagccgat tatatgcttt atgaagattt
    51601 tggaaagtac ggatatgaaa gtaaatatgt tttaggcgac attaacatca tgtgttccat
    51661 gcaagagcat ttaggtgttt tgttggaact gaaaggcaga gggtgtcggc aaatggaaag
    51721 ttatctcttg gcacaggaac gctcatggta tgactttatg cttggttgta tgacggcagg
    51781 cggtaagctg aaacggcttg acttggcaat caatgataag gcaggtatct tggatattcc
    51841 aaaactaata gaaaaatata aggctggaga atgtatctcc tattttcgca aacagaaaga
    51901 ttacggtggt acagagaaaa atgggtatga tacaccacaa aatacaggcg aaaccttata
    51961 tctcggttca acaagtagcg aattatatat gtgtgcttat cagaagaatt atgagcagta
    52021 tgtcaagaat ggcacagaag ttgaagatac agaaatcaag aaccgttttg aaatacgcat
    52081 gaagaatgaa cgagcctatt atgcggttgt agatttactg acttatcggg acgctgaacg
    52141 tactgctttt tctatcatca atcattatgt ccgttttgta gatagagagg acgataaacc
    52201 gaaaagtcaa tggaaaatga atgatgattg ggcgtggttt gtaggagaaa acagagagcc
    52261 gatacgctta accacaaagc cagagcctta tactttacaa aaagcattgc attggctaca
    52321 aagacaggtt gcaccaacca taaaaatggt acaggcatta gacaaagaaa atcatacaac
    52381 gatactaaaa gatatgattg aacaggcaga actcaaagac aagcataaac atttattaca
    52441 gttagaaaaa tcaaccatag aagaacgcat agataccgtt gttccacaag agaatgacgg
    52501 tattttttaa tttctttaaa aaatctgcaa caaaacatca actagcgtac agatatggta
    52561 cgagaaaagg aaatctaaac ttttttgaaa ataggtcatt aaatcgaggt ttccattccc
    52621 tatatgaagt gaaagaagaa aagttttgaa aaatgtcgga aaatcgagtt aagcggacag
    52681 tgttatgtga aaagaagaaa tttttttcat tctcaactta acaatcagca gtttccattc
    52741 cctatatgga gcaaagaaaa tctttcattc catagttggc agtaacggtg ttgcatgggt
    52801 agttagggtg tgaaaagaaa tcttttcatc atcaacttag cagaatgaca ttttctttcc
    52861 ctatatggta cagaaaaaga aaaaagtttt gagtattggt tacgcttttg ctcttttcca
    52921 acgcggtata taggaaagac gattttcttc tttcgtgttc tttgacaatt gaataaagca
    52981 attcaataca ttagacagac agtaagccat tgaaacagat acgccatgac ctttcccctg
    53041 caaaagcgag cgacaaccta tctgtgttct tgtaaaagac ttgtcctctt ttatcgccat
    53101 gacctgtcct gtctgataat gatactcccg tacagccaca gcttgagcgt taaaagcgtc
    53161 gcacgcaatg ggtacggcta cattggataa tgcagaggtg gaactcctgt gggctatgac
    53221 aaaagctgtt gaattgctta gaaagatttt acagaaaggg gggtatgtgt tattgataat
    53281 gctgtaaaaa caattcaaaa gaaaaataac aagtgtggca ttggaaacag aggaaagaca
    53341 aagattgttg taaaagagca tttttccgaa aagggaaaga caatggaaga acttttaact
    53401 gatgttatgc ttgagaaagc aaagcagaca atcgcataaa ttcggtgcag ttgtaagaaa
    53461 tagattgaat ggcaaaaagt acccgtgtta tactttactt gcaagcaggt attgtaaaca
    53521 cgggttagtt ataaaggagg ataactgaca atgaaacaac agatttacaa tactgcactt
    53581 tatcttaggt tgagccgaga tgatgaatta caaggcgaaa gttccagcat taccacacaa
    53641 agaagtatgt tgcgtctata tgcaaaagaa catcatttga atgtcattga tgaatatatt
    53701 gatgacggct ggtcgggaac aaattttgaa agaccgagtt ttcagagaat gattgaggat
    53761 atagaggtag gaaaaatcaa ctgtgttgta acaaaagacc tttcccgact tggcagaaac
    53821 tatattatga caggacaata tacagaattg tattttccca gccataatgt ccgttacata
    53881 gcgattgacg acggtgtaga cagcgaaaaa ggcgaaagtg agattgcacc atttaagaat
    53941 attatcaatg aatgggtggc aagagataca agccgtaaag tgaaatcagc ctttaagaca
    54001 aaatttgcag agggtgcgta ttatggggct tatgctccgt taggatataa gaaacacccc
    54061 gacatcaaag ggaaactgtt ggttgatgag gaaacaaaat ggattgttga aaaaatcttc
    54121 tctctcgcct atcaaggtta cggtagtgca aaaatcacaa aaatactgcg agaagaaaaa
    54181 gtcccgacat cgtcttggtt gaattttaca aggtatggta cttttgcaca tatttttgag
    54241 ggcaaacccg aaagtaagcg ttatgagtgg acgattgcac aaatcaaagg aattttgaaa
    54301 agtgaagttt atatcggcaa cagcgttcat aatcgacagt caacagtatc attcaaaagc
    54361 aagaagaaag tgcgaaagcc cgaaagcgaa tggtttcgag tagaaaatac ccacgagcca
    54421 attattgaaa aagaagtgtt ctatcgtgtt caggaacaga taaaatcaag gcgtagacag
    54481 acaaaggaaa aggcaacgcc gatatttgca gggcttgtta agtgtgcgga ttgtggctgg
    54541 tctatgagat ttgcaacaaa taaggcaaat aagacgcctt acagttatta tgcttgcagt
    54601 ttctatggac aatttggcaa aggctattgt tctatgcact atatccgcta tgatgtgctg
    54661 tatcaagctg tattggagcg attgcagtat tgggctaagg cagtacagca ggacgaagaa
    54721 aaggtattgc ataagataca gaaagtcggt aatgccgagc gaatacggga aaagaagaaa
    54781 aaggcaagtg ccttgaagaa agccgagaac cgacaaaatg agattgactg tttatttgcg
    54841 aaaatgtatg aagatagagc ctgtgagaag ataacagaac gaaacttcat tatgctgtcg
    54901 ggaaaatatc aaaaggaaca gatagaactg gaacagcaga taaccgactt aagagaagaa
    54961 ttaagtaaaa tggaacagga tatgataggt gctgaaaaat ggattgagtt aatcaaggag
    55021 tattccgtac caaaggaact gacagcaccg ttattaaatg caatgataga aaaaatactc
    55081 attcacgaag caacaacgaa tgaggagaac gaaagaatac aggaaataga gatatactac
    55141 cgatttattg gaaaagtaga ctgataaata gggttcatat ctttaactaa gggagacgga
    55201 agttggttac cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat
    55261 aatgagagat tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa
    55321 tgaattatct gataaggcga aagaagaagc agttgccatt attt
//
LOCUS       DAPBCS010000012        54695 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPBCS010000012 DAPBCS010000000
VERSION     DAPBCS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.012"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            322..630
                     /gene="rpsJ"
                     /locus_tag="RD521_000897"
     CDS             322..630
                     /gene="rpsJ"
                     /locus_tag="RD521_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDT7770647.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            661..1290
                     /gene="rplC"
                     /locus_tag="RD521_000898"
     CDS             661..1290
                     /gene="rplC"
                     /locus_tag="RD521_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDT7770648.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1318..1941
                     /gene="rplD"
                     /locus_tag="RD521_000899"
     CDS             1318..1941
                     /gene="rplD"
                     /locus_tag="RD521_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDT7770649.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1941..2231
                     /gene="rplW"
                     /locus_tag="RD521_000900"
     CDS             1941..2231
                     /gene="rplW"
                     /locus_tag="RD521_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDT7770650.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2270..3103
                     /gene="rplB"
                     /locus_tag="RD521_000901"
     CDS             2270..3103
                     /gene="rplB"
                     /locus_tag="RD521_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDT7770651.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3146..3424
                     /gene="rpsS"
                     /locus_tag="RD521_000902"
     CDS             3146..3424
                     /gene="rpsS"
                     /locus_tag="RD521_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDT7770652.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3447..3794
                     /gene="rplV"
                     /locus_tag="RD521_000903"
     CDS             3447..3794
                     /gene="rplV"
                     /locus_tag="RD521_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDT7770653.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3808..4464
                     /gene="rpsC"
                     /locus_tag="RD521_000904"
     CDS             3808..4464
                     /gene="rpsC"
                     /locus_tag="RD521_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDT7770654.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4467..4901
                     /gene="rplP"
                     /locus_tag="RD521_000905"
     CDS             4467..4901
                     /gene="rplP"
                     /locus_tag="RD521_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDT7770655.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4891..5079
                     /gene="rpmC"
                     /locus_tag="RD521_000906"
     CDS             4891..5079
                     /gene="rpmC"
                     /locus_tag="RD521_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDT7770656.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5104..5370
                     /gene="rpsQ"
                     /locus_tag="RD521_000907"
     CDS             5104..5370
                     /gene="rpsQ"
                     /locus_tag="RD521_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDT7770657.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5428..5796
                     /gene="rplN"
                     /locus_tag="RD521_000908"
     CDS             5428..5796
                     /gene="rplN"
                     /locus_tag="RD521_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDT7770658.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5833..6141
                     /gene="rplX"
                     /locus_tag="RD521_000909"
     CDS             5833..6141
                     /gene="rplX"
                     /locus_tag="RD521_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDT7770659.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6169..6708
                     /gene="rplE"
                     /locus_tag="RD521_000910"
     CDS             6169..6708
                     /gene="rplE"
                     /locus_tag="RD521_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDT7770660.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6727..6912
                     /locus_tag="RD521_000911"
     CDS             6727..6912
                     /locus_tag="RD521_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDT7770661.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6948..7346
                     /gene="rpsH"
                     /locus_tag="RD521_000912"
     CDS             6948..7346
                     /gene="rpsH"
                     /locus_tag="RD521_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDT7770662.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7378..7914
                     /gene="rplF"
                     /locus_tag="RD521_000913"
     CDS             7378..7914
                     /gene="rplF"
                     /locus_tag="RD521_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDT7770663.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8075..8431
                     /gene="rplR"
                     /locus_tag="RD521_000914"
     CDS             8075..8431
                     /gene="rplR"
                     /locus_tag="RD521_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDT7770664.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8452..8952
                     /gene="rpsE"
                     /locus_tag="RD521_000915"
     CDS             8452..8952
                     /gene="rpsE"
                     /locus_tag="RD521_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDT7770665.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8967..9146
                     /gene="rpmD"
                     /locus_tag="RD521_000916"
     CDS             8967..9146
                     /gene="rpmD"
                     /locus_tag="RD521_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDT7770666.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9190..9630
                     /gene="rplO"
                     /locus_tag="RD521_000917"
     CDS             9190..9630
                     /gene="rplO"
                     /locus_tag="RD521_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDT7770667.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9630..10925
                     /gene="secY"
                     /locus_tag="RD521_000918"
     CDS             9630..10925
                     /gene="secY"
                     /locus_tag="RD521_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDT7770668.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10987..11634
                     /locus_tag="RD521_000919"
     CDS             10987..11634
                     /locus_tag="RD521_000919"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDT7770669.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11825..12043
                     /gene="infA"
                     /locus_tag="RD521_000920"
     CDS             11825..12043
                     /gene="infA"
                     /locus_tag="RD521_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDT7770670.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12076..12192
                     /gene="rpmJ"
                     /locus_tag="RD521_000921"
     CDS             12076..12192
                     /gene="rpmJ"
                     /locus_tag="RD521_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDT7770671.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12210..12575
                     /gene="rpsM"
                     /locus_tag="RD521_000922"
     CDS             12210..12575
                     /gene="rpsM"
                     /locus_tag="RD521_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDT7770672.1"
                     /translation="MARIAGVDIPRDKHVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12603..12992
                     /gene="rpsK"
                     /locus_tag="RD521_000923"
     CDS             12603..12992
                     /gene="rpsK"
                     /locus_tag="RD521_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDT7770673.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13072..14010
                     /locus_tag="RD521_000924"
     CDS             13072..14010
                     /locus_tag="RD521_000924"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDT7770674.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14038..14418
                     /gene="rplQ"
                     /locus_tag="RD521_000925"
     CDS             14038..14418
                     /gene="rplQ"
                     /locus_tag="RD521_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDT7770675.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14690..15529
                     /locus_tag="RD521_000926"
     CDS             14690..15529
                     /locus_tag="RD521_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7770676.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15505..16374
                     /locus_tag="RD521_000927"
     CDS             15505..16374
                     /locus_tag="RD521_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDT7770677.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16364..17161
                     /locus_tag="RD521_000928"
     CDS             16364..17161
                     /locus_tag="RD521_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDT7770678.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17364..18110
                     /gene="truA"
                     /locus_tag="RD521_000929"
     CDS             17364..18110
                     /gene="truA"
                     /locus_tag="RD521_000929"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDT7770679.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18565..21234)
                     /locus_tag="RD521_000930"
     CDS             complement(18565..21234)
                     /locus_tag="RD521_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDT7770680.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(21472..21660)
                     /locus_tag="RD521_000931"
     CDS             complement(21472..21660)
                     /locus_tag="RD521_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDT7770681.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21930..22292
                     /locus_tag="RD521_000932"
     CDS             21930..22292
                     /locus_tag="RD521_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDT7770682.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            22344..24026
                     /locus_tag="RD521_000933"
     CDS             22344..24026
                     /locus_tag="RD521_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDT7770683.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            24143..26179
                     /gene="recG"
                     /locus_tag="RD521_000934"
     CDS             24143..26179
                     /gene="recG"
                     /locus_tag="RD521_000934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDT7770684.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            26228..27229
                     /gene="plsX"
                     /locus_tag="RD521_000935"
     CDS             26228..27229
                     /gene="plsX"
                     /locus_tag="RD521_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDT7770685.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            27351..27593
                     /gene="acpP"
                     /locus_tag="RD521_000936"
     CDS             27351..27593
                     /gene="acpP"
                     /locus_tag="RD521_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDT7770686.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            27861..28865
                     /locus_tag="RD521_000937"
     CDS             27861..28865
                     /locus_tag="RD521_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770687.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            28866..29810
                     /locus_tag="RD521_000938"
     CDS             28866..29810
                     /locus_tag="RD521_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770688.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            29810..30772
                     /locus_tag="RD521_000939"
     CDS             29810..30772
                     /locus_tag="RD521_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770689.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            30799..31713
                     /locus_tag="RD521_000940"
     CDS             30799..31713
                     /locus_tag="RD521_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770690.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            31739..33520
                     /locus_tag="RD521_000941"
     CDS             31739..33520
                     /locus_tag="RD521_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7770691.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            33868..34554
                     /locus_tag="RD521_000942"
     CDS             33868..34554
                     /locus_tag="RD521_000942"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDT7770692.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            34574..38155
                     /gene="smc"
                     /locus_tag="RD521_000943"
     CDS             34574..38155
                     /gene="smc"
                     /locus_tag="RD521_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDT7770693.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            38164..38973
                     /locus_tag="RD521_000944"
     CDS             38164..38973
                     /locus_tag="RD521_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7770694.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            38984..39982
                     /gene="ftsY"
                     /locus_tag="RD521_000945"
     CDS             38984..39982
                     /gene="ftsY"
                     /locus_tag="RD521_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDT7770695.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            40169..42436
                     /gene="gshAB"
                     /locus_tag="RD521_000946"
     CDS             40169..42436
                     /gene="gshAB"
                     /locus_tag="RD521_000946"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDT7770696.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(42525..42977)
                     /locus_tag="RD521_000947"
     CDS             complement(42525..42977)
                     /locus_tag="RD521_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDT7770697.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            43127..44077
                     /locus_tag="RD521_000948"
     CDS             43127..44077
                     /locus_tag="RD521_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDT7770698.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            44070..45029
                     /locus_tag="RD521_000949"
     CDS             44070..45029
                     /locus_tag="RD521_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDT7770699.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            45026..45781
                     /locus_tag="RD521_000950"
     CDS             45026..45781
                     /locus_tag="RD521_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7770700.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            45804..46757
                     /locus_tag="RD521_000951"
     CDS             45804..46757
                     /locus_tag="RD521_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7770701.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(47401..47697)
                     /locus_tag="RD521_000952"
     CDS             complement(47401..47697)
                     /locus_tag="RD521_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDT7770702.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            48052..48408
                     /locus_tag="RD521_000953"
     CDS             48052..48408
                     /locus_tag="RD521_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDT7770703.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            48418..49317
                     /locus_tag="RD521_000954"
     CDS             48418..49317
                     /locus_tag="RD521_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDT7770704.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            49833..50564
                     /locus_tag="RD521_000955"
     CDS             49833..50564
                     /locus_tag="RD521_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7770705.1"
                     /translation="MMKKEIVLTAAIMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            50709..52736
                     /locus_tag="RD521_000956"
     CDS             50709..52736
                     /locus_tag="RD521_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDT7770706.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            52844..53941
                     /locus_tag="RD521_000957"
     CDS             52844..53941
                     /locus_tag="RD521_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7770707.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            54248..>54695
                     /locus_tag="RD521_000958"
     CDS             54248..>54695
                     /locus_tag="RD521_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770708.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        18280 a         9663 c        11734 g        15018 t
ORIGIN      
        1 ttatttattg caggatcagg aaaaaagaca ggagtattat aaatagaaga aacacgaaat
       61 aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa
      121 agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat
      181 tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc gacacgcccg
      241 gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa
      301 ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat
      361 gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga
      421 gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt
      481 gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt
      541 atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca
      601 tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc
      661 atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt
      721 gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca
      781 atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta
      841 tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt
      901 cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat
      961 gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa
     1021 ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat
     1081 cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt
     1141 gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat
     1201 gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt
     1261 actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg
     1321 ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa
     1381 gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt
     1441 gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga
     1501 cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca
     1561 caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt
     1621 cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac
     1681 aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa
     1741 gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac
     1801 tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg
     1861 agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa
     1921 attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg
     1981 aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa
     2041 acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga
     2101 acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa
     2161 aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag
     2221 ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa
     2281 aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat
     2341 cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa
     2401 caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt
     2461 gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga
     2521 tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat
     2581 cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat
     2641 caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat
     2701 tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt
     2761 acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat
     2821 cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa
     2881 catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt
     2941 aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa
     3001 agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc
     3061 taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt
     3121 cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga
     3181 tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa
     3241 aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta
     3301 tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg
     3361 tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg
     3421 ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg
     3481 caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta
     3541 aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca
     3601 ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg
     3661 aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc
     3721 ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg
     3781 tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt
     3841 gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc
     3901 ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta
     3961 tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa
     4021 ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac
     4081 aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat
     4141 gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc
     4201 gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa
     4261 gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact
     4321 gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca
     4381 tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa
     4441 aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg
     4501 aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat
     4561 acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta
     4621 tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca
     4681 aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag
     4741 gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag
     4801 aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg
     4861 taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc
     4921 actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc
     4981 caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt
     5041 gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag
     5101 cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa
     5161 atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa
     5221 cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc
     5281 gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg
     5341 gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat
     5401 gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct
     5461 gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa
     5521 actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt
     5581 gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt
     5641 acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa
     5701 agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc
     5761 atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg
     5821 tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac
     5881 aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa
     5941 ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt
     6001 gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta
     6061 gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc
     6121 ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta
     6181 aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct
     6241 gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta
     6301 tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa
     6361 ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca
     6421 attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt
     6481 tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt
     6541 cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt agattatgat
     6601 ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa
     6661 gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg
     6721 aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca
     6781 caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat
     6841 ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa
     6901 gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag
     6961 atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt
     7021 tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt
     7081 tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa
     7141 aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt
     7201 tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa
     7261 tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg
     7321 gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg
     7381 agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga
     7441 aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc
     7501 aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg
     7561 aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa
     7621 ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca
     7681 aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta
     7741 actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt
     7801 ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt
     7861 attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt
     7921 agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa
     7981 aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa
     8041 atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa
     8101 aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc
     8161 acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc
     8221 gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac
     8281 agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa
     8341 agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga
     8401 agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat
     8461 atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa
     8521 gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac
     8581 ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca
     8641 attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac
     8701 gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct
     8761 ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc
     8821 acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt
     8881 ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa
     8941 ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta
     9001 ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta
     9061 acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc
     9121 acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt
     9181 gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac
     9241 cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga
     9301 caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg
     9361 ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt
     9421 aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt
     9481 gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta
     9541 aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa
     9601 gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag
     9661 taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag
     9721 gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc
     9781 catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct
     9841 caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca
     9901 tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc
     9961 aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg
    10021 gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa
    10081 cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca
    10141 cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat
    10201 tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca
    10261 tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat
    10321 tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag
    10381 ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag
    10441 ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta
    10501 gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac
    10561 ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc
    10621 aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg
    10681 tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag
    10741 ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg
    10801 atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt
    10861 tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg
    10921 agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg
    10981 aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa
    11041 aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg
    11101 atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta
    11161 gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag
    11221 aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca
    11281 atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa
    11341 atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat
    11401 aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc
    11461 tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa
    11521 aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt
    11581 gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat
    11641 tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga
    11701 atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg
    11761 ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact
    11821 gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa
    11881 tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa
    11941 aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc
    12001 atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact
    12061 acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa
    12121 gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa
    12181 cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc
    12241 gtgataaaca tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta
    12301 aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg
    12361 aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc
    12421 gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc
    12481 atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag
    12541 gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact
    12601 tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta
    12661 ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa
    12721 atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc
    12781 cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa
    12841 agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat
    12901 tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg
    12961 gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca
    13021 gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag
    13081 ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt
    13141 gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta
    13201 tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc
    13261 tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta
    13321 gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct
    13381 acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg
    13441 tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga
    13501 cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca
    13561 gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt
    13621 ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa
    13681 ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg
    13741 aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa
    13801 gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt
    13861 ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg
    13921 atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac
    13981 ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg
    14041 agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca
    14101 actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc
    14161 tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa
    14221 gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc
    14281 attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa
    14341 ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt
    14401 gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag
    14461 tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata
    14521 tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat
    14581 gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt
    14641 ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat
    14701 tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa
    14761 agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc
    14821 aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt
    14881 gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg
    14941 aatgattttt caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc
    15001 ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc
    15061 acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg
    15121 gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt
    15181 agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa
    15241 gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc
    15301 agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc
    15361 aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc
    15421 tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac
    15481 agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat
    15541 cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa
    15601 aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat
    15661 ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag
    15721 ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag
    15781 tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa
    15841 aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt
    15901 gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt
    15961 agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca
    16021 acggcaggac ttgatcccca gggcagaaaa gaaatgatgg agatgttttg gcgtcttcat
    16081 aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt
    16141 gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag
    16201 gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag
    16261 tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa
    16321 gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat
    16381 atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt
    16441 attagccagt ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct
    16501 tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt
    16561 tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt
    16621 gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg
    16681 tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct
    16741 actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc
    16801 attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg
    16861 ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg
    16921 tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat
    16981 tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag
    17041 aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga
    17101 cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt taagaggata
    17161 aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgca
    17221 agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct
    17281 tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa tcattattcc agataacctt
    17341 ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgcg tacgacggaa
    17401 caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa
    17461 aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag
    17521 atgccggagt acatgcgatc gggcaagtaa tccattttga ttatccagag gagcgtccgt
    17581 tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag
    17641 tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat
    17701 ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc
    17761 cttacccact tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg
    17821 atttcacctc tttttgtgca tcaggaagct cgatagagga taaaatacgt acgatctacg
    17881 aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg
    17941 gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc
    18001 gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac
    18061 ctacagcaca ccctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttatga
    18121 tagaagtggt cagcttcaag aaataagaag gtatttacga aaattacttc tcaaaatttt
    18181 gcaaaatttc ggcttatttt cgaagaagct acttctggaa caccgtttaa ggaagaggtc
    18241 gtgaaatcag cgttttgacc tgagtattag agaaacaaac gggaaaatag ctcctcatat
    18301 tttgccgttt gttgagctaa taccgaaggt cagctgattg aacaccgttt aagaaagagg
    18361 tcgtgagcct gtcatttaac tctgagtcac aaagagcaat ttcaaaaaac agcttctcat
    18421 ctttgatgaa tatcgtgact tgtggacgag gcgttgataa gtgaatcatc tttttgttac
    18481 ctattttttc tgtaaccaaa aaacgatgga acgttgtttt gattgccctt ggtgcggaca
    18541 gtctgaacag gttccatcgt ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg
    18601 ttgttcttct attgctggat caatcgtgcc ataaaagggg cgattgcggt caaggtacca
    18661 gacataaaga ataaacaatg caatacatag gacaaaaagg attgcaccta tcgtaaatcc
    18721 ttcaggcaag taactgaaag tgacagtatg tttacctgct ggaactttta tcatcaagaa
    18781 tgcgtctttg aaattgctga ccttgactgg tttcccatca attttagctt tccatccttt
    18841 gtcataagga atagttgtca ccaacatgga atcttcagcg gtatttacag tcccagaagc
    18901 ttttctgcca gtggcttcca tttcgacacc tttttctttg atctgattca ttgcggcttg
    18961 ataggctaca acatctagac caacgacttt aggctccatg aaacttattt ctttcgtccc
    19021 ataaaaactt gcagtaaatg tcactgttgt ctcttgagga taatagccaa gatcatagta
    19081 ttgaccagaa atgttgattt gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact
    19141 agaactttct aattgcgcaa aattggttgg gaacaaactt aagtaagctt gtgtatttgc
    19201 aggcacatgg acttcccaag tgagctcttt tggctggtta tctgctgttt ctgagtaagt
    19261 gacgccagtc gttgtctttg tgatcgtcgt attcaactga ttcttcaaag taggtgtata
    19321 gaatgtaaaa tactgttcat ttagaccaga caatgtattg atcaagtttg tctgactggt
    19381 caaattatct gtcactggct gctcaacatc attaacggac aacggtgcag taaatgctag
    19441 tggcaatgca ttgacattct gatacagtgt atagtcgccg ctttgaccag cttggcggaa
    19501 gccatattta ctgaacgtgc tttcgttttt agaaatgtta tatttgattc ctgtaaaccc
    19561 atccatcagc aaagtattat tgttataacg gatattcaaa tttgtccctc ttgaacggaa
    19621 acctaattta tccaggtaag aggaagaatt acgatttctt atagaagaaa acaaactgat
    19681 accactgtaa ccatagttga tcgaatcgtt tgcagatact ggatccagat tttccaagcg
    19741 gtaaaacgag tcggaatctt tttttgtttc atttacaaga gctttcaagt caggataagg
    19801 atcagtatat aaactgcggg aagcataatt ccaatcatca agaatgccat ggatcatact
    19861 gttcgtattg accagtgctt cagcagacat gatcaataat aacaatatac tcagatgatt
    19921 gaatgaaagt tttttatgct gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac
    19981 cactgtcaat acaaaggaag tcatcgtcac ataggtgtag cttccttcag gtttgatgcc
    20041 ccatgccacc gcaaagagag cagctacgac caaaatgatt cctaacaaca gcccacgatc
    20101 gtcttgttct atcttttccc atccttttcc tgcaagcagg agcactaaga aagagaacag
    20161 ataggcataa cggaataaaa acatattcgg tgcatgcata ccatgccaga acaaattcaa
    20221 tggaacaaga taaaaacttg cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt
    20281 tttcaacggt actttccgac tggcaaaata aaacagacag aggactaatg gtagcagacc
    20341 tgcgtaaacg aacggaatcg atccgtattt tgtcgtgtca taaacaccaa tcatattttt
    20401 catgattaga tccagataag aagtcgcttc tgttttcaac gtagtgatct gactcagtgc
    20461 ctcgccattt gaccgaagat ccagcactgc tggcaataca atgatcattg aagcgccgcc
    20521 tgccagtaaa gaagtgatac cataaggaac aatcgttttt ttatattctg accagtttgt
    20581 caatagacgg gcgataaaat ataagaaaga aaatacgcca atcataaaac ccatataaaa
    20641 actggataaa aacaagagta aataactgat aaataaaagt ttcggccgtt taagatccat
    20701 aacacgatga atccccaaaa tcactaatgg aagataaaca aatgcatcca gccacatgat
    20761 gatctccgaa tgtgcagtga taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg
    20821 cccccagcga ggaatcttga acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc
    20881 gaccttaagc aaagtcagaa catacagtgc atctggcatc atctggtttg gaaaaaagaa
    20941 caccagtggc gtgaagatcc cgcctagata gtaagaaatt aaagataaat aattcaatcc
    21001 tagagaagca ttccatgtat aaaaaatact ttgctttcca agaagaacat ttcggaagct
    21061 ggcatgaaaa ttagaaaact gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc
    21121 aggatagatt ccgatactta gataaacaag tgctaaaatc aaaaacggaa tcaggaaact
    21181 agctgctgcg tagcgtccgt tttctctaaa gaatgacttt aaattttttt tcatatttat
    21241 tttccaccaa atttctatga agttgtattc agtttatcat atccttgttt ttctctgctt
    21301 aaaaagagtc tagttttcac aaaaaaataa aatttagaat aaataacttc ataatttgtt
    21361 cgtataacaa aaataaacgc aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt
    21421 tttattaaat aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg
    21481 ttcaactttt cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc
    21541 gaccataacg cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc
    21601 gtgtgaacgg ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat
    21661 ggtaatttcc tcctttgcct tgatttggag caatagaata attcagctca taccagacta
    21721 acatatcata tagaatggga atttgcaagt acattttcat agatacaatt gacaattgag
    21781 aagaataatt gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt
    21841 tgggtaactg ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg
    21901 agttttttac ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa
    21961 ccattgaaat taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt
    22021 tcggtgttgt aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc
    22081 gtaaagaaaa ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag
    22141 atgtttatac gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag
    22201 aaaaagtgaa atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct
    22261 ttgtacaagg tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt
    22321 cgatctgaag gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg
    22381 aaatggtcca ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa
    22441 atgtattccc tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg
    22501 gagctaaggc agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt
    22561 caaaaggatt gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc
    22621 gtggattttc taagcaaatt ccagatgttg tctcattaaa tgcttctgat cttgctgcag
    22681 ccttcactca tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga
    22741 ttttgacagt agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg
    22801 atgtcgttga agtaatgaca gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc
    22861 cagacttgct tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc
    22921 tgtttgttta tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat
    22981 atgaaccttc tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc
    23041 aaggtcaaat ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa
    23101 tcggagaagg acctacagtc gatagtacat tcgattacga agaattccgt aactatttag
    23161 atggtatagg agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc
    23221 atacagaaaa cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa
    23281 tcaaagtcga caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga
    23341 acgagtttgc gaaaaaagaa ataccacgta tcccgtatgg tatcgtagcg atcgctgctg
    23401 gtaaaggcgt ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtggac
    23461 aaacaatgaa tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata
    23521 aagtgattgt cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag
    23581 tagcagatat cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta
    23641 tgctttcatt caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt
    23701 tgtctagcgt tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg
    23761 tagagatcaa agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc
    23821 cagatatcac agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga
    23881 tcgtaacgat cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga
    23941 aactaacaga aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct
    24001 atccttatct attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt
    24061 cattcataaa aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg
    24121 ttaaaggagg gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg
    24181 agtcggagaa aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt
    24241 actgacttat tatccttttc gctacgaaga tattcaggaa agaaatctaa acgagatcca
    24301 ggatcaagaa aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt
    24361 tggttataaa aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt
    24421 ttcttttttt aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt
    24481 atacggtaaa tgggatgcaa agcgtaaagc cttgaacggg atgaagattt taggttctca
    24541 aacaatggat gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt
    24601 agttgatcta atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc
    24661 aaacaacctt gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca
    24721 ttttccaaaa gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt
    24781 ctttttattt caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg
    24841 tttggagatc ctttatgatg tccaaaaatt gaaacaattt actcaaaagc taccttttga
    24901 actgacaaat gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca
    24961 gcatatgcag cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat
    25021 tgcgttatac gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat
    25081 cttggcacaa caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat
    25141 cgcattgatt acggggtcaa cgaaagcaaa agaacgacgt gagctccttg aaaaactaga
    25201 gcaaggggaa attaacgtcg tgatcggcac acatgcattg atccaagatg gagtcgaatt
    25261 tcagaattta ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa
    25321 agtacttcgt gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc
    25381 tagaacatta gctattacag catatggtga gatggatgta tctatcattg atgaacttcc
    25441 agctggacgt atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact
    25501 ggattggacg aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat
    25561 tgaagaatca gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga
    25621 actatttgca ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa
    25681 agaagtaatc atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt
    25741 aattgaagta ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg
    25801 atttggattg gcacagctgc accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc
    25861 ctactgtatc ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat
    25921 gacagagacc accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg
    25981 tgaagtattt ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac
    26041 ggatggaaat gttttggagg tagccagaga agaggctgca gccctttggc aaataaagga
    26101 ttggcagatg cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga
    26161 taatcgttat ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt
    26221 ggaaacaatg aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt
    26281 agaaggcgtg gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa
    26341 agaagcagaa atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga
    26401 cgaaaaaatc aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc
    26461 aatggtcctt gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg
    26521 gaatacaggc gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga
    26581 acgccctggc ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat
    26641 gctggatctt ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt
    26701 aggttctttt tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa
    26761 caacggaaca gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca
    26821 gaatgaaaca agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt
    26881 ggctgatgta gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg
    26941 aacagcaatg aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg
    27001 aaaaatgggc gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta
    27061 ttccaaacat ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg
    27121 agcaacaggc cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac
    27181 agatgtagtt ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat
    27241 ctgaagcgat ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt
    27301 agccatggtg taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg
    27361 aagaagtatt taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata
    27421 aagtgacgga tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg
    27481 aattcgtttt agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac
    27541 aaattgaaac agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa
    27601 taaaagcaaa gaaggataaa aagctgcatg cagcttttta tccttctttt tttaatcttt
    27661 ttgacacgta aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa
    27721 gtaaagcggt tttctatata attattaata attcattaca caaagataag aataaccttt
    27781 gttataaaac gatttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg
    27841 aagcagagag gggtattcga ttgtccgata accaattatt agaagttcag aacctacata
    27901 caggattccg actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag
    27961 acaaaaacga gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta
    28021 ctacgattat gggattactt gatcctaata acacaaaaat tacaggtgag attttatata
    28081 acgatctgaa tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata
    28141 tcggaatgat tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc
    28201 agatcaaaga aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg
    28261 cactagaatt gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc
    28321 cccatgaact atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca
    28381 aacctccaat cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac
    28441 agattcttga tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca
    28501 ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac
    28561 agttcgttga agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac
    28621 gctctttact gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg
    28681 aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta
    28741 ttccatggat accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga
    28801 accacttggt acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg
    28861 tgtagatgac agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg
    28921 gattcttcaa tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg
    28981 aaaaaggaaa gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa
    29041 aagccgttgt gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg
    29101 taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc
    29161 aagattcaat gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca
    29221 tccgaaattt tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag
    29281 atatcgttgg gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac
    29341 aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg
    29401 atgaacctgt atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac
    29461 gaatccagca agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa
    29521 aacacatgtg cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa
    29581 gagaagacat ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc
    29641 cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag
    29701 agtatatcca aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta
    29761 cgatcacaac tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac
    29821 gattctgcga cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt
    29881 cttattagcc caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga
    29941 tccggcagtg atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca
    30001 gtacttccgt tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa
    30061 acgctctgtt gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt
    30121 gacagttgtc cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca
    30181 aaattcaatc ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt
    30241 atttatcttc ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac
    30301 aagtggatca caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt
    30361 gaaatatatt ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca
    30421 atatctgcga agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc
    30481 caaaggggta ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc
    30541 gatggcttct caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga
    30601 aaagattttt ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga
    30661 ctataccgtg atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt
    30721 actttcagat atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc
    30781 agaaaggaga atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct
    30841 cctatgggga tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca
    30901 ttaggtttgc tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa
    30961 gacaaagtga tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca
    31021 caaggacaaa agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg
    31081 atcataggtg ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc
    31141 atcggggttg gattagggat catctctggc tactacggag ggatcattga caacatcttg
    31201 atgcggatcg ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta
    31261 tcgattattt ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg
    31321 gtagctaaag cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc
    31381 agtgcctcaa aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg
    31441 aatctaagtt cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc
    31501 gagacaactt tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca
    31561 ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc
    31621 gcatcaatcc ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag
    31681 cgttctgcag atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat
    31741 gaagaaaacg atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg
    31801 gggcggaggt acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga
    31861 attaactttt ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga
    31921 agctgcggtc gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga
    31981 ttcatatgat gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga
    32041 tttcagaatc acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc
    32101 gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga
    32161 tgtgattttc ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga
    32221 cactttccgt aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc
    32281 cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag
    32341 catgcttcaa tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg
    32401 tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg
    32461 cccttattac atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca
    32521 ctactggaat ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag
    32581 tgcaactgaa gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac
    32641 ttacgatacc tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta
    32701 tacttatcta ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa
    32761 tccagattca aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa
    32821 taatgcagta ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc
    32881 tccagtattc cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa
    32941 agcgaaaaaa ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga
    33001 agacaaagat ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc
    33061 gcaaccattg gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata
    33121 cacaacaggt cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc
    33181 tgaaattgat gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact
    33241 atggggacca aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt
    33301 gatcaacgat atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca
    33361 aaaatggcaa caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga
    33421 agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc
    33481 atgggcaaat gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa
    33541 agggcgatgt cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt
    33601 caaggcgctg atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat
    33661 taagtcaaat ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc
    33721 gaaactaaac gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt
    33781 accttttgac cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa
    33841 aaaatatcca taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa
    33901 aactataaca tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc
    33961 tatgtgaatg agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga
    34021 gatgccgttt tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca
    34081 gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt
    34141 gccaaagaat gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga
    34201 ggacgtacgc gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat
    34261 cttgaccagg gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc
    34321 aaagcaggtg ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa
    34381 aaatcaggtg atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa
    34441 cgagtatttt ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa
    34501 tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa
    34561 gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga
    34621 caagacagtc attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg
    34681 aaaaagcaat attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct
    34741 gcgaggcgga aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa
    34801 tattgcagaa gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa
    34861 tgagatcagt gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa
    34921 acaaagctgc agattaaaag atatccaaga attgttctta gattctggat taggaaaaga
    34981 atcgttctca atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga
    35041 ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa
    35101 agcagaacag aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca
    35161 cgaattggaa gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt
    35221 acggctaaaa gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac
    35281 agcaaaaaaa gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg
    35341 caaactatct gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa
    35401 tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa
    35461 actgaagcag acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa
    35521 aagctcgcaa gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga
    35581 aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc
    35641 tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga
    35701 attattggca gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg
    35761 aaacgaactg aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac
    35821 gttagccaag caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga
    35881 attgacacaa aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga
    35941 aatgatccaa caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat
    36001 gtatcaatta atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat
    36061 ccaagaaaac tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca
    36121 gctttcgggt attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt
    36181 agctatcgaa acagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga
    36241 tgctcggcaa gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc
    36301 actgacgaca atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt
    36361 agaagggttt atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt
    36421 ggttcaaaac ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat
    36481 cgcgcaaacg atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc
    36541 cggaggatcc atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca
    36601 aaatcaagaa ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc
    36661 acaagaaaaa aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga
    36721 agttttgcgt acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct
    36781 acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga
    36841 gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga
    36901 caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag
    36961 tcaagaaagc gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga
    37021 acaagcagat cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg
    37081 tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc
    37141 gacattgaca gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat
    37201 taacgaatta tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca
    37261 gcgagataga aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg
    37321 tgagcaaaaa gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga
    37381 aatgatttta gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa
    37441 agcttcccaa gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc
    37501 cttaaaagaa cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga
    37561 acaagtcagt gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa
    37621 aaaccaattg tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga
    37681 agtatttgaa gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg
    37741 taaagctgaa ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga
    37801 agtacaacct cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc
    37861 acttacagcc atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat
    37921 cttagatgag gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt
    37981 gagcgatttc caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt
    38041 cgcagcagat gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc
    38101 tgtacggatg gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg
    38161 aaaatgatca aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg
    38221 atttcagatg agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt
    38281 ctatgtacag gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta
    38341 gatgaaggtg actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt
    38401 gaaatcatgg aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct
    38461 cagcaattgg atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt
    38521 gccccaagga agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg
    38581 gatataagcg atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa
    38641 gaagaattag atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc
    38701 atcaaaacaa gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt
    38761 atttctttat tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat
    38821 gaggaaaatg atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct
    38881 gtgcctttcg tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta
    38941 gccaaagtga tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa
    39001 aatcaagcgt gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta
    39061 cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc
    39121 caattttcga acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc
    39181 agatgtaggt tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact
    39241 cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct
    39301 ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac
    39361 agttattttg tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc
    39421 ccatcagttc aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc
    39481 tggagcaatc gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg
    39541 caatgcagga ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga
    39601 gcaggcagat gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat
    39661 gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga
    39721 aacacttctt gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt
    39781 caaagaaaca acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg
    39841 cggtatcgta cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg
    39901 tgaaggaatc gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa
    39961 aggattacta aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca
    40021 gctccttgtt tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata
    40081 cttatttgta agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat
    40141 tagattcata tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt
    40201 caatgcgcgc ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt
    40261 tgatcttgca ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta
    40321 tcacccctat atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac
    40381 cgattctatt cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc
    40441 tataccgaaa gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga
    40501 cgaagagatc attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt
    40561 agcaaaagaa tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt
    40621 tggcgatgag ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga
    40681 attcaaaaca gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat
    40741 cacttattta ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca
    40801 tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa
    40861 tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga
    40921 agaagggaaa ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa
    40981 gaaagtcgca gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt
    41041 gaatccttat gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct
    41101 gatgtacatg ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac
    41161 gactcgaaat aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga
    41221 aggtagagaa attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc
    41281 tctttattta gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc
    41341 gaaactctat caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca
    41401 tcaatattat aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga
    41461 actttctact cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt
    41521 agatgagtcg gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa
    41581 tgcaaacatg acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt
    41641 aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc
    41701 catggaagaa gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc
    41761 taaatcaacc aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga
    41821 ttatcaagca ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt
    41881 tatgccggga acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt
    41941 gcgagtccct gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga
    42001 aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt
    42061 aggtgaatta gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca
    42121 agccaatcaa attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat
    42181 cgatatgact gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc
    42241 actaggagct aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac
    42301 aactgataaa aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca
    42361 tgtttatcct tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata
    42421 tcctgaagta ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga
    42481 tcaaaagctt tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc
    42541 cagaagcttt ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg
    42601 tatttttttt cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat
    42661 ccgaattcct caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt
    42721 ttcgttgcta gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt
    42781 atggaagcat ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg
    42841 atcatttcag caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta
    42901 tgctgttctt cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt
    42961 acatcatctt gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata
    43021 gcatattccc aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca
    43081 cttaaaataa gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg
    43141 actagtagca ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt
    43201 acaattgtca gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg
    43261 tttgccacga acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat
    43321 catgcaacat ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag
    43381 tgcgcgttta ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg
    43441 atccttgaca gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg
    43501 attgttcctc cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat
    43561 cattggttca gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc
    43621 atggcttcaa gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt
    43681 tgttccattg ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg
    43741 cgaatcgttt gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg
    43801 cttagtatca ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg
    43861 agtgatcatt ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg
    43921 cgtaactgct ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt
    43981 gatttttcct tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt
    44041 cctttatctt ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg
    44101 gtatgcttgt tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa
    44161 actggcattt tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc
    44221 tagctgtcag cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc
    44281 cttcagtatt gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta
    44341 tcggaggagt gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc
    44401 tgatggtcgg gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc
    44461 tttttttatt actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct
    44521 ttttacaagt catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa
    44581 gctttggcaa tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct
    44641 ggtacctgtt atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag
    44701 cagtaaacct cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc
    44761 ttttgacagg gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg
    44821 caaatatcag ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct
    44881 ttctggtagc tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct
    44941 ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat
    45001 tgattcgcga aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca
    45061 tggaaaatca gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt
    45121 tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc
    45181 aaaagataca ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga
    45241 attagccaaa aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt
    45301 gaaagaatta gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga
    45361 cgaggaaatc gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc
    45421 gattgacact ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca
    45481 agacacggat tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt
    45541 tcaaatcatg cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt
    45601 tcttcatgat atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg
    45661 acagttattt acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt
    45721 atacgaaatg gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta
    45781 aaactaacat taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat
    45841 catttggttt agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa
    45901 gcagcacaca aactgaaact acattgacga taacagatag taatggagac caaatcgagg
    45961 tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg
    46021 ctcttggtgt tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt
    46081 catcctataa aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta
    46141 atcagcttca gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc
    46201 taagtcaaat tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt
    46261 tccagcaaaa tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc
    46321 aattagagga attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca
    46381 aaaaagcact agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta
    46441 gattcggttt catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat
    46501 ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat
    46561 ttgtagtaga cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca
    46621 atgaactggt agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag
    46681 atgtctggta tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga
    46741 atcaagcatt taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa
    46801 tgtttcttac attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc
    46861 gtaaaatcgt attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa
    46921 cgtgaattca ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag
    46981 tgtgatccat tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc
    47041 gaaacttaga taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac
    47101 gatgttccat ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta
    47161 ttttctgtcc agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa
    47221 cgatgttcca tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct
    47281 attttctgtc cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa
    47341 acaatgttca atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat
    47401 ttaggcatgt gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc
    47461 ggcgggaagc ttactccacc gttttaataa ttgatagaaa taactctctt ctgctttgat
    47521 tttatactcg cttaatatca atccaaaact attcgttttt tttgaagcta attttttata
    47581 aatatccaat gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga
    47641 accctcgata ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt
    47701 gattcctcca aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat
    47761 ccgtgaacaa aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt
    47821 attttttgtt atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct
    47881 ttttccgttc aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa
    47941 atcataataa acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat
    48001 tagttgcatc tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta
    48061 gaagtgacaa aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt
    48121 ttattagata tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg
    48181 gatacggcat ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat
    48241 tctgtagtgg ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac
    48301 atggatgatc catccacact tgctttagaa ccaacttata atagtttaca attaaaagga
    48361 ccaagcggct tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg
    48421 ggaaaattgg cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag
    48481 ctgatgcagc tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga
    48541 gatacggcta ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga
    48601 ttatctacga catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg
    48661 atggaccatt ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag
    48721 accgttcttt tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac
    48781 gaggcaatcg tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa
    48841 acagatctcg aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg
    48901 gaagcaaaaa aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc
    48961 atgtatcaat ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa
    49021 tcattcggag aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg
    49081 catcgtcgct ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt
    49141 tattattatg aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc
    49201 tatgatccta tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat
    49261 caaacacaac atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac
    49321 agcattagta atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta
    49381 gatccattta tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt
    49441 gatcggatga tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg
    49501 ataatcacaa aaaaaatata atatacattt tttatttaat tataattaat attgattgaa
    49561 gtctaggcag tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag
    49621 gctatgacag ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc
    49681 aaatcgggct gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt
    49741 tcttttattt cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga
    49801 tatgtttagt aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag
    49861 cagcaataat gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat
    49921 acccaaagga atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa
    49981 ctcctcctgt tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc
    50041 caagccctgg aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga
    50101 ctcaaaaaat ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg
    50161 ggtctagaaa accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga
    50221 aattaagtgt ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag
    50281 cacagctgaa atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc
    50341 aaacggtgaa ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg
    50401 ctaaatcagg aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa
    50461 atcaagatgc ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt
    50521 ccactaaatt agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact
    50581 gttaaaaata aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag
    50641 gctaggctaa atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa
    50701 aagatatatt gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata
    50761 tctttccgat catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag
    50821 acgaagaaag caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag
    50881 aaagcagtga ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg
    50941 agggttcgga caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg
    51001 atcccaatat tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca
    51061 tattggaaaa tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta
    51121 tttggtcaaa taagaagatt agtttactct cggattttac atttaaatct tatttatact
    51181 taggaaatca acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta
    51241 gaatggctag taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt
    51301 ctactcgttc tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata
    51361 aaaatagtgg ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg
    51421 gacatgtcgc ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg
    51481 gcgtgacggt agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat
    51541 gggacgcgga gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga
    51601 ttatacctga tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt
    51661 caacaggtac tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca
    51721 catcaacagc tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag
    51781 gaaacgataa gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata
    51841 agaaaatgca gactagtatc gacctcccac aagaattaac tattcccgag catatcttga
    51901 tcaatggaaa tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa
    51961 atctgacaga ttattttatt gctggagata cgttaataat cgaattggag acaactttgc
    52021 aggaagtaat tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt
    52081 tgattgagca atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc
    52141 atgtttatta taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg
    52201 ggaaaatagg agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact
    52261 cggattcagc gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt
    52321 acagaaaagg caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata
    52381 aaatatccag tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag
    52441 atgaacgggc ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg
    52501 gagattttga gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag
    52561 ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc
    52621 ttttagattc tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa
    52681 tcggaacctt tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg
    52741 aagagaagaa tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt
    52801 gtgtcccaca gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat
    52861 tttcttgaga ccaaaacaac aattatttat aacactccta gttattttag gggcactttt
    52921 taacaatata tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga
    52981 gaatcaaatc gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt
    53041 agaagtaaaa gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt
    53101 gaatagcgca accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa
    53161 agacatgagc ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct
    53221 aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga
    53281 tggtgtattg gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga
    53341 aactaaggat caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat
    53401 gcaacagaca ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca
    53461 aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa
    53521 tcaagtagtt attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga
    53581 ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa
    53641 tggtcaaccg ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa
    53701 tgaaaatgga aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg
    53761 ggtatttgaa aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga
    53821 tgttacgatt aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt
    53881 gtttatttta ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta
    53941 aggttaagat aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa
    54001 caatataaaa tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa
    54061 taacaggttt gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga
    54121 caggacgatg tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa
    54181 tcagttttat ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg
    54241 atgactaatg aattatgagt tttttttaag caaagaagat cacagaagtt attcattact
    54301 taaatattta gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt
    54361 gtcgctatcg acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa
    54421 gttcgatcta gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat
    54481 taatggaaaa tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat
    54541 gaaaatggta ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca
    54601 gcatcacgtg agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa
    54661 aaaatatcaa attttttttg aaaagagaaa gttga
//
LOCUS       DAPBCS010000013        54476 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPBCS010000013 DAPBCS010000000
VERSION     DAPBCS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.013"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(71..1492)
                     /locus_tag="RD521_000959"
     CDS             complement(71..1492)
                     /locus_tag="RD521_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7770709.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="RD521_000960"
     CDS             complement(1735..3612)
                     /gene="tdc"
                     /locus_tag="RD521_000960"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDT7770710.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3903..5159)
                     /locus_tag="RD521_000961"
     CDS             complement(3903..5159)
                     /locus_tag="RD521_000961"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDT7770711.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(5271..5476)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5739..7040
                     /locus_tag="RD521_000962"
     CDS             5739..7040
                     /locus_tag="RD521_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDT7770712.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7109..7708)
                     /locus_tag="RD521_000963"
     CDS             complement(7109..7708)
                     /locus_tag="RD521_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7770713.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7962..8843
                     /locus_tag="RD521_000964"
     CDS             7962..8843
                     /locus_tag="RD521_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770714.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8856..10466
                     /locus_tag="RD521_000965"
     CDS             8856..10466
                     /locus_tag="RD521_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDT7770715.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10543..11202)
                     /locus_tag="RD521_000966"
     CDS             complement(10543..11202)
                     /locus_tag="RD521_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7770716.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11485..12150)
                     /locus_tag="RD521_000967"
     CDS             complement(11485..12150)
                     /locus_tag="RD521_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDT7770717.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="RD521_000968"
     CDS             complement(12297..12902)
                     /gene="dhaL"
                     /locus_tag="RD521_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDT7770718.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12930..13940)
                     /gene="dhaK"
                     /locus_tag="RD521_000969"
     CDS             complement(12930..13940)
                     /gene="dhaK"
                     /locus_tag="RD521_000969"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDT7770719.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(13915..14361)
                     /gene="dhaM"
                     /locus_tag="RD521_000970"
     CDS             complement(13915..14361)
                     /gene="dhaM"
                     /locus_tag="RD521_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDT7770720.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14464..14784
                     /locus_tag="RD521_000971"
     CDS             14464..14784
                     /locus_tag="RD521_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDT7770721.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14896..15765)
                     /locus_tag="RD521_000972"
     CDS             complement(14896..15765)
                     /locus_tag="RD521_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7770722.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15861..17165)
                     /locus_tag="RD521_000973"
     CDS             complement(15861..17165)
                     /locus_tag="RD521_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7770723.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVSFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17162..17671)
                     /locus_tag="RD521_000974"
     CDS             complement(17162..17671)
                     /locus_tag="RD521_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770724.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17846..19981)
                     /locus_tag="RD521_000975"
     CDS             complement(17846..19981)
                     /locus_tag="RD521_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7770725.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20064..21719)
                     /locus_tag="RD521_000976"
     CDS             complement(20064..21719)
                     /locus_tag="RD521_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7770726.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21721..22035)
                     /locus_tag="RD521_000977"
     CDS             complement(21721..22035)
                     /locus_tag="RD521_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7770727.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22205..22465
                     /locus_tag="RD521_000978"
     CDS             22205..22465
                     /locus_tag="RD521_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDT7770728.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22479..22793
                     /locus_tag="RD521_000979"
     CDS             22479..22793
                     /locus_tag="RD521_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDT7770729.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(23523..24077)
                     /locus_tag="RD521_000980"
     CDS             complement(23523..24077)
                     /locus_tag="RD521_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDT7770730.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(24203..26132)
                     /locus_tag="RD521_000981"
                     /pseudo
     CDS             complement(24203..26132)
                     /locus_tag="RD521_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(26438..27154)
                     /locus_tag="RD521_000982"
     CDS             complement(26438..27154)
                     /locus_tag="RD521_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDT7770731.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="RD521_000983"
     CDS             complement(27155..28978)
                     /gene="glpO"
                     /locus_tag="RD521_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDT7770732.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="RD521_000984"
     CDS             complement(28992..30488)
                     /gene="glpK"
                     /locus_tag="RD521_000984"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDT7770733.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(30624..32084)
                     /locus_tag="RD521_000985"
     CDS             complement(30624..32084)
                     /locus_tag="RD521_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770734.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(32074..33369)
                     /locus_tag="RD521_000986"
     CDS             complement(32074..33369)
                     /locus_tag="RD521_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7770735.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(33701..34378)
                     /locus_tag="RD521_000987"
     CDS             complement(33701..34378)
                     /locus_tag="RD521_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDT7770736.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(34408..35499)
                     /locus_tag="RD521_000988"
     CDS             complement(34408..35499)
                     /locus_tag="RD521_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDT7770737.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(35600..36034)
                     /locus_tag="RD521_000989"
     CDS             complement(35600..36034)
                     /locus_tag="RD521_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7770738.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(36618..38075)
                     /gene="ascB"
                     /locus_tag="RD521_000990"
     CDS             complement(36618..38075)
                     /gene="ascB"
                     /locus_tag="RD521_000990"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7770739.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(38072..39967)
                     /locus_tag="RD521_000991"
     CDS             complement(38072..39967)
                     /locus_tag="RD521_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7770740.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(40025..41800)
                     /locus_tag="RD521_000992"
     CDS             complement(40025..41800)
                     /locus_tag="RD521_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDT7770741.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(41850..43346)
                     /locus_tag="RD521_000993"
     CDS             complement(41850..43346)
                     /locus_tag="RD521_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7770742.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(43540..45162)
                     /locus_tag="RD521_000994"
     CDS             complement(43540..45162)
                     /locus_tag="RD521_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7770743.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(45490..46803)
                     /locus_tag="RD521_000995"
     CDS             complement(45490..46803)
                     /locus_tag="RD521_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDT7770744.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(47048..47272)
                     /locus_tag="RD521_000996"
     CDS             complement(47048..47272)
                     /locus_tag="RD521_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770745.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(47292..47714)
                     /locus_tag="RD521_000997"
     CDS             complement(47292..47714)
                     /locus_tag="RD521_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDT7770746.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(47856..48566)
                     /locus_tag="RD521_000998"
     CDS             complement(47856..48566)
                     /locus_tag="RD521_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770747.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(48584..49081)
                     /locus_tag="RD521_000999"
     CDS             complement(48584..49081)
                     /locus_tag="RD521_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7770748.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(49118..49570)
                     /locus_tag="RD521_001000"
     CDS             complement(49118..49570)
                     /locus_tag="RD521_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7770749.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(49705..50310)
                     /locus_tag="RD521_001001"
     CDS             complement(49705..50310)
                     /locus_tag="RD521_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7770750.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(50760..51380)
                     /locus_tag="RD521_001002"
     CDS             complement(50760..51380)
                     /locus_tag="RD521_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7770751.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(51382..52251)
                     /locus_tag="RD521_001003"
     CDS             complement(51382..52251)
                     /locus_tag="RD521_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDT7770752.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(52274..52681)
                     /locus_tag="RD521_001004"
     CDS             complement(52274..52681)
                     /locus_tag="RD521_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDT7770753.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(52700..53989)
                     /locus_tag="RD521_001005"
     CDS             complement(52700..53989)
                     /locus_tag="RD521_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7770754.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            54047..>54476
                     /locus_tag="RD521_001006"
     CDS             54047..>54476
                     /locus_tag="RD521_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7770755.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT        15484 a        11504 c         9364 g        18124 t
ORIGIN      
        1 aaaaaactat tcacttggca ggacctccta ctaaatcagc gacaaaacgt cggctgaaag
       61 acttattttt ttatttttct tctaaacttt tttctacctc aatcccccaa gaatctttct
      121 tgaagtgatt gatgatcaat ggaataacga acatgaccac tgtgatagct gctactaata
      181 atgcatcact caatgtcgct gtacctaatg tagcaaatac gacaacgatg atagagaaga
      241 tcagcatgca gctgactacc caagctaatc cgtttccgtt tttaccaatt cggtaaggac
      301 gttcgaattc tggatgtttc ttacgcattt taagcaatga aacagcaatc aaaatgtaca
      361 cgatacaata aataacagtt gtagcatttg taagtgtcaa gaatacagca cttacgtcat
      421 ccattacacc ataaagcaaa gcaaataatg aaataacgat tgattgtgtt agaacgacat
      481 ttctagatac gccgtatttg ttttctttat ggaatccgaa ttttggtgga aggaatcctt
      541 cacgagctac ttggatgatt gtttttgatg gtccagtaac ccaagctgat aattgtaaaa
      601 gcacaccaat aaataccatg aagctgaaga tatttccgat cacttctggt aagcctagga
      661 tttgacagtc aatcaagata ggctgcgtga tatttgctaa ttccattttt ccatctggta
      721 cgacatttgc tacaagcatc gcattgatga cattcaataa aactaatccg atcaaagcga
      781 taaatacgcc ttttgtataa ttttttgttg cgtctttcaa acgaggcatg taaacagaac
      841 tcatttcgat cccaacaaaa ataaatgtga taccagctaa gtatttcaat gaatcaatat
      901 gttccaagtc agggaaagct tttgaccatg aaaagtcacc tagatagccg tttgagacta
      961 gtccgacttt gaaggctgcc aaaacaccta aaacgaacat gatgactact gggatatata
     1021 cccctagcca tacaccgatg ttcccgccga cttttgccat atcgaatttc aagttcaaga
     1081 tcgtgatgat ccagtagata actaaaatac aacctaaaac gaaccagtga ttattgccaa
     1141 gttctacatt accaaatgtg tttcctaaca atggtcctaa agtagaagcg accattacca
     1201 tacctgggaa catttgcacc cataatagcc atgcaacgac aaatccccat ttgctgccca
     1261 gtgctgtttt tacccataac tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac
     1321 cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca
     1381 atgtccaacc gactgcggct aacgtaggaa tactccgaac cgttgcacac agtgccattg
     1441 tcagaccgat aaaggtccca agacccattt tacttacttc agatttactc accgttgaca
     1501 ctctccttga ttttttggat aaaataacgg tgtctttgaa gtagtttcag caataaacga
     1561 aacaattcag cgaatatttg aggggattct ttgagtttat tgcttgaagg gtttcttcaa
     1621 agacaacctc aaaacaaagg gatcgaagag cttaggtctc cccttgctct tcagatccaa
     1681 acatgcgtca gaaacagtta gtattcaagc tgttttctaa catgattacc ttgcttattt
     1741 tgcttcgctt gccaataatt ggtctgcgta gattttttcc aatttttctt gaagagctgc
     1801 tttgattttt tcagcatatt cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac
     1861 gctggcacgc aatacagtta cttttccggc acggttccat tcttcatctg agaatcctag
     1921 ttggttcaca aattgtaatg ggctgttgcc atagtctggg atagcaaagt cagtatgtga
     1981 agttaagaac tcattaccgt aaatgcttcc tttcacgtat gaagaataat catatacgtc
     2041 atggttcaat ttattcattg caactaagtc atcgttgcct ttttctttga atacatagtc
     2101 taccatattg aaatctggat aagttaatgg atgaacttcg atttctttgt ctcctacttt
     2161 aaatgacaag tcattcaaga agttgtagaa acgatgtgca ccttcgattg atgcacccat
     2221 taatttacca tatcctgtga cattcaaagg taagacatgg tgtgctgccc aaacgctggc
     2281 tgctgttgcc cctgcttttg aaccttccaa gatataagcc ccaagtaatg ctgggatatc
     2341 tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata acgtctctca tgcggatatc
     2401 tttgatgacg ataccaccgg cagaatatgg aacatagccc attttatgtg ggtcaatcgt
     2461 tacagattct gcttcttcga ttgctttata tgcactgtgt acttcttcta agatataatc
     2521 tttgttttct gtaaagacat tgtatttgta atgaacatct tttaaatctt caaatggaat
     2581 gaagttgttg tcttcgtcta agaaaatcgc acggccgtat ccgccataag cagcatctac
     2641 gtgtaagtag aagtaaatac cgtctttttc taatacgcga cgcaatgcaa cgattttatc
     2701 gataccgtcg attgctcctt cttctgttga tcctacaaca ccgactacac ctaggattgg
     2761 tgttttttca gctgctagac cacgaacgat tttttcaagt tcgttgatgt ccatgcgata
     2821 gttgtggtca actggcactg gaatcacttg gtctaaaccg ataccgatga tgtcggcagc
     2881 ttttagccat gaatagtgtt tagtttgtgg aaccaaccat tttcctaatt tttctaaatg
     2941 ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt ttttctggga cgctgtctaa
     3001 cagattcatg atttcttctg ttgacaagtt cattaattcc caatcgcttt ttccagcaac
     3061 tagttcaggt gtcacttctt tcattgcaag cggcaatgat ttgatgttgc gggcatacca
     3121 aagtccttct aagtttgcta aagaaccatc agcaacgatg tggccccagc catctttata
     3181 gctcattaat tttgcaaatt ccattcctac ttcttcttcc atttggcttg ttgcaggaga
     3241 agattcatag gcaacgttat taccattcca tagcatagca aaattgtacg ctagtaaaga
     3301 tggcatcaat gtttcagagt tcatatgacc ccagtaacgt ccggcattat gccaaggaac
     3361 ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca tcctttgttt tattcacagt
     3421 gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata ataggcatgt cttgaggcat
     3481 gtagttttga cgccagccta aatgttcatc caccaattca ttcaacaaag ctttatagat
     3541 ttggccgttc tcagctttgt ccccaatgaa tagggcattt aggtttagat ctttcgacaa
     3601 tgattcactc atagtgaaac cctcctaaaa aaatatttaa attttgtcga gccctacatt
     3661 acacctgttc ttctattctg taaactaagt tttggcctga tttttaatca tattttaata
     3721 tgtatcaatt atccgaattg ttcacacttt tgaaataatt aaaaaaaacg agggaattta
     3781 caaatgtaaa ctccctcgca aagattgtat taacgacaac cagcgttcta ttttaggatg
     3841 ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt caaggtaaaa acttactaaa
     3901 ttctaatcaa ttactttggc caaaaagtaa tttttcttac cttttcgaat aaccacaaac
     3961 ttaccatcga attcatcgga tggatttatg acaaaattta aatcggtaac gcggtcaccg
     4021 ttaatactta tagctccgtt tgaaacatct tcacgtgctt gacgtttaga tggttcaatt
     4081 tttgtggaaa caagcagttc cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc
     4141 attttaccga atccttgcgc gatttcttcc gcatttagtt ctttaatatt tccagagaac
     4201 aatgcttgcg ttattttttg cgcttctttc aagtcttctt cactatgcac aaatcttgta
     4261 acttcttctg ccaaacggcg ttgtgcttca cgtttctcag gttctgtctc tacttttttc
     4321 gctaagtctt cgatttcttc ttgagacaag aaagtaaaga atttcaagta tttcactaca
     4381 tcgcgatcat cttgattcaa ccagaattgg tagaactcaa atggtgaagt tttcttcgga
     4441 tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc
     4501 aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt ctaattttcg gattaggtct
     4561 aatccagctg tgatatttcc ccattggtct gctccaccga tttgcaattg cacatcgtat
     4621 gttttatgca gatgcaaaaa gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat
     4681 gagattccta cttccaaacg gctggcaacg atatcttttg ctaacatcgt gttgatgttg
     4741 aagtttttac cgtagtctct caaaaattca agtaaagaga tttttgacaa ccagtcgtag
     4801 ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt ttctcatttg attggaaagt
     4861 gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca cacgttctgt tttgcggccg
     4921 cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa tatatggatg atggcctgcc
     4981 aattggaatc ttttcatcat cataaatgga atcaaatgtc cgatatgcat cgaatcacca
     5041 gttggatcaa cgccgcaata aagtgaaatg ctcttttctt caacgagctc gcgaagtctt
     5101 tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta attcatcaat aatattcatt
     5161 ttactgacct ccgtgtagtt tttttatgaa agcaggaacc tgctagcata tgtaattaat
     5221 tatttcttca ggataggata ggaaataaaa aaagtccctt ggcatataag ccaagggacg
     5281 aaatctcgcg gtaccaccca atttgtatgt ctagatatcc aacatacctc tcgaacattt
     5341 aacgctgtcg cgtggcaatc actgccaaag actcctgaaa tgtaattcgc tttaaagcat
     5401 gtactggttt gcagcgacca ccagctttct gaaacattgg ctttaacaac tacttcgttt
     5461 cgatcttcgt cttgttatta ttaatatgtg tctatcatag agcgcaactt tttctttgtc
     5521 aacagaaaaa ggatattttt tttaaattcc tcattttttc ttttttgaat ccgctctaat
     5581 tccgaacatt ttattttcaa ttattgttaa aagaaataac tttttctgta ttttttctgc
     5641 aatttatcga gaagtcactt tactttgcct cttattcttt cttcgttaca ataaggaatg
     5701 ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta
     5761 ttttaggagc cacttctgct ttaactatcg ttattttaat tctaagtttg ttttttgtca
     5821 tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa
     5881 aagaaccgac agatgataag caacaactag ttactcaagc aaaaaatctg gctcaaagct
     5941 acgattatga ccaagcgatt gcgttattgg aaaaagatag taagaacgga tctactgaaa
     6001 cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca actgatcgac tggtctgatc
     6061 ctactcagat ctctcatgtc tttttccaca gcttgatcgt ggacccgcaa aaagccttcc
     6121 actctcaaca ggcacaagga tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt
     6181 cgatcgaaca actttatcaa aatgattttg ttttagttaa tttgaatgat ttgatccaaa
     6241 aagacgaaca aggaaatctt tcctttaacg ggcttgcgct acctgaagga aaaaaaccat
     6301 tggttctttc acaagatgat gtcagctatt atgaatatat ggataattcc ggttttccta
     6361 gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt ctataaagag aaagataaag
     6421 aagaaatcgg tgactttgac atggttcctc tgatcgatgc ctttgtcaag aaacatcctg
     6481 atttttctta tcgaggcgca aaaggtacct tggccctgac aggttacaac ggagttttag
     6541 gttatcgaac atcaaaatct caatatggag acaatgaaaa aacaaaaaaa gagattaaag
     6601 aagctaaaaa agtggcggaa cagttgaaga aagacggctg gagctttgct tcccatacat
     6661 ggggtcactt gaatatgact caagcttcac tagaagatgt aaaaaaagac aatgaattgt
     6721 ggcagcaaga agttgcacct atcctaggaa aaacaaatat tctgatttat ccatttggtg
     6781 cagacatcag cgactggcag ccttattcag cagaaaatca aaaatttgct tacttgaaga
     6841 gtcaaggttt cgatattttc tgtaatgtcg atgcttctac accagcttgg ggacaattag
     6901 gtacggattt ctatcgaaat gctcggatca atatcgatgg cattcgtttt caatctgatt
     6961 tagacggaaa aaatcctatt cttgatccat tcatcaatgt ccatgatgtc tatgacaaag
     7021 aagcacgcga atctgcttga ttttacgtca tttatgaacc aaaaaagccg aactgacatt
     7081 ttatcgtcag tttcggcttt tttatgcttt atttcttctt cggtgcgatt cccttgatca
     7141 ggacttctgt ccattctgct gtatatggaa gcagttttgc gtatatctcc ggcttagaaa
     7201 ttccttctac cattgcttga aaatagagga tcaagaactc atccgaatat ttcaaatcca
     7261 cttttccttc tttccgccct cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg
     7321 tatacgcatc catcgcgtca gaaaattctt ttttttcttt ttttgtatag tatgtcatca
     7381 aatcgatata aaattgtttg ttcactcgat tgagataacg gattttgttt tcactcatgg
     7441 caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta
     7501 aatcattcat aaatgcaaaa aatacttcat aaatcagccc ttccttgctg ccaaaatatt
     7561 taaaaatcgt cgctttactt acctgagcgt tctcagctac cttatccatt gtcactccag
     7621 cggttcctat gtctgtgttc atcaagtcaa aagctgcttg taaaatctct ttctttttct
     7681 cttgtgctct cttttcaaaa ccgttcattt gtagagcctc ccgtaatttt aaacttttat
     7741 aatatatata gttcaaattt attctgaggt ttattataac tatctattga gaaagtatca
     7801 agctagcaat tccttgttta tgaattcatt gacagaaatt tataaaaaga atattatgta
     7861 ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc tttttattaa actatttaat
     7921 cttgtttagt atatattttt agaaaaagga gtggggtttt tatggcagaa atcgtcaaag
     7981 tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg
     8041 tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa tccacgacga
     8101 ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt tttgggcaag
     8161 atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca ggcgatatct
     8221 ctttgtgggg aaatttgact ggaggagcga tcatcgatct cttcatgaag cttcatggaa
     8281 aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat cctaagaaaa
     8341 aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct gctttggctg
     8401 ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct ttgatggaaa
     8461 cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc ctactttctt
     8521 ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata cgcgaaggaa
     8581 cactcgatga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg accatcacta
     8641 tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat gactttgtgc
     8701 aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg attttagctg
     8761 aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt gaggatttat
     8821 tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt tcacgctggt
     8881 ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata tggctagtcg
     8941 gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact tccggacaag
     9001 gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg gtcggaccaa
     9061 ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat gaaatgttgc
     9121 ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc caaacgagaa
     9181 aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa
     9241 actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca atatcgatca
     9301 gctgtacgat gatttttttc caagcggatt cgatcactgc agaaggtgct tggctttttg
     9361 ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt gctgcacaac
     9421 tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt ggactttacc
     9481 ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat cctttaggct
     9541 ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt tatttgtttt
     9601 tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga gatttgaaca
     9661 ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt ttatccttgc
     9721 gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc acttttggtt
     9781 tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta gaaagtaacg
     9841 acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac tttacaagca
     9901 cgatcatggt agtcttgatt tgtctagctg ctattttacc aattgctagc atcaatcgtt
     9961 tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa atttctagga
    10021 accaacttta ttggaccaat atgttactag ctattctatt tgctatattg gcaatcctga
    10081 ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg aaagacatgg
    10141 atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta gtgtttatcg
    10201 gaattagtgg ctttctattc ggatggcttc ctcgaatcgg taaacttgct tatatctact
    10261 tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca gaatggtttt
    10321 ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt gatctttccg
    10381 tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct attggttata
    10441 ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg ctagcctcca
    10501 aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt cttctaactg
    10561 ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat tctcctctaa
    10621 taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag caagttcttt
    10681 cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt caagcagata
    10741 gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct gctgtacttt
    10801 tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat aatgcagtaa
    10861 gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc
    10921 aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc cgcccggctc
    10981 tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt tcatactgcc
    11041 ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt ctcctttttc
    11101 cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt
    11161 gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc ctccttctaa
    11221 acagctattt ctaataaaga aacataactt cccttattcc aactgataat tttcttttat
    11281 catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc tttttgtccc
    11341 ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat tttttaaata
    11401 tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta aggtttgttt
    11461 ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct tttgatatag
    11521 tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga tttcttgcgg
    11581 atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt tcaactcttc
    11641 ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa gttcatgttc
    11701 tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact ctttcagtat
    11761 cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt tataatgttg
    11821 gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac aggcattctc
    11881 gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt
    11941 cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt
    12001 ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct tgatttgttc
    12061 aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa
    12121 caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta tcttttgtat
    12181 tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt ctgcatcaag
    12241 tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta ttgaacttaa
    12301 ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc agctcctggg
    12361 tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt agctaccaag
    12421 actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc tgccttcaat
    12481 gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc tgcctttcct
    12541 cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc ttgagaatca
    12601 atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc gtataacgga
    12661 ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa tgtatcgctg
    12721 attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc tcttcccatg
    12781 ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata ttgtttgttc
    12841 tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac ggttagtttc
    12901 atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac attgctttct
    12961 aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag accttgcata
    13021 tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc tctttcttcc
    13081 aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt cccgcccatt
    13141 ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa aatcttagta
    13201 acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata gcctggctca
    13261 ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc tggtttccct
    13321 acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc
    13381 ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg
    13441 attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata cgtactattt
    13501 tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc catagcagct
    13561 aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac gatcagcaaa
    13621 actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg agttggagaa
    13681 gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa cccggcatgg
    13741 gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc tttctgattc
    13801 ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct ttttactaat
    13861 ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt caatttattc
    13921 ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat catctgttat
    13981 accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca ccagatgata
    14041 acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca ataccgcgct
    14101 accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga ctgcttctac
    14161 gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa aaatttgcac
    14221 ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta ctttttgact
    14281 atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta catagttgct
    14341 aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt tctaattttg
    14401 ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga gaggagtgat
    14461 cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc ttataaaggg
    14521 atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga ggcggggttg
    14581 cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa
    14641 gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc gatcattggc
    14701 gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt cacatgtagt
    14761 ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta aagagagatg
    14821 ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt actctacctt
    14881 cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg cgactaacac
    14941 acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt ttggcaaagt
    15001 ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga
    15061 ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga aactagctgc
    15121 taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc
    15181 gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct ttccagaagc
    15241 tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga tcttcccgtc
    15301 aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg
    15361 tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga ttgctgcttg
    15421 gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt ttcttttatt
    15481 ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta ttgatgtatc
    15541 ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag tcatttctgt
    15601 ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt ctgtttgagc
    15661 aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg aaacagcaat
    15721 tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta ctacttttta
    15781 actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac aggctgtttg
    15841 tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca tgtcccgctt
    15901 ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc
    15961 actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt
    16021 cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag
    16081 atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc ttgatataca
    16141 taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat tgaccagaca
    16201 atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa aggatggagc
    16261 atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca acgttttctg
    16321 tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat ctcaggagca
    16381 aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc ttctgtcgct
    16441 cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat cgtatttgct
    16501 ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat gatgcccacg
    16561 atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt ttggacatcc
    16621 atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata attcacatac
    16681 ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat caatttccct
    16741 tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt ttcgatccga
    16801 accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg tgtttggtcc
    16861 gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt cgtcatcaaa
    16921 tactcgattg tatattcgat cacaatttct tcattatggg ctggcaccat gatcgtgatg
    16981 aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt gtataaaaac
    17041 acataacacc agacaccaat aaacgataca aatccgccta aaatcggata agaaacgaga
    17101 aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg ggtgattgag
    17161 atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc gtatctagat
    17221 ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt gttccaaaac
    17281 gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca tcttcatatt
    17341 caaaagattt atttactttt ttgtatagat ttcgattgtt ccacctagtc aatacaaatg
    17401 aggtaatcag gaaactgaaa aaaatcaata caaaaaagcc gattaagaaa tacagcattt
    17461 tcttttctga taaaaaagcg tacaaggaaa aatccttggc tctttctggt ctcgttaatg
    17521 ggaaaaagac ccatataaaa ggaagaacaa cgatcatcca tcctaaaaga gccactaacg
    17581 tttgccaaat ctttaaccag taattacctt ttgaaaaata cttatctttc accaaaattg
    17641 atgcaacttc ttctcttgtt acttttttca tatcctcacc tcacgttttt ttgttacgac
    17701 gtaggagttt accatacatt tcatttcttt tcacataaag tgttcctaaa tttttcctaa
    17761 gaaaaagaac aaaacatttc tcatcagcaa atgaaaaata ttttgttctt tttatcagtt
    17821 gttcttttta ttttttatct cttaattatg ttgctctttc caatacgaca atgcttttca
    17881 ttcctctaga tatcgccgtg tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt
    17941 caaatggata taagattaca tgatcaaatt ccagtccttt tgctagactc aaaggaatga
    18001 tttggcagtt ttctaatcca tatgaatttt ttttaatcga ttcttgtagc gtttgggttt
    18061 cctcttctgt ttgggtgata actgccgctt tttctccttt tagagaaacc aaaatctcag
    18121 ctagtatttt caacaattct tcagaggatc gaaaagagcg gtattcgggt tcttttcctt
    18181 gcggtcggat cgatacgatg tcgatcttct cttgatccac agcataggtt ttgaaaagct
    18241 ctgtgattgc tccgctagaa cggtaactat ttctaagatc ataccgatgg atcggcagat
    18301 ggtagtcgtc gaaacaacgc ataatatcag caaaagtagt ggaagaatta aagattgcct
    18361 gattctcgtc acctactaaa gtaaagtgag tttttggaaa taattcgatc aatagaccta
    18421 attgtgccag ggtataatct tgtacttcat caatcaatag atagcgaaat gctggtacat
    18481 ttaccttttc aacaagtaaa tgacgaatag ctaatataat gactgcttca tcaacagtac
    18541 gtgtgggttg ccaagcataa gctgagccat atcgtttttc ataaattctt tcaaacaact
    18601 gatgctcatc cacccatgcc atttcttcga tttgtctggt tatttttcga tatttttttt
    18661 gtaaaagttt ccttgcataa gctacaatac tttgttccga atcatcagaa atcaatttac
    18721 cgaataattc caactgccta tcttctgtca acgagagtac ctgattctgc acagcaggac
    18781 tttttgcttg tttcaaaaga tgattcttcc attcgctcat cagtgccttt ttcgttgctt
    18841 gaagcctatc gatcaattta ggatgagcag gaactttctc atatattttt tcgatcgttt
    18901 ttttagaaag gactgttttt ccatttttag taagtccacg gataaaattc ggatgatcta
    18961 aaaacaaagg atctgattgt tttagattat cgataaactt tttgctacgc aaacgttctg
    19021 tctgctccga tacattttct cccgtgatac gtttgaaata agcctcttca ccttctattt
    19081 cctctgctga aagctgtgaa accagctgta tgatggttag attcagtgga tttctttcac
    19141 ctaatgatgg taggacattc gaaatatagt cgataaaccg atgattaggt gatagaatca
    19201 gcaagtcatc ggatgtcatc gtatctcgat ataaatagag cagatacgca atacgctgca
    19261 tgatagcaga ggttttacca cttcctgcta cgccatttac caagatattt tgacttttca
    19321 catcccgaat gattgtgttt tgttccgatt ggatcgtagc agtgatagcc ttcatttcat
    19381 tggtctcatc ttggcctaag acttctaata atacgtcatc ttgaatagct acagacgtat
    19441 caaaaaactt gaccaaacga tctctttcca aaagaaattg tcgtctttct ttaataaccg
    19501 tatcgatttt attttgacga accgtataag aagatggacc aatcatgtta ttgtaaaaaa
    19561 gttcggcaat tggtgaacgc caatcataaa caagattttc atctgcatca tctgtgaaat
    19621 ttgctgtacc gatataaaag gattcttttg gctcattatc cagaaagtct acaacgactt
    19681 ttccaaagta gggacttttc aataaacgtt cgatctttgc tagctgattt tctgcgtttt
    19741 tctgtttgat attcatctga tcgatttctc tatttttcat ttcaatcatc gaaaaagtat
    19801 ccaggttatc taagtaactg tcaaaattca atcgaagctc gccagccatt tctttcaacg
    19861 actggtttcc ttctattttt gccgtatcca tcgattcccg cagttcgttt tttttatcaa
    19921 ttagcttttg atacacgttc ttcagatatg cttcttctaa ctgtttttgt gtgttgacca
    19981 tacacattct cctctttttt taaagtggtg gaaccgatta gtttacctct tttaagagtc
    20041 gaatgcaagc tttttaacga gtatcatttg ttaggatata tcagttcttc aggtcggaca
    20101 gtttgatata aagaaaatgc accatcaagg ttttttacgt ggtagccaga ctgcttcaag
    20161 attctttccg ctagatagct acgtaatccg ctatgacagc tgacgatata ttcttgttgg
    20221 ctgtctaatt catttaatcg gtcccgaagt tcattcaaag ggatatgttt ggcttccttg
    20281 aatttgccct tttccaattc ttctgctgtt cgcacatcta ataaaatttt tccttttgct
    20341 agttctgtcg gcaattcatg ccattgtacg gtttcactaa gcccttctgc aatgttcatt
    20401 gctgcatatc ctagcatgtt tactggatct ttggctgagc caaatggcgg tgcatacgtc
    20461 aattctaatt ccggtaaatc gaagatcgtc aaatgccctt tgattgctgt tgctaaaata
    20521 tcgatccgtt tatccacacc ttttgcccca acaccttgtg caccataaat ctcgccagtc
    20581 tctggatgga agatcaactt cagtaaaata tctgttgctt ctggataata gcttgcatga
    20641 tcttttcctg atacatgaat gacttcaaaa gacagttcgt tttctttggc tgttctttca
    20701 cttaaaccgg tggatgcggc agtcattcca aacgttcgta taattgctgt tccaataccg
    20761 ccttgatttc ttcgagaaat tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg
    20821 gctggtgaag caagagaaat aagtgcatct tttccagtga tttgctgttt tacaacgata
    20881 gcatccccca ccgcataaat atcaggaata ttcgtttgat aacgttcgtc tactaggata
    20941 cccccgcgta tgcctaattc gattccagcg gtcactgcta gctggttatc aggacgaaca
    21001 ccaatggata aaataactaa atcagcaggg atggtacgcc catcatccag tcgcaatcgt
    21061 ttcccgtggt cttcaaatcc agcgacagca tgagaagtaa tcacttctac tccttgatga
    21121 gacaattctt tttctataaa agctgccatt tcttcatcca gtgttggaag aatatgtggt
    21181 gcttgttcga tcaccatgac ttctaaacct cttcttttta ggttttctgc catttccaat
    21241 ccgatgaatc ctgcaccaac gatcacggca cgctttggct gtttttctaa tgcatgcatc
    21301 actttatcga tatctggtac atttctaata gaaaaaacat tgtcggcttc tgttatccct
    21361 ggaatcgatg gaacaactgg ttttgcacca ggagataaaa ccaatgcatc gtactgttca
    21421 atgtgttttt gaccctcatg tacgacttct accgttttgt tttcgggaaa gatggctgtc
    21481 acttcatgat ttggacgcac atctaaacaa aaacgagcgg ataatgattc tggcgtttgg
    21541 acaagaagat tttcccgttc actgatttct cccgaaagat aataaggcaa gccgcaattt
    21601 gcaaaagaaa catacggtcc tttttcaaaa acaacgattt ctgcatcttc cattaatcgg
    21661 cgaagccgtg tagcagcaga cattccacct gctacacctc cgacaataac gattttcatc
    21721 ttatttccct cctactgttc gatcaaacca ttgattcatg ccaccacgta cattcactac
    21781 atcataacca gatttcttta attctttagc tgcttgttta ctcctcatcc ctgattgaca
    21841 aatcacatag acggtctttt ctttgtctcc atcatatctg tttatacttt gtaatggaac
    21901 atttttagct ccttttatat gcccgccccg gtattctgca ggtgttctaa catcaagtaa
    21961 cgctggattt tccaaaagct tttcctttaa ttctgggact gttatcgatt ttattgattg
    22021 aaatagcaaa ttcattttct gcacctcatt tacattttta taaatacctc atggggtata
    22081 ttaaacagga gctttattaa tatgtcaacc agaatgactc ttcatttttt tcactacttt
    22141 ttatatttgc ataaaatacc gataaggggt attatctaat tataaaaata gaggagaatg
    22201 tgttatggag attgataata aaaatatcct gaaccgtttg aaacgtacag agggacaaat
    22261 tcgtggtatc caaaaaatga tcgaagacga aaaagaatgt atggatgtca tcactcaatt
    22321 aagcgctgtt cgttctagta ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa
    22381 aaattgcttt gagaaacctg atagtaatcc agaagaacaa gcggctaaac tagaacaagc
    22441 gatcaagatg attattaaaa agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg
    22501 atgaattaga aaaatggtca gaaacaggga aaaacgtaaa aaacctgata aaagcttctc
    22561 cagaaacgac gattgtcgta tctgtaaatg gcatcgcgat taccggttat ctagactctg
    22621 ccaatgcaga attcttagat ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc
    22681 gtgctaatca tatttcagaa agttctttac ctgaacaagt aatcgttgta ccagcaggtg
    22741 tccttgattt agttgaactg caatcccaag gttatgctta tatcaagcct tagtcagtag
    22801 aggttgcgac agaagtgatc agcttcaaga aatttcggct tatttttcga agaagctact
    22861 tctggaaaac cgtgaatcaa aggttgtgaa aacgctgtcc tgcatcaagt aataagaaca
    22921 gccaaacaaa aatagcttct catatttttc gtttggttgg gcttattacc gcagatgcaa
    22981 gcttttgaac accgtttaag gaagaggttg tgaaatcagc ggtttgacct aagtattaga
    23041 gaaaaaaacg ggaaaatagc tcctcatatt ttgccgtttt ttgagctaat accgaaggtc
    23101 agctgattga acaccgttta aggaagagat tgtgaaaaag tcgcttagtt tcgagtatta
    23161 agaaaaattt tgtgaaaatg gatttctaca tttttgcata aatttggctt aataccgaga
    23221 aactggcttt tgaataccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta
    23281 ttagagaaac aaacggaaaa atagctgctc atattttgcc gttttttgag ctaataccga
    23341 aggtcagctg attgaacacc gtttaccaag aggttgtgag cctaccattt aactccgaat
    23401 cacaagaaat aatttcaaaa acaacttctc ctgtttttgg ttcggttggc ttattatcga
    23461 gaagtagcta ctgggagtct atttatcaaa aaaaggaatg cgacataggt cacacccctt
    23521 ttttattttc tttgaaacat ctttcggtac tcgacagcat acaagacaaa agattgtaaa
    23581 agaccgtaag tgatgacggc aaaaatgact ctcacccaca gcgttggtaa ttctgggaaa
    23641 agcagcaaaa ggatcatgcc aacatagatg acagcagtcg ctactttgcc ataccacttc
    23701 gctccactta atttgatatt tttagatagc ccgattaatc cagctacaaa cataaagcct
    23761 tcttttatca agaatagacc aaacaacagc caaagccacg gtcgatacca tgccatagac
    23821 aaaatcagaa aaagttgtgt caatttatct gcaaacggat caagcacctt ccctaagtct
    23881 gttacctgat tatatctccg agcaatgacc ccgtctagat aatcagacaa acctgagaca
    23941 acaatgaccg ctcctgctat ataaaatgac tgtctcagca caaaataaag ataaatcggc
    24001 agcaaaaaaa tacggaatat cgataaaaga ttcggaatcg tttgtatttc tttacgccag
    24061 tctgtgctta atttcatttt atcgccctac ctcctcagca ctcttttctt tttattttag
    24121 cttacacggg gaataaaacc aatgatttca ctgtttttat ttttctttac agacactgat
    24181 attatgagct aatttgagat aatcacttgc tgttttcatc gtttactctt ctttcttcct
    24241 gtcgtttgcg catttctttt actagtactt tatgagcttt ttttgatcct cgctctaggt
    24301 cacgctgccg tttacgtgtt ttataatcga taaacagact gtctaaatcg taacctgtcg
    24361 ggtacagatc ttctgctgaa cgcaccagac gaacacgctg acgaggcacg tggattattt
    24421 ttttgtctaa atatacatca atcgtatctt cacctatatc ttcataaact aacgcttctt
    24481 tttggtattc attgacaaat atccgatcac cttttgcaaa gagctgacgt tcttttttat
    24541 tctccttcct atttgtaact gctgtcttag gaagaaattc ttttttctgt gtccgagagg
    24601 ctttttccgc caagtaacgc tctgcttgat ggatcaattt catcgacata tccattttat
    24661 gagcaatcca caaagcttgg ctttctcctg tttcttccaa cccgcaactg atatttagga
    24721 cgaagtgctt cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg
    24781 aatttcttga tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata
    24841 gattccatga ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc
    24901 tcatctaaaa gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc
    24961 atatgaccag aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg
    25021 aaaatctcat caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac
    25081 atcgccatca aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt
    25141 gctccggtga taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca
    25201 tgctcaggta aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt
    25261 ctaggtgtgc ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac
    25321 accgtgatcg tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc
    25381 aatgccgcca ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt
    25441 tcattcaatt tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca
    25501 tcgataatcg ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat
    25561 tcgccttttt gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag
    25621 aatttcagta attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta
    25681 cgcaaattac gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac
    25741 tcttctacat ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg
    25801 tattgattct tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat
    25861 tcgatcaaat cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg
    25921 atcctcggca accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct
    25981 cttgtttctt cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg
    26041 attcgttttt tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca
    26101 aattgtaatt gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct
    26161 gtcccaaaat aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt
    26221 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
    26281 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
    26341 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
    26401 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
    26461 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
    26521 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
    26581 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
    26641 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
    26701 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
    26761 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
    26821 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
    26881 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
    26941 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
    27001 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
    27061 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
    27121 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
    27181 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
    27241 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
    27301 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
    27361 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
    27421 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
    27481 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
    27541 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
    27601 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
    27661 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
    27721 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
    27781 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
    27841 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
    27901 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
    27961 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
    28021 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
    28081 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
    28141 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
    28201 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
    28261 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
    28321 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
    28381 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
    28441 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
    28501 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
    28561 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
    28621 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
    28681 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
    28741 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
    28801 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
    28861 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
    28921 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
    28981 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
    29041 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
    29101 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
    29161 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
    29221 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
    29281 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
    29341 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
    29401 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
    29461 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
    29521 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
    29581 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
    29641 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
    29701 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
    29761 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
    29821 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
    29881 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
    29941 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
    30001 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
    30061 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
    30121 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
    30181 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
    30241 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
    30301 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
    30361 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
    30421 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
    30481 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
    30541 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
    30601 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
    30661 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
    30721 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
    30781 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
    30841 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
    30901 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
    30961 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
    31021 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
    31081 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
    31141 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
    31201 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
    31261 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
    31321 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
    31381 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
    31441 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
    31501 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
    31561 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
    31621 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
    31681 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
    31741 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
    31801 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
    31861 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
    31921 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
    31981 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
    32041 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
    32101 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
    32161 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
    32221 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
    32281 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
    32341 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
    32401 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
    32461 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
    32521 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
    32581 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
    32641 ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt
    32701 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
    32761 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
    32821 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
    32881 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
    32941 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
    33001 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
    33061 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
    33121 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
    33181 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
    33241 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
    33301 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
    33361 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
    33421 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
    33481 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
    33541 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
    33601 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
    33661 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
    33721 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
    33781 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
    33841 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
    33901 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
    33961 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
    34021 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
    34081 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
    34141 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
    34201 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
    34261 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
    34321 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
    34381 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
    34441 tgggcttggc aaataaagct ctttatatgc tttttcaaaa ggtttcgtta caaattcatc
    34501 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
    34561 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
    34621 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
    34681 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
    34741 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
    34801 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
    34861 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
    34921 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
    34981 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
    35041 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
    35101 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
    35161 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
    35221 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
    35281 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
    35341 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
    35401 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
    35461 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
    35521 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
    35581 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
    35641 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
    35701 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
    35761 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
    35821 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
    35881 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
    35941 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
    36001 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
    36061 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
    36121 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
    36181 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    36241 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    36301 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    36361 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    36421 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    36481 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    36541 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    36601 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    36661 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    36721 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    36781 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    36841 atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    36901 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    36961 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    37021 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    37081 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    37141 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    37201 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    37261 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    37321 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    37381 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    37441 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    37501 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    37561 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    37621 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    37681 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    37741 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    37801 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    37861 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    37921 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    37981 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    38041 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    38101 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    38161 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    38221 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    38281 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    38341 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac
    38401 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    38461 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    38521 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    38581 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    38641 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    38701 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    38761 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    38821 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    38881 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    38941 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    39001 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    39061 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa
    39121 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    39181 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    39241 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    39301 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    39361 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    39421 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    39481 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    39541 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    39601 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    39661 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    39721 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    39781 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    39841 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    39901 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    39961 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    40021 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    40081 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    40141 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    40201 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    40261 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    40321 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    40381 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    40441 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    40501 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    40561 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    40621 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    40681 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    40741 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    40801 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    40861 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    40921 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    40981 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    41041 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    41101 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    41161 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    41221 aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct gttccaacaa
    41281 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    41341 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    41401 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    41461 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    41521 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    41581 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    41641 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    41701 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    41761 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    41821 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    41881 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    41941 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    42001 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    42061 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    42121 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    42181 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    42241 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    42301 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    42361 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    42421 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    42481 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    42541 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    42601 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    42661 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    42721 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    42781 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    42841 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    42901 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    42961 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    43021 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    43081 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    43141 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    43201 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    43261 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    43321 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    43381 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    43441 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    43501 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    43561 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    43621 cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt
    43681 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    43741 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    43801 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    43861 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    43921 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga
    43981 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    44041 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    44101 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    44161 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    44221 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    44281 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    44341 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    44401 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    44461 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    44521 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    44581 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    44641 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    44701 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    44761 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    44821 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    44881 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    44941 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    45001 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    45061 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    45121 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    45181 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    45241 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    45301 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    45361 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    45421 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    45481 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    45541 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    45601 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    45661 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    45721 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    45781 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    45841 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    45901 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    45961 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    46021 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    46081 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    46141 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    46201 catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    46261 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    46321 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    46381 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    46441 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    46501 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    46561 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaacac agccgatcgc
    46621 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    46681 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    46741 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    46801 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    46861 taataaaaca acgtgtttta tcatatacta aaacaaaaca taagtaaaca aaaaattcgg
    46921 attttaagca aaatccgaac gttccatctg attaaatccc tttatctcaa agttcatgaa
    46981 cattcacttt ggattacaag aatttcttta ttgtgttaat aaagtttctt cattctagtc
    47041 tcaaaaatca atagtgtgat ttgattttac catttcgttt ctgttcttct ttgatgaatt
    47101 cttttgtatc gacaggattc agattattta gtccgccaaa acctgtatcg accatttttc
    47161 cccagccttt tgtcttcttt tttcctgatt tgagattctt tgctcgaagc ttctcactat
    47221 gttttgctcg ccgatacttc aaataacctt ctaatcttcc cattagatcc actgtttttc
    47281 ctccttttta ttcaatcaga cgactcagct tttcctataa caaaataatt gccttcaggg
    47341 tcagaaaaat tgaaagtcaa gatgccattg ttatctgtga tttctcctgg ttcttccatg
    47401 ttctcgtaga tagtttcaaa ttcatccgta aaaaatacta aagaaggcgg tggtcctaat
    47461 acctctggtg aaaacttctg cacaaatact tttgggaata cgcctaattc taattctttt
    47521 gagatgctga gtacaatact cttaaattct tctggcaatt cgattgtttc agaaatttct
    47581 gcaccaattt tttctacaaa gaatcggcaa atcatctcta cgtcattgac ataaagcatc
    47641 acacgtgttt tttgtatcat catttgaccc ttctttctat ttgattttct ttcctgtcta
    47701 taaactgatt ccattatatc acgaaagcgc aaacaccttg tgtttgatac tattcaagaa
    47761 aaataaagca atagtttcta ggattgcttt cctccacaaa ctttttttac aaaaaaaaaa
    47821 cggcaatgaa tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct
    47881 ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac
    47941 gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc
    48001 accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca gttgagacag
    48061 cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg
    48121 gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt
    48181 cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta
    48241 ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc
    48301 agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga
    48361 cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct
    48421 tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg
    48481 ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa
    48541 ttggtatcca ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga
    48601 tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga
    48661 taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc
    48721 aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac
    48781 cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat
    48841 cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt
    48901 gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt
    48961 ctagtgcata agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa
    49021 atgtatctgt aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca
    49081 tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt
    49141 ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca
    49201 attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt
    49261 ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt
    49321 ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg
    49381 ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc
    49441 caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt
    49501 tgaattcgat attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga
    49561 tttcagccat ttcatcccac ctttattttg tttgtaagac tagttcacct ctttttgttg
    49621 caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat
    49681 tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat
    49741 aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa
    49801 aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc
    49861 acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt
    49921 atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc
    49981 tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca
    50041 ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg
    50101 aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc
    50161 gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa
    50221 acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag
    50281 tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg
    50341 actatgtaat caagtataga agactggttt aattacggca aataaatata atgaaatctg
    50401 caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct
    50461 gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat
    50521 atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa
    50581 cctccccact aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca
    50641 aacatgagaa gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc
    50701 acgacctctc ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt
    50761 tacttattgt cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga
    50821 gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg
    50881 tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct
    50941 tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca
    51001 cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag gcgaatcgtg
    51061 atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta
    51121 aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg
    51181 taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac
    51241 ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga
    51301 aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact accagccaga
    51361 agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat
    51421 aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt
    51481 atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca
    51541 gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg
    51601 ataatcttct tctttcacac ctgttcctcc atggataact agggggacat caactactgc
    51661 atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt
    51721 cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc
    51781 ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc
    51841 gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc
    51901 ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt
    51961 atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa
    52021 gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat
    52081 gccgatatat ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt
    52141 tgctgcgcct agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt
    52201 aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg
    52261 ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct
    52321 catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg
    52381 cacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat
    52441 tttccaaact cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt
    52501 taagtccaac ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct
    52561 cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt
    52621 tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca
    52681 tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata
    52741 agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgttgaat cccatcgaat
    52801 taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc
    52861 caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg
    52921 caaagaaatt ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa
    52981 acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc
    53041 tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag
    53101 ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata
    53161 catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat
    53221 ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg
    53281 ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca
    53341 acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac
    53401 cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag
    53461 tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga
    53521 taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg
    53581 tcgtcatcgt tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg
    53641 tgaaacgagc caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc
    53701 tacctaaacc caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc
    53761 cttgcatatg aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga
    53821 tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga
    53881 cttgtcctgc tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga
    53941 tcgatgcctg agtcaaaata ttttcgatga taaaattgat aacgcccatg ttttgatgtg
    54001 caccccaaaa gttagactag aaatctaatt aaaaggggtg ctttttttgc ggaaatatac
    54061 atttgttttt aagaaaaaag tagtttcaga ctatttaaat aacgaaggcg gctacaaata
    54121 tcttgcacat aaatatcaaa taaatcgtac actggttaga cattgggtaa ggatttataa
    54181 ctatcatggt tgggaaggct tggttggagg tggcaaaagc tacactacaa aatttaaact
    54241 tgatgttata gaatatatgg aaacaaatgg tctttctatc caagaaactg ctaaaaaatt
    54301 taatatcggt tcaaatagaa ctctaagtaa atggatagag caatatgaag aaggcggtgc
    54361 ttcttcactt gagagccaaa aaaggggcag aaaaattagt atgaattcca agctaaacat
    54421 tcctaaaaaa cttaaagatg agtctcttga agaagaagtt attcgtttaa gagcag
//
LOCUS       DAPBCS010000014        52611 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPBCS010000014 DAPBCS010000000
VERSION     DAPBCS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 52611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.014"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            339..902
                     /locus_tag="RD521_001007"
     CDS             339..902
                     /locus_tag="RD521_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770756.1"
                     /translation="MTAIYKALKNSNVSEYVKSRVLNNNLDYILENGVADLISKLSDP
                     IDIDHLVVWQDGTLEVSIYGEFYSRNKVTTFKNSISGREDLKGINKNSKFYRTNKELL
                     EKISKRVFELVNPFSKKRSKELFGIFYDGFSAKSNVEKFFYFNDSAYIAVKDSKRIPE
                     ATLEYLEPIKLSVFYSLYEKEIADEAV"
     gene            889..1773
                     /locus_tag="RD521_001008"
     CDS             889..1773
                     /locus_tag="RD521_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDT7770757.1"
                     /translation="MKRFKGSKMSKKIGKESLSLGLKKEIVKFKSMYREPEFRLIFQL
                     DIVRGLKISFSCLGYLVWLFIARVGWSNAYSFYKLFAGTDRLKESLEVKEALNILGSL
                     AFSLFIFALFILQVGKIMMSTNDNYINYLKPFQKGIKIICRQGTVLFLLSLYEHEIHF
                     QNYYNTTTKLGAFLLLYLVSNIAEKMLFKHIVKQVFSDIQKESYCLKTPYGNLKNVEL
                     FLFKMDSKWDKYIYRSKEVFLTGSYNKTLQESLYDTGAIFNNEPMVLKDFFGDYELVW
                     NPKQLSEQGAENEFAEVH"
     gene            2272..2526
                     /locus_tag="RD521_001009"
     CDS             2272..2526
                     /locus_tag="RD521_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770758.1"
                     /translation="MYNVGDQIDEIVKIGNVKLVKSKYYISETEIIFNTESGCILIWK
                     LPFPKDETTLKKINTLKIRYCIHEIIDKNTYVINQVKVMG"
     gene            2923..4524
                     /locus_tag="RD521_001010"
     CDS             2923..4524
                     /locus_tag="RD521_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDT7770759.1"
                     /translation="MLNSCEYADVLAKILKNGIKQFNSKNLVAFKKNSNKIGAIAGFT
                     SKTNMIKTKGLIYRSIDRVLEDSDVLTHWTPNVYSWLGGGENKTIFGHKEANLIQINT
                     FVADIDYPVGEKKTDLNLLVLYLLEEGLLPTLIVDTPKGYHCYFLIQNYNQETDSFDK
                     ASYISNSNDFKSLRVAKRISENIRKSIQNRLPQVDMGCNHFGIFRFPTKKNIVHYEPN
                     FVDTFEGYLKWSKDFEEKEYALKRSNLSLLAPNSTGKKGSKRQIDTKWYDFLIHTDIA
                     EGDRNKTVFTLALACKSSGLSIGECMDQLDQLQFNNDLSYSEVERTIRSAYRGDYKGA
                     KSSYVNEIIENYATPTEFEKYSINVNRQAVKRKGNRVDPSNWVKFAKPREERKYSHFE
                     ESKADLLAYLERKQRSLKKNELFLSVSMGTISEETQIPVSTLKVILKELRTANTVILQ
                     TSRGRYGSTKIATSKFVEAKILSTVIQNKKDKQVFVLELLGTANSHVETILRRYEDKT
                     VPFHANILKEREKSKHAPIRGANTG"
     gene            4758..5057
                     /locus_tag="RD521_001011"
     CDS             4758..5057
                     /locus_tag="RD521_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDT7770760.1"
                     /translation="MVIKKYVYSHPINVFIIGKLGLSVEVFCELYGFKQGTLSSWITR
                     EREVADLPISFLYALSLASGGNMNEVYDCLCEYEKEYKEYLKISAPKRNKKYIED"
     gene            5192..5551
                     /locus_tag="RD521_001012"
     CDS             5192..5551
                     /locus_tag="RD521_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HDT7770761.1"
                     /translation="MNIDGIYNESDFKKGDIAVIRSEYDFPSEIGIHTVEGKTVCRYW
                     GRKGNLILLLDTDSGDKLKFCFWHNTNYCCEPIPKRLIDVDKESVRYLADGTRIRLTY
                     QKSENASITRVRDIVVI"
     gene            5568..5786
                     /locus_tag="RD521_001013"
     CDS             5568..5786
                     /locus_tag="RD521_001013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770762.1"
                     /translation="MELIPYAYQHLYKAKSMKDGNEVVGALICCPPFSYIATIESMKK
                     MCVDELNDGEVKNLKLTRVLERSIEEFR"
     gene            5801..8164
                     /locus_tag="RD521_001014"
     CDS             5801..8164
                     /locus_tag="RD521_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HDT7770763.1"
                     /translation="MATVILAEKPDQAEKFAFALADKKIPKSGGKFEFQSEVFGDTIV
                     TWGIGHLVGLSMPEKYEWLPNKEKWDLANLPFLPKMDELKYEVSKGKSQQFAVVKACL
                     ENADQIIIATDPDREGENIAYNIFKLCKQSIFKKPMKRLWINSLVKSEIIKGFKNLRN
                     AEETISFFEEANARQIADYLVGMNYTELYTLKLQQKGLSGVVSLGRVQTPVNTLVVEN
                     DLSIKNFKSEKYKVIECHTVDREPKVIFKNSREYFNLDEFEADTKKYGLDSASIGIVK
                     SVEKTLKQVEPQKLFSLGGIQEYANKKWKYSAKKTDKIIQSLYDRGYLSYPRTDSELI
                     TTNEFEYLKANLDVYKSVLGISIDTPCLEPKKRFVDNDKVLEHYALIPTMDIPDIANL
                     TLDEKNIYEIVTRRACLMFAEPYKYENTKVTLDVNGLEFTTSGNVPISLGWKQVDTNL
                     DESDDKEETLPKFHEQETINIDIRFIDKLTKEPKRLTEGKLLGKTGIMAKLGLGTPAT
                     RSNIVDTLLNREYIKIENTNVYPTPKGYLLWDLTKNKDLLIGKPENTAIWEESLGKIA
                     QGSYTQEAFLRNIYNYINKTVSDLKKVEFTSDYLEKTVNQNKFDIGSYQVEEKKKVYS
                     ILNITSGEQFVIFKNISGKAINKKVLQELLLNNRTDKVLSGFKSKEGKAFNARLLFDP
                     NVMKVVMEFEKNSPKGDKKSKHDTVNGYEIVEKAKVFEIKELATGESFIFYKNNSGKT
                     LSSKTVKELLENGKTKEKVTGFISKDNKKYSAYLVFDNKNKVIKKEF"
     gene            8482..9081
                     /locus_tag="RD521_001015"
     CDS             8482..9081
                     /locus_tag="RD521_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III toxin-antitoxin system ToxN/AbiQ family
                     toxin"
                     /protein_id="HDT7770764.1"
                     /translation="MNLKNTNSNKSTLYVISDDYINKLKKVDYRVQNNYREQRLYFKS
                     NLKISNKNEEINYYVPLASAKENQRSLTNKSVFKIYGDREEKDFLGVLHINNMIPVPK
                     DCAKVWTPNSPNVDEKYKMLVIKQAKYLKLHESEINESCELLYNSKLGKLDPSYFKKN
                     RKEVITYQKIMTNVESLEAVSIAESQRNIHVQTQSIDVE"
     gene            9193..9417
                     /locus_tag="RD521_001016"
     CDS             9193..9417
                     /locus_tag="RD521_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770765.1"
                     /translation="MISMARPRKYNTEEERKEAMKKARKKWVEKNQETIAYIRAKSFA
                     KKFIEMSEDEDIELLEKWLEDRKNNGQNDE"
     gene            9799..10005
                     /locus_tag="RD521_001017"
     CDS             9799..10005
                     /locus_tag="RD521_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770766.1"
                     /translation="MIENRVLGFVECKDNLDDTLEVLESIIFNTKVLDLKAINGDMTT
                     HIILDEISAKEIGETLIAWANKEL"
     gene            10085..10939
                     /locus_tag="RD521_001018"
     CDS             10085..10939
                     /locus_tag="RD521_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7770767.1"
                     /translation="MMKSNFLGYVIAINMFKGGVGKSTLTQLMADILSARGFKVLVID
                     VDPQGTVTKKFQRQYDLKVVGKMTLFEAVINMSFLDCISPVKENLDIVLGSEQMSRFT
                     KVLRDMWRSTGFTDEGEEVFWNLFNYMIGNDNLRDQYDFILFDTIPTVSEFTDNVYVA
                     SDFLVIPTQTEQDSIDNLHSTVTNYQTAVKEVNGDLEILGIVPYLVDTKLKTGLELLS
                     ELQEEYGDLVYSHIVSERGVIKRWGREGLNKSIPHSKTAYSMYVDIVEETLVRLEKAT
                     GENILERV"
     gene            10952..11389
                     /locus_tag="RD521_001019"
     CDS             10952..11389
                     /locus_tag="RD521_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770768.1"
                     /translation="MMSKTKKVTSAGSNGSGNPFGIKSGSGGFNIGQQMLKGKAGIKS
                     TGDKRKSVRVPVELYDEVVKDLSYGAKYSLMTEILALFLKEFETHGNSIVTDFGKLKT
                     RPVKISEEAFKVLKVIKFETDQSNTNIIASALTWYLDKRKGKA"
     gene            11407..11706
                     /locus_tag="RD521_001020"
     CDS             11407..11706
                     /locus_tag="RD521_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770769.1"
                     /translation="MDHKSNYSYKENYIWKYVVAVFLLVGGIIALVKWAIIPNALVFI
                     NWFVPNFWSFFGYILIIWFMFKVLSFFWSPVYSLLGKWSYLILIATAIYIIMQIN"
     gene            11709..12152
                     /locus_tag="RD521_001021"
     CDS             11709..12152
                     /locus_tag="RD521_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7770770.1"
                     /translation="MQILEDIKIYWKRIIGYTLVLLVVNLFLSMIALVFVNQRKDNVT
                     ELVTFISFFISGIFAPFFEETLMRGIIQNQLELKTNLSTRSIYIIVAILFSLSHLNLY
                     FIPYFMTSLILSFVYDNSDKRLIVPVTIHCFYNLIVILLSVVFYT"
     gene            12297..12620
                     /locus_tag="RD521_001022"
     CDS             12297..12620
                     /locus_tag="RD521_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770771.1"
                     /translation="MARSRIAKIEQTKKDKKISKNKTKFFKAEDVKRIAWSVKTFDED
                     RYVKVSMNGLFVQLGNFSNFKTIDELYPEIRNIVDSKGLIVAKRKSRNSGLAYCKNKG
                     IMIPA"
     gene            13038..16667
                     /locus_tag="RD521_001023"
     CDS             13038..16667
                     /locus_tag="RD521_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770772.1"
                     /translation="MKKLKLISIALFSLITMSTARQSIVSAEGTSTKAVVEESGDKKV
                     AIDDKVKNEAEKATVVQGADGLMYVVVDENDPEKLLEAESPLKNQQENTGAVEVGVDE
                     KRQEKADKANEKVKNFDDIDYNDISSLQSALNDPKFPFLSEDELNEKLQKTKNTFESE
                     ALMKKTRGYFSQRILELSNNLNDVPKRAKVSRHALVSNRASQVSETRINPPNGYPWVI
                     YLIRSNGYSYTENYAIRRTIDGKEAFCLQAGVQATNTYTGSTSGYLSNSQREMISSIL
                     SAYKELGGWANNDAGKQRWIATQMLVWENSNEKPKSITVTPNWKNGATKWKNDLVARA
                     NAIRVRTKWDSTSKTVDEGDKATFKVTNTYGDHNLHVKSVSGGGKATISNGTLTVDTT
                     GATGNKITVDMIKGSHPSDAHPTTVWSNGSYQKLVTGSFYISTSVTVNVKRKGTVEVL
                     KVDSETKKPLSGATFRFSYSGKTKDVTTDSNGKAKLTERLKAGTVVKVKEIKAPNGYQ
                     LDSSEFSITVKENQNVTTTRTNKKSTGSVEIEKIGDLDGLGLPNVVFTIYNSDNKVVK
                     DNLKTDSNGKIKVDELQFGKYYAVEKQGVTGYDPDGKKYNFEISQDTPFTKPAKVKVT
                     NIYEVSISEKVSDMNESQVLKNNWSADPNDYLQYDITTGNLKKSGERNVQSFIIEGFY
                     DNKNVDVSKSEVYVGSTNVTNSFDINNDTNNGKVIAKAKTSVLTNNDFYNKSYNLRIT
                     MKIKKSTLENTEKQKNLTIVKNSKVTVGSKISKSASSNDVETVLYSRKVTINHIDEKD
                     KHLLKQDIDYKYDGETYEYKPRTDLFDKDGNNYKSDVIHKGKIDGKDIVLNTPYHIPV
                     LSVNVDRIQIDTARAVKNGYLPTKIELSKKEEYEKELEKIVFKVKITDIDNNKVVYDE
                     NFKYKEFNSHMSLDLPTEYLTKNKKINYSVDILLVDNPDKNKFETETKDLRTIGYTSS
                     EKVLTNKDMTNNQVNYSAVVRTVKERENNNPVKEFNESINYKFTDSTKTKTGYGISVD
                     LEPTYRNEIGKQIDMKVYSYPMNELIDSKIDEIYQSGGKETEVKMDRLKNDGKVESGI
                     STQNLSFQFPHMNIERKTGNIYADSQRNDESIKYELIDGGRKLYVPIWCDLGNYSIAI
                     KSSNFGGNYISLDMNQNIDVYAYMYATIDSETKKDDELLLEPVYPESTLPDGWSKNEI
                     DWLNK"
     gene            16727..17089
                     /locus_tag="RD521_001024"
     CDS             16727..17089
                     /locus_tag="RD521_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770773.1"
                     /translation="MRVQSYGNSVFDVKREFQDSRRKTKKLIAYFILSAIVFYIVKFV
                     LELNSIVFTDMVHKMLNGEPINFNFDYMLEKAQSNQIVAFIVSIYYFIEKSIFSLKDF
                     FFEMLTKLTTFYNKISKN"
     gene            17103..17255
                     /locus_tag="RD521_001025"
     CDS             17103..17255
                     /locus_tag="RD521_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770774.1"
                     /translation="MLDQNTDRSWWMIGAVIVGAILIGTAKVAFPEVFNIVITFFKNM
                     IPTNLG"
     gene            17323..17745
                     /locus_tag="RD521_001026"
     CDS             17323..17745
                     /locus_tag="RD521_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770775.1"
                     /translation="MIYVAFFLDGSDVMLRDMPKMILLMFVGFFLILVIAYYSLNGYT
                     QDSSVKGMQETLRTTAIANRDDSIRVEKGRFALIKSDFEKDFKEKFGKSKNLNNETAD
                     YKFDYLTDGKDGIKAIKVKVESGSKEYQATCILNVAND"
     gene            17757..19172
                     /gene="tadA"
                     /locus_tag="RD521_001027"
     CDS             17757..19172
                     /gene="tadA"
                     /locus_tag="RD521_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDT7770776.1"
                     /translation="MRVVEEKKISPKIEETPKSKINIASIIKKNGIKENSEAIEGNLD
                     FLTKLLEEDSGNYEPPIEDAKLNMIRDDLTRNYSQEVLQALTDDEIRRKLVQVVLKEH
                     GTFFKKDKEVAEYVVRECIGTGIIERIMTNDEITDVGWNGSQLSIETNSNKWLFDGDK
                     LNITQEYIERVISKYAVVNDRAFNSGNPIMDGMFKNVRISATHIDNSPDGSTFSMRVA
                     RPRLALNKENFEDFAPDYILELFEKMMKTKANICISGETGTGKTELQKLLLSFIDNHD
                     RMIMIEDVQETHAKELFPDKDIFSWITTPKKAISDLVKASLRNNPRWIIVSETRGSEA
                     YEMLQAVLSGHYVVTTLHAVNARAIPRRFVNMCASGFKINENAVEEDINRYFDFGVHI
                     KKTVVDGKVIRYLNEIVEFSASEPTTVFKQKFINGKFVIENGSLSEDFKEKMAEKFIT
                     FEFPEDQTNTEESLPEEEEVI"
     gene            19189..20004
                     /locus_tag="RD521_001028"
     CDS             19189..20004
                     /locus_tag="RD521_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770777.1"
                     /translation="MTKRKGLMDVSELNAYQVAYGKPLDYRDYLNYVGLPCLFMGGFS
                     FALCYYWWIALICGVIGGVYGFKVIMPKSIKRSYELKSLQERNKFINNMTQILTDDSK
                     TVTRALSIAKQRTKGELREDIQILESRLQGADKFQIAEAFREINNKYYSDVVFTQYFE
                     QLETAIYEGRNNSDTMKQIKSYHNDVKRKTEGFMKIKDGYLQDMKQMMFIVAVFIGAI
                     TFAFGFSTFYEGFARSPIGWVFGSIYFLIMVSFMKSFFKHYFDDEIMSLGVSN"
     gene            20004..20801
                     /locus_tag="RD521_001029"
     CDS             20004..20801
                     /locus_tag="RD521_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770778.1"
                     /translation="MKVKALKKKRKSSISPLKITKTEHKSFWIKLLGDVYYQKLREMN
                     NSNIAIIKWQKQRLKKSLIFILVGFLMYLFLGKEWYYLAGGIATSVGFYVWQAKSING
                     IYNQFRFERHLQFSKFTRLLIPYLKQKKDGGNLYGVFAKIVKRLDYDTDKKLLMKLMQ
                     EMTDQPNDIRPFTEYAEQTSGTDMSVLFMSTVYDIRQGSADLDVIEELDRLASEELMA
                     GIDSIIDYKSRRFVFFPTKIAMTSFILVVGFAVGVLFMNLKDINFGQ"
     gene            20819..21181
                     /locus_tag="RD521_001030"
     CDS             20819..21181
                     /locus_tag="RD521_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HDT7770779.1"
                     /translation="MQEQSVMEIIKWFIGLMMIMAMVSIGLFCVQLQDINTFKQQVNY
                     QIERKGGLTTEAVEAIDKYSATNYDRRFTIESDKLNQKVSYGEEIDYTIKATIPIQIL
                     PLPDVTLSFNGNSVSQIR"
     gene            21195..21341
                     /locus_tag="RD521_001031"
     CDS             21195..21341
                     /locus_tag="RD521_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770780.1"
                     /translation="MQENNVSRIIYLIGGLIILSLAIAGLVEHWPEIKLLIRNFYDNK
                     MIKM"
     gene            21353..21574
                     /locus_tag="RD521_001032"
     CDS             21353..21574
                     /locus_tag="RD521_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770781.1"
                     /translation="MEAIQENRKDNQETSEKNKKKRKKKVDKFTEMTDKIIDKKGLKP
                     KDFDRQAIKEARQKLYDDFVKENISQLLD"
     gene            21593..21868
                     /locus_tag="RD521_001033"
     CDS             21593..21868
                     /locus_tag="RD521_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HDT7770782.1"
                     /translation="MKNLVISKFAMLPRMDFFDNVSKFMSNMTSKLQGISLSVIIFCI
                     VVTGLMFLFGEGPSRTAKKWLTYIVIGAIIIFGASTIGQTIKSVGGF"
     gene            21977..23803
                     /locus_tag="RD521_001034"
     CDS             21977..23803
                     /locus_tag="RD521_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770783.1"
                     /translation="MNQRFEYNFEDVEPKLLKKGKGLKKKNKYTLKQAEALVVWIQHN
                     WSDKTTITLICSDTEEGTKYAFDGIEITPFSIGGQEKLLFPDIEGQILQAPDSMISDP
                     NKLILDINDQYKHEPDVAEPTKESKSKLNLKLPSVVLPFAKKKNKLMNELIEPEETDI
                     YEEELEEQEEIEELVVSSEETEEVEEIETVSENEEETEEIFDFEDEEEVENVQKGDLS
                     TSSQNGLEAVNDPQVPPYISSQTPNYSPQYQAPNVLQPLRKHETVQLVTLKEYCDLGE
                     EIETSISQMVEKLKPESLFKFIGIPSTGLTNTRIDEYRSNHAMLRLAEVKFENLREHY
                     SRQIISFKAEALDRLRTAVDIAWKKPYDLDVKKNKAEELSKMEAEAEAKLNEFVKRQE
                     NNVNDKIKKFEIEQEIDLKNFIARQEADKAVFIATEKDRTQNLIDSKAESINADLLET
                     KDKFIDEEMYKMKIEMNKNLFDGKQKIKSELATELAKANDNVWENALKFIQEIQDEIE
                     AKTPIWAAEIKEFNSLEEQEHERMKQTKELQLKEESIEIKRLEAEMNLKRMEELDHEN
                     KMLKIKLETALSKNELLAMEQDTKQVVNGSNRVGALNLFSKR"
     gene            23816..24532
                     /locus_tag="RD521_001035"
     CDS             23816..24532
                     /locus_tag="RD521_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770784.1"
                     /translation="MSVFNNKVMLGNCYVNHIGFYDGGTYRVLFLRLSVFSYSKDGKG
                     HYENINAQISCPLDRNGETTTTSSHPKALMNKFIFEKLDRNLVEGMKISIHGNIRGTE
                     QVLLNSGWKNFGQLSPLEKEEYQKIPKNELVTRNQTYIYIEEVSMPEKEEFRKSQSKV
                     TKEDVEVAEPNQADEILSDDTDPVNDEGEVIEVFEAEIIEEEEEIKSFDKRMYREKPK
                     GYVNFEDMYYDEYSNEPPIN"
     gene            24545..27403
                     /locus_tag="RD521_001036"
     CDS             24545..27403
                     /locus_tag="RD521_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDT7770785.1"
                     /translation="MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIE
                     GEDLPKSLQDKLNVPLVFIGVFLGSIVFLAITYLLNFLKALLVAIGQSESGLLGVEFD
                     PKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNHESEKNLVYGQKGDSRLSTIK
                     EIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGKGETFVVPL
                     LDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVI
                     DSWANKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDC
                     IEKITMYNVAQMLNELGSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGD
                     KAKGSILSTASQGIEMFTSDLFGKLTSKMSIDLKEIGFPKSIQFKVNTNLVGKRISIS
                     FLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLDIRYEEEKSRAWYRIEFPK
                     KQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNHVLASIF
                     ISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVI
                     QSYSQLETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLD
                     TDSSVTSSAEQERVITVERASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMP
                     YRYQFLAEDFDTQRDINEIDIACEHINLSLQDNQIPYARFIKDLKTRLEYSIINNIPI
                     SEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLSEQEMKINIYLKESQKE
                     AIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQIMIL
                     EQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI"
     gene            27454..30090
                     /locus_tag="RD521_001037"
     CDS             27454..30090
                     /locus_tag="RD521_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770786.1"
                     /translation="MQWLKYKWPIIVSLIIILFMAYPQRVFALPWITDNKEVLKFLTK
                     YQDYLQFTKGGFGSIVVQSLSWGLIRGAYFVCKSAENLVPQSLSLFSFVESSSLGAVY
                     QSVLNTVVVGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALILLMPTMISSGIQ
                     FAKTFYKDSTTITKADGGVAWSLIEDGVTDLVYINKNDAYDRLGKDGVQKNALKEKNF
                     FSYDFSEVITVDTAKDLEDDNKKAEYLQSRLTPDGEGNMQAVKIDDGWMSMFSDSFKG
                     GYYRFTKNTFALTLGLLSLAVAYVFSIFIIVTAILELAFKRVLGVLVFATDIETGQRS
                     KVVLSDILQCYLTVGFQGFGLSMFAMFVSYLGTGGGSSVNPFIKAVAYVCAVFVLIKG
                     SGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGNGNGSDSGEGED
                     RKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDMAND
                     TKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDRKDGAKVENADR
                     IMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKSAEELINQKRQE
                     AKYTPEAMNREEMLRKRVEGRTGTNTDEKEALTKERIQEAKNSNTGLEKLVKENLQNS
                     TSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIGVRENLQSSSTSQ
                     PKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGFKTVD
                     VRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFT
                     YEDNELFRDTLNDPNPLFDNLN"
     gene            30114..30449
                     /locus_tag="RD521_001038"
     CDS             30114..30449
                     /locus_tag="RD521_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770787.1"
                     /translation="MPAFYEVKMMYRNPKNIKQEIKLFFFYLVDLGIVAGSVMASVYF
                     AKLMPISPMMKFALYLLAFVVGVWMCVRTPNHPKERNILMLYYLFKMDRNRYHEVEFK
                     RNSRGKIEL"
     gene            30446..31063
                     /locus_tag="RD521_001039"
     CDS             30446..31063
                     /locus_tag="RD521_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770788.1"
                     /translation="MIVAESNENKLSGVFDKFLELVIPKDKDMCELLPYKKILKDGTL
                     VTNDNKYQAFFKVKTTDLTSISEDGFMRMVNKLTYLNRVYLEPYKIYSLTYPTETVNQ
                     AIYWKHRIQEAKRSLLSNNDPNLETYLKWNLKLAYDNYERVDLVSTLPELMFVICVYG
                     KTQKEVKNHADVMKQAGGRELDLQRITEGDLINVITKLMNPNTSL"
     gene            31079..31546
                     /locus_tag="RD521_001040"
     CDS             31079..31546
                     /locus_tag="RD521_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770789.1"
                     /translation="MLKHCRKGFCKVIKGKNAADLIFEWKESNLINVRGKNGSGKTER
                     VMASIDNSRPRSRALVITDEKNHYKGIRTIEKKYLTNNDLKVVDDILNIYPDFNLVVF
                     DETIPVTEIKGFYQSFSNLANKYEEVLFVWITEEDSNSKSAFFSEKNAFLHSI"
     gene            31562..33517
                     /locus_tag="RD521_001041"
     CDS             31562..33517
                     /locus_tag="RD521_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tra protein"
                     /protein_id="HDT7770790.1"
                     /translation="MIVSKKLARNLVSEGYDLRLVEKYQPSGGIKFDERYISTGDGYV
                     TSIAVIEFAEDVNSRWLTDIMAIPNTIATFDVCTADKEDLLHKINTSIGELDYRSTNE
                     NRTTDKTDALLDKYNLEDFAQEITKRGEVVKTVHARIFVYSDSLEELEKRISEIRKDL
                     ASVNHKAIVPQFRMKDEWMSLFQDGMEQESKLFAKKGLTVPAKNIGGGIPFHHKSLKD
                     PRGFYLGTTSTGGAFIFDPFYSTKQRRSFNGFILGKMGFGKSTLIKQLQEGLQAKNCF
                     IRGFDKSGEFEGMVKKAGGVYVDLGGGDMVVNPLQIYATVMKKDQFGNDVVDESKSFQ
                     NHITKCSNQIRFLEPEIDTATVREYTLMLQDFYIHYGILPKEWREARDDNERNQLNTN
                     ICNLPNDSYPIYGEFLKFIENQQFKIKNATPERVRSIELIKLIVTDMCTTYPQIFNGV
                     TTMPNFDTTKIVYYNIDSISKMSAPVFHCQLFTALTTIWNSALQNGKIQKENFKKYGS
                     DYYKYAQDFMVLIDECHNVINSNNKYACEYVLSFEREMRKMRAGVYFATQTPEEIIPK
                     DSGSEVADVIRNIFNLCTFKCFFNLDSALLEDIKKVLGNTITDTEIMLLPELEVGQAV
                     VQTSSEDTYMINFDPYPEQIERFDGGQ"
     gene            33539..34675
                     /locus_tag="RD521_001042"
     CDS             33539..34675
                     /locus_tag="RD521_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDT7770791.1"
                     /translation="MEQNKNNNVASTLARQGMKQVSKKIGLALKIKIGVVIGLITLVG
                     LLVIGSLATLQGTQTATNTGGAEVIGGNKPLSPEVQKWQKTVEKYAEKYGIKELVPYA
                     LAIMQCESGGKLPDLMQSSESAGLPVNTLQYEESIDQGLKHLSNVYNIAKSYGMESNY
                     DGIVQAYNYGIAYMHYLGKNKKTHTIETAEAYSKNVVAPSLGNTTGQTYSYVNEVSIK
                     YGKTYLYLNGGNFYYAELVKQYIGGGSGNPANPSEFWGKMKTEMEKYQGWPYVWGGKN
                     PTTGFDCSGLTAYLYQFSAGITIDSYTVTQYEATEAVSIKEAKAGDLIFFRGTYGAPD
                     FISHVGIYVNETTMYDSNSSGIGYHNWQDSYWQSHSPEIRRVKK"
     gene            34690..35340
                     /locus_tag="RD521_001043"
     CDS             34690..35340
                     /locus_tag="RD521_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDT7770792.1"
                     /translation="MIKRKNDNKWKYICGVLIIILVLGGLLSQLHTSNIQAKNKKLDQ
                     EVTQLEEQNSKLSKNISDLNANVEANLTNDDGSELNKQIAEVAKKFVELYPVYDIEKV
                     EEKKADLEKIATPSVADSIVPDDMITTSKKTLESKSTTQKGEAYSSDPTFKSKYKDSK
                     LYTNYVNANTINYFAEVNYKTESSSGDTENTVYMLFEVTNKDGQVLVDNYEIKYLK"
     gene            35354..36250
                     /locus_tag="RD521_001044"
     CDS             35354..36250
                     /locus_tag="RD521_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770793.1"
                     /translation="MNKKYSIHNKKEPNKKNKAQIDKVYKKTKGVKLYLGAILGFLAI
                     GMIIVFSPYVMGKSYDFETVKMSSAQNVTSSLNMYVDDMEINREAGLFKLVLRYQDDT
                     GTKSLSNIKSDYKLNFITNKNNEDVKTKVVKLSDEYTVIYYQNLPKDFGVISVTVNPR
                     YIYPELEPSNDLKEKEIKFYAVDKDIKSNAKLEVESTSDLQKDNFKYQIKAIENSIKD
                     EKKSIEKMNLANKVVQKDIDKANQNLEFQTLDEQTDTKNEISSMQTTIENNKSSIEES
                     ENKIKQFEEKIKQLEKSASRFE"
     gene            36286..36516
                     /locus_tag="RD521_001045"
     CDS             36286..36516
                     /locus_tag="RD521_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770794.1"
                     /translation="MCNSIVTIQEKAEEELMRIEAEYKEKRKAIKRKKNAELKKYKEE
                     QALKLGMMLCKYYDCYDINEVLKKANLNQFCE"
     gene            36867..37163
                     /locus_tag="RD521_001046"
     CDS             36867..37163
                     /locus_tag="RD521_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770795.1"
                     /translation="MDVSIRGIDPQYVKEIDKKVNKIQERTGKKFSRNDYFLAIIRQD
                     SEIDLIDYKKSEFDLVVEKLLLSNEQMVESISELTSVYERLFNFLISERSDLNK"
     gene            37160..37627
                     /locus_tag="RD521_001047"
     CDS             37160..37627
                     /locus_tag="RD521_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770796.1"
                     /translation="MNYPYFKVSATEDVKEKFNKCFEVNKSVFQTKSNMFRYVVSNLP
                     MLTKANQNFRDPEKEELAKNLEEAKLKISELETIIELQKQQLENFNKKEDSTHKDYSE
                     QLNSIEESVYKLLINSGLASKELEEERAEPEQESSRLGEGTMFSSPSLRFTTD"
     gene            37694..39229
                     /locus_tag="RD521_001048"
     CDS             37694..39229
                     /locus_tag="RD521_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770797.1"
                     /translation="MVGDDMTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDY
                     ISRQEAIRQDKLDHDYSEDELNELARIEKALEKIEQETDQPVIKDLREMDKYIDYMTR
                     KKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNGSVMFQDVISFDNDFL
                     VREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHVTAM
                     ERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLEL
                     KQSVKDSLIQLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNY
                     MTKDNPKRKEYDRMTKEIDELYQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLL
                     KQSEEKNLKSNELKNLLDGKKLNVKFDREKPEFKTKYAKQSYDDFQKRLEERQLAREK
                     NEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMIEQEKMRQAREFEI
                     GMN"
     gene            39251..39580
                     /locus_tag="RD521_001049"
     CDS             39251..39580
                     /locus_tag="RD521_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770798.1"
                     /translation="MKNFDIVCSNTKNIYLRELLNSDSETIEDVKKIIVLFEKENMEL
                     ENWGLFEIPISGNYCFYNWKTEDDVAFANYFFDKNYFSPLYIDKHSNEQVASSIKEAI
                     KLERVRK"
     gene            39582..39845
                     /locus_tag="RD521_001050"
     CDS             39582..39845
                     /locus_tag="RD521_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770799.1"
                     /translation="MIDFRTVMDKLSNGDFRVLIELYLNVTSTVKEFLYSNPKTALVL
                     VAVLIVFAVISFIKKMIPLLIIGAVLILGLYFPEVLDQIKNIF"
     gene            40020..40886
                     /locus_tag="RD521_001051"
     CDS             40020..40886
                     /locus_tag="RD521_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770800.1"
                     /translation="MKKLRVIATIPMIFSFALLLSACGKEEAGAKSNEAHNISFIDSA
                     EQKDSRCWLVVETSSKNKTPIDKNEYVKAVIQTKDGKAKVFKTDMEQLTLEKVSKMSD
                     EEIIEQALNADKQKFNDKKKSDIDSYNATLDSVKTELENEEKQEFQNDQKIADFQDSI
                     PKIQADIEEIDGVQYSEPEWKMVSATIKMDNENKSPSMETVKLSYKYLDSPIWNLNSF
                     GEVSSEGELSYESTSMDLKLSIPVATTKVLDNFYSGYSNDELDQLLLTKVASKDNKVQ
                     LDSKGKNVSIVE"
     gene            41041..42867
                     /locus_tag="RD521_001052"
     CDS             41041..42867
                     /locus_tag="RD521_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDT7770801.1"
                     /translation="MAYKKGNWKPKTKEQKQKELDDLIKASNSKIEQYQSNPEDMIEF
                     AKFMSKIHNYSPLNLSLIDEGFEGAIAVASYDGWKKLGFQVKAKEKGIGIYAHAPVTV
                     FIDENGEEKTLKEATSEQKEKIKNGELRSKKIKHFKKGYVFDISQTNATEADLPKIFP
                     NRVWNFNIDGELSVSQLEKGVNHLAESMNISIKDMNESGRGEMGSARGEYIQYVDGRE
                     EIVLNSRNSRTQNLTTSIHELAHKKLHNKNEHGSSYSTPIKEFQAEMTSFIVCHNYGI
                     DTSDFTIPYIANWTKNNEKIDPKNFRQIMQEVRETSIDFISTIDKKVLEEQQTAFNAL
                     KNSFVPNENKEQIQQKSDLEFHNFYDSTINDPDRTIHGSYLEAMVDYYRGKYGENSIE
                     LTHSNYDVTAKIGENYVTYYSDTKQFDVCDVYGVVFENPSENKNDRLLLDRSPENCDY
                     VEKFVVESLKACENHELKNDTLKLSEPLRSVVINEAVYNLSENANNYYNEKCWQEEIE
                     NQTNINDVKELNSCMTAEDFYESHKQEIENTITDFESVSGTNLRDTIDNESYKEVASV
                     VAYKLVATKVKYEMDEGTFKIPENSNLEKPTVKTAERSFEME"
     gene            42875..43948
                     /locus_tag="RD521_001053"
     CDS             42875..43948
                     /locus_tag="RD521_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770802.1"
                     /translation="MSNEQNYETGGTYRLFHGSMNKFDEFSLDHKGLNGTANGFGVYL
                     TPNKEMATMYAEQGSNGNNGFVYEVNVELEKPISMNERTITDEELSKVIDKLQESNDI
                     LNDYNDVSYYGEKVVKREVIASLSENENDVDLINDIGNTIGDHEATAKAFYDVGKYTH
                     IIAEEQLRQKDVVIVVLQPDKVKIETIHDLNEERNSLESSEDMTDKFVGHKVSEVPIR
                     EVVIKDFEQELETTNYYDKQAWQERIEYLENMEEPVSRLALDSDINEFYDSHKEEIET
                     KLTNLSEKLEIDKEELLGFPVDTSSTEYKKATSIMAYNGAMREIYNEIENDTFEIPPV
                     SEEMENKILKLTNFENTTKFEME"
     gene            43957..44217
                     /locus_tag="RD521_001054"
     CDS             43957..44217
                     /locus_tag="RD521_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770803.1"
                     /translation="MIEQENRNFLETQGEYRQINNSHFVICEDVQIAFKMALKYGKSI
                     RKKIIREKKSKKIGFWELSLRDGLNFIFCTEDYYEKYKCTLS"
     gene            44254..45258
                     /locus_tag="RD521_001055"
     CDS             44254..45258
                     /locus_tag="RD521_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDT7770804.1"
                     /translation="MDLKKAKKVSIVDFCKQNGIGLKQDSREYYRLEDHDSCVISDRK
                     NLFKWNSKDLGGDIFDFIKAYYDCSFKEAKQKLEDSDYEVHSFNTKVKKAPYQYQKEN
                     EVPVSVAKSYLVNERRIDTALVEDFLSKGLIKQDKKGNVLFLWKKDQEIVGCTEQGTR
                     KFFHEGKQKDVTWKKIQENSKEYSGFNVTFGQPDKMYFFESEIDLMSYITQKPEKAEN
                     ATFVSMNGLKVGNVLHGVTEHYNKFKSLPSETVLCVDNDKAGRTFIETEFKEKGIGRE
                     GIGISEVKVDVPLREGFDWNDNLTRGKEAFKRFKLEDVSKIVNFTHKLSLNTPVYEME
                     "
     gene            45283..45639
                     /locus_tag="RD521_001056"
     CDS             45283..45639
                     /locus_tag="RD521_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770805.1"
                     /translation="MNDTIAILSDSLIDLDKTSTDYMIIEEQLKNKNPEFDIQEKSLR
                     IELPKFKQKKLITILFEIIERYGLTYYQIWTDKKDGQYTKGVSIEAVREDDQVSIKAV
                     QPLMGYNEELLNCLNV"
     gene            45801..45992
                     /locus_tag="RD521_001057"
     CDS             45801..45992
                     /locus_tag="RD521_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770806.1"
                     /translation="MEQTRMFWSDRMQDHLDCIYKTREQLNKLDTVRYYFTYFSENDE
                     QRWDIFHDGNYEEFFYTES"
     gene            46101..46319
                     /locus_tag="RD521_001058"
     CDS             46101..46319
                     /locus_tag="RD521_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770807.1"
                     /translation="MVNKKETFKVGMEVQGVVIGSGELIEGEVISLLANVAVVKANGK
                     APVVMKKCSMMCLDGLNNPITQRFENVI"
     gene            46356..46673
                     /locus_tag="RD521_001059"
     CDS             46356..46673
                     /locus_tag="RD521_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770808.1"
                     /translation="MKDYASRKFIHLEPMKELVINGNEYNDYKSLFVKEQPKGGIRMK
                     NLPDFRLIEVNNLGIIEDYLAKLDEAVKQSIEKNGEDHVLTVVLKDLKDRLEAEAENF
                     NKD"
     gene            46684..46881
                     /locus_tag="RD521_001060"
     CDS             46684..46881
                     /locus_tag="RD521_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770809.1"
                     /translation="MRDMTEAVKELKKMYPDVLNMTVDDFHEALKNAESEEERTFYLV
                     LSSYVTRVDQKKVINQKDFKI"
     gene            46878..47846
                     /locus_tag="RD521_001061"
     CDS             46878..47846
                     /locus_tag="RD521_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7770810.1"
                     /translation="MILENDKPYYVVIGGVNGAGKSTIYQIQNSKRKELDTDRINADE
                     ILRRFGGNWNKQSDINKSMREVLREIEGHFKEGKSFHHETTFGGNPYPDKKRLLNAKK
                     LGYESFLIYIGLDSPERAIERVKSRVAKGGHGVPEDLIRKRYEFSLRNLAELEKYFDN
                     VSIYDNSDEFSYIYVKENHTVVANNTQEYAWVPKAIQEKNAETPISKQIMMDISQEEM
                     NPKETLERIYSASDDFISDRFSKEAQVDDFYRNHKNEIKERILSISFSTGVSQDELVG
                     QDKRRNSCKLAYINSVGELLVKTDSELLSMELNRDRNIQKTDFEVE"
     gene            48090..49262
                     /locus_tag="RD521_001062"
     CDS             48090..49262
                     /locus_tag="RD521_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770811.1"
                     /translation="MTKKIEVYVDGSQEERDSFFGFFWKNSREYVKALNMVYKQLMFE
                     ELIENSIKELDHEYIKRELSLEERIDNLLSNEVIDQKKLDKTKRELSKLRIAKNKEAR
                     DVLTQAIGLKSQTRIRDIAKKLDFDYSDFIDSAKMKASKDFSNDFVGIVTGQRTPRIY
                     RNKNKYGLLPFRGRDIKIHKADKFYISLPKGFRFKVNLGRKPRKARDSALTLERIMDG
                     QYKLNQSNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIMFTDLCSGKCLVNNQSV
                     IDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYGIEVKSE"
     gene            complement(49888..50265)
                     /locus_tag="RD521_001063"
     CDS             complement(49888..50265)
                     /locus_tag="RD521_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7770812.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(50311..50499)
                     /locus_tag="RD521_001064"
     CDS             complement(50311..50499)
                     /locus_tag="RD521_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDT7770813.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            50670..51212
                     /locus_tag="RD521_001065"
     CDS             50670..51212
                     /locus_tag="RD521_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7770814.1"
                     /translation="MKFGGHFLNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGD
                     CMKPNPVETGKRIKSIRSNFGMTMEQFAILTDSSNTSAVNNWERGYNLPNKTKLKKIA
                     ILGNTTTDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKDIQERYSDTFSLNK
                     DYELLDSIIKNIFTKYYSNG"
     gene            51407..52303
                     /locus_tag="RD521_001066"
     CDS             51407..52303
                     /locus_tag="RD521_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7770815.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            52352..>52611
                     /locus_tag="RD521_001067"
     CDS             52352..>52611
                     /locus_tag="RD521_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7770816.1"
                     /translation="MKPNLSLTSERIRKIIESKGLTLERFGDLIGDTPISSVNNWLRG
                     VSLPNKNRLLTIANIGNTSVDWIKWGTLEEYIASYLIDSGYEL"
BASE COUNT        20315 a         6452 c        10066 g        15778 t
ORIGIN      
        1 tagtaatatt ggtagaatta taatcacaaa cttaatagta attactattg ggattacaac
       61 attaacaaat aaagctttaa tatcaagtat gattttaata gtgtttgatt ttattatcat
      121 ttatagtttg ctgtttttaa taaagaaagt aactgaatat ttaaatcgtt aggcacgaaa
      181 taaattttaa ttgaggttga attatagcgg taaatcatac aaaatagaaa tgtagtcttg
      241 ttaagcgtct tgactatcta attttgaatg aggagggttg tatatagaaa aagggtagtt
      301 aaattcgtag atcaaaaggc tacgaaggag aataaagaat gacagctatt tataaagcac
      361 taaaaaattc taacgtatca gagtacgtta agtcgagagt attaaataat aatttagatt
      421 acattttaga aaatggtgtt gctgatttaa tcagtaaatt aagtgatcct atagatatag
      481 atcatttggt ggtatggcaa gatggtactt tagaagtatc aatatatgga gaattttata
      541 gtagaaataa agtaactaca ttcaaaaata gtattagtgg tcgagaagat ttaaaaggaa
      601 tcaataaaaa tagtaaattc tatagaacaa ataaagaatt gctagaaaaa atttcaaagc
      661 gtgtttttga attggtgaat ccattttcaa aaaaacgtag taaagaactt tttggaattt
      721 tttatgatgg ttttagtgca aaaagtaatg tagaaaaatt tttttatttt aatgattcag
      781 cttatattgc ggttaaagac agtaaaagaa tccctgaagc aactttagaa tatttagagc
      841 caattaaatt gtctgttttt tattcacttt atgagaaaga aatcgctgat gaagcggttt
      901 aaggggagta aaatgagtaa aaaaataggc aaagagtcat tatcgctcgg attgaaaaaa
      961 gaaattgtta aatttaaaag tatgtataga gagccggaat ttagactcat ttttcaatta
     1021 gatatagtga gagggctgaa aattagtttt agttgtttag gttatctggt ctggctattc
     1081 attgctagag taggatggag caatgcttat agcttttata aactgttcgc cggaactgat
     1141 cgattaaaag aatcattaga ggtgaaagaa gccttaaata ttttaggaag tcttgctttc
     1201 agcctgttta tttttgcttt atttatctta caagtaggaa aaataatgat gtcaacaaat
     1261 gacaattata ttaattattt gaagcctttt caaaaaggaa taaaaatcat ttgtagacag
     1321 ggcacagttt tatttctact tagcctgtat gagcatgaaa ttcattttca aaattattat
     1381 aatacgacaa ctaaacttgg tgcattttta ctcctatatt tagttagtaa tatagctgaa
     1441 aaaatgctgt ttaaacatat tgtaaagcaa gtcttttctg atattcaaaa ggaaagctat
     1501 tgtttaaaaa caccttatgg aaatttaaaa aatgttgagc tgtttttatt taaaatggat
     1561 agtaagtggg ataaatatat ctatagaagt aaagaagtat ttttaactgg tagttataac
     1621 aaaactcttc aggagagcct gtacgacaca ggagcaatat ttaataatga acctatggtt
     1681 ttgaaagatt tctttggaga ttatgaacta gtttggaatc caaaacagct tagtgaacag
     1741 ggggcagaaa atgaatttgc tgaagttcac taacatagtg tacagaaaaa cattatctga
     1801 aagaagaacg ccttcaaaag aaagtacaag aacgattggg ccgtacagaa gactaacgaa
     1861 agatcgtaaa aaaaaggaaa gacaatgaaa atgtctttcc tttttttttg actacaagta
     1921 gcctgaaaat gaaatttcaa aagactacaa gtagtctttt gtggcaaaaa taaagagcca
     1981 acgaaagcta ccaacaatcg ttgactcgta agactgttcg tagtaagaaa aatctttggc
     2041 aatccacgaa gtctttattt cataatttat ataaatgatt atacagtcac tttcctgaat
     2101 ttgcaagtaa aaaagctaag aaatattgtt taagcgtttc cttaaaagaa tctttaaata
     2161 aatttttttc caattgaaaa catgactaca agtagactat ttcttttatt ccaagttgac
     2221 tacaagtagt caaggtgata taatatatat atcaagaaag gagaaacaaa aatgtataac
     2281 gtaggagatc aaatagatga gatcgtaaaa ataggtaatg ttaaacttgt taaaagtaag
     2341 tactatattt cagaaactga aataattttt aatactgaat caggatgtat tctaatttgg
     2401 aaactgcctt ttcccaaaga cgaaacaaca ctgaaaaaaa taaatacttt aaaaatcaga
     2461 tattgcattc atgaaattat tgataaaaat acttatgtta taaatcaagt aaaagtgatg
     2521 gggtaaaaat cttttaaaaa aactcttgtt aaaagtaatt ctattagctt ataatgttgg
     2581 tattagttct gaagccaaag gaacactaaa aaattctagt agaaaagtta gttacagact
     2641 ctctactatg tatgaatgta acgaaaaaaa tagcgaaaaa gataattaaa taagactacc
     2701 aatcttattt aatggaactt ctgtagtaag aaaagcgatt tggcaatcca cgaagtccac
     2761 caagatgagc taccaactca ttttaatttt tgcacgttta aagtttagca tttaagtgct
     2821 tactctaaag acatagtgta attttaggac tccttattac ggaatctaaa gttaccctat
     2881 gtcttttttg ctattcaaaa ttgatagcaa ggagaaagtg caatgttgaa tagttgcgag
     2941 tatgcggacg ttttagctaa aattcttaaa aatggaatta aacagtttaa tagcaaaaat
     3001 ctagttgctt ttaaaaagaa tagcaataag attggtgcta ttgccggatt tacttctaaa
     3061 acaaacatga taaagactaa aggtcttata tatcgttcta ttgatcgtgt cttggaagat
     3121 tcagatgtat taacacactg gactcctaac gtgtattcat ggcttggtgg cggtgaaaac
     3181 aaaacaattt ttggtcacaa agaagctaac ttaattcaga ttaatacttt tgtagcagat
     3241 attgactatc cagtaggaga aaagaaaacg gatttgaatt tactagttct ttatctattg
     3301 gaagaagggt tattgcctac actgattgta gatacgccaa aaggttatca ttgctatttt
     3361 ctgattcaaa actataacca agaaactgat tcatttgata aggcaagcta tatttctaat
     3421 tcaaatgatt ttaaatcttt gagagttgct aaaagaatat cagaaaatat cagaaaatct
     3481 atccaaaatc gtttaccaca agtcgatatg ggctgtaatc attttggtat ttttagattt
     3541 ccaactaaaa aaaatattgt acattatgaa cctaatttcg tagatacgtt tgaagggtat
     3601 ttgaaatggt caaaagactt tgaagaaaaa gaatatgctt taaagaggtc taacttgtca
     3661 ttattagctc ctaattctac tggcaagaaa gggtctaaac gacaaattga tactaaatgg
     3721 tacgactttt taattcatac ggatattgct gaaggagata gaaataaaac tgtttttacc
     3781 ttagccctgg catgtaaatc aagtggctta tctattggcg agtgtatgga tcaattggat
     3841 caacttcaat tcaataatga tttgtcctat tctgaagttg agagaacaat aagaagtgct
     3901 tatcgtggcg attacaaagg tgctaaaagt tcatatgtca atgaaattat cgaaaattat
     3961 gctacaccta ccgagtttga aaaatactca ataaatgtaa atagacaagc tgttaaacgt
     4021 aaagggaatc gtgtcgatcc gtctaattgg gtgaaatttg ctaaacctag agaagaacga
     4081 aaatatagtc attttgaaga aagtaaagct gatttattag cttacttaga aagaaaacaa
     4141 agatcattga aaaaaaatga gttgttttta agtgtctcta tgggaactat atctgaagaa
     4201 acacaaattc ctgtatctac gttaaaggtg attttaaaag agcttagaac ggcgaataca
     4261 gtgatattac aaacgtctcg tggtcggtat ggatcgacta aaatagcaac aagcaaattt
     4321 gtggaagcta aaatattaag tacagttatt cagaataaaa aagataaaca agtatttgtc
     4381 ttagaattat taggaacagc aaatagccac gtagaaacga ttttaagacg ttatgaagac
     4441 aaaacagtac cgttccatgc gaacatactg aaagaaagag aaaagagcaa acatgcgcca
     4501 attagaggag ctaatacagg ttagagttct atttattttt ttgattttta aatagctaaa
     4561 ttgccaatta acatatctag tgtttctgct cactcctatt cctgttttaa cgtatactaa
     4621 tcattagtag ttagtactct cttttacgta tggagtgcat agaactattt ttacttgtgt
     4681 cttttccctt aatccacgtg tacggaatac agcttataac tgcgtatgca gttatgtttg
     4741 aaggaggaag attttctgtg gtaattaaga aatatgttta ctcacacccg atcaatgtat
     4801 ttattatcgg taagctaggt ttatctgttg aggtgttttg tgagttatat ggatttaaac
     4861 aaggaactct atcgtcttgg attactagag agcgtgaggt agctgatttg cctatttctt
     4921 ttttgtatgc tttgtctcta gcttcaggag ggaatatgaa tgaggtttat gattgtttgt
     4981 gtgagtatga aaaagagtat aaagagtatt tgaaaatttc agccccaaaa cggaataaaa
     5041 aatatattga agattagaga aagaagaaaa gaataatttg ctttttgact acaagtagac
     5101 tgatagctac tctgtttttt gatagtttga tattggctgt ttttttgtta gaattaatat
     5161 atatcaggta aataaaaaaa gggggctatt aatgaatatt gatgggattt ataatgaatc
     5221 agattttaag aaaggcgata tagcggtaat acgttcagag tatgattttc cttcagaaat
     5281 aggaatccat acagtagagg gaaaaactgt ttgtcgttat tggggtagaa aagggaattt
     5341 aatattatta ttagatactg attcaggtga caaattaaag ttttgttttt ggcataatac
     5401 taattattgc tgtgaaccta ttccaaaaag attgattgat gtagataagg aaagtgtaag
     5461 atatttagct gatggaacta ggattagatt aacgtatcag aagtctgaaa atgcttcaat
     5521 tactcgtgta agagatattg ttgtaatcta gtgggaggtg aaggattatg gaattaatac
     5581 cttatgcgta tcaacatttg tataaggcta aatcaatgaa agatggtaat gaagttgttg
     5641 gagcgttgat ttgttgtcca ccattttctt atattgctac cattgaatct atgaagaaga
     5701 tgtgtgttga tgaattgaat gatggtgaag ttaaaaattt aaaattaaca cgtgtattag
     5761 aacgttcaat tgaagaattt aggtgaagga gagagtattt atggcaactg taattttagc
     5821 agaaaaacca gatcaagcag agaaattcgc ttttgcgtta gccgataaga aaattccaaa
     5881 aagtggaggg aagtttgaat ttcaaagtga ggtatttgga gatacgattg taacttgggg
     5941 gataggacat ttagttggat tatcaatgcc ggaaaaatat gaatggctac ctaacaaaga
     6001 aaaatgggat ttagctaatc ttcctttttt accaaaaatg gatgagttga agtatgaagt
     6061 ttcaaaggga aagagtcagc aatttgccgt agttaaggcg tgtttagaaa atgctgatca
     6121 aattattata gccactgatc cagatcgtga aggagagaat attgcttata atatttttaa
     6181 gttgtgtaag caatctattt ttaagaagcc tatgaagcgg ttgtggatta attctttggt
     6241 taaaagtgaa ataataaagg gatttaaaaa ccttagaaat gcagaggaaa ctatttcttt
     6301 ctttgaagaa gcaaatgcac gacaaattgc agattattta gtgggaatga attatacaga
     6361 attgtataca ttaaaattgc aacaaaaagg cttatctggg gtggtatcac taggtagggt
     6421 tcagacacct gttaatacat tggttgttga aaatgattta tcaattaaaa atttcaagtc
     6481 tgaaaagtat aaagtgattg aatgtcatac tgttgacaga gagccaaaag ttatattcaa
     6541 aaatagtcga gaatacttca atttagatga atttgaagca gacactaaaa aatacggttt
     6601 ggattccgcc agtataggaa ttgtaaaatc agtagaaaaa acattgaagc aagtagaacc
     6661 acaaaaatta tttagtttag gggggattca agaatacgct aataaaaaat ggaaatattc
     6721 tgctaaaaaa acagataaaa ttatccaaag tctatatgat cgtggatatt tatcttatcc
     6781 aagaaccgat agtgaattga taactacaaa tgaatttgag tatttaaaag cgaatttaga
     6841 cgtgtataag agtgttttag ggatcagtat tgatacacct tgtttagagc ctaaaaagcg
     6901 gtttgtagat aatgacaaag tattagagca ttatgcacta ataccaacaa tggatattcc
     6961 tgatatagca aatctgacat tagatgaaaa aaatatttat gagatcgtta ctaggcgtgc
     7021 gtgtctaatg tttgcagaac cttacaagta cgaaaataca aaagttacat tagatgtaaa
     7081 cggcttagaa tttaccacaa gtggtaatgt acctatatct ttaggttgga agcaagtaga
     7141 tactaattta gatgaatctg atgataaaga ggaaacatta cctaaatttc atgaacaaga
     7201 gacaataaat atagatatta gatttattga taaattaact aaggaaccta aaaggttgac
     7261 tgaaggtaaa ttattaggta aaaccggtat tatggcgaaa ttagggctag gaacgcccgc
     7321 tacacgttct aatattgtag acactcttct aaatagggaa tatattaaga tagaaaacac
     7381 gaatgtttat cctactccaa agggatattt attatgggat ttgacaaaga ataaagacct
     7441 attgattgga aaaccagaaa atacagcgat atgggaagaa tcacttggga aaattgcgca
     7501 aggtagctat acacaggaag cttttttaag aaatatctat aactatatca ataagactgt
     7561 ttcagatttg aaaaaagtag aatttacttc ggattattta gaaaagactg taaatcaaaa
     7621 taaatttgat ataggtagtt atcaagtgga agaaaagaaa aaagtttatt ctattttaaa
     7681 tattactagt ggtgaacagt tcgtgatatt taaaaatata tcaggaaaag caatcaataa
     7741 aaaagttctt caagaactat tactaaataa tcgtacagat aaagtccttt ccggctttaa
     7801 gtcaaaagaa ggaaaagctt ttaatgcaag attacttttc gatccaaatg tgatgaaagt
     7861 ggtaatggag tttgaaaaga acagtccaaa aggcgataaa aagagcaaac acgatacagt
     7921 aaatggttat gaaatagttg aaaaagcaaa agtatttgaa ataaaagaat tagcaacagg
     7981 tgaaagtttt attttctata aaaataatag tgggaaaact ttaagttcaa aaacagtcaa
     8041 agaattgtta gaaaatggga aaactaaaga gaaagtaacg ggttttataa gtaaagacaa
     8101 taaaaaatat agtgcttatc ttgtctttga taataaaaac aaagttataa agaaagaatt
     8161 ttaattctta gtggcggaaa tcatttgttt ttccgcttat ttttattcaa aagtgcccaa
     8221 aatataggta tactgaatta aggataaata caaaactagt tagaggacag ttgaaaaaat
     8281 acaaacaaat tagattgcta ttttgttaaa ttggaagtag aataataccg ttttgatatg
     8341 tatagtgtgt tttaccaata aaattacata aacgtgaata tatttttatg aattaaatgt
     8401 aaaatgagag caattagttt taaaaaagat tataggctgt taatcttagc atatataatc
     8461 cattggaaag ggggagatcc aatgaatcta aagaatacaa actcaaacaa atctacacta
     8521 tatgtaattt cagatgatta tataaataag ctgaaaaagg tggactatcg tgtccaaaac
     8581 aactatcgag aacaacgatt atattttaaa tcaaacttga aaatttctaa caaaaatgaa
     8641 gaaataaatt attatgtacc attggcttca gcaaaagaaa atcaacgttc tttgactaat
     8701 aaatcagtgt ttaagattta tggtgatcga gaagaaaagg actttttagg agtacttcat
     8761 ataaataata tgattcctgt gccaaaagat tgtgcaaaag tatggacacc taatagccca
     8821 aatgttgatg agaagtataa gatgttagta atcaaacagg caaaatattt gaaattacat
     8881 gagtctgaaa taaatgaatc atgcgaatta ctttataatt ctaagttagg taaacttgat
     8941 cctagttact ttaagaaaaa tcgtaaagaa gtaattactt atcaaaaaat aatgacgaat
     9001 gtagaatcat tggaagctgt tagtatagca gaatcccaaa gaaatattca tgtacaaact
     9061 caaagtatag atgtagagta agataggcta aacctatctt attttattat tctattggat
     9121 attatgatac attgaatttt aatcgaatat gtatttaaat gttattatac tgtagaagat
     9181 aaaacttaaa ggatgatttc tatggcgaga ccgagaaaat acaatacaga agaagaaaga
     9241 aaagaagcaa tgaaaaaggc taggaaaaaa tgggttgaaa aaaatcagga aactattgcc
     9301 tatataagag ccaaatcatt tgctaaaaaa ttcattgaaa tgtctgaaga tgaagatatt
     9361 gaattgttgg aaaaatggtt agaagatcgg aaaaataacg gtcaaaatga tgaataataa
     9421 ccattgacta caagtagtca atggtgtaga atggttttag ggatagaaat atcccttttc
     9481 taaaagtcta atgactacaa gtagtcgata gagggatcgg gaataaaaac agacgattat
     9541 ggcctaaaga aaggaggaat atgaatattt tgaggaacga tttattaaat gagcgttgat
     9601 aaatttcact aaatattttc ttaatatcta tgtattgttc aagcgtagaa aaaaatattg
     9661 aaattattac aaaaccgctc ataatggaaa ataacgaatt atattaatga atcgttcact
     9721 tttgaccttt tttactagat ttttttgtag gacttttaga gaattttttt gttatctgat
     9781 tataaggagg agctaaagtt gatagagaat agagtactgg ggttcgtaga gtgtaaagat
     9841 aatttagacg atactttaga agtccttgaa agtattatat ttaacaccaa agttctggat
     9901 ttgaaagcaa ttaatggtga tatgacaaca catatcatat tagatgaaat tagcgcaaaa
     9961 gagattggcg aaactttaat agcatgggct aataaagagc tgtaaaagat aattttaaaa
    10021 taaagtggcc aattgactac aagtagtctg tatagtataa taaattatca aaagtgaggt
    10081 atacatgatg aaatcaaatt ttttgggata tgtaattgca atcaacatgt ttaaaggggg
    10141 agttggtaag tcaactctta ctcagctaat ggctgatatt ctatcggcta gaggctttaa
    10201 agttttagtt attgatgttg atccacaagg aacagtaact aaaaagtttc aaagacaata
    10261 tgatttaaaa gttgttggga aaatgacatt gtttgaagca gtaattaata tgtctttctt
    10321 agactgcatt tctcctgtta aagaaaactt agatattgtg cttggttcag aacaaatgtc
    10381 aaggtttacc aaagtattaa gagatatgtg gagaagtact ggttttactg atgaaggtga
    10441 agaagtcttt tggaatttgt ttaattatat gatcggtaat gataatttaa gagatcaata
    10501 tgattttatt ttgtttgaca ctattcctac agtttcagaa tttacagata acgtttacgt
    10561 ggcgagtgat tttttagtaa ttcccacgca aactgagcaa gattctatag acaacttgca
    10621 tagtacggtt actaattatc aaactgctgt aaaagaagta aacggagatt tagaaatttt
    10681 aggtattgtt ccatatctag tggatactaa attaaaaaca ggattagaac ttttatcaga
    10741 acttcaagaa gaatatggag acttagttta ttctcatatt gtttcagaac gaggcgtaat
    10801 taaacgttgg ggtagagaag gactaaataa atctatccca cattctaaaa ctgcttatag
    10861 catgtatgta gacattgtag aagaaacgtt agtacgttta gaaaaagcta caggagaaaa
    10921 tattttagaa cgtgtttagg aaaggtgttg catgatgtcg aaaactaaaa aagtaactag
    10981 tgccggcagt aatggttcgg gtaatccgtt cggaataaaa agcggtagtg gtggatttaa
    11041 tataggacaa caaatgttaa aaggtaaagc tggcattaag tctactggtg ataaacggaa
    11101 atctgttagg gtaccagttg aattatacga tgaagttgtt aaagatttat catacggggc
    11161 taaatattca ttaatgacag aaattttagc tttattttta aaagaatttg aaacacatgg
    11221 aaatagtata gttactgatt ttggcaagtt gaaaacgaga ccagtaaaaa tttctgaaga
    11281 agcctttaaa gttttgaaag ttataaaatt tgaaacagat caatctaata ctaatattat
    11341 tgcttcagct ttgacatggt atcttgataa aagaaaagga aaagcataat gaaatgaggt
    11401 aagactatgg atcataaatc aaattattca tacaaagaaa attatatttg gaagtatgta
    11461 gtagccgttt ttctactggt aggtggaatt attgcattgg ttaaatgggc tataattcct
    11521 aatgctttag tatttatcaa ttggtttgtc cctaattttt ggagtttttt tggctatata
    11581 ttgataatct ggtttatgtt taaagtactc tcttttttct ggtcgcctgt atattctttg
    11641 cttggaaagt ggtcgtacct aattttaata gcgacagcta tttatatcat tatgcagatt
    11701 aactaattat gcagatttta gaagatatta aaatttattg gaaaaggata ataggctata
    11761 cattagttct tcttgtagta aatttattcc tttcgatgat agcgttggtc tttgttaatc
    11821 aaagaaaaga caatgtaact gaattagtaa cgtttatttc gtttttcata agtgggattt
    11881 ttgctccttt ctttgaagaa accttaatga gaggaattat ccaaaaccaa ttggaattaa
    11941 aaacaaattt gtctacaaga agcatatata taattgttgc aattttgttt agtctatccc
    12001 acttaaattt atattttatt ccttatttta tgacgagttt aattttgtct tttgtctatg
    12061 ataattcaga taaaaggctg attgttccgg ttactattca ttgtttttac aatttaattg
    12121 taatactgtt aagtgtagtt ttttatactt gaaaaaaata gacgaaaacc taaaaataca
    12181 aggtttttat ctattttttt tattttgttt ttatctatca ggttgataga aattgtcata
    12241 aatgatataa ttaagtatga actgatttag taaaaaaatt aaaggagtga tagctgttgg
    12301 caagatcaag aattgctaaa attgaacaga ctaaaaagga taaaaaaatt tctaaaaata
    12361 aaacaaaatt ctttaaagct gaagatgtta agaggatagc atggtctgtg aaaacatttg
    12421 atgaagatcg ttatgttaaa gtttcaatga atggtctatt tgtacagctt ggtaacttca
    12481 gtaattttaa aacgattgat gaactttatc cggaaataag aaatattgtg gatagtaaag
    12541 gtttaatcgt ggctaaacgg aaatcaagaa actctggtct tgcttactgt aaaaataaag
    12601 ggatcatgat tccggcttaa atcagtgaaa ttgttctttg aaaacttaat actactcgta
    12661 ctgtagcaat gttgctatgt tacagataaa agcgtaaggc taataagcaa aatagcaacc
    12721 aagcatactg ttatttcctt tgccaagagg tatgtaaata cgttagcaat agaagtgata
    12781 aggttagtat ttcacgccaa aaaatacaaa agtcgatcca ttgaaactac taattatgag
    12841 gtggcgtaaa tggtaattat tcagaatttc tatctaggaa gaacctatga aaaccgtcct
    12901 agagatcaga atattgacac taaaaaattc agcacagtaa taaatgtgct agatacaggg
    12961 taactcttta ctacaagaaa tgtagtaaag agttgttatg attctttatt acatttcttt
    13021 ctaaaggaag tgtgataatg aaaaaactaa aactgattag cattgctcta ttcagcctaa
    13081 tcactatgtc aacggctcgc caatcaattg tttcggctga aggaacgtct acgaaagcag
    13141 tagtagaaga gtctggcgat aaaaaagttg ctatagacga caaagtgaaa aacgaagcgg
    13201 aaaaagcgac tgtggttcaa ggggcagacg gcttgatgta tgtggtcgtt gatgaaaatg
    13261 atcctgaaaa attgctagaa gctgaatctc ctttgaaaaa tcaacaagaa aataccggcg
    13321 ctgttgaagt cggtgttgat gaaaagagac aagaaaaggc tgataaagct aacgaaaaag
    13381 tgaaaaactt tgacgatatt gattacaacg atattagttc gttgcagtca gcgttgaatg
    13441 atccgaaatt ccctttttta tcggaagatg aattaaatga aaaattgcaa aagacaaaga
    13501 atacatttga gtctgaagcg ctaatgaaaa agacaagggg atatttcagt caaaggattt
    13561 tagagttaag caataattta aatgacgtac cgaaaagagc gaaagtgagt cggcatgctt
    13621 tagttagtaa ccgtgcttca caagtaagtg aaacacgaat aaaccctcca aatggttatc
    13681 cgtgggtgat ttatttaatt aggagcaatg gttatagtta tacagaaaat tatgctatta
    13741 gaagaactat tgatgggaaa gaagcttttt gtttacaggc cggtgtacaa gcgacgaata
    13801 catacactgg ttcaacaagt ggttatcttt ctaattcgca aagagaaatg attagttcga
    13861 ttttatcagc atataaagaa ttaggcggtt gggctaataa tgatgccgga aaacaaagat
    13921 ggatagcaac tcaaatgctt gtttgggaaa acagtaatga aaagcctaaa agtataactg
    13981 ttacgccaaa ttggaaaaac ggagctacta agtggaaaaa tgatttagtg gctcgtgcaa
    14041 atgctattag agtaagaaca aaatgggatt ctacgtctaa gacggttgat gagggagata
    14101 aagcaacctt taaagtaact aatacttatg gagatcataa tttgcatgtg aaatctgtat
    14161 ctggtggcgg caaagctacc ataagcaatg gtactttaac tgttgatact acaggagcca
    14221 ctggaaacaa aatcactgta gatatgatta aaggttctca cccttcagac gcgcacccta
    14281 caacggtatg gagtaatggt tcttatcaaa agcttgttac cggttctttt tacattagta
    14341 cttctgttac ggtaaatgtt aagagaaaag gaactgttga agttttaaaa gttgattcag
    14401 aaacaaaaaa accattatca ggagcaacat ttagatttag ctactcaggt aaaacaaaag
    14461 atgttactac tgattcgaat ggtaaagcaa aattaactga aaggttgaaa gctggaactg
    14521 tagtaaaagt taaggagata aaagctccta atggctacca actagattca agcgaatttt
    14581 caattacagt taaagaaaat caaaatgtta ctaccacaag aaccaataag aaatccacag
    14641 gttctgtaga aattgaaaag ataggcgatt tagatggatt aggactaccg aatgtagtat
    14701 tcacgattta taattccgat aacaaagttg taaaagataa cttaaaaaca gatagtaatg
    14761 ggaaaataaa agtcgatgaa ctgcaatttg gtaaatatta tgcagtagaa aaacaaggtg
    14821 ttactggtta tgatcccgat ggtaaaaaat acaattttga aataagtcaa gatactcctt
    14881 ttacaaaacc ggctaaagtg aaagtaacaa atatttatga agtatccata agcgaaaaag
    14941 tatcggacat gaatgaaagt caggtattaa aaaataattg gtctgctgat ccaaacgatt
    15001 atcttcaata tgacattaca actggaaatc taaaaaaatc gggagaaaga aacgtccaaa
    15061 gtttcattat cgaagggttt tacgacaata agaatgtaga tgtgtcaaaa agtgaagtat
    15121 atgttggatc aacaaatgta acaaactctt ttgacattaa taacgatact aacaatggaa
    15181 aagtaattgc taaggcaaag acaagcgtat taacgaataa tgatttttat aataagtcct
    15241 ataatttacg gatcactatg aaaattaaaa aatctactct tgaaaacact gaaaagcaaa
    15301 aaaatttaac gattgttaaa aatagtaaag ttactgtcgg ttcaaaaatc tctaaatctg
    15361 ctagctctaa tgatgtagaa accgttttat attcaagaaa agttactatc aatcatattg
    15421 atgaaaaaga caaacacctt ttgaaacaag atattgatta taaatacgat ggggaaacgt
    15481 atgaatataa acctagaacc gatttatttg ataaagatgg aaacaattat aaatctgatg
    15541 taatccataa agggaaaatt gatggaaaag atatagtttt aaacactccc tatcatattc
    15601 cggtactttc tgtgaatgtg gatcggattc aaattgatac ggctagagct gttaaaaatg
    15661 gctatttacc tacaaaaata gaactaagta aaaaagaaga atatgaaaaa gaattagaaa
    15721 aaattgtatt caaagttaaa attacagata tagataacaa taaagttgtt tatgatgaaa
    15781 actttaaata caaagaattt aatagtcata tgtcgcttga tttacctaca gaatatttaa
    15841 caaagaataa aaaaatcaat tatagtgttg atattctttt agtggataat ccagataaaa
    15901 acaagttcga aactgaaact aaagatttaa gaacgatcgg ttatacttct tctgaaaaag
    15961 ttttaacaaa caaagatatg acaaataacc aagtcaacta ttcagcagta gttagaacag
    16021 tgaaagaaag agagaataat aatccggtca aagagttcaa tgagagtatc aattataaat
    16081 ttactgatag cactaaaaca aaaacaggat atggtatatc agttgattta gagccaacat
    16141 atagaaatga aattgggaaa caaatagata tgaaagtcta tagttatccg atgaatgaat
    16201 tgattgacag caaaattgac gaaatctatc aaagtggggg aaaagaaaca gaagttaaga
    16261 tggatcgctt aaagaatgat ggtaaagttg agtcgggtat tagtactcaa aatctttcat
    16321 tccaatttcc acatatgaat atcgaaagaa aaactggaaa tatatatgct gacagtcaaa
    16381 gaaatgatga atctattaag tatgaattga tcgatggcgg tagaaaatta tatgtgccta
    16441 tttggtgtga tttgggaaat tattctattg ctatcaaaag tagtaacttt ggtggtaact
    16501 atatctcgtt ggatatgaac cagaacatag atgtttatgc ttatatgtat gcaacgatag
    16561 atagtgaaac aaaaaaagat gatgaattac tgcttgaacc tgtttatcca gagtctactc
    16621 taccagatgg ttggagcaaa aatgagattg attggctgaa taaataagtt tgaaaagttt
    16681 ttatctatct gatagaatta aataatgata tagggatggt gatagaatga gggttcaaag
    16741 ctatggtaac agtgtctttg atgttaaaag agaatttcaa gattctagaa gaaaaaccaa
    16801 aaagctgata gcttacttca ttctatcagc tatcgttttc tatattgtca agtttgtttt
    16861 ggaattaaac tcaatagtat tcacagatat ggttcataaa atgttaaatg gagaacctat
    16921 taatttcaac tttgattaca tgcttgaaaa agcacaaagc aatcaaattg tggcttttat
    16981 cgtgagtatc tattatttta ttgaaaaatc tattttttct ttaaaagatt ttttctttga
    17041 aatgcttacc aaactaacaa cgttttacaa taaaatttca aaaaattaag gaggaagttt
    17101 gaatgttaga tcaaaataca gacagaagtt ggtggatgat tggtgccgtg atcgtaggtg
    17161 ccatcttaat cgggacagct aaagtagctt ttcccgaagt gtttaatatc gtcattacat
    17221 ttttcaaaaa catgatccct acaaacttag gatagttcca ctaaaaaatt cacgtagttt
    17281 gatgcgtaga aaaataataa agaatgaaaa aggctacata aagtgattta tgtagccttt
    17341 tttttggatg gaagtgatgt aatgctaaga gatatgccca aaatgattct tttaatgttt
    17401 gttgggttct ttttaatttt agtgattgcg tactattcat taaatggcta tacacaagat
    17461 tcgtctgtga aaggtatgca agaaacgtta agaactacag caatagcaaa cagggatgat
    17521 agtattcgag tagaaaaagg acgatttgct ttaattaagt ctgattttga aaaagatttt
    17581 aaagaaaaat ttgggaaaag taaaaatcta aataatgaaa ccgctgatta taaatttgat
    17641 tatcttacag atgggaagga cggtattaaa gcaattaaag tgaaggttga aagtggatca
    17701 aaagaatatc aagcaacttg tattttaaat gttgcaaatg attagaggtg tgaaatttgc
    17761 gtgttgtaga ggaaaagaag atttctccaa aaatcgagga aactcctaaa agtaaaatta
    17821 atatcgcaag tattattaaa aaaaatggaa ttaaagaaaa ctctgaagcg atagaaggaa
    17881 accttgattt tttaactaag cttttagaag aagatagtgg aaattatgaa ccaccaattg
    17941 aagatgcaaa attaaatatg attcgtgatg atttaaccag aaactattcc caagaagttt
    18001 tacaagcttt aactgatgat gaaattaggc gaaaacttgt acaagttgtt ttgaaagaac
    18061 atggtacttt ctttaaaaaa gataaagagg ttgcagaata tgtagtacgt gaatgtattg
    18121 gaacagggat tattgagcga attatgacca acgatgaaat tacagatgtt ggttggaatg
    18181 gctctcaact tagcattgaa actaactcta acaaatggtt gtttgacggt gataaattaa
    18241 atatcacgca agagtatatt gaacgtgtta tttctaaata tgctgttgta aatgatcggg
    18301 cttttaatag tggtaatcct atcatggatg gaatgtttaa aaatgttcgt atatcagcta
    18361 cacacataga taattcacca gatggctcta cgttttctat gcgtgtggca aggcctagat
    18421 tagcattgaa taaagaaaac tttgaagatt ttgctcctga ttatattttg gagctatttg
    18481 agaaaatgat gaaaacaaaa gcaaatatct gtatatcagg ggagacaggt acaggtaaaa
    18541 cggaacttca aaaattactg ctaagtttta ttgataatca tgatcgtatg attatgattg
    18601 aggacgttca agaaactcac gctaaagaat tatttccgga taaagatatt ttctcttgga
    18661 tcactactcc caaaaaagca atttcagatt tagtaaaagc ttctcttaga aataatccac
    18721 gatggatcat tgtttcagaa acacgtggtt ctgaagctta tgaaatgctt caagcggttt
    18781 tgtcaggtca ctatgttgta acgacacttc atgcggttaa tgcacgtgcg atacctagac
    18841 gttttgtaaa tatgtgtgca agtggtttta aaataaacga aaatgcggtt gaggaagaca
    18901 taaatcgtta tttcgatttt ggtgtccata tcaaaaaaac agtggttgat ggtaaagtga
    18961 tccgttattt gaatgaaatt gttgaatttt ctgctagtga accgactaca gtttttaagc
    19021 aaaaatttat caatggaaag tttgtcattg aaaatggtag tctttccgaa gattttaaag
    19081 aaaaaatggc tgaaaaattt attacgtttg aatttcctga agatcaaact aatacagaag
    19141 aatcacttcc tgaagaagaa gaggtgattt aagttgaaag tacaggctat gacaaagcga
    19201 aaaggtctca tggatgtttc ggaactaaac gcctatcaag tagcgtatgg taaaccgtta
    19261 gattatagag attatttgaa ctatgttgga ttaccttgtt tgtttatggg tggcttttct
    19321 tttgctttat gttactactg gtggattgct ttgatttgtg gagtaattgg aggcgtatat
    19381 ggctttaaag ttataatgcc taaaagtatt aaacgctctt atgaattaaa gagtttacag
    19441 gaaagaaata aatttattaa caacatgact caaattttga ccgatgatag taaaacagtt
    19501 actagagcgt tatctatagc aaaacagcga acaaaaggtg aattaagaga agatattcaa
    19561 atcttagaat cacgtttaca aggtgctgat aaatttcaga ttgctgaagc ttttcgtgaa
    19621 atcaacaata aatattatag tgatgttgtt tttacacaat actttgagca attggaaaca
    19681 gctatttacg aaggtagaaa taattcagat actatgaagc aaataaagag ctatcataat
    19741 gatgtgaaac gtaaaacaga aggttttatg aaaatcaaag acggctatct tcaagatatg
    19801 aagcaaatga tgtttattgt agctgttttt attggagcta taacttttgc ttttggtttt
    19861 tctactttct atgaaggttt tgctcgatca cctatcggat gggtatttgg atctatttac
    19921 tttttgatta tggttagctt catgaagtca tttttcaaac attattttga tgatgaaata
    19981 atgagtttgg gggtaagtaa ttaatgaaag ttaaggcttt aaaaaagaaa agaaaatcaa
    20041 gtattagccc cttgaaaatt acgaaaacag aacataaaag cttttggatt aaattactag
    20101 gtgatgttta ttatcaaaaa ttacgtgaaa tgaacaattc caatattgcg attattaagt
    20161 ggcaaaaaca acggttaaaa aagtcattga tttttatcct agtcggattc ttaatgtatc
    20221 tatttttagg gaaagaatgg tattacttag ccggtggaat tgctacttca gtaggttttt
    20281 atgtttggca agcaaaatca atcaatggta tctataatca atttcggttt gaacgacact
    20341 tgcaatttag taaatttaca cggctattga tcccctatct gaaacaaaaa aaagatggcg
    20401 gaaatttata tggtgttttt gcaaaaatcg taaaaagact agattatgat accgataaaa
    20461 agctattaat gaagcttatg caagaaatga cagatcagcc taacgatatt agacctttta
    20521 cggaatatgc agaacagact tctggaacag acatgtccgt attatttatg tctacagttt
    20581 atgatattcg acaaggttcg gcagatttgg atgttattga agagttggat cgtttagctt
    20641 ctgaagagtt aatggccgga atcgattcaa tcattgacta taagtcgagg cgttttgtct
    20701 tctttcctac aaaaattgca atgacttcat ttattttagt tgtcggtttt gcagtaggcg
    20761 ttcttttcat gaacctaaaa gatattaact ttgggcaata aaggaaagga gaaagtcaat
    20821 gcaagaacaa tccgttatgg aaatcattaa atggtttata ggtttaatga tgattatggc
    20881 aatggtttct ataggacttt tttgtgttca attacaagat attaatactt tcaagcaaca
    20941 agtaaattat caaattgaac gaaaaggagg tctgacgaca gaagctgttg aagcaatcga
    21001 caaatattcg gctactaatt atgatagacg ttttacaatt gaaagcgata agttaaatca
    21061 gaaagtatct tatggagaag aaattgatta tacgatcaaa gcaactattc caattcaaat
    21121 tttaccgctt cctgatgtaa ctttatcgtt taacggaaat agtgtttctc aaataagatg
    21181 agaggaagga taaaatgcaa gagaataatg tttccagaat aatttatttg attggcggat
    21241 tgataatttt atccttagct attgccggtc ttgtagagca ttggccggaa ataaagttat
    21301 taataagaaa cttttacgac aataaaatga ttaaaatgta ggaggtatag atttggaagc
    21361 aattcaagaa aatcgtaagg ataatcaaga aacaagtgaa aaaaacaaaa aaaaaagaaa
    21421 aaagaaagta gataaattta cggaaatgac cgataaaatc attgataaga aaggacttaa
    21481 acctaaagac tttgaccgtc aagcaatcaa agaagcacga caaaaactgt atgatgattt
    21541 tgttaaagaa aacatatcac agcttttaga ctaacattta gggggaaata aaatgaaaaa
    21601 tttagttatc agcaagtttg caatgttacc aagaatggat tttttcgata atgtaagtaa
    21661 gtttatgtca aatatgactt caaaactaca aggaatttca ctatctgtaa ttatcttttg
    21721 tattgtggtt actggtttga tgtttctttt cggagaaggg ccaagtcgta ctgctaaaaa
    21781 atggttaact tatatcgtga ttggggcaat aattatcttc ggagcttcaa ctatcggaca
    21841 aacgatcaag tccgttggtg gtttctaagg ggaagcttct tagaactaaa aaattcgcaa
    21901 gtattgcatg aaagagcatt gagtgattaa tgctcttttt tattttgaaa aactataaac
    21961 taaggagatt taaactatga accaacgatt cgaatataat tttgaagatg tagaacctaa
    22021 gttactgaaa aaaggaaaag gattaaaaaa gaaaaataaa tatactctca aacaagcaga
    22081 agcattagta gtatggattc aacacaattg gtcagacaag acaacaatta ccttgatctg
    22141 ttcagacaca gaagaaggaa caaagtatgc ttttgatgga attgaaatta ctccattttc
    22201 gattggcgga caagaaaaat tattgtttcc tgatattgaa ggacaaattt tacaagcgcc
    22261 ggacagcatg atttccgatc ctaataaact gattttggat attaacgatc aatacaaaca
    22321 cgaaccagac gtagcagaac caacaaaaga atcaaagtca aaattaaatt tgaagctccc
    22381 ttcagtagta ttaccatttg ctaagaagaa aaataaactt atgaacgaat tgattgaacc
    22441 agaagaaacg gatatttatg aagaagaatt agaagaacaa gaagagatcg aagaattagt
    22501 tgtgtcttct gaagaaacag aagaagtaga agaaattgaa actgtttctg aaaatgaaga
    22561 agaaacagaa gaaatctttg actttgaaga tgaagaagaa gtggaaaatg tacagaaagg
    22621 cgatctatct acaagtagtc aaaatgggtt ggaagctgta aatgatccac aagttccacc
    22681 atatatatct tcacaaactc ccaattactc tccacagtat caagcgccta atgtcttaca
    22741 accattaaga aaacacgaaa cagtgcagtt agttacgctt aaagagtact gtgatttggg
    22801 agaagaaatt gaaacttcaa tttctcaaat ggtcgaaaag ttgaaaccag aatctttatt
    22861 caaattcata gggattcctt ctacaggtct tacaaatact cgtattgatg aatatagaag
    22921 taaccatgct atgttgcgat tagctgaagt aaaatttgaa aatttaagag agcattattc
    22981 acggcaaatt atttctttta aagcagaagc cctagatcgt ttaagaactg cggtagatat
    23041 tgcttggaag aagccttacg atttagatgt gaagaaaaac aaggccgaag agttatcaaa
    23101 aatggaagca gaagcagaag ctaaattaaa tgagttcgtg aaacgtcagg aaaacaatgt
    23161 aaatgataaa atcaaaaagt ttgagatcga acaagaaata gatttaaaaa actttattgc
    23221 aagacaagag gcagataaag cggtatttat tgctacagag aaagatcgga ctcaaaattt
    23281 aattgattct aaagcggaat caatcaatgc agatttactt gaaacaaaag ataaatttat
    23341 tgatgaagaa atgtacaaaa tgaaaattga aatgaacaaa aatttatttg atggcaagca
    23401 aaaaattaaa tcagaattgg ctactgaact cgctaaggca aatgataatg tttgggaaaa
    23461 tgcgctgaaa tttattcaag aaattcaaga tgaaattgaa gctaaaacac caatttgggc
    23521 tgctgaaatc aaggagttta atagtttaga agaacaagaa catgaacgta tgaagcaaac
    23581 taaagaactt caattaaaag aggaaagtat tgaaatcaag cgtttggaag ctgaaatgaa
    23641 tttgaagcgt atggaagaat tagatcatga aaataaaatg cttaaaatta aacttgaaac
    23701 tgccctttca aaaaatgagt tattagctat ggaacaagat acaaagcaag tggtgaatgg
    23761 atcaaacaga gttggtgcac ttaatttatt tagtaaacgt taaaggagaa cggatatgag
    23821 tgtttttaat aataaagtta tgttagggaa ctgttatgtg aaccacattg gtttttacga
    23881 tggaggaaca tacagagttt tattcttacg tttatctgtt tttagttact caaaagatgg
    23941 aaaggggcat tatgaaaata ttaatgcaca aatttcttgt cctttagata gaaatgggga
    24001 aaccacgact acaagtagtc accctaaagc cttgatgaat aaatttatct ttgaaaagtt
    24061 ggatcgcaat ttggttgagg ggatgaagat aagtattcat ggaaatattc gtggaacaga
    24121 acaagtgctt cttaacagtg gttggaaaaa ctttggtcaa ttatcgcctt tagaaaaaga
    24181 agaatatcaa aaaattccta aaaatgaatt agtaacaaga aatcaaacat atatctatat
    24241 agaagaggta agtatgccag aaaaagaaga atttagaaag tctcaatcta aggtaacaaa
    24301 agaagatgtg gaagttgcag aaccgaatca agcagatgaa attttatccg atgatactga
    24361 tccagtgaat gatgaaggag aggttattga agtttttgaa gctgaaatta tcgaagagga
    24421 agaagaaatt aaaagttttg ataaaagaat gtatcgagag aaaccaaaag gttatgtgaa
    24481 ttttgaagat atgtattatg acgaatattc taatgaacca ccgatcaatt agtgggggtc
    24541 aaatatgaag ctagtacaaa acaatgaagt gaaagaaata aaaaacggct ttgttaaaaa
    24601 ctcgaacaaa attttgggaa agctgaaaaa agtaggtagt ttcaaaaaaa ataaatatca
    24661 tactggaaaa attgagggag aagatttacc aaaaagttta caagataaat tgaatgttcc
    24721 gcttgtattt ataggtgttt ttttaggttc aatagttttt ctagcaataa cgtacttatt
    24781 gaacttttta aaagctttgt tagttgcaat cggacagtca gaaagtgggc tacttggtgt
    24841 agagttcgat cctaagttta gtttggcata tctaaaacca cttggggata cgaaacaata
    24901 ctttatgtgt gctgtggttg cggtaatagg agtcgcttat ttaatatatg ggaaattaaa
    24961 tcatgaaagt gagaaaaatc ttgtttacgg tcaaaaagga gattcaagac tttcaactat
    25021 caaagaaatt aagagagatt accatgagat accagaaaaa acagaagtgt atgatggtat
    25081 tggtggtgtg ccaattagtc actatcaaga taaatattat attgatagag acacagtaaa
    25141 taccgttgtt ttgggagctt cacgatccgg taaaggggaa acatttgttg ttcctttatt
    25201 agataatctt agccgagcaa aaatacaaag taatatggtc gtgaatgatc cgaaaggaga
    25261 attgtttagc gcttcaaagg aaacacttga aaaacgaggt tatcgtgtat tagtgctaaa
    25321 tattgatgat ccacttgaat caatgagttt taatccttta caactagtaa ttgatagttg
    25381 ggctaacaag gactatcacg aagctagtaa gcgagcgaat actttaactt caatgctctt
    25441 tgcttcagga atgggtacag ataatgagtt tttctataaa tcagctaaat cagcggtcaa
    25501 tgcgattatt ttaacgattg tagaacactg ttttaacaat gattgcattg aaaaaattac
    25561 aatgtacaac gtggctcaaa tgctgaacga attaggaagc ttattttaca ctgatccaaa
    25621 gactggaaaa gaaaaaaatg ctttagatga atatttcaat actttaccac aagggaatca
    25681 ggccaagatt cagtatggtt ctacttcatt tgccggtgat aaagccaaag gttcgatttt
    25741 atctactgct tcacaaggta ttgaaatgtt tacaagtgat ttgttcggga agttaacaag
    25801 taaaatgtct attgatttga aagagatagg ctttcctaag tccatacaat ttaaagtcaa
    25861 tacgaattta gttggaaaga ggattagtat ttcatttttg agaaaagaaa atgatgtaat
    25921 tagattaatt aagaagtatc gagtgaaagt aaaagcttta gggctatgtg ttttaaattt
    25981 taatgaatat cttcaggatg gagatatgtt ggatatacga tatgaagaag agaaatcgag
    26041 agcatggtat cgtattgaat ttcccaaaaa acaagcaaat agtcatgata cttctttagt
    26101 cactaaaaaa ttcaagtcag gtaaaaatta ttctgactta gaaatcagca ccttacgatt
    26161 aaaatacacg gatcagccaa ctgctgtgtt catggttatt ccagattatg atccgtcaaa
    26221 tcacgtactt gcaagtattt ttatcagtca actttataca gaattagcaa gtaattgtaa
    26281 gcaaacaccg gataaaaaat gttttaggcg agttcatttc ttgttagatg aatttgggaa
    26341 tatgccggct atagacaata tggatgggat tatgacggta tgtctaggaa gaaatatgct
    26401 ttttgactta gtgattcaat cctatagtca gctagaaaca agatatgata aagcttttaa
    26461 aacaattaaa gaaaattgtc agaatcatat tttaatcatg tcgaacgatg aggaaacgat
    26521 agaagagatt tcaaaaaaat gtggtcataa gacaacaata aatagaagtg ctagttctaa
    26581 acatttagat actgatagct cggtcactag ttcggcagag caagagcgag taattacagt
    26641 tgagcgtgct agtcaattga ttgaagggga acaaattatt ttaagaaacc ttcatagaca
    26701 agatacaaaa agaaataaga ttagaccatt tcctattttt aatactaaag aaacaaatat
    26761 gccgtataga taccaatttt tagcagaaga ctttgatact caaagagata ttaatgagat
    26821 tgatattgca tgtgaacata tcaatctatc tttacaagat aaccaaattc catatgcaag
    26881 gtttatcaaa gatttaaaaa cacgtttgga gtattcaatt attaataata ttcctattag
    26941 tgaagaagac tatcaggatt atctaaattt atcaggatca caacaaacaa ttaatgaaga
    27001 agagctgtta aaactggtaa atacggaagt aaaaagtcaa gatatggaag gtcaagtttt
    27061 atcagaacaa gaaatgaaaa ttaatattta tctaaaggaa agtcaaaagg aagcaataga
    27121 attagtaaaa gtaattaatg gaaggttagc tgatgaaaat tcagcagata tggttataaa
    27181 aaccattaat tcacaaatcc ttgatacgcc tttcattcaa tctttaatca atgattctat
    27241 agctattcat aatgttcaag agagtaagga tcaaattatg atcctagaac aacaattagc
    27301 taatgcttat gagttagcag tgtttaaaac gaaaaattca atactgaaaa ataaaattat
    27361 caagttgaaa aatgctatga ctaagctagc aaaagcaatt tagagagaag ggaagaaata
    27421 ttgactgaat ctattaattg gagaaaacta aatgtgcaat ggttaaagta taaatggcca
    27481 atcatagttt ccctaataat cattctattt atggcctatc cgcaacgagt ttttgcgttg
    27541 ccgtggatta cagacaataa agaggtatta aaatttttaa caaaatatca agattatctt
    27601 cagtttacta aaggtggttt tggaagcatt gtcgttcaaa gtttatcgtg ggggctgatt
    27661 agaggtgcat attttgtttg taaatcagca gaaaaccttg ttccacaatc gctaagtttg
    27721 tttagctttg ttgaaagcag tagtttaggt gctgtttatc aatctgtatt aaacacggtg
    27781 gttgtaggat taatgatcct ttcattgatg tttgttggtt acaagatgat tactggaaaa
    27841 ggaacagtgg atttaaaatc ggttggaatg aacgtagtga tgtctatagc attaatactt
    27901 ttaatgccaa ccatgatttc tagtggtatt caatttgcta aaacatttta taaggactct
    27961 actacgatta caaaagctga tgggggcgtt gcttggtcat tgattgagga cggtgtaact
    28021 gatttagtat atatcaataa aaacgatgct tatgatcggt tgggtaaaga tggcgtacaa
    28081 aaaaatgcgt taaaagaaaa gaatttcttt tcctacgatt tcagtgaagt aatcacagtt
    28141 gatacagcta aagatttaga agatgataat aaaaaagctg aatatcttca atctaggttg
    28201 acaccagatg gcgaaggaaa tatgcaagcc gttaagattg atgatggttg gatgtcgatg
    28261 ttctctgata gtttcaaagg agggtactac aggtttacta aaaatacttt tgcattgact
    28321 ttaggattac tttcgttagc tgtagcctat gtttttagta tttttatcat agtcaccgct
    28381 attttagaac tggcatttaa gcgtgtgtta ggggttcttg tttttgcaac agatattgag
    28441 acaggacagc gttcaaaagt tgttctatca gatattctac agtgctattt gactgtaggc
    28501 tttcaaggat ttggattatc catgtttgca atgtttgttt cctatttagg aacaggcgga
    28561 gggtcttccg ttaatccgtt tattaaggct gtagcttatg tatgtgccgt ttttgtttta
    28621 atcaaaggca gtggaacagt catgagatac tttggtgttg atattggact caaagaaggc
    28681 tatgggcaac ttgcttctgc ttttggaatg ggtgcaatgc tgtttagaaa aggatcaaat
    28741 ggatttaatc gtgccaaagg aagcggaaac ggaaatggat cagacagcgg agaaggagaa
    28801 gatcgaaaac cagaaaagaa tttcggtgaa actctatcaa aaaaagctgg caaaactgga
    28861 agagctttgg gatatgccca tgaacgtgga ttatcaggtt tagcttcaga tggagctaca
    28921 atggctagtg aaagagcaac aaaaccattt aagagtatgc gtgatatggc taatgatact
    28981 aagaataagt tcaaagaagg tttagatgat ggtacagtgt ccgctattaa taaaaatagc
    29041 aaacctatgt tagctaaaaa taaagaagat gaaactggaa aatacgctga ttccatgcca
    29101 acacgtcttt cggatagaaa agatggtgct aaggttgaaa atgccgatag aattatgtct
    29161 agttctgaac gtatgcgtga agcaatgaaa aataatgctg aatcgaataa taatcctgta
    29221 agtgcaattc agcaaaaagt tcaacaagat attgaagaac gtaaaaatgc tatgcacggt
    29281 caagctaagt ctgctgaaga attaattaat cagaagcgac aagaagctaa atacacgcct
    29341 gaagcaatga atcgtgagga aatgttaagg aaacgtgtag aagggcgtac aggaactaat
    29401 acggacgaaa aagaagcgtt gaccaaagaa agaatccaag aagctaaaaa ctcaaatacc
    29461 ggattagaaa aattggtcaa agaaaatcta caaaattcta caagtagtca aggtggtcga
    29521 tcagtagatg tccgagaaaa tattcaaggt tcattgaatg gtcatgtcgg acggaatgtt
    29581 gatgtaagag agaatttaca aagttcttca aatggtcaag gtactaagac aattggtgtt
    29641 cgagaaaatc tgcaaagtag cagtacaagt caaccaaaaa cagtagatgt tcgagaaaat
    29701 gtacaaaatt ctacaagtag tcaagggttt aaatcagtgg atgttcgaga gaatgtgcaa
    29761 ggtagcagtg caagtcaacc aaaaacagta gatgtccgag aaaatgtaca aagttctaca
    29821 agtagtcaag gattcaaaac agtagatgtt cgggagaacg ttcaaaaaga tagtggagaa
    29881 atgaaagaaa aaacacaaaa aatcaatatc gttgaagaca gaaagtcagc gaaaaaattt
    29941 gattcaaatc atgaaacagt gattattgat agtgaaatcc gtgaaaatga taagggatca
    30001 aaaccaagac gaagattcac ttatgaagac aacgaattat tcagagatac acttaatgat
    30061 cctaatccac ttttcgataa tttaaattaa attaaaagcc ggcatgagtg attatgccgg
    30121 ctttttatga ggtgaaaatg atgtatagaa atcccaaaaa tataaaacaa gagataaagc
    30181 tatttttctt ctatttagtc gatttaggaa tagtagccgg aagcgtgatg gcaagtgttt
    30241 actttgcaaa attaatgcca atttcaccaa tgatgaaatt tgcactgtac ttattagcat
    30301 ttgtagtggg tgtttggatg tgcgttagaa cgcctaatca ccctaaagag cgaaatattt
    30361 taatgctgta ttatctcttt aaaatggata gaaacagata ccacgaagta gagtttaaaa
    30421 gaaatagtag ggggaaaatt gaattatgat tgttgctgaa agtaatgaaa ataaattaag
    30481 tggtgttttt gacaaattct tagaactagt tattccaaaa gataaagata tgtgtgaact
    30541 tttaccatat aaaaaaatat tgaaagacgg aacgttagtg actaatgaca ataagtatca
    30601 agcctttttt aaagttaaaa caactgactt aacttctatt tcagaagatg gatttatgcg
    30661 tatggtaaac aaactcactt atttaaatcg agtatattta gaaccatata aaatttattc
    30721 attaacttat cctactgaaa cagtaaatca agcgatttat tggaagcata gaatacagga
    30781 ggcaaagaga agtttattga gtaataatga tcccaattta gaaacgtatc tgaagtggaa
    30841 tctaaagcta gcttacgata attatgaaag agttgatcta gtatcgactt taccagagtt
    30901 gatgttcgtt atttgtgttt atggaaaaac acaaaaagaa gtgaaaaatc atgccgatgt
    30961 aatgaaacag gccggtggta gagaattaga tttacaaaga attactgaag gggatttaat
    31021 caatgtgata actaaattga tgaatcccaa tacttcttta taaggcggtg cttattattt
    31081 gttaaagcac tgtagaaaag gattttgtaa agtaattaaa ggtaaaaatg ctgctgattt
    31141 aatctttgaa tggaaagaat ctaatttaat taatgtacgt gggaaaaatg gaagcggaaa
    31201 gacagaaaga gtgatggcta gtattgataa ttcaaggcct agatcaagag cattagtaat
    31261 aactgatgaa aaaaatcact ataaaggcat acgaacgata gaaaaaaaat atttaactaa
    31321 caatgattta aaagtagtag atgatatttt aaatatttat cctgatttta atttagttgt
    31381 ttttgatgaa acaattcctg tgacagaaat aaaaggattc tatcaaagct tttccaatct
    31441 agcaaataaa tatgaggaag ttctgtttgt ttggattaca gaagaagata gtaatagcaa
    31501 gtcagcgttc tttagtgaaa agaacgcttt tttacattct atttaaaaag gagaattgaa
    31561 catgattgta agtaaaaaac tagcaagaaa tctagtaagt gaagggtatg acttaagatt
    31621 agtagaaaag tatcaaccga gcggtggaat taaatttgat gagcgttata tttctactgg
    31681 tgatggatat gtaacttcga ttgctgttat tgagtttgca gaagatgtaa atagtcgatg
    31741 gttaacggat ataatggcaa ttccaaatac tattgcaact ttcgatgtat gtacggcgga
    31801 taaagaagac ttattacaca aaatcaatac gtctattgga gaattagatt accgatcaac
    31861 aaatgagaat agaacaactg acaaaacaga cgctttacta gataaataca atttagaaga
    31921 ttttgcacaa gaaattacga aacgtggtga agttgtcaaa actgttcatg ctcgtatttt
    31981 tgtttatagc gattctttag aagaattaga gaaaagaatt agtgagattc gtaaagattt
    32041 ggctagtgtc aatcacaagg caattgttcc gcaatttaga atgaaggatg aatggatgtc
    32101 tttatttcaa gatggcatgg aacaagaatc aaaattattt gccaagaaag gcttaactgt
    32161 tccggcaaaa aatattggtg gcggtattcc atttcaccac aagagtttga aagatccacg
    32221 tgggttttat ttaggaacta cgtcaactgg tggtgctttt atatttgatc cattttattc
    32281 tacgaagcaa agacggtcgt ttaacggctt tattttagga aagatgggtt ttggtaagtc
    32341 aactttaatt aaacagctac aggaaggcct acaagcgaaa aattgtttta ttcgaggatt
    32401 tgacaagtca ggtgaatttg aaggcatggt gaagaaagca ggcggtgtat acgtcgattt
    32461 aggcggtggt gatatggtag taaatccatt gcaaatttat gcaacagtca tgaaaaaaga
    32521 tcaatttgga aatgatgttg tagatgaatc aaagagtttt caaaatcata tcactaagtg
    32581 tagtaatcaa attcgctttt tagaacctga aattgatacg gcaactgtac gtgaatatac
    32641 tttgatgtta caagactttt atattcatta tggaattttg ccaaaagaat ggagagaagc
    32701 gagagacgac aacgaacgaa atcaattaaa tacaaatatt tgtaacttgc ctaatgattc
    32761 atatccaatc tatggagagt ttcttaaatt tattgaaaat caacaattca aaattaaaaa
    32821 tgctactcct gaacgtgtcc gttctattga gctaattaag cttatcgtta cggatatgtg
    32881 tacaacttat ccgcaaattt tcaacggagt aacaacaatg ccaaactttg atacaacaaa
    32941 aattgtttac tataacattg attctattag taaaatgagt gccccagtat tccattgcca
    33001 attatttaca gcgttaacga ctatttggaa cagtgccttg caaaacggaa agattcaaaa
    33061 agaaaacttt aaaaaatatg gcagtgatta ttataagtat gcacaagatt tcatggtttt
    33121 gatcgatgag tgtcacaatg ttatcaattc aaataacaag tatgcttgtg aatatgtact
    33181 aagttttgag cgagaaatgc gaaaaatgcg tgcaggtgtt tattttgcaa ctcaaacgcc
    33241 tgaagaaatt atccctaaag attctggatc agaagtagca gatgtgatta gaaacatttt
    33301 taatctatgt acttttaaat gcttctttaa tttagattct gcattgttgg aagacataaa
    33361 aaaagtttta ggaaatacaa ttactgatac agaaattatg cttttaccag aattagaagt
    33421 aggacaagct gtagttcaaa cttccagtga agacacctat atgattaatt ttgatccata
    33481 tccagagcaa attgaacgat tcgatggtgg tcaataatag aaatcggagg tgaatttaat
    33541 ggagcagaac aaaaataata atgttgcttc aacactcgct cgtcaaggga tgaagcaagt
    33601 atcaaagaaa ataggtcttg ctttaaaaat aaaaataggg gtagttatag gactgataac
    33661 attggttggc cttttagtta taggttcttt agctacatta caaggaacac aaactgcaac
    33721 taatacagga ggtgcagaag tcattggtgg caacaaaccg ttgtcacctg aagttcaaaa
    33781 atggcaaaaa acggtagaaa aatatgctga aaaatacgga attaaagaat tagtacctta
    33841 tgctttagca attatgcaat gtgaatccgg aggtaaattg ccggatttaa tgcaaagtag
    33901 cgaatcagcc ggattgcctg taaacacact tcaatatgaa gaaagtatcg atcaagggct
    33961 aaaacatttg agtaatgtgt ataacattgc taaaagttat ggcatggaaa gtaactatga
    34021 tggaatagtt caagcttaca attacggaat agcttacatg cactacttag gaaaaaacaa
    34081 aaaaacacat actatcgaaa cggctgaagc ctactctaaa aatgttgttg ctccaagttt
    34141 aggaaatact acaggacaga cttattcata tgtaaatgaa gtatcaatta aatatggaaa
    34201 aacatatttg tatttaaatg gtggtaattt ttactatgca gagttagtta aacagtatat
    34261 cggtgggggt agtggtaatc cggctaatcc ttcagaattt tggggaaaaa tgaaaacaga
    34321 aatggaaaaa tatcaaggtt ggccttatgt ttggggcggt aaaaatccaa cgacaggttt
    34381 tgattgtagc ggattaacag cttatcttta tcaatttagc gccggaatta caatagatag
    34441 ttatacagta acgcagtatg aagcaactga agcagtatca attaaagaag caaaagctgg
    34501 tgatctgatt ttctttagag gaacatatgg agcgcctgat ttcatttctc acgtaggtat
    34561 ttatgtcaat gaaacaacta tgtatgacag taattcaagc ggaattggtt atcacaattg
    34621 gcaagattca tattggcaat cacatagtcc agagattcgt agagtcaaaa aataggagga
    34681 agaaaataga tgattaagag aaaaaatgat aataaatgga aatatatttg tggtgtttta
    34741 attattattt tagttttagg tggattgtta tctcaacttc atacaagtaa cattcaagca
    34801 aaaaataaaa aattggatca agaagttaca caactagaag aacaaaatag caagttaagt
    34861 aaaaatatta gtgatctaaa tgcaaatgta gaagcgaact taactaatga tgatggcagt
    34921 gaattaaata aacaaattgc tgaagttgct aagaaatttg ttgagctata tccggtatat
    34981 gatattgaaa aagtagaaga aaagaaagct gatttagaga aaatagctac tccttctgta
    35041 gctgattcta tcgtgccgga cgacatgatt acaacgagta aaaagacttt agaaagtaaa
    35101 tctactactc aaaaaggaga agcatattca agtgatccaa cattcaaaag taaatataaa
    35161 gattcaaaat tatatacgaa ttatgttaac gccaatacta ttaattattt tgctgaagta
    35221 aattataaaa cagaatcttc tagtggagat acagaaaata cagtctatat gctttttgaa
    35281 gtaaccaata aagacggcca agtgttggta gataattatg aaatcaagta cttaaaatag
    35341 tgaggtgaag tatatgaata agaaatattc cattcataat aagaaggaac caaataaaaa
    35401 aaataaggca caaattgaca aagtatataa aaaaacgaaa ggagtaaaat tatatttagg
    35461 tgctatactt ggatttttag caataggaat gataatcgtt ttttctcctt atgtgatggg
    35521 gaaaagttat gattttgaaa ctgtaaaaat gagttcagcc caaaatgtaa cgtcttcttt
    35581 gaatatgtat gttgatgata tggaaataaa tagggaagcc ggactgttta aattagtatt
    35641 gcggtatcaa gatgatacag gtactaaatc actaagtaat attaaaagtg actataaatt
    35701 aaattttata acgaacaaaa ataatgaaga tgttaagaca aaagttgtaa aattgtcaga
    35761 tgaatacacg gtaatttatt atcaaaatct gcctaaagat tttggtgtaa tttctgtgac
    35821 agttaaccct agatatattt atcctgaatt agaaccttca aatgacttaa aagaaaagga
    35881 aataaaattc tatgcagtag acaaagatat taaatcaaat gctaaattgg aagttgaaag
    35941 tacttctgat ttgcaaaaag ataattttaa atatcagatt aaagctatag aaaattctat
    36001 aaaagatgag aaaaaatcaa ttgaaaaaat gaatttagca aataaagtag tgcaaaaaga
    36061 tattgataaa gctaatcaaa acttagaatt tcaaacccta gacgaacaaa cagatacaaa
    36121 aaatgaaatc agttctatgc agacgactat tgaaaataat aaatcttcaa tagaggaaag
    36181 tgaaaataaa atcaaacaat ttgaggaaaa aataaagcaa ctagaaaagt ctgcaagccg
    36241 atttgagtga tcgtgtttaa attattttaa gggagtttag agagaatgtg taattctatt
    36301 gtcactattc aagagaaagc tgaagaagaa ttaatgagaa ttgaagcgga atataaagaa
    36361 aagagaaaag cgattaagcg caaaaaaaat gcagagctta aaaaatataa agaggaacaa
    36421 gcgttaaaat taggaatgat gttgtgtaaa tattatgatt gctatgatat aaacgaagtg
    36481 ctaaaaaaag cgaatttaaa ccaattttgt gaatagaaaa gagagtggca aggtaaaact
    36541 tgccactctt tttttgggcg taaaagcttt agttactatg accaaaacta cggacgaaac
    36601 tatgaccaaa agtaggtact atattatgac cgaaaatagg tcgaaaatgg atttttttgt
    36661 gatagcaaaa aatatgaagc tattttgaaa gaaaaccagt tggcacagcc aacttagcca
    36721 ccttccttta ggaaggtgct atcagttttc ccttatgctc tttgtattaa ttccttggtc
    36781 ttgtctttaa atctttaatt ttcctttgga ctttgatata ataatatata ccagatagat
    36841 aaaaacagct tgaaagaggt gtgtgtatgg atgtttcaat cagaggaatt gatccacaat
    36901 atgtaaaaga gattgataaa aaagttaata aaattcaaga acgtaccgga aaaaagttta
    36961 gtaggaatga ctatttttta gcaatcattc gccaagatag cgagatcgat ttgattgact
    37021 ataaaaagtc agaatttgat ttagtagttg aaaaactttt attgtcaaat gaacaaatgg
    37081 tagaaagtat tagcgagtta acttcagttt atgaacgctt attcaatttt ttaattagcg
    37141 aaaggagtga tttaaataaa tgaactaccc ttatttcaaa gtatcagcaa cagaagatgt
    37201 aaaagaaaaa ttcaataagt gctttgaagt aaacaagtca gtatttcaaa caaaatccaa
    37261 tatgttccgt tatgttgttt caaatttacc aatgttaaca aaagccaatc aaaatttccg
    37321 tgatccagaa aaagaagagt tagctaaaaa cttagaagaa gcaaaattaa aaattagcga
    37381 acttgaaacg ataatagaat tgcaaaaaca gcaacttgaa aactttaata aaaaagaaga
    37441 cagtacacat aaagattata gtgaacaatt aaatagtata gaagagtctg tctataaact
    37501 attaattaat agtggattag ctagtaaaga attagaagaa gagcgtgctg aaccagaaca
    37561 agaaagtagt cgtttgggag agggaacaat gtttagttca ccgtcattaa gatttacaac
    37621 ggattgaaca ctaaaaaatt ctaatgcaac ttagcattaa gtacaaaacc gcctgtagaa
    37681 aaaatttttg aaaatggtgg gtgatgatat gactgaacat atctttaaca gaaaaagtgc
    37741 gagcattatt cttaaagcaa aatttgaaac tggaaaaagc aaaaagaata atcacatggt
    37801 agaacttcaa aaatttgtag attatatttc cagacaagag gctattaggc aagataaact
    37861 agatcatgat tattcagaag atgaactaaa tgaattagct agaatagaaa aagctttgga
    37921 aaaaatagaa caggaaacag atcaaccagt gattaaagat ttacgtgaaa tggataaata
    37981 tattgattat atgacacgta aaaaagctat cttagaaaat gtagataatg aaatagtaaa
    38041 cggagcattt agcaatacaa aaagatatat cactaaaaaa gacatttcaa aaataaaaga
    38101 atcggtcata gaagcaaaaa ataatggatc ggtgatgttc caagatgtaa tttcattcga
    38161 taatgatttt ttagtaaggg aaggatatta taatccagaa acaaatgaat taaatgaaaa
    38221 tgttctatat gaagcaacaa agtctatgat gggaaaaatg caagaaaagg aagaactagt
    38281 tgatcctttt tggtttgcaa cgattcacag aaatactgaa catatccata ttcatgtaac
    38341 agctatggag cgtaaaaata ctcgtgaaat aatggaatat gatggtgttt tacaagctag
    38401 gggtaagaga aaacagtcta cgttagacga tatgattttt aaattcggta gcaaaatatt
    38461 agatagaacg aacgagtttg aaaaaatttc taagttgaga aaagaagttc ctttagaact
    38521 taaacagtct gtaaaagatt ctttgattca attatatgta gaaaataatt caagatatga
    38581 caaagaatta gtaaagtacc taaaggaatt aaaaaaagaa atacctagta caacacgtgg
    38641 ctacaatgag cttcctgaag aaacaaaaga aaagattgat gaagtcacaa attatatgac
    38701 aaaggataat ccaaaaagaa aagaatatga tcgtatgact aaagaaattg atgagctata
    38761 tcaaacaacc tacggtaaac gctacaatcc acaagaatat tatttaaata gaaaaaaaga
    38821 ttttaatgct cgaatgggta atgctgtagt aggacaaatc aaacttttaa agcaatctga
    38881 agaaaaaaat ttgaaaagta atgaacttaa aaatctatta gacggaaaaa aattaaacgt
    38941 taagtttgat agagaaaaac cagaatttaa aacgaaatat gctaaacaaa gttatgatga
    39001 tttccaaaaa agattagaag aacgacaatt agcacgtgaa aaaaatgaag tccacttcaa
    39061 gaagatttct gaaaaattta aagataaaaa gcttattaga aaaattgata gagtaatcaa
    39121 tgatgattta aatatgtatc gtgcgaaaaa tgattatgaa gtagcgcaac gaatgataga
    39181 gcaagagaaa atgagacaag cacgtgaatt tgaaatagga atgaattagt agaaacggag
    39241 aatagaaatt atgaaaaact tcgatatagt gtgcagtaat acaaaaaata tatatttacg
    39301 tgagctactt aacagtgatt cggaaactat agaagatgta aaaaaaatca tagtgttgtt
    39361 tgaaaaagaa aacatggagc tagagaattg ggggcttttt gaaataccga ttagtgggaa
    39421 ttactgtttt tataattgga agacagaaga cgatgtagct tttgcaaatt acttttttga
    39481 taaaaattac ttttccccat tatatataga taagcatagt aatgaacaag ttgcttcttc
    39541 aataaaagaa gcaataaaat tggaaagagt gaggaaatga tatgatcgat tttagaacgg
    39601 ttatggataa actaagtaat ggagattttc gcgtattgat tgaactatac ttaaatgtaa
    39661 cgtctacagt caaagagttt ttatattcta atcccaaaac agctttagta ttagttgcag
    39721 tactgatagt ttttgctgtg atctctttta ttaagaaaat gattccgcta ttaattatag
    39781 gtgctgtgtt gatattaggt ttgtatttcc ctgaagtttt ggatcaaata aaaaatatat
    39841 tttaggctct aaaagcttcc agtagaaaat tttgggcttt gatatttgta ttaagcggtt
    39901 tgaaatagaa atttgataaa ctacatgaaa agacattata taagagtatc tgtgtattgg
    39961 aataaaaata tgataatata aaattatata tcagaccgat aaaaaagagg tgcttataaa
    40021 tgaaaaagtt aagagttatt gcaacaatac ctatgatttt tagttttgcg ctattattat
    40081 ctgcatgtgg caaagaagaa gctggtgcta agtcaaatga agcacataat atctcattca
    40141 tagatagtgc agaacaaaaa gactcacgtt gttggttggt agtcgaaaca agtagtaaaa
    40201 ataaaacgcc tattgataaa aatgaatatg taaaagcggt gatccaaact aaagatggca
    40261 aagcaaaagt atttaaaacg gatatggaac agcttacatt ggaaaaagtt tctaaaatga
    40321 gtgatgaaga aataatagaa caagcattga acgctgataa acaaaagttt aatgataaga
    40381 aaaaaagcga tattgatagc tataatgcca ctttagattc tgtaaaaaca gaattagaaa
    40441 atgaggaaaa acaagagttt caaaatgacc aaaaaattgc tgattttcaa gatagtatac
    40501 ctaaaataca ggcggatata gaagagatcg atggtgttca atattcagag ccggagtgga
    40561 aaatggtatc agcaacaatt aaaatggata atgaaaacaa atcaccttca atggaaaccg
    40621 ttaaattatc ttataaatat ttagatagtc caatttggaa tttaaatagt tttggtgaag
    40681 tttcgtctga aggagaatta tcttatgaat ctactagtat ggatttgaag ttatcaattc
    40741 cagttgccac aacgaaagtt ttagataatt tttattcagg atattctaat gatgaattgg
    40801 atcaactttt gcttactaaa gtagcttcta aagataacaa agttcaatta gatagtaaag
    40861 gtaaaaacgt aagtatagtt gagtagatgg taaaataaga agggataaga tggttactat
    40921 aactatctta tctctttttt atttgcatta agtcagtgaa attagataga agagttttta
    40981 tctatctggt ataatttaat taggtaattt aacttaccta aaaaagaaag gagatagaag
    41041 atggcctata aaaaaggaaa ctggaagccg aaaacgaaag agcaaaaaca aaaagagcta
    41101 gacgatttaa ttaaggcttc caacagcaaa atcgagcaat atcagtctaa ccctgaagat
    41161 atgatcgagt ttgccaagtt catgtctaaa atacacaatt acagtccttt aaacctatcg
    41221 ttgatcgatg agggatttga aggagcaatt gctgtagcaa gttatgatgg ttggaaaaaa
    41281 ctaggatttc aagttaaagc gaaagaaaaa gggataggca tttatgcgca tgctccggta
    41341 acggttttta ttgatgaaaa tggtgaagaa aagacgttaa aagaagcaac aagcgagcaa
    41401 aaagaaaaaa tcaaaaatgg agaattaaga agcaaaaaga tcaagcattt taagaagggt
    41461 tacgtatttg atatttccca aactaatgca actgaagctg atttgccaaa aatatttcct
    41521 aacagggtat ggaattttaa tattgatggt gaattaagtg ttagtcaatt agaaaaaggt
    41581 gtaaatcatt tggccgaatc tatgaatata tctataaaag atatgaacga gtctggtaga
    41641 ggtgaaatgg gaagcgctag aggtgaatat attcaatatg ttgatggtag ggaagaaatt
    41701 gtattaaatt caagaaatag cagaacacaa aatcttacta ccagtattca cgaattggct
    41761 cacaaaaagc ttcacaataa gaatgaacac ggttcttcct actcaacacc aattaaagag
    41821 tttcaagcgg aaatgacttc atttattgtt tgtcacaatt acggtattga tacgtcagac
    41881 tttactattc cttatattgc aaattggact aaaaataatg aaaaaattga tccaaaaaac
    41941 tttagacaga taatgcaaga agttagagaa acgtctattg actttataag tacgattgat
    42001 aagaaagtct tggaagaaca gcaaacagca tttaatgcac taaaaaattc ctttgttcct
    42061 aatgagaata aagagcagat acaacaaaaa tcagatttag aatttcataa tttctacgat
    42121 agtacaatca acgatccgga tagaacgatc cacggtagtt atttagaagc gatggtggat
    42181 tattatagag gaaagtatgg agaaaattct attgagctta ctcatagcaa ttatgatgtt
    42241 acagctaaaa taggagaaaa ctatgtgaca tattattcag atacgaaaca atttgatgta
    42301 tgtgatgttt atggtgtagt ttttgagaat ccaagtgaaa ataaaaatga tcgtttatta
    42361 ttagatcgat cacctgaaaa ttgtgattat gttgaaaagt ttgttgtaga aagtttgaaa
    42421 gcttgcgaga atcatgaact aaaaaatgat acgttaaaat taagcgaacc gctaagatca
    42481 gttgttataa atgaagcggt ttataatcta tctgaaaatg ctaacaatta ctataatgaa
    42541 aaatgttggc aagaagaaat tgaaaaccaa acaaatatta atgatgtaaa agaattaaac
    42601 tcttgtatga ctgcggaaga tttttacgaa tctcataaac aagaaatcga aaatacgatt
    42661 acagattttg aatctgtgtc aggaacaaat ttaagagata caatagacaa tgagtcatat
    42721 aaagaggtgg cttcagtggt agcttataaa ttggttgcaa ctaaagtaaa atatgaaatg
    42781 gatgaaggaa ctttcaagat accagaaaat tctaacttgg aaaagccgac tgtaaaaaca
    42841 gcagaacgtt cttttgaaat ggagtgaaaa aattatgagt aatgaacaaa attatgaaac
    42901 aggcggaact tatcgcctgt ttcatggaag tatgaataaa tttgatgaat ttagtttaga
    42961 tcataaaggt ttaaacggaa cagctaatgg atttggtgtg tacttaacac ctaataaaga
    43021 aatggctaca atgtacgctg aacagggtag taacggaaat aatggtttcg tatatgaagt
    43081 aaatgttgaa ttagagaagc cgatttctat gaatgaacgc actattactg atgaagagct
    43141 ttctaaagta atagataaat tacaagaaag taatgatatt ttaaatgatt acaatgatgt
    43201 tagttattac ggagaaaaag ttgtaaaaag agaagtgatt gcttctctta gtgagaatga
    43261 aaatgacgta gatttgatta acgacattgg aaatacaatt ggagatcatg aagctactgc
    43321 taaggcattt tatgacgttg ggaaatatac acatattatt gcggaagaac agttaagaca
    43381 aaaggatgta gttatcgtag tgttacagcc tgataaagta aagattgaaa ctattcacga
    43441 tttaaacgag gaacggaata gcttagaaag tagtgaggac atgacagata aatttgttgg
    43501 gcataaggtt tcggaagttc ccattcgaga agttgtgata aaagattttg agcaagagct
    43561 agaaacaact aattattatg acaaacaagc ttggcaagag cgcattgaat acttagaaaa
    43621 catggaagaa ccagtttcca gattagcatt agatagtgat attaatgaat tttatgatag
    43681 tcataaagaa gaaattgaaa cgaaattgac aaatttatcc gaaaagttag agatcgataa
    43741 agaagaactg ctaggttttc cagtagatac ttcttctacg gaatacaaaa aagctacgtc
    43801 tatcatggct tacaacggtg caatgagaga aatttataat gaaattgaaa atgatacgtt
    43861 tgaaatacca cctgtttctg aagaaatgga gaataagatt ttgaaattga ctaattttga
    43921 aaatacaaca aaatttgaaa tggagtagat aaatgaatga tagaacaaga aaatagaaat
    43981 tttttagaaa cgcaaggtga atatcgtcaa attaataata gtcattttgt aatttgtgaa
    44041 gatgtacaaa tcgcttttaa aatggctttg aagtacggaa aaagtattag aaaaaagata
    44101 attagagaaa aaaagagcaa gaagataggc ttttgggagt tatctcttag ggatgggcta
    44161 aactttattt tctgtacaga ggattattat gaaaaataca agtgtacctt atcgtaattt
    44221 tactagtgat ggtctagtaa agggggtttt tgtttggatt tgaaaaaggc aaaaaaagta
    44281 agtatcgttg atttttgcaa acaaaatggg ataggtttaa agcaagattc aagggaatac
    44341 tatagattag aagatcatga ttcttgtgta atcagtgatc gcaaaaacct ttttaaatgg
    44401 aactcaaagg atttaggggg agatattttt gattttatca aagcctacta tgattgttca
    44461 tttaaagaag caaaacagaa acttgaagat agcgattatg aagttcattc gttcaataca
    44521 aaagtgaaaa aagctccgta ccaatatcag aaagaaaatg aagtgccggt atctgttgca
    44581 aagtcttatt tagtaaatga gagaagaatc gatacagctt tagtggaaga ttttttatct
    44641 aaaggactaa ttaaacaaga taaaaaagga aatgttttgt tcttatggaa aaaagatcaa
    44701 gaaatcgttg ggtgtacgga acaaggaact agaaaatttt ttcatgaagg caaacaaaag
    44761 gatgttacat ggaaaaaaat acaagaaaac tctaaagagt attcaggatt taacgtaact
    44821 tttggtcaac cagataaaat gtattttttt gaaagtgaaa tagatttgat gtcgtacatt
    44881 acacaaaagc cagaaaaagc cgaaaatgct acgtttgtta gcatgaatgg attaaaggta
    44941 ggtaatgtat tgcatggggt tacagagcat tataataagt ttaaatcatt gccttcagaa
    45001 acagtccttt gtgttgataa tgataaagcc ggacgtactt ttattgaaac tgaatttaaa
    45061 gaaaaaggaa ttggaagaga agggatagga atcagtgaag taaaagtcga tgtacctttg
    45121 cgagagggat ttgattggaa tgataattta acaagaggaa aagaagcttt taaacgcttc
    45181 aagttagaag atgtatcaaa aatcgtgaac ttcacacata aattatctct taatactcct
    45241 gtatatgaaa tggaataaca agaagaaagt agttgatgtg ttatgaacga tacaatagca
    45301 atcttatcag attcattaat tgatttagat aagacgtcta cagactatat gattattgaa
    45361 gaacaactta aaaataaaaa tccagagttc gatattcaag aaaaatcatt gcgtattgaa
    45421 ttaccaaaat ttaaacaaaa aaaattaata actattctct ttgagattat tgaacgatat
    45481 gggcttactt attatcaaat ttggacggat aagaaagatg gccaatatac aaaaggggta
    45541 agcatagaag ccgttagaga agacgatcaa gtaagtatta aagcagttca acccttaatg
    45601 gggtataacg aagaattatt gaattgttta aacgtctagt gtaaactagg cgttttttta
    45661 cattatttgg ctacgaatag acagttaatt aaactaaaga aaactattga ctacaagtag
    45721 tcattcctgc atgatatagc catagactac aagtagtcaa agactcaaac ccttgattta
    45781 ataaaaagga gagataatag atggagcaga caagaatgtt ttggtcagat cgaatgcaag
    45841 atcacttgga ttgtatttac aaaaccagag agcaattaaa taagttagat actgtcagat
    45901 attattttac ctacttcagt gagaatgacg aacaaagatg ggatattttt catgatggta
    45961 attacgaaga atttttttac actgaaagtt aattatttgt tgaaatgatg aaggattcta
    46021 tttgatgaaa gggaacggaa aaccgttccc ctaaaaaata tccaaatgac tacaagtagt
    46081 cgtggaagga gagcggaata atggtaaata aaaaagaaac gtttaaagta ggtatggaag
    46141 tacagggcgt tgttattggg agcggagagc ttatcgaggg agaagtaatt agtttgttag
    46201 ctaatgtagc ggtagttaaa gcaaacggaa aagcaccggt agtgatgaaa aaatgcagca
    46261 tgatgtgtct tgatggttta aacaatccaa taactcaacg ctttgagaac gtgatataat
    46321 agaaaaaaga agattttcta ctgggagaga ttattatgaa agactatgct tcacgtaaat
    46381 ttatccactt agagcctatg aaagaattgg ttattaatgg gaatgaatat aatgattaca
    46441 aatctttatt tgtaaaggaa cagcctaaag gtggtattcg gatgaaaaat ttaccggact
    46501 tccgacttat tgaagtaaac aatttaggaa ttatagaaga ctatttagct aagttagatg
    46561 aagcggtaaa acaatcaatt gaaaaaaatg gtgaagacca tgtacttacg gttgttttaa
    46621 aagacttgaa agatagacta gaagctgaag cagagaactt taataaagac taataagaag
    46681 ggaatgagag atatgaccga ggctgtaaaa gaattaaaga aaatgtatcc tgatgttttg
    46741 aatatgactg tagatgattt tcacgaagcc ttaaaaaatg cagaaagtga ggaagagaga
    46801 actttctacc ttgtactctc tagttatgtg acacgagtag atcaaaaaaa agtaattaat
    46861 cagaaagatt ttaaaatatg attttagaaa atgataaacc atattatgta gttattgggg
    46921 gcgttaatgg cgccggtaaa tctacgattt atcaaattca aaattcaaaa agaaaagaac
    46981 ttgatactga tagaattaat gcagatgaaa ttttgaggcg ttttggagga aattggaata
    47041 aacaatcaga tataaacaaa agtatgcgtg aagttctgag agagatcgaa ggtcatttta
    47101 aagaaggaaa atcttttcac catgaaacga cttttggcgg taatccatat cccgataaaa
    47161 agaggcttct aaatgctaaa aaattaggtt atgaatcgtt tttaatctat ataggactag
    47221 attcaccaga aagagcgatt gaaagggtta aaagccgtgt tgctaaaggt ggccacggtg
    47281 tacctgaaga tttaataaga aaaagatacg aattttcttt aagaaactta gcagagttag
    47341 agaaatattt tgataatgta agtatctatg ataattcaga tgagttttct tatatttatg
    47401 ttaaagaaaa tcatacagtt gtagccaata atacgcaaga atatgcttgg gttcccaaag
    47461 ctattcaaga gaaaaatgct gaaactccta taagcaaaca aattatgatg gatatatctc
    47521 aagaagagat gaatccaaaa gaaacccttg aaagaatata ttcagcaagt gatgatttta
    47581 ttagtgatcg gttttcaaaa gaagcgcaag tcgatgattt ttataggaac cataaaaatg
    47641 aaatcaagga acggattcta tcaattagtt tttctacagg ggtatctcaa gatgaattag
    47701 taggtcaaga taaaagaaga aattcttgta aattggcata tattaatagt gtaggtgagc
    47761 ttttagtaaa aactgatagt gagctattaa gtatggaatt aaatagagat agaaatatcc
    47821 aaaaaactga ttttgaagtt gaataaaaga agttgtagct gaatcaatta atagtaaata
    47881 aatttaataa ggagtgcgaa agcacttctt attttttgga taaagtgact atatgtagtc
    47941 agaaggaata atatattatt tttagttttt gtttttaata taattactac aagtagacaa
    48001 ttgatcattg attatagtct aggaaatatg ttactattat tatataccag atatataata
    48061 atgaggtgct aaaattgaca ataataacta tgactaaaaa aatagaagta tatgttgatg
    48121 gatcacaaga ggaaagagat tcgttttttg gttttttttg gaaaaatagt agagaatatg
    48181 taaaagcgct gaatatggta tataaacagc tgatgtttga agaattgatc gaaaattcta
    48241 taaaagaatt ggatcatgag tatattaaaa gagagctaag tcttgaagag cgaattgata
    48301 atttactaag taatgaagtg atcgatcaaa agaaattaga taaaacgaaa agggaattat
    48361 ctaaactacg aattgcgaaa aacaaagaag ccagagacgt tttgacacaa gctataggtt
    48421 taaaaagtca aaccagaata cgtgatatag caaaaaaatt ggactttgat tattcggatt
    48481 ttatagattc agctaaaatg aaagctagta aggattttag caatgatttt gttggaattg
    48541 ttactgggca aagaacacct agaatctata gaaataaaaa taaatatgga ttgttaccgt
    48601 tcagaggtcg ggatattaag attcacaaag ctgacaagtt ttatatctct ttacctaaag
    48661 gatttaggtt taaagtaaat ttaggtagaa aaccaagaaa agccagagat agcgctttaa
    48721 ctttagaacg cattatggac ggtcaataca agttaaacca gagtaatcta atctttgagg
    48781 ggaaaaattt atttttgaat ttaacaattt cttttgaaaa agattatcat aagctagatt
    48841 tcaaagaagg agctgaatta accataatgt ttactgattt gtgtagcggt aaatgtttag
    48901 ttaacaatca atctgtgatt gattttggtg acagcaaata tataagtaac ttcaaaaata
    48961 aaattaaagc aatgaaatca agggagcaaa gccgaggagt tttttctaaa ggcggtcatg
    49021 gtcgaaaaag aaaattagat caagatagat gggaagccat aaagaaaaga gaaagcaatt
    49081 ttgttaaaac ctataataat caatgttcgg caaaaattat tcaaacagct atccgcaata
    49141 agacgcaaat aatctgtatt tctgaaccag ataaaaatta tggggaatgg gcttattatc
    49201 agttgaaaga acaaataata aataaaggga aaagatatgg aatagaagtt aaaagtgagt
    49261 agaataagaa atcgttgatt tggcaacgaa atagtgaggg gacactcact cactgaaggg
    49321 ttagctctgt caagttgtgt aaccagacgg aaggacgaat attgttgagg ttttagaacc
    49381 atgtaaaaca acacagttct caaatataga ttgatagatt gatcttccct aatctgtttt
    49441 agaaccatgt aaaacaacac agttctcaaa tgttacggat aaaccgtttg cgggaaatca
    49501 ggttttaaaa ccatgtaaaa caacacagtt ctcaaataag gctttattga attgatcagt
    49561 agtttctgtt ttagagttat ataaaacgac atggctctta aataggagct aacgaagaaa
    49621 aagaaggtac tccgttttgg agttaagtag aaataaaaaa atagaatcgt tgatttggca
    49681 acgagttgaa aaatacgcaa aactatggtc accaagggat aaccctgaaa ctgatgatcg
    49741 ctatatctta ctagtagtta aacagtctaa atagtactca tttattgtat tgattctaag
    49801 ggaacctgca ataaatgagt actattttac ttttaattcc gctatctatg tcattaggca
    49861 actaaattgt tattaatata tttacgttta ctcgtatatt tttagtaacg catctgtcaa
    49921 aactttagaa aaatttattt tatctttttc ccctctagct actaaccatt ctgggactgt
    49981 gacgtttttg cgtacggtct ttttttggat aaatggagaa ggatcacttt gaataagaga
    50041 aacaattttg ttattgccca aattatttag ttgatgaata tctgttggag aaggaactga
    50101 aatatcttca tcaatatagt aagctagaat cccatcaaga aattctttag cattggacat
    50161 agcttcttca aaattatctc cttgtgttgc tccagcaaaa tcaggaaaaa ctactgagta
    50221 gccattttct tcagcagaaa aaattacagg gtaacttttt ttcatttcca ccactccttt
    50281 cgagttaatc caatgaacta aaggactggg ctatttcagc cctaagtcct ttagtaactt
    50341 ctgctctgtc ccttttttta aatcctgatt actatgaaca ggaataggaa cgggtgggaa
    50401 tccttctttg gctacaatat gatggctgcc agttatcctc accactttcc aaccattttt
    50461 cttggctagt ttgagcattt ttttaccggt aagtggcatt cgcgactacc tccttacaaa
    50521 tattattata cgcattatgc gtataaaggt caagagtttt tacattaaaa gtagaatgta
    50581 agtgtcacaa tagggtatac cctattgtat catatggtaa agtacagtat aataaggttt
    50641 ggaggtgtaa cactagggtg taacacaatg tgaaatttgg aggtcatttt ttgaacaaga
    50701 aacaggatca agaatattat atagaaaaat tggagaaaga gaacttagaa ctcaaagagc
    50761 gaataagata ctatgaaacc aaattctgta aaagaggtga ttgtatgaaa ccaaatcctg
    50821 ttgaaacagg taaaagaatt aaatcaattc gttctaattt tggaatgact atggagcaat
    50881 tcgctatttt aactgattca tctaatacga gtgctgttaa taactgggaa cgtggttata
    50941 acttaccaaa taaaacaaaa ctaaaaaaaa tagctatact aggaaataca actactgact
    51001 ggattaaatg gggaacatta gaagagtata taacttcata ccttatcgat atagggtatg
    51061 agctatatat taaggacttt ccagagatac ctcacaaagt ctttaaagac atccaagaaa
    51121 gatatagtga tacgttttcg ctaaataaag actatgaatt attggattct ataataaaaa
    51181 atatatttac aaaatattac tcaaacggtt gaattgatag atggactcaa agaagaaaat
    51241 tcaaagttaa aagagaagat aaaatactat gaatgtttat ttgatggtat agagagtctg
    51301 ctgaactcac gaaaaaatcc ctagttttta tgaatgaccg ttttgtaaat ctgattgaaa
    51361 tagatttata tgatttcggt atatgatata cattaggagg tgtcgaatgg aaataaatct
    51421 ttttgaggct ggtaatcgaa taaagcaaat acgagaaaaa catggatata ctatggcaga
    51481 cttaggtaaa ctggttgatg ccaatagtcc tagcacaatt aacaattggg aaaagggaaa
    51541 taacttacct aatagaaagc gtcttgaaaa aattgcacta ttaggtggta cttctgttga
    51601 atggataaag tatggagatt tttccgatta tgtttatcgc ttgacacaaa atatagagga
    51661 aaaaattctt aaagagtacg gagagactac actgaacaac taccgtgaag ctcttctaga
    51721 tgaacttacg attgaaaaaa tcacatacga agaagataca aagatattag cagctgccga
    51781 aaaaatcaat acacaaaata tttttcagga ggtgaagtac atgtattctc ctgatgaaga
    51841 ttatcgaaat atagaagtca aaccagattt aggactgact gatgacaaca aaaatattaa
    51901 aatgattgga caacgtataa ggaaaataag aaaaaaagag aatattacat tagaagaatt
    51961 cggcaaacta ttctctccta cagctgataa agctgttata agcaactggg aaaatggtaa
    52021 aaaccttcct aactcagaaa gaattaaaaa gattgcagaa attggcggag tttctgaact
    52081 ttatcttatg acgggtgtcg attcaagcgt agcagaatca atgtattttc tatctgaagt
    52141 agcattggat gctttggaac gtctgtcaat tgaggaagtc aatagaataa ttgaaagctt
    52201 cgccggttat cttaatgtga tttcaaaaat tgatgatcca gataagcgag aaatatctct
    52261 caatgctatt tgtcaactat cacttctcga taaaatgggt tgattcacaa aaaatattga
    52321 aagattactt tactcacgag gaggcttagc tatgaaacct aatctatcgt taactagtga
    52381 acgtattaga aaaattatcg aatccaaagg tctgacatta gaacgattcg gtgatttaat
    52441 tggcgacaca ccaataagtt ctgttaataa ttggcttaga ggcgtgtctt tacccaataa
    52501 aaataggctt ttaacaattg caaatattgg aaacactagt gtagactgga ttaaatgggg
    52561 aactttggaa gaatacatag cttcatacct tatagattca ggttatgagc t
//
LOCUS       DAPBCS010000015        52002 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPBCS010000015 DAPBCS010000000
VERSION     DAPBCS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 52002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.015"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            23..694
                     /locus_tag="RD521_001068"
     CDS             23..694
                     /locus_tag="RD521_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7770817.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="RD521_001069"
     CDS             753..1193
                     /locus_tag="RD521_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770818.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="RD521_001070"
     CDS             1205..1873
                     /locus_tag="RD521_001070"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDT7770819.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="RD521_001071"
     CDS             1937..2560
                     /locus_tag="RD521_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDT7770820.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="RD521_001072"
     CDS             2616..3002
                     /locus_tag="RD521_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDT7770821.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="RD521_001073"
     CDS             3215..4606
                     /locus_tag="RD521_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDT7770822.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5217..5585
                     /locus_tag="RD521_001074"
     CDS             5217..5585
                     /locus_tag="RD521_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDT7770823.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5765..6706
                     /locus_tag="RD521_001075"
     CDS             5765..6706
                     /locus_tag="RD521_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDT7770824.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6775..7506
                     /locus_tag="RD521_001076"
     CDS             6775..7506
                     /locus_tag="RD521_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDT7770825.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7650..9830
                     /locus_tag="RD521_001077"
     CDS             7650..9830
                     /locus_tag="RD521_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDT7770826.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9850..10332
                     /locus_tag="RD521_001078"
     CDS             9850..10332
                     /locus_tag="RD521_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDT7770827.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10348..11109
                     /locus_tag="RD521_001079"
     CDS             10348..11109
                     /locus_tag="RD521_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDT7770828.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11240..12574
                     /locus_tag="RD521_001080"
     CDS             11240..12574
                     /locus_tag="RD521_001080"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDT7770829.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12881..13366
                     /locus_tag="RD521_001081"
     CDS             12881..13366
                     /locus_tag="RD521_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDT7770830.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13359..14042
                     /locus_tag="RD521_001082"
     CDS             13359..14042
                     /locus_tag="RD521_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDT7770831.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14168..16813
                     /gene="polA"
                     /locus_tag="RD521_001083"
     CDS             14168..16813
                     /gene="polA"
                     /locus_tag="RD521_001083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDT7770832.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16858..17694
                     /gene="mutM"
                     /locus_tag="RD521_001084"
     CDS             16858..17694
                     /gene="mutM"
                     /locus_tag="RD521_001084"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDT7770833.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17658..18287
                     /locus_tag="RD521_001085"
     CDS             17658..18287
                     /locus_tag="RD521_001085"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDT7770834.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            18339..18671
                     /locus_tag="RD521_001086"
     CDS             18339..18671
                     /locus_tag="RD521_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDT7770835.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18983..19474
                     /gene="nrdR"
                     /locus_tag="RD521_001087"
     CDS             18983..19474
                     /gene="nrdR"
                     /locus_tag="RD521_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDT7770836.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19497..20870
                     /locus_tag="RD521_001088"
     CDS             19497..20870
                     /locus_tag="RD521_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDT7770837.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20870..21796
                     /gene="dnaI"
                     /locus_tag="RD521_001089"
     CDS             20870..21796
                     /gene="dnaI"
                     /locus_tag="RD521_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDT7770838.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21925..22365
                     /locus_tag="RD521_001090"
     CDS             21925..22365
                     /locus_tag="RD521_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDT7770839.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22509..23210
                     /locus_tag="RD521_001091"
     CDS             22509..23210
                     /locus_tag="RD521_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDT7770840.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23207..24328
                     /locus_tag="RD521_001092"
     CDS             23207..24328
                     /locus_tag="RD521_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDT7770841.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24343..25575
                     /gene="pepT"
                     /locus_tag="RD521_001093"
     CDS             24343..25575
                     /gene="pepT"
                     /locus_tag="RD521_001093"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDT7770842.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25707..26615
                     /locus_tag="RD521_001094"
     CDS             25707..26615
                     /locus_tag="RD521_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDT7770843.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26650..26922
                     /locus_tag="RD521_001095"
     CDS             26650..26922
                     /locus_tag="RD521_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDT7770844.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26933..27979
                     /locus_tag="RD521_001096"
     CDS             26933..27979
                     /locus_tag="RD521_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDT7770845.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28230..30839
                     /gene="clpB"
                     /locus_tag="RD521_001097"
     CDS             28230..30839
                     /gene="clpB"
                     /locus_tag="RD521_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDT7770846.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30990..31676)
                     /locus_tag="RD521_001098"
     CDS             complement(30990..31676)
                     /locus_tag="RD521_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770847.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31654..32148)
                     /locus_tag="RD521_001099"
     CDS             complement(31654..32148)
                     /locus_tag="RD521_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDT7770848.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32168..33388)
                     /locus_tag="RD521_001100"
     CDS             complement(32168..33388)
                     /locus_tag="RD521_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7770849.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33535..35370
                     /gene="lepA"
                     /locus_tag="RD521_001101"
     CDS             33535..35370
                     /gene="lepA"
                     /locus_tag="RD521_001101"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDT7770850.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            35566..36477
                     /locus_tag="RD521_001102"
     CDS             35566..36477
                     /locus_tag="RD521_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770851.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            37473..37724
                     /locus_tag="RD521_001103"
     CDS             37473..37724
                     /locus_tag="RD521_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770852.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            37807..38079
                     /locus_tag="RD521_001104"
     CDS             37807..38079
                     /locus_tag="RD521_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770853.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            38169..38408
                     /locus_tag="RD521_001105"
     CDS             38169..38408
                     /locus_tag="RD521_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770854.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            38555..42838
                     /locus_tag="RD521_001106"
     CDS             38555..42838
                     /locus_tag="RD521_001106"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDT7770855.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            42937..43302
                     /locus_tag="RD521_001107"
     CDS             42937..43302
                     /locus_tag="RD521_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770856.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            43304..43756
                     /locus_tag="RD521_001108"
     CDS             43304..43756
                     /locus_tag="RD521_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770857.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            44370..45680
                     /locus_tag="RD521_001109"
     CDS             44370..45680
                     /locus_tag="RD521_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDT7770858.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            45703..46014
                     /locus_tag="RD521_001110"
     CDS             45703..46014
                     /locus_tag="RD521_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770859.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            46375..47631
                     /locus_tag="RD521_001111"
     CDS             46375..47631
                     /locus_tag="RD521_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7770860.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            47628..48173
                     /locus_tag="RD521_001112"
     CDS             47628..48173
                     /locus_tag="RD521_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770861.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            48174..48674
                     /locus_tag="RD521_001113"
     CDS             48174..48674
                     /locus_tag="RD521_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770862.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            48755..49311
                     /locus_tag="RD521_001114"
                     /pseudo
     CDS             48755..49311
                     /locus_tag="RD521_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            49331..49675
                     /locus_tag="RD521_001115"
     CDS             49331..49675
                     /locus_tag="RD521_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770863.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            49694..50605
                     /locus_tag="RD521_001116"
     CDS             49694..50605
                     /locus_tag="RD521_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7770864.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            50684..51676
                     /locus_tag="RD521_001117"
     CDS             50684..51676
                     /locus_tag="RD521_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7770865.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            51673..51891
                     /locus_tag="RD521_001118"
     CDS             51673..51891
                     /locus_tag="RD521_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770866.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
BASE COUNT        17705 a         8645 c        11147 g        14505 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aataagaggt
     4741 tgtgaaaaag ctgccctgca tcaagtaata agaacagcca aacaaaaata gttcctcata
     4801 ttttacgttt ggttgagctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
     4861 aggtcgtgag cctgccgttt cactctgagt cacaaagagc aatttcaaaa aatagcttct
     4921 catgtttgtt gaatattgtg tcttgtgacc gaggagttgt caggagaaca ccgtttacca
     4981 gaggttgtga cttgttttta gtcacgacct ctttttttat ttattccata taaatacaca
     5041 ttatttattc cgaaaagtga aagcgcaaat aacaaaaaca accaacttat ttattattaa
     5101 agaaaagcgt atttaagtag gacgattctg ttgagagctt tcgtagattt tcccattttc
     5161 ctatggtaaa ctgacaaagt tgagaatgtt agaacagatc gtttaggagg aaacagatgt
     5221 ttacgaatga aataaaaatc atgctttatg tggacgatgt agaaaaaaat gcaacgtttt
     5281 ggagagcagt tggctttgcg gaaaaagatc gtcaagaaat ggatgggacg cttataattg
     5341 aagtaggagt tgaggacagt caaacctcat ttctgttata tgatcgagca tttgttgagt
     5401 cccattcacc tggcacagct actagtgccc catctttgat gttttacagt aaagatatct
     5461 ttggactata taaaaaaatg cagacgcaat cagtcacatt aggtgatatg gtacagcttg
     5521 gagaagaata tgtctttaac tttgctgatc ccgatggcaa ctactttgcg gttacaggta
     5581 aataatagaa ataaagactt tgtttttctg tgcgaaacag gaaagcgaag tctttttaat
     5641 tcaaataata atattatgta aacaaaaaac tttccatgcg taattataca caagcaaata
     5701 aatcgtcaaa tgcgaacgcc ttctttatac tgtttaggaa cgaaaagaag gaaggaagaa
     5761 atacatggat tatcaagtac tgctttatta caattataca aaaatcgaag aaccagagga
     5821 atttgccaaa aatcatttag ccttttgcaa atctttggat cttaaaggtc gtattctagt
     5881 agctgaagaa ggaataaatg gtactgtgtc tggaaccgtt gaaaacacga ctgcctatat
     5941 gaaagcaatg aaagctgatc ctcgatttgc tgatacgatg ttcaagatcg atccaagcga
     6001 aggacatgcc tttaagaaga tgtttgtaag ataccgtcca gagcttgtaa gtttaaagct
     6061 agaagaggac attgatccaa accaagtgac aggtgcctat ctttcaccgc aagaatttaa
     6121 agaagctatt ttagatgaac agacagtggt gattgatgca cggaatgatt atgaatatga
     6181 cttgggacat tttcgagggg ctgtacgccc agatatacgc agttttcgag agcttccaga
     6241 ctggatccgc gagcacaaag aagaatttat ggaaaaacgg gtagtgacct actgtactgg
     6301 tggcattcgt tgcgagaagt tttctggttg gcttgtccgt gaaggattca aagatgtcgg
     6361 acagctgcat ggcggtattg ctacttatgg taaggatcct gaagtccaag gagaattatg
     6421 ggacggcaag atgtatgtgt ttgatgaacg gatcgctgtc gatatcaacc aagtagataa
     6481 acaaatcgtc ggtcgcgact ggtttgacgg aacaccatgt gaacgatata tcaattgtag
     6541 caatcctgag tgcaatcgtc agattcttgc ttccattgaa aatgaagaaa aacatttagg
     6601 agcatgtagc atagagtgtg cgaaacatcc gcgcaatcgc tatgttcaag accatggaat
     6661 ctctccagaa gaagcagaac tgcggtttac agctttaatt aaatagaaaa caatagtttt
     6721 gattgtcgac catttccaat atgcgctata ctagcgttga ggatggtgat ccacatgaaa
     6781 tcattgattt atgctattgg ggatgtccat ggcaaacatg agctttttga aaaacttatt
     6841 cactattatg atccaaagat ccatcagctt gttttattag gggatctcaa tgatcgaggt
     6901 cctcgtacaa aagaatgttt tttattagga aagcaattag tcgaagaaac aggagccatt
     6961 tacctccggg gaaaccatga agaatatttt ttgcaatttc tgcaatcacc ggaggactgg
     7021 tatgcgcctt atatgagaaa tggtggaaaa gaaacgtttg aaagcctgat tcacccaggg
     7081 gcatcggagg aatactcccc aactgaaatc tcgatgatga tccaatcgag atattcagaa
     7141 ctgattaaat ttttagtcca gcgccccttg tattatgaat gggaaaatta tctttttgtc
     7201 catgccggtg tggatttgac aaagaaagac tggcgtaaga caagtctaag agactttatt
     7261 tggataaggg aaccatttca tgaagggaaa aacaatacag gtaagacaat tgtatttgga
     7321 catacgatta caccaatgct ccatggagat atgcagacaa cagacctctg gattcaggat
     7381 cataaaatcg gaatggacgg tggggctgta ttcggagggt cattgcatgg ggttgttttt
     7441 gacaaggaag gtattcttca agatattgaa tatcagaaca acgaaggacc ttggcatgga
     7501 atctgactaa tggaaagaat gaaggagctg tgactttgtt gcggctcttt ttatttttat
     7561 agagaatatt gtttctttca gtagtttaac ccaggataaa aagtatgata gaataaagaa
     7621 gcaattttta gcagaaaaga ggcattttaa tgacggaaaa tttgaaccag acgatcattc
     7681 agcttgtaca aaaagaactt tcagactatc gtcctaagca gttgactact gtacttaatt
     7741 tgttaaatga aggaaacact gttcctttta tcgcgcgtta ccgaaaagaa atgactggaa
     7801 gtcttgatga agtccagatt cgtgaaatcg aagagcggta tgcctatctt gaaaacttag
     7861 aaaaacgtaa aactgaagtg attcggctaa tcgatgaaca agggaaactg actcccgagt
     7921 tagaagcaga aatcacccaa gccgtgaaaa tgcaacaagt ggaagatttg tatcgtccat
     7981 acaaacaaaa acgacggaca aaagcaacga ttgctaaaga aaaaggactt gaaccactcg
     8041 cgaagtggct gatgcagctt acagatggag aggtgcaatc tgaagcagaa aaatatattg
     8101 acaaagaaaa agaagtctct tctgccgaag aagcgttgca cggtgctcat gagattattg
     8161 ctgaacaagt cagcgataat gcaaaatttc ggacttggat tcggtcttat acgtataaca
     8221 aaggtatgta tgtcagcaat gtcaaggatg agcaagcaga tgaaaaaggc gtatatgaaa
     8281 tgtattatga ctttgcagaa cccgtccata aaatggtttc tcatcgaata ttagctacta
     8341 atcgtggaga aaaagaagat attctgaaag tattctttca agtagatgaa gcagcaattt
     8401 tagcttactt ggatcgccaa atagtcaaaa atcctgcctc tccttcttct tcattcgtca
     8461 gagaagccta tcaggatagt tataaacgct ttattcagcc agcaatcgag cgagaactgc
     8521 gcaatgaatt gactgaaaag gcagatgagc aagcaatcgc tattttcggc gaaaacctcc
     8581 gtaatctttt gttgcaaccg ccactaaaag gtaaagttgt cctgggattt gatccggctt
     8641 atcgtactgg atgtaaacta gcagtcgtag atgcgacagg gaaagtttta gcaatcgaag
     8701 ttatttatcc tcataaacct gcagcgcaag caaaaagaga agctgctgga ccagctttta
     8761 tccaattaat taataaatat caagtagata tggtagctat cggaaatggt acagccagtc
     8821 gggaatctga actttttgtg gctgagcagt tgaaatccgc cgaccataaa acctattacg
     8881 ctatcgtcaa cgaagcaggc gcttctgttt actccgctag cgaaattgca cgaaaagaat
     8941 ttccgcatct acaagttgaa gaaagaagtg ctgtttctat tgcccgtcgc ctgcaagatc
     9001 ctttagctga attagtcaaa atcgatccta aagctgtcgg agtaggccag taccaacatg
     9061 acgtttctca aaaacgtttg gctgaacaat tggattttgt tgtggaaaca gcggttaacc
     9121 aagtaggtgt ggatgtgaat acagcaagcc cgcagctctt gcagcatata tcaggattga
     9181 ataaaacaac tgcccaaaac attgtcattt atcgtgaaga aaacggagaa ttcaccgcac
     9241 gaactcaatt gaaaaaagtg ccacgcttag gaccaaaagc atatgaacaa gcaatcggat
     9301 tcttgcgagt ccctggtggg aaaaacattt tggacaacac aggtatccat cccgaaagtt
     9361 attctattgc aaaagaaata ttaacttcag tccaactttc agaaaaagag cttggtacag
     9421 aagaagcagt agaaaaattg acacgattat ccgtagagaa actggctgaa agcctatcag
     9481 tcggagaaga aacgttagcc gatattttag ctgggctgac acaacctgga cgagatatgc
     9541 gtgatgagat gccagcgccc ttattacgta cagatgtatt gagtatggaa gacttgaaac
     9601 cagggatgga attgactgga acggtccgta atgtcattga cttcggcgca tttgttgata
     9661 tcggtgtgaa gcaagacgga cttgtccata tatcgaaact cagcaaaaaa ttcgtcaagc
     9721 atcctacaga tgtcgtgtca gtaggagata tcgtaacagt ttggatagaa caagtcgata
     9781 cgaaaaaagg cagaatcagt ttgacgatgc tttcccctta tgaagagtaa caatgaggag
     9841 taacaaaaat tgacacaaga agaactgcag cagttagtag aagagatatc attaaaaagc
     9901 ttccagcttc ctttcaagca ccaagcaatg ttcaatccac gcttacgtac aactggcggg
     9961 cgctatcatt taaacgatca tcatttagat tttaatgcga agctatttga agagtcagac
    10021 gaagagacga ttgcaggaat catcaaacat gaactttgcc attatcatct tcatctagct
    10081 ggaagagggt atcagcataa agatatggat ttcaagtatc ttttgaaaaa aacaggaggg
    10141 ttgcgctatg caccagcctt gacgaagaaa cagataaaga tggagtatta tcgatgccaa
    10201 gattgttctg ccacgatctg ccgcaagaga agaatcgatg tacgcaaata tcgttgtgga
    10261 agatgtcatg gagaaatcaa atggattgag acaaaaagag aagactttaa tgaagcacaa
    10321 aaaatcagat gaaaggatag aaggcctttg gaaaaaagtg agcagaggaa tctaaaagac
    10381 tggaatcagc tattaaattt tttagccgca cctgaagagg gaaattttga ggaactgacg
    10441 gcacctacac ttattttagc tggaaactgt cttcctatct tagctgataa agcagcacaa
    10501 atgtatttaa atggccaagt agatcaattg tttttggtcg gaggggtggg gcatgcgact
    10561 agaatccttt atgagaattt tgaaaagcaa ggatttcatt ttgaagatgg catgagtgaa
    10621 agtgaaatct gtcgtcagta tctaaaagaa gtctatgatt tgcccgataa ggcttttctg
    10681 atagaatcga agtctacaaa ttctggagag aacgccactt tttcactgga gattcttcat
    10741 tccttaggag ctgtaccaga aaaaatctta ttgatgaatg atccgacact gcaacgcagg
    10801 actcgagcaa cttttgagaa agtatggcaa aatgaacaaa ctattttcgc gaatgctgtg
    10861 ccatttgtgc cggaaatcct tcacttttct gaagagatca tctttacagc aaaagaactg
    10921 aatcatcaat ggcctaagga atatttccat gcgctagtat taggagaaat ggaacgtctc
    10981 catgacgatg agaacggcta tggtccgaaa ggaaaagatt ttattcctca cattgatata
    11041 tcagaggaag tttggtcttc ttacaccaga attaaagagt caataaaaac ggatttttct
    11101 cgtacttgac cattttagca agcgaatcct cctttccaaa taaatttggt tgaaattttc
    11161 aatcgtttat aagataatga ggagcgtgtt gaatgtaata gctaaaagaa tggacgctag
    11221 ataagagaag aggattcaaa tggaaaatca aaataaattg tatcattttg ttggtatcaa
    11281 aggttcagga atgagctcct tggctcttgt tttacatgaa caaggtctaa atgtccaagg
    11341 atcagacatt gaaaaatatt tctttacaca aagagattta gaaaaagcaa atatcacgat
    11401 tctgccattt aacgcagata atgtaaaacc agggatgacg atcattgcag gaaatgcatt
    11461 tcctgattca catgaagaaa tccagcgagc aaaagaatta ggtttggaag tgattcgcta
    11521 tcacgacttt attggtcatt ttatccaaaa ttatacgagt atcgctgtaa caggctctca
    11581 cggaaaaaca agtacgactg gactgctctc tcatgtattg agtggtgtcc gtccaacaag
    11641 ttatctaatt ggagatggaa caggacatgg tgatccgcaa gcggaattct tttcatttga
    11701 ggcctgtgaa tatcgccgtc atttccttgc ctattcacca gactatgcaa tcatgaccaa
    11761 tatcgatttt gatcatccgg attattatac aagtatcgac gatgtattta cagctttcca
    11821 aacaatggct ggacaagtga aaaaagctat ttttgcgtat ggtgacgatg cttatttacg
    11881 caaattaaaa gcaaatgtgc cgatttatta ttatggtgtc acagaaaacg atgatatcca
    11941 agcacgaaac atcgaacgca caacttctgg ttctgctttt gatgtctatc atggggatga
    12001 atttgttggg cacttcactg taccagcatt cggtaaacac aatatcttaa atgctttagg
    12061 tgttattgca gtagcttatt tcgaaaaact ggatctgaaa gaagttgcag aagaaatgct
    12121 gactttccca ggagtaaaac gtcgattcag tgaaaagatc gtagcagaca tgaccgtagt
    12181 cgatgattat gcgcatcacc ctgcagagat caaagcaacg attgatggcg cacgccaaaa
    12241 atatccagat aaagaaataa ttgcagtttt ccaaccccat acgtttacgc gtacgattgc
    12301 attgatggat gaatttgcag aagcacttga tttagctgat aaagtttact tatgcgatat
    12361 ttttggttcg gctcgggaag aacaaggaaa cgttaagatt gaagaccttg gagcgaaaat
    12421 caaaaaaggt ggagaagtca tcaaagaaaa taatgtatct ccattgttag attatcatga
    12481 tgcagtagta atcttcatgg gtgctggaga tgttcaaaaa tttgaacaag cgtatgaaaa
    12541 acttttaagc agcacgacga aaaatgtact gtaatacaat tactgcagtg tccttataaa
    12601 aatagaaaga gggggagtgg caaaaatctt gccaagcccc cttttttgca acttatgata
    12661 aacggtgttc aaaagcttgc atctgcggta ataagcccaa tcaaatcaaa aacatgagaa
    12721 gctgtttttt cttggctgtt cttattattt gatgcaggac agctttttca cgacctctgt
    12781 aaactatagg aatctgttaa atcaagggag cagaacttgc cttttacgct acttttggta
    12841 caataatgta tgattaaaaa taaactggag ggtttctatc ttgaacatag gtaagccgag
    12901 aaaactagga aaaacaataa atgaaatcga ggaaggcgac tcgttatcct taacagaatc
    12961 tattgaagat aaccagcttt tgctgtattt gggattgacg aatgatgcaa atcctttgta
    13021 tatccaacat gactttgcac aaaaaacaga atatcaacag ccaatcgttc catcagtgat
    13081 gctgatgggg atcatcacaa gtgcaatttc aaagcatctt cctggaccag gatcacatgt
    13141 tgtcaatttt tctgtcaatt ttattgaacc ggttttccat tatgaaacac tgacttttca
    13201 gtttgaggtc ataaaagtag ataagatgaa ggatgtggtg acgatttctg tagaagcgga
    13261 gaatacgaac gaagatcgtg tgttggatgc tgtcgttatg gtccaaccgc cacaagttcc
    13321 tgaattggaa atagcaatcg aaagtgaagg tgaattgaat gaataatcca agtacatcaa
    13381 tgtcaggtat caatcgtttt tatgcaaaag tttatggatt tttaggtatg ggacttgccc
    13441 ttagtgcagt cacttcttac ttggctcttt gggtatttcc tttgcaatta gcaactttta
    13501 tcaataattt tccactaggc tttgtgggtc tgtggatcgt tgaactgatt ttagtaatgg
    13561 ttctaggcgt aaaagcacaa aagaatccga gcttagcaat cggtggattt attgcctact
    13621 ctcttttgaa tggcataaca ttagcggtta ctcttgcata ctacgatatt ggaacagtaa
    13681 cgcaagcttt cttgacagct actttgatgt tcatggggat gtctgttgtc ggagtaacaa
    13741 caaaaagaga tcttagcggg gttggaagag caggacaggc agctttaatc ggagtgatca
    13801 ttgcactagt cttgaacatc ttcattttga gaagtagcgt agttgagctg ttcatctccc
    13861 tactgttagt cgtcatcttt gcagggatta cagcttatga taaccaaagc atccgtaata
    13921 tctatgtcca gacaaacgga acagcacata gtggaattgc tgtattcatg gcattgaagc
    13981 tgtatttgga ctttatcaac ttgttcttgg cgtttctgag aatctttgga cggaacaact
    14041 aaaaaagaaa aaaattcatc agttggggct ataccgaatt atttggtatg gtcccgcttt
    14101 ttcttttcgc attccgcagg attctttgtt agaatgaata agaactttac agtaaggagt
    14161 taagaagatg acaaaaaata aattattact cgttgatggg aacagtgtgg cttttcgcgc
    14221 attctttgcg cttcacaatt ctcttgaacg tttcaaaaat agaaacggtc ttcatacaaa
    14281 tgcaatctat gcattcaata cgatgtttga aaatgttatg caaaaagagc aaccgactca
    14341 tgttctggtt gcgttcgatg caggaaaaac gacttttcgg acagaaatgt atgcggagta
    14401 caaaggtgga agatccaaaa cacctggcga attcaaagaa caaatgccat atatacgtga
    14461 tttgttgaca ggactagggg tacagtatta cgaattgccc aattatgaag cagatgatat
    14521 tatcggtact ttggcagaaa aagtagacaa agatcaattt gatgtcgtcg tgctttcagg
    14581 tgatcgggat ttgactcaat tagctagtaa agaagtaaaa gtggatatca ctgtaaaagg
    14641 tgtcagcgat atcgaaagct atacaccaga acatgtagcg gaaaaatatg atgggttgac
    14701 gccaaaacag attattgaca tgaagggact agccggagat gcctctgaca atatccccgg
    14761 tgtgactaaa atcggtgaaa aaacagctat caagctattg aaacaatatg gctcagtcga
    14821 aggaatttat gaacatatcg atgaaatgaa gcaaagcaaa atgaaagaaa atctgataaa
    14881 tgacaaagag caggctttct tatcgaaaaa attggcgacc atcaatactt cagcgcctgt
    14941 ggatgtctct atcgactcat tgaagtatga agggaaaaat ctcgataaac tcgttccgtt
    15001 ttataaagaa atggatttca atcagttcct ttctaaattg aatatcgtag aagaagaagt
    15061 aaaaatggat gatattttgt ttgaagtcgt tcatgaattc aaagaggaaa tgtttactac
    15121 ggatatggcg ttgtacgtgg aaatgatggg cgacaattat catacagagg aaatcgtcgg
    15181 agtagcgtgg gggacagaaa agaaaattta tgtcacgaac gatctatctg tattcgaaaa
    15241 ccgagcgttc cattcttgga tcactgatcc gactcggctg aaaaaagtat atgatgcgaa
    15301 acgaacttat gttgctttaa accgctatac tggaaaaacg aaaggaatcg attttgatgt
    15361 cttgctaggt gcataccttc tcgatacgaa tgaaaaaagc acagatatcg aaggaatagc
    15421 tgctcattac ggatacaacg atatccaatc agatgaagct gtatacggca aaggggcgaa
    15481 aaaaggttta ccagaagaag aggaactctt ttttgctcat ttagcgcgaa aagtagctgc
    15541 aatcaatgcg ttgacaccaa aattgagcca agaactggca gaaaaaaacc aagaagatct
    15601 tttccggaaa atggaactgc cattgtctat cattttggca gagatggaaa taagcgggat
    15661 caaagtagat gcgactcgct tacaagaaat gaagggcgag ttctctgctc gcttaagaga
    15721 gatcgaacaa aaaatctacg aagaagccgg cgaagaattc aatttgaatt caccaaagca
    15781 attaggagtg atcttatttg aaaaaatggg attacccgtt atcaaaaaaa caaaaactgg
    15841 atattccaca gccgtagatg tactggaaca gcttcgtgaa caagcaccaa tcgtagagga
    15901 tatcctcacc tatcgtcaaa ttgcaaaaat tcaatctacc tatgtcgagg gactgctgaa
    15961 agtgatccaa tcagatggaa aagtgcatac ccgttatgtc cagacattga cccaaacagg
    16021 acgattaagc tctgttgatc ctaatttgca gaacatcccg attcgattag atgaagggag
    16081 aaagatacgt caggcatttg ttcctagaga aaaagactgg ctgatttatt cttctgacta
    16141 ttcacaaatc gagttacgcg tacttgcgca tatctcaaat gatgaacatt tgaaagctgc
    16201 cttcctagaa ggtcaagata tccatgccag cacagcgatg cgtgtatttg gaatcgaaaa
    16261 ggcagaggat gtcacaccaa atatgcgtag gcaggcaaaa gcagtgaatt ttggtatcgt
    16321 gtatggcatc tctgattacg gactttcaca aaatctagga atcagccgaa aagcagcaca
    16381 gcaatacatc gatacgtact ttgaaaaata cccaggtgtc aaagaatata tggaaagaat
    16441 cgtacgcgaa gcaaaagacc aaggctacgt cgaaacgctg tatcaccgcc gtcgatatct
    16501 gccagacatc aattcaagaa attacaatat ccgttctttt gcggaacgaa cagctatcaa
    16561 tacaccaatc caaggaagtg ctgctgatat tttgaaaatc gcgatgatcg aattggataa
    16621 acgattaaaa gaaacaggtc ttcaagctac gatgctgtta caagttcatg atgaacttgt
    16681 cttcgaggta ccggaaaaag aacttgaatc attagataaa ttggtgaagg aagttatgga
    16741 acaagctgtt tctcttcatg taccactgat cactgacagc agttggggaa aaacgtggta
    16801 cgaagcaaaa taaacattgt ttaagacaga gcaatataat gaaggtgaaa agaaaaaatg
    16861 ccagaattac cagaagtaga aaccgttcgt aaaggattgg aacggttagt agtaggaaaa
    16921 acgattcaaa aagttcaggt cttgtggccc aagatcatcg agcaaccaga gacacccatc
    16981 tttgaggctt ctcttgttgg cgaaaccatc caatccattg gacgtagagg gaagttttta
    17041 atttttcatc tcgatcattg tgaactgatc tcacacttgc gaatggaagg aaaatatcaa
    17101 tttacgaaag aaaatacacc aatcgataaa catacgcatg tacttttctt ttttgaggat
    17161 ggaagccaac ttcgctacaa cgatgtacga aaatttggtc gtatgacaat cgtcgaaaaa
    17221 ggagcaagtg caacttaccg aggaatcatg aaattgggac ctgagccttt gccagatagc
    17281 ttcttattag cagatttcgc aaatggactg aaaaagtccc acaaagcaat caagccattg
    17341 ctgctagacc aacgtctagt gactggtcta ggtaatattt atgtagatga agcattatgg
    17401 gaagcgaaga tccatccaga acaaccggcg aacacactgc gccccaaaga ggtagaacaa
    17461 ttacgtctat cgatcattga tgttttggat cgtgcaatag aagccggagg taccacgatt
    17521 cgcagttatt tgaatgcatt aggagaatca ggcggatttc aagtagcact gcatgtctat
    17581 cagcaaactg gcaaaccatg tatacgttgt ggaacgccga tcgtgaaaac aaaagtggca
    17641 caaaggggga cgcactattg tccgaattgt caaaggctaa aaatcgcaaa gtaggatttg
    17701 ttttaggttt gacgggtgga atagctaccg gtaaaagtac ggcagccaaa gtctttcagt
    17761 ctcatggatt tcctttgatc gatggcgatg tgattgccag agaaactgtt gagcctggaa
    17821 cgcttgctct gaggaaaatt gtatcagtgt ttggccaaga ggtattgcag cctgatgggc
    17881 aactagatcg agggaaactt gggatgattg tttttccatc aaaggaaaaa cggcaaaaac
    17941 ttgatcagct attagatccg tttatccgaa aagcgataaa agatcaaatc gcctctctaa
    18001 gttccaatca tcctttagta attgtagata tcccattgct ctacgaaggt cattatgacc
    18061 actatatgga tgcagtagct gtcgtttata ctacgccaga aactcagctt cagcgtttga
    18121 tgaaacgtaa tcaactgacg ctagaacaag cgcagcagag aattagtagt caacttccaa
    18181 tcgaagaaaa aaagcagcgt gcggatattt tgttcgacaa caatggaaca aaagaaaaac
    18241 ttgtgggaca aatcgaaaat tggctttttg ctaatcgctt tctttgagta tctgtgtcaa
    18301 ttttgctatc ctgagtatat agaaagtgag ggatagcgat gggatcatta aaaaatcaaa
    18361 aggataaagt cgtaggtaac gcaaaagaaa aaatcggcga gatgctcaat aaagaaaatc
    18421 taatcgatga aggtcgtacg caatcagaaa cagcagaaga aaaagaaaag cagtaccaag
    18481 caagaatcga tgaaatagaa gaccagcagg cagaagaaga agcgaagcgg caagatttga
    18541 ctcaagaacg aaaaaatccg acacctgatc gtagtaaaac accgctccca gaaagtgacc
    18601 acattcccga tgagcaaaaa atcgaggaac agcgaatgaa acactataca ggaatcgaag
    18661 agaagttata aaatagtaaa aaatcgagat gtgacttgaa gcagatgaga aggtgttcat
    18721 ctgcttcaag ttttttgtgc tagatgacta aatcagcttt tgtgtaagtt atttctcagt
    18781 aatcaaggtg atcagtattg gaaaattgaa atttagtaag gacaggggac acaattacgc
    18841 atgaaagtag aaaaaattgt tcttatatcc gtgttttttg ggtaggacag tcgcgtttgt
    18901 gatttgtttc gtgttataat acaactagtt gtaaagaaag acacatcctt tatataaaat
    18961 gcgatgcaag aggtgagaca aaatgagatg tcccagatgt caacataata attcacgagt
    19021 aatcgacagc cgtcaagcag atgatggacg tgcgatccgt cgccgtcgcg aatgtgagaa
    19081 ttgttcgttc cgattcacta catttgaacg aattgaagct gctccgttat tagtaatcaa
    19141 gaaaaacggg gaacgcgaag agttcaatcg cgataaaatt ttaagaggac tcatccgttc
    19201 agcagaaaaa agacctgtag ctatggaaca aatggaacaa atcgtcaacc atgtcgaaaa
    19261 ccgcgtacgc agtctcggag aaaacgaagt atcgacgaat ctaattggtg aatatgtgat
    19321 ggaagattta gttgatttag acgaaatcgc ttatattcgt tttgcaagtg tttatcgcca
    19381 gttcaaagat atgtctgttt tcttaaaaga gttgcaagat atcgtggata aagcaaaaga
    19441 aaataataaa gagaataata aagaaaacaa ttaagaaaca aaaaggaggc atttccttgg
    19501 aaaatgcttg gaaagagctt caaccaaaaa atatttacca aggaagaaaa gcggctccgc
    19561 tgagagaaga cagcaagaac gccttgctgt atctttatca gccactaatc ggcggagaag
    19621 ccttgagtat ctatttcacc ctgttatcag aaatctctca agaatctggc aaaggtcctg
    19681 aagggatgca tgcagatctg cttagtagtc taggctgcgg tatcccgcaa ttttatgatg
    19741 caagaaaaaa gctagaagga atcggcttac tggatgttta ttttaaagaa gatagtgatc
    19801 tgggacctcg ttttatttat gagctgttag aaccactcat tcctaaagca tttttcaaag
    19861 actctgttct ggcttttctt cttctggaaa aagttggtga acgacgattc cagaagctta
    19921 cccaattctt tgaacctaaa aggcatacag cagagggata tcaaaaaatc acaaaaaaat
    19981 ttttagatgt ttatcgcttc aatgaagcag tgtacgctgc taatcaagaa aacatcgaat
    20041 cgctgaataa atcttttgaa gaatctgaat cagtagcatt acttgaagca gattcgacat
    20101 tcgattggca gtttttgaat gattggctgg caaaaaaaca tattgcaagt cttgatgatc
    20161 caatcatcaa acaagtcgac atgtatcatc agctttacgg tttcgatgaa ttgacacttg
    20221 gcgaactgat cagtcaatct tatgacttta gtgaacaaaa agtatctgaa aaagaactgc
    20281 aaaaagccat tttggcaatg cagagacgtc cggtgaagca aacaaaagaa acagttgcgg
    20341 tttctgaaaa cagtaatgaa ccctcgtcaa acgaacattt gacaaacgca gcgcttcagc
    20401 tgataaaaga agcacagagc attccaccga tgaaatatct ggaagcaatc aagtcagaaa
    20461 aaggcggata tgtttctaaa caagaaaact ggttactgca agatcttgtt tctcaatcag
    20521 gtctttccag tagcgtgatc aatgttttgc tgaattatgt actagtgatc aagaataatg
    20581 ctagcttaaa tgccagttat gtaaatacag tggcaaatga atgggcacaa aagaaaatcg
    20641 ccacagcaga agaagcgatc cagcacattc ggcaaataag taatcaagca aaacaatcaa
    20701 aacaaaagaa gcaaacaaat tatcaaactg gcaagcgaaa tgttcgacgg gaaaagcttc
    20761 ctgattgggt caaccagcca aaagatgaaa aacagatcag cagcgagaaa aaagcagaga
    20821 ttgaccgccg ttttaaagaa tacctggcaa aaaaggaggg tgatatctag tggaagacgt
    20881 aggcaaagaa ttgaagagga tcatcagcaa acgcaattat caagaaaagt atgctgaaat
    20941 gatcgctgaa gtcttaaatg atgcggatgt tcaagctttt ctaagcgcac accaagaaga
    21001 attgacacaa gcggatattg aaaaaagtta cgctaaattg tatgaattcg tgcaagaaaa
    21061 gcgtaaatat caaaaaaacg atccaacgat gatcgctccc ggctatgagc cgaagctgac
    21121 gttgaatttt cattatgtgg atgtcactta tgtaccaacg gaagcattgt tagcacggca
    21181 aagacaagaa gaaatcagaa gccgagtaaa agcaatggat atgccaaaag atattcaatc
    21241 ggcttctttt gaaaactttg aacgtacaga tggacgagga tatgcgagct tagaagccct
    21301 agattttgta gagaaatata ctgctgatcc aaaagggtat cataaaggac tgtatctagt
    21361 aggaagtttt ggtattggaa aaacatattt attaggagca acagcgagag aattagcgat
    21421 tgctggattc accacgacgc tagtccattt tccgacattt gccgttgaga tgaaacaggc
    21481 aatcggaaaa gatcaagtgg cagaaaagtt agatgcagtg aaacgttcac ctattttgat
    21541 gatcgatgac attggtgcag atgcgatgag tagttggata cgagacgaag tattcggtgt
    21601 tatccttcaa tatcggatgc aggaacagtt accaaccttc ttctcatcga attttacaat
    21661 ggatgaattg gaacagcatt tgtccgtcac tcaacgtgga gatgaagagc cgctaaaagc
    21721 gaaacggata atggaacgca tacgttattt gacaaaagaa atcgaaatga ctggtcgcaa
    21781 ccgacgcaat ggttgaacag ttgacaagtc caactgacat ttgacctagt agtaaaagca
    21841 ggtcagcgaa tcattatatg gacagaaatg accggagaca atcaccgcaa caagtaatgc
    21901 agaaacttaa acggaggatt ttttatgagg gctatttttt ctagtatatc tgtagttggt
    21961 ctattttttc ttttagcagg ctgcggtagc gcagatagca acacaggttc tgttgattcg
    22021 attgatgcgg gtcaaacagt aaaaacaagc agtacgaaaa cggagagaca aagcatattt
    22081 gtcggaacga ttgaaagtat cactcaagta gaagcagaag aagaaacgct tcagttgaaa
    22141 ctcgtcgatg tcatggccaa agaagatcca gcgaatatgg gaacgtcatt cagcaatgac
    22201 ggagtgatct taaatgctac tgctgaaaaa ataagcgtgg acataaatga attgaaaatc
    22261 ggtgacaaag tccaatttac attagttgag atgccgatca tgacgatgtc tatcccaccg
    22321 caagttcctg gagattcgat tattgatgtt tcattgaaca aataaccaat aacatttttt
    22381 caaaaataag aaaatgttgt tttatagaac gaaaaggtat attttttaag gagatatggg
    22441 aattttttta ttcctagtct ccttatttta tttggaaaat gctataatta tctagaagga
    22501 gtgaagctat gaaccattta gatttatctc agcgcttagc cgcagtgggt gactttgtcc
    22561 ctgaatccgc tcgtttagca gacattggat cagatcacgc ttacttaccc gttgcattga
    22621 tattaagagg aaaaatcgaa tatgcagttg ctggtgaagt cgttcaaggt ccttatgaat
    22681 cagcacgtaa gcaagtcaaa aaagatggat tagaagaccg tatcaccgta cgccttgcaa
    22741 atggtctaga tgctatccag ccacaagatg gtattacagc tgtcacgatt gcgggtatgg
    22801 gaggttcact gatccgagat atcctagaag ctggcaagtt gaatcaacgc ttaagcggac
    22861 aagaacgttt gatccttcag ccaaacatcg gagagcgtac attacgatct tggctgcaag
    22921 aaaatcagta taaaatccaa tcagaaaaaa tacttgaaga aaataaaaaa atatatgaaa
    22981 tcattgtagc cgaaaaagaa caaaaaccag ttacttacaa tgaaatggag ctgatgttcg
    23041 ggccatttct gcttgaagag aaaagtgaga tcttcaaatc aaaatggaca agagagcttc
    23101 aacaaagaaa agctatccgt gaacagctca aacaagcagc taatcaagag gcacgtcaaa
    23161 aacaaatcga gcaggaaatc caaatgattg aggaggtttt aaagccatga caatcagcgg
    23221 acgcactttc atccaaaagt ttgaaagcta ttgtcctcag tggctagctg aagaaggaga
    23281 cccagtcgga ctgcatatcg gaacattgga taagccaatc caacgagtaa tgatgacatt
    23341 agacgttcgt ccagaagtgg tcgaagaagc aatcaagaaa aaaatcgatt tgctgattgc
    23401 taaacatcct ccaatctttc gtcctgtaaa acggctggtt actgatcagc cacaagaaaa
    23461 aatgtatgca gatttgttga aacatgatat tgccgtctat gcagcacaca cgaacatgga
    23521 tattatttgg gatggactga atgactggtt ttgtgaactt ctcggtattg aagtggagag
    23581 ttatttagta aagactcatg agattcatta taaaaaactg gccgtttatg tacctgtgga
    23641 tcatgcacag aaaatgcgtg aagtattagc tgcagctggt gctggtacac aaggtgatta
    23701 taccggtaca agttttacct cgattggaca cggtcgattt actccggaag ctggtgcaca
    23761 accagcgatt gggaaagtag ggaagacaga acaagttcaa gaggcaaaag ttgaggttat
    23821 tttgccggaa actatcgaaa aacaagtgat tcaagccatg cggtcagctc atccttatga
    23881 ggaacctgct tatgatcttt tcgctataga tgaaccagtc gaaatgtttg ggttaggacg
    23941 agtaggtgaa ttgccgcaag aaatttctat agaggcattt gtagaacaag taaaagaagc
    24001 gttccaacta gatggactga gaatagtcca gccaaaaaat gcaaaatcct ctgtgaagcg
    24061 cattgcgatt tgcggtggaa gcggtgaaaa attttatccg caagcaatcg cccaaagagc
    24121 agatgtctat atcacgggag atatctatta tcatacggct catgatatgc aaagcgctgg
    24181 gttgattgca attgatcccg gtcattatat tgaatcgtta tgcaagcaac gatttatcga
    24241 gaaatttgaa tcttggaaac aagaagaaaa ttgggatatt aactttttcg tttctgaaac
    24301 aaatacaaac ccatttcaat ttaattaaaa ggagagaata ctatgtacga aaatcttttg
    24361 ccacgttttt tacgatatgt caaaacagaa acacgctccg atgcaacaag caaaacgact
    24421 ccatcgactc agacacaagt tgcttttgcg caagaattga aaaaagaatt agaagagtta
    24481 ggcttagctg atgttttcta taatgaagat aacggattcg ttatgggaac attaccaagc
    24541 aatgttgatc atgaagtgcg ttccattgga tttatttccc acatggatac agcagatttt
    24601 aatgcagtag gagtcaatcc acaaatcatt gaaaactatg atggcgagtc tacgatcgta
    24661 ctagatgcgg aagaacgcta tacattgaat acaaaagatt ttccaaattt gaaaaattat
    24721 gaaggccaca cattgatcac aacagatggc agtacattac tgggagcaga tgacaaatca
    24781 gggattgcgg aaatcatgac tgcaatggag atcttgatca ataatccttc tatccctcac
    24841 ggtaccatca aagtggcttt tggtcctgat gaagaaatcg gtgtcggagc ggataaattc
    24901 gatgttgagc aattcgatgt agattttgct tatacagtcg atggtggtcc tttaggtgag
    24961 ttgcagtatg aaacgtttag tgctgctcaa gcaaatatta cgatccaagg gaaaaatgta
    25021 catccaggga cggcaaaaga tacaatgatc aatgctctac agttagcaat cgattttcat
    25081 aatcagcttc cagccgatga agttccagaa aaaacggaag gatacgaagg tttcttccat
    25141 ttgatggcat tggatgggaa tcctgaagaa gcttctatga gttatatcat tcgcgatcat
    25201 gatcgtgaaa aattcgaagc acgcaaagca caaatcacac atatccaaga agagttgaat
    25261 cagcgatttg atcaagaacg aatcaaagta gatatgtatg atcaatatta caacatgaaa
    25321 gaaatcattg aaaaagatat gagcatcgtg gaattagcga aacaagcaat gatcgagctt
    25381 ggtatctctc cagtgatcga accagttcgt ggaggaacag atggttcaaa aatctcttac
    25441 atgggtattc cgacgccaaa tctatttgcc ggaggagaaa atatgcacgg acgattcgaa
    25501 tttgtttctt tacaagtaat ggaaaaagca acagacgtga ttgtaaaaat tgcagaattg
    25561 aacacaaaga gttaaaatcg aaaaagaaca gcttatgaca aggcgttcgt cccaatgatg
    25621 gaatgtcatc tgtctaaggc taaagggcag gacaatggaa ttggttgtcc cgccctcttt
    25681 tttgaagagt aaataggagg taaagaatgg aaaagctgct agttttttat aacaaaagtt
    25741 cagggaaaga tgagggtgag aaattagcac gctggtttca agattatgta aaagaaaagc
    25801 agccagaatt atatgtagaa ctagctgaaa ctggcccatc gattgatgat gcggaattag
    25861 tcaagaaagc agaagccata gaagctgata ctttagtaat tataggtgga gacggcacga
    25921 ttcatcatat cgtccaagcg ttccaaaaaa cattatctca ataccatgtc ggattacttc
    25981 caggtggaac tgtcaacaac cttgcacgtg ttcttgagat tcccctcaaa aaagaagaag
    26041 ctgctgacgt gatcctggaa ggcaatgtac ggaaaataga ctatggtcaa gtcaatgatg
    26101 aagtcattat ctctacactg acaatcggaa tcttagcaga tacagcagtt tggatcagtc
    26161 aagaagaaaa acagcgttac ggctcgtgga tctttatcaa gaagtttttt aagttattgc
    26221 tgaagaagaa aaaatatcat ttggatttag aaacagacga ggagcattgg aaaggaaaat
    26281 cccaactgtt gacagtcacg atgtctaact ccgtgggcgg ttttacaaat tttgacgact
    26341 cagcgaccct agatgatgga aaatttcatg taacgatcgt accttcctta gatattttcc
    26401 gatttatttt ttatttgcct agaatcatga gaggcaaatt ctatacgatc cccggcatta
    26461 cttatttcac agccaaacgc atgaaaatcc aagcgcagga aaaatctgtg ggcactcgaa
    26521 cggatggaga cacgacagat gatcttccag ttgaattatc tgttgtatca aaaggattaa
    26581 cagtttattc accaaagaaa gaagaaaaag cttagaaata gtagttaaca gaaagttgga
    26641 agaagagtaa tggatgtatt gttacacaat cctttgtggt tttacctttt cattgtgaat
    26701 ttttacttat tttgtctgat ggggtatgat aaacatcagg ctagaactag cggctggcgt
    26761 gttcctgaga ataatttgat atttgtcagc ctgttaggcg gtggtttggg aggattgatc
    26821 tctcgaaaag ttttccatca taaaactcgg aaaaagaagt ttactatagg attcgctgca
    26881 ggtgtacttg ttgatttgat tctcatttat tttttccatt aaggagcagg agatgtcgtc
    26941 taaaggaacg aaaactaaaa tcattctgaa tctgcttttg ctgattgtga tttttgccat
    27001 tatttattat ttaatcaatc aatcatttgc agatatcttt gccgaattgc tgtctaccag
    27061 tgttgttgtt ttggcagcgg ctgttatatt agggacagtt taccaaatcg ctgaagggcg
    27121 ctcaatcaaa gaaattgctg aacctttcgc gccgaatttt acaacgaaag atgggttctt
    27181 gacctctagc tatattgcgt tttaccgaat cgtttcgttt ggtacaggaa cgctgatctc
    27241 ggagatttat ttttataaca aaaaaggaat gaagctatcc cagggagcag gggtgacagc
    27301 actccatatg attatgtaca aactggcagt gatcacttat gcattagctg gcttggtcat
    27361 ccaattctct cttttttatt ctaatggtcc atcaatgatc tggtttatca tcgctggtat
    27421 ccttttgacg atcgtgattg ttgccttttt attggcgatg tccgtcagct tgaatgttca
    27481 aatatttttt gtcagcttat cgaataagtt atttaaaagt gagcgtttgc ggaaatgggt
    27541 ggatacatgc aatcagcaga tctactcttt aagggaaacg gtccaaacca ttatccaaga
    27601 caggactgcg ctattgcgga tttatctatg gaatatgtta aaacttgtat tttggtatgt
    27661 acttccttat ttgattctga ttgaaaacca tcctaaaata gattttctac tgaccatttc
    27721 gtttatcagc tttgcagtag tgctttcagg ggttattcct acgcctgctg gtattggttc
    27781 ctttgaattt gtctatttgt tattattcag accagttgtt ggaacagttg acgcagtttc
    27841 ttcgctttta ctgtatcgtt tcagttcctt tatcttgcca tttctctatg gatttgtgta
    27901 tgtgattgct gaacggcgaa gagtgatcaa agaagaaata atagaagtaa aaaaagagaa
    27961 taaagaaaat caagggtagg tcttttataa gatctaccct ttttatcaaa tcaaagaaaa
    28021 acgttatgga taagataatt ttacgtttgt ttggctcttt ttttatatac atacaatcaa
    28081 ttttgttttt ttaagattta tataagtcga gaaaaaaagt caaaaaacta tgtgtatatt
    28141 ttgaccaaaa ttgaccaaag cgatataata gtttttgtaa agatcaaaga aataagtgat
    28201 cttaccttta attaggaggg tatacctgta tgaatattga gaaaatgact acgacgcttc
    28261 aagaagcgat tgctgaagct caacagattg cggtcactcg aaaacatcaa gatatcgaca
    28321 ttgcccatgt atggaaaatc tttttacaat ctaatcattt tggtcgaaat ttctatacgg
    28381 atgccggatt ggatgtcgaa tcttttgaac atgaaattga tcgtctactg gatgagtatc
    28441 cagttgtttc tggtggaaac gtccaatatg gacaaaacct cagccagaat ttctttcgat
    28501 tattgaatga agcagaccaa atacgtgaaa gcttcggcga cgagtttctt tcaacggaag
    28561 tcgttatttt agcgttgatg aaattaaaaa attatccgtt aactgtttat ttgaataaaa
    28621 acggattatc tgaaaaagaa ttgcgtaaaa acatcgaaga gatgagagga ggagaaagag
    28681 tgacctcaca aaaccaagaa gaacaataca aagcattaga aaaatacggc gttgatttag
    28741 tacagcaagt caaaagcgga aaaatggatc caatcatcgg tcgtgatgaa gaaatccgtg
    28801 atgtcatccg tattctttca cgaaaaacaa aaaataatcc tgttctgata ggagaacccg
    28861 gtgtagggaa aacggcaatc gttgaagggc ttgctcaacg tatcgtacgc aaagatgtgc
    28921 ctgaaaattt aaaagataaa acgatttttt cattagatat gggtgcattg atcgctggag
    28981 caaaattccg tggtgaattt gaagaaagac taaaagcagt cttgaaagaa gtcaaaaaaa
    29041 gtgatggacg tatcctcttg ttcatcgatg aaatccacaa tatcgtaggg gcaggaaaaa
    29101 cagaaggaag tatggatgcc ggaaatctgt tgaaaccaat gcttgctcgc ggtgaattac
    29161 atttgatcgg tgccacaact ttagatgagt accgtcaata tatggaaaaa gacaaagcgt
    29221 tggaacgtcg ctttcaaaag gtattagtca aagaaccaac agtggaagat acgatcagta
    29281 ttctgcgtgg attaaaagaa cgttttgaga tccaccatgg tgttaacatc catgacaacg
    29341 cattagtagc agcagctact ttatctgacc gttatattac tgatcggttc ttaccggaca
    29401 aagcaatcga tttgatcgat gaagccagtg caacgattcg ggtagaaatg aactctatgc
    29461 caacggaatt agatcaagtt actagacgtt tgatgcagtt ggaaatcgaa gaagctgcac
    29521 tgaaaaaaga atcagatgat gcaagtaaaa aacgcttgaa aaatttgcaa gaagaattag
    29581 ctgaacttcg tgaagaagca aatgcgatga agatgcagtg ggaaacagaa aaagaagaag
    29641 tcaattctgt ttcagcaaaa cgtgctgaaa tcgataaagc aaaacacgaa ttagaagatg
    29701 cagaaaataa ctatgattta gaacgcgctg ctgtattgcg tcatggtacg atacctcaat
    29761 tagaaaaaga attgaaggaa ttagaagcta aagcaaaaga cagcgagatt aagatggttc
    29821 aagagtccgt taccgaaaat gagattgctc aagtcgttgg cagattaacg ggtatccccg
    29881 taacgaaact tgtagaaggt gaacgagaaa aactaattaa attgaatgaa acacttcata
    29941 aacgagtgat tggtcaagat gaagcagttg atgcggtcag tgacgcggta attcgttcac
    30001 gggctggatt gcaagaccca aatcgtcctc taggttcttt ccttttccta ggaccaactg
    30061 gtgtcggaaa aaccgaatta gcgaaagcgt tagctgagaa cttgtttgac tcagaagatc
    30121 atatggtacg gatcgatatg agtgaatata tggaaaaaca tgctgtgtct cgtctagtcg
    30181 gagctcctcc aggctatgta ggttacgaag aaggtggtca attaacggaa gctgtcagac
    30241 gaaatcctta tacaatcgtc ttgttggatg aaatcgaaaa agcgcatccc gatgtcttca
    30301 atatcttgtt acaagtccta gatgacggac gcttgactga ttcaaaaggg cgagtagtag
    30361 atttcaaaaa tacggttctg atcatgacca gcaatatcgg ttcacagtta ctgctggaag
    30421 gcgtgactgc tgatggaacg attccagaag cagtggctga acaagtcaac acgctattac
    30481 gtggaaactt caaaccagaa ttcttgaacc gaatcgacga cacgatttta tttacaccat
    30541 tgagcctaga caacgtgaaa ggaatcgttg acaaaatggt ggcgcaattg gcacaacgtc
    30601 tggaacatca agaaatcctg ctgacaatca gtgatgaagc caaaacgtgg attgctgaaa
    30661 atgcttatga accagcatat ggtgcaagac cattgaaacg atttatcact aaggaagtag
    30721 aaacaccact agctaaagaa atcgttgcag gacatgttat gcctaaatca aaagtaacga
    30781 ttactttact tgatggacaa cttcatttca aaacagaaga gctagaagaa atcgtctaat
    30841 cttccattga gactataaaa gaaaaaaggg gtctgacaca actagtgtca gacctccttt
    30901 tttcaaataa acagtgttcc agaagtagtt tcttcgaaaa ataagccgaa atttcttgaa
    30961 gctgcccgct tctgtcacaa cctcttttat taggtatttt tcactttttc taatttataa
    31021 ctttcaaagt aacgctcatc ttttttactt tgagaccatg caaacaatcc cattaaggca
    31081 gcacctatcg tatttgtcag caagtcgccc atcgtatcca tcaatgctgc tcgtccgaca
    31141 aacagtgtgc catcagaagc agcaaaacgt tgaagattca tgcctaagaa ctggtcgcat
    31201 aagaattccc agaactccca aaacacgccg caaagtccag caaacgcaaa gccgaataag
    31261 aggaacaacc aaggactggt ttttgatatc tcagcatctt tcatcaagta accaatcaat
    31321 ccatagccaa gagctgtcag gaccattgga ctcaccgcat gtaagatttt gtcccaaaaa
    31381 gagatgatac tgatcaaatg catccctgtg cctaaaaaga cagagatgaa caagaaaaac
    31441 cagtaaaaca aaacgattgc tgctggtatt ttaagtttga aaactttcaa aatgacttgt
    31501 ggcatgaaaa tcaatgcaat ccctaataaa gattccacga taaatgtgat tccttgagaa
    31561 gtcgaatatt tttgtgtgat aaattcataa aaattatagc agacaacgaa tacaccaaaa
    31621 gcaagtaaag ctatttgcac ctttttgatt tctttaatca ctgaaagtcc ctccatctaa
    31681 aaatcttttt acttctgaaa gcaactcaat ttctgaagaa accacttttc cgttcaattt
    31741 gatcaaacct attgtatata agttcaggta gtgaaattga ttttcagctg tttcttgtag
    31801 tgcagcgatt tttttcgggt gggttccgcc ttgctgtcga gtatcagtat ataagccgat
    31861 cactgggatg cctttcgcat aagccacacc gatttccgat gctaccccgg catcgatcgt
    31921 cagcccatct agtaaagcaa tcattaaatc actagccaac actttttctg tgtcagcaag
    31981 agcaatcatt cgactgtctg cataagcagt tttgtcattg attgcttcat tttcttgagg
    32041 aagatagatc gaaagctcag aagagagctc tctgatttct ttaactaact gttggttata
    32101 taacaaatca cttttagcaa acattggtgc ggcaaaataa attttcatct aaacatcatc
    32161 ctttttatca aaaataatcg ctaaagtaag cttccatgtt tggaatgata cgctctagtg
    32221 acttcagtgt ttcttcatct gtttctaacc gttcgattct tgccaaataa gaagcacggc
    32281 gataattcat catcaagcaa gtcaatgttt ggatatccag tcgaacggat tttcctatag
    32341 gttcatttaa gacacggact tgtccctgtt catcccatgt taaagcgaag acaccattat
    32401 tccaaggagc gacaggatct tcaatgatga aatgaaaagc atcagctgtt ccaacaaacg
    32461 gaaaccgttg aaggaattct tttacatcaa cgatccgagc cataaaatat ggttcgatct
    32521 gctctttgat ctgactatct tcaatcaaaa atgctaaagg ctcattttta taaatatctc
    32581 catgtaccca gtagatcatg gaaaaatgtg cggaaataaa gttccataag ccatttcgag
    32641 cttcttgatc aagatagaac atttctttta cgtgaaatat ttcttcagct acccagtaga
    32701 acagaacacc tcttggttca tggttgctat cgtagtagac agcggctgtg cgctcatctt
    32761 cattttcgaa acgccaatac tcttcccaat gaaaatcact gcggaataaa gcaccatggt
    32821 tttgttgggc aaatttgtca taaacggaaa atacgtctgg atgatcaact ggtaatcgct
    32881 caaccatacc acttactggg atttgtttag gtagctgtgt atctctgatt ttaaaggaaa
    32941 gtttatcaga catgatttcc catcctttac gccgatagta ggggatactg taaggataca
    33001 aataagagat ccattgctta ttcttgcgca tattatccaa tgcaacgata attagatctt
    33061 gcatcaatcc atggttagca tattctggat aagttccaac tccagtcaca ccacccattt
    33121 tataacgtgc accgtgaata ttgacttcac aaggatagat agcgatttga gagatcagtt
    33181 tattttcgtg gaaccatccg aacactttcg atacttccaa aatcggtttt ttggaacgga
    33241 taaaagctcg tttattctca aacccgcttt cctcgatgtc tgcttcagtc acttggaaca
    33301 cataggaaag caattcattg aattgatcga tatgttcttg agtgaccggt tttaattcaa
    33361 gattttctct aaaatctttt tgatccattt tttcatctcc atttattagc ttgataacag
    33421 tatagcaaaa ttctacaaaa aaaacttcta tagctgacta attaaaaact ctttaaatta
    33481 ctgcttttcg ttggtataat ggaaagtgct gattatatga aaagaggaga cgctatggat
    33541 ataaagaaaa tgaaagaacg ccaagagcag attcgtaatt tttcgatcat cgctcatatc
    33601 gaccatggga aatctacatt ggccgatcgt attttagaaa tgacgcacac tgtgacgtct
    33661 cgcgaaatgc aagatcagct gttagactcg atggatttgg aacgtgaacg tggaatcacg
    33721 attaaattaa atgctgtaga actcaattac acagcgaaaa atggagaaac atatatcttt
    33781 cacttgattg acaccccagg gcacgtcgac ttcacctatg aagtctctcg aagccttgct
    33841 gcctgtgagg gtgccgtttt agtggtcgat gcagcacaag gaatcgaagc acaaacattg
    33901 gccaatgttt atttagcgtt agacaatgat ttggagatcc tgccagtcat caataaaatc
    33961 gacctacccg cagcagatcc cgaacgtgtc cgtcaagaaa tcgaagacgt aatcggaatc
    34021 gatgctagtg aagctgtatt agccagtgcc aaatcaggga ttgggattga agatattctc
    34081 gagcagatcg tggaatatgt tcctgcacca agtggagact tagatgcacc gctaaaagct
    34141 ttgatctttg actctattta tgatagttac cgcggtgttg ttctaaatgt ccggatcacg
    34201 gacggtatgg tcaaacccgg agacaaaatc aaactgatga gtaatggcaa aacatttgat
    34261 gttacagagg tcggggtctt ctcgccaaaa gcagttgcta gagacttttt gatggtcggt
    34321 gatgtcggtt atatcacagc aagtatcaaa actgtccaag atacacgagt aggggataca
    34381 gtaacgttag cagacaatcc tgccgctgaa gctttaccag gttaccgaaa aatgaatccg
    34441 atggtttatt gtggtcttta tccaattgat acttctcgtt acaacgattt gcgggaagct
    34501 ttagaaaaac tgcaattgaa tgatgctgct ttacaattcg aaccagaaac ctctcaagca
    34561 ttaggttttg ggttccgctg tggattcctc ggacttcttc atatggatgt cgtccaagaa
    34621 cgcttggaac gggaattcaa tctagaattg atcacgactg caccatccgt tatttaccat
    34681 gttaataaaa cggatggtac gactgctaca gtagataacc cggcagattt tcctgaacct
    34741 gtaacgattc aggatgtaga agaaccattc gtcaaagcac agatcatggt cccaaatgat
    34801 tttgtcggtg cagtaatgga actttctcaa cgaaaacggg gagaattcat tacgatggat
    34861 tatttagatg attaccgagt aaatgtcgtc tataatattc cgctttctga aattgtcttt
    34921 gactttttcg acaaattgaa atcaagcacg aaaggctatg cctcattgga ttatgaaatg
    34981 tctggttatc aaaaaagcaa gttagtgaag atggacatct tgctgaatgg agaaaaagtc
    35041 gatgcattaa gctttatcgt tcaccgagac tttgcctatg aacgtggaaa agcaatcgtt
    35101 gaaaaactga aaaaactaat cccacgccaa caatttgagg tgccaatcca agcagctatt
    35161 ggacaaaaaa tcgttgcccg ttcagacatc aaagccttgc gtaaaaacgt cttggctaaa
    35221 tgttatggtg gagatgtttc tcgtaaacgt aaactcttag agaaacaaaa agaagggaag
    35281 aagcggatga aacaaatcgg ctccgtcgaa gttccgcaag aagccttcat ggccgttctg
    35341 aaaatggatg agcaagatag tgtgaaataa cttataatca atcgaaaaac ctattaaata
    35401 aacctttttg agtagatgca acactgagga ttatctgtat aaaagatttt aaactgtcat
    35461 ttggttatca cggcgatact aaaattaaca aaattggaat atttttcttg aagattttta
    35521 gctgtcagta tatgatgaaa gcatctcata ggaggtgctt ttttcatggt tgataattca
    35581 agtggaaggg ttcttaaatc tctaagaaaa gagaaaaaac tatctcaaaa aaagttagct
    35641 gatttagcag gtatttcaca aagtacttta gttaaatatg aaaaagggtc aagaaaaatc
    35701 cctaaagacg tagataatac tctaagcaag attctgaata tagaaactct aataaaagat
    35761 gaggaagata agattgagat tctcatcggt aaactaattg catacagaga tatgaataag
    35821 ttacttaaca aagagttagc tgataatata ggaattagtg aagtattact cagttatgtg
    35881 ttaaatagaa agagaaatcc gtcaaaagaa atgcaaaaaa aaatcgatat ttttctatta
    35941 agtaatgaaa aagaaatttt gaaggagatt aatcgtgata gtgagatatt tagtttatca
    36001 aaagacgata aaattgttat gggaaaaaga atacgagaag tccgaaagaa ccgggaagag
    36061 acgcttgaaa aatttggtaa aaactttacc atctatacag gcaaaaatgt tataagtaga
    36121 tgggaaaaag gtattaatat tccagatatt gaaaaactaa tgaatattgc ctatcttggt
    36181 aaggtcactg ttccttacct tatgtacgga gaagattata agaatatact tccaaaagat
    36241 gaaagagtta gcgattttaa aaagataaat tcttttagta tgggattaag gatgaggaaa
    36301 attaggaaag attattatct agaaagggag gagtttggaa agcttttttc tccttcaatt
    36361 tcaaaatggt ccatagatag gtatgagaat ggtagagata tacccaatac taatcgaatt
    36421 atccaatatg cttatatagg aaatctttct ttagaatttt tgatttacgg gatataaaaa
    36481 tagtttacgt cgatacttat taagttatcg acgtttttta ttatagaaaa aatttataaa
    36541 aggaggtttc ttgatatgat aagaaacaaa aagggtttgt tgaaagttgc tttactggca
    36601 gcttttttct aagtaacttt ttgacaagct caatggcaac acaagcggaa gaagtaatac
    36661 aagaactaac tgttatgaat aacagctaga taaaatgggc tatgtatgtt atccaacaat
    36721 ttagtaatca ctagaagcct agcgaaagtt cgcttgataa gttatagatg agtattgatt
    36781 tgtagtgttt ccaaatcaaa agggtacaga gccacatgaa atatgttgtc ctctgcagcg
    36841 tcaggatggg aatccatacc atggagattc ggagatctgt gagggaatac ttacgtggtt
    36901 aatatgtcag ttgtgttgga cgtagtaatg gtcatagtgg gtagattgac tgaattctta
    36961 tatgagaaaa tgaaaccaac aagaagatag ggttatttat acagaattgg ggatcttttc
    37021 tggaaaagta atcggataat gcgagattcg tacggtcttt tttaaatttc tctaaaaaaa
    37081 acggcatcga atgggttggg tgattgataa gcaatgaatc atagactgat atagaataaa
    37141 aaatgattct atttatcttt ttacatctat cgttttttca agtagccgca aattatatca
    37201 gcattcaaca atttttgtat acaagttcta gtaaaaggat ctgaagaaaa agttggttgt
    37261 tgtgaagata aaaaaagaat caggttttac tgagtttttc ttcaaaattt gtgttattag
    37321 gttaggacaa agataagaag aagtatgaag atttaaaaaa ggaatgagga aaagatagta
    37381 atttccgatt attggagttt ttttatttat aaagaagaag cgaataagtt gtgattccta
    37441 gatttcgcgc gtgattaatt aatgagcaaa aaatgcggaa agttttagtg tttaatacac
    37501 gaggacaaag tttcttaggt attgaaggca cacgtgtaac ggttgatggg tggtgtgatt
    37561 gtttcgaggt tggaaaacat gttgaactta tggcatttac tgagctaaaa gataagaaca
    37621 acgtggaatt tttcgagagt gatattggtt gggacgatca tgaagaagtt tatggaaaat
    37681 ttattttgcg gaagggaaat tcttatacga atggggaagc ataagtagcg atttgttaga
    37741 gatcattgat aggattgaaa ttgtcgggaa tatttatgag aatctagagt tatttgaggt
    37801 gatagaatga gtgatggttt tgaacaagta tcaataatta aaaatcaagt tcgggagatt
    37861 cttagaaaaa aaggctattt ggtagatagt tattttgaag gagattacga aacatgggta
    37921 ggtgtctatg ctcgtccaga gaataaacct acctatttag atcctacgac gtcagaagat
    37981 gcctatttac aaaataggta tcgagtagat ggttttaagc aagactttgc ggagtggttt
    38041 gaatgggaaa ttgaaaacgg agaagtaaaa gaagaataag agtacttgtt gtatcaaaat
    38101 aagccagtag aacaaccaaa ccagtagcta gtaatgacaa taatactgag gaatcggatc
    38161 attatggtat gattgatatt tctcttccag aagatcttaa gccagatcct gtaacaattg
    38221 gcaatagtgt catgattttt gattcattag atgaagctat gtcatatggt tggaaagaaa
    38281 ttaaggatga aggtagtaat tggtatggca tgagatttgg tgatggtgaa gttgccaaaa
    38341 aagctggtat tccaattggg aaatggacag ttgatttcca cgaagcaggt tctttttttg
    38401 ttctgtaatt atagtgggag ggtaaaatca ttgatgaaat gtgttcagat aagagcgcca
    38461 gataaaaata gaagagacag ttcacgtcgt tatcactttt ggtaacggcg ttttgtttta
    38521 ttatataaaa ttctcaggaa aaggaggaat tcttgtgtat gcaaaaacga aaatgaattg
    38581 gttgaaagca ctcgcattgg tggctttttc tcaattctgt ttcttggtgg gaactgctca
    38641 agcacatgca gaagaaacga cggctataca acctacgatg gaagccattg cccaagtcgc
    38701 ttctgaagtg acagaagtcc aaccagcaga agccggggtc ccagctggtg aagccactgt
    38761 tgaacaggcc cctgtgacag aagcgccaac ggtcaattcc aaaacgactc tcgatcagac
    38821 gattggggaa acacaacacc cagggaatag tcctggtact tcacaaccag ttgaagaaac
    38881 tgcctcaatt acaacccctg cttcagaaac gtccactcta gaaggagaaa aagctgccga
    38941 agcaacgcct tctgtgcagc cagagtcttc tgagaaacaa gcggaagaaa agaaacgaat
    39001 agaagaaaca gtggaacaac cagcggctac tgctgacaaa gaaggaaaaa atgaggaaaa
    39061 cgttgaaaaa acaatctctt caaccgcagc tagtcaaaat caaccaacga aaccaaacaa
    39121 agaatctacg gctagtattg aaaatcaaaa gaaaaaagag cagaacattc caaaaacagg
    39181 tacacctaca ctgttagtaa cagtaccaac caatcgttcg gttcgtgttc gccgtgcaac
    39241 caatgcagct gttttaggtg gtcggaaaat tgattggggc gaaaatattg gtgttacttc
    39301 tagtccgaac aatctaaact tcactttaaa ttatatggtt cgttttgaga ttaatggaca
    39361 agtggcgttt tgtattgagc ctggtgtctc agccgcagaa ggtcaagttt atacagctac
    39421 tgctttgaaa aactatttga aaaattcaaa tgtacggaaa aaaacaagct tgatctctta
    39481 tttaggatat gtcacaaata aagacaaatc cgatgaacaa tacattgcgg cacaatttat
    39541 gatttgggaa gctttgggca caaaaatccc aatcaaaaat atccgtgttg attatgatcg
    39601 tcgaaaagca gaaattcaac gtttgattaa taactttggt gaaaaagcaa cttttaataa
    39661 tcaaacacat acggtaaaaa ttggcgaaac gttgaaagta aagaataaaa atgatttttt
    39721 gaaatatgtg aaagacattc aaaaaccaaa aggtgtcaat gtatctattc acggcaatga
    39781 agtatggatt acagtcacaa aagaggcacc ggataaagca aaaattcaat tgaataaaat
    39841 taaagttcaa ggtgcgccat tagcctatcg tcgtccagga gctcaaacaa ttggtgtgtt
    39901 acatccaaca gaacctaatg cgtctgttat taatttacaa attctaaaac aaggaaacat
    39961 tcgtgtgttt aaagaagatg cggaaactgg tgtgaaagca caaggtgcag cttctttaga
    40021 aaatgcggtc tatggtttgt atcaagcaga tgggaaaaag attaaagaaa ttaccttgaa
    40081 aaaaattaac ggcaaagtac aggcagaaat taaagatctt gatcctggca aatatgtttt
    40141 aaaagaaatc aaagcgccaa aaggttatgt gctttctgat gaagcaattg aaattgttgt
    40201 tgaacctgga aaaactgtca atgcgaccgc aaaagatcaa gtgatcaaag gaaccattga
    40261 tttagtgaaa gttgctaata aagatttagt cgattcaacg aatccagata acaaaccaaa
    40321 attagctggc attgaaatca ctttgacgtc aaaaacaact ggcaaagtgg tgaaaaccgt
    40381 tgtgacagac aatgatggtt atgctacttt tggtaaaaat accgttgtct ttgatacgta
    40441 cattctttct gaaaccaaag gaaaagaagg gtataaacta tttgaaccac ttgaagtcac
    40501 aatttctgaa aatgggcaaa ctttccatta tgttttagaa gataaagtaa ttgaacaacg
    40561 tcttaaagtg gtgaaagtgg atcaagaaac aggaaaagtg attccacttg ctaatacaga
    40621 attcaagatt tttgatactt tagccaatcg ttatgtcaca atggagattc caaatgatac
    40681 agaaacaacg gatatcttta aaaccaatga aaaaggcttt ttcctaacta acggtacact
    40741 tacttatgga aaagatcgct atcgtttgga agaaatcaaa gcccccgtaa attacgtttt
    40801 gaatcaaaac ccattagtgt tctcgattac caatgataca gaaccgatca aagtgatcca
    40861 ttttgcgaac cgtcaagcac ggaaaagtgt aaaattattc aagtatgaag aatggaacaa
    40921 gaagaaaacg ccgctcgcag gtgtgaactt taccttgtat aacggtaaaa atcaagcaat
    40981 tggtgaatat accactgatc aaaacgggga aattttagtg aaaggtctag ttgccgatga
    41041 ctattacttc ttggaaaatg ccccattaga aagtttccaa ccaaatacag ataaacaagc
    41101 attcacggtt gcattcaaac aaaatgcgcc agatgatggg gaaacgttag tggtttctgt
    41161 acgtaattac ttgattccac caaaactaga aacacaagcg tcaaataaag ccgatggtga
    41221 aaaagtggtt gatccattag aaacgatcgt cattaaagac agtgtttctt ataccaactt
    41281 gtttgtcgga aaagaataca ccatttcagg agtattgatg aacaaggcaa cgaatgcgcc
    41341 aattttacaa gatggcaaag aaatcacaag taccttggcc tttattgcag acaaagtcaa
    41401 tggcacaaaa gaccttgaat ttgtggttaa tgctagtgtt ctgaaaggtc aaacgatcgt
    41461 tgtttttgaa aaacttttcc gtgatggcat tgaagttgca gcacatgcgg atatcaacga
    41521 tgaagcccaa agtgtgaaag tgaataatcc aaccgttcat accacggctg tctttgaaga
    41581 cggattgaca gtcgcagacc caacaggatt gtttacctta aacgataccg ttgaaattac
    41641 cgagattctt ccaggaaaag catacttggt caaagggatc ttaatggata aagcaaccgg
    41701 tgaacccttg cttgtgcatg gaaatacggt agaaacgagt gttggcttta ttgccaaagc
    41761 agaaaatgaa acggtgatcg taccatttac ctttgatttg acaggtataa atggtcgtga
    41821 aatcgtcgtg tttgaaagtc tttttagaaa tgatgaagaa ctagcaaacc ataaagacat
    41881 caacgacaaa gggcaaacgg tacgagtaac taacccaagt gtacgtacaa cggctacaca
    41941 taaacccact ggcttgaaag aggttaatcc attaacgaaa gtcaccatta cagacgctgt
    42001 gaaataccac gatttggtcg ttggaaaaac atacacagtc aacggtgttc taatggacaa
    42061 agtaacggaa aaaccagtgc tagtagatgg aaaagaagtc acaagtacct taacgtttgt
    42121 ggcagaaaca acggatggta cagtcaatct agactttatt ctagacgcac gtgcgttgcg
    42181 aggcaaagaa gtcgtggtct ttgaagatct gtttagagaa ggcgtattat tggcttctca
    42241 cgcagatatt accgacaatg accaaaccat caaaatcagt aatccaacca ttacgacgaa
    42301 agcgacgaat aaaaatggcg gaaaagagat cttggccttg ccaaatcaaa ctgtgcttga
    42361 atgggtcaaa gtagacggtc ttgcgattgg tcaaatctat cgcttaattg ttcaaggcta
    42421 cttaacacca gacggcacac catttgaagg cactcatgcg gaaaaagtct ttgcggccga
    42481 taaagaaatg atggaattct tatttgaatt cttagtggac ggtcgcaatc ttgcaggaaa
    42541 aggcttgagt tttgcggaat ggctccaagc gaaaacggaa gatgagaaag aaacgtttga
    42601 agaagtggcg aaacacaatg tagatctaac aaacaaagat caaacggttc aaattgtgga
    42661 agcgccaaaa ccaccgcaac cagtgaaacc agaacaacgg gttaaaccag ctactgttgt
    42721 accaatgttg ccaaaaacag gtgaacaaac aagtagtata cttatggttc ttggattagt
    42781 agtattagtc attgctttag gtataacact ttataaagta cgtaaagcca aagattagtc
    42841 atgaggaaca agcggttagg atttttaacc gcttgtttga ttgtaatgta gaaatattta
    42901 gaatttattt tggtttttaa gaggaggaaa aactttatgg cagaaaaagc attgcgtatg
    42961 aagaaagagt ttttaggtca agtattttta ttgggttcac gggaacctaa tccaccaaag
    43021 gaaattaatg gtgtgtctgg gcgatatgtt caattacaag cagagtgttc gcgagaagaa
    43081 cagcctgctt tttttgttta tctcacagct gaagaggata aaagattacg attaccagaa
    43141 aaagaggtgc gtatttcatt tgaagaagct tattttgttt ttcgtaaaga acgagtgaat
    43201 agtcgtgaaa caaatgatat gggacaaaca gagcgtcgaa caactggcta tcaagatgtt
    43261 ggtgtctttt taactggtgt aaaaattggg gaggttttgt aagatgggat taaaatttcc
    43321 aaataattat gacattacag aagcattaga tgaaacttgt ttaggagccg tacgctttgg
    43381 cgcattagaa gagtctgatc gtgtatttga taatgaaaca ggcgattata cgggagaaat
    43441 tcgatcacag cgagcacaat tagtttttgg aaatccagat gaaccaaaga ttgcacgacg
    43501 agttaaattt ccagcagatg tagatttgac tggttttaaa cgacgtgatc ctgtgaaatt
    43561 aattgatcca gaatatcatg tggtatatat tccagcacaa gatggcaatc gtgaatattc
    43621 agatttagtg atttatgcga aaggattaga gaaagtagaa gctccaacaa ataatcctaa
    43681 tatgaaacaa tcacaagaaa aaggtttaga tcaaaaacaa ccaagtgttg attctaagga
    43741 aaaagagaaa aaataaggtt tttttgaaag aataggaagg agtcttccta ttctttttat
    43801 ctaagataga aggaagggga gattttgaat tcagataaaa ccaatcaaat actttgagag
    43861 attgtgcttg ttatcaaaat atccaacaat tgattgagta ttttggagac aatccaacga
    43921 tttttgtaca aacaaatgca tatatttgtg cggttattgg gcagtgtatg tttttacgtg
    43981 atcttgttcg tttttcatta ccgtaccagt ggaaacaaga tcatcaggaa gtgatttgga
    44041 ttcgtatagt acgaatatgt aatatgtgtg cacaccctta taaaaatcga ttaaatcttt
    44101 ttcgattatg tttttttact aaatgtgata ttcctgtatt agttgctttt tgtcaaaagt
    44161 ttttagcaca ggaaaagtaa aaacagaaag gaggaatgtg acaattgaaa gctcattttt
    44221 tcaagtatcg tggtcggcga attcgctatt tcaatcgtta tttatggttt tgctattatg
    44281 gtttcttttt gtttccattt ttgattctgt taggttggtt tagttggaaa gtattttatc
    44341 cacgtttaag tacgttttcg aattggcaaa tgtatcttat tcctggaata agtattgtgt
    44401 tgtatttgtt gctggttagt gggttaattt tagggttgat gcattggtcg cgactaaaag
    44461 aaggatattt tgcctctgtt tattggcgtc agttacttgt tcgaatgtta attgacaatc
    44521 ggtttgttta tacaaagaaa caagttagtg agaaacaaac aagggaaaaa atgagattac
    44581 caaaagttta tttttatcaa aagaaacaag aattagtggt ttcttttcct ttagatggtg
    44641 ggcgttttca cgaacgattt ttaaaaatgg gtcatttgtt atctgaagta tttttgcgtg
    44701 atttaattcg tgaagaacga gaaaaagccc gaatcaatta tgtattcctt tcagattctg
    44761 gtcggattcc tattgatcaa tgtatagcag aaaatggacg tattcattta atgaaaactt
    44821 tggattgggt atatgatcaa tcaccaaata tgttgattgc tggtgcaatc ggtggtggga
    44881 aaacctattt attgtattct ttaattcaag cttgtttaag tgttgggaca gtagatattt
    44941 gcgatggaaa agcggcagat ttagcagcat taggtgacgt cgatgtattc aaaggacatg
    45001 tgttttacaa aataaatgaa gaaatgatta tttgtttacg aaatgcatta aaagaaatga
    45061 atcaacggta tgcctatatg aaaaccatga aagatcctaa atatgaacca gggaaaaatt
    45121 atgcttatta tgggttacca ccgcatttta tcttttttga tgaatgggca gctttttatg
    45181 gttcgttgga tatggaagcg agaaatagag tcgataaatt gattcagcca ttagtattaa
    45241 agggtcgtca agcagggatg tacattattt tagcaatgca acgaccagat gcagaatatt
    45301 ttccaagtgg tgttcgggat aatttaacat tccgtatttc tgttggtcga ttgtctccca
    45361 ttggttattt aatgactttt ggagaagatt ttaaaaataa gccattttac aacaaagatc
    45421 aacctggtcg tggatatgct tttccaggat ttgatgtacc tgtagagttt tttgcgccat
    45481 ttgtttacaa agattatcac tttatcaaag aatttgagaa atttactgaa atgattgtct
    45541 atgattttac agtaaaagag gatagtcaag atcaaacgat gggagatgat aatcagttgt
    45601 tacaagaaca actagagcgc caaatgtatt tagcaaaaga tccaaaagga acaagttttt
    45661 ctgatgcgca tattttgtaa tcaactaagg aggtgaagtt aaatggcaga gagttgttat
    45721 tatagagtag ctacagcaat acgaatgatt aatccttctc tttcttccag aacgttttat
    45781 gattggttga atcgtattga acaagtaact gattaccgct ttttgcgcaa agaacgtgtt
    45841 tttacaggaa aagttattaa tcaagtattg ctaacgaaaa aagatataga acgattgact
    45901 cgattgtatc attatcgagt agatttagaa gaggatttaa ctttgtcaat ttatcgcgtt
    45961 ttttctcctg aaaaatatag tgaaataaca aaattagatc atttaatttt gtagttatta
    46021 tctatcggga gggctctaga cgtagtctgg tagccgcact ctgcctggcg gtgcgcatta
    46081 aggctgcgaa gcatgggcct ataatgcgca ctcgctagca gcgtgtgcag ctcacaaaga
    46141 ggtcgtgcgc ttgcgcacgg cctctttgtg taaagccccc cgatatataa tatggggggc
    46201 agaaataaca taacacatag aaaaaagttt tataaaccct tttgtatcaa gtgattatct
    46261 aaggtcagca gttgtactac ttttcccaaa aaacgagggg gactagttga tatatcggtg
    46321 tttctaaagg gttaaaaaaa atcgattttg ttattttata aaaggagcga gaaaatgaca
    46381 ccgattgagc taaaaagcta cagaaagaag ttaagtgcaa ttcataatat aagtggagat
    46441 aaaaagccgc tatcacaagc aagatttgcg caattgatgg gctgctcaaa agattacatt
    46501 agaaagatgg aacaagggaa aagaaaaatc ccagaggatt ttgaacaacg aatcaaggaa
    46561 cgctttcgat tgtatatgaa aagcggaata gatcttcgag caatgattga ttatttgcgt
    46621 ctttcctttt ttgggacaga ctacaaaaca gtgattcaac gtgtattacg aatggatctg
    46681 acagaatttg aaacacgtcc aacgggcttg tatctttttg actgtctttc tgttcgtggg
    46741 aatatctggg tgttctggca ttcaaaacga actgtaacag aaaatgtact tgtgcaattt
    46801 tcaggacaag gttgccggga atatgaggaa gtcatgcgtg aacaacaaac agattggcga
    46861 acatggctat attccatttg gaaaaatcaa ggaaagttgg aagctggtta tacacgtgtg
    46921 caatgtagcc gattcgatat tgcgttagat gaactttggg gattggaaac ggaagaacat
    46981 ttcgatctct ttgaattact agaaaaacaa aaagcaggtt tattagaaat ggactttaag
    47041 agattccaga atattggcgg ttgtttttta gatgatggct attttcgaag tgaaggatta
    47101 tctttgtatt ttggatcgcg aaagagtcct ctctttttta atttttatga gaaacgtttt
    47161 gaaattgcga accgagaaaa aatttcagaa ttagaagcat taacgaaata tggtatttat
    47221 aatcgatatg agttacggtt agccaatgag aaagcaacac aagcagtaga agcatttatt
    47281 ttaggtgatt caccgaaacg tttaggagag attggcgttg gtttaattaa tgcaaaactt
    47341 attgtttggg atagactgga aagtgatgca cggattgttg ataaacgatg ggcaaaatta
    47401 tttcgttcga tggaagaatt ggattttaca gtaaagccag aaaaattctc attagatcgt
    47461 tcagaacgct ggtttttgaa acaagtagct ccgacattaa agttgttaca agaagcagat
    47521 cgtattttag gaagagactt tgtacaagaa gggattgata atgccgaact aaatgacgag
    47581 caacaaaact atctagaatg gatgggaacg tcaggaggga aaattgcatg aatgcatata
    47641 cgacaacaga atggctgcgc ctgttggatt taaaggctgc aatcgaagca ttaaatgaaa
    47701 agatggttga tttgtcttat tttcgttttc gagttcctta tattgaacaa gcggtaaaag
    47761 ctggacgtta tcaggaaaaa gagaactggc aagaaattgc acgcttatta gaagttcgta
    47821 agggatacga acaagaactc gaagagttgg aatttagccg aaggaaagga aggctagaat
    47881 tcattcgctt ttatcgattt tcattgccaa tacctgcgat tttagcggtt aaaaaaggct
    47941 gcgataaaat gaagatatat gaaaactgtg tggcagcact tagcagcgaa aaacctctta
    48001 tggaagaaat ttcattggtg actgtcactt gggaaatgag tcgccagcca acagaagaac
    48061 agacatatct ttctttagaa gagattgaga tagaattaga ggaaattggt cgctatgcca
    48121 cttgttctac ctattgtgga tcggtcatta gtattgctgg tgtgattgtc taaatgaaaa
    48181 aaattatgat ttatgttggt gcgtattggt cacgtgatcc aacagtatta gaaaatccag
    48241 aggcggtaca ctattgcttg cgccaattat tctatctgta taaagaacga ttagaatcgt
    48301 tgattcgtca gttaccatat actgacagac gtttagatga acttcttcta aggtatccag
    48361 caatgtataa acgacgaaaa aatagattgt taccagaaga gtatcccatt gaaaaacatg
    48421 aacttgaagg gcgatttgtg gcttatttct atgatgatgt tcgtatgcgc ttagtagaac
    48481 aacgaatgga aatagaaaat gatcgctact attttatcaa ttattgtaag aagcgtaagt
    48541 atcaagtaac tgatgatttt tatgattgta ttttacaaga tggggaagtg attctatcca
    48601 aaattgctcc aagttttcgt gaacttgtgc cgattgattt tttgaaaaaa tgtcatttgc
    48661 ggattcttcc gtaaaaaaga gctataaaag gaaagaaaag tagaagccaa atcggtttct
    48721 gcttttttat tttttttagg aaggagaaga tggtatgtca ctggaatttt taggagagcc
    48781 aattgaaaag gaagagttcg tttcgcatta tatggatttg tttattcagg tgataaaaaa
    48841 ctttgtgcga ttgatgaatt ttatccacgc gaacaagact atctagccgc tgaacgaata
    48901 aaaatcagtt tattatggga aaaattcaca gatgaaaata accgaccgcc agattatcgc
    48961 tatttatcag attgtttaac gaataatgtg ctttctaaag aatacctatt tcacaaacgt
    49021 gagaaaaatc aaatgtcggt tgaacatttt tgtgattgtt atattgcatt attgcgtagt
    49081 caacagttat taacagtaga acgatatggt gcagagtttg gttatgtcat gcaagctgag
    49141 cgtcatagag ctacaagatg ctatgaaaaa gaagatcaag tgattgatgg ctatgaagaa
    49201 ttatgtattc gaaacaatcg ctatttgcaa caacgtttat atgaacaatt gatgatggat
    49261 ttttcaaatc tctatagagg gtatgaatgt tcgatgattc aagaaaggta gaagaaaagg
    49321 gggaattttt atgtggcgat tattattttt ggtggtatgt ggtatggcat tgttctatgt
    49381 gtatcgtgtc tatctctatc cacgaatgga acgatggaca agttggtata gagatccttt
    49441 aaaacaacga aaaggattga agcggtatcg ttatgaatta cttgttgaat tttcggatgg
    49501 gcgacaagca cagtattatt tttttgcaaa ctatccaaaa tacaacacca gtgaactatt
    49561 gattcaatta ttcaaaaata atccatatgt tgggttagaa gaaaacggac gggagttctt
    49621 ttatcgttca gataatatta gtgcggcgtc tatgaacaaa acacgaattg cataaaaaat
    49681 tggaggaata atcatgaaaa aaacaacatt agcagtcgta acaacactag gaatttcatt
    49741 attttcgttt caattaggga gttcattggt ttatgcagat gaaactgtag aaacacctaa
    49801 gtcagaaatt gtagaaacat tgccagggga aggattacca gatgttgaga cagagctaaa
    49861 tggaggaaca acggaaaaac cagcagaacc tgaacaattg cagtcagaga aacctgaaaa
    49921 agagccagtg acaccagaac cagagtttcc tgaatcaact ttaccgactg attcgatcga
    49981 agaagaaggt gtgcatagtt caacaacaac ggattcaagt atagtcacaa ctccggaaca
    50041 atcaggaaca accagttctt ctgaaaagaa tactgattcg tcaagtacgg aacagccaac
    50101 gatgtcgtct accagtgaag aacaaaaaga agaaccgaaa caacctgttg aaaaatcaga
    50161 agcaattcca aaagaaaata aacctattgt tccagcaaaa gaagtcacag tatcggtaac
    50221 cccaagcggt gaaattacga cgaatacttc tcaaggaatg agtgtaccga ttgtcactag
    50281 taatgtagag gaattgaccc atattccaac accaaccacc ccattaaaag tagcaggtga
    50341 tcaaacaatt gttggtgtaa aagatggtat tccattaatc caagatagtg aagggaattt
    50401 ggtaaaggat ttgtcgattc ctgtaaagaa acttccaagt ggcaatattg aaatcaaaac
    50461 agcagatgga aaaacaaaag tcttaccaaa gactggtgaa gaaatacatg ttgcactttc
    50521 tgttctagga ggagtgttga caagtattgc tggatttgtt ggatataaaa gaaagagaaa
    50581 aggaatagaa aatgagggta attgaatgga ctatttttca gaaaaacaag cttatcgttg
    50641 tcaatcgtac gtactagtaa aaacatacta gaaggagtag cttatgaaaa aacgacaact
    50701 acggcggaaa gaaaaagttt atacagagaa agtaccgaag caaaagaaaa tccattcaaa
    50761 aaaagcagtg aaatggattt ggtgtttggt tttatttctt tgtttttcag ggggcttggc
    50821 attttttcaa tccatgaaaa cgaaggttca agtgtcgttt ttgaaagatg aaatccaaca
    50881 attacaaaag aaagaagtac aaagtcggtc agaattaacg tacacacctg aaattgaatc
    50941 ctttatgaat cgttttgttg ctttgtatat gaatacattc gataatcatg aacaagaaca
    51001 acaacgtcaa gaaacattaa aaaaagagtt ttatgcacaa aactttattc aagaaccagg
    51061 aagtgtttca ggtgttcgaa aattaactgc tgcttcgttt attggattaa aagaaattga
    51121 aaccattcca actgctagtt ataaagtaac ttatgatatt cgctcgatga atccagagag
    51181 taagaaagaa acaagtgtga caatcacaca agtactcaat cttccctttt ctatttcaaa
    51241 caagggctgt cgtattattg cttatcctta ttttacggca ctaccaaaga atacagaaaa
    51301 acaacgaatg attacttatg atgacacatt atacgaaacg gttgatgcgt cggaagcgaa
    51361 agatgttgaa ggatttgtgg aagatttctt agataagtat gcgaaaaaca atgcaaaaga
    51421 tatgtcatac atgatgaaag aacctgaagg actcaatcaa acagctaaag ttgatcaagt
    51481 agagatgaaa gtattgcgtt ataaagaaac gaaaaaacct tttattgtaa aagcaacggt
    51541 cgtctttcga gatggtgata cgtcactttt acaaaaagaa caaatgacga ttgtcattac
    51601 gaaaaaaaca aatcagtatt atgttgaaaa attaacacat aattggttgg ataggaaagg
    51661 agaagaacgg aaatgacact gaagggaatt gtagatttta tattaacgca aggtggctat
    51721 tttttaataa tggtggttgt ttatctggga ttgcgaaatt ttatgaaatc aaaagtagga
    51781 aatgcattaa tatctatcgc agcaggtgcc gcagtttatt tcttcttaaa tgatccaaca
    51841 cgtgtatttg atgcgtttgg taaaatcatt gaaaaattat ttatggatta ggaggatagt
    51901 gaagtggatt gggaacctta tgattattcg attgtttatg cggaacctta tcgcgtacaa
    51961 cagattacaa gcaattggcg tcttccattt ccagttgcat ta
//
LOCUS       DAPBCS010000016        47862 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPBCS010000016 DAPBCS010000000
VERSION     DAPBCS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.016"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(37..1188)
                     /locus_tag="RD521_001119"
     CDS             complement(37..1188)
                     /locus_tag="RD521_001119"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDT7770867.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="RD521_001120"
     CDS             complement(1201..1638)
                     /locus_tag="RD521_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7770868.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="RD521_001121"
     CDS             complement(1653..3419)
                     /locus_tag="RD521_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDT7770869.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="RD521_001122"
     CDS             3683..4360
                     /locus_tag="RD521_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770870.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="RD521_001123"
     CDS             4363..5490
                     /locus_tag="RD521_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770871.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="RD521_001124"
     CDS             complement(5690..6130)
                     /locus_tag="RD521_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7770872.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="RD521_001125"
     CDS             complement(6141..8174)
                     /locus_tag="RD521_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7770873.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="RD521_001126"
     CDS             complement(8176..9879)
                     /locus_tag="RD521_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7770874.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="RD521_001127"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="RD521_001127"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDT7770875.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="RD521_001128"
     CDS             complement(10962..11987)
                     /locus_tag="RD521_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDT7770876.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="RD521_001129"
     CDS             complement(12196..13065)
                     /locus_tag="RD521_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDT7770877.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="RD521_001130"
     CDS             complement(13524..15416)
                     /locus_tag="RD521_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770878.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="RD521_001131"
     CDS             complement(15413..17140)
                     /locus_tag="RD521_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770879.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="RD521_001132"
     CDS             complement(17156..17806)
                     /locus_tag="RD521_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDT7770880.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="RD521_001133"
     CDS             18010..18306
                     /locus_tag="RD521_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDT7770881.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="RD521_001134"
     CDS             18478..19098
                     /locus_tag="RD521_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDT7770882.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="RD521_001135"
     CDS             complement(19190..20356)
                     /locus_tag="RD521_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7770883.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="RD521_001136"
     CDS             complement(20368..20721)
                     /locus_tag="RD521_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDT7770884.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="RD521_001137"
     CDS             20849..21871
                     /locus_tag="RD521_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDT7770885.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="RD521_001138"
     CDS             21890..22051
                     /locus_tag="RD521_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770886.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="RD521_001139"
     CDS             complement(22310..22744)
                     /locus_tag="RD521_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770887.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="RD521_001140"
     CDS             complement(22854..24713)
                     /locus_tag="RD521_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7770888.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="RD521_001141"
     CDS             complement(24831..25991)
                     /locus_tag="RD521_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDT7770889.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="RD521_001142"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="RD521_001142"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDT7770890.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="RD521_001143"
     CDS             complement(27738..28436)
                     /locus_tag="RD521_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDT7770891.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="RD521_001144"
     CDS             complement(28393..30054)
                     /locus_tag="RD521_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDT7770892.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="RD521_001145"
     CDS             30272..31672
                     /locus_tag="RD521_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDT7770893.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="RD521_001146"
     CDS             complement(31924..33003)
                     /locus_tag="RD521_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7770894.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="RD521_001147"
     CDS             complement(33089..34048)
                     /locus_tag="RD521_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDT7770895.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="RD521_001148"
     CDS             complement(34060..35571)
                     /locus_tag="RD521_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDT7770896.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="RD521_001149"
     CDS             complement(35593..36156)
                     /locus_tag="RD521_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDT7770897.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="RD521_001150"
     CDS             complement(36162..37025)
                     /locus_tag="RD521_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7770898.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="RD521_001151"
     CDS             complement(37039..37935)
                     /locus_tag="RD521_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7770899.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="RD521_001152"
     CDS             complement(38110..39399)
                     /locus_tag="RD521_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7770900.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="RD521_001153"
     CDS             complement(39371..40888)
                     /locus_tag="RD521_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDT7770901.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="RD521_001154"
     CDS             41086..41976
                     /locus_tag="RD521_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDT7770902.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="RD521_001155"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="RD521_001155"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDT7770903.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="RD521_001156"
     CDS             complement(42885..44357)
                     /locus_tag="RD521_001156"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDT7770904.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="RD521_001157"
     CDS             complement(44994..45356)
                     /locus_tag="RD521_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDT7770905.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="RD521_001158"
     CDS             45579..46331
                     /locus_tag="RD521_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7770906.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="RD521_001159"
     CDS             complement(46471..47394)
                     /locus_tag="RD521_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7770907.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="RD521_001160"
     CDS             complement(47409..47681)
                     /locus_tag="RD521_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770908.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13932 a         9945 c         8260 g        15725 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt ac
//
LOCUS       DAPBCS010000017        47435 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPBCS010000017 DAPBCS010000000
VERSION     DAPBCS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.017"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(20..754)
                     /locus_tag="RD521_001161"
     CDS             complement(20..754)
                     /locus_tag="RD521_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDT7770909.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1081..1491
                     /locus_tag="RD521_001162"
     CDS             1081..1491
                     /locus_tag="RD521_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDT7770910.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1484..2185
                     /locus_tag="RD521_001163"
     CDS             1484..2185
                     /locus_tag="RD521_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDT7770911.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2185..3771
                     /locus_tag="RD521_001164"
     CDS             2185..3771
                     /locus_tag="RD521_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770912.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3790..4011
                     /locus_tag="RD521_001165"
     CDS             3790..4011
                     /locus_tag="RD521_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDT7770913.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4008..4769
                     /locus_tag="RD521_001166"
     CDS             4008..4769
                     /locus_tag="RD521_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDT7770914.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5149..5658
                     /locus_tag="RD521_001167"
     CDS             5149..5658
                     /locus_tag="RD521_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDT7770915.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5733..6272)
                     /locus_tag="RD521_001168"
     CDS             complement(5733..6272)
                     /locus_tag="RD521_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDT7770916.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6412..7023)
                     /gene="rpsD"
                     /locus_tag="RD521_001169"
     CDS             complement(6412..7023)
                     /gene="rpsD"
                     /locus_tag="RD521_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDT7770917.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7285..8136
                     /locus_tag="RD521_001170"
     CDS             7285..8136
                     /locus_tag="RD521_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDT7770918.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8164..8799
                     /locus_tag="RD521_001171"
     CDS             8164..8799
                     /locus_tag="RD521_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDT7770919.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8819..9592
                     /locus_tag="RD521_001172"
     CDS             8819..9592
                     /locus_tag="RD521_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7770920.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10000..10797
                     /locus_tag="RD521_001173"
     CDS             10000..10797
                     /locus_tag="RD521_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDT7770921.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10775..11188
                     /locus_tag="RD521_001174"
     CDS             10775..11188
                     /locus_tag="RD521_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7770922.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11172..13817
                     /gene="mutS"
                     /locus_tag="RD521_001175"
     CDS             11172..13817
                     /gene="mutS"
                     /locus_tag="RD521_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDT7770923.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISSVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13835..15943
                     /gene="mutL"
                     /locus_tag="RD521_001176"
     CDS             13835..15943
                     /gene="mutL"
                     /locus_tag="RD521_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDT7770924.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15965..16525
                     /gene="maf"
                     /locus_tag="RD521_001177"
     CDS             15965..16525
                     /gene="maf"
                     /locus_tag="RD521_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDT7770925.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16610..18598)
                     /gene="treP"
                     /locus_tag="RD521_001178"
     CDS             complement(16610..18598)
                     /gene="treP"
                     /locus_tag="RD521_001178"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDT7770926.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18821..19534
                     /gene="treR"
                     /locus_tag="RD521_001179"
     CDS             18821..19534
                     /gene="treR"
                     /locus_tag="RD521_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDT7770927.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19611..20057
                     /gene="msrB"
                     /locus_tag="RD521_001180"
     CDS             19611..20057
                     /gene="msrB"
                     /locus_tag="RD521_001180"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDT7770928.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATEQPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20227..20829
                     /gene="ruvA"
                     /locus_tag="RD521_001181"
     CDS             20227..20829
                     /gene="ruvA"
                     /locus_tag="RD521_001181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDT7770929.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNEKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20842..21843
                     /gene="ruvB"
                     /locus_tag="RD521_001182"
     CDS             20842..21843
                     /gene="ruvB"
                     /locus_tag="RD521_001182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDT7770930.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21872..22450
                     /locus_tag="RD521_001183"
     CDS             21872..22450
                     /locus_tag="RD521_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7770931.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22502..22984
                     /locus_tag="RD521_001184"
     CDS             22502..22984
                     /locus_tag="RD521_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDT7770932.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23101..23475
                     /locus_tag="RD521_001185"
     CDS             23101..23475
                     /locus_tag="RD521_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770933.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24153..25505)
                     /gene="brnQ"
                     /locus_tag="RD521_001186"
     CDS             complement(24153..25505)
                     /gene="brnQ"
                     /locus_tag="RD521_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDT7770934.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25652..26242
                     /locus_tag="RD521_001187"
     CDS             25652..26242
                     /locus_tag="RD521_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDT7770935.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26370..27719)
                     /locus_tag="RD521_001188"
     CDS             complement(26370..27719)
                     /locus_tag="RD521_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7770936.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27887..28627)
                     /locus_tag="RD521_001189"
     CDS             complement(27887..28627)
                     /locus_tag="RD521_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7770937.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28640..30232)
                     /locus_tag="RD521_001190"
     CDS             complement(28640..30232)
                     /locus_tag="RD521_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7770938.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAVLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30800..31096)
                     /locus_tag="RD521_001191"
     CDS             complement(30800..31096)
                     /locus_tag="RD521_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770939.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31272..31997
                     /locus_tag="RD521_001192"
     CDS             31272..31997
                     /locus_tag="RD521_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDT7770940.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            31990..32832
                     /locus_tag="RD521_001193"
     CDS             31990..32832
                     /locus_tag="RD521_001193"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDT7770941.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32835..33356
                     /locus_tag="RD521_001194"
     CDS             32835..33356
                     /locus_tag="RD521_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDT7770942.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33609..34172)
                     /locus_tag="RD521_001195"
     CDS             complement(33609..34172)
                     /locus_tag="RD521_001195"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDT7770943.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34423..34692
                     /gene="rpsO"
                     /locus_tag="RD521_001196"
     CDS             34423..34692
                     /gene="rpsO"
                     /locus_tag="RD521_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDT7770944.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34880..37006
                     /gene="pnp"
                     /locus_tag="RD521_001197"
     CDS             34880..37006
                     /gene="pnp"
                     /locus_tag="RD521_001197"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDT7770945.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37516..37782
                     /locus_tag="RD521_001198"
     CDS             37516..37782
                     /locus_tag="RD521_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDT7770946.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37959..39917
                     /locus_tag="RD521_001199"
     CDS             37959..39917
                     /locus_tag="RD521_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDT7770947.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40271..41572
                     /locus_tag="RD521_001200"
     CDS             40271..41572
                     /locus_tag="RD521_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDT7770948.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41702..43072)
                     /locus_tag="RD521_001201"
     CDS             complement(41702..43072)
                     /locus_tag="RD521_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDT7770949.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43254..43577)
                     /locus_tag="RD521_001202"
     CDS             complement(43254..43577)
                     /locus_tag="RD521_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDT7770950.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43902..44510)
                     /locus_tag="RD521_001203"
     CDS             complement(43902..44510)
                     /locus_tag="RD521_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDT7770951.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44840..45556
                     /locus_tag="RD521_001204"
     CDS             44840..45556
                     /locus_tag="RD521_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDT7770952.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45569..46264
                     /locus_tag="RD521_001205"
     CDS             45569..46264
                     /locus_tag="RD521_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDT7770953.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46348..46977
                     /gene="udk"
                     /locus_tag="RD521_001206"
     CDS             46348..46977
                     /gene="udk"
                     /locus_tag="RD521_001206"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDT7770954.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15379 a         8780 c         9871 g        13405 t
ORIGIN      
        1 ttttttattt agcttttgat taagccataa ataataattt atataagaat actgctacga
       61 caccagcaca gataggtgct acaactggta cccatgcata tccccattga gaagaacctt
      121 tatgtttcaa tggaagaagt gcgtgtacaa gacgtggagc aaagtcgcgg gctgggttta
      181 gtccaggacc tgtaggacca ccaagtgaag caactaatgt ccatacaagg aaaccaagtg
      241 ctaagtgagc tgtaccaaga ttatcttgga agaatggtgc ttttgtaata cctagggcac
      301 caaagaataa aacgaatgaa ccaaagaatt cattgacgaa accattgaat ttagaaccaa
      361 cgctgttgat tgttgagaat gtacctaaca catggtttac gtcttctgtt tggtcgtagt
      421 aaggtttgta acaagctact acgatcaatt gtccaatcgc tgcaccaatc aattgtgcaa
      481 tgatataagg aacaacttca gcccaaggga atagaccatt agatgctaaa gcaattgtaa
      541 atgctgggtt gatgtggtta ccgctgattc caccaaaaat cattgctggc atcataaccc
      601 cgaatccata cccgacagca atcagcatcc agtcactgcc atatcctttt gtacctttta
      661 aatctacgtt tgctacagca ccgttaccta gaacgattag taatgctgta ccaatcattt
      721 ccgccgcaag acgggtcatt agtgagtaat ccataaatac ctccaaaagt tataaaaatg
      781 caaaaaaaaa agaaacatca acacgaaaag catcggtgca gtgtttcgtg tcggaaggat
      841 ttctgcagtc ttaacaaatg tgaataaaaa aatgaattcc attacttata agaaaccaga
      901 ccaacagtaa aactccaact ctttttaaaa gttctccaca aatcaccaag cttaactata
      961 aatataccaa aaaaaaaacg ttttttttct caaaaattgt atcttagaaa cagcatgttt
     1021 tgaaactttc agaaaaggtt tgttagtaaa atgaaagcta tacgtaagga gtgttgtttg
     1081 atgaaatttt ttcaagagtt tttcattatt attttctttt ctgtattagg agaaggcata
     1141 cggatttttg ctcatttacc tattcctgga agtatcttag gtattctttt tttattttta
     1201 gcatttgaaa tgcatcttgt tgatcctgaa aaaatcggag caactggtga ttttttattg
     1261 aataatctag ccattttatt tgttcctgct ggtgtaggtt tattggaata ttttgatgat
     1321 attgcttcga tatggcctgt tttactggga gcagtcgtcg tgtgttccat cgtcacgatg
     1381 gttgcagcgg gaaaaacggc agaaggtgta gaagctttgc tgggctatgt ccgtaagaaa
     1441 aaagcagaaa gggtatctgt aaacgaaatg gaggagaatc aaattgactg agttttttgt
     1501 gaatccatta tttggcttaa gtttgacttt aggagtgtac gtattatctg acaaattttt
     1561 gcggaaatat aaactgcctg ttttgaatcc tctcgtatta tcgattgttg ttattatctt
     1621 atttttgcat ttttctggga tcagctataa agactataat atcgggggca attttttgac
     1681 tatgctgatt actcctgcga cagtggcact tgccatccct ctttataaaa acttccgttt
     1741 actgaaagag aatttttatc ctgtaatggc tgcgatttta gcaggaatag tcgcaaattg
     1801 cttggttagt gtagggttcg gttacttctt ctctcttcat aaagagatgg tgatctcatt
     1861 actacctaaa tctgtaacga cggctatttc tgtcgattta tctcatacga tgggcggggt
     1921 gaatgccgtg acacttgcta ttgttgtctc tacaggtatt tttggttcgc tgatcgccac
     1981 tcatattttc cgcctattca agatcgaaag tccagttgct cgcggtgtgg cgttaggaag
     2041 tacttctcat gctattggta cagcaaaagc aatcgagatt ggtgaaatag aaggaatcat
     2101 ttccggactt gcgatatgcg tcaacggaat attgaccgtt ttattgctac cgttattctt
     2161 tcagcctttt gctggcttgt tttgatgcga aaaccactat tttatgctac aataaccaaa
     2221 acgttcgagc ataaaaaagg agggaaagac gtgtatttgt cttccgagta tcttaaacaa
     2281 tttgataaag atctatatta taagattctc ctcctagaaa gttttgagga tcaagcttgg
     2341 cataccgctg cacaattagc gcaagttgtt cagctggatg cccgcagcgt gtcgaaatac
     2401 ctgaacgagc tgtctaaaaa ctatcagcag tttagcggta agacacaccc tttgtttaca
     2461 aaaaatcacc gttctggtta taacttttat gatacgctcg attcaattga acatgagcgt
     2521 tttttgatct atctcgtgca aagtacgctg aaattccagt tgctgcacga catttttttt
     2581 gaagaattcc atacaatgta ccaatttgcg caaaagcatt acattagtga atctactgct
     2641 catcgaaaaa tcaatgaatg gaaacagcaa ctgcaaactt acggaattcg gctgcaacga
     2701 ggtacatata tcgctcaagg cgaagaagaa atcattcgac tttacttgca tatgacattc
     2761 tggcagttgt ttcgagggaa aatctggcca tttgaaacga tttctcaaat ggacgtaaag
     2821 aatatggcag aacatatcat ggcttttttt aatgttcgtc tgaacgagat caaaaaaaga
     2881 cgattggaat atatgctagg tgcctttttt ttaagaaaat cacagaagca ttatgttgta
     2941 ttgaatgaga aaaaaagacg gctgatttct gacaatctgt tgttccagcg attttgccag
     3001 gtcatggagc cggtatttcc taattatttt caagtagaag acgagttagg cgcattattt
     3061 cttgtactga tgactcgtga agaatattat agtgatccta agatacggaa gaaaattttt
     3121 gattttcatc aacaagcaaa aacaccgccc tttacagccc tttcagaagc aaaagccgct
     3181 ctttctcttt atcaggaaga gcaggggctg ccagcagaaa atctaacctt cgaggcagaa
     3241 aactatttat tcagcagtca ttttttcgcc tatctttttc ctaatgcgaa agaaacgatt
     3301 gatggcaata gtagtgattt tattaatcat ttagttattg aaaacaagga attgaaacaa
     3361 tggctcgtac atttttttga aagtagacac aaacatccca atcatctggc atttaaaaat
     3421 catgcgtttt tgatggggcg gtatctgact gtgtttaaaa cattaggagc ctttactcca
     3481 cagcttccaa agatcacgat ccttttaatg acagatttcc cattatttga agagcaactg
     3541 ttagaggagg gattacggac atttttccgt aatgagtatc agctgatttt tctgccaaca
     3601 gattatcggg ggagagaagt tgatcttctg atttcgacaa gtaaagtcca ccgcaaacca
     3661 tgggcggatc ttgactattt tattgtgact gaagaactaa aattaattga ttacatccaa
     3721 ctatctcaaa aatttgaaat gattcaaaaa caaaaactat taaaacaata acaaagaagg
     3781 agaaaaaaga tgacatttga agaaatttta ccagaattga aaaaaggtgc aaagattatt
     3841 cgtaaaggat ggagtggatt tgaactttat gttgttctcg tatccgatga tgaatttgat
     3901 ggctgtccgg tgacgccgta cttattgatc aaaacaagtg acgaaggttt ttccagcttc
     3961 gctcctacag tttgtgatat tttagcagat gattgggcga tcgtagaatg aaaaaccagt
     4021 atccagatct agctaaacag gttgtattta tcacaggagc tgcttctgga attggtgctg
     4081 cgcaagcccg agcgtttctt gaacaagatg cttttgtatt tggattagat caaaagttcg
     4141 gtaaaatgga agaaatttct ttgcgatttc ctgaaacttt tgcctacgca ctaggtgatg
     4201 ttcgacaaat gaaagaactc caacaagcgg tatcgaaatg ccaaaatcat tttggtgaag
     4261 ttacgatttt actgaataca gcggggatat tggatgccta caaacccttg atggaaactg
     4321 acgaatcgct gtgggatctg atttatgaga cgaatgtgaa aagcatgtat cagctgacaa
     4381 aaatcatcct tcctgatatg atccgccaaa aaagcggaac gatcatcaat atggcttcga
     4441 ttgctggctt gattgctggt ggaggtggga tcgcttatac aagcgcgaaa catgcgattg
     4501 tcggatttac caaacaactc gctctggatt acgcaggaca gggtatccga gtaaaaggaa
     4561 ttgctccagg agccatccaa acaccaatga atgcggctga ttttgaaggg aaaggtgaga
     4621 tggcagagtg ggtggcaaat gaaacaccag tcaagcgatg ggcacagccg gaagaagtag
     4681 ctgagctgac gttatttctt gcctctcctc aagcaagcta catacaagga acgatcatcc
     4741 caatcgacgg cggatggttg ttaaaataac cttgtgtttt tttcaagctt ctgtatgata
     4801 aaactaaaga gagaaacaac ggttgatgat aaaaaaagtc gatgactgtt cccgacattc
     4861 gatgccgaac agccagttca aaaaattctt tttaataatc gttaatattc gaaactaagc
     4921 ggcttttcac gacctcttga tataaacgat attcaaaagc ttgcaactgc ggtaacaagt
     4981 ccagccaaat gaaaaatata aggagctatt tttatttgac cattcttatt acttgttgca
     5041 gggcagcttt ttcactacct tctgcgacac ttcagttgga attttcttcc catcctttgg
     5101 tcgcggtcat taccaagata aaattatctt gcaaaggaga aatagaacat ggaaaaaaat
     5161 aatacccgtt cagccaaacg ctggacaacg acagatgtga ctcggatggc gctagtcact
     5221 ggtttatatg tggcagtgac gctggttttg tctgtcttta gctttggagt gattcagatt
     5281 cgtctctcgg agatgttcaa ctatctgtcc ttgtataaca aaagatacat cgtcgcggta
     5341 acagcgggag tagctttggc taatattgct tctcctcttg gactgataga tgttgcagta
     5401 ggaagcgtca gcacgctgct tgtattgctt atcaactata aaatcactag tcgcatcaaa
     5461 aacatgaaaa tcaaaatggc cgtgacagca tgcgtatttg ctttttccat gtttacagta
     5521 gctggtcagc taacgatttt atatcaacta ccttttttct tgaattggtg ggtgattgct
     5581 ttaggagaat tggcatcaat gattgtcggt gggataatta tttattggat cggtaaaaaa
     5641 gtggatttgt caaaataaag atctgtttaa tttaaaatag ggcagcatgg aagagactga
     5701 atgttgagtc ttcttccatg ctgctttttt gattaatttg tatgaagaaa tcgatttagc
     5761 cgttttttca tcaagatacc gatataagct gctccagttg ccttgattgc ttccggcagt
     5821 aaaaacggca gtagtccact ggcaaatgca gttgtaaaag taagatcacc acttattttc
     5881 agccagatcg ttccgcagat caatgcagca agagagccca ttagattggc aaagattgca
     5941 tataagtaat gagcatccgt tctttcgatc atccaaccag ttatccacgt ttgtaagaga
     6001 aatccaatca aatatccgcc agttggccca aataaaacag ctatacctcc agtcatccct
     6061 gaaaatacag gaagaccaat caagccaagt aaaagataaa ctccaacaga tatcaatcct
     6121 agttttcttc cgagaaaagt tgctgttaat ccaatagcaa atgtttgccc tgtcaaagga
     6181 accaaaccaa aaggaatggt catctgagaa aggacagaaa taatcgcggc aaattccgca
     6241 gcaagtatca attctttcaa tgttcggttc ataacgccct cttttcgtta acttaatatt
     6301 tttatttggt taacgaaatt gtaaaacaaa gtgattactt tgtaaataga aaatataaaa
     6361 aaagaccagc tatccaatag atagcggtct ttttcaagaa agcttttgaa attataagct
     6421 ttggttgtag tattcaacga caagagcttc gtcgatttct ggatatagtt cgtcacgttc
     6481 tggtaaacga gtcaatgaac cttccaattt ttcagcgtca aagcttacga atgctgggcg
     6541 tccaacagtt gcttctacag cttcttttac tgttacgatg ttttgtgatt tttcacgaac
     6601 tgagatcact tgacccactt ctacgtggta tgaagggata tcaacgcgtt tgccatcaac
     6661 taaaatgtga ccatggttaa ctaattgacg tgcttgacga cgagtcgttg caagtcctaa
     6721 acggtaaaca acattgtcta aacgttgttc tagtaagatc atgaagttaa caccatgttt
     6781 accttctttg attttgctag cttttacgaa caagttacgg aattgacgtt cgttcattcc
     6841 gtacatgtga cgaagttttt gtttttcagt taattgcatg ccgtattctg attgtttgcc
     6901 acggctatta ggtccatgtt gacctggtgc gtaagggcga cgtgctaatt cttttcctgt
     6961 gcctgacaat gagatgccta agcgacgaga aattttccat gatggtcctg tataacgaga
     7021 cattaaaatt tcctccaatg aatataagtt tttggagtaa aataatccgt tgaaaaattc
     7081 ataatcatat agtttgttct tcaatcttca cctttgcagc cgcggttaca caatcgaacc
     7141 cgagacgggg caacaaacta gggatgagtt attatttttc tgctgcatta ttttacacaa
     7201 attacagtat atgatagatc atccacgaat gtcaagataa ttagtacata aaaacgcttt
     7261 acaaacctag gctgtcttta taaaatgaga gggtatgtgc aaatggaaaa aagcgaagga
     7321 agtttaagga gaaaatcaat gaatccggta tcaccattat ttgagaaaat cgatgcttca
     7381 attgaaaaga aggcgaatct ttttgaaaac agcttttcac gttacgctgt tcgagcggtt
     7441 ctcgcctgtt tgtttttgac tttaggaacg gctgtggcat ttgcgatcgc gatgaagggg
     7501 gaagatatcg cgcatggtct aggaaagatt ttatacgcat ttatgttcag ctggggtctg
     7561 gtcatgattc tatacatgaa cgccgaatta ggaacatcga atatgcttta tatgaccgtg
     7621 ggtgtttttc ggcagagact tgctttaagt aaggcagcca agattctatt tgcctgtatt
     7681 tttttcaata tgattggcgg tatattatgt ggatttctaa tttctatgac tacgccattt
     7741 caagatttaa cgttggacaa ctttatgtta gaatcaatag cagtaaaact agcaaaacct
     7801 tcagcaacga ttttggtgga agggatgttt gcgaatatcg ttgtcaatac cgctgtactg
     7861 atcagcatgc ggatgaaaga ggatgtcgga aaagtgatca cagtcgtatt catcattttc
     7921 atttttgctt ttttaggata tgaacacgtg atagcgaatt ttcctgcctt tacgcttact
     7981 tactttgctt ctcacggaca gatggatgga atgacagtca gcaatgtgtt acataatttg
     8041 ttttttgctt tggcaggaaa ttatattggt ggcggtttag taatgggact gggatatgct
     8101 tggctgaatc agtcaaaatc atcctatgtg gactaaaaag tcgttcatta aaggggaagt
     8161 gccatggaag ttttagtttt tccaaaaaat gaggaaaaag cgttacaaat ggaaaagtac
     8221 atgaaaaatc ttttttcctt tgctggagta cctaagccgg aacgcgctct tttacaacaa
     8281 ccgttactgg atgagagcaa aaatttatct atttctgaag ttttagcttt tgttcattat
     8341 tattatgaac aaatcgaaag agagtatcag tatgtagcga tcgatttagt ccagaaaaac
     8401 atcaaacggc ttacatttga agacttagtc gtattgttgc cactagttca gaaaaaagaa
     8461 tggtgggaca gcatcgactc ttggagaaaa gtatacagca catggtgtaa agcctaccca
     8521 gagcagcgtg aagaagtctt tcaattgttt gaaaaaaagg aagatttttg gctacgtcga
     8581 atagcaatca cgttgcaatt aggttataaa cagcaaacag acacaggact attgatccga
     8641 gcaatagacg aagatcgtca aacaaaggaa ttctttatcc aaaaagcaat tggttgggca
     8701 ttacgcgatt atagtaagac cgatgccgat tgggtaaaaa gacagctgac aacagatttg
     8761 agtaagttag ctgttcgaga ggcaagtaag tatttataag aaagcgagac gacaagagat
     8821 gagacgcaaa ttatttgctt ttgatattga tggaacgttg ttagattcaa acaaacaagc
     8881 tttagatagc acaagagaag cattggaaaa attacgcaaa gccggtcatc tggtcacgat
     8941 tgctacaggg cggagccgat ttcatgcgca agagatcatt cgcgatcttg attttacgaa
     9001 ctacatttta tgtaatggag cggccggttt tttagaccat gaacaagtat ataagaattt
     9061 actagatcgc gaccagttgg atgcgttcgt tcaagaagcg catcatgagc agatcgatac
     9121 agcttttgtc agcttagaca atatcaagcg ttcttcttcc aatgatgtaa cggtgatgga
     9181 agaagcgatg cattctttcg gtgctgtctt gcctgaatta gatcagtttt ttgcagagga
     9241 acaagaaata taccaagcgt tagcattttt cgatcaaaca tatgatggga agttctcttc
     9301 atatgataaa attcgatttg tccgctggca tgagaacagt gtagatgtcg tacctaaaaa
     9361 tggttctaaa gcagcgacaa ttctgcactt agcagaacaa gtaggcatcc ctcatgaaga
     9421 catcatcagc tttggcgatg gacaaaacga ccgtgaaatg ttgcgcatgt caggaattgg
     9481 gatagctatg gggaatgctg tgccagaagt acaagcagaa gctaagatgg tcactgatac
     9541 aaacgatcag gatggaattt ggaaggcatt gaaagagtta agcgtgattt aaaagaggtt
     9601 gtgaaaaagc tgttctgcat caagtaataa gcccagccaa acaaaaatag ctcctcctat
     9661 ttttcgtttt tctgggctta ttaccgcaga tgcaagcttt cgaacaccgt ttaaggaaga
     9721 ggtcgtgaca gaagtggtca gtttcaagaa ataagaaggg atttacgaaa attgcttctc
     9781 aaatttttgt aaaattacgg cttatttctg aagaagtaga tgaaacaacg tttattcggg
     9841 aaatgggact gtgacaagat gtttgtcata gtcccatttt tttattttat ttttcaaaac
     9901 taagaaatac caggaaagag ttccttttgt ttgacctttt ctttcttatt gaaaacaact
     9961 ataatataga gtgatgttaa aagagaagag gaatttagtg tgcgggtatt gtttattggt
    10021 gatgtagtag gttcaatggg acgagagatg attactgaat atcttcctag attgaagaag
    10081 aagtaccgtc cgcaagtaac gattgtaaat ggtgagaatg ccgcttctgg acgtggaatc
    10141 acagaaaaaa tctataaaaa atttttgcaa gacggcgtag atgtcgtgac gatggggaat
    10201 catacgtggg acaaccgcgg aatctttgag tttgtaaatg aagctaaaaa gatggttcgt
    10261 ccggcaaatt ttccggaagg gacgcctgga caagggatgg tctttgtcaa agtgaaccaa
    10321 gtcgaactgg cagtgatcaa catgcaggct cgttctttta tggtggactt ggatgatcct
    10381 tttcgtaaaa tgaatgagct ggtagaagaa gccagaaaac gtacgccgat catttttgta
    10441 gattttcatg gagaaacaac aagtgaaaaa caagcaatgg gctggttttt agatgggaaa
    10501 gtttcagcag tggtcggtac acatacacac gttcagacaa atgatgcgcg aattttgcca
    10561 agaggaacag cgtatctgac agacgttggg atgacaggtc cctatgatgg tattttgggt
    10621 atgcgcagag aacctgtaat cgaaaagttt ctgactgcat taccaaaacg ttttgaagtt
    10681 gtggaacaag gccgatcgat tttaagcggc tgtatcttgg aattggatga tacgacaggt
    10741 catgcaaaag aaatccagct gattcagatc aacgatgatc gaccgttcat ggagtagaaa
    10801 gagaggcagt tttttgcagc aggagccgga aattcaaaaa gaagtaagga aactgacaaa
    10861 acttttaaga gagaatgaaa cgatcattcg ctacaaagaa cttgaagaaa agatccagca
    10921 aaaccagtat ttagctgagt tgagagaaaa aatcaaacaa gcgcaaaaag atgctgttca
    10981 ttttgcccac tatgacaaac cagcagcaga aaaagaagca atcaaacaag cggatcaatt
    11041 catgcaagaa tttgatcagc atccgcttgt tgtagcttat agaaaacagc tgttggaagc
    11101 agatgatctg cttcatcatt taacaactat gatacaagaa gaaatcaatg gacaaataga
    11161 ggaggaaaaa catgcctcaa aaaactaaaa atactcctat gatggaacaa tatctaagca
    11221 tcaaggccca ataccaagat gcttttttgt tctatcgttt aggagacttt tatgaactat
    11281 tttacgaaga tgcgatcaaa gcgtcacagc ttttggaatt aacgttgacg agcagaaatc
    11341 gaaatgcaga agaacctatt cctatgtgcg gtgtacctca ccatgcagca caaggatata
    11401 tcgatgcctt gattgaaaaa ggctataaag tagcgatttg cgagcaggta gaagatccaa
    11461 agacgactaa agggatggtc aagcgcgaag tgatccagct agtcactcct ggtacagtca
    11521 tggacagcaa aggattgtcg gcaaaagaca acaatttttt aacagctgta gttagtcaag
    11581 gaaatgaatt tggctttgct tatgccgatt taagtacggg agaattgaag acggctcggt
    11641 tgcatgatga agaagctgtt ttaaatgaag cctctgcatt gcagacaaaa gaactcgtat
    11701 taggcagtga gatcactgat acccttcgtg aacagctaag cgggcgttta gggctagtct
    11761 tttctcaaca aaatgagatg gaagaaaacg cggaatttag ctttttgaca agcgaactag
    11821 tcgatccgct ggaaaaagaa gcaaccggca aactattgac ttatttatcg gtgacacaaa
    11881 agcggagttt agctcatatc caaaaagcag tagaatacca accagatcat tttttgaaaa
    11941 tggattatta ttcaaaattc aacctagaat taagccaatc tattcggact gggaaaaagc
    12001 acggcactct tctatggttg ttagatgaaa caaaaacagc aatgggtggc cgtttactaa
    12061 aacaatggct agaccgtccg cttatccagg caaaacaaat taaagcaaga caagaaatgg
    12121 tcgcttcttt gctagacagc tattttgaac gtatcgattt gcagtcagca ttaacgaaag
    12181 tctatgatct ggaacgccta gcaggtcgag tagctttcgg taatgtcaat gggcgagact
    12241 tgatccagct gaaaacttct ttagaacaag tgccaatgat cagagggttg attgaaggaa
    12301 tcgatcaagg tcaatggcaa agtctgcttt cagaaatgca gccgatggac catctcgtcc
    12361 aattgatcga ccaagctatc caagatgatc cgccgttaca aataacggaa ggaaacatca
    12421 tcaaagatgg attcaatgag cagctcgata tgtaccgttc tgctatgaga aacggaaaga
    12481 aatggttggc agaacttgaa gcaaaagagc gtcaagaaac aggaatcaag aacttaaaaa
    12541 tcggatttaa ccgcgttttc ggttattata tagaaattac aaaagcaaat cttggaaatg
    12601 cagaactgga gaagtatgaa cgaaaacaaa ctttagcaaa tgcagagcgt ttcatcacac
    12661 cagaactaaa agaattggag acacaaatcc tcgaagcaga agaaaaatct gttgatttag
    12721 aatatcagtt gtttttagct gtgagagaag aagtcaaaaa ggctatccag ccgctacaag
    12781 tacttgcaaa agcaatcagt tcagtagatg tgctacaaag ttttgcaacg atcagcgaac
    12841 gctatcagta tgttcggcca gaacttgtca gtgacaagca tcagcttctg atcaaagacg
    12901 gacgacatcc tgtagtagaa aaagtattag gacatcagga atatatccct aacagcgtag
    12961 aaatggcaga agacgaaatg atcttgctga ttacaggacc gaatatgtct ggtaaaagta
    13021 cgtatatgcg acagttggca ttaactgttc tgatggcgca aatgggctgt ttcgttccgg
    13081 caaaacaagc agttttacct atttttgatc ggatttttac ccgaattggt gcaagtgacg
    13141 acttaattgc tggacaaagt acttttatgg tagagatgat ggaagccaat caagccctgc
    13201 gttatgctac gccaaacagt ttgattttgt ttgacgaact agggcgagga acagcaacct
    13261 atgacggaat ggcgttggcc caagcaatca tcgaatatat ccatcgtcat gtacaagcaa
    13321 aaacactctt ttctactcat tatcatgaac tgacggtact tgagaaagaa cttccgcagc
    13381 ttaaaaatgt ccatgttgga gccgttgaaa aagacggaga agttgtcttc ttgcataaaa
    13441 tgatggatgg acctgcggat aaaagctacg gcatacatgt ggcaaagatc gcaggattgc
    13501 cttccaatct actagaacga gcagcagata tcctgcatac gctagaaaat ggcgataacg
    13561 gacaccatac tgttccagct gaagtagaag agaaaactaa aacagccaaa caggagcaaa
    13621 taaaaccacc tgcagaaaaa gtaaatcaag accatataca agaccgtgta aaagaagaaa
    13681 accaacagct ttctttattc agtgaattat ccgaaaatga aacagaagtc attgaatcac
    13741 tgaagcaaat aaatttacta gaaatgacgc caatggatgc actaaacaca ttatatgaat
    13801 tacaaaaaca actgtaggaa gaaggcggat atcgatggcc aaaatccaag aattatctga
    13861 acgtttagct aatcagatcg ctgcgggaga agtagtggag cgaccagctt ccgttgtgaa
    13921 agaattagtg gaaaatgcaa ttgacgccgg cagtactcag attgatatct tagttgaaga
    13981 agcgggactg cggacgatcc aagtgattga taacggagaa ggcattttgg cagacgacgt
    14041 agagaatgcc ttcaagcggc atgcgacaag taagatccat aatcgcgacg atctttttcg
    14101 catacgaacg ctgggctttc gaggggaagc tttgccaagt attgcctctg tttctgaaat
    14161 gactctggaa acggcgaatc aggaagaaaa acaaggcacc tatctgtcgc tgaaaggcgg
    14221 agaagttatt gagcatcgac cagcaccact gcgtcaagga acgaagatta ccgtcagcaa
    14281 tctgtttttc aatacgccag cccgcttgaa atacgtgaaa acactgcaca cagaattagc
    14341 gaacatcggg gacatcgtca atcgactggc attgagtcat ccatcgattg cttttcgttt
    14401 agtacatgat ggaaacaaaa tgctggcaac tgctggaaat ggcgatctga aacaaacaat
    14461 tgcgggaatc tacggattat ctaccgcaaa aaaaatgctg aaaatcgaag gaaaagatct
    14521 agattttgag ttatttggct atgtttcatt gcctgaggtg actcgtgcca gtaggaatta
    14581 cttatcgacg atcatcaatg gacgttttat caagaacttt tccttgaata aagcaatcgt
    14641 tgcaggttat gggtcaaaat taatggtcgg tcgtttccct attgctgtat tggaaatcaa
    14701 aatggacccg cttttagtag atgtaaatgt tcatccgacg aagcaggaag tccggctgtc
    14761 gaaggaaaaa gaactaacag agctaattag taacgccatt caaaaagcat tgtctcaaga
    14821 aaatctgatt ccaagtgcag cggataatct acggtttaag aaaaaagtac ctgatcagcc
    14881 taaaacagaa caaatcgaaa tcgatttgtc ctacgctttt gaagaagaaa aacctaaaaa
    14941 gccaagtagt ttgaattttg atcgagagac aggtaccttt ttcgtagaag aactaaagaa
    15001 cacatttgtt gacagaattg ttgataagtc tgtggaaaat gttgaaaact attctggttt
    15061 ttctgaagaa aacaccactt atccagaaaa aacagcgaaa acggtggata agtttggtga
    15121 aaatgttgat aactttgtag aaaaaacagt ggaaaaatct gaaaagaaag aatttcccat
    15181 tgtggataac atggagaaaa caaatgaaga acaagcagat aaatccgtag aagaggatga
    15241 aaagatttac gaggaagaat tgacactcca tcctgaattt gatttcactc aaggaaacga
    15301 caaaacatta gaacaagcca tcaagaaatt agaaacggaa aaaccagtac aacgttttcc
    15361 tcagttggaa tattttggac agatgcacgg aacgtatctt tttgcccaaa gtagcgatgg
    15421 gttgtacatc attgatcagc atgctgccca agagcgcatt aagtatgaat atttccggga
    15481 aaaaattggg gaagtatctg atgatctgca agaattgctt gttccatttg tgttagacta
    15541 tccaaacagt gatgctttga aattaaaaga gcaaaaacac ttgctagaag atgcagggat
    15601 ttatttagaa gaatttggtc aaaacagctt tattgtacgg gcccatccta cttggtatcc
    15661 caatggagaa gaagaatcga ttattcggga gatgatcgat atgctgctga caactggaag
    15721 tgtgagtgta aaaaaattcc gtgaagcaac agcaatcatg atgagttgca aacgatcgat
    15781 taaagccaac cattatttga atgaagcaca agcacgtgtg ctgctgaagg atctggcacg
    15841 atgcgaaaat cctttcaatt gtccccatgg ccgtccagta ttgattcact ttaccaattc
    15901 ggatatggaa aggatgttca aacgaatcca agacccgcat taagtcaaag agaagaggga
    15961 aaacatgaag attgtattag catcccaatc accaagaaga aaagagttat tggctcatat
    16021 cgtttcagaa ttcgagattg aacctgctga tatcgatgaa acgccatttc cagacgaaca
    16081 gcccgtcgat tatgttcgta gaatggctga agaaaaagca cgaactgtct gggaacaaga
    16141 tgaaaaagaa gaccatttag tgattgccag tgacacgact gttgttctca atcaagagat
    16201 catgggtaaa ccagaagatc tggctgaggc tgaattcatg ctgaaaaaac taagcgggga
    16261 aacacatact gtatatacag ctgttgtttt aaaaacaaag gaacgagaag aacggatact
    16321 agcagaagca catgtcactt tttatccttt gacagacgaa gagatcaaac gctacttgga
    16381 gacgggggat tatgcggata aggcaggggc atacggcatc caaaatgctg cgagtgtatt
    16441 cgtcaaagaa atttctggag attattacag tatcgtagga tttccgattg gtgctgtcaa
    16501 tcaggcactg aaaacattca tttaaacaaa aaagcggact atctgatttt cgattgaaaa
    16561 tccatgatag cccgtttttt ttattgaaaa gattacttat ctactctgac taagatttta
    16621 cttcagcttt accgactgct tcgcccgggc gaacaagtcc tgattggtca agagaaagtc
    16681 ctgcaacttt ttcattattg gtcaaagcaa caataatgtc agttttcttg ccagcttgtt
    16741 ctatttctgg caaatccatt tcagcaatca gggtctctgg tgtgacaagg tatccttctt
    16801 tcacgaatac attgaaagga acacccttta gttcaacagt atctaatccc atgtgaacaa
    16861 gtacttccag cccctcatta gaaagaatac caatggcatg ttttgtttca aagacactcg
    16921 tcactttacc gtttactggt gcatagactt tgccgtttga cggttcgaca gcataaccgt
    16981 cacccatcat tttttgtgag aaaactgggt catttacttc agttatttcc ttgatggtcc
    17041 ctgcagcgac tgcgaataat gtttcttttg ttgcaactgc cgtaccagtt gctgaaacag
    17101 gttcgaaaga ctgtggcgtt gctgttgcgc catttgctgt ttgaagttgt acatcagcag
    17161 cattttcagg aacagcagga tcaatcttat tcaaaattcc ctgacgtcta aagatgatcg
    17221 tcaagccaaa tggaatgatg acagcaatga tcattgcgat gatgaaaggt acccatgttt
    17281 ctgcttggat agctagtatt cctggcaaac cgcctactcc gatcgcgtta gctcgaacac
    17341 ccatcaagtt agcaaacatg ccagctaatc cagagccgac cattgcagcg acgaaaggat
    17401 agacgtattt caaattgatt ccgaacatag caggttcagt tacgcctaag taacaagaaa
    17461 tcattgctgg aatcgagatt tgttcttctt ttttattccc acgatgtaag aagatgatgg
    17521 ctaagactgc agagccttgg gcgatatttg ataaagcaat catcggccat aaattcgttg
    17581 atccgaaatc tgcaatcagc tgcatatcga tggcattggt catatggtgg agaccagtaa
    17641 tcactaaagg agcatacagg aatccgaata cggcactaaa caaccagctg atagaagacg
    17701 tcaatccagt atttacgata tttgagatcc agcttccgac tgtccagccg ataggaccta
    17761 aaataacgtg tgcagctaaa acagttggta acaatgaaaa caatggaaca aaaatcatcg
    17821 agatcgactg agggatatat ttgcggaaga agatttctag atacgctaac ataaaccctg
    17881 ccagcatggc tggaataact tgcgcttgat aaccaatcat ctggacttga gcaaaaccaa
    17941 aatcccaaaa tggaatatct gctgcggctg tggaagctac actataagca tttaataatt
    18001 gcggagaaac taacgtaatt cctaaaacga ttcctaatat ttgtgtagta cccatctttt
    18061 ttgcaatact ccaagtgata cctactggaa gaaaatggaa aattgcttca cccggtaacc
    18121 acaaaaaggc attgacacca ttccaaaact tcgagtgttc aacgattgtt ccaccaagac
    18181 tttcaaactg gataccttca agtacatttc tgaatccaag gatcaatcca ccgacaatga
    18241 ttgcaggaat cagtggtgta aagatttctg ctaaaacggc aatagcacgt tgaacgggat
    18301 gaaggttttg ttttgcagct gcttttcctt gttctttgga aaccccttca actccagata
    18361 ctgcaacgaa gtcgttataa aaagtagaaa catcgtttcc tatgatgact tggaactgtc
    18421 ctgcattcgt gaacatccct tttacgctag gtatatcttc gatcgctttt tcgtccgctt
    18481 ttcctggatc attcaggaca aaacgcatac gagtcgcaca atgactaact gcagcaatat
    18541 tttcttttcc gccgatatca tgtaacagct tctcggcatc tgcttgatat tttgccatat
    18601 caataaaccc ctttctctta acttgtctat gcaagttgtt tcttcattta ctatactttt
    18661 tttcggagag aattgcaagc gttttattaa aaagtacgag agaaaatgat atcgttttaa
    18721 aaagcttaaa ataagctttt cttaaaaata ggaacattga aatacaaaaa gagctgtagt
    18781 atagtgaaga aatgatggaa gaacgagtga gggaaaaaac atgaataagt tcaatgaaat
    18841 ttttttagat ttggaacaga aaattttagc gaaagaatat cctccgcatt cgcttttacc
    18901 cagtgagaac caattgatca aaatttatgg tgtttcacga gaaacaatta gaaaagcgct
    18961 gaatcttttg actacggaag gttatattca aaaaaaacaa ggaaaaggtt cgatcgtact
    19021 ggatcggaat cgcttcgact ttcctatttc tggattgact agctataaag agctacagga
    19081 gacccaacgt attccaagtg aaacgatcat ccatacgtta gaagaaacag aagtgacaaa
    19141 agcattgaac gagatcactg gctgggagat cggtgcgcct gtttggcatt tgatacgcga
    19201 aagaaaaatc aatggcgaag tagttatatt agacaccgat tatctattaa aggaaatcgt
    19261 tcctatttta actccagcac aagcacaagg atcgatctat gaatattttg aaaatgagct
    19321 ttctttgacg attgcttacg cgcaaaagga gatcacagta gaagaagtaa cggaaatcat
    19381 gaaaacagcg atggatcttc acgatgacac ttatgcagta gtagtcagaa gcctcgttca
    19441 tctggaagat acccgctgtt tcgagtattc tgagtcggtt caccgattag ataaattccg
    19501 tttcgttgag tttgcccgac gccgaaaagt gtaaatagtt caaaagaaag caaattgttg
    19561 gcatctgtcc actttggcgc tatgatagga tcacaaaggg ggcattttta atggataaac
    19621 caataagacc agaaaaagaa gaactgaaag aaaagttatc acccatggct tatgcagtca
    19681 cacaagaaaa tgcaacagaa caaccattta gcgggaaata tgatgatttt tacgaaaagg
    19741 ggatctatgt agatatcgtc agcggtgaac cgttattcag ctctgcagaa aaatatgatg
    19801 caggttgtgg ctggccatca ttcagcaaac caataacgaa aagacaagtg cgcgaaaaag
    19861 ccgatttttc tcatggcatg catcgaatag aagttcgaag caaagaagca gattctcatc
    19921 tcggacatgt gtttaccgat ggaccagtag atcaaggtgg tctacgttac tgcatcaatt
    19981 ctgcctcact gaaattcatt ccatatgaaa agatggatga attaggttat ggggaattca
    20041 aatcacttgt agaataatgg aaatacacaa agagcggtac aatacgggga tttactcgat
    20101 cgtccgctct ttttttattc aagcgattgc tatttgaaag aaacgctccc tagactcctt
    20161 ttttcacaag gtgaaatgtg ctacaataaa gggaacgtat gtgtgcaaaa gaaagaggac
    20221 tagaatatgt atgaatatct gacaggtgtg gttaccttta tcaaccccta ttacatagta
    20281 gtagaaacca atggtatcgg ctaccagatc gcactaggaa atccttaccg ctacagtagc
    20341 aaactaaatg aaaaaataaa aatttatgtc catcaggtaa tacgtgaaga tgcgcatctt
    20401 ctgtatggat ttgattcgct agaagaaaag caactgtttt tgcgattagt aagtgtttca
    20461 ggaatcggtc cgaaaagtgc ccttgcaatc atggcaagcg aagaccactc cggtttgatt
    20521 caagcagtag aatccagtga tgtcacttat ttgacgaaat ttccaggagt agggaaaaag
    20581 acagcacaac aaatgatctt ggatctaaaa ggaaaattcg gtgagctgag tatcgataca
    20641 ccttttagtc tatttgatga agcaaacaca aaagacgcta cagctctatc tgaagcaatg
    20701 gaagctctct cggctcttgg ttacagtgat cgagaaatca aacgtgtaga aaaacaatta
    20761 aaagaagtag ataatcagac aacggatgag tatctacgcc aagcactcaa gctaatgatg
    20821 aaaaaataag gaggagacca aatgacagat gaatacttgt tatcaccaga atctggcgaa
    20881 aatgaactga gtttggaaaa atctcttcga ccacagctgc tttctcaata tatcggacag
    20941 agaaaagtca aacaagaact tcagatttac atcgaagctg ccagaaatcg ggaagaacct
    21001 ttagatcatg ttctgttgta cgggcctcct ggattaggaa aaacgacgat ggccatggtc
    21061 attgctaatg agatgaccgt aaatattcgt acgacgagtg gccctgctat cgaacgtccg
    21121 ggtgacttag tagcaatctt gaatgaactg gaagcagggg atgtcctgtt tatcgatgag
    21181 atccatcgat tacctagagt ggtagaagag atgctttatt cagctatgga agatttctat
    21241 gtagatatca tggtaggaca agggccaacc gcacatccgg ttcatttccc tcttcctccc
    21301 tttactttga ttggcgcgac gacacgggca ggtatgcttt ctgcaccttt acgagatcga
    21361 tttggcatca tttctcatat ggaatattat gacgaacagg atctgcagga gatcgtttta
    21421 cgttcttcgg atatttttca aacagagatc attgaagaag gggcattgga aattgctcgc
    21481 cgatcacgag gaacgcctag aatcgctaat cgcttgctca aacgggttcg tgattttgcg
    21541 caagtccaag gagacgggaa aatcgatcgt cctatagcgg atcaggcact tactttgcta
    21601 caggtcgatc aggctggatt ggattatgtt gaccagaaat tgttgcgaac aatgatcgag
    21661 ttatatggcg gcggaccagt agggttaagt acgctttcag taaacatagg agaagagcgt
    21721 gagacggtag aagatatgta tgaaccgtat ctgatccaaa aaggattttt aaagcgtaca
    21781 cctagagggc gaattgctac tgcttatgcc tatgaacatt ttggttacga ctatcatata
    21841 tgatttttgc ttttagattg gaggaaaatg aatgagtgat acaacacgta cgaaacaatt
    21901 gatcaaaaac gtattgattg atttgtgcca aacgcaatct tttcgaaaaa tcagcgtcca
    21961 aaaaatcgca aatgaagcaa aaatcaatcg acaaacattt tattatcatt ttacagataa
    22021 atacgatttg cttcgttggg tctattgtga ggatgcattg cattatttag atccacaatg
    22081 tctttcactt gataattggg aagagcaagc attgaaaatg ctgaaagcaa ttgctgagaa
    22141 gaaagaattt tatgcaaata ctgtgctttc tgacccagaa attttacaaa aagagttttc
    22201 tcaactgatc caaaaatcat ttatcaagat ttttgaacaa atggatgaag aaagacaact
    22261 ttctgcaaga gacaaactat tttatgctcg ttttttttct tacggttgca gtggtgtatt
    22321 agtcaattgg atcacgaatg aatatgaaga aacaccgatg gaaatcgcta tgcagctttt
    22381 ccgtttagcc aaggatacgg aattatattc ttatcgtctt tatgcaaaag aagagttgga
    22441 agaacagtaa tagaaaaatt ttttgttgga aaagcaataa tcgctaaaag gagatgggga
    22501 aatgacaaaa attttatttg tttgtttggg aaatatttgc cgttcaccga tggcagaagg
    22561 cttgctgaga aaacgaatag caatagaagg aagggaagca gaattttttg tggattctgc
    22621 tgctacaagc acgtatgaag ttggaaagac accgcaccct ggaacgaaaa aaatattaaa
    22681 ccaagaaaaa gtagatatga cgaatatggt tgctcggcaa atcacacccc acgattttga
    22741 aacttttgat tggatcattg gcatggatca agaaaatgta gaagaactga aacgaagagc
    22801 accacgctca gcgcagggga aaatccattt gtttttaagt agcattcctg gaaaagaaaa
    22861 agaaaatgtt cccgacccat actataccaa taactttgat gagacatatc acttgatcac
    22921 agaaggcctg aatcattggt ggaatatttg gattgacgaa aataaaataa caaacaattt
    22981 gtaaagttga cgctttcttt ttaaggcgat gttagagcat ttccttgttt ttttgtaaaa
    23041 tgttaggtaa aataaaatta attaagtaac actttcttag ctaaaggagg agtgcattcg
    23101 atgaacgaag agacagtatc gccaatgaag aagcttacga agttagttac tcatacttcg
    23161 tatcaacttt ttgattcttt tgtaaatgac actgaagaac atgctaagaa tgtacgattg
    23221 caaggaaaat ttattcttga gctgcaaaaa gctgctactg aacatagtct agttatctta
    23281 caagtaagag cgaatcgttt gagtagcaag tatgaaacga tctcaggctg ggtggcgaca
    23341 aaaacggtcg aaaaagaccg agtgatgatc cgcctacaaa acgaagagca gcagatccgg
    23401 atggttcctg tagaacaaat tgaaaaaata acaatccttt caccagcagg ggaacaggaa
    23461 cgattatcaa aataatttat accagataga aaagcacgta tgagcttgta ccatcaatca
    23521 ttactaatca aaaataaccg aatgctttaa gctgatatca gtaaaacgga acgttttagg
    23581 atgagtatca cagcatgaca gcattgggtt gattagttga atcgattgga tggtacaagt
    23641 ttttttgata aaaaaaggtc ctgctagccg gggagctagc aggataagga gttaaaaatg
    23701 aaaaagtgtt gttagggttg tttgttggta tgcttatata atagtcacta gttgtgaata
    23761 ttttgtgacg aaagttttcg aaagttgtta agtttttttg cgaaaactat ttttatatca
    23821 tatatatgtc taaaatctat attataaaag aaatgttgtt tgatcagctt ctcttcggta
    23881 ttagttcaaa acaaaacggc aaagtatatg gagctacttt gccgttttgt ttctctaata
    23941 ctcaggtcag tttatagacg ctcaataaac ggtgttcaaa agccagtttc tcggtattaa
    24001 gtcaaattta tgcaaaaatg tgagaagcca ttttcaaaaa atatttctta atactcgaaa
    24061 ctgagcggct ttttcacgac ctctcaataa acagtgttca agagacagcc tatcggcaat
    24121 aaggctgaac gtctctttca caacctcttt tatcaatctg ctacttcttc tttcttagcg
    24181 aaactccaaa tcagtccgat aacaaaaccg ataactgctg gtacgatcca ccccatcccc
    24241 aatttgaaaa acggcaagta gctttccgca acatataaaa gattttgaac aataggtgtt
    24301 tgtttgatag tttctgggca agcgttcaac ccatcaaaaa tcgaagcaat caatgtaaaa
    24361 taagtagtca ttcgataaac agccgtacgc tgcttaaaca aaggaccaac taacactagt
    24421 aaaatcagtg tcattgctaa tggatagatg aacatcaaaa caggcaacga aaattgaatg
    24481 atattcgtca agcctacgtt tgccgctaaa caaggaagga tactagcaag tgcaacaaag
    24541 aatgcgtaat ttctttgcgg aaacaaatct gtgaatgttt ctgcaaaagc tgtaatcaaa
    24601 ccaataccgg tcttcaagca agctaatata acgataaatg ctaataagat actgccataa
    24661 gtacctaaat aatgatcagc aatctgagcc agcgtgatcc cgccattttc acttagagca
    24721 aagctaccta agctcatagc acctaaataa gcgagcaaag tataaatgat tcccatcaat
    24781 ataatgctga tcgcaccaga tttgatcgtg tctttagcga tttcagcagg ttttgttaca
    24841 cccatgtttt ggattgttgt gacaatgatg atcccaaatg ctaaagcagc aagcgcatct
    24901 aatgtgttat atccttgagt aaaccctgtg aaaaaagcat gctcttgata atttggctga
    24961 ataggcgctt gatttacaga acctagtgga ttcaaaaaag caaggagaag caaagcacct
    25021 aataaaacca aaaatgcagg attcaggaac tttccaacat aatctaatat ctttgtcggt
    25081 tttcttgaaa accaccaagc cagcacaaaa aatacaatac tgaaaattgc tagatatagc
    25141 gtctgtctgc taggatcgat aaatggtgct aaaccaatct cgaaagatgt ggttgcaagg
    25201 cgaggtaaag caaaaaacgg tccgataact aagtaaagca agatggtaaa aaccatcgca
    25261 tatgtttgat ttatccgttt tgctaattca tatacacctg agcttttcga tacaccaatg
    25321 gcaatcaccc ctaagaaggg aagaccgata ccggtaacca agaaacctag attcgcccaa
    25381 aaggtagcag cgcctgcttc ttgacccaaa tgaacgggaa agataagatt tccagcacca
    25441 aaaaatagac cgaaaagcat ggagcctata aaaagatagt ctttaaatga taattttttt
    25501 gtcatacata actcctttac ggttttttta aaagtaacaa aatttcgaaa aatatgcaac
    25561 aattttcaga aaatttccta tataaaacga cggttattta ataacgtaag tgataaaata
    25621 aaagcgctga atcatctgag gaggaatcat cgtgacaaag tcgagattag aagcatttac
    25681 tgatggagtg gtagccattg ttttgagagt tcttgtatta gagatcaaga tccctaatac
    25741 atctagcttt cattctttat gggtcatccg aaatacatta ttagcttata ccatcagctt
    25801 tatatttgtt gcggtgatct gggtgaacca tcatcgtata tttcagatgg cagaacgaat
    25861 taattaccga gtggtctggt cgaatatctt tttgctcttt tggctgactt tatgtccttc
    25921 tgttactagt tgggtcgggc gtaaccctga agctttttgg cctgagatgg cttatgtctt
    25981 agtatacacg atgtggagtt tttcttttgg tatcttgatg aggcaaatca tgaaagcaaa
    26041 taaaccagat tcccatgtcg tgagagtttt gtcaaaggac cgtcgaagtc ttatctccat
    26101 gatgattaac ttatgcttga ttgccggtgt cttttttgta ccgttgatcg ggttattcgg
    26161 acgttttttt gtatctggta tttggatttt ttcctataaa aaagcagatg cctattatca
    26221 gcggttgttt ccggggaaat agaaaagagg ctgggacaga agcttttaac tccaagaaat
    26281 aagccgaaat ttcacaaaaa tttaagaagc aattttcgta aattccttct tatttctcga
    26341 agctgaccac ttctgtcaca actactttct tacttcttac gttcatattt catatgatag
    26401 attagtaata gaagcaagaa ccacacaggt gttagtaata aagcgattct cgtatctttt
    26461 cctaggaaca agacgacaca cacaaaggcc atgtaaagaa ataccagata attactaaat
    26521 gggtacaacg gcattttaaa tgaatgagct tttttgccga ggatactttt acgatatttc
    26581 atgtgcgtgt agacaagcat actccagata aacaagaaac aagtagtggc aatactgctt
    26641 actagtgtga atacttcact aggcatcacg taattcaaga taacagaaat gaaaatgacg
    26701 atagtagaaa atgtcaaagc attccctggc acatgatgtg ttgttaactt tttgaatttt
    26761 ttaggggcac tgccttcttg cgctaaagaa cgaagcatac gtcctgtact atatattgca
    26821 ctgttgcagg cagaagctgc tgcacttaat acgactaaat tgatcaagga agcagcaatc
    26881 gtgatcccaa tttccgaaaa tacttctaca aatggactgt tatctggatt caagctgttc
    26941 catggataga ttgccataat gacaaaaaga gatcctaagt aaaataagat gatcctgata
    27001 ggaatattat tgatagcttc cggcaacact ttttcaggat tttctgtttc tccagcgact
    27061 aatccaacta attctatccc agcaaaagca aaggttacca tttggaaagc taagataaat
    27121 ccttttgcac cagttggaaa gaatccgcca tgagaccata gattcgtgat actggctgtg
    27181 ccaatatctg tcgtatagtg ggtgaggatc atataagctc ctacgaggat aaaggcaatg
    27241 atcgctacaa ctttgatcaa tgcaaaccaa aactctaact caccaaacaa tgagacagcc
    27301 accatattca agcaaagaag aagacctaat gcaatcagtt caggaagcca ctgaggaaca
    27361 ccaggcgccc agtagcgtac atacattccg atggctgtta aatcggccat tgcaatcgat
    27421 acccagcaga accaatacgt ccagccagtg atgaaagccg cttttttgcc aagatattct
    27481 gcaacaaaat ccagaaaaga atggttattg gtgtttgata acagaagttc gcctaacgca
    27541 cgcataatga aaaaacagat gcatccagta ataagatagg cgagcaatat cgaagggcca
    27601 gctagctgga tagactttcc ggaacctaaa aacaaccccg ttccaatcgc tccgccaata
    27661 gaaatcagtt ggacatgtct atttttcagt ccacgctgca gttcttgctc ttcattcatt
    27721 tttaaatcat cctaactaaa aaagtttaat tacttccata ttaaaagagt tttctgaaaa
    27781 acacaactaa gattccaaaa ttttcagaaa tttacctttt atgagtaatt cataaaaaag
    27841 aactgcggta aacaccgcag ctctttgtcc caatcagcta ttgatcctaa gcatttaaaa
    27901 tacgtgaaag gaattctttt gtacgttctt ctttcggatg gacaaagata tcttctggtg
    27961 taccttcttc tgcaatcaca cctttatcca taaaaatgac acggtcagat acatcttttg
    28021 caaattccat ctcatgcgtc acgataagca ttgtcaagcc agtatgtgct aagtctttga
    28081 tcgtattaag cacttcgcca accatttcag ggtctaacgc cgaagtaggt tcgtcgaata
    28141 gcaatacatc tggattcatt gataatgccc gagcaattgc gacacgctgt ttctgtccac
    28201 cagataattg tgctggtttg gcttcgatat aacgatccat accaactttt tccagatttt
    28261 cgattgctac ttttttggca gtttctttat ctcgtttcag gacagttgtt tgaccagaca
    28321 tacagttttc caagacattc atattgttga acaggttgaa ggattggaaa accataccta
    28381 gattcgttcg atatttaggt aaattatatt taggagctaa tacattttct ccattataaa
    28441 tgatctcgcc gcctgttggt ttttccagta aattgataca gcgcaagaaa gtagatttac
    28501 cagaaccgga agaaccgatg atcgtcacaa cttcaccttt attgactgtt acgctgatat
    28561 ctttcaatac ttcattttca ccgaagcttt ttcttagatg tttgatttca ataatttttt
    28621 cattcatgat tcttcctcct tattcctgca aatttgaatt gtcgatttct tcaagcttgg
    28681 tataagctga aggaccatcg attttctttt ctaccgcccg caggattctg gtgatcgtaa
    28741 aggtcatgac taagtagatg atcccgacga tcgtaaatgt ttggaagaat tggaaattcg
    28801 caccggaagc agagtttcct tggaagaaca aatcagctac gccgattaca cttaaaactg
    28861 ctgtatcttt gatattgata acaaactcat ttcctgttgc tggcaagata ttccgtaaaa
    28921 cttgtggaat aacaactttt ctcatcgttt ggccatgggt cataccaatg gcttgcgcag
    28981 cttcgaactg cccttcatct actgcaaaaa tccccccacg aacgatctct gtcatgtaag
    29041 ctccagtatt gagagagaca ataaacaaag cggcaatcgt tcggttcaat gaaatgccaa
    29101 aagcgagtgc cagaccgtag aagatgacca ttgcttgtac catcatcggt gttccgcgga
    29161 atacttcgac atagatagaa agaagtgcat ttccgatttt ttggaagaag cgagtcagtg
    29221 catttcctga ttcaggtacc gaacggaaga caccaatcag taatccaagt gtagtaccga
    29281 caactgtccc gatcaatgcg atgaacaagg taagtacagc acctcgtaag aacatattgc
    29341 cgtattgttt ccaaatcgta gtgaaatcag aaatgatgcc aggtttttct tcactatcag
    29401 ttgcagcagg ctgatctttg actgcttggt ccataatttt ggttcgttca tcgttagaga
    29461 tgccagaaag aatctggttt actttttgga tatccgggtc gcctttacgc ataccaacag
    29521 aaatctgaac atcttccggg tttgtctgga agccatctgc accgctgaat tcaagcattt
    29581 ccaagtcttt gttcacacta gttgccgtta caccttccgg acgttcgctg acataaccat
    29641 cgatcattcc agaagctaaa gctgttctca ttgctgagaa attatccatt gcttgttgtt
    29701 tctgcacatc tggtatctga tcgatcacag tatagtggaa tgtatttaac tgagcagtaa
    29761 tctttgctcc agaaagatct gctaaacttt ttgcttttgc atatttgcta tctttttgaa
    29821 ctactactac taattgtgat tcgtaataag gatcggtaaa atcaatttct tttcgtcgtt
    29881 cagcagtagg gctcattccg gcgatgatcg cgtcgatctt gcctgattga agggcaggag
    29941 ccagaccatc ccatttcgtt tggacgatga ctaatttttt ccctaaacca tctgctactt
    30001 ttttagcgat ttgaacatca taaccgccgg cgtaactatt ttggccttga attggcaagg
    30061 cattattctc atctgtttgt tgagaccagt tgaagggtgc atacccagcc tccataccta
    30121 cgcgaaactc atcgctcggt gttttttcat ctgcatttgc cagagatccc acactgaata
    30181 gtgagaagac aaaagcaaat agtacggcaa ctgaaaccat attttttttc atttttttct
    30241 ccctttctga ttatatgata cacgagcggt ctagatgaaa tgcggtaaag ctttatctga
    30301 aaaaataaaa agcgaccgat ttatagatat aaaaaggccg ctaaaattcc ataaggtgat
    30361 aaacctctca caaaacatag cgcaacttag catcactgct aagacagtcc gcaagctctt
    30421 aacttgcgtc ccaacataca ctccaagcaa aaaagtgcac atttcggcga tcctccctat
    30481 tcctgcttct ccatgtttgc ttcactccac agggttaatg attctcgcga cctctacctc
    30541 attgattgag gcatctcgta ttcagttatt tatacaaaaa aagtttacga tactcgagag
    30601 gctttgtcaa tctataactg ctgttttttg aaaaaatact gataaatata gaaatcttta
    30661 cttttaccga aaaaacaatc aaaatgtgaa tcatctatcc attgcaaacg atagtataat
    30721 tatacacaaa aatgtatgta atacaataaa aaaagaacta gaacagaaaa tttcctgtgt
    30781 ccagttccat ctcgatcatt taagctgccg ctttctttgg ctcacgtcct aggatcgctt
    30841 gcaaaagaac aacgacgcca taaatggcta atattagata aatcaaactt gagtattggg
    30901 tgattcccca gccccatgtt cgatatacgg ctaatgtaat ggctaaaaca attgcggcaa
    30961 tcacgttgaa taaagcaaaa gcccaaagat ttccattttc tttgcgtaca agataaaaaa
    31021 cagagaaata aacgcttaac gctaaccagg cgatacaagc cagaataacg ccccaataaa
    31081 taaagaatgg taacatatcg attcacctca cttttataca gcagtattgt aacacgtttt
    31141 cattcttttg tcagtgaaca ttgtgataaa ttgttcaaat gcccataaca cttttttatt
    31201 cccatttttc tgacttttca acaataaatt tgttcgagta tgggctataa tggaaagtga
    31261 ggtgacaaaa catgaaacaa ttaacagtag acgaagcagc aaaagagata ttagcttatc
    31321 agcaaattac atttttgacc ggtgcagggg tatccacacc ttctggagtg ccagactatc
    31381 gatctttgtc aggaatctac cacgggatgg agcagccgga atacttgctg agtcatcaag
    31441 caatggtgaa tgaaccggat aaattctatc aatttgtcaa aaagatttat catcctgaag
    31501 caaaacccaa tatcattcat ttggaaatgg cacatctagc gaaagaaaaa aatgtttggg
    31561 ttgtttccca aaacatcgac ggtttgcacg caaaggctaa aagcccgcaa ctcgtcaatt
    31621 ttcatggaag tttgtatcaa tgtcattgtc gaaaatgtca acaaacggta gactggaaag
    31681 aatatcttca cagcgacaaa catcaagcat gcggaggaca aatacgccca gacatcgtgt
    31741 tatatggtga agggtttcaa gatggagtca tggagcaggc ggcttatgcc gtcagccaag
    31801 cagagctgat cgtcattgtt ggaacgagtt ttcaagttca tccgttttgt gatctggtac
    31861 agtttcgcca gccgtcagcc aaaatcttag taatcaatca aacgcctgtt tatttagcgg
    31921 agccttatac gtttattcaa gaagacggag cggttgtttt caaaaaaata caagaattag
    31981 gagcagatta tgactgaatt caaaaccgaa aaagaaaaaa tgattgcagg cgagctttat
    32041 tttgccaatg atcctgagct ggttgccgac cgtatgtttg caagaagtca aagccaaatc
    32101 atcaaccaag cagaatcagc ggaactgcga tcccaattgt tgaaagagac ttttggccgt
    32161 acagggaaaa aaatctatat ggagccagtt atcaattttg attacggata taatatattt
    32221 gttggggaga atttttatgc aaatttcaat tgtacatttt tagatgtcag cacgattgaa
    32281 atcggtgata actgcatgtt cgctccgaat gtccagcttt acacggcgac gcatccacta
    32341 catccagtga aacgtaacag cggtctggag tacgcaaaac ccattaaaat cggtaataat
    32401 gtttggttag gcggaggagt tatcgtcacg ccgggtgtca ctctagggga caacgtagta
    32461 gtcggcgcag gttcggttgt gaccaaatct tttcccgaca atgtagtgat tgcaggaaat
    32521 cctgctcgta tcatcaagac agtagaggaa gaactaacag aagagtctct tgaaacatta
    32581 cgtcataaga tcgatatgat cgatcgagag ttgattgctt tattagaaaa aagaatggat
    32641 acggtaacta aaataggaca agtgaaaaaa gaagaaaacc aagcagttta tgatgcaaaa
    32701 agagagcagc aagtattgga taaagttgtt agtctgctga aaaataaggg atataaggaa
    32761 acaataacag atacgtatgt tgatttgatg aaacactcgc gcgaatacca aaacaaaatg
    32821 aaggaggaat aagtgtgatc gaaggagaga cacggcgaaa acaaatcatg caaaccttgg
    32881 caaatacaga aaaaccagtg agtgctagca agtttgcgaa aacttttgga gtcagccgtc
    32941 agatcattgt aggcgatatt gctttactac gagctgcagg taaaagtatc gtggcaacag
    33001 caagaggtta tatcctcgaa acagaagaaa ccaatactgg gcacatcagt aaaatcgctg
    33061 tccagcatgg aaaagatcaa acagaagaag aactgagact aatcgttgaa aatggtggtg
    33121 aaatcattga tgtaattgtg gaacatcccc tttacggtga gttagcagga actttgcaca
    33181 tcaaaacgcc acaagatatc gattccttta tgagacgtta tcaaaaaagt aatgctactt
    33241 tgttatccga attaacaggc ggcatccatc tgcatacgat ccgctaccca gaaaagcata
    33301 tgctgaaaaa catcaaacaa aaactagctg aagcaggaat cctctacgaa ggataacatc
    33361 gtagctgcta ctttttatcc tagaaactat tctatcatct ctgagataaa cacccatatg
    33421 gaagaaataa gaaaaaataa gaaaagatgc gtatccaata cacagaattt tttggaattg
    33481 cgatttctag atgtctatct aaatttcgat tagtggagaa acataaaaaa gggaagtgca
    33541 gcttttgagc actccccttt tttataaagc tttcttgtag catagtatcg aaggaacctg
    33601 ccaattctct actcgatcac caatacgcct tcttttagtg caaatggatt ttgatcgttg
    33661 atgtggtcat agaacatcac accgttgata tggtcgattt catgttgaac gacgatcgat
    33721 tcataattct tcaaacgaat cttgtgtttt tcaccattca tatcataata agacacagtg
    33781 attttggcat ggcgaacaac atatcctggt acttctcgat caacagataa acagccttca
    33841 ccttccccta aacaagcatc ttgaacagaa tggctcaaaa ttttaggatt atacataaca
    33901 gtacttagag aaggtccatc tgcctctggg tcaggacttg gtacgtgaac agcaatgatt
    33961 cttttagaaa tatctaattg aggagcagct aagccaacac cgcctcgtaa gtgtagttct
    34021 tcagctttga ttggatcttg gctgtttttt aagaactcaa gcatctcttt tcctaaagag
    34081 atatcttctt cgcttaatgg aagagaaact tcttttgcaa cttcgcgtaa ggtcggattt
    34141 ccttcacgaa tgatatcatc cattgtaatc atggaatatg tacccctttc ttaaaagact
    34201 gtgtttcaag tctattttcg cattatagtt taccacaaaa atggaaaagt agtagtaaga
    34261 ttgtttgaaa actacctttt cttaatgatt aactacttga ttttcaaccc ggtttcaggt
    34321 aaagtatagc tgtgtgctct aggcacatga cctgccactt ggttaagcga atcaccaacg
    34381 ttttactcag tcgcagggga ataatcgaaa gaggtgaaag aaatggcaat ttcaaaagaa
    34441 cgcaagaatg aaatcatcaa cgaatacgct cgccatgaag gggatactgg ttctccagaa
    34501 gtacaaatcg ctgtattaac tgaagaaatc aaccacttga atgaacatgc acgcgtgcac
    34561 aaaaaagacc atcattcata ccgcggccta atgaaaaaag ttggtcaccg ccgtaacttg
    34621 ttagcttact tacgtaaaac tgacgttcaa cgttaccgcg aattgatcaa acgtttaggc
    34681 ttgcgtcgtt aattttagtc aaaaagagtg ggattcacat gaatctcact ttttttactc
    34741 gtatatcaat taagtaaaat tccgtgatca agctagatgg actcctacta accaaatgaa
    34801 agctgctcaa aagacggctt tcggcagttt tcatttgttt agtagcgtct tgctttcacg
    34861 agaaggaaaa ggagaagtta tgtcagaaaa acaagtattc aaaacgacat ggggcggccg
    34921 tcccttacaa atcgaagtcg gtcagctagc aaaacaagca aacggagccg ttttagtccg
    34981 ttatggagat acagtagtct taagtgcagc tgtggcctct aaagaagcaa aagatacaga
    35041 ctttttccca ttaacgatca attatgaaga aaaaatgtat gcagcaggga aaatccctgg
    35101 aggtttcatc aaacgagaag gccgtccaag tacggaagca acattgactg cccgtttgat
    35161 cgaccgtcca attcgcccga tgtttgcaga aggcttccgt aatgaagtcc aagtaacgaa
    35221 catcgtgatg agtgtagaaa cagactgctc gccagctatg gcagcaatgc taggttcttc
    35281 tttagcacta tcaatctcag atatcccatt tgacggccca atcgcaggcg tagaagttgg
    35341 tcgtgtaaat ggcgagtacg tcttgaatcc aacagtagaa caagcagaac aaacagatat
    35401 cgaattgact gttgccggaa cgaaacaagc catcaatatg gttgaaagcg gcgcgaaaga
    35461 agtttcagaa gaagacatgc ttggtgcatt gctgtttggt tttgacgcta tcaaagagtt
    35521 agttgctttc caagaagaaa tcgttcaagc agtagggaaa gaaaaaatgg aagtgacttt
    35581 actgcaagtt gatgaagtat tgaaaaaaga aatctttgat gcttcttacg ctacaatgaa
    35641 agcagcagta atgactgaag aaaaacttgc tcgcgaagat aatatcgagc aggtgaagat
    35701 cgatatccgc gaagcttatg ctgagaaatt tgcaggtcat gaagatgaag atcacttgtt
    35761 gaaagaagtt aaacaaatca cagaagatct tgaaaaagat gtcgtacgtg aattgatcac
    35821 catcgataag atccgtcctg acgggcgtaa attggatgaa atccgtccat tgtcatcaga
    35881 ggtcagctta ttgccacgtg tacatggatc aggtttgttc acacgaggac aaacacaagc
    35941 tttatcagct tgtacactcg cacctctagg agagcaccaa atcattgatg gtttaggggt
    36001 ggaagtcagc aaacgattca tccaccacta caattttcct caattttctg ttggttctac
    36061 aggacgtgca ggctctccag ggcgtcggga aatcggacat ggcgcattag gtgaacgtgc
    36121 gttagcacaa gtaatccctt cagaagaaga atttccttat acgatccgtt tagtagcaga
    36181 agtattggaa tcaaatggtt cttcttccca agcaagtatc tgtgctggca cattagcatt
    36241 gatggatgca ggtgtaccaa tcaaagcgcc agtcgccggt attgcgatgg gacttgtttc
    36301 tgatggtgaa aactatacga tcctaactga tatccaaggg ctggaagatc atcttggcga
    36361 tatggacttt aaagttgccg gtacaaaaga tggtatcact gctttacaga tggatatcaa
    36421 gattcaagga atcacagaac aaatcctaac tgaagcatta actcaagcaa aacaagcacg
    36481 tatggaaatc ttagaagaat tgacttcaac gattgctgct ccacgtgaag aattaagcca
    36541 atatgcacca aaaatcgaaa tgatccagat cgaacctgct aaaatcaaag atgttatcgg
    36601 taaaggcggc gatacgatca acggaatcat cgatgaaact ggtgtgaaaa tcgatatcga
    36661 tcaagatgga aaagtaagta ttgcttcagc tgatgcagaa atgatcaaaa aagctatcaa
    36721 gatcatcgaa gatttgacca aagaagtcaa agttggtgaa gtttatctag ggaaagttgt
    36781 ccgtatcgaa aaattcggtg cattcgtcaa cttgatcaaa ggaaaagatg gacttgtcca
    36841 tatttcccaa ttagcaaacg atcgtgtcaa taaagtcgaa gatgttgtta aactaggtga
    36901 tgagatcctt gtgaaagtaa ctgaaatcga taaacaagga cgcgtaaatc tgtcaagaaa
    36961 agcaatgttg aacgaagatg gttcaacgaa aaatgaagaa aaataattta attcatagga
    37021 ataatgtact tgtgatcgtg aaggtgacag ggggacaata attattcgaa aaaaggggac
    37081 tgtgacaaaa gccgtgtcac agtccttttt tttcgaataa acggtgttca aaagcttgca
    37141 tctgcggtaa taagtccagc caagttaaaa acattaggag ctgtttttat ttggctgttc
    37201 ttattacttg atgcagaaca gcttttttac aacctctcca ataaacgatg ttcaatcagc
    37261 tgtccctcga tattagctca aaaaacggca aaatatgagg aactattttt ccgtttgttt
    37321 ctctaatact caggtcaaac cgctgatttc acaacctctg attcacggtg ttccagaagc
    37381 agcttctacg gaaataagct gaacactttt gttacaactt ctttttttct ggagtttctt
    37441 ggcgcatatt tttggagtcc ttctgaataa agcgtaaaat aaaagtgaga aatatggata
    37501 aaggagggaa aggagatgct gaatttttta tggtcgttat ttatcggcgg tatattaggt
    37561 ttgatagctg gcgcaatttt aggaaaggaa gtcccgggag gcgtatctgg aaatatcatt
    37621 atcggttttt taggtagttg gctcggcgaa tttttactag gaccactggg accggaactc
    37681 ggcggttttt atttgatacc agcattattg ggtgcaattt tttgccttgc tctttattca
    37741 tttgtggcaa gacgtatcag agaacgtcca aagaaagaat aattttattc aaataagttg
    37801 acacttcagt gtgttacttg gataattaga agcaatttag gaaagcgggt gtcatggata
    37861 agaatatcat ctatcattca agcttaccta aaggatatcc atataacaag aaatgaggga
    37921 aaaacgtggc aacaacgcac aataccaaaa agatatcaat ggctggcctg cttgtagcga
    37981 tgggagtcgt ctacggagac atcgggacaa gtccgcttta cgttatgaaa tcgattgttg
    38041 aagataatgg cggattgtca ggaatcaatc ctgattttgt tatcggatca gtctcattga
    38101 ttttttggac attgactcta ctgacaacaa tcaaatatgt cgtgattgca ttgaatgcag
    38161 ataaccatgg tgaaggtgga attttctcac tctacacact tgtccgaaaa ggtgggaaat
    38221 atttgatcat tcctgcaatg atcggaggag ctgccttact agcagatggg gtattgaccc
    38281 ctgctgtgac ggtaacgact gcgattgaag gcttgcgagg aattccgcag ttttttgacc
    38341 ggttcggtaa taaccagaac gtgatcgtca ttatcacttt agttattatt tttgttttat
    38401 tcatggtcca acgctttggt acagatattg tcggaaaagc attcggacca atcatgttcg
    38461 cttggtttac ttttttaggc gtaatgggat taatgaactt cagtcaagac tggacagtta
    38521 tccgtgcact gaatccttat tatgctatcc agctgctttt tagtgcagat aacaaattag
    38581 gcttgttcat tcttggaaat attttcctgg ctacgacagg tgcagaggcg ctttattccg
    38641 atcttggaca tgtcggcaaa cgaaatattc gtctgagctg gccttacgtc aaaatttgtt
    38701 tggttatcaa ttattttggg caggctgcgt ggttgttgaa tgtttatcaa aaccctgaag
    38761 cacagcaaat caagaacctg aatccattct tccaaatgat gcctcaaagt tggatcgtga
    38821 tcggcgttgc ttttgcaaca gtcgctgcag tcattgcttc gcaagcattg attacaggtt
    38881 cgtttacgct agtttcagaa gcaatcaagt tgaaactgct gcctcggtta aagatcatgt
    38941 atcctgggaa cagtatcggg caaatgtata tcccagcagt caatctgatt ttatggatcg
    39001 cctgttctct agtggttgtc acattccgaa cgtctcatca tatggaagct gcttacggtc
    39061 tatcgatcac tgtgacgatg ttgatgacaa cagcactgct ttacttttat cttatccaaa
    39121 atgggcattc tcgttggctt gcatatttag ttaccttttt cttcggtgcg atagaagtcg
    39181 ttttctttat ctcaagtgtt gtcaaattct tccgaggcgg ttttgtagcc gttctgattg
    39241 gtttgatcat cttagtcgtg atgtttattt gggaacaagg gaacattatt cgcgaatcgt
    39301 caaccgagga tgtgtctttg agagattata tcccgcagct tagagaatta cgtgatgata
    39361 cttctttacc aatctatcag acaaatgtgg tcttcttggt tcctgatatg gctgaagata
    39421 aagttggccg acagttcatc tattcgattt tagataaacg gcctaaaaga gcacgagtct
    39481 attggtttgt tcatgtggaa gtgacggatg agccatatac gaaagaatat caagtagata
    39541 tgatggacac tgattttatt gtccaagtga atctgttctt aggtttcagg gtccaacagg
    39601 aaatcaacgt atatgttcgc caagtcatcc atgatttgat gaaacaaggc cgtttgccaa
    39661 aacagccaca aagatactct ctgacaccag ggcgagaagt tggcgatttc caatttgtcc
    39721 tgattgatga agtagtttcc aatgtgacaa ctctaggaaa atggcaacgt caaatcatgc
    39781 aggcaaaact ggcaatcaaa aaaatcgcta cctcgccaga aacatggttt gggctagagt
    39841 acagcgaagt aaaacatgaa actgttccgc ttatcattgg aaatactcgg aaaacatggt
    39901 tgaaagagcg gttgtgaaag tagcgctttg acctgaacta atgtcgaatg tcagctactt
    39961 gaacaccgtt tatcagaggt catgaaaaag ctgtactgca acaagcagta agaacgagca
    40021 gataaaaaca gcttctcatg tttttcatct gtttgggctt aatgccgtag atgcaagctt
    40081 ttgaataccg tttttatcag aggtcatgaa aaagctgttt agcaacaagt aacgcaatga
    40141 ttttgaagtg ataaggagat gtggctgaaa aatgccgcat ctcctttttt attcctaaat
    40201 atgaatacgc attattttca atctttttgt agaaaaaggg tacaatagaa aaagagaaag
    40261 gcgggagaat atggttatca atttcattat tttatttttg atgattgcaa ttacttcatt
    40321 ttttgtagca agtgaatttg ctttagtgaa aatcagacga tcaagattgg aacaactaga
    40381 aaaggaaaat gtaaagaacg ccaagctagc tcttcatgtt acacaccatt tagataacta
    40441 tttatcagct agtcaattag gaattacgct aaccggtttg atcatcgggt gggtcggtga
    40501 aggatctgtc gcggctttac tagaaccagt gatcggtaaa ctacctttta gttcggctat
    40561 tagcagtaca atctctgtgg cgctgggatt tattttagtt acgtatatcg atgtggttat
    40621 tggcgaactg cttcctaaga gctatagtat cgtcaatacg gagaaggtcg tcttgttcgt
    40681 agttaaacca cttcattatt tttataaagt aatgtttcct tttatttggg tattgaatca
    40741 ttcagctgca gggctaggga agctattagg tgtgcgtctc gtatctgaag gagaagagac
    40801 attgtctcaa gaagagctga cattagtcgc attgaattct tatgaaaaag gagagttgac
    40861 caaagaagaa tattattatt tggaaaacgt gtttgaattt gatgatacct tggcaaaaga
    40921 tatacaagta gaccgtacgt ccatgcaagt attcgaagct tcagaaaccg tcaaagaagc
    40981 gataaaagct tcgattgaac aaggacatac tcgctatccg gttatcttag aatcaaaaga
    41041 caatgtttta ggttatgtca ccttgccaga tttgatcaag caatcttata aagatgacca
    41101 gctgacagta gaacagttga ttgaagagcc gatcgtaaca actgaaacga tccctataaa
    41161 aaaattattg acgatcatgc gaaaaaaggg taagcatatt gctatcttaa aagatgaata
    41221 cggagggaca agcggcttag tgacgattga agatatttta gaagaaatcg tcggggaaat
    41281 ccgagatgag acagatttgg acgaagcatt gatagcggaa cagtcagatg gctcttatat
    41341 catctccggc aaactaacat tagatgattt ccagcgttat tttcatgtag agattccaga
    41401 gtttgaagaa acgaatttta ctacacttgc gggctttgct tctagccgat ataaagaaat
    41461 aaaagcagga acaatcattg agattgcatc attccgtttc acggtattag aataccagca
    41521 tgcacatatc gattatttca aagtagagtc cacggaaaga aaaacagaat aaaagaaaaa
    41581 aggctgtgac attttttgtc acagcctttt tgttatctaa aaaacctgtt ttcggtaaac
    41641 gaagaagata ttttcaataa aagttttcat ttttagccgc aacgatttct tttaatcaag
    41701 gctatcttgc ttctgcatgt tctaatgtga aatgttcctc ttcacccatg tgtttcaatt
    41761 gtttcatcga acgcatataa gagtaaagca acacggcaac agaaccgatc caagctaaag
    41821 gagaagctgc tgctgctcct gggtatcccc acattcctgt aagaacgata gctgcaaaag
    41881 aacgcataag caattccatc ataccggcga tcgtagggat cttgctttgt ccaagccctt
    41941 gaagtgtgta gcgtaaaatg aacaggactg ctaagatcca gtaaagagac ccattgatat
    42001 tgaagtaggt ttgtgctaat tcaaatacac gtgtttctga tgggctaaca aacaattgaa
    42061 caaagaaatg accgaagaaa atgacgatag cacctgctaa gagactaaat ccgacactca
    42121 tcagtaaagt ttgacgtacc ccttgcaaga ttcggccata ttctttcgca ccatagtttt
    42181 gtgcagtgaa agttgccatt gcaataccaa atgacatcat cggctgattt gcaaattggt
    42241 cgatacgacc agcagcggct tgagcagcta cgacatctgt ccctagacta tttagtgctg
    42301 cttgtaacac gatagcacca acagcgatga tcgaagattg aaacgccatt ggcaaagcag
    42361 catttaggtg tacagcaaac tcttcttttt caaatttgaa atcttttttg cgtaactgta
    42421 acaaaggcat ttttttcttg atatacacta gacacagtac gctggaagaa acttgtgcga
    42481 ttactgtagc aatagctgca ccttccacac ccatgccaaa atagataata aagatcaaat
    42541 caagtacgac gttgatgatg accgcaatga tcagaaagaa tagtggtgtg cggctgtcac
    42601 caagtgcgcg aatgacatta gaaagcaaat tgaaactgac tgatgcaaag atccctaata
    42661 aaataattga aataaatgtt tgtgcttgat caagtatttc aggaggagtt tgcatcaatt
    42721 gaagcatcgg acgaataaac aggagactta ggacagttaa aatcacagtg acgacaagac
    42781 taatgacaac actagcagca aaacttttct tcagaccccg ataatctttt gcaccgtaac
    42841 gttgtgcggt aataatggct aaaccagctg ttaaaccttg ggcaaagcca atgatcaaaa
    42901 atgtcaggct tccagttgca ccaaccgctg ccagcgcatt tttacctagc gtctgaccca
    42961 cgatgatcat atcgatcata ttgtagaact gctgaaaaac atttccgatc agtaaaggaa
    43021 cagtgaacat caaaatcagt ttagcggggc ttccttttgt taaatcgcgc atcttttaaa
    43081 ctccttatat ctttaaaatt tagttagtag tatatagcgt ttatcctgag aataccaatt
    43141 agaaatcaat gataatttat gaaactccat aaaaagaagc caagaattga gtcaaagaat
    43201 caacaacttg gcctggtctg taaaataagg gacatccacg tttatctatc accttaatat
    43261 tgtctaatct cacttggtaa aaggatcgtt tctctcgtac cgtcatcata ggcgtatact
    43321 tgagcaggaa attggttcga ataggcaaac ggcaaatcaa tatccatttc cacattgctt
    43381 cctgcttgag aaaaatgcat cgtcactttt ccacgatttt cgatcaaatc aaaggttaat
    43441 atattttcta atggaatgac tcccttaaga tctaggtcaa taataaacca tatactgtca
    43501 atcaattcat ctggcaaact tgatacgaca ccaactgaag cgtaacggtt gcggctgcta
    43561 tcaaaacttt caaacatctg tttaacctcc ttcgaatcaa cgaatgtttt atttttataa
    43621 ttaatcctag gataaactag aaatacttac ttcaacgagg gttcattcta ataaagaatc
    43681 aggaaaaatg caacaataat tgaatacttt ttttcaaagt ataaattttt ataaaggtac
    43741 gataaaagta tgcgaggaaa agcttggtaa attaggattt ttcgtttgta aagccattta
    43801 caaaagtgga agccccatat gaaaataaac gattagaaat aaaaaaggcg tgttaaaagc
    43861 tgctgacccg ccgcttctaa caccacctga ttgtttcagt attattcttc tgttgaaccg
    43921 atcacttctg gttcgccacc ttcacctgtg ctttcttctt cttcactttg agcgactaca
    43981 gcagcgattt gctcttcact gtcggtcacg aatttgtaat cgtcgttttt tggaagatcg
    44041 gctactgtaa tagaatcacc taattccaag tttgtcacgt caacttcaat tcgttcaggc
    44101 aatttgtctg gtgtagcaga tacgactact gtatacaagt tttgagctaa cactccacca
    44161 gatttagcac ccactgattc gccaacaaga acgacttctg tttcgacttc tgtttcttcc
    44221 gacatgttta cagatagaaa ttctgcatgt tcgaaatgat tggtaaaagt gttcgtttgg
    44281 atcttgtgaa ccaatgtatt gactttttgt ccatcgatgt ccatcgtgat gactgtgttc
    44341 gctccatgtt cacgcaacaa tttgcttagt gtcaaaccat cgaatgagat aggggtactt
    44401 tctactttat acccataaac aacagctgga actttccctt cgtgacgaag tttgtttctt
    44461 aatgaacggg gacgtacttc tcttttactt acttctaatg atactgacat aaccaaaatt
    44521 cctccttaaa atgtgactgt ctgctttggt aacggacaga ttttatttaa gtgaattcta
    44581 aaggctaatc tcgtgcacat catcgaaggc agggcgctta aaaaagcgca aaaggagaga
    44641 tgcgcgcatc tccccttaga aagtcaggat tcaacttttc tccaccaggt cattacgctg
    44701 tgtatgagaa atccaattca ctttactatt taaccgtaac gtgaactgtt aattaagtca
    44761 aggaataact atgatgatgt atttgcaagc aagaatattc aaggtataat taattagtag
    44821 taataagaaa ggaaacagca tgcgtttaga taaattaata gaagaaaaat tagaaacgac
    44881 acgcaaggaa atgaagcggc tgtttgcttt gaagcaagtg atgatagatg gcacagtcga
    44941 atttcgacaa aatcgaaatg tagatagttt actgcatcaa atcgaagtag caggtgaaca
    45001 cttagaaaca aatgagtcct attatcttct caataaacct gaaaaagctg ttacagctgt
    45061 atcagatcca gaaaagacga cagtaatcga tctgattgca agtgccgatc gtttccaacc
    45121 attatatcca gtgggaagac tagatagaga tacaacagga ctattgctga tcacttcgaa
    45181 tggacaattg ggatatgaaa tgctacttcc agaaaaaaag gtttcaaaga cttatcaagt
    45241 agttgtgaac gaaagggtga caccggcaga cgtaacagca tttgaaaatg gaatcgtatt
    45301 cgctgatggg tatcaatgta agccggctga tttaaaaata ctcaaagcag acaagcacca
    45361 aagtacggtt ttattgacga tccaagaagg aaaattccat caagtcaaaa agatgttctt
    45421 agcccaagga aagaaagtga ccgcattgaa acggctaagc atgggaccat tggaattaga
    45481 tgaacagcta ggagtaggcg agtatcgatc gttgacatta gcagagatga aaaagttaag
    45541 tatttatttt aaataggagg tcaaaaagat gaaatataaa actttattgt ttgatgtaga
    45601 tgatacattg ttagattttc aattaactga agccaatgct ttaagtgctt tgtttgcaga
    45661 acaaggaatc acattgacac cagaaatcga aaccagctat aaggctttga atcatcattt
    45721 atggcaggag tttgaaaaag gcagactatc acgagaagaa ataataggca gtcgttttgg
    45781 gttacttttc cagcaatttg ggaaaacagt agatagtgat caaatggaaa aacgctaccg
    45841 ccactacttg aaccaaggtc atgatcttgt tccaggaagt ctagaaatcc tgaaaaaggt
    45901 ccatccgcat gctgatcttt atattgtgac gaacggtgtg tcgcacacgc aacatcggcg
    45961 attaacagat gcaggaatgc ttgattactt caaggatatc tttgtctcag aagctgtcgg
    46021 agcgcaaaag ccgatgaaag aattttttga tcatgtcttt tcccacattc ctaattttga
    46081 taaaaagcaa actgtgatca ttggtgattc tttgacttct gatatcaaag gaggaaatat
    46141 ggcagggatc gatacgattt ggttcaatag aaataaatta ccagaaatcg aagaaatcca
    46201 gcctacttat cgaatcgatt cattagagga actttattca ttattggaaa ttgaaaagga
    46261 ttaattttca gaaaaatata gcattttcgt gttttttcgc tataatagat tagaatgaaa
    46321 aaacggatac gaaagaggtt ttagtcaatg acaaaaagca agccaattat cattggtgtg
    46381 acgggtggat ccggaagcgg aaagacaagt gtcagccgtg cgattttcaa caacttcccg
    46441 gaccattcca tcatgatgct tgaacaagat tcttattaca aggatcagag ccatttgagt
    46501 tttgaagaac gattgaacac aaattacgat cacccatttg cctttgataa tgatctgctg
    46561 attcagcatg taggcgactt actgaactat aaagccattg aaaaacccgt gtatgactat
    46621 gtagcgcata caagaagtca ggcaacgatc attcaggaac caaaagaagt gatcattcta
    46681 gaaggaatcc tgattttaga agacgaacgt ctgcgtgact tgatggatat caaagtatac
    46741 gtagatacag atgatgacat tcgtatcatt cgtcgtatca aacgggatat ggaagaacgg
    46801 ggacgcacat tggactccgt gatcgaacaa tatttgacag ttgttaaacc gatgtatcat
    46861 caatttatcg aaccaacgaa gcgctatgcg gatatcattg ttccagaagg cggagaaaac
    46921 cacgttgcta ttgatttgat tacaacaaaa gtagctagtt tcttaaacca taaatagtgc
    46981 gcagcgaaac agttgaacgg actgcgaccg tttgaaaagc aaggattctg ctttttgaac
    47041 agtcaccagc caactgtttt tttatttata acaagaggtt gtgaaaacag catcttgacc
    47101 taagtattaa aaagaagaaa cggaaaatag ctgtttatat tttaccgttt tttgagctaa
    47161 taccgaaggt cagctgattg agcaccgtga agtagaggtt gtaaaaaagc tgtctttgca
    47221 tcaagcgata agagcatcca aataaaacag ctcctcacgt ttttcgttgg gttgggctta
    47281 ttatcgtaga tgcaagcttt tgaacaccgt ttaacgagag gttgtaacag aagtggtcag
    47341 atgcaataaa taagaagaaa cttacgaaaa ttatttctcg aatttgtgtg agatttcgac
    47401 ttattttttt gaagaagctc ttttgtcgca ccccc
//
LOCUS       DAPBCS010000018        47232 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPBCS010000018 DAPBCS010000000
VERSION     DAPBCS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.018"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            44..1003
                     /locus_tag="RD521_001207"
     CDS             44..1003
                     /locus_tag="RD521_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7770955.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1173..1853
                     /locus_tag="RD521_001208"
     CDS             1173..1853
                     /locus_tag="RD521_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDT7770956.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1943..2977
                     /locus_tag="RD521_001209"
     CDS             1943..2977
                     /locus_tag="RD521_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDT7770957.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            2991..3326
                     /locus_tag="RD521_001210"
     CDS             2991..3326
                     /locus_tag="RD521_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770958.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3310..5790
                     /locus_tag="RD521_001211"
     CDS             3310..5790
                     /locus_tag="RD521_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770959.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5846..7405
                     /locus_tag="RD521_001212"
     CDS             5846..7405
                     /locus_tag="RD521_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7770960.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7402..8766
                     /locus_tag="RD521_001213"
     CDS             7402..8766
                     /locus_tag="RD521_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7770961.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8937..10007
                     /locus_tag="RD521_001214"
     CDS             8937..10007
                     /locus_tag="RD521_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770962.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10022..10690
                     /locus_tag="RD521_001215"
     CDS             10022..10690
                     /locus_tag="RD521_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770963.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10710..11288
                     /locus_tag="RD521_001216"
     CDS             10710..11288
                     /locus_tag="RD521_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7770964.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11689..12600
                     /locus_tag="RD521_001217"
     CDS             11689..12600
                     /locus_tag="RD521_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7770965.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12978..13895)
                     /locus_tag="RD521_001218"
     CDS             complement(12978..13895)
                     /locus_tag="RD521_001218"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDT7770966.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14097..14534
                     /gene="zapA"
                     /locus_tag="RD521_001219"
     CDS             14097..14534
                     /gene="zapA"
                     /locus_tag="RD521_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDT7770967.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            14592..15140
                     /locus_tag="RD521_001220"
     CDS             14592..15140
                     /locus_tag="RD521_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDT7770968.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15255..17615
                     /locus_tag="RD521_001221"
     CDS             15255..17615
                     /locus_tag="RD521_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDT7770969.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17702..18016
                     /gene="trxA"
                     /locus_tag="RD521_001222"
     CDS             17702..18016
                     /gene="trxA"
                     /locus_tag="RD521_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDT7770970.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18198..19976
                     /gene="uvrC"
                     /locus_tag="RD521_001223"
     CDS             18198..19976
                     /gene="uvrC"
                     /locus_tag="RD521_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDT7770971.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20121..21020
                     /locus_tag="RD521_001224"
     CDS             20121..21020
                     /locus_tag="RD521_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7770972.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21013..22275
                     /locus_tag="RD521_001225"
     CDS             21013..22275
                     /locus_tag="RD521_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7770973.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22436..24334
                     /locus_tag="RD521_001226"
     CDS             22436..24334
                     /locus_tag="RD521_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDT7770974.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(24664..25383)
                     /locus_tag="RD521_001227"
     CDS             complement(24664..25383)
                     /locus_tag="RD521_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDT7770975.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            25720..26052
                     /locus_tag="RD521_001228"
     CDS             25720..26052
                     /locus_tag="RD521_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7770976.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26039..28042
                     /locus_tag="RD521_001229"
     CDS             26039..28042
                     /locus_tag="RD521_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDT7770977.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28061..28531
                     /locus_tag="RD521_001230"
     CDS             28061..28531
                     /locus_tag="RD521_001230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDT7770978.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28552..29136
                     /locus_tag="RD521_001231"
     CDS             28552..29136
                     /locus_tag="RD521_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDT7770979.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29151..30137
                     /locus_tag="RD521_001232"
     CDS             29151..30137
                     /locus_tag="RD521_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDT7770980.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30127..30438
                     /locus_tag="RD521_001233"
     CDS             30127..30438
                     /locus_tag="RD521_001233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDT7770981.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30460..32241
                     /locus_tag="RD521_001234"
     CDS             30460..32241
                     /locus_tag="RD521_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDT7770982.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32234..33610
                     /locus_tag="RD521_001235"
     CDS             32234..33610
                     /locus_tag="RD521_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDT7770983.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            33617..34252
                     /locus_tag="RD521_001236"
     CDS             33617..34252
                     /locus_tag="RD521_001236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDT7770984.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34282..35637
                     /locus_tag="RD521_001237"
     CDS             34282..35637
                     /locus_tag="RD521_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDT7770985.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35829..37223)
                     /locus_tag="RD521_001238"
     CDS             complement(35829..37223)
                     /locus_tag="RD521_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDT7770986.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37315..38286
                     /locus_tag="RD521_001239"
     CDS             37315..38286
                     /locus_tag="RD521_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDT7770987.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38283..39065
                     /locus_tag="RD521_001240"
     CDS             38283..39065
                     /locus_tag="RD521_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDT7770988.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39234..39605)
                     /locus_tag="RD521_001241"
     CDS             complement(39234..39605)
                     /locus_tag="RD521_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7770989.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39739..40182
                     /locus_tag="RD521_001242"
     CDS             39739..40182
                     /locus_tag="RD521_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDT7770990.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40225..41034)
                     /gene="yidA"
                     /locus_tag="RD521_001243"
     CDS             complement(40225..41034)
                     /gene="yidA"
                     /locus_tag="RD521_001243"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDT7770991.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41053..42420)
                     /locus_tag="RD521_001244"
     CDS             complement(41053..42420)
                     /locus_tag="RD521_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7770992.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42404..43234)
                     /locus_tag="RD521_001245"
     CDS             complement(42404..43234)
                     /locus_tag="RD521_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDT7770993.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43397..43834
                     /locus_tag="RD521_001246"
     CDS             43397..43834
                     /locus_tag="RD521_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDT7770994.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43910..44530
                     /locus_tag="RD521_001247"
     CDS             43910..44530
                     /locus_tag="RD521_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDT7770995.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            44719..45225
                     /locus_tag="RD521_001248"
     CDS             44719..45225
                     /locus_tag="RD521_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDT7770996.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45242..46141
                     /locus_tag="RD521_001249"
     CDS             45242..46141
                     /locus_tag="RD521_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDT7770997.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46228..47112
                     /locus_tag="RD521_001250"
     CDS             46228..47112
                     /locus_tag="RD521_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDT7770998.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        15602 a         8159 c         9957 g        13514 t
ORIGIN      
        1 ctcaaaatag ttccgacgaa agaattataa ggagtgagta caaatgacct ataaccatct
       61 tacaccaact gaactcgtaa tgatagaagc ttatttcaat caatcacaac ctgtttcaaa
      121 agtagccaat ctgttacaac gttctcgaca aactatttat aaggtctatc gttttcttaa
      181 atctggtgga agcgcaatga gctactataa acaatataag aaaaacaaac gtaactgcgg
      241 aagacatccg attgttttac ctgatgagca acaggaatac attcaaaaaa aggttgttca
      301 agggtgggct cctgatgtcc ttattggtcg agcagagttt cctattgctt gttcggtacg
      361 cacactgtat cgaatgttta aagataaaac ctttgacact cacgatttac caatgaaggg
      421 aaaaagaaaa ccaaacggtc ataaagaaaa aagaggaaaa caagggtttc gccggtcaat
      481 ccgagacaga aagacagact atacccagtt tgatcatgaa tttggtcacc tggaaggtga
      541 cactatcgtg ggtggaaaac ataaaagcgc ggtcatcaca ttagttgaac ggctatctaa
      601 agtcatcatt accttgaaac ccgaaggcag acaggctaaa gacattgaaa ataggctgaa
      661 tcaatggttt cgttgtgtcc caaaaaactt atttaaatcc atcacatttg attgtggaaa
      721 agaattctca aattggaaaa agattagtaa taccaacgat atttcaattt attttgcgga
      781 tccaggaaca ccttctcaac gaggattgaa tgaacattca aatggcttat tacgtaaaga
      841 tggtttgtat aaagctatgg attttaatgg tgtatctgaa acctttatcc aatcggtggc
      901 atcgaaacga aatcatattc ctagaaaatc attacactat cggacaccaa tggaagtatt
      961 tttgagttac gtcacaaaag atgatttgtc taacttaatt tgacaaacga aacttaataa
     1021 aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat acaaaaaggg
     1081 tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt ctctactata
     1141 cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt gggacattat
     1201 tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag ggtcaagcga
     1261 cttcaaaagg agatattacc tttactgaac caacaaatac cgtggagcct cttaacccta
     1321 ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact ggccagacag
     1381 gtcctttaac actggatgta gttcccgaat tgccatttgg tacacacgag attgaaagcg
     1441 gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc caagtatcag
     1501 atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca gtttcggttt
     1561 cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt ggaacatcca
     1621 ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact gtaactgggt
     1681 tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa ggtctgggaa
     1741 catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa gcagcagctg
     1801 gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca tagagtatga
     1861 aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta ccgcagttgt
     1921 tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt cgtttttttc
     1981 tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt cagcaggaga
     2041 ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg gttattttga
     2101 tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca attcttcaga
     2161 tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg gtggaacgat
     2221 cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg tccgtgacgt
     2281 tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa ctgtacctat
     2341 agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta ttcatgtttc
     2401 acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca aaaatcgtat
     2461 tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac cggatctgaa
     2521 acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc ttcgtttcca
     2581 aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct tttatgaaaa
     2641 tcagctttat attgaaaata cgtctaatgc atttttagtt gcaccaaatt cgaattttca
     2701 cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg cagaaatcat
     2761 agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga tcaaaaaaga
     2821 aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat ctttcccatg
     2881 gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc tgtttttgtg
     2941 ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg ttgaaaacat
     3001 cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa gagccagtcc
     3061 aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca attccgcatc
     3121 caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt tataaagaat
     3181 ctaatcaaca tggcgtattc ccggagacga atgaacaaaa gatccgtttt ctttatttga
     3241 tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga aaggaaaagg
     3301 aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt ttttacttcg
     3361 ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg agcaacgcct
     3421 cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa tggtggacgt
     3481 agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt gcctaaattg
     3541 actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac ggtccgtttt
     3601 cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat cactgcacaa
     3661 ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag tgatgtatta
     3721 aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat caaggttact
     3781 agcggaagtt tattatcagc aggaagccat accttgcgtt ttgggatggg gtcgtgggat
     3841 acaaatactc tattgacaga tattattgct attattacgc gaggcgaatt caacgtggac
     3901 gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga aaacggagat
     3961 aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc ttctatgtct
     4021 aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca agatcgtatg
     4081 ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac tggcggaaac
     4141 ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg gaatagttac
     4201 atctcaccaa gtgattcaac aggaaaatat aatacatttg ctgataccac agaatacgtg
     4261 gatggaaata accagaacct tccaggctct accaatagtc ggacattgac gatggggctg
     4321 actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt caactatttt
     4381 acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag cacaagtgga
     4441 ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg attgctttct
     4501 cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt accaacaaac
     4561 ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca agtcacggcc
     4621 aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta ttcgaaagga
     4681 acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca atcaaataac
     4741 gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca atattatttt
     4801 gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc gtacaaatgg
     4861 cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa ttttccatct
     4921 gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg agatcccctg
     4981 tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac aaaatctggg
     5041 gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac ttatatcaat
     5101 ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca aggagtttct
     5161 gtcccagcag atctattgag caaaatagga gataccattc atcttactta caactatcaa
     5221 ttaaatactg tagatacttc tttacaatcc gtcagcatac tgacaaaggc agctgtactt
     5281 tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt ccaaacatct
     5341 gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc agatgatttt
     5401 acatttggta acgatctccc caaaccgttg aagacaagtt attatgaatc aaaaggcgat
     5461 ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca actgacaggt
     5521 acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac aaaattatac
     5581 ttcaatcatt ctggatcaaa gcaattgatt cagcaaggtc aaaacacgtt gatttatgag
     5641 agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga tggtttactc
     5701 ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg catggtcaca
     5761 tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga tctgtgataa
     5821 ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa aatcagaaat
     5881 gcttgaagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac aacggcaaat
     5941 caaacttttc caacttttat tagaggaaaa aggagaggtt tccgttaata acgtacttac
     6001 ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac aagctaactt
     6061 ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat taatcagaca
     6121 tggaaatata gcagcagtcg atgtttatta tcattgttac ttgcgctatt ctacaaaata
     6181 tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac ttgtcatgga
     6241 acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg attctttaaa
     6301 tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc aaattcgtta
     6361 tttttatttc caattatttt ggtttggacg tccctatctt gaaaacaaaa aagaatacag
     6421 tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt ttgctattac
     6481 agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc gaatgcgctg
     6541 tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc aaacgacaca
     6601 atttttattg gacctccata catttttgac ccgttacttc tttcaatatg cctttccttg
     6661 gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta cattagatac
     6721 acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg acgtagtagc
     6781 tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt atgtttttcc
     6841 agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc attttgccta
     6901 ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt taactgaagt
     6961 agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat ttgctattgt
     7021 tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc gctatgcatc
     7081 tattttacgg acgctgatca agcagttaga attacgtctg caaattgctt atgatcttcc
     7141 ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat tgctgaatac
     7201 gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca ttttgactag
     7261 ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca ctagcaacga
     7321 atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt tagaaaaatt
     7381 gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca tgaaggcatc
     7441 acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact gatcgatgaa
     7501 gatcataaag atggactagg ctatgtttca aatggaatca acctttattt aaaaggaaag
     7561 atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag tggggcaaaa
     7621 ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct catcctcgca
     7681 tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc gacaggttcc
     7741 tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta taaaaatcta
     7801 gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt gatcttcggt
     7861 gctggttaca ttggattgga attagcagat gctttgagaa ataaaggata cattattcat
     7921 ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat gatcaacagt
     7981 tttcaaaatc agctgcaaac taagcaaata aatttttatc caaatgaatt cttgatcgat
     8041 tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tgtctcaaac aataaaggca
     8101 gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa agaaaaagtg
     8161 gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag tgatccggac
     8221 atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg gcatctcttt
     8281 ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac tttgagtggt
     8341 cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat agactacttt
     8401 ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa taccggttcg
     8461 tgttcaggag aatttgatct atttccacaa tacgacgaag aaaacaaaac agtaaatgca
     8521 aagctgatct accatccgga tacattagaa ataattggcg gacagctgat aagccaagaa
     8581 ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac tacgattcca
     8641 cagctagcag ttgaggattt tggatttctg tctgaataca cctctcgttt tcactacctc
     8701 aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt agataaaaga
     8761 ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta atcaatcgag
     8821 aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata ttcgccaaaa
     8881 atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg agttaaatgt
     8941 ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt gcaggggtga
     9001 tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc ttagctcaag
     9061 ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg aaagatgcaa
     9121 attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc gaaggtggga
     9181 aagtggcgta tcttgcccaa acacctggag tcgtgactag taaagacagt acagaagaag
     9241 gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg cccaatcttg
     9301 ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt ttgaaagaag
     9361 aatatggtct agccatagga gatacagtta agttatctgg tagtgacaag acgtttaaac
     9421 taactggctt tacggatcat gccaaattca atgtctcacc agttctctat acaaccatca
     9481 atgcttacca agaaatccgc ttcgaaaaag aagatacaag tgaaaatgca cggattaacg
     9541 caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag atcaaaattt
     9601 ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt ggatttatga
     9661 tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat gtactgacga
     9721 tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga ggatttattg
     9781 cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt cttggtctat
     9841 taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa agcaattggc
     9901 tgttttttgg cgtcattagc ttgttgatgt tagtagtagc tgtgttaggc gcactatttt
     9961 ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa ggaggaagaa
    10021 catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg accaagtagt
    10081 taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg caatcatcgg
    10141 tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc agacgcctag
    10201 cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa gcgaacgcgt
    10261 taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg ttccttttct
    10321 gacagtaaaa aagcagttat tattagtaga taagattaaa catgaacatc gtcaaaaaga
    10381 agcaaacgaa ttatttgagc agcttggcgt ggataagttg atcaacaagt atccagaaga
    10441 actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca atgatccgtc
    10501 cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac gagaagtagt
    10561 cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg ttactcacga
    10621 tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg tattgaaaga
    10681 aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa gatacacagc
    10741 aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc ggcacttcaa
    10801 caagagaaat cgcacaaaaa gttgggatca cccagcccaa tctctatcat tatttcggtg
    10861 ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt ggaaaagctt
    10921 tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca aaaatggcgc
    10981 agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat cttgaaagca
    11041 atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat tatcgtgaac
    11101 cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt tcaaaagaaa
    11161 tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg aaaagacctg
    11221 tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt gtattaggag
    11281 aagcgtaaag ctgttatata gggagaaaat agttaggtga agaagcaaga tatgacgaag
    11341 atcgttatat cttgcttctt tatataggta gtttttcata aatacaaatt tgttattctc
    11401 tcatgattat cagggtatat ttttatttat tatgtcggtg ttccaggaag tgttttaatt
    11461 taataacgaa gaaattaatt gtttgtagaa tgaaggaaac tatttattgg gtagatgaat
    11521 caccacgaat agtcaaaagg agatttcagg atatgccaaa aatgaaaaaa atcattttag
    11581 cagtaatgtt attttctacg gttatatctt ttacagcccc ttttttgctt acttaagctg
    11641 attttaagtt ttggccctta gtctggatct acgaggtgag agtcaagaat gaaaaaaata
    11701 attggagctt ttgtatttgc aatcattatc attattatct gttttcaaat agatgttttt
    11761 ggtcaaaaaa agatagttgg acaaacggtc agcagtacga acagtacggt tacaaagaca
    11821 aaggaatcat ttgatgatac agaacaagag acattaatca aaatcggaaa aaatgaccgt
    11881 acactatata cagatgactc gctaactacc ccacttgcaa ctataaacgg cggagagctg
    11941 gcggagtttc tttctgaaac aaaaaatgct tatcagatca agacaaatga tggctatact
    12001 ggatatttag ccctcgaaga tggaaccaaa gtcacaaaga atatacaaac aaaaccggaa
    12061 gagctatctg atgcagtgat tgtattagat cctgggcatg gcggaaatga tactggggca
    12121 ttaagcaatg atgaacaaac agaagaaaaa gatataacat taagtacagc cataaaagta
    12181 aaaaaggcgc tagaggatgc tggagcaacg gtttatttga cccatactac agatgaatta
    12241 gtacagcttg gagatatatg cgactacagt gaagaaaaaa aggcagatgt ctttatcagt
    12301 ttacacgcag actctacgga atatgcgaat gaagcaactg gaatcacgac atattattac
    12361 tatggacaag aggaaacatt agcacagaca attgctgact ccttcactga tctgcctctg
    12421 gattcaagag gaatctccac tgggaactat caagttcttc gagaaaatct tcaaccttct
    12481 attttaatcg agatgggtta tatgaataat gatagtgatt taaaggaatt agtaacagac
    12541 agttatcagc agcagattgc tgcaagtttg acacaagcat tgaccactta ttttcagtaa
    12601 taaataagcc cagccaaaca aaaatagctc ctcatatttt tcgtttggcc gggcttatta
    12661 tcgcagatgc aagcttttaa acaccgttta aggaagaggt cgtgagcctg ccgttcaact
    12721 ctgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgatgact attgtgactt
    12781 gtgaccgagg agttggcatg agaacaccgt ttaagaaaaa ggttgtgaaa tcaacgtttt
    12841 gttctgagta ttagagaaac aaacggaaaa atagttcctc atattttgcc gtttgttgag
    12901 ctaataccga ggatcagatg attgaacacc gtttattcgg aacaagggac tgtgacatga
    12961 cttttgtcac agttccatta tttttttagt agcttcaaag ctttttctgt attggcaaaa
    13021 tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag ttttgccgct
    13081 acttggtcag atttcgttcc agctcctgac ggaagggtga ttccagcttc agctgatgct
    13141 ttgtctaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc aacagctaaa
    13201 tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt tttttctaaa
    13261 cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat cgcttctggt
    13321 ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa agcgactttc
    13381 atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga taagacaagc
    13441 agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg gctatctttt
    13501 aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc acgtttcaca
    13561 taagcagcac atactgttag cggaccgaaa tagctgccat ttccgacctc atcactacct
    13621 aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc tggcttggct
    13681 tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt accttgaaac
    13741 atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc agcaaagcta
    13801 gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat ttgactaagt
    13861 tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt tttatacata
    13921 aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca tagcattttt
    13981 ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa cggtgaaaga
    14041 accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg attcgtatgg
    14101 cgcacgaaaa aacacgctac aaagcggtga ttgccaacca aacatatact attatcggac
    14161 gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag ttagcagaat
    14221 tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg gctgtcaatg
    14281 cgctttcgga tcaattgaag aaacaagaaa gaatattgga gctggaagaa gaaacagcag
    14341 aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa cgcattgaag
    14401 caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat cataccatta
    14461 aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa attaaacaaa
    14521 aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt tagaagagaa
    14581 aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg tttttttctg
    14641 gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta ttgtcattta
    14701 ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac attccatacc
    14761 cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgcc tttcacctag
    14821 atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc tggctgctta
    14881 cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg agacaggtag
    14941 actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata tttttgattt
    15001 tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc gagcatagtt
    15061 tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat gagctttggg
    15121 taacgaatat tttgaattaa ggaaaatagt gctggaaaat atttcgtttt ttgtacagag
    15181 tagtttattt aagaaggctg cgtagcgtat gtccggcaca gctctttttt catagtgaaa
    15241 gataaggtga taaaatgaat aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac
    15301 agatggtcag acaatttgta gtgactgcac aaggaaaaga agaactggca gaattggtac
    15361 cagtgagtga gaaacaaacg atcacaaact ggcttcaaga aactgaggat ggattgaaag
    15421 tgcaacgtct gcgaggcggg atccctatac ctaaaattga aaatatccgt ccccatatga
    15481 aacggattga gatcggagca gacttgaatg gagtggagct tgcacaagtt tccagagtat
    15541 tgtctacgac aagcgaattg aatcgtttca ttgatgatct ttctgacagt gaaatcgaat
    15601 ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat ccctgttttg agccgccgtt
    15661 taaaggaagc aatcgatgaa gacggacgcg tgactgatga cgcttctcct gagttaaaga
    15721 gcataagaca gaacattcgg cgaagtgaac aagctgttcg tgaacagtta gatgggatcg
    15781 tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat caccatgcga aatgaccgct
    15841 acgttatccc agtcaaacaa gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa
    15901 gtgcttcagg tcagacttta tttattgaac caaaacaagt ggtggatttg aataatcgtt
    15961 tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg tattttaagt gaactttctg
    16021 ctgaacttgt tccccatcgt caagaaatca tccacaatac ttatgtgatt ggtaaaatgg
    16081 atctgatgaa tgcaaaagca cgatttggta aagaagtaaa agccatcgtg ccgggaatca
    16141 gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc actgatcgat caggaaaaag
    16201 tagtttctaa tgacatcaca atcggaaaag actatcaagc catcgtcatt acaggaccta
    16261 acacaggtgg gaaaacgatc acgttgaaga ctttaggtct gttacaactg atggggcaag
    16321 caggattacc gattcctgca ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg
    16381 cggatatagg agacgaacag tcgattgagc aaagtctatc gaccttttct tctcatatga
    16441 cgaacactgt agatatttta tcaaaagtca atgaaaaaag tctggtatta tttgacgagc
    16501 tcggtgcagg aacagatccg caagaaggtg ctgctctagc aatcgcaatc ttagatgatc
    16561 ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta tcctgaattg aaagtttatg
    16621 gatataatcg ggcaaatacg attaatgcca gcatggagtt tgatgtggat acattaagtc
    16681 caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc
    16741 gattaggtct tgatacagaa gttattgatg aagcgaagca gttgatgaat gatgagagcc
    16801 aagatttaaa cgaaatgatc acagatttag agaatcgccg gaaaatggca gagaccgaat
    16861 atctggaaat gcgtcatttt gtttctgagg cacaagaact tcacgatgat ttgaaagaag
    16921 cttacagcta tttctttgaa gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa
    16981 atgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa
    17041 tgcaacagca gattgggcaa ggaaatgtga aagagcatca gttgatcgat gcaaagaccc
    17101 aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa taaagtattg aagaaggcga
    17161 aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt tacgacttac ggccaaagag
    17221 ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga aatcggtata ttaaaaatga
    17281 atgtgtccga agacgagctg acgcctgttg ctcctcaaaa agagccgaca caacgagtta
    17341 tccatgctgt acgatctgaa tctagtagtc acgtgccgaa tcaattggac cttcgaggaa
    17401 aacgatacga agaagcgcta agtgaggtag atcaatattt ggattctgcc attctagctg
    17461 gttatcctca agtgactatt gtccacggaa aaggaacagg cgcacttcgc aagggaatta
    17521 ctgattatct taaaaaccat cggagcgtca aaagctttga gtttgcaccg gcaaaccaag
    17581 gcggaaatgg tgcgacaatt gttaaattta agtaaagctt actaaaggta agcagatggt
    17641 tcgctatcca agtatagaaa tgctatacta gtcatagatt ttaaaaatgg aggtctttga
    17701 tatgtcacaa gtaattacag ataagacatt taatgaagaa acagataaag gacttgtatt
    17761 gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg caagcaccga ttttggatca
    17821 gttggaacaa gaatatgatg aagaagaatt cagaattgct aaaatggatg tggatgaaaa
    17881 tccagaaact cctcaacaat tcggtatcat gagcattcca acattaatgt tgaaaaaaga
    17941 tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat
    18001 cgatcaatat ttataaaaac ttacagcaaa aataagctga gggtgggact gaatccagtt
    18061 ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa attaatattt tttgagtata
    18121 attacgttgt caggctggat tgtttttttg acagacatgc tataataact ttaattgaat
    18181 atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa attagcttta cttcctgatc
    18241 agcccggctg ttacttgatg aaagataaaa atggaacgat catttatgtc ggcaaagcca
    18301 aaatattaaa aaatcgtgtc cgctcatatt ttactggtag ccataatacg aaaacggaac
    18361 ggcttgttag tgaaatcgaa gactttgaat acatcgttac agaatcgaat atcgaagcat
    18421 tgctcttaga gatcaatctg ataaagaaaa atgatcctaa atacaacatt atgttaaagg
    18481 atgataaaac ctatcctttc cttaagataa cgaatgaaaa atatccccgt ttggtgatta
    18541 ctcgtaaggt actaaaagac aaagcactgt attttggacc atatcccgat gtcggtgcgg
    18601 cgaatgagac aaagaagatc ctcgatcgat tatttcctct gagaaaatgc aaacctagtc
    18661 agacgaagga gccttgtctt tactatcatt tgggacaatg cctgtgtcct tattatttcg
    18721 acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt gaaaaacttt ttaaacggag
    18781 gacacgaaaa aatcgagtca gagatccatc agaaaatgga aaaagctgct gaaaacatgg
    18841 agtttgaaaa agctgcagaa taccgagacc agatccgtgc cattgagaca atcatgactc
    18901 gccaaaaaat gacaaatact gatttactgg atcgagatgt atttggttat gcgattgata
    18961 aaggatggat gtgtgtccaa gtgtttttcg taagacaagg aaagctaatt gaacgagatg
    19021 tttcaatgtt tcctttctat aacgaagcag aagaagattt cttgacttac ctcggtcagt
    19081 tttatcagga aaatgagcat ttcattccta aagaagtctt gattcctgat acgatcgaca
    19141 agcccagtgt agaagctttg ttagctacga aagtgctgca accaaaacga ggagaaaaga
    19201 aaaaattagt tgaattggcg agtaagaatg cctcagttgc tttgaatgaa cggtttgatc
    19261 tgattgccag aaaacaggaa cggacgatcg gagcagttga acgtttagga aacgcgatga
    19321 atatcccgac cccaatacgt atcgagtctt ttgataactc taatatcatg gggacgaatc
    19381 ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat
    19441 ataaaataaa aacagtcaac ggaccggatg attatgcatc aatgagagaa gtcatctatc
    19501 gcagatattc gcgagtgatt cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg
    19561 gaggaaaagg acaagtagat gtggcaaaag atgtcttaga aaatcaatta ggaatcgata
    19621 ttcctattgc tggcatggta aaaaacgata aacataaaac aaatgagttg ctattcggta
    19681 cagatttggc tgtagtaccg cttgagcgaa attcaccaga gtttttctta ctccagcgaa
    19741 tccaagatga agtccatcga ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca
    19801 gttttgcttc ccgattagat gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc
    19861 ttaaagagtt caaatcgttg aaaaatatca cagcagccac gacagaagaa ttacaagaaa
    19921 tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa attacatcag gaatgaggaa
    19981 agggcacttt acaaagaaag taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt
    20041 tacgttttgt tggtaaataa taaatcgctt acgtctggtg tctaaggata tatgtgattt
    20101 cttgaagaag gagtgaagaa atgttagaag tcaaggatct tgttaaaact tttgggaact
    20161 acacagctgt cgatcatgtc tctttccaaa tccccgatgg aaaaatcatg gggctaattg
    20221 gccaaaacgg ggctgggaag acaacgactt ttcgtttgat cttggatttt ttgacacaag
    20281 accaaggaga agtgttatgg aatggccatc atcttgggga gaaagattat gatgtcatcg
    20341 gttatcttcc ggaggaacgg ggactttatc cgaaagtaac gattcaagac caactgcttt
    20401 attttgcaga acttagaggg aaatcaagaa aagagattga accaaaaatc gacttttgga
    20461 tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt aaaatctttg tctaaaggaa
    20521 atcagcaaaa agtccagctg atcgccacgt taatccatga acctaaattg atcattttgg
    20581 atgagccatt cagcgggtta gatccggtaa atgctgaatt gctaaaagat ggaatattgg
    20641 aattgaaaaa acatggttca tgtgtcattt tttctagcca caatatggat aatgtagaaa
    20701 aaatctgcga tcatttgatc atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac
    20761 atgagattcg tgaatcattt ggccgtacga agttattctt ggaatctggt ttaagccaac
    20821 aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag
    20881 agattacctt agaagatcct gaagctggga aagaaatctt tactcgtgcg actcagttcg
    20941 gctacattcc tatgtttaac cagcaaccgc caactttgga agagattttc aaattgaaag
    21001 caggtgaaat cgatgcgtaa gttttggatt attaccaaag atgtctattt aaaaaatgtt
    21061 aagtcgattt ctttcttgat catgatatta gtccctttta tcatgctggg tatcatttat
    21121 gtagcaggaa actttgctca acaaaatagt gatgtcaata aaatcggcgt attggctgat
    21181 aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag atgattttga gttcaaagca
    21241 gttgattctg aagaaaaagc acaaaaagag ctttcggaag aaaaaatcga tgcgtattta
    21301 gtagtctcaa ctgagaatga taagataaat gggatgcttt atagcgaaaa ttcattagga
    21361 caaagtacac aactactgat acaacaacaa ctgactggct tgcaatcgat gcttcgagcg
    21421 agtcaattag gcatttcacc agaagaagta gctagcttga gccaacaagc aagtttgact
    21481 cgacaaaaag tcaattttga ttcaaaagga aaaatgacag tcggagaaga taactcgaca
    21541 gtccaatacg tcgtaagtta tgtagcaacg attgtcttgt tcattattat tttgacgtat
    21601 gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc
    21661 ctgtccagta cgactgcaca aaagcatttt tacggaaaat taacaggtgt tttacttgtt
    21721 gcattaacgc aaatggcatt atacggtatt atttttggta taggatacag tcaattcaaa
    21781 gatatggaaa tggtcaaaaa tatattagac ggtatctcat tggataactt gattggacca
    21841 ttcttgtggt attctcttct attcatgatc tttggcattt tgattttttc tgttttagca
    21901 gctttatgtg gctctttggt caacaaagca gaagatacag caaaagcaat tttgccagtg
    21961 acttatctat ctctaggcgg atacatgctt ggattgatac taggagcttc agatccaaac
    22021 aatatcgtta ttcggatcac ttcgtatatt ccattcttat cttcatatat tatgccgatt
    22081 cgcttagcaa atgagacagt cggtatgggt ggagcattga tttccttgtt ggtacttgta
    22141 gccgtaacat tcttattgat gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtt
    22201 tatagcgaag gcggattatg gagttcattg aaacaatcga tttcaattat gaggaatgaa
    22261 cgaaaaaaag cataaaacaa tcctaaaata cagaaggaag ctggacatct attgttccca
    22321 gcttcttttt gtatagacct attttattta ttttactata ttggatatat attcattgaa
    22381 aatagtgtat aacaacttta taatattgag tataccaatt tttggaggcg ttttaatgga
    22441 ccgttattta gatttacttt cagaaaaatt cccctcaacg gaggatgtca tcacggaaat
    22501 catcaatctc gaagcaattt tgcagcttcc aaaaggaaca gagttatttt tgagtgatat
    22561 acatggcgaa tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga
    22621 aaagatcaaa gacctttttt ctgaacaaat gacggaaacg gatatcagcc aattcactat
    22681 ttttacggct tatccagaat atgcattaac gactgattgg tacaaagaac aagataaatc
    22741 gcagctgatc catcatttga tcgatctttt acggtttacg acagtcaaat atacccggtc
    22801 aaaagttcga aaaggattgc cgaaagaata tagctatatc attgaggaac tcttgtatat
    22861 cgatgaccgt atcaatggaa aaaaagacta tgtaaaaaaa atcattgaac aacttgtttt
    22921 gatgaaggaa gaagaaaaat ttttaacgaa acttgcacag acgatccaaa aatctgtcat
    22981 cgatcatttg catatcgttg gagatatttt tgaccgagga actcaagcag ccaaagtgat
    23041 ggatcgaatc atggattttt cttctgtaga tatccaatgg ggaaatcatg atattttgtg
    23101 gattggtgcg tattgcggct ctgaggcatg tttactgaca cttttacgga ttgcagcacg
    23161 ctataattat ttatttgaat tagagaaaga atatgggttg aatttacgtc cattgttttc
    23221 ttttgctcat caaacttatc agaagaatcc agtatttgcg cctaaaaaca gtaaaaatct
    23281 atccgaaaga gagcaagaag aattagaaaa aatccatcag gcactttcta tccttcaatt
    23341 caaatcagaa catcagttat tggatcgtcg acctgaattt aaaatggacg atcgtaaatt
    23401 gctggaaaaa atcgactatg aagcatcaac gattgattta gaaggtcagc tctatgagtt
    23461 gaaaggaact tgttttcaaa cgatccagcc agatgatcca aaaaaattct tggaagagga
    23521 acaaaaagtg attgatagtt tgatgtattc attccagcac tctttgagaa tgcaaaaaca
    23581 tatgacgttt ttgatcgaaa aagggggaat gtatctcacg aacaatcagc atgtgctgtt
    23641 tcatggctgt atacctgtag acgagaaggg acagctctta gcatttgaat tagaccaatc
    23701 ttatcgcgga aaagaactgc tgggcttttt tgaaaaacag ataactgaaa gcgcaaaaga
    23761 tctgactgca aaagaagatt tagcaaccga tttgatttgg tatgcgtgga gtgggccatt
    23821 ttcgccttta ttcggcaaga gtaaaatggc gacttttgaa cgttattttc tcacagagcc
    23881 tcatacgcac gtcgagaaaa gcaatcctta ttatcacttg cgagatgaag agtggttcag
    23941 tgaaaaaatt ttagcagaat tcgacacaaa agaagaaggg tcagcaatca ttaatgggca
    24001 cacgccagta aaagtaaaaa aaggggagtc accgatcaaa gcaaatggaa aagtatttgt
    24061 cattgacgga ggtttatcca aagcttatca aaaaacgaca ggaattgccg gttattcact
    24121 attatgcaat tcttatggtt tccaaatcgt gacacattat ccgtttttgc ctattgaaca
    24181 acaatttgca aaaaaatccg accaaacaaa tgtgaaaaaa gtaatcgaac agcaactacc
    24241 acgcaaactc aatcgtgata caacaaaagg tatggaactg cagcagcaga ttacccagct
    24301 caaaaggctg ttggactatc gaaaggacga ttgagaagaa ttccagtgaa cattgttcca
    24361 tagactggag actgagacaa aagtcgtgtc gcagtctctt tttccggata aacggtgttc
    24421 ttctgccaac tcctcggtca caagtcacac tattcaacaa acatgaaaag ctgttttttg
    24481 aaattgctcc ttgtgactca gagttgaacg gcaggctcac gacctcttgg tataaacggt
    24541 gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtga aaagccattt
    24601 tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacaacccc ctcagaaagt
    24661 ctattaaaat tcattcacaa agaaatcgaa taagcgctgt tcagatggat gtacacggtg
    24721 ggtaagttcc ggatgccatt gaacaccgag gattttatgc atatcatctt tgctttgaac
    24781 cgcttcaacg agtccatcgt tagaaaaagc gatggctttt aataaaggtg aaagatcttt
    24841 gactgcctga tgatggtaag aattcacaaa atatggatcg gggagaaggt tgcttaacaa
    24901 actgtctgaa acaatcttga cttcgtgagt cgtaaattgc ggggcagtgg gctgttgttc
    24961 atgttttact gaccactttg gatataatga aagatcttga taaagtgtac cttccaaaac
    25021 gacattcaat agttggagtc cccggcagac tgcaaaaatt ggttttcttt gtttcaatgc
    25081 ttccaagatc agtgcctgtt caaattggtc tcgttgaata tttgtttccg ttagctttgg
    25141 atgaggatct tccatgtaaa gttgtggact gacatcttga ccacctgcaa gaataagttt
    25201 gtcgatttgt ccaatcagtt gaggagctga atctggagac atgactggca gaataagagg
    25261 caatccttga gcatctaaaa ctgcatccac gaatccttgc ggtgtatagc tcacttgatt
    25321 tccttggaaa gtatctgttg ctcgaatcag ttgatttccg gcaatgccga tgattggttt
    25381 catttgtatt ccccctaaag tagacttatt gtaacaagga tttaaaaaaa attaaactat
    25441 gtaagacttg cccctatact tatctgttct tgctgtgtta ctatctgtaa agagaaattt
    25501 cgtgtgaaat tcatgacgat agttgaaaat attgcttaat ttgttataat aaaaagtaag
    25561 cagaaataaa aaggttgggc gtaagcttca aggatctagt tcttcaaggg gtgagaagga
    25621 cattttattg aaggggcctg cctatactaa gttatcttta gaggtacata ctttcaatga
    25681 aaaaagaaag catgtacctc ttttttaaat cgaaaggaag tgaagcaagt gttagaagct
    25741 ttggaacaaa tccgaaaagc agaagaagaa aatgaaaagc gtgctcaaac actgcggcag
    25801 cagctgaaga tctatgcaac agaaaaagag caggaattgg aaacgatcca aacaaactat
    25861 cgacaacaac taaaacagtt ggtggaagaa aaagaacaat ttgaaataca agaattagaa
    25921 aaagaaaaaa tgaaattgaa agcagaagca gacaaggtgc aagcaacttt gaaagatcaa
    25981 tatcaaaagc atcagagtca agcgatcggt gcgatcattg aaagggtgaa agaaacttat
    26041 ggccgtcact aagatggaaa aagtgaccct catttcagat aaaagaaatc aggagaggat
    26101 tttacaagca gtacaaggaa tccaaaacgt tgaaatccgg gatctgtatc aaaatagaac
    26161 aaacaaccag tgggtggaga agtattttcc agataccacg attattgata aagaagcaaa
    26221 attaagcgct ttaggtaatc ggttgaaaga catccaagaa gctatccaat tcatacaaca
    26281 ccacggcgat agccgtcaaa agaaactcca tttgaaacgt caagaacttt ccttacaaga
    26341 tttggaagcc aattattccg aagaaaattt cataaataaa ttgaaagaag tccttgattt
    26401 aaaagaaaag tgggaacaac taactgaaga acgtgatcgt ttagaagaag aggaagaatg
    26461 gcttcttcat tggcaacagc tggatattgc tccaagtatg tatgcaagta agacgaccat
    26521 ttttgagatg gggactgtga gtgcagcaaa ttttgaaagt ttcaaggaag aactgagtaa
    26581 gcttgatgac gtttatatgg aagaaataga ttatacgcca aaacaagttt atctttcgta
    26641 tgttgcttta aaaaaatcag ttgctgttat agaagaaatt gctgggaaat acgggttcaa
    26701 taaagaaacg tatccttatg aacaaaatcc taaagaaaaa ctgcaagaga ccaagcagca
    26761 attatccatt gttcacgagc agcaaaagaa gataacgaca gcgttaggtc agtgcagtgg
    26821 ctatatccaa gaatttgaat gggtagaaga agtcacactt gccgttgcag aacgagaaaa
    26881 aataaaagaa cggtttatcc atgcgactta tctgatcgtt ttgcaaggtt gggtagatgc
    26941 agaagaaaaa cagaatttgc tgcatgtgtt aaacgaaacc gtatcagaag aagatatata
    27001 cgtcagtttc gaaacaccaa actcagaaga gattcagcag gaagtcccta ctaaattgaa
    27061 aaaccatccg cttgtcgaac cttttgaatt gctgactgaa atgtacagtc tgccaaaata
    27121 tgaagaagta gatccaacac cttggatgac accattttat cttgtcttct tcgggatgat
    27181 ggtcgcagat gttggttatg gtttgctgat gctcattggg acaatactag ctcaaaaact
    27241 acttgttcta ccacgcggga tgaaacgttt cgtaaaattc tttgagattc tctcgatacc
    27301 atcgatcatc tggggattga tctacagttc atttttcgga cagtcattac caaaagaact
    27361 gtttggcatc cgcttgcctt tcccaatcct atctacgacg gatgacgtca atacgatttt
    27421 gattttatcc gtgattttcg gtctgataca gattttggtt ggattatttg tggcagcaaa
    27481 ggaaaatatc aggagaaaag attacttgag tgctgtcagt gatggatttg cctggcaagg
    27541 gatcctaatt gggatcgtta ttgcgttggt tggtgccatg ttgataaaaa atacagcatt
    27601 tgtttacctt ggcgctgggc tatcgatcat tgctgcctta tgtatcttga tcgttccgat
    27661 tatccaaacg ccgtctaaag tcaaaggtgc tgccaaaggt gcgtataatc tttacggact
    27721 gaccggctat atcggtgatt tagtcagtta tacacgtttg atggcattgg gtatttcagg
    27781 cggaagtatt ggagcggcat tcaatatgct agttgctttc atgccgccag ctgctcgatt
    27841 cagtgtaggg atccttttga tcatcgcttt acacgcactg aatatgtttt tgacactgct
    27901 aagtgcctat gtccatggcg cacgattgca gtatgtcgaa ttttttggga agttttatac
    27961 aggaggcgga cgtgcctttg atccgctgaa aacggcggaa aaatacgtca acatcaatca
    28021 caagaaaaaa aacaataaat aattggagga tttttatata atgatggatt acttaattag
    28081 tcaaaatggt ggaatggtat ttgcggtatt agcgatggca acagcgacga ttttttcagg
    28141 gatcggttca gctaaaggtg ttggaatgac aggtgaggca gcagcagcat tgacaacaag
    28201 tcaacccgaa aaattcgggc aggctctgat tctgcaattg cttccaggca cgcaaggttt
    28261 gtatggattc gttattgcct ttttgatttt tatcaactta ggaaatgaca tgtcagttgt
    28321 acaaggactg aactttctag gtgcttctct gccgattgct tttacaggct tgttttcagg
    28381 tattgcccaa ggaaaagtcg cagcggcagg tattcaaatt ttagcgaaaa aacctgaaca
    28441 tgctacaaaa ggaatcattt ttgctgcgat ggttgaaaca tacgcgattt taggtttcgt
    28501 tatttccttc ctattagtgt tgaatgccta agcataaagg aggaaaagca gatgaacgcc
    28561 attgaaaaaa tcatctctca gatgaacgaa gcagcagagc aggaaagagc ggcgttagaa
    28621 caagaagagc gaatgaagat cgatcaaaac tttgaacaaa aacgtacgca agttgaaaca
    28681 gaacatcaaa aacaaaaaga aaaacaaatc gaattactcg aaaaaaaata ccgtcagcta
    28741 aggaatcgcc agcaagtaga agtacgacaa gaaaatttga atgcgaagca ggaattttta
    28801 agacgattgt ttgcagatgc ggtaactgaa atggaaaatt gggatgaatc tgaacaaatc
    28861 cagtttatca aaaatgcatt gtattcatta cctttgacag gaaaagtagc attcattgct
    28921 ggagaaaaat cagcagccta tctttctcag acgttgttgg atgaatggaa caatgaactt
    28981 ccctttatga tggttttaag tgatgaaact gttgctgatc aagcgggttt tctgataaac
    29041 gatcaaggag ttcagtataa ttttcttttc agcagccttg tacaagatat ccaaggaacg
    29101 atgagttttg agattgccaa tcaattattc gaataaggag ggatcagtgt atggaatacc
    29161 acgaactaaa tcccttgatt cgcggaagag agcttgaact tttgtccaat gagacatttg
    29221 aacggatgat tcaaacaaat tctgtcgaag cacttggtga aatattgaaa tccacgattt
    29281 acagtccata tatttatgac ggatatgaat atgacttcga acagaatctt ttaaaggagc
    29341 agagccgtct atttcgctgg ttaaaggaat cagcaccgga gcctgaaatc gtttggatct
    29401 atacgatgcg ttatactttc cataatctga aggttttgac aaaagctgag atgacaaatc
    29461 aaaatctgga ccatttgtat atcgacgatg gattttattc attagaaacc ttgaaggatg
    29521 ccattcatac gcaagtttca aatgaacttc ctgaaagcat tatggacagt atacgagaag
    29581 tacgtgatta ttttgaagag tctaccattc ttcagggaat tgacgtgatt tatgatcgac
    29641 agtttttgac agagcagcga cggcttgccg ataagctagg atatcaagaa ttgctgacag
    29701 aagtcattgc ttttattgac ttgaccaata tcacaacgat ggctcgagga atattgcaga
    29761 aaagaacata tgggttcatg acgacggtac tttccagctc gggaagtatc gaaaaagaag
    29821 tatttttgaa ctttgtagat aaagatatgg aagattacac gaattttcta ttagagacag
    29881 attatgctga ttttctgcgc cctgtcatcc gtgagggaaa tattgatctc gtaaagcttg
    29941 aacagttgaa agatgattat ctaagttcat tgtatcaaga agcacaaacc caggctttcg
    30001 gaccactgcc actactagcc tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt
    30061 tactggttat cggcaaaaga agccatttcg aatcagaaac aatcagaaag cgggtgagac
    30121 aagtctatga cgcataagat cggtgtagtt ggcgacaaag attctgtttt gccattcaaa
    30181 ttgtttggct ttgatgttcg ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa
    30241 gaaatggcaa agaatgagta tggcgtgatt tatgtcacag agcaatgtgc agcattgagc
    30301 ccagaaacga tcgaacgtta caaggaacaa atgatacctg ctatcgtatt gattcccaat
    30361 catcaaggat cattagggat agggctggaa gaaattcaaa aaaacgtaga aaaagcagta
    30421 ggtcaaaata tcttatagta tgaaaaggag atagaaattt tgcaaattgg aaaaatcata
    30481 aaagtctccg gacctcttgt aatggctgag aacatgtctg atgccagtat ccaagacatg
    30541 tgtctagtgg gagatctagg agttatcgga gaaattatcg agatgcgtgg cgatgtggcg
    30601 tctattcaag tatatgaaga gacttctgga attggtccag gcgaaccggt tcgctctact
    30661 ggtgaagctt tatctgttga attaggacca gggatcattt cacaaatgtt tgacggaatc
    30721 caacggccat tagatacctt tatggaaatc acacaaagta atttcttagg gcgcggagtt
    30781 caattgcccg cattggacca tgagaaaaaa tggtggttcg aaccaacagt agaagccgga
    30841 gaaacggtga gtgctggaga tgttatcggg atcgtagaag aaacaaaagt tataaaacat
    30901 aaaatcatgg tgccaaatgg gataaaagga acgatcaaaa caatcgaaaa aggttcattc
    30961 actattgatg aaacagtatg tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg
    31021 cttcaaaaat ggccagtaag acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca
    31081 cccatgatca ctggacagcg agtgattgat acattcttcc cagtgacaaa aggaggagca
    31141 gcagctgtac ctggtccttt tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa
    31201 tgggccgatg tggacatggt tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg
    31261 gatgttttaa atgaattccc tgaattgatt gaccctaata caggagaatc gttgatggaa
    31321 cgtacggtat tgattgctaa tacatcaaac atgccagtag cagctcgaga agcttctatc
    31381 tatacgggaa tcacaattgc ggaatatttc cgtgacatgg gttatgacgt tgctatcatg
    31441 gcagactcca cttcacgatg ggcagaagca cttcgtgaga tgagcggacg tttggaagaa
    31501 atgcctggtg atgaaggtta tccagcttat cttggttcac ggcttgcgga atactatgaa
    31561 cgatctggaa gagtcattgc attaggatca gaaggtcgcg aaggaagtat aaccgcaatc
    31621 agtgctgtat ccccgtctgg aggggatatt tcggaaccag tcacacaaaa tacattgcgg
    31681 gtcgtaaaag tattctgggg tcttgattcc agtttagccc aaaaaagaca ttttccttcg
    31741 atcgattgga tacaaagcta ttctctttat tcaacagaag tagggaagta tatggatcat
    31801 atccttcaag ctgactgggc atccatggtc accgagggca tgcgtatttt gcaggaagaa
    31861 cagcagctga atgaaatcgt gcgtttggta gggattgatt ccctttcaga taatgaccgt
    31921 ctgacattag aagtggcaaa atccattcgt gaagattatt tgcagcaaaa tgcttttgat
    31981 gatgtcgata cattcacttc aagagagaaa caattcaaaa tgttgaagat gatcttgacc
    32041 tttggtgaag aaggacgccg tgcgttgtcg ttaggtgctt atttaagtga gatcatggac
    32101 ggtaccgtat cgattcgtga aaggatcgga cgaagcaaat atatcccgga aacagaatta
    32161 gaaaaactag atcaaattat tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa
    32221 ggaggcatga gcaatgatta aagaatatcg tacgatcggt gaagtcgtcg gtccattgat
    32281 ggcggttgaa aaagttgctg gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa
    32341 tggcgagatt cgtcgtggac aagtattgga agtccaagaa gataaagcaa tggtccaaat
    32401 attcgaagga acaagtggta tcaatttacg tgactcttct gtacgatttt taggccatcc
    32461 tttagaacta ggtgtatcgg aagacatgat cggtcgagta ttcgatggtt tgggacgacc
    32521 gaaagataac ggaccagaga tcctgcctga aaagcatttg gatattaacg gagaagtaat
    32581 caatccagta gctcgggatt atccagatga gtttatccaa acaggtatct ctgcaatcga
    32641 tcatttgaat acattagttc gtgggcaaaa attaccagta ttttctggct ctggtcttcc
    32701 tcataaagaa ttagctgctc agatcgcgcg acaagcaacg gtactcgatt catcagatga
    32761 tttcgccgta gtctttgctg ctattggaat cacttttgaa gaagctgaat ttttcatgga
    32821 agattttcgt caaacgggcg caatcgaccg ttctgtcatg tttatgaatt tagcaaacga
    32881 ccctgcaatc gaacgtatcg ctactcctcg tatggctttg actgctgcag aatatcttgc
    32941 ttatgaaaag ggaatgcatg ttttggttat catgacagac atgaccaact atgcagaggc
    33001 attgcgagag atttctgcag cacggcgaga agttccagga agaagaggat atcctgggta
    33061 tctttacacg aacttagcta ctttattcga acgagcagga agaatccgcg gactaaaagg
    33121 atcggttact caaattccga ttctaacaat gccagaagac gataaaactc atccaatccc
    33181 tgacttgact ggttatatta cagaaggaca gatcatcttg actagagagc tttacaagag
    33241 tgggattcag ccaccaattg atgtactccc gtctctttcg cggttaaaag ataagggaac
    33301 tggacccgga aagacaagag aagaccatgc agctacgatg aatcagcttt ttgcagccta
    33361 tgcacaagga aaacaggcaa aagaattagc cgtagtttta ggtgaatctg ctttatctga
    33421 tgtagacaag atctatgcga agtttgctga gcggtttgaa aaagaatatg tcaatcaagg
    33481 attctacacg aaccgttcga ttactgaaac acttgatcta gggtgggaac tgctatcgat
    33541 gttgccaaga accgaattaa aacgaatcaa agacgacatg ctggacaaat atctgccaga
    33601 agggaagtga gttctgatgg cacgattgaa tgtgaatccg acccgcatgg aattgactcg
    33661 attgaaaaaa cagttgacaa ctgccacaag agggcataaa ttattaaaag ataagcaaga
    33721 tgaactgatg cgtcaattca tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat
    33781 ggaagagtct atgacagaag caatgtctga ttttgtattg gcaaatgctt cgatcacgga
    33841 acctttcatc gaagaattat ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga
    33901 caaaaatatc atgagtgtaa aagtacctgt gatgaatttt gaatatgatg aacagctgac
    33961 agacacacct ttggaatacg ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga
    34021 tcggtttact accctgcttc caaaactttt ggaactgaca gaaatcgaaa aaacttgtca
    34081 actgatggcg agtgaaatcg aaaagacacg tcggcgagtc aatgcattag aatatatgac
    34141 gatccctcaa ttagaagaaa cgatctatta tattcgtatg aagctggaag aaaacgagcg
    34201 cgcagaagta acaaggttga tcaaagtaaa aaatatgggt accactgact aagtggtatc
    34261 tttttttaaa agaggaaaac gatgattaaa caaagcaaac aaataaagaa acgtttgccg
    34321 gctgttcagc ttctcgcatt aggatttttt ctcctgattt tagttggcgg cggcctactt
    34381 accctaccat tttttagtaa tagtgggcat gggacaaatt ttatcgatgc gcttttcact
    34441 gcaacttctg ctgtctgtgt aacgggattg acaacattga atacagcaga gcattggaac
    34501 agtgctggtc aatttttgat catgcttttg attgagatcg gcggtcttgg ttttatgatg
    34561 gttccgattc tatgctttgc attgataaag aagaaagtca gtctcagcac acgtattata
    34621 ttaaaagaag cgctgaatct agaagaagta tcaggtgtga tgaagctcat gctttacatc
    34681 ctaaaattgg cctttgtgat ccaattgatt ggtacggttg ctttatcttt tgtatttgtg
    34741 cctgaatttg gctggttgaa aggtatctgg tacagtttgt tccatgcaat ctctagtttt
    34801 tgtaatgcag ggtttgattt gttaggagac agtttagcca atcaccagca aaatgtttat
    34861 ttgatcatgg tggtatctgc tttgatcatt tctggtgggt taggcttcat cgtatggcgt
    34921 gatttactgg aatatcatca gaaaaagaag atgacgattc attcaaaaat cgcattaacc
    34981 gttacaggta cccttttatt aggcggattc gttgtgttct ttttcacaga gcgcaatggg
    35041 gcaacgctag tggaagggaa tatgtttcaa cgattggcaa acaccttttt tatgagtgtg
    35101 acacctagaa cggctggata ttattcagtt gattacctcc atatgagcca tgcaggtctg
    35161 attttaacga tgtttttgat gtacatcggc ggaacatctg ggtcgacggc cggcggattg
    35221 aaaacaacta cgttaggtgt gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg
    35281 agagcggaag catttggccg taccatccga caaggcgcag tgttgcgggc acttacttta
    35341 ttttttgtta cgttgacgtt gtgtgtagtc gccatcatga ttttatcggt gactgaaacg
    35401 atacctaaaa cttctggcat cgaatatatt gcttttgagg tcttctctgc gtttggtaca
    35461 gtaggtctta cgatgggatt gacgccagac ttgactgcaa tagggaaatt gatcattata
    35521 gcgcttatgt acattggacg agttggtgta ttgactgtgg tcttctctct gatgacaaaa
    35581 gcacaaagaa cacaatcaaa atacaaatat ccagaagaaa gtgtcatgat tggataattt
    35641 catcttctga cagaaaccta gtgatttagt aaaataagaa aataaaataa tttttcttgc
    35701 ttcttggata ctttaagtgc aaaatgttcg tttatttttt gaggcactac ccgttgttga
    35761 acctcgggta gtgcctcttt tttattgaaa ggaatcaata agatttgatt cctttattta
    35821 tcaataggtt ataattcata tttttttatt ttttctttta cgatacttag tccagaagct
    35881 aaaatttcaa ggttattaga ggatagggaa attcgaacga atgcatctgg ttgcgcacgt
    35941 gtagtaaagc gtctggaatg ataaagtcgg ataccgttag caagaaagtc attctcaatt
    36001 atcttagggt caatctcagg atcaactgga agaattcgat agtaagggac acgattcgtt
    36061 ggtttttcta ctttgaaaag agaatcaaaa agctgattgg ctctttttgt aagacttaat
    36121 ttagtttcta agatcctatt tgcttcattt gaatggagga tttgagtgat gacttccaca
    36181 tctaatccag aagttttcac atttatatta aaaattgctt gttctatctt gctttggaca
    36241 tgtactggaa ctacaagata agcaactcgc aatccagaac aaataaattt tgtcattcca
    36301 gctaagtaga ttgtttggtt aggtaataaa ttttggaaag gaggaatgat cgtatgttga
    36361 tagtaagttg ttaaaaatga atggatatca tcttcaataa cccatagacc ttctttttca
    36421 atcacttcgg ctaattgctc tcgtcttttg tgagagattt gaaaccctat aggattatta
    36481 caagaaggca taagaaagac accatgaatc ttcttttttc tacattcctg aatcaatata
    36541 tcaggtcgca ttccttgctc gtcaaaatca attggaacga tctctaaatg atataactga
    36601 gccaattcaa tgaaattagc gtatgtatat cggtctgttg ctattctatc acctggagag
    36661 aaaagtgcag aaagtgtgat agccaatcca ttttgaacac cggaaacaat agatagagat
    36721 tctaaggacg gaatgactcc ttgagttttt aaccaatttg ctgcagtagt caattgaaaa
    36781 tttgtgcctt tgggatcttg gtaattaagc aaaccaacaa gatttgggtt attactagtt
    36841 gattgaataa acgggacaac gaatttatta cattcttcaa aagaactaac cagaccaaaa
    36901 tcaatgatat gttcggttac ttcattggta gagatggtga tggactcaaa gacattggga
    36961 gaaataaatg tgccacttcc aatattcgta tacagcaaac ctttctcaat tccatattta
    37021 taagccttgc caacggtagt aaaatttaaa tctaaataat cagctagctc tctttgggag
    37081 ggtaactgag tgtttttctg taatttattc tttaaaatat cttgttctaa ttgttgaatc
    37141 aacgatcgat aataaggacg cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa
    37201 ttgtcataag aattaatagg cattttctcc actccttaaa attgtatacc atacaatttt
    37261 accattgatt gtattgattt tagaagttaa tcttttgtga ggagggaatt gtctatgaaa
    37321 attattaatt ttgaggaagt agtagcttct tttagttttg aagaagcgat tcaagtaatg
    37381 aaaaaatgtt tttttgatta tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa
    37441 attttacctg acggaaaagg caaaaatatg tttgcgatga tgcctgctta tttgggggag
    37501 aacagatatt ttggcacaaa agtcattacc gcttttccgg ataatcatca gtatcattta
    37561 ccttctcatt taggagagat tttcttgttt gatgctcaaa acggactacc caaagcattg
    37621 ataaacgcaa atgcagtcac ctggattcga acagctgctg tatctgcgct ggcaacagat
    37681 tacttagcaa atccgcatgc agaaacactt gctttaattg gagctggtca acaagcattg
    37741 tcgcatttga ctgcgatttc taccattcgt cccatcacta cagtgtttgt ttacgatatt
    37801 ttagaagaaa gacgaaatac ttttatcgct aaagctaaaa aacagtatcc taaaataacg
    37861 tttataaatg caatatcagt agaagatgca gtacgtccag cggaaatcat ttgtacgttg
    37921 acctctagta aagaggcatt tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat
    37981 gctgtgggca catttactcc tgatacaaga gagataagta catcgttgat ggaaacttct
    38041 caaatctatg tagatgatta ctcagctgcc ttaacagaaa gcggggacct gcttatagcc
    38101 atggcagaag gagatttaga ggaatcttgt attgttggta cattaggcga attagtttct
    38161 gagaaaaaaa cattttataa aggagaatcg aggccgacga ttttcgacgc agttggtcta
    38221 gctattgaag atctttgttg tgcagaatat atttataaca aattagagaa aggggcttta
    38281 aaatgaactt agattattac gtagttgatg catttgcaga tgaggtgttc aaaggaaatc
    38341 cagcagcggt ttatgtgcta gaagaatggc tgccagaagg tacgatgcaa aaaattgcca
    38401 ttgaaaacaa tttatctgaa acagcattta cagtgaagaa aaaccaagaa tttgagctta
    38461 gatggtttac gccagatcga gaaatcgatc tttgtggtca cgcgacatta gcaacagcat
    38521 ttgttttatt taactattac aaaatacctg atgaaacgat taaattttct actcaaagcg
    38581 gtaatctatt tgtgacaaga attaaagatt attattacat ggattttcct agtattatgc
    38641 cgaaaaaagt gccgatattg tctgaatatg aagaagcgat tggggctaag attaaagaag
    38701 cctacttagc acgtgattta tttttcgtat tagaagatga aaaaacggtg gctaaattgc
    38761 agccagattt tacagctatc aaaaattttg aattaggaat aggagtcatc gtgacagcag
    38821 aaagtgtgca aaaagacttt gtttctagaa ccttctttcc aaaattaaca atcaatgaag
    38881 atccagtatg tggatcagca cattctaatc ttattcctta ttgggcagaa agaatgaata
    38941 aaacaaaatt gtcagcttat caaatgtcat caaggggcgg ggatctagaa tgtgaattaa
    39001 aagaagatcg tgtgataata ggaggaaaag cagttttgtt ttctaaaggt acctcgttta
    39061 tttgatttct ttcaaatgaa ttaaaaataa attggtggag agtggcaacc ttatagctac
    39121 aatggttgtt aaaaaaagac gcaaactttt tcaatttaat gaaaagtttg cgtctcattt
    39181 taattattgg tgtttgtttt ttattcaatc aagagtaaaa tgccccatac taactatcct
    39241 cggatgactt cgattttata accatcaggg tcaatgacaa agtagtaaga aggcgcagtg
    39301 ccaggtaatc cttttaagtc agtaatctca agaccagcag ctttatgttg ctcgtgtaat
    39361 ttttctagat catcagctgc tatagcgata tgaccatagc cgtctcctag atcatacgca
    39421 ccatgatcat agttgtaagt caactctaat tcatagtcat ccccaggaag tgtcaaataa
    39481 acaagtgtaa atttgttttc tggaaaatca cggcggcggc tttcttcaaa accaaatgcc
    39541 ttttgataaa attcgattga tgcttcaaga tctttcactc gaacacatgt gtgagccatt
    39601 ttcatagatt catctctcct tttcctttta ctcaatcata acaaaaagaa gtaaggaaat
    39661 agttgatttg tgcttgttca tgagaaattt ctcatgtaaa atggatagaa aataaaactg
    39721 ttaaggtgga aactattcat ggataaacca atttttggtg aaaaaaagca atttgctgat
    39781 tatcaaaccc gttatgcagc atatattgtc atagaaagag atcagcagat tgcagtaatc
    39841 gaggctccaa atggtgcttt ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa
    39901 gaagaagcga ttgtccgtga gctgatagaa gaaatgggaa ttgtggcaga aatttcttct
    39961 tatctgggag aagctgatga gtatttttac tcgaattatc gtgcaactta ttattataat
    40021 ccaggatatt tctttgttgc tgattcttgg aagcgtatag gagaaccaac tgaaaagacc
    40081 aataaaattt ggtgggtgag ccctcaagaa gcgcttgtga aattgaaacg tggtagccac
    40141 cgatgggcag tcgagagatg gatcgaaaaa aataaaaact gaaagaaagc gagtagatca
    40201 gctttctctc agttttttta tttattacag tacatatttt ttgattgctt cagctacacc
    40261 gtgctcgtcg tttgatgaag tgatgacatt tgctgcttct ttgacaagag aaacggcatt
    40321 tcccattgct actcctagac cagcatattc aatcatggat aagtcgttct cgttatctcc
    40381 gatagccatg atttcatctt gatcaatacc aaggtgctgt gcgagattcg caactgccgc
    40441 acctttactt gcttctttat ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt
    40501 gtatttatca cggaaagttt gcggtaaacg cgcaattgct gcatctaaaa tttctggttc
    40561 gtcgatcatc atcattttga cgatgttcat gtctggtgtc atttcttcag gtgttcgata
    40621 ttttagcggc atcttgacta aacttgcttc gtggatcgta taagcgctga tatctcgatt
    40681 aggcgtgtag atcgctttgt catcgatcga atggagatga ctgccgacac ggcgtgccat
    40741 tacttcgatc tctaggaaat cttcatgtgt cagtccgtga cgggcgatga ttttacctga
    40801 tttcgtttgt tgtacgagtg ccccgttata agtaataacg taatcattat cttgaaaaag
    40861 gttcaactcg tttaactgtt cctggacacc tggtaaggga cggccagtgc ataaaacgat
    40921 cgaaacacct tttgcaacag cagcggcaat cgcttctttt acttcttttg ttacttctct
    40981 tttctcattt aataaagtac catcgatgtc gatagcgact agtttgatag acataatttc
    41041 ctccaatata ttttaattta tttcaatcaa tgcgccatta cggatatggc tggcaaattc
    41101 ttgataagtt tcgtcaaata aatcataatg atcacgcaag gacggatcaa ccatttcttt
    41161 tggaaagaaa aatcgctcat ctccttgttc ttgtcccgct aatgcagcaa caagctgact
    41221 aacctgtgat aattctatca aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg
    41281 tctgccttgt tctgggcgat aaaaatcata aggtagatcg tagctagaat tcacagcagt
    41341 gtaataaaca ggattatatc cgaccttttc tactaacaaa gtgagttctt ggactaattt
    41401 tgaatcccgg ttgccagaaa aagtagcaga tttcagtggt ttacgattca aaaaacgttt
    41461 agctaaatca tttaaaatag gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa
    41521 gaccccatca tcaagtttga ggtattcttc taaagtaaag cttccgtcaa gaaatggcag
    41581 aagtaattga gaatggagtt caaaactgct ggcatcttct tgatacaact cgtgtgcgcg
    41641 atgaagcaga tgctccaaaa ttacttccat accacgggaa acaggatgga agtacacttg
    41701 cacatacatt tgatatcggc taacgatata atcttccact gcatgcatgc cactcatgga
    41761 aaaagcgatc ccgccttcat aaggacgaat cacacgtaat attcgggtaa gatcaaatgt
    41821 tccatattct gttccagtga aataagcatc ccgaagcaaa tagtccattc gatctgcatc
    41881 gatctgacta gaaatcattt ggacaacttg tgggttgggg taagtttttt ggatcacact
    41941 agctaccttt tcaggaaaac cttcttctac tcggttaaga atctgataca cttctgtttc
    42001 tggagaagtg atgatctcta cagtgatggc ttcatgattc gtatggaaga tatgttcaaa
    42061 tgtatgagag taaggaccat gaccgacatc atgcaacagt gccgcacata aagtcaccag
    42121 ccgttcctga tcatcccagc ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa
    42181 gatatcgcag attcgtcttg aaatctcata aacgcctaat gaatgagtaa aacggctatg
    42241 ttctgctccg tgaaaagtga aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg
    42301 gacttctttc gaattgatca aatctaagat tacttgatgc tggacatgga tatagttatg
    42361 gactggatca cgaaaaactt tttccatagg cagcatttga tatttataag gggttgtcat
    42421 aatgtagctc ctttatgtta ttacgttgtt ccattctctg gatttggttg ttccattgct
    42481 ccagctggat ttcttttttt agccagtcgg tagaatcctg taaatcactt gcagaataca
    42541 gcttttcaaa agcttttgca aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa
    42601 gcagttcttc aagtgttgcc attgtagcag gatccactgc aggatagcca tttgtaccaa
    42661 agtcttcttt taaagcagat tgataaaatc gcttcatgac ttgcccacgt ttttcctgat
    42721 taccgttgac acttaagtac atcataacag aaatcccatt tttgatccga cgctgagcga
    42781 taccggcaaa ttttttgcca tggatgctta aatcataagt tccaggacaa taagaatggg
    42841 tgatttcgaa tgcttgtatc ggtgcctttg gaaaagcgta tttagtcaat aggagcattt
    42901 gctcgtaagc gtcatctatg gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa
    42961 tattcaaaat tccttcatct gatacgatcc ctaatccacc tgaatttctg atcactggag
    43021 cgtaacctgc atcttccagt gtttggatcc cgtcacttag aaaaggagtt cgaacatcct
    43081 tcatacctaa gatgactgtg cagtcatatt gccagaaatg taaaaagatt tgctggtgtt
    43141 ctttagaata ttccgttaat atatctgtta atgcaaaagg ggaaaagtcg tttttttgat
    43201 aaatgtgctg atctagtcga atcgcagggt tcattcaacc ggctcctttg tataaattca
    43261 tattttctac aatattatac tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt
    43321 aaaaatggac aagttggttg acaaaacaaa agaaactagg aacaatgtag ctatgagaag
    43381 taaaggagaa tgaataatgg aattatcaca aggaacaccg atttcaatca aattgcgtac
    43441 aaaagtaaaa cagcaagggg aagtgcaaga tttttatttt gatttgaaag ggcaaatggt
    43501 gacaattggc gatacgctgt acatacgtta taaagagatt caggaggaga cgggcgatga
    43561 gattcctgta acgatcaaat tagttccaga tggtcatgta cagctgatcc gtgcaggaga
    43621 aatgcggatg cgtttgaaat ttggctataa agaacggtta gaaacatcct atcgaacacc
    43681 atacggtatg atccagattg ctacttttac gaaagaactg catgttagtt tgaaagaccg
    43741 tcccacagca gggaaggttc gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg
    43801 cgaatattat ttgaccttgg attttacagc gtaaatataa aagaaaagtt tgattttttg
    43861 tgtctgattt tgtatgataa ggagtagact gtgaaaggac gtgtcctgat tggaaattag
    43921 tgtatttgaa ggtgcaaaca aaagcgaatt atcaatgatc gaagtagcac acgcgatctt
    43981 agaacaacgt ggcgatgtca tggatttttc tgatttagct aaccagatcc aaaactatct
    44041 tgggaaatca gacagtgaaa ttcgtgattc actcgcacaa ttttatactg acttgaatat
    44101 tgacggcagc tttatctcat taggggataa tcgttggggc ttgcgttcat ggtatgctat
    44161 cgattcgatc gatgaagaag taaaccatgg aatggacgaa gaggatgaag atactccacg
    44221 tcgccgtaaa cgcaagaaag tcaacgcctt tatcaatgat gatgaagacg cgatcgatta
    44281 caatgacgat gacccagaag atacagatct gacagaagaa gatgacgatg atctgtttga
    44341 tgacgatgat gatgaagatg aagaaatcgc tgcttataat tctgacttgc aagaaatcgg
    44401 agcagatgac ccgacagatg atgaagaaga cttgccagga aatatcgaag aagatttaag
    44461 tatcatagac gaagacgatg atgatgaaga ttatgaagag gaagaatttt ctgactctga
    44521 aaaagaataa acattatttt cttacgagag gttgtgacag agacgatcag cctatcagaa
    44581 taaggaggtg tgacacgatt tttgtcacac cttctttttt gtatataaaa atcccaagta
    44641 aatacataaa aaataaaaaa attgaaactt ttagcgcacc ttgtacgtca tattaagtga
    44701 ggcagaaagg agtggagaat gatttttact ggatataaga aaaaacggca gatcgaaaaa
    44761 taccgtcacc tgctgaatgg actcatcgaa actgattatc aaaaaatgtt ccgtatcacg
    44821 atgaattacg tacatgataa agaagaggcc ttagatgtga tgcaagatag ttttcataaa
    44881 gccctgaact attttgaaaa agaaaaagag atcgagcatt ttagtgcatg gttttatcgg
    44941 atattgatcc gcacggcatt agatagttgg cgcagacaaa aaaggacgcc tgtggactta
    45001 tttgaaattc aagatatccc ccaacagtta cctttagaaa cttccgttgc agagctccat
    45061 atgattttag ataaaataga cagtccggaa aaagagattc tgatcctcta cttttttgaa
    45121 ggatttcgtt tgaaagaaat tgcgactatc ttggatatga atgaaaacac agttaaaacg
    45181 aaaatgtacc gctcattgaa tgctttacgt caaatcctag aatgaaggag agaagagtgc
    45241 catgtattcc caaaaagaaa atttaaaaaa actaaagaaa acttataaag aacaagcaat
    45301 tcccaaaggg cttaaagagg agatccgcca gcgttttatt atcgaagagc aatcatttat
    45361 aaaagcaaag cggagaaaga gacgtctcca agggttcgga gtatctttgg ctatcgtaat
    45421 gattacaaca agtagccttc tttttaatag tcaagttcga agctttgcag aagatttacc
    45481 catcttagga tcagtcattg aattgatctt aggcgaacgc tttactgatc gatctgaaaa
    45541 aatcgatatt caggtaccga agatcaatac acaaaatgag aaagaaaata aaacaattca
    45601 tggattaaat caaaaatact ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga
    45661 atatggaaat tttgaaacgg atcattacca agttttggga gattatcaga aaatcgtaga
    45721 cgataaccgg tttctcgtga ttgaaaggca gatcacccaa acagcagcag atagccatgt
    45781 tgaaaaaaga tacgacacaa tcgacaagaa aaacagtgtc cagctctcac ttccactttt
    45841 attcaaagat gacacctatc tatctgtttt gacaaaagaa gtcaaacggc agatggccga
    45901 acaagtcaag gaagatccat ctaagtacta ttggactgag caagatattc aagaagggac
    45961 gatagaaaaa ccaacacttg ttacgcctac acgaagtttt tatttgaaca aagagcatca
    46021 attagttttg actttttctc aatacgaaat tgctcctggt tatatgggta caccagaatt
    46081 tgtcattcct aagtccgtga caaaaacgat tttggcatcg gaagattatt tggatcatta
    46141 gacgagaagt agttgcgact agcagtttca attttcaagt aagtacaagt aaataataag
    46201 aagagaaaaa gagaaagaag agatatagtg aaaagaaatt atttagttat aagtattatt
    46261 gctttatccg tgcttttgtt tttcgaaatt acttcggtgg cctccttgat caccgaagta
    46321 aaaggacaaa cacactcttc ggagcattcg tcacaatctt tcaaacaatc agaacaatca
    46381 accattaaaa gccaatcaac tgaaacagct aacgtcaaag aagccattca agaatcagcg
    46441 gaaaaggtac aaagtcagtc atcacaatca acacaagaca gtacaaaaac gagccgagat
    46501 ccggctgtca aaagcgtcgc aatcagcttt gatgatggac caggagcgac cacaacaccg
    46561 cagcttctgc gaatcttaaa agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa
    46621 aatacggcgc agcatcctga aatcgtgaag cagacagctg aagcagggca cgagattggc
    46681 aatcacacat acgatcatca gaatctggcc actctttctg cccagtcgat gacggaggaa
    46741 gtgacgaaag cagacactga gatcaaaaaa gtcactggca aaacccccac ttttgttcgt
    46801 ccgccctatg gttctgtcac gaatgtagga gcaacgatca tccagcgacc gatcatcgaa
    46861 tggtctgttg attcagaaga ttggaagaca agaaatcctg atttgatttt gcagaagatc
    46921 caagcaacag tatacgatgg ggcaattatt ttattccatg atatctatcc tgaaacgatt
    46981 cgtgccgttc ctcaagtgat cgattatctg aaagagcaag ggtatcgcat cacgacagtt
    47041 ggggatttat tagggcatcc aacggctgtc gagaactatt atggcagaaa cgatcatcgg
    47101 ccagttcagt aagtgagaca agtatcttta ataaataaca cattttaata agtagttgaa
    47161 cacataacta ccttacttgc aaaggcttaa caatatctat acaggtacaa caaaaaactg
    47221 tccaccaatt tt
//
LOCUS       DAPBCS010000019        46770 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPBCS010000019 DAPBCS010000000
VERSION     DAPBCS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 46770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.019"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            245..2410
                     /gene="acm"
                     /locus_tag="RD521_001251"
     CDS             245..2410
                     /gene="acm"
                     /locus_tag="RD521_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDT7770999.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2682..3167)
                     /locus_tag="RD521_001252"
     CDS             complement(2682..3167)
                     /locus_tag="RD521_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDT7771000.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3257..3602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3647..4288
                     /locus_tag="RD521_001253"
     CDS             3647..4288
                     /locus_tag="RD521_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7771001.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4307..4942
                     /locus_tag="RD521_001254"
     CDS             4307..4942
                     /locus_tag="RD521_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7771002.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4960..5775
                     /locus_tag="RD521_001255"
     CDS             4960..5775
                     /locus_tag="RD521_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7771003.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6094..7530
                     /locus_tag="RD521_001256"
     CDS             6094..7530
                     /locus_tag="RD521_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7771004.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7719..10166
                     /locus_tag="RD521_001257"
     CDS             7719..10166
                     /locus_tag="RD521_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7771005.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10396..11463
                     /gene="rlmN"
                     /locus_tag="RD521_001258"
     CDS             10396..11463
                     /gene="rlmN"
                     /locus_tag="RD521_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDT7771006.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11656..12759)
                     /locus_tag="RD521_001259"
     CDS             complement(11656..12759)
                     /locus_tag="RD521_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7771007.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13312..13860
                     /gene="aac(6')"
                     /locus_tag="RD521_001260"
     CDS             13312..13860
                     /gene="aac(6')"
                     /locus_tag="RD521_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDT7771008.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14405..16066
                     /locus_tag="RD521_001261"
     CDS             14405..16066
                     /locus_tag="RD521_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7771009.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16136..16483)
                     /locus_tag="RD521_001262"
     CDS             complement(16136..16483)
                     /locus_tag="RD521_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDT7771010.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16567..17508
                     /locus_tag="RD521_001263"
     CDS             16567..17508
                     /locus_tag="RD521_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771011.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17509..18552
                     /locus_tag="RD521_001264"
     CDS             17509..18552
                     /locus_tag="RD521_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771012.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18558..19628
                     /locus_tag="RD521_001265"
     CDS             18558..19628
                     /locus_tag="RD521_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771013.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19618..20571
                     /locus_tag="RD521_001266"
     CDS             19618..20571
                     /locus_tag="RD521_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7771014.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20583..21593
                     /locus_tag="RD521_001267"
     CDS             20583..21593
                     /locus_tag="RD521_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7771015.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(21878..22699)
                     /locus_tag="RD521_001268"
     CDS             complement(21878..22699)
                     /locus_tag="RD521_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDT7771016.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22796..23074)
                     /locus_tag="RD521_001269"
     CDS             complement(22796..23074)
                     /locus_tag="RD521_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771017.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23342..25351
                     /gene="metG"
                     /locus_tag="RD521_001270"
     CDS             23342..25351
                     /gene="metG"
                     /locus_tag="RD521_001270"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDT7771018.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25830..26417)
                     /locus_tag="RD521_001271"
     CDS             complement(25830..26417)
                     /locus_tag="RD521_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771019.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26592..27914
                     /locus_tag="RD521_001272"
     CDS             26592..27914
                     /locus_tag="RD521_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7771020.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28069..28845
                     /locus_tag="RD521_001273"
     CDS             28069..28845
                     /locus_tag="RD521_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDT7771021.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            28838..29407
                     /gene="rnmV"
                     /locus_tag="RD521_001274"
     CDS             28838..29407
                     /gene="rnmV"
                     /locus_tag="RD521_001274"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDT7771022.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29400..30284
                     /gene="rsmA"
                     /locus_tag="RD521_001275"
     CDS             29400..30284
                     /gene="rsmA"
                     /locus_tag="RD521_001275"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDT7771023.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30735..31364
                     /locus_tag="RD521_001276"
     CDS             30735..31364
                     /locus_tag="RD521_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771024.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31488..32597)
                     /gene="ugpC"
                     /locus_tag="RD521_001277"
     CDS             complement(31488..32597)
                     /gene="ugpC"
                     /locus_tag="RD521_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDT7771025.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32806..33228
                     /gene="mgsA"
                     /locus_tag="RD521_001278"
     CDS             32806..33228
                     /gene="mgsA"
                     /locus_tag="RD521_001278"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDT7771026.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33551..34087
                     /locus_tag="RD521_001279"
     CDS             33551..34087
                     /locus_tag="RD521_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDT7771027.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(34127..35890)
                     /locus_tag="RD521_001280"
     CDS             complement(34127..35890)
                     /locus_tag="RD521_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771028.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(35893..37608)
                     /locus_tag="RD521_001281"
     CDS             complement(35893..37608)
                     /locus_tag="RD521_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771029.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38387..38611)
                     /locus_tag="RD521_001282"
     CDS             complement(38387..38611)
                     /locus_tag="RD521_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771030.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38802..41579
                     /locus_tag="RD521_001283"
     CDS             38802..41579
                     /locus_tag="RD521_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7771031.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41660..42079
                     /locus_tag="RD521_001284"
     CDS             41660..42079
                     /locus_tag="RD521_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDT7771032.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            42091..42561
                     /locus_tag="RD521_001285"
     CDS             42091..42561
                     /locus_tag="RD521_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7771033.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42578..43357
                     /locus_tag="RD521_001286"
     CDS             42578..43357
                     /locus_tag="RD521_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7771034.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43344..44165
                     /locus_tag="RD521_001287"
     CDS             43344..44165
                     /locus_tag="RD521_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7771035.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44182..45231
                     /locus_tag="RD521_001288"
     CDS             44182..45231
                     /locus_tag="RD521_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7771036.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45244..46248
                     /locus_tag="RD521_001289"
     CDS             45244..46248
                     /locus_tag="RD521_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7771037.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT        15486 a         8131 c         9627 g        13526 t
ORIGIN      
        1 atagattgaa aaaatgaaag caataagtaa aataaaatga atatatgcga aaaaataatt
       61 tacattagga aaaaccttgt aaaaacagtt ttttggcata tttatttttt ataaatattt
      121 gtaaaataac tatcaatttt ttagttaaaa gattggaaat gagtggggga ggaattgaag
      181 aaaggatttt tgagagatga tatagtaggt tttattttta gttaaaatag gggagataga
      241 aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta ctaatataac
      301 atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca gtaatgtcac
      361 ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag taaaattctc
      421 gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca attggccaag
      481 tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga ttgaaaataa
      541 aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat
      601 taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca gaaacgtaac
      661 cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata aaacagctac
      721 tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt ataaaacagg
      781 agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta acaataacgg
      841 cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac aaagattaga
      901 tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt atagaggaga
      961 agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt tttcagtaac
     1021 tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga aaataatggt
     1081 gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc attcggaagc
     1141 ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc atacagttaa
     1201 aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga agattttcaa
     1261 atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa gacgagcaga
     1321 tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac agggagaagc
     1381 tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag cacccaattg
     1441 gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg aaaatgatac
     1501 agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca cagcaactaa
     1561 aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat tcagagcaag
     1621 tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca atggaataac
     1681 ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat atacgtttaa
     1741 agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc aaaaaattga
     1801 aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag gacaaaaaac
     1861 atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag taaatctatt
     1921 agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg
     1981 tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg taacggaaga
     2041 caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca gttatacacc
     2101 ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa attttccaga
     2161 gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa tatttgctca
     2221 tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg tacctaaaca
     2281 gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat ctagttttgc
     2341 tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac aaaaatataa
     2401 aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa aagaataaaa
     2461 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
     2521 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
     2581 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
     2641 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
     2701 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
     2761 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
     2821 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
     2881 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
     2941 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
     3001 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
     3061 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
     3121 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
     3181 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
     3241 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
     3301 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
     3361 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
     3421 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     3481 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     3541 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     3601 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     3661 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     3721 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     3781 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     3841 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     3901 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     3961 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     4021 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     4081 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     4141 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     4201 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     4261 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     4321 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     4381 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     4441 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     4501 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     4561 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     4621 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     4681 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     4741 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     4801 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     4861 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     4921 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     4981 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     5041 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     5101 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     5161 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     5221 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     5281 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     5341 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     5401 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     5461 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     5521 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     5581 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     5641 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     5701 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     5761 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     5821 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     5881 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     5941 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     6001 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     6061 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     6121 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     6181 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     6241 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     6301 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     6361 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     6421 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     6481 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     6541 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     6601 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     6661 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     6721 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     6781 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     6841 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     6901 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     6961 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     7021 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     7081 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     7141 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     7201 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     7261 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     7321 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     7381 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     7441 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     7501 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     7561 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     7621 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     7681 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     7741 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     7801 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     7861 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     7921 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     7981 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     8041 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     8101 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     8161 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     8221 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     8281 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     8341 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     8401 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     8461 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     8521 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     8581 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     8641 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     8701 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     8761 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     8821 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     8881 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     8941 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     9001 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     9061 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     9121 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     9181 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     9241 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     9301 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     9361 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     9421 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     9481 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     9541 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     9601 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     9661 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     9721 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     9781 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     9841 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     9901 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     9961 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
    10021 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
    10081 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
    10141 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
    10201 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
    10261 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
    10321 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
    10381 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
    10441 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
    10501 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
    10561 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
    10621 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
    10681 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
    10741 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
    10801 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
    10861 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
    10921 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
    10981 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
    11041 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
    11101 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
    11161 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
    11221 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
    11281 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
    11341 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
    11401 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
    11461 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
    11521 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
    11581 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
    11641 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
    11701 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
    11761 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
    11821 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
    11881 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
    11941 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
    12001 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
    12061 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
    12121 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
    12181 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
    12241 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
    12301 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
    12361 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
    12421 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    12481 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    12541 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    12601 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    12661 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    12721 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    12781 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    12841 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    12901 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    12961 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    13021 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    13081 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    13141 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    13201 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    13261 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    13321 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    13381 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    13441 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    13501 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    13561 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    13621 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    13681 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    13741 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    13801 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    13861 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    13921 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    13981 gtgtaccaga ggttgtgaaa aagtcgctag tttcgagtag taagaaaaat tttgtgaaaa
    14041 tggctttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaacacc
    14101 gtttattcag ataagggact gtgacaagac ttttgtcaca gtttcttttt gtttattctt
    14161 ttttatcttt gtatggcaat aatagacaat ataacgaaaa ttatttaatg gcttttggaa
    14221 gaccatggaa aaccttgtac aaagctattt tttctgaaaa attccttgaa acctcacgaa
    14281 aaaatcatta ttttatgaaa aagacaaaaa aaagtgttga ttttttaata attattcatt
    14341 aaactcttaa acaacttcag aaaatattaa ttattctgaa aattccaata aaggggtacg
    14401 taaaatgaaa aagaaattta catttggttt ggttgcggta tgcggccttg tacttgctgg
    14461 atgttatggc ggcagttcag ccacaagcag tagttcagca aaaagcggtt cagcagatgg
    14521 aggaggcgta tttaacttag tcgtgcctca agaaatgcct acagctgact tatcagttgc
    14581 aacagataca atcagtttta cagcattgaa taacgtttat gaaggaattt accgtcttga
    14641 tgaagacagc aaaccacagc cggcaggagc tagcgagttg gcagaagtca gtgaagacgg
    14701 attaacttac aaaatcaaat tacgagaaga cgcaaaatgg tcaaatggtg atcctgttac
    14761 agcagcggat tacgtttatg gctggcaacg aacagtagac ccagctacag cttctgaata
    14821 tgcttacctg tatgcgccag ttgaaaatgc agaagacatt acagctggaa agaaagataa
    14881 atcagaacta gggatcaaag cagttggtga ctatgaatta gaaatcaaat tgacgaaaca
    14941 aacaccatat ttccaatatc tgcttgcttt cccatcattt ttcccacaaa aacaatccgt
    15001 agtggaagaa aatggggatg cctatgcatc tgcaagtgat aaagctgttt ataatggacc
    15061 attcacatta gaagattttg atggaccagg aacagataca gaatggactt ataagaaaaa
    15121 tgatcattac tgggacaaag atacagtaaa actttctgaa atcaaagtca gtgtagtaaa
    15181 agaatcttct actgcattga acttattcaa agatggccaa gcagatgacg tgatcttgtc
    15241 tggtgagtta gcacagcaaa acgcaaacga tccagcttat acttctgtta aagaagcgcg
    15301 gacaagctat atcgaattca atcaacgtga aaagaattca ccattcaaca atgtgaacct
    15361 aagaaaagct atttcttatt ctatcgatcg tgaagcttta gtaaaacaag tactaggtga
    15421 cggatcagtt gtctctacag gattgatccc atcagatatg gtaaaaagtc caacaacgaa
    15481 cgaagacttt gcaaaagaag ctggaaaatt agtttcttat gacaaagata aagcaaaaga
    15541 atattgggaa aaagcgaaaa aagaattggg cgttgattct ttagaatttg atttgatggc
    15601 ttctgatgat gattcatcga aaaaagtaat cgaatatgtt caaaactcaa tccaagaaaa
    15661 cttggatggc gtaacagtca aaccaacacc tgttccattc tctgtacgtc ttgaccgctc
    15721 aacttcgggt gattttgacg cagtactagg cggatgggct gctgactatg cagacccaag
    15781 cagctttact gatttgtttg tcacaggtaa ttcatacaac cgtggtcgct ggagcaatgc
    15841 agaatatgac gaagcagtta aagcagcttc atcaacagac gctggcgatg aacaagcccg
    15901 ttgggatgat ttccaaaaag caatcaagat catctctgat gatatgggtg tcgttccggt
    15961 gtaccaaaaa gcagaaggtc acctagtgaa tgaaaaaatc aaaggtatcg ttcaccatgc
    16021 ggcaggcgct tcatgggatt acaaatggac atacgttgaa gaataatcgt taataccgtg
    16081 agcatgagaa aaaacgaggc tgcggcaaat gtcgcaacct cgttctcata ttcacttatc
    16141 ctaacaaaga aaaaagcatg aaaatagcag aaagagcaat tagtattccg gcaatgatca
    16201 gccaaaagct gtacataccg tagctggcag acgagagcga ggaaaattct ggttttttct
    16261 ttcggttcct tgttcgtgca agatggaccg atcgctgaaa atgatcgaag aaacccgaag
    16321 aaaagaccca taaaaaaccg ccgataatca gaaacggcaa tgaccataag aatagatcat
    16381 tagataattg aatgagtgta aaggaatggt tgatgagatt ttttattaaa ataaccagaa
    16441 tcagtagaaa agctactgcg taggggatag attttttttt catttgattg ctcctttatt
    16501 tttggttcat tatctaataa tgtagcgaaa catctgtcag aagtcaaaag ctagggggaa
    16561 acattagtga atagttatat caaatatgta ttaaaacgtg tctttttcat gatcatcacg
    16621 ctttggttaa ttgcaacaat cacctttttc ttgatgcaat tgctgccggg cacgccatat
    16681 acgaatcagg aacgtttgag tcctgaaaca atcgagatgc taaataaaca agtcggtttg
    16741 gataaaccag ttatcgtaca atatgggatt tatctttcta atttgcttca aggagatttt
    16801 ggaatttcct tccaatttaa aaaccagcca gtcgcacatt tgttagctgg aagaatcgga
    16861 ccatccttgc agttagggtt acaggcaatt atctttggaa cagtcctagg gacgatttta
    16921 ggaacgatct ctgccatgaa gcaaaataca tgggcagata catcttctac actcgtagcg
    16981 atattaggtc gttccattcc aaactttgta tttgctgttt tattacaata tatctttgca
    17041 atcaaattac aagttttgcc aatcgcaaaa tgggatggct ttgtgtacac aatcttacca
    17101 acaattgcgt tggccatgtc gccgcttgct gactctgctc ggtttatccg tacggaaatg
    17161 gtggaagtac tgcatagtga ttatgtggaa ttagcaaaag caaaaggatt gagccgttgg
    17221 gaaatcgcct ttaaacatgg tcttcgaaat agtttgattc cattgatgac tttattagga
    17281 ccgttagctg tagcattgat gaccggatca ctggtggtag aaaatatctt tgctattcct
    17341 ggtatcgggg aacaattcgt taagtcaatc acaacaaatg attatcctac gatcatggct
    17401 gtaacgatcc tttactcatt tatgctgatt tttgttattt tgatcgtgga tctattgtac
    17461 ggtctagttg atccaagaat tcgtgtttca gaagggagcc gaggataaat ggaaatgata
    17521 ccaaagcgtt atgaaacagt tgcagatatc cctgcggatg aatttcttcc attacaaaaa
    17581 aataccgaag aagaacgtga acagattact gcaccttctt tgaattttat ccaagattca
    17641 tggcgccgtt taaagaaaaa caaagctgcc gttatttcaa tggttttact attggtcatc
    17701 attttgatct ctgtgatcac aatctttgtt tctcctcatg atccaactgc ccaaaacgtg
    17761 gcttatatca atttgcctcc tcgtattccg ggaatcaata tcgatggatt gaatggaaaa
    17821 gcaatggttg gtggtgaact tgttgataaa tatgctcaag caaacgtccc ttcaaacgta
    17881 aatttctttt taggaacaga cggtttggga cgtgatgtat taagccgctt atttatggga
    17941 atgagaatct cgttgctgat cgcatttatt gctgcattac tagatgtaac gattggtgta
    18001 gcttacgggt tggtttctgg tttgttaggc ggacgtgtcg ataacgtgat gcaacgtttt
    18061 ttggaaatct tatcaggaat tccaaacctc gttgtcatga ttttgatgtt ggtcgttttt
    18121 gaaccaggaa ttttttcaat cgtggctgct atggccatca caaactggat accaatggca
    18181 cgtatcgttc gtgcacaaac aatgaaatta aaagatcaag aatatgtttt agctggattg
    18241 acattaggtg aatcaagaat gaaaattgca ttcaaacata ttttgccgaa tatctctagt
    18301 gtcattatta ttcaaatgat gtttagtatt ccatctgcta ttttcttcga agccttttta
    18361 agctttatcg gtttaggtct tacgcctccg tcagcttcat taggaacgat gttgagcgaa
    18421 gggtacaaga cattcctgta tctgccttat ctattatgga ttccggcagt aactttatcg
    18481 gttatcatga ttggctttaa cttacttgct gatggattgc gtgatgcctt tgatcctaaa
    18541 atgaaagagt gaagacgatg acgaaaattt tagaagtgaa agacttagaa atctcttttg
    18601 atacgtatgc tggaaaggta cgagcgattc gcggtgtcaa ttttcatttg aataaaggag
    18661 aaacattggc gatcgttggg gaatctggaa gtggaaaatc agtgacgacc agaagcatca
    18721 tgcgcttgct gtcaagcaac gccaatatag atgctggtca aatcctcttt aaaggtaacg
    18781 atatcgttgg aaaatcagaa agagagatgc aaaaaatccg tggtaaagaa atagcgatga
    18841 ttttccaaga tccaatgacc tctttagatc cgacgatgcc cattgggaag caagtcgctg
    18901 agtcccttag aaagcataat aaagtttcaa aaaaagaggg acaaaaagct gcattagaat
    18961 tgcttaaatt agttggaata cccgaagctg agaaacgtat caacagttat ccgcatcagt
    19021 tctcaggagg tcagcgtcaa cggatcgtga tcgcgattgc actggtatgt tatccggaaa
    19081 tcctgattgc tgatgaacct acaacagcgc ttgatgtgac tattcaagca cagattttgg
    19141 agctgctgaa agaaattcag acaaaaatcg atacatcaat tatctttatc actcatgacc
    19201 ttggtgtagt agcgaatgtc gctgatcgtg tcgctgtcat gtatggagga agaatcgttg
    19261 aagttggaac atcggaagaa attttctaca acccgcaaca tccctatact tggggactgc
    19321 ttggttcaat gccgacatta gatagtgcga acgatcgatt gtatgctatt cctggttctc
    19381 caccggattt attgaacccg ccaaaaggag acgcattcta tccaagaaat gaattcgcga
    19441 tgaaaatcga tgcagaacaa gaaccaccat tttttgaact atcaaaaaca cacaaggcag
    19501 caacttggct acttgctccg caggcaccca aagtgacacc gccagaagaa atccaacgtc
    19561 gctgggctat ctttaaagag aaacaaaaac atacttacgg tcaaggagga attgctgatg
    19621 ccaaataaac tacttgaagt taaaggattg aaacagtatt tcaatgtagg aaaaaagaat
    19681 gaagtacatg ctgtaaatga catcagcttt cacatttatg aaggagaaac attcggttta
    19741 gttggagaat ctggaagcgg aaaatccact actggccgta cgatcattcg cttgaatgaa
    19801 cctactgatg gtgagatatt attcgacggt caagatataa cgaagatcaa agataaaaaa
    19861 ggtttgacga aattccggca tgatgttcag atgatctttc aagatccata cgcttcgttg
    19921 aatcctcgta tgaaagtcag agatatcatt gccgaaggaa ttgatgttaa tggactagct
    19981 aaatcatctg aagagcgtgc aaaaaaagtc gatgatctat tgagaactgt tggattgaat
    20041 ccaagccacg gtactcgtta tcctcatgaa ttttcaggtg ggcaacgcca gcgtattggt
    20101 attgcacgag cattggcagt aaaaccgcgt tttatcattt gtgatgaacc aatttctgca
    20161 ctagatgtgt cgatccaagc ccaagtggtc aatttgttgc aagatctcca aaaagaacat
    20221 caattgacct atctatttat tgcacatgat ttgtcgatgg ttaaacatat tagtgaccga
    20281 atcggtgtaa tgcataacgg cttgttatta gagatgggaa caagtgatga aatctataat
    20341 cacggtgttc atccttatac agaaagtttg ttatctgcta ttcctctacc agatccagac
    20401 catgagagaa aacgcagaag aatcaaatat caacctgaac cagatgacgg acaagtacgt
    20461 caacttcgtg aaatcgcacc agaacatttt gtctatgcaa ctgagcagga agtcccatat
    20521 tacgcaaaaa aactaaaacg acaaaaggaa tctcttctgg ttgctaacta ggagggacat
    20581 agatgctaaa agcttttaaa ttccgtatgt atccgacaga agaacaaaaa cagcaattga
    20641 ttcgtacttt tggatgtgct cgatttacct ataaccattt gctgaaaaag cggcagaaaa
    20701 gttggcaaca aacaggagta gccaattttt cgctcacacc cgctacatta aaaaaagaat
    20761 acccattctt aaaagaagta gacagtcttg ctttagcaaa tgcacagctt aatttggatc
    20821 gggcttttcg caattatttc aagggacgtg ccagcttccc aaagcttaaa acgaaaaaaa
    20881 gtatgtggca atcttatacg accaacaacc aaacccgtac gatctatctg gaaaatggct
    20941 acttaaagtt accaaaacaa aaagaactaa tcaagataaa tcagcatcgg ccagttgaag
    21001 gatcgattcg ctccgctacg atttctgctc gatataatga agaattttat gtcgcactac
    21061 tttgtgatgt ttcgccagta aaaaaagaat ctttagctaa atggatcggg atcgcctatc
    21121 atccgaagac attgatccaa acatctcgac ctctagaagt aactttacca aaatttcacc
    21181 agacagaaga aaagctgcaa catgcacagc ggaaattgaa tgtgaaagtc cgctccgctc
    21241 accatagaaa gatcaggtta gatcaggcaa gcaattatca gaaacaaaag agaaaagtaa
    21301 tggatcttta tctgaaacaa aagaaccagc gagaagatta tcttgaacaa ttatctggga
    21361 aattggtcaa acaatatgac tacttatttg tagaatcttt tccaaaagaa gaggcacatg
    21421 ctgatttttc tattcacgac tggcataaat taataacaaa attgcgttat aagtcccaat
    21481 ggtataataa aaaatttctt ttgatcaata ccgacggagc agaagaaagc aattccgtaa
    21541 gaaagagcca agtagtagaa aaaatgggca gacattcggt tatcaaggga taaaaaatga
    21601 atgaaactga taggagcgtt agggttagcc ttgttaataa aaagtcttaa tgacttttgt
    21661 ttctaggaat ttttcttttg aagaaaagct cactaattat gacaaaccaa taaaacaata
    21721 gcagaaaaag ttcgtacgag ctttttctgc tattgttaat tttttattca gaaagtttac
    21781 gaatacatca aaattctgaa acaaaaaacg taactcacaa agagttgttt ttgcgttttt
    21841 tcttgaatga ttaggtctga atcttttgct cacaacctta ttcatggtgc cccggcttga
    21901 gctgatagat ttttgaagga cggccgattc ctacaggacg ttcaccgatc tcatcgaaat
    21961 attggagcat agctttctta aaattggaat gatcgatcgt tttataatcg atccctaaga
    22021 atttagcaaa tacttttcga gcttccgtaa tcgtgaaatc tttacctaaa acttgtagaa
    22081 cttgcggctc gtgttccatt ttattgacta cacggttgaa tgctttcaag atgatttgac
    22141 tatggtcaaa agccaaagta tctttgccag aagactctcc agtgactaaa tcaagcgaga
    22201 tagccacttc tccgctagat aaatggatct tgttactatg acgttctagt gtgaaccagc
    22261 gtacttcctt ggcatcatct ccggcgatta gcggttcttc tccaataaag gctaagtagc
    22321 tgactgtgac gacccagcct cgcggatctc gattaggcgt actgaatgta tgcagttgct
    22381 caatattttc ttttgaaata accacaccag tttcttcctt tgtttctctt aagacgcttt
    22441 ctccagttga ctcatccttt tggacaaagc caccaggcaa tgcccatgaa tttcgataag
    22501 gatgcccttt tctttggatc aacaaaactt tcagctgatc ttcttctttg ttatagcaca
    22561 tcagtacgat atcgacagtc aaagagggct tgtcatattc tggacgttcc tgtttttgat
    22621 accagttaag aaattcttct tcacttgctt tatgttcata atataatttt tcttcttctt
    22681 tagaagcaaa acggttcatc tgaccacctc cattgacagt aaaagtactt tttaccttat
    22741 tgtagcagac tctgaaaaaa aaccaaggat ttacttgaaa aatctaggtt atcatttaaa
    22801 tatgaaaaat aaatggaagt atgagattga ataaaaagaa aagcagataa aacggcaata
    22861 ctttataccg ctcttcacga atggcaaaga agccacaaat aacgatatat acgtcaatga
    22921 taaaacgcaa aatccaagct gctgtcataa aactgttcaa atgcccatgg aggttaaaat
    22981 aaccatacat acgaatataa aaatagctga atattaaatg tgctaatgaa agaaaagcaa
    23041 tactaagtgt aaaaaaaaga tgtttcttct gcatgattgt tccagtccct tactaagata
    23101 gatagtcttt attataccac gtgtgagggg agaaataaaa agccgttttt tagtagatat
    23161 gaaacaaaac aaaaacccgc atttattcaa cctattcatc cttttaaagg atagattgaa
    23221 cgtagataga agtcgtttat ccagtctttt tcattttgaa aaaaagctaa cagcatgctt
    23281 tttttatggt atactagcaa ggaaacgaat tcgcatcatg cgataaagga ggaagagccc
    23341 cgtggcagaa aaggaaactt tttatatcac tacgccaatc tattatccaa gcggcaaact
    23401 tcatatcggc aactcgtata ctacgattgc atgtgacgca attgctcgtt ataaacggtt
    23461 gatgggcttt gatgtgtttt atctcactgg agtggatgaa catggtcaaa aaatcgaaac
    23521 aaaagcagaa gaactaggtg taaaaccgca agaatatgta gataaaatgg cggctgatgt
    23581 caagaaatta tggaaaacgc ttgatatcag ttatgataaa ttcattcgca cgacagatga
    23641 ttaccataaa gcagctgttc aaaaaatatt tgatcgttta ttagaacagg gagatattta
    23701 cttaggtgaa tatgaaggct ggtactctgt ttctgacgaa gaattcttta cagaaacaca
    23761 acttgcagaa gtgtacaaag atgacgaagg taacgtgatc ggtggtaaag cgccaagtgg
    23821 acacgaagta gaattggtaa aagaagaatc atacttcttc cgcatgagca aatacgcaga
    23881 ccgtctattg gaatactatg aagaacatcc cgaattcatc cagccagaat ctcgtaaaaa
    23941 cgaaatgatc aacaacttta tcaaaccagg attagaggat cttgcagttt cacgaacaac
    24001 ttttacttgg ggagtaccag tcaagagtga tccgaaacat gtggtgtatg tatggatcga
    24061 tgcattatcc aattatatta ctgcactagg atatggctcc gaagatgaaa gtctattcga
    24121 aaaatactgg cctgcagatg ttcagatggt cggtaaagaa atcgttcgct ttcatacgat
    24181 ctattggcca atcatgctga tggcccttga tttgcctcta cctaaaaaag tatttgggca
    24241 tggctggcta ttgatgaaag acggaaaaat gtctaaatca aaaggaaatg tcgtttatcc
    24301 tgaaatgctt gtggaacgtt atggtttaga tgcactccgt tattatttgc ttcgtgctgt
    24361 tccgtttggg tcagatggtg tctttacgcc agaagatttt gtttcccgtg tcaattatga
    24421 tctggcaaat gatctaggga acttattgaa tcgaacgatt gctatgatca acaaatattg
    24481 tgatggactc gttcctgact atgcctcact tgtcactcca tttgatagtg aactgtctac
    24541 gacggctgct aacgttgtcg gacgttacca tgatgcaatg gaaaaaatgg aattcaatac
    24601 agcattggct gaaatctggg tgctgatttc tcgtgcaaac aaatacattg atgaaacaga
    24661 accatgggta ttagcaaaag acgaagaaaa gaaagctgaa ttgaatagtg tcatgatcca
    24721 tttagctgaa tctttgcgta tcgcagcgat attacttcag ccaatcatga cagaaacgcc
    24781 agagaagatc ttcaatcaac taggattgga ctctgaaaca atgaatcttg aaggattaca
    24841 ctttggcgaa tttccttcag gaacaaaagt agttgcaaaa ggtacaccaa tcttcccacg
    24901 tttagatatg gaagaagaag ttgcttttat tcaagaaaaa atgtctgaag gtactcaaac
    24961 aaacgaagat acagtcaaat gggatccaga agaaacagaa cttgtttcaa cgaaagaaaa
    25021 acaaatcaaa tttgatgtat ttgaaaaagt agaactaaaa gtagcagaag tcatcaactg
    25081 tcaaaaagta gaaggggcag ataagttgct gcaattccga ctagatgctg gagacagcca
    25141 agaccgtcaa atcttgtcag gaatcgctga attttatcct gatccaagcg aattgatcgg
    25201 gaaaaaagtc gtcattgtag caaatctgaa accacgaaag atgcgtggac aaatcagtca
    25261 aggaatgatc ctttctgcag aagcaccaga tggctcttta caagtaatcg aagcaccaaa
    25321 atccatgcca aatggctcag aaatcgcata gaaattcaaa gggggtatga cactagttgt
    25381 gtcatacccc ctttttccga aatttgagaa gcaattttcg taaattcctt cttattactt
    25441 gaagccaatc atataataca gaaacagaga actatccaaa ataaatccta gaaaaagaag
    25501 tacgagcttc gctgattttt gttaatttag aataatacta ataggaatcg taaaaacaga
    25561 aaaaaagcat aatgataaag ggtcgggcag attttaattg attcatctgg tactcttttt
    25621 tattgtcctt acattcataa agtttgaatg caatgacttt attacatcta tatttatatc
    25681 taacagtagc gttgatgtgt taattataag ttctatcact ttatgttcaa ttagtttgtg
    25741 ctcttatatg tgtgattgaa aatatagata aaaaaacaat catcggcttt gacttcgatg
    25801 attgttttct ttattttgta aaaatcctat cacttgattt tatggtaacc ttttattttt
    25861 gtcttcaaac ggcttggtaa gagagcagta agcagtgcaa atgggatcga aaagacatac
    25921 cagaaaatcg tacgtccgat accataatgt tgatagagct ttgtcaacat cacgtgtaac
    25981 aagatcaacc ctaaaagcaa ggagccagct ataaaccaat gattatccca atgcatgact
    26041 tcactaagtg ccatcactcc aaaaggatag aaataaaaat agatcccgac taaggtcatc
    26101 cgccagttct ttgtaaatgt tccgatattc aataaaaaga aaatcgctga aagcaaaata
    26161 ccgataggcg gaaaaagccc aaccaaaaag gaatacaaca tcccccacaa gatcatcaca
    26221 ggacctttga tcaatgcaac gattgcgatg aacaaagtca gcaatattag tttttgtgat
    26281 aacgttgcaa cagtcattaa tccaaataaa attaatgtga accaatgcca atgggactgc
    26341 tgttgttctt tacgggaaaa atcgacccgg ttggataaat aatctgctaa tttcggataa
    26401 gaacgcttaa aatccataaa ctgttcctcc ttcgtggaaa aggtaggttt agtatacgaa
    26461 aaataaaaaa gaaatacatc cgtcttaaga gggagttttt cactgaaagt ggagaaaaaa
    26521 gaatggtaaa ttaaaatatt ctcagttata ctgaaaatta taccaaattt caaagaagaa
    26581 agaggatggg aatgaaaggc aaactaaaac gagaaatcaa tctgtttggc gcattagcta
    26641 ccgttatggg aaccgtgatc ggtgcaggcg tcttcttcaa aactgctgct gttactgcta
    26701 gtactcagtc tacaagtttg acattgctag cttggctttt gggtggtttc ttaacgatct
    26761 gtgctggatt gacagtggct gaattagcta cagcaatccc ggaaactggt ggagctgtaa
    26821 aatatattga agctgcgtat ggaaaattgc ccagcttttt attaggatgg gcgcaaagct
    26881 tgatttactt tcctgctaat attgctgcat tatcgattat ctttgcgaca caattgacga
    26941 acttattaca attatctacc gattatcttt tgttgattgc agtgatcacc gctgtttctg
    27001 tgacagggtt gaatttgtta gggacaaaag tcggtgcttc tgtacaatca gtgacgttga
    27061 ttgttaaatt aataccaatt gctgtgattg tcatatgggg attattgacc ccaggacaag
    27121 gaactgtcca gttatttcca atcgaagctg gaaaagatgt tacttttgtc gaaggattga
    27181 gcagtgcgtt gttagcaacg ttgtttgctt atgacggctg gttaggtgtc ggtgcaatgg
    27241 caggggaaat gaaacggccg gaaaaagatt tgcctaaagc aattattctt ggtttgagtt
    27301 ttgtaacagt cgtttatcta ttgatcaatt ttgttttttt gaaaactttg ccgattgatc
    27361 atcttgccgg taatttgaat gcagcttctg aagcatcaga cgtcattttt ggaggaatcg
    27421 gtggaaagct ggtcactatt gggatcttga tctcggtata cggtgcattg aacggttata
    27481 cactaactgg gatccgcgtg ccttatgcga tggctttaga agacgagcta cctttcagta
    27541 aacagctgac aaagctttca aagaagttta cggttcctta tgttcctgcg gttttccagt
    27601 tagcagtagc ttgtattatg atgagccttg gttctttcga ctttttaaca gacatgttga
    27661 tttttgtgat gtggctattc agtttactga tttttatcgg tgtctttatt ttaagaaaaa
    27721 aagcgccgga attgccacgt ccatataaag taccacttta tccaatcgtt ccgattattg
    27781 cgattctcgg tgctattttc attttaggta tgacgatgat gacgcaaacg aaattagcat
    27841 tgatcggtat aggtgttacc ttgataggta tcccagtcta ttatcaaaaa aagaaaaaac
    27901 gctcagaaga gtaaaatatt tcaacaacag acagaaaaag tacgaactag gtcctctaga
    27961 tcttcgtgct ttttctattt caataaaaca agttcttcta ttttgatttt tgctacttct
    28021 gcaagtagtg cttatgtgct aaagtaagaa tgaaaaggag agataaacat gatatttgat
    28081 tctcataccc atttaaatgc tgaacagttc aatgaagata ttccagaaac gattcagcga
    28141 gcacaagaaa tgggggtaac gaaaatggcg gtcgtgggct tcgatacgcc aacgattgaa
    28201 aagtctttgc aattaagcca tgactatcca aatatataca gtattatcgg ctggcatccg
    28261 acagaggctg gcagttatac gaaagaaatc gaaaataaat tacaagaaca attgacttta
    28321 ccaaaggttg tagcattagg ggaaattggc ttggattatc attggatgga agacccgaaa
    28381 gaagtccaag ataaagtatt tcgcagacag attgctatcg caaaagaaat gaacctacca
    28441 atcagtattc atactagaga agcacttgaa gacacgtatc aaatcctaaa agaagaagat
    28501 gttcgagata tcggtggaat catgcatagt ttcagtggtg attatgaatg ggcaaaacga
    28561 tttcttgact tagggatgca tatttcattt agcggtgtcg tcactttcaa aaaagcatta
    28621 gacgtccaag aggctgcagc aaatgtacca atcgaacgat tacttgtgga aacagatgcc
    28681 ccttatctgg caccagttcc ttttagagga aagcgaaatg aaccaggcta tactcgttat
    28741 acagtagaaa agattgctga acttcggaat cttccgacag aagaagtggc tgcccaaaca
    28801 tggacaaatg cgcaccgatt atttggtcta agcgaacatg gataagatga agatccaaga
    28861 gattatagtc gttgaaggaa aagacgatac aagaaggctg cgggaagtag ttgatgcaga
    28921 tacgatagaa acaatagggt ctgcaatcaa tgaagagatc ctgatgcaaa ttgaacatgc
    28981 ccaagagact cgtggagtga tcgtttttac cgatcctgat ttttcaggag aaaaaataag
    29041 aaaaatcatt atggaagctg ttcctcaggc aaaacatgct tttttgccgc gcacacaagc
    29101 taaaccaaag aaaaagggaa gccttggtgt cgagcatgca agtgatgctg ctattttaga
    29161 agcattgaaa aaggtcgtga cgccagacat ttcccaaacg gctgtttctg aaatcactag
    29221 acaagacctt ttagactatg gtttaatagc tggggcacaa gcaaaagaaa aacgagaaaa
    29281 actaggtgac gagttgcgca tcggttacac gaacggcaaa caattggaaa aacgtttgaa
    29341 gatgtttcgt attacgccga aagaactcgc ccaagcaatg aaaatagtgg aggaacaaca
    29401 tgagtgaata tcgtgaaatt gcgacaccct ctagaacaaa agagatttta ttgaaacatg
    29461 gtttttcatt taaaaaaagt ttaggacaaa attttctgac tgaaccaaat attttaagga
    29521 aaatcgtaga aactgcgcaa atagatgaac atacgaacgt gatcgaagtt ggaccaggaa
    29581 tcggtgcatt gacggaacaa ttagccaagc atgcaaaaca agtcgtagca tttgagatcg
    29641 atgatcgttt gatccctgta ttggctgata cgatgcaacc ctatgacaat gtgactattg
    29701 tacatcaaga tattttaaag accgatttgt caacagctgt tagagaaaca tttcatgagg
    29761 agcttccgtt aaaagtcgta gcgaatttac cttactatat tacgacaccg atcatgatgc
    29821 attttcttga atcagatttg gttgtggatg aactagtcgt catgatgcaa aaagaagtag
    29881 ctgatcgtat ctctgctgaa ccaggaacga aagcttatgg ttcattatcg attgctgttc
    29941 aatattatat ggaagccagt ttagcattca tcgtaccaaa aacagtattt gtccctcagc
    30001 caaatgtaga ttcagcgatt ttgaaactga caagaagaga tataccggca gtcgaagtga
    30061 cagacgaaaa agcatttttc cgattgacaa aagcggcatt tcagcaaaga agaaaaacgc
    30121 tttggaataa cttgcagcat tcgtatggca aagatgacca gacaaaagca tggttagcaa
    30181 aaagcttgga aacagctgga atcgatccaa aacgtcgtgg ggagactttg tcattacaag
    30241 aattcgccgc attaagcaat gcgatgtcag aaaatatgca gtaaaaaaag aggttgtgac
    30301 agaagttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
    30361 ctcatgtttt tcgtttgttt gggcttatta ccgcagatgc aagcttttga acaccgttta
    30421 agaaagaggt tgtgggcctg ccgtttaact ctaagtcaca aggaacaatt tcaaaaaaca
    30481 gcttctcatg tttttgagtt agttggtctt attacttgat gcaagctttt gaccactatt
    30541 atgccgaaca aggagatgtg acacaaattt tgtcacatct cctatttttg tttcttgatc
    30601 aaaaaattct tatacttgat actcacactc attttgtatg gatttagaaa agaatatgtg
    30661 tcttgacagt tattagtaag tgagttatac taagtcgaca tttatgtgtc aagacatatt
    30721 aggagagaga aaaaatgaga aaaaattcag ttcgaaaact aacgatttct gctttattga
    30781 ttgggatggg ggtcattatc ccaatggtca tgcctaaaat catgattcca ccagcttcct
    30841 ttactttggc gtctcatgtg ccattattta ttgcgatgtt ttttacacca ggagttgcag
    30901 tggctgttgc gctaggtacg acatttggtt tctttttaac aacgccggtt atcatcgcat
    30961 tgcgagcatt gtcacatctt gtgtttgcgt tgattggggc ttggtacttg caaaagcatc
    31021 cgagtatcgt tttaaaaaat ggacagttta cttttgctaa ttggcgtttc caagggttca
    31081 attttgtgat cggtgtgatt cattctttag cagaaatgct agtagtcagc atcttttact
    31141 ttattggtaa tatgcctgaa acatattatt ctcaaggata tttttataca gtattcatct
    31201 tgatggggat tggtgggctt atccacagtt tagttgatta taatattgct tattttatag
    31261 caggaacact cagcaaatat tttgatatcc ccgtatttac ggcagcaaaa aaagaaaatc
    31321 ggaaagagtc gattcaaggg tacgcgaaga aaacgacata ttagacatga tctctcaaaa
    31381 aaaaaactct catctagtaa tggataataa atcctctaag atgagagttt tttgtcctaa
    31441 aaagagctgc accaacatct tctggcacag ctctaacagg gggcaattta tggaagtttg
    31501 atgacttgtt ctgtttcttt atcaaagaag tggcctttgc tgatgttgaa ggcaaggtcg
    31561 actatttcgc ctggtttatg gaaatctctt gcatctactt ttgagatgaa ctcagtagaa
    31621 ccagtttttg tgtaaagcat cgtttcagct ccaagtaatt cggaaacgac aacttctgat
    31681 ttcactacgg cttcaggtga agcttcaagg gctacttgtt cactgtggat gtcttctgga
    31741 cgaataccga agataacttc ttttccttca taaccttttt caactaatac tttgtttttt
    31801 ccttcaggaa tacgaagctt aagcccatgt ccatctgtga taacaccatt tttcaatgtc
    31861 acattaaaga agttcatggc aggagatcca ataaagccgg caacgaacat attgacgggt
    31921 gtatcataaa cttctttcgg tgtaccgatt tgttggataa atccgtcttt catgataacg
    31981 attcggtcag ccatagtcat tgcttctgtc tgatcgtgag taacatagat ggtcgttgtt
    32041 tcaagacgct ggtgaagttt ggcaatctca gcacgcatgg ctacacgtaa tttagcatct
    32101 aaatttgata atggctcatc cattaagaag acttttgcat cccgaacgat tgcccgtccc
    32161 aatgccacac gttggcgttg tccaccagat aaagcagctg gttttcgttt caagtattct
    32221 gtcagaccta agatctctgc tgcattgtcc acacgttttt tgatgtctgc tttatcatat
    32281 ttacgtaatt tcaagccaaa agccatatta tcaaaaacag tcatatgagg atacaatgcg
    32341 tagttttgga aaaccatggc aatatcacgg tctttaggtg ccacgtcatt catgactttt
    32401 ccgccgatat ttagttctcc ttcagagata tcttccagtc ccgcgatcat tcgcaaagta
    32461 gttgactttc cacaaccgga aggaccaaca aaaacgataa attcacggtc tttgattgat
    32521 agattaaaat cagttacaga ataattttct gcattgtcat attttttata tacgtttttc
    32581 agagccattt ctaccataat acgttccaca ctttcttttt tagttttatt aactatttaa
    32641 agtataaatg aaagcgttaa ctaacttcaa tgtcatgttg aacaagaatt ttttcttttt
    32701 tttgtcaatg tgctagtttt aagctttaat aaatatccat aagaaataaa aactgataca
    32761 ctggagtaaa taagtatgtt aacaatatca aaaggagtgt ctcttatgaa aatcgctttg
    32821 atcgctcatg accgaaaaaa acctatgatg gtgaaattag caacagcgta taaaacaata
    32881 ttggaaaaac atgaactttt tgctaccggg acgactgggc agaggatcac ggaagcaacc
    32941 ggcttgccga tccatcggtt taaatccgga ccgttaggcg gggatcagca aattggagcc
    33001 atgatttcgg aggatcagtt agatttggtg atttttcttc gagacccgct ggctgcacag
    33061 ccccatgaac cagatgtaac agcacttatc cgtttatctg atgtctatga gattccgtta
    33121 gctacgaata tcggaacagc tgaggtgcta ctaagaggac tggaagccgg cttcctagat
    33181 tttcgaagaa tcgtccatga aatagacaag catccacttt cattttgatg gataagcgta
    33241 tggaaaaata acgtttaatg ttcgcctata tgtagctata aaagctaaac aaactgataa
    33301 tatgcgatat aatacgaacg tattggataa tataaacaat aatattagtt tttcgtattt
    33361 gcagtaaatg tgaaaatatg ctatagtttc tgtgggaaaa gatcccaaaa ataatacaga
    33421 gtagaaaata aagcgttaag tgctaagagg atgggatgtt gcctcttgga cgaaggacgt
    33481 aatgttcgcg gttttatttt cgcattcgct gcatgatacg atgtagcagc tcttaaagga
    33541 gatgctaaaa atgaagaaac accggttaga cgcacgtatg attgctatta tgggcttatt
    33601 gattgcattg atggtcactt tgtcccgact ggtagcaatc gagaccccat ttattaaaat
    33661 cagtgtcaca tttattccac aagtgatcat gggcattctt tttggtcctt tttggtcagg
    33721 gataggcgca gttttagctg atttagtagg aatggccctc ttctcgaaat ctgcattctt
    33781 tattggattt acattgaatg catttattga aggagcaatc tacggattct ttttctatcg
    33841 aaaagagatc acttggaaaa atgccattct ggctacgctt agtgtcacgc tgatcatcaa
    33901 cttatttttg acaccattat ggcttgcgtt gatgtaccat gtgccattgt tcagctgggt
    33961 cgtttgggcg ccccgcctgt taaaaacagt aatatggtta ccgattcaat caattgccat
    34021 ttattacgta ggacgtagta ttccttacaa aaaaatttta agaagtttag cgatccacgc
    34081 taaataaaaa acaagcagtc ttcccatccc agaagactgc ttgtttttat tgtgctgtga
    34141 attgtgcttg atagagccgt cggtaataac catctttttg aagcagctca tcatgggtgc
    34201 ccatttcgac aatagctccc tgatccatca ctaatatctg gtccgcattt ttgattgtgg
    34261 acaggcgatg ggcaatcaca aaactggttt ttccttccat cattgctaag aaggcttgtt
    34321 ggattttttt ctctgtcaat gtatcgactg agctggtagc ttcatctaag atcaacatag
    34381 ggggattact gatcatcgtt cgagcaatcg tcaacagttg gcgttgtcca tctgatatct
    34441 ttaacccttg accgccaatc ttagtatcta atttttccgg caaacgttct acaaattcat
    34501 acatataaga ggcttttagg gcatcattga tttctgcgtc acttgcttcc ggattcccaa
    34561 aacgaagatt ttctcgcaat gtactgtcaa atagccaagt atcttgtaac accattccaa
    34621 aacttttacg taatgtatca cgttggatag agcggatgtc atggtcatcg actgtaattg
    34681 ttccttttgt tgtgtcataa aaacgcatca gcaaattaac aagtgtggat tttccggccc
    34741 ctgtttttcc tactatagca atggtttgac ctggataagc agtaaaattg aaatcttcga
    34801 tcaaacgctg cttggggtca taagaaaaag aaacatgatc aaaagagact tttcctttga
    34861 tatttttggg ttccaaggca taggcatctg gcaaatcagg cttttcttct ggctgatcga
    34921 tcaaatcaaa tgctcgttct agaccagaaa aagcggtttg cagttgtgtc gtgattccag
    34981 aaagttcgat aaatggttta gaaaactgac ttgagtagat cgtaaagctg gaaatcacac
    35041 caacagtgat tgacgaacca ctcttcaatg ccaataggcc gccaactaaa ccaatcgata
    35101 aataagcaag gtgatctaca aaacgagaag acgggtttgt caaggaagaa gaaaattgtg
    35161 cccgttgtcc tttttcatat agatcagcat ttagcagttc aaattgctgt tgactagctt
    35221 cttcttgctg aaaagctttc acgattttct ggttaccgat tcgttcggtc acaaagcccg
    35281 agatatcccc aataatcctt tgctgtctat cgaaatttgt ttgtgaagca cgagcaacga
    35341 tccaactcac aaggaaaata attggtgtag aacaaagaac gaccaatgtc agaagtggac
    35401 ttagacgcat cataaaaata aacgaaagaa tgatgattgc cactccagaa aataattgat
    35461 tgaaggccgc agaaattgca atggaaatat tgtccatatc atttgtaaat cgactgacga
    35521 tatttccgtg ggagttttga tcataataac gaagtggcaa tcgattcaga tgagcaaatg
    35581 catcttttct taattttgca acggataagt aagctaaccg attgcttaac acttggatga
    35641 gccattgact gactacgttg attaaaacga taccaccgaa tagaataagt aaatggaaca
    35701 gtcgattgaa gcggacctgt ccttctccaa tcatttgatc gatactttga ccaatttgta
    35761 acgtcatcac aactgttgca atcccattga tgattcctag tatcacagct agccaaatgt
    35821 cttttgcata ggcagtaagg taaggaatga atcggcggag cgtctgcaag gataattttt
    35881 ttactttcat aactattctt cctcctgtga tgcgacgatt tcctgatatt ctgctgattg
    35941 agcaagtaat tcttcatggg taccctgagc agcaatctta ccttgatcca tcactaagat
    36001 atgatgtgct tcttgaatag agcggacacg ttgggagata ataatcactg tactgccaag
    36061 ataagtctgt agcgccttac gcaaatccaa gtctgtttga taatccaacg cacttaaaga
    36121 gtcatctaaa atcagtaatg ctggcttggc aatcaaagct cttgcaatcg tcagtcgctg
    36181 tttttgtccg ccagagaaat ttttcccatt ttccatgact tttgtatcta aaccatttgg
    36241 aagctgacgg acaaattctt ctgcctgagc gattttcaga gcttcccaac attctttttc
    36301 tgtggcgtct ttcttacccc attgaaggtt ttcacgaata gtcccggtaa atagtacaga
    36361 agtttgggga acaagagcaa tttgctgacg gagatgatcc agctgccaag aacgaacatc
    36421 cattccgtcc attataactg taccggaact gacatcatag aaacgcggaa tcaattgtgt
    36481 caaagtcgtt ttcccgcttc cggttggacc aatgattcca aggactgtat ttcgcttcag
    36541 ctgaaatgta atattttgga gagaaagacc agaatcagga gtataacgga agtcaacatg
    36601 gtcaaaagcg ataattgctt cagaagaaaa atcgggagta gtactgacct cagcttcaag
    36661 aatcgaattt tctgtgtcta gcacctcttt cacccgattt cctgaagctt cggcacgtgt
    36721 gaagatcacg acgaggtttg aaacaacgat cagtgccaaa agcatttgat tcatataatt
    36781 gatcaaagca agaacttgtc cttgttgcaa gctaccaata tttactttga tcccgccaac
    36841 tgtcagcaaa cagatgatcc cgacattcat gatcaaggtt gtagcaggtg ataatagggc
    36901 agagatattg gaaacacgta aataattgtt cgctagatca tttgtgcttt tatcgaactt
    36961 ctctgtttct ttttttgtct gcgcaaatgc gcggatgaca cgaacaccgc taagattctg
    37021 actaacgtgt tcattgagct gatctagttt ttcttgcact ttttggtaga gaggaacggt
    37081 ttttttgatg ataaaaaata aaatgattga gaaaataggc aataatgcta agaaaaacag
    37141 acctacttcc cagtcgatca caaaagccat gaccactgac ccgatactta aaaacggtgc
    37201 tctgatcaat aaacgaatga ccattgctaa agctagctga agctgattga tgtcgtttgt
    37261 cattcgggtg atcaaggtat ctgttccaaa atgattcaac tcgttgtgag agaaagtatt
    37321 gatttttttg attagctgat tacgtaactc tgtaccaaat ccttgtgaag ctactgaagc
    37381 atagtattgg caaatgatag cacaaatcag accaatcaca gacatagcga ccatccataa
    37441 gcccatttca atgatttttc ctcggtcatt catttttagt ccattatcga tcaaggaagc
    37501 catcattaaa ggcaggacaa gttcgaagac agcttcaaga aatttgaaga acgggcccaa
    37561 aatgatttgc ttacgatagt cttttgcata acgtaataac tgaatcatat gattcctcct
    37621 attatttatt tgtccacaat tgaaaaaaca agtactacca cattgtagct taggaaattt
    37681 agaatgaaaa gagaacaagc cttttcataa atgaaagttt tattatgaag aagctttaga
    37741 aattgaatag actatttttt ttaaaaaaaa gaaaatgaga aaacatgcca gtctcttcat
    37801 tcataggaac gtctatttta cttaattttt ctataaatga attctgcata aaaaatatat
    37861 caagtaaatg tttatagatg aaactctatt tacttctttt ttaatttgtt cttgcaatct
    37921 tttttgctac gtaatttttg taaaaaagtg ggttgagaga aatctaattg attttcaaag
    37981 aaggagtatg aaaatgagaa aaatatttat tcgtttattg tgggtactat cttagaggaa
    38041 taaataatag tggagaaaac tatggtttgg cgcagattct atcaggtttg atggaacgaa
    38101 gacaacctcc cgacatcaag tcaaaagcta cttgagattt tgatctgaaa ataaaaaaag
    38161 aaaacaaggt aggaacatca atgtttctac cttgttttcg taatcaatgg gaaaaaacta
    38221 agagtggtgt acgtaatcaa taaaaaggat ggtgacaaca gtacctcttt actctctgaa
    38281 agcatcataa ctcgtttttc ctcattatgc caataaattg ttgttatacc acatataagg
    38341 atttgtaata gctaaaaaca gacaggaaag ttgaaatctg tgaatcttat tttgtatgga
    38401 aggatttata gaacgcaaaa atcaagataa agatgaataa aaatcctgtt tcggctatat
    38461 attgcgtatc tacgccaata aaacgagttt gttctgtcat agccatataa agcaaaggca
    38521 aaaatacaac tacagcgaga taagatccga tgatttctcg aaaagttttc caggaagtat
    38581 gtttcctgtt tttatattta tccgatttca ttgaatagtc atagcctcct tattctttca
    38641 tatgttgaaa ttccagtata aaaactggaa ggaactgtac gttttttaga ataaaaatta
    38701 taagggataa gtaggctgct gtctataata agatatattt ttcaattctt tattaaagat
    38761 tttattgaca aaatgaatcc gttttctatg atggaggagg ggtgaataag ttgttgaaga
    38821 aggaaatttt atgttactta gagaaccaga cagccttttt cgatttagaa catatgaacg
    38881 aggtctttac agcacaaagg ttagctgatc attttggggt aaaaagaaac acagtcagtc
    38941 attatttgaa ccagttaaat gatgagcaac tactagtcaa aatcaattct cgaccagtga
    39001 tttatttcca caaagaagct tttgaaaaac aattttttaa gttacgcacg aatttttatt
    39061 tttctatcga tgatctcaaa gaggaacagc cttttttcgc gcaaccaaaa gacttattct
    39121 cttttatgat cggatacgac gccagtttga aagaaagtat cgaacaaatc aaaacagctc
    39181 tttactatcc agatggcgga ttaccacttt tgattacagg cgaaagtggc acaggtaaaa
    39241 gttatttagt taatttggtc tatcagtatt gtctgcttca tgatctgcta gaagattctg
    39301 caccattcat cacttttaat tgtgcacaat atgcagataa tcctgaactt ttgactagca
    39361 atttgtttgg acatgttaag ggagcgtaca ctggtgcaga agaaaataaa aaaggggctt
    39421 ttgaagcagc agatggtggg atcttgtttt tagacgaggt acatcgctta acaccagaag
    39481 gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat ttatcgtatg ggagaaccta
    39541 atttgtcacg ccggatcaaa acacgtctct tttttgctac gactgaagag attactagtc
    39601 atttcttaac aactttcatt cgaagaatac ctatccgaat cgaactacct gcacttaatc
    39661 agcgtagtcg taatgaacgg ctagaactga tttactcttt ttttctcgaa gaacaaagaa
    39721 aaatggggag ccctttgtca gtcagtggac cagctttgtc acttttaaca agtcagaaat
    39781 tatccggaaa cataggagag ctgaaaaata tcgtcaaagt ttcagcagca aaagcatatg
    39841 ctgaacagcg tgaacagcct gaaatccatg tcacgatcca tcatttgcaa aaagaactac
    39901 ttgcgcagcc tattactaaa aatacagcac agcaaggaat ctatattacg caagaccaga
    39961 ccttagagca attaatggag aagcaacagc ccgaacagca gcgaatcgtt aaaagttttg
    40021 agcaaatcct aattgatttc aagaaaaata actgtctgct atattcttgc gaaggaaaat
    40081 taaagcaaga agtgattcaa ttatttgatt ttttgctgtt tgagaccagt agacaagaaa
    40141 aacacgagct gctggtttat tggacacaat ctatccgaga gacctttcga caaatggaaa
    40201 ctgcctatca aatcaaattc gatggaaaca gtgtctatgc cttgagttac tatctctatc
    40261 agcgaagcac tatcaaatgg ttaccggaag aacatgatat tatccaacta atcaaagaat
    40321 tagaacaaca agtttctcag tcttatccat ctagttatca ctatgtccaa cgaattctgg
    40381 aattaagtaa gatcaaatta gatattgaag acacaagtat ggatcggatt ttactgacga
    40441 tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact gccaaaagcg atgattgctg
    40501 ctcacggtta tgcaactgct agcagtatgg caaatgtagt aaatcgttta ttacaagagg
    40561 atctatttga atcatttgat atgccactag atgtcacacc acagcagatt gcttcagaaa
    40621 tccttgatta ttgcgaaaac agcgatgttt caaatggatt gatcatatta gtagacatgg
    40681 gatctctgaa agaaatccat cagtttttta aaaaacagtt atccgtaccg cttttgattt
    40741 tgaataatgt gacgacacct ttagcaatca cagtagggga atgcctgcaa aaaaatgtat
    40801 cattagaaga gatagcagaa gagactgttc gacagatcca gccagaatgg cgactgctct
    40861 atccagagga aaacaaaccc aaagcactca ttactacttg cttcactgga atcggtacgg
    40921 cggtccactt aagtgaactg ctagaaaaaa gcttacctac ggcttgtcag ttgaaaatca
    40981 tcccatatga atatcagcaa ttaaaagata agaaaaacag tgacccatta ttttccatat
    41041 acgaagtcgt aggaatgatt ggcaccacag atcctgatat caccaatatc gactatcttt
    41101 cattagaaga cctcatctcg ggggaaaaga tgtctgtact agctgaatgg ttggacacta
    41161 ctatgaatgc ttcagagaag gaaatattca atcagcgcat cattcgtaat ttttcattgg
    41221 aaaaagtact ggattccgtg actattctag atacagagaa ggtgatggga gaaatcgatc
    41281 attttatgag agaattagag gtgcagttga atcgaagcat cagcaatccc aaaaaattag
    41341 cgttgtatgt ccatgtgagc tgtctcattg aacgattgat ccgacaaatg ccaatcgaaa
    41401 cctatcaagg attagagaaa tggaagcagt gtcaaaaaga agggttatct gctattaaat
    41461 ctgcgtttag tgtcattgaa gagcattata gtgtcatgat ccccaattct gagattgcct
    41521 atatctatga tattttgagt gaagatacag agtttctctc gatagaagaa gaattttgaa
    41581 cataagcaag ttgttgccac taattttggc atgcaacttg cttttttagt atgtgtaagt
    41641 gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg gaaaatttgc
    41701 ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta ttactgtatt
    41761 cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc tttttatgag
    41821 gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg tcaacaacga
    41881 attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga atttgccact
    41941 attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt tgatccagca
    42001 ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca atcagaaaat
    42061 agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt cgaccatcgg
    42121 ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga ctgtattttg
    42181 attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa attagccaag
    42241 ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc gttgaaaagc
    42301 ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga tgcatataag
    42361 ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa ggcaaaacct
    42421 ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga acgcttgaaa
    42481 cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga taaaaaagtg
    42541 gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag cagtcttatt
    42601 aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt tgctttctcg
    42661 tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa ctgggatcat
    42721 tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg cttcgattcc
    42781 acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag ctggagccgg
    42841 tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga tattgaaaaa
    42901 tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt atgcagaaga
    42961 aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc tttccttgat
    43021 gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg gaaatctatt
    43081 aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt tgattccagc
    43141 tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac catatttttt
    43201 cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag ctatttttgg
    43261 tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac cacttcaaac
    43321 aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc aaaataacga
    43381 aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct tggaattatg
    43441 aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa aagttatatc
    43501 agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt aacacgaccc
    43561 cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa aataaggatg
    43621 atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg ggacctcttg
    43681 cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca ggtgtgggta
    43741 cctctttagc catgcaagga aatattttag gccctatctt gttcattttg attttcaata
    43801 ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta ggaacgggtt
    43861 ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc gcgtctatta
    43921 ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat attcctttaa
    43981 aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat atcgttccaa
    44041 atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa gaagtgaaac
    44101 cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg atagggattt
    44161 tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga agaaaaggaa
    44221 gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat gaaaaaaaca
    44281 gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc agcgaagcca
    44341 gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta ttttttcaat
    44401 tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag cgggaaaagt
    44461 gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt tgttgctatc
    44521 acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga tttaggtgcc
    44581 ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt acagttacta
    44641 ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat agaacaagac
    44701 tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca aaaaacggaa
    44761 gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt caccattgga
    44821 tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga aacaatccgc
    44881 gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct ggaggcagat
    44941 gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg ttcgcaaaag
    45001 ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac aaaaaaagca
    45061 agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc tgtattagcg
    45121 cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg tattgacttg
    45181 aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata agaggtggaa
    45241 atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc actagcttta
    45301 cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga cgctgtctat
    45361 tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta tatgaatggg
    45421 aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac tggaaacaaa
    45481 agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac tcaagaagta
    45541 gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt tatcgataaa
    45601 gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc tcctggaaca
    45661 gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg agaattcgaa
    45721 gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt agtggaagca
    45781 gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg tgatagtatg
    45841 cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc catgtgttac
    45901 tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt tcttcacggg
    45961 acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga agatgaacag
    46021 agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa ttatacattg
    46081 atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg acgtgttttg
    46141 tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga gcagttgaac
    46201 tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga tagactggat
    46261 aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt ttttaaaata
    46321 ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca acttataatc
    46381 tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac tatttagttg
    46441 ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta catagcgttt
    46501 tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca gagacaatct
    46561 gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg tattttaaat
    46621 caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag taacattgtc
    46681 aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa aatgtagact
    46741 attgtaacat atcattgatt atttcaagta
//
LOCUS       DAPBCS010000020        44941 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPBCS010000020 DAPBCS010000000
VERSION     DAPBCS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.020"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            212..604
                     /locus_tag="RD521_001290"
     CDS             212..604
                     /locus_tag="RD521_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7771038.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="RD521_001291"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="RD521_001291"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDT7771039.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="RD521_001292"
     CDS             3165..4775
                     /locus_tag="RD521_001292"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDT7771040.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="RD521_001293"
     CDS             5067..5936
                     /locus_tag="RD521_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDT7771041.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="RD521_001294"
     CDS             6101..7372
                     /locus_tag="RD521_001294"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDT7771042.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLITTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="RD521_001295"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="RD521_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDT7771043.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="RD521_001296"
     CDS             8763..9026
                     /locus_tag="RD521_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDT7771044.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="RD521_001297"
     CDS             9471..9920
                     /locus_tag="RD521_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7771045.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="RD521_001298"
     CDS             9977..11395
                     /locus_tag="RD521_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7771046.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="RD521_001299"
     CDS             11395..12255
                     /locus_tag="RD521_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDT7771047.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="RD521_001300"
     CDS             12429..13202
                     /locus_tag="RD521_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7771048.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="RD521_001301"
     CDS             13349..13714
                     /locus_tag="RD521_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDT7771049.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="RD521_001302"
     CDS             complement(13862..15070)
                     /locus_tag="RD521_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7771050.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="RD521_001303"
     CDS             complement(15089..15367)
                     /locus_tag="RD521_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771051.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="RD521_001304"
     CDS             15521..16216
                     /locus_tag="RD521_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDT7771052.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="RD521_001305"
     CDS             complement(16658..17455)
                     /locus_tag="RD521_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDT7771053.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="RD521_001306"
     CDS             complement(17452..18636)
                     /locus_tag="RD521_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDT7771054.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="RD521_001307"
     CDS             complement(18661..19908)
                     /locus_tag="RD521_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDT7771055.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="RD521_001308"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="RD521_001308"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDT7771056.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="RD521_001309"
     CDS             21888..22202
                     /locus_tag="RD521_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDT7771057.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="RD521_001310"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="RD521_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDT7771058.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="RD521_001311"
     CDS             22887..23531
                     /locus_tag="RD521_001311"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDT7771059.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="RD521_001312"
     CDS             23546..23875
                     /locus_tag="RD521_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDT7771060.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="RD521_001313"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="RD521_001313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDT7771061.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="RD521_001314"
     CDS             24863..25687
                     /locus_tag="RD521_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDT7771062.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="RD521_001315"
     CDS             25680..26027
                     /locus_tag="RD521_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDT7771063.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="RD521_001316"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="RD521_001316"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDT7771064.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28180
                     /gene="dinB"
                     /locus_tag="RD521_001317"
     CDS             27077..28180
                     /gene="dinB"
                     /locus_tag="RD521_001317"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDT7771065.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPL"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD521_001318"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="RD521_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDT7771066.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD521_001319"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="RD521_001319"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDT7771067.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="RD521_001320"
     CDS             31657..32451
                     /locus_tag="RD521_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771068.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="RD521_001321"
     CDS             32441..34483
                     /locus_tag="RD521_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771069.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="RD521_001322"
     CDS             34764..34913
                     /locus_tag="RD521_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDT7771070.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="RD521_001323"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="RD521_001323"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDT7771071.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="RD521_001324"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="RD521_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDT7771072.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="RD521_001325"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="RD521_001325"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDT7771073.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="RD521_001326"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="RD521_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDT7771074.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="RD521_001327"
     CDS             39330..40412
                     /locus_tag="RD521_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDT7771075.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="RD521_001328"
     CDS             40412..41215
                     /locus_tag="RD521_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDT7771076.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="RD521_001329"
     CDS             41285..42025
                     /locus_tag="RD521_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7771077.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="RD521_001330"
     CDS             42036..43040
                     /locus_tag="RD521_001330"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDT7771078.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="RD521_001331"
     CDS             complement(43085..44929)
                     /locus_tag="RD521_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771079.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14452 a         7837 c         9349 g        13303 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat tactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt tgagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttga ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgccggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatga gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 t
//
LOCUS       DAPBCS010000021        43926 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPBCS010000021 DAPBCS010000000
VERSION     DAPBCS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.021"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(313..903)
                     /locus_tag="RD521_001332"
     CDS             complement(313..903)
                     /locus_tag="RD521_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDT7771080.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1426..1587
                     /locus_tag="RD521_001333"
     CDS             1426..1587
                     /locus_tag="RD521_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7771081.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1606..2100
                     /locus_tag="RD521_001334"
     CDS             1606..2100
                     /locus_tag="RD521_001334"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDT7771082.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2093..2254
                     /locus_tag="RD521_001335"
     CDS             2093..2254
                     /locus_tag="RD521_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771083.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2738..3761
                     /locus_tag="RD521_001336"
                     /pseudo
     CDS             2738..3761
                     /locus_tag="RD521_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4104..4360
                     /locus_tag="RD521_001337"
                     /pseudo
     CDS             4104..4360
                     /locus_tag="RD521_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4747..4974
                     /locus_tag="RD521_001338"
     CDS             4747..4974
                     /locus_tag="RD521_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDT7771084.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4990..6156
                     /locus_tag="RD521_001339"
     CDS             4990..6156
                     /locus_tag="RD521_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDT7771085.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6391..7434
                     /gene="hrcA"
                     /locus_tag="RD521_001340"
     CDS             6391..7434
                     /gene="hrcA"
                     /locus_tag="RD521_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDT7771086.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7528..8091
                     /gene="grpE"
                     /locus_tag="RD521_001341"
     CDS             7528..8091
                     /gene="grpE"
                     /locus_tag="RD521_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDT7771087.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8142..9971
                     /gene="dnaK"
                     /locus_tag="RD521_001342"
     CDS             8142..9971
                     /gene="dnaK"
                     /locus_tag="RD521_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDT7771088.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10122..11288
                     /gene="dnaJ"
                     /locus_tag="RD521_001343"
     CDS             10122..11288
                     /gene="dnaJ"
                     /locus_tag="RD521_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDT7771089.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11656..12393)
                     /locus_tag="RD521_001344"
     CDS             complement(11656..12393)
                     /locus_tag="RD521_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7771090.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(12395..13897)
                     /locus_tag="RD521_001345"
     CDS             complement(12395..13897)
                     /locus_tag="RD521_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDT7771091.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            14156..16150
                     /gene="uvrB"
                     /locus_tag="RD521_001346"
     CDS             14156..16150
                     /gene="uvrB"
                     /locus_tag="RD521_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDT7771092.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            16160..18976
                     /gene="uvrA"
                     /locus_tag="RD521_001347"
     CDS             16160..18976
                     /gene="uvrA"
                     /locus_tag="RD521_001347"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDT7771093.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            19239..20504
                     /locus_tag="RD521_001348"
     CDS             19239..20504
                     /locus_tag="RD521_001348"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDT7771094.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            20508..21239
                     /locus_tag="RD521_001349"
     CDS             20508..21239
                     /locus_tag="RD521_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDT7771095.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            21374..22258
                     /gene="rapZ"
                     /locus_tag="RD521_001350"
     CDS             21374..22258
                     /gene="rapZ"
                     /locus_tag="RD521_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDT7771096.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            22255..23253
                     /locus_tag="RD521_001351"
     CDS             22255..23253
                     /locus_tag="RD521_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDT7771097.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            23287..24222
                     /gene="whiA"
                     /locus_tag="RD521_001352"
     CDS             23287..24222
                     /gene="whiA"
                     /locus_tag="RD521_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDT7771098.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            24790..25446
                     /locus_tag="RD521_001353"
     CDS             24790..25446
                     /locus_tag="RD521_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771099.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            25625..26190
                     /locus_tag="RD521_001354"
                     /pseudo
     CDS             25625..26190
                     /locus_tag="RD521_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26889..27410)
                     /locus_tag="RD521_001355"
     CDS             complement(26889..27410)
                     /locus_tag="RD521_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDT7771100.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            27608..31288
                     /gene="nifJ"
                     /locus_tag="RD521_001356"
     CDS             27608..31288
                     /gene="nifJ"
                     /locus_tag="RD521_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDT7771101.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            31477..32364
                     /locus_tag="RD521_001357"
     CDS             31477..32364
                     /locus_tag="RD521_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDT7771102.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            32361..33530
                     /locus_tag="RD521_001358"
     CDS             32361..33530
                     /locus_tag="RD521_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771103.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            33554..34909
                     /locus_tag="RD521_001359"
     CDS             33554..34909
                     /locus_tag="RD521_001359"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDT7771104.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(34981..36786)
                     /gene="glmS"
                     /locus_tag="RD521_001360"
     CDS             complement(34981..36786)
                     /gene="glmS"
                     /locus_tag="RD521_001360"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDT7771105.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            37196..37486
                     /locus_tag="RD521_001361"
     CDS             37196..37486
                     /locus_tag="RD521_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771106.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            37499..38524
                     /gene="sppA"
                     /locus_tag="RD521_001362"
     CDS             37499..38524
                     /gene="sppA"
                     /locus_tag="RD521_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDT7771107.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            38527..39186
                     /locus_tag="RD521_001363"
     CDS             38527..39186
                     /locus_tag="RD521_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDT7771108.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            39343..40275
                     /locus_tag="RD521_001364"
     CDS             39343..40275
                     /locus_tag="RD521_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771109.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            40429..41718
                     /locus_tag="RD521_001365"
     CDS             40429..41718
                     /locus_tag="RD521_001365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDT7771110.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            41965..43215
                     /gene="clpX"
                     /locus_tag="RD521_001366"
     CDS             41965..43215
                     /gene="clpX"
                     /locus_tag="RD521_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDT7771111.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            43298..43885
                     /locus_tag="RD521_001367"
     CDS             43298..43885
                     /locus_tag="RD521_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDT7771112.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT        14628 a         7472 c         9424 g        12402 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt
     2641 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     2701 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     2761 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
     2821 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     2881 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     2941 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
     3001 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     3061 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     3121 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     3181 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
     3241 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     3301 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
     3361 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
     3421 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
     3481 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
     3541 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
     3601 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
     3661 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
     3721 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     3781 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     3841 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     3901 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     3961 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     4021 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     4081 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     4141 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     4201 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     4261 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     4321 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
     4381 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     4441 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
     4501 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     4561 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     4621 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     4681 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     4741 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     4801 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     4861 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     4921 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     4981 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     5041 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     5101 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     5161 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     5221 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     5281 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     5341 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     5401 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     5461 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     5521 gaaggatatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     5581 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     5641 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     5701 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     5761 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     5821 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     5881 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     5941 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     6001 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     6061 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     6121 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     6181 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     6241 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     6301 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     6361 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     6421 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     6481 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     6541 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     6601 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     6661 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     6721 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     6781 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     6841 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     6901 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     6961 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     7021 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     7081 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     7141 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     7201 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     7261 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     7321 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     7381 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     7441 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     7501 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     7561 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     7621 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     7681 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     7741 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     7801 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     7861 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     7921 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     7981 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     8041 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     8101 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     8161 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     8221 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     8281 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     8341 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     8401 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     8461 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     8521 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     8581 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     8641 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     8701 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     8761 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     8821 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     8881 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     8941 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     9001 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     9061 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     9121 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     9181 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     9241 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     9301 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     9361 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     9421 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     9481 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     9541 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     9601 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     9661 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     9721 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     9781 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     9841 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     9901 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     9961 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
    10021 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
    10081 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
    10141 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
    10201 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
    10261 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
    10321 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
    10381 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
    10441 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
    10501 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
    10561 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
    10621 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
    10681 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
    10741 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
    10801 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
    10861 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
    10921 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
    10981 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
    11041 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
    11101 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
    11161 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
    11221 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
    11281 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
    11341 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt
    11401 tttgtagttg tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat
    11461 agtttctgaa ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc
    11521 ttatgcgatg gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct
    11581 ggtacagtta agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcgaccggg
    11641 catttttggt ttttattaaa tatttaatac tttattcaag aaatcttttg tccgttcatt
    11701 ttgcggattt tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat
    11761 gaacatgaca cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat
    11821 cgtcatacct tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg
    11881 gtcgagtgca gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg
    11941 agcaatagcc acacgctgct tttgtccacc ggaaagcatt tcaggataca tatctttttt
    12001 atcttctaga ccaactgtct gcagcaattt gattgctttt ttctctgctt cttctctaga
    12061 ttctttcttc aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa
    12121 taggttaaag tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct
    12181 tttatccatc aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg
    12241 attcaagcaa cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac
    12301 atctccttcg ttgatggaca cattgatgtc gtttaatact gtgttatcgc cgtatttttt
    12361 gactaaatgc tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt
    12421 ttgctaattt tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa
    12481 catacgtact ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta
    12541 caccgattgc ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg
    12601 atgggatcat gatgcgtatc gcttgcggca aaatgatttt ttgcattgtc cgattataag
    12661 aaagacctaa acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc
    12721 gaacaatctc tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga
    12781 agtcaggaat cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa
    12841 ccatcattgg aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc
    12901 gtattggtgc tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg
    12961 agatcaaagt taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt
    13021 ggataagtcc aagaaatgtt gattcatcag cagtggaaga ctcactggaa gtttctgaac
    13081 catccgctac atattcttta ataatcttat cgtattcgcc tgttcttttt aattctttca
    13141 atccttcatt gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat
    13201 attttccgcc agattctttt ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat
    13261 aaccgattac cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt
    13321 acaattgatc agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc
    13381 ttaaaaattc agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt
    13441 cataagactt gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt
    13501 aagaatcaga aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca
    13561 ttccgtctgc ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca
    13621 ttcggatgtt aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt
    13681 cgtactcgcc ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta
    13741 cgtatacgtc ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg
    13801 ttgtagtgtt gtcagcttgt gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta
    13861 agaaaacaaa tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat
    13921 attttattat tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga
    13981 aaaaaatgcg agtttctaac attttatgtt ataaaacgta acatgcataa taagataaaa
    14041 aagtggcgaa agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc
    14101 ttttcttttt attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat
    14161 tgagagagat acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca
    14221 gcctgaagcg attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt
    14281 attaggtgcg actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa
    14341 taaaccaaca ctaattattg cccataacaa aacattggct ggtcagctgt acggggaatt
    14401 caaggaattt ttccctaaca atgctgttga atattttgtt tcttattatg attattatca
    14461 gcctgaagct tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga
    14521 tgagatcgac aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat
    14581 cgtcattgca tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca
    14641 agtagtttcg attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt
    14701 cagtatccaa ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg
    14761 tgatgtcgtg gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt
    14821 tggagatgag gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga
    14881 aacggaacac gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga
    14941 gcatgccgta gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga
    15001 aaacaaactt ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat
    15061 gttagaaatg ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa
    15121 agaaggtgaa ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc
    15181 ggacgaatcg cacgtaacca tgcctcaagt cagaggaatg tataatggtg accgagcgag
    15241 aaaacagatg ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact
    15301 tcggttagaa gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg
    15361 accatatgaa catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct
    15421 gcttgaccca gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga
    15481 aataaatgaa cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat
    15541 ggcagaagac ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag
    15601 tgatatcaaa acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga
    15661 tgtgttagtt ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt
    15721 tgctatcttg gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac
    15781 gattggacga gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac
    15841 agattctatg cgtctagcga tggatgaaac atcaagacgc cgtacgattc agcagaagta
    15901 caatgaagaa catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc
    15961 tattacgaaa gaatcggaag atgatacaaa agaagcggta caagtctcgt atgaagaaat
    16021 gacgaaagaa gaaaaagata cgttattgat gaaattagaa aaagaaatga aaaatgcggc
    16081 gaaagcattg gattttgaaa cagccgctaa tgtgcgagat atgattttag aattaaaagc
    16141 ttcaaagtag gaggacaaca tggctaacga taaaattacg attcacggtg cacgtgccca
    16201 taacttgaaa aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt
    16261 gtccggttct ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag
    16321 atacgtagag agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga
    16381 cgtagatagc atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa
    16441 aaatcctcgg tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt
    16501 tgcacgtgta ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt
    16561 agatcaaatg gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc
    16621 accgatcgtg gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga
    16681 gggatacgtc cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct
    16741 ggagaaaaac aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg
    16801 gattcgttct cgtctatttg attctttaga ggcggctctg cgattggcag acggctatgc
    16861 tctggttgac gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta
    16921 ttgcggattt actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg
    16981 agcttgtcca gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat
    17041 tccagataga acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc
    17101 tcagtattat cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac
    17161 accattcgaa gaattaccgg aagagcatca ggatatcatc ttgaatggga cagatgatct
    17221 tttccatttc cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga
    17281 aggggtattg aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga
    17341 gcaaatgcgt ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa
    17401 tccccaagct ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc
    17461 tattcaaaaa gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat
    17521 tgccaaaccg attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact
    17581 agactatcta actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat
    17641 cagattggct acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc
    17701 atcgatcgga ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg
    17761 tgatcttggc aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga
    17821 ttatctgatt gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg
    17881 cactcctgaa gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa
    17941 aaaatcaatc cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac
    18001 tggtgcttca gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt
    18061 agcagtcaca ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa
    18121 atcgttagcg caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc
    18181 cggctacgaa agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc
    18241 gagaagtaat ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca
    18301 aacgaacgag gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg
    18361 cggtcgttgt gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc
    18421 tgatgtctat gttccttgtg aagtatgtca tggcaaacga tataactctg aaacattaga
    18481 agtgcattac aaaggaaaaa gcattgctga tattttggaa atgacagtag aagatgctgt
    18541 agaattcttc aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg
    18601 cttaggttat gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg
    18661 gatgaaactt gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga
    18721 tgaaccaacg acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag
    18781 attagtagat gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac
    18841 agcagattat atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc
    18901 gactggaaca ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa
    18961 aaaaataatg gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga
    19021 atttttgctt gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac
    19081 actgattggt atagacaaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca
    19141 atcggaggag tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg
    19201 gaaaagaaaa acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg
    19261 aagaatttat ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt
    19321 ttaatgaagc atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc
    19381 gctacagtaa aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtgttta
    19441 tcgagacgat gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt
    19501 tgattgcttg tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag
    19561 aatattttat ctgtccatat attgattatt ctttatttga aagactgatc aataaagtaa
    19621 gcttttatga agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag
    19681 aagaaatgtt agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt
    19741 tgaaacctgc aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt
    19801 ttattttaga gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt
    19861 ataaaagtga aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc
    19921 taaatgcgta tgtagatgaa gatcatcaag ttcggatgat gtgtcttggt catccactgt
    19981 tagaagatcc tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg
    20041 aaaaaatcta tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca
    20101 atttcgatat gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga
    20161 gacaaggtcg tagcagtttc tttgtgacat tgaacggctt gaatctggct cgtttcgtca
    20221 cagaagatcg agtgtttaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag
    20281 ataaagcaag attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa
    20341 atgaagacaa aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtgt
    20401 tttatgaaaa agaccggtca atcaagcgct ggttgttgat gaaatatatg ttccataact
    20461 atattccaag attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt
    20521 tcagtatttt aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc
    20581 accgaacgaa agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa
    20641 cagttcctga tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc
    20701 tgctggatga tattgagaaa caaaatttat tgcagccgaa cttcattgtg ttgacttgta
    20761 acaccgcaca ctactttttt gaagaattac aagcggcaac ggatattcct atccttcata
    20821 tgccaagaga agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc
    20881 taggaacaga aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggat
    20941 tcgaaacagt gattcctgat actgttcttc aagaaaaaat caattatctg atctatcatg
    21001 agatcaagga atcggattat ttgaatcaag agctatatta cgaaatctta gaagaagcgg
    21061 tagagcgcct aaactgtgaa aaagttatct taggttgcac agaattgtca ttgatgcatg
    21121 aatttgctga agataaccat tatccagtga tcgatgccca atctatttta gcagatcgga
    21181 cgattgaacg agcgctagct gaaagaagca aggcattgga tacagcatcg gaaaagtaaa
    21241 aaaattggat ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga
    21301 aaaagaaaac tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga
    21361 ggagttcgta attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc
    21421 agggaaaaca gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat
    21481 gccgcctcga ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa
    21541 aatggcttta ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct
    21601 gatcgaaatc gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga
    21661 cgaagaacta gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg
    21721 tttgatcact gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc
    21781 ttccgtgatc attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga
    21841 gtcattcaaa tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa
    21901 atacgggttg ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca
    21961 ttatatcccg gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat
    22021 gtcctttgat gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt
    22081 gcctggatac attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg
    22141 tcaacaccgt tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa
    22201 agtcaatgtg acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa
    22261 atgaaaacct accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg
    22321 ccagttatcc tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt
    22381 gcagatgatg ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt
    22441 gacttgagaa atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc
    22501 caataccgtt tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc
    22561 attgccgcag tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa
    22621 atgatgcatg tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct
    22681 gtctttaaag acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc
    22741 attgaccgtg tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa
    22801 gtcgtttcag caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc
    22861 agtatccttc caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct
    22921 gaaaccgtct atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat
    22981 gccgatcata tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg
    23041 gtcaatacgg aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta
    23101 gtacaagtcg cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc
    23161 gactttttgg aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa
    23221 ttgttccgtt tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag
    23281 aataggatgt catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga
    23341 gaacatgcga aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg
    23401 aaccagcagt ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat
    23461 tctcttttga aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa
    23521 ttaaaaaaga acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg
    23581 gatcttgata tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga
    23641 aattcgcaaa aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat
    23701 aatccggaaa cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa
    23761 gacatttgcg atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat
    23821 ggctatattt cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt
    23881 gcaaccaact caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg
    23941 gtcaatcgtc tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct
    24001 aaacaaatcg aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa
    24061 aaacttcagg aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta
    24121 ggggaaatga ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa
    24181 ataaatgaat ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat
    24241 acatgaagca ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc
    24301 cgtcaaacta taattccatc tttactggag taacataatt tggaaaggct tctattgttt
    24361 ttaatacaat cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata
    24421 aatttttgct taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg
    24481 cctgattaat taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta
    24541 cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc attggattag
    24601 ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt
    24661 tattcagtga taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta
    24721 tacctgaact tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg
    24781 agggcaaaaa tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt
    24841 tttctagata tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca
    24901 ggtctcataa catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt
    24961 tggagaactc cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt
    25021 gagtatgaat tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat
    25081 ggatttggaa tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc
    25141 ggagaagatt tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca
    25201 ggacctgcta attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt
    25261 gcttctttca ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa
    25321 ttattagaac aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat
    25381 caatataata taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt
    25441 gattagaaaa caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat
    25501 gatttggcct ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag
    25561 atattttgat attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg
    25621 aataatgaga aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt
    25681 gtttcatctg ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag
    25741 acctgcgatt acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag
    25801 gattgtgata gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag
    25861 catggcatgg tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt
    25921 attgttacat gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt
    25981 ttatatgggg aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc
    26041 attcgaaact ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa
    26101 gggttaactc ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac
    26161 aatccaccaa tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca
    26221 gtgaatgttg taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt
    26281 gatcagcaaa aatgtatctg aataaaaaag agagcaacta ttaaatgaga ggagttagga
    26341 gttgttagct ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc
    26401 atcttgcgtt tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac
    26461 gacctagctc aacaaacgac catctaatca actaacagca tacataaaag caactatttt
    26521 tgaagtagaa ttgaaatcgg gataacttaa aaagtgattg ggatactagt gtgaatcacc
    26581 gtacataaat attactcgtt aagggaattt gagaaatttt gtatgaaaat ttaagaatta
    26641 gttagtatgc gcaagtcaaa aatatgaata aattatttgg tggagataat ttgctcagct
    26701 tgtcataaga aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat
    26761 aaggaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
    26821 gccgtttata ggtgtttaga gcgtgagaca aaagtgttct ttacttttgt cctacgctcc
    26881 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
    26941 aagatttctt tacccaagat gacagtcgga acaaatcgaa tatgtgcttg ctcagcttcg
    27001 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
    27061 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
    27121 tgtttgatct ctttgatcgc tttttgacca tcgctgattg tgagataccg gtgcatcacg
    27181 tttcctcgct gcaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
    27241 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
    27301 ggacaagata ggttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
    27361 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
    27421 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
    27481 ctaaaaatta gatgaccaat tcaaatagac atatgtgaaa tatttttata aataaaagaa
    27541 aaaatggaat gttttacgtt ataatggaac agataataag agagaatatg aacaaggatg
    27601 atgaatcatg cgaaaaatga aaacaatgga tggcaatacg gcagccgcat atatttctta
    27661 tgcatttacc gagattgctg caatatatcc aataacaccc agttcgacca tggctgaatt
    27721 agtagatcaa tggtcagcag caggaaaaga aaatgtattc ggtcaaccag taaaagtagt
    27781 agaaatgcaa tccgaagcag gagcagctgg tgtggtccat ggatcattga aaactggtgc
    27841 actgacaact acatataccg cttctcaagg gctgcttttg atgataccga atatgtacaa
    27901 gattgctggg gaactactgc caagtgtttt ccatgttgct agtcgggcag tgacgacaaa
    27961 tgcgttgaat attttcggag atcacggaga cgtgatggct gcaagacaaa caggatttgc
    28021 tatgttagct gaaagcagtg tacaagaagt gatggattta gcacctgttg cccatcttgc
    28081 ttctattgaa ggagaagtac cttttttgaa tttctttgat ggattccgta cgagtcacga
    28141 gattcaaaaa atcgaagttt tggaatatga agaattagct cctctgatca atcaggagaa
    28201 attagcttca tttcgtaaac gagggatgaa tcccaatcat ccgactgtta gtggtacgaa
    28261 ccagaatcca gacatccatt ttcaacaacg ggaaacgatc aacccctatt atgataaatt
    28321 acctgaaatc gttcaaaaat atatgaaaga gatcaaccga ttacgaggaa cttcttatga
    28381 cttggtgact tattacggag cagacgatgc ggaagaagtc attgtttcta tgggatcagt
    28441 tgcacagacg attgaacaaa cgattgatta cttgaatgca aacggacgga aaacaggttt
    28501 tttgaatatc catctttatc gtccttttcc agtggaaaca ttcgtgcaaa agttgcctca
    28561 tacagtaaaa acaattgctg ttcttgatcg aagtaaagaa ccaggggcag gtggagaacc
    28621 tctcttatta gacgttcaaa gtgcgttata tgattctgaa cttcgcccgg ctgttattgg
    28681 tggtcgctac ggattggggt ctaaagatgt gactcccgac caaatctcag cagtttttga
    28741 tgaattgaaa aaagacccaa gcatacgcaa aaaacggttt accataggga tcgtggatga
    28801 tgtgacttat caatctttgg aaccaaagga aagtctggat ttgacagaac cgcaaacatt
    28861 ccaggccaag ttttggggat ttggatctga cggaacagtt ggagcaaata aatcagctat
    28921 caagattatt ggagatcaca ccgataaata tgcgcaaggc tatttttatt atgattcgaa
    28981 aaaatctggc ggtctgactg tctctcatct tcgttttgga gatacgccga tacgttcagc
    29041 gtatcttgtc gagcatgcgg accttgttgc ctgtcacaca ccagcttacc tgcatagcta
    29101 tgacttggtc aaaggattga agccaggtgg cattttttta ttgaatactc tttggagcga
    29161 cgaacaatta gaaacgcatc tgcccttaaa attaaaacgt tacttggctg aaaataacat
    29221 tagattctat acgatcaatg ccatgagatt ggcccaagaa gtcggtttgg gacgccggat
    29281 caatacggca atggaaacgg ctttttttaa attagctgac atcattcctt ttgatgaggt
    29341 gcttccgctg ctaaaagaag aagccctgaa aagctatgga cataaatcaa tggctatcgt
    29401 agaaaaaaac atacaagcaa tcgatcggac gaccgaactg cttcatcaag tcgcgatccc
    29461 agaccattgg agaacgttgg agatgcctga aaaaatcaat aaaaggaaca ctggttatat
    29521 acaagaaata cttgaaccag tgaatgcaca ggaaggaaat caactctctg ttggtacact
    29581 tgtagcaaat ggaatgacaa ctggcgaaat gcctttagga acggcttcta tggaaaagcg
    29641 gggcgtcgct ttagaagtac ctgagtggat tagtgatcgc tgtacgatgt gtaatgaatg
    29701 tgcctttgtt tgtcctcatg ctgccatccg tccattttta gcagatgaag aggaaatgga
    29761 agaagcgcca gaaggattta tcgtgagaga aatgcgaggc gcagacggcg taaaataccg
    29821 tatccaagtt tcagtggaag actgtacagg ttgtggtctg tgtgtagagg cttgtcccgc
    29881 aaaagaaaaa gctttagtga tgaagcctta tgaagaacag aaagaacaag ctgtgaattg
    29941 ggcttttgca atgacattaa gacaaaaagc aaatccagtc aaaggaaaga atactgtctt
    30001 gtctacacaa ttcaatcaac cgcttcttga attctcgggc gcttgctcag gctgtggcga
    30061 aacaccttat gtaaaattat tgacacaaat gttcggtgat cgaatgatga tcgcgaatgc
    30121 gacaggctgt tcttctattt ggggcggtgc ttctcctgct tctccttata cgaccaatga
    30181 atgtggtcaa gggcctgcat ggtcaaattc tttattggaa gataatgctg aatttggtta
    30241 tggtatgctt ctagcggcac agactcgtcg cgaggctttg gcaagttcaa tcaaagaagt
    30301 attgccaaaa gtatcagaag aactacgaga attgatggaa gactggttgg tacacatgtt
    30361 tgagagcgaa ggaacgcagc aaagagcagc aaaactgcaa gctgcgatcg aaagcgaacg
    30421 atcaattgtc cctgaactgg aggatatata caagagaaag gatctattcg taaaaaacag
    30481 ccaatggatg attggtggag acggatgggc ttacgacatt ggttttggcg ggatcgatca
    30541 tgtacttgcc agcggagcag acgtgaatat tcttgttttg gataacgaag tctattccaa
    30601 tactggcggt caaacctcaa aagcaacacc agcttcggca atcgctaaat tttcggctag
    30661 tggaaagtat gcttcaaaga aagatctagg aatgatggcg atgacatatg gaaatgttta
    30721 tgtggcgcaa attgcttcag gtgccaatca aatgcagacg ataaaggctt ttgaagaagc
    30781 agaaaagtat cctggacctt ctttaatcat tgcctatacg ccttgtatca cacatggttt
    30841 gcttggtgga atgaaagaat cgcttaaaga agcaaaagaa gctgtttctt ctggctattg
    30901 gtcattatat cgatataacc ctgcaatggt cgaaaaaaac aagaacccaa tgactttgga
    30961 ctacaaaaaa cctgatttct ctgctatgcc tgatttcatg aggaaacaag ttcgcttttc
    31021 ctcgctagag aacgcaaatc caaatcttgc aaaagcttta tttgaaaaga ctatccaaga
    31081 cgctaaaaca cgtttttaca actatgctgc actgacggga gaattagaaa aaatcaagaa
    31141 acgacttgaa ccagaggaat ctatcgatgt acccgttaag actagaaaag aaaaaacgac
    31201 ggaccttgaa gcagaagaaa gaagagctgc cagagcagaa cgacgtaaaa aaatggaaca
    31261 agctacaaaa gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat tctctgaagt
    31321 ttaaaaatat ttattgcaga gaaaaataaa acgcatactt gcaattttcc tgaaaattat
    31381 gcgtttttct caagaaaaat acgagcagat cagcctctaa aattaaaaca tatgatatac
    31441 tagagaggaa atctcaaaag agaagagagg ctttttatgt catttcaaat cggggaactt
    31501 tttaatttgg actattggca gcaaatgttt tccggtgact tgttctctct gaattttttg
    31561 gtgaacatac ttgatattct ggttgtctgg tatcttgtat acaaactgat ccaactgctg
    31621 cgaggcacaa aagcgattca gcttctgaaa ggtgtagcta tttttattgt tatccgaatc
    31681 gtagcggaaa tcattggtct gcacaccttg tcttggctga tgaaccaagt cattacgtac
    31741 ggggtgatag ctgctatcgt gatttttcag ccggaaatcc ggagaggact ggaacatctt
    31801 ggacgcagtt cgcttttcaa acaatcaaaa agcgaacagc aggaagatga gaagatgatc
    31861 ttgtcttttg acaaagccat ccaatatatg tccaagcgaa aaatcggtgc gctaattact
    31921 atcgaacggc atactggatt agatgagtat attgagacag gaattgcatt agatgcagat
    31981 atcactggag agctgctgat caatatcttt attcctaata caccgctgca tgacggtgca
    32041 gttattgtaa aagaaggaaa gatcgcagta gcaagcgcct atcttcctct atcagaaagt
    32101 atgttgattc caaaagaatt cgggactcga caccgggcag ctgtcggtat aagcgaagtc
    32161 agcgacgcga ttaccatcgt tgtctctgaa gaaactgggg atgtcagcat tacattagat
    32221 aatgaactga tggctggctt atcccagcaa gaatatttgg caattttgcg tagagaactg
    32281 attgctgaag agaaaacaaa aaacaagaaa aacgtgattc aatctttcct agacggtatg
    32341 actaagggag gggatcgcaa atgatctcaa aagagaaacg gacaagcatt ctttacagtt
    32401 ttattgcatt gctgttcagt attgcgctgt acttcaatgc aaatggccag tccgttccaa
    32461 atacgctttc tggaaatgaa gcgtataatc aaacagttac tggggtgcca atcaagattt
    32521 cttatgattc aaagcgctac tatatccatg gatatgaaaa tgcagtgact gtcaaacttt
    32581 ccagtgcaaa ccgagttcag ttgaatgcag aggcaaacga agatacacgg atgttccgcg
    32641 tcacagcaga tatcactaag ctgggcgaag gaacacatga ggttccgcta aaaattcaaa
    32701 atttgagcag tgctgtcaca gcaaagatcg aaccatcgac tattacagta acggttgaga
    32761 aaaaggtaat aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc acaccatctg
    32821 gatatgaact tgatgatact tccgttagtc ctaaaaaagt ctctatcact acaggtgaca
    32881 aaacattggc agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg
    32941 atgggattaa tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa gtattaagta
    33001 tcgtctctga tccagtgcaa gtaacagtga gagctgattc gatcaagcca agcaagtcgg
    33061 tacgacttta tgggatacag caaggaacac cagctgccgg ggtagaaagc tatgatttca
    33121 gtttttcaaa actggaagca gaagtaagcg gctctagcga tttgcttgca tcgatcggtg
    33181 acagtattgc tgtaccgatc aatgtctcag gtatcaatca tcgaacaact cgtaaaattg
    33241 aaataccagt agaagagggg ttaagtatca agcccgaatc agttagtgtg gagattacac
    33301 ccgtgttgca aacgtcaagc agtagctctg cttcgacgag cagtcggaca caagaaaata
    33361 caagtacgag tagaagtatt tcatctgctg attcaggaac ggaaccatcg acaacaacca
    33421 tttcttccag ttcggaatct gggacaagca gctcgaccac aagtacagaa gaaacatcag
    33481 aaacaagcga atcaacacaa atgagcagaa caaattcctc agaaaattaa gttaaaggag
    33541 taattttaca actatgggta aatattttgg aacagacggc gtacgcggtg aagccaacaa
    33601 ggaacttacg ccagaacttg catttaagct gggacggtgc ggaggttatg tcctaagcca
    33661 gcatgaagaa gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa gaatctcagg
    33721 tcaaatgtta gaagaagcgc tgatttcagg acttttatcg gtgggtatcg aagtgttcca
    33781 gctaggtgtg atctctacac caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc
    33841 aggggtcatg atttctgctt ctcataatcc agcacaagat aatggaatca aatttttcgg
    33901 tgcagatggt tttaaactag tagatgacca agagctagaa attgaagcat tattagatgc
    33961 tgaagtggat gaattgcctc gtccatcagc tgaaggattg ggaactgtag aagaatttcc
    34021 agaaggctta ttgaaatatt ctcaattcct tgtacaaacg atcaacggtg acctttccgg
    34081 tttgaccatt tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta atcgtttgtt
    34141 tgccgatctt gaaacagatt tttataccat gggcacaaat ccagacgggt taaatatcaa
    34201 tgatggcgta ggctctaccc atccagaacg tttagctgag atggtcgttg aaaaaggagc
    34261 agatgctgga ctagctttcg atggcgatgg cgatcgtatt attgcggtgg atgaacttgg
    34321 aaacattgtc gacggtgata aaatcatgtt tatctgtgct agatacttgg cagaaaaaaa
    34381 tcgattgaaa aaagatacga tcgttacgac tgtcatgagt aacttaggat tccacaaagc
    34441 ggtggaagga atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga
    34501 agaaatgcgc aaaaacgatt ataactttgg cggcgagcag tctgggcaca tgattttctt
    34561 agattttaac acaactggag atgggatgct ttcaggaatt cagttactga atatcatgaa
    34621 acagacaggt aagaaattat cagagcttgc agcagaggta acaatctatc cgcaaaaatt
    34681 agtgaatatc cgagtaagtg ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt
    34741 gattgaagag gcagaagctg aaatgggatc agaaggaaga atcctagttc gtccttccgg
    34801 tacagaacca ttacttcgag tgatggcaga agcgcctact gatgagaaag ttaattacta
    34861 tgtcgataaa atcgcggcag tagtcaaaga agaaatcgga ctggattaag agatattttc
    34921 ataaaaaata aaagaacaag gaccagtgtc agatgtctga cactggtcct tgttctttta
    34981 ttattctact gtcactgatt tcgctaaatt acgtggtttg tcaacgtcga atccccgtaa
    35041 tagcgttgcg taatatgcaa gtaattgagt cggtacgact gtggttagag tagttagata
    35101 agggtggaca gttggtagca ccagctggtc actttccttt tctaatccct ctgagacaat
    35161 aactagtgtt ttagcgccac ggctttcgac ttctttcagg tttcctcgag tatgcgcacc
    35221 agtcacttct tcactgatga ttccaatcac tggtgttcct tcttcgatca aagcaatcgt
    35281 tccatgtttc aattcgccag cggcaaaacc ttctgcctgg atataactga tttctttcaa
    35341 tttcaaagca gcttcaagag aaacgttgta gtcagcgcca cgtccaatgt aaaaggcatt
    35401 tcttgtatcg ctgagatata cttctgccaa ctcttctaaa taatcttttt gggcgatgac
    35461 cgattccatc gcattagcaa ctaaactcaa ttcatgagtg atatcgaata caagagaagt
    35521 ttcaaattct tttttatcac cgatagcttt ggctaataaa gttagtacag ctatttgtgc
    35581 agtataagct ttcgttgatg ctactgcgat ttcgggtcca gcatgcaata gcagtgtaaa
    35641 ggatgcttcc cgcgaaagag tagaaccagc aacatttgta atcgtaagtg aagggtagcc
    35701 gagacggttg atttttacca gaacttggcg gctatctgct gtttcgccac tttgactcaa
    35761 gaaaataaag aatggttttt ttgaaagaag aggtgtgtta tagccaaatt cactggctaa
    35821 atgaacctca acagggatct gtgtcaattt ttccagagtc tgtttacctg ctaaccccgc
    35881 atggtaacta gttccgcaag caacgatata gattcgatcg ctatcagaca atgtatcgac
    35941 cagcttttga tcaagagaga cacgattgtc ttctgttacg tatttttgag cgatacgacg
    36001 catgataata ggctgttcgt cgatttcttt cagcatataa aaaggatagg tacctttctc
    36061 gatatctgaa gaatctactt gtgcttcgaa tgaagaacgt tctacgatgt cacctgcaaa
    36121 attttgaata ataacttttt ctgatgtgac ggtaaccatc tcttcatctt caatctcaac
    36181 gaattccttc gtatgagcaa gcatagccat tgcatcgctg gcaatcacat tgaatccatc
    36241 gccgagtccg attaatagtg gacttttatt tttagcgacg taaataacat caggtgctgt
    36301 ccgatcaatc aaagcgaaag cgtaagaacc tttgatgacc cgcaaagctt ttaaaaacgc
    36361 ctcttgcgtt gtagtatcct tcgcgaaagt ttcaattaga tgagcgataa tctcagtatc
    36421 ggtctcacca ataaagtggt catttgctaa ataagcaatt ttaagttctt caaagttttc
    36481 gattacgcca ttgtgaacga gcacgaagcg tccactttgg gaagtatgtg gatgggcgtt
    36541 ttcttcagaa ggttgcccgt gagttgccca gcgtgtatga ccaataccta tatttcctgt
    36601 agtatcaata tttattttgt ttttcaaatt ttgaattctg ccttgtgctt tgaccagata
    36661 atcagttgtg ccatctgaga caaagatacc agctgaatca taaccacggt attctaattt
    36721 ttctagaccg tcaatcaatc ctggtgttac attttttaat ccaatcattc caacaattcc
    36781 gcacataaaa aacatcctac tttctataat gaattcgtat gaatgcttcc agctgcagga
    36841 aaatcctttt gttttatctt tgcagatatt ttttgtggat tttctgtaga gtgcagtccc
    36901 tagggccatc cgccgaatct ttcgataaac cctctcctcg tcatctgttt acacagacct
    36961 ggcgctatcc taaaaggtat aactatcctc ctcttttgtt ttttatctgg aaacatttaa
    37021 aatcataacc ttttgcgaat gttcttgtca acaaaaaaag aataaaaaat aaattggtat
    37081 atatcaaatg ctttgtcaaa tgctcttttt atgtcaaaaa ataatgggtt tttatctatc
    37141 atctttttca ggagttcggt acaatagaaa gtaatcggat agaggaggag taaaaatgga
    37201 ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa caattgtcca aattcgaagc
    37261 atcagctggt gtatcagatg atacgaaaat atttttagat actggctggg atagcatcca
    37321 agaaatcgca cctgatgcct tggaagttgc tgaagctcgt gagtttacgg tagaagatga
    37381 attgacaaaa gagtcgtttt ccgggtatgc gcgggaagaa aaagcagagc ggttcgacac
    37441 ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg tattagaaag gagaaagaat
    37501 gaataagaga cgttgggcag cagttctgat tgctgcagga ttatttgtgg tttcgttagt
    37561 ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt gagggattga acagttggtt
    37621 atatggggat gaagaactag cttcaaatgt gttggaagag ggagacagta cgcaaaaaat
    37681 cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg tcttcaggtt tatttgcttc
    37741 tgaaggttac aatcataaaa attttatgga acaactgaag gcgattgaag aagatccaac
    37801 agtcaaaggt atctttttag aagtaaattc tcctggtggc ggtgtctacg aaagtgcgga
    37861 aatcgctaag aaattggata cgattcgtaa agaacatgat atcccaatgt atgtcagcat
    37921 gaaaaacatg gcagctagcg gtggctacta tatttctgct caagcagata aaatctttgc
    37981 aacagaagaa acagtgaccg gctcaattgg tgtgatcatg tcaggattga attattcagg
    38041 acttctagaa aaactgggtg tggaagatac aacagttaaa agcggagcgt tgaaagatat
    38101 gggttcagga acgcgaccag agacaaaaga agaagaagcg gtgcttcaag catatatcga
    38161 cagcgcatat agtcgttttg tagcaatcgt cagcgaggga cggaataaat ctgaagaaga
    38221 agtcaaaaaa attgctgatg gaaggattta tgatggtgtc caagcaaaag aagtaggttt
    38281 agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct atgcgaaagg aacaacagct
    38341 tgaagatgcg gaactagtag aatattcttc cacgccatct gggtttgcta gtacatggtt
    38401 aggaagtaaa ctagcagaat tccagggatt gaagccttct gagactagtc agatcttatc
    38461 cgttcttgag aatctaggga cagcggaatc accaaaagca atgtattatt acggaggtga
    38521 gtaagcatgt cagagctttc aaatgaaaat aaggaacaag aaaaagataa gcaacagcag
    38581 cctgctgtag gacctgactc caacttttcc caaaaggtac tgagctcttt tgaaagagag
    38641 cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat ggaaaaagta tacggacaca
    38701 agaaatgaac cgccaaaagc aaatgatttt cctaagtatt tctatgcagg tttttttatc
    38761 cgaatgtttg ctttcttggt cgatcttttg tgtatcggag ctattacgcg tttaacactt
    38821 ggtattgcca aaaatctagg ttggcttaat ttttcagatt cttatctgag tatctacgga
    38881 ttcacagcct tacttattta tctagcgtat tttattttat tgacgaaatt caatcatggt
    38941 caaacgattg ggaaaatgat ctttggtatt cgagtcatca gctttgatga agaagaactt
    39001 agctggtcaa ccgttttagt tagagaaggt gcttgccggt tcattctaaa atttcctttg
    39061 ctgatgttag ggtatcttcc agcaatcttt aatcagaaaa agcaacatct aggcgatttt
    39121 ttcactaata caagtgttgt tacgatcaat ctgatcaaag cattcaacca ggaagtcaac
    39181 agctgatgag caataaaaag cagtaacagt tgaaagcacc ttctaaaagt cagatctaaa
    39241 atctagcttt tagagggtgc ttcagttatt tttaattgtc atgctttttt tgttttgcta
    39301 ctattgaaca tgagctgaac atgagcaaga ggaggacact gcgtgaagcc gattgatttt
    39361 ccagaaaaat atgagaattt aatgcgagta gcacaacaag cactagcaaa ccagcaatat
    39421 cagcaggcaa aagaattatt tcaacgagct tacgagttga aagaaagctt tgaggctaat
    39481 agcctgcttg ttttttgtct ttacgagttg gacgagaaaa aagaagcatt gaagcaagca
    39541 ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg ctgaatttta ttttgacttg
    39601 ttaatcagta cacaagattt tttatatgct agaaaattga tagcaagtac agatttttat
    39661 gaagctttcg agcagcgggt cattgaaaaa atccaatttg cagaagaatt atccggacaa
    39721 atggagaggc agaagatcaa ggcactccac aataaatcgg aggaacttcc ttcgttggaa
    39781 cctgcacgtc agcttgcgtc tattgatgag atcgaacagc ttccttatca tgagtttatc
    39841 caaacagcga gtaaacttat tgtatcgcca gaggtccata ttttagcaag ggcaaaatta
    39901 ctggagacac ttaggcaatt gaacgaatgc catcctgttt tctatttgac gattgaagaa
    39961 aagttagtca aagtcatacc aaaagatctg ccaaagccgc agcagcaggc aagttaccag
    40021 caattatgtg tattttcaga tcgttacggt aatgaagatg cgctgcttag ttccgtgtta
    40081 aatgaagaat tcacgttgca atctgcaatt gtttatcctg tctatgatac atacatcgaa
    40141 gacccgaagc gctggtttca actgactgta gaggcgtgta caggaaaaac gatgtatgaa
    40201 ggtacagaag atgaaaaaca agatttttta aaaaaaagag aaaaaatttt gcagcaaatg
    40261 atcttcttcc attagatttt atcagtgaaa aagataaaaa tgttatgaaa aacaaggatt
    40321 ctatccattc aatatgttta caatttgaaa gggttagtgt actatttaaa ggtatgcatg
    40381 aaacgttttt gatgtataaa atgaatttct aaactcggag ggaattaaat gactgcaaca
    40441 tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta cgattgcgca agacgaaatc
    40501 caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa gcttgaacgt acctggattc
    40561 cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt acggtgaaga agctttatat
    40621 gaagatgcat tgaatgctgt attaccagaa gcttatgaag ctgctgtcaa agaagctggc
    40681 atcgatcctg tttctcaacc aaaaatcgat gttgaaagca tggaaaaagg tcaagactgg
    40741 gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat taggacaata taaagatcta
    40801 gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg tggatgcacg tatcaaacgt
    40861 gaacaagaag cacaagctga attagttatc aaagaagatg ctgcagctga aaatggcgat
    40921 acagtagtaa tcgacttcga aggctttgta gatggcgaag cgtttgatgg cggaaaaggc
    40981 gaaaattact cacttgaatt aggttcaggc tcattcattc caggttttga agatcaatta
    41041 gttggtcata aatcaggtga aagtctagat gtaaatgtaa ctttcccaga agattatcaa
    41101 gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga caatccatga agtaaaagca
    41161 aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg ttgatgacga agtatcaaca
    41221 ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag aaacaaaaga aaaagcagcg
    41281 gacgatgcaa aagatgaagc agcgatccgt atggctgttg aaaacgcaga aatcgttgaa
    41341 ttgccacacg tgatggttca tgatgaagta catcgttcaa tggacgaatt cttgaacaac
    41401 atgcaacgtc aaggtatctc tcctgaaatg tactatcaat taactggttc tacagaagaa
    41461 gacttgcata aacaattcga aggtgaagca gaaatgcgca caagaacaaa tcttgtgatc
    41521 gaagcaattg cagctgctga aaatcttcaa gcaacagaag aagaaatcaa tgcagaaatc
    41581 caagaattat cagaaacata caacatgcca atcgaacaaa tcaaacgcgt gttgacagaa
    41641 gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg aattgatcac aggtacagcg
    41701 acagaagcga aagaatagaa atcaagtaat aaatcatttc tgaagaagct acttctagaa
    41761 aattgtttat tcgaaataag ggactgtgac acgattttgt cacagttcct tattttttgt
    41821 taagagtaaa aaatgcaaag gtataggtca gttgatcctt tgttaaacac tttttctttt
    41881 tactaaacta tgttatgata gtaaaagact gtaaagtctt tgatagtttc ttgcatactt
    41941 tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa gtagcaatga gaccgtacgc
    42001 tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga tcgtagctgg tccaggggtc
    42061 tacatctgta atgaatgtat cgacctatgt aaagaaatca ttgatgaaga attttatgaa
    42121 gaagctgtac gcgaattcac tgaagtccct aaaccactag aaattcttga agtattgaat
    42181 aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag ctgttgccgt atacaatcac
    42241 tataaacgtg tcaatcagca agcacaagaa aacacagatg atgttgaact gcaaaaaagc
    42301 aatatctgct tgatcggacc tacagggtct ggtaaaacat ttttagcaca aacattagca
    42361 aaaacattga atgtaccttt tgcaatcgca gatgcgacaa gtttgacaga agctggttat
    42421 gtgggggaag atgtagaaaa cattttgctt aaattgcttc aatctgctga ttacaatgtg
    42481 gaacgtgcag aaaaagggat catctatatt gatgagatcg ataaaattgc gcgtaaaagc
    42541 gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg tccaacaagc cttgctgaaa
    42601 attctagaag gaactgttgc aagcgtgcca cctcagggag gacgtaaaca tccacatcaa
    42661 gaatttattc agattgatac gacgaacata ttgttcatcg taggtggtgc atttgatggc
    42721 atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga ttggatttgg tacacaaaac
    42781 caaaaacttt ctgaagacga aagtgtcatg cagcatatca ttcctgaaga tttactaaaa
    42841 ttcggattga tccctgagtt tatcggacga ttacctgtta tggcagcttt agagaaattg
    42901 acaacagatg atctggtacg gatcttgact gagccgaaaa atgcgctagt caaacaatat
    42961 caaaaacttc tttctctaga taataccgaa cttgaattcg aacctgaagc gttgagagcc
    43021 attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag gacttcgttc catcattgaa
    43081 gaaatcatga tggatgtgat gtttgatatc ccttcaaatg aaaacatcga aaaagtgatt
    43141 atcacaaaag aagcagctga attgtctggt gagccaacag taatctataa agattcagag
    43201 aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca ttttggcttt tgcgacagcc
    43261 gcttgcttta ttcatcacct tgttaaggag agagaccatg aaagtccatc aagctgaaat
    43321 tatcataagt gcagtggcac caaaacaata tccagataca caattgccag aaatagctct
    43381 ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac acattgattg atcgaaagaa
    43441 tttagcccgt acttccggta agccaggaaa gacccagaca ttgaattttt atttgatcga
    43501 gaacgcatta cattttgtcg atgttccggg atatgggtat gcaaaagtat ccaaaactga
    43561 gcgagcaaaa tggggaaaga tgatcgagac atatttaacg actagagacc agctacgtgc
    43621 ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa gaagatatcc agatgtatca
    43681 atttttgaag tattatgata tccctgtaat tgttgttgcc actaaggcag ataagatccc
    43741 tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa gcgttggatt ttgatcgcaa
    43801 tgacacgttt attctctttt cttccgttac aaaacaggga aaagaagaag cgtgggcagc
    43861 aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga cttaccagtc aaactctttc
    43921 ataaaa
//
LOCUS       DAPBCS010000022        41596 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPBCS010000022 DAPBCS010000000
VERSION     DAPBCS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.022"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..196)
                     /locus_tag="RD521_001368"
     rRNA            complement(<1..196)
                     /locus_tag="RD521_001368"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(877..2373)
                     /gene="lysS"
                     /locus_tag="RD521_001369"
     CDS             complement(877..2373)
                     /gene="lysS"
                     /locus_tag="RD521_001369"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDT7771113.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2484..3488)
                     /gene="dusB"
                     /locus_tag="RD521_001370"
     CDS             complement(2484..3488)
                     /gene="dusB"
                     /locus_tag="RD521_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDT7771114.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3489..4376)
                     /gene="hslO"
                     /locus_tag="RD521_001371"
     CDS             complement(3489..4376)
                     /gene="hslO"
                     /locus_tag="RD521_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDT7771115.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4593..6704)
                     /gene="ftsH"
                     /locus_tag="RD521_001372"
     CDS             complement(4593..6704)
                     /gene="ftsH"
                     /locus_tag="RD521_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDT7771116.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6794..7339)
                     /gene="hpt"
                     /locus_tag="RD521_001373"
     CDS             complement(6794..7339)
                     /gene="hpt"
                     /locus_tag="RD521_001373"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDT7771117.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7339..8784)
                     /gene="tilS"
                     /locus_tag="RD521_001374"
     CDS             complement(7339..8784)
                     /gene="tilS"
                     /locus_tag="RD521_001374"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDT7771118.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8904..9374)
                     /locus_tag="RD521_001375"
     CDS             complement(8904..9374)
                     /locus_tag="RD521_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDT7771119.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9420..9845)
                     /locus_tag="RD521_001376"
     CDS             complement(9420..9845)
                     /locus_tag="RD521_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDT7771120.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9912..10181)
                     /locus_tag="RD521_001377"
     CDS             complement(9912..10181)
                     /locus_tag="RD521_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDT7771121.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(10192..11787)
                     /locus_tag="RD521_001378"
     CDS             complement(10192..11787)
                     /locus_tag="RD521_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7771122.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11802..15323)
                     /gene="mfd"
                     /locus_tag="RD521_001379"
     CDS             complement(11802..15323)
                     /gene="mfd"
                     /locus_tag="RD521_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDT7771123.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15340..15900)
                     /locus_tag="RD521_001380"
     CDS             complement(15340..15900)
                     /locus_tag="RD521_001380"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDT7771124.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            16253..17227
                     /locus_tag="RD521_001381"
     CDS             16253..17227
                     /locus_tag="RD521_001381"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDT7771125.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17397..18797
                     /locus_tag="RD521_001382"
     CDS             17397..18797
                     /locus_tag="RD521_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDT7771126.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18875..19597)
                     /locus_tag="RD521_001383"
     CDS             complement(18875..19597)
                     /locus_tag="RD521_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771127.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19676..20182)
                     /locus_tag="RD521_001384"
     CDS             complement(19676..20182)
                     /locus_tag="RD521_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7771128.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20305..22104
                     /locus_tag="RD521_001385"
     CDS             20305..22104
                     /locus_tag="RD521_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDT7771129.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(22172..23746)
                     /locus_tag="RD521_001386"
     CDS             complement(22172..23746)
                     /locus_tag="RD521_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7771130.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(24001..24510)
                     /gene="mreD"
                     /locus_tag="RD521_001387"
     CDS             complement(24001..24510)
                     /gene="mreD"
                     /locus_tag="RD521_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDT7771131.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24514..25368)
                     /gene="mreC"
                     /locus_tag="RD521_001388"
     CDS             complement(24514..25368)
                     /gene="mreC"
                     /locus_tag="RD521_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDT7771132.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25523..26161)
                     /locus_tag="RD521_001389"
     CDS             complement(25523..26161)
                     /locus_tag="RD521_001389"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDT7771133.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(26162..26812)
                     /locus_tag="RD521_001390"
     CDS             complement(26162..26812)
                     /locus_tag="RD521_001390"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDT7771134.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26825..27724)
                     /gene="rsgA"
                     /locus_tag="RD521_001391"
     CDS             complement(26825..27724)
                     /gene="rsgA"
                     /locus_tag="RD521_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDT7771135.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27889..29958)
                     /gene="pknB"
                     /locus_tag="RD521_001392"
     CDS             complement(27889..29958)
                     /gene="pknB"
                     /locus_tag="RD521_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDT7771136.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29955..30695)
                     /locus_tag="RD521_001393"
     CDS             complement(29955..30695)
                     /locus_tag="RD521_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDT7771137.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30708..32066)
                     /gene="rsmB"
                     /locus_tag="RD521_001394"
     CDS             complement(30708..32066)
                     /gene="rsmB"
                     /locus_tag="RD521_001394"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDT7771138.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(32066..33004)
                     /locus_tag="RD521_001395"
     CDS             complement(32066..33004)
                     /locus_tag="RD521_001395"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDT7771139.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32997..33488)
                     /gene="def"
                     /locus_tag="RD521_001396"
     CDS             complement(32997..33488)
                     /gene="def"
                     /locus_tag="RD521_001396"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDT7771140.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33503..35911)
                     /gene="priA"
                     /locus_tag="RD521_001397"
     CDS             complement(33503..35911)
                     /gene="priA"
                     /locus_tag="RD521_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDT7771141.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(36164..36469)
                     /locus_tag="RD521_001398"
     CDS             complement(36164..36469)
                     /locus_tag="RD521_001398"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7771142.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36474..37088)
                     /gene="gmk"
                     /locus_tag="RD521_001399"
     CDS             complement(36474..37088)
                     /gene="gmk"
                     /locus_tag="RD521_001399"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDT7771143.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37575..37859)
                     /locus_tag="RD521_001400"
     CDS             complement(37575..37859)
                     /locus_tag="RD521_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771144.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37877..38767)
                     /locus_tag="RD521_001401"
     CDS             complement(37877..38767)
                     /locus_tag="RD521_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDT7771145.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38787..39005)
                     /locus_tag="RD521_001402"
     CDS             complement(38787..39005)
                     /locus_tag="RD521_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDT7771146.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38975..40675)
                     /locus_tag="RD521_001403"
     CDS             complement(38975..40675)
                     /locus_tag="RD521_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDT7771147.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40942..>41596
                     /locus_tag="RD521_001404"
     CDS             40942..>41596
                     /locus_tag="RD521_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771148.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     "
BASE COUNT        11283 a         8975 c         7541 g        13797 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttgtt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaagc gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcggcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttctttttt aacgactcag ttatcttatc atgtcgttag taacatgtca
      601 ataacttttt tgttatgttt ttgaataaat attttcttct gtcttttgga caagtaataa
      661 tataccaact aaatttagct tggtcaacga attttttgta ttttttcaag tttttttcaa
      721 taaacgatta ttcataactt ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta
      781 gaatagtcgg cattagcttt tgaactttcg ttattattta ttcctttcta cacagcaaaa
      841 gctgcctaag gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa
      901 aacatcccga attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc
      961 tagaccacca gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc
     1021 gtgagcttcg tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg
     1081 atcaattgga tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag
     1141 ttcgaatcga tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc
     1201 gactggatgt ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa
     1261 gaattcattg ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc
     1321 ggctaaagct aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc
     1381 tttgattgca tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc
     1441 atcatatgtg atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc
     1501 tgtcagattc attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa
     1561 ttctggatta tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac
     1621 tttttccatt ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata
     1681 taaatcaatg tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc
     1741 ctcattgtgc aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg
     1801 gatttcgctg ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat
     1861 taaatccaag tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc
     1921 tggcagtgga cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc
     1981 gcctgtgtct gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg
     2041 tttgaatact tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg
     2101 gccttcacga tcttgtagat gcgcaaatcc agctttccct tttcctcggc gagtcatcat
     2161 acgaccagct acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc
     2221 aaatagttca tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc
     2281 gattccatct tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa
     2341 atcttctact tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc
     2401 tctttcctat aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg
     2461 aaaattcacg aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt
     2521 tctacaaatt gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct
     2581 actttcactt tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat
     2641 tctctacaag ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct
     2701 gtagcaattt tttcggctgg tgttggttca ggaatcagtt cccctgtttc gagataatgc
     2761 tgtgtgcggt gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct
     2821 gcaccgacat agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc
     2881 ataaatggaa tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct
     2941 tcatacatct gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt
     3001 gctgctaatg cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg
     3061 attgttactg ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca
     3121 ggatctaaaa gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc
     3181 atattgatat ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact
     3241 agactatctt tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg
     3301 tggagcatct ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca
     3361 catacgacta agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt
     3421 ccagccatcg gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg
     3481 tgttccactt aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta
     3541 tacttgtatt tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc
     3601 attgcttgga tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa
     3661 cagtcacatt tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc
     3721 aatcctttca atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt
     3781 gtcgttttta attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt
     3841 tggatcatga atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt
     3901 ccaacagctg aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt
     3961 tctccagaaa caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg
     4021 acagtaaaca ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt
     4081 agaggaagac tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca
     4141 tctactacaa ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct
     4201 cctttgagcg tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag
     4261 gaggtgtgcc aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg
     4321 gcgtatgctc ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt
     4381 tatgtactcc tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt
     4441 ttcaactttg gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca
     4501 ccctatttac aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg
     4561 gagtcatatc agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta
     4621 tcttctgatt gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt
     4681 tttgcttctt caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa
     4741 gcacgttttg cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca
     4801 gtaccttcag gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt
     4861 tcgaattcca aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt
     4921 tctcgagctt tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa
     4981 gcgacttgtt ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg
     5041 tttccttcat attgaacagg acctaaacga tcactcattc catattctgt taccatgctt
     5101 cgtgctagag ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag
     5161 atgatttctt cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct
     5221 ttcgtcatca agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg
     5281 cgagggatga tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc
     5341 gtatgaccag cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct
     5401 tttttagctg gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt
     5461 ttctttttat ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct
     5521 gctccggcaa aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc
     5581 ggtttattcc gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca
     5641 actaaaatct ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa
     5701 cggttggttg cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca
     5761 agcaattggt tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt
     5821 tgacgaccta ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct
     5881 gtttcaaaca aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca
     5941 gaacccgaga tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag
     6001 gttttacctg ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat
     6061 tcaacaaatc ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct
     6121 tcttccgcac ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg
     6181 gcttttgatt taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt
     6241 cccatcatca tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg
     6301 gaaacccata taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt
     6361 gccagttctg ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg
     6421 gtagtagtcg tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc
     6481 tgttctttgt attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct
     6541 ttgatcttgc ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc
     6601 tggccgttat taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca
     6661 taatacaagg catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag
     6721 cgtcaattta atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa
     6781 ttgagataca gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa
     6841 ggttacgata tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt
     6901 tgaagccaac ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg
     6961 taacaatttt gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg
     7021 ttcttccgct gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat
     7081 ctaagtcttt gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa
     7141 catccatgaa atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg
     7201 gtaccgctcc ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta
     7261 actctttgcc tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct
     7321 caatatcctt ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag
     7381 aagtatgtag tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg
     7441 tatcaagcta tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga
     7501 attaggtact ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa
     7561 tttaatgcgg tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca
     7621 aatgtcatga gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg
     7681 tttgaccaga ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa
     7741 caagcgctgg tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa
     7801 atgatactct cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt
     7861 ctgttgccac tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt
     7921 tgagaatgct ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc
     7981 ttcaatcatt tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat
     8041 caactgtccc atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg
     8101 ctggaaatgt ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg
     8161 aagccgattt ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg
     8221 cgattccgct gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag
     8281 tctgcccgta gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc
     8341 acgaacgatt ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa
     8401 cgtgtcatat tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc
     8461 ttctgtttct atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt
     8521 cttctcttca caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg
     8581 gtgattgatg tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa
     8641 atggagcaaa accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg
     8701 ttgccagaat ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt
     8761 tttcccgcct ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc
     8821 gggaaaacgt ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc
     8881 gcactatcat tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct
     8941 tctgtgtttc gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc
     9001 aatgaatcga agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt
     9061 gctggacgat ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct
     9121 tctccagatt cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca
     9181 tcatttactg aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg
     9241 tcttttacat agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct
     9301 agatcaataa acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct
     9361 acctcgattg acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct
     9421 tattttgcag ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt
     9481 gattctaata taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca
     9541 agtttagcta catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg
     9601 tctgcgttga cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc
     9661 atcctttgca tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca
     9721 acaaaaattg cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat
     9781 tcagcatact ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc
     9841 gtcacttgga ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa
     9901 gcttgtctaa tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt
     9961 ttttgtgcat cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt
    10021 ttgttgccaa actggacttt caaaatatcg cctactttga catcactgga agatttcgcc
    10081 aatttaccgt tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt
    10141 ttgatgattc gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt
    10201 aattttttta tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac
    10261 tgtaaacaga cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc
    10321 tatcacgctg acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa
    10381 tacaccataa tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg
    10441 tccaaaatgc cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata
    10501 ccaaagcgtc acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa
    10561 accggtcaga aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa
    10621 agaagggcga aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt
    10681 gaaagcaatg gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa
    10741 cgcataattg atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc
    10801 ggtcgtcaat ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt
    10861 caaatgacga gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc
    10921 taattgtact aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc
    10981 tgacagtccg taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa
    11041 gataagcaat ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa
    11101 gggctttgac ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat
    11161 cttttgttca tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact
    11221 cattgctgcc gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact
    11281 acagacaatc acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc
    11341 catcaagaat cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa
    11401 ggtaaaggaa actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat
    11461 ccagctgcta aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg
    11521 ataaagattt tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt
    11581 tgatatgaac tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata
    11641 aacttgctga taaacataaa aaccttcgtc gccgactaag ttttggaggg gaattcggta
    11701 aagggcactt aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat
    11761 gacgcgccgc atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt
    11821 tgctcacgca acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt
    11881 tggttgggga tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt
    11941 agttttgtcg ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact
    12001 ttacttagag taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga
    12061 tcgccatcca ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct
    12121 ccaaaacggt cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca
    12181 cgaattcgtt tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga
    12241 tacgcatcga caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct
    12301 tgtttacgag aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca
    12361 ataaatccat gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc
    12421 atcgcaatct tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt
    12481 tcactgactt cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg
    12541 ctgcttcttc ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg
    12601 ttctcaacga acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta
    12661 gtgacaagaa catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc
    12721 tctgtcattt gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct
    12781 tctacttttt gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt
    12841 cccatttctc gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt
    12901 tggatcggat aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac
    12961 atcgacatat gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt
    13021 gcacgtaact gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg
    13081 atcagtagac ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca
    13141 acaatatcaa cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt
    13201 ctgaaacgac ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt
    13261 tcatagtgct gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc
    13321 tttatcgctt taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct
    13381 acgagtagtc gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt
    13441 ctcaactggt catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg
    13501 tcatcaggtc caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga
    13561 tcgtcagcga tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt
    13621 ccgcctagct tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga
    13681 ttcagttgag taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag
    13741 tcttggtgaa cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga
    13801 ttggcatgga caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg
    13861 tttgttactg tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct
    13921 gtgatagcta ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact
    13981 aattgtacct gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca
    14041 tctcgaagca tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca
    14101 gtctgttttt gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag
    14161 aactgttgca tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc
    14221 cctttttgga agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt
    14281 atatctgctg aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc
    14341 cattctgccg cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca
    14401 acgactagta aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga
    14461 agatctgagt agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc
    14521 tgaccgaagt aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt
    14581 ttttctagca ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact
    14641 tgttcactcg ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct
    14701 gcttcaaaat accgcataga atctatctct acatcaaata gttctacacg aacaggatag
    14761 gttgtcgtta acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt
    14821 gcgaccatct cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg
    14881 tctatctcat caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc
    14941 agtcgtttgt gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt
    15001 gcgttaagcg ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca
    15061 gaaagcactt catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga
    15121 tcttctgcta actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt
    15181 gttttcttaa acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt
    15241 agttgtctag gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt
    15301 ccgactaatt gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc
    15361 atccacgaac gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct
    15421 ttcttctatc aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt
    15481 ttttttccct tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa
    15541 atgggaaata atacttttga taccgttatg cccgccggca cttccctttt gacggagccg
    15601 gatttttccc actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat
    15661 cccaaaataa gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg
    15721 tttgactaaa aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg
    15781 attttttttg aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat
    15841 attgtgtttt gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat
    15901 ttttgttttc cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc
    15961 cagtccatcg agcttttttc ttttttacac ttgtcaatgc atagtataac acaaaacaag
    16021 gcgtttcggc taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag
    16081 tttaatgaaa taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata
    16141 taatacaacc ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga
    16201 aatgttagca taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc
    16261 agctgcagaa aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc
    16321 tagctatgcc tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat
    16381 caatactgct aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc
    16441 acctaaaaaa atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat
    16501 tacagctggc gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct
    16561 aaaaatcaac cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt
    16621 agttgccgct aacccagtag atattttgac ttactcaact tggaaattct caggtttccc
    16681 taaagaacgc gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact
    16741 tgcggaatta gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg
    16801 agattcagaa ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg
    16861 ggtaaaaaac aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg
    16921 tgacgctgct tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc
    16981 attagcacgt atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt
    17041 attcatgaat ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa
    17101 ccgtcaaggt atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat
    17161 ggctgcttct gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc
    17221 tgaataatct ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta
    17281 tcacccttat ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta
    17341 aattatgcta tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga
    17401 caagatcttc gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc
    17461 tcttagtgct gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc
    17521 cgaatacaga aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa
    17581 aattaaaaga agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc
    17641 aggaagtacc aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga
    17701 tcctaaatga acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac
    17761 aagaaataga tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga
    17821 caactaagct gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa
    17881 aagcaggtga ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg
    17941 ctgcggtcaa acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg
    18001 aattcaaaga aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat
    18061 ccatgaatgc tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga
    18121 tcccatcaag tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata
    18181 cagaacaagt acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg
    18241 acacaaaatt taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt
    18301 ggacgatcca aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag
    18361 attttactcg ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag
    18421 atacgtatat cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag
    18481 tggcccttga aacagatatc gtttccggaa agccaactac acctacacct gctggagtct
    18541 tctatgtttg gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac
    18601 cttacgagag tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg
    18661 attcagactg gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct
    18721 gcatcaacac cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc
    18781 ctgtattggt cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca
    18841 ggtgctttct ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt
    18901 ctgaaacatc atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt
    18961 cctcatttaa atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga
    19021 actctttttc caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga
    19081 atacttcagg agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg
    19141 tctcttcatc aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca
    19201 agtaactgac tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga
    19261 catttgtcgc tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac
    19321 ccggaccata tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag
    19381 cctttttaat cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca
    19441 caacaagccg caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat
    19501 aaatttcacg agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc
    19561 ttccttggat attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt
    19621 gagaatacac ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata
    19681 aacgcttctt gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg
    19741 aatccaacat gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt
    19801 cgtccaaacc tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa
    19861 tttttagctt ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga
    19921 aatgtgcgcc cttccttaaa aagaactcta aaaatttggc gtagcccaga tggttgatac
    19981 tcaccatact tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat
    20041 taggcagcca gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac
    20101 tgtaaaataa gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca
    20161 tttgtttttt tgaagaattc ataagtcacc tctgtatttt atcttagact ttttttgaag
    20221 aaacaacaat cattttcata gagacttctt ccataaaaaa gtgctatact taattttgta
    20281 ttacaatatg aagggagttt tttcatgagt tattcattaa attggaatct agattctatt
    20341 ttttccggag gcagtcattc tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa
    20401 atgaacgagt attatcaccg cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag
    20461 ttaaacgcta ttcttcaatt gcaagaaacg attacgaatg gttttaccca atgcagcagc
    20521 tatattactg cattgctttc tgccaatgtc aatgattcag atgcaaaggt cttatcaggc
    20581 aagctttacg caatgctgcc tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt
    20641 gcagaaatat ctgacaatga ttggaatcag atgctttctc aatcttcgtt tgaaacaatt
    20701 gcttttcgtt taaacgagat tcgtcgtgat ggctcacaat tattatcaga agctgaagaa
    20761 aacattataa atacgctttc tttagatggt ctaaatgctt ggagcagtca ttatgatacg
    20821 attgtagcca gcatttcgat tcccttcgaa caagatggac aagtagtcga attatctgct
    20881 ggacaagcct tcaataagat gatgggcgat ccagatccaa aagtcagaga aacacttttc
    20941 gcagcatggg aagaagcctg gaaagaaaag gcacccctct tcacggatac tttgaatcat
    21001 ttagatggtt ttagactttc tgactatgaa cttcatggtg tcacagattt tctacaaaaa
    21061 ccattagagt acaatcggtt gaagaaagaa acattgtctg tcatgtggga tacaatccag
    21121 aaaaacaaac aaccccttgt taattacttg actagaaaag ctaatctttt cggtaaagaa
    21181 aaaatggaat ggcaagatca agatgcacct attattttag gcgatttgaa agaaaaaacc
    21241 tttagttttg acgaagcagc agcatttatt atcgaaaact ttaacaaatt cagtccaaaa
    21301 atggctaaat ttgcccaatc tgcttttgaa aagagctgga tcgaagcaga agatcgtcct
    21361 gggaaacgtc ctggtggtta ttgtactgag ttaccagaaa caaaagaatc aaggatcttt
    21421 atgacctaca gtaattctgt taatgaggtt gcgacacttg ctcatgaact aggacatgct
    21481 ttccatagca gcacgatgtg ggatttacct tctttaaatc gcgaatatgc catgaatgtc
    21541 gcagaaacag caagtacatt cgcagaactg atcgtagccg atgctacatt aaaagtcgca
    21601 aaaacaaaag aagaaaaaat caatttgcta gatacaaaac tgcaaaatgc tttagctatg
    21661 ttcatgaata tccattcccg attcattttt gaaaatcgat tctatgaagc acggcaagaa
    21721 ggattagtct cagaagataa gatcacagag atgatggttg aggcgcaaaa agaaggctat
    21781 caaggagctt tagctactta ccatccatat ttctgggctg caaaattgca tttctttatt
    21841 gatgacgtac cgttttataa cttcccatac acatttggct acctctttag tttgggcatc
    21901 tatgcttacg ccaaccaaaa gggagcaaat tttgaagaag aatatatcca tttactacgc
    21961 gatactgcct caatgacgac ggaagatctt gcgaagaaac atttgaatgt cgatcttacc
    22021 caaccagatt tttggcaagc aggtatcgac atggtgactg aagatatcca aacgttcatg
    22081 gaattgacag aagaatttat ctgatttcta ttttatcaca ataaaaaaag agttagtcca
    22141 gaatgggcta actctttttg attaatcatt cttacatgct gacagcaaag tcaggggcaa
    22201 accattgaac tgaaccaact tttacgcttt cgcctggttg aggagcatgg atatattgtc
    22261 ctccgcctaa agcaatcgct acgtggtaag ttccgccttg tgacccccag aataacaagt
    22321 ctccagcttt tgcttgagaa actgaaattt ttgttccagc tgattcttga ggaactgtcc
    22381 aaccaccgat gtcacgacca gttacttgca tgtaaacata gcgtgtgaac cctgagcagt
    22441 caaagccact tggatcttta ccgccccaaa catatggagt accaatatat ttgtaagctt
    22501 ctgctacgat tgctgcacca ttcacacttc cagaaggagc tggagcaggt gttacaggtt
    22561 ttggttgttc aggtgttggt gttggcgttg gagccggagt actacttcct gtgccgtttc
    22621 ctgtatcaac actttgatct gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg
    22681 ttgttgaaga ttcaggcact gttgttgatt cttctgttgc tgaagattca ggcactgttg
    22741 ttgattcttc tgttgctgaa gattcaggcg ctgttgttga ttcttctgtt gctgaagatt
    22801 caggcgctgt tgattcttct gttgaagatt caggtgttgt tgattcttct gttgctgaag
    22861 attgtgtagc tgatgatgat tcttcagtca ttgaagattg tgtagctgat gattcttctg
    22921 ttgctgagct ttgtgcagta gatgaagctt gagtagcttg tgcttcagcc tctgcttgtt
    22981 cgcgtgcttc tttttcagct ttttcttgtg cagcttgttg acgagcttgt tctgctagac
    23041 gttgttgttc acgaatacga gcttgctctg cttcagcttc tgctttttga cggttcaagt
    23101 cagctttctt gtcttcagca gttgcttgtt cagcagctaa tgaagttttc agtacgttca
    23161 aatcagcttg tttagataaa agatcgcctt tttgagattc caaagcagct tggttttcag
    23221 ctaattcttt tagtttagca tcgttttcag cttttttgtc ttctacagct tttttatctt
    23281 gtttttgttg ttccatcaag tcgttgtttg cttttaccat cgttgtcatt gcttgaacgc
    23341 gtccaatagc atctgctaat gaatcagcat tcaatacagc atcgatgtag tttgagcttg
    23401 tgttgcttac ttgtgcttcg cgtgcttgtt tttggattgt atcttcacgt ttttcaatac
    23461 gttcttgtaa atctgcaatg tcttttacta attgtgcaga ttcttgacgc aatgtatctt
    23521 gttttgctaa taggtcttgt gcttgtgtgt tgatttcaga aacttggctt tctaaggcat
    23581 cgatttgtga ttgtgcatct gcttgttgat ttttcaagtc tgcaatcttt tgatcttgct
    23641 gttgtatctg agaatcaaaa tcgtccgccg cggcagcgat cggtgatgct acggcagtca
    23701 aggtcattga acataccatt actgctgata ttaaactctt tttcactctt cattcctccg
    23761 actggcttaa tttaataaat atctaaatat atttaagtct ttcttaacga tataaaaatt
    23821 gtaccatagc agtgtgtcaa cgatataata gttatgttac aaaaatgttt caaaacaact
    23881 tttaaaaatg acaaaactgt tcgtttcaga aaatgatcat gaaaaagccg cgttaagtct
    23941 agcatttctt aagataacaa cgattctttt tcgctttcga cctaaaaata aaaaaagaaa
    24001 ttattcatca ctgaataatt tcttaaaggg aaaaatgaat aatacaaata taatcatgtt
    24061 caacagcagt gtaggtccta aaaaccttgt aataaaatac gtgttattta ctactgctaa
    24121 tttaaaaatc aactggacca ccatcgtaaa caattcataa cccgtgacaa agatgatcat
    24181 actgaaaaat tcagtaaaga tgttgacatg gataacgtct ttcattacat acatcaacca
    24241 tacaattaaa gggagagcta ctgcatagat accgataacg ccaatatagt aagcgtcata
    24301 gatagccccg agaacaatcg tggtaatcag taaataacgt ttttcaaacg ccatactgca
    24361 acataataat gccaatatca gaaagtgggc atttgacata taggtgcctt tcgaccactc
    24421 ccctagcatt cttgttaaat gaccatctat caacatcaaa aagaacaaaa cgattggcag
    24481 atagtacttc attgtttctt tcttcaacat ggactaatcc tccgctagcc gttggacaat
    24541 cgtcacaact gacacgtcat acatttctgc ataaggtttc acataaactt cccgatccag
    24601 cccataacta tctggtttcg ttttaataac cgtaccgatc ggcaagttag ctggagaatt
    24661 ccctccaaga ccggatgtct ggacaacatc cccttctttg atatccgtat caccagttaa
    24721 ttgggtcacc actaaggcat ggagcttttc atcatagttt ttaagcaaac caaacgcttc
    24781 gccattagcc gaagataccc gtactggaaa atgattggag ctttcattag aggatgacag
    24841 taattcgatt ttagacgaag ccgtattgac ctcgatcact cggccaatca atcctttttg
    24901 cgacatgact gccatatttg cttcgatacc gtctttagaa cccttgtcta cgatcagcat
    24961 gtcctgccaa ctgtcaggcg aacgagtaat gacatttgcc gttacttttt cgtaactagt
    25021 cagcgtctga ttcaactcaa gttctttttt caacgcttcg atttctctct cgtaattctt
    25081 gttttgctgt gaaacgtcat catagctatc gattttttct tttagccgct cattttcggc
    25141 ataagttgaa aaaagattat ggacagaatc tactccattt cgtacccacc gtactggggc
    25201 agagattgtt ttatcaacca ttgcaacact gtcattaacg atggattgga agaaattggg
    25261 ctttccttca ttcgctcgct gcgcagcggt cacacttagt atcgtcacca cgatgataac
    25321 aataaccaag gtaataataa tatttttatt aggattgaac ttcttcacaa agacacctcg
    25381 aattaattag actcttgtgt tcaattttat cactaaatgc cgtgaaacaa aaggtaagtt
    25441 gctcctctta agcttttttt tagcaaataa tagattctgt catttttagc ctgaaagaca
    25501 aattttttta tttgtttatg gatcaatctt tgctttgaat aactgcaatc ataccatctg
    25561 aaaaagaaaa ttctgcggta tctgaaagaa attcgttact tgcataagat gtcggatatt
    25621 ctacttcttt attttccaat acatacttgc tgccttttaa tgtaagattc gagacgggag
    25681 tcaagcagca ataagctaaa tacttcatat ctggtatctg tcttactata tgctctccag
    25741 gcaaataata agacaaataa ttttgacaat ctctgataat aagctgatgg gcaaagggtt
    25801 tgaagcgagg ttccatacct aaccagagat tggctaaaag atgatccaat cttccgcccg
    25861 tagctccaat caaagtgact tccgcttgcg ggtaacgttc aaatgttttt aagataccta
    25921 actgtgtatc tgtatcgtct ttttctgcag gggaacgggt atactctttg gcttttgcaa
    25981 atacttcctt ctgctcttcg ttcgacaatg aatcaaaatc acccactgcc agatctactg
    26041 ggtagcctgc ttgaagcagg tacagcgcac cacggtctat cccgacatag gcatcgtatt
    26101 tggacagcag agactgcggc cagttttgcg gattacctcc agcagctaat aaaattttca
    26161 tttagtctaa tgcctctttt aatcgttcga tttgtccgac tggattttcg gctccataga
    26221 tatatgatcc tgcaacgaag acatccgccc ctgcatcttt acagattttt gctgtttctg
    26281 gaacgattcc gccatctact tcgattgcat aagtccaatt gttctctcta cgtagatcag
    26341 cgagttccgt aattttttct actgtttctt caataaagct ttgtccgccg aacccaggat
    26401 tgacagtcat gactagtact tgatcagcca aaggcaaaac atgtttgatc atggagactg
    26461 gtgttcctgg attgataact actccagctt taactcctaa gctttttgtc atttgaagtg
    26521 cccgatgaat atgaggagtt gcttcgacgt ggacagtaat gatatctgct ccagcttttg
    26581 caaatgctgg aatgtagttt tctggatcaa cgatcatcag atggacgtct aacggtaatt
    26641 tagtaacagg acggattgcc tgaacaatat ttggccctaa tgtgatattt gggacaaatc
    26701 ggccatccat tacatctacg tggatgtaat ctgcgcctcc tttttccact aactcaatgt
    26761 ctctttgtaa attggcaaaa tctgcactca aaatggatgg tgctatcttc attcttgttt
    26821 cctcctattt tcgtttacct tctttttttg catacattgg ttttcttttt tcgatttctt
    26881 gtaagaattg caaataattg tcgtaacgtg tctgtgcgat accacctgtt tctactctat
    26941 gttttacttc gcagccaggt tctttagcat gcatgcactc tctaaatttg caatggacag
    27001 aagcttctac aaattcagga aattgtctcg gcagttctcc tgcttcaatt gtcaaaaagt
    27061 caatcgagct aaatcctggt gtatctgcta ctaacccgcc atagatagga atcagttcca
    27121 catgacgtgt tgtatgccgt cctcttccaa gtgcatcaga aatctctcct gttgctaaat
    27181 tcagttccgg tgatatttga ttcagtagcg tcgattttcc tgctccagac tgacccataa
    27241 agaccgatag ccgttctggg aagtaacgga tcagttctgt cgtatcaccc gtatgattgg
    27301 gtacaatcac cggatagcct attttagtat aggtttgctt gatctcttcg attttttctg
    27361 ggtcgtctga caagtcggtc tttgtcaaaa aaataatcgg ttcgatcgac tcgtattcca
    27421 aggtaactag aaagcggtca agtagattat aagaaaatgc aggctctacg atacttgtga
    27481 caatgattcc aagatcaaca tttgtgactg gtgggcgaac taattgattt tttctcggca
    27541 agatttccag aagatagccg tctgtttgat tttcactctc aaagatcacc tgatctccga
    27601 caagcggcgt gattttccga ttccggaaat ttcctcttcc tcttgtttga taggtctctc
    27661 cgtttgcgta tacatagtaa aaaccgctga ttgcttttct gatttgtcct ttgataagtg
    27721 ccacctccgt ttttgtttat tttaccatac gaagaggaaa gtaggtgtta ttccatgcaa
    27781 acttctcaaa gtatatggct tgaaaattga tcaaaagaga tagaaacaca aaaaagagaa
    27841 tgcaacaaat caatgtcgca ttcccttctg atatatttta ttcagcactt attttgaact
    27901 ttctgaggtg ctagctgatg aacttgtgga gctggaagat tcttcttttg aagagttgct
    27961 tgtgctactg gagctacttg agctgctaga actgtctgaa gagtcttttt cagaactaga
    28021 agacgctgga tctggtcctt tagagtaaat tacgttcaca accattcctt tggtaattgt
    28081 cgttcctgcc cctggtactg tacgaatcac tttatcttta tccaccgtgt ttgaatattc
    28141 ggaagtctca tgcccgatat attctgcacc gacactagct aaataacttt gtgcttcgct
    28201 ttttgtatag ccggagatat cattcaattt aggctctgtt ccttggctta cgacaaaatt
    28261 gatgtaagtt tcttctgcta caacttcacc cgaggctgga tcctgactga tgatcgaacc
    28321 tgatggtaca gtgtcgctat agtcataagt agcactaata ttattgatat tgaatccttg
    28381 tgtaacaagc caattacgaa cagtttcgta gttttctcct acatgactac tcaaagtcac
    28441 tgtttctggt cctttgctga ctgtgaaagt aatcgtgtct gttttaggat cgacttctgt
    28501 atcttctgat ggagattgcg aaatgatcat tccgctttct acctctgaac tgaattcctc
    28561 ttctttttga atcaagtcag cagaaaaccc taattctttc aaacttgctt cggcatcttc
    28621 ataactttta ccactgtaat catctaattt gatttttttc gtgccgctac taatatacag
    28681 agtgacttcg cgtttttgct tgactgtcgt tcccgctgcc gggtctgtct tcactacatt
    28741 accttcttcg attttgtcat ctgcaatttc ttctgttttt gttgctactt ctaatttcgc
    28801 ttttgcgagt gtttctctag cagaagcttc agaaagatca gaaacatcag gaacaacaac
    28861 ttctcctcgc ccgccagaag caaagaatac agctgttccg attcctaaaa cagcaagaat
    28921 agctaatagg atcagccacc attttttctg tcctttcttt ttaggatcag gcagaacttc
    28981 ttcctcctct ggtaaggttt cgttttctgg cttgtctttt ggcagaggca tagatttgaa
    29041 tgaatcaggc attggtgcat cttccgtaag gggtgtgata acttttgttt cattatccat
    29101 cacatgaggc tgccattttt cttcgtttgc acgagctggt gataatactg tagaaagatc
    29161 atcagacatt tcctcggctg acttataacg gtctgcaggt tctttagccg ttgcccgtaa
    29221 aatcacgttt tccagtgctt gtggcacatt tggatcaagt gcttttatag atggaagatc
    29281 atcttgaaaa tgttttaaag caattgttac agcagattca ccatcaaacg gtacgctacc
    29341 cgtcaacatc tcataaagga tgatccctaa agcataaata tctgactgct tcgttgccat
    29401 acttcctctg gcctgctcag gggacagata atgaacagat cctagcatcg tattcgtctg
    29461 cgtgatcgat gtttcagata aagcgatagc aatcccgaaa tccgtgattt ttacaacacc
    29521 ttcattatcg atcagtacgt tttggggttt caaatctcgg tggatgattt gatggttatg
    29581 agctaaagaa atagcagaaa ggatctgctg cataatattt actgctgttt cataaggaac
    29641 aggataatgt gtttggatat atcgtttcaa gtccattcct ttgacgtatt ccatcaccag
    29701 atactgcatg ttgtcttctt caccaacatc gtaaacacta acaatgtttg gatgaacgag
    29761 ttctgttgct gctaaagctt ctctctgaaa tcgtcgaatc gcagtctggt cattttgaaa
    29821 gtcaaatcgt aagactttaa ctgctacatc acgatccaag attaaatcgt gcgccaaaaa
    29881 tacatttgcc ataccaccgc taccgatatt tccagtgatt tgataacggc catttaattt
    29941 tttgcccaac tcgatcatac actgtcctcc ttatattcga taagaagaac ggtgatatta
    30001 tccgctccgc ctgcttcatt ggcacgatca atcagctcag ttactttatg agacaatggg
    30061 ccttcttgat tgatgatcgt tcctatttct tcttcagaca gcatgttcgt caagccatct
    30121 gaacataaca gcagtcgatc ttttagttgc caaagatgag tagaaacatc tacttcgact
    30181 gttccaggca tgccgacaga acgtgttaaa atatttttcc ttggatggtt cactgccatt
    30241 tcttcgctga tttctcctga tttgactagt tcattgacta aagaatgatc ctccgtcaat
    30301 tgcatgattt tatcttcacg aatcaaataa gctctgctat ccccaacatt ggcaatcgtg
    30361 aaccagtcat ctgacagagc ggccgctacg attgttgttc ccataccgtt atattccggc
    30421 tgttcttgtc cacgctgata aatcagcata ttttcttctt gaatcgattg aatgaaccat
    30481 tgggcgattt tttcatcgtc aacagcttct tgtttttccc aagcttcgcc tagattcgtt
    30541 accgccatct gactggcaat gtctccggca cgatgaccac ccatcccgtc tgctaataca
    30601 gcaagcttga gacctgaacg gctggtaaat acactggcat agtcttgatt tgtgttacgt
    30661 cgtcttccta catctgattg atattcaatc tgcatttttt cacctcatta ctttttgcgc
    30721 aaacaacaaa tgaaaaatcc atctgtgtaa aactgatgcg gatacaaagt caacattttc
    30781 tcttcgattg atgattgtag cataggattg gtcaaaacat caatcctctc aaaatcaggg
    30841 tgtctttcca agaaagcaga aacaacgtct tggttttctt cttttaagat cgtacaagtg
    30901 ctataagtca gaataccgga agactttaaa gttggtgcgc agctttctaa aatttccaat
    30961 tggatttttg gtaaattcgc taattcatcc ggctgttttt tataacgaat atctggtttt
    31021 cgccgaagca gcccaagacc tgaacaaggc gcgtctacta aaatccgatc aaactgctct
    31081 gcttcgaact gtttactgac ttctctagca tccatctttt gagcgtagac agcagaagag
    31141 acacctagac gtgctgcatt atcttcaatg agtttgactt tgtgttcgtg tacatctaaa
    31201 gcgaccactt ctccaccgca tgacggatct aaaaaagaag caatatgcgt cgtcttacct
    31261 ccgggtgctg cacaggcatc taagacatga tgttcaggct cgatttgcat agacggcgct
    31321 acaagcatcg aactttcatc ttggatcgtc atgacgccat cgtggaacaa tgagctagaa
    31381 gctaaatgcc cttttttcgc tactataccg tatggactga cttggctttt ttcagctgtg
    31441 atctcttctt cttgcaaaac agtgatggcc tgatctctgg aaagtcggcg tgtatctact
    31501 cgggcactag catggctagg ctgatatagc gataaaccga gcttttcagt ttcttcgtat
    31561 ccgatttgat ccaccagtcg tttcgtcatc catagaggca ggctgatttt gacagaaagt
    31621 ctttcgatag gatcagcaat gccttgcaga tctggtgctc cttgacgctg ataattcctc
    31681 aagacaccat ttacaaattt ccctgtccca ggatttcctt ttacttttgc gatttcgacc
    31741 gcctcgttta aaatagcatg tgcagggact ttatctaaaa attctaattg gtatacagaa
    31801 agataaagaa gcatcttgac ccagtcatcc actttttttg ctttagcgat caatggtgca
    31861 acgtaaaatt ccaatagtcg ctgatggcta atcgtaccat agatcaattc tgtaaataaa
    31921 cccacatctt tttgatttag ctcgctttgt ttgatcatct cattcaacaa aagattcgag
    31981 taggcgccac ccttgttcac tcgctctaat gtcagtaatg ccatatatcg tactgatttt
    32041 ttcatttttt tagggatttt tttcattatt tcacctgctg accaatctga acttgctgac
    32101 cgcttccgtt aaggaaatca tgtacggcct gttttccttt accagctggc tgtaattcat
    32161 tgatggctaa gacggtctgt ttaccgcaag caatccatag attttttttg tctttccgga
    32221 tgatcgtacc aggtgctttg tctgtagttt ctgccaatac ttcaaccgat aataatttcc
    32281 agcgtatgcc ctcataggtc gtgaatgcaa ttggccaagg acgcattccg cgtacttggt
    32341 tgtcaatttc ttctgcagtt ttatgccaat cgattgcttc ttgttcccgt gtgatgtttg
    32401 gcgaaaaagt tgcttctgac tcgtcttgag gtactggttt ttgtccatcc aaaatattgg
    32461 gcaatgtttc tagcaatagc tttcttccta aaaggctaag cttttcaaac atcgtcccta
    32521 catcatcttg tttagtgatt ggtatgcttt cttgcgcata gattccgcct gcatccattt
    32581 ttttgatcat ttccatgatc gttacaccag tttctttctc cccattcatg atggcataat
    32641 gaacaggcgc acctccccga tatttaggaa gtaaagaagc atggacattg attgccccta
    32701 attttggtac ttgcaataac ttttcaggca agaattggcc aaatgccgca gtgacgatca
    32761 catcaggcac caattcaatg atttcttcca tttctggcga accggagatt ttctctggct
    32821 gcagcacacg gataccgtgc ttcagagccg ctttttttac cggtgtcggc gtaattactc
    32881 gtttccgccc aactggacgg tcgggctgtg ttaccactgc ctggatctcg taacctgatt
    32941 ctatcagccc ttcaagaatc ggcacagaaa aagcaggtgt gcccataaat actagcttag
    33001 tcatcaagat gttcctccat atattcttca agttcttctt ccgggatctg ctgtatcatt
    33061 ttctcaatga acaagatacc gtctaagtga tcgatttcat gctggaaggc acgggctaaa
    33121 tagccaaaag ccgtcacttc tatctcttct ccgtctcgat cataataacg cacggtcact
    33181 tcatctgctc gcttgaccgt tccatagaca tgcggaatac tgagacaacc ttctacgtca
    33241 aggctttcac cttttgcttc gatgatttct gggttaatca gttcaaattt ctcgccttca
    33301 tctacttcga tcacagcaat tctcttgttt tgtcctactt gaggagcagc gatccctatt
    33361 ccgtcattcg caatcattgt ctcatataaa ttgtctaata aagcgattgt ctcgtccgtg
    33421 attacatcga ttggctgagc agttcttttt aatttatcat tagggtggat caatatagga
    33481 tagcgcataa tttggccctt tcttaaataa agtttaatgg ttcgttgtca attgccacaa
    33541 aaagaccttg tcgctgttct ttctggcttc cattcaaaat ttcatccaaa acatgaggaa
    33601 gattcggttc atgtttatat ttgatgatta gttgataata atatcggttt ttcactctca
    33661 aaatcatgct gggcgttggc cctaatagca agctttgagg gcttaacact tcttttagct
    33721 gattggcaat ctgaaagatt ttctttgccg ctacttgttc ttcaggatgg ctggcggtta
    33781 ttttcacagt aaagtaataa ggaggatagc ctccacgatg ccgtacatgc atttcctgct
    33841 gataaaactg ctcataattt tgttttttag ccaattctat ggcgtaatgt tgaggattga
    33901 atgtttggat cacgacttcg cctgcttttt ccgctcgccc tgctcgtcct gaaacttggg
    33961 taagcaattg gaatgtatgc tcactagaac gaaaatccgg taaattcagg gaggtatcag
    34021 cattcaacac cccaactaat gtcacttctg gaaagtccaa acctttagca atcatttgtg
    34081 tccctaataa tatatctgct tcttttgcac cgaatttttg taaaatctgt tcgtgtgccc
    34141 cttttcttcg agtggtatct acatccatac gcaaaatcct agcttgcgga taaagttctc
    34201 ggagttcctc ttctactttt tgagtacctg ttccatagta gcggatttta ttgccgccac
    34261 aattaggaca gcgattggga ataggttctt catgtccgca ataatggcag cgcattgaac
    34321 gtgtatccat atgcagtgta agagagatat cgcaattagg acatggaaga acaaatccac
    34381 agtccctaca catcacaaaa gacgaatatc ctcgacgatt gagcagtagg acagtttgtt
    34441 cttttttgtc taatcgattt tgtatttttt cctgcaaatt agcagaaaaa gtagaggtcc
    34501 gatgattctg aaactcttca cgcatatcta cgatctcaat agctggaagc tgtgcagctg
    34561 ctttggctcg gtggtttaac tgcaataact gataaacgcc tttttgagct cgtgctcttg
    34621 attctaaaga gggcgtcgcg cttcctagaa ctattgggca atgatgatat ttactgcgcc
    34681 aaattgctag atctctcgca tgataacgcg gtgtctcatc ttgtttgtaa cttgattcat
    34741 gttcttcatc gataatgatc agcccgatat ttttcagcgg ggcaaaaata gcagatcgtg
    34801 cgcctactac tacttgcgct tcttcccgct caatcttgcg ccattcatca tatttttcac
    34861 cttgggaaag tccgctatgt aagaccgcaa ctgattctcc gaagcgtcct ttgaatcgct
    34921 cgaccatttg cggagtcaat gcgatttcag gcactaacat aatagccgtt ttattttctg
    34981 acaaaacatc agcgattgcc tgaagatata cttctgtttt cccgctgcca gtgattccct
    35041 ccagcaaaaa gacctgatct ttttgctgct gacctgctgt gatgatttgt tcgacagcat
    35101 ttttttgttc tgcattcagt tccaatgccg tcgtttggtc aaatactcgg tctttataag
    35161 ggtcgcgata acgttctgat tctataaact ccagccagcc cttttctttt ccttgattca
    35221 agacagcagt actgattcct ttatttttga aataagcaat tggttcttct tggctcagtt
    35281 cagataaaca caaaagaagt tctttttgtt tcttggcatt ttgccgtaat tttgcccatt
    35341 cttcttcgat ttgcttttca gttaacaaag aacggaccaa acgaatcgtt tttactttat
    35401 ttcgtgtatg gacttcatat ttgatatcta ctaattgctc ttggcgccag cgaatcaatt
    35461 gagaaaggct gccttcttct tctgcttcct tccaggaaat cacttctcgc tgatcaaaca
    35521 atacctgaac ttctggtgtg tctgccaaag gatagatcaa tttatcatat tctgctttca
    35581 tcacactcgg aagcatcgtt tgtagacaag tgattttgaa tgcgtaagta atttttttca
    35641 tataatcagc taatgaaagt aattcttcat tgaccaccgg agcaaggtct agcaaacgaa
    35701 taactgcctt caaagatgga ttagctgatt cttctgattt ttggatagcc ataacaaatc
    35761 cttggacatg acgattgcca ttaccaaaag ggacttctac tcgcatcccg acttgcaaag
    35821 ctgtttccat ttcagaggga accaaataag tgaatggctg atcggtctgc atagtaggta
    35881 cgtccacaat gacttcagct gttaatgtca tggttcgttt cctccttttt ttgtcttttc
    35941 ttatcaagcg acaaaaagtt ccttgcctat tttactagag atcgatggat ttgtcttatc
    36001 ttctgccaaa tcctaacaaa aaattaacag cagataagaa aaaaaagaac tgtgtcaaaa
    36061 agttcagaca cagccttttc taccagacaa taagtggcga atgtttgaaa aggaaacagc
    36121 tgggtttgct tttcttttta gatgttcgct ctatttttct ttattacatt ttttgctctt
    36181 cacggatacg tgattccaat tcgtgttgtt cacgctcttt ttgtgcttga cgttcctctt
    36241 cttccatctt caaacgttca cgttttaatt caggatgtgg atcattgact acatttcctg
    36301 catcgatttc ttctaaagct tgaccgacgc tttttactga atcaaaagaa tctagagtag
    36361 gctgtgcact tgcatccaat tcatgcgcgc gtttacttgc taaaatcact agagaatatt
    36421 ttgaatttac acgatctaat agtgagtcaa ttgaaggttt taacatcata agactacatc
    36481 tcctttaaca ttttaatata tttaccgatt acgcgatcta ctcgaaaatg ttcactggca
    36541 ataatgtttt tgattcgttc aactgctagc ggcacttcat catttacaac agcatagtcg
    36601 taaagagcca tcatttcgat ttcttctttt gcaactttca ttcgtttttc gattacttca
    36661 tctgcatctg ttcctcggcc tacgatccgt gattttaatt ccgctaaatc tggcggagtc
    36721 aaaaagatga atacaccatc gggcactttt tctttgactt gttttgctcc ttggacttca
    36781 atctccaaga aaacatcttt tccttcatcc aatgtcttgt tgacatatga caaaggagtt
    36841 ccataataat tcccgacata ttctgcgtat tccagcatct ctccagcttc gatcattgat
    36901 tcaaactctt ctttgcttcg gaagaaataa tcaacgcctt cgacctcccc ttcacgcatt
    36961 ttacgggtag tcattgagat cgaatactga aaatcattat cctcactctc aaaaattgct
    37021 tttcgtactg tgccttttcc tacaccggat ggaccagaca acacgattaa taatccacgc
    37081 tctgacatga cgacgtcctt tcaactagtc tttttgtact tatcgtttat tttgcacttt
    37141 ttttctgcaa ctttcaaggt tatctttaat tatagctaga aattggtaaa aaagctaccc
    37201 tgtaccaaga ggtcgtaaaa aagctgtcct gcatcaagta ataagaacag ccaaacataa
    37261 acagcttctc atgtttttcg tttggttggg gttattatcg cagatgcaag cttttgaacg
    37321 ccgtgtataa gaggtcgtga agaagccgat tagtttcgag tatgaagaaa aattttgtga
    37381 aaatagtttc tcacattttt gcataaattt ggcttaatac cgagaaaatg gtttttgaac
    37441 atcacttatt caaaataaaa aaagattgtg acacgaattt tgtcacaacc tttaaatgta
    37501 ttcctagagc aaagaagcta agcgaagaaa tccaattctg tttatttatt gtatttcttt
    37561 gttcagcatc cttgctaaaa gcccatattg gacaaagtat tcactacacc catgatctca
    37621 tcttttcgag aacgtgcttt ttctgtcgga tcttctacct cttctgtgtc aattcgaaca
    37681 ataaatttcc gattcatcgt cacgtaagca ccgtcgttga tcaaaataaa cagttcgttc
    37741 tcattatagt ctgcacaggc atctttccat acgtcaaagc cgtttttttc agaagggata
    37801 tcccgtttaa ccacagtttt tccacttaat tcgtggaacg ttacgtcata atatttcatt
    37861 atctctacct ctttcgtcat tcaatatttt gtacttgttc tctgattttt tctaagatcg
    37921 tcttagactg gacgacttga tttttaattt cgattgcatt tgatttggaa ccgatcgtgt
    37981 tgatttcccg attcatttct tggatcagga aatctaattc ccgacctaca ggacattgtg
    38041 cttcgataag ttcatctagt ttttgtacat ggatcgccag acgatccagt tcctcgtgaa
    38101 tatctccgcg ttccaacaag atggctagct ccgtcaacaa ccgttgctca tcgactttcg
    38161 ctccaagaaa ttcctccagc tttttttgat accttatttg aaattccttt tcatagattt
    38221 ctacaaaact atcgagtttt tttagtactt ctttgaattc ttcagtattc tttttcaaca
    38281 ctaaagctaa agcttgacct tctttttctc gactcgcctc aatagaatca agtgcgcttt
    38341 tcaatgtatc tagagctaac tgtgccaatt cttccatgct ctcttccggt ttttcttcta
    38401 ccgttacgaa gttttcgtcc agcacgaagc gttccgccat ccgactaatt gacacttgat
    38461 tttgactacc gtatcgttgt tgaattccgt ctgtcaattg agtgataagt gaatcaatca
    38521 attcccagtt gatgacgatc tgtttttcgt ttttgccaat ataagctaga ttcacaaata
    38581 cttcgattcg accgcggctt aactgctgtt taataagctg acgaaaatcg ttttcaacaa
    38641 agttcaggat tttaggactt cgtatttgaa catctagaaa acgctggttg acacttttta
    38701 tttcaatatc taactgatac gcttttgttt cgcggatggc tttgccaaaa ccagtcatac
    38761 ttttcattcg acagggtcct tcctcattac atattttcta ttgctttttg cagttgttca
    38821 aactcttcgt tcgtcaatgt caggcctttc cccatttttt catggtctgg cgcccaatct
    38881 cgaagatcga attttggcgg gcgaccattc cagctgacta ggttcagttc tttacgccac
    38941 cccttgttgt tttctgacaa tatagcgatt tcttctacga tttcatatga aaattcttgt
    39001 gccatggttg ttcctccttg agataatata aatataggat cagtaatgtg tcaactggtt
    39061 gcttaagcaa ttcatgctgc aaacgatcct tgcgttttcc ttgtagcaga taatcaaaat
    39121 aaacagtttt cgtgcgctca ttttgtattt tttgctggta ttttcgatac cttcgccatt
    39181 cttcagtact taattgacca atttgtgcca ataaatcatc cggcatactt tttatatctt
    39241 gttggcagat catttgaatc atcatgtcta cttgatagat ttcattgggc agtaaaaaat
    39301 gtttttttgc taaaagttct agcatcgcca atccgcatgt ctctggtacg accctgttct
    39361 tatataaatt acgcagtttt tgccaaaatt ttatgctttt gaaaaaagat aattctaact
    39421 gaatttcttt tgttaagtaa gacaaaaatc ctcgccagta tgttcttgca ggtttaacag
    39481 aatgaaatgt ataccagttc ccatagtaat agcctaatcg ttttttcatt tccagatagc
    39541 ctaattgtaa atttaattgg ttcctttggc tatcaaaaag cagaaagctg cctagtgtcc
    39601 acaatgtttg acatttccaa tggataaaaa tgtctggcat acgaattttt ttcgctgggc
    39661 ctggcccttg cacatctacg acaaatgcct ctgttgctcc tttgttaatc gcaatatcaa
    39721 taggtatttt gttgcgatac ccaccgtctg catatttatt ttttccgatc tttcgataag
    39781 ccatagcggg ataaaatgaa gcagaggcga gtatccaatc tggtatttct tctgtttttg
    39841 tctgttggat atcagtcact acttccgcta gttctggcag tttagtagac accgtatata
    39901 aacgtatttt gggattatgt ttgagtaagg acaaatccaa attgtctttg accatctttt
    39961 ccaatggagc agaagaaacc ccgccttcaa ttagtgctgt tttcgtcatt tggcgtcttt
    40021 ctcgatagaa gcgttcacga aggtcttcaa ctgcagccat ttctggtaac gcaagcactt
    40081 gtcttgtcga cagctttttc cataaattca cagctttttt catatctcct tgtaaaatca
    40141 atgcgccgtt caacgcacct accgaggtac ctgtgataat ctcaaagttg atatcatgtt
    40201 ccttcagcgc ctgccacaca ccaatctgat aagcaccgtg cgctcctcct ccacctaaaa
    40261 caagtgctgt atgatagacc agctttttat ttaattgtgt ggttccttga tagccgtttt
    40321 tatgaaaaat acgcaaccat tcttctgata gaggagattg aaataaaaaa caacagtttt
    40381 ctttaataaa aaaacgagcg caagcttcca tataccgaaa aaattttttc cattcaaaag
    40441 tttcaccttc aatcaacaga ttattaaaaa taacatctcc tctactttta gcaatagtaa
    40501 aatagacggt atattttttc gatactgctt taaaagcaca ttgctttttt gcagaacgcc
    40561 aaaaagcgtc tctactttct tgatgacttt gaaaaaatgg aaggggttgt tttgcttgct
    40621 ggataggcga atacagcgac attttctgtg aaaagtaaac tttttttatc tccatcgcgc
    40681 tctccttttt cttcaagtat accagtacgt cataaaaagg tcatgagtct cgtattcgac
    40741 tttgtagatt ccataagaaa acaattcata aaacctcctt taaataaaaa aatacaaaaa
    40801 tcacaaaaat taattgaaaa tgagagtgtt ttcaatgaaa acgtgatttt gtaatggaat
    40861 tttaaaataa aagaaacaag cttttttaat gcaaatgttg taaaataaaa gagattaaga
    40921 ggaaataaga ggaggaacat gatgactgag tcagtttatg tacatattga taccacaagt
    40981 aatgctgttt taacaaaagg gctgactgct actgactttg caaacagtat cgtgcatttt
    41041 cctcaaaact tgctcttgct tgatccatct gcttctgctg gagaatatga atcccataca
    41101 gggctgaaag taatccgtgg tacagaaaat attcagcgtt tcttttcctc ttctcgtaac
    41161 aggcgtggat tcgatgaatt gaagtggatc gactttactg atttaacgat gctgaaagaa
    41221 ttgacacctt tagaaatttc agaattatta tattttggtc atatgaaaac acatcttcat
    41281 tcgccgtttt tttataaact acagaataat tttgtatact tcgatcttaa tgatcagctg
    41341 aatcggattt attatcgcta tttagatgaa ttttatagga tttttgctag taaattaagt
    41401 tcgatcttgt cagataagat caatgtaaaa aaatcttttt tcaaaaaaga aacacctgtt
    41461 gaaaaaatca gtgtggaatt attgaaagaa atgcgtaatt tgatgcagga agggatcatt
    41521 ttttcttttg atcaagttgg tttgatcgat ggtaaatatc gtatcccaat ctatgtgatt
    41581 gaagattata tttgga
//
LOCUS       DAPBCS010000023        41377 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPBCS010000023 DAPBCS010000000
VERSION     DAPBCS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.023"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1015..1305)
                     /locus_tag="RD521_001405"
     CDS             complement(1015..1305)
                     /locus_tag="RD521_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771149.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2205)
                     /locus_tag="RD521_001406"
     CDS             complement(1513..2205)
                     /locus_tag="RD521_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7771150.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            2600..3223
                     /locus_tag="RD521_001407"
     CDS             2600..3223
                     /locus_tag="RD521_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7771151.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(3307..4029)
                     /locus_tag="RD521_001408"
     CDS             complement(3307..4029)
                     /locus_tag="RD521_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDT7771152.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(4019..4756)
                     /locus_tag="RD521_001409"
     CDS             complement(4019..4756)
                     /locus_tag="RD521_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771153.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLPGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(4768..5760)
                     /locus_tag="RD521_001410"
     CDS             complement(4768..5760)
                     /locus_tag="RD521_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7771154.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(5775..6518)
                     /locus_tag="RD521_001411"
     CDS             complement(5775..6518)
                     /locus_tag="RD521_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771155.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(6592..6696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6759..8081)
                     /locus_tag="RD521_001412"
     CDS             complement(6759..8081)
                     /locus_tag="RD521_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7771156.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(8068..9033)
                     /locus_tag="RD521_001413"
     CDS             complement(8068..9033)
                     /locus_tag="RD521_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771157.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(9030..10568)
                     /locus_tag="RD521_001414"
     CDS             complement(9030..10568)
                     /locus_tag="RD521_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDT7771158.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(10984..11271)
                     /locus_tag="RD521_001415"
     CDS             complement(10984..11271)
                     /locus_tag="RD521_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771159.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            11405..12073
                     /locus_tag="RD521_001416"
     CDS             11405..12073
                     /locus_tag="RD521_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7771160.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(12364..13104)
                     /locus_tag="RD521_001417"
     CDS             complement(12364..13104)
                     /locus_tag="RD521_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771161.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(13234..14181)
                     /locus_tag="RD521_001418"
     CDS             complement(13234..14181)
                     /locus_tag="RD521_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771162.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(14310..15074)
                     /locus_tag="RD521_001419"
     CDS             complement(14310..15074)
                     /locus_tag="RD521_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7771163.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(15078..15467)
                     /locus_tag="RD521_001420"
     CDS             complement(15078..15467)
                     /locus_tag="RD521_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771164.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            15602..16144
                     /locus_tag="RD521_001421"
     CDS             15602..16144
                     /locus_tag="RD521_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDT7771165.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            16292..16924
                     /locus_tag="RD521_001422"
     CDS             16292..16924
                     /locus_tag="RD521_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771166.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(17058..17183)
                     /locus_tag="RD521_001423"
     CDS             complement(17058..17183)
                     /locus_tag="RD521_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771167.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(17317..18210)
                     /locus_tag="RD521_001424"
     CDS             complement(17317..18210)
                     /locus_tag="RD521_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDT7771168.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(18392..19318)
                     /locus_tag="RD521_001425"
     CDS             complement(18392..19318)
                     /locus_tag="RD521_001425"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDT7771169.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(19403..20164)
                     /gene="pflA"
                     /locus_tag="RD521_001426"
     CDS             complement(19403..20164)
                     /gene="pflA"
                     /locus_tag="RD521_001426"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDT7771170.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(20408..22639)
                     /gene="pflB"
                     /locus_tag="RD521_001427"
     CDS             complement(20408..22639)
                     /gene="pflB"
                     /locus_tag="RD521_001427"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDT7771171.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(22916..25366)
                     /gene="parC"
                     /locus_tag="RD521_001428"
     CDS             complement(22916..25366)
                     /gene="parC"
                     /locus_tag="RD521_001428"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDT7771172.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(25378..27423)
                     /gene="parE"
                     /locus_tag="RD521_001429"
     CDS             complement(25378..27423)
                     /gene="parE"
                     /locus_tag="RD521_001429"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDT7771173.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(27589..28023)
                     /locus_tag="RD521_001430"
     CDS             complement(27589..28023)
                     /locus_tag="RD521_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDT7771174.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            28150..28788
                     /gene="plsY"
                     /locus_tag="RD521_001431"
     CDS             28150..28788
                     /gene="plsY"
                     /locus_tag="RD521_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDT7771175.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(28920..29795)
                     /locus_tag="RD521_001432"
     CDS             complement(28920..29795)
                     /locus_tag="RD521_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDT7771176.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(29877..30668)
                     /gene="codY"
                     /locus_tag="RD521_001433"
     CDS             complement(29877..30668)
                     /gene="codY"
                     /locus_tag="RD521_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDT7771177.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(30686..32086)
                     /gene="hslU"
                     /locus_tag="RD521_001434"
     CDS             complement(30686..32086)
                     /gene="hslU"
                     /locus_tag="RD521_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDT7771178.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(32100..32648)
                     /gene="hslV"
                     /locus_tag="RD521_001435"
     CDS             complement(32100..32648)
                     /gene="hslV"
                     /locus_tag="RD521_001435"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDT7771179.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(32685..33590)
                     /gene="xerC"
                     /locus_tag="RD521_001436"
     CDS             complement(32685..33590)
                     /gene="xerC"
                     /locus_tag="RD521_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDT7771180.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(33788..35164)
                     /gene="trmFO"
                     /locus_tag="RD521_001437"
     CDS             complement(33788..35164)
                     /gene="trmFO"
                     /locus_tag="RD521_001437"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDT7771181.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(35133..37211)
                     /gene="topA"
                     /locus_tag="RD521_001438"
     CDS             complement(35133..37211)
                     /gene="topA"
                     /locus_tag="RD521_001438"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDT7771182.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(37333..38190)
                     /gene="dprA"
                     /locus_tag="RD521_001439"
     CDS             complement(37333..38190)
                     /gene="dprA"
                     /locus_tag="RD521_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDT7771183.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(38246..39013)
                     /locus_tag="RD521_001440"
     CDS             complement(38246..39013)
                     /locus_tag="RD521_001440"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDT7771184.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDVRSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(39006..39866)
                     /gene="ylqF"
                     /locus_tag="RD521_001441"
     CDS             complement(39006..39866)
                     /gene="ylqF"
                     /locus_tag="RD521_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDT7771185.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            40126..40524
                     /locus_tag="RD521_001442"
     CDS             40126..40524
                     /locus_tag="RD521_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7771186.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            40803..41303
                     /locus_tag="RD521_001443"
     CDS             40803..41303
                     /locus_tag="RD521_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDT7771187.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
BASE COUNT        11366 a         8641 c         7026 g        14344 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2461 attactgtat ttattacaaa aaacgcggat ttgtcataaa ttgtcatgct tcttttacct
     2521 atgaaatatt ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa
     2581 cgaataaagg agatttttca tgacatcact taaaacttta ctttttggaa caactttggc
     2641 tgccggcgct gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca
     2701 atcaggtgat actctatcaa cgatttctca aaaatacgtt ggtgataact ccttgatcaa
     2761 tgccattgca gaatcaaatt caatttctga tattaacctg atttactcag gacaacaatt
     2821 aacaatccca acagaaggct ctgcacaagc agctgctgaa cctcaagcag ccgttcaaga
     2881 agcaccagtc caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaaaacaagc
     2941 agcgcctgtt gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc
     3001 aagcacaagc tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc
     3061 aacgaacgga cgttacatcg gacgttacca attagattct tcttacttaa atggtgacta
     3121 ctctgctgca aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg
     3181 ggaagcggcg aaagcattct gggaagcaaa cggttggtac taagattcat ttattaatca
     3241 aacagtttac ttttatacaa agaaagccaa aaagaggtgc gacactagtt gtgtcacatc
     3301 tcttttttat ttacctaatc taatgaaacg ttccaagttt tgataagtat tttttttaac
     3361 ttcttcaact gatagctctt tgatctcagc gattgtcttt aactgttctt caagcagtga
     3421 gacataatct ttttctgaaa aaatcccccc taaagcccac tctgctgcat gtagtccatc
     3481 cgtttcaata agtaagtggt cgattgggac tgctttagct agttgccaga ctgctggatt
     3541 ctttttcaaa tcaagaccaa tgctgaaata acagcctaaa tcaatatagt cttgttgata
     3601 gtccaaagaa gaataccaat ggatcaaata gtgattaggg taagcttgga tataatttaa
     3661 gatctctttt tcgcatcctt ttgtgtgcag tacaacaggt ttctcattta tggctgcaaa
     3721 agcaagctgt cgctcaaata cttttttttg tgtagtcatc ggtacattcg tccaaatatt
     3781 atctaaccct atttcaccga tgatccttgc ttttttcaaa aaaggctcaa cttgttcaaa
     3841 cgtatagcta tctgctttcc aaggatggat accgtaactt aaggcttgag tcttacttgc
     3901 tgcaagctgt ctgttttcct tccattcttc tggcgaatca caattgatga tcgtcaaaat
     3961 cgtgtgttcc tgctgaagga gtgctaaact ctgactccca tctaaatgac aatgagcatc
     4021 acttaacatc taattcacct gccaataact gataaatcgt ttgcttcaac tctgattgct
     4081 gcagtagcca ttcacgtgac cgtcgttttt caggcacttg aatctcagca atcacatgtg
     4141 ccggacgggc ggaaagcaca agaatcctgt ctgataaata aattgcttct tcgatatcat
     4201 gcgtcacaag taaagtagtc cgctttgctt ttttaggcag cttcaacatc cagtcttgca
     4261 tctgccctct ggtcatagca tctaacgctc caaatggttc atcaagaagc aagtaagaag
     4321 aaggattcat gattgttcgt aacaaggccg ttcgctgctt catcccgcca ggtaattgtt
     4381 ccggcaaaaa atgttcatag ccgtttaacc caaagatatc cagataatcc cttacttgtt
     4441 cttcattgat tgttagatga ttgatttttt gatataacgt aacgttttga tagacgctca
     4501 gccaaggaag taacatatct tcttgcggca tataactaaa tgctgtatgg agaccagtta
     4561 ctttttgatc gtctactttg atcgttccag aagtcatagg ctggactcct gttaaagttt
     4621 tcagcaatgt tgacttaccg gctccgcttg gcgcgacaat cgatgtaaag gtatctttag
     4681 ggatagtgaa atggatgtct tctaatattt tcaggttttc aatgactaca ttcaagtggc
     4741 tgatttggat tgacatatct atcactccta ttttataaac tgattagtga atagttcttg
     4801 cgaaggtaca tcttgcttga tcaatccgtt ttcctgcata aaagctgtat aattgcgcca
     4861 aactttttct tccatcaatc cccaattctc aggatcatcc gtataatttt ttgacaagaa
     4921 tgcttgtgat tcttctaaaa acgtccgatc attttctttt gcatattttt gcaacagttt
     4981 agcactgtca tttggatcct taatagcttc ctgatagcct tttttcgtgg catccataaa
     5041 agactgtacc aattctggat cacttttgat cagttgatca ttcgtaataa ttaccggagt
     5101 atagtaatct aaccgttcat ctaattcttt caatgggata tagtttagtt caatacccgc
     5161 ttcttttgct ttgatcatat cccaaccttc aaagtaccat tggatatctg aggattttcc
     5221 taagctttct ggcccttctc cattgcttcc taccattgtc agttttgaaa agtccccgcc
     5281 gtctttttcc atcacagctt ttaagacagc ttcttcactt ggactttgcc aacctgcata
     5341 tgtttttcct tcaaaatctt ttggtgaatg gatgttactg tctgataggg tggcgaatcc
     5401 agaagtatta tgtttgatca ctgtagcaat tgctttgact ggtatattct gtccatctgc
     5461 gtgagcataa gtgacatctt cttgataact tacaccaaat tgtgctttgt ccgcacctac
     5521 tagaccaata cttgtcgaat tatctcctgg ttcgatgatt tggacattta aaccagcctc
     5581 tttataatag cctttttctt ttgctaggta gatgccagta tgattagtat tcggtacata
     5641 atccaaaaca agtgtcactt ttttcaaatc ctttatttct tgtttgctac ttgtttcttt
     5701 tgcagtgcca caagcggata aaagcaacag tagcggtaat aaaagccata attttttcat
     5761 ttgatcccct tctcttaaaa aatataaagc atacgataat accctttttt caataatgaa
     5821 caagcgccat tcagcaataa actcaataga atgacacaaa tgatcgcagc aaaaacttta
     5881 tcgatctggt agctgttttt tacgcgtatc atgtaatacc ctaagcctgc ttgagcgctt
     5941 agccattctc caacgactgc tccgctgaca caataagtag cagccacttt taaaccggaa
     6001 aagaagccgg ataaacccgc tggtatttta tatattcgat aaatcttccc tgtatctgcc
     6061 cccatcgttt tcaaaaagat gatttgctct tttgatactt tcctcaatgt atccgaaaaa
     6121 gaaactacga tcggaaaata gcttatcaag acaacaagga tcacttttgg aagcataccg
     6181 aaaccaaacc atagggtaag taatggccct aacaccatga ttggcaacat ttgtgaaatc
     6241 actagtaacg gataaaaaga catttctaaa aacttccaat aatccattgc caaggcagta
     6301 ataaaagaaa gaacgctagc tattaccaaa ccaagcactg cttcatttaa tgtcgtgaca
     6361 gaatgggaaa gtaatacttg tgcatctgcc gctaatgcag aaaatacagc agcaggtccg
     6421 ggtaaaataa aggcaggtat tttccaaact atgacaacaa tctgccaaat aattaaaatg
     6481 aatgccatac tgactgctgg ataaagattt ttcttcattt tgttacctcc tttcttctaa
     6541 tatgaataac aaaaaacgct tgaaatgggc acgacatagt aacccgcttc aagcgtctgc
     6601 tatgtttctc ccttcaccag tattaactga caggttcaat gggtctaagt tcacaactta
     6661 ctctcatgtc gtctacgaca tccccagaaa caggtattca ttttttaaag tgtagcacaa
     6721 gaaggaaaaa gcaacaagcg ctttagttat catgctgatt atttcaataa ataggagaga
     6781 tcacttgcca atgtcggata ccccatgata tagctatcca actctttttt agtcaatttt
     6841 tgattgatga cgagtgtcaa atcatcgatc aattcgtctg cttgctcgct aatcactgca
     6901 gcaccaatca aataattttg ctggtcaaag gtcaattcag ccttagcaac tggttcattg
     6961 acacgatgat aggtgaacca actggtaaga tccatctcta ccacttgatc tgacgaagag
     7021 gcgtgacttt ttgtaactcc tacttcagca agtttaggac tggcgtagac gatcgtaggt
     7081 ataatcggat atttgatagg ttcgcttgtt gcatccgtta tccgcttagc tacataattt
     7141 ccttcaaaag tggcgactgg tgtcaatttt ggggttttcc ttgaaacaat atctccacac
     7201 gcaaaaattt ttggattgct cgtttgcaga taatcattga ctgcaattcc gtgtttatca
     7261 aagacgatat tcgcttgctc aagtgctaaa gattctgtat tcggctgtct cccagtcgca
     7321 caaaaaatca tatctgctac taattccgtc tcttttccga ctaaacgata gcgagtacct
     7381 tctgaaatga tcttttgggt ttcaacacca aaagcaaact gaatgccaga tgcttccatt
     7441 tgatggacgg cttcctctac caaagacgcc tcaaattctt ttaatggccg ttgattatga
     7501 tggacaatgg ttactttgct ccctgcagca ttggcaatcg tagctagctc aaaagcaata
     7561 taaccaccac cgataaaaat gatttcttta ggcagtacag gcaatgataa aaagtctgca
     7621 ctagttttca agtattcctt tccctccact ggtaagatcg taggtctttg cccggttgcc
     7681 aaaactaagt aatccgcatg gaagacttct tcgttgactt caatagatga atcatccaaa
     7741 aaacgagcgg ttcctgaaag atgatcgatt tcagcttctg ctaattgttt ttttcggctt
     7801 tctgggactg gatccgtaaa tgttcgtttg aatttttgaa gttcttccca gttggcagtt
     7861 gggatctcgt taaaaccttt tccactcaat tgttttaccc gattacgtgc ctctactgca
     7921 cttaacaaga cttttttagg atcacatcca cggttaggac aagtcccgcc ccatagatct
     7981 tcttcgacca ctagtactgt tttacctgct tctttcaatc cataagcagc acttaagccg
     8041 ctcacacctg aaccgatgat gattgcatca tatgttttca tttccttcct cctctcgact
     8101 taaaaaagca aagatttctt tacttgtcag cgacccaata cttctttgct tttctactat
     8161 cacaatgctt ggatacaccg aaagaagcga atatacttct ttcataggcg tatcaaagca
     8221 aacttgttga cgagcatttt ctggtatttc atttaagaaa tatccattga cgactaaatc
     8281 ttctactcgc cactctttcg tctgtttcgg ttcatcaaag aaatgccgaa cgtagtcgtt
     8341 tttaggattc atcaataact gctctggtgt gtcacattgg ataatttctc catccttcat
     8401 aaatgcaata cgatcaccta gcttgattgc ttcttccata ttgtgcgtaa cgaaaacgat
     8461 ggtcgtgccc aattcttcgt gtaaggacaa aaccaattct tgtaatgagt ttcttgaaat
     8521 cggatctaat gcgctaaatg gctcgtccat caaaatcaca tctgggcttg cagcaatagc
     8581 tcggatgatc ccgatccgtt gctgttctcc tccagacaac tctctaggca tacgatctcg
     8641 ataaatatca ggagataagc caactttttg aagaagttca tctacacggt ctgctctttt
     8701 ctgcttttcc caacctaaca tttcagggat gacttcaata ttttgcttga ctgtcatggt
     8761 cgggaacaaa gcgatttgct gcagcacata tcccatattc caccgcattt tctgcagatt
     8821 atattcattt aacgattttc ctttgaagta gatgtctcct gctgatggtt tagataatcc
     8881 attgatcatt ttcaaagtag ttgtttttcc actaccggaa ggaccaacca aaacaaagat
     8941 ttctcctttt ttgattgcca gatttaggtc atcgatgaca tactttccat catacttttt
     9001 ttgcacatgt ttgaactcga ttaatttagt cactgatctt cctcctttac taatccttca
     9061 tttttcagga agattcgtgc tacttctgtc ggctttttct tttcgacatt gacttggtaa
     9121 ttcatatcaa tcatttgatc ctctgttatt tttccactta atttattcag tgctttaacg
     9181 atttcaggat gttctttagc aaaatctttt ttcatcagcg gtgcgccttg ataagctggg
     9241 aataaccctc gatcatcttc tagagtcact agatcgtact ccttgagctg gctatctgtg
     9301 gaataggcat ccacaatatt gacatctcca ttattgattg cttggtaacg caaacttggc
     9361 tccatgcttt gtacacttga aaaattcaag ccatatgctt cttggattcc tttatatcca
     9421 tctgaacgat caataaattc cagtgtgaat cccgctttga tctgatcaga gatagctgcc
     9481 aaatcggaaa tctttttaag ttgatgttcg ttcgcaaaag atctcttgac tgccaaggca
     9541 tacgtatttt ggtaagccat tggttttaat aatgtcatat cgaattgctt attcaataat
     9601 tcttctgctt cgtcatatgt tttttctttt gtcaaatcag cacttttcat tgcttcaggt
     9661 accttcacca agctttctag gacagtacct gtgaactcag gatagatatc gatttgacct
     9721 tgatccagtg cattgaacaa aaaactcgtc ttgccaaaat tcggttttaa ttcaacttct
     9781 gtttttgtgt tgtcttcaat caattcttta tacatttgga tcaggatttc aggttccgct
     9841 cctaattttc cagcaatcgt gatggtttca tttttaaaca aggcagactg atataaagaa
     9901 acgccgccaa ataaacacac cactgctgct aaagtaatga tggaatatcg aggtttctta
     9961 tgctgcaacc agcgaatcag tccgctgaat ataattgcaa gcaaggcaga actgatcgca
    10021 ccaatcaatg tcaaaacagg tgtatttcgg tcaataccta acaaaataaa tgtccctaat
    10081 ccacctgctc cgatcaatgc tgccaaagta gccgtaccga tgattaaaac taaggcagtt
    10141 cggatacctg agataatgac aggtaatgcg atgggaagct ctactttcag caagcgtctc
    10201 attctggaca taccaaatgc taccgctgct tcttcaatag acgggtcaat ttcagctaga
    10261 ccaatatacg tgttttggaa aatcggaagc aaagcgtaaa taacaagtgc aatcaatgct
    10321 ggaacagttc cgatccctac aaatgggata agtaaaccta ataaggcaag tgaaggaatc
    10381 gtttggagca cacccgtgat ctgcaataag acttctgcca tttttttatg attggctgcc
    10441 caaatcgcta aaggaatagc aatcagtgct gcaatcagca aagaaatgag agaaatggac
    10501 agatgttgaa atgcagcttc cagcaactcg ccttttcttt cacttaatgt ttgtataaaa
    10561 ttattcatgc gaccttcctt tctgttattc tttaagtata aacatttctt tcctttttcc
    10621 caactaactt gtcttgcctt aaccttacag cacgaacaaa aaaaagaaaa aaatacctct
    10681 aaatatgctg acaaaaaaag aatccaagca acaaagtcgg atcaactttt tggttctcgt
    10741 tttttcacta cgaataccat aaaaatctat acgaaattgt cacttggaat cagctatatc
    10801 ttttttacac acacaacttt tattttaata aaaagaaaca gccaataaag tagaaatctc
    10861 gtgggggaga acacctttat tggcctgttc aacgaatatt atactgtatc atgacacttt
    10921 tttgccaaaa atgtaaaaaa tggttgtttt gacatcataa acgtcttgtt ttcttatttt
    10981 tacttatatc accactttat tttctatctc tttcactcca taaagaggaa tgagttcttc
    11041 ttttgactga taataaacag ctttctcacg gttcttcatc aaaaaaagaa agatgtcttt
    11101 catagcaagt ggtgctttgc cgtgttcaaa taaaaagata aattgatttg ctttatttat
    11161 ctggaccatt gtcacaggca ccagtttttg ttcttgagcc aaaaaaactc cttgatactc
    11221 tgtttttttg ggtgattttg taagaattgt caataagtct gctgctcgca tttttccact
    11281 ccttatttga taaataaaaa gaaacagcca aaaataattt tttttcattt tcctcttgaa
    11341 gtaagagtat aagtcagtat aataaagaag ttgctgaaaa ttttcaaaag aaaagaggct
    11401 agaaatgaaa aaattaaatt tatttacaga attgtgcgga atcattttag gcgcaggaat
    11461 ctacggatta gctgtgacag gaatcaatat tccatctaag ctggcagatg gtggggttac
    11521 tggtattgct ttgcttttgc ataatctttt tggattcgct ccttctgtca cttcactgct
    11581 ttttaattta cctttattgc ttatctcatt gtttcttttt ggaaaacacg cttttctccg
    11641 gacgatcgtc ggaacatttg cgctagtttt ctttttacat ttatgggaga gccttcatat
    11701 acattttgct gtagatagtc tactgatcaa tagtttgatg acaggtattc tttctggcat
    11761 tggttgcggt ctcgtctttc gattcggcgg aagcacaggc ggaacagata tcatttatca
    11821 agccattgaa aaatattttc atattaagat tggcaaaagt cttttcttga ttacttttgg
    11881 tattttagtt gtctccttgc tttatttgga tctcacacat tttctgtata cattacttag
    11941 ctgttctatt ctatcttata cattgaataa agtgaagtat ctggaaattg gatctgtttt
    12001 gccccaattg gaagcaaaac agactacaga aacatttgaa ccacttgatc ctcttgaaca
    12061 gttagagcac tgattcttat ttttcactac tttatttaat ttacagaaat atcattatgt
    12121 aaactaaaat gatattattc agtaaaatct tctttataaa aaaataaact atttttgcaa
    12181 acgatctgcg aaaatagttt gtttttttat tttgccatta ttttcttact tattgggaga
    12241 aaaaattcgc ttaaactcaa aaaaatttga ggatacgata taaactttcg gatagagtat
    12301 aaaataagga aacgccgcag cgtttccttt ccaactgtag ccgaatataa atttgtatat
    12361 cacttattta aaatagctat tttctcccaa agagtttcct tgatcatcga tcaatccttg
    12421 taatgagcgt tctaagatac catggctttt tttcgatttt ttttcttctg actgctttgt
    12481 ttcctctaac gtttttgttc gattgaatgt tcgtacagac ggctcaccat ttcttttttc
    12541 ttgatacggc gtcggttctg tatcgaacaa cagataagag tcatccgttt ttttcatcga
    12601 ttcgattaaa tgatcctcag aaattttgct ttctctaggg tctgggataa tcgatgcggg
    12661 tacatattta ggaacaaaat aagaacgctc ccgcaccttc gtgtcaacag atgcgtttcg
    12721 tgtagtatgc ttggactctg tccttgtttc actttgtctg acagaacgag aggaatcacc
    12781 aaaaagtttt tttcgtcctg tcgatgtgat ttctgattgg taatggccgg tataatccgg
    12841 taattttcct ttatgctttt ccaaattttc cttacgcgga gctgacttca atccatttgt
    12901 tgcacgacgc ttgcgacgga ttttctcggt gttcgtgttc gtgaaatgaa attctttcat
    12961 ctgttcaaac ggggactgat tctgttcagt caaaagatct tcttgtccgt caaataatac
    13021 tcgcttcttg tcgttggtta attttacgcc taactcatca tcgtacgcag gaaaacggaa
    13081 atgttttcgt ctgatttctt tcattcaaac catccttctc aaaaatggaa cttttctcaa
    13141 taatagcaca gattcattgt tttcagtagc aatgcgctga ttattttaag aaaatagcga
    13201 tgaatttcat aaatatatat atagtaataa gacttaattc gttgtctctc tgataactag
    13261 ttcaggaaaa aaacgacgat aaccttgact cgcccctttt tcttctattt tttttaacaa
    13321 catttctcca catacttcac ccatacgtat aatatcttgc ttgatcgttg tcaatttagg
    13381 tgaactgatt tggtctaaaa ataccccatc aaatccaatc acaccaaatt cttcgggcaa
    13441 gtgtcctcca gcatttataa tccctcttac tgctcctagt gctagccgat ctgaactaca
    13501 tacaaaacaa gttttatctg taaaatctgc ccagttttct gtcatatatc tttctacttc
    13561 tgtcgaatga tttggaaacc gcaagatttt actaggaaga tcaacttcct tcatcacaga
    13621 taaataacca gtttcccttg aaatttcaaa aggctcgtcc acttcgatac cgatataaat
    13681 aatcgtttga taatctcttt ctaaggcata ttgcgttgct tttgctgtac catattcatt
    13741 atcgctgtct acataatcat atccttcaga attttcccca aacaagacaa ctggtttttc
    13801 caaacggtcg atccaatcga agtctttttt tctgacacct gtgatgatgt atccatcaca
    13861 agaaccgacg tcgaaggcat catttgtaac taattggaga gaataatgcg ctttaccgat
    13921 ccgtttagcg attcccatca gcaaattcat gtaatagggc tcagttgtgt cgatttcttc
    13981 cagtataaat aatttgatga tctgcgaccg attcgtgaca agtgcttttg cagcaatatt
    14041 tggacggtaa tccagttcag ccattgctga gaaaactaac tctttcaatt cgtcagtcac
    14101 ctgttccgga tgattgatga ctcgagagac cgtcattttt gaaacattgg cacgttttgc
    14161 aacatctgct aaagtgggca tgctctcttc ctttctaaca tgttgtcatt cttactatca
    14221 attataccaa attttcctaa cagagtttat tatagcccaa aataaaaaac ggaaaatcct
    14281 ctatcgtcat gagtgatttt ccgtttcaat tatttatact tttttatcaa attttttgct
    14341 aaaaaagtgg aagcttcttc cattagtggt atttgtacca tatgtcgctg tgtttttgag
    14401 gaacgaaaaa caatttcttt tccgtaagac ttatgccgat ttttttctac aaatcgttcc
    14461 gtttgtatat aagggatttt ttcgtcagct tgtccatgcc aaaagaacag aggacgtcca
    14521 gctaactttt ccggctgcaa tgacaaatca tagctcttaa cccatgaaat caaatcaaaa
    14581 tagtcttttg gtacgacata attatattcc gatgcgcgcc ttacgatttc ttttccatat
    14641 tccaaaggtg caggggaacc catcaaacac acgcccgcta ctatatgagg atgtttagcc
    14701 aacaaggcgc atgttgtcat tccccccatc gaaaccccgc caactgcaag ctgatcatgt
    14761 agaagacctc ttttttctaa atgcttgata agtgtgtcaa attcaaacag atttccgtag
    14821 atactatccc aaaatgtata ggatggaata gaagaaaccg gctgtttgcg ctccccatga
    14881 ttcatcgcat ctggcaggat cacacgaatg ccctgctgcg caatttttct tccttgcgtc
    14941 aaaactaact ccttacttgt ctgccatccg tgataataga tgacaacggg aatacgttcc
    15001 tctcttaatt ctttcagaac aacttctaac actggaatat gatggacaga acgtttcaat
    15061 ataattggat tcaattgtta ctcacctatt ttcattaaat ttcctgatcg ccatagcaac
    15121 catgctccaa cagttgtctg gaacaggttc agtgtaccaa ataatataaa tgcaggactg
    15181 aatggctcgg gaaataagca cagagaacaa agtcccacca ctcctaatgc tagcgaaagg
    15241 agtggataaa tagaaaaatt caaaaaactg gcaacgctac cgaaaatatt accaataaac
    15301 agtatcaaaa agaaaaaaac gcttaaaatt aaaccatctg tcagcgacat ctgaaaaaca
    15361 atgttctcta cctcaattgg ctgttgaatc gaatagacac ctaagtatat actgagaagg
    15421 agacttagca cgtggctggc acaaaacaca cgattgaccg ttttcatttt aatcacctaa
    15481 cctatcttta taattctatt ataatcattt tatcattttt ttgtatgtat aaagtttcaa
    15541 tttttcttca cttacgattt tttttgatat tgttgttata taaatgaaag gagttttaca
    15601 aatgattgat ccaaaaatgt tggggatgat cttcctcatt aattttgctt atatcactct
    15661 gaacacgtta cgtttcatgt tgacaatgaa aggctatcga ctgattgctc cacttgtcag
    15721 tatggtcgag atcacgattt atatcgtcgg cttatccctt gttttagatc gtttagataa
    15781 tccactgaat ttatttgtct atgcgttagg ttacgcagta ggtatcagtg tcggaatttt
    15841 gattgaagac aaattggcat taggatacat catggtcaca acgattttgc cagcaaatag
    15901 cgcagaagat aaaagtttgc cgggtatact gagagaacac ggttatggtg tgacacagac
    15961 ttctgcaatg gggctggaag gtgagcggct agttctcgaa atactttctc ctagaaaaag
    16021 cgaacgtgaa ctgtatcgcc tgatcaaaga aatcgaagag cgtgctttta tcatttctta
    16081 tgagccaaaa tatatttcag gtggtttctg gactaaaaaa gtgagaaaac gacaaggaaa
    16141 ataactcaca aacttaaaaa ctgtctaata tctaaattaa attttaaatg ttagacagtt
    16201 tttatttatt attttgatta aaaaacatcg acatatactc atcaatttgt tattatactt
    16261 tatatactta tcaatttaat ttggagatat tatggaaatt caacgtagaa ccactaattt
    16321 tttacattta ggctcttttt ttgcaacttt gacgatctta ttttttctat gttattcata
    16381 ccttaagacc tcacatgcgc cttctagtgc attaggctgg ttgcttttgg atcattctga
    16441 tatcacagaa gccgaagaaa atccgttttt tttgattttg tcttctcttt gcggcttact
    16501 gttttctgtt tatttcggtg cctatttttt tcatcgacat cctggtacgt taaacgaagc
    16561 ttcaagcgta ttgattgcat ttaaaacgtt ttctttttta gcaattcttt tttattcgtt
    16621 taatcacaat acgcttttct tctttatcat gaatggttta gtcgtgattt tatccatttt
    16681 tacttatttt attttttcac tcatttttag ccaattgaaa tgtccgctct tctttcgatt
    16741 agttcctgtc acctgtttta tttacagctt gttttccttt tttgtccttg ctttaatgcc
    16801 actgaccaac ccttcgacga tccaagtcgc aggaaatctg ttggatatgc tcttcatcgt
    16861 cagtctaggt gtatttatgt ggcaaagaaa aaacaaacaa aataaaaagc attttgaagc
    16921 ctgactatta cgacttcata aatgcttttt tagttttaaa cacttcaatc taaaagaata
    16981 acccaagatt ggataaatta ttaatgactc gccattcgtt tcttagtgat tctggattat
    17041 caaatggact tcctaatcta atcagctagt tcttcccata aaatgccata cttgactgaa
    17101 tcataatatt gattatttaa aaatctaact tttcttatca ctacttcctt tgtcattcca
    17161 catttctcac catttttttg cattcctttg tttccagacc atgtagtgaa tctaataggt
    17221 ggtagatagg caaaatactc aaataattct tttaaccata aaggaagaga aaggctcctc
    17281 ctgcaagagt cagtataatt tttgcttact gctctcttag gttttactca ctaaaactgt
    17341 tccatattct ttgatataag aaaatgcatg cggtataaat ttcttgctca gctcagaaaa
    17401 gatttgtcgt ttaagaaagg ccatttcctc cattgtcaat gaaggatcca atgttggtaa
    17461 ttcaatctca aaattgtttt tttcgacaat aggtaattct aatgtgaatg attcttcttc
    17521 ctttatagta ttcgtcacaa gtagagactg atggaaaata ctaggccggc gatttttata
    17581 tttattctta cgtatcctgt caacgataac ttctatttga tcaaaaaagt acgattcatt
    17641 tacgtctcca ataagttcgg ctaattcttt gaatgccttc gttacaggaa gctgattctt
    17701 ctgtacaaaa aagtaattag ccaaagtaag cgggaagtgt tctatagagg aaagtgtgat
    17761 aaaacggtac ttctcatttc ctgcagttgt cctcagcaat tctgtctctc ggctgtttct
    17821 tactccataa aaatacgctt ctttccgttc atcttcgaaa caatacgccc aaataacttc
    17881 tcccatcgct aatttttgtt catcaacaga gagtctcttc aattgttcag ctatggtttc
    17941 agcagccgaa gtggcttgtt gcaagtaatc tttaggatca aatattggaa agttcaaatg
    18001 atatcttcgt tcttccctct tgattaatcc tgcatcgatc aataaatcaa gaaaatggga
    18061 aaatttattt tgcgaaaatt cttttttcaa ttctcttaga atcgtttctt gttctcgatg
    18121 ttcatataga taatcggtca atgaagaaaa aagcggatca tcggtcagtt tttgataaaa
    18181 gttttcctta ttttttgaat aatagttcat atatgttctc ttttctatcc gttttatttt
    18241 acttaaaagg cttttacgta aaaaggagga agaacaagag tcataattga ctgttattct
    18301 cctccatttc ttcggccttt agtatgttct atgtttcttt ttatatcaat cgcttatcca
    18361 ttcattttga atgcttcata ctaccatttg attagctaaa tacagccgtc aattgaggta
    18421 cgacttgttt tttacgtgaa acgacacctt taagcaaagc acggttgttg gtcaaagttg
    18481 tttggaaagc ttcttctact ttttcttttc ctttaccagc aacaagtaat tctgaatcac
    18541 tgtctaagat gtttgtgaca actaatacga acagatcgta gccatttgct ttcatttcat
    18601 cttccattgc tgcttctagc tctgcttgtc gatccatcac ttcattaagg tccactgtat
    18661 tgacttgagc gatacgaacg ttggaactgc ccattgggaa actttttgca tccaaatcaa
    18721 gaagaaccgc agctgattta tcacttagat ttgttcctgc tttcaacatt tcaagtccat
    18781 aagtgtttag atcgacttca gcgattgctg caagttcttt tgcagctgct tcatcttcag
    18841 gcgtacatgt tggtgatttg aacaacaatg tatctgaaat gatagctgag agcatgattc
    18901 ctgcgatttc tttaggaatt gttacttggt tttctttata tagttttaaa ataatcgtac
    18961 ttgtacatcc aactggttct gcacgatagt acaatggatt agctgtttca aagtttgcaa
    19021 tacgatggtg gtctactaca gctaaaatat ttacttcgct gatatctgaa atactttgtt
    19081 ggaattcatt atgatccaca agcatgacat ctgcattttc tgctttagct gactctacta
    19141 ctcgtggtgc agatagctga aagtggtcta atgcgtattg tgtttcttca ttaggtgtac
    19201 ctagagcaac tgcttctgtt tctttaccta attcattttg caagtgtgaa aacgcaatgg
    19261 ctgccccgat tgcatctgta tctggatttt ggtgaccaaa aactaaaatt tttgacatgt
    19321 cattcgctcc tttgtgattt ctcctattta ataatagcaa aaaaacggaa agaagcaata
    19381 cttatcttct ttccgatgcg acctatcgcg ttaaatattt tgtatactct tctgtatgaa
    19441 gcaatcgttt agcattttcg acacgttctt ttgtaggtgg ttcgatccct tctaatggat
    19501 atttcaaacc aagtgtttcc cacttgtatt ttcccatcgt atgataagga agaatctcta
    19561 ctctgtctac atttgacaag gtttgaataa agttgtctag tcggatcaaa tattcatcat
    19621 aatcacttcg ctcaggaact agtacatggc gaatccaaac cggtttgttg atttttgaca
    19681 aatattgagc catttctaaa atattttcat tgtcccaatg cgttagtttt ttatgctctt
    19741 cattatcgat gtgtttgatg tcaaataaca gtaaatctgt atacttcatc aattcttgaa
    19801 aacgtgaaaa gaaaggttct tcatacgtaa aaggttttcc gcatgtatcc aatgtagtat
    19861 gcatcttccg ctcttttgct tttttaaata gttcgatcaa aaaatcgatc tgcaatagag
    19921 gttctccacc actaacagta ataccacctt tatctcccca gtaaggtctg aagcgttctg
    19981 ctttatctaa aagttcatct gctgtgtatt ctttccctcc gccaatattc caagtatctg
    20041 gattgtgaca aaattggcaa cgcatgcggc atccttgcat aaacacgaca aagcgcagtc
    20101 caggtccgtc tactgaaccg aaagtttcga tagaatgaac atagccgatt gttttttctt
    20161 ccatttaagt tcacatcttt tctttgattt cattttaagt acaagttagc tgtcgaagcg
    20221 aagctttctg aacaaaattt ttcgcaattg ccgcttcgtt atgtgtaaaa tttacaggga
    20281 cggaacatac aagtcctgcc cctgtaaatc gtttctttca aataagcata ctgattattc
    20341 gattttgatg cgatggctat tcaacgtcct ttcgacgcta agtcgaaaaa cttttttcac
    20401 gaacttttta cattctgtcg tgtgaagttc tcatcaagac atctaattgt tgttctcttg
    20461 tcaagtcacg gaatttaact gcataaccag atacccggat cgtcaagttc ggatatttca
    20521 ttggattttc cattgcatca cgcaataaat cattactgaa tacattgaca ttcaaatgat
    20581 aagctccttt tgagaagtat ccatctaaga cattacgcaa gttttcgatt cttgtatctt
    20641 gttcttttcc aagacctgaa ggattgattg tttgggtatt tgaaattcca tccaatgcac
    20701 agccgtaatt cattttagct gttgagttca ggctagctaa taacccactt tgttccgcat
    20761 tgtaacttgg atttgctcct ggcgcaagtg gttttccagc ttcacggcca tcaggagtat
    20821 ttcctgtatt tttaccgtaa acaacatttg aagtaatcgt caataatgaa gttgttggtt
    20881 ttgagttgcg gtaagtttta tgacgtttga tttgtcccat gaagtattcc aagatccatg
    20941 aaactaattc atctgcacgg tcatcatcat ttccgtattt agggaagtcg ccatccactt
    21001 caaaatcaac tgctattcct ttttcattac ggattgcttt tacttttgca tatttgatcg
    21061 ctgacaagct gtctgccgca tgagagatcc cagcaatacc tgtagcaaat gtgcgttgca
    21121 agtcagaatc catcaaagcc agctgagctg cttcataaga atatttatca tgcatataat
    21181 gaatgatgtt caacgtattg acatatagtt ctgctaacca atccatcata ttcatgaagt
    21241 tatgtttcac ttcttcaaag tctagtattt ctgatgtgat tggacgatat tctggtccaa
    21301 cttgggcgcc agtcacttca tcgactccgc cattgatggc atataatagt gttttagcca
    21361 agttagcacg agcgccaaag aattgcatgt cttttccgac aactgttcct gatacacagc
    21421 aagcaatcgc acaatcatca gaaccccatt gttcacgaag ctgattatca ttttcgaatt
    21481 gaatcgaaga actttcgata gcgattctgc tagcatatgt tctgaatcct tctggaaggt
    21541 gttcagaata caaaacagtc aagtttggtt ctggtgaagg tcccatattt gttaatgtat
    21601 gaagcagacg gaaatcattc tttgtaacta atgaacgacc atctaatccc atacctgcaa
    21661 tggataaagt agcccagata gggaatcctg agaataattg gttatattca ggtgtacgcg
    21721 cgaatttgac catacgtaat ttcattacta agtggtcgat caattcttgt gcttcttttt
    21781 ctgtgattac tccattatcc aaatcacgtt ggatatagat atcaaggaat gctgagatac
    21841 gtccgattga catcgcagca ccattttgtg atttgattgc tgctaaatat ccgaagtata
    21901 gccattggac agcttctttt gcatttgcag ctggtttaga aatatcatag ccatatttcg
    21961 cagccatttc tttcatttgc gctaatgcac gatattgttc agtgatttct tcacgtaaac
    22021 gaatcacttc ttcactcatt gtatgataac cagtcatatc atgatctttt ttcttttgtt
    22081 ccatcaaata gtcgattcca tacaatgcca cacgacgata gtcaccgatg atacgtccgc
    22141 gaccgtaagc gtctggaagc cctgtaatga ttttgttttt acgagcagca cgcatttcag
    22201 cagtataagc gtcaaataca ccttgattat gtgttttgcg ccagtcagtg aaaatatgtg
    22261 tcaattcatc gtttggtgta tagccattgc tttttagcgc attgttagcc atattgattc
    22321 cgccgaacgg catgaatgct tgtttcaaag gtttgtctgt ctgtagaccg acgatttttt
    22381 ccaaatcttt atccaaatag cctgcatcgt gtgaagtaat agttgatacg atttctgtgt
    22441 ccatatcata gacaccgtta cgctcgaatt ggatatcatt taacttttgc aattcatccc
    22501 ataatttttc tgttgcttct gttgggcctt ccaaaaagct gtcatcaccg cgatattcag
    22561 tgtagttatt ttgaataaag tctctagtgt ctacgccttc tttccatttg gtacctttaa
    22621 atccattcca ttgctccatt gtatggacct cctaaatttc tgttttatgt aacagttttt
    22681 acgtgattag tataacacag caccctatct ttgcaagggt tttcttgttt tttatttaac
    22741 aatttttatg ttttatttac atatatttaa atcaacgttg acataccaac atttaagcat
    22801 tgtgaacact ttcatctttt tttgcaaacg tataaggatt tattaaaaat gaatgaaaaa
    22861 gagacgccac ctgtattaat acagatggcg tctcttcatt aaaatactac aacagttacg
    22921 actcgatgaa aatctcagga gcatcgataa cttcgatgac ttctccacct tttttctcat
    22981 ccatcgcaaa tgatccattc gatgttcgat cacctatagg gaaatcagtt gggtcaatcg
    23041 ttaactgctg ccctttttga ttcatcaagg ttatttcttt tgatcttcct tccgacatat
    23101 agataatacg gtgcggattt tttttcagct cacgcatgac cgtcactccc cgcttcgcac
    23161 gtgaagtttg gctgatttct tgagcaagca tgcgtttaac agctcctcgc tgagtaacga
    23221 ttacgatcgg cgtatcacct tctgctaaca caagcagccc gttgataatc ctatctccat
    23281 ctttcaaatt catagacttc actccagctg cctttgctcc aacaacggga acatctgtta
    23341 aaggatagcg caaacccatt ccttgatgcg ttactaagaa aacatctaag cctggctgat
    23401 cttctttcag atacacagca atcagttcat cttcttccgc ctttagcttc atacagctga
    23461 gcggacggct cttataggtg cgccatggtt cgaactctgt cattcgtgtt tgcttgatca
    23521 atccattctt gctgatgaat acaaatgttt tctctgcttg aattttttga tatgggaaaa
    23581 cagctaaaat cgattcgtct acagacagat tggagattgt ttgagaaatg tgctcaccag
    23641 catctttcca tttcaaatcg ggtagttcat ggacagggcg gtagatcaca tttgctttat
    23701 ttgtgatcag aagaacatgg tccaatgtac tcaattcacc tgagtatagc aagtagtcgc
    23761 cttctcgcat cccgatttct tctggtttgg aagcactata agaacgaagg cttgttcgct
    23821 tcacatagcc ttctcgcgtc acactgacaa ttacgtcttc ttgtgccact aagacttgcg
    23881 tatcaatttt gatttcttcg atttcgtctt cgattgtcgt tagacgagcg gaagaataat
    23941 tttttttcac ttctcgcaat tcttttttca taacagagaa aagctctttg tcatttgaaa
    24001 ggattttatt caactctgtg atttgcgcaa tcaagctttc ggattccttt tgaagctctg
    24061 tgatatccgt gttcgtcaaa cggtataatt gcaatgtgac aattgcttct gcttgttctt
    24121 ctgtaaattg aaagacgtcg actaaattct ttttggcgtc ttttttgtct ttactttcac
    24181 gaatcgttgc gattacttca tccagaatcg ataaagcttt catcaatcct tctacgatat
    24241 gctgtctttt ttgagctttg gctaaatcga attgactacg ttttgttatg acttgctttc
    24301 gatgctcgat ataacttctt aaaatgtctt tcagtccaac ttgatgcgga gtcatatggt
    24361 cgatagcgac catattgaaa ttgtaattga tctgtagctc agtgtttttg aacaaataat
    24421 tcaaaattcc ttgagcattg gcgtcttttt tcaattcgac gacaatttgc agacctgttc
    24481 tatcactttc atctcgtact tcagctattc catcgatttt cttattcaaa cggatctcat
    24541 ccattttttt caccaaggtc gctttattta cttcatatgg gatttctgtg atcacgattt
    24601 gctgtttatt acccttcatt gcctctatct ttgttttcga acgaagaata acttttcctc
    24661 gtcctgtttc ataagctttt ttgatttcat cttttccttg caagatccct cctgttggaa
    24721 agtcaggtcc aggaatatat tccatcaatt tttcaaggct cgcttgtgga tgatcgatca
    24781 tatagattgt gccatcgatc acttcagcta agttatgagt cgggatttct gttgcatatc
    24841 cagcagatat acctgtcgat ccattgacga gaaggtttgg gtatcgagct ggaaggactg
    24901 ttggttcttt ttctgtatca tcaaaattcc acacgagatc gactgtttct ttctcgatat
    24961 ctgctagcat ctctccactt aacttggata atctggcttc agtataacgc atcgcagcag
    25021 gtggatcacc gtccatactc ccgttattcc cgtgcatttc gatcagtact tcccgcaact
    25081 tccagtcttg gctcatgcgg accatcgctt cataaatact gatgtcacca tgaggatgat
    25141 aattccccat gatatttccg acagattttg ctgatttacg gaatcctttg tcatacgtat
    25201 tgccatcttt gttcatggca aataatatcc ggcgttgcac tggcttcaat ccatcacgga
    25261 tatcaggcag tgcacgttct tggataatat atttggaata acgtccgaag cggtcaccca
    25321 tcacttcttc taaagttaat tcttgtactt cttgtcgatt ttccatcagg tgacctccta
    25381 ttctacttca aataactgat tgttttcatt tttatctgct cgttcttcat ctaataaatc
    25441 attagaaact gaaggagaga tttcagtatc ctctttttta tctaagatac tgccatcttc
    25501 ttcgagcgta aattgcacgt gattttcgat ccattttctt cgaggttcta ctttgtcacc
    25561 catcagtgtg gttactcgac gttcagcttg cgcagcatca tcgatacgga cgcggatcaa
    25621 cgtacgggaa gtcggatcca tcgttgtttc ccacaattgt tccgcattca tctctccaag
    25681 tcctttatat cgctgcagca tatatccttt gccgactttt ttaatcattg ctgccaattc
    25741 gtcatctgtc cacgcatatt caatgacttg cttctttcct tgtcctttgg atactttata
    25801 taaaggcggt aacgcgatat atactttccc agcttcaatc agcggtttca tataacgata
    25861 aaagaaagta agcagtaata cttgaatatg ggcgccgtct gtatccgcat cggtcatgat
    25921 gattactttg tcgtaattac agtcttcgat agaaaactca ggaccgactc ctgcaccaat
    25981 cgtatagatc atcgtattga tttcttcatt tttcaagata tcttgcattt tagctttttc
    26041 ggtattgatg acttttcctc gtaaaggtag aattgcttgg aactttctgt ctcttccttg
    26101 tttagcagat ccgccagcag aatccccttc gactaaatac aattcatttt tcttagggtt
    26161 tcgggattgt gcgggtgtca attttcctga aagaagagat tcgccttttt tacgcttctt
    26221 cccatttcgg ctttcttctc tggctttacg tgctgcttct ctcgcttctc tggctttgat
    26281 tgctttacgg accaacatct ggctcatttc gctattttcc tgcaaataaa agcccatttg
    26341 ttcactgatg acgttatcta ctacggtacg agcaactggc gtaccaagtt tttcttttgt
    26401 ttgcccttca aattgcagaa gattttcagg tacacggata gaaagtacag cagctaaccc
    26461 ttctctgaaa tcactacctt ccagattctt gtcccgttct ttcagcaaac caacttttct
    26521 cgcatattca ttataagatt tcgtcatcgc cgctttcata ccagcttcat gggttccccc
    26581 atcttttgtc cgaacgttat tgacaaaaga taaaacattt tctgaataac cgtcattgta
    26641 ttgataagcg acctctactt cgataccttc tttttctcct gaaaaataaa caactggtgt
    26701 caatgtatct ttttcttcat tcagataatc aacaaattct ttgattcctt cttcgtaatg
    26761 aaaaatttcc ttgactggtt cttctccacg aagatcagaa agagagattt taacgccttt
    26821 tagaagaaag gcagattctc ttaaccgttc ggctaaaatt tcatatgaaa atttcgttgt
    26881 actgaatatt gtatcatctg gtaaaaaatg gacagtcgtc ccgtttgctt tttttgtttt
    26941 tcctatcttt ttcaaagtac cgtcaggttt accgccgttt ttaaatttct gctgatactc
    27001 tacgccatct ctaacgatcg ttacaattag ccatttcgat aaggcattta ccacactggc
    27061 tcctacaccg tgcagtccac cagaagtttt gtaccctcct tgaccaaact ttccgccagc
    27121 atgcagcaca gtaaaaatca cttcaactgt tggtattccg gaagcatgca ttcctacagg
    27181 catcccacgt ccgctatctg caacagttat actattgtct tcatggatgg tcacatcaat
    27241 ctcactgcca taacctgata atgcttcgtc aactgcatta tcgacgattt catagactaa
    27301 atgatgcaag cctcggctgt cagtggatcc aatgtacatc cctggtcttt ttctaactgc
    27361 ctctaatcct tccaatactt ggatggacga gtcattgtat tcgttgttta ctttttttgc
    27421 caaggcaaac gctccttcaa taatactttg cggcaaaacc gcaccacgat tatcatacta
    27481 gaaaaggtcg tgaaaaaaaa gagggtagac gagaaatcaa ttttaattat ctcctaaccg
    27541 ctttttcgac attcccctct atcgatcatc tcttctttgt ggtcattttt atttttggtt
    27601 ttctttcaac ttaccaagtt caatcttaat acaacgatcc attacgatat ctgtacgtcc
    27661 tgcttttctt agcatctcag ctgcttcttt gttttcaatc cctaattgtg cccagaacac
    27721 tttagcatca gttttcaaaa aatcacctgc tatttcaggt aaaaattcac tccgtctaaa
    27781 tacatctacg atatcgattg gatcatcgat ttctgtcaaa ttcgcataga ctttctcgcc
    27841 tagaatttct tcaccagcca ataatggatt tactggtatg attttatatc cattgtcttg
    27901 caatacctgt gcaaccatgt aacttgttcg ttctggttta ttgctcaagc ctacgacggc
    27961 aatatttttt gcttcttcca aatagttgaa aatcgtttct tgtgttggat tttttacaga
    28021 catttctgcc accccttgct tttcttttta ttccatctat tatacttgta tctttcattt
    28081 tttcaattgt tcctgttacc tattcatgct aaaataaagg atagttttca acttgaaaga
    28141 gggtaaaaaa tgaaattgat cttattactt attttagcat atcttttagg ttctattcct
    28201 tccggtgtct ggattggcaa attgtttttc aaaaaagata tccgacaaca cgggagcggt
    28261 aatactggaa cgaccaatac atttcgtgta ttaggcaaaa cagccgggat tgttgtcctg
    28321 cttatggata ttttaaaagg tacattggct acttgtttgc cgatgatctt ccacctttct
    28381 gggattaacc ctctttggtt tggggtatgc gccattcttg gccatacatt tccgattttt
    28441 gctgggttca aaggaggaaa agctgttgcg acaagtgcag gaatgctttt aggatttaat
    28501 cctgtatttt ttgtttattc ttgtatcatc tttatcgtta gtttattttg tacaagtatg
    28561 gtgagtttga gcagtatgat cagtgcagta ttgatcactt tatctacgat cgtccttcct
    28621 tatgtagctc ctgtgatttt ggctaagccc aattggttac tgacgatcat tgccttttta
    28681 gtttcttcct ttatctttta tcggcataaa gacaatatca agcgtatcag aaaccatact
    28741 gaaagtcgaa tcccgtttgg tttgaatagc agcaagaaaa aatcctgata ctttttcatt
    28801 ccaaagagat aaaacgggag tgtgacaaat cgatgtcaca ctcccttatt acttacagga
    28861 tagctttttt acacaccttg gtaaacggtg ttccctgcta acttctcggt cacaacctct
    28921 tacttcactg tgatggaata ttttgtcttg aagatttcat gggcacctag ttgattgatc
    28981 cctacttttt ccatcaagtt tccactactg ttcaccgtgt cagcgattcc gcaccacggt
    29041 tcgatacaaa cgaaaggcgc ttcttttgga taaggagacc agatccctac ataaggcata
    29101 tctttatagc ttaaagttac gctatgggaa gatttttcac ttcgaatggc gtacgcattt
    29161 agaccccttg tttcaaaaac caaggcatct tgctcaaaaa gttcacgatt caaagccaga
    29221 ttcgtatttg tctgacctaa cgtcttttga tccatatcga tgaatgggcc tttcaatggt
    29281 aggcgtaatc ttgatttcaa tggagaaaat gccagataga aatcttcaaa tgacaatcct
    29341 tcttcaagag gtacattgaa agcaggatgt ccaccgatag aaaagaacat ttctttttca
    29401 ccggtatttg ccacttgata atgaaccgtg atgccgtctc ctcctaattc atagctgatc
    29461 actaattcaa aatcaaatgg atacttttct aatgtctccg gcgtactcct taagatgaaa
    29521 ctagccattt cttgtccatg ttcaatgact tcaaaatcca tatcccgagc aaatccgtgt
    29581 tgccccattt gatatgtttt cccttgataa acatactgat catcttttaa acgtccaaca
    29641 aaagggaata aaaccggtgc atgtctgccc caatacgctg gatcaccttg ccagatatac
    29701 tctaaaccat gttcttttga tttcagactg atcaattccg caccgtgttc agcgatttcg
    29761 gcgatcagct ggtcattttg aataataaca gtcattgcca ttcctccaac ttcgacttac
    29821 tttttaaaat gggaaggctg gaacaaacat ttctatctgc cccagtcccc ttacttctaa
    29881 taactgtgtt ttgctaactc gtctttaaaa cgggaattca atacttttaa ataagttccc
    29941 ttcatgccta acgaacgaga ttcaatgatt ccagctgatt ccaattttcg taaagcattg
    30001 acgatcacag aacgagtgat gccgatacta tcggcaatat tagaagcagt cagacgacct
    30061 tcatctccat ccaatgcttc aaagatagct tgtacagcct tcaattcgct ataagataat
    30121 gtgttgattg ccatttggac agccgttgca ctccgtacat ctgcttcgat gcttcgagac
    30181 ttctgataaa ggatctgcat accgacaacc gtagctccat attctgctaa gactagatcg
    30241 tcatctgcaa acgattgttc catacgagat aagataatcg tacccaaacg ttcgccagct
    30301 ccaaagatcg gaacgacagt cgtcagacca aatggatatt tctctcttga ctctaccgga
    30361 aatgcagtaa tatcactagt aattgggata ttagcttcag ttttcatcaa ctgtgccact
    30421 gtatcagtat aagattcagg aaattgtttc tcaacaaaca ttgttttgat tcgttcattg
    30481 ttcacatcgt gacgttcatt ataacctagt aatactccgt cacttcctat gatataggtg
    30541 ttgctgtcaa gaataccacc tagtgtgaca gccattttat cgtaaggcaa atctgcttgc
    30601 atgtcaaaag tattcttttg ctgcaaaagt gtgtttatct tgcgggtttt cgttagcaag
    30661 tctgtcattg tcgtcctccg attttttata aaatatagcg acttaaatct tcatttgccg
    30721 caattgagcc aagtttgtca tccacatatg cttctgtgat cgttatttct cccatttgca
    30781 tatctggtgc ttcaaataat agatcttcta gtagtttttc caaaatggta tgcagtctgc
    30841 gtgcgccgat attatctgtg tcacggttga catcgaaagc gatttgagcg atccgttcaa
    30901 ttgcttcttt tgtaaacgta actgacacat tttctgtacc gatcaacgcg acgtattgtt
    30961 ttactaaagc attatttggt tcggtcagaa tcttcacaaa atcttcagct gtcaaatcat
    31021 ctaattctac ccgtatcggg aagcgtcctt gaagttctgg aattagatca cttggtttag
    31081 acaaatggaa agctcctgaa gcaatgaata ggacgtgatc tgtttggatg atcccgtatt
    31141 ttgtgtttac ttgtgaacct tcgacaattg gcaatatgtc tctttggacg ccttctctgg
    31201 atacttctcc agattgctgt gatttagaag tgatcttatc gatttcatcg ataaaaataa
    31261 ttccactttc ttccgctaat ttgattgcct cactatggat atcagcatcc ttgactaatt
    31321 ttgctgattc ttccttgatc aataactcta aagcttcttt gactgttacc gtccgttcca
    31381 cttttttctt cggtgtcaaa gcacctagag tttcatttag gtcgatcccc atttgctcca
    31441 atccattgtt catcattggt gcaggtttct tttggtcttc aacttcaatg gttacttcac
    31501 gactatccag taaacccttt tgtagttgtt ccaagactgt acggcgattg attcgaatat
    31561 cttctgtcac ttcttcttgc ggttcagcgg tattttgctg actagcatta aaaagattca
    31621 tcatttgttc aaattgatta gtggattgtt tctgttcttt tttgatacca ggtaccaaga
    31681 tttttaccaa acgtttttct gcattttttt tagcacttgt gtatacacgg ctgtactgct
    31741 ctttttctac gatttggatc gcattttcta ctaaatcccg aaccattgat tctacatctc
    31801 ttccgacata accgacttca gtaaattttg ttgcttcgac tttgacaaat ggtgcattga
    31861 cgatttttgc caaacgtcgt gcgatttctg ttttaccgac ccctgtaggt ccgatcatca
    31921 gcatattttt tggtgtaata tcttgttgca ttttttcatc taactgcaaa cggcgatagc
    31981 ggttacgcaa agcaacggca acagattttt ttgctgcgtg ctgtcctacg atatattcat
    32041 ctaattcact tacgatttgt cttggtgttt gttttaattc atacatatct actcacctct
    32101 tacagttctt ctacgataat attatgatta gtaaagatac agatatctgc ggcaatattt
    32161 aacgcatttt ctgcaatttc tttcgcactc atgtcgtttt gagcatagtt tttcattgct
    32221 cgggctgcag ccaacgcaaa attccctcct gatccgatag ctaagatacc atcatctggt
    32281 gtgataactt ctcctgtacc tgaaacaaga agcatttctt tatcattcat tacgatcagc
    32341 atcgcttcaa gtttttgcat agattgctgt gtccgccatt cttgagctaa ttcgacagct
    32401 gcgcgttgta gattcccgtt atattcgttt agtttgcctt caaatttttc ttctaaagta
    32461 aacgcatctg caacacttcc ggcgaaacct acaacaactt ctccattgta gatacgtcgt
    32521 actttctttg cagtgccttt catgacaaca gattcaccca ttgttacttg gccatctcca
    32581 gccatagcaa atttcccgtc tttttcgacg gcacagatag tggttgaatg aaatggtgat
    32641 tcgaccatgt ttgtttcctc ctaaatttgt tcttttcctt atttttacgc tcttggatgg
    32701 aaagaacgat agtttttttg taaactttct tttgttacat gggcatagat ctgggtagtc
    32761 gataaattgg catgtcctag caattcttgg actgtccgca aatctgcccc attatttaat
    32821 aaatgagtag caaatgtatg gcgcagcatg tgaggatgga tatcactatc caaactgctt
    32881 tttttgatca tttgattcaa gacatattca attccagtag gcgtgattgg ttttccatga
    32941 tgattcacaa aaacgatatc atgctcttcg tgatattttg tcatcaatac ctggcggcct
    33001 tccctgaaat attcttgcag agcatcttga gcaaaagaac ctagaggagc atatctctct
    33061 ttgttccctt taccatggat caacatgacc tctgaatcaa aatcaatgtc tgataccgac
    33121 aaactgctac actcacttaa acggatgcct gacccgtaaa ggacttccag taaagcgcgg
    33181 ttcctcaaat ccaaaggact atctcctgct acgctgtcaa aaagtgcttg catttcattt
    33241 tcataaaaaa aacgtggcag tctcaaattt ttctttttga gatgaacgta agaaaatgga
    33301 ttttctttta tcacttcttc tttgagaaga tactgataaa acgaacgtag gctggctatc
    33361 ttgcgactga tagaatttct gctatagcta ggacgatgat cagctaatgc actcaaatag
    33421 acacgaaccg taaaatattc cgccttcagc agatcagcgt ctccagattc ctccaaaaaa
    33481 tgtttgaaat ggagaatgtc ttcttcatat gcttctttcg ttttttctga atatcctctt
    33541 tcgacaatca aataacgcaa aaatttttgt tcccacgtgg cttctgtcat tttctcacct
    33601 tccttttgtc ccagagaata atttagcaca gcgtttataa aaagacaaat aaattgtcag
    33661 aatttaacca atatttctaa gtttgttcac aattggcgca attcctcaca ttttgtttga
    33721 gaaattctta agaaaaacgc aagaaaaaga gcgagactat ccgtctcacc ctcttcatgg
    33781 agtttcctta ctcatttggg attgcagttt tttctaattc tgtttttatt gcctcattag
    33841 cttttaaagc tcgttgtgca attgcttcat atcttgcttt tttgtctcga atgcgctctg
    33901 gcagttcagg aaacagacca tagttggcat tcatcggttg gaaatgtttt ccttccgcat
    33961 gagtaatgta gtaagccata cttccaatag ctgtttcttg tggaaactcg actggttcca
    34021 tttcttttgc taagcgtgcg gcgttgattc cagcaagtag tccgctcgct gcactttcaa
    34081 catagccttc tacaccagtc atctgacctg caaagaacag atcttcgcgt ttcgttgatt
    34141 ggtaagtcgg tttcagcaat tctggcgaat tcataaagct gtttcggtgc atcacaccat
    34201 agcgaacgaa ttcagcattt tctaacccag gaatcatccg gaaaacacgt ttttgctcgc
    34261 cccatttcaa gtgagtctga aagcccacga tgttgtataa agaagctgca gcattgtctt
    34321 gacgcagctg gataacagca taaggacgtt tgccagtttt aggatcttcc aatccaactg
    34381 gtttcatagg tccgaaaagc atcgttttga atcctcgctg tgccattact tcaattggca
    34441 tacatccttc aaaatatttt tctttctcaa aagatttaag ttctgccact tcggcattga
    34501 ttagtgcctc atggaaagct ttgaattcct cttctgtcat cggacagttc aagtaagctg
    34561 cttctccttt gtcatagcgg gacttcaaat atactttatc catatcgatc gtagctttgt
    34621 ctacgattgg tgcagctgca tcatagaaat aaaaaccatc tgaaccattg aatgctttga
    34681 tgctttctgc taatggatga gaagtcaaag gtcctgttgc tactacggtg attccttccg
    34741 gtatttcagt aatttcttcg tttatcactg ttactaatgg atgctctttg acttttcggg
    34801 taattgtttc agaaaaagaa tcgcggtcaa cagctaaggc accaccagct ggcactgctg
    34861 tttcatctgc cgaagtgatt actaccgaat tcaatcggcg catttcttct ttcaataacc
    34921 ctactgcatt agccaaactg tttcctctta atgaattgga acatactagt tcagcaaaat
    34981 tacttgtgtg atgcgcctca gtcgatttga ctggacgcat ttcatataaa cggacaggga
    35041 ctcctgcttg tgcgatctgc caagcagctt cacttccggc aagccctgct ccaacaacat
    35101 ttaccatttt caatgaaaga acctcttttc tattattttt ggatgtcttc ttcgtaatct
    35161 ccatttacac agactacttg tttaccgcct ttgactttct tctcgaccag atatccgtca
    35221 cattttggac aatttctgcc cactggttta tcccaagaag tgaattcaca ttctgggaaa
    35281 cgggaacaac catagaatag tctgtttttc ttagattttc gttcaatgac ttgtccttca
    35341 tgacatactg ggcaagtcac accgatttct tttacgattg gtttcgtatt ccgacaatca
    35401 ggaaaattac tgcaagcata aaacttccca tatttcccta attttatcac catcggatgt
    35461 ccacagacat cacaatcgaa tcctgctggt tcatctttga tttgaatttt ttcgattttt
    35521 tcttccgcat tcgtcagttc tttttcaaac ggtttataga aacgatcaac cactttgacc
    35581 cacgcttccg tgccttcttc aattttatcc aaatctcctt ccatttctgc tgtgaaatgg
    35641 atatcgacga tttgagggaa gaattcacag attaatgaat tgacaatttc tcctagttca
    35701 gtcggctcaa atcttttttg tgtcaatttt acatagtagc gtctttggat cgtatcaagt
    35761 gttggtgcat aagtggaagg tcgccctacc ccgttttctt ctagtgcacg aatcaacgta
    35821 gcctcactaa aacgtgctgg tggttgagta aaatgttgtt ttggttcaat atcaacagat
    35881 tggactacat cgccttcttc aagttctgga agaatatttt ctttctcttc tttgccgtca
    35941 tctctacctt ccacatacac ttgcatgaat cctttgaatt tgatttttga accgttcgca
    36001 atgaagatga caccattttg ttctaatgtc actttcattg tatctaaaat agctggtgtc
    36061 atctgactag ccacaaaacg tgaccagatc aatgtataaa gtttcaattg atctttatct
    36121 aaatattgtt tgagttcatc tggtgtacga agcacactcg ttggacgcac agcttcatgg
    36181 gcatcttgtg cgccttgagc atttttgatc tttcggtgat catgtctaga aaattctttt
    36241 ccatacgtat cttcaataaa tgctgcggcc tctgcttttg ctgtatcggc aatccgggta
    36301 gaatcagtac gcatgtatgt aatcaagccg actgttcctt gtttacccaa agtgattcct
    36361 tcatataatt gctgtgctac catcatggtt tttcttgttc gaaagtttaa cttacgggca
    36421 gcttcttgtt gcaggctact tgtagtgaaa ggattcgcag ggtttctttt gcgctctttc
    36481 ttttctactt ttttaacttc gaagtctgtt ccatcaattc tagcagtgac ttcttttaca
    36541 ctttgtgcat ctggtaattt ctttttctta ccgtcgattc cccagaaatt tgctttgaat
    36601 ttcttgcgag cttttttaaa attaccgtcg atgctccaat attcttctgg aacaaattct
    36661 cggatctctt tttcacggtc aataataatt tttaaagcga cagattggac acgtcctgca
    36721 cttaaccctt tcttgacctt acgccagaga ataggactga tcgaataacc aacgattctg
    36781 tctaaggtac gacgagcttg ctgtgcatct actaaatcta catcgatcgt tcttggttcc
    36841 ttaaaagctg ctttgactgc gtcttttgtg atttcattga agaccacccg atttttttct
    36901 ttcagatcca acccaagtag gtacgctaaa tgccaagcaa tcgcttctcc ttctctatcc
    36961 gggtcacttg ccaagtaaac tttttgagct ttttttgctg cggctttcag gcttttgatg
    37021 acatcgcctt ttccgcgtat agaaatataa tggggttcat aattgttttc aaaatcgata
    37081 cccattttac ttttcggtaa atctcttata tggccaacac tggccattac tttatagttt
    37141 cgtccaagat atttctcaat tgtcttggct tttgcaggtg attctacaat cactaaatat
    37201 ttatacgcca ttactgaatg gactcctttc cattgtcgtg ttcctactat taaaagagga
    37261 ttcatacaaa tcgtagctaa tcatatccat tcagaaaacg tttgtcaaga aaagcaaatg
    37321 aaaagtatgg tgttattctt taaattctgc caagatatct tcacccgaaa ccgtgcaaac
    37381 tgcaccgtct tggatcaaat gatgacatcc ggtagaatgt tctgttaaaa tatttcctgg
    37441 aacagaaaaa actgttcttc caagatccaa tgcttgctgt gctgttatca gagagccgct
    37501 ttttttcttg gcttcaaaga cacatgttcc tttagacaaa gctgcgataa tccgatttct
    37561 cattggaaaa caatgctttt tcgggccgga ttgaggagga tattcactca aaagaagatg
    37621 atcatttgca atcacctctt gcaagtcacg attttcagca ggatagactt gctctatccc
    37681 tgttccaatg actgcgattg tccgtccacc atgagagatt gctgcttggt gaccgtatgt
    37741 atctatccct ttcgcgcagc cactgacgat cacatatcca gcttctatca ctggttttag
    37801 taaataataa actgtttttt tggctaaagc agaagtgtgt cttccaccaa caacgcttaa
    37861 catgttacaa gaaagtaaag atagatcacc attataaaac agtacaattg gcggataggt
    37921 caagtgaagc agctgagggg gatatttggg atcaaagcaa gtgataaacg actgtttcga
    37981 ttggattttg ccccagttag cgtgaatttc ctgccaactt ttgcgaaaca gctcccggtg
    38041 tttggttatt ttacctatct taatgatgtc ttcaaatccg atttctttat aaggatactg
    38101 catcatccag ttagcaagtg cccattttcc ttgactactg attccttttg tataagcaag
    38161 tttcaataaa ttttcttcta tttgatacat cataaaaccg cctttcgtct ctctttctaa
    38221 taagatacgt aaaagcggct gttttttatt gtaaatattt ttttacaggt gcaaaacttt
    38281 tacgatggat tggcgtaatt ccttgtcgtt caagtccttc taagtgttcc tttgttccat
    38341 aacctgcatt atgcgagaaa ccatatccag gataaagcac gtcatattct ttcatcaatt
    38401 ggtctcgata tactttagca atgatacttg cagcagcaat cgacacagat cgtacatccc
    38461 ctttgattat tttttcctgc tgaataggca aatccaaggt catcgcatcg atcaacaaat
    38521 agcttggtac aatggacaat ttttctactg cttggatcat cgctaattta cttgcttcat
    38581 aaatattcac atcatcgatt gtctgctgat caacgatccc gatcccgatt gccagggctt
    38641 tttcttgaac ttgttgataa agaagttctc gctttttctc tgataattgt ttggagtcat
    38701 tcagccctaa tatgatctca tttttcggca agataactgc agctgccacg acaggtccag
    38761 ctaatggtcc tcttcctact tcatcaatgc cggcaatcca ttggtgacct tgtgcgtacc
    38821 cttgcttttc aaagcaaaac atttcttcat atttttcctt aattaatgct tcctttttca
    38881 atcgtcgttc aaaagaagcc agtgcttgct gcacgccttt gcgtggatca tttcttaatg
    38941 attctagtgt aggatcatct attctctgta tgcttttcaa tcgttctttg atactttgga
    39001 tggattcagt catcactttt ttgctccatt tcttcgcaac gatccagtgt gtatcgtcca
    39061 agttttcctt gacgaatatc atgcacgact aattcgctgc cacgatcata gtcttcttta
    39121 tacccacgtt ttgctgtaat atccatcagt aattccggtg ccaatttttc ttcttgctcg
    39181 attgataaac cataacgatc gctgattctt cctgaataat atcgggcaaa aaagtctaat
    39241 ccgtaaatcg ctaaatcatc taaatggaca agctggtctt tgatcgcgcc tgtcagcgct
    39301 agttttttgc cgatttcttc atcttcaaat ttgggccata aaatccctgg tgtgtctaac
    39361 aattccaact cattacctaa acgaagccac tgctgacctt ttgttacgcc aggcttattt
    39421 cctgtctgtg caattttctt gcctactagt cgattcatca acgtagattt cccaacattt
    39481 ggaataccta agaccattgc ccgaatagcg cgaggcttga tcccccgagc agcatctctt
    39541 gcccgttttt ctttcaatgc ttctttagcc gcaggtacta gacctttaat tcctttactt
    39601 tcttgtgccg tgatagctaa tgcaagatgg ccttgtttac gaaaatattc ttgccacagc
    39661 tttgtctgtt gtggatctgc cagatccgct ttattcaata aaacaaggcg aggtttttgc
    39721 tgaatgatct gatctagcat tggattacga gaagataaag gtaatcgtgc gtctaccaat
    39781 tcaaatacga tgtccacata tttcattttt tcagaaactt ctcgtcgggc ttttgccata
    39841 tgtcccggaa accattggat ggtcattgtt ctctccattc ctttcatacc ggaatattcc
    39901 ccggctttac ttgttttttt ctttttcaat ataaattcac ttttttatta taaagaaatt
    39961 ccatctatta gttaagtgaa agtgaaccag tcctcattct atcacatctt cacttttttg
    40021 atgatgcaaa tttttatttt ggtttctata tatgaatcta aatttttgac agcgtttcct
    40081 aaataagaga tgatagacga acaaaacaga aaggagacga aacaaatgaa tagtatggtt
    40141 tttgttaatt ttcctgttgc tgatgtcaat cgttccgttg ctttttatga aaagctgggt
    40201 tttaaaaaaa atgaagaatt ttctactgaa gaagctggtt caatggtatg ggatgacact
    40261 ttttggataa tgcttctctc tcatgatttt tataaaaaat ttataaaaga taaagaaata
    40321 gctgataata aaaaaacaag tggtgctttg atttcgttta gtgttgaaaa tgcggatgaa
    40381 gtaaaaaaat ttgctcaaat tgcaaaagaa aatggtggta ctttccacga agtagatatg
    40441 ggaatgcctg aagaagaaat gttttcatta gaagtccaag atctagacgg aaatacatta
    40501 gaacccgtat ggatgaagat gtaagcagat aactaatctg acgtttttta taacaaaaaa
    40561 acaaaggcgg atgatacgat aagtattctc cgcctctatt ttttttattc gtacaatctc
    40621 ttttttacag tttataaaag cagtcttctc accctttttt taattgttag atgccagtgt
    40681 atgtaaatca ccaatgtgtc atgacacatt ggtgatttta tggtatcctt ttattattaa
    40741 ctgacaaagg agagaatatt ttgaacactt tgattgcaaa agacgttttt agattgcccg
    40801 aaatgaatgc aagattgttg tcgacctctg ctcttttatt agcccttgat gtcatcttat
    40861 acaagatagc gattggtcct gcttattttc aaatcacatt cggattttta agtatggcgc
    40921 ttatgggtta tctatatgga ccgatctggg ctggtttgct tgaaatgttg tcgaacactt
    40981 taaatttcac gatatttggt tcaggaacgt ttcaagctgc ttttcttatc acggctttcc
    41041 ttggtggatt tattaatggc ttatttttat atcataaaaa gataaacctg ccttccgtca
    41101 tcgctgccca attattgatt atgatcccag tcagcctttt tatcaattct tggctgatgt
    41161 ctgttttgtt tggtgcagat ttcaaagtaa tttttctagg tagattcatc aggaatatga
    41221 tccaaatccc tattcaaata atggttgttt attcatttat caaaacttta gaggctagaa
    41281 aagtgtttaa acgagctgtt tgattttacg tactaaggta gtaaactgat tttggattaa
    41341 acaataaagc gtgcagtctc aacagactgc acgcttt
//
LOCUS       DAPBCS010000024        40919 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPBCS010000024 DAPBCS010000000
VERSION     DAPBCS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 40919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.024"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..421
                     /locus_tag="RD521_001444"
     CDS             191..421
                     /locus_tag="RD521_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDT7771188.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="RD521_001445"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="RD521_001445"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDT7771189.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="RD521_001446"
     CDS             1832..2008
                     /locus_tag="RD521_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDT7771190.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="RD521_001447"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="RD521_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDT7771191.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="RD521_001448"
     CDS             2624..3091
                     /locus_tag="RD521_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771192.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="RD521_001449"
     CDS             3176..4060
                     /locus_tag="RD521_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7771193.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4174..4410
                     /locus_tag="RD521_001450"
     CDS             4174..4410
                     /locus_tag="RD521_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDT7771194.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4498..4578
                     /locus_tag="RD521_001451"
     tRNA            4498..4578
                     /locus_tag="RD521_001451"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4532..4534,aa:Tyr,seq:gta)
     gene            4585..4656
                     /locus_tag="RD521_001452"
     tRNA            4585..4656
                     /locus_tag="RD521_001452"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4617..4619,aa:Gln,seq:ttg)
     gene            4781..4951
                     /locus_tag="RD521_001453"
     CDS             4781..4951
                     /locus_tag="RD521_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771195.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5171..5878
                     /locus_tag="RD521_001454"
     CDS             5171..5878
                     /locus_tag="RD521_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7771196.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5937..6581)
                     /locus_tag="RD521_001455"
     CDS             complement(5937..6581)
                     /locus_tag="RD521_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDT7771197.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6707..6994
                     /locus_tag="RD521_001456"
     CDS             6707..6994
                     /locus_tag="RD521_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771198.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7102..7422
                     /locus_tag="RD521_001457"
     CDS             7102..7422
                     /locus_tag="RD521_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7771199.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7503..8816
                     /locus_tag="RD521_001458"
     CDS             7503..8816
                     /locus_tag="RD521_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDT7771200.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8813..9532
                     /locus_tag="RD521_001459"
     CDS             8813..9532
                     /locus_tag="RD521_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDT7771201.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9660..10211
                     /gene="raiA"
                     /locus_tag="RD521_001460"
     CDS             9660..10211
                     /gene="raiA"
                     /locus_tag="RD521_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDT7771202.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10433..12967
                     /gene="secA"
                     /locus_tag="RD521_001461"
     CDS             10433..12967
                     /gene="secA"
                     /locus_tag="RD521_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDT7771203.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13093..14194
                     /gene="prfB"
                     /locus_tag="RD521_001462"
     CDS             join(13093..13164,13166..14194)
                     /gene="prfB"
                     /locus_tag="RD521_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDT7771204.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14299..14985
                     /gene="ftsE"
                     /locus_tag="RD521_001463"
     CDS             14299..14985
                     /gene="ftsE"
                     /locus_tag="RD521_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDT7771205.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14969..15862
                     /locus_tag="RD521_001464"
     CDS             14969..15862
                     /locus_tag="RD521_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771206.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16014..17462
                     /gene="cls"
                     /locus_tag="RD521_001465"
     CDS             16014..17462
                     /gene="cls"
                     /locus_tag="RD521_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDT7771207.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17799..18656
                     /locus_tag="RD521_001466"
     CDS             17799..18656
                     /locus_tag="RD521_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDT7771208.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18771..19691
                     /gene="pstC"
                     /locus_tag="RD521_001467"
     CDS             18771..19691
                     /gene="pstC"
                     /locus_tag="RD521_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDT7771209.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19691..20575
                     /gene="pstA"
                     /locus_tag="RD521_001468"
     CDS             19691..20575
                     /gene="pstA"
                     /locus_tag="RD521_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDT7771210.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20590..21396
                     /gene="pstB"
                     /locus_tag="RD521_001469"
     CDS             20590..21396
                     /gene="pstB"
                     /locus_tag="RD521_001469"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDT7771211.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21412..22170
                     /locus_tag="RD521_001470"
     CDS             21412..22170
                     /locus_tag="RD521_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDT7771212.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22183..22860
                     /gene="phoU"
                     /locus_tag="RD521_001471"
     CDS             22183..22860
                     /gene="phoU"
                     /locus_tag="RD521_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDT7771213.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23098..24129
                     /locus_tag="RD521_001472"
     CDS             23098..24129
                     /locus_tag="RD521_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDT7771214.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24126..24893
                     /locus_tag="RD521_001473"
     CDS             24126..24893
                     /locus_tag="RD521_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDT7771215.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24993..26561
                     /gene="liaX"
                     /locus_tag="RD521_001474"
     CDS             24993..26561
                     /gene="liaX"
                     /locus_tag="RD521_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDT7771216.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26858..27178
                     /locus_tag="RD521_001475"
     CDS             26858..27178
                     /locus_tag="RD521_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDT7771217.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27178..27528
                     /locus_tag="RD521_001476"
     CDS             27178..27528
                     /locus_tag="RD521_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDT7771218.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27695..28633
                     /locus_tag="RD521_001477"
     CDS             27695..28633
                     /locus_tag="RD521_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDT7771219.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28648..29478
                     /locus_tag="RD521_001478"
     CDS             28648..29478
                     /locus_tag="RD521_001478"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDT7771220.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29522..30547
                     /locus_tag="RD521_001479"
     CDS             29522..30547
                     /locus_tag="RD521_001479"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDT7771221.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30562..31500
                     /gene="galU"
                     /locus_tag="RD521_001480"
     CDS             30562..31500
                     /gene="galU"
                     /locus_tag="RD521_001480"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDT7771222.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31625..32065
                     /locus_tag="RD521_001481"
     CDS             31625..32065
                     /locus_tag="RD521_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDT7771223.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32068..32670
                     /locus_tag="RD521_001482"
     CDS             32068..32670
                     /locus_tag="RD521_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7771224.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32729..33835)
                     /locus_tag="RD521_001483"
     CDS             complement(32729..33835)
                     /locus_tag="RD521_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDT7771225.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34045..35046
                     /gene="ccpA"
                     /locus_tag="RD521_001484"
     CDS             34045..35046
                     /gene="ccpA"
                     /locus_tag="RD521_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDT7771226.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35383..37728)
                     /locus_tag="RD521_001485"
     CDS             complement(35383..37728)
                     /locus_tag="RD521_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDT7771227.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38007..38909
                     /locus_tag="RD521_001486"
     CDS             38007..38909
                     /locus_tag="RD521_001486"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDT7771228.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39122..40897
                     /locus_tag="RD521_001487"
     CDS             39122..40897
                     /locus_tag="RD521_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDT7771229.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
BASE COUNT        13326 a         7107 c         8720 g        11766 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa acctttcgga
     4501 ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg ttcagtggtt
     4561 cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa gggactttga
     4621 ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt aagctctcgt
     4681 tagttcgaga gctctttttt ttgctttgct accttgttcc ttgaagattc tgctgattta
     4741 agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga aagaaaaagt
     4801 agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca atattgaagt
     4861 gaatattccg aaaaaaggcg gaaaagcggt ccgggtaaaa tcaaaacgcc caacgaatca
     4921 tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata agtcggcatt
     4981 ttcatcgatt ttagggtctt ttgttatttt ctaggttgga catatctaaa aaattgtttc
     5041 tgacaatagg agtaggaaga acgatgtgtg tctaaatcct ctgttgatgg atagaaacct
     5101 ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa cgaaaggacg
     5161 ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc aattaaaaaa
     5221 tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa gagaattagc
     5281 agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact tggcagatga
     5341 aggaatctta gaaagaaaga tcggttcggg tacatatgtt gcacgagaaa aagtacaaga
     5401 aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc gagtaccgtc
     5461 tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg aaaaactaaa
     5521 cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag atgatgtccc
     5581 tatttgtttt gaagttgcca gtgtgccaca aaagctgatc gatggctaca gcaaagcaga
     5641 gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga tcggtccagc
     5701 gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt tagaggtaaa
     5761 acgaggagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg gtacgccgtt
     5821 tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg aaaaataaaa
     5881 aacaggtgat ccctgattcc gtattagaaa taaaagagaa tcagaatcac ctgtttttat
     5941 atatgatcag ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt
     6001 ccgttcagca gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca
     6061 cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca
     6121 agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc
     6181 aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg agtgtatgct
     6241 cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct
     6301 acattgatca gttgattttt tttgaggact tccaacatcg gtatgcccgc cgttccactt
     6361 ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg
     6421 cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttcg
     6481 tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat ctcgatctca
     6541 ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc tcttttttca
     6601 ttttctttat tcagtataaa taaagttcta ggttgtttca acactgtaac ggttgcgcaa
     6661 ttgctgtagt atgcgataat aaagacaaag gcaggtggat ctggggatgc aggaacgaat
     6721 gaaaaagtac gatgcaatta cgcattattt gaagaacaat ggcggctctc aagtaacgtt
     6781 gacctttacc caatttgacg aactgctgtt tcccagtaac ggacttccca aaacagcaag
     6841 ggaaagtaca gattggtggg caaatgatta taaacaccct gaaaaaggtg cctatggctg
     6901 gataaatgca ggctatgaag tagttgtcat caatttggac aaagaatatg tcgtgttcaa
     6961 taaattagtg aaatctagtt ggctatttga ttaaataaac cgcgtttaga aaacgttgac
     7021 aaatttcttt ctttttatat aatagagtaa cagacgcgaa gaaaacgttt gcgtagtttg
     7081 ttttaaaaag gagagaaaag tatggctaaa aaaacaatca tgcttgtatg ttcggctggt
     7141 atgagcacaa gtttactagt aacaaaaatg caaaaagctg ctgaagcaaa aggaatcgac
     7201 tctgatattt tcgctgtgtc cgcttctgac gcagataaca atcttgcaaa taaagacgtg
     7261 gatgtgttgt tattaggccc acaagttcgc tttatgaaag ctgattttga gaaacgtctg
     7321 gaaccaaaag gcattccttt agatgtcatc aatatggctg attatggtat gatgaatggc
     7381 gaaaaagtgt tagatcaagc aatcacgctc ataggtgaat aaatagaaca gtataactat
     7441 cgaacaagcg atccaaaaaa tagatcgctt gttttttcgt atattttttt agggcgaata
     7501 atatgaatca tttttggggt agaaggcttc tgacgagaga tattaaagca atagactgtg
     7561 aggaggtaaa tttaccaaat tatgagaaga tcgcagcagt ggaacgtttg agcaactgga
     7621 ttctttgtca tcgctgtgga atgagaacgt ccgtcattga tggacagctt gcagattacg
     7681 gttatttttg tacacattgt ctatatttag ggcgatgcga cactcaacaa gatttatatt
     7741 tgtttgatca gcctgaagca aaacctcgag aagtcgtctt tttttggaca ggacagctta
     7801 ctgaaaaaca aacggaaatc tcaaagagaa tactttgcca ttcagaaaaa agacatcatc
     7861 ttatttgggc tgtaacaggt gcagggaaaa cagagatgct ttatcctatt ttagtggaaa
     7921 ctttaaaagc tggaggaaga gtagcgattt gtacaccgcg agtcgatgtt tgtaatgaat
     7981 tgtttttacg ctatcgttat gtttttccag aagaaaaaat cacgttgctc catgggaata
     8041 cagcaacggc ttataccttt acttcttttg ttatctgtac gattcatcag ctataccgtt
     8101 tttatcggtc ctttgattta atagtgatcg atgagatcga tgcgtttcct tataacgggg
     8161 atgcaggatt agctcatgct gtgcaaacag cgattaaacc agacggacga ttcatctact
     8221 taagtgccac acctgataaa aagttactaa aggagatcaa gcagacgttt gacatccaca
     8281 aacttgcttt acgctttcat cggcgtttgc ttcctgaacc cgtcttggtt ttcatgaata
     8341 attggcgaca aaaatgcaag aacaagaaga agataaaatc tttggtgcgg atcattcatc
     8401 aattaattaa agaaaaccat atccttttat tttgtccaag tatcacgatg atgcatcgtt
     8461 tgaaagccac acttcaagag gctcttccga gatatgagat cacagatgtt tcttcaagag
     8521 acaatcagcg ggcggaaaaa gtccagaaaa tgcgagaagc aaaatatgac atactgttaa
     8581 ccacgatgat ccttgaacgt ggagtcacat ttgaaagaat atccgttatc gttttaggag
     8641 cggatcatcc ggtttttact aaatcttctt tggtacaaat cgcaggaaga gccgatcgta
     8701 aagggcctta tacgaacagt caagtatatt ttttctatga agaaaaaact gcagctattc
     8761 gaaaagcttg tcaggaaatc aaagaaatga atgaagaagg gaagaagata ctgtgaggtg
     8821 cagttgctgc agtaaagata tcgtccaaaa tttaacattc agcgagatta ttggtgtttc
     8881 gaaatacaga ttacctcaga cttgttcagt ttgtcgcgca caatttcatt tattggaaac
     8941 aagaggctgt tctggctgtc aaaaaccaat ccatttcaat ctagagagta cgaaaaagaa
     9001 agtaaattct atccccgttt actgcagtga ctgtcaagaa tggaagaaga aatatccgac
     9061 ctatgatttt cagcaccgtg ccttttttgt gtacgatgaa aatatgaagg cctggctcaa
     9121 tcaatacaag tttatgggag atatccgact agcaggcacc tttgcatata aatggacgca
     9181 gataaaaaaa gaatacccga catacatcta ttgtccaata cctttatctg aaaaaaggcg
     9241 aacagaaaga ggattcaatc aagtaagcga gatgttgaaa gcagcaagaa ttccttttca
     9301 gatgctactc aaaagagaga agcatctgct ggctcaagca aaaaacacgc gaaaggaaag
     9361 attggagatg cctcaacctt ttgaacttgc agtcaccttt tctaagatac aaaataaaaa
     9421 aatattgatt attgatgatg tctatacaac aggacagact cttttccacg cagcagattg
     9481 tttgcagatc gcacagccga aaatcatccg aactatttct ttggcgagat gaattagatg
     9541 tggaaatttg atagcgattt aatttgcaaa taacctagag ttcgatataa tagaattaag
     9601 aagagggaaa gagtggtcct tctccttctt gattgtggta gacgaaaggg gtaattttta
     9661 tgtttagata taatgtacgc ggcgaaaata tcgaagtaac tgaagcgatt cgcgactacg
     9721 ttgagaaaaa agtaggcaaa ctagaacgct atttcagtga cgcacccgat gcaacggcgc
     9781 atgtgaactt aaaagtttat acagaaaaaa cagcaaaagt ggaagtaacg atcccattac
     9841 cttatcttgt attacgtgcc gaagaaactt ctccagattt atacgcaagt atcgatttgg
     9901 ttgttgacaa attggaacgc caaatccgca aattcaaaac aaaaattaat cgtaaatccc
     9961 gtgaaacggg catgaatact gcaaatgctg ctgctttatt caatgaagag gacgaaaacg
    10021 aaaatgattc tgaattagat atcgtacgta cgaaacgcct ttcattgaaa ccaatggata
    10081 gtgaagaagc agtcttgcaa atgaacatgc tgggacataa cttctttatt tttgaagatg
    10141 cagaaacaaa tggtacaagt atcgtttacc gccgtaaaga tggaaaatac ggactgattg
    10201 aaacagactg atcagcctta cttaaataac aacgaaaact tgcagcagct actgacagct
    10261 gccgcaagtt tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt
    10321 cttactttaa tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta
    10381 aagtctacat agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa
    10441 tttgattcgt tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc
    10501 aaaaaaagtt gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc
    10561 aaaaactgct gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc
    10621 ggaagctttt gcagtcgtcc gagaagcagc aaaacgtgta ttaggtttat atccatacca
    10681 cgtacaattg atgggtggaa tcgtgcttca cgatggaaat atccctgaaa tgcgtactgg
    10741 tgaaggtaaa accttgaccg caacgatgcc agtatatcta aatgctctga ctggcgaagg
    10801 ggtacacgta gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga
    10861 actttataat ttcttaggat taacagtggg gttgaatatc aactcaaaat cttcggatga
    10921 aaaacgcgag gcttataatt gtgatatcac ttatagtacg aataatgaat taggatttga
    10981 ctacttgaga gacaatatgg tcgtttaccg ccatcaaatg gtacaacgtc cgctgaatta
    11041 tgccattgtc gatgaagttg actcaatctt gattgatgaa gcgagaacgc cgctgatcat
    11101 ttcagggcaa gcagaaaaat ccacagcttt atatacaaga gcagacaact tcgtgaaacg
    11161 tttgaaagaa gaagaagatt ataaaatcga tgtacaatca aaaacaatca gtttaaccga
    11221 agcagggatc gaaaaagcag aagaaacatt cggtttggat aacttatacg acattgaaaa
    11281 tactgcattg acccatcact tggatcaagc attgagagcc aactatatca tgcttttaga
    11341 tatcgattat gttgtccaag acggaaaagt actgattgtt gaccagttca ctggtcggat
    11401 catggatgga cgccgttatt cagatggact tcaccaagca atcgaagcta aagaaggcgt
    11461 agagatcgaa gacgaaacga aaaccatggc gacgatcact ttccagaact atttccgtat
    11521 gtacaagaaa cttgcgggaa tgactggtac agctaaaaca gaagaagaag aattcagaga
    11581 aatttataac atccaagtat accagattcc gacaaatcgt ccggttatcc gtgatgatcg
    11641 tccagatttg ctttacccaa cattacaaag caagttccac gctgtcgtgc aagatatcaa
    11701 agatcgctac cgcaaaggtc aacctgtgtt agtcggaact gttgcggttg aaacatctga
    11761 gttattatca gatatgctga atcaagaacg tattcctcat gaagtgctga atgcgaaaaa
    11821 tcactttaaa gaagcagaga tcatcatgaa tgctggtcaa aaaggtgctg tgacgattgc
    11881 taccaacatg gctggtcggg gaacagatat caagttagga cttggggtaa gagaacttgg
    11941 tggtctagca gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg
    12001 aggacgtgca ggacgtcaag gagaccctgg tatgtcacag ttctacttgt cattggaaga
    12061 tgatttgatg aaacgattcg gttcagaacg aatcaaatct ttcttagatc ggatgaagat
    12121 cggtgaggaa gatgctgtta tccaaagcaa gatgctgacg aaacaagtag aatctgcaca
    12181 aaaacgtgta gaaggaaata actatgacac acgtaaaaac gtcttacaat acgatgatgt
    12241 gatgcgtgaa caacgtgaag taatctataa acagcgacaa gaagtaatca tggaagaaaa
    12301 aagcttatcc aagccactaa tgaacatggt caaacggaca atcagtcgtg tagtagatgc
    12361 gcatacacaa ttagagaaag aaaattggaa tttggaaagt atcatcgatt ttgccggcag
    12421 tactcttgtc catgaagata gtattactct tgcagatatc gaaggaaaaa cagctcagga
    12481 gatcaaagag tatctaaacg aacgagcaca agaagtattt aataccaaag cggctcaact
    12541 aaatggtcca gagcaactgt tggaatttga aaaagtcgtg atcttgcgtg ttgtggactc
    12601 taaatggaca gaccatattg atgcaatgga tcaattacgt cagtcaatcg gcttacgtgc
    12661 ttatggacag aacaatccat tagtcgaata tcaaacagaa ggctataaga tgtttgaaga
    12721 tatggtaggc gccatcgagt atgaagtcac tcgaatcttt atgaaagctg agatccgtca
    12781 aaacgtacag cgtgaacagg tttctcaagg aacagcttct cactcagaag acggagatgc
    12841 caaagaaaat agtaatacta gcgcgaaaaa acagccaatc catgtgaaca aggtcggaag
    12901 aaatgatcct tgtccttgtg gaagcgggaa aaaatataag aactgtcatg gaaaaaatgc
    12961 aaactaattt ctgacagcag gattggtgag acaaattctt tttttaagaa ttcgtctcac
    13021 cttctttctg ttatttgctg attctgttag actagatata atgaaaaaag tatagtgaaa
    13081 gaaggaaaaa acatggaaat atccgaaatt cgaaatctcc tgacagaaat gcaggaaaag
    13141 gtcacaagct tcaggaggtc tctttgactt agaccagctg gaagaagaca ttgcggaagc
    13201 agaagcaaaa atggctgaac ctggtttttg ggatgatagc gaagctgctc aaaaagtgat
    13261 cagtgaaaac aatgcaaata aagaaactta tgatcaattc cattctcttg ctgaagaact
    13321 ggaagaaatc gaattgatgg tggagatgtt gcaggaagaa cctgatgcag ccatgcaaga
    13381 agaagcagaa acacgtatca atgcgctaga tgaaaaaatg aagacatatg aattatctat
    13441 gttgttagat ggtccttatg atcgaaacaa cgccatcgtt gaattgcatc caggggctgg
    13501 cggaacggag tcccaagatt ggggcagtat gcttttacgt atgtatacac gctgggcaga
    13561 gcagcatggg tatcaagtag agacactaga ttatcaagca ggtgatgaag caggaatcaa
    13621 gagtgtgacc ctactgatca aaggacacaa cgcatttggt tatctgaaat cagaaaaagg
    13681 tgttcatcgc cttgtacgta tctcgccttt tgactcagcg aaacgacgtc atacttcttt
    13741 ttgttcggta gatgtgatgc cagagcttga tgaaacgatc gatatagaaa tcaatccaga
    13801 tgatttgaaa atcgatacgt accgagcaag tggtgcaggt ggtcagcaca tcaataaaac
    13861 agaatctgct gtacggatca ctcacttgcc tacaggaaca gtagtagcta gtcaggcaca
    13921 acgttcacaa ttgaaaaacc gtgagcaagc gatggggatg ttaaaagcta agctttatca
    13981 attagaagtg gaaaagaaag aacaagaagc agctgcactt cgaggagaac aaaaagaaat
    14041 cggctggggt tcacagatcc gttcttatgt tttccatcca tattctatgg taaaagacca
    14101 tcggacaaac gtagagacag gaaatgtcca agcagttatg gacggtgacc ttgatttatt
    14161 tattgacggc tatttaaaaa tgcatctaca ataagaaccc attgttaaat tgaaataaaa
    14221 ttgtaagatt ttgcaagcat gttgaaacat tgccatgcta taatagctaa agactgaaaa
    14281 agatatggag agaacgccat gattgaaatg aaggatgtaa tgaagaagta ctcaaatggt
    14341 acgaccgcta tccgcaatct ctcaattgtc atcgaacaag gagagtttgt ctatgtggtt
    14401 ggtccgtctg gagccggtaa atctacattc atcaaattga tgtatcgaga agaaaaagct
    14461 tcaaaaggaa ctttgaacgt cgcaggtcat gatctaatag caatcaaaaa ccgcgaagtt
    14521 ccttatctac gcagagaaat cggcgtggtg ttccaagact ataaattatt accaagaaaa
    14581 acagtatatg aaaatgttgc gtacgctatg caagttatcg gcagaaaacc tcgggatatc
    14641 aaaaaacgag taatggaagt gctggacctt gttggtttga aacacaaagt tcgagtcttt
    14701 cctagcgaat tatccggtgg ggaacagcaa cgtgtctcta ttgctcgggc aatagtgaat
    14761 acgcctaaag tacttatcgc ggatgaaccg acaggaaatc tggatccaga aaactcatgg
    14821 gaaatcatga aattattaga tcggatcaat gcccaaggaa ctactgtcgt gatggctaca
    14881 cataacagta cgatcgtaaa tacgattcgg caccgagtaa ttgctatcga aaacggccgc
    14941 attattcgag atcaagcgga aggggaatat ggatacgatg attagaacct ttttccgcca
    15001 tttattagaa agtatcaaga gtttgaaacg taacggctgg atgacagttg cttcagtcag
    15061 tgcggtaacg atcacattga ctttagtcgg tgtattcatg gcagttatca tgaacgcaac
    15121 taaattagct caagatatag aaggaaacgt tgatgtctct gtattcgtag atatcggtac
    15181 aaaagacgaa gagatgaaaa agctagaaaa agaattgaat gacttagatc atgttaaaaa
    15241 agttgaattt tcaagcaaag aacaggaatt gaaaaaaatc caaaacgaaa tgggtgattc
    15301 ctggaaattg tttgaaggtg acaataaccc gttatatgat gtgtatgtag taagtgctac
    15361 tgatccttct tatacaaaac aagtagcaga agaagcagca gatttgaaga atgtttctcg
    15421 ttctgattac ggtggagctt cctcagatcg gattttccaa atcgctggtg cagttcggac
    15481 atggggatta gttgcagcgg cattgctgct gttcgtagcg atgtttctca tttcgaacac
    15541 gattcggatc acgattttgt ctagacagcg tgaaattcaa atcatgcgtt tagtcggtgc
    15601 aaaaaatgga tatattcgct ggccgttttt ccttgaaggt ggctggatcg gtctgatcgg
    15661 tgcgattctt ccgattttga tcatcacatt tggttatgca tggtttttcc aattgatcaa
    15721 tccatcatta ttacgttccc attattcatt gattcatcca caaaatatcg tttggaaaat
    15781 caatctttta atggtcggca ttggtgtgat cattggttca cttggctcga tcatttctat
    15841 gagacgtttc ttgaaattct aataccacaa tataaataaa atgagaaagt tggacagttt
    15901 cgttccagct ttcttttttt attggctagg agaatagaag gaagcggctt ttttcccttt
    15961 actttaaaaa acaacttggg tattcttaag ttggtagaag aagaggtgaa gccgtgaaaa
    16021 ttatcgttga caattttttt acgatccttt tgattatgaa tattttgctt tcctttatca
    16081 tcgtttttcg agaaagaaag gaaactgctc aaacatgggc atgggtgctc gttttgatgt
    16141 tcattccagt tgtaggattt attttatata tttttttagg aagagggatt tccaaagaga
    16201 aaatctttga tttgaagatc caagggaaaa tcggtaagaa cctggaaatt gaagaagaca
    16261 aacaggcatt gatgcgaggg ctttatcctc atccgcctac tggacaagta gatgtgaaac
    16321 aattgatcta tatgctcacg gtatttgaaa gtacgctcta tacgactaaa aatgaaatca
    16381 ctttatttac agatggtcgt gaaaaattcg atgcgctgat tgaagatatc aagcaggcag
    16441 aagaccatat ccacttccag tattatattt atcgcagtga tgcattaggt gaagaagttc
    16501 gagatgcatt gacagaggcg gcaagacgag gggtaaaagt cagagtcctt cttgatgcat
    16561 ggggttccac acaagtttct tccagctttt ttcagaattt gaaaaaagca ggcggagaag
    16621 tcgcattttt ctttcctcta tttgttccgt atattaatcc tcggatcaat tatcgcaatc
    16681 atcgtaaaat cgtagttatt gatgggaaaa tcggatatac aggtgggttc aatgttggaa
    16741 atgaatatct aggattggta aagaaatttg gttattggcg agacaatcat ttgcgtattt
    16801 acggagaagc ggtgtatacc ttgcaaaacc gttttttgat ggactggaat tctcagcata
    16861 caaaagaagt aggctattct cccaaatttt ttccgtccat tcactctact ggtgatatag
    16921 ctgttcaaat cgtgacaagc ggaccagata ccgaacatga acaaatcaaa atgacttatt
    16981 tgaaaatgat ttcgttagcc aaacgggaga tattgatcca aacgccttat tacataccgg
    17041 atggatcgat tcatgaagca ctgaagttgg ctcttctttc aggcgtaaaa gttcatatcc
    17101 aaatacctaa caaaccagat catcttctcg tttactgggc aacgtattct ttcgctgcag
    17161 agctaatcga gtatggagca aggatcgaaa catatgagaa tggatttatt catgcaaaga
    17221 cgatgattat cgatggtggc attgtttcag ttggctcagc aaatatcgat gttcgttcat
    17281 ttcgcttgga ctttgaggtc aatacactgg tttatgatga acgaatggct tcacgtgtaa
    17341 gaaaagcttt ttttgaagac tcgaaaattt ctacgcatct cacaaaagaa atgtatgaaa
    17401 accgaggaat aatcatcaag atgaaagaag gattagcaag attgatttcg ccgctccttt
    17461 aaaacataaa aaggacatga gaaccatcaa cagatggaga tctcgtgtcc ttttctaacg
    17521 gggccatttc tgagagtacg tcagaaatgg agaaagcgtc tcgcatgagc gaaattaact
    17581 ttggggtatc tcttgttttt tggggaaagc aagaaataag ctaatatatg ctattaaaag
    17641 agacacattt caaagccagt atagcataga aacttttaat gagatagttt tttttacaat
    17701 tatgagaatt tattttgtta tttaatgatt ttgattcgtt ttcatcaagt aatgaaaatg
    17761 ataaaataaa catactttag gattaaagga gatttctaat gaaaaaactt aaattattct
    17821 taccaataat aggattagct attcttttat caggctgttc aaagtggatt gatcgaggag
    17881 aatccattac ggctgttggc tcttctgctt tacagccttt agtagaaaca gttgctgaaa
    17941 cttaccagac agaaaatcct ggaaaattca tcaatgtaca agggggcgga agcggaactg
    18001 gtttgtctca agtacagtca ggagcagtcg acatcggaaa ctctgatttg tttgcggaag
    18061 aaaaatcggg gatcgatgcc tcagcattag tcgatcatcg agtagcagtt gttgggatca
    18121 ctcctattgt gaacaaggat attggcgtaa aagacatcag tatggaaaac ctgaaaaaac
    18181 tatttacagg cgagatcaaa aactggagtg aagtcggtgg aaaagatcag ccagtagtta
    18241 tattgaaccg cgcttcagga agcggaaccc gtagcacgtt tgaaaaatgg gtattggacg
    18301 gagaaacagc tgttcaggca caagaacaag attcaagcgg aatggtgcgc cagatcgttg
    18361 ccgacacacc cggtgcaatc agctatgttg ctttttctta tgtttccgat gatgtagata
    18421 cgttgaatgt taacggtgta gaaccgacag atgaaaatgt gacaacgaac aaatggacga
    18481 tttgggcata tgagcatatg tacacaagag atcatccaaa aaaactgaca aaagaatttt
    18541 tagactatat cttatctgat gaagtacaag ataacatcgt tggagaacta ggttatatcc
    18601 ctgtttctca tatgaaagtc gaacgtgatt ggcaaggaaa tatcgttaaa gagtaatctt
    18661 tacacaaaat ttacaaactt agctaaactt ttacatgtta ttaaaaccaa attgatgttc
    18721 aatttgtaca ctgagtgagt atgttaaacc tgattaaggg ggagtcatct gtggaagatg
    18781 tgaaaaaagt attattaaca aaatcaaaac gttcaaaaat ggaacagcgt ggaaaattca
    18841 tcagtttttt atgtatcgcg ctgatcgtgt tggttgtatt atctattttt tattttgtag
    18901 ctagtaaagg attggccacc ttttttactg ataagatcaa tgtatttgat tttctgtttg
    18961 gtacagattg gaatccaagc caaatcggag caaatgggaa accaatggta ggggcattgc
    19021 ctatgatcac aggttcgttt attgtgacct ttttatctgc tatcgttgcc acgccatttg
    19081 ccattggtgc agcagtattc atgacggaga tctctcctaa aaacggaacg aagatcttac
    19141 agccggtcat cgagctgctt gtaggtattc cttctgtcgt ttatggtttt atcggtttat
    19201 cagttatcgt gccttttgtc agaagtattt ttggcgggac tgggtttggt atcttagctg
    19261 gtacatttgt gttgttcgtt atgatcttgc cgactatcac aacgatgact gttgatgcat
    19321 taaaggcagt gccacggcac tatcgggaag cttcattagc gcttggtgcc acaagatggc
    19381 aaacaatcta caaagtcgtg ctacgagcag ccgtgcccgg gattttaaca gcagtcgttt
    19441 ttggtatggc tcgtgcgttt ggtgaagcct tagctatcca aatggtcatc ggtaatgcag
    19501 cattgatgcc aacaggtctt actactcctg cttctacatt gacgtccatc ttaacaatgg
    19561 gaatcggaaa tacaatcatg ggaacagttg aaaataatgt gttatggtca ttagcgctga
    19621 tcttgctatt gatgtcatta ttctttaata tcgtgatccg tatgattggg aagaaaggag
    19681 agctgaaata atgaacgcaa aacgctcaga taaaattgct acaggtgttt tatatatagt
    19741 ttcggggatc atcgtattga ttttagcagc tttactactt tatatcttag ttcggggaat
    19801 accgcacatt tcatggtcct tcttgaccga accttccaaa gcctaccaag aaggcggtgg
    19861 aatcgggatc cagctattta actcgattta tttgcttttg atcacgatga tcatcagttt
    19921 tcctatttct ttaggtgcgg gtatctatct ttcagaatat gctggaaaaa actggctaac
    19981 agatgtgatt cgcacgtcga ttgaaatctt gagctcgttg ccttctgtcg ttgtcggttt
    20041 attcggtttc ttggttttcg ttattcaatt ccagtatggt ttctcgatta tttctggtgc
    20101 attggctttg actgtattca acttgcctct tttgactaga acagttgagg agtctttaca
    20161 agcggtccat tacacacaac gagaagcagg attggcctta ggtttgtcac gctgggaaac
    20221 agtgataaaa gtggtggttc cagaagcgac accagggatt ttgactggtg tgatcctaag
    20281 ttctggaaga atttttggtg aagcagctgc attgatctat acggctggac aaagtgcacc
    20341 tgcgttagat tttacgaact ggaatccatt aagtgtttca agcccgatca gcattttccg
    20401 tcaagctgaa acattagctg tccatatttg gaagatcaat acagaaggca cgatgcctga
    20461 tggtgtttct gtttcggctg gcgcttctgc cgtgttgatt attgctgttt tactgttcaa
    20521 cttcggcgct cgtgcgattg gcaaacgatt ataccgaaaa atgacttcag cctaaggaga
    20581 agaagcaaaa tgaaaaaata taactggaat gaaacaaatg tcatcaccat gccaaaagag
    20641 aaagaagtag cactatatac ggatgatcta catgtctggt atggcgacaa tgaagcaatc
    20701 aaaggagtgg atctggaatt tgaaaaaaat aagatcactg cactgattgg tccttccggc
    20761 tgcggaaaat ctacgtatct tcgttcgttg aatcgaatga atgatgggat cgctaatacg
    20821 aaaatcactg ggaaaatcat gtatcaaggc gtagatatta atgcgcctca agtggatgtt
    20881 tatgagatgc gcaaacggat tggcatggtt tttcaacgtc ctaatccgtt cagcaagtcg
    20941 atttatgaca atattacatt tgctttgaaa cagcacggcg aaaaaaacaa aaagaaatta
    21001 gatgaaatcg ttgaaacaag cttgaaacaa gctgccttgt gggaccaagt aaaagacagc
    21061 ttggacaaaa gtgccttggc tttatctggc gggcaacagc aacgtttatg tattgctcgt
    21121 gcgatcgcga tgaaacctga tatcttgctg ctagacgagc cggcaagtgc tttggaccct
    21181 atttcgacag gaacggttga agaaacactt gtaaacttga aagaagacta tacgatcatc
    21241 atcgtgacgc ataacatgca acaagcagct cgtatcagcg actatactgc ctttttctat
    21301 ttaggaaaag taatggaata tgacaaaact aacaagatat ttaccagacc aaaaattcag
    21361 gcaaccgaag attatgtgtc cggtcatttt ggttaagacg gaggcctcaa catgacaaaa
    21421 gagattattt catctaaaga tcttcatctg tattatggaa agaaagaagc actaaaaggg
    21481 atcgatctaa gcttcaatca aggggaaatc actgcgatga tcggtccttc tggttgcgga
    21541 aaatccactt atctgcgttc tttgaaccgg atgaatgacc tgattccgaa tgtaacgatc
    21601 accggaagtg ttttatataa agacaaagac atctatggtc caaaaacaga tgttgttgaa
    21661 ctaagaaaag aaatcggcat ggtattccag cagccaaacc catttccttt ttctatctat
    21721 gaaaatgtca tctatggatt gaaattaaaa ggggaaaaag ataaaaaagt ccttgatcaa
    21781 gtagttgaag aaagcctgaa ggcagcgtct gtttgggatg atgtgaaaga caaattgcat
    21841 aaaagtgctt tatctttatc cggtgggcag cagcagcgtg tatgtatcgc acgtgttttg
    21901 gcagttaatc cggaaatcat cttattggat gaaccaacaa gtgcgcttga tcctgtttct
    21961 actgggaaaa tcgaaagtat gctgttagaa ttaagagagc ggtatacgat gatcatcgtg
    22021 acccataata tgtctcaagc ttcacgtatt tcagataaga cggcattctt tttagatggt
    22081 cacttgatcg aatacaatga tacgaagaag atattcatga atccagaaaa acaagagaca
    22141 gaagattata tttcaggtcg atttggataa ggaggaaacg gaatgctacg tacacaattt
    22201 gaagaagaat tgctgaatct ccataatcag ttctacgaaa tggggatgat ggtgagcagc
    22261 gccgtgcaca agtcagttcg tgcatacatc aaacacgata aaatcttagc ccaagaagtt
    22321 atcgaaaacg atataaatat caataacatg gaaacacgct tggagaagaa aagttttgaa
    22381 atgatcgcat tgcaacagcc agttacaact gatttgcgta tgatcattac ggtcatgaaa
    22441 gctagctcgg atttagaacg tatggccgat catgcggtgt ctgttgctaa atccacgatt
    22501 cgcgtaaaag ggcagacgag aataccggaa atcgaaaaag agatctccga tatgtccgat
    22561 tatgtgaaaa aaatggtgga caacgtccta gttgcttatg tcaaaacaga cgaaaaagac
    22621 gcacgtacga tcgccaatat ggatcaaaga gtcaatgact atttcaatcg aatctataat
    22681 gcaacaatca aaaatatgca ggaaaatcct gagacagtca tcagtggaac agactacttg
    22741 aatgttgcca gttatctaga acggatcgga gactatgtga caaacatctg tgaatggatc
    22801 gtctacttgg ctacagggaa aatcactgag ttaaatacga accacaatga gattttctaa
    22861 actgaagctg tgattatgcc gtttgtttag gaaaagaggt tgtgaaatca gcggttgtga
    22921 aaaagctgtt ctgcatcaag taataagaac agtcaaacaa aaaatagctc ctcatatttt
    22981 tcgtttggct ggacttatta ccgcagatgc aagcttttga acaccgttta ttcgggaaag
    23041 ggtttgtgac aaaacatgtc acaaaccctt ttcatcattt catagaaagg ataaagaatg
    23101 caaaaaaaaa ttagcatcat cactatattt tttatttgtg tgcttacttc atgggttatt
    23161 cagcatcaag ccgctttata tgaagtacct gtcatcgaga tagaaaaagt acgaataaaa
    23221 gaggatcatc aattgattac aggaaaactg atgaacacgg atcaaaaagg agaatcgatt
    23281 tatttatcgg ttccttatca aaaaaatcaa atagaaacag caaatataaa agcgagacaa
    23341 atgtatctag tccaaatgca tggagatgaa tggcagctag tagcaaaaaa aaatgatggc
    23401 tggcttttct tcttcacaag tatcttcatt ggcgccatgt tgcttattgg tggaaaacag
    23461 ggtctatttg ctttaggaag tctcatcatc aatatcgttt tacttttact tttgttgctc
    23521 atctttacta aaacagaagg gatttctcta ttaaatatat ctatcctgtt cgtcgttata
    23581 gggatgatta ttagcatatt ggctttagat ggatggaata gaagcagtat aatcaaaatt
    23641 tcagctgcag ttacgagtgt ttttttagca ttttttattt gtcttttgac aatgaatcat
    23701 tggaaagacc aaggactgcg ctatgaggaa ctgaattatc tgacacgacc gtatcgttct
    23761 gtttttcttt ctagcgtact gattggaaca gctggaggaa cactcgatac cgttatcaca
    23821 gtcattgcga cattagaaga attgactaag cgacaaccag aaataacagc gaaacagtta
    23881 tggctttcag gaaggcaagt gggtcgagat gtgattggct cgatgaccaa tatccttttg
    23941 ttcgtctata tcagtgggtc agttccatcg cttcttttat atttaggaaa tcaatggtcg
    24001 ttcaaagaaa cgatagaaca gcatttatca ttggaatttt tacgcgtcat tgctggcagc
    24061 ttaggaatag tattatcgat ccctatctcg gtcctctttt ttctgactgt aaggaggctg
    24121 aaacgatgaa tgtacttatt atacttgttg ggattttagc aatcagcgtg ctacttattg
    24181 gcggtacgca aggtatgtat attcttttag gcttattcat caatttaggg atctttttcc
    24241 tgttgttatt cggttatcat cagaagtggc cgatactggc attgtcggtc ataggatttt
    24301 tattgattgc tgtggttatc ttatttttta tcaacggtta taatctaaaa atgcgtgcag
    24361 cttttgtgag tatactgatc tttttatttt gtttcctact attgcttcct atcactgatt
    24421 ttttagcaat ccaaggtttt acatcggtag aactagaaga attatctggc ttagataaga
    24481 ctttagcgat tgattttaga ttacttacga gatcgttgct tctgattagt cttagtggtg
    24541 ctgttttaga tgcgagtgta gcaatttcta gtgggacatt cgaagtatat caggcaaatc
    24601 cgcaattatc tttttaccaa ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg
    24661 cgtcaacagt aatgacactt ctttttgcat tcatgggaag ttctttagcg ttgatcctct
    24721 ggtttattga tttagacatt ccttttcagc aagtgatcaa tgaaaaatca tttgttctag
    24781 aatatacgat ggctattctt accacattgt ccgcactact tgttcttcct ttaacctgcg
    24841 tgactacagc atatctcttt acaaaaaaga aagaacaact caagatggag taaaagttac
    24901 aacttaagtc ctatgacatt tttactgaat cagtgcataa tatggatgta aggaaaagct
    24961 tgcaaacaat tttgcttaag gaggcaattt ccatgaaaga aagagaacgt attttagatt
    25021 tagtgaaaaa aggaatcctt tcgactgaag aaggcttaga tttattagaa agcatggcaa
    25081 ctgaaaaaga cgaaaagcaa atcaaaaaag aagcagacaa ggtaactgct agtcataaag
    25141 aaaaagatca ggcaagtcaa ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg
    25201 agccagttga tccaaaggaa agagaaagac aagatcaaga aaacttagaa aagatcttag
    25261 atgaactagc aactgaagcg aataaaactt cggcgcgtct agatgaagta aatgctcaat
    25321 tcgctgacaa taaggcagca cgcaatgaga aacaggaagc attgatgcag ctgaatacaa
    25381 aagaagaatt aggcgagttg acagaagaag aattagcaca gcgtcagacg ttagaagcag
    25441 aaatcaaaga attagaagct agtggcgata cactgttaga agaacaattg aaattagaag
    25501 cagaattaaa agatatccgt aagaatcaat ggtctgagaa aaaagaaacg tttactgata
    25561 agtttgaatt gccagatgat tggaaagatc aagcaacaga tacactgaat caagtaggcg
    25621 aaaaaatgtc agaagctgga tcacagttag gcaaattctt gaagaaaaca ttccaaactg
    25681 tttcagaaac agtgaatgac aacatggaat ggaaagatgt cagcttacgt gtccctggga
    25741 ttgcgacaac gaaatttgag catgaatttt actatgaagc tcctgcagca agtatccttg
    25801 atatcaaggc agcaaacgga aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag
    25861 ttgaagctaa gatcaaactt tacggaaaaa tgggtgctga accatttgaa gcattctctg
    25921 aacggagcca gattgaggtt aatgaagatc atatctcctt ccaaattcca aataaacgtg
    25981 tgcgtgcaga ccttgttttc tacttaccta aacgagtata cgaccacgca gctattaaac
    26041 ttctaaatgg caacatcatg attgaaacat tggaagctaa agatatttat acaaaatcta
    26101 cgaatggtaa catcatcgtg gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa
    26161 acggcaatat cgatattcga aacgggaaca tcttagattc tattatcgaa acagtaaatg
    26221 gtacagtgac atttggtgcg acagcagaaa acctttctgt gtctttggta aatggtgatg
    26281 tacgtcttac gattaaagaa gataatttga aaaaagtaga agcaagttca gtgaacggaa
    26341 atgtcaaagt cgctttacca gatggcatcg gtttggaagg acatgctaaa acaagtcttg
    26401 gaagtatcaa cagccgtctg tccaattacg aagtagttcg tgagaaaaaa gaacggacaa
    26461 accaaatgtt gcaattccgt cgtgtttcag atggtgaaat cgcgcaagtg caactttcaa
    26521 cgacaacagg aagtatctat ttaaaagata cagataaata aagaggttgt gaaagagatg
    26581 ttcagccttg agcaataaga agcgattttg taaaatagct tcccctattt tacaaaattg
    26641 tgccttattg ccgatagtct atctcttgaa caccgtttac caagagatta tgaacctgcc
    26701 aattatcgcc gagtcacaag gaataatttc agaaaaagct cgtcacgttt tatgaatatg
    26761 gtaatttgtg accgagaatt ttgcagaaag acaccgatta agaagaaggt tgtcacaacg
    26821 attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa agttgacaaa
    26881 atctaataaa aatgttgttt tgacagggac tttagcagga ataggagaat acatcgggat
    26941 tgatccgact gttatccgtg tgctttatgt attcctgagc ttagttgctt ttggaagtcc
    27001 aattatttta tacattttat tagctttgat tatcccatct gcgcgttctg cacgaacaaa
    27061 ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca
    27121 aacaaaaaaa cgcaaagaag cggaaaaaat cgatgatgac gaatggagtg acttttaatg
    27181 tcgtactttc aacgtatttt agttaatacg ttaacattca tatcattggc tgttcttttt
    27241 cctgcgatgg tccatgtgag cagtatttgg acggccgttc ttgccagctt cgtattgtct
    27301 gtgttgaata tgtttgtcaa accgattttg acgatactgt ccctgccctt tacattagtc
    27361 acattgggat tgtttacgtt tgttatcaat gcagcgattc ttaaaatgac ctcctttttt
    27421 gtgggagcag aacattttag tttttcttca ttcggtgcag cgttgttagt agctatcttg
    27481 atgtcgcttg tcaatgtaat tgtcagcgaa cataacagta gaaaataaaa gaaagctgtg
    27541 ccgaactatc ggcgcagttt ttttatttag aatcaggaat gcgttttcta ttacattggt
    27601 aattaaataa aaacgttttt tttaattggt agtacttttg gctagacaat gataaaatga
    27661 tagagaacac tctaatagaa atgaggtagc atgaatgtca gaagttgtaa aagttagcca
    27721 attagtgaaa aaattatctt tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt
    27781 catcgtaact ggggacatct cacgcccagg actagagttg actggttatt ttaattatta
    27841 ttcccataat cgtttacagc tatttggcag taaagaaatc agttttgcgg aacggatgat
    27901 cccagaagag cgtttgatca tcatgagaag attatgtagt gaagatatgc ccgcatttat
    27961 tatttcaaga ggattaccag ctccagaaga aatgatccag gcagcaaata aaaatgggat
    28021 agctgtactg cgttctccca tctcaacttc tcgtttatta ggagaattat ccagttattt
    28081 agatggtcgc cttgcccctc gaacaagtgt ccacggtgtt ttagtggatg tttacggatt
    28141 aggtgtactg attcaaggtg acagtgggat aggaaaaagt gaaacagcgt tggagttgat
    28201 caaacgcggc catcgattga tcgcagacga tcgagtagat gtttatcagc aagatgaatt
    28261 aacggtgatc ggagaaccgc ctaaaatcct ggaacatctg attgaaatcc gaggaatcgg
    28321 gatcattgat gtaatgaatc tttttggtgc gagtgcggta cgtggctcta tgcaagtcca
    28381 gttggctgtt tatctggaag catgggcaaa agataagaaa tacgaccgtc tgggctctga
    28441 agacacgacc gttgaaatcg cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac
    28501 aggacgaaac gttgcaatca tcattgaagt agccgcgatg aatttccgtg cacgtacaat
    28561 gggctttgac gcaacgaaaa catttgaaga aagattaagt cgcttgattg aagaaaactc
    28621 aggcaacgac tagcaaggag tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag
    28681 cttgtaggga ttcctatcta ctggtatgca ttgatcatcg tatcaggtat cattatcgct
    28741 atgtggctta gcagcagaga ggctgttcgg gtaggattaa aagcagatga tgtgacagat
    28801 tttatgttgt ggggattgcc tatctccatt attggcgctc gcttatatta tgttttattt
    28861 gatcttcccc agtatatagc tgatccaatc caaatattca atatccgttc aggaggctta
    28921 gctatttatg gcggactgat tgcaggcgcg atcacgctgt atttttttac taaatatcat
    28981 tttatttctt tgtggacttt tttagatatc gctgcaccaa gtgtattatt agcacaggca
    29041 atcggtcgtt ggggaaattt catgaaccac gaagcatatg gaccagacac gacgcgaagc
    29101 ttcttagaaa gtctgcattt accgcaattc atcattgata atatgtatat cgaagggatc
    29161 tatcgtcaac caacatttct ttatgaatca ctttggagtt tgattggatt tattttgctt
    29221 ttgtttttac gaaagaaacc acaccttttg aaaaaaggcg aagtcctttt atgttatcta
    29281 atgtggtata gtttcgggcg tttctttatt gaaggattac gtacagacag tctttacttt
    29341 ttaggtgtga tccgtatttc tcaattatta tctgctgtgt tatttgtcgg tacattgatt
    29401 ctatttattt ggcgcagaaa aaaaggaaat ttaccggatt atgaccgctc attaggaaaa
    29461 aatcaacaaa ttatctgatt tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa
    29521 aatgaattat agagtagcgg ttttaggacc aggctcttgg ggtactgctt tggcacaagt
    29581 cctcgccgaa aacggacatg acgttcggat ttggggacat cgtgcttcac aagtagaaga
    29641 aatcaacacc caacacacga atcaacgtta tttaccagat cattatctac ccgattccat
    29701 ccaagcattc accaatatgg aagatgcagt aaaagacgca gatgcggtgc tattcgtcat
    29761 tccaacgaaa gcaatacgtt ctgttgcaaa agaactagtg cctaaattga atacaaagcc
    29821 agtgatcatc catgcaagta aaggattaga acagggaaca cataaaagga tctctgacat
    29881 cttgttagag gaaatcccag cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag
    29941 ccatgcagaa gaagtagcaa aacatgacat cactacgatc acagctgcaa gtacagatgc
    30001 aaaagcagca aaatatgtac aagagctgtt catgaataat tattttcgga tctataccaa
    30061 tcctgatgtg atcggtgtag agatgggggc agctttgaaa aatattattg ctattggtgc
    30121 tggcgctatt cacggcttag ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg
    30181 attagctgag atcagccgtt taggtgtagc aatgggtgcc aacccgctga cctttatcgg
    30241 attaagcgga ataggcgatc tagtcgttac ttgcacaagt gttcattcta ggaactggcg
    30301 cgcaggcaac ttgttaggac aagggcagcc cctagaagag gtgctagcca atatgggaat
    30361 ggtcgttgaa ggtgtgaata ctacccaagc agcgatggaa ctagcccaaa tgatggaaat
    30421 cgaaatgccc atcactcaaa caatctatga agtgctgtat gaacaaaaag aaatcaaaac
    30481 agccgcaaaa gaaatcatgt tacgtgatgg aaaaaaagaa aatgaattct cattaacaat
    30541 tttttgaagg agcgaatgta tatgaaagtt aaaaaagcag ttattccagc agcaggatta
    30601 ggaacaagat ttttaccagc aacaaaagct atggcaaagg aaatgttgcc aattgttgat
    30661 aaaccaacaa tccagttcat cgttgaagaa gcgttgaaat caggtatcga agacatcttg
    30721 atcgttacag gaaaagctaa acgaccaatc gaagatcact ttgattcaaa tattgaatta
    30781 gaaacaaacc taagtgaaaa aggaaagacc gaattattga aattagtaga agaaacgaca
    30841 gatgtcaact tgcatttcat ccgtcaatct catccaaagg gtttaggaca tgccgttctt
    30901 caagcaagag cctttattgg aaatgaacca tttgtcgtta tgctaggcga tgatataatg
    30961 gaagacgaag taccattgac acagcaatta atcgatgatt acgaacgtac tcatgcttct
    31021 acgatcgccg ttatgcaagt tcctcatgat gaaacttcaa aatacggaat tatcaatcca
    31081 ggagaagtat tggaagatgg gttatacaat gtcaaaaact ttgttgaaaa accagatcca
    31141 agcaaagcac caagtgattt agctattatt ggacgttact tattgactcc agaaattttt
    31201 gatgtattgt ccgagcaaca accaggagcc ggaaatgaaa tccaactaac tgacgcaatc
    31261 gatacattga ataaaacaca acgcgtattt gcccgtaaat tcacaggtaa acgttacgat
    31321 gtcggtgaca aatttggttt tatgaaaaca agtattgaat atggtctaac acatccagaa
    31381 gtaggtgcac ctttacgtga gtacatcctg tctttaggta aaacattaga agcagaagga
    31441 agcaagatcg atcataatcc aaaaggaata acagaaatca aagaagaaaa gaaaaaataa
    31501 cccttttttc taacgagctt cagctgctaa ttgaataaaa agtagttgaa gctcgttttt
    31561 atttgtttaa aaaacacgaa aatgctatcc ttatagaaaa gagagacaag aagggagttt
    31621 gatcatgtca ggaggagaaa ttgcaggatt gatcgcagca atcgcctttg cgatacttgt
    31681 cgtttttatc gttcgtgtgt tgttgcaagt ttctaaaact ttagaaaaag tagatcagac
    31741 tgtagcagaa gcaaatacga cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag
    31801 acaagtagaa ggcttactgg tcaaaagtaa tgaactgctc ttggatatca atggaaaagt
    31861 cgcaacgatc gatccattgt tcacagcagt tgctgattta agtgaaagtg tatcagaact
    31921 taatcattct agtaaaaaca tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa
    31981 agcgacggtt gccggaaaag tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca
    32041 tactgaaacg acaggaggaa aataaaaatg gctaaaaaag gtggattttt attaggtgca
    32101 gtagtcggag gcacagccgc agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa
    32161 gaacttcggg atgaattagc tgcccaagcg gatgatttga tggatatggc ttcagattac
    32221 acagatatgg cgaaagaaaa atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa
    32281 gatctttctc aacaagcagg tgatctagca ggtagtgtca aacaaaaagt aagtgaatca
    32341 gcagatgaag caggaagtga atctgacgat atcttgttaa atcttaaaga acaatcagct
    32401 gacttatcta atcgtttcaa aaaaacggca gaagatatga aaaaacctgc agatgaatta
    32461 ggaagtattg ctaaagatac ttcagaagat atcatgattg atgtaaaaga cgctgctggt
    32521 aaagcaggag atactgtcaa tgaaggaatg tcagaagcaa aaccagttgc agatgactca
    32581 aaagaaatga tgaaaatggc aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat
    32641 acaatgacag attcatctac aaatcactaa aatcacaaat ttatagaata aaaaggtggg
    32701 agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat tcttttggtg
    32761 tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg atacgtaccc
    32821 ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct tcttcgatta
    32881 ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg atcccgtgac
    32941 ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt cgtgcgattt
    33001 gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca agctgtgctt
    33061 caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa gcaattgtac
    33121 gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac acaagttcat
    33181 tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt gctcctgcaa
    33241 ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg tattcaattt
    33301 ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca atctcaaatg
    33361 caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga gttttgatca
    33421 gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc gtcaagatat
    33481 cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct acattgccag
    33541 gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt tcgctgtcta
    33601 aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact tccaaagcag
    33661 gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca cgctcatgag
    33721 gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata taagccaatt
    33781 cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta ttcatttggc
    33841 aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta ttaaaagacc
    33901 aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa aacggttgca
    33961 attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc agaaaacata
    34021 taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta tgatgtagcg
    34081 agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa tccaaacgtt
    34141 aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta tcgtccaaac
    34201 gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat catccctgat
    34261 gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc aacaatgtac
    34321 aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt caatgtattg
    34381 aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca tatcacagac
    34441 gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg ctctattgat
    34501 ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa agatgcagta
    34561 aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc gttgatcgat
    34621 cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga aaataaccta
    34681 ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg tctatctcta
    34741 gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga tgaattagcc
    34801 atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga attcgaaatc
    34861 atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac aagtatcaca
    34921 caaccattat acgatttagg ggcggtatct atgcgcctat tgacgaaaat gatgaataaa
    34981 gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa aggatctaca
    35041 aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc tgcgttacaa
    35101 ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg tgattcggag
    35161 ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt gttataatcc
    35221 tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg tatataaaac
    35281 aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg gactgtgaca
    35341 atactattgt cacaatccct tattactgtg tattctatta aatcaatcat ctttctcatc
    35401 tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc agtaatctgt
    35461 atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg gtccattttt
    35521 cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat tgacagtaac
    35581 ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat cagatgataa
    35641 ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt gattgatcag
    35701 attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag tattgtcatc
    35761 gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc atgaatctcg
    35821 gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat ttccgtttaa
    35881 gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac tacgcatcat
    35941 gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc tttcgtgttt
    36001 ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt cttcatagac
    36061 gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga catctactgt
    36121 acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca tctttgccaa
    36181 gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt gatataagtg
    36241 ataagccggg atattgttgg accattctag tgactcacgg acggtttgga aggtgttcga
    36301 tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt gccaagttgt
    36361 tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg caggtccata
    36421 aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg catggttatt
    36481 ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag ttctgttatc
    36541 catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca tgtatccata
    36601 attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg aatggattgt
    36661 atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac tgaccgatga
    36721 atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc ttgttgcttc
    36781 attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt gttgtggtag
    36841 aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg taatttggcc
    36901 ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag aaagatcttc
    36961 ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg gtaatccagc
    37021 gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga ttccttttgc
    37081 agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg gtgagacatt
    37141 caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg cagaaagaat
    37201 gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct gtttgactaa
    37261 ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag atactaatgc
    37321 tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat cctctgtgct
    37381 gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg tgcgcatcag
    37441 atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg acgtttctgt
    37501 aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt cttcgacgag
    37561 ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca aaagaagaac
    37621 agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa caaaccattt
    37681 ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa gggtcacctc
    37741 aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac agacaaggct
    37801 gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga tctttattat
    37861 aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga acagggcaaa
    37921 gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt gttagtaaca
    37981 attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc atgagcggaa
    38041 agaaaatgct gttaggagca gcagaagaag cagccagtta tggatcttcg acttttatga
    38101 tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg aatattgatg
    38161 ccggaaaagc gtacatggca gaaaaaggtc taacaaatat cgtcgttcat gccccttata
    38221 tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc caatttctga
    38281 gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt cacccaggtg
    38341 ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc ttgaatgaag
    38401 ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt aaaggtacgg
    38461 aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa ttgaatgaaa
    38521 aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat attaaagaag
    38581 acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg cttaaagtac
    38641 tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac gccaatattg
    38701 gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca atgtttgaca
    38761 atatttcgaa aatcttggaa actccctatg taggcaaaga taaaaagaaa gcttacgcac
    38821 catatggaaa agaaatcgcc atgttcaaag cgcaaacatt cgatccagaa gtgtttcctg
    38881 agttattagc agaagaagtg atactctaag attttctatc tgacagcagc aaaaaaggac
    38941 caggcacatt gccttgtcct tttttgttta agaaacaaag gagtactttt tgcagtcttt
    39001 ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacaaggta tactggtaga
    39061 aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga aggacgaaaa
    39121 gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata catactttat
    39181 tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta aagcaatcta
    39241 tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg ctcaaaatat
    39301 tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag gaatctccta
    39361 cagcttattt ttagtacttg cacataaact gcatctttct tatgcagttt tgttcggcat
    39421 gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgattg tgaagaacaa
    39481 gtatcttctt ttaagtatct atttttattt tttgttttct ccgattagtt ttacacatga
    39541 atattccact cgaacatatc gtaatgccct cgtgattccg gctgttgaga tcattatcgc
    39601 ttgtttatta gctatttatt accgcaaata cggttctagc agaaaattgc tgccatggtt
    39661 tttagggctt tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt ctatctggct
    39721 tcttcctttt actgtagtag ctttgggtat cagtttcgtc cagatcttgc tagggcaaga
    39781 cttttctttc aaagaagtaa agaagttaaa aaaacgactc tttcttctct cgaaacggga
    39841 atggctgaaa ctttcattgt ttttacttcc tatagttggc ttttccatcg tctatcaatg
    39901 gatcgaatat aaaaatgaga caacatacgg tattgcaacc atcaatgacc gttccagtgg
    39961 agaatttgga cgtttgatga aacaactgat ccgaatagat gatgaaacga acttgaataa
    40021 agaaaacagt gagatatggg tctcacatgc tgcactaaat aaagcattag ccgcatctcc
    40081 tacattccat actgtttcta cccgtattga cgaattatat acgactcacg cgtggactca
    40141 aggtggaaaa gtaaaagaat tacccggaga tatcatcttt tgggcattgc gagatgtggt
    40201 aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt ggaaaaaagt
    40261 caaccaagaa ttagaagaag cctatcacaa gggaacaatc aaaaagaaaa aagagttcta
    40321 tctgatggga tcaggagatg gaaagacgat gagtgatctg ccaatcgtat tcgatttcct
    40381 tcaatcagga tacgcttatg catggcgata taaagatttc catcaaggag gaaattatag
    40441 tttcggaacg caagaacaag tcaaacaggc tcaagaatta ttaaaccttc cgctaatgga
    40501 gaattggatc gatagtaatg aaccgagaac acctaatttc caattaacaa aatcagcaaa
    40561 agtcgcgaat gtcatcataa aaatttacca agttctggct attcccgtgc tgattgcttc
    40621 aatagcaggg atattactta tgatactagg aaccgtattt gcaaaagctg atcgtgccta
    40681 ttatagttcc tctttgattg tggtcaccgg attaatactg acacaattgg tttttctttt
    40741 cggtgtctca tggttttgtt cttatgcgcc agaaaaaaag gactattttt taagtgtcta
    40801 cactggtgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc tagttttcag
    40861 taaattaaat ctgaaaaaag ctttatcttc taaataaaaa gacccttggt agctagatt
//
LOCUS       DAPBCS010000025        37431 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPBCS010000025 DAPBCS010000000
VERSION     DAPBCS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.025"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..179
                     /locus_tag="RD521_001488"
                     /pseudo
     CDS             1..179
                     /locus_tag="RD521_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface adhesion protein"
     gene            243..1025
                     /locus_tag="RD521_001489"
     CDS             243..1025
                     /locus_tag="RD521_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7771230.1"
                     /translation="MEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVF
                     ELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQ
                     TAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLA
                     IDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNN
                     PTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1449..1619)
                     /locus_tag="RD521_001490"
     CDS             complement(1449..1619)
                     /locus_tag="RD521_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7771231.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1813..1992)
                     /locus_tag="RD521_001491"
     CDS             complement(1813..1992)
                     /locus_tag="RD521_001491"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771232.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(2002..2178)
                     /locus_tag="RD521_001492"
     CDS             complement(2002..2178)
                     /locus_tag="RD521_001492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771233.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            2379..3491
                     /locus_tag="RD521_001493"
     CDS             2379..3491
                     /locus_tag="RD521_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7771234.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3569..3862)
                     /locus_tag="RD521_001494"
     CDS             complement(3569..3862)
                     /locus_tag="RD521_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDT7771235.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            4030..4344
                     /locus_tag="RD521_001495"
     CDS             4030..4344
                     /locus_tag="RD521_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDT7771236.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4365..6344
                     /locus_tag="RD521_001496"
     CDS             4365..6344
                     /locus_tag="RD521_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7771237.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6357..6683
                     /locus_tag="RD521_001497"
     CDS             6357..6683
                     /locus_tag="RD521_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDT7771238.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6699..7217
                     /locus_tag="RD521_001498"
     CDS             6699..7217
                     /locus_tag="RD521_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771239.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7217..8569
                     /gene="celB"
                     /locus_tag="RD521_001499"
     CDS             7217..8569
                     /gene="celB"
                     /locus_tag="RD521_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDT7771240.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8653..10158)
                     /locus_tag="RD521_001500"
     CDS             complement(8653..10158)
                     /locus_tag="RD521_001500"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDT7771241.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10250..10840)
                     /locus_tag="RD521_001501"
     CDS             complement(10250..10840)
                     /locus_tag="RD521_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7771242.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            11019..12665
                     /locus_tag="RD521_001502"
     CDS             11019..12665
                     /locus_tag="RD521_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7771243.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGIFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12714..13433
                     /locus_tag="RD521_001503"
     CDS             12714..13433
                     /locus_tag="RD521_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDT7771244.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13912..14859
                     /locus_tag="RD521_001504"
     CDS             13912..14859
                     /locus_tag="RD521_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7771245.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14871..15791
                     /locus_tag="RD521_001505"
     CDS             14871..15791
                     /locus_tag="RD521_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771246.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15832..17289
                     /locus_tag="RD521_001506"
     CDS             15832..17289
                     /locus_tag="RD521_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDT7771247.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17634..18248
                     /locus_tag="RD521_001507"
     CDS             17634..18248
                     /locus_tag="RD521_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7771248.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18241..19965
                     /locus_tag="RD521_001508"
     CDS             18241..19965
                     /locus_tag="RD521_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7771249.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19962..21413
                     /locus_tag="RD521_001509"
     CDS             19962..21413
                     /locus_tag="RD521_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771250.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21886..24039)
                     /locus_tag="RD521_001510"
     CDS             complement(21886..24039)
                     /locus_tag="RD521_001510"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDT7771251.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(24041..25087)
                     /locus_tag="RD521_001511"
     CDS             complement(24041..25087)
                     /locus_tag="RD521_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771252.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25237..26526
                     /locus_tag="RD521_001512"
     CDS             25237..26526
                     /locus_tag="RD521_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDT7771253.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26539..29241
                     /locus_tag="RD521_001513"
     CDS             26539..29241
                     /locus_tag="RD521_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDT7771254.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29226..30083
                     /locus_tag="RD521_001514"
     CDS             29226..30083
                     /locus_tag="RD521_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7771255.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30154..31053)
                     /locus_tag="RD521_001515"
     CDS             complement(30154..31053)
                     /locus_tag="RD521_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDT7771256.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31222..33393
                     /locus_tag="RD521_001516"
     CDS             31222..33393
                     /locus_tag="RD521_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDT7771257.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33461..33802
                     /locus_tag="RD521_001517"
     CDS             33461..33802
                     /locus_tag="RD521_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDT7771258.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(34078..35832)
                     /locus_tag="RD521_001518"
     CDS             complement(34078..35832)
                     /locus_tag="RD521_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771259.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35843..36679)
                     /locus_tag="RD521_001519"
     CDS             complement(35843..36679)
                     /locus_tag="RD521_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDT7771260.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36681..37364)
                     /locus_tag="RD521_001520"
     CDS             complement(36681..37364)
                     /locus_tag="RD521_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDT7771261.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12080 a         6588 c         7689 g        11074 t
ORIGIN      
        1 gtagaattaa cgaaagaaaa ccgacttaca ccaggcggtg tcgtgttgac aaagatcgat
       61 gaccaaagcg gggaaatcct gcaggagctg tttttgagtt acagaaccga gaaggagaaa
      121 cgctacaaac cggtctaact acaggagaag atggaaaact ggcaattgac ggtttagcac
      181 caggagcata tcagctggta gaaactcaag cgccaacggg gtatgaatta gatgcgacac
      241 cgatcgagtt tgagatcgaa agaagtcaga cagcggtggt agaattgacg aaagcaaacc
      301 gacttacacc aggcggtgtc gtgttgacaa agatcgatga ccaaagcggg gaagtcctgc
      361 aaggagctgt ttttgagtta caagatgcca atggaaccgt tctgcagtca ggtctaacca
      421 caggagcaga tggaaaactg gcgattgacg gcttagcacc aggagtgtat cagctggtag
      481 aaacccaagc gccaacgggg tatgaattgg atgcgacacc aatcgagttt gagatcgaaa
      541 gaagtcagac agcggtggta gaattgacga aagaaaatcg acttacacca ggcggtgtcg
      601 tgttgacaaa gatcgatgac caaagcggag aagtcctgca aggagctgtt tttgagttac
      661 aagatgccaa tggaaccgtt ctgcagtcag gtctaaccac aggagcagat ggaaaactgg
      721 cgatagatgg cttagcacca ggagcatatc agctggtaga aactcaagcg ccaacggggt
      781 atgaattgga tgcgacacca gtaactttca agatcgaaaa agaacaagaa gtggcgatct
      841 ttttaacaaa agagaataga aaagttgctg atagctcaga tataagagga aacaatccaa
      901 caagcagtgg aaataagcat cttccaaaaa ctggagaaac catcattagc cagtttatat
      961 tgtcaatatt gggtgtgcta cttgttttca tcagtataaa atttagaaag aaacgaaata
     1021 tatgagctaa aataaaaaat tgaataagca tagtttttaa aatcaactag tacccatcaa
     1081 ttgaatggaa actaccgatt ttagaagggg tatacaaaca aatattagta tcggagtaga
     1141 gaaggttgaa cccaaccagt aaataagtaa agacaatttt gtgctgcgtt gagaagtgaa
     1201 aaataaacct ttgtagtaat actagttaat aaaaaatata caaaaacact gcgaaaatca
     1261 ttcttgagtt tcgcagtgtt ttttgcctaa tgactcaagt ttattgggta atactttgat
     1321 tttctttcta caaataatca gcgatcagtt gtaatttttt ctctagtttt aatggctctt
     1381 tgtcaataag gagaaaaaca agtcgggtct tttgtctcgg aaagcttcaa ccaactcgtg
     1441 aataaaggtt acgcctcctt gaaaggcgtg gaaaaaccaa gtaatcgatc aatcaccaat
     1501 ttcgcatttg gtagaacatg tttggtgagc tgaaagtagg tggcgttcat gtctgtaact
     1561 aagaattcta tttctttagg attagtgcag ctaaggaaat agtttgttaa tcgaggaatt
     1621 ttttgtgtag gtaaattttc tcgattaatt tttctgtctc gccatccgcg gaaataaaac
     1681 tcagtttatc ctcaacggaa acgtgagaac gaaattcaca accctcaaca ctcaagacag
     1741 aattcttttg gaagactttg gtaaatagct tctcaactct ttcaatgtgc gaataacggt
     1801 tgtcaaagat acctaacaac ttttcgaaat aaaaaataac gacacttttt cagtcagtaa
     1861 agaagtgatt ttatgtctga catgatttga aatcgagtgc cttggacgga taaaataact
     1921 ttgagcgttc caatggcttc gacagttttt acaggtatag cgctgctttt ttaggcgtat
     1981 aaccattggc atatgattat attattcaaa gtggacaatg gtttccttct tcttgttttt
     2041 caccaccatt aatttcctat ttccatctac cacagcagaa ccacaatttc tacagacaca
     2101 tggagcaggg aagagaactg cagcaacgat caatgttttt ttcttctgta aggtttcgta
     2161 agagacctct gtaatcatca aatttttgtc tattgatctc agaatttttt tgatagaacc
     2221 attcataaag catatcgttc tctcagttgt ttattttgtg gtgatttaat catacaagaa
     2281 aacgatatgt ttttttataa aaacatgaat aggaaaaata gttgacaaga aaatgacaac
     2341 gccatatgat gttccatagt atttacaacc tgtcatagat gactgtaaaa aataaaagta
     2401 aaaagaaagg acgacaacag gtggattttt atcagtctct acaactagat ccgtttattt
     2461 taaaacaaaa aatacgagaa gcagcttcta aaaaagaaaa gtgcatgtat atttgttcat
     2521 tgtttttacg atcattattc atcgtattgt ttgccatttg ttttatcatt ataattacaa
     2581 cattatttga atcgaagcat aaaccatacg ctgtggtttt attctgtatg ttgatgagta
     2641 ttcgttttgt tgactttggt tataagattt cccattcgat cataggttta gcaatcgtca
     2701 tgttctcact attagtagca ccgtatgtcc agctgatcaa atggtcagtt atgggcatgt
     2761 tgattcattt tattttacta agcagcatat taatggcaac cgctagtgat cccaaaatgg
     2821 ggaatgcttc tttgtacggc ttttcatatc tctttattgt ttattcgtta cctaaagatt
     2881 cgttgaacaa gaatttcttt acacaaacaa gtagcttatt atttttgttt ttttgttggt
     2941 tttcagttct tctttacaga aaacatcgag aaaagaacaa agacaaaagc ttgtttaaag
     3001 agattttttt gaaaggcatg tattcgcagg aaaaaatttg gatgctcatc tatgcatttg
     3061 gcattagtct tttgatcgtt gcaggagagt atgtcccttt tcagcgattg atgtgggcag
     3121 ggtttgcatt ttcttctatt gtatctagct atggattgat gtcgattggt ttcaaagaac
     3181 gagcagtaga ccgaatcatt ggctcattga ttggatgtgt tttatttatt ggtatcagcc
     3241 aatttattcc ttttaactgg gtcggtatat taggtggtct agctttaggt gtttgttcta
     3301 cctatagata taaaacagtt tttaattctt ttggtgcttt ggcaatcact gccagtttat
     3361 tcggggttcc aggagcagta acgatccgca tttttgagaa tatccttggg gtatgtttag
     3421 gaatcatgta tattggggtg actgaaatac ttatccgaaa aataagagaa aaacacggtt
     3481 tgaatcatta gatgagatcg aataaaaaat aaggaaaaag tgaagataca catggatgat
     3541 aaattgctat cattccatgt gtatcttttt acggtaaatc agtgaaacgt tcgactttca
     3601 tttttaattt atacttttta cggagttcgt ttatttgagc catcatttcg gatttgtgat
     3661 gcttttcgat tgcttcttca tctttccaac ggtcgatcag taaaagagat tctggatctt
     3721 ctagtggaaa gtaatagtca tatttctcgt ttccttcttc tgcacgaatc gctttgacaa
     3781 tgtttctttc gaccatttct tctgcaaatt ttctggcttg tccatttgta cttgtataca
     3841 aaatattcac tgtgatactc actaaatccc ctcctgaaaa aattctatca ctagtatatc
     3901 acggtgccag aaattattat tcaagatatg atacacattt tttccgcaat gctacggaaa
     3961 tatatatttt gaatagaaaa cgcttacaag atatgcttgg tttataaaaa taaaaggagt
     4021 gattgattta tgaaaaaagc attgattatt tgtgcggcag gaatgtcatc ctcgatgatg
     4081 gcctcaaaga caacggattt ctttaagcaa aaaagagaag aggtactcgt agatgcagtt
     4141 tcagctacag aaggcgataa tatgattaag aatagtgatt ttgatttatt tctgatcagc
     4201 ccgcaaacga cgatgttttt ggataaattc aaaaaattag gagaatctgt tggaaagcct
     4261 gttgttagta tccctttcca agcgtatgtc ccaatcccta caggcatcca gaagttagca
     4321 gaggtgatcg aagagaacat atgatggaat aggagtggat gataatgaaa cagaaggaaa
     4381 aattgctgtt gagatatttg attgatcaca agaaggagta tgtcaccagt caacgtttag
     4441 cttctgaatt gttattatct gatcggacaa tccgtaatta cttgcagcgg atcaaagagt
     4501 tagtagaaaa aaacggaggt aagatcattg ctaaaccagg ttatggctac caactgcata
     4561 ttttacaaag actgacattt gacttgtttt tgagtcggca agaaatagta cctggatatg
     4621 ctagagaacc gcaaggattt tatgagtccg aagatcgaca aaagtatata ttgaaccaat
     4681 tgcttttgga agatgccatt ctttttatgg atgatttggc agaagagctt tatatcagtc
     4741 gttccagttt gacaaaggac atgcaagaaa tcaaggagcg attgattcca tattcactaa
     4801 aaatcgtttc taagcatggt caagggacat ggatcgaagg ggaagaaaga aatcgacggc
     4861 attttatcat ggatactttt tttggaaacc attatggaaa ttcattgaaa gaatatttag
     4921 ggcaaagccg ttttttccca gacatcagtt ttgaagagct agtcattatc attctcgatg
     4981 aaacaagaga agctaagtta aaagtttcgg attttatcat acagaatctg actttacact
     5041 tggctttggg gatcaaacgg ctccgagaag ggtttgaaat caaggaatta ggcatcgaac
     5101 aagaagtttc taagcgagta gagtatcaag tggcgcagcg gatcgttcaa cgtattgagg
     5161 taatcgcagg tgtatcgttt cccgaagaag aaattgctta tttgacatta catctcatgg
     5221 ctaagtcgaa tcacaaagaa aatcaaaatg atcaggaact atcaaaggaa ttagcgaaag
     5281 ttctgaaaag attgtctcag gaatttcatt tttcattagt agaggatctc cagcttttca
     5341 atggactact cgatcatttg tcaccgatga tgattcgatt gagccgaggt attcgcattg
     5401 agaacccatt gactgaggag ataaagaaag aaaatcccaa agtctttgac gcagtaaaaa
     5461 ggcatttcag caacatgccg gctttgaaaa actataccat caatgaagat gaatgggcct
     5521 atttggcttt gcatctcatg gcggcattgg aaaaagaaag agctgcgcat aaactacatg
     5581 cattgattat ctgtgcaacg ggttacggca gtgcccaact attaaagaat cgcgtagtca
     5641 gtgaattcgg taaaaatatt acagtggtaa gtgtaaaagg ctattatgag atcaatgaaa
     5701 caactttaaa aggaatcgac ctgattattt cttctattga tctttcgaca atgtttttca
     5761 aaattccagt tttacatgtc agtatttttt tgaatgaaga tgatgttcgt aaaatacgaa
     5821 aggtagttgg agaatctata ccctatcatt cctcagatag accgatacca gcattttctc
     5881 tacagcaaaa aaagcagatt tacaccgaac agttgtctga aaaatttttc aagacttata
     5941 cgtacgaacc gaaaaaggaa gaaattcttc atgatttgct gcaaaagctg agcataaatg
     6001 aagaagaagg atatataaga gaaatgcgaa agcagatcca gcaacgtgaa aatatgggac
     6061 aaatcatatt cagtgataca gtggcagtcc cacatcccgc tgttccagtt ggaatcgaaa
     6121 caaaggtagc cgttgcattg attccttctg ggatgcagtg ggatcaatat caagaaattc
     6181 attttgtttt tttagtttcg ccttcttatg tcgaaaatga aggaattacg gtcgtgacta
     6241 aagcaatcgt taggttagtt gaccagctaa aagtacagca ggaaatctta gctgaaccta
     6301 tttttgagaa tttcagcagt aaatttatca aactgatcga ataaggagga aaacagatgg
     6361 ctgaaaaaat aaacagtgaa acactacaag tgacttcttt tgatatcatt ttccatagcg
     6421 gaaatgcacg gacactgata catgaagcat ttcgctcgat gcgtaaagag atgtttgaag
     6481 aagtagaaga gaagttgcga caagcaaatg aagagattct tcatgcgcat cagtcacaaa
     6541 ctcaacttct gaaagaatat gccagcggga caaaaataga aatggaaatt atcatggtcc
     6601 atgcacaaga tcatctgatg acgacgatga cattattgga agttgcacag gaaatgaatt
     6661 atttgtatca aaaaaataaa tagaaaggaa ggatcattgt gcaggataca gtaaaaagga
     6721 aaatcgaatt agaaaaaaag caacttagtt tgaaaaacat gtatttcaat cgttatctaa
     6781 tcgtacgtta cctgactgcc tttttctttt tcatgaacat gcaatggatg attctgcttc
     6841 tatcagctaa aagtttagga agtagtttac cgatgattct gattttagct attttgcctg
     6901 cagtaggaga acaagtaaag ctttatcgaa aacaccaaac gaacgttcca tggacaaagt
     6961 ggtatttttt gttccagagt gtctttaatt tattagtgat tccagtgttg tttacctcag
     7021 gatttacatt cttgtatcct tttatggcaa ataacaatgg aggacagtta tttgttttca
     7081 ttcttgttgt aagcggtatt tttgccagtg catttataca gtatcgttta aagaaaattt
     7141 cactcaacca agaccaacag tatatccgta ttaaacaata tgaaaaagcg ttatatttag
     7201 gaaaggagaa taactaatgt ttgatttttt gcagaaatac ctgatgggac ctatggggaa
     7261 aattgcgcaa cttaaaatcg ttcgggctgt catggcagcg gggatggctt ctattccttt
     7321 cacgattgta ggctcgatgt ttttggtttt aaatattttg ccattaccgt tcccattttt
     7381 agaaggattt tttaatgcaa cttttttcaa agttagtgac ttgtacatga tcgtcaatac
     7441 aatgacgatg gggattttat ctatttactt tgccattgtg tttgcttatg aattaacgtc
     7501 aattgaacga gatgaacaaa aattaaatgt taatccgctc acgggggcat tactatctgt
     7561 ttttgcattc tttatgtgta tccctgaatt gatcatgact gatggtaaaa tcagcttgat
     7621 tgcttctatc aatgagacag aaactgtagt aaatggtatt aggatgggct cttttgtcga
     7681 acgtttagga acttcaggaa tctttacagc tattattatg tcgattattg cggtcgaatt
     7741 gtattgtatg tgtgtcaaga gaaattgggt cataaaaatg ccagatgttg ttccgccagg
     7801 agtttctcgt tctttcactg ctctgatccc aacatttgtc attgcgtttg tggtgatgat
     7861 catcaatgga ttattagttg cactaggaac ggatatcttc aaaatgattg ctatcccatt
     7921 tgggtttgtt acgcagttga cgaatacatg gcttggtgtc atggtgattt atttcttgat
     7981 ccatgcattg tggatcgtag ggatccatgg agcgaatatc atcacttctt tcttgacccc
     8041 gatcgtatta gctaatatgg cagctaacgc acaaggcgca aattatccgt tggcaggaga
     8101 attcaacaat tcttatgtaa ccgttggtgg atcaggtgcg actttaggat tgatcatctt
     8161 tattgcattc ctcgcgaaat ctgatcagct gaaagtgtta ggaaaagcct cacttgtacc
     8221 aggattattc aatatcaatg aaccaatcat tttcgggatg ccaatcgtct acaatcccta
     8281 tttagcggtt ccgtttttct tagctccgat ggcttctgct acactcgctt attttgccat
     8341 tcggctggaa atcgtcaaac cgatgcttgc acagatgccg tggccttcac cagtcgggat
     8401 cggagcattt gttggtagtg gtggtgattg gaaagctgct gtgttagcag tcatttgtgc
     8461 aatcatcgca ttcattatct ggttcccatt tatcaaattc tatgataata aattattaaa
     8521 tgaagaaaga gaaaaggcag cagaattagc tagtgaaaag ttaaaatgat ttctaagaaa
     8581 aaaggaactg tgacaaaaaa tcacagtccc tttttttctt agaaatggga aaccactagt
     8641 atataaatac cattattctt caatcaaccg cttcacgatt gcataatccc cttcataagg
     8701 ttcatctaca ccattgattt tttgataggg atccacgcct tttcccgcaa taacgactga
     8761 atcttgcgga gcgctttctg ctaaagcgcg tcgaatcgct tcaggacgat ccatttcata
     8821 atgaacgatc aaatctggat tagtaattgc ttcattgatt tcttccgcga tttttttcgg
     8881 atcttcaaat gctgggtcat ctgccgtcaa aaaggcgatg tcagctgttt ctgatagcac
     8941 ttttccaaaa tcatgtcgac gggagattgc tttgtttcct gggctgccta atacaacaat
     9001 tacacggcca tctggatgtt catttttggc aaattccagc aatgttttca agcttagata
     9061 attatgtgca tagtccacat agacatgcgc tccattggat tggatcaatt gatccattcg
     9121 gcctggtaca cgagcttctt ttaatccgtc ttgtgcatct tgactggagg cacctgttag
     9181 tgctgcggca atgatcgcac tggctgcatt ttcttggttg aatcctccag ccagcaaaat
     9241 atcataagca gtatcgcaga tagctaagcg atcttcatga gaagataaag taaaaccgtg
     9301 agtaccttta tctgaacgga tcacttgata attgctttct tccgaacgac cataagtgat
     9361 cgttggaatc tggaatagat cggctgtttc cctaagtaaa tcaaaatagt cactttcatg
     9421 atttaaaaca actgtttttg aattcaagat caattgtcgc ttgcaataga aatagtcatc
     9481 aaatgtcgga tgttcgatcg gactgatatg gtcaggcgag atattcaaga agattccgac
     9541 atctaaagtc aagccgtata cgcgttgtgt tttgtaagct tgtgaagaaa cttccatcac
     9601 tagatgagtc ataccatttt caactgcttc tgacatcata cgatataaat ctaatgactc
     9661 aggcgttgtc aaatgggatt tgaaatatgt tttgccatct aacgttgtat tcatagtaga
     9721 aagcaatgct gtttttttat ttgttgtgtg atccaagatt gcttttgtaa agtacgcagc
     9781 agttgtttta ccttttgttc cagtgaatcc gatcactttc aatttatttt gcggatagtc
     9841 gtaaaaagcc atagcaagaa gcgccattgc tttacggata tccgttacga tgattcctac
     9901 tgctgaattt tcgtatggtt gttctgatac ataatattga ataccctgtt cgattgctga
     9961 atccaaatat tcttctttga aattcattcc tttacagaaa aacaaagtag aagcatcagc
    10021 ttttcttgaa tcataactta actgaagaaa ttcagctttt tctggtacat caagatgcca
    10081 gcctcttgtt gaaacaaatt cttttaatag attttctttt aatagtaaat gacggatgtc
    10141 ttctaaagag aagctcattg attgatccct cgctttattc aatttcttgg tttattatag
    10201 cacactcttt ttaatatatc gtttgtttcc gctatgacaa aatccagctt tattcaaatt
    10261 ttcttgacac aggggacaag cggatgagta ttttcataaa caatgaactg actgtaatag
    10321 ataggagaac gaatagtgtc tccaacaatc caaaaacata ataattcaaa aagacgataa
    10381 gactattggt aagtaaaagt ccttgcggca ttggaagccg aaaaaatttg tggaaaataa
    10441 tcggcggtat cgttataccg ccatttgagg ccccgactgt gtacaatgtg taaaccccaa
    10501 gactgaacaa aagacttcct gaaatcagtg aaaagaaatg tgaggagatc atttccactt
    10561 ttggaacaat ctctaaaaca acaggaaaaa gcagacttcc cgctacagtc ttaaaaaata
    10621 ctgattttcc taataggaca gcggctagaa gcagcatcat aagattgatg ctccaaacag
    10681 taactgagcg ttggatagaa aaggccgttt caactaaatg tccgatggcg cccactccgg
    10741 cggaagctat atcgtgaggg agcaagaacc aattcaatcc aactgtgata aagaaaagac
    10801 ctaaaaatat aacgggtagt ttttttattt cttgtgtcat tgatatctcc tataaatgtt
    10861 agccataaaa ggctgtatat ttctttactt taccaaaaaa acgaaaccga attcaaaaaa
    10921 acaattgttc ttataaaagt tatataaaat cgattgccag tttctgtttt accaattaaa
    10981 atgatagtaa tgttttgtag aaagttggaa tctgaccgat ggataatcaa agaaaaccag
    11041 cattagatca attaacaccg caagaaaaaa tggcacgagg ctctgcttgg atgaccgcca
    11101 gtaatatcat ttcaagattg ctaggcgctg tctatatcat tccctggtat gcttggatgg
    11161 gcgaaaatgc taaggctgcc aatggattat tcaatatggg atacaatata tatgcattgt
    11221 ttctactgat ctctacagca ggaatacctg ctgcgattgc aaaacaaact gcacgctata
    11281 attcattgaa tgaatatggc acttcacgac gattatttat ccgtgcatta cagatgatgg
    11341 gcgggctagg cctgttgttt gctttgttca tgtacatcgc ctctccttgg ctagctcatg
    11401 cttctggtgg cggagaagaa ctgattccaa cgatgcgttc tttgagtatc gctgtattgg
    11461 tttttccttg tatgagtgtg atacgagggt attttcaagg caatcaagaa atgatgcctt
    11521 atgcgctgtc gcagattgta gaacaggtgg cacgtatttt ttatatgcta ttggctacat
    11581 tcatcatcat gaaagtcatg gatggtgact atgtgacggc ggtcacgcaa tcaactttcg
    11641 cggcatttat cggtatgcta gcaagtattc ttgtcctttt gtattttttg aagaaacatc
    11701 aagcgtatac atcggcgttt attcactaca gcgaaaacaa aataaatatt gcaacgaagg
    11761 aattgttgtt agataccgtt aaagaagcga ttccgtttat tattgtgggt tcaggggtaa
    11821 cgatcttcaa attagtcgat cagttcactt ttatgaagat catgtctgca tccaccgagt
    11881 attccaatgc acagttactg gatctttttt cgatttttag tgccaatcca gataagttga
    11941 cgatggtagt tattgcattg gcaacatcga tcgcgtctac tgggttaccg ttgattacag
    12001 aagctgttac gttgaaagat cgagtaggtc tggcaaaatt gacaagcgga aatttacagt
    12061 tgttttcatt ttttatgttt ccggcgacat ttggtgttat gttacttgcc tatccgctaa
    12121 atacattatt ttatacaccg gattctctag gaagtaatgt actgatccaa gcaagttttg
    12181 taggactctt cctaggacta tatatgctag tttccaacat gctgcaagga atttttgaaa
    12241 acaaggcggc aattaattat ttggttattg ggtttctagt gaaactcctt cttcaatacc
    12301 cagccattcg tttatttgaa gtatacggcc ctttattggc gacaatgatc ggatttgctg
    12361 tgtcttgtac cttgatctta aagagaatcc atgaagtagc ccgctttaaa ccaagttttg
    12421 tctggcgcag gacattgctg atctttattc ttaccttgat tatgcttttg gcttcgtgga
    12481 tcacaaagat gatttttggt gcttttttaa acgaagatag caaattccag tctctattgt
    12541 tgattcttgt agtagcaggt ataggggctt tagtgtatgg ttatttagct ttgaaaatcc
    12601 gcttggcaga aagactacta ggaccaggaa tagcgcgttt acgacataaa ttaaaaataa
    12661 aataagtgaa tcagaagagc tagattgatg ctcttttgaa atgggggaaa cgcatgcgtt
    12721 tagataagtt tttagcagat atgggacttg gcagccgcaa agaagtcaaa ggattgatca
    12781 aaaaaggaac gatcgaagtc aatggacaag tggtgaaaag cgataaagcg caagtaaatg
    12841 aattcgaaga tgcggttact tatttaggag aaatcattac gtatcaaaaa gatttttact
    12901 acattttgca taaacctgca ggagtcattt ctgctactca ggataatcat gatcaaaccg
    12961 taatcgattt attggaagac caggattatc gggaagatct ttttccagtc ggccgattag
    13021 ataaagacac agagggcctc ttgattttaa cgaatgacgg ggtgtttgcc catcagttac
    13081 tatcacctaa aaaacatgtg gagaaagaat atctagctga ggttagcggt ataatgacag
    13141 aagaagatat ccgtttattt gcagaaggac tgattattga tggcgatgag cagacattgc
    13201 cggcaaaact ccaaattgat caagtagatc aagagacaga aacttcaatt atccgtctga
    13261 ttttgcatga aggaaagttt caccaagtta agcgaatggt caaagcagta ggaaaagaag
    13321 tcttatattt aaaaagaatc cgtatgggaa aattttggct gcctaaagat ttgaataaag
    13381 ggcaatatcg acccatgacc aaagaagagc tagaacaagt gagagaaaaa taattgaatg
    13441 gatagctaaa agaataagca gagtagttgt agcgccatct gaaaagcaga cttgtccgtc
    13501 tgactttcgg atggcttttt tgtgaaattt cggcttattt ccgtagaagc tacttctgga
    13561 acaccgtttg ttcggaataa gggactgtga caagactttt gttacagtcc ccattgttcc
    13621 ttgtgactca gagttagata gtaaggacac gatctcccta atactcaggc caaaacgctg
    13681 aattcacaac ctctgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa
    13741 cggcaaaata tgaggaacta ttttcccgtt tttttctcta atactcaggc caaaacgctg
    13801 atttcacaac ctctcctata ctttgtttca ttttccctat actttgttta tttattctca
    13861 ttattgaaag cgctataatt acgttgcgaa tgaatcgaaa ggggttttta gatgaaaaaa
    13921 cgaaaggggt tcaatcattt ttttatgaat ctatggcgtt ataaagcact tgtcctgatg
    13981 gctgtaccgg gtatgctttg gatgattttt ttcttttaca tacctgtaat ggcgaatgtc
    14041 gtagcattca aaaattttca tatttcagca gatgggttct tggctagctt gagggaaagt
    14101 ccttgggtgg gttgggaaaa tttcaaattt ttattttctt cggatcaagc atttctcatc
    14161 actaaaaata caattttata taatgtagca tttatccttt tgaatctgct gatctcagtc
    14221 gtatttgcaa tcattatgag cgagttgaga aataaacgac tcgttaaagt ttatcaaacg
    14281 atgtccttac taccttattt tctatcttgg gtcattatca gttactttgt ttacgctttt
    14341 ttaagtcctg acaaaggaat attcaatcag tggatcactg gacatggagg agaagctgtc
    14401 aattggtata atgaaccgaa atattggccg tttatcttgg tgttcattgg tacatggaaa
    14461 gggattggtt ataacagcat tatttacttt gcatctgtta tgggaatcga tccgacttat
    14521 tatgaagctg ctatggttga tggagctagc aaatggcagc agatcaaaca tgtgacgata
    14581 ccacaactta ttccactaat gacgatttta acgattttag ccgtaggaaa cattttccga
    14641 gctgattttg gcctgtttta taacattcct aggaattccg gtgcattata tgatgtgact
    14701 caagttttgg atacgtatat ctacaatggc ctgacatcca caggagattt tggtatgacg
    14761 gctgctgcag gactttacca atcagttgtc ggctttgtct tgttgatgat cacaaatacg
    14821 atcgcccgac gttttgatag cgattctgca ttattctaag gaggaaaaaa atgaaaaaaa
    14881 agaaagtgga gagagtccaa gtccgctctt tcaataagac ggctaatttt ttctctaata
    14941 taatgattgc actctttgct atttcctgta ttttgccatt tatatttgtt atcgccatct
    15001 cttttaccga tgagtccata ttgtcgactc acgggtacag tttttggcca gaaaagttca
    15061 gtatatttgg ttatacgtac ttgttcgacc agatgcagga taaaattttc caagcgcttt
    15121 tcgtcactgt acttgttact gttttaggta cactgatcaa tagcacggct acttctttat
    15181 atgcttatgc gatttcacgg tcaaactttc cattccgtcg tttttttacc gttttttgtt
    15241 tgattacgat gttgttctca ccagggatgg tggccaatta tcttgttatg acaaatcttc
    15301 ttcagttaaa agatacgatt tgggcattga tcttgccaat ggctgtcagt cccttcaata
    15361 ttattgtgat gcgcacattc tttaaacgtt cggtctctga ttcgattatt gaatcggcac
    15421 ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat cgttttaccg ttagctgttc
    15481 caggtattgc gacaatcagt ttatttgccg cattgggtta ctggaatgac tggttcaatg
    15541 ctttactata catccaagat gataagctag ttccactaca gtatcttttg atgaaaatcc
    15601 aaagtaatat tcaatatctg acacaaaatt caggagcagg aagccaattg acaggcggat
    15661 tagcctcagt tccaggagaa tctgccagaa tggcgatcgt agtcatctcg acgttgccta
    15721 ttgcgatcag ctatccattt ttccagaagc attttgtcaa aggtctaaca atcggcggag
    15781 taaaagaata aagaaacaaa aatataaatc ataaaattag gaggaagaaa aatgaagtta
    15841 tggaagaaac tagcctttac aggtgtttca gcgttgttgt tgggaacatt agcagcatgc
    15901 ggaggttctg gttctaaaga ccaggcagaa gctagcaatt cggacacttt gcagatgtat
    15961 caaatcggag ataaaccaga taatttcgat caattgatgg aagtagcaaa caaacgaatc
    16021 gaaaaagaga tcggtgtaaa agtcaatatc aattacattg gctggggcga ttacgaaaag
    16081 aaaatgaacg tcatcatttc ttccggggaa aattatgata tcgcctttgc gaacaactat
    16141 gtttccaatg cccaaaaagg agcctttgct gatttgactg aattagcgcc taagtacgct
    16201 gaaaaagctt ataatgattt agatgaagca tatattaaag gaaatctggt caatggaaaa
    16261 ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac aagtacttac ttttaacaaa
    16321 gctttactag ataaatacga tctttcaatt gatggaatcc aatcttacgc tgatgcagaa
    16381 aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg cagcttttgc cattggacaa
    16441 ggcttcaaag tccaagggaa ctttgattat cctttaggga atacgttacc atttgctatt
    16501 gatctggatg gagatgattc gaaaatcatc aaccaatacg ataatgaaac gtatatcgga
    16561 ctgttacgta caatgcatag ctggtacaaa caaggactca ttccaagtga cgctgcaacg
    16621 agcaataagg attatccact agaaggaaat acttggctaa tgcgtggaga aacacaagga
    16681 ccgtatgatt atggcgatac aattttgaca aatgcagcaa aacaagaact tgtctcaaaa
    16741 gcaattactg ttccactaaa atctacagct caagcacaaa tggcaaattt tgtcgtatca
    16801 aatacttcaa aaaataaaga aaaatcagta gaacttctag ggctgcttaa cagtgatcca
    16861 gaattattga atgggcttgt ttgggggatc gagggagaag catgggaaaa agtcgatggt
    16921 tcagatgata aaatcaaatt gctagatggt tatcagccaa atacacatat gtctgcttgg
    16981 aatacaggaa acaacaagat tctatataca caagagtcta ttacggatga catgattgcc
    17041 aaacgtgatc aatcgattgc tgatgcagaa acttcaccga ttttaggatt cagcttcaac
    17101 actgacagtg tcaaaacaga attaagtaat atttctaatg tcatgaatca atatttagat
    17161 ggattgaata cagggactgt tgatccagat gaaacattac cgaaattgaa agacgctttg
    17221 gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa aacaatatga tgagttccga
    17281 caagaataat aatgaataaa gacaaagggg ttgtgacaaa agacgtgtca caacctcttg
    17341 ttccgaataa acggtgttca aaagccagtt tctcggtatt aagctaaata tatgtaaaaa
    17401 tgtggaaagc catattcaca aaatttttct tactactcga aactaagcgg ctatttcaca
    17461 aactcttggt aaacggtgtt caatcagctg accctcggta ttcgctcaca aaacgacaaa
    17521 atatgaggaa ctatttttcc gtttgtttct ctaataatca ggtcaaggtg ctgattgccc
    17581 aacctttttt tgtataatga gaaagtataa acttaatgtt tggaggagaa gatatgttag
    17641 ggaaggtttt ggaagagtta tttatccgga tctgggtcat tataaaacta accttgtatt
    17701 tctggatata tacatttgcc ggcgggatca tctttggatt aggtgcggca tggaaaacag
    17761 tcaatgaact attttatttg tacggattcg agtataaaga aatcacaata aaaagaggct
    17821 ggaatatata taaaagaaat tttttaagag gcaatctgct atttagttta tttttatctg
    17881 gtactgcttt attgagttac aatttatatt tatctgttca aatcaaaggg ctcatttttt
    17941 tagctattga ttttatttta ctttttgcct tgctctgcct atttgctact tatcaataca
    18001 gtatgatttt agatagtgaa tatacgatct ctgttcctaa tctgctaaag ctaagtttta
    18061 tttcggtttt ttccagcttt tcgtcttttc taaaaataat tattggtagc ggtataattt
    18121 taggcgtgac ttggcaattc aaaggattga tcctgtttgg tgtcattggt cttttgacgg
    18181 tttggaatgg tacgatgaca acacattggc gtgaagaatt agataaacag ttggaaagtt
    18241 atgaataagt ttcggattct attttcaaaa ggaaccttac taaatcgtct gttgcggaaa
    18301 tacgcggtta tcatgatcgg tgtaacgatg actgctaccg ttatttttag tatccatact
    18361 tgggaacaaa atcaaaaaca ggcagaaaac atgacaagtg atgccgttca aagcacaagc
    18421 agaatgctga atgataaaac aaccttgagt aggatcatca aaaaccagct tgtagggaat
    18481 tccgaaaaga tcgaaaatgt gacgacctac ttaacaaaac ccatagatca gtatctgatg
    18541 tatgtatacg aacagcaaaa cagtaccgat gaattagttt ctttcccaaa tcaaatcaaa
    18601 gatctctacg caaattatga agaactaagt gcgatatata tcgtgttgaa tcaactaccg
    18661 gagtattatg aatcttctag agaaaacaaa ggtggggaaa taaaaaaggg gacaccaaaa
    18721 ttactagacg ccttttatat caagttgcct atcacacaag gaggagctga gtcaggaagt
    18781 atttttattg gttttcaaaa gaaagaacta gatatgattt tagaaaatct aacgtcattc
    18841 aatggactct ctttatacat gatttctgga acgacaaatc gactatacac attccatgat
    18901 aataaaatag ctaaagaaac gtttgaaaga caagaaatga tcatcacaga gagtctgaag
    18961 aaaaattcac aattgccgat tgacgatctg gaaaaaaaca attttgttca acaccaagaa
    19021 ctgtctgatg attatcgtat attagctgta ctagataaaa aaagtgtaaa aatcgaaacg
    19081 ggaaaaaatc ttgccccgct gatcattggt atcattattt tagacaatat actgcttatc
    19141 tttctttata aaacattcaa acgatattca cagcaagtat ctatcgtcat gcaggccatg
    19201 gatcaaacag cagcagggaa tttagagacg cggatcgaca taacaaacac ggaatatgaa
    19261 ctgaaggaac tttcaatcgg tatcaacgaa atgttggata gcatcaatca gtttgtagaa
    19321 gacatctaca aattagaaat caaacagcaa gacgcacata tgcgggcgct tcaagcacag
    19381 atcaatccac actttttata taatacatta gaatatatcc gaatgtatgc aatcagcgaa
    19441 ggaagtgaag aattagcaga tgttgtgtat gcgttttccg ctttgcttcg aaacaatacg
    19501 aatcaagaga aaacgatcac tttaaaagaa gaactggact tttgcgaaaa atatgtctat
    19561 ttgtatcaaa tgcgttatcc taatcgagta gcctatcatt ttatgatcga tcctgatttg
    19621 gaaaagatcg aagtacctaa atttgtgatc cagccattag ttgaaaatta ttttaaacac
    19681 ggtattgatt ttactcgatt cgataatgcg ctaagtgtca aagtcttgca ggaaggcaaa
    19741 cgggtaagga tcatcatcaa agataatgga aaagggatga ctgaaaaacg gctaaaacag
    19801 attgaagaaa aactgtctca tcctaaagtg gaacttcacg gttctatcgg tctgcaaaac
    19861 gtgaatgaac gtttacgtgc cagcttcggc agcagttatt atatgtcgct tgagaataat
    19921 gagacaggtg gactgaccgt ttctattaca ttcaaggagg gatgagcgtg tatcaagtgt
    19981 tattggtaga tgatgaatac atgattttga atgggttaaa aaagataatt gattggcaaa
    20041 gtttaggctt tcaaatagta gcaacagcag agaatgccaa agaaggacta gctgtacttg
    20101 aacagcgaca gattgattta gtagtaacgg atgtgacaat gccagaaata aatggactgg
    20161 aatttattga agcggctcaa aaagaacgtc ataattttga atttatgatt ttatcaggat
    20221 accaagaatt cgattatttg aaaggaggta tgcaattagg cgccgtgaat tatttgatga
    20281 aacccgtgaa taagttcgaa ttggttgaaa gcctcaagaa aatcaaaaca cggttagatc
    20341 agcaaaatga acaaaaaaat caacaagaaa tctatcaaga actgttattc agtcaatggt
    20401 tgaatgaaga attggatgaa ataagtgaag aagaaataat aggacaaata agtgagaagc
    20461 agcataggat agtattgatt caactggcaa gaactcatga tactcttatc aatagatggt
    20521 tgcaagaacg gcaacaaatc ttttactatc aacgaaatta cggagaccgt gtattgatca
    20581 cgttgttgtt ggaggcagat gaagttgatg gcttctgttg ttttgttcaa gaaaactttc
    20641 ctgatcagga atggctgatc agtatcgggg aagagacaga ttctttggac aaaatccctg
    20701 aaacattcca acaagcgaaa gacaatctac agttgcatca attttatgga gataaagacg
    20761 agcatgtttt ttacgcagta caagcagaaa caaaagatca aaccatcgat ttttcaagtt
    20821 tcagacgcgt gctgcaaaat aaacaattgg aagtcgctca aaaaatgatc actgattttt
    20881 ttgaacagtt tcagttagca gccatgatgc cggaagatat acgatattcc agttttttac
    20941 tgtttatgga gattcaacgg gaattgattg atctggaaga tgaggaatat ttacaaggaa
    21001 tcgaaaaaat ccatcaggca aaaacggtgc aagaacttca gcagctactt ctgtcattca
    21061 tcaaaaagca tcagagacag aaaaagtaca gtgacaatgt ggaaaaagta atcgtaatcc
    21121 tgcaccagca ttatcaagaa ccacttacac tcaaagaagt gtcagaaaac ctgcatttaa
    21181 atgtcatgta tctaggacag ctgttcaaaa aagaaacgaa aaaaagtttt tcagcttatt
    21241 tgaaccattt acgtatggaa aaagcgaaac aactgctttt gcacagtaat caaaacatca
    21301 atgagatagc aagtgagatt ggctacaaca acaccacgta tttctctaaa ttattcaaga
    21361 aaatcgtcgg cagatcccct aaagagtacc gagaaaacag tggagaatca taaaaaaaaa
    21421 agccaatgta atgaaataac ttataaaaaa ttgtctagaa atctaatttt gggctgttct
    21481 tattacttga ggcaaggcag ctttttcatg acctctcgat aaacggagtt caaaagccag
    21541 tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt
    21601 cttaatactc gaaactaaac agctttttca cgacctctcc actaaatggt gttcaatcag
    21661 ctgaccctcg gtattagctc aaaaaatggc aaaatatgag gaactatttt gccatttgtc
    21721 tctctaatac tcaggtcaga acgctgattt cacaacctct caataaacgg tgttcaaaag
    21781 cttgcatctg cggtaataag accaaccaaa cgaaaaatat gaggatctat ttttgcttgg
    21841 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttgttaat tatagtttga
    21901 taaggaaaaa ggcaaatctt ttgcttgata tttcttaggg tgagggatga tccctaattg
    21961 gtaagagatc gtgccgcctt tcagcaaatc ttgatgggta aaatataatt tcgtatattc
    22021 tctgtccatc cgtttgattt tatggataaa ttgttgctgc ggccgattag gcgttgcttc
    22081 aatcgtcaat tcctttccat ttgacaaaag caaaacagcc ttgtcaatta atggcatacc
    22141 gataacatat tcaccggttc caggcgtgac aggataaaat cccagactat tgaagatata
    22201 ccaagcggcc atacttccat tgtcctcatc tccagggtaa cccattggag aatcgtcgaa
    22261 ggtctgagtc atcaactgtt taagcagtgg ctgtgccatt tcaggttgtc ctatataact
    22321 gaacaagtaa ggatagtgaa aacttggctg gtttgaaata gctaattgtc caaattctat
    22381 cgcggccatc tcactcattt cgtggatctc aaaaccatat cctcctgggt caaacgttgg
    22441 gggctgattg cacagctcaa tgagtttttc tttatatctc tcactgccac catattttcc
    22501 gatcagtcct gaaaaatcgt gataaacagc aaagctgctt tgccaagcac ttccttctgc
    22561 ataatcttgt ccccaacgat tagctgaaaa tggttctctg aacttgccat ctttatcttt
    22621 tgaacgcata aatcctgact catagtcaaa gatattttga tagttcaatg attgctgctg
    22681 atatttcttc gtcatttcct cttcttctag taccttggca acttggctaa tacaaaaatc
    22741 gctataagca tagtctaatg tgtgattgac tgattcgtgg tagtgattag gaacatagcc
    22801 atatgtcaag taatcttctg ttccttggcg tccataattt ggatgtttgc tttgaaccgt
    22861 agcacctttt tccattgccg ttagaaattc aggcatcaag tctcgtcgga tatcttttac
    22921 agcagcatct gcaatgaccg catcgatcaa tgtcccaggc ataagtccac gttcatcagg
    22981 agacagccat tttggcaaat agccactttc tctatagctg ttcaaaaaac cttccagcat
    23041 ttcttcgtat ttttctacag caatcaaaga ataaagagga taaaccgttt tatacgtatc
    23101 ccaaaaccca ttatttgtat agagaacacc aggttttata gatcgactag tcgtatcata
    23161 gtggattttt tgcccactag gatcaatttc ataaaatgtt tgtggaaata aaaacacccg
    23221 atataaatta tgataaaacg tccgcaattg ttgttgatca tggtgtgtga gctgtatacg
    23281 atcaaaatag gactgccatt gttcttgact gtgagcaagg taagtctctt tgctttggtt
    23341 ttgttctagt tccaggttat aagctgcttg ttccgagctg ataaaagatg tcccaaatcg
    23401 gatagtttgt ttttttgtct gttcaaaata caacgggatc gagtcgctat ttttgttgga
    23461 aacagctaac tcatcaattg ttaaaggata ttcaaatctc aatataaaat aaaaagaaaa
    23521 atcaggatct tctgaaccag caaaatgtgt gatttttcct tccactgtgt aaggatctat
    23581 aacaaagagc tgatggcgtc ccggaaagga taaaagtaaa ccgctgtcat ttttgctgta
    23641 atcaatagat aagattccgc catacatgct aggtatcaat gtagaacaga tgccataacg
    23701 taattgacag atcgacagat gagtcgggca aaatatactt tcttctgggc ggtaagaact
    23761 ttgagcatgg aaaagggtgt tctcctgtag ttttccattg atcggcgtaa acagcatatg
    23821 actgaaatca cccatccaag gacttggttg atgggtaaga cggtagcctt gaaaaacgcg
    23881 gtcatcagga tgaaaccacc agctgccgtt ttgatccgta gtttgcggtg caaaataatt
    23941 catcccaaaa ggaacgccag tataaggctg gcagttccca ttagagaaat ttggctgatt
    24001 cgctgttgca tgtctagtat ctatttgacc gatattcatt ttatttctcc tttgttgatt
    24061 gtcttttgat gaatgaaact ggaaatttac tatcggatgg tcgatctttt gtctctaacc
    24121 aagtgaccaa agcatctccc gcaagcttac ctaactgttc aaaatcttgt gcgatggttg
    24181 tcagtggcgg gtcaacaaga gctgctgctt ggatatcatc aaatccgata attgaaaaat
    24241 cttcagggat atgataacca tgctgtctca agagattcat ggtctggata gcgacaacat
    24301 cgttttcaca aacaaaagca gttacttcat acttagtgat ataagaaagc aattcatcta
    24361 atgtcgcgct tttgtcatcc agtgaagtgt ggaaagtcaa accagctttg tccaatgctt
    24421 caaggtaacc aaggtaacgc tgtcgaacgg attgtggatg agtctgcgta ccaaacaaat
    24481 aagcgatttt tgaatgtccc tgttcgatca aataagaggc agcaagcgca ccgccttgga
    24541 aattatcgga aagaacggta ggaaaaggta actcatagat ttttttatct aatatgacga
    24601 cagggaattc tttcaatgaa agctcaaaaa gtaagtcaag atgttgttct gtatcaagag
    24661 cataataaat caatccgtca aattcattga ttagctcttt tgctgtttta ttttgtagaa
    24721 agtcaagcgt cgtcatcatg acatcaatct gtttatcttg catcacaggg ttcaatccct
    24781 tgctaaaatc accaacagac aaatcagcga catatggaag aagaaacaga atccgtcgtg
    24841 tttttgtagg agaagcaaga atctgttccg gagaagcgga tttaacgaaa ctgcctttcc
    24901 cgcgaacgcg ataaataagt cctaattgct ctaattcagt caatgcacgt ttggaagtga
    24961 ttctgcttac tttataggta cgtgaaagtt ctttttcagt tggtagttgg ctgtctacag
    25021 gtaattcacc agttaaaata gccgtttgca aatcattaaa tattgtctgg tacaatggtt
    25081 gagtcatata aacacctctt aatgatatat catattcaaa taataccata atggatgaaa
    25141 aagtaaataa aaagatattt tttgaaagcg tagacataat tggctgtatg aagtataatc
    25201 tttaaagata tatcaaatag aaagaaggtt ttattagtgg tttataaaga tataccaaaa
    25261 tcggtgcaga aatttatgga agatatcaca caaaaatgtg gggatatcca tgcggactgg
    25321 gcaaagaatt ttacagcagc atttgccaat actctgttga caacagtcaa aaagcaaaat
    25381 gacggaacca cctttttatt gacgggagac attccagcga tgtggttaag agactctact
    25441 gcacaagttc gtccttattt agtgattgca aaagaagatg aggaattagc tgacatgatc
    25501 agcgggttag tcaaaaagca atttttttat atcaatatcg atccatatgc gaatgccttc
    25561 aacgaagaag ctaacggtgc tggccaccaa acagatcata caaaaatgaa cgattggatc
    25621 tgggaaagaa aatatgaaat tgattcttta tgctacccaa tacagttggc ctatcttctt
    25681 tacaaaaata caggaaaaac tgatcaattc aatcaatctt ttattgaagg tatcaaaaaa
    25741 atcttggacg tgttcaaaac agagcaagat catgaacact ctccttatac atttgtacga
    25801 gacacgacaa gaaaagaaga tacgctgatc aatgacgggc gaggtgcaaa aacagctttt
    25861 acaggaatga catggtctgg tttccgtcct agcgatgatg cttgccgtta tggatatctg
    25921 gtaccatcca atatgtttgc ggtggtcgtg ttaggttatg tggaagaaat ttttcaaacg
    25981 atactcgatg ataaagaaat tgtacagcaa acacagaaat tgaagaaaga gatccaagag
    26041 gggattcaga atcacggacg aacggttaat caaaaagggc aaacgattta tgcatatgaa
    26101 gtagatggaa tgggaaacgc cacagtcatg gatgacagta atgtgcccaa tctgatcgcc
    26161 gcaccttact tgggatattg ttctgaacaa gaggaagatt atctgcaaac aagaagaaca
    26221 ttgttgagta aagaaaatcc atatttctat gaaggaaagt acgcaaaagg aattggatcc
    26281 tcccatacgc cagaaaaata cgtttggccg attgcattag ctatggaagg aatgacaacg
    26341 tcagacaaat ctgaaaaaga gcggatattg aatttacttg tagcaactga cgcaggaaca
    26401 caccttatgc acgaaggatt cgatgtgaac aatcctgaaa attatacgag agaatggttt
    26461 tcttgggcaa atatgatgtt ttgcgaatta gtcatggatt actttgatat tagagtagaa
    26521 aagtaggagg tatcgtaaat gaaaaagaaa gtatatatca tttctcatag ccactgggat
    26581 cgtgaatggt acatggctta cgaacagcat cacatgcgac tgattgaatt gatagacgat
    26641 ctactagagc tatttgaagt tgatccgtcc ttcaacagtt ttcatttgga tgggcaaacg
    26701 attattttag atgactatct tcaagtaagg ccagaaaaaa gacaggcaat ccaacaagca
    26761 atcaatgaag gaaaattgag aattggacca ttttatatct tgcaagatga ttttcttatc
    26821 agctcggaat cgaacgtgag aaatatgctg attggcatgg aagaaagccg taaatgggga
    26881 acccctgtca tgttaggcta ttttcccgat acatttggaa atatgggaca gacgcctcaa
    26941 ttaatgaaac aagctggtat ttcagcagca gcgtttggcc gtggtgtaaa accaattggc
    27001 tttgataatc aagttttgga agcagaaaac tattcttccc aatactcaga gatgtggtgg
    27061 aaaggccctg atcaaacagc aatctttggt ctgctatttg ccaattggta tagcaacggg
    27121 aatgagattc cagtagaaaa agaagcagct cttgcctttt ggaagcaaaa attagcggat
    27181 gcagaacaat atgcctctac gaaccactta ttgatgatga atggtgttga tcatcaaccg
    27241 gtacaaaaag atatatcaaa agcaatccat ctcgcaaatg aactgtttcc agattacgag
    27301 tttattcatt ctaattttac tgactatctg gaagcagttc aaaaagatgt gccagaagac
    27361 ttaggctcag tagaaggaga attgacaagt caagaaacgg atggctggta tactttagcc
    27421 aatacagcat ctgctcgggt ttacttaaaa caatggaata ccaaggtcca gcgacagcta
    27481 gaaaatatta cagaaccact tgcgacgatg gcttatgaag ttagtggaaa ctatccccat
    27541 gatcaattag attatgcttg gaagacatta atgcaaaatc atcctcacga cagtatttgc
    27601 ggttgttcag tcgattctgt ccatcgggaa atgatacctc gttttgaaaa agcagatgaa
    27661 gttggaaagt atcttgcaca agattcctta gaacagctga ccgcagcaat cgatacgacc
    27721 ggattcccaa aagacagctt tccatttgtc attgtaaata ctgcaggaat ggataaaact
    27781 ggtgaagcag aaataaccat cgaattagaa agaaaaagat tcgctgaagg aatacctgaa
    27841 caactatatc aagagttaga aaatttgcct aaacgtaagt atcatgtaga aacaaaaagt
    27901 ggggccacta taccagctat tttatctgaa gaaactgtcc aatttggtta tgatttgccg
    27961 aaagaccgtt tccgtgtgcc atatatggca agaatgatca aagtgacttt accattagaa
    28021 aacatgccag cattttcttg ggaaacattc gcgcttgtag aaggggaagc gaaagcagaa
    28081 gaaaaggaaa caatgattca tcagagtgga cgaatcatcg aaaacggccc tctacatttg
    28141 acgattgaaa aaaatggtac cattacaatg gaagatcgca aaaacaaacg aaagttgaat
    28201 aatctgcata tttttgaaga tatcggcgat ataggaaatg agtatatctt taaacaacct
    28261 ttctgtgatc agccaatcct atccagtaac aaagaaaact ctgaagtaaa agtattagta
    28321 gatacaccgg aaatagcaaa aataagtatt ctacaggaaa tggaaatccc tgtttctgca
    28381 gacgagcgtt tagaaaaaga acagcagatg gttgtggaat ttcgttatcg aaaagcagaa
    28441 cgatccaaag aaaaacgaat ccttcaaata aaaacaatca tgacaatcag aaaagatagt
    28501 aaaaaaatcg actttgaaac gactctcgac aatcagatga aagatcatcg cttacgtgtc
    28561 ttgtttccta caaagttaca tgtggaacat cacgaagcag atagtatttt tgaagtggtg
    28621 aagcgcccaa accatgtgtc taaaagttgg gaaaacccca cgaatccgca acatcagcaa
    28681 gcatttgtca atattcatga tgaagagtac ggtgtaacag tcggaaactt tggtttgaat
    28741 gaatatgaag taacagaaga cggacaaatc gcagtgacgc tacttcgcag tgtgggagaa
    28801 ctaggcgatt ggggatattt tccaacacca gaagcacagt gtctaggtga gcatcggttc
    28861 aattacagca ttgaactaca tggaccagaa gaaaaattct ctacttattt gcatgcatac
    28921 gcagcacaga ttcctttttc cactcaacaa atcaaacatc atgaaggaac attgatttca
    28981 aaacaacaat atttaacgat aaaaagtgaa acgtttgcaa ttacagcttt gaaacgcagc
    29041 aagtttagtg ataaagtagt agtaagaggt ttcaatatgt ctagtcattt agaaaaattg
    29101 gaaataacta aagacaatgg aaaaactgtc atattgaatc tgcttgaaga accaacgaag
    29161 caagcggttg tcccaataat ccaaccttat gagattcgga caatcggatt cgaggaggaa
    29221 aacgaatgta tggcggaata gaagcaggcg gaacaaaatt tgtgtgtgcg gtttcagatg
    29281 gtggagaaat tttagaaaaa ttaagtatcc ctaccacaac accagaagaa acattggatc
    29341 aggtatttga attttttgat cgttatgaat tgaagggaat cgggattggc tcattcggcc
    29401 cgattggtat cgacaaaaca aaagataatt acggatacgt tttggcaact cccaaaaaag
    29461 ggtgggcaca attcgatttt cttggcacaa tcaaggaacg ctacaatatt cctgctgctt
    29521 ggacaacgga tgtcaatgct gctgcatatg gggaattgaa gcaaggagca gcacaaggaa
    29581 aaaatagttg tatttacctt accgtgggaa caggaatcgg tgcaggtgtc gtgatcaatg
    29641 aagaaatttt ttcagggatc gcacatcctg aaatgggaca tatttgggtg aaacgccatc
    29701 cagatgacca ttatgaaggt acttgtcctt atcataaaga ttgtttagaa ggattagcag
    29761 caggaccgtc tattgaggca cgcacaggga tcaaagggca agatctgccg caagatcatc
    29821 ctgtttgggc tattcaggct tattatattg ctcaagcact gatcaactat acgttgacgt
    29881 tagcacctga aaagattatt ttaggcggtg gggtaatgaa tcaggatcat ctccttcaaa
    29941 aaatccgtca gcaatttgtt gaattgatgg gtggttatat ggaaacacct caagtaagtg
    30001 aatatatcgt tcgttgggga ctgccgaatg aaagtgggat tatcggtagt ttgctgttag
    30061 cagaacacga acatcaaaag taagcagaca aattacacaa aaagaacaga gtcttatcat
    30121 tatctgctga taagactctg ttctttttta tttctattcc cataatccag gtaaagcttt
    30181 accgactatt ttttcttgag taaatggatg gatcatgtgc agttctaatg catggagcat
    30241 cagtcgttca gctgttctat tttggtagag agggtctcca acgatcggat gtcccttatg
    30301 agctaaatga actctgatct ggtgtgtacg tccggtttcc aaaacacaat agacttgtgt
    30361 ttgttcagaa agtactttgt tgacagtgac atgtgtcaag gcgctctgac catttttatc
    30421 atctacgacc ctttttctcc gatcatggcg atctcgtcca attttattgg tgatggtcat
    30481 atctttggca atttctcccc atacgatggc ttgatatctc cgatagatca ttttttgttc
    30541 caacattctt ccaagtatcg gcaagacaaa tggatttttt gcaaataaaa cggcaccgct
    30601 tgtttctttg tccaagcggt ggacaacgta aggtcgttgc tgtttagtgt cgagataagc
    30661 agctaaatga ttgagtaagg tactgttttc tgtcggttca tttggatgag tcttcattcc
    30721 tgcaggctta tccactataa tcaaatgctc atcttcatat aaaacacgga tgtgcttcgc
    30781 atctcctaat tgaacttctt gataattata atctgtttct tcgaataaca atgtgatctg
    30841 gtcattgccc tttacttcat gatggaacaa agcaggttgt tgattgatcc aaacgttttt
    30901 acgtatacgc aaaaagtgtc gaacctttct aggaactaac cattcctttt caagcaagtc
    30961 gcgaattgtt gtttcaggat ggttcatagg taaagtgatc gtgattttca caagtgtact
    31021 cctttcagtg caattgcaga ctaattgtac catttttaaa acatatttaa gaaaaaactt
    31081 acatttttct gttcattctt atgttactct acttgcacta acgtaaaaaa acatattgaa
    31141 ctacaactta agcaggaaga aaacactctt tcactatggt aaaataagac gattgaatca
    31201 aatagacagg agaagaagca aatggatttt caaagcattt tagaaaagat aaaagctgct
    31261 ttgatcagtt tttggcgttg gatcagacca tatctcggac aattccatcg atggcgaaag
    31321 cgcatctgga aaaaatatca tgtgaacaaa ttgattttgc tgattggttt gatctgtgtc
    31381 ttagtgacaa gtatctacct ctttatttta gcaaaacaag caaatgtcga aacgttaaaa
    31441 tcaggattaa gccaatcgac agtcatttat gataagaaaa acgaagaagc tggcacactg
    31501 tacgcccaaa aaggaacata tgtagaatta aacgcgattt ctccctatat ccaagatgca
    31561 gtaatctcga cagaagatag gaatttttac gagcatcacg ggtttgatat caaagggatt
    31621 gcccgtgcag cagtccgaat ggtcttgtct ggcggaacat cagggggcgg aggaagtacg
    31681 atcactcagc agcttgccaa aaatgcgtat ctgacattag accaaacgtt tgatcgaaaa
    31741 gcgaaggaat tatttttggc aatcgagatc gaaaagaagt acagcaaaga agaaatcttg
    31801 accatgtatc ttaacaatgc ctattttggc aacggggtat ggggtgtcca agacgcttcc
    31861 agaaagtatt ttggagtaga tgcaacgaat gttacgctgt ctgaggctgc tactttggca
    31921 ggaatgctga aaggtcctgg tatttataat ccgatcgatc acccaaaaaa tgcgaatgat
    31981 cggaaaaaca cagtactaag tgtaatggaa gaaaacggaa aaatcacaaa agaacaagaa
    32041 caagcagaaa gccagacaga tatcagcgcg tatcttaatg acacatacga taattcagat
    32101 tctggttatc gttatcctta ttattttgat gcagtgatcg atgaagcagt taacgagtat
    32161 ggtcttgatg aagaagatgt catgaataaa ggctataaaa tctatacagc tttagatcag
    32221 gattatcaaa agcaattaga agctacttat caaaacagtg cattattccc agcaaatgct
    32281 tcagatggtg ccatggtcca atctgcttca gtagctttag atccgaatac aggtggtgta
    32341 caagcactag taggacgaag aggagatcat gtctttagag gattcagctt tgctacacag
    32401 atgcgtcgtt caccaggatc aacgatcaaa ccattaagcg tatatacacc agcactggaa
    32461 gctggataca agccttccag tatcttgaaa gacgaaccgc aaagttatta cgatgcgcat
    32521 aattttgacg ggacatacca aggagaagta ccgatgtatc aagctgttgc acaaagtttg
    32581 aatttgcctg ctgtatggct tttgcatgag atcggtttac aaaaaggata tgataaagca
    32641 gaagaattcg gcctgccttt agcaaaatct gataagtatt atggcttagc ccttggtgga
    32701 ctggaaaaag gagtatctcc attgatcatg gcgggtgctt acagtgcatt tgccaatgat
    32761 ggtcagatgt atacgccgca tcttatcacg aaaatcgttg attcgactgg agcggtgatc
    32821 gtcgataaca caaacaaaaa accaaaacag gtcatatcaa aggaaacagc cgatgaaatg
    32881 accagcatgc ttcttggtac attttcaaat ggaacagcgg ctgctgcgaa tcctgcaggt
    32941 tatacgatag cagggaaaac aggaaccaca gaaactaatt tcgatgccac aaaagcaaat
    33001 gaccaatgga tgatcggtta cacgccagac gtagtgattt cgacatggat gggttacgaa
    33061 gaatccagca agctgcacta tttggaaggg acaagtggaa atgttgtcgg gaaagtcttc
    33121 aaatcagcgg cagaaggcat ccttccgtat acttcaaaaa caggatttag tgtagcagat
    33181 gcctatgcca caggaggaca agtggtagct gcagatcaag ttggaaacac aggaaattct
    33241 aatggagagt ctggaaattg gcaagataac ttgaatcaat atggtgaaca agcaaaagaa
    33301 gggctgaaaa acttcggcga tatggtgaaa gaaggcgttc agggaattgg tgaagcggct
    33361 aaagacctct ggcggaaata tcaaggagag tagccccttt gaaaacgaat aaccccatgt
    33421 tacaatagag agtaactact aaaagaaaag agggcataac atgtcaaaca tctacgatag
    33481 cgcgaatcaa atcgaacgtg agatccgtga attaccagaa ttcaaagcgt tgaaagaagc
    33541 gtacgagaaa gtacaagcaa acgaagaagc acacaaactg ttcaaagact tccaagcgat
    33601 gcaaatcgaa ttacaagaaa aacaaatgag tggacaagag ttcagcgacg aagatgcaat
    33661 gaaagcacaa gaaatggctg tgaaaatcca atcagaagaa gtaatcaatg acttgatgca
    33721 aaaagagcaa ggcttcagta caatcatcaa tgacttgaac cgtatcatca tgacaccagt
    33781 tcgcgacctt tacagcgaat aaaactattt tatgaaacaa cagagggtgt gaaagagacg
    33841 ttcggccttg agcgatgaca aaattgcttc ttattgccga taggctgtct cttgaacacc
    33901 atttaccaag aggtcgtgag cctaccgttt aactctgagt tacaaggaac aatttcaaaa
    33961 aacagcttct catgtttgat gactattgtg acttgtgacc gaggagttgg caggagaaca
    34021 ccgtttattc agaaatagga gctacgacaa aagtcttgtc atagctccta ttttttgtca
    34081 ttcatttttc aagcggtaga aatagccttt ttgagctaac agtttttcgt aagtacctat
    34141 ttcaacgatt tttccttcat cgatcacaac gattttatct gcctctttga tcgttgaaag
    34201 ccgatgagcc acaataaaag tagtgggtgt ttctgtcagg taattcaggg cttgctggat
    34261 ttttttctct gactggttat ctaaagctga ggttgcttca tctagcaaaa tgatttttgg
    34321 ctgacgtatc aacgctcgtg caatggagat acattgcttt tggccaccag acagcttgtt
    34381 gccatgttcg cctactaagg tatccagacc atcaggaagc tggtcaatca gtgaagaaag
    34441 ttgcgcagct tcgatgactt catctaaacg ttcttttgat acgtttttca agccataagt
    34501 gatgttctct ttgatcgaag cggagaacaa caaagtggtt tgaggaacaa cggctaaata
    34561 atttcgcaca cttcgcatat ccagttcatc caaaggctga tcatctagaa aaatttttcc
    34621 tgaagtcggc tggataaatc caatcaataa gttaaggagt gtagattttc cagagccaga
    34681 tggcccaaca aaagcaattg tttctccagc tttcacctct agagaaaaat gatcgataat
    34741 cggtttgagt gagtccggat aaagaaaaga aacatcctca aaacgaaaat ttccatccaa
    34801 atgttggatt tttttcttac cgtgataagt ttctacatcg ccagcaatca acagttcgcc
    34861 aatagaagtg atagattcca tgccttttga aatcgtagga atcagcgtga tcattccaga
    34921 aacggaattg acgatcgtcg tgaaatacgt ctgatacatc acgacatcgc cgggttggat
    34981 gcgcccttgt aaagctaata tcccagtgaa actcaaacaa atcagctgaa atatctgaaa
    35041 actagcccag ctgactgatc cgaaaacaga ttgcaggata tctaagtgga atcctttttc
    35101 atagatttga gtaaaatgct tatttaagcg agttgtttcg tgtttttcta acccatgagc
    35161 tcgagcaatc ggtgctaatt cgatcacttc gatcactttt gcagaagtct cttccatttc
    35221 ttgcctgaag tctgagttgg actgtctgat ccttttacgg aaaaagagag taacgaatgc
    35281 ggcgagagga acagtcaata agaaaaacat gaaaacaaca ggacttttat aaagcgtgat
    35341 acctaaagct acgaataaat tgacagcgat atttaatccg ctgacaaata cttgagagga
    35401 caacgtttgg atagcttcta catcacggat gatctttgac tggattcgtc cagattgcaa
    35461 ttctttttga taaggaatag atagttcttg gattttcttg ataagggtcg ctcttagtcc
    35521 agcttctact tgacggatcg aagtgctatg gtattttaca tgaaaataat tcgtcaatat
    35581 attttgacca atcagacaag ccatcaaaaa agcgttgaaa taaatggcct gcaactgaga
    35641 agaatctttc gatgaagctg cattgattac attggcaata gcgatcggca ttaaccaaga
    35701 tggaagatgt ttgataataa aaaagagtcc tgacatggca aaacgccaat aatttccctg
    35761 ataaagatgc agcaatgtct gaaaagcatt tgcatgatat ttttgataac ttttctgtat
    35821 tcgttcctcc ataatcttct ccttaacaat cttggtaaat aaactgcaag tgttctattg
    35881 cttcaggaac ataaatctca ggagtagctt caagagtggc gtacattaga ggacgctcat
    35941 atttgatata atgcagtacc gggcggaaat ctaaacagcc cttgccaaca ggtaccatgc
    36001 ggatctttcc attttccatg atatagtctt tgagatgaag cgctgtaata taaccatgta
    36061 aattgtccaa ggcacctcgg atcacttttt cctgatcagc atgattttct ttatgcatca
    36121 aattcgcaca atctaaaatg atttttaaat ttggtgaagc tacttcatcg attaaacgtt
    36181 tagctaactg atagttatag attggatgat tgatccctgc ttcaacagca acagttacgc
    36241 caaaattttc tgcatgagcg actaattcaa tgatagaatt tctggcgatc tgataagctt
    36301 cttcagtaaa attttctttt gtgtaaccga cagagacact accggtttcc gtagcaatca
    36361 tcttggcttg ataggcagag cacagagcaa tttgctgttt gaaagttgcc aatgcttgtg
    36421 cacgaacttc ttgatttttg ctggataggt tgatatagca tcctaaaatg gaaagttcga
    36481 tattgttttt ttggaagtat tcaccaaaat aggcagcact cccaagtgtc atgtcagtta
    36541 atttaagttg ttcaggaaat gctttttgtg gtgcaaattg gatatgggac aagtgaagct
    36601 gtgatacttt ttgaacgatt tcttcacgat ttcgaacatc taaatcgtgg gcacggatgc
    36661 ctaaatttaa tggaaacatc tcaaactcct ttttgtaaat aattagcaag aggcagacgg
    36721 gattctttta tcaattcaac aattattcgg gcaactttat cagcgccttc tggactaaag
    36781 tgggtattgt cttttagtcc ttctgggtaa tttttacaag tatcaggcat aagatgcagg
    36841 tagaattttt ttgtctcttc ttttgagaag gtctggaaca attcttgtga tcttgagaac
    36901 acatctaaaa gaggaatatg gtgtttctca gcaaatgcac gcatcgcttt tggatagtct
    36961 ccaagtatat ctggattcaa tgttccgttt tctaagtaat ctcgtcgcgt cacagatgtc
    37021 agtaagacca tttggacgtt cgcttttttt gcgcattcaa aaaatatctg cagatttttt
    37081 aaatagctac cgaagggaag cgtacctctt tcttcttgga ttttctggtc gttatgtcca
    37141 aattgtacaa ataagaaatc attcggctga aactgtgaag ctagacgggc taaccttcct
    37201 tcctggataa atgatttggt gctgcgtcca ttctttgctt ggttattgat ctggatctcg
    37261 ggtgaaaaat aagcagagat tttttctccc caacctgtct cgggacgctt gttttcatct
    37321 ttttctgccg cagtagaatc accggcaata tggatagtag gcatgttatc tcttctttca
    37381 taaaggttta taggcaatga agcagaacag caagagaaga agacggagca a
//
LOCUS       DAPBCS010000026        37248 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPBCS010000026 DAPBCS010000000
VERSION     DAPBCS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.026"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(144..2297)
                     /locus_tag="RD521_001521"
     CDS             complement(144..2297)
                     /locus_tag="RD521_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDT7771262.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2692..3540)
                     /locus_tag="RD521_001522"
     CDS             complement(2692..3540)
                     /locus_tag="RD521_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7771263.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3764..5704)
                     /gene="thrS"
                     /locus_tag="RD521_001523"
     CDS             complement(3764..5704)
                     /gene="thrS"
                     /locus_tag="RD521_001523"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDT7771264.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6041..6220)
                     /locus_tag="RD521_001524"
     CDS             complement(6041..6220)
                     /locus_tag="RD521_001524"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDT7771265.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6267..8090)
                     /gene="cadA"
                     /locus_tag="RD521_001525"
     CDS             complement(6267..8090)
                     /gene="cadA"
                     /locus_tag="RD521_001525"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDT7771266.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8368..10647
                     /locus_tag="RD521_001526"
     CDS             8368..10647
                     /locus_tag="RD521_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7771267.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10740..11387)
                     /locus_tag="RD521_001527"
     CDS             complement(10740..11387)
                     /locus_tag="RD521_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771268.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11566..12285)
                     /locus_tag="RD521_001528"
     CDS             complement(11566..12285)
                     /locus_tag="RD521_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771269.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12416..13567)
                     /locus_tag="RD521_001529"
     CDS             complement(12416..13567)
                     /locus_tag="RD521_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDT7771270.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13784..14524)
                     /locus_tag="RD521_001530"
     CDS             complement(13784..14524)
                     /locus_tag="RD521_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7771271.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14528..14851)
                     /gene="rsfS"
                     /locus_tag="RD521_001531"
     CDS             complement(14528..14851)
                     /gene="rsfS"
                     /locus_tag="RD521_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDT7771272.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14908..15501)
                     /gene="yqeK"
                     /locus_tag="RD521_001532"
     CDS             complement(14908..15501)
                     /gene="yqeK"
                     /locus_tag="RD521_001532"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDT7771273.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15485..16135)
                     /locus_tag="RD521_001533"
     CDS             complement(15485..16135)
                     /locus_tag="RD521_001533"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDT7771274.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16145..16456)
                     /gene="yhbY"
                     /locus_tag="RD521_001534"
     CDS             complement(16145..16456)
                     /gene="yhbY"
                     /locus_tag="RD521_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDT7771275.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16472..17581)
                     /gene="yqeH"
                     /locus_tag="RD521_001535"
     CDS             complement(16472..17581)
                     /gene="yqeH"
                     /locus_tag="RD521_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDT7771276.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17578..18105)
                     /locus_tag="RD521_001536"
     CDS             complement(17578..18105)
                     /locus_tag="RD521_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDT7771277.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18285..19070)
                     /locus_tag="RD521_001537"
     CDS             complement(18285..19070)
                     /locus_tag="RD521_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDT7771278.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(19063..19932)
                     /locus_tag="RD521_001538"
     CDS             complement(19063..19932)
                     /locus_tag="RD521_001538"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDT7771279.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19933..21312)
                     /locus_tag="RD521_001539"
     CDS             complement(19933..21312)
                     /locus_tag="RD521_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDT7771280.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21320..21739)
                     /gene="fabZ"
                     /locus_tag="RD521_001540"
     CDS             complement(21320..21739)
                     /gene="fabZ"
                     /locus_tag="RD521_001540"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDT7771281.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21764..22240)
                     /gene="accB"
                     /locus_tag="RD521_001541"
     CDS             complement(21764..22240)
                     /gene="accB"
                     /locus_tag="RD521_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDT7771282.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22243..23478)
                     /gene="fabF"
                     /locus_tag="RD521_001542"
     CDS             complement(22243..23478)
                     /gene="fabF"
                     /locus_tag="RD521_001542"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDT7771283.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23497..24234)
                     /gene="fabG"
                     /locus_tag="RD521_001543"
     CDS             complement(23497..24234)
                     /gene="fabG"
                     /locus_tag="RD521_001543"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDT7771284.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24246..25163)
                     /gene="fabD"
                     /locus_tag="RD521_001544"
     CDS             complement(24246..25163)
                     /gene="fabD"
                     /locus_tag="RD521_001544"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDT7771285.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25270..25494)
                     /locus_tag="RD521_001545"
     CDS             complement(25270..25494)
                     /locus_tag="RD521_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDT7771286.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25547..26509)
                     /locus_tag="RD521_001546"
     CDS             complement(25547..26509)
                     /locus_tag="RD521_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDT7771287.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26519..26959)
                     /locus_tag="RD521_001547"
     CDS             complement(26519..26959)
                     /locus_tag="RD521_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7771288.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27265..28230
                     /locus_tag="RD521_001548"
     CDS             27265..28230
                     /locus_tag="RD521_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDT7771289.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28287..28517)
                     /locus_tag="RD521_001549"
     CDS             complement(28287..28517)
                     /locus_tag="RD521_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDT7771290.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28603..29490)
                     /locus_tag="RD521_001550"
     CDS             complement(28603..29490)
                     /locus_tag="RD521_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDT7771291.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29579..30802)
                     /locus_tag="RD521_001551"
     CDS             complement(29579..30802)
                     /locus_tag="RD521_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7771292.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30819..31865)
                     /locus_tag="RD521_001552"
     CDS             complement(30819..31865)
                     /locus_tag="RD521_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7771293.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(32029..33756)
                     /gene="ptsP"
                     /locus_tag="RD521_001553"
     CDS             complement(32029..33756)
                     /gene="ptsP"
                     /locus_tag="RD521_001553"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDT7771294.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRGLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33756..34022)
                     /locus_tag="RD521_001554"
     CDS             complement(33756..34022)
                     /locus_tag="RD521_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDT7771295.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34501..36735
                     /locus_tag="RD521_001555"
     CDS             34501..36735
                     /locus_tag="RD521_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7771296.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36883..37203
                     /locus_tag="RD521_001556"
     CDS             36883..37203
                     /locus_tag="RD521_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDT7771297.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        10482 a         8061 c         6484 g        12221 t
ORIGIN      
        1 gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg tatgaagtca
       61 aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac tcgaaactaa
      121 gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata cgcgtccatt
      181 atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt cggcaagaca
      241 acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt actattgact
      301 gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt tgcggaaagg
      361 tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc atggacaact
      421 tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac cgtattttct
      481 actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc attgccatag
      541 atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt tgaagcatat
      601 tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc ggtaaagtta
      661 cctggagcat accattcttt gccattatcc gcattgaact tcttcatata atcaacagaa
      721 cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc aagaccaaac
      781 tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa actaggtagt
      841 cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat catatacgta
      901 ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct tcgattaaag
      961 acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa aacttgattt
     1021 ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac tacagaccct
     1081 ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt ggaaccttgt
     1141 tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat agccactgca
     1201 taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata attccttttc
     1261 aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa attagaacct
     1321 ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc aagggaaact
     1381 tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa ataactttgt
     1441 cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc atctgctgga
     1501 agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact tgctgcatta
     1561 tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc gcgttctgcc
     1621 acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt gtttaattca
     1681 tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc gtttaaatta
     1741 atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt ccctaattgc
     1801 ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc agccagccaa
     1861 aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat cagcgagttc
     1921 agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct cgtatatgtg
     1981 atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta gatcattccg
     2041 cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc ctccatctct
     2101 tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa taacgtaaat
     2161 ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa cgttttgttc
     2221 ttaggttctt gtgcaccttt tcttattttt tctaaccaag aagtatttaa tatttttttc
     2281 ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt cattgtaccc
     2341 aaaaacttct taataaacaa gaagtattta atattttttt cataaaatct ttcatcattt
     2401 cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc ttaataaaaa
     2461 agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat caactttttg
     2521 tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa aaataaactt
     2581 atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat ttatttgtgg
     2641 aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt tttatttcga
     2701 atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc taagcttttc
     2761 gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga
     2821 attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc
     2881 ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat
     2941 gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag
     3001 ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt
     3061 ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa
     3121 agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac
     3181 tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt
     3241 gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc
     3301 ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg
     3361 aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc
     3421 taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa
     3481 ctttttttct gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat
     3541 aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca
     3601 cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat
     3661 caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc
     3721 ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt
     3781 aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt
     3841 ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa
     3901 ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat
     3961 tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag
     4021 cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc
     4081 atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag
     4141 aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat
     4201 atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt
     4261 ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc
     4321 cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg
     4381 ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc
     4441 tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa
     4501 gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca
     4561 acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca
     4621 tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt
     4681 agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca
     4741 tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca
     4801 actcaacatc tcccattatt ggtgtatata catgttggta acctagacta acttctttgt
     4861 ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca
     4921 aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat
     4981 ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa
     5041 aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag
     5101 cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc
     5161 cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca
     5221 attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt
     5281 ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt
     5341 cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa
     5401 accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg
     5461 cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg
     5521 gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc
     5581 cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg
     5641 ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta
     5701 tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa
     5761 aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat
     5821 gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa
     5881 gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc
     5941 tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt
     6001 taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat
     6061 ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg
     6121 cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg
     6181 gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg
     6241 atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga
     6301 aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact
     6361 tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa
     6421 aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt
     6481 gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca
     6541 ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca
     6601 agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg
     6661 atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat
     6721 tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa
     6781 tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga
     6841 tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg
     6901 gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta
     6961 ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt
     7021 tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct
     7081 gcactagctg catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc
     7141 tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta
     7201 cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg
     7261 ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact
     7321 accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc
     7381 aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca
     7441 ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca
     7501 cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga
     7561 tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa
     7621 gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac
     7681 tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc tttgaatcga
     7741 tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc
     7801 gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag
     7861 tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat
     7921 cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca
     7981 gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa
     8041 gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct
     8101 cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa
     8161 gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact
     8221 atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt
     8281 tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt ttaattcgtt
     8341 atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg
     8401 gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa
     8461 ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt
     8521 tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt
     8581 ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa
     8641 gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat
     8701 ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca
     8761 ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca
     8821 gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt
     8881 ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat
     8941 gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca
     9001 atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag
     9061 acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag
     9121 gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta
     9181 ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag
     9241 ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg
     9301 gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc
     9361 tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt
     9421 acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct
     9481 ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa
     9541 aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta
     9601 tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa
     9661 ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat
     9721 caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt
     9781 ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca
     9841 cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc
     9901 cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag
     9961 gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact
    10021 ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa
    10081 gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca
    10141 aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca
    10201 gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat
    10261 tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc
    10321 atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa
    10381 caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat tattttactt
    10441 ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca
    10501 gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg
    10561 catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt
    10621 tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca
    10681 gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt taaggtcatt
    10741 tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag cgaaataatc
    10801 attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct atgacggcca
    10861 atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg gtcaattgat
    10921 tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg aaatgctagg
    10981 tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct ttcgataaga
    11041 attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc ctccatccga
    11101 tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga
    11161 taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa ataacggttc
    11221 agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa tcttgcgtat
    11281 ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac tttgcggtaa
    11341 ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt cttccccgtc
    11401 catcttccac atacttcata tcatttcaca tgatacttat gctctcatca taccacaggt
    11461 tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag tgagattgtc
    11521 ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt cttctaatgc
    11581 cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa tcattttttc
    11641 aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca tttggatctc
    11701 agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac tatggaaatc
    11761 cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc gaacatcgat
    11821 gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa aagcaaagat
    11881 tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc cgccattttt
    11941 cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac
    12001 aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt atgtttcatc
    12061 tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta cattataagt
    12121 tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag gatcacctga
    12181 ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat tattagcatc
    12241 tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt cacttcgctt
    12301 tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg tgaatcacga
    12361 ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca attatctata
    12421 ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat tgatagattt
    12481 gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt cgggaataaa
    12541 tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat ccaagtagtc
    12601 gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc agcaagacat
    12661 aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc tttgacacaa
    12721 aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct attccttcgg
    12781 tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga taacgcaaat
    12841 acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct tctgtctgag
    12901 gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg gctgttgcac
    12961 tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta cgtcggatcg
    13021 gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt aatcccaaga
    13081 tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga aaaacctcag
    13141 tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc tcttctatcc
    13201 tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa gtctcttctt
    13261 ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact gcacctttag
    13321 caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact aaatccacac
    13381 cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt tcgcctcttt
    13441 gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt tttcgtgcca
    13501 tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata atcccgcagc
    13561 ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac aagtaaaaaa
    13621 aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac acaatacttc
    13681 tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata taatttatgt
    13741 tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta
    13801 caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt
    13861 cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat ggataggtac
    13921 gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt tcttcaacga
    13981 aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta tcccacaaaa
    14041 aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca tctacttgat
    14101 aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga tagacttcat
    14161 caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca gaaaaacagg
    14221 taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc acaaattgga
    14281 tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct tctgaaaggt
    14341 ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct ccagtaccgc
    14401 atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc
    14461 attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat gtttcataag
    14521 ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac catagttttt
    14581 ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca tctccaagat
    14641 cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca tatttgtttt
    14701 cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg
    14761 aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa gcgataatgt
    14821 cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct ttactatcta
    14881 tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat tatatgtttc
    14941 gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt
    15001 ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc
    15061 aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt caaggagact
    15121 catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg catcggtgat
    15181 tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg cattattata
    15241 attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact cctcatctgg
    15301 tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag gagaagcacc
    15361 gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct tgaagcgttg
    15421 ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag cagtatattc
    15481 attactatag tccatattca tacaaccctt cattttgaat ataatcaatt actttatctg
    15541 gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc gtggaactga
    15601 tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt gtgtatcctg
    15661 gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta taccattttg
    15721 gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc ggattgtttt
    15781 gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct aattcaatcg
    15841 tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg agtcgatgtt
    15901 ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc atcaaaaata
    15961 cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt gcaaggtgaa
    16021 cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag aattgaggca
    16081 tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc atcatggtca
    16141 ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt gtggaaggtt
    16201 tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt tctttttcta
    16261 atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat actttgatca
    16321 gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg atcccacctt
    16381 ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt
    16441 ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat
    16501 ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg atccagccta
    16561 gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga acaagctccg
    16621 gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca tgtttttgat
    16681 aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt gacacataag
    16741 caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa
    16801 gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac ggtgcaatga
    16861 tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata attcccggtg
    16921 tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag gtcgttcctg
    16981 gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg atttgattga
    17041 taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt ccatcgcggt
    17101 attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca ctagttaata
    17161 atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat tgtgttaatt
    17221 tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa agtactggat
    17281 tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca aaaatatcca
    17341 ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt
    17401 catccgtcaa tgagacatct tgggtctcat tataatggcg taatctgaaa caacgctgac
    17461 aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt
    17521 caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat tcttctgtca
    17581 ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg ccataattct
    17641 tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa tgggttttac
    17701 aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa tctgatcgcc
    17761 taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt tgatccctct
    17821 tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa tcgctcgcgc
    17881 gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat ttttcatttc
    17941 taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa tcaacgtatt
    18001 gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag gtgtaatctg
    18061 atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt ctcctcatct
    18121 gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg catatcagca
    18181 aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta caaaatgacc
    18241 aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata ttggcggaac
    18301 ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc cgtcaatctt
    18361 tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg ctcgccgttg
    18421 aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg cgcagtaatt
    18481 ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat
    18541 ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc atccgctaaa
    18601 cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat gatcggtacc
    18661 tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc gcgctcttct
    18721 gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg tctattgaac
    18781 ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg gggagaacca
    18841 aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat cactgttacc
    18901 ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc aaataaacga
    18961 tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa gcttgtccat
    19021 cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac gatctatcca
    19081 tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt ccatgcgggg
    19141 aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga aatcttctgg
    19201 aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg
    19261 gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc ctccggtcgt
    19321 cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt tgacagcagc
    19381 agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg cgccacctga
    19441 agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata atctggtgat
    19501 cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca tcactccaat
    19561 aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat gaagtcctgt
    19621 tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg gatctttggt
    19681 tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag ctagacgctg
    19741 ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt caccgatgtc
    19801 tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat caggaacaga
    19861 tggcgagtta gcaggtttgc tctctctatt aggattgatc cgaatatatt tctttttctt
    19921 aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg gtaaaaattc
    19981 ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac taatcaaatc
    20041 catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta aggctcgctg
    20101 catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg caatcattga
    20161 atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc gcagtcccat
    20221 cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa aatggaaagc
    20281 aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta cttcttcttg
    20341 acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta aatcgattcc
    20401 tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt ccatgaaata
    20461 aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat aattgactgc
    20521 tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg agatcactac
    20581 agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc
    20641 taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat ggcgagcagg
    20701 ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg cttcttgctg
    20761 agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa tgcctttccc
    20821 gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg ctatttccaa
    20881 tgcttcttct actgtagata atacaccagt acttcctgga atgactggga catttgcttc
    20941 ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg cacttttagg
    21001 accaataaat gtgatattgc actcttcaca catggaggca aatcggctat tttcagataa
    21061 aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc
    21121 ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg cttcatctgc
    21181 taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg tttggatccc
    21241 tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag ctatcaatac
    21301 tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg gctacttttt
    21361 taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact ttcaatatct
    21421 caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt gctttgtcca
    21481 atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat gcaactgctc
    21541 ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct tctggaaaat
    21601 gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc cgttctcctt
    21661 caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga atgatttctt
    21721 ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt tgctgaatcc
    21781 taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt tctgtgatca
    21841 ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata atgcagataa
    21901 cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt
    21961 gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat actgccgatt
    22021 ctttttcttt cattatttct ggagcagttg ttgcttgttc aggtatgaca ttgactggct
    22081 ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt gttttatttt
    22141 tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc gaggattgat
    22201 caaattgact gactaattct ctgatttcat tgatatccaa aattattcct cccagcgttt
    22261 catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg cataagtaaa
    22321 acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat cttgtttttc
    22381 tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg ccaaggcttc
    22441 gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt
    22501 atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct ggtcattggc
    22561 tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta caccagcttc
    22621 ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag gagctgtcat
    22681 atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt tggcaccgcg
    22741 agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac ccatcacaaa
    22801 accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg attctgagag
    22861 tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt ctgttcctcc
    22921 agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc caattgaatt
    22981 cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac ctacacgcaa
    23041 agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg gagaaactcg
    23101 ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa gaccaccgat
    23161 cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat ctaaaccgct
    23221 catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat ccattcgttt
    23281 gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac ccgctaaggt
    23341 aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg tcccctcttt
    23401 taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc catagccagt
    23461 aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga ccaatccgcc
    23521 atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa ctgctgtttg
    23581 tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg cttgttcctt
    23641 gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg caatggcatt
    23701 acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc ctactacgcc
    23761 agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag atacactaga
    23821 gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt gctgcgtcat
    23881 attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg catccattcg
    23941 catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga tcgatccaag
    24001 cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga tgtcaccgga
    24061 aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt ttggaatctc
    24121 tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg ccatcgcttt
    24181 gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat ccataacgtt
    24241 cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac acgcagaact
    24301 ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg gccgacttcg
    24361 atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa acgaacaggt
    24421 tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat ttccgttgtt
    24481 gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag gtccttcgcc
    24541 agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac atttagaggg
    24601 atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac tgctttttct
    24661 tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac gatgccgtac
    24721 ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc
    24781 attttcccgc ttccagctgg ggcagcttct gtcatataag cgcctctttt tgctactaac
    24841 gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata ttctcctaaa
    24901 ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg ataatacgcg
    24961 atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg ttcattttct
    25021 gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc aaatgtctgc
    25081 tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc accttggcca
    25141 ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata tgtttgattc
    25201 ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc cccaacctaa
    25261 ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa ccgttttgat
    25321 tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga tgatttggaa
    25381 taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc
    25441 agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata ccataataat
    25501 atattcctcc gatattgttt gctttttatt tttataattt aacagtctac aactccatga
    25561 gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc tgctgttttc
    25621 ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg cttgcagcag
    25681 atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc tttacttttc
    25741 gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa tcaactggtt
    25801 tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa tcaaaaatat
    25861 ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag aacggatttt
    25921 tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct gctaattttt
    25981 ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct ccatcgccaa
    26041 acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt tcaccaccaa
    26101 tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt aacgcataaa
    26161 caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct cccaatttcc
    26221 cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt gctactaaaa
    26281 taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc gccactaaaa
    26341 tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata cccgtccttg
    26401 acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta gtaataatct
    26461 tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt aaatatcctc
    26521 acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt
    26581 cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa tgctgatgga
    26641 cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg cggtcatctt
    26701 cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca gtggtcaagg
    26761 tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc ttgtacatcc
    26821 cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga gattttttca
    26881 gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa tcatttactg
    26941 tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc aaatcttttg
    27001 atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca tttttttact
    27061 atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag catttataat
    27121 ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta ttttctgtga
    27181 acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta tagtcttttt
    27241 attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg
    27301 ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg taaagcaaaa
    27361 cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc aaaaaaagta
    27421 ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac ggaattagat
    27481 gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa ccgacaaatg
    27541 aattatttca gtaattataa ttatgtacat attaccaata agggatttgt attaacagat
    27601 actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc gtctattttg
    27661 cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt gttagctttg
    27721 cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa ggaactccaa
    27781 gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag acaacaacat
    27841 ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt
    27901 acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc atcgctcacc
    27961 ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt ttgttatggt
    28021 caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta ttatgaaaag
    28081 aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa
    28141 atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga actgttaaac
    28201 aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gcttctttta ggcatcttca
    28261 acttcaaatg tcaattatcc taaaatttat tggattaagt catagatttc catcgctacg
    28321 atatcgatat tatcaaattc gtattgctga tgagtatctg tttcagttaa ttcaaatgta
    28381 tcggtcgctt tatcataagt tactgtacac ttctctacgc cttctttttc aaaacggcga
    28441 acttgcagtt cattatctgc tgactcgacc atagcctcca gtcgttttat gattgcgact
    28501 aattgtgaat gtgtcatcgg aggtcctcct tttcttattt ctgttatatt gtatcaaaag
    28561 catagcctac ttgcattgat tttctctaaa aaaactcgtt ttttatgatt ttttttctgg
    28621 tatttttcct tcaggccacc aaagtttgat caaagcttgg gttccgtcat tctcaaaacc
    28681 tttatctgcc aactcttcat atagtttcaa tgcttgggta gttgctggca atacaagatc
    28741 cattttttct gcttcttcta atgcaatctt taaatctttg ataaaatgtt tgacaaaaaa
    28801 accaggtgtg taatcttctt ttaaaatacg aggagaataa ttgcttaaag accagttagc
    28861 tgcacttcca cctgaaagcg tctcaatcac tttctttaaa tcaagtccgt ttttttgtgc
    28921 atagacaagc atttccgtca ttcctgtcat cgtacctgca atcatgatct gattagccat
    28981 ttttgtatgc tgacctttcc cagcagaacc atgcaatgta aatgtcgtac caaattcttt
    29041 gaataaaggc agtacttgat cataaacggc ttcttctcca ccgaccatga tcgtcagtgt
    29101 accattcttt gctcctaagt caccaccgga taccggtgca tctaacgcat ctgcgccttt
    29161 ttcttttgct gtttttgcaa ttttttctgc caggcttggt gtacttgtag tcaaatcaac
    29221 taaaatatgt cctgagatat ctgcagaaaa aatccccgac tgtccatagt agatttcttc
    29281 tacatctttg gggtaaccta ccattgtaaa gagaatatta ctttgttctg ctaccgcttt
    29341 tggtgtatct gcccagatag ctccttctgt tactaaagga tcagcttttg cttttgtccg
    29401 attatagaca gctacttcgt gtcctgcttc tagtagatgg cgtgcgacag cacttcccat
    29461 gacgcctgtt ccgataaaac caagtttcat tttttcgacc tcctaaaaat caaaaatcct
    29521 tgcttctatg ctagtatacg ctagaaacaa ggattttttc ataaaaaatg tttgaatttt
    29581 atcttttaaa tgaatttaat tttattttga ttcgttcaat cgagtcttct tcttcttttc
    29641 ttcgcatctc ttcttcatga taaacaacca tgtcttgata aaattcgatc atagcttcgc
    29701 cgaaatgtgt agatgaaata tcataaagtt tagcttctaa aacttttgga tctttaggaa
    29761 cttcactgga aatataatca ataagtgctg gtgcaaagtc ttcgtcggtt tcaaaagttt
    29821 tccctaatga aggatgatcg aacaatcggt tcaaataagc atttccttcc acgacacaag
    29881 gtgttccggc tgccatcgct tctgtataag taagcccttg tgtttctgaa gtagaagcac
    29941 tgacaaaata atctgccgct ttataataaa gtgctacatc attgttgtcc acttctcctg
    30001 tgaactgcac aaattcttct aactgcagtt ctgagatcaa ttcctctaac tcttctacat
    30061 aaggtccttt tcctacgata actaatctgg cgttaggata agcggagatg acctgaggaa
    30121 acccattaat caaggcttgg atatttttct cataagaaat cctgcttaaa gaaagaatca
    30181 tcaaatggtt ttcctgaaga cctagtgact cccttaattt gctgatatcc tgctgagtga
    30241 tatcagaacg tttgaatttt tcaatgtcga taccagtagg aatcacgcga agcggtgcta
    30301 ttacaccata gctgcgtaat gtatcaataa cacgttcact tggacaaact acacctgtcg
    30361 tatgatttgc aaataatcta gacaagtatt ttacatggga ttgcctgatc acttttcctt
    30421 taccgatata atgaaggtag tcttcataca tcgtatggta agtatggatc agaggtattt
    30481 tcaacttctt agctaccatc ttcccaagta tccctgctcc gaattctgta tgggtatgaa
    30541 taagatctag ttctagttct ttggcaacca aataagcata ccacatgcct ctaacaacaa
    30601 ctcttcgatc cttgaaagaa ataaatggta cacttggcat tcgaatcaca tctttttcca
    30661 acttatccgc attaggatcg gtcgttgtaa aaatatagac ttcatgccca tgtctttcta
    30721 attcttgttt taatgttttg atcgatgtag caactccgct gacttgcgga aagtaggtat
    30781 cggtaaaaaa gccgattttc atgtttttcg cctccaaact agtccttgac ctgatttact
    30841 ttttttaatt tgctagagac tttttcattc agtacacttt cgtatacatt tttaagttct
    30901 tcaccgattt tattgatact tcgctctttt gctgtttgat aaccggcttg tgtcgtactc
    30961 ggtagttctt gattggtaat ctgttggatc agtcggataa attcttcgtt tgtcttcccc
    31021 atatagcagt tcttgctatc ttctaaccag ccattataca ctggaatatc tcgaacaagt
    31081 acgttttgtt tgcttgctaa agcttctaaa acaacgatcc cttctgtctc ttcataagaa
    31141 gggaaaaaga atagatctgc acctgaatac gcaccttcta tgatctctcc acgaatgtaa
    31201 ccaggaaaca ggacattttc aggatgatct tcttttacaa ttttccggat gttccgagga
    31261 atcgagtaca tgggaacatg accaaaccaa ataaatgtgt attcgggcat cttcttagca
    31321 atttctacga aatcaatgat tcctttacgt tcgaaaaaca aaccgacaca aatgattact
    31381 ttttgatccg gagacagttt gaaatattct ctgaactttt gttctttcat tggatcaggg
    31441 tgatatttct ctaagtcgat cccattggaa atagcttgaa tcggcacacg gataccatag
    31501 ctttccaaca actgttttga ataaggtgtt ggcgtgatca gatagtccgc tttttggtac
    31561 aatcctacca gatatttttt tacgatagga gaaagctggt tcgatccgat aaaggaattt
    31621 cggaaatctt cctctgtaga atgcgcatga tagacaactt tttttccttc tcttctggct
    31681 ttattgacca tattatgact attgattccg tatgtattga tgtgcaggat gtcataatct
    31741 tctttttcat ctagtgtata agagataccg acttccgtca gagcacgttt ttgatgatca
    31801 agcgcacgcc cgattccaga ttttgcaagc atcttttcac tttcaaaata taataatacc
    31861 ttcaccttac gtcccccttc acttatatag tataacatag cctacaaact gtcactcaaa
    31921 cttaagccct atttttgtct attcttactg acaaaaacgt tttcttaaaa aagaacaaaa
    31981 aaactggcgt agctctttta caagccacac cagcttttga tacgttgttt attttacata
    32041 ttcatgaaca agttcaacga cttcttccat cgtatcgcat tctttcaatg cacgatcagc
    32101 aagttcagcc attttcgctg tgtccaagcg tttcattaaa ccacgcgttt taagaataga
    32161 tgtcgcactc attgagaact catctagacc catacctact agcaatggaa cagctgtttg
    32221 atctccagcc atttctccac acatacctgc ccattttcct tcagcgtgtg ctgcatcaat
    32281 tacatttttg atcaaacgta aaattgatgg gttatatggt tggtacaagt aagaaacgcg
    32341 ttcgttcata cggtctgctg ccattgtgta ttgaatcaaa tcgttcgtac ctacagagaa
    32401 gaaatctact tcttttgcaa acttgtctgc taagactgct gctgctggaa tctcgatcat
    32461 gatgccgact tggatcgtat cagatacttc cttgccttca cttatcaatt tttgtttttc
    32521 ttcttcaaag atcgcttttg ctgcgcggaa ttctttgagt gtagctacca tagggaacat
    32581 gatacgtaaa ttaccatgaa cagatgcacg aagcaatgca cgcatttgtg tacggaacat
    32641 ttcgtcgcct tgttcagata aactgatacg caatgcacga tatcctaaga atggattcat
    32701 ttcatgaggt aattgcagat atggtagttc tttatctcca ccgatgtcca ttgtacgaac
    32761 aactacaggt tttccttcca taccttctaa tacagctttg taagcttcat attggtcatc
    32821 ttctgttggg aagtctggag aatccatata caggaattca gtacggtaca gaccaactgc
    32881 ttctccacca ttattatgga caccaactag atcttttggt gttccgatgt ttgcagctaa
    32941 ttcaaaatgc ttgccgtcag ctgtgacagt ttctgcattt tttaattttt cccattctgc
    33001 tttttgtgct gcgtaatctg cacctgcttt ttcaaattca gctttttgtt catctgtagg
    33061 atcgatgatt acatcgcctt cgatcccgtt gacagccaaa atatcgcctt ctttgacttt
    33121 agcagtaatt tcttttgtcc caacgattgc tggaatttct aaagaacgtg ccatgatagc
    33181 agaatgtgaa gtacgtccac caatgtctgt tacaaacgct tttacaaaat tgcgatctaa
    33241 ttgtgctgta tcacttggag tcaagtcatg tgcaacaacg accacttctt cgttgatcat
    33301 tgatggattt ggtaaagtaa cgcctaataa gtgcgctaaa atacgtttag caacgtcacg
    33361 aatatctgca gcacgctctt gcatataagc attatcttcc attgcttcga acataccgat
    33421 atacatatcc gttacttctt ttagcgctga ttcagcattt acgctgttgt ctttaatatt
    33481 ttgcttgatt tgacctacca tttctggatc tgaaagaacc atcaaatggg catcaaatac
    33541 ttgcgcttct gcttcaccta agctttgcgc tgctttttca cgaatttgct gcaattcttc
    33601 agtagattta gctaatgcag catccaaacg agcttcttca gccgcagtat cttctactgt
    33661 tgccttactg aatgttaaat ccggttgaac tagcaggtaa gcttttgcaa cggctacacc
    33721 gtcactagcg gcaatccctt ttagcatttc aaccattatt cagataagcc ttctttcttc
    33781 attgtttcaa cgattgctgc cattgcatca gcttcgtcag caccttctgc agtaatagta
    33841 acatcagaac cttgacctac acctaaagac ataacgccca tgattgattt caagtttact
    33901 gatttacctt tatactctaa gtttacatca gagttaaatt tgcttgctgt ttgtactaat
    33961 agtgtagctg gacgagcgtg gatcccagtt tctgctacta cgtgaaattc tttcttttcc
    34021 atattaatcg gtctccttta agtgagtgtt gtttttatgt gagggttttc ttttttagta
    34081 attcagagaa tcctaacaaa tgccaccctt tcatctgtaa agattaacat tttttttcgt
    34141 tagatacaac agttttcatt caatttcaga taaaagtatt caaattattc cggattcttt
    34201 ttcatccttt tcttcacatt ttataacaaa cgtgtttatt tcctttttct tgtcgctttc
    34261 tcttctcctg acgtttatca ttctttcctt tgaccctctt tgttttcata tgaaacgaat
    34321 tacttgcttc cctttttatt tttagtataa taagagtcaa aggtcaggaa tggtcaatag
    34381 agatttcacc ttttctacct cataaatctc ttaaaaaact aggggttatt cttttacctc
    34441 atgaaaaacg agatgtaaaa tatggatgta cccatttttg tgaaaggacg tgaatgattc
    34501 atgctttgtc aaaactgcgg caagaatgag gcaacgattc acttgtatgc cactgtcaat
    34561 ggtcagcgga cacaactcga ttactgtcag agctgttatc aaaaaatcaa aaatcaacaa
    34621 aatggaggtc tgactaatat ggcccaaaat gatccatttg gatttggcag tttggacgat
    34681 cttttccgtt ccatgtctcg ccaaatgcaa cagcaaggaa attatgagca aacaccacca
    34741 acacaatttg gcggaaacaa caacttcaat ggtggtcagc cacctcaagg caatacagaa
    34801 ggtttattgg gagaatacgg aatcaatatc acagaacaag cacgacaagg tgatatcgat
    34861 cctgtgatcg gacgcgatga cgaaatcaaa cgagtcattg aaattttaaa tcgccgaacc
    34921 aagaacaatc ctgtcttgat tggagaacca ggtgttggta aaacagcagt tgttgaaggt
    34981 ttagcacaaa aaatcgtaga tggtgatgta ccgcaaaaat taatggacaa agaagtcatt
    35041 cgtttagatg tcgtatcttt agtccaaggt actggtatac gaggacaatt tgaagaacgt
    35101 atgcaaaaat tgattgaaga gatccgtcaa gcagaaaatg tgatcctatt catagatgaa
    35161 gttcacgaaa tagtcggtgc tggttctgct ggtgatggaa atatggatgc tggtaatatt
    35221 ttaaaaccag ctttagctcg aggagaattg caaatggttg gtgctactac cttaaatgag
    35281 taccgcatca tcgaaaaaga cgctgcttta gaacgtcgta tgcaacctgt acgggtagat
    35341 gaaccaactg tcgaagaaac gatcagtatt ttgaaaggtt tgcaaaaacg ttatgaagac
    35401 tatcatcatg taaaatatac tgatgaggcc atcgaagcag ccgcaacttt atccaatcgt
    35461 tacgtccaag atcgcttctt gccggataaa gccatcgatt tgctagatga aacaggttct
    35521 aagaaaaact tgacgatcca aatcgttgat cctaaaacaa tcgagaaaaa actgcaagaa
    35581 gcagaagaac aaaaagtttt agcttctcga gaagaagatt tcgaaaaagc agcttattat
    35641 cgcgatcaga tcaataaact tcaaaaaatg aaagaacgtc agctcactga agaggaaaca
    35701 cctgtgatta ccgaaaaaga tatggaaaaa atcgttgaac aacggacagg tattccagtg
    35761 ggtgaattaa aagaaaaaga acagacacaa ctgaaaaact tagcagatga cttgaaagct
    35821 catgtcatcg gacaagataa cgctgtagat cgagtagcga aagcgattcg ccgaaaccgt
    35881 gttggattaa acaaacagaa tcgtccaatc ggctccttct tatttgttgg accaactggt
    35941 gtaggtaaaa cagagcttgc gaaacaactt gcctacgaac tattcggttc acaagattca
    36001 atgattcgtt tcgatatgtc tgaatatatg gaaaaacata gtgtctctaa attgattggt
    36061 tctcctccag gttatgtcgg atacgaagaa gctggtcaat taacagaaaa agtacggaga
    36121 aatccttaca gtcttgtctt gttagatgaa gtagaaaaag ctcatccaga tgttttgcat
    36181 atgttcttgc aaatcttaga tgatggtcga ttgacagatg cacaaggaag aacggtcagc
    36241 tttaaagata cgatcatcat tatgacaagt aatgccggaa ctggtaaagt agaagcaaac
    36301 gttggttttg gtgcggcacg cgaaggtgtt actcgttctg tattgaacca attaaacaac
    36361 tactttacac cagaatttct gaaccgtttt gacgggatca tcgaattcag tgcattatca
    36421 aaagaaaact tgatgacaat cgtcacacta atgctagacg atgtcaacca aatgctggca
    36481 gcacagcaac ttcacatcga agttcctacg aatgtcaaag aaaaattagt tgacttaggc
    36541 tatgatccat ctatgggtgc tcgtcctctt cgacgcacga tccaagaaca aatcgaagat
    36601 ggtatcgctg aattttatct tgatcaccct tctatccatg agttgaaagc aaaactggat
    36661 aaagatggaa aaatcatcgt tacttctaaa ccagaacgtt tggctaaaga atcagccgaa
    36721 gaaacggctg aataaatcca gttttatagc gtgagacggt atttctttga gaaatgccgt
    36781 ctcattcttt tttcagaaaa gtcaaaatac tgttcatttt ataaaaaaga aattataata
    36841 agagaaacaa gcaattaaaa atacataaaa gaggtgagcg ccatgaaatt ggtaaatgta
    36901 acaaacagcc attcccgtct tgtattaaac cagcttgaaa acactgatgc ccatctagtc
    36961 aaggtctaca cagccggaaa taccactatt gtctacactg aagcccctca acataatgaa
    37021 attctgctga tcaatgacaa acgtaaaatc cagccgaaag aaatcgaaga tgctaaaaat
    37081 tatttcttag ataaaatcaa agacgccgtt taccacgagg atgaaatcaa agtaatcgaa
    37141 ttggcaggcc ttgttgaaat ctcgattcca aaacatgaat taggcgctac attagccgtg
    37201 taaacaaatt ttgaacataa taattaaaga gcgtgggaca aaagtaaa
//
LOCUS       DAPBCS010000027        33160 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPBCS010000027 DAPBCS010000000
VERSION     DAPBCS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.027"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            241..825
                     /locus_tag="RD521_001557"
     CDS             241..825
                     /locus_tag="RD521_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771298.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            836..1081
                     /locus_tag="RD521_001558"
     CDS             836..1081
                     /locus_tag="RD521_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771299.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1087..1809
                     /locus_tag="RD521_001559"
     CDS             1087..1809
                     /locus_tag="RD521_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771300.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            1940..2683
                     /locus_tag="RD521_001560"
     CDS             1940..2683
                     /locus_tag="RD521_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771301.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2689..3054
                     /locus_tag="RD521_001561"
     CDS             2689..3054
                     /locus_tag="RD521_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771302.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3070..3600
                     /locus_tag="RD521_001562"
     CDS             3070..3600
                     /locus_tag="RD521_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771303.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3762..4070
                     /locus_tag="RD521_001563"
     CDS             3762..4070
                     /locus_tag="RD521_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771304.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            4066..4485
                     /locus_tag="RD521_001564"
                     /pseudo
     CDS             4066..4485
                     /locus_tag="RD521_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4622..5422
                     /locus_tag="RD521_001565"
     CDS             4622..5422
                     /locus_tag="RD521_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771305.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            5459..5641
                     /locus_tag="RD521_001566"
     CDS             5459..5641
                     /locus_tag="RD521_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771306.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            5752..6069
                     /locus_tag="RD521_001567"
     CDS             5752..6069
                     /locus_tag="RD521_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771307.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            6072..6304
                     /locus_tag="RD521_001568"
                     /pseudo
     CDS             6072..6304
                     /locus_tag="RD521_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6419..6898)
                     /locus_tag="RD521_001569"
     CDS             complement(6419..6898)
                     /locus_tag="RD521_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771308.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            7018..8355
                     /locus_tag="RD521_001570"
     CDS             7018..8355
                     /locus_tag="RD521_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDT7771309.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8378..8905
                     /locus_tag="RD521_001571"
     CDS             8378..8905
                     /locus_tag="RD521_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771310.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            8892..9455
                     /locus_tag="RD521_001572"
     CDS             8892..9455
                     /locus_tag="RD521_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771311.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            9877..10614
                     /locus_tag="RD521_001573"
     CDS             9877..10614
                     /locus_tag="RD521_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771312.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10631..11047)
                     /locus_tag="RD521_001574"
     CDS             complement(10631..11047)
                     /locus_tag="RD521_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771313.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11228..11443
                     /locus_tag="RD521_001575"
     CDS             11228..11443
                     /locus_tag="RD521_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771314.1"
                     /translation="MNLDKYNLYTAREASLILGKGRNYVTNIINYYPNRLPKGSFKKV
                     GDKTYIITEEGIEALRSQTENKNKKKE"
     gene            11440..12687
                     /locus_tag="RD521_001576"
     CDS             11440..12687
                     /locus_tag="RD521_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7771315.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            12955..15345
                     /locus_tag="RD521_001577"
     CDS             12955..15345
                     /locus_tag="RD521_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDT7771316.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15394..17229
                     /locus_tag="RD521_001578"
     CDS             15394..17229
                     /locus_tag="RD521_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDT7771317.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17298..18161
                     /locus_tag="RD521_001579"
     CDS             17298..18161
                     /locus_tag="RD521_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7771318.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            18151..19260
                     /locus_tag="RD521_001580"
     CDS             18151..19260
                     /locus_tag="RD521_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7771319.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            19599..20399
                     /locus_tag="RD521_001581"
     CDS             19599..20399
                     /locus_tag="RD521_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771320.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20487..21293
                     /locus_tag="RD521_001582"
     CDS             20487..21293
                     /locus_tag="RD521_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771321.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            21525..22394
                     /locus_tag="RD521_001583"
     CDS             21525..22394
                     /locus_tag="RD521_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDT7771322.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            22414..22761
                     /locus_tag="RD521_001584"
     CDS             22414..22761
                     /locus_tag="RD521_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771323.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            22774..24036
                     /locus_tag="RD521_001585"
     CDS             22774..24036
                     /locus_tag="RD521_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771324.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            24116..25492
                     /locus_tag="RD521_001586"
     CDS             24116..25492
                     /locus_tag="RD521_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDT7771325.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            25489..26118
                     /locus_tag="RD521_001587"
     CDS             25489..26118
                     /locus_tag="RD521_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771326.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            26137..26616
                     /locus_tag="RD521_001588"
                     /pseudo
     CDS             26137..26616
                     /locus_tag="RD521_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27118..27171
                     /locus_tag="RD521_001589"
                     /pseudo
     CDS             27118..27171
                     /locus_tag="RD521_001589"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27175..27774
                     /locus_tag="RD521_001590"
     CDS             27175..27774
                     /locus_tag="RD521_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDT7771327.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            27790..28266
                     /locus_tag="RD521_001591"
     CDS             27790..28266
                     /locus_tag="RD521_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771328.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            28283..29185
                     /locus_tag="RD521_001592"
     CDS             28283..29185
                     /locus_tag="RD521_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771329.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29258..29452
                     /locus_tag="RD521_001593"
     CDS             29258..29452
                     /locus_tag="RD521_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771330.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            29557..29985
                     /locus_tag="RD521_001594"
     CDS             29557..29985
                     /locus_tag="RD521_001594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771331.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            30220..30807
                     /locus_tag="RD521_001595"
     CDS             30220..30807
                     /locus_tag="RD521_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771332.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            30807..31706
                     /locus_tag="RD521_001596"
     CDS             30807..31706
                     /locus_tag="RD521_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDT7771333.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31706..32620
                     /locus_tag="RD521_001597"
     CDS             31706..32620
                     /locus_tag="RD521_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771334.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            32672..32887
                     /locus_tag="RD521_001598"
     CDS             32672..32887
                     /locus_tag="RD521_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771335.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12548 a         4527 c         6620 g         9465 t
ORIGIN      
        1 gtaattgaca gctattgcag aacgataatt tccctgataa aaactacttc taacttcttc
       61 taataatgga ataattctag gatttttaac tttttttatt tcatcatcta atataaacat
      121 ataaatctcc tttttttatt tgtatgacaa gtttaccata aaaaaaatta aaaagcgaga
      181 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
      241 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
      301 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
      361 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
      421 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
      481 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
      541 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
      601 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
      661 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
      721 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
      781 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
      841 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
      901 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
      961 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     1021 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     1081 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc
     1141 cttacagcat gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt
     1201 acgatggtta ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa
     1261 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa
     1321 gtttataaca actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg
     1381 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     1441 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     1501 gatattacca taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat
     1561 ggtgtacctg agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     1621 acgccaacag ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta
     1681 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     1741 gatttgggag catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa
     1801 gttaaataga tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     1861 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     1921 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     1981 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     2041 taccggttac aataatggtg caaatgggaa gtggttaaag gcaacaagta ttcgtgaact
     2101 tagaagtgaa gtagaaaaaa ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa
     2161 gggaaataat tcttttgtaa ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct
     2221 gaaaattgaa gaatactatg aaattgattt acctgaaaaa ttaagaatta ttgatcaacg
     2281 ttcagaagaa aaatggaaaa ctgaaatata tccaagctta aatgactaca agacatcagg
     2341 aaagcccatg tataactaca caggcttttt aacctacaca caatacaaat atgcctttcg
     2401 caaattaaaa ggcattaaac aagctgaaat tgccctgtat gagatgaaaa aagtggatag
     2461 attgaaattg attcaagatt tagtgcaaaa tacatggaat caagcgattg tcgagctaga
     2521 aaaagaaaat gggaaaattt taactaataa atatctgatc gtaaatgaaa ttgaagaatt
     2581 aattggttta ttttcatgtg aaaaaggtga agtcttcttg aagtcatcac caacctattc
     2641 aattttggac agtccgtatc agtttataca ggcaaggaga tgagttagat gtctagcttg
     2701 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag
     2761 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     2821 aaaactttag aggaatatga tcggcagttt aatcagatac atcacaatat cattttgctt
     2881 gttgagcagg caaagcgaag gtttgaaaaa gcggaatatg atctattaga tcaaggaatt
     2941 gtatgcttgg ataagggatt gcgaagatat tatggtgcag agttaaaaaa gcaaaatgta
     3001 gatagaaatg cactaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat
     3061 agaaagcaaa tgtcacaaaa cattagtgct gtaaaacaat taatagaaga agatttagag
     3121 accacagttc agagattagc ggaacgaatc cattttgcaa attatcaaac catctctaac
     3181 tggtcaagca ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga
     3241 aaagaatatg gtgttgatcc gataaaaatt ttcgagaaga ccatgattta ttactatcat
     3301 ggaacaccta acgaagtgtt agaacatctt ttggaagagg caaaagaaaa atcatcaata
     3361 ttagagctat cgcaaaatga caaagttatt caagtgaaat gcgttgatcc aacaggaaag
     3421 attattgtcg gttttgatat tgaaagaaaa tccaaaatag attttattac acaattaaaa
     3481 aagataaata atttagatat tctaacgctt agaccagatc atgaacagtt gtttatgagt
     3541 tttaaaaaac aattgaaaga aatacaaaaa agatataaca aggtgtttcc taatgcgtga
     3601 aaagctcaaa gaaatcagag ttttttggat aattaacaaa gaatgtatca tcttatatac
     3661 tcttggattt cttagcatga tcttgttttg gtggttatat ttaaaacagt aattggagag
     3721 ggtcaagagg atttttatca agataaggat gtggaaactg catgtggaat aatagattaa
     3781 aaacaggatt gttattaata gttatttcct gcgctatgat gattggtatg aggatacaaa
     3841 gggaacaatc ctattttgaa gtttctgcta acaacgtaat agaaaaatgc tattacggtc
     3901 aacattattg gagtgaggaa gtcagagaaa atatagatcg tgaatatgtt caaagaattg
     3961 tttgggatgc atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca
     4021 gtgaaaaaga taatcaaaaa ctctcagatt tgatgatgaa aaaagtatga ctattattac
     4081 gaaagagaaa cagagtgcta tattcgaggg gaaaaagcct acaacaaaag tgactattgg
     4141 ggtgacaagg cgaagcgatt aactgcaaat tttagagtgt atgaatcaga gtttataaac
     4201 ttgacctttc ttaatagtgt ttttattgat tatgtaatta ctacgaaacg cattggaagt
     4261 tattcaagta cctacgcatc cttagttcca atgttaaact cgatgagccg tttcttaaaa
     4321 gaaagagaaa agaaagagct acagtatatc aaagaaatca tgccggattt tgaaatgcac
     4381 gaatggcaaa ttgctttatc agattggaaa ttggcgaata aggttcgtag aataacacct
     4441 tttcaagcca agcgcttttt gagagctatg aaatttgtgc aataaaaaaa gccaccagac
     4501 aagttgatgg agactaacac aattaccaac agttgtaatt gtgttagctt acaaattaat
     4561 tatggtgttt agtcaatccc aaattgtgaa atggcaccaa taattggaaa ggaaaaatga
     4621 aatgtctgaa gatttacgag agctgaagtt acgaaagaag cagtatgatc agtatttttc
     4681 cggtttgttg aataaacata ttgaagaagg acttgatcat gattttggaa aagatgaata
     4741 ttgtttatat tgcgaacgtt acaatgaaaa aatgtctgaa tatctacagc tgaaaaaaga
     4801 gatacagaaa ttagagaaaa aagtacatgc ttggaagtat aaagattcca tgtctggtta
     4861 tgttgaatct caaaataagc caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa
     4921 tggggaagag ttaattgtat tagcgtacag tgaagaagag gttcgagaac tcgttgaaac
     4981 acctgtcagg aaaaaggttg aaacaataaa tgagatagac gacagccaat acaatagcat
     5041 ctatattgaa acaagtcatg gtggatataa gaaattagca actttcctta gaagttctaa
     5101 agttgggata atttctaaaa aagacataag tgctgttacc gatagttttt gtactttatc
     5161 tgttcctaaa gcttatttcc aatacgatag taaaagattt gaacgcttag agaaattctg
     5221 tagaccgtat actgtattga aaactaaaaa atttaataaa actaaaagag tttctataca
     5281 gttaccgact ggtcaaatct cattaggtga aaaagactat ctgataaaag taggaaaaga
     5341 gttaattata aaagtaccgg aacacttatt taaagaaaat tttgagatac gtgaaacctt
     5401 ggtatggaaa gaagaaaaat agtttggaag acatagaatg gtataagggg aaattgaaat
     5461 ggcagtaaat aaaatattgc aggtactaat catagacgaa tttggcggag gagaatggtt
     5521 tgatgtgcag tccatagact ttgaagaaga agtcgtagtt atagaaaata tttacggcga
     5581 agtatattca tattcgcttg aaggtgagaa tattgaggat ttacggttga ttggtgagta
     5641 gaaaaaaaca gaaatatcaa aatggagata aaaattaata gcatgtaaag atagtcatgc
     5701 ttgggttaaa aatgttaaaa ttcaaaaaaa tgagtcagaa gggataaaag tatgaaaaga
     5761 gaggaataca aacaaagatt aaacgaactg cttgaagagg acgaaacatt aactcatggt
     5821 tcgcctgatg aaattctata catgattgat aatatggtga tctttggtgg atatgaactt
     5881 ggtaataggt cagttgatca taatatttta gaatttgatg atgtatcatg ggaagaaatt
     5941 cttgattggg gaattttggc ggtacctgaa actaaaacct atattagtga cactatggtt
     6001 cctttttttg aggaattgga ttataaaaga cttcccaaaa atgaaaatca tatactggga
     6061 ggaaactaac aatgttaagt gacttattta ttttactgat ctatctaggt gtaagtgaaa
     6121 ttattgcgta ttttttgttt gtcattgcta attatggtat ttccagtgca atcgttcaaa
     6181 tgtttagatt accagtaagt gaactaaaaa aatgatgaga ttatcagcat atcttttaat
     6241 gtttttgaat gtggtcacgt taatctttgt agtaatcggt gtgactttcg atttaatgga
     6301 atagtgggaa atctataatg gaaaaaagag acacgaatga acatattttt tgaatcaata
     6361 ttgttgatgg tttttgcgat cagcgcttac cagaaatcta aaaaagagta atgattgttt
     6421 aatcatcact ctttttttgt agggaaattg aaatatgtgg agcttgtatt ggtttagtta
     6481 ggattgcttg gctataagga gctagttctt tatcaaaacc atcaaagtag gttttcagta
     6541 gcgatttaac tgatcgctca tcttgtttga tttggataat ttcagcatcc aaatcggttg
     6601 tgagatcagg agcttttgat gcgattacct gatttttttt atcttccaac tctcttaggc
     6661 tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat tccaacgtga gttgcaattt
     6721 taacagaagt ttcgtccgta ttgtcatcaa aagaactcat tggaatatca agtagtaacg
     6781 aggatgtttc ttttactgac tttttcttta cagcattttt tgtttttcca tcttttttgc
     6841 tctttactcg agatgttttt ggagctagtt taggtaaatc acttgtttta tcagtcattt
     6901 attagctttt cctttctatt aatttatgtt attatattat cataataaca taaaaaagta
     6961 cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac attgcttatg
     7021 ttaatggtgc aatttacagg gattgcaatg agtgtaatca caataaatcc gcaagtgata
     7081 catgctgatg ggatagctac ttatgatgca caatttaact gtactactga aggtagtggg
     7141 tcaggaacag atagcgatgg aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc
     7201 aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc aggtgctagt
     7261 attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg agcagtagaa
     7321 gggtattacg gagatggaca gagagaagtt gacttcatca aacaatttac tgatggtctg
     7381 atgggtattg gctatggtca atggacagct gggcgacata cagcattagt tgattggtct
     7441 aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt tatgttcaaa
     7501 ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc tgatgatgtc
     7561 gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc cgaatcagct
     7621 gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt
     7681 atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc aagtaccgga
     7741 gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc ctgtgcaact
     7801 acggatgacg ctaattctgg ttctggatca attgggaaag aaggaaaaat taacggtaaa
     7861 aaaggtcaag taatagcgaa tatgacttat gaagaagctt tgaaaaaata tgggaaagcg
     7921 atcacgctac caaaattcaa agaaccagat tggtcaactt caccttttaa cggaatatat
     7981 caaggtcagt gtacagagtt gacgtgggct tatatgagcc aattatacga tgggaatcaa
     8041 ccgactactg gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg agcaaaaata
     8101 acgaacaaac caatcgtagg ttatgggttt agtgcttcgg atggatggtt atgggctgtt
     8161 ggagatgcag ggcatactgg tgtagttata gcggtgtttg aagatggatc atttctatgt
     8221 gctaacttta atgttccacc aacccaagct ccaacaaggg gagtttcggt aacgttgatt
     8281 gatggagtgg acggatcaga taatgtgcat ttctttagcg gtgtaggaaa agctaaattt
     8341 gaaatcgaaa aataaattgg aatgaaggac gatatagatg aatagtaaag tgaaagatac
     8401 aattatcatt tctttgcttt ctggtatgtt attagcactg cttgttggaa tcctattctt
     8461 caataagcag aatcaggaca tagaagctga aaaacacagt gtgcatattg aagataagct
     8521 gacattcttt aaagaggtta aaccaaatga ggtggatgga ttacggaaag atttagaaaa
     8581 tgatattcaa gaaatgttga atggatcagt tcaaaataag tttgcagatc aatcaggtga
     8641 agaatttttg agaaaactat ttattgtaac aggtaaacca ccaattactg ataagagtag
     8701 tgagaaagaa aaaatcgagt actataagcc ttttactttt aagctaacaa actttggaat
     8761 taagacatct agtagtgggt tgtatgaagc attgaccgat tttgaagttc aatacaatgg
     8821 aaaaaaagtg tatgatacca cattaaccat cacactaaat aatgataaga aagtgactgg
     8881 gggtgctttt tatgcaaaat catgaaatca ctactgctaa aaaggcgatc ggaatacttt
     8941 ccataattgt gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct tatgataata
     9001 atgtcaaaga attggaagac cttaaaagta aacaaatcga agtagtcaag aataaccaga
     9061 aagtgaaagc tatgcaggaa gctcaataca aagaattggg aaatgctgaa attaaggaac
     9121 aaagtaggaa attctttaat ttattttaca actggaattc atgggaaaaa tatacgaaga
     9181 atatgaagga aatacaaaaa gaatttcctc agatagtaaa gtctagtcct gttgatattt
     9241 ctggcacgat ggtaggcact ggtaatagtc caatatctag ctatactgct gattactata
     9301 caacaaatca aaaaggtaaa atggttgaat tgattacaca gacaagaaat actccaacaa
     9361 cagcaacaac aacaatttgg cgtggagtaa caagcatagg agaaaatcaa ttgttttatt
     9421 tggaggattt agatagctat gtaagagcag agtaaaataa aaaaagagct aacggtacca
     9481 acgttagctc tttgtgctga cgatgataac aaaacctacc gaaaaaggat tgtttcatca
     9541 caaacacaaa cagaaaagaa ttgataaaaa acccgaacct cagaattttt attaatcaat
     9601 tcatttagat tataataaat tgagctgaac attacaatac gtatatgttg taaaacgtaa
     9661 aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa aactattaaa gacaatatat
     9721 atggtttatt tgacataaga aaatggtcta gatgataatt gtaatataga attgtagact
     9781 taaataaagc ataaaatacc ttttatgata aggtatagct cttaatttta agcagtttta
     9841 gatataagat tgaaagtaaa gtgggggaga ataataatgg cagatcataa attagaaaag
     9901 ttattagaag aaacgtggaa tccaaaagaa tttagtgaat tttttatgga gaattttgaa
     9961 actgacttag cagtgattgt taaagatgct cttagagagc aagggtatcc tgaaactgca
    10021 aactatataa acattaattt tactttgtat actgaaaata aaggaacttg ggatttttgg
    10081 gctactttag caaataagga gttaagtgat aaatcagaca caggaattcg taactttttt
    10141 gaatcaaaca gagatgatta tatgtatgct aacaatcaaa ataaattgaa ctttagagta
    10201 gagtttgatg aaacacctga agaatttata gaaagacaac cgcctaaaga aaatgtagca
    10261 aaggtattgg aagatcgttg gaattcagat gaaatagttt caacaattag cgaactaggc
    10321 ggacaatatg aaccgctagt agaagcggtt agagaagagc taagactgaa taagtttcct
    10381 gatgttcaaa atattgatgt ttctcaaatt gaaataaacg tcaagatcac gaataaactt
    10441 gattatggta gttgggcaga tatagcatta gaaaaataca tctactcaac gttaaaagag
    10501 tttatcgaaa atagaatgga tatcatgtat ctacagcatc cacagtattt aaactttggt
    10561 gttgaaatag ctacacctct tgaagaatgg aaaatggagc aagggcttga ctagcctttg
    10621 ctccattttt ttattttaca aatgaataca gttcgtcgtt tttaccaaga atgattgttt
    10681 gcccaacttt gagattgtca ggtgatcctt cagcggtttg taaggagaac gttccattga
    10741 acttatcgga taccgttata ggtagcttta attctgatcc ttcctcatct ttgagaattg
    10801 ctgtttttcg ttcatcaaaa taaacagtct ttattaaata atttttcgta gtgatctcag
    10861 cagatttgtc tttttttgta gtagtaccag tttctttaga attagaggta ttttctacga
    10921 ctattccatc tttggcattt gagcttgtgt ttatttgatt gaatattttc agcgacaaga
    10981 taacgctaag aatagtcaat atagaaacaa gtgtaattaa tactgataat ttcctttttt
    11041 tgttcatttt ttcttaacat ccttttttgt tttgattagt tagatattcc ttatctaaca
    11101 agccttttta ggctattaaa tgaagataaa ccccaaaaaa atattgcaat tatgacagtg
    11161 caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa tcgcaaacaa
    11221 aaagaaattg aacctagaca aatacaactt gtataccgca agggaagcaa gtttgatttt
    11281 gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc gtttgcctaa
    11341 aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag gaatagaagc
    11401 attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa gagaaattcg
    11461 cttggtatgg ttttattggc atcagctgta cttgcggggt tatcagtacc agtaacctca
    11521 catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac aggacaatca
    11581 agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct taaaagctta
    11641 ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt ggcaaaagga
    11701 gaacaacgga ctatcccgat tggacgaact ttcaacatca gtaaagatac attaacagtt
    11761 aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc tgatcctatt
    11821 gaaaatacgg agcaagagaa caaaaaatcc gagactacaa aagaatcgga accaacagca
    11881 aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag ttcatctaat
    11941 acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag tagcactacg
    12001 gaagctgata ctggaacatc aacaaataac tcacaatcaa catcaaatga tgcaaatgaa
    12061 tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga aactgacagt
    12121 tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt tccatttatt
    12181 agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt acattataaa
    12241 ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact atctcctgac
    12301 aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg aacttatcaa
    12361 ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa tgttgtagat
    12421 atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa tcctgtagta
    12481 gcgtgggtaa cttatgcctt tgaacctgct caacataaac caatgttttg gggcgatgct
    12541 atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta ccatgttgtc
    12601 gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc atataacgta
    12661 tctcaattag cggtttcggt acgttaataa aagatatcgt ttatatacta agtagttata
    12721 tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac
    12781 taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaaa
    12841 tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga
    12901 catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag
    12961 catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg
    13021 atgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa
    13081 ccgacaagtg caaaatttat agaaactggc gtaaaaacag acggttatat tagggttaat
    13141 atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat
    13201 attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg
    13261 aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca
    13321 aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta
    13381 gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac
    13441 cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct
    13501 aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac
    13561 gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag
    13621 ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa
    13681 attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca
    13741 ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact
    13801 tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag
    13861 gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa
    13921 gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat
    13981 gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc
    14041 ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag
    14101 gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt
    14161 aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct
    14221 gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga
    14281 cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca
    14341 actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact
    14401 tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat
    14461 caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca
    14521 caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg
    14581 tatagcctag aagaaaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt
    14641 atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa
    14701 ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc
    14761 accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct
    14821 acgttcaaca ttattgcgaa caaaacttat aacttagaag aaacaaaagt accaacaggt
    14881 tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa
    14941 tatactgcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt ggatgatcaa
    15001 gggaaagcat tagcaaacgc agaattcact ttgacagacg acaaaggtgt aaaagttgta
    15061 caaaaaacgg acagcaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa
    15121 atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc
    15181 gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca
    15241 gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg
    15301 agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga
    15361 aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat
    15421 ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc
    15481 cttggactag gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga
    15541 acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt
    15601 gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag
    15661 ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat
    15721 actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac
    15781 agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta
    15841 aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt
    15901 gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat
    15961 caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca
    16021 gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga
    16081 aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta
    16141 actgtggatg gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat
    16201 gctaatagaa caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg
    16261 gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta
    16321 attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt
    16381 cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta
    16441 acttatgatt catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa
    16501 cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa
    16561 caatttgatg caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa
    16621 gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa
    16681 cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt
    16741 aaaacagacg gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa
    16801 attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaagc tgttacttgg
    16861 attgcagata aatctaaagc tactgaattc gtttccggat cagacggtaa gttccaaatt
    16921 gaaggtttgc aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca
    16981 gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca
    17041 actagaaacg atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac
    17101 atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt
    17161 ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac
    17221 aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa
    17281 gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa
    17341 ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct
    17401 actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa
    17461 aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag
    17521 aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc
    17581 agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt
    17641 tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg
    17701 gaactgatta tccaactggt gggattggaa accattctgt tatatcagct cattcaggca
    17761 ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta
    17821 ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa
    17881 ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta
    17941 cacctgttgg agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa
    18001 aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact
    18061 ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa
    18121 aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact
    18181 cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa
    18241 acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa
    18301 gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac
    18361 aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt
    18421 gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat
    18481 caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca
    18541 aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt
    18601 gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata
    18661 gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc
    18721 ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca
    18781 gacgttatta gaaattacgg tcagaattca acactgactg atattcctga tttagatatg
    18841 ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt
    18901 ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac
    18961 gctgatgcca ttgatagtga atccgtttca gataagccag aaactgttac atcttctaat
    19021 gatttatttg aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat
    19081 attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca
    19141 atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtatta
    19201 ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttag aaaagcataa
    19261 tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct
    19321 tttttatttt gaatagagct agattcactt ttaaataaaa taaaaactga tcttaattcc
    19381 aaagaaaaag tcaagaaaaa aatacaacaa atttttttat cagaatattt atcagaatat
    19441 tattagaaaa gataaactca aaagcttaat ttaatagggt tattggttgt atttagaaat
    19501 aatgtatgga aaagctataa agcaatagat aagatgtaga aaatcttatt tgtgaatgaa
    19561 aaacaagtca tcgtaaaata ttaataggag gtgtagtaat ggtggaaata tcaaaaacag
    19621 ctttagactc attagctaaa ctatttagta gcaggtttga tggtgcagaa tttgttgaat
    19681 atctaagcca aaacgaaagt ggaaaaactc tgatcgaaac actgaaaaaa gactatttga
    19741 atgaaaatga ctttccgaca tggacgaata ttgaagatgc tacatgggat gtagtgtgta
    19801 cgatatattc tccttgggta acgattgctg attttgtttt agataaaatt ggaggggata
    19861 cataccctga tgccaaatct cttgctacaa gtgactttaa aacgttctat ggttatctaa
    19921 tgggtctaat aggcaatgga atagatttga aagttacgcc aaaacaaaca cctgaagaaa
    19981 ttgaaaaaga aaaaggagag gttcaaatgg cttatgcaga tgacagagaa aaagtaaaat
    20041 tattggaagg taaattatct gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag
    20101 acagcaacta ttttcctgaa ttgaaggtat atcaaaacga cgaagatttc tttaatgaaa
    20161 tgtttagtaa tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa tggaactttc
    20221 aagatgatct agttgcattt agcaatgatg ggtatttacg atcatattca gaaagtgagt
    20281 atagagctga attaaaaacg tatgaaacgg atattctgat gagctattta aatctattag
    20341 agagtggtca gggagagtta agagataaag ttgaagaact attaaaagaa gagctatagg
    20401 gattatttca acataaacac aagcagaaaa ctaccaaaca aaacgtacca tgcaaaaagc
    20461 cggttaaacc ggctttttgc tctattatga gaggagatca aaccatgagt gcaacaaccg
    20521 atatggcttt agaagcagta ataaaagata atacaggaaa tgtcgaaaat gtagaagact
    20581 ttagcgatat tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg
    20641 aatttgagat caagaatatg gatgacttaa aagcaaaatt tccttttatc gcatcgaaac
    20701 taagtcaatt tgccgaggag aaatcgtgga atgaattagt tagtttttcc gaaaaaattt
    20761 atgatattac gcaagaagat gagtgtgttt catgggaaat gaataatcaa tacaagaaaa
    20821 ttgatgcttt cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa ggtatagata
    20881 ccgcagataa ttggttaaat aaagaatcct ttattaaaag tatagcggat gatcttgaaa
    20941 aacaatggtc taaggaagac tatgcagagt acctaaaaac agaaagagat acagaggtga
    21001 aagaaaaact cactgaggta tgtttggatg aacatattga tcccaatatc gtaaaacaag
    21061 aagactatga agtggttatt gataaagttc ctactgattt tgaggaagtt gtaaagtata
    21121 tagttgaaga agcattatcg caatactctt cagatgagga agtggacgaa gaaactgttc
    21181 gtaccagagt ttggcaactg gtttttgatg acaaagatta taaatatttg agtgatcaaa
    21241 actcggttaa ttggagtgta cgattattaa ttgatcaaaa tgatattgaa taaaatagag
    21301 ctgaaaagct ctattttttt tgataaaaaa actagctatt tttttaaaaa taatttggtt
    21361 caaaatttat ttttttaact tgataatttt ttgccatcct tttttaaatt ggctatatat
    21421 caaggtttat agtggggata aataaaaata catttgacaa aatttacaat atagtttatt
    21481 atatatttat cttattatga taacacaata aaaggaatga tggtatgaaa aagaagaagt
    21541 ttagtcgatc gttgttagga attttagttt tagttgcggt tggtcttagt ggcaagggtt
    21601 actttcagaa taacagcact caggaacatg attttcagat caaaagtagt gttcaggtag
    21661 gtaataaagc agacagtgaa tcaaaagaat ttttagggat acgttatgat ccaaacaaat
    21721 atccaaataa tatcgtaaac attaatgatg ggaaatatac actgacaaaa gaagaccatg
    21781 aagcattatc agaaaaaggc agtcaaacct tttgggtaaa ttatgcagat ttagatcaac
    21841 ttggaagagc aggagatgtt gaagcgttga tcacctatga tgctataaat tctcactcat
    21901 ctaaaaatgt ggaacgtcca tctttcaaaa gttctgtcca tattgcagga gaatacaagg
    21961 atggagtatt tgatcaagag agagatactt gggtcggcaa agattcaaac aatgaaatca
    22021 ctcagctaga aggatataaa ggttatctct ataacaaaag tcattcatta gcatggtcgc
    22081 taggaggaga tatggaggta cacaatttaa cacttggaac gagagcgcaa aatgttggga
    22141 aaaatgatca gaatggaggg atggcttact cagaaacgat tgtacgtgat gcaatctata
    22201 aaaataagaa tgaaaaagta ttgtataaag ttgatccaat ttataaaggg aatgaattgg
    22261 ttcctcgtgg ttctcatgtc gaagctttct cagttaacga taatggaaaa acggtaaacc
    22321 ttaatgttta tgtatttaac aatcaaaaag gaatcaacat caactatcaa aatggtactt
    22381 gggtaagaga ttagaaattg agaaggtgaa gaattggaaa caataagcat caatggatta
    22441 gataaatcag ttgcaaatcg aataaaaaaa ggaaatggct actatcttct gaagaagggt
    22501 aaatattcag ttgctagaga gttagaaaca atgagtatcg tgtatgtagc taagaatcat
    22561 tttgacgtag aacaggctat ttttgatcca gattctgaaa tagggaaatg tgttgaatta
    22621 tcagagaaaa ctgttagtac aaaaggggcg atgatcattg agagagaaat gttagacgaa
    22681 atcagagtaa tcattcctaa agatacgcag atgtgtgagg acagcataag tgacgatgaa
    22741 ccaaaatcag aaagaaaata aggagatatt cagatgagcg aaaaatccat tgaattaaag
    22801 gaatataagc gaagcgatgg agaaaaatat tatcctgtag ccagtttttc taaatcagta
    22861 tatctggttg gacgtgcatt ggtagttggt gcaattggca tatatatttt gcattgggta
    22921 acaaaggttg tacaactgct tgtagatacc ttagtaagta ttcttgtctc gttaaatgat
    22981 gttaacggaa cagatgattt aggattaaaa gaaatgttta aagagttact caatgcgttt
    23041 tttcccaatt atttaagtta ttcatataat gtacaaagct tttttgcaaa gctacacttg
    23101 gtagacatct ctattttagt attagaagca ggaacggtat tctttgttgg atatttggtt
    23161 actttgatgt tcaggcatca ctatggagaa caaagaccat tttttgatga tatggaaagt
    23221 agaaggttga aaagagaagc tctagaagct atggatgtga agtatgatcc aaacaagatt
    23281 ataggagatg aaacgaacaa caaaaaaaga aaacgaaaca aaacgcaatt agaatccgat
    23341 gtgctatcaa cgcttaaaaa tatcaaaatt tctgttcata caagacgtga tctaagtgtc
    23401 aatgaagata ttcgagaata tatcgtaaaa atcccgcaac caaaagggaa gaaagttaga
    23461 gaaacagttt tacgacaaat tcaagattta gggtcgatcc tgacaagagc aacaagcgga
    23521 cgagccaaat ttggtgaaaa agaagatgaa gtttcaggcg attatttcat tttccgagca
    23581 tcagtaaata ttgaaagaga acgtaaggaa agagtaagta ccaaaaacaa agaaacggaa
    23641 agagcaacat cttcaatcgc agtttcaaat tcacaatttt cttttccaat caatctcttt
    23701 gccgataatt ccaaaaatat ccaaaaccaa accgaaaaag ctagaaaata tgccgaaact
    23761 caaaagcaat ctttagaact ttatctatct tctgctgacg tacctgcaag tctaacagat
    23821 atgcacgttg ggaatacatc tattgaatat ttgtttactt tatctcgtac cagaaaaatt
    23881 caactaagct caatgaaaga agaaattgaa acttacttgc atatatcagg tgtaatcgtg
    23941 cggatcaacg cagggcgtat tcaaattact ctaccattag gagaaaatga tcgtattcca
    24001 attgatgtac catcaatgat caatagcgtg ttttaaaaga catcacaaat agaaacagaa
    24061 aatacaaact accagttcac aaacagaaaa gtaccgagaa aagtgaggaa tagaaatggc
    24121 tatgaaatta ttagagaaag tcaaaaagag agccgaaaaa gctgaatcta ttttgctaga
    24181 taaagaacct aatataaagc ttaatggaaa attcagtgtc aatatgttga acacaaaggc
    24241 taaagacccg acagatgcta tttttggaat taagcctgat ggcacaattg ctcatttttg
    24301 catcagcgaa atgccacaca tgttggttgc aggaacgaca ggatcaggga aatcagtatt
    24361 actgaatgaa attcttgtta cagcaatgtg tcactcaacg cctgatgaat taaaaatagg
    24421 aattgtagac ccaaaaaggg ttgaatttgg aagatacaaa aaattgccgt tcatgttggc
    24481 tgatccaatt acagatatgg atgaagctta tgattttttt gaatatcttg tgatcttgat
    24541 gcatgaacgt tacaaactga tggaaaaagc agggttacaa aacatcacgc tatataacga
    24601 atatgccgaa aaaaatggat tagatagatt tccctatgtg atcttattag ttgatgaata
    24661 ttcacagttg gttggaacac ataaagaagt agaaggtttg attgttcaat taggacaaat
    24721 ggcaagggca gcaggtattc atgttatctt agctacacaa tctcctcgat caacagttgt
    24781 cactggtatt atcaaagcca attttccgtc acgtgtttgc cttatggtag caagtgattt
    24841 agaaagtagg atcgtattag atgaaggcgg tggagaaagc ctaagtccaa agggagatat
    24901 gattattaag ctcgttaatg gaagtatggt tagagcgcag ggagcttaca tcagtaacaa
    24961 agaaattgaa acaattttca atcaccttag aaatactatg ccagaaccag aatatgttga
    25021 ttaccgagca atcgtagctg aatctcgtgg tgagggtgga gaagacgagg attttggtgg
    25081 aattggtaaa gctcctgtaa gaaaatctgc taaaagaaat ttgaacacac ctttagcaaa
    25141 ttcatcatct aaagagccat caattgaaaa gaagagtacg tttgctctaa aacttgaaaa
    25201 aaacaaagaa gaaaaagaaa aacaaggagc taaaacacta gaaaaacttc aagccgaaaa
    25261 attagaaaaa gttttaaaag cattagataa agctgattct gaaccgcaaa cctcaaacaa
    25321 cacgaataat gaacagccga tttcatctaa aactaaaccg aaaactactg atccgaaagt
    25381 ttcagtaaac gtgaccatca caaccgataa gtctgagaag aaacaagtaa acggaacgac
    25441 ttcacctatc atgcaaaaaa tgttgaaagc caaaaaacag gtgcataaat gatcattgtc
    25501 agttcttttt tgaggtggct tacgccatat aaatatacag atgaagtagt tgtgaaacga
    25561 acgaagaaaa gagataaaaa agcggtacta actcctaaac agatgcttga tcatggcatt
    25621 ttaggggtga aggttttaga agtgaataaa ccgtttgaat caaatagtgc aagagaaaag
    25681 ttgcttggag ctgaagccga tgtcgcatat ttttaccatg acaaaagcaa gcatcaaata
    25741 gctattaaat tcaatccaaa atcggcatgg cggtatcaag caagagatga cttgaaacgt
    25801 aggaaactta gaccaatgct ctatccagaa ggaaaagaag catttgatag aacccacatc
    25861 gttcccattg gttttcatgg tacagaatca gataaccgcc tattagtagg ttggaacagc
    25921 aatcaaaata gagttcagat caataaattt gagaagaaag taaggaaatt caacgatcga
    25981 aaaacgatcc tatggtttgc agatatttgt aaacagccgg atggaacagc aaagtgggaa
    26041 gcggtgatat ttgacgaaaa tggtaaaaaa gtaatgtctg aaacatggca tgataaagct
    26101 aaatttgttt ggcagtagca gtagaggtga aaaagattgc tagaactttt tgaagcaaat
    26161 agaacaggct ttaatcaaaa gttagaagtg ggagaaacta tagaatataa agataaaaac
    26221 tatattatta tcggaatcta taacacgaga attaacagaa taggtgatcc acgtataacc
    26281 agtgacttgg tttgtcaatc tgttaactat tcacctgatc tgacaagcag gtataagaaa
    26341 tacgtgttat tagaatatag acacaagatt actgatgaaa ttggtgtaaa caacacacga
    26401 aacatattta agatcggaac agtgattcct tatagtaaca acgaagaaaa aatattttat
    26461 cagatttacg gaatagaaaa gtttgaatat gaacatgtcg atctgctagt gacgtaccaa
    26521 atgcgtttaa tagaaccttg atcacaggca gaaattgata aagcagtaaa attgaaccga
    26581 ttatcaaaat ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa
    26641 ataccaaaaa attagcaatg aacgtaggta agaacatgac aataaataat aataacccta
    26701 aaaaatataa tgctcttgtc caatgctata tcgaaaacaa aattacaatg caagaagtcg
    26761 ctgatgaatt aggcaaatca aagagcctag tatcacacga ttttaagcac aatgtttttc
    26821 cgaacccgat tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa ataggtaagt
    26881 ggattgatga agggaatgca attgaccctt taattgaaaa atatcctttt ttaaaaagca
    26941 aaaataaaat tcaaaggtta attcgtcaag gcgatattca agctaaagaa cctgttttga
    27001 caaccaatga tttggctaaa ataaatcttc ataaacaaat tattgaagac tttgaaaaaa
    27061 atctttcaac aactgaaatt ttaaaaaaat acaatattcc gagaggaaag ctaacatcga
    27121 ctctttttag agaatatgga gaatcggcag ttactaaagc aaaggaagta gcgaatgaga
    27181 gaaaaaaaca aaaatagtag tcgataccta gtagcgactg tagtttttgt ttgtgtattg
    27241 attttcctgt ttgatttttt gcagattgga aaaagcataa ttggaaatac attgagtgca
    27301 aaggaaatgg ttgctttagg tggtttgtat gaaggacact tgttcccgaa tatattcatt
    27361 tctatcgttt ctcatattaa cttgtaccat ttaatttcta atttattgtt cttagtagtt
    27421 cttgaagaag cactgaatga tcagtatgag tggttaacat tattgatcgt tttaatcata
    27481 tcaggatttc taggaagctt agttcctttt ctgctacaca gcaacatcgt aagtatgggg
    27541 cttagtggag gtatttttgg atggattgct ttatttgcga ttgcatacat ccgttttgat
    27601 agcaagaaac atttattact aagtgttttt gtattattga taatcggcat tttactggta
    27661 tctacttttg aatcaggcga gacaaacgtc ttgtcacatg taatcggatt tgtctgtggg
    27721 atgatcttgg gatttattct aaagaacgtt tcattgatca aacaaaagaa ttaaaaggga
    27781 gtgctaaaaa tgccaaaaaa aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa
    27841 atgttatcac ttcaaatact gttaagaaaa aagattgaaa caatagacga agaagttaga
    27901 aataaaaggg ctatgtacga acaacgtgaa gcgctaagag aacgaatcca ctattttgat
    27961 gatatacaca ctaaaatcta tgaagcacgt acaaatcacg aagacgaaag caaattagga
    28021 ttagctcaac aacgaatatt tgaatggctg aatacgaata ctgagcaaga accgttaaga
    28081 gcaattagta atctttttga atttggaacg aagcattggt ttaacgctgt agaaaaggat
    28141 gctgacttac aagaagcgtt cgatacacta agcgaggata gcaaatccat gcgagactta
    28201 ttgatcactt atctaaatga gcatgaattt gaacagacga aagttaaaaa gaaaaataaa
    28261 aaatagaaag ttgtgatgta tcatgctgtt taccttatta ggttatttat gtttagttct
    28321 tgcttttttt ggagcaattg ctatatttgt cggtcagttt caagaaaata atccgataat
    28381 gcgtaacata ggcattggaa ttgttatcat atccttgatt gtatttggtg ccacaaagat
    28441 ttttgggagt aatgatcagc agtttggtat ttctggagtt gataatagtt atgctccaga
    28501 ttcattggga agagtagtga tagatggaac gtttgagaat gtaacgagct acaaaaatgt
    28561 ttcattgaca gtgaaagaag atgacaagaa agcatcagat gacgttctcg ctacgatcaa
    28621 gccgtctcaa tcacagtttg aagtgtttta tacagtcaaa gagaataacc ctgtacgcaa
    28681 gctaacatta gtttttaacg ttgacggaga agaatactat aaagatgtaa aaatcaataa
    28741 agcatcaacg aaaaagattg tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac
    28801 aactgaatca agttctactg tcgaagttga actaccagat aaaattgatt ttgatcctga
    28861 aaaagtcaaa tcgtttgaat ctgtttctgg ttctatggca gatcggttta acactgaagt
    28921 tttcaaagct atcaaaactg ttaaattgga tactgaaaag caaaggatta atgttacctt
    28981 attcaatgca gttgaatcta tggatcagga taaaaaagtt acggttgcaa gagaactagg
    29041 gaaaagagta attggattgt atcgaggtac ggcaaaacca gattcagacg gttcagattt
    29101 gccagttcac gtagtttatg aagacggtaa agtttttgga gactctacag caaatgaagc
    29161 actaacgatt aaattagcta aatagccgga aaattataat aaacaagtgg tgtcacacca
    29221 ccatcgcata ggtgtgacac cttggagatg attactgatg gtagtaaata aagaaaaagt
    29281 aagaaagtat gttactttaa aaatagcttc tgttgaatat ttagaaaagc gagctaaaga
    29341 agaagaaaaa acaagaggta agaaggtaac agtttcagag ttgttagaag aattaattga
    29401 gagtgaccaa acaataagac gtgcatttac caataggtct gttaaaagtt agggtgatac
    29461 tatctttaat ttcaaccaaa tttctaatat atgatgaata gtttccttgt acaaaatatc
    29521 taattaagag ataaaagtta ggaggattca ttcaaaatga ttatccaaaa gtttgactgg
    29581 aaaatgaaat taggaattag tcttgtaata ttgggattta ttattttttt ggggataaaa
    29641 gcacaaaaaa atcaatctac ttttgaaact atgtcttctc aaattattga ggaatgttcc
    29701 aaaatttatt attgtgagaa caaaactgaa gaatctaatg gcttttatta tatcgatcaa
    29761 ccacatgatg ttgttataaa aatggagtca catgaagtaa acgaatggat tcaaagaagt
    29821 actaatgtat caaaaaagtt agaaagatat tctcaaacgt ttttaagtaa agtcattttt
    29881 tcaaattgca aaaatgaaga aacgtctcaa agagtagcta gttctttaga aaatttaaaa
    29941 ttttctgttt caaaaaaaga gaaatttgta gatttacgaa aataaactat cgaaagtagt
    30001 atgattatga tcttaaaata tcatcttaag agaagaatta cgattgttta taggatttac
    30061 aaaatgaata agtttgaaga gtgatttggt cgcagactag atcactcttt ttgtgtaaaa
    30121 aaactttaca cttatttttg agtgtgttaa gattttttat atagagtgac ggtttcttgt
    30181 cagagtggat gataaccatc aatattggag gaataataag tggaaaacaa taatgatatt
    30241 agatatttta gcattgggga aagagttaca acagtaagtg aattatcaaa ggattataga
    30301 gataaagaag gaacgattat ttctcttaaa aagaatcgaa gaggtgtttg gcaatatgtt
    30361 gttgatttag gggatggcgt tacagttgag tgtaaagata gtcaactgat cagaaatgat
    30421 ggcagtcatt ccattgaaaa agcaattctt gtattcccaa aagtatcaag caagattatg
    30481 aatgactggt tgcttggaaa atttggtgag tacaataaaa aagaaatcgc agaagtgatc
    30541 ggcaaatctt atccaacagt attagggtat ctaaaaaaga taacgaacaa agaagcgaaa
    30601 gttgaggatg ttttaacagt aagagaactt cagtcattaa tcgaatatcg gaataaacaa
    30661 ataacaaatc ctgagtacct taaaaaagaa gatggtctat tgaaacaatt ggaacaggta
    30721 aataaaatcc ggaaaatgca aaaggtagct cctttaagcc tagataactt taaaaaaatc
    30781 tatttgaaag agatcgaaaa agactaatgt ggagtgtcac tttaccgttt caacctgtat
    30841 ctgcaccaag accaagtgca agaaggaatg ttgctgatgc aactaatgac gtagcagaga
    30901 aaaggatttc aacctattat gataaaaagt atctcgatta tttgaaaaaa ataaaaagct
    30961 ttatcgaaga tcaaggtttg ttagatgatg aattttactc gatcgtaaat gatccgatgg
    31021 gtgcaattat ggaagtagac ttttactatc agataccaaa aagctataaa aaagtctata
    31081 acattatgaa aacgacagcg ccagatttag ataatttagt aaaaagtgct atggatggta
    31141 ttttcaacat ctcagctata cgagatagcc atatcacagg gttaattgct tttaaataca
    31201 atgtggcaaa cgaaggaaaa acagttgtga ggatcaagcg atattcagaa tttgagtggg
    31261 atacttctga aggattgaac ggagatattt gggaagccac atttgatttc aaaccagtag
    31321 caacgccaag accaaagtct aaatttagag gaaatgggat tatcacttac tatccaaaag
    31381 agtataacga ctatttggaa aatatcaaga atacgcttaa agagaaagat ttggtaaatg
    31441 atcaactgaa aaaggtaatg aatgctccta tgggggttat agctcagata gattattttt
    31501 accaagtgag aaaagataag aaaaagcttg attcactgat gcgtacttca cagccggata
    31561 ttgataactt agtcaaagga acattggacg gaatttttaa caaggaaatt ggcatcaaag
    31621 atagcagggt ggtaggttta atagctttta aatacaatac ttttgaaaag tcgaaaacaa
    31681 acgtgcgact acaggagatg ggctgatgaa taataacgaa aaaattcttc atgtacttga
    31741 tagctttgaa acaattcaag aggaactaaa gaaatatcga gatgttttgg aacaaagata
    31801 tgattttgtc aatcagcaaa agagtaatca catggatttc attctcaata tgaatgactt
    31861 gaagaagaaa ttagttgaac gaaaagaaca agaaaagttg atcaaagctt actttgaact
    31921 cggtgaaaaa gaagttaaaa aggcaatgga actgaatgaa gatcgcaggg ttttagatca
    31981 gctgttagaa caattattgg tgatgtttca aaaaggacga atagatgagg atttaattga
    32041 agaagggttg agaaagtatc ctgcaaactc aggtatcgga atagtgctga aagcgattga
    32101 tgaagaagaa attgaagatt tcataccggc agaggatttt gaatcggcaa tggaatatat
    32161 aaaatattac agccaaggga tcactgcttt tagagagttt gatcctgaag atgttattca
    32221 tgacttgaac aacctaaaag aatggtgtga aggttatgaa gttgatgatt ccggtttgga
    32281 ctacttgatc agtattatgg aaatcgaaga agagatgccg gataagcctg atccaacaga
    32341 tattttagaa ttaatccatg atgctagaaa tccaattgca tacatttcta gaggatatac
    32401 tgtattggaa tactataagc cttatatatc agcgatgaac catttaagac gtgtattaag
    32461 ggaaaaacgt gaataccgct cagtgttgaa tgcaacgaat agattagaaa aagctgtttc
    32521 agagctagat gcttattatc gtgaacatta cctgcaagca ggtggtatgc caagaaatac
    32581 gaaagcaaat atcagtaggt atataaaaaa agctgagtag gcggagttag cccataaaat
    32641 ggcaaaaggt aacctgaaag gggcattttg aatgaaaagg gatgtttttg aattgtacgt
    32701 attagaacgt ctagaagacg gcatgatggc acaagagata attgatgaaa ttgatgatat
    32761 gatcagcaga tttggtcaat tggagttgaa tgatggaaac aaaaattcgg tagtgctaaa
    32821 acaattttgg ttgaacaatt tttcgtataa ggattttatc aaagaaaagg caaatacatt
    32881 gtactgacta aattcacgag gagcagtgat gagagcttat tgctaagagc agttaggctc
    32941 ttttttgttt gtgtaaaatt atttgtttta taatacacat ttatacacaa aaaatattga
    33001 cagatagtac acaaatgtgg taggatatat ttgtaagcaa gttagattaa caagttaaag
    33061 gaagagcagt tatgaaaaaa gaagaactaa tagatatatt tcaaattgtt gaacgagcaa
    33121 ataatatggg aattatgttt tttgatcgta tttctttaaa
//
LOCUS       DAPBCS010000028        30976 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPBCS010000028 DAPBCS010000000
VERSION     DAPBCS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.028"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..171
                     /locus_tag="RD521_001599"
     CDS             <1..171
                     /locus_tag="RD521_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7771336.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="RD521_001600"
     CDS             406..642
                     /locus_tag="RD521_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771337.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="RD521_001601"
     CDS             649..2601
                     /locus_tag="RD521_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDT7771338.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="RD521_001602"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="RD521_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDT7771339.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3532..4572
                     /locus_tag="RD521_001603"
     CDS             3532..4572
                     /locus_tag="RD521_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7771340.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4670..4858
                     /locus_tag="RD521_001604"
     CDS             4670..4858
                     /locus_tag="RD521_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDT7771341.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4916..5269)
                     /locus_tag="RD521_001605"
     CDS             complement(4916..5269)
                     /locus_tag="RD521_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771342.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5800..6222
                     /locus_tag="RD521_001606"
     CDS             5800..6222
                     /locus_tag="RD521_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7771343.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6219..6449
                     /locus_tag="RD521_001607"
     CDS             6219..6449
                     /locus_tag="RD521_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771344.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6947..7147
                     /locus_tag="RD521_001608"
     CDS             6947..7147
                     /locus_tag="RD521_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7771345.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7174..8367
                     /locus_tag="RD521_001609"
     CDS             7174..8367
                     /locus_tag="RD521_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7771346.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="RD521_001610"
     CDS             complement(8435..10000)
                     /gene="guaA"
                     /locus_tag="RD521_001610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDT7771347.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10059..10982)
                     /locus_tag="RD521_001611"
     CDS             complement(10059..10982)
                     /locus_tag="RD521_001611"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDT7771348.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11178..11783
                     /locus_tag="RD521_001612"
     CDS             11178..11783
                     /locus_tag="RD521_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7771349.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12091..13107
                     /locus_tag="RD521_001613"
     CDS             12091..13107
                     /locus_tag="RD521_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7771350.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13248..14549
                     /locus_tag="RD521_001614"
     CDS             13248..14549
                     /locus_tag="RD521_001614"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDT7771351.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14595..15257
                     /gene="deoC"
                     /locus_tag="RD521_001615"
     CDS             14595..15257
                     /gene="deoC"
                     /locus_tag="RD521_001615"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDT7771352.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15278..15676
                     /locus_tag="RD521_001616"
     CDS             15278..15676
                     /locus_tag="RD521_001616"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDT7771353.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15742..16824
                     /locus_tag="RD521_001617"
     CDS             15742..16824
                     /locus_tag="RD521_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7771354.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17036..18598
                     /locus_tag="RD521_001618"
     CDS             17036..18598
                     /locus_tag="RD521_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771355.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18591..19724
                     /locus_tag="RD521_001619"
     CDS             18591..19724
                     /locus_tag="RD521_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771356.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19724..20680
                     /locus_tag="RD521_001620"
     CDS             19724..20680
                     /locus_tag="RD521_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771357.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20779..21945
                     /gene="deoB"
                     /locus_tag="RD521_001621"
     CDS             20779..21945
                     /gene="deoB"
                     /locus_tag="RD521_001621"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDT7771358.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21969..22787
                     /locus_tag="RD521_001622"
     CDS             21969..22787
                     /locus_tag="RD521_001622"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7771359.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22804..23511
                     /gene="deoD"
                     /locus_tag="RD521_001623"
     CDS             22804..23511
                     /gene="deoD"
                     /locus_tag="RD521_001623"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7771360.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="RD521_001624"
     CDS             complement(23809..24495)
                     /gene="gpmA"
                     /locus_tag="RD521_001624"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDT7771361.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24667..25248)
                     /locus_tag="RD521_001625"
     CDS             complement(24667..25248)
                     /locus_tag="RD521_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7771362.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="RD521_001626"
     CDS             complement(25381..26064)
                     /gene="rpiA"
                     /locus_tag="RD521_001626"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDT7771363.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26383..26796
                     /gene="rpsL"
                     /locus_tag="RD521_001627"
     CDS             26383..26796
                     /gene="rpsL"
                     /locus_tag="RD521_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDT7771364.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26880..27350
                     /gene="rpsG"
                     /locus_tag="RD521_001628"
     CDS             26880..27350
                     /gene="rpsG"
                     /locus_tag="RD521_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDT7771365.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27444..29531
                     /gene="fusA"
                     /locus_tag="RD521_001629"
     CDS             27444..29531
                     /gene="fusA"
                     /locus_tag="RD521_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7771366.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29703..30890
                     /gene="tuf"
                     /locus_tag="RD521_001630"
     CDS             29703..30890
                     /gene="tuf"
                     /locus_tag="RD521_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDT7771367.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         9895 a         5625 c         6425 g         9031 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tattacgtgg attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg
     3841 gaagtgatta gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct
     3901 acagtcattt atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg
     3961 ccgccaaatc ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt
     4021 ggaatgcagt atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca
     4081 gttatggaag ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat
     4141 tgtaaaatct gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt
     4201 cggatgctta ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta
     4261 gagccatatc ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac
     4321 gatgctccca aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt
     4381 attattggag aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt
     4441 acaaatgggc ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa
     4501 cctgtttgcc agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat
     4561 aaaataactt agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga
     4621 aatattcttt aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta
     4681 caagtggata ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac
     4741 tatacttaaa aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt
     4801 tcaaaaaatg aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt
     4861 tgagaaatga aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc
     4921 cttcaatatt tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt
     4981 tgtctgtaaa gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat
     5041 tatctgtagg taagaaaaat tcatcaatag atacatggag taatgataca agatcataca
     5101 gcacttgtgt acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa
     5161 tttcaatcat tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa
     5221 tcgctaaacc aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc
     5281 tctatacatt ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag
     5341 tttatgagtt cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata
     5401 gaaaggtcgc tgattttatt tacctataca actttataaa atttagtcat actcctagaa
     5461 agtagataaa attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca
     5521 tctagttctc ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat
     5581 gaccatcaat gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga
     5641 aaacaatctg tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc
     5701 atttatgcaa caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca
     5761 catgccactc cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa
     5821 gaaaccatag aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa
     5881 gattacttca aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat
     5941 aactaccttg tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt
     6001 aaattatttg gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat
     6061 ctttcagaga aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg
     6121 gagattgcgg agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga
     6181 ctagccatga ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg
     6241 ttcccttccc cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg
     6301 ttaaacatta tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg
     6361 gaaattctca tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct
     6421 ctaagatttt atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg
     6481 tatccacgct tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt
     6541 aggttttgat aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata
     6601 cgtttcggta agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg
     6661 attcatgtta gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg
     6721 tgataatgat actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt
     6781 acggctacat gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga
     6841 ttgctaagat attcttctct tgtcagtgta ttattataaa actatttgat ataattatag
     6901 gtgaatattg gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac
     6961 aaaaaaggat ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa
     7021 agcaaaagaa ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag
     7081 agtccctcgt gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc
     7141 tgagtagttt ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca
     7201 aagaatggaa cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt
     7261 caaaaatatc gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg
     7321 ctattgatga aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat
     7381 atctttgaaa ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat
     7441 cgcttaccaa ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt
     7501 gagcctattg atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat
     7561 gtaagagctg ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata
     7621 accaccacaa atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa
     7681 ttttggacaa aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat
     7741 caacactttt tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa
     7801 gctactgcca ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa
     7861 acactctatt acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc
     7921 gttcgtcata ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta
     7981 caacaaaatc taattcagac agactttgta atgagctata acggtattcc tacccaaaaa
     8041 catacactat catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga
     8101 atccatgggt tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt
     8161 atcattaaag atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat
     8221 ctttatccta acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa
     8281 gaagcaaaac aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga
     8341 aacaaagaaa tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac
     8401 ccttggtata agaggatttc tagctctcct acaactattc ccactccact gttgctggtg
     8461 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
     8521 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
     8581 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
     8641 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
     8701 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
     8761 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
     8821 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
     8881 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
     8941 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
     9001 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
     9061 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
     9121 acttgtctaa gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc
     9181 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
     9241 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
     9301 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
     9361 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
     9421 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
     9481 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
     9541 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
     9601 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
     9661 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
     9721 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
     9781 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
     9841 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
     9901 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
     9961 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    10021 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    10081 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    10141 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    10201 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    10261 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    10321 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    10381 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    10441 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    10501 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    10561 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    10621 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    10681 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    10741 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    10801 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    10861 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    10921 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    10981 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    11041 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    11101 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    11161 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    11221 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    11281 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    11341 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    11401 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    11461 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    11521 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    11581 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    11641 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    11701 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    11761 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    11821 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    11881 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    11941 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    12001 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    12061 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    12121 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    12181 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    12241 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    12301 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    12361 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    12421 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    12481 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    12541 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    12601 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    12661 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    12721 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    12781 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    12841 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    12901 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    12961 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    13021 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    13081 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    13141 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    13201 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    13261 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    13321 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    13381 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    13441 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    13501 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    13561 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    13621 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    13681 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    13741 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    13801 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    13861 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    13921 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    13981 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    14041 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    14101 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    14161 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    14221 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    14281 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    14341 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    14401 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    14461 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    14521 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    14581 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    14641 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    14701 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    14761 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    14821 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    14881 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    14941 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    15001 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    15061 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    15121 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    15181 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    15241 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    15301 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    15361 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    15421 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    15481 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    15541 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    15601 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    15661 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    15721 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    15781 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    15841 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    15901 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    15961 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    16021 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    16081 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    16141 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    16201 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    16261 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    16321 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    16381 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    16441 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    16501 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    16561 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    16621 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    16681 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    16741 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    16801 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    16861 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    16921 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    16981 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    17041 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    17101 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    17161 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    17221 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    17281 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    17341 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    17401 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    17461 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    17521 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    17581 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    17641 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    17701 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    17761 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    17821 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    17881 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    17941 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    18001 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    18061 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    18121 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    18181 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    18241 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    18301 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    18361 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    18421 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    18481 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    18541 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    18601 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    18661 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    18721 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    18781 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    18841 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    18901 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    18961 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    19021 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    19081 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    19141 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    19201 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    19261 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    19321 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    19381 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    19441 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    19501 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    19561 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    19621 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    19681 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    19741 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    19801 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    19861 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    19921 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    19981 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    20041 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    20101 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    20161 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    20221 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    20281 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    20341 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    20401 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    20461 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    20521 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    20581 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    20641 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    20701 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    20761 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    20821 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    20881 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    20941 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    21001 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    21061 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    21121 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    21181 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    21241 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    21301 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    21361 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    21421 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    21481 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    21541 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    21601 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    21661 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    21721 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    21781 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    21841 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    21901 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    21961 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    22021 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    22081 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    22141 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    22201 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    22261 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    22321 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    22381 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    22441 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    22501 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    22561 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    22621 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    22681 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    22741 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    22801 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    22861 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    22921 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    22981 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    23041 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    23101 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    23161 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    23221 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    23281 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    23341 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    23401 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    23461 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    23521 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    23581 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    23641 tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg
    23701 tttgatgaat attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa
    23761 aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta
    23821 ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat
    23881 ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa
    23941 tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc
    24001 atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat
    24061 atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc
    24121 ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc
    24181 gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt
    24241 taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt
    24301 acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc
    24361 ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac
    24421 atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa
    24481 aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca
    24541 gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
    24601 tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt
    24661 taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca
    24721 tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata
    24781 atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt
    24841 tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc
    24901 catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat
    24961 tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt
    25021 taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg
    25081 ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc
    25141 atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt
    25201 taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt
    25261 ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa
    25321 gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct
    25381 ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt
    25441 agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt
    25501 agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc
    25561 atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc
    25621 ttcaaaaata tgaagtagct gttgggagcc ataagggatc acttcaactg ggagtgggaa
    25681 cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta
    25741 cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc
    25801 agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact
    25861 ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc
    25921 tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc
    25981 ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat
    26041 gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc
    26101 tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata
    26161 aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta
    26221 acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat
    26281 atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat
    26341 gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa
    26401 ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa
    26461 ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta
    26521 gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca
    26581 cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac
    26641 ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta
    26701 cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc
    26761 cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac
    26821 ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga
    26881 tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa
    26941 aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta
    27001 acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag
    27061 tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg
    27121 ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc
    27181 ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag
    27241 caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca
    27301 ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct
    27361 gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag
    27421 taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata
    27481 tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact
    27541 atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga
    27601 tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga
    27661 agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg
    27721 aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg
    27781 aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat
    27841 ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg
    27901 accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca
    27961 caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag
    28021 agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa
    28081 aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg
    28141 aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat
    28201 tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg
    28261 ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta
    28321 aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat
    28381 ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg
    28441 ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc
    28501 gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata
    28561 tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga
    28621 gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac
    28681 ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag
    28741 atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg
    28801 gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta
    28861 acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag
    28921 gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta
    28981 caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc
    29041 ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct
    29101 tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg
    29161 tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta
    29221 agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag
    29281 attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg
    29341 aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg
    29401 gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg
    29461 accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa
    29521 aagctgaata atttcttaaa accggatagt cctgcaaaga gattatccaa agagactata
    29581 tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt
    29641 gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta
    29701 aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac
    29761 acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa
    29821 acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg
    29881 gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg
    29941 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
    30001 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
    30061 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
    30121 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
    30181 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
    30241 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
    30301 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
    30361 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
    30421 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
    30481 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
    30541 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
    30601 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
    30661 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
    30721 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
    30781 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
    30841 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
    30901 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct
    30961 ttgttccgag cttttt
//
LOCUS       DAPBCS010000029        28637 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPBCS010000029 DAPBCS010000000
VERSION     DAPBCS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.029"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            7..624
                     /locus_tag="RD521_001631"
     CDS             7..624
                     /locus_tag="RD521_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771368.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="RD521_001632"
     CDS             1644..2813
                     /locus_tag="RD521_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7771369.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="RD521_001633"
     CDS             3286..3840
                     /locus_tag="RD521_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7771370.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="RD521_001634"
     CDS             complement(4013..4486)
                     /locus_tag="RD521_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7771371.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="RD521_001635"
     CDS             4498..5034
                     /locus_tag="RD521_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7771372.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="RD521_001636"
     CDS             5074..5445
                     /locus_tag="RD521_001636"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDT7771373.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="RD521_001637"
     CDS             5729..7000
                     /locus_tag="RD521_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7771374.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="RD521_001638"
     CDS             7245..8270
                     /locus_tag="RD521_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771375.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="RD521_001639"
     CDS             8564..9673
                     /locus_tag="RD521_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDT7771376.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="RD521_001640"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="RD521_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="RD521_001641"
     CDS             10668..11492
                     /locus_tag="RD521_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771377.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="RD521_001642"
     CDS             11524..12834
                     /locus_tag="RD521_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDT7771378.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="RD521_001643"
     CDS             12982..14241
                     /locus_tag="RD521_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7771379.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="RD521_001644"
     CDS             14251..15123
                     /locus_tag="RD521_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7771380.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="RD521_001645"
     CDS             15135..15965
                     /locus_tag="RD521_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771381.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="RD521_001646"
     CDS             16005..18188
                     /locus_tag="RD521_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDT7771382.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="RD521_001647"
     CDS             18249..19469
                     /locus_tag="RD521_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDT7771383.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="RD521_001648"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="RD521_001648"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDT7771384.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="RD521_001649"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="RD521_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="RD521_001650"
     CDS             22431..22640
                     /locus_tag="RD521_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7771385.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="RD521_001651"
     CDS             22633..22782
                     /locus_tag="RD521_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDT7771386.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22936..23601
                     /locus_tag="RD521_001652"
     CDS             22936..23601
                     /locus_tag="RD521_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7771387.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="RD521_001653"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="RD521_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDT7771388.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="RD521_001654"
     CDS             25304..25537
                     /locus_tag="RD521_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDT7771389.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="RD521_001655"
     CDS             25534..25941
                     /locus_tag="RD521_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDT7771390.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(27078..27596)
                     /locus_tag="RD521_001656"
     CDS             complement(27078..27596)
                     /locus_tag="RD521_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDT7771391.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            27963..28211
                     /locus_tag="RD521_001657"
     CDS             27963..28211
                     /locus_tag="RD521_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771392.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         9843 a         4314 c         5246 g         9234 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatagt tggattaaga taacgactat ttattttatc atatttttac
    26101 tataccctat acttttactt ggtatattta tgaataatcg caaatttacc cctatactac
    26161 tagtttgtaa gttagattaa taaaaatact aaagttattt gttcttttat taaatacacc
    26221 tacaacttat aaattgatgg tatttagata acacgaaaga ataaaaataa cagctgatag
    26281 taaagtttgt tgacgattgc tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc
    26341 tgaaaactta tgaaatgaaa gaccttttac atcttgcaga aatcggtact gggattaaac
    26401 taagcaataa aaactaatgt tcccctcatt ctttgaactt ctactatttg atttatttat
    26461 tctgataaat gacgatttca ccttcaggat cttcaaatcc cgtatagaat ttatttaaga
    26521 tctcttcagt atcagtaata ggataggcga taggccacat accctttgat ggttagaata
    26581 aactgctcaa aatctgtatc gtatgttggc tttagcatat taacaaaggc tatctccaaa
    26641 ttataaacca taagcgttga atcgttctta acaatttcaa ttcttcctaa cccaaagtca
    26701 gaagatataa ttaccggact tgccttaccc ttcatgtggg ttgttgagat tccatattta
    26761 aagggaattg tcggtactat cggcggtctg aaaaacatcc caaaaataaa aaaatctttc
    26821 ccctgctagt ctcctaagtc cctttgtagc ggatatggca ccataatctc attttatcga
    26881 agtgagtcat ggtttatctg ttggccaacc aaaattgata caatctaacg atgcaccctc
    26941 tacggcattc taggggagtg cacttgcatt cacccaataa aaaccggcag ttagcaaaga
    27001 acttatgtag tcgtatgttc ttactgttaa catacaagag gtctcctttc cacaattgct
    27061 tagcagtaaa cgggaattta caaagctaat ctcttccatt ttcttatctt tgttctaaaa
    27121 gtcccaaatg gagcaactgt atttacatga ataaatttat atacttccca tacagccgtt
    27181 ttggttgcct cgtctgccca ttttcttttg tgtgcagtga atagatcttc atcactcatt
    27241 gaatcaatca tcatgttaat atcatcaata ttacgattga gaatttcctc taattctttc
    27301 aatggtagat gtgcataggt atttgtaaac cacttatata actctcctaa ctgattccat
    27361 ttaaattgat cagaaggcgt tttaacagat aatccatttc tttcatcatt ttcccatttt
    27421 agaattaaat tcgtccaacc aacttgatat gctaaatttt cagcaggtgt tctatccact
    27481 tcctcaactc ttttatcctt caattcttca ggaatagttt caaattctaa aatatattta
    27541 gtataagtcc tatttatttc tttttttaat tcttctttat ttttatatgt tctcattata
    27601 ttacctccat aaattcctaa ttgtctcctt taattcatct ataatttcat ccacgatatt
    27661 ttaatccgtt ctacaatgca tatctgatat gcattgactg tctaactcat atgtgaatga
    27721 tttataaaat atcccaacgt ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa
    27781 aatacgaata tatttctttt gtagtaaaat cggactggtt tgcatatcat tttcttcgta
    27841 aatatcaaaa tcatgttaac tactatcaat tactaataaa aactaccact ttaagcgtta
    27901 agaactgtta agtttagtta agtgaggata gttcacaaaa tccattgtta aacttcacga
    27961 aagtggtaaa actcaaaatg ctttatatag gattacagca tcgctctttc ttcgattgcg
    28021 cgttgggtaa aaaatattat gaagtaaaaa ttgacgatga cacgttcctt acggctcatc
    28081 agatgaaaca gctacaaaaa cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc
    28141 gattgccata ttcacgcctc gctcgacgaa ctattaaagg tcaagattta cctgaagatc
    28201 atccttttta ggaaattgaa gctttttatg ttgcacaatg catttataat actacaatga
    28261 tgttttcacc agatattctt ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga
    28321 aaattcaccg cgaatttact aaaattatga ataattatgt taatcttccg aaattttttt
    28381 ccaaaattaa gggatcaagc tggtgttata gattgtttaa ggttggcaaa aaaataactt
    28441 ctttttaaac aattatagaa aaccgatact ctgttaagtt ggttgacacg gtgtcgattt
    28501 ttatgacttt cttattctga aacttcacta acggacagta cctaaaaagt ctatagtatg
    28561 gacgtttacg agtactattt tatcaaaaat gctgctcatt tcaacttata tgttttagtg
    28621 aactaccccc acttagc
//
LOCUS       DAPBCS010000030        28602 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPBCS010000030 DAPBCS010000000
VERSION     DAPBCS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.030"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            236..1876
                     /locus_tag="RD521_001658"
     CDS             236..1876
                     /locus_tag="RD521_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDT7771393.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="RD521_001659"
     CDS             2030..3352
                     /locus_tag="RD521_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDT7771394.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="RD521_001660"
     CDS             complement(3414..3845)
                     /locus_tag="RD521_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771395.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="RD521_001661"
     CDS             complement(3909..4745)
                     /locus_tag="RD521_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDT7771396.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="RD521_001662"
     CDS             complement(4935..5237)
                     /locus_tag="RD521_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDT7771397.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="RD521_001663"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="RD521_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDT7771398.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="RD521_001664"
     CDS             complement(5691..5957)
                     /locus_tag="RD521_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDT7771399.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="RD521_001665"
     CDS             complement(6211..6936)
                     /locus_tag="RD521_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDT7771400.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="RD521_001666"
     tRNA            complement(7302..7372)
                     /locus_tag="RD521_001666"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="RD521_001667"
     CDS             7489..8241
                     /locus_tag="RD521_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDT7771401.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="RD521_001668"
     CDS             8257..8736
                     /locus_tag="RD521_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDT7771402.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="RD521_001669"
     CDS             complement(8987..10567)
                     /locus_tag="RD521_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDT7771403.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="RD521_001670"
     CDS             complement(10560..11303)
                     /locus_tag="RD521_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771404.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..12255)
                     /locus_tag="RD521_001671"
     CDS             complement(11479..12255)
                     /locus_tag="RD521_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771405.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12503..13339)
                     /locus_tag="RD521_001672"
     CDS             complement(12503..13339)
                     /locus_tag="RD521_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDT7771406.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13350..14279)
                     /locus_tag="RD521_001673"
     CDS             complement(13350..14279)
                     /locus_tag="RD521_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDT7771407.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14471..14824
                     /locus_tag="RD521_001674"
     CDS             14471..14824
                     /locus_tag="RD521_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDT7771408.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14898..15554)
                     /locus_tag="RD521_001675"
     CDS             complement(14898..15554)
                     /locus_tag="RD521_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDT7771409.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15780..17714
                     /locus_tag="RD521_001676"
     CDS             15780..17714
                     /locus_tag="RD521_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7771410.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17800..19023)
                     /locus_tag="RD521_001677"
     CDS             complement(17800..19023)
                     /locus_tag="RD521_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7771411.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19017..20915)
                     /locus_tag="RD521_001678"
     CDS             complement(19017..20915)
                     /locus_tag="RD521_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDT7771412.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20909..22027)
                     /locus_tag="RD521_001679"
     CDS             complement(20909..22027)
                     /locus_tag="RD521_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7771413.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22110..22943)
                     /locus_tag="RD521_001680"
     CDS             complement(22110..22943)
                     /locus_tag="RD521_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771414.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22936..23853)
                     /locus_tag="RD521_001681"
     CDS             complement(22936..23853)
                     /locus_tag="RD521_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7771415.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(23876..24055)
                     /locus_tag="RD521_001682"
     CDS             complement(23876..24055)
                     /locus_tag="RD521_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771416.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(24080..25204)
                     /locus_tag="RD521_001683"
     CDS             complement(24080..25204)
                     /locus_tag="RD521_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7771417.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25387..26295
                     /locus_tag="RD521_001684"
     CDS             25387..26295
                     /locus_tag="RD521_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7771418.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26436..28049
                     /locus_tag="RD521_001685"
     CDS             26436..28049
                     /locus_tag="RD521_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7771419.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT         8893 a         5621 c         4868 g         9220 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    11641 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    11701 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    11761 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    11821 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    11881 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    11941 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    12001 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    12061 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    12121 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    12181 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    12241 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    12301 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    12361 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    12421 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    12481 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    12541 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    12601 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    12661 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    12721 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    12781 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    12841 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    12901 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    12961 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    13021 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    13081 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    13141 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    13201 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    13261 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    13321 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    13381 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    13441 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    13501 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    13561 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    13621 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    13681 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    13741 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    13801 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    13861 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    13921 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    13981 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    14041 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    14101 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    14161 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    14221 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    14281 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    14341 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    14401 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    14461 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    14521 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    14581 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    14641 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    14701 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    14761 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    14821 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    14881 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    14941 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    15001 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    15061 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    15121 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    15181 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    15241 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    15301 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    15361 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    15421 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    15481 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    15541 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    15601 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    15661 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    15721 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    15781 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    15841 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    15901 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    15961 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    16021 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    16081 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    16141 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    16201 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    16261 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    16321 aacaccctga cattttgatt ctagacgaac caacgaacca tttagatatc gagacacttt
    16381 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    16441 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    16501 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    16561 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    16621 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    16681 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    16741 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    16801 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    16861 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    16921 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    16981 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    17041 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    17101 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    17161 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    17221 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    17281 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    17341 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    17401 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    17461 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    17521 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    17581 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    17641 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    17701 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    17761 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    17821 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    17881 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    17941 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    18001 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    18061 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    18121 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    18181 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    18241 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    18301 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    18361 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    18421 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    18481 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    18541 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    18601 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    18661 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    18721 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    18781 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    18841 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    18901 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    18961 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    19021 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    19081 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    19141 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    19201 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    19261 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    19321 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    19381 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    19441 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    19501 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    19561 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    19621 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    19681 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    19741 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    19801 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    19861 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    19921 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    19981 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    20041 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    20101 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    20161 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    20221 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    20281 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    20341 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    20401 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    20461 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    20521 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    20581 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    20641 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    20701 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    20761 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    20821 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    20881 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    20941 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    21001 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    21061 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    21121 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    21181 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    21241 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    21301 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    21361 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    21421 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    21481 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    21541 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    21601 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    21661 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    21721 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    21781 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    21841 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    21901 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    21961 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    22021 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    22081 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    22141 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    22201 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    22261 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    22321 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    22381 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    22441 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    22501 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    22561 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    22621 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    22681 ctggctgtag ctgaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    22741 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    22801 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    22861 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    22921 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    22981 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    23041 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    23101 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    23161 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    23221 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    23281 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    23341 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    23401 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    23461 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    23521 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    23581 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    23641 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    23701 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    23761 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    23821 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    23881 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    23941 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    24001 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    24061 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    24121 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    24181 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    24241 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    24301 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    24361 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    24421 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    24481 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    24541 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    24601 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    24661 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    24721 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    24781 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    24841 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    24901 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    24961 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    25021 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    25081 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    25141 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    25201 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    25261 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    25321 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    25381 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    25441 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    25501 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    25561 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    25621 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    25681 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    25741 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    25801 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    25861 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    25921 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    25981 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    26041 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    26101 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    26161 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    26221 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    26281 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    26341 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    26401 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    26461 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    26521 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    26581 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    26641 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    26701 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    26761 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    26821 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    26881 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    26941 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    27001 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    27061 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    27121 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    27181 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    27241 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    27301 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    27361 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    27421 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    27481 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    27541 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    27601 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    27661 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    27721 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    27781 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    27841 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    27901 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    27961 acattacttt cagaagagga ttgaataatt atacttctgc ccgaatcttg gcaggtttgg
    28021 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    28081 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    28141 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    28201 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    28261 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    28321 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    28381 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    28441 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    28501 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    28561 tgtattaaat aatttaattt ttttgttcct atttatttaa ca
//
LOCUS       DAPBCS010000031        28496 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPBCS010000031 DAPBCS010000000
VERSION     DAPBCS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.031"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..881)
                     /locus_tag="RD521_001686"
     CDS             complement(<1..881)
                     /locus_tag="RD521_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDT7771420.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTV"
     gene            complement(1409..1528)
                     /locus_tag="RD521_001687"
                     /pseudo
     CDS             complement(1409..1528)
                     /locus_tag="RD521_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            complement(1532..3226)
                     /locus_tag="RD521_001688"
     CDS             complement(1532..3226)
                     /locus_tag="RD521_001688"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDT7771421.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(3283..3411)
                     /locus_tag="RD521_001689"
     CDS             complement(3283..3411)
                     /locus_tag="RD521_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDT7771422.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3410..3658
                     /locus_tag="RD521_001690"
     CDS             3410..3658
                     /locus_tag="RD521_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771423.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            3850..4215
                     /locus_tag="RD521_001691"
     CDS             3850..4215
                     /locus_tag="RD521_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDT7771424.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            4212..4577
                     /locus_tag="RD521_001692"
     CDS             4212..4577
                     /locus_tag="RD521_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDT7771425.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(4669..6096)
                     /locus_tag="RD521_001693"
     CDS             complement(4669..6096)
                     /locus_tag="RD521_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771426.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            6566..8401
                     /gene="tdc"
                     /locus_tag="RD521_001694"
     CDS             6566..8401
                     /gene="tdc"
                     /locus_tag="RD521_001694"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDT7771427.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            8706..10184
                     /locus_tag="RD521_001695"
     CDS             8706..10184
                     /locus_tag="RD521_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7771428.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            10150..11481
                     /locus_tag="RD521_001696"
     CDS             10150..11481
                     /locus_tag="RD521_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7771429.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            11595..14147
                     /locus_tag="RD521_001697"
     CDS             11595..14147
                     /locus_tag="RD521_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDT7771430.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(14154..15593)
                     /locus_tag="RD521_001698"
     CDS             complement(14154..15593)
                     /locus_tag="RD521_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771431.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            15568..15750
                     /locus_tag="RD521_001699"
     CDS             15568..15750
                     /locus_tag="RD521_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771432.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            15939..16301
                     /locus_tag="RD521_001700"
     CDS             15939..16301
                     /locus_tag="RD521_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDT7771433.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(16488..17345)
                     /locus_tag="RD521_001701"
     CDS             complement(16488..17345)
                     /locus_tag="RD521_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7771434.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            17465..18376
                     /locus_tag="RD521_001702"
     CDS             17465..18376
                     /locus_tag="RD521_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDT7771435.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18383..20029
                     /gene="mdcA"
                     /locus_tag="RD521_001703"
     CDS             18383..20029
                     /gene="mdcA"
                     /locus_tag="RD521_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDT7771436.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            20131..20430
                     /locus_tag="RD521_001704"
     CDS             20131..20430
                     /locus_tag="RD521_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDT7771437.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            20420..22096
                     /gene="mdcD"
                     /locus_tag="RD521_001705"
     CDS             20420..22096
                     /gene="mdcD"
                     /locus_tag="RD521_001705"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDT7771438.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            22100..22693
                     /locus_tag="RD521_001706"
     CDS             22100..22693
                     /locus_tag="RD521_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDT7771439.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            22717..23793
                     /locus_tag="RD521_001707"
     CDS             22717..23793
                     /locus_tag="RD521_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDT7771440.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            23818..24687
                     /gene="mdcB"
                     /locus_tag="RD521_001708"
     CDS             23818..24687
                     /gene="mdcB"
                     /locus_tag="RD521_001708"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDT7771441.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            24798..25562
                     /locus_tag="RD521_001709"
     CDS             24798..25562
                     /locus_tag="RD521_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDT7771442.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            26051..28144
                     /locus_tag="RD521_001710"
     CDS             26051..28144
                     /locus_tag="RD521_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDT7771443.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            28169..28471
                     /locus_tag="RD521_001711"
     CDS             28169..28471
                     /locus_tag="RD521_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7771444.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         9093 a         4925 c         5906 g         8572 t
ORIGIN      
        1 tggactgttc caaatttttt gacagcttgc gatgccatct gtaatggaga gattgtgccg
       61 ctttgtaagc ttactaaatt tttcgctttt tctttgacag gctctaatcc gtccattcct
      121 acacaatcga taatgacatc tgcgccacct ttagccagtt catacaattc tgttccagct
      181 tgtgccgtgt cttctaagaa ataagtttca accccattgt aattagccgc tttatctaga
      241 cggtgctgta ctggatctac tgcgattacg cgctctgctc cagccataac agcgaatttt
      301 tgagcgaata gaccgactgg accagaacct agaatcacaa ctgtatcgcc tttttttact
      361 cctgcgtttt caacgctcca ataagctgtg ggcaaaatat ctgatagaaa taaaactttt
      421 tcatcaggaa gatcattatc tggtacaaca aatgaagtag agtctgcata aggaactctt
      481 agatattccg cttgaccacc ccaatgatta ccgttcaact tgccaaatcc gaaaagcccg
      541 ccatataact gatctggatt tgattcatca cattggcttt ccatgtgatt caggcagaaa
      601 tgacaatgac cacaactaat attaaaaggg ataaccacac gttgcccttt tttcaatgtt
      661 tgaacttgag atcccacttc ttcaacgatt cccattggtt cgtgtcctac aacataccct
      721 ttttccaaaa ctgcttcacc atgatggtaa agatgcagat cggagccgca aatggctgtt
      781 gctgtgatac ggatgattgc atctgttggt tctaatattt ttggatcatc tacgtttctg
      841 atttccattt tctgttttcc ttgccaagtg actgctttca ttggagatgc tcctttcaga
      901 ttcataaaat tgtctgcatt attagtacta cagataattt cattttaaag gagatcttac
      961 ctttttgata atgaaacgct ttttttctta aatgaaacat tctatagtct tatgtatttg
     1021 tgttcggaga ggtgtgtttc cgatttttta gtgtaatggt aatcattgaa atagttgtat
     1081 ccgttagatg atgttggatt ggcaagagag taaacgttga aaaaatagtt gttttcaaaa
     1141 agaaacagac ataaataaaa agaggttatg acagaaggga tcagtttctc ggtattaagt
     1201 caaatttatg caaaaacgtg aggaaccatt ttcacaaaat ttttcttcat actcgaaact
     1261 aaacgccttt ttcataacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
     1321 agtctaacca agtcaaaaac ataagaagct gttttagctt ggctgctctt attacttgat
     1381 gcagggcagc tttttcataa cctcttggta aacggtgttc aaaagcttgc atctgcggta
     1441 ataagtccaa ccaaatcaaa aacataagaa gctgttttag cctggttgtt cttattactt
     1501 gatgcagggc agctttttca caacctcttg attattcaaa catttttttt gcggcacgag
     1561 ctagagtagg aatatctttg tcgtaacgcg gtgttttgac aagacgggac ataggaatac
     1621 ccgcaaaatg gaaagcagcg atttctccag aacgtacagc actttgttca gtaaagatca
     1681 tctgataagg ctgctcgaca aattctcctg taaaggccag attggtcgaa tgtttaggaa
     1741 tgacttttgg tcgatcgctt ttggcacgat tattgaacaa cgcggaagca taaggcatat
     1801 agacagggat attatttatt acgctgtcga aaatttctgt ttctttttca cgaatattca
     1861 ctggtccagg atctactttt gaaagttgtc caatcagttc ttctagcatt tcttttccat
     1921 tcattttaat atactgttta tccacaaatt cgccacgtcg tcttggataa aggaaataac
     1981 cccaaataac tgtttcattt ggtttctgag acgtaaaatg cggttgatga tgcacgacaa
     2041 tagacatatt gacatctttt tggcctaacg gcgtgatcgg tgtggttgaa ataaatgaat
     2101 tcaaggcatt tcctggttct tgagtcgtga tacgggtgat ttcattcaac aacaaatggt
     2161 ttttcgtcgt cagggtaaag ctgacccatt cgcttgcatc tcgatcagca aagaatttgt
     2221 caggatttcc caagttgtag aaacgttcac tggctttttt ccagagacta gaagctgcac
     2281 cataatccat attttctaca gctggtgttt cgtaatctcc taaagtagct gaatctgtaa
     2341 tggacccatt agtgaaaata actgctgtat cttcatcgac cacaacgtat tcttcttctt
     2401 gagtcagtgt attggtaatt ttcaaaccag tcacagtgat ttcatcttgc atcggtgttt
     2461 ctttgaattc ccagtcagtg acacggcggt tcaaaatgat tttgcatcct tgttctttca
     2521 agtactcgat caaaggaagc ataatacttt cgtattgatt gtaacgtgtt cgattgacgc
     2581 ccactagatg ttcgatttga gtgaattcat aaatcatttg atgcatatag cgacgcaatt
     2641 cttgagcaga actacgtgtt ctgaaagcaa atgttgtttc ccacataaac cagaagtttg
     2701 tttcaaaaaa gtgtggatca tctttgaaat attcttcgat agtgatattg tctagttttt
     2761 cttcttctga atcaggcata gcaatcagtt tagtcagaag aatacggtct ttattattga
     2821 atcccagttt tccgccatca atgattcctt tgccaccttc catcaaacgt gctttgtcaa
     2881 aggttcggtg ttttgcatca aaatctctcg tatcttcttc agcagtcatc cctggctcag
     2941 taacagaagg tattcgactt aacagatcca tcaaatctac ataggtacgg taattaagca
     3001 tgcgaccgcc tcgagcaata tagccttttt ggttttctaa tggatggttt ttattccaat
     3061 attcatccac tacagtgtcg gtcggtgcac catcatttga accgtgatca tccaatgaat
     3121 aaaaggtgat ctgatcccct ttccatttgc cttcttgaat caaataaaca gccgctgcca
     3181 tattggcaat accggcacca atcattattg ctttactttt tctcattgtg attcctccaa
     3241 tgtcttccaa gtatgtccgt agtgattcaa ctaaatagga tgctagattg attcgaattc
     3301 atcatataaa tgactgtcat cttcgaatag tcctgtcttt tttcctagat atgcagcgcc
     3361 aagggcaagc aaacctaaac cgatgccgac tttgattaat tttttactca tgaaaaacgc
     3421 ctccttgttt tctctttcta tatccaagga tagttcgttt ttattagaat gccaaatgat
     3481 aggacgagaa gaaaaaggaa attttgacaa aaaagaagaa gtgtctgatt ttcctgtaaa
     3541 taaaaatagg gtaggtaaga agaaaagatt tgaggaggtg tatggttcta tttttagaaa
     3601 tttgggtaca aaaaaagcac cgagacagtg cttctttgaa aagctgatga cggactaagc
     3661 cttattttaa cttgctacgt tgttttggat acttccacga tttatggatg gagggagaaa
     3721 aatgggagag tgaaaaagaa aaatttaagc gaaccgttat cattagaaca acttttaaat
     3781 agacgaacaa ggtaactttc ttttacaata aaagaatagg aacaaattaa aagaaagtag
     3841 gtagcgtata tgtttcgctc aaatttaatt gaatttctag aatggatcat tgctatttta
     3901 aatatttgtt ctatcggtat cttaattgtc gggatcgttc ttgttctatt caaacttttt
     3961 gattggtctc atgtcaagaa aaactatgca gaacgtaata ctcgtaatgc agatacaaga
     4021 aaactgctag caagttatat tttgttaagc ttggaagtac tagtcgttgc tgatatcatc
     4081 gagtcagtta tcaagccgac ttggacagat attttgaagc ttgcacttat tattatcatt
     4141 cgaacagtca tttcttattt cttgaatcag gaaatagaag acaaagaaaa acaaagaaga
     4201 ggagaggtag gatgacgaca gagttgatgc agtttcttct tccatatttc gaaaaacttg
     4261 tactgggact tgatatcttt gctatctgtt tattggtttg gggtgtttgt ctatcaatca
     4321 aggatttttt gaaatattta tttgtccata caaaagtgca aagcgaccga gtcagcgaga
     4381 acaatgctat taaaaagatg ttgggaggct atatacttct aagtttggaa attcttgttt
     4441 cagctgggat catcgaatcg attatcaaac ctaccttgca ggatattttc caactagcgg
     4501 cgttggtagt tatccgaacc atcatttctt attttttaaa taaagagatc ggacccgtag
     4561 atgtccaaaa taagtgaggt tgtgagcctg acgtgtatca agagattgtt acagaaatgg
     4621 gcatcttcaa caaataagag gctgtgacat gatttttgtc acagcccctt atttgttttg
     4681 cacagctgaa atgatagctc gatcgatcgt attgatcgaa ataggaataa tattttcata
     4741 ttggaccgca tctggtgtat ataatgtacg ttcacataat acccggtttg aggactgctg
     4801 cgcatacttc tttatatcag atatacttga ataaattgtt ggtgcaactt gaaaatcgaa
     4861 agtagttaca gcttccaagg cttcccgata aatcagataa tgaacatccg aatttgtgac
     4921 gatatagata gtcagatgtt cttttgtcag acggagaatt ccgctcaatt cgatcacaaa
     4981 agcccgcatg caattaggat tcaaccgtag atcataaggt aactggctgg accagctgga
     5041 aaaaattgtc tggacttctt tatataaatt tgtctgttcc tcatttaaaa gataaaattt
     5101 ttctggcatg aataattgat agttatccca agccgaacgc atcagatgaa tcagcgcacg
     5161 ttcgaacggt tggtttccga ataattcttg attgaagtga gattcaaagg tagcaactag
     5221 ttcttttatt tcgggagtat tgtcaataaa cacttggtgt agctgttgaa agtcttccgc
     5281 aatcaactga taagaatgga aaccataatt acaaagattg aatagtgtca aaataaaggc
     5341 gaattcttct tttttataat aggtttggaa atcagtgttt tcccatgctg attcgacata
     5401 attgtaaata ggacgttttt tgatttcttc gataaatttt gaatctattg tcacagcaca
     5461 cacttgctgg cgtaggaaac tgatagcaaa accaatcgat aagatttctt tattttcttt
     5521 atcataaaat ctgccactat tttccgttac ggattcaatg aaacgatcgg cacgttcaaa
     5581 aaacgattca ggtagctccc aagaattaaa accgcctaaa cgatacgaca cattaagaag
     5641 cagcagacgt ctttccattt cagtgaatcg aggggagtag cggtcgattt cgatcccgca
     5701 gtcattaaaa taagctaata cttgtttttt taatgaataa gctttgctga cagagatgaa
     5761 ttcacattca gttaattgaa gataatcagg ttcatcttct tccaaataaa gcagacaagt
     5821 ttttaaaaac aacgaatttt gagacaattg ctgcatgatc ttgcatcgag gtatatgttc
     5881 tgaaaaatat aaagtgtaac ggtccttttc ccgatgtgta taagcgatct gttcttcaaa
     5941 ttcttttttt atgttattga tatcattgag taaagtggtt ttgcaaacat gcaggagatt
     6001 ggagagcttt tctagattga cttctttatt tacatagaat tgcccacata aaaacagctg
     6061 tcttaaaata tttttctcaa tatattcttc aatcatagta gagcctccgc cttgtatttt
     6121 tttgcacaat atgggaaata ttatgacatg ttttcttttt gaaatcatca gttaatttct
     6181 gaaaaagcat ataaatgccg ttaaaaaagg ttttttatgt tataaaattt ggaaaacaga
     6241 taattctatt aaaaaataaa ttgcgaattt ttaacaatat aaaaaaataa aaatatgttt
     6301 agtgaatgaa ggacagtcat tttatttgtt tgttcgcttt ttttaggtta tctattttag
     6361 aagactgatt tattgaaaaa ttgttcgctt ttttgcgttg aattatcaga aaaatccgac
     6421 tttatttatt tttgtttgta aaaatctacg tggatttgat gttataaaaa aaaaattctc
     6481 atttttggcg ctatagtgaa gaggtgttac agataaacac atgtatctgc aagacattga
     6541 ccaacaaaat atggagggaa gaaaaatgaa agatatggat atcaaggccg tctttatagg
     6601 cgacaaagct gaaaatggac cagtttataa aatgttgttg aacaagatgg tagatgaaca
     6661 tcttggatgg agagaaaatt atattccttc agatatgcct gcaattagcg aaggagataa
     6721 gctcactcca gattatctag ccacccgcga ccatatgata gaggtcttgg atgaagtcag
     6781 tcagcgatta cgcgcaggat cgatcccatg gcattcagca ggccgttact ggggacaaat
     6841 gaatgctgaa acattgatgc ctgctttatt agcttacaac tatgcgatgc tgtggaaccc
     6901 aaataatgtt gcattggaat cttctatggc aacttcgcaa atggaagcag aagtaggaca
     6961 ggattttgct tcattattca atatgacaga cggatgggga cacattgcag cagatggttc
     7021 tattgctaat cttgaaggat tatggtacgc ccgttgcatc aaatcgattc ctttagctgt
     7081 caaggaagtg ctgcctgaaa aagtgaaaaa aatgtctgag tgggaacttt tgaatttatc
     7141 tgttgaagaa attttggaaa tgaccgaaag ctttacggac gaagaaatgg atgaggtcaa
     7201 agcagcttct tcccgtagcg gaaaaaatat ccaaagatta gggaaatggc tggtccctca
     7261 aacaaaacac tattcttgga tgaaggcgtt agatatttgc ggagtcggct tagatcagat
     7321 ggttgctatc ccagttcaag aggattatcg aatggatatt aatgctttgg aaaaaacaat
     7381 tcgtgaatta gctggacaaa aaatcccaat tctaggtgtg gttgctgtcg taggaacgac
     7441 agaagaaggt caagtagaca gtgtagataa aatcgtccaa ttaagagaaa ggttaaaaga
     7501 tgaagggatc tatttctatt tacatgttga tgccgcatat ggcggctatg cgcgttcact
     7561 gtttctaaac gaagcaggag aatttgtgcc gtatgcttcc ttagctgaat tctttgaaga
     7621 acatcatgtt ttccaccact gcgtaacgat tgacaaagag gtatatgaag ggttcagagc
     7681 tatttcagaa gcggattctg tcacgataga tcctcataag atgggctatg tcccttatgc
     7741 tgctggagga atcgtaatca aacataaaaa tatgagaaat atcatttctt atttcgcgcc
     7801 atatgtgttt gaaaaatcag taaaggctcc agatatgtta ggagcttata ttttggaagg
     7861 atcaaaagct ggagcaaccg ccgccgcagt ctggacagca catcgtgtat tgccattgaa
     7921 tgtgaccgga tatggtcaat tgattggggc ttcaatcgag gcagctcaaa gattcagaga
     7981 atttctcgat catctgactt ttactgttaa aggaaaaaca atcgaagtct atccattgaa
     8041 tcatcctgat tttaatatgg tcaattgggt attcaaagaa caaggctgta cagatttgaa
     8101 cgctatcaat gaattaaatg aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt
     8161 ttatggtgaa cggtttatta cttctcatac tacatttaca caagaagatt acggtgattc
     8221 tccgattcgt tttgttgaaa gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa
     8281 aatcactctg ctacgtgcgg ctatcatgac tccttatttg aatgatgatc gaattttcaa
     8341 tttttataca aaaaaaatcg ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata
     8401 gaactgattt ttgtgtgaaa gaataacaag agacatatgg agaacagaca attatttgct
     8461 tcatcaaaac aattgtattt ccccctgttt ttcatatatt cctcgccaag atattctttt
     8521 ttataaaacg ttctataagt tggatcgttc gtttcatgta tcaaacccag ttcactaaga
     8581 tcgttcaatg gatgtttcgg tgatactggg ttttgtactg aatcgatgaa accttgtcat
     8641 ttctgttctt gaacaaaaat aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa
     8701 taaagatgaa agatactggt tcatcaaagc aaatcacgac catgaccttt atcggaatga
     8761 cctgcgccct tgtagcaagt gtacgaaata ttccagatgt agcagcaacg agctggacga
     8821 tgatttttta catgctggtt gcagtattat tgtacgcttt tccaatctca ttgatctctg
     8881 gtgaatttgc tggaatgttt ccccaaaaag gtggtccaga actttgggta tcgaatgctc
     8941 taggaaaaaa atgggggttc gttgtctcat ggctgttatg ggtccaaatg tttcctggga
     9001 tggtgatggt cgcttcagca ctcgctccgt tgtttggtaa tattatcgat aatgtgccgc
     9061 ttggtttgaa cagcaaattt acattagttg tcattttagt cgtttattgg atcatcactt
     9121 ttttgaattt gaaatttgat atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat
     9181 tgtacgtgcc tttgacgatg atgctcttat tagggtttgc tgcgtggatc aaagttggga
     9241 tcgtaccaac cggaacatta ggcagttttt catgggacaa attaatacca gatactacga
     9301 ctgcatcctc atttgtttat tttgcgccga tcatgtttat ttttactggt atcgaaatgt
     9361 cctctgtgta tatcactcga ttggaaaatc cagttaaaac gtatattcgc ggagtatttg
     9421 ccgcgttaat ttttatattt ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg
     9481 taccaaaagg acaaatggaa ttgaataata tcgcgcaatc tatttcgatt tattgccaaa
     9541 ttcttggttt gccacatctt attgtgaatc tgtttagttt acttgtcttc attggtgtag
     9601 cagtccaatt atctgcttgg gcctcaggac cagcaaagac agtgacagaa agtgctagaa
     9661 aaggtgcgta tccaccaaaa ttcaattttt ggaagacgaa tcaatttgac gtatccaaat
     9721 ccgttatttt gactcagtct gtcattatct caatctttgc acttttttat ttgctgattc
     9781 ctggcgtcaa tcaagcattt ttgatgctcg taaattcaac tactgttatc tactgtatcg
     9841 tctatgtcat catggggatc gcagttttga gattgcgtta tacacatgca aagttgaatc
     9901 gaccatttag aattggaaaa aaagagaaaa agagcaatct tggagtttgg actgtcgtta
     9961 ttgttttatt tgcggcaatc ggattttctg tcggactgac gatgaaagct ggaacatgga
    10021 tcaatctaat tgcagtcaca gccatcagtg tcattttgtt tgtagtacca ctctgtattg
    10081 aaaaaataaa gaaaccttct tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag
    10141 gtgaacttga tggaaaaacc ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta
    10201 atcgcattag gtggcgcaat tggaacagga ttatttttag gttcagcaaa ggcaattgca
    10261 tcagcagggc cagctgttat tctcgtttat ttgatatgcg gtttggctat ttatggtttg
    10321 atgcgggcca tgggtgattt atttttagca aatagtacat ttaactctat cagtgatttc
    10381 gttgactatt atttaggcaa aaagtgggca ttttttgtca attggactta ttggctttgc
    10441 tggcttggag ctggacttgc ggaattaaca gcaataggat tgtatatcca tttctggttt
    10501 gtacatgtac cagctttgat aagcggaggt agcgcactaa tgatcttaat ggcagtcaac
    10561 cttgttgcag tcaagtggtt tggtgaattg gaatcttttt tctcaatcat caagatcttg
    10621 gggattttcc tttttatctt attgggtata ggattgagtg tctcagattt tcatacccag
    10681 tctatcggaa tcaaagaaaa ttttggacag atcgatgaat tactaccgtt tggctttgct
    10741 ggaatggtag tctcatttcc tatggtttta ttcagttttg ctggaattga aatgatcggc
    10801 ttaaccgttg gcgagacaag tgatccgcaa aagaatttga aaaaagcaat caactgtctg
    10861 ccttggcgta ttctcttttt ctatatagga acgattttgt cgattttatt tgttgttcca
    10921 tggcattgtt tagatttaaa agaaagtcct ttagtgacta tgctgcaagt gatccattgc
    10981 caaggcggag cagcaattgt caatctaatg attttggttg cttctttgtc ctcagcaaat
    11041 agtgcagtct acagtactag caggatttta tatcaaacag caaaagaaaa aagtttgccc
    11101 cattggtttt gtgcgctttc taagaaaggc gtacctttac acggtatttt aattacagga
    11161 atgttatttt ttgccggtct ttgtctacat ttttttgtag ctgatagtcg attgtttttt
    11221 cagcttttgg caggcatgac aacgagttgt tttctatttg tgtggtcttc gatcttgtgt
    11281 gcacatgttc aatttattaa agaaaagaaa cggaacacta cacaaagata taaagatctt
    11341 gctttgttgg tgttttttac ttcaattgct ttcagcttgt tattcgaaag cacgacacgc
    11401 tttattcctt ttttattggt tatctggttt gtattcttaa attttgtata taaaagaata
    11461 agcaatagaa atatcagtta ggcccagata cattttgcaa aacgaattat ttcagacccc
    11521 cctgaaataa tcgatacgcc tgaaaaaact tccctgcgta taataacagt atcaattaaa
    11581 caggagggga taggatgaac tggtacagat tgacggtaga agatgtattg aaaaactatc
    11641 aaacatcaac agaaggcttg tcgcaagaag aacgacagaa acgcctgact gatcaaggat
    11701 ataatgaaat aaaagcaagg cagcagacaa aacgatggaa aaaaatagca aagcatttta
    11761 ctgatctgct gatgatcgtg ttgatcattg cctctttttt gaaatttgct tctagtgaat
    11821 atattgaagg aagtattatt ttattcgtag tgatcgtcaa tggactcgtt agttactggc
    11881 aagaaagaaa agcggaagaa tcattgaatg gtttgaagca gctgatggga caagaagcga
    11941 tagtactatc aaacgggatg caagaaaaaa tccctgcgaa agaactggta aaaggagatg
    12001 tcattgtctt acgtgccgga gatgtcgttc cagcagatat ccgattaatc gaagcacatg
    12061 atttattagt ggaagaatcg attttgacgg gagaatcaga agcatctgaa aaaagtcatg
    12121 ccgttttgac agaagaagaa tcgatcggcg accagaaaaa tatgggtttt tcaggaacgc
    12181 ttgtacaatc tggttctgca gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg
    12241 gtaaaatcaa tcaagcattg caatcggtcg aacaacaaac gacgcctctg attaaaaaaa
    12301 tcaaccgatt aaacaaacaa atttttcaag gcattctttg tttgagccta ttcttagtta
    12361 tttttacgac atttcgatat ggtatggatt ggcatatcct tctttcggct acgattgctt
    12421 tggtggtgtc aatggtaccc gaaggattgc cagctgtttt gaccatgatt ctatccgttg
    12481 gagtacatga aatggcaaaa gaaaaggcga tcatcaaagg acttccttcg gtagaaacat
    12541 taggttctat gacagtgatc tgttcagata agacaggaac attgacaaaa aatgaaatga
    12601 cagttgtgga tgtagctgca aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc
    12661 aggaattaaa aacaaaagaa gaacagaaaa cagaaaattt aagtggcaat ccgacagaag
    12721 ttgcgctgat tcgacacaca gaaacagcca atctaccttt gaaaaaaata gaagcaaaga
    12781 ttccgttcag ttctgaatac aaatatatgg caacgatgca tgcggaggaa aaaggagcgg
    12841 tgatttacgt caaaggtgca ccagaagtat tatttgctaa atcaactctt tcttctgcag
    12901 aacaagaagc atggagtcag acagctgcag aatttgcgag aaaaggtcag cgtgttttag
    12961 gatttgctta taaaaaagta gattcgaaac aggaactcac gcatgagact ttaacccaat
    13021 taacttttgc agggatcgcc ggattgatcg atccgccaaa agaaagtgca gtcaaagctg
    13081 taaaggaatg ccaacaagca gggatctcag tgaaaatgat tacaggggat cacaaagaca
    13141 cagcaaaagc gattgcggaa caaattggct taaaacatac tgccaaagtt ttagaaggga
    13201 tcgatttgga tcttatgtct gatgaagagt tgattcagca agtgccaata gtcgatgtct
    13261 ttgctcgaac gacaccagaa cataaactgc gaattgttaa agctttgcag aaaaatggag
    13321 aaatcgtcgg aatgaccgga gacggcgtaa atgatgcacc tgctttgaaa agatccgatg
    13381 taggaattgc tatgggaatc aaaggtagtg aagtaagcaa acaggcagct gatatggttt
    13441 taggagatga taatttccat acgattgcaa aagccgtaaa agagggccgg cggattttgg
    13501 ataatttgca aaaaaccatc aatttcttct tgccaactgc attagctcaa ggattgatct
    13561 tgatttgggc acttatgctg aatcgcccgc tacctttgtc cccagtacag attttatggg
    13621 tcaatatggt aacgacaatc actttatctt atgcactagg gttcgagtcg ccagatccag
    13681 aaatcatgaa gcgcccacca agagatccaa agcaaggaat cctatctggt tatcatttgt
    13741 ttcggatcat ctatgtctcc ctactgatca tggttcctgc ctatttattg gctatgcagt
    13801 ttgaagggca aagcttacag caaaccattc ttctgcaaaa tatcgtctta gctcaagctg
    13861 tttacatgat caattgcaga gagttgtcca agccgtcatt gaaccaaggt cttttgcaaa
    13921 acaaagcgct ttttctttca ttaggtatcc ttgctttatt gcaagggatg gttatttttc
    13981 taccagtcgg ccagcagctg atcggtacag taagtttaac gatgacacaa cagttattga
    14041 ttggagcaaa tgtgattctt ctattcttag ttgtagaaat agaaaaaggg atcatgaaca
    14101 aactgtttcg caaaaaagaa aaaacagcta gagaatacag tcgctagccg ttactataaa
    14161 aaagttttga aagtgagttc agtcaaacga ttgagctcat ttttcgttgg aatagaagaa
    14221 atgtatacaa tagggatatc tagagaatct agagggaaat tggaaatcaa gatatctgtt
    14281 ttttcaaaag aaatagtgga aagatcggtt agttcgatag gtaagaagca gacacgtttt
    14341 cctaagtaat cttttaattc ctgctgcaag aacgccttcc atgaaggttc tccttggaaa
    14401 ataaaataga cattgaacaa attcggtttg atagaaaaaa tcgtttgttg gatcagtaga
    14461 gacaagagtt ggagaaagta atttggttca ttgatttcta tttctttttt tagatcgttc
    14521 agtaaattct ctttctgttg atacaagtgt gcatattgct gatcatatgt ttctaaaaaa
    14581 cgatggtatt gacggtccaa tgcttcaatc aatatcggcg attcatgata ttttaatatg
    14641 aggagagcca agttatcagt cagagatgtt ttttttagtt gatcaagaga cagctctgaa
    14701 gctagttcct ctacaaacgt ttcgaggacc tctaaaaatg gagaatgatg caggcagcga
    14761 gcggtttctt gtccatagtt attgttgtaa ttccaacttt ccagaaagca gaaagaagca
    14821 aaagaaagct cagtgtcttt aagataaata ccttccgtta aatacaattt tttcaatgca
    14881 gagtcataca gggaggagag tgtgctgttg gtatggatag aagaatctaa tcggcaattc
    14941 gcttttatac ggatcgtatt gataaagaac caacatgttc cgaaaatctc ttctgtttca
    15001 accgctagtt cagtttgact gaggtttctt aaaaattgaa aataatggga ttcatggatc
    15061 gaataatcct cgaaaaaata gttgttatga gtgaaaggaa tcagcaatcg atgataaaaa
    15121 gtacgaatcg cacgctcttt tccaagaata caagctgtat atttcgtcaa atcgatctgc
    15181 aagtcgtaac ttgctaaacg ctgattgatt ttagtcacat gtcttttcaa tgtcccataa
    15241 gaaagcccat ggtcggctag aaaatcaggg acagcaactt tttttgtaaa aagcagattt
    15301 ttcagcagtt cgacactgac tgattgtttg aaaaaaatct cccaaaggtc atttgttaat
    15361 aaattttgct ggttttgcaa actgattcct gcttgatcgc tgatgatgtc ccagccttcg
    15421 ggcagctgag agcgaataga ttgaaggtca gaaaaaatcg ttcgttcggt tgtccctatg
    15481 atttcagcta attcatttac ggttatcttt gtatgattca gcaattgttc tactaaagaa
    15541 acatgacgtc gaatatcttt ttcggtaatg atttttttca ttagtgaata catcaatcag
    15601 actcccttct attctaagaa tacggagaaa ataaacggtg gtcaagtttc aactgatttt
    15661 tcaatcattg tttatttttc tggagcattt actgatgata agaatttcat catgaacttg
    15721 aaaaatgata ccactaaaat caaaaaatag gtgggcaaac aataataaac attttaatag
    15781 tagatggtag caagaccaac acaggcggat tgaacattta agagttgata aaaaagaaaa
    15841 atatcgaagt atttgattag caactacttg tattcatttt tctattctta cgtggtatct
    15901 ttgaataaag cgaatcaata agaaagagat ggaagcagat gctaacgaaa aaagaactaa
    15961 tcgatcacat caatgaaaca tacaatttgt ctccagaata tatttttaaa aaatttcctg
    16021 attattgcgt attcagacat caaaaaagca aaaaatggtt tggggtcctg atgtctgttc
    16081 cagaagaaaa aatctatgat gatgcaagtg accataggat catcaatatt cttgatttga
    16141 aggtcgatcc tgaacttggc ggaattttgc gaggaaaaga ggggtatttt ccagcctatc
    16201 acatgaataa agaacattgg atctctattg atctacgtaa aatagaaaag ctagatgaac
    16261 tagcagattt gattgatgga agttttaaat caacgcaatg aatgtctagg acaatagaga
    16321 agaggttgtg acagaaatgt ttagcttcaa gaaataagaa ggaatttacg aaaattgctt
    16381 ctcaaatttt tgggaaattt cggcttattt tctaagaaat tacttctgga acaccgtttg
    16441 ttcgaaaaaa gagctgcgac aagaattttg tcccagctct ttttttttta ttttcctaaa
    16501 taaaattccc gcatcgtttt tgcaatcatt ttgatcaaag gatgatcttc tgtgaagcta
    16561 gaagataaat aaattgttcg tgtcaagggc aaatctttga tttcccgcaa gacgaaagga
    16621 tcatttttat cgtaagacca agaaactttt gggaaaaagc taacagctaa tccttgcttg
    16681 acaatagaac gtaatgtcgc aggatcatct gtcgtcgaac tgatcgtcaa ttcgatactt
    16741 ttcgcttcaa gtgctgcatc cagtgtatga cggaaggcgt tgctctttga cagcatcaag
    16801 atgggggtgc gttggatatc ttctaattgc agatgttctt ttttagcaag aggatgtttc
    16861 ttaggaatgg cgacataaac aggttctttc atgataggaa tattggtcat gccaggcttc
    16921 atctccgaag tgatgtacag tacaggatcg gtaatatcat ctggtaaatg atgttgcatg
    16981 atcgttaatt ggatatcagg atgcttttcc ataaaaattc ggaccaagtt cggaatagaa
    17041 acagaggcta cctctaaata caatatgacg gaagtaacga tcttctcatt gaccgaagac
    17101 aataatttga ttccttcttc taatgtgtct aatgtcttga ctgcatatgg ataaaattct
    17161 tctgcataaa cggtcgtctc aatgtttctg ccgctatggt aaaacaaagg gacacctaat
    17221 tcgttttcca aatgtttgat cgcacggctc aaggcaggtt gagaaataaa taattctttg
    17281 gctgcttggg taacattttt tgtttcgaat acttttttaa aatactgcag atgaagcaat
    17341 ttcataatta tccatcccat ccataccttt ttgttatggt tttttctttt tttggcattt
    17401 tttattatag cagaaattga ctaagatagc agtggaaaaa gtaagtgctg aggaggcgag
    17461 gctcatgagt acaggtttct tatttcctgg acaaggcagt cagaagttag ggatgttagc
    17521 ggatatccct gaaatgtaca tggaacgttt agaaaatgtg acggggtatc ggttagttga
    17581 taaagaagaa aactatcaag atacggtttt tatccagcta gcattactga caaaagcagt
    17641 attttattta gatgaaatgg aaaaaaacgg tattcagcct gatattgcag caggacattc
    17701 gattggtgct ttttgtgcag ctgttgcttg tcgatctttg agatttgaga atgcagcgga
    17761 actagtctat caccgcgcag tcttaatgaa agaagcttat ccaagcggtt atgcaatggg
    17821 cgtagtagta ggtgtgacga gaagcgaagc tgaggaaatt gtagcaaaaa catttgatcc
    17881 tgaccatcca gtttatctgt caaatgaaaa ttgtccgatg caacatacgt tatctgggag
    17941 tatcgaaggt ttatccgtta cgttggcaga agccaaacgg caccaagcgc aaacagcgaa
    18001 gctgctgaaa gtccctgtgc catctcactg tctcttgatg aatgatacgg tcaagaaatt
    18061 caagccgtat atgcagtctg tccatctatc tgatgctaga tgtttgtata taaaaaatac
    18121 ggatggaaga gccactcagg acgcagaaga gattcgtaaa gatttgttgg aaaacctagc
    18181 tttccctgtg cagtggaacc aaatgatgga cttggcaaaa gaattaggaa tggatctgac
    18241 gattgaattt ccccccggca atactttgac aaagctgatt catacaaaat ttggtgtaga
    18301 taaagggatc cgtacgatca atctagacca acatggtatt gatgacgcca cgtttctata
    18361 tcaaaaatgg agatgactaa ttatggaaaa accaaaccgc aactgggctt ctaaacgcga
    18421 aaaaaaagcg cagcgattgg aaaaaatcag caacttgctc aatggcaagt atgtagaaac
    18481 tgaaaaaatc gtagaagtaa tggaaaaact gattgctccc ggcgacaaag ttgtattaga
    18541 aggcgacaat caaaaacaag caagttttct ttcagaaagt cttgaaaaag tagatgcaga
    18601 aaaagtgaat gatctgcatt tgatcatgtc tagtatctca cgaccagaac atttgaatat
    18661 ttttgaaaaa ggaatcgcaa gcaagatcga tttctcttat gctggtgcac aaagcttgcg
    18721 tgtcgcccaa atgattgaag acggcacaat gaaattaggt gatatccata cttatttgga
    18781 actttatgga cgcttattca tcgatttgat tccaaatgtt gttttagtag ctgcagataa
    18841 agcagataaa gacggcaact tgtacacagg tttcaatacg gaagaaacac caacaatcat
    18901 cgaagctgca gctttcaaag atggtatcgt gatcgttcaa gttaacgaag ttgtcgattc
    18961 tttaccacgt gtagatatcc ctggtgactg ggtagacgtt gtcgtgaaag cagataaacc
    19021 ttatcaatta gaagctttat tcacacgaga tcctcaaaac atcacagaat tgcaaattct
    19081 aatggcaatg atggcaatca aaggaatcta cgcagaacac ggcgtacaat ctttgaatca
    19141 cggtattgga ttcaatacag cagctatcga attgttactg ccaacttatg gtgaatcact
    19201 agggttaaaa gggaaaatcg cgaagaactg ggtattgaac ccacatccaa cgatgattcc
    19261 agccatcgaa tcaggttggg ttgaaagcat ccatagtttt ggtggcgaag ttggtatgga
    19321 aaaatatatt gctgctcgcc cagatgtctt ttttgtcggt aaagacggca cgatgcgcag
    19381 taaccgaaca ttatcacaag ctgcaggaca atatgccatc gatatgttta tcggttcaac
    19441 cttgcaatta gattacgaag gaaactcttc taccgtaaca aaaggacgtt tatctggttt
    19501 tggcggtgcg cctaacatgg gacataatcc tggcggtcgt cgtcattcta caccagcatg
    19561 gcaatcactt cgcgacaatg atgatccatt agggaaaggc caaaaattag ttgttcaaat
    19621 ggttgaaaca tatggttcaa ataaaaaacc agtctttgtc gaagaattag atgcactagc
    19681 tgttcaaaaa caagcaggct tagccaatgc accagtgatg atttatagcg aagatactac
    19741 acatatcgtg acagaagaag gaattgctta tttgtacaaa gcaaaaacaa aagaagaacg
    19801 acaagcagca attgcagcaa ttggcggtgt tacacctctt ggaatgacta gcacgaaaga
    19861 atcactagat agcttgcgtg cagccggcat cgtgaaatta ccagaagatc taggcatcaa
    19921 acgtagtgaa gcgaaacgtt cattattagc tgcacaaacc atcgatgatt tgatcgaatg
    19981 gtcagatggc ctgtatgaac caccaatgaa attcagaact tggtcataaa attggcgaga
    20041 aaaacctttt gtcgccattt acccagatgg aagaagaaag acgaataagc agaacgtgta
    20101 tgctctgttt tacaaaaagg ggagagatcc atggaacaat tagcttataa atatccaacg
    20161 aaacagccga tcaataagaa aatccatgta ggagtggtcg gttcaggaga tttggaaatc
    20221 ttgatgtttc caacagaaaa aacagaatct actgtaaaaa tttgtacagg aagcgatggc
    20281 ttcggcgaag tatggaacaa tgtattaacg agattttttg atcgttatcc aatttcagca
    20341 gatatcgtga tcaatgactt cggtgctaca ccaggagttg tgtacctacg attgacgcaa
    20401 gcaatggagg aactttcaga tgaaaaatag ttttgtagaa ttaaacgcca gagaacgagt
    20461 gaacgctcta cttgacaatg gacgtgaatt gattgatcca ttcgatcaaa tcatcgcgcc
    20521 aagtttacca gcacaaggaa tcgtccctga aagtgatgac gggatcgttg tcagccgtgg
    20581 cacgatcaat ggcaaacaag cagtagtaat cgctatggaa ggaaaattcc aaggtggcgg
    20641 tatcggtgaa gtttccggtg caaaaatcgt tgctgcactt gaacatgtgt tagaagaaaa
    20701 taaaaaagga aatgagatct ttccagtcat tgtactagat acaggtggtg ttcgtttgca
    20761 agaagcaaac tacggactgt tatctatttc agaaatcgga aatgtaatca ttgctctaaa
    20821 acaatatgta ccagtcatcg gtttgattcc aggacgtgta ggatcatttg gcggtatgtc
    20881 gattacttca gcattaatga gttatttaat cgcaacaaaa aaagcgagag tcggtttgaa
    20941 tggaccagaa gttattgaac aagaagcagg cgttcgagaa tttgattcta gtgacaaaga
    21001 cttgatttgg aatacattag gatctcgtca acgggtagaa tcaaaaatca ttgatgaatt
    21061 agtgactgat agtgtagaag caatcaaagc tgctgtcatc gcagcaatgg acgaacgaaa
    21121 agacacgcat cgcagtgaaa gctcagcatt ttatttatca ttacttgatt ctttagatct
    21181 ttcaaaacca atggccattg acgattacaa tgaagcaatt gctaaccatc aagcaaaatc
    21241 aattgactta ccagaagtag cagaaggaaa tgaaccagct gcagcaagtg ttggttacga
    21301 ttggtttgcg gcactaactg gcatgaaaaa tccaacttct tctattagta ctgtgtatta
    21361 tggtaaatca agtaaattca tagaagaagc catcgtgaca tcaatcgtac ctgatcctaa
    21421 aaacccgatg taccgtgtac gaaacggtga agttggttta gttgaaggtt tccgcatggg
    21481 acaaattttg gatcacgttt acgaagaaga taaagacaaa aaagtgaaac ggccaatcat
    21541 cgttgtgatc gatgtaccaa gccaagcata cggctataac gaagaattga tcggtatcca
    21601 tgtggctttg gctaatagtg ctgctgctta tgcaaaactc cgtcaagctg gacatccagt
    21661 tattggattg attgcaggaa atgctatttc aggtgcattt ttagcacatg gattacaatc
    21721 aagccgcttg attgctttaa acaataaaga aattaccgtc caagcgatgt ctaaagaaag
    21781 tgctgcacgt gtgacaaaac gttcggttgc tgaaatcgaa gcagctgctg aaaaagttcc
    21841 agctatcgct tacgatatca ataattatac aaaactagga gcgatctatc atttcctaga
    21901 agatatcacc gaccaagctg cttcagaaca aaatgtcagc aaagtcatcg aagcaatcaa
    21961 tgaagccatt caagatgtcc gcaatacaaa tgacacgatg ctgacaaatc gatacacaaa
    22021 cgaaacagcc aaaacattcg gacgtgtcga aacaaataaa gtccgtgcca aaatggacga
    22081 agaatgggat gcataaaaaa tgattcaagc acatgatatt gtcctttttg ataaacatga
    22141 aatagaaggg ttcaccttgc ctgaatgggt cgttctaact gaaaagatgt tcggaacagt
    22201 tcgtcgtgcg tcgacggatc acgcagaaat actaccgatt ggtttacgtg gacctgcccg
    22261 caatcagaga tggtcaacag aaatctcaaa acatgccgtc aaacaagtca ttcatccgtg
    22321 ggacatcatc gaacaagaat cgtatagaca agagaaaatc atggattatc ctgtttatca
    22381 gcaatttcat gctgctagaa acatattatc aggatgtaaa tggggaatcg gcggcagctt
    22441 aggatttgaa ttatcgactg ggattccggc agtaaaagaa accagtgatt ttgatttgct
    22501 gctgtacgca aattcaccga ttgaattgcc aattcaggta attcaatcgc atcctgcttt
    22561 ttttgagcaa ttcgataccc aagtagtgac tcggcaaggc ggtttttcac taaaagagta
    22621 tttgagagag ccagagaaaa aaattctatt gaaaacagtc gaaggaccta aattaaccaa
    22681 agaactttgg taataaagaa aaagagagag aaagagatgt ttgaaaacaa aaaaatcaaa
    22741 atcaacgtaa gtgacttcca gaaagtatca gctgtaacgg ctgttatgat gcaaacgata
    22801 ttgagctttg ctttggcaaa cagccacagc gcaacaaata cagcttttct tggtaccttt
    22861 tacgtcatcg ttaaatacac agcaccaatg tttatttttg caattgttta taatatggta
    22921 aaaacaagtc agcatttgtc ttattgggaa tttttgaaag aaaagttttt tgaactagtc
    22981 gttccgtatc ttctatggac gacggcttat ctttggctat ttccagcagt ccaacaacaa
    23041 actccgtata cggatactgc aagcttttta ctgaaatatg tcacaggtga tggagcgcct
    23101 catttatggt ataccgttat gatgctgcag atccaattgt tcatgccgtt ttttgtttgg
    23161 ctcggttaca aagtatttgc taataaaaaa tatgtatggc cagtattgat tggttcaacg
    23221 attttatatg tcgcatggta tctgttctat caaacacaag tattgactgg gccttatcat
    23281 gacagctggt acttattaga tcggtttgtt gctagtttca tgatttatgg ggtagccgca
    23341 tttgtttatc acgaaaaagt ctatcaatat ttggacagag tacgttatct tttcttacct
    23401 gttgccttag ttatcgcgtt cttttctgtc cgttcattgc tcgcacatcc aggagacttg
    23461 agctttgcga atgcacctta cttgaatacg atccaaagtt tatacagttt agtcatcatc
    23521 tttgctgtct tcataggcgc ttccaaaatg attgtaaacg attcgccaaa attgccattg
    23581 ttcaaatggt tgtctgttta tgcttatcgt acgtatttag ccaatgtatt tgttttccaa
    23641 gtactgttat tattgtttaa agatagttgg ctgcagttac cttcaggagt catgattctt
    23701 gtagcttatc taatgacggc aagttgtgca tttgctttaa gctggttact tcatatcatt
    23761 tgggtagcta tcaaaaaagg tttcagcaag taatagtagg aagaaggagt gagtgtcatg
    23821 ataaatgacg aacgaaaaaa agcagtgact cagcaactcg ctgcgttggt agaggaagca
    23881 ttgatcgcag aagtaacatt aagtccaaaa cctggattag tggatgcact ggatgcgggt
    23941 gcacattccg atatggacta tgctttattt cttaagtctg ctaagacgct aactcctttt
    24001 tttgaagaaa tggctcaaat agcatggcat catgctattg atcaggagtt aagagaaaga
    24061 attgctgaga ttgggcgaaa agcggaaaag gcaatgctga ttgctacaaa tggcatcaat
    24121 acccataaag gagcgatttg ggcaatgggg ctattgatca gtgtcattgc acaaaaaatc
    24181 agtatggaac agcctttttc tttatcgaaa atacttgatg atgtcagcaa gcttgctgct
    24241 tttcctgata caaaatatcg tcataaaaaa accactcatg gtcatagggt gaagcaaaaa
    24301 tatggcgtaa atggtgcata tgaagaagcg atattagggt atccgcatat caggcttgca
    24361 attcaagcag ctaaggagct agataatgat tcatatgtac agaaacaaat gcatatgttg
    24421 ttagttttga tgagttcgct agatgacaca tgcgtattgc atcgcagtga tcagaaaacg
    24481 ttagtggaaa tgcaaaaact tgctcatgct gctagtcaaa gtgctttacc aaatcgtcca
    24541 tttacagcac tgttgcactt ttgtcaggaa aaccaaatct ctcctggagg aagcgcagat
    24601 ctcttgtctg caagcttgtt catgctgaag atagaggaat tatggactgc cgacacatac
    24661 atttgtccga tgttactcaa tgactaaata agacaaaatt gctaaaaaaa cagtttatgc
    24721 atctggttat tgactcttcc ttttagcaag gctatcataa aaatagtttt ttgtaataaa
    24781 agataacgag gaagacaatg aaaaaaatca attatttagc aatcattttg ttagtgggcc
    24841 tatgtttgtt tgctctgccg acccaaggat tcgctgtcgc gggtggtcat ggtggaggta
    24901 catctagcgg tcgaagttcg ggcagaggtt catttggtgg tcgttcatat gggggcagtt
    24961 attttggcag tcgttcttac ggcagagatt cttatgggta ttatggtcca ggtacaggtc
    25021 cttctagtta tgagcaaacg ggatttcttg cattagctgt tttgtcagta ggaggggttt
    25081 ctgctttttc tatcgtcaag aataaaagac aaaagaaaag gctgaaagcg gagatattgt
    25141 cccgcatgtc aggaacggct aaagaaaaga aagcacagtt tctggaaatc caaaagatat
    25201 tcatgaagat ccaatccgct tgggaagaag aaactcctga aaaagccaga agctgttata
    25261 cagacaagct gtttgccgaa catcagaaag ttctgcaaaa aaacaaagca gacggtgtaa
    25321 gaaatcatac taaaaaaata actattcagg aagtaggaaa ttatcgacag atccacgaaa
    25381 atagttttgc catccgaatt gatttcagat gctgtgatta ttcagtcgat cgaaaagatg
    25441 ggcgattgat ctcaggaagt aagacacaac gacagtattt ttctcagata tggtattttg
    25501 atttttctga gaaagaaaaa agatggcaag ccgattttat tcagccgatt atgctggatt
    25561 gacaggaaat atttatgaac ggagttgtga caaaagcctt gtcgcagctc ctttttctga
    25621 ataaacggtg tgccagagat agcctatcgg caataaggca cgattttgta aaatatggga
    25681 agcgattttt caaaatcgct tcttatcgct caaggctgaa catctctgtc acaacctctc
    25741 cactaaatgg tgtttaaaag cttgcatctg cgataataag aacaaccaaa cgaaaaatag
    25801 gaagcactat tttagtttag ttgttcttat tacttgatgc aggacagctt tttcacaacc
    25861 tcttttttgt atagatgggc aaaatttttg taataatagg cgtatgtctt tttcgttgag
    25921 gagatgaaaa agcatatggc ttattttttg tttgctttgc ttactttctt tccacttcaa
    25981 gtggcagaaa tatctacttt ttattatgta agcggaacca tatacttgaa atcagaagga
    26041 gatcatttct atgaaaacaa gaacaattga aatacttcac agaatcatga cagcccaata
    26101 tccagtaaaa gcaaaacatc tagcaagtga gttcaatgtc agtcagcgga cgatccgtca
    26161 ggaagttctt gaagcgaata tctggttgag cagtaaaaag cttccggaga tccgtacaat
    26221 tcgcaacaaa ggttttttgt tgaagctgga aaaggaaaag caagctcgat tagaaactga
    26281 aataaaagat gtgaaagacg gctttttgaa tcggcgcgaa cgtatttttg atctagtttt
    26341 agccgttgct tacagccgaa tcccgatcca tctaaacaaa aaagaagaag aatttcaaat
    26401 ctctaaaagt acattagatg aagatatgag acggttacga gcaacactgg tcagttatgg
    26461 gatagagatc gtcagttatg gaaagcaagg actggtttat aaaggttcag aacgatctat
    26521 ccgtacgatg atctatgatt ttatcaatca gaatctggga atgaccgact tctttatgaa
    26581 tcaagatgca caattaacac cggctcaaaa aatctttctc cattattttc ctttggtgga
    26641 aatcaaaaaa atacaggaga tctattccaa taagttgttg aaacaagaag atgatattta
    26701 taaaaaacaa atcctgcttt ttacattgat ctggatccag cgaatcagga aacatgaatt
    26761 tatctctgcc attaattgga aaaatacaga aataaaagac agtagctttt ctgcatttat
    26821 tgaccacatg atcgaggaat ttcaattgga caatgtcccg caagtagaaa gaaattatct
    26881 tcgtttcact attgagacgt tcaatgctag agatatctcc aattcaatcg agtgggtaca
    26941 agctcaatta ctaacgatcc aattgatcca atttgtagag aacgagacac acgtaccttt
    27001 ccatctaaag gaagaagctt tattcgaaaa tctctataag cacatggctg cactgattgt
    27061 aagaatcaag aatcatatcc aagtcatgaa tccattgaaa gaaaacatcc gaacaaatta
    27121 tttcccaatc taccgcgcag tcactcattt tgttcctacg atcgaagaag taattggtgg
    27181 caagatactg gaggacgaga tagcttttct tgtcatccac ttttcgacga tcgccagtgt
    27241 aatcaagcga gacttgaatt atgtttataa atcagtcgtt gtctgtaacc atgggatggc
    27301 tactggaaat ctgttagcag agaatctgaa agaaaagttt cctcagatcg agattactgc
    27361 agttttaagt tcaaaagaaa tcgaattagt agataaattg gatgtcaatc tggttttcag
    27421 cacctttcaa ttgaattatc gaaataagcc gcttttagtt gtcgatccga ttttaactga
    27481 gacgaatatc ttggttatag aagacttttt gaaaagaaat gaagaactac agcggttaga
    27541 gcctaacagt catgaatcga cggagttatt cactcaaata atagatgtca tgaaagaaag
    27601 tggtggccga gtagatcgaa tcatctatgg caagttagaa aaattattcg aaacaaacaa
    27661 tttagaaatc aacaaaaggg agatccagcc tatgttaaaa gatattttga cggacagcaa
    27721 tatcatgatc aaagaaaaag ccactacttg ggaggaatcg attgaactag ctgctagacc
    27781 attggttaaa gaaaacattg tcgaagagcg ctatgtcgaa gcgatgattc gtgcagtgaa
    27841 tgattacgga ccgtatatcg tgattggcaa acacatggca ctagcccatg cccgaccaga
    27901 agatggggtc aataaattag gagtcagcgt agctacgatt gagcaaccaa tcgatttcgg
    27961 taatcctgaa atggatccag taaagattat tttttgtttg gcagcagtag actcctactc
    28021 gcatctgaac atcatgaaag aactaattga attgatcaac aacgaagaga agttggatcg
    28081 attgattgaa tgcacaaata tcgaagattt caaacaactg cttttttcag tagttgagaa
    28141 ataataaatg atacggggga gaacaaagat gaacaaattg aacattttat tcgtatgcgg
    28201 tgcaggttta ggaagtagtt ttgcagcaca gatgtctgct gaggatgttt tgaacaaatt
    28261 aggagtagaa gccaaactgg accattgtga tatttcttcc gcagtttcca tgaaaccaga
    28321 tgtgatcatc acagcagaaa attttcgttc gcagttcgaa aagttcgcag ttgatcctaa
    28381 aacggcgatg atctatttga aaaatattgt ttcaaaaaat gaaattgaag aaaaaatcac
    28441 cccagttctg caagataagg gagttatcta aatacaaaaa gaaaagaggg taaaaa
//
LOCUS       DAPBCS010000032        28459 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPBCS010000032 DAPBCS010000000
VERSION     DAPBCS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.032"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            8..81
                     /locus_tag="RD521_001712"
     tRNA            8..81
                     /locus_tag="RD521_001712"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="RD521_001713"
     tRNA            101..173
                     /locus_tag="RD521_001713"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="RD521_001714"
     tRNA            201..274
                     /locus_tag="RD521_001714"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="RD521_001715"
     tRNA            288..361
                     /locus_tag="RD521_001715"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="RD521_001716"
     tRNA            385..474
                     /locus_tag="RD521_001716"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="RD521_001717"
     tRNA            487..560
                     /locus_tag="RD521_001717"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="RD521_001718"
     tRNA            579..654
                     /locus_tag="RD521_001718"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="RD521_001719"
     tRNA            656..726
                     /locus_tag="RD521_001719"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="RD521_001720"
     tRNA            740..813
                     /locus_tag="RD521_001720"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="RD521_001721"
     tRNA            838..925
                     /locus_tag="RD521_001721"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="RD521_001722"
     tRNA            947..1018
                     /locus_tag="RD521_001722"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="RD521_001723"
     tRNA            1041..1130
                     /locus_tag="RD521_001723"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="RD521_001724"
     tRNA            1143..1216
                     /locus_tag="RD521_001724"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="RD521_001725"
     tRNA            1235..1307
                     /locus_tag="RD521_001725"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="RD521_001726"
     tRNA            1311..1383
                     /locus_tag="RD521_001726"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="RD521_001727"
     tRNA            1388..1458
                     /locus_tag="RD521_001727"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="RD521_001728"
     tRNA            1471..1544
                     /locus_tag="RD521_001728"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="RD521_001729"
     tRNA            1556..1627
                     /locus_tag="RD521_001729"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="RD521_001730"
     CDS             1893..3086
                     /locus_tag="RD521_001730"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDT7771445.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="RD521_001731"
     CDS             3216..4688
                     /locus_tag="RD521_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7771446.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="RD521_001732"
     CDS             4771..5559
                     /locus_tag="RD521_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDT7771447.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="RD521_001733"
     CDS             complement(5626..6294)
                     /locus_tag="RD521_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDT7771448.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="RD521_001734"
     CDS             6409..8121
                     /locus_tag="RD521_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771449.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="RD521_001735"
     CDS             8124..9893
                     /locus_tag="RD521_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771450.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="RD521_001736"
     CDS             complement(10098..10664)
                     /locus_tag="RD521_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7771451.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="RD521_001737"
     CDS             complement(10664..11620)
                     /locus_tag="RD521_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDT7771452.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="RD521_001738"
     CDS             complement(11620..12276)
                     /locus_tag="RD521_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDT7771453.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="RD521_001739"
     CDS             12646..14664
                     /locus_tag="RD521_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7771454.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /locus_tag="RD521_001740"
     CDS             15091..17505
                     /locus_tag="RD521_001740"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDT7771455.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="RD521_001741"
     CDS             17853..19406
                     /locus_tag="RD521_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDT7771456.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="RD521_001742"
     CDS             complement(19787..21571)
                     /locus_tag="RD521_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771457.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="RD521_001743"
     CDS             complement(21586..23307)
                     /locus_tag="RD521_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771458.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="RD521_001744"
     CDS             23407..24168
                     /locus_tag="RD521_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7771459.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            25135..25403
                     /locus_tag="RD521_001745"
                     /pseudo
     CDS             25135..25403
                     /locus_tag="RD521_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            26075..>28459
                     /locus_tag="RD521_001746"
     CDS             26075..>28459
                     /locus_tag="RD521_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771460.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVEL"
BASE COUNT         8981 a         5083 c         6031 g         8364 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag caaagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttgtaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcgaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt tttgcttata
    24661 aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag aaaaatcaca
    24721 aaggttattg ttatttttat atattctttt taagaatgat tattttttta tagaaatttt
    24781 aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata ttatagaagt
    24841 tgactggtag tgatttattt ttcgttgttt tataaaaatg ttattttttg gaaaaattca
    24901 caaatatgta acgaatgttt ttctggaatt tgctgtgtta aaaagcagta tgactaatat
    24961 aaatactatt gtcatttatg caatttttta tggatagtaa ttttagaggg gaatcaatga
    25021 aggaagaata gaagggaaaa atagttgcaa atacgccagt tagcaaaaat agcaatacat
    25081 cttcaagctt taaaattcga gagaatcatc ttggtagtgg aatcaactac aacaaaagga
    25141 ttaaaccaat taatagatag aattaccaat ggaaaaatag aaaaggtagt tattctatac
    25201 aaaagttgtt taattcgctt cggttatgaa cttagtgaaa ccctttgcaa tatatggtac
    25261 agaatataga aattattgac attacagaaa atgatgaaga tttaatacag attgtcacta
    25321 tttgtagata ccttttacaa ggaaaccgag ccaataaagc taagaagatg attaaggagt
    25381 tgatggaaga cgatacaagt taagaaagta aaactacgcc caaataaaga gcaaaaagag
    25441 taattggacg tggaaaatgc aagaaatgaa ccatcgattc ggtggacagt ttattagtaa
    25501 tgacggtttg agaacatcca tgacgagaat gaaggaacat cctaaatgcg tttagttgaa
    25561 ccaagtgcca aataatatgg ccaaataagc gatcagttat cgatgaatcc caagtattct
    25621 aaactatata ttcattttga tgctaagcat tgatatattt ttttgggatt gatgaaggtt
    25681 aaccaataca gggactcact gatgaaattc tagagtctga ccttggtatc aacgatttag
    25741 tgattgtttc taatggaaaa gtataaaagt atataagtac attaacaaaa taactattag
    25801 ggttacgctc cagcttgtgc aaggcactgg caggaggaat cgtgaaatca taattatggt
    25861 taagccacac ggacaactaa aagtcataca aagctatcga atcaatcaac agaagacaat
    25921 cggcaatcat tagctttgtt tacagcttac attgcaacgc ttgagttgaa ccgaaaatac
    25981 gagggaggta acgcagccat tttgagttgg ggaatctttt tagaaaatta tcgggtacct
    26041 tgttgtttag tcaaaattat aagggggagt aatagtgagg aacaaatttc ataaactgtt
    26101 aagtatgttt ctagttatgc ttattgtgat caatcaattt gggaacataa cggttctagc
    26161 tgagtcttta gcagaacagc cagagcgttc gctgacgatc gaagttgatc aagaggaggc
    26221 tgctttgggt gagcagttcc agttgcgagt cagaggaaca ccagacctgc taaatgcatt
    26281 ggtcattgat aagagtgagg atttgattga aggggaagtg aagcaactca atgattacga
    26341 acgtctgatt acttattctt cccagcattc aggtaaatac gaattgaccg caagaagcgg
    26401 tgatattcaa tcaaatacgg tctcaataga aatcagtgta ccagaagaaa gcacaagtga
    26461 gtcttttaat gtaccagaag aaagcacaag tgaatctttt aatataccag aagaaagctt
    26521 aagtgaatct tttaatgaag ccagatcatc tgagccatct ataacgcagg aatctcagat
    26581 tgaaagacaa gaggagctct tgaattatga catcggcatc tctgctgagc gccccacttt
    26641 gccgagcagg catacacaaa cgttaaaggg atacattcaa ggaggcgctg gcgatattct
    26701 tgaagaatat ggggagatgg tcgtacgagt tcctagagca tcgattggct taattgagtt
    26761 cattagagtg gaggtcgctg atgattcacc tttaaagcca ctggcatttt accaagaagg
    26821 cacagacgtt gtttttccat ttacctttga ttatgaagga ttaaatgaag ccgctcagtc
    26881 gtatacattc agtatcacgt ttgaagttgc tttctttcaa tcaggcggta ctgagccaaa
    26941 taatgacttt caagtgagtc ttacttatgc tgagcgagca gcaaacacgg aattttcaat
    27001 tacgcctatt ccttcatcaa gacccatttt ttcgaaatta tactatggct ctagagaaat
    27061 cgacagccaa ggaaatcttg ggaatctgct gattgacggg caagacgtga ccaacagtaa
    27121 taaatttatt ttgcaggtaa attatgaagg atcacgaaaa gacggtgtag tggttaccga
    27181 tgtactaccg caaggaacaa ctttggcacc gaatccgact acatggagtt ccactggaaa
    27241 tagagaacct atttcaggtg gggttcggat cgcagaagta acgaatccag gtgaagcaaa
    27301 cgttagaact cattatgttt ccgaggaatt tgtggacaaa gtatctttta ataaggaaac
    27361 gaatacactt gttgtggact ttggtaatct agcagagggt caatcgtatt ttattgaata
    27421 tgctcttgaa gtgaccgatg tcgacttgtt caatgaagga agacaagagg atgggttttc
    27481 tcggaatcga gcgagtatga cctacaataa tcaggtaatg agccgagact accgcttgag
    27541 aatccctgaa agtaatcttc cggcaatctc tttaagaaaa agtgtggaca aaaatgttat
    27601 caatctcaat gaaaatgaac taacgtatac tctgcatgtc tcgatgtttt ctggaacaag
    27661 caatacgctt gttattgaag atgttttgga agaaggcatg gaatttgtgt catttctatc
    27721 agatattccc tctttcattg atgccgaagt caataatgga atagttcgtt tcagtgcgaa
    27781 taatacgatt ggtggtccga caagttttac cctctctttt aaggtcaatg tagaggcgta
    27841 tgcggtcggc gatcaaattt taaactttgc aacgcttgtg aatagtgaca atcgattgaa
    27901 tacgagtact gtaagaacaa ggaaaatcga tgggcgtatt aaagtaatta aaactgatga
    27961 gaatggccaa aggctggcag gagcaaaatt tgaaatttta tcaagcgatt cacaagtgat
    28021 acaagaaggt ataacggcag aaaacggaga atttttaagt gaaccgcttc ctgtaggtag
    28081 ctatcaagtc cgagaaacag aagcaccgga agggtatctt ttagatgcga cggtacatga
    28141 tgtttttata aatgaatact ccatagaaga agtgacgtta accattgaaa acatccttga
    28201 tacgggtggt gttgaactaa ttaaacatgc tgaaggcagt ggtgaagtcc ttcaaggagc
    28261 tgtttttgag ttacagaacc gagaaggaga aacgctacaa accggtttaa ctacaggaga
    28321 agatggaaaa ctggcaattg acggtttagc accaggagca tatcagctgg tagaaactca
    28381 agcgccaata gggtatgaat tagatgcgac accaatcgag tttgagatcg aaagaagtca
    28441 gacagcggtg gtagaattg
//
LOCUS       DAPBCS010000033        28302 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPBCS010000033 DAPBCS010000000
VERSION     DAPBCS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.033"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(256..735)
                     /locus_tag="RD521_001747"
     CDS             complement(256..735)
                     /locus_tag="RD521_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7771461.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(808..1092)
                     /locus_tag="RD521_001748"
     CDS             complement(808..1092)
                     /locus_tag="RD521_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771462.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(1170..1364)
                     /gene="ssrS"
                     /locus_tag="RD521_001749"
     ncRNA           complement(1170..1364)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RD521_001749"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1436..2722)
                     /locus_tag="RD521_001750"
     CDS             complement(1436..2722)
                     /locus_tag="RD521_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDT7771463.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            2860..3531
                     /locus_tag="RD521_001751"
     CDS             2860..3531
                     /locus_tag="RD521_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDT7771464.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            3497..3985
                     /locus_tag="RD521_001752"
     CDS             3497..3985
                     /locus_tag="RD521_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDT7771465.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            3998..4945
                     /gene="prmA"
                     /locus_tag="RD521_001753"
     CDS             3998..4945
                     /gene="prmA"
                     /locus_tag="RD521_001753"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDT7771466.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            4946..5710
                     /locus_tag="RD521_001754"
     CDS             4946..5710
                     /locus_tag="RD521_001754"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDT7771467.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            5943..8156
                     /locus_tag="RD521_001755"
     CDS             5943..8156
                     /locus_tag="RD521_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDT7771468.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            8167..8613
                     /locus_tag="RD521_001756"
     CDS             8167..8613
                     /locus_tag="RD521_001756"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDT7771469.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            8784..10043
                     /locus_tag="RD521_001757"
     CDS             8784..10043
                     /locus_tag="RD521_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDT7771470.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(10132..11079)
                     /locus_tag="RD521_001758"
     CDS             complement(10132..11079)
                     /locus_tag="RD521_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7771471.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            11473..12765
                     /locus_tag="RD521_001759"
     CDS             11473..12765
                     /locus_tag="RD521_001759"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDT7771472.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            12780..14549
                     /gene="aspS"
                     /locus_tag="RD521_001760"
     CDS             12780..14549
                     /gene="aspS"
                     /locus_tag="RD521_001760"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDT7771473.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            14658..15533
                     /locus_tag="RD521_001761"
     CDS             14658..15533
                     /locus_tag="RD521_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7771474.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            15799..17682
                     /locus_tag="RD521_001762"
     CDS             15799..17682
                     /locus_tag="RD521_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDT7771475.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQISNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(17757..18041)
                     /locus_tag="RD521_001763"
     CDS             complement(17757..18041)
                     /locus_tag="RD521_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDT7771476.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(18031..18765)
                     /locus_tag="RD521_001764"
     CDS             complement(18031..18765)
                     /locus_tag="RD521_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDT7771477.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            18894..19517
                     /locus_tag="RD521_001765"
     CDS             18894..19517
                     /locus_tag="RD521_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDT7771478.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            19709..20830
                     /locus_tag="RD521_001766"
     CDS             19709..20830
                     /locus_tag="RD521_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDT7771479.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            20837..23965
                     /locus_tag="RD521_001767"
     CDS             20837..23965
                     /locus_tag="RD521_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDT7771480.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            23984..27178
                     /locus_tag="RD521_001768"
     CDS             23984..27178
                     /locus_tag="RD521_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDT7771481.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(27324..28031)
                     /locus_tag="RD521_001769"
     CDS             complement(27324..28031)
                     /locus_tag="RD521_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7771482.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT         9411 a         5054 c         6014 g         7823 t
ORIGIN      
        1 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
       61 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
      121 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
      181 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
      241 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
      301 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
      361 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
      421 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
      481 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
      541 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
      601 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
      661 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
      721 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
      781 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
      841 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
      901 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
      961 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
     1021 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
     1081 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
     1141 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
     1201 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
     1261 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
     1321 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
     1381 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
     1441 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
     1501 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
     1561 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
     1621 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
     1681 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
     1741 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
     1801 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
     1861 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
     1921 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
     1981 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
     2041 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
     2101 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
     2161 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
     2221 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
     2281 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
     2341 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
     2401 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
     2461 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
     2521 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
     2581 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
     2641 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
     2701 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
     2761 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
     2821 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
     2881 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
     2941 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
     3001 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
     3061 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
     3121 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
     3181 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
     3241 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
     3301 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
     3361 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
     3421 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
     3481 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
     3541 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
     3601 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
     3661 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
     3721 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
     3781 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
     3841 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
     3901 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
     3961 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
     4021 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
     4081 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
     4141 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
     4201 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
     4261 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
     4321 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
     4381 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
     4441 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
     4501 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
     4561 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
     4621 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
     4681 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
     4741 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
     4801 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
     4861 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
     4921 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
     4981 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
     5041 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
     5101 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
     5161 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
     5221 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
     5281 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
     5341 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
     5401 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
     5461 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
     5521 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
     5581 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
     5641 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
     5701 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
     5761 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
     5821 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
     5881 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
     5941 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
     6001 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
     6061 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
     6121 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
     6181 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
     6241 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
     6301 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
     6361 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
     6421 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
     6481 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
     6541 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
     6601 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
     6661 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
     6721 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
     6781 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
     6841 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
     6901 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
     6961 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
     7021 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
     7081 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
     7141 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
     7201 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
     7261 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
     7321 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
     7381 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
     7441 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
     7501 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
     7561 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
     7621 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
     7681 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
     7741 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
     7801 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
     7861 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
     7921 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
     7981 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
     8041 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
     8101 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
     8161 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
     8221 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
     8281 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
     8341 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
     8401 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
     8461 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
     8521 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
     8581 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
     8641 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
     8701 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
     8761 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
     8821 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
     8881 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
     8941 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
     9001 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
     9061 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
     9121 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
     9181 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
     9241 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
     9301 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
     9361 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
     9421 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
     9481 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
     9541 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
     9601 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
     9661 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
     9721 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
     9781 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
     9841 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
     9901 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
     9961 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    10021 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    10081 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    10141 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    10201 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    10261 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    10321 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    10381 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    10441 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    10501 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    10561 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    10621 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    10681 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    10741 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    10801 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    10861 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    10921 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    10981 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    11041 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    11101 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    11161 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    11221 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    11281 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    11341 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    11401 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    11461 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    11521 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    11581 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    11641 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    11701 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    11761 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    11821 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    11881 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    11941 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    12001 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    12061 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    12121 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    12181 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    12241 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    12301 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    12361 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    12421 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    12481 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    12541 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    12601 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    12661 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    12721 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    12781 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    12841 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    12901 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    12961 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    13021 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    13081 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    13141 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    13201 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    13261 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    13321 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    13381 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    13441 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    13501 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    13561 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    13621 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    13681 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    13741 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    13801 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    13861 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    13921 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    13981 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    14041 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    14101 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    14161 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    14221 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    14281 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    14341 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    14401 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    14461 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    14521 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    14581 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    14641 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    14701 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    14761 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    14821 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    14881 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    14941 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    15001 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    15061 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    15121 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    15181 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    15241 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    15301 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    15361 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    15421 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    15481 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    15541 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    15601 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    15661 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    15721 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    15781 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    15841 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    15901 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    15961 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    16021 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    16081 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    16141 agcttactgt atgtcaacaa ctggtggcaa atcagtaacg gtttatctta tttcgatcga
    16201 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    16261 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    16321 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    16381 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    16441 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    16501 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    16561 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    16621 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    16681 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    16741 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    16801 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    16861 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    16921 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    16981 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    17041 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    17101 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    17161 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    17221 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    17281 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    17341 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    17401 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    17461 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    17521 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    17581 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    17641 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    17701 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    17761 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    17821 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    17881 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    17941 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    18001 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    18061 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    18121 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    18181 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    18241 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    18301 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    18361 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    18421 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    18481 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    18541 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    18601 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    18661 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    18721 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    18781 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    18841 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    18901 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    18961 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    19021 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    19081 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    19141 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    19201 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    19261 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    19321 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    19381 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    19441 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    19501 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    19561 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    19621 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    19681 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    19741 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    19801 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    19861 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    19921 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    19981 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    20041 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    20101 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    20161 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    20221 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    20281 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    20341 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    20401 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    20461 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    20521 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    20581 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    20641 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    20701 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    20761 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    20821 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    20881 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    20941 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    21001 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    21061 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    21121 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    21181 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    21241 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    21301 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    21361 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    21421 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    21481 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    21541 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    21601 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    21661 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    21721 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    21781 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    21841 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    21901 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    21961 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    22021 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    22081 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    22141 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    22201 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    22261 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    22321 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    22381 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    22441 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    22501 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    22561 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    22621 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    22681 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    22741 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    22801 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    22861 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    22921 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    22981 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    23041 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    23101 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    23161 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    23221 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    23281 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    23341 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    23401 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    23461 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    23521 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    23581 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    23641 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    23701 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    23761 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    23821 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    23881 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    23941 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    24001 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    24061 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    24121 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    24181 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    24241 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    24301 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    24361 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    24421 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    24481 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    24541 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    24601 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    24661 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    24721 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    24781 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    24841 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    24901 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    24961 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    25021 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    25081 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    25141 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    25201 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    25261 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    25321 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    25381 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    25441 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    25501 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    25561 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    25621 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    25681 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    25741 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    25801 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    25861 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    25921 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    25981 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    26041 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    26101 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    26161 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    26221 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    26281 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    26341 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    26401 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    26461 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    26521 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    26581 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    26641 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    26701 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    26761 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    26821 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    26881 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    26941 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    27001 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    27061 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    27121 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    27181 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    27241 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    27301 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    27361 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    27421 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    27481 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    27541 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    27601 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    27661 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    27721 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    27781 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    27841 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    27901 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    27961 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    28021 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    28081 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    28141 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    28201 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    28261 taatgcaatc tctgtggaat tttcgaaaga tttatttttt ta
//
LOCUS       DAPBCS010000034        27888 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPBCS010000034 DAPBCS010000000
VERSION     DAPBCS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.034"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(57..173)
                     /locus_tag="RD521_001770"
     CDS             complement(57..173)
                     /locus_tag="RD521_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDT7771483.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..1347)
                     /locus_tag="RD521_001771"
     CDS             complement(418..1347)
                     /locus_tag="RD521_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7771484.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(1364..2386)
                     /locus_tag="RD521_001772"
     CDS             complement(1364..2386)
                     /locus_tag="RD521_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7771485.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(2383..4410)
                     /locus_tag="RD521_001773"
     CDS             complement(2383..4410)
                     /locus_tag="RD521_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771486.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(4407..6860)
                     /locus_tag="RD521_001774"
     CDS             complement(4407..6860)
                     /locus_tag="RD521_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7771487.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(6844..7239)
                     /locus_tag="RD521_001775"
     CDS             complement(6844..7239)
                     /locus_tag="RD521_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7771488.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(7311..7949)
                     /locus_tag="RD521_001776"
     CDS             complement(7311..7949)
                     /locus_tag="RD521_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771489.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(8005..8499)
                     /locus_tag="RD521_001777"
     CDS             complement(8005..8499)
                     /locus_tag="RD521_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7771490.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(8566..9345)
                     /locus_tag="RD521_001778"
     CDS             complement(8566..9345)
                     /locus_tag="RD521_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDT7771491.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(9387..9608)
                     /locus_tag="RD521_001779"
     CDS             complement(9387..9608)
                     /locus_tag="RD521_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771492.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(9605..9895)
                     /locus_tag="RD521_001780"
     CDS             complement(9605..9895)
                     /locus_tag="RD521_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771493.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(9892..11076)
                     /locus_tag="RD521_001781"
     CDS             complement(9892..11076)
                     /locus_tag="RD521_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7771494.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(11258..12661)
                     /locus_tag="RD521_001782"
     CDS             complement(11258..12661)
                     /locus_tag="RD521_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDT7771495.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(12683..13456)
                     /locus_tag="RD521_001783"
     CDS             complement(12683..13456)
                     /locus_tag="RD521_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771496.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(13466..13843)
                     /locus_tag="RD521_001784"
     CDS             complement(13466..13843)
                     /locus_tag="RD521_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7771497.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(13864..14178)
                     /locus_tag="RD521_001785"
     CDS             complement(13864..14178)
                     /locus_tag="RD521_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7771498.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(14378..16171)
                     /locus_tag="RD521_001786"
     CDS             complement(14378..16171)
                     /locus_tag="RD521_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDT7771499.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            complement(16168..18357)
                     /locus_tag="RD521_001787"
     CDS             complement(16168..18357)
                     /locus_tag="RD521_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7771500.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(18491..19681)
                     /locus_tag="RD521_001788"
     CDS             complement(18491..19681)
                     /locus_tag="RD521_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDT7771501.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(20553..22184)
                     /locus_tag="RD521_001789"
     CDS             complement(20553..22184)
                     /locus_tag="RD521_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDT7771502.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22198..23871)
                     /locus_tag="RD521_001790"
     CDS             complement(22198..23871)
                     /locus_tag="RD521_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDT7771503.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(23884..24201)
                     /locus_tag="RD521_001791"
     CDS             complement(23884..24201)
                     /locus_tag="RD521_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDT7771504.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(24226..25626)
                     /gene="lacG"
                     /locus_tag="RD521_001792"
     CDS             complement(24226..25626)
                     /gene="lacG"
                     /locus_tag="RD521_001792"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDT7771505.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            25863..26648
                     /locus_tag="RD521_001793"
     CDS             25863..26648
                     /locus_tag="RD521_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7771506.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            26651..27343
                     /locus_tag="RD521_001794"
     CDS             26651..27343
                     /locus_tag="RD521_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7771507.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(27617..>27888)
                     /locus_tag="RD521_001795"
     CDS             complement(27617..>27888)
                     /locus_tag="RD521_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDT7771508.1"
                     /translation="SSGISDYIDMSGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDT
                     YLVGKLINILGTEKDTETFISSLSMLFTDKHFSTQLGGEELVEIF"
BASE COUNT         8402 a         5607 c         4624 g         9255 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aaacccaaaa gggcataaaa atcccagtga taaatacttt tatcactggg atttttatgc
      121 ataaccatag gtatacaaag catacagata ttctctggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tctaattatt
      241 tgttcccgct atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gtcacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa aaagagtaca tgattacaaa gtatctgtaa tcatgtacca atatttgtta
      421 ttttacaatc ttccaattat cttgtttttc taatatcaac tcaaattgcg agatttgggt
      481 tgcctttgtt tcttgatcta aatatttcac agcgacattc acgattactt ggttatcttt
      541 tctggtaaag ataggattca ccaattcctc gaaaacatat tccttgttaa tcataggtaa
      601 cacatgatta cttacatagt aagtcaattc tttttctgtg gcggtaggat agagctggaa
      661 gaacgtttct aaaaagctag tgatttcgtc catcgtttct gtatctactg tatggtcact
      721 ttctagctgt ttgggttggt agtccgattt ttgaggtttc ttactcattg tcggattttt
      781 gataataacc atattatctg attcatctat atggacaacc acatgaaagc tagacgatat
      841 agtttccttg tctttgtctt cggtaataac ttgttcaaca gaaaacagta cctcaaaggt
      901 gttctcattc acttgagaaa cctgccacac ctgtatatcg ttcacactag aactagtagg
      961 aatatcctta cgaatcattt cttcatttaa tacctgcaat tcttccgtca aataatgggt
     1021 caatttttca tttcgtttat caatggcttc ttgggattgt tgccacgaaa agtagtcttt
     1081 cgcaaaggat ttcacaaaac tttctacttg gttggtatcg acaatctgct gtttaatgat
     1141 ttctgtttca cggacagtat gagtgtcaat ggcagtaaaa tttttataga ttccaaatcc
     1201 gacactacca atcaataaaa cccataaaac aaaggttaat ttcttatgcg tgccaacttt
     1261 aatcatcgga acttttcttt gcttgctcgg ttttttagat ttctttggtt tctccttctt
     1321 ttcttttcgc tcgattttta taatcatttg attcattcct ttcttagtta tgaataacac
     1381 gtctagcccc aatcagatgg tcttgccaat aacgactgct taagtcgcca taaccgattg
     1441 ggtcgcctgc atgataaaag cgatttccct ctaaatatat cgccacatga gtcacatatg
     1501 tgcctgcatt ataggttgaa tgaaagaaga taaggtcgcc tgcttgggct tcttccatag
     1561 agatttcttg cgtagcgtca tactgatctt gggcgacacg agggagagaa atgccagctt
     1621 tgtcatacac ccattgtatt aagccactac aatcaaaaga ggtggtaggc gacgcccctc
     1681 caaagacata gggaaaccct tcgtatttca aggcttcgtc cataatgact tgcacggttt
     1741 catcatcaaa ttctgttggg gaagtgtcgg ttaggtattg tgaaaccaac tgaacataaa
     1801 actgatttcc atagttatat cgccaaccac cattcacagg tatggctata ggattgggat
     1861 agtctgcttt ttgaccaccc gacttctctt tagaaaactg ttcggctaat tcataggtat
     1921 attttttccc atgactcctc acataattta aaaagccacc gccataatta taagattgga
     1981 taacagaatc tatatccact ccttgttgtt ctgcactggt taataattca ctgaaatatt
     2041 taaccccttg tttgatggac tcttcggtac tcaaactgtt gggcggtaaa cccaaagatt
     2101 cggaactttg cataacatct tctgctgtac cgcccgattc cacttgcata atggctaaga
     2161 tataagaaac atagtcttca atcccatatt ccttaccata tttttcaatc aacggacggt
     2221 gtgccaacac ttctggtgaa acggtcacac cgccttgtgg aatagaagaa tccttgttgt
     2281 tttcgccact gtccgtatca tctgcaaaga aaatgactag gaataataag agaaaaaaga
     2341 agagagagaa gaccacacta caaatcactg ttatttttag cttcatgttt gttgtcctcg
     2401 cttatccgtt cttttattta gcttcctctg attgaattgc ttcggtggtg tcttttgttt
     2461 ttgctctttt ttcaatggtc gctgatgatt ttcacgttta gtgttttgtg acaaaacagg
     2521 cgtgacggtt tgctgacgct tgcgaacatg ctgctggtta ttctgaacag gagatgatgg
     2581 gttttctttt cgtctttttt cttttgcttt atccagctcc aaacgctttt gagcaatgtt
     2641 ctttcgtttt tgctcctgct ggttcatacg ctgttgtttg cgttgagctt tggtatctgc
     2701 catagattgt ttaaagtctg ttacattatg agtgacattc tcttttcctt gatgaagggc
     2761 atatttgaca tgggttggtg tcgcctgtat ctgttgtttt gcctgctttc ttttattgac
     2821 tagacgattt ttcgtgtcca atacatgggc tgtcttttca cctaatcgtt gcccgatacc
     2881 gccttgttgc tttttcacat tggaaagtgc tgtttctttc tttggtgtag tccgttcatt
     2941 ttgacgagta tggttagctt tattggtttg attctgcttt gttgtcattg tcttttgctt
     3001 cgttgaccct gcttttccac gaaaaatctt actcatactg cgttgaaatc ttctggtttg
     3061 tcgattcatc atttgacgag gtctgcgaaa cacttgtctg cccatgtttt gagagtcgtt
     3121 gctttgtaaa gtgaataggc tcattaaatc gcccagttta aaataaatcc ctgcaaaggt
     3181 cacaatttgt aaaaacataa tcatgaaaaa gggagaactt gctgacagcg aataaagcat
     3241 agtggaaata ctaaaggcaa cggtaataat caaggtaata ccagcccttg tcataatcac
     3301 attaaataac ttggtaatgg catgcctgcc catgccatca aaactcggca acatacttaa
     3361 taggaaactg attggcaaaa acatagcgaa aataataaat agcacttgcg aaaaaatcat
     3421 gattcctgtt agaaggaaaa caaaaatcga gataccaata ttgaatacaa acaaaaagaa
     3481 gactgttcct aatctggaaa tcgtcttcgg tagggttaaa taggtattgt catgatcttc
     3541 aatttcttca atcactacat tttctctatc ttcaccattg tttgtatctg gactggtaga
     3601 caataaactt tccacacgtt cttcccctaa cgcttctaca tcagtggagc catattgaag
     3661 caatagccat ggttgcttta cttgaacaga gaataagctg tcacgtatca tgtccacact
     3721 gtctttccct tgactatccg tgttaggaag aacaattttt gtcccaatat tcagactggc
     3781 ggtactgaca tctttggaaa aatcattgat tttcgcaata taatcgggtg catacgcaat
     3841 aaaggaagcc gatagaataa acaccacgac aaagtttaaa acagctcgta tcactttcgt
     3901 cgtttctcgc ttaaccaatc ctgtataggc aacatagatt ccaacaatga gaataaaaag
     3961 aaggaggaaa ccgacataaa accctgaact ggaaaagcca ttcggtgtaa tgcctgcaat
     4021 cgtctgcatg ttttcgccaa ttgcttggga tgtttgacta atgaaatcta aggcataggc
     4081 ttctttcacc aaataacctg tggcgttcga tagataaaga gaaattgtcc aaataaaatt
     4141 agtaatggca tacaatccat acatcaccga ttgtcctatg ccatcactcc aattccatgg
     4201 aagccagccc cagctattgt ccacataata atcaagctga tattgattca aaccatactt
     4261 ggagtattca ttgcttgtat caatggtttc atcaactaag cctgtcgctt gtactgcatt
     4321 gcccagcgtg cctaataaaa gtaaaataac gcccacacaa aggagcgtta tccaactata
     4381 gtgaagaaat ttcttttgtt tttgtgtcat tcttccacct catttcgcac aggcggtctg
     4441 gtatcaaagg catgaagcaa ctcttcaaaa atcgggtgaa actgcataac ccctacacga
     4501 ccatagatgt ctgaaactaa acattgtcca ttttctaatt cacgtaagcg tttctgattg
     4561 ttttcatctt ccttgtccac accaaaaaat tctaaggtct ttttgatttc cactaggtca
     4621 gtggaacgaa aggcaaattt caagccaata ttattcttca tcttttcatc tagtaaatcg
     4681 tccgcgtttt gagtaacgaa atagacccct gcattcatgg cacgtcctgc tcgaaccaat
     4741 cgcatagata gagcttttcc ttgtgccact tgtaaaaaac tccaagcctc atcaaggtca
     4801 acaattttaa aaatggaacg gtcagagtgg ataaagtcta aagcaaaggt actaatgaca
     4861 atcagcattg ccacacttaa taattccatg gtagtgtatt cttcaaaaga gctattggaa
     4921 tcgggtaaga ccaaatctgc cacttgaata atattgagtt gtttttccaa gctaatggat
     4981 tgggtgacac tgccatcact gaataacaga tgggcaaaat cataatcgac aaagctttcg
     5041 atatggtcag caatactatt gctgatgggc gtatcttctt ttcgcaattc atcaatcacc
     5101 aataacaacc ctcgttgttt gctttgagtt acactccgaa tagctttacg caggacagga
     5161 aacttctcgc tatctcggct ggaaatacct gttaagaacg tgagaatatc aatggcaagg
     5221 ctttctgaat ctttcgggag ttttaaaatc acataagggt caagtaaccc tttattgtcg
     5281 ttctcactcg tgagattaac aatattgatt tcatgggcaa tatcaggtaa ggtttctttc
     5341 cattcaccac gttcagcttt tgggtcaaca atggttgcct gtccgccaaa gagtactgaa
     5401 taatagacca gcatattgtt agagaatgat tttccgccac ctaatgaacc aataaaagcg
     5461 gacgctaatg catttgttac agagccttta attccttgac ttgcaaggct aggttggaga
     5521 taaatatttc tgcctgtatc gagattgtaa ccgacgtaaa taccagaatt ttcaccaatc
     5581 atttgtgtag caccgaaacc aagaccagcc aaaaaatccg acgtgacata ttgaatatag
     5641 tcattgatat atcgcttgct tgagggaata aattcattat gcaagcctag catatcgcca
     5701 aaaggtcgta ctaatttgat agacagatca tcataaaaat cttttacttc atcacaacgt
     5761 tgtttcagct cgtccaaatt ctttgctgat acccgtacca catagcttag tttgtacata
     5821 gattctttgg tttggtctaa attactttct aattcatcga cagattctaa agcgtccacc
     5881 acattagtac tggtttcgtt gtcgctttcc catgcgtggc tatctaaatc tttcagttct
     5941 ttctttttat tgcgaacggt agataaggct tttttattgg cgacaatttc aacattcata
     6001 ctggtatcaa cggggaaagt aaactgctgt tgctggtaat aaaaaatttc actattaggg
     6061 aaatccaatt caccaacaat actattaatt gtaaaatacg ccacataagt agtggtatct
     6121 tcctgttcaa tccgtaaata gcgttgattt tcttcgatta aacagcgagt gggtttgaga
     6181 atatcgtatt tctttaccaa cactttttta tcttcgtatc gtttcggtaa atggtagtcg
     6241 tagtcatcaa aagggacacc tgtttgcccg taaagatgtt caataatata accaaaatca
     6301 tctttctcca atttacgaat ctgaaagcgt ctggataatt tactttctaa caactgttcc
     6361 atcttagaaa agcgttcgat ttccgccata ggcatagaaa caaaatcgcc cattaatttg
     6421 tgattcacac catgaaagaa ttccgcaaat gctttttgaa tttctttccc aacatttttt
     6481 acggatactt cctgttcatt gaggtagagt ttaaaaccaa taaaaaagcg atagtctact
     6541 tgattgtcac caatcataga aattaacgct tcggtctgtt catctatttt ttcgtaagct
     6601 atctctttta atcgtccagt gatttctttt ttcgattgtt cctgtgtggc tcgaatactt
     6661 gattcggttg ctagttgcag agcatgaatc ttgccatcac gattttgggc aatcaattga
     6721 cagaaattat catgaacttg aattttttca tcggaactta aaaaactgta attataggga
     6781 attaattcat aataagcgaa tacttcgcca tcatgattaa atacgagatt atcttccata
     6841 tatttaatcg gatatgccat ctagttgttc actccttaca atcgtaatcg attcattcat
     6901 tgtttctttt tttaatcgta tttgtttgcc agcgtaggtt actttagggc gtagagcata
     6961 ggtaatgata gactttagaa atccataggg tttctttccg tcaaaggtct tttggcacat
     7021 gaacccagtg agaacaatgg gaatccctaa gtatttgagt agtgctccgt caatcagaga
     7081 aagtggtggt aaactgccaa agagtatgac gacaaacaaa gaaacgataa accatgtcat
     7141 ttgcgtaaag gtaattggga aagggagttg taaatcatta atcgcataaa tcactttttc
     7201 tactgaccaa atactggtgt aacttcttat ttttttcatg tcttcacgtc ctttccgtaa
     7261 aaatagaaaa agaacaatca aaataggtga ttgctctttc aatgctttaa ctattcaatt
     7321 ttattttttg tccattttgc aataatacct tcatcagttt cttcatcatc tggattcatt
     7381 gaatatctga aacttaaatt ggtatctgac cctaatcgtt catataagct gggtctatac
     7441 aatctcgttt tattgtcatt aaaaggactt cgttttccca gctcaccatt tccctttaaa
     7501 ggatttcttc taactgaaaa acaatatttt tttctgggat cttctgaatc actttgactt
     7561 aatgagaaac acatggcttc tttttggtct tcatttttct tctcactaac ttctgtcgga
     7621 ataaatcgag caagagtttg gctaaattga aataaaacat ctcccaagtt actactgtat
     7681 tcgataccaa aatattctct tattgttttc gtgtctgtat ataattttac cgaattagca
     7741 tagacagcta gcatatctga aaaatcgttt tcctttaaaa attctagttt taacagagca
     7801 tattctggaa ctttttcctc tttttcaaat agcttaacaa gtacaatata attttgctgt
     7861 ttgtaacgaa agtaaaagga atctatcacc cagcctttat ctttcatgtc tagttttaat
     7921 agtttaagat tttcaaatac atttaccata atttatacct ctccgtgcta tttattaatt
     7981 taattataac acggattgtt aaacttactg acaatattca aataccccat gagaggtaac
     8041 taggtaatta ccctctattt ccatatcacg tcccaaagat tggtagtcaa tataattttg
     8101 taaattggaa gggacttcac caagctgtcc tgtttcttcc acgtaataac gggcgacatc
     8161 ttccatcgaa tcacaatcac tataacaatg aatatcctct ttgttttcca gtaattcttc
     8221 caagctacta aaccacatgc cttgaatttc tttgagttca ttataaatag gtgtaccttc
     8281 gatttcttga atggcttcac atagacggtt gatttcacta atcggcgtgt attcgtccac
     8341 atcaaaagga agctcaaaat catgtattgc atattcttcg tagtcctcat ttaaaccgat
     8401 tctttctgcc atttcttcct catcaattgg tggagtaaac catgctccga ctaattcacc
     8461 ttcattatac cgtcctaaat ttgccacata gactttcatt tccaattctt atcacatcct
     8521 ttttataaaa atagtcataa aaaaagtagc aatcagttaa tgacgctact tatcaaggta
     8581 tccgctaata taatctgtaa ctgtacttga aatacctatt attagttttg cttctctctt
     8641 tccaataata tttgtattag ggtgtgcaag tgaatgccta tttcttgctt cattaagaga
     8701 gctaatcatt ccatttgatg accttatcgt tgtcttcact atatcagcaa gttcagtagg
     8761 ttgaatttct ttttcaatta attgttgtat cctactatat aatttagata gattttcgtc
     8821 tctaggaact gtaatttcat atttttttag tatttcaatt aaatatccat gaaaagcagt
     8881 gtgaactctg tcaaaggcac gttgatattc accattactc ataaaagact cagcgtcttc
     8941 aatagcttgt gaaaccacat cactaatttt caactgacta aagttaatta cttcgtaatt
     9001 ctctggaagt ggtactaaaa tagtatccac taatacatta tcaccttgtt tatcatatat
     9061 ggagcttgct gtctgggtaa tcaaatcttt atgactttca aaaaagtcta aatcatcaaa
     9121 aggaactctt attttaagat atgtgcaaag cgtattccag ccctttttat atcctttata
     9181 ataagtaaaa tcgtcagtct catctaaagt tacctctgat attttcattt tagaaacaat
     9241 ctcgttacct ccttcataat aatttgaaga gctctttctt tctaatgttt taattaaaag
     9301 agatttaaat tgatcatttg taaacatatt cataaatcct ttcataacaa aatatatact
     9361 tcctatttta tcatgaaaaa tatctgttaa gcaccaatga ttcggttaaa taagtttaat
     9421 aacacatctt tcacgccagc tgcattgaac acaagtccga cggcgattag agcaataatc
     9481 aagaagccaa tgagttttga aaattctcgt ttaaaaccta aaaacagtcc aatgacaaca
     9541 atcgctaaca ataccaatga ctgtgcatta tccaagaacc agttgtacaa attctgccca
     9601 aaattcatgt gattgtcctc ctttccttaa gatatttttc tcgataatag atatagcttt
     9661 ttacacgcct taataattga aaaggggtta aatgctctcc atagacggtt actcgattgg
     9721 tataattatt ataaaatctc accttgtaaa aatcataggt atagatggta tcaccagctt
     9781 gccgttttcc ctcaataatg agagacgtat atttccctgt attttgtata tacgcttgaa
     9841 actcaatgcc acactgaaac cctaacttct ttaattcttt tgttaaaagg ctcatgtaat
     9901 caaatcctcc atggctagat gttgttgttc aattaatttc tcatgttttt ctgtcagttt
     9961 ggcttcctga atcatggtgg agataatggt tgtaccgttg agaacatcta aaatgcttgc
    10021 gatttttaat gtaggggcga cttgatgatg taaccagttc aatgttcgtt caaaagaata
    10081 aggctctggc tgtgtggtta ggcgtaatgc tccacgattt ttaccgataa accattccca
    10141 acgttcatta gttttccaat cactccgacg tttagtgtcg tctttatctg caaagcggat
    10201 atagcgattg ataatttcaa aggctgtttt ttccgcctgt tgatgtgcca ataaatcttt
    10261 catggcttgg gtggctcggt cattttttaa ccgaatttca aaccgattct ttacttctgc
    10321 gtcttctaag ggaacatcat ttttcacgta ttgctcataa tctttctcat atatacagaa
    10381 atagacttca ctttttaatg agccaatata aagtgtgttt cccatgtatg gtttttcatc
    10441 acggtgaacc aattcaccac tgcgataatt tttaaagctt cgaaagacgg aaatgcattc
    10501 ttcctgttta catttctttg ccaaatctgg aatattcaaa atccctgttt tatcgttgat
    10561 agctaaatcc aagcgtttga aaataccttt atgttccatg caatcttgaa agaaatcata
    10621 ccagctccga tgttgagcca atagaaagtt ttcaaattgc cgacagcctt ttcctttcag
    10681 ttctaataaa acacctttac ttacatcatg agagactagg acaacaatat caccgaaacg
    10741 atagtgttcg ggataagaat aatagccaaa gtcttcgtgt agcatataat ccatattcaa
    10801 acgtaaaacc tcaccgataa tttgagccac atcattggta gggaatcgaa tccgtacata
    10861 gtcgaataac atctccaatg ggttatcggg attgaagcgt tccaaagcgt tcagtaaatc
    10921 ttctttcacc tgttgagtag ggactgcctt gcctgtttca atatccgata ggtaaggtct
    10981 ggtaatatga gaagcaaccg ccagtttatt ttgagaaacg ccataagcca gtcgcttttc
    11041 tttcaattcc ttaatccaaa ttttttcgtt ttccattcgc aaacctccaa tctaaaaaag
    11101 atgtaaacta tgacccttga gtttacacct ttcatatttt ccgcaatcct ttgtaccatc
    11161 aacattggtt gatgagaatt gcctgttttc agttgtattt gtgcccccct gttagataga
    11221 gggggctaaa ggattggcgt ggctaacgcc acaccaatca aagcaagtct gaacctgtac
    11281 ctttcacttc gtgcggtaca ggctcagatt gctttttttg aatgactttt cctatttctc
    11341 ctaagaaatc gtaccttttt ggtacaaggg gtgtataaaa ctcactaatc acactttttc
    11401 ctgtatccac gtaacctctt cccttgatgg gttttaagaa aaattgtttt tgtgtatcgc
    11461 ttccgaacat cataccaaag cccatttcag acatacgacc caaagccaca cggaagttaa
    11521 agttgtcccg aatcccatca ctaaaatatt ttgcgtctgg acgttgacag gcaagaatta
    11581 aaaagaagcc tgcttgtcgt cctaacatta caatttgttt gagtttatga ataacggtgg
    11641 tactttcacg acctagcata tccatcattg ccacatattc atcaaaaata agaaaattgg
    11701 cagacaaacc aagataagca taattttctc ctgttttata gttagggtgt tgtttcatgg
    11761 tttcactgcg atcaatcata tcttcgtaaa attgattgat acatgccaac atatcttctt
    11821 tacggtaata gacattattc atgaccgccc ctaagtctgc caaatcagca tttttagggt
    11881 caagaatata aagctttgca tttgtatgca ggagagattc aatcaatgtg agaataaaat
    11941 aggattttcc actaccagtg cccccagcaa ttaacatgtg agggagagaa tcgaactccc
    12001 aatagaccga ttccatcaat ttgacacgtc catcttgagc taccacgtct tcaatgccaa
    12061 ttcgattgcc aatagtatca taaagtagga catactctac ataagaatct ttcaacgttt
    12121 tttccactaa ttcacaatac aatcctgttt ctagtttgtt ttctaggtgc aaaagctgat
    12181 cttgatattt ccctaaggtg atttctacat gaatatggat tagtccatct ttcattcgat
    12241 aatatatttt aggaaaatgg cggattcgtt gtttggtttt cttgctgggt aaatctttaa
    12301 agaatccctc tgattgagtt tcttcggctt cataccaatg attttctaaa atcatacgag
    12361 ctaacttttg acggtgctct aactgtttta tagaatctat acgatagtga taataaagcc
    12421 agctgataag tccgcataca aaaagagcag tgagaatact gctccaaaag tatggcgtat
    12481 gaagttgata gaaaaatgta tctaactgaa ctgcttgcca gttcaccgat tgtaacgctc
    12541 tccaatgaaa agggagtagg acgactagaa aaactaccag caatgaaatt agtacaaaat
    12601 gatagaccaa gtgcttatcg ttggctttga ttcgctctcc tctgtcatga agcaatggca
    12661 taaaaatcct cctttccacc tatcaaaata attgtgtatt gtagatacga gcctgtttat
    12721 caaaagttgg ttgagaaata gcttcatatt tctgataagc gattcgcttt tcaaagcgta
    12781 aatttttttc tttcggataa taatgacgtc gatagagagt gtgtcctaac agttttttcg
    12841 cttcttttgt caacggatag agatagcgaa acatgcgtcc attgattttc tcaatccctt
    12901 tgtattcgca aaatgcctgg gaaagccaat gctttttctc tacctgttca aagcgagcat
    12961 tttcctctaa taaaaggcga gcacttcgag ggtgtatctt ttcccatgac tgcttatcac
    13021 gatagacact tgtcttgaaa tacccgcagt aaaagaagtt ggacgcttga tagacatagc
    13081 cacattttcc ctcaattcca tctgccagtg tatataaaaa atgacagtcc gtatgttctt
    13141 tcaaccactt aattagagct gaaagagctt gactgccaaa ataattggtt tgattcattt
    13201 ctggaagaaa acacatcttt ccaatttcta aataatctgt ggtttgaagg gaactgtcgg
    13261 gaaagagttt gcgtatggtt tgtaggggtt gcgtacccca gcctaattcc acaacgccta
    13321 ataatttttc ttctgaaaaa atccctagaa agtatttaca caatctaggg agtatctttg
    13381 aataatggta ttgccgaata aaagaaatgg cttcatcctt cggaatttct ttcaaaatca
    13441 atggtgtgtt tgtcataagc aacacctatt tttcactttc ggacgcttct tttttatatt
    13501 tctgatgagg ttttgtctgt ccttttaata caagatcgtc tgctttcacg taccaatcaa
    13561 cgtctgcccc tcgaaaagta gccgtcgcta cggtatctgc cacaggattg attaattcca
    13621 cttctgcgtt gtagtcaaat tctttcaaag gaacagaagc ggggatactg acttgtatca
    13681 tgcgtccttg tccttttgat tttaagtcat aggtacgttc tttaatttct tcggaaaccg
    13741 taccatcttc attctggata cgaacctcac gacgcaaggc ggaaaatttc aatgtgccaa
    13801 atgtctggtc tttttcaatc acaatacctt ctgctaatct catatttttt cacctttcct
    13861 ttcttatgct ttcaccatat cgtctgcgtg cataatgtaa ttggtaaatc ctcgattgcc
    13921 aattttataa ccttctgcgg taatatgtgg gttgatgagt ttgacttttt cgtcggattc
    13981 aaaatgtttc tcgccagctt cggagggaag aatgacaaca atatcatctg ctcgttgaat
    14041 cgttgagtat aaattgtaac tacgagagag aatggttggt ctgccattta ttcgacgttg
    14101 ttcaatgttc ccttcaccag catattctaa ttgtccaaat gttttttcca tattgggaat
    14161 gacatattta agttccatat ttttacctat cctttctgtt tttatgtttg aatgaatctt
    14221 atttgtgtga tggttttaaa ggggggagag cgaccttttg attcttgatt ttttattttc
    14281 ataagttcac ttcctttcaa aattgggtaa aaaaatagac gctccgtact gagcgtccga
    14341 ttcctactat atttaaaaca acgactacct aggtatttta aatattataa tcaaattcta
    14401 ttttatctaa taattgagtg ggataaagca agtctccttt actcgtaaaa taacccacct
    14461 ctgagaaaag aaataaatta ttttttgctc ttgaacaaat tacgtatagt agttttctcg
    14521 ctgaactaat tcctttatca gaaacattaa aaatttcatt ccaatgagga attttcccgt
    14581 ttagaagatt aatagcgata acagtatcat actcatttcc tttaacttta tgtgctgtag
    14641 taagtgtgat accatctcga gatttaaaca tcttcttaaa actagataca ctactagata
    14701 atgaattatt ttttatccga tcttctactt cgttaataaa aaatagatag tgcttaaaaa
    14761 tagacgggta atttttctcg tcaatattaa gattaacaaa tatatgtttg gtaacaaatt
    14821 tgtataaatc aacaccgttg tcgtctccac tatacattct tttcacactg tttattagct
    14881 gtaataaatc taagtcaata aaataatctt taacttctat tccaaattca ttttttaata
    14941 taattaaaat atcgtttgca attagctttc ttcttcgaac cttttcacct gattcagtaa
    15001 agtacaaaat tgctaacttg aaaaataaac tatgattatt taccttaatt ggtgatatat
    15061 ttatagcatc aaaattgcaa tacggtaatt ctttctttaa atactcaccc agtgaaaaca
    15121 gaggatagtg ttgaggagca agtacacaaa tatctttttc atgaatgccg ctttcaatct
    15181 tttcctttat tatatttttt attatgttgg gtaaatcctc cttagtaact ttgcaatcat
    15241 aacttataaa accacggtcg tttttattat tccctctgct ttcaatctcg taaggacaac
    15301 tctgaaaatt agagtagaag tctactaatc tctgattaga cctgtaacag ccatgtagcg
    15361 tctttgattg aaaagtgtga ctagttagca tttctagttc ttcaactgat ttagctatac
    15421 catctaatga tccataaatt gcttggtcaa tatctccaac aaacattgac tgaatttgag
    15481 ggttgttttg aagtaattta gctattacat tgtattgtaa ttctctagta tcttgaaatt
    15541 catccacata aatcgccctt atattactag caatattacg tgcaacgtta ctgtttacag
    15601 ttaaaatttt atacgctact agtaaaatca tatcaaaatc aatttcttta tttttttcca
    15661 acatagtgtg atacatttca gaaacttgag tatactgaac ttcattaatt ttaaaatcat
    15721 gagaaagtga caaatcaatt ttgttcagtt catacgtttt gagatttaaa ttcagcatat
    15781 tattaatttg gctgacgtaa gctctttgag tataatcatc aataattgta aatccttttg
    15841 atactcttga gagattcatt ttaaaaggat aaataataaa ttcaagacaa aactgatgta
    15901 ttgtccccac ccaaatatta atatcatcat ctatactaag taaacttagg cgttccttaa
    15961 tttcttcagc agctctatta gtgtatgtga tagcaattat tctttttagt gatccatcgt
    16021 gtttaattgc ttggtaagca attttccttg taagtaccct tgttttccca ctcccaggac
    16081 atgcatttac ataaaggtta tcttcacttt ccacaacatc tttttgttct aaatttaaat
    16141 catttacgat tttttcgaag atatttgtca ttttgaaacc tcagcataaa gtatttttgt
    16201 gtaattttct atctgattga ttagatacac aacatcctca tctttaccaa agtactctaa
    16261 taaagaaatt attgctatat tcaaatcagc tgtatcactt gtttttaaac tagctgataa
    16321 agctgacaat tctgtagttt cctcgtacaa gtcgattgta tggagtagaa tttttttata
    16381 aattgagaga tctactattt cctgacatgc aaaagcaata gcattaagaa tatacttagg
    16441 aattttgatt aaaaagtcaa ttttttcacc taactcaatt gcaaaccacc ccttaccaac
    16501 atgattggct agttttaaca tcgctaaatt tctttctaaa tattgttcac tttgtatttc
    16561 ttctttataa gcagttattg tttttttatc tttgaatgcg tcatctataa ttggactgac
    16621 ataagttaga atattttctt tattcacatc taaaaactca atttcaaatg ttgtattagc
    16681 ataaaaacaa ttaacccaat tgttttcatt aaaaagggag gataattttt cttttcttgc
    16741 ttgacctttc tcttgtgcag tgcttttata caatcgatgt tctgatggga ttgcctgttc
    16801 atctaaatca gttacaatag cacactttct ctgtattcta tcatcgtcaa acaagctagc
    16861 gatatattca aacgaagtac taccaacatt aatcaaacct attccaagtt cgtctaagtt
    16921 tataccaaat aattttttta ctatttgagg aataagtatt tcttcagcat ctccttcaac
    16981 taagataaca cctttagaaa aaagcaatcc atttctttta acatctaaat atctctctac
    17041 tgcgtttatt agatttagat tgttcttttt taacttttct ttagcaaatg agttcagatt
    17101 atttgctgga ttcataacga tagatttgcc attaaacgat tttaatacat tcatccgaga
    17161 tatttcagac acttcagcta aatgtacaga atgtgtggtc atgagaatct gagtatagtt
    17221 tttttgaaga tttaatcctt cgaacagagt tttttgaata tgatgatgaa tatgagcttc
    17281 tggttcttca atcaacataa tattaagtag ttctcttgaa cgacacgctt caaattcgac
    17341 tattttcaaa gctaaataaa tcatatttag atgtcctaac cctagtaagt ctaagtcgat
    17401 attttgttct ggcttcattg aaagaaattt tgctaaagaa cccatatcat ctgacatagt
    17461 tgaggaaaga agaagattag gagaatacac tgaaccaatt atgctattta gttgccgatt
    17521 aatattttcg ccaattttcc taatttcagg aacactggta atagtctcat ttaattgctg
    17581 tataattgtt tttaatgact caacattatc tgatgatatt tttgattcaa ttgtttccat
    17641 gattcttttg actgggtttc gattgttttt catttctcta agaacatctc gaagagcgtc
    17701 aacataaaca accgaaatat gcttgaaaat atctgacatg tcaattcttg agcccaacag
    17761 agcttcatcg tcttcttcag gattactagc tatattgtca ttgagtcttc ctaccaattt
    17821 atcataattt tcattgatac agaaatttag atttgattta gatgtgtagt aaaattcata
    17881 gtcggataga cgaatagaat ttctaaattc gttaaattga tgagagtcta attcagtgga
    17941 aatatcaaat aattgctttc taatagcttt gttaggtcta ataaatagag aaatactacc
    18001 tacaccttta tctgcatttg aaatcaattc ttctaagatt gtttgacttt cagaatttaa
    18061 agaaatggaa gcacaaatct cattgtcaaa ttcttcttcg ctgattcctt caaatgtcgc
    18121 agagataata atccaatgcc cttgccaccc attttgaatt ccatgaaaaa aatctgattc
    18181 ttttagtgtt ttactactat agtaataact atcatctaat aagattctca atgcagtaag
    18241 agcattggtt ttccctgaat cattctcacc tataatagta tttgtctcat tatcgaatcg
    18301 aaagatttcg gattcataat ttctaaaatt tcgaatttga agagtagata taaacataag
    18361 cagatctcct aactatttaa ttaatatgat tatatagtaa gtttatcata aattatgtta
    18421 gtaaaagttc tcattataag ggagcgtcaa taattttgtg taaatgattc ttccctactg
    18481 cagattgttt ctaattctca atcagttggc ttagcatttg ttcgagctcg ccttcggcct
    18541 gttggaaacc tttatgactg cgacctagga atttttggtt gtaagtatca aaagacgaga
    18601 ctaagaaacg ttccatcgac tcttcatttt ggaactgttc cttgcggtgg ctgtatttct
    18661 tgatttgttt attaaaggat tcaatcaagt tcgtagaata aatactcttg cgtatggcca
    18721 aggggaaatc atagaaagtg agcaagtgat cgttcgaaag aatcgattca accacttttg
    18781 gatagctggt tttccatttt tccgcaaaag taccacgtgc ttccaacgcc acctctttag
    18841 atgaggcttg atacaccatt ttaaaatcat cacagacttc cttacgatca tcgacacgca
    18901 ctttatgact gatattacgg gaaacgtgta cacaacaatg ttgaaaacga gctttgggat
    18961 agaaccgact aatcgctcct accatcccct ttaagccatc cgtgatgaac aggaggacat
    19021 ttttcaaacc gcgctcttga aggtccaata aaatttcctc ccaaatcgtg atggattcag
    19081 tcggtgcaat cgcatagctc aatacttcct ttgatccgtc cgggcgaatg ccaactgcga
    19141 tatgaatagc ttccttggcg acggttttcc gctttaacgg aatatacgtt gcgtccatat
    19201 aaatagcagc ataacggtca tgaagctccc gcccattaaa cgccgttacc tcttctgtaa
    19261 atgattttgt tatattggac atggtttggg gcgtgtagta atgcccatac attttctcaa
    19321 tcaagtctgc gatttccgac atggtaatac cttttcggaa gaggtgaatg acggtctcct
    19381 ctaacgtgtc attcgtccgt ctataagcag gaacagtctg ttgcttgaac tcaccattgc
    19441 ggtcgcgcgg aatctggaga tgaagttccc catactcggt cttgaccgta cggtcatagg
    19501 agccgttacg agaattaccc gtgttaaaac caatgcgatc atacttttcg taatctagga
    19561 aagccgttaa ctccgttttg agaagcgtat tgactgcttt ctccaaatgg acacggaata
    19621 attcattcaa atcgcctttg ttcgctagag tctttagaat ttctgtagta aaatcgttca
    19681 tagggaagtc ctctcttctg tgaatgtgtt gtggtaactc tattctacag aagggacttc
    19741 cttttttcta tttacacaaa atattttaca ctctccatta taacaattgg gttaaaaaga
    19801 aaatagatag tattctacct catatttatt tgaaagtaat atataaaaga cgttcaaatt
    19861 cgaacgcctc tcgttaacca aatattggat tcagaatcta gttttcatat ataaacagag
    19921 tatgattagg tgcaaaaatt gcacttttat tgcactttta tttttattta tagtgtttta
    19981 ctgttctgta tattgtttta actcccttga tagtatcgca ttctgtttta tacactttac
    20041 gttgaaaaca gagtgggaat aaagagccaa cataatatgg agggggaatc cctataaatc
    20101 aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt tccctaccaa
    20161 aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag ttctcgaaaa
    20221 acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca tattcacgac
    20281 ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa ctttttgcaa
    20341 ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca aagtgtagat
    20401 gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct ggttattttt
    20461 ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg gctgaaggga
    20521 acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga aacaaaatca
    20581 acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca aacacctgtt
    20641 gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac aaccgcagta
    20701 tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa ctctccccca
    20761 gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac attttgataa
    20821 gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc aaaaaaagtg
    20881 cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt ttgccaattg
    20941 gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag atttatttgc
    21001 gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa atcaaacatc
    21061 gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt catacctgct
    21121 tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa gaagcccgcc
    21181 ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata ctctgcttgt
    21241 cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa agcatttggc
    21301 atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat cacttcttta
    21361 ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga accgtgccaa
    21421 tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt gatttcatca
    21481 aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat tactggagtt
    21541 gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc agccaatcga
    21601 tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat tccgctattc
    21661 agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat ttccgacaga
    21721 acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag accttgccat
    21781 gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg aaaatccagc
    21841 agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt ttccagatca
    21901 tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca caaacgcagt
    21961 cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga aatatcgtct
    22021 tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc tgctccgcga
    22081 ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc ccctaacaca
    22141 gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc tcctccttta
    22201 ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta atccgatata
    22261 ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt ctttgatatc
    22321 atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct gcagcatttc
    22381 gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt cagatgcacc
    22441 tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa gtaccaacac
    22501 attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta cttcctcacg
    22561 tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg cttctttctc
    22621 tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt caacgacgat
    22681 tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa ccaaaccgat
    22741 cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca atacatcgac
    22801 aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa taaaaaaata
    22861 agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga aacttactgg
    22921 aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga gtaggaagat
    22981 aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat tccctaaacc
    23041 caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat tggtttccac
    23101 attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat gaacaccgac
    23161 aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc cgtcagctgc
    23221 tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt ggaagatttg
    23281 tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag gaaaaatatc
    23341 tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg taatatcttt
    23401 cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg aagccagtag
    23461 tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga cgcttaacaa
    23521 caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt tgtttttcgg
    23581 taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa cgagtaaccc
    23641 tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag attcaggcca
    23701 ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg aaaataaaat
    23761 agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat tacgtgagat
    23821 tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca tagcgttctt
    23881 ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat ctctcagcaa
    23941 taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct ccatctcttt
    24001 cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg ttaaattttc
    24061 gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta cttctttcaa
    24121 taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa aaccaagcat
    24181 ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa taatcgtttt
    24241 ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct tctgggtttc
    24301 aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg agaaaagatc
    24361 cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg ccttgcttaa
    24421 ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa tgccatcttc
    24481 aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt aatgtgggta
    24541 atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca gccagtccca
    24601 atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca ctttataaac
    24661 agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat cgtaagcttt
    24721 gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg atgctttttt
    24781 caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt cacatatctc
    24841 atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat ctaataagaa
    24901 acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg cgtctgttgc
    24961 cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt cgttttcttt
    25021 gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg cgacgatctt
    25081 tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat tggttgcaac
    25141 tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat gaaaacaaaa
    25201 aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc catggtcaaa
    25261 caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt ttcttttctt
    25321 acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt tcccagctcc
    25381 atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac caaaacgttc
    25441 acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat cgggactgaa
    25501 tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac cttccttagt
    25561 ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt ctggtaattt
    25621 aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa ctgattgcat
    25681 tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat aaaaaccaga
    25741 aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt tacttttgtt
    25801 cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta gaggtgaaga
    25861 caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa aaaggaatcc
    25921 ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt cgcagagatt
    25981 tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct caaagtcttt
    26041 cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag attcaagaaa
    26101 aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg attttcttag
    26161 gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa attcggatca
    26221 ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca gaaattctac
    26281 tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc accaacgaca
    26341 atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt cacaacgaag
    26401 cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta aataattccc
    26461 gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt tatgtgtttt
    26521 ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg gaggttcaag
    26581 agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat accactatag
    26641 gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt tggactctca
    26701 tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg gtttatcaga
    26761 cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga aacatttcat
    26821 ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag aaagctatta
    26881 tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc ttgaagcgac
    26941 tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta ctgtcaaaga
    27001 gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact gggacaagat
    27061 tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc ttaaagcggc
    27121 agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt actcaggatt
    27181 acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat tcggtaaaaa
    27241 caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag attttactgg
    27301 tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc aactaactac
    27361 caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt gaaacgacat
    27421 gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac tttttcattc
    27481 ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata gtgttttcac
    27541 attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat acttttatta
    27601 atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc tgagttgaaa
    27661 aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta tctttctctg
    27721 tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta agatctgcct
    27781 tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct ttatcgccat
    27841 aattgatttc accactcata tcaatatagt cactaatccc tgaagaaa
//
LOCUS       DAPBCS010000035        27860 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPBCS010000035 DAPBCS010000000
VERSION     DAPBCS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.035"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            38..1405
                     /gene="nhaC"
                     /locus_tag="RD521_001796"
     CDS             38..1405
                     /gene="nhaC"
                     /locus_tag="RD521_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDT7771509.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1686..2705)
                     /locus_tag="RD521_001797"
     CDS             complement(1686..2705)
                     /locus_tag="RD521_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771510.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(2727..3734)
                     /locus_tag="RD521_001798"
     CDS             complement(2727..3734)
                     /locus_tag="RD521_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7771511.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(3727..4392)
                     /gene="pgmB"
                     /locus_tag="RD521_001799"
     CDS             complement(3727..4392)
                     /gene="pgmB"
                     /locus_tag="RD521_001799"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDT7771512.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQTGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(4385..6679)
                     /locus_tag="RD521_001800"
     CDS             complement(4385..6679)
                     /locus_tag="RD521_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDT7771513.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6939..9104
                     /locus_tag="RD521_001801"
     CDS             6939..9104
                     /locus_tag="RD521_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7771514.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            9408..10241
                     /locus_tag="RD521_001802"
     CDS             9408..10241
                     /locus_tag="RD521_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDT7771515.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(10343..12043)
                     /locus_tag="RD521_001803"
     CDS             complement(10343..12043)
                     /locus_tag="RD521_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771516.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            12550..13710
                     /locus_tag="RD521_001804"
     CDS             12550..13710
                     /locus_tag="RD521_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDT7771517.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13726..14169
                     /locus_tag="RD521_001805"
     CDS             13726..14169
                     /locus_tag="RD521_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771518.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(14652..15182)
                     /locus_tag="RD521_001806"
     CDS             complement(14652..15182)
                     /locus_tag="RD521_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDT7771519.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            15287..15838
                     /locus_tag="RD521_001807"
     CDS             15287..15838
                     /locus_tag="RD521_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7771520.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(15991..16464)
                     /locus_tag="RD521_001808"
     CDS             complement(15991..16464)
                     /locus_tag="RD521_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDT7771521.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            16602..18074
                     /locus_tag="RD521_001809"
     CDS             16602..18074
                     /locus_tag="RD521_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7771522.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            18071..18781
                     /locus_tag="RD521_001810"
     CDS             18071..18781
                     /locus_tag="RD521_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDT7771523.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            19133..19417
                     /locus_tag="RD521_001811"
     CDS             19133..19417
                     /locus_tag="RD521_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDT7771524.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(19698..20117)
                     /locus_tag="RD521_001812"
     CDS             complement(19698..20117)
                     /locus_tag="RD521_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDT7771525.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(20119..20703)
                     /locus_tag="RD521_001813"
     CDS             complement(20119..20703)
                     /locus_tag="RD521_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDT7771526.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(20750..22225)
                     /locus_tag="RD521_001814"
     CDS             complement(20750..22225)
                     /locus_tag="RD521_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDT7771527.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            22406..22717
                     /locus_tag="RD521_001815"
     CDS             22406..22717
                     /locus_tag="RD521_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771528.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(22832..25168)
                     /locus_tag="RD521_001816"
     CDS             complement(22832..25168)
                     /locus_tag="RD521_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7771529.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            25310..25978
                     /locus_tag="RD521_001817"
     CDS             25310..25978
                     /locus_tag="RD521_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771530.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            25981..27024
                     /locus_tag="RD521_001818"
     CDS             25981..27024
                     /locus_tag="RD521_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7771531.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(27261..>27860)
                     /locus_tag="RD521_001819"
     CDS             complement(27261..>27860)
                     /locus_tag="RD521_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDT7771532.1"
                     /translation="ILTIPTMINYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDAN
                     EHSDTIHFARSFQAPPSSIEWLPTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLL
                     GPTTNLYQPVLDLCREAGFEPKISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKEN
                     TVILPISPTTESYLAFTRKVGEHSLASDTFWEYLNQEEN"
BASE COUNT         8470 a         5071 c         5711 g         8608 t
ORIGIN      
        1 atggcagggc cttttcattt gaaataggag atcgacgatg aataaaaata tttctttaaa
       61 agaaagtatc gctgtattga tcgtgttgtt ggcactccta ggagtactga tcatcggatt
      121 ccagctctcg ccacatatcc cgatccttgc agctttcatg tgtttgttgt tttatggcag
      181 atttaaaaag ttcacatggg atgagatcca tgacgggatc atcaaaggaa tcactccagg
      241 gatcattcca atcctgattt ttcttttgat tggtgtctta gttgcttcat ggattttatc
      301 tggaaccatc cctacgatta tggtttatgg atttaaactt gtttctgtgc gtttcttttt
      361 accgacagca tttcttgttt gtgcaattgt cggagtgact gtcggcagtt catttacaac
      421 gatttcgacg attggaattg cttttttggg gatcggtcat ttacttggat tcaatgatgc
      481 aatgactaca ggcgcagtcg tttccggtgc ctttttaggg aataactgct cgcctttatc
      541 agatacgacc aacttggcag cgggtattgg aggagttaat ctattcgaac atatcgctgg
      601 tatgcgttat acggatttcc cagcattcgt cctttcgttg attttttata cgatattagg
      661 acataacagt cattcagccg atctagctag tatcaatgaa atgatccaaa acatgaaagc
      721 gagcttttgg atctctcctt ggacattgat tccactagtt attttatttg ggggggcaat
      781 caaaaaaatt ccggctatcc ctacattact agcgggatcg acagctgctc tcgttctttc
      841 ttttatccat gagaaaagtt tgacggtcgg aaaagcagca gacatcttga tgaatggtta
      901 tgtcgcaaat acacctgatc caaagctaga tgaactgctt tctcgaggcg ggatcatgag
      961 tatgttaggt tctgctagtt tgattttact tgcattggca ttaggtgggt tactgatcaa
     1021 atatctgatc gttgatacca tcgttcaaga attaaaagaa aagatggacc gaccttctcg
     1081 tttagtcggg tttactgcct tgagctgtat cggaatcaat ctgatcgttg gagaacagta
     1141 tctgtccatc attcttccag gagaaacatt caagcgttct tttgatgacg ttggcttatc
     1201 aaaaacctat ttgacacgaa cactagcgga ttcaggtgcg accgtcaatt ctctcgtacc
     1261 gtggggagtc agcggcacat tcattatggg aacgatgaaa gttagcgcgt tgcattactt
     1321 gccattcgcc tttttctcga ttcttgcacc tgtctttaca atattaggcg gttttttatt
     1381 gaatcgtcga cgtgtaaaaa attgaaggaa ggaaaaaaga ggctgggaca gaagcgttta
     1441 actccaagaa ataagaagaa actcaagaaa attgcttttt aaatttttgt gaatttcagc
     1501 ttatttccga aggagttgct tctgcttccg ccgtttatac gtgtttagag cgtgagacaa
     1561 aagtgttctt tacttttgtc ccacgctctt ttttacagat aaacaatgtg ttagaagtag
     1621 tatcttcttt tacataaaaa gctgaccata ggataaatgg tcagcgtatt tagtgagtca
     1681 gctatttagt atgggttttc taaaggaacg actgtctctc ttttgatcaa gcgatgaggg
     1741 atgaccaaac gtacgccact gctcaattct tcatggatca attcgcgcag cttttgcata
     1801 gcgacacgtc ctaattcgga gatatcgata tcgatacttg taaggtaagg gtgtgttaat
     1861 gttgagaaga tcgagttgtt gaagctaatg acggagagtg tctctggaat gacatagcca
     1921 tacatttgcg ccaactgcat cgtgcgaagg gcgaagatat cgtcaatgac caccaatgct
     1981 gtggcttcgt tttctttcaa cgttttctca aaggacagat aatcctccgg atgctctagt
     2041 actacactgg ataacgcagg aagaccagcc atcatcattg ctttttgata accgaaatat
     2101 cgttcgaaat acaaactttc atgagtcgtg tttgtcacaa acaggatatt cttatgtcca
     2161 ttctggatca aaaattctgt tgcttgttta cctagaagct ggttatcatt atctacataa
     2221 acgatttcgt tctcatgatg ataaggctgt ccgatcaaag tgaaagggat gtggttttgg
     2281 tataaataat caatgacagt atcttgacta tctgaataca ctagaataaa accatctact
     2341 tgtttttgta aatgcatacg ctggacgttc tctaacagca attcaaaatt tttagcagta
     2401 gcgattgctg tagtcatatc ataattccgc gcttcttcgt tgattgcttc catgatctca
     2461 agataaaatg ggttaccgat ccgctctttt gaatcaagag ggggtaagat tacgccgatg
     2521 gcactggaaa tgcgttttcc tagattgcgg gcagtcatgt taggaacata acctagttca
     2581 tccataattt ttcggacttt cttctttgtt gtctcggaaa tactaggatg atcgttgatg
     2641 acacgagaga cggtcgaggt ggcaacaccg gcttttttcg ccacatcttt tacggtaata
     2701 gccatgataa aaacaccttt ctcttgttat tgtgcagaga accgataaat cgtctgactg
     2761 atccgttctt gaccaggatg gaggttgatc gatccaaatt tggggaaatg aacgatatca
     2821 gggatttctt gcggttcgat tgctaatccg tagtttgaat gcatctgttt tccattgaca
     2881 gagaaatcag cttcgaatcc agtcgtagaa aataatacca tacttttatt agaagttgct
     2941 atagtcattt ggcgaccaga atgcttctcg aataattgta aggaagtttt ggaaagccta
     3001 ggatgatgca aaagaaatac atcatctagt ccatctggat aacatgcaag gatctcttgc
     3061 agtgttgggg attttctaaa gtcgattggc agtttttctt tttttgtcca atttccagta
     3121 ggcagttttt cttgatctaa ttcaagcaca ccatctaaaa agaccgttaa ctgatgttca
     3181 tagatgtctc ttgttccatt tcctgaaagg ttgaagtagg catggttggt cggattgcag
     3241 atggtttcaa caaggctact agcagaggtt gtcatctcta attcgttttt tgttaaacgg
     3301 taagtgatcg tagcagtgat tggtcctgga taggtagaaa attcatcttt taagtaaaag
     3361 accacaccta ttgatgatgg agttttgaaa acttctactt cccaaaattg gaaagaccag
     3421 cctttcgtgc caccgtggat atggtgtgat ccgcagtttt tctccaatct atgctcaccc
     3481 caagaagcat cccggatcct tccggctaca ggtccgacag ttgctccaaa aaagcttttg
     3541 tctgttagaa catcttcgaa acgatcgtaa gacaataaaa cattttctga ttttccttct
     3601 ttgtctggtg cgaagatttg gtgcaggcgg gcaccgtaat tgagaagaac gacttctaag
     3661 tagtcgttct tcaatgtgat tttctttatt tcagtctctg gaactggtac gacggagatc
     3721 tctggattat ccatggtcat gccacttttc tttcaagtaa tcgatcgtaa gaaaagtcgt
     3781 atcagggaca agcgggatct cctctccaag gtcttcagca cgtcccacgc caattggttg
     3841 agcgccactt gagaggatgg cttggatacc ggtttgcgcg tcttcgatcc cgatacattc
     3901 ttctgctttt agtccgacag cttttgctgc aaggaggaag atatctggcg caggttttcc
     3961 gtttgttgct tctgccgggt cggcaatagc atcaaagtaa ggtgtaagac ccattttttc
     4021 aagtaggaat ggcccgtttt tacttgcaga agcaagagca atcttgattt tttcttcttt
     4081 taaagaatcc agtaaaggca cgaccccggg gaagacatct tttggttcga tcgtctggat
     4141 catctctaaa taataatcgt tttttctttt cgctaattct gcaaattctt cctttgaaaa
     4201 agagtgttct tttccgccat aagctaaaag caaagagagt gagtcttctc ggctgacgcc
     4261 ttttagctgt tcgttgaatg tgtggtcgat cgtaatgccg atttcctgac caagctttcg
     4321 ccaagcacga taatggaatt cagcagtatc ggtaatgaca ccgtctaaat caaacaatac
     4381 ccctttaaac atttgcttcc tcctttctta gcgttatttc tagacgatcc gctaaagaat
     4441 aagactgttc atagacgtaa atttctagag gagaaccttc taataactcg aaagtaactt
     4501 gttcatctgt gactgagatg aacaataaac gtccgcgata attgatatgg aaagaataag
     4561 ctttccaagt ttttggcaga aatggtgcga atctcaactg atcatgggca gttttcattt
     4621 gagcaaatcc ttggacaatc gacaaccagc taccagtcat ggaagtgatg tgcaagccgt
     4681 cttctgtgtc gttattgtaa ttatccaaat ccaagcgagc tgttcgctgg tacatttcta
     4741 cagctttgct ttccattcct aattctgctg ctaaaatcga atggatactt ggcgaaaggg
     4801 aagactcatg gacagtcatt ggttcataga aatcaaagtt tttttgcttt tcttcttttg
     4861 taaaagtatc attgaagaaa taaatcccct gtagtacatc tgcctgcttg atgaagcaag
     4921 atctaaggat tttgtcccaa gaccaatgct gattcagagg aagatcgctt gtgggaatat
     4981 cggatacagg catgagatct ttatctaaga atgtgtcatg ctggacaaag attcctaatt
     5041 cttcgtcaac tgggaagtac atgttctctg cgatatcttt ccatttggct agttcttctt
     5101 cgctgatagt gatagcagct tctttttgat aagttaaata attttctcgc gtgtaacgca
     5161 agacccaagc tgcgatcgta tttgtatacc aattgttatt gatattgttc tcatattcgt
     5221 ttgggccggt cacaccgtgg atcatgtatt tatttgctcg ttttgaaaaa tggacacggt
     5281 ctgcccagaa gcgtgcgatt tctgctaaaa cttccagtcc ttcatttttc aaatattccg
     5341 tatcgccggt gtaattcgta taattgtaga ttgcataggc aatcgcgcca tttcggtgaa
     5401 tctcctcgaa tgtgatttcc cactcgttat ggcattcgac accagtgaag gtgaccatag
     5461 gataaagcgc gccttttaat ccttgttgct gagcgttgtg gattgcttgc ggcagttgat
     5521 tatgccgata tttcagcaga ttcttagtta cttcaggttt ggctaaagca agataaagag
     5581 ggacagcata cgcttctgta tcccagtaag tggctccgcc gtatttttct cctgtgaatc
     5641 ctttggggcc gatattcaat cgttcatctt cgccgtagta agtagagaat agttggaaca
     5701 aattgaatcg aataccttgt tgtgcttctt cgtctccttc gatgaccaca tcggcaagct
     5761 tccatcgttc tgcccaagcc gctgtttgct cttcttttgc ttggatataa ttgtttgtct
     5821 ggttcgttaa ttctgagaca gctttttctg cttgctgcgc ttctggaata tctctactag
     5881 taagaacgat aatttctttt tccactgacc atgtctcacc ttgagataat tctgcagaca
     5941 agcaaccatg taatgctaaa gtatcttcgt gatagtcagg gatgactgat tttccattaa
     6001 cgaaatggcg cattgctgca gtaacagtaa attgttcaat cccaaagttg tttggaatag
     6061 ttttagtcgt caaatagccg atttcttcta tagttccacg atcaatctca ttccaaaaat
     6121 gttcatcata gttgctgtct tcgttttgaa catttccatc caattttgat acgagtttga
     6181 ctgttccgct accttcaagc atttctatag ttaaatagat ataagctgat tcttttttga
     6241 caatgctcag aaaacgttcg aaggaaatgc gtactttatt agtagaagta gtgattgtga
     6301 atgtacgaga taatacacca tttttcatat ctaattccca gtaaaagtct tctggttcca
     6361 aagaagccaa gtcgatcagc tgatcgttga tcgaaatatc tatcgcgata aagttcaagg
     6421 cgttgatcac ttttccaaag tattctggat aaccattttt ccaccagccg acacgtgtct
     6481 tatcgggata ccagacaccc gccaaataag taccttgatg gtggtcccct gagtaagttt
     6541 cttcaaagtt cccgcgcatc cccatataac cgtttccaat acttgttaaa gattcttgca
     6601 gtcgaagatt ttttttatct aattgtgtcg tgctgatttt ccatggatca atagcaaata
     6661 gtcgtttgat ctgtttcatt tttctcctcc taatgagctg ttttatacgt tcataataaa
     6721 cgcaaccgat tgcacaagtc aatagacaaa agaaaatatt tcatagaaaa aagcttaaaa
     6781 aaataaattt ttaggagaaa attatagaag ggaacacacg tattataggt tttatagaat
     6841 ggtatagaca gaaaaataaa aaataaagca tttacaaaaa agaaaacgca cgctataata
     6901 cgtttaacga aaccgattgc gtaaaggagc taaacggaat gaaaaagata tttagttttg
     6961 agttttggca aaaatttgga aaggctttaa tggttgtggt agcagttatg ccggcagctg
     7021 ggttgatgat cagtataggg aaatcaattc ccttgatcaa tcctgattgg acgccgcttg
     7081 tcacaacggg cggagtaatt gaaaatattg gttgggcaat tattggtaat cttcatattt
     7141 tatttgcttt agcaatagga ggaagttggg caaaagaaag agcaggcgga gcatttgcag
     7201 ctgggatctc atttatttta atcaatcgga tcactggggc tatctttggt gtcacaagtg
     7261 acatgcttgc gaatgaagat gcatttaccc acactctgtt cggtacgaag atcatggtta
     7321 aaggattttt tacaagtgta ttggaagcac cagctttgaa tatgggagta ttcgtaggga
     7381 tcatcgctgg atttgtcggg gcaatggctt ataataagta ctataattat agaaaattgc
     7441 cggatgcttt gtcatttttc aatggaaagc gctttgttcc gtttgttgtt atcttatggt
     7501 caacaatcgt ggcattgatt cttgctgtgg tgtggccaaa cgtacaggct ggaatcaata
     7561 attttggttt atggattgca gaatcacaag aaagcgcacc gattctagca ccgtttttat
     7621 ttggaacatt agaacgttta ctattgccat ttggattgca tcatatgctg acgattccta
     7681 tcaactatac acagcttggc ggatcgtatg agattttatc aggcgcacag gcggggacac
     7741 aagtatttgg gcaagatccg ttatggttgg cttgggccac tgatttagtg aatcttaaag
     7801 gtgcagggga tatgtcgcaa tatgagtttg tgttgcataa ctggacgccg gctcgtttca
     7861 aagtaggaca aatgattggt tcttcaggta tcttgatggg aatggctttt gcgatgtatc
     7921 gtaatgtcga tcctgataag aaagcaagat ataaatcaat gtatttttca gcagcgttgg
     7981 ctgtctttct aacaggtgta acagaaccat tggagttcat gtttatgttt gctgctgtgc
     8041 cattatatgt catttattca gtgatccaag gggctgcatt tgcgatggca gatatcttgc
     8101 cattgcgggt ccattcattt gggaatatcg aattgctgac tcggacaccg ttagctatca
     8161 aagcgggtct aggtggagat cttatcaact tcgtgttgtg tgtgatcgct tttggtgttg
     8221 ttacgtattt cttagcaaat ttcttgatca aaaaattcaa ctttgctaca ccgggacgta
     8281 acggaaatta cgacaatgac agtgaagaaa ccgttgtatc taacagcgga acaggaacag
     8341 cagatcagca agtggtccag atcattcatt tgctgggagg aaaagaaaat atcaaagatg
     8401 tcgatgcttg tatgactcgg ttacgcgtaa gtgtaaatga tcgagaaaaa gtagggactg
     8461 aagaagcctg gaaacgagca ggggcgatgg gattgatcgt caaagacaat ggagtccaag
     8521 cagtttatgg accaaaagca gatgtcttga aatcagatat cgaagatctg ctgcaatcag
     8581 gagcagagat tccagagcca gaaatggttt ctacaggaaa tggacaagca ggtagtaagc
     8641 aatacttagg tatagaacaa gaactagttt ccgcagcttc tggagaagtg attcctttat
     8701 ctgaagtaaa agatccggta ttctcacaaa aaatgatggg tgacggcttt gctgttattc
     8761 caacatcaag agaagttgtt gcgccgatcg ctggaaaagt gacaagtatt ttccctagca
     8821 aacatgcaat tgggctggaa acgaaagatg gtatcgaagt tttgatccat atgggaatcg
     8881 atacagtcca aatgaatcag ccagcttttg agattgctgt caaagaaggc caagaagtag
     8941 gaacaggaac gaaattagct gagatggatc ttgatgtgat ccaaaacgaa gaaaaagatc
     9001 caacgatcat gatcgtattt acaaacgata aagtagaaga agtagtgatc aagcaattag
     9061 gtacagtgac agctggtaaa gtgatcggct ccattaaaat gtaaggagag gttgtgaaaa
     9121 agctgtcctg catcaagtca taagaacagc caaacaaaaa tagctcctcc tatttttcgt
     9181 ttggttgggc ttattaccga gaagcagcag tttgaatact gtttagtgga gaggtcgtga
     9241 aaaagctgcc ctgcaccaag tgataaaaac agccaaacaa aaaatagctc ctggtccatt
     9301 gagcttatta ctgaggagtt agcaggagaa cactatttgc tggagaggtt gtgacaaaaa
     9361 ttttgtcaca acccttttgt ttcgaaatga agacaaggag aaaaccaatg aaattcatga
     9421 cactcaacac gcatagctgg cttgagccag aaccagagaa aaaattgcaa gaattagcag
     9481 ataagatcct gttagaagat tatgaagtga ttgctttaca agaaatcaac caattactgg
     9541 aatctgaaga agtagaacct gcgaagctaa tgaagttctg tccggtaaag aatcaagtac
     9601 ctatccgtaa agataatttt gcctaccgtt tagttcagct tttaaaggaa cacggaaaag
     9661 agtatttttg gagttgggaa atgagccaca tcggttatga taaatatgaa gaaggaaatg
     9721 cactgcttac gaaaaaaaca ttggaaagtc aggtccttac cgtctcccaa tttcaaaaga
     9781 aagaagatta tcagacacgc aagatcttga ttgggaaaac aaagatagat gatcaggaca
     9841 tttttgtctc gagctgccac ttttcttggt ggacagataa aaaaagtggc ttttattttg
     9901 agtggaaaaa cttggaaaac tactttttgg aaactcgcgt accacttttt ttcctaggtg
     9961 atttcaataa tccagtagac tctcaaggtt attatactgt aagagaaagt tgccttcttc
    10021 ttcaagattc gtatgttgtc gcgaatgaaa aaggaaaagc agctactgtg gaaaagaaaa
    10081 tagatggttg ggaacaaaat acagaaaaat tgcgtataga ttttattttc gtccctgaag
    10141 gaatgcaggt taaaaaatat caacggattt ttgatgggat cgatagtcca atcatcagtg
    10201 atcactacgg ggtggaaatc gaattagatg tgaacgaata aaaaatataa catataacat
    10261 aaaaaaactg attaaaataa aactctccaa acgattatac cgtttggaga gttttttgat
    10321 cggatgtatt tatccctttt gcctaagcac gtaaaagctt gtatctatga taaataaata
    10381 aacatagcaa ggtaaaccag ccaatcaaag taagagaaag aatgaaatag aaatagattg
    10441 gcgtttgata atgaagcgtg agctcgttga ttcctttttg ctgcttcacg attggggtcc
    10501 cgatatctgt tacttcataa tcagtcaatg tttttccatt ttgctgtagc acagtccgat
    10561 cgtagacgat aacagggata tgaacagttt tgtccccagt gtttttccac ttgattgtca
    10621 aaagaccgcc tggatgtgtt tttataaaag ttgtttcgtt ttctagtatt tcttcgttat
    10681 accggtcgta tttattgtct tttgtctcct tatagattgg cagatagtca ggtgtggttt
    10741 tttggaaaac gaacaagctg gtagacatat ctttgctgaa gaatgttttc cttgcttctt
    10801 ccggcgtatc gaatagataa gtatgtcgac tgacaaatgt ttcattttcc cacttttcca
    10861 gatgctggta taagttctgg atcgtctgtg ctgaaaaaat aagaatgcca aatatactta
    10921 aaagacggaa ccacttttgt tcagaccact ggttcagtga aagggcaagg taaagtagaa
    10981 gcaatagcgt aaatggcacg aaaaatcgaa atggaaactg gatcaaacta ataaaaggca
    11041 catttttttc gatcaatgtc gtccatggaa tcaagctagt agaaagaaaa aagaaaacaa
    11101 gacttgtgta caacacgatt ttttggaata aggtgttctt ttgttgaaag aaaataaaaa
    11161 gcaaaaaacc tgataatatc aatataggaa aggccttagg gtaatggagc caataactgc
    11221 catccagatc tatcgccttt tgactcattt ctctgttaac aaagggagga agaatcttat
    11281 tcccactgta aacaatagct aatcctgccc aaatatttgc tgtcagaaca aaataaagca
    11341 agaccgactt aatcagttga agcagtgctt ttcctttttc agtctggtta aaaaagtaat
    11401 aaagataaaa agggatatac atcaaagcta acatgatcga agacagtaaa tggatctgtg
    11461 tcataagtcc cactgagaca gcgactttga taacagggaa ctttttatga aggacgaaat
    11521 cagcaatagg gatcatacat aaaggataaa aacaagtccc ccagctagtg aatccttggt
    11581 tgatagacca gtattgaata gaatacgtac tcatgaataa aacggctact ggtacactta
    11641 cccaatttcg aacattgaca tatctcagta aaatcagcat agaaaatcct gaaataagat
    11701 aaagaatgaa atttgaaaga atctgataat gaaaccagct acctgcaagc aacacgatca
    11761 atccttgtaa ataagcgaaa aatgggccgt aaacggcatt cacgatcctg ccactttgct
    11821 ggaatccata aagggaagga aaataatgga aatccccata tttaatttgt tgcgcggcat
    11881 catagaaccg attgtaatgg aatggggcat cagcacccaa aattagttta tgtgtgaata
    11941 aatgaggcat gagaaatgcc aaagcaatga aaaaaattgc taaaaatggg ccatattttt
    12001 tcaattgtcg aactcctttg caaactttag ataaattcat catatatcct atgcgagagg
    12061 taggcaagaa attagaaaat tcatgaaaat atttatgaat tttacaagaa aaacataagt
    12121 gaaagttcta aggagagagg atgcgacata agttttgttg catctccttg ttttgaaaca
    12181 gaatttttct taatactcga aacaaagtag ctttttcatg acctctcggt aaacggtgtt
    12241 caatcagctg cttctcggta ataagctcac tagcctagaa aatatgagaa gctattttct
    12301 gactagcgct cttattactc aaagcataac gcagttttca caacctcttc cttaaacggt
    12361 gttcaaaagc ttgcatctgc ggtaataagc ccaacccaac aaaaaatatg agtagctatt
    12421 tttttgggct gtacttatta cttgatgcag gacagctttt tcacaacctc atcggctttc
    12481 attttctatt tttttcctga acccatataa taaaagaaag aacaaaaaaa cttgcaaggg
    12541 aggacacatg tgaagcagag taagcaaaaa cagtctcgtc gacttttgtt tcgtttgttt
    12601 ttattaatat gtatcgtcgt agggttactg atcgctttat atccatttta tgtggattct
    12661 ttaaatagtt ttattgatca aaaacgtatc gaacaagtac aaaaacggac cgcagctgaa
    12721 attgaagtgc agcgaaagaa aatggaggag aaaaaccaac gattaactga ccaaggattc
    12781 aatcctggta ctgatccttt caatgaaaag aacagaaatg aaagtacaac atccagccaa
    12841 ttagaggaat ggctgatcgg aagtatcaat atacctaaga tccaattaaa tatttcactt
    12901 tatgatcggc tcaatggtag gatccttgaa aatggagcag gcgtcttgca aggaacctct
    12961 ttcccgcttg gtggaaattc gacgcattca gtgatctctg ctcacagcgg tttacctaat
    13021 cgacggttat ttaccgaatt agatcgttta gaaaacggag acacgtttat tttgactgtt
    13081 ttgggggaga aattggctta tcaagttaaa aatatacaag tggtactgcc agatgacacg
    13141 tctgttctaa cgatttcaga aggagaagac ttagttacct tgcttacatg tacgccgtat
    13201 atgatcaaca cgcatcgttt gctagtgaca ggacatcgga ttccttataa cgaatcggta
    13261 aaaaaagaag aaggaaaagg aaatcaggaa agaaccatgc ggcaatggtt tattcttgca
    13321 ggaacgataa tagctgtagt catactgtta gtgtttatcg gacgactaat ctatcagtat
    13381 agattaagta aaaaagtgct tgatttttca tttattatta gtgattcgtc agggaaaccg
    13441 gtaaatggta gttcattcat tttgaaacat aagaaaaaaa cacttactag aaatggtgtg
    13501 ccgttttcag tacaaagcga ccatcatggg aaagtcaagc tggatcaatt gcctggtgga
    13561 acttatcgaa tcgtttcagc tgatgatcct aaagttgctg tttcttttgg tattagaaaa
    13621 gtaaaacaag agaagatata cttttttgag gggcgaaaac ttgtaaaaga gcttcaaaaa
    13681 aatggttttt attttaaaat caatgaataa gtaaggagag aaaaaatgat ccgcacaaca
    13741 acaaaattgt ctgccaaaga actggaatct gttctatcga tttggctgca tgctaacttg
    13801 gattctcatg aattcattcc atcaacttat tgggagaatc aagtagaatc tgttaggaaa
    13861 gctttaccag atgcagtgat ttacttagca gaaatagatc aagaaattat tggttttgct
    13921 ggggtgattg atcagtttat cgcaggaatc tttgtgaaaa gggaataccg gcaacaaaga
    13981 atcggtacgc aactaatgaa aaaaataaaa gaaaccaata aaacactgac tttgacagtc
    14041 tatcaaaaaa atcaatcagc tattcatttc tatctttcac aaggttttac aattcaaaca
    14101 cagcaaaaag atgaagcagt aaatgaggag gaacttgtga tgaaatggaa tcaaaacgct
    14161 gatttttgat gaacagaata acgaaataat gctcccagcc taattttttg ctgggagcat
    14221 tatttcattt tgaagtcact aatttaactg tgtgaatatg cagttgttac aatctttgat
    14281 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
    14341 ctatttttcc atttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttga
    14401 taaacggtgt tcaaacggct gcttctcggt aataagccca ctagcctaga aaatatgaaa
    14461 agctattttc taactagcgt tcttattact caaagcataa cgctgatttc acaacctctt
    14521 gataaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaacatgag
    14581 gaactatttt tccgtttctt tctctaatac tcaggtcaga acgctgattt cacaacctct
    14641 ctttcacaac cttattttac tcgtttataa ttttcatcga agtattttcc gctacggata
    14701 tcatctggca ttccatcgta tggttcttcg ctaataacat cttcattgtc tatacctgca
    14761 tcacccatat aagtgatcgt agcaaattca ggaacaagta attttggata agtttcatat
    14821 ttttcacgag ctgcttccat ttctgcgata tgttcgtttt ggaaagtaac agtgatttct
    14881 ggatgttctt caacccattt agggaaaaag atcattccgt gaagtttctt ttcatttggc
    14941 ataaagtcca atgccacgtc tgtccctgtt ggctctgtcc aagtgatttt gaatacgccg
    15001 tccgtcaatt taacgatatc agcttcttgg tcttttaccc aacggccttt gaccattccg
    15061 ccatggatac ggtaatcaac agtatggtca tttttggcat accattcata ttcccaacca
    15121 ttgtcatacg tatagataaa gtgagttcca ataaaatctt ccaattcttt aaattctttc
    15181 atgtttaagc atctccttat aaattaaaac atgtaataaa ctacatgttt tcgattactc
    15241 atttatgata gaatggattt aaagatttgt caaggaggga aaactgatgg caagaaaaaa
    15301 tacacttcga tatattttgt tagggttatt aagtaagaag aagatgacag gatatgaatt
    15361 gaatcaatcg ttcaaaaatg aaataggaga attttggcaa gcaaaacata gtcagattta
    15421 tcctgaactt gcaaaaatgg aagagcaagg aatcattcag catcaagtag agatcacagg
    15481 tgaaaaattg gaaaagaaag tttatgaatt aacagaagaa gggaaggaac aattggcaga
    15541 gtggattcat acacccacca atgaactacc ggttaatcga gacgaatttg ttctgaaatt
    15601 gtatttcgtc aaagacgtga atgatccggc attagtagaa attattcagc agcagcgtga
    15661 ccttcatgaa gaaaaacgac ttcatctatt agagcgaaaa aaaacgatct ttcctactga
    15721 aaaagaaaaa catgaaaatt acggacacta cctgatttta gcgcatgcga tcaatcggga
    15781 aacagaatat gcaaattggc taacagatgt attagaagaa agtaaccgaa aaaaataaaa
    15841 aaatatccga acagtgatgg agaattcctt tctatatgaa agaaacgcag atcaccgttc
    15901 ggatattctt atgttcaaac aggccttttt tcaagtattt atgtagcatc tacttattaa
    15961 aaagcttggt acttaggaga ttagtggcgt ttagagttct tttaacaact gttcgtagtc
    16021 agctttttca tcgacaggaa ggagcttgta agttaattcg tctgtctcgt tgtcatgaat
    16081 gatgtacatc ttagcattct cttctttgaa gttaccgatt tgtaaaatcg tatagcgttc
    16141 gtcgatcaat tcgatatctt tgattgcttt tgaaaaaact ttacggacat ggtgcatttt
    16201 cttttggcgg atttctgcct tttctgcagc ttttgacaaa gtaaatccgt catctaaata
    16261 ttttttgatc agttctactt ttatgactgt aggtaaacga tattttctag gacaattcgc
    16321 atcagcttcg atcgaacgaa tgaatccctt ttgttcccaa taccttaatt gacgtggaga
    16381 cacacctacg atatcactta attcactgat gccaacaacc aattgatcat tttccaacaa
    16441 acgtttaagg gaagccccaa ccatttttgt cactcctttt gtcctcttgc ttcccttaat
    16501 tatatccgta ttaacttatg tgtcaagtag gttgacaaat agcgaattta ggactaaagt
    16561 attctagtag tttcagaaaa aattcagaaa gaaggagaaa tatgagtaac aaccaacccg
    16621 tagatattta cggaaaacca tataatcgga cacttttagt tgttgtctta ttaattggta
    16681 cattttgtac cgttttgaat cagaccttat tgacgacagc ttttcctaca ttgatgaaag
    16741 ctttcgatat ttcagcgtct gatgtgcaat ggttaacgac tggtttcttg ctagtcaacg
    16801 ggatcatgat cccaataaca gcatggctga tcaataaatt cagttcaaga aatttatatc
    16861 tttctgccat gacgatcttt ttgatcggaa cgattacttg ctttacagca cctaatttta
    16921 gtacattact aatcggtcgt ttgatccaag cttgcggtgt gggagtatcg atgccattat
    16981 tgcaaaatat catgctatct attttcccgc cagaaaaaag aggatcagca atggggatgt
    17041 cagggatcgt tattggactt gctcctgctt taggccctac attatctggt tatatcattg
    17101 ataactacca ttggcgtgac ttgtttggta tggtactgcc aatcgttgtt cttgtcttgg
    17161 ttttagcatt cttcctaatg aaaagtgtta ttccattgtc aaatcctagc attgatattt
    17221 tatccgctat tttatcaacg attggattcg gtagtttact ttacggattt tctagtgtag
    17281 gagatgacgg ctggggaagc ttgaaagtaa ttggtttctt agttgttggt gtgattttca
    17341 tcgccttgtt ctcatggcgt caattacatt tggaacatcc attcttggaa cttcgtgtat
    17401 tcaaatcatc tacattcacg attgcagctg ttttagctgg tgtaacaaat atggcgatgg
    17461 taggggcaga aatggtcgta ccattgtata ttcaaaatat tcgtggagaa tcagcattcc
    17521 attctggctt gatgttgttg ccgggtgcat tgatcatggg ggctatgatg cctgtgacgg
    17581 gtgcgatttt cgataaatac ggcgcgaaac gtttagccat cacagggatg ttcattttga
    17641 cagcggcaac tgcaccattt gctttcttga caaaaacgac tccagtttta tatatcgtga
    17701 tcctttatgc gatccgtatg tttgggatct ccatggtcat gatgccggta acgacttctg
    17761 gtatgaatgc attgccaaat aacttgatca gtcatgggac agcggtaaat aatacattcc
    17821 gtcaaatcgc aagctcgatt ggtacagcta ttttgatcag tgtgttgacg aatgtgacaa
    17881 aagcaaatat cccaagcaaa tctgtattgg ataatacgcc attagcttat aaagatcagg
    17941 caatcaatgc cacactgtca ggttaccatg ccgcgtttat cgtagcgatc atctttggga
    18001 ttcttggctt tattattgcc tttttattga agaaaaaaga acctgttcta atgaaagcgg
    18061 gggataatgc atgattattg gagttttgat tgtcagcttg cttgcttttg ctttaacgaa
    18121 cgtgttcgct aaaaaaacat ggcaaacctt tttatctttg attttcggac taatcttcgt
    18181 cgcttcacta agtttgatcg tcgctaattt gtccaatcac tttggaatgg aaaaagtaac
    18241 ggaaacaaaa acagaaaaaa ttgtttccag tgctgattcc caaggagcag atatgctttt
    18301 gtataaagct ttagggaatg gaaaagaaaa agtgtatctt taccgaacaa atgaaaaaca
    18361 agaaaaacca aaagcaactg gtacggataa cgaaacaaat aaagtagaaa agacagacgg
    18421 cgatgctgaa aaagtgacga aaactactta ttgggaatac aaaaacgaca tgtataaatt
    18481 ctggttcaat attgctgata acaatcacga atatgacagc cgagtgaata cattcaaaat
    18541 cccagagaca tgggtagaat taagtacgga ccaagcagcc aagttagctg aattagtgaa
    18601 gaaacagcaa agtacgatgg aaagtgaagc aaaagcttat gttcaagacg gtatggtaaa
    18661 agcaatgact gaaaatccaa aaatgagcaa agcagagcaa gaacaacgta cgaaagcctt
    18721 agctgctgaa ttccaacaac aagcattcgc aaaattagtg aaagaagcaa aaggcgaata
    18781 atattttgaa caccatgtag gaggttgtga aaaagctgct ctgcatcaag cagtaagaac
    18841 aatcagataa aaatagcact ccatattttt catctgattg ggcttagtgc cgtagatgca
    18901 agcttttgaa caccgtttaa ggaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    18961 ggagcaattt caaaaaacag cttctcatgt ttgttgacta ttgtgacttg tgaccgagga
    19021 gttggcagga gaacaccgtt tattcgaaaa aaggaactgc gacaagactt ttgtcacagt
    19081 tccttctttt ttttgctatg ctatactaga tgtaaaaaat aggagaaata aaatgaggga
    19141 aatttattct tttgaatcaa aaatctatgc ttccgaagtc ggcaagggcg gggcatatat
    19201 tatttttccg tatgatataa gaaaagaatt cggtgctgga agagttaaag tccaagcaac
    19261 ttttgacaat gtgccgtatg aaggcagtat cgtgaatatg ggcgtgaaaa atccagatgg
    19321 ttcagtctgc tacatccttg gactacgaaa agatattcgt aaaaacatcg gaaaagaaat
    19381 cggtgatatt gtagcagtta cagtgaagca aaaataaaag aggttgtgac aaaagacgtg
    19441 tcacaacctc ttgataaacg gtgttcaaaa gccagtttct cggtagtaag tcaaatttat
    19501 gcaaaaatgt ggaaacccat gtttacaaaa tttttcttac tactcgaaac gaagcggctt
    19561 tatcacaacc tcttgataaa cggcgttcaa tcagctgtcc ctcggtatta gctcaaaaac
    19621 ggcaaaatat gagaagctat ttttccattt ttttctctca tactcaggtc ataacgctga
    19681 tttcacaacc tcttctttca taatggtttt tcaccatctt tataataaac tggttctttg
    19741 atatggaaat ctattttttc caaccaatct aaaatcttgt aacctaactc ttctgacatc
    19801 ggaaaagagg cgttgatttg tgcattggag atatggtcaa catggatacc ggaagtgatc
    19861 gtacaacttc ccggaacggc agttttgatg atcacagcta ttttttcggc taagacatcg
    19921 tctttatgga accgaccatc gtggctaggg aaacgtacag tttgcatctc tgtatcacga
    19981 gaaaatgtag tgactgttcc gatatgtggt gtatctccgc ctgttaaagt gatcaataag
    20041 tctttaccga ttactttaac agcagcttgg atcgtgaaac ctagtttggt tacttcaact
    20101 atcgtttctt tttccatatt aatcaccctg ccaacgtttt gaatgctcat ttacaatttc
    20161 aaattgatcc aaaagtttca ttgtatggtg attaacaaga tcttggatcg tttgaggctg
    20221 attataaaaa gcaggaattg gtggtatgat ttgcacacct agtcgtgcta atttagtcaa
    20281 gttttccaag tgaatggcat ttaatggcgt ttcacgagga acaatgacta actttcgttg
    20341 ctccttcatt gttacatcag cagcacgtgc aatcaggttg tcgcctaaac caacagagat
    20401 acctgcgact gttttcatac ttgccggtac gatgatcatt ccatcggtca aaaatgatcc
    20461 gctggcgaca gcagcaccta aatctttgtt tgaataatgg taatcaaaca ggctttcgaa
    20521 ttcagttagt gataagtcgg attccagctt taagttctgt tttgcccatg gactgatgat
    20581 tccgtgtgtt tctatcatcg gatattcttt tagtttcttc actaggttga tggcataaat
    20641 ggtgcctgaa gcgccactta caccaatcac gatttttttc ttccgttgtg ccaattgttc
    20701 cattttccgc actccttttt atgatgtcaa aacagttgca gatcattcat tatttcaaat
    20761 atttttccca atcttctact tcttggaatt ttgcacgtac aaaatgttct ttcatatcga
    20821 aaggaactgt accgtcaaag atagtttttg cacttatccc acggaaacga atagagccag
    20881 gattatattc tggtaattct gatgggtcca atggatggtt cctcattcct gggatcacga
    20941 tgatatcttt atctccttgg aaacgagtgt tgaaagtcca aactacatcg ttcatgtcga
    21001 aaatatcaac atcttcatcg actaaaatga ctgtcttcaa ttctttgaag gctgataaag
    21061 caagaatagc tgcttgacgc tgaatacctt catccgcttc gttttcttta cggatttgca
    21121 tgatggtcat caatttacca ccacctgcag gaggattgta gacatttact acttttcctg
    21181 gaatagcttt atcgaccaag gcaagaatac ttgcttctgt aggaataccg gccatagata
    21241 cgtgttcttc actgggacca atcgttgttt gcatgattgg attgtctttt cggtgagtaa
    21301 ctgctttgac tttcactact tgtacagctg gattagcatc cccagtatag ccaggaaatt
    21361 ctggcattgc ttttcctgag ttcgtgttga tatcttcttg cattgtttca tttggcatga
    21421 tttctgcttc aatgatgaat tcagcacgag caatacccac ttcatcgact gctataccat
    21481 caacgatttg aataggttcg ttacgcaaag ctcctgcaca ccatagttca ttgtagccaa
    21541 gtggagtggt aggtggttcg aaagtcgtgc caatggcaat tgctggatct aaaccaatat
    21601 tgatagtgat aggcattggt ttattcaagg cttcgaattg tttttggaag tgaccaatgt
    21661 ggcgtccgcc aggcatgatg tacataccga tcgtatcttt atcttctaga accatacgat
    21721 ggatcgttac atcgctcatt gttttttctg gatctgatcc taagactaca cccattgtga
    21781 tgtatggacc agcatcttca ggagtatttg taggtgcagg caaaatgttg cgaatatcga
    21841 agtctgcatc ggtagctaga tgaacgactt cttgtgctgg agcgtctgct ttgtctactt
    21901 tcacaggttt gattggattt tctacagcgt ctttcaagaa acgaccaagt gttcgataat
    21961 catggtgcaa aatttcacct acacgtttac gactagccat tgttccgatg ttcacacgtg
    22021 tgccggggaa acctttgata ttgttgaaag tcatagctgg tccttcttga gtcggacgtt
    22081 ttactgtacc gccagcaccg atataacggt aaacaccaga taattctgct tcaggatcga
    22141 tttcaatatc tgtttcgtga tattgacctg gaagtgtttt tagttcttcg atgacttttc
    22201 ttaaatcata cggttgatct gtcatgagag attcctcctt agttgtcttt acctatctat
    22261 agtaaaaggt gaaaaagagg aaaacaattc aacttgttcg gtaaagtatt ctacaatgga
    22321 atggatgcgt ttttttgata atagaataaa ctatgatata cttatcacta aaagaaagcg
    22381 catacttagt gttggaggaa ggaaagtgaa aaaaatggag gagtcatttc ctaaagcagt
    22441 aaaagtggaa aatatagcga atattttgaa agttacattt gaaaacggag aagtaaaata
    22501 cgtgaaatct cattggacag aagagataac cgatgccttg caatttggaa aaaaaggaag
    22561 aggaaaaaga aaaaatcttc tcgcactttc tcgtaatatg tggattggga ctgaagtaac
    22621 gattgaagca gatggaacag tattcataaa tggaaaagac aggtatacgc cagaagagtt
    22681 gtggtacaaa ggaaagaaaa gtataccaga attataaaaa ataattaaaa aaaacagcgc
    22741 gaaacaagga acatattact tcctcgtttc ttgctgtttt ctactatttc agcgctctta
    22801 tgactcaaat ctaaatgtgg ttgtcacaac cttattctgc acgcaaagcg atagcggcgt
    22861 cttttttcgc tgccattcgt gcaggaagat gaccgccaag catcgtcaat atagtagaga
    22921 cgatcactag aataatcgca tggacaggat ttagttgagc aacatttttt agatccgtca
    22981 tgttgtataa gatgctattg atagggaacg tagctagcca tgcaataaac acacctaata
    23041 tcccagaaga aatacctaaa atacacgttt ctgcgtcaaa tacgcgagtg atgtcttttt
    23101 tacgtgcacc taaagctttt agtacaccga tttcttttgt ccgttcaatc acagatgtat
    23161 aagtaatgat actgatcatg atcatacttg ttactaaaga gatccctgca aaagcaataa
    23221 ggacataagt gatagcatcc atcaatccgc cagtcagttc agtcatcgtt ccagctaaat
    23281 cagtataaat gattttatct tcatctgatt tgcctttatt gtaatcatct agataatcca
    23341 agatcttttc tttgtcttca aaattgttcg gataaatcat gatacttgaa ggcaagctgt
    23401 ctccacctaa ataactaagc aatgtttgtt tggcattttc gtcaactttt tcagtagtta
    23461 agacatttac accactgtct ctttgagctt tgacgatttc tgattctttg ttttcgttga
    23521 cgatttgagt cgttaattga tcgctataag cgattcctgg agataagaga ttcattgtag
    23581 aagaagattt cacgcgtagg atccccgaga tcttcaattc atcagaagca ttctgataca
    23641 tcgcatcata atctgtatta gggataaaat tcccagttgg aagttttgta tagtaagtat
    23701 tgttgtttac taatttgaat gttgtaccaa cgatatcatc aaaatcaagt ttttcgtctt
    23761 ctttcacatc aaatccaaga ttcttcaatg cgttgatatt agtattatta ttgccgtcta
    23821 cgatcagcac gacatctgtt gctgaggctg gataacttcc ggcaagtaaa gaataattgt
    23881 cttttaaaaa gttttctttg ctagtgtcaa gctgtgtagg gaaacttgac actcccacac
    23941 ctgtcattgc tgacatcgta ctagataaag aaagagattc cgtatcagga ttttgattcg
    24001 agaagctgac aggctgtact ttgccattta catcacgtaa aagattgatt cctgtagtac
    24061 gggtaaatcc gatattatta cttaagttag gatcgatgtc tgttacgtaa tcgacgtatg
    24121 tttgatcaat cttattcgtg tgctgtgctt tatcttcttc gctgactttt gcagtaatcg
    24181 ttttgctgtc tggataatcc gctttgcttg cacccaatcc ggcatcgtca cgagtctggt
    24241 tagtcgttac cttagaaata gtaatcggaa atttcgccat cgtttcagct tgcgtgttat
    24301 cgatttgttt ttggaatcca gaagaaaggg acagaacgat cgcgattccg atgatcccaa
    24361 tactggaagc aaaggaggtc aagaaagtac gtcctttttt tgttcggata ttgttgaagg
    24421 aaagctttaa cgctgtccaa aaactcatct ttgttctgcg taagttaaat tgatcatctt
    24481 ttggtcgttc gatgtgagga tgggaatctg ttaagatctt accatcagaa aattcaatga
    24541 ttcgatctgc gtattgatgg gccagttcag gattatgtgt gaccatgatc actaattttt
    24601 ctttcgataa ctcttggatc aattccatga tctggatact tgtttctgta tctaacgctc
    24661 cggttggttc atcgcacaaa aggatatctg ggtcattggc tagtgctcga gcgatggcca
    24721 ctcgctgcat ttgtccacca gataactgat ttggcttttt atgcatatga tttgtcaagc
    24781 cgactcggtg caatgcatct tctgcccgtt ttcgtttttc gtctttagaa acaccactta
    24841 atgtcatccc aagttcaacg ttttcaataa tgcctagatg accaatcaag ttgtaacttt
    24901 gaaaaatgaa accgatagag ttgtttcggt aggcatccca gtcactgtcc ttaaagtctt
    24961 tagtagactt tccgttgatt accatatcac cggaatcata attatctagt ccaccaatca
    25021 cattcagcat agtcgtttta ccagaaccac taggacctaa aatcgctacg aattcttttt
    25081 gtctgaaagc gacagacaca ccatccaatg ctttggtggt ggtttcgccg actttataat
    25141 atttttttat atcttttaat tgaagcataa tcacccttct cccgtattga aatattcggt
    25201 atactaataa gtatatcaga acaatgatcg tagtatacga aataaatgtt aactgaagat
    25261 gaactacaag aaaaaatcta gaaatttttc aaaaaacaga gggagatcaa tgaatcgtat
    25321 tttagtagta gaagatgatc cgcaattaca gttgctttac cgttctgtgt tggaacgagc
    25381 gggcttttca gtcatcaccg cattgaatgg agaggatgcg ctgaaaaaac tagaagcgac
    25441 acgtatagaa ttaatcatta cagatatcat gatgccgaaa atggatggct atgacttgct
    25501 agactcatta cgtagcacca gatttgacac acctgttcta atgattacgg caaaggccga
    25561 ttttgaggat aagaaacgcg ggttcaaatt aggagcagat gattatatga caaaacccat
    25621 cgatgtcaat gagatgatcc tacgtgtgga agcattgctt cgacgttcaa agatcaatta
    25681 cagcagtagc ttgataatcg gacaaacaaa attagagcta gatgcttata cagtgttaca
    25741 agatcagcat tcgttgattc tgcctcaaaa agaatttctg cttctgtata aattactgtc
    25801 ttatcccaac aagatcttca ctcgccaaca gttgatggat gatatttggg gattagatac
    25861 gaatacagaa gaacgaacga tcgatgtaca tatcaaacgt ttaaggaccc gttttgaaaa
    25921 gacggaagat tttcaaatca tcacagtacg gggactaggc tataaggcgg ttatttaaag
    25981 atgaaaaaaa agctattttc acaattatgg atctattttg ctagtattgt cttcatttcg
    26041 atcttagtga cactgctgtg ttttctcggt tttattttct tattgtctcg acattctatc
    26101 tcgcctgctg aatcggctgg aaaaccaacg ttatacccgt tattcgtctt tgcggggttc
    26161 agtatggtcg tgggtacagg aatatcgatt tttgttgggc ggcgtatcct gcatccaatc
    26221 agtgcgctag ggacaaatat gagtctggta gcaacgggag atttttcgat tcgaatggat
    26281 gaacagcaga aagtagcaga agtacagcaa ctgtataaag atttcaacgt tatggtccaa
    26341 gaactcaata gtatcgagac actgaggaat gactttgtgt ccagtgtatc ccatgaattc
    26401 aaaacaccac tagcaacaat ccaaggatat gttcaattgt tacaagcccc aaatctctct
    26461 gacgaagaac gccaaatatt tttatatcgg attatcgaaa gtatcacgca gctttcacaa
    26521 ttaacagaga acatcttaaa attgaacaaa ctggaaaacc agcgtatcca actggaaaag
    26581 aaagagtatc gattagatga acaaatcaga gaagtaatcg ttttttttca gcctaaatgg
    26641 gagaaagaac agctagaatt ggatatcgaa cttgctgcag taaattacac aggcaatgaa
    26701 gagtttttgt accaagtgtg gctgaatatc atggataacg ccataaagta caatcaaatc
    26761 aatggacaga ttcatatcaa actatttgag acagccacag agattgtact ggaagtgacc
    26821 gattcaggtg taggaatgaa tgaagagacc agagatcgaa tgtttgaaaa attttatcaa
    26881 ggagatacaa gtcgtcaaat ttctgggaat gggctaggac tttcactggt taaaaaaatt
    26941 ctagaactgc atgatggacg tattgattat agcagtatcg aaggagtagg aacgacagca
    27001 atgatacggt taagtaaaca ataggaaaaa gaataaaacc cctaaaaatt ctattgaagg
    27061 tttttagggg tttttagtcg tttttttgtg attagggaaa tgttcttacg gttcttttgg
    27121 cataaaaatc gctgctttct tattgaacta cgagaaatgt tagaggtgtc attgaaacaa
    27181 ggacatgatg caagaaagag agcgaaagtc atgcgtcgct ctcttttttt gggtggatag
    27241 atggacagaa aatgtagggc ttagttttct tcttgattta aatactccca aaatgtatct
    27301 gaagccaaag aatgctcacc gactttacga gtaaatgcaa gataactctc tgttgttgga
    27361 gaaataggga gaatcacagt attttctttt aatagattct tgactgtttt ttccataaca
    27421 atagcaatcc ccatgtcatt tgcaatcata ttgatgatca agtcgattct tgctccttta
    27481 taagagattt ttggttcaaa tccagcttcg cgacacagat ctagtacagg ttgatacaag
    27541 ttcgtagtgg ggccaaggag caaaaagttc tcctttttca gttcgcttag atccaatttc
    27601 tttttactag ctaaaggatg atttttaggt agaactgcta caaaatcgtc tgtttcagta
    27661 ggcaaccact caatcgaact tggtggggcc tggaaacttc tagcaaagtg gatcgtgtca
    27721 ctatgttcat tggcgtcatt gtaagttaat tcgacacttt ccacttcttt caactggact
    27781 gtaaattcag gatggcgttt caaaaactcg gtgattttgg aaaaaccttt ataattgatc
    27841 attgtcggta tcgttaagat
//
LOCUS       DAPBCS010000036        27787 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPBCS010000036 DAPBCS010000000
VERSION     DAPBCS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.036"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(18..293)
                     /locus_tag="RD521_001820"
     CDS             complement(18..293)
                     /locus_tag="RD521_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771533.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFRKQMKKLIEKEFPRSHKLYVNQQF"
     gene            587..1402
                     /gene="thiD"
                     /locus_tag="RD521_001821"
     CDS             587..1402
                     /gene="thiD"
                     /locus_tag="RD521_001821"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDT7771534.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFKLIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1607..3058)
                     /gene="cls"
                     /locus_tag="RD521_001822"
     CDS             complement(1607..3058)
                     /gene="cls"
                     /locus_tag="RD521_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDT7771535.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3400..6066
                     /locus_tag="RD521_001823"
     CDS             3400..6066
                     /locus_tag="RD521_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7771536.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6430..7182
                     /locus_tag="RD521_001824"
     CDS             6430..7182
                     /locus_tag="RD521_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7771537.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7204..8358)
                     /locus_tag="RD521_001825"
     CDS             complement(7204..8358)
                     /locus_tag="RD521_001825"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDT7771538.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8543..10984
                     /locus_tag="RD521_001826"
     CDS             8543..10984
                     /locus_tag="RD521_001826"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDT7771539.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11314..12018)
                     /locus_tag="RD521_001827"
     CDS             complement(11314..12018)
                     /locus_tag="RD521_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771540.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12205..12450)
                     /locus_tag="RD521_001828"
     CDS             complement(12205..12450)
                     /locus_tag="RD521_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771541.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12467..13318)
                     /locus_tag="RD521_001829"
     CDS             complement(12467..13318)
                     /locus_tag="RD521_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDT7771542.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13483..14487
                     /locus_tag="RD521_001830"
     CDS             13483..14487
                     /locus_tag="RD521_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDT7771543.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14728..15084)
                     /locus_tag="RD521_001831"
     CDS             complement(14728..15084)
                     /locus_tag="RD521_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7771544.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15452..16639
                     /gene="tuf"
                     /locus_tag="RD521_001832"
     CDS             15452..16639
                     /gene="tuf"
                     /locus_tag="RD521_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDT7771545.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16827..19418)
                     /gene="mprF"
                     /locus_tag="RD521_001833"
     CDS             complement(16827..19418)
                     /gene="mprF"
                     /locus_tag="RD521_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDT7771546.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19444..20205)
                     /locus_tag="RD521_001834"
     CDS             complement(19444..20205)
                     /locus_tag="RD521_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7771547.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20372..21004
                     /locus_tag="RD521_001835"
     CDS             20372..21004
                     /locus_tag="RD521_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDT7771548.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21063..21923
                     /locus_tag="RD521_001836"
     CDS             21063..21923
                     /locus_tag="RD521_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDT7771549.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22108..24423
                     /locus_tag="RD521_001837"
     CDS             22108..24423
                     /locus_tag="RD521_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7771550.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24605..26446
                     /locus_tag="RD521_001838"
     CDS             24605..26446
                     /locus_tag="RD521_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDT7771551.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26470..27579
                     /gene="rpoD"
                     /locus_tag="RD521_001839"
     CDS             26470..27579
                     /gene="rpoD"
                     /locus_tag="RD521_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDT7771552.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8990 a         4909 c         5787 g         8101 t
ORIGIN      
        1 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
       61 tctttttcaa tcagtttttt catctgttta cgaaattctt ttttttcagt aaacataaag
      121 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      181 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      241 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      301 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      361 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      421 acaaatgaca actattttct gatttattac aaatgttttt tcaaataaat ttgattacca
      481 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      541 acagcattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      601 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      661 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      721 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
      781 atttaaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
      841 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
      901 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
      961 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1021 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1081 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aagatgcgca
     1141 gcaagaaaca gtcaatgact acgtattgct cgaatcagga aaatcatttt ggttaaatgc
     1201 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1261 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1321 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggaccca tcaatcattg
     1381 ggcagctggt gaattagagt aaaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1441 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1501 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1561 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1621 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1681 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1741 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1801 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1861 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1921 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1981 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     2041 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     2101 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     2161 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     2221 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     2281 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2341 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2401 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt gaggtaacaa
     2461 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtaccat
     2521 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2581 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2641 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2701 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2761 cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2821 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2881 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2941 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     3001 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     3061 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     3121 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     3181 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     3241 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3301 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt tttttttcag
     3361 acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa ggaacaagca
     3421 caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca gagctcaacc
     3481 gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt gaaccagcta
     3541 aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa agatgcaatg
     3601 gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta catcgaattc
     3661 ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca aacaaaaaaa
     3721 gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg agtgattcgt
     3781 gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat cgtgttgtta
     3841 gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc attaaaagta
     3901 gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac ggatatcaca
     3961 agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat cgtaacttac
     4021 ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg acaagttgct
     4081 ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct ggataaattc
     4141 agtaaaaaac taagtctggc tattttggca ttgtctttat tgattttagg tatccaactg
     4201 tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt cagcgcagtg
     4261 atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc tatcgtaaca
     4321 atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat tcggaagctt
     4381 ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac tggtacattg
     4441 acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac tggggatttc
     4501 acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat cagtgtttta
     4561 gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac ggaagtagcg
     4621 tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa caactaccca
     4681 agacaagcag aacttccttt cgattctgat cgaaaattaa tgtctactgg acacacgatc
     4741 gataacgaaa attacttgtt tgtcaaaggc ggaccagata tcgtcttcca acgcagtaaa
     4801 aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa aaaattccaa
     4861 gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc ttaccgtcca
     4921 cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt tggtttgctt
     4981 gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc aagaagtgca
     5041 ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc tatcgcaaaa
     5101 caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact agatgaattg
     5161 acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg tgtttcacct
     5221 gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc agctatgaca
     5281 ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat tgctatggga
     5341 acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga taactttgct
     5401 tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa gaaagctatt
     5461 tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc cttagttgct
     5521 gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt caatgattca
     5581 ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa ccgcccgcca
     5641 agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac gtatcgtggt
     5701 gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca ttcacctgaa
     5761 ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca aacattccca
     5821 gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag aagtgtctta
     5881 ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg actgaaagaa
     5941 gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat gggaatcgca
     6001 cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt acaaggaaat
     6061 aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca caaggtgcaa
     6121 tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga agagttggca
     6181 ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat actgagggtc
     6241 atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg gtcacagtcc
     6301 ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa aagagataga
     6361 atagataaaa gaggagaata atttattccg aaaagtgaaa acatataaca aaaaattaga
     6421 ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta tgaaatccag
     6481 caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac ggagaaagtc
     6541 ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct ttccttagca
     6601 aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga aaatgcaaaa
     6661 aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc gttagaagaa
     6721 tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc agcattggta
     6781 catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt gataatcagg
     6841 gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg aatgatagaa
     6901 gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt aattgatgaa
     6961 ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat tactgaagaa
     7021 ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag gtacattcgc
     7081 aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta tagactgctc
     7141 ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct ttttatgatt
     7201 ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa aaggtacatc
     7261 tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa tcgtttcata
     7321 ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt gtgcgaagag
     7381 atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg caccagaacc
     7441 gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt ccagtagaga
     7501 agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc tgtaacggat
     7561 gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt ggtctaatac
     7621 gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa tcgcctgata
     7681 atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt ggaatgattc
     7741 gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat aatctggacg
     7801 ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg ataaaatacg
     7861 cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt caccaccgct
     7921 tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt ctggatgatt
     7981 tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag cctcttttac
     8041 ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta ccgcactcgc
     8101 ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta gctcacggtc
     8161 tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt cttcatttgc
     8221 acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag gatcatcccc
     8281 acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa aggaaagacg
     8341 atcaatccct attttcattt caacacctct ttacttatga taaaaaactt actttactaa
     8401 ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat tgttaaaatt
     8461 taatcttaga aaaaaacaac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     8521 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     8581 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     8641 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     8701 cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     8761 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     8821 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     8881 cggaacagag agtatgtctc aagcacctat gctgaaaccg tatcagtcag aaacaaatga
     8941 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     9001 acatatggga ttaaccgcag agaaggttgc aacacaattt tctgtgagca gagaagaaca
     9061 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     9121 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     9181 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     9241 aggaactgta acagcaggaa atgcttcgcc attgaatgac ggtgcctctg tggtgatcct
     9301 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     9361 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     9421 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     9481 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     9541 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     9601 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     9661 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     9721 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     9781 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     9841 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatgggcat
     9901 cgcgcagaat tttcaaatca atggtaagaa aaaatggatt ccgatggcaa cagaggaacc
     9961 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
    10021 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
    10081 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
    10141 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
    10201 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
    10261 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
    10321 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
    10381 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
    10441 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
    10501 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
    10561 tagcgcttcg attcacgcct acgcggcaag aaatggatta taccaaggat taacagactg
    10621 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
    10681 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
    10741 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
    10801 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
    10861 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
    10921 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
    10981 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
    11041 aatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
    11101 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
    11161 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
    11221 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
    11281 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
    11341 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
    11401 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
    11461 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
    11521 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
    11581 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
    11641 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
    11701 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
    11761 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
    11821 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
    11881 gggatcaagc atgacttgta acggcaaacc atagcgtcgc aagctctctt cttctagaga
    11941 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
    12001 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
    12061 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
    12121 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
    12181 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
    12241 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
    12301 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
    12361 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
    12421 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
    12481 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
    12541 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
    12601 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
    12661 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
    12721 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
    12781 gctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
    12841 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
    12901 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
    12961 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
    13021 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
    13081 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
    13141 cgacactttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
    13201 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
    13261 ctactaccgg gatttcgtaa ccatttaata gacgataagt gcttgttaat gaattcatac
    13321 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
    13381 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
    13441 tttgattttt ttccgtaaac taagacagaa gggaatgaga aaatggatat ctatttaaaa
    13501 aaagcaattc tgcatattat tgacagagaa actggttctc cagtcttttc acaaaaagag
    13561 ctggatctga caaaagaata tattcgtgat tttcttcaaa agaagatcca aaaaatctct
    13621 tctgctcaaa caaaaactgg acaactagca gaagaaagca tgatcgttga atcacttaaa
    13681 atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg ttcagcggtg gttcgatatc
    13741 taccaagaaa gtgaagaagc acctagtgca gatgtatttg ttgttttgta tgaattagat
    13801 acagtgatgt acttatcttt attgaaagtc aattatcgtg aggcttacac tcactttgtt
    13861 gaagcagacg aagcagggat cgacaaccgc ttgattttga atcgagcaat tctaggggcg
    13921 aaaacacaaa aagcagatga agcattcgct gtgaatttga ctgattggac ctacgaatta
    13981 attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt atttttctgg aagagtgatc
    14041 gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag tgatccaaaa agtagccaag
    14101 catcttggaa aaaaatttga gacagaagaa tttgatatca tggctgattt gaaagaagct
    14161 gtctatgata cgatagaaga aaaaggtcgg ttggatcatg agatgattgc ggaaaaagtc
    14221 ttcaaggaaa acatcacggc aaaaatggct tttaaagaag aagtacaaga gcaaggattt
    14281 gtcccagaag cacctcctgt aaaagaagta caagaaattt ctgagaaaaa atttggaaaa
    14341 caaaaattca agctatcaaa cgggatcgaa ctgatcgtac cagtagatgt atatcgcaac
    14401 ccagatttga ttgaatttgt gaataatccg gatggtacga tatccgtgat gatcaaaaat
    14461 gtggatgaag tattgaatcg tttataaaaa tgactagaac attaggtgtt caagtaccag
    14521 tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa agacgaatat aacaaaaaaa
    14581 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
    14641 gaagcagaag caactccttc ttatttcttg gagttaaacg cttctgtccc agcctctttt
    14701 tatctaataa aattttaaga ccatttacta attatcgatt ttttctgccg catttttagg
    14761 cacttcagaa gcctttattt cttcccagct taatacacct ttacgaggat tgactttgag
    14821 tttaagatac gcattcatct ttaatggatg ttctctttct tcgcgaagcg tcacctgttt
    14881 tttttcacca tctttattat aagccgcttg cttgtaggta taagtcgtga atttctctcc
    14941 gttatctgct tgggtagctg acatttcgcc attcgtcgtg atttttgtat aataagcttc
    15001 gccgccgtaa aaataatgat aagctgcaaa acttccgccg agaaatatgc ctaaaacgac
    15061 aagtacgcca attaattttt tcatgtactt cacctctttc cttattatta acgaaataga
    15121 agatttcgtc tattgaaagt tgttggcaac agctgaattt taaacttttc ctcattttgt
    15181 tcttattgct tcaaaagaaa gttaaggact atcttgaagt ttttctcaaa aaacacattt
    15241 ttttatttat aaaaggtact tttttcgcgt ataagacaag cgagtgctct tagttgactt
    15301 tgaccaatat tgacgtataa taagcttagt tcgaatctta caaaaagtaa gtcggacgta
    15361 ttctttttga tttagtcaaa aaaagactat aataaaaagg tgagagctga aatttttcag
    15421 tgatctatat tatttaggag gtttgtaaat tatggcaaaa gaacattatg acagaagtaa
    15481 accacacgtt aacattggta cgattggaca cgttgaccat gggaaaacta ctttaactgc
    15541 tgcgatcact acagtattag gcaaaaaagg cctagcaaat cctcaagact atgctagtat
    15601 tgatgcagct ccagaagaac gtgaacgtgg gattacgatc aataccgcac acgtagaata
    15661 tgaaacagaa aaacgccact acgcgcatat tgatgcacca ggacacgcgg actatgtgaa
    15721 aaacatgatt acaggggcag cacaaatgga tggtgcaatc ttagttgttt ctgcaactga
    15781 cggtccgatg cctcaaacac gtgaacacat tttattgtca cgccaagttg gtgtaaaata
    15841 cctgattgtt ttcttgaaca aagttgattt agtcgatgat gaagaattga tcgatttggt
    15901 agaaatggaa gttcgcgagt tattgagcga atatggtttc ccaggcgatg acactcctgt
    15961 gatcaaaggt tccgcattaa aagcattgca aggcgatcca gatgctgaag ctgctattat
    16021 ggaattgatg gatacagtag atgaatatat cccaacacca gaacgtgata cagataaacc
    16081 attactattg ccagtggaag acgtcttctc aattacaggt cgaggaactg ttgcctcagg
    16141 tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta gagatcgtag ggatcaaacc
    16201 tgaaacacaa aaagcagttg taacaggtgt agaaatgttc cgtaaaacgt tagattacgg
    16261 ggaagctggg gataacgtag gcgtgttgtt acgggggatc caacgtgacg atatcgaacg
    16321 tggacaagtg cttgctaaac caggttccat tactccacat acaaaattca aagcagaagt
    16381 gtacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcaaca actatcgtcc
    16441 acaattctac ttccgcacaa ctgatgttac aggaacaatc acattgccag aagatacaga
    16501 aatggtcatg cctggtgaca acgtgacgat tgatgttgac ttgatccatc caatcgctgt
    16561 tgaaaacgga acgacattct caattcgtga aggcggacgg acagtgggtt caggtatcgt
    16621 tacagagatc gaagcataag aaacgcgact cgaatatttt ggtaatgaaa aatgtttaaa
    16681 ataaaagagc ggtccaaaca ggatttctgt ttggaccgct ctttgttctt caattgtata
    16741 agaagagatt gtagaagcat gaactttcgt tttacttcag tctcttttat tcatcatgag
    16801 agtgttcatc tatcggtatg cagtttttaa tactttcttc gtatccattt tttcaaacca
    16861 cttattctac cttgtgtctg ctcaactggc gcattatcaa taatcagcaa agcaatcata
    16921 acatatgcaa tccaactatc tctagaatat agagtataac gtgagaccca gtttgtagcg
    16981 tacttgcttt tatattctct taatccatgg aaagaataaa aatgagaacc aaactcgtaa
    17041 accaagtatg caatcctttc ctgtaaaaaa ctctttctgg aagttccgac atttgaaaga
    17101 ggggccatcc ctaaattgaa ccattggata tgttcttctt tcatataggc aaacaaatgc
    17161 aagaacaaga aatccatact gccagaaggt gcttttttag gatcatatcg catcagatca
    17221 atcgttccta ctttattgtt cgtgtaagta ggcatgatcg ttgcaaaagc gacaatttct
    17281 tcttgggtat ttttgacaac tgcaataggc gctctttgta gataatcttc agagaaaaag
    17341 cctaaagaaa accctttttc ctttcgtgaa cccaaccaat tatcagagat atttttgaaa
    17401 gtactcattt gctcagtaga aaatggtggt tgtaagacat caaatgtaaa tccttctcgt
    17461 tcgatccgat tcaagacagc acgagtgccc ttcatttttt tccctgaagt tgtaaaacta
    17521 ttcaggtcta cataagcttc ttcccccatc ttaatgaaat cgtagccgaa ttcatgcaaa
    17581 atcataacaa tctcttcgct tgtttcgtag aaaactggta aatagcatag tcgatctgtt
    17641 tcttcgataa aagcttcgat tgcttctgga aaatcttctt tcttaccaga aggatcaccc
    17701 attacaatac atttattatt gaagcaagcg aattggagaa gaacagttgg ttcaccgtct
    17761 ttttcatagg caaacatccg cttatctttt aagaagatca attgactatc ggagtttcct
    17821 ccataagttg taaggatctt cagggccttt tcttcattaa aggcttcgcc aatctgtttc
    17881 ttttctcctt gaaggaaatg aacaaataga acgatcatga aactaacagc gatgatggcc
    17941 aacaatcccg aaaaccaaat cttttcagaa ggaaataaga aaaaggaaat aaaatggtga
    18001 cgccgatgag ggaaatccgg cagattgtat accccaataa cgatgtaaag caaggacagt
    18061 gcaccaataa tgattccatc gatcgtacgc cactcccatg aatagatcaa ttgttcccga
    18121 aataattcgt ttttagaagc aagaatgatc agaagcagaa tagacaagaa aaccaccggt
    18181 gtgaaactaa aatcacttaa taaaacataa agtaaagcta gaacaatcaa gaagattgta
    18241 ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc ccataatgat taaaagaaat
    18301 cccaatagta tactaggaaa ttggataata aaatgaaatt tcaatgggtt caaactatga
    18361 agccagcgaa actctgtaaa agcttgcggg atagtggcag aaagtactag catgattcca
    18421 gaaaagtata ataagacaac cacgatttta tgcgcaattt ctgttgctaa ttgtttaggg
    18481 ataccagaat aacgctgatc aaaagtggaa ccgatatttt tgaagaaaaa gacgattcca
    18541 atcaaaaacg gaatgatgta gtagaacagg cgatagagca agatccacac aacgactatt
    18601 tctcttggca caccaatagc ggacaacccg ataatcatca ttacatcgaa actcccaatt
    18661 tctcctggaa tcatagacac gattcctatc actgaagcag caatgaataa cggaattgtt
    18721 tgccacagat caatccggat ttccattaaa taaccaacag aaaggaaact aaacaggaca
    18781 ccggtccatt ccaacaatga aactagcaat agctgtcctt gagttttaag tgaggtacca
    18841 ccaaggaatc ctttcttttt gaaagtagta aataaaaaga cgacaggaaa atatagaccg
    18901 ccgccgatca accatatcca atattgcttc aaaaaaggag caactggcgt gaagactaca
    18961 agtaaaaaag aaagcagact gtaaatagaa agtccagcca tcaaaaacaa gaaaactttc
    19021 gataatgctt ggatcacgtt tctgctctct ttcttttgtc catatagttc agagcgaagc
    19081 cccacactaa caaacccgcc gaaaccggca atattgttga ttgtattgat cagccagctt
    19141 gtctcaaaca agtaacgagg attctgcttt tgttcaagca tccgattcaa aataatatca
    19201 taaccaatca taggtaacac agagacaaga ccaatcaaca gcataagtcc gcttttccat
    19261 acaggtatag tggcaaacgt ttctcctaat tgctggatag atagagtttt accgatggac
    19321 atcaattcac cgacaataat gacaatgaca gaaatcaaaa agattgtttt gaacagtcct
    19381 aggtggtttt tcagccattg tatggtattt tttaacaaat aaatcacctt ctattcctaa
    19441 tagttatgtt tcagaaagaa attgaagaac ttctcgattg aagcgttcag gatcttttct
    19501 agctaattca tgtccttgtt ctttcacaag cacaaaggaa gcttttggta tagttttagc
    19561 tatatacaaa gaatgtttta gcttgattac atcttttttt cccacgatga tcaaagtggg
    19621 ggagttgatt ttttttaaat cattttctgt aagcccgatg tcatgcagca aaagtttgat
    19681 aactaaaaga tttttgcgaa gacttgggcg aaataaagag agtaaccata cccatgcata
    19741 atgaatatta ctgatgactc tggcagaaaa acgaacgcct ttaacaagcg tgtttccaga
    19801 atttaaaatc aatcgatgaa cttttttagg gaagctgctg gcaaatacca gtgctagatt
    19861 tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt ttttccagca acatgatcgt
    19921 attcagatcc tcggccatta actgaaaatt caacatagat gcttcgtttg tcgaacgacc
    19981 atgtcctcgg ctatctacta gatacacggt atagtagcgt tcgagtacag ggacctgttc
    20041 tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa aagagaggga agccttgacc
    20101 gctcttttca tagtaaattt cgctcccatc tggcattttt gcatatttct tttctttttt
    20161 cacataatca cctactgtgt aaagtataac gattatatca gtcattgggc aattgttttt
    20221 actgaaaaac aacgatcatt ttcgtaagat gacaaaaaat acaggaaaat tcattttttc
    20281 ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa gataaagaaa tagtaagata
    20341 aaaaaagatg tcaaaataac taggtgatga aatgaagcga aaacgattat ctattatccg
    20401 tattcttttt tacatgatca ttctgacggt tattggtgcg gtgttgtttt taggaggaag
    20461 aacttatcgt tcggtaaaac gagtggaaag ctatcaaata gaaataacaa cagctgcaaa
    20521 aaattatcat atggaaaact acgagaattt gatcaaggcg ataatattga ccgaatcaaa
    20581 aggaaaaggg acagatctca tgcggagctc ggaaagtctg catggagaag ctgagatcat
    20641 tgataatcca gctgacagta ttgatcaagg tgtcaaatat ttggcggaaa tgatagtaga
    20701 agcaaagaaa caaggatgcg atttatggac agctgtccaa gcatataatt ttgggaaaga
    20761 ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca gtagaattag cagaagcgta
    20821 ttctaaagat gtgctgagcc cacttttagg aaatgaggaa gggaaacaat accgatattg
    20881 gcggatccag tccttgcttt acaatggcgg ctatctctat cataatggag gtaacatgtt
    20941 ttatcctgat attgtcaaaa tgaatgaaaa aataatagag acctatgaaa aagtctttaa
    21001 gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa tgaaagaagg agtgcgcaaa
    21061 tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg gtacacaacc aaagactgga
    21121 tgttacggtt tgtgaaggga atgtttatcg gttcaggatt tatcttgcca ggagtcagcg
    21181 gtggtgcatt agcagctgtc tttggcatgt atgagcggct gatttctttt ttagcccacc
    21241 ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc tgttggtcta ggcggagtga
    21301 caggcatctt tctactttct tttttagtca gttttttatt aggtgcgtat gaaacaacga
    21361 tattatggtt ttttgtcgga tgtatcgctg gaactgttcc tgcgttatgg aaagaagcag
    21421 ggaaaaaagg aagagatact tctgatgtga tcatcatggt tgttactttt gttctggctt
    21481 atttgttttt attatacggc gctcaattat ttgacgggca agtcgatcag aattttggaa
    21541 catggctgat tgccggcgga ttgattggtt tgggtatgat cgtcccagga ttgagccctt
    21601 ctaatttttt ggtgtatatg ggattgtata aagcgatggc tgacggattt aaagaagtac
    21661 gacttgaagt gattgtgccg atcggaattg gtgggattct ttgtgttctt ggattgtcaa
    21721 aaatcatgga ttacattttc tcaaaggcat atagcaagct attccatttc attttaggtg
    21781 tagtttttgc ttcaacgatt atgatcattc ctaccgatta cgcgggattg ggagttgctg
    21841 gggtactagc ttgtcctgtt ctttttatag ccggtacagc actaggttgg tggatgagcc
    21901 gtttggagga acaatataag taaagataga ttgataataa aaacagcaca cgggtgattc
    21961 aaacaaaaag gttggcatct tttttgtaaa atcattcgtg tgtttttcta taaattttat
    22021 aaaaacaagt tgtttttaat cttggaacac gctagactta aacacgtgat tatttttgaa
    22081 agttggcgga aaatggagaa aaagaaaatg aatggactta cacagcaaga ggtagttcag
    22141 aaaatggctt ctggccagca aaatgactac caggaagatg catcaaaaag tacaaaacaa
    22201 atattcagcg ataatatatt gacgttattc aattttctga actttggtat tgggatttgt
    22261 ctgttatttg taggtgcgta ttccaacatg gcgtatttag caattattgt cgtcaatatc
    22321 gttattggaa tttatcaaga aatacatgca agaaatcttg tacggaaatt atcgattgtt
    22381 tcccaatcag aagtactagt gatccgggac ggtaaagaac aatcgatttc tactaaagag
    22441 ctggtattag gagatttggt aaaaatcgga gcaggagaac agataccagc cgatttacag
    22501 gttatatctg gacgggcaga agcaaatgaa gctcttttga caggcgaatc tgatttgatt
    22561 gtcaaagaaa ctggagctga attgctttct ggaagctttt tgacaagcgg ttctgtcatg
    22621 gctgaagtta tccgagtagg tgcagataat tatgctgtcc gcttgactag cgaggcaaag
    22681 actcacaaag ctattcgttc tgaacttgta gattcgattc gcaaagtatc caaattcact
    22741 agtttcgtga ttattccttt ggggatcata ctatttttgg aagcactgtt catccgcgat
    22801 gcttctatca aaatttctgt tattgcatct gcagcagctt tgttaggcat gcttccaaag
    22861 ggtttggtat tgctaataag tattgcatta acgacagggg taaccaaact ggcaaaaaaa
    22921 cgaatccttg ttcaagacat gtattcaatt gaaacgctgg cccatgtcga tacattatgc
    22981 ttggataaaa cagggaccat tactgaaggg aaaatgagta tccagcgggt agaagcacta
    23041 catccgctgt acgagaaagc catcccagaa attatgggga cgtatctggc agaaagcagt
    23101 gataacaaca tcactatgcg ggctatgaga gattattttg agctttctaa tcggtatgat
    23161 gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg gcgcgatgga atttccagaa
    23221 ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg aagaagatcg gcttccggac
    23281 agtctaaaaa cagcccaagc gaatggctat cgcgtactta tgttggggat cgctccaaat
    23341 cagcaattga acgaagaaaa accagcattt gttgagccgg ttgctctgtt tgaaatcgat
    23401 gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc ttcatgagga aggtgtggat
    23461 ctgaaagtga tttctggaga caatcctttt actgtctcca atatcgctag acgtgcagga
    23521 ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta ctgaagaaat ggaagtgcga
    23581 gaagctgctc atcgctatac ggtttttgga cgagtgagtc cgcagcaaaa gaaattgctg
    23641 gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga caggcgacgg tgtgaatgat
    23701 gttttggcgt taagagaagc agattgcagc attgctatgg cagaaggtga tggagcaaca
    23761 cgtcagattg cgaatctggt attgttggac tctgatttta ctacattgcc agaagtcctg
    23821 tttgaaggac gccgagtggt caacaatgtg acgaaagtct caggcatctt ttttatcaaa
    23881 acgatttatt catttatttt atcgattata tgtgccgtta cagcaattgg atttccgttt
    23941 atccctatcc aaatcacgct gattgattta gcgattgaag ggtatccttc cttcttttta
    24001 tcttttgaac aagataaaag aaaaatcact taccgttatt tgcctacagc acttattaat
    24061 gctttaccaa atgccttgct tgtagtagtg aatatcattg cggtgtatct acttggaaaa
    24121 acattcgcat ggggaaatgc tgaaactact actttgatgt attacttgct gattgggatc
    24181 agttgcatgg cagttgtaaa agcttgttat cccttcaacc cattgcgcat cttcctagca
    24241 gtaacgacga tcgtagggat atatgtagct gcaatgctgt tccatggaat tttggaagta
    24301 aacttagggt tgggagagag cgtaggatac ttctttgtat tcatgctcgt aaatattgtt
    24361 cttcgagtat tgtactggaa ggctgatatt caaaatcagt tactgaaaag agtagcaaat
    24421 tgatgcttaa tgagatagaa atgtgttata cttgaaaaag caatgaaata tgatacaatc
    24481 aaacatgtat aattgtgtcc tttaaaatcg cactgatttc agttagtgcg attttttacc
    24541 ggttattctc ctgaaatatc ttctgttaaa cccaaaagat atcaatagag cagggggtga
    24601 aaaaatggct caacggattc ctcaagaggt tatcgaaaca gtacgcagtc aaacgaatat
    24661 cgttgaggtg attggacaat atgtccagtt gaaaaagtct ggtaaaaatt acttggggct
    24721 gtgtcctttt catgaagaac gtacaccgtc tttttctgtt gcagaagaca agcagatttt
    24781 tcattgcttt ggttgtggta aaggcggaaa cgtgtttact tttcttcaag aactagaagg
    24841 tctaagtttt ccagaagcag tggtaaaagt ggcagacttc gaacagatac cgttagaaga
    24901 tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa tctgctacgg ggaaactgat
    24961 tgctgctcat gaaaaagcag cggaaatcta tcatcacatg ctattgcata caaaagctgg
    25021 acagccagct ttagaatatt tgcaaagtcg tgggttgtct ttagaattaa ttgaagaatt
    25081 caacataggt tttgcaccta atgagcgttc ttttcttcag caagtattca agaatgaatc
    25141 aatttcaaat gaattgttag aacgctctgg attgtttgtc acgcatgatg atggccgtat
    25201 gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt aattctcaag gccagacgat
    25261 cggtttttca ggacgctggc ttgcacctga aaaagggcag gaaaaagatc agcccaaata
    25321 cttgaatagt ccagaaacag aactgttcaa taaaagacaa gttttattta atcttgataa
    25381 agcaagaagc gatatccgga aaaacggaga aattttgttg ttcgaagggt ttatggatgt
    25441 gattgctgca tggcaggctg gtatcaaaac tggattagct tctatgggga caagtctaac
    25501 agctcaacag atccaacaaa tggaaaagct gacgaaagaa ttggtctttt gctatgacgg
    25561 agacaacgca ggttttgaag caaccaatcg agggattgaa ttgcttcggc aaaacagtcg
    25621 attagcactt tctgtcgtag tcgttccaga aaagcttgat cctgatgaat atctccgtaa
    25681 gtatggagaa gcatctttcc aagaattagt ggctcatggg cgcgaaacag tctttacttt
    25741 taaatcacgt tatttaaaac aagggaaaaa tttagataat gaaaaagaca agatcgaata
    25801 cctcgaagaa ttactagttg aacttagccg agttgtttca ccaatcgaac aagatatgta
    25861 tctatctcaa ctgtcaacag agtttcagat cggcagagag acgatacaaa aacaactgcg
    25921 cgatcttcga aggacaaatc agcaaaatcg tcaagaacct caggaaagtc aacacactca
    25981 ggttacgaga caagaaactg cacggaaaaa gagacctttg acgcaagtag aaaaggcaga
    26041 gcagattcta ttatatcgtg catttaaaga agtttctgtc cgtaatattt taaaagaacg
    26101 agaatttcag tttattcatg atgcatatgc agaagtttat tttttattcg atgcatatgt
    26161 ctcacaacat tatgaattca accttgcaga gtttttagac tttttgcaag atgaaaattt
    26221 aaggcggttg gtggtggaaa tcgaatattt accggtggca gaagaaagtt cgccacgaga
    26281 aatccaagac ttgctagatg tgatcagcaa atctggctta gctgatgaaa taacattaaa
    26341 aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa cagcttgagc ttgaattgac
    26401 gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc aaatagctac tcaagggggg
    26461 ccttctttta tggcaaatgg aacagataaa aaaacgtatg aagcagcagt agcagctttc
    26521 atcaaagaaa acaaacccaa acgacaagtt gtatatgatg atttatccaa tcagcttgct
    26581 acaccattca ctttgaatgc agatgaaatg gacaaattga tccaaaagat tgaagacgca
    26641 ggtatcagtg tggtggatga aaatggtgat ccatcaattc acagcttgaa gagtgctgag
    26701 aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc caaccggtgt aaaaatcaat
    26761 gacccggttc gtatgtatct gaaagaaatt ggccgtgtct cccttttaac tgcggaagaa
    26821 gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag aagcgaaaca gcgtctggca
    26881 gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt atgtaggacg aggcatgcag
    26941 ttccttgact tgatccaaga aggaaacatg ggactgatga aggcagttga aaaatttgac
    27001 taccgcaaag gatttaagtt ttcaacctat gctacatggt ggattcgtca agccatcact
    27061 cgagcaatcg ctgaccaagc acgtacgatc cgtattccag ttcatatggt tgaaacaatt
    27121 aataaattga tacgtatcca acgtcaattg ctgcaagacc ttggaagaga acctacgcca
    27181 gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag tccgagaaat cttgaaaatc
    27241 gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag aagatgactc tcacttaggt
    27301 gatttcatag aagaccagga agcaacaagt ccagcagaac acgcagctta tgagttacta
    27361 aaagaacaat tggaagatgt actagataca ttgactgatc gggaagagaa tgtccttcgc
    27421 ttacgttttg gtttagatga tggccggacg agaacattgg aagaagtagg gaaagtgttt
    27481 ggtgttactc gggaacgtat tcgtcagatc gaggctaaag cattaagaaa attgcgccat
    27541 ccttctcgtt caaaacaatt aaaagatttc ttagaataag cgtggactat tgaccagaag
    27601 agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa aagggctctt tgtcaataag
    27661 gactgatgag ttgtgcaaaa tcaaatctga gtcagaatga accacattct ggctcagatt
    27721 ttttgttatc ttacattgat taattgattg atcaaaaaga ttctaattct catgttctca
    27781 aatgatt
//
LOCUS       DAPBCS010000037        27531 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPBCS010000037 DAPBCS010000000
VERSION     DAPBCS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.037"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..888
                     /locus_tag="RD521_001840"
     CDS             <1..888
                     /locus_tag="RD521_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDT7771553.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
     gene            complement(930..1622)
                     /locus_tag="RD521_001841"
     CDS             complement(930..1622)
                     /locus_tag="RD521_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7771554.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            1617..1883
                     /locus_tag="RD521_001842"
     CDS             1617..1883
                     /locus_tag="RD521_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7771555.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(2059..2628)
                     /locus_tag="RD521_001843"
     CDS             complement(2059..2628)
                     /locus_tag="RD521_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7771556.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2702..3991)
                     /locus_tag="RD521_001844"
     CDS             complement(2702..3991)
                     /locus_tag="RD521_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771557.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            4345..5193
                     /locus_tag="RD521_001845"
     CDS             4345..5193
                     /locus_tag="RD521_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDT7771558.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5338..6279
                     /locus_tag="RD521_001846"
     CDS             5338..6279
                     /locus_tag="RD521_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDT7771559.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6269..8440
                     /locus_tag="RD521_001847"
                     /pseudo
     CDS             6269..8440
                     /locus_tag="RD521_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8552..10723)
                     /gene="copB"
                     /locus_tag="RD521_001848"
     CDS             complement(8552..10723)
                     /gene="copB"
                     /locus_tag="RD521_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDT7771560.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(10743..12929)
                     /locus_tag="RD521_001849"
     CDS             complement(10743..12929)
                     /locus_tag="RD521_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDT7771561.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12929..13138)
                     /gene="copZ"
                     /locus_tag="RD521_001850"
     CDS             complement(12929..13138)
                     /gene="copZ"
                     /locus_tag="RD521_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDT7771562.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13151..13591)
                     /locus_tag="RD521_001851"
     CDS             complement(13151..13591)
                     /locus_tag="RD521_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDT7771563.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13665..14204)
                     /locus_tag="RD521_001852"
     CDS             complement(13665..14204)
                     /locus_tag="RD521_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HDT7771564.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(14819..16285)
                     /locus_tag="RD521_001853"
     CDS             complement(14819..16285)
                     /locus_tag="RD521_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7771565.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(16596..18677)
                     /gene="cadA"
                     /locus_tag="RD521_001854"
     CDS             complement(16596..18677)
                     /gene="cadA"
                     /locus_tag="RD521_001854"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDT7771566.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(19202..19561)
                     /locus_tag="RD521_001855"
     CDS             complement(19202..19561)
                     /locus_tag="RD521_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDT7771567.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGISLIVNIIWLIISGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(19591..19932)
                     /locus_tag="RD521_001856"
     CDS             complement(19591..19932)
                     /locus_tag="RD521_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771568.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSFFAQF"
     gene            complement(19929..20603)
                     /locus_tag="RD521_001857"
     CDS             complement(19929..20603)
                     /locus_tag="RD521_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7771569.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLIAQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(21533..22831)
                     /gene="asnS"
                     /locus_tag="RD521_001858"
     CDS             complement(21533..22831)
                     /gene="asnS"
                     /locus_tag="RD521_001858"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDT7771570.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22871..24061)
                     /locus_tag="RD521_001859"
     CDS             complement(22871..24061)
                     /locus_tag="RD521_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7771571.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(24082..24609)
                     /locus_tag="RD521_001860"
     CDS             complement(24082..24609)
                     /locus_tag="RD521_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDT7771572.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(24656..27445)
                     /locus_tag="RD521_001861"
     CDS             complement(24656..27445)
                     /locus_tag="RD521_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDT7771573.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
BASE COUNT         7833 a         5782 c         4843 g         9073 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt caacttttgg
      901 ggttcagttc acaaagaggg ggggattttt tatcgttgtg gaatatttaa ataccaacgc
      961 ttgtcatgaa aatcttgtgc tccaccctta gtatttcctt ctggatcgtt tgtcgcacgc
     1021 atcatgacgt atactttctt attagggaaa tcacgcatat tgaaagatac atgatagcca
     1081 acatttccta aagtattata agcttgattt acgtctggtc tataaatccc atcagctctt
     1141 actctagcta gttcttttcc agtattgtag tccataatga aaatatactc gtatttataa
     1201 ttagcaatgt gccatccagc tacatgcaag tttgcgtttt cgatttctcc gaactgatca
     1261 atgtgggcgt aatttgttcc atctgtcagc gtagaatttg cagcacctgc tcgagttgga
     1321 tcaattactg gtttatcctc cgaagtagtt ggattatcat ccgtaaaacc atgagccaaa
     1381 tcataggcta gtttttcttt acttacaccc atttcagaaa gataaccgta aggatctgta
     1441 tgatcgcccc aaatattttg tgttacccat aaatgcgatt tgattcctgg ttggttataa
     1501 ggagtgtcta atgttaatgg aataccatat ttcattgctg aatctcttgc caattcaacg
     1561 tatactttat agttcttttc aaaagtttct ttatcatgtg tgtgttgtaa ctcgatttgc
     1621 acaggacttt tggctcacga ggttgtccta ctctacatct tgctgataga tatgctgaaa
     1681 ggaaaacacc aacattatgt tttgcttttt cagctagaac gagacaagca ggaatacctt
     1741 tagaagatat caaagatttt ttaggtcata aagatgtttc cactacacaa gtctatgccc
     1801 atatttctcc cgaagtgaaa aaaagatcaa tgaatcaact agaaaactat atagaagagc
     1861 aaataaaaaa gcactcaaat tgagtgcttt tttaagctca ctatcactaa aactatcact
     1921 aattttattt ataagcgaac atgtttctat cactgatttt ttaaatctag cgaaaaaccc
     1981 ttatcttaaa ggcgttcgtt caattctttg gcaaggtctt caaagcctgg cttgcctaga
     2041 agtgcgaaca tggttttctt atagctcatg tagaaaaatt ttcttgatga tgattttatc
     2101 catcaattga gattgactgt tttgctctcc tatgatttca gctatgattg tccgattttg
     2161 cggttttctt cttaactggt agttgatttt cccagtttcc atcaactcgc taagcatatc
     2221 cacgactccg ccaaatccgg tctttttcgt aataaaacct agtgctacca ttcgttcatc
     2281 tagatttttt gctgtaatcg tcacaccagt aatgagcgag tgctgtaatg tttgggccag
     2341 ctcttccatc ccttcagcat tggatacgac ggaagctgtt ttgttaatag gttcttcgtt
     2401 tatttctttt ggttctttcc tctccacttt cactgtttga cgaaggacat ctagttcttg
     2461 caagagcgtt ttgtattgtt cttgggtcag catcacgcca gctacttttt cacgattaaa
     2521 tatatatacg ccagttcccg cgtctcgcgc ttgagcaaat acatccatcg gcgatcgttt
     2581 cacttctgtg atcgaagatg tgggaacttc taatttcttt aattccaatg gatttcctcc
     2641 tttactaatc gctttttatt atagcacaga ctgacttgtt ttgatctttt ttccaaaggt
     2701 tttatcggtt tttgcctaac cagtaattga tatcggtaag aagtctttcg ataccaaatg
     2761 aaggatggta cagatacgtt ttttgatctt cgttatggtc tagtggaaaa aaatgatcgg
     2821 tgatcactag atctgcatac cgaggcggct tatttcgagt acatacatgt atagagatcc
     2881 gttttcgatg tcttagcgtt ttgttcagta ccccagcaag tcttcttgct tcttggtgtt
     2941 catagcgaaa aaaaaagacg attttcactt caggagggtg gacatctaaa taccctcgtt
     3001 cctggagtgt tttcagtaaa agcatccccc actctactgc ggggtgtgca agtgcttgtt
     3061 ggatcgctag ccgaaattga tatagtttat ctttggaatt gggaaatttt tctaatgttt
     3121 cctgataata acgaacttct tctatcagcc ggtcatggat accaattacg taacgaagcc
     3181 attcttggta aagtactttt atcagctttg atttgctttc gctctcaaat tcgctattca
     3241 gccaaaacag cgaatcaagc ttttcagtca caatcgtcaa tttccgatag tcttgtgtct
     3301 cttctatccg tttccaccgc ttttcagtaa tctggttaca gctcaagcgc aaaattgcta
     3361 gataattttt ttcttttatt ggcaaaatct gctcctctct aaatagcaat tcaggaaaca
     3421 attcaaagaa tagtgttcga ctgtctccta aaatctcgat ttctgccgga tcctttcttt
     3481 gcttcaataa ttggatgaac atctcagaaa aggactgctc tccatcatct gcaaaaaaat
     3541 gcggaaaatc tttcaaaaag taaaacagca gatatcttac ttttctttct gaaccaaaga
     3601 tctgtgcttt tttataagaa aaagaaaggt ggtactcgcc aaaccacttt ctcaatcgct
     3661 cttgcgtaga acgatcgtac ttctgctcgt aaggttcatt taatagcaga tttttcagta
     3721 aacaaaacgg gggtgattgg atcagaaaaa caaacaagag ataaggttcg ctatatcctt
     3781 gatcataaac taactgaacg gttttcttat gaagaatcag ctgaatggga cagtcgcttt
     3841 ccgccaaacg tttcagatgc cgatccactg ttttaacatc gattttcagt tcttcagcta
     3901 aatcagcttt tcgccagatc aaatctttca ttagattgat tagtgaaaat aaattctgtg
     3961 cattttttga taatagtttt tgccatctca tcgatcattt ctcacctgat ttctaaacat
     4021 attgtttgat caaaatgagt tcactcctaa aaagataaaa aactcccttt ctatttattt
     4081 aaatacgcaa atcttcctca ttatttttta ttttactcaa tttaatcgta ttctcatttc
     4141 aaaacgaatg tactttaaaa aaacataata agatcacttt gcttgtagga tgtattccct
     4201 tccattacaa tgaaataaat agcgatttta tgagaaggtg atgtgaaaat ggaagaaaaa
     4261 atagaagaat ccaaacaaaa aatcgatcag ttgatataag aattagaaaa agcggtcatc
     4321 ggcaaacgtt caactatcca attgatgatc gtggccctat tagcaggtgg tcatgtctta
     4381 ttcgaagata ttcccggtgt aggtaaaacg ttactggtca aagcactagc aaaagctgta
     4441 cagggaacct tcagcagagt ccaatgtact cctgatctgc tgcctagtga tattcttggt
     4501 ttttccgtat acaattcaca atcaaaagag tttgaatttc gtccgggacc gatctttacc
     4561 acgatcttgt tggcagatga gatcaatcgt acgactcctc gtactcagtc agcactttta
     4621 gaagcaatgg ccgagaatca tgcaacgatc gacaacaata cataccctct agacaatcat
     4681 ttttttgtct tagctacgca aaatccaatc gagtacgaag gaacatatcc tttgccggaa
     4741 gcacagctcg atcgtttttt attccgacta caaatcggtt atccttcttt tgatgacgaa
     4801 cttttgctac ttttggataa attcgaaaaa gtgttggata acttaaatga agtattgaac
     4861 agttatgaaa tagaagaaat aaaaacaaat gtggcgaatg tctttgtcag tcccgaagtg
     4921 gcatcttatg ccttgcagtt agtgtcagca acaagaagtc atgctgccat ccagcttggc
     4981 atcagtccac gaggaagcct ttcctttatt caggcagcaa aagcttatgc tttaatccat
     5041 gggagaaatt acgtcacgcc caaagatttg caggatttag ttccttacgt gttttcccat
     5101 cgcctgattt tttacgatcg ttcattgaag gaagacgaaa agaaacaaat cattcatact
     5161 cttgttgcac aaattcctat tcctgtgagg tagattgagg tagacaatgg aaaaaatcaa
     5221 agattatttc attaattttg tgttatttct ggtatatttc atcattgtgc tttttgctct
     5281 tgtattcaac aattctttag gctggttctt gttggccttt tttactcttt atgtatcatg
     5341 gagcttgatt tcattaagca gctttctaaa gaaactttcg gttacttgta cattgcagcc
     5401 actcgtttat cgcaaaaaaa gtatgcaggt tattttttcc atagcaaaaa agagaacttt
     5461 ttctttacct ctccctcgtt tacaaatcgg cttcccagca atctttgcag aaaaaaacaa
     5521 gagattttga ttcttacaaa taaaccgcaa gaagtccaat tgctttggca gccaaaagaa
     5581 agaggatgcc atgaaacatt aactgtcatt tttacagcct atgatagtct aggacttttc
     5641 agaaaaagtg caaaaagaga gatctctttt cctgtgactg ttctgcctgc tttttgtaag
     5701 attcaagcag aaaaattaca gagagttctg gaaaaaaaac agcagctgaa cgcgtaccaa
     5761 agaggtcttg attttcggga acatcgcgct tatcgtccag gtgattcttt caaccgaatc
     5821 gattggaaat tgtctgctca tactcaagag tggttatacc gtgaatacga ttatcaagaa
     5881 gaagaatgtt ctcctctttt ttgcttttgg ggttcgccca gtccaaaatt tgaacctctg
     5941 ctagacttgt atttttcttt atggcatttg agaaaagaaa agcagccgga acttctgata
     6001 ttaggcaacc gcgcctttca tggcaaagac cccggtcata cctattttgc ttctttggaa
     6061 gctggcgatg agaaagcttt tttcgcacac ttgaaaaaat atgcaaaaaa acaaatcgtc
     6121 ctctttgttc ctgagaattc tcctgaagtc tcagaagcta ttgaaacatt gtcaaaagat
     6181 cgaacggctt atcttgttta tttcgtagaa aaacaactca tggtccaaga aaaagacaag
     6241 atatgttcat tgccaggagg tgaatggatt gacgattaaa cgggaaaaat ggctgcgtgg
     6301 cgtttactcc tttgtgatac taagtgtagg tgctccgata tttgtagtag ctaatcaatt
     6361 gcaaaacagc taccttctta tcctaactat tggtgtccta tgccttattg tctgctgtat
     6421 tcctaaaaaa tgggtacgtt tgctgctttc tttgccagta atgataggtt gtttgttttt
     6481 atattttcct tcgaagacat tttccatatt atggatctta cagttttttt cgtcagttac
     6541 agaggaattc ttccatctat cccaaggtaa actttattat ttgccagaaa agacagcttt
     6601 ttttctcata atgttgctga tctatttatt tattacattg accgtggatt acgactattg
     6661 gtacgcaccc tttctttctt tgatggctta cttcatgatg cttaccgttt ttcggaaaaa
     6721 agatttactg tacgaaatga ttgcggtagt aactgttttg ttcgtgtatc tggcagcaag
     6781 gcaatttttc tccatccggt tgttgtcggg aaacactcga ttccagtcat tgactacagt
     6841 tctgcttatt tgtttcttaa cttttagcgc tatcttgcct ttatatcttc cgaaagtaca
     6901 ccaatctctc gaggaaacga gtgaaccgat ccgagaatat cttaatgatg aagggcttta
     6961 tgacacactc catgagtatc aattaaccgg aagtgcaaga acaggcttca gtgagaatga
     7021 tgaacagctt ggtggacctt tatatgataa tggcgaagtt gtttttacag cgaaccaaaa
     7081 aggaaatggc tattggaaga tcgaaaataa aaatcattac accggttctg gatggataga
     7141 agatcaggac gcacccatcc aaccaatcca agaattccct gttgacatca cgagctacca
     7201 aaccgttata gaagacaaag aaaacattac attgacccta aaaaaacaag ctttcttacc
     7261 ttatccttat ggaaatacta ccttgactga tttttcatta gctagtggac aaacgcttta
     7321 ctatttgcct gaaagccagc gattcattcc tgtcccgaat gaatcgacac gttctgcaac
     7381 tttacaaaca gaaaggttag tttttacgag agaagaatta aaaagcagtc agttaaaagc
     7441 tgatgattta gaaaatcagc agacaaatct tcaattgcca aaaagtttgc ctaaacgtat
     7501 tgaggacttg gcggtcgaat tgaccaaaaa tgaagcaact ttaattcgta aagtcgaagc
     7561 tgtctaacag tatttaaaaa caagcggaaa tttccgctat tccaaaacgg ataccgggca
     7621 tactccgaaa gaacacgatt atgtggatta tttcctcttt gattcaaaag tcggctattg
     7681 tgacaacttt tcaacttcta tggtcgtcct tcttcgaaca cttggtatcc cagctcgctg
     7741 gacaaaaggt tttacaaatg ggctcagaac aggcacagat aatcaaggga atgaggaata
     7801 cagcattctc aatagtcatg ctcattcttg gccagaggta tactttcctg gatttggctg
     7861 gcttccattt gaaccaaccc cttcatttgc caatccaatg ggttcacaac cagttgctga
     7921 aacaaccgaa accgtccagc ctgctatcga acaaccaaat gaaagcgaaa atatggcaga
     7981 aactatttcc agtgagagat ctgaggtcat tccatcagaa gaaacaacac tcacaccagc
     8041 ttataaaatc gtcttattca gccttggagt gtgtttatcc gtcttgttgt tctttctatt
     8101 gttattcaga aaatctctgc gctggacaag ttatcttatc tatcttcatt tttttgcttc
     8161 tactaatacg attgcatatc aaaaacttct taaccctttg gaaacattgg taccgcgcaa
     8221 acccgcagaa tctttgcagg aatatagcct gcgaatagaa caagaattca agctagatct
     8281 gcgtttcatt cgattaacta ctcaatatga agcagaaata tatggacgac aagcagatcc
     8341 ggtaaaattc gataaaaaaa cctatttaga aataacgcgt ataataagaa aaacggtata
     8401 caaaaaaatt ctgacaaaag ggagccgctt caaaaaataa gataaaattt ctggagaatc
     8461 tacttctgga atgccgagaa ccagctgatt gagtatcgat tactcgaaaa aaaggttgtg
     8521 acacgttttt tgtcacaacc ttttttcatt attatttgat tttcaatgtc aaagcattta
     8581 gagctacaac taccgtactt aacgacatca agacagcacc aactgctgga cttaatatga
     8641 taccaattgg cgcaagaatt ccggctgcta gcggaattgc aagaatattg taacctgccc
     8701 cccaccagag gttctggatc attttctttc gcgtttgctt ggctagatca agaaaatgca
     8761 aaatatcttt tggatcgctg tttgtcaata cgacatccgc agaatcgatt gcgatatctg
     8821 tccctgcacc gattgccatc ccgattgaag cacgggctaa acttggcgca tcattgatcc
     8881 catcacccac catgattact tttttccctt ggtcaaggta tttctggaca acagcttctt
     8941 tatcatctgg taataaaccg ccataatatt cttcgatccc taaataatct gctactgctt
     9001 gggcagcgtt tttattgtca ccagtaagca tgacaggagt gatttttctt tctttcaatg
     9061 ttcggataaa ttcttttgct tctgttttta ctacgtcacc taaagcaacg attgcagcta
     9121 gatgattttc taaaatcagg aagctgatgg tatttccttg ttcttggtaa ggtttcaata
     9181 atgcttgttc tacgtttaat ccgagacgct caacttcttt ttcattaact attttcactt
     9241 gttgtttttt gactgttgcc tctagcccga ctcctgaaag attttttaga ttttcagctt
     9301 gataaggctt gatttcatgt tctttcaaat aattcataat gccaacagcc aatggatgat
     9361 tggcattcgc ttccaaggct cctagatatt gaaggatttc atttttattg aacgcatcat
     9421 caagtacttc gacacccgtt acagtaaatt ttccttctgt tagtgttcct gttttgtcca
     9481 gcatgatcac atctagatca ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc
     9541 cgtttttagc tgctattgat gtggagcgag caacaactaa tgggatagct aaacctagag
     9601 catgaggaca ggcgatgata aagactgtta ccatacgttc taatgcatta ggaagatctg
     9661 tcaagaacaa ccaggcgatg aatgccagga tacccacaat taatgcgaca taaaacagcc
     9721 attttgccac tttgtcagat aatgactcta gcttggattt ttctccttgt gcttttcgca
     9781 ccatttccat aaccttcgct agataaccat tttcaccagt tccagttact tcgatttcga
     9841 tcgttccatc cccattgata gaaccgccaa tcactttgtc attggcttgt tttttgattc
     9901 ctcttgattc gccagtcaca gctgattcgt caacaattgt ttcacctttc aagattacac
     9961 catctgttgg cattttatca ccagaccgga cgatcaaacg atcaccttcg tttacttctt
    10021 tcaatgaaac tgtctcttct tttccatctt tcgtcaagcg tttcactgat tctggcagta
    10081 attctgctag tttttgtaaa gcattgctgg cattcgacac agcattcatt tcaatccagt
    10141 gacctagtag cataataacg atcaaagtag ccaattccca gaagaaatcc atgacgtgtg
    10201 tatgtggatt gagcagattt gcgataaacg aatagacact ataaatatat gcaactgtaa
    10261 tccccatcgc gatcagtgtc atcatagcag gacttttttg tttcaattcc atcttagctc
    10321 cacttaaaaa tggctgaccg ccatagataa atagtatagt tgctaagatc aatacgagcc
    10381 aatcagaacc aggaaacgtt acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa
    10441 gaatgattgg gatagctagg ataagcgata gccaaaattt ttgtttgaaa ttccccatgt
    10501 gcatggaatg atccatgccg ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt
    10561 tgctgtttgc tatatctcca tggttatgtc ccatgtgcat ggaatgatcc atctgactat
    10621 ggtcatgttt catctccatc tgtttttcat gagattctgt actttctttg tttactactt
    10681 cttttttatg ctcatgtttt ttctccatat cgttcccatt catttttact ccatcccttc
    10741 tcttactttt tcatgtgtcg atttaagctc agggagttca acaatacact gacggaacta
    10801 aacgccatcg ctcctcctgc aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata
    10861 ccaatcgtat tatagacaaa agcccagaaa agattttgtt tgatttttct caatgtcaac
    10921 ctagacaact cgattgtttg tgcaatggat gccaaactgc tattcatcag cgtcacatcg
    10981 gctgtttcca tcgcaatgtc cgttccactt cccattgcca taccgacatc agctaatgct
    11041 aaggccggtg catcattgat tccatcgcct gccataccga cttttttgcc atctttctga
    11101 agtttttcta cgtaggctgc tttatcctct ggaagtactt cagcaaagat atgctttgga
    11161 tcaataccaa cttgtttacc aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata
    11221 aaaacatcta atcctttatt ctggagctgc ttgattgctt gtttggcttc tagtttgatt
    11281 tgatcagcca cggcaatcca cccgatcact tgcttttcat cagctaaaaa catgactgtt
    11341 tttccctgct gttctaattc taacgcgtgt tcttggtatt catcaaatga taggttcaat
    11401 tcaattaatc gtttacgtgt acccgcaaaa tagcgtactc cattaatcgt accgctgatc
    11461 ccagcacctg ggtgggctgt aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt
    11521 ccgtattcca cgattgcttt acctagtgga tgttcagaag cttgttctaa agtataaaaa
    11581 atagatagga cttcttttga accgactaga tctgtaactt tgggctttcc ttctgtaatc
    11641 gttccagttt tatccaaaac aatgctgtct aagtgagcag ctgcttctaa tgcttctccg
    11701 cctttaatta aaattccatt tcgtgcacca acacccgttc ctaccataat agctgttggg
    11761 gtcgctaaac ctaatgcaca tggacacgca atgactaata cagagacact atggatcaat
    11821 gcgagttgcc aatcaccagt gatcaaacct gtagctatga gcgtgataaa cgcgatgact
    11881 aatacgatcg gaacaaaaat ccctgagatc cgatctgcga ttttttggat tggggctttg
    11941 ctcccttgag catcttccac catttgaatg atttgcgcta atactgtctg cttgccgact
    12001 tgtgtgactt ccatgtgtag cagaccattc gtattgattg tcccgccaaa aagcgtgtca
    12061 tccggattct tttctacagg aagattttca ccagtaagca tactttcatc tatcgcactg
    12121 cttcctgaaa taattcgacc atctgctgga atctgctcac caggacggat gactagttga
    12181 tcaccaacga ctacttcttc aatcgctaac gtttcttctt tcccgtttct tatcacttgg
    12241 gcggtttttg tttgaagaga catcaattgt ttgattgcat tacctgtttt cgtttttgct
    12301 gtatgttcca aatatttgcc gagcaaaatc agcgtgatga tcatactgct gctttcaaaa
    12361 taaagatctt gggggtgtcc acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc
    12421 gatgttccaa ttgctaccaa tacatccata tttggtgctt tcgtcttgat tgcatggtac
    12481 gcacctttat aaaaacgagc accgacataa aattggacag gagcagacag cactaactgg
    12541 acgattggaa aatgaaaaaa gtgaacaatc gccgcatggc ttcctaacat catagcaatc
    12601 attgacaaca tcaacggcaa agtcaaaacc gtactcaata taagatcgaa tagcattttt
    12661 ttcaaatacg cttgcttttc ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt
    12721 gcaccataac cgatgttttc tacgctttgg attaattttt cagtggtcgt cccttcatat
    12781 ttgacacttg ctttttcagt ggccaagttg actgtggcat tgaccacacc aggttgtccg
    12841 ttcagctctt tttcgatcct agcagaacaa tttgcacaag tcatacctgt aatgacgaac
    12901 gtatccattt tggtttctgc tgttgccatc aaatcacctc tgctttataa cccaatccat
    12961 tgattgcttg acaaatcttt tcggattgaa cttttgtttc atcaaacttg accgtaccgt
    13021 ttgctttttt caaatttact ttcacttttt gcacgccgtc aagtgcgctt actgtttctt
    13081 ctactcttgc tgcacaatga ttgcagctca taccttcgat tgaaaattgt tgtttcattt
    13141 tttcttcctc ctattgatgt tgtttacatt cacattgacc tgggatacag ttacattcga
    13201 tcgtatctac tggttgcttt tgttccagtt gtttcaaaat ctcattgata tcttcttgag
    13261 tcaaagtggc ttcttctact agatccgcaa tcgtctctcc aatccttttt gcacagatat
    13321 gggaaaaaag attttccgtc gcactgcgaa ctgtttccat ctccgagaca gctggatgat
    13381 aaatgaattt ttttccttct tgttctgtcc agatcacttc tttttttacc aaacgtccca
    13441 gcaaagtttt gatcgttgct ggtttccaat ccatcgaatc agctaagatc tgcgtgattt
    13501 cttgggctgt cgtctgtcca agtgtccaaa taacacgcat gatctcccac tctgaatcac
    13561 tgatctttac tggcgttttg actttttcca tttatttcac acctttcgtt tacaaatgta
    13621 atcattagct tatgaataca gattacgctt gtaaacaaaa actgtcaatt atttttgctc
    13681 tctattttca atttagttaa aaattcttgt ttctctcgtc ttgttttaag gatgatcagt
    13741 ttatttcttg cattagctac ttggatcagc tgctgtattt tcggttcatt gttttcttca
    13801 aaatgccaga taaaagataa aaattctaag tattccctgt ttagtttctc tgaaaatgat
    13861 tcaggcatat ctggccgaga ttttttgttt atctttcttt ttagagaacg ccaaactact
    13921 cggtagatgg cgagataacg cggcacttta agccaaatga tcttatctgc ttctttaatc
    13981 ctttcctcta atgtagaagt ataatttcct tcaacaatcc atgacggatt agataccata
    14041 aacgattgct gttcttgcaa aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc
    14101 cataaaaggt ctagcggcag tagcggaaag tcggtgattt ctttgatctt ccttgcgagg
    14161 gtcgattttc ccgacccact ttgtccaatc acgatgatct tcattcttct ccctcacttc
    14221 tctttggaat ttggttcgat cataccactt ataacgccgc aaaaaaacaa gggactgtga
    14281 caaaagtcgt gtcaaagtcc cttgtttcga ataaatggtg ttcaatcagc tgaccttcag
    14341 cattaactca aaaaacggca aaatatgagg agctattttt tcgtttgttc ctctaatact
    14401 caggtcaaat cgctgatttt acaacctctt ccttaaacgg tgttctcctg ccaacacctc
    14461 ggtcacaagg cacaatagtc aacaaacatg agaagttgtt ttttgaaatt gctccttgtg
    14521 actcggagtt aaacggcagg ctcactacct ctttcttaaa cggtgttcaa aagcttgcat
    14581 ctgcggtaat aagcccagca aaacgaaaaa tatgaggggc tatttttgtt tggctgttct
    14641 tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc cagaagtagc
    14701 ttctgcgaaa aacaagccga aacttcacaa aaatttgaga agcaattttc gtaaattcct
    14761 tcttatttct tgaagctgac cacttctgtc acaacctctt gtaacaagta tttctatttt
    14821 aaggctgaac cactgtttct ggttcagtga tttctttgtt tttgtctcga cgagcaattg
    14881 ctggcaagat catacctaat aagatcaaga caattggtgt gatgatattc gtgaataatt
    14941 ggaaccacca tgctttagga tcagcagtat aatctacttt tggcaccatt cctaggatac
    15001 atgcaaacgc agtgaaggca aagcaccaag caccaaagat aaaaccgatt tttggatttt
    15061 taacaaattt atattcagaa ttgaatttct tataagcttt attcaacatc atatatgcca
    15121 agaacaccca taggtaacgc attggcatga caactgagtt caagttcgtc aaccatttta
    15181 ctaattcatt catatctttg ataccaaaga tcggcaacaa aataatgatt cctactagta
    15241 tacctgtcag aagataacca ttcaccaatg tacctttacg attacgttta cgtagccagt
    15301 ttggtacaaa ttctggatcc gcatctgcta acaagatttt tagtggtgca tcaattgaaa
    15361 aggctaatgc ggagatttgt ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt
    15421 taccgatacc atagtaatta cctaaaattt gaaaagcact gtatgcccca tttgccatca
    15481 agtctttagg aatattatcg ctagcaaaca gcatacccat tgcgactgag cctaatacag
    15541 cacaaatccc taccattgct gctaagaaga tcatcccttt aggaaattcc tttgcaggat
    15601 ttcttgtaga gttgacataa ggggatattt tttccgctcc tccaactgca aatactagca
    15661 tcgaaacggt tgtgaaataa ctgaaatcaa atgttggaat atatgttttc acttgattca
    15721 tatgtggtgt ggcaaattcc atcgttggat tgatgaacgg tgccgttaca gctaaaagaa
    15781 taaataagat cgacatgacg aacatcgctg ttccggccag tccgccaatc actttcaaag
    15841 tagaaagtcc ttttgttgat aaatataaaa aggcaagaaa tataataagt gaaataactg
    15901 ctaccatttg tactgacatc gtatttacga tatttccatt tccttggact gcccaaccgc
    15961 cagcaatcag gattgcttga ggcttttgtg ccaaataagg aatatggacg acccagtaag
    16021 tccaagcggc atagtatgcc agtcgtttat tagatgtcgt attttcgacc catgaactga
    16081 cgcctccttt actatccttg aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa
    16141 taaaataaat ggccaaaatc aatagccaag aggtgacaac tgatactcct tgctgagcat
    16201 agttattaac tacattcccc atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa
    16261 accagcgtaa ttgtttgttg ttcattcgtt ctctccttta tgataaaaat ttgttatcca
    16321 gatgtaatca tattcaattt ttaacaaact tacaagacag tgttatttta tgtgtgtatt
    16381 cttttattta atacacatcg ttttttcata ttttttccat caaaaaaata agttattttc
    16441 ttttatttca tttaccttat tgaatttcaa gctattttga attttctgca aaattaaaaa
    16501 cattagttat tttataatcc tatctatttt ctttacattt ttcattaaga aaaggatata
    16561 aaaaaaagag gacgttcatt cgtcctcttc attgatcaac gtttattttt tacatgcaag
    16621 cttcgcatgg cattcaaaac agctaatact gtcaccccaa catctgcaaa tactgcatct
    16681 cccattgatg caaaaccaaa agcacctaag gcaagtacca aaatttttat cccaatagca
    16741 aagacaatgt tctgtttgac gattttcaat gttttctttg ctaaatggat tgcttctgca
    16801 atcttcgatg gttgatcatt catgatgact acatctgctg cttcaattgc agcatcgctt
    16861 cccaatccac ccattgcgat tcctacatct gctcttgcta gcaccggtgc atcgttgatt
    16921 ccatctccga caaatgcggt ctttttattg tttctttgta agatttcctc taaccgctgc
    16981 actttatctt ctggaagcaa ttcgctatat atcttgtcca cgcctacttg cttcccgata
    17041 tgatcagcaa tttttttcga atcgcccgtc aacatgactg tgttcttcac accagccttt
    17101 tttaattgaa ccaatgcgtc tacggcatct tctttcaagg tatctgcaat aacaagataa
    17161 ccactgtaac tttgatcgat cgctagataa agcaatgtac cgatttcttg acttgaagga
    17221 aaagagattt caaatctttc catcaatttg tgattacctg ctaatacttg tttgccatca
    17281 acttcagcta agatcccttc acctgcaaat tcttggatat tagctgttgg taaaagcgga
    17341 cctgcgtagc cttctaaaac agattgagca atcggatgtg tagaaaactg ttctgcactc
    17401 gcaacatatt gcatgaatat ctttggatcg atttttgtat cgatcgtctg gatcgaaaag
    17461 tcacctttcg ttaatgtgcc ggttttatcg aataccactg tctcagtttg tgcaagcaac
    17521 tccaagtaat ttgaaccttt tactaataca ccgattttac ttgctcctcc gataccgcca
    17581 aagaaactaa ggggtacgga gatcactaat gcacatggac atgagatcac taaaaatgtc
    17641 aatgctcgat agatccattc agaaaacgcc gctccagaaa caacaagtgg aggtaaaaca
    17701 gctaataaaa cggccaatcc tacaacgact ggtgtgtaat agcgcgcgaa tttagtaatg
    17761 aaattttcag ctggtgcttt tttactgctg gcattttcta ctaattctaa taatttgctg
    17821 atagtagaat tttcataaga tttttctact tgaactaaaa atggtttatt tttattgata
    17881 aatccactta atactgtttc accaacatat acagatttag ggactgattc tcctgtaagt
    17941 gctgacgtat cgactaatga ttctccttcg atgatcgttc catctaacgg tacacgttca
    18001 ccaggcttca caagtacaaa ctgaccgatc tgcacgtctt cagggttcac ggtcatcaat
    18061 ccttccgtcg tctgcacgtg agctgtttct ggacggatcg ccatcaattc gcgaatcgat
    18121 tttcttgaat gactgactgc caatccttgg aaatactcgc cgatttgata aaatagcatc
    18181 actgctacgg cttctggata ttcaccgatc aacatagcgc caatcgtagc aacagacata
    18241 agaaaattct catcaaagat ttttcctttg acgatattca tcactgcctt cttcactaca
    18301 tcatatccta tcagcagata aatcaataaa taccctatca agcgaattgg ttcattaaaa
    18361 tcaaataata gcaataaagc gatacctgca agggaaaata aaatccggaa cagcgtattt
    18421 ctctcttttt catttccatg ttcagatact ttttccttat ctatttctgt tactttgaca
    18481 tctggttcta attccttcac tacttttttt atttcttgtt caatcgtagc caaatgatct
    18541 tcctctgcgt caatcgttag ttttccagaa gtgaagttta ccgttgcttc cttgactcca
    18601 ttgatcttct gaactccttt ttcgattttc attgcacagt tcgcacaatc taaaccttct
    18661 aatctgtaac ttttcatttt aatgacctcc tcgcctcaac atatgaatat ctcttcataa
    18721 ctaattataa atgaacggtt attcatatgt caaggtatcc gaaagaaaaa atgaaaaaca
    18781 tctgcatcgg aaaatttctt ccatacagat gttttggctt attctttctt tttcttttcg
    18841 atcattgcca ctgctcttat ccaacccgca cttgccttct tgatcttgac aggatagcaa
    18901 ctaagtttga agcctgttgc tggcaatttg tctaaattca ttaatttttc gataggacaa
    18961 tatctttttt caatccctgc aaaatgtcct tcccagatat tcgaagctcc actaagcttt
    19021 caaaaatata gcaagagttc tttaatgaaa attatttcaa ctttccataa acagtaatcc
    19081 catataaata aaagacgtaa aagtcacatg ggagaactta tacaggaaaa cacatagagt
    19141 caggtgaaat acatgtatcc cacctgactc tatgcgtttt ctaaccttta ttattctctt
    19201 tttatacaat ttctgcaccg aatggcatca acgccaatcg agcaagtttg aaagattgct
    19261 tggcaaaagg tatccctatg atcgtgatac atagaaggat gccgctgacg acatgtgcga
    19321 gagcaattgg taggcctgaa atgatcagcc agatgatatt aacgatcaat gatatgccag
    19381 actggtgata aattacttct ttgccaaaag gcgcaaaaga aagggaggcg atcttaaaac
    19441 attgcatacc gattgggata cctatgatcg tgatacacca aagtatcccc gctgctaacc
    19501 agctaagacc accgattagt ccgccgaata taaaccagat gacatttcct aaacaactca
    19561 ttatatttct ccttctatta ctgtttttga ttaaaactgg gcaaaaaatg attttattct
    19621 tcttttttta gcttccagca tcaaatattc tgcaaatttt ttttggattt cttccgtatt
    19681 ttcttttgtt actaatgtgt gttggattag ttttcctgat ggtcgctgat ccaatcgttc
    19741 ttctgactgt tgcacgtccg cttgcgcaaa tccgataatc cgtttttctt ttgttacagc
    19801 aacgagcaat agtttattct tatcatttag gtcagctgcc gtttttgctt ttacgaatga
    19861 ttctggatga tcagtaatca gctgattgat ttgaggtaaa tcttctttta ctgcccacct
    19921 tatttccatc atttttcact tttctttcgc tcatttttat ctccactaaa aaaatcgact
    19981 gcggcaattg catctatcca gctttgtgca atgatctcat gtccaccaat cgttgggtgg
    20041 accccatctt ctcttgtata tttcgaaaaa ccataagcaa tccctaaagc attgaatata
    20101 ccatctaaag gaaccagcaa tgcgccatat tccttagcaa gtttacgaac aatttggatt
    20161 ttagggtcta agtctttacg ccagccatga cggtcgtcgg ttataggaag tacaaacggc
    20221 tccatcaaga tgatgttgca gatttccgct gtttgaagtt catttagtaa ttttcgataa
    20281 acggattcaa attgctgagc agatttctca gttccgaata tagcctgatc gctagtatta
    20341 tgccaggtat cattgattcc gatcaaaatc gatacatagt caggttgtaa agctaaacaa
    20401 tcttcttgcc agcgtacttg cagatctgca attttgtctc ctcctatccc acggttcatg
    20461 cattcaaatt ccatcgttgg atattgggcg attagttttc cggcaatcaa aagtggataa
    20521 cctttaccta gatcagttgc ttctgctcgg tctcttcctg cgtctgtaat gctatcaccg
    20581 ataaataata tacgtttttt catttgtcaa aaactccttt ttgaataaaa cgatgatttt
    20641 cttccttata ttatcccata agccctatca agacgaaaga gaaaatatat gtataaaaaa
    20701 tgacatcagc ctagtccctt caaacaattg aatgaacgct catcaagtgt ctagtttttt
    20761 atgctatttt tttcttaacg tgccggattc cccttgattc cttcgttgtt tggtccagag
    20821 agatcgatca tacccaagct tccgtcaatc agaccactga tgacgccatc aaaacatata
    20881 tttataggct catctaagac ttctagtaaa tgtcccttta ctaatatctc ttctagattg
    20941 cttgggatag attggtcata actttatctg gtccgatgat ttcaagggtg aattgaactt
    21001 acatatggat tattgcatgc tgaaaatacg gacctactcc atcttcgaat gttactatta
    21061 gtttgttttc aggtgctaga tgatgcttta tttcaacagc agaagcatca tcaaattaga
    21121 tttccatttg aattaccttc tttgtaaatg tttgattgat tgcgatttcg ttatagaacc
    21181 ttgtgaatca aatagcaatt tcttttgctt tcttctcttg tctgctggaa acttccttta
    21241 tcccgtattt aaaaaaaaga actgcgagag ttcgcagttc ttttttagaa taaacggtgc
    21301 tctccggcca actcctcggt cacaagtcac aatagtcatc aaacatgaaa aactgttttt
    21361 tgaaattact ccttgtgact cggagctgaa cggcaggcgc acaacctctt gatataaacg
    21421 gtgttcaatc agctgaccct cgatattagc taaaaaaacg gtaaaatatg agaagctatt
    21481 tttccgtttt gtttctctaa tactcaggtc aaggcactga tttcacaacc tcttatggat
    21541 aaattcggtt caacaaacgt gggaatggaa tagcttcacg cacatggtcg ataccagcaa
    21601 tccaagtgac tgttcgttca aggcctagac caaaaccaga gtgcggaaca gaaccgtatt
    21661 tacgtagatc aagataccat gaatattctt tttcatccaa accgtgatta cggatttgtt
    21721 ctagcaagaa atcatagtct gttgcccgtt cgcttccgcc aataatttca ccgtaacctt
    21781 ctggtgcgat catatctgca cagatcacca cgtcttctct tgttggatgt ggtttcatgt
    21841 agaatggctt gatggctttt ggatagttta ggataaagac aggctgttcg aatgaattcg
    21901 caatgaacgt ttcatgaggt gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat
    21961 ttttcttcag taattcgatt gcatcatcgt aagtaatacg agggaatggc aattttgtat
    22021 aagtttttaa gacttctttg tctcgtccta gtacatctaa tgcgtattcg caatgatcta
    22081 aaacattttg tactaagaat gctacatatt gttcctgtac ttctaaactt tcttcttgat
    22141 gagtgaaagc catttctggt tcgatcatcc agaattcgat caaatgacgt cgtgtttttg
    22201 atttttcagc acggaaagtc ggaccgaatg aaaagacttt tccgaaagcc atagcagcag
    22261 cttccatgta taattggcca ctttgagata aataagcttt ttgatcaaag taatttgttt
    22321 cgaatagatc cgtagtgcct tctggtgcag aaccagtcaa gatcggcgga tcgattttga
    22381 caaaatgatg ttgattaaag aattcatacg ttgcacgaat gatttcattt ctgatctgca
    22441 taatcgcatg ttgtcttgaa gaacgcagcc ataaatgacg atgatccatc aagaagtctg
    22501 ttccgtgttc ttttggtgtg atcggataat cgtggctctc gcttaccact tccacatttg
    22561 taattccgat ttcataacca aattttgaac gactgtcttc acggatttct ccagttacta
    22621 acacggatgt ttcttggtgt agatttttag ctgtttggaa aacttcttcc ggcacttcgc
    22681 tttttacaac tacaccttgg aaaaaggctg ttccgtctct taattgtaaa aatgcgattt
    22741 ttccgcttga tcgcttattt gcgacccaag cgccgattgt tactgtttct cccacatggt
    22801 ttttcgaatc gatgatttga atttgttcca cgtatgactc tccttttata ttccttgaat
    22861 ttcttcagga ttaattttga cttttactct caataaattg cttgattctg cgtaccgctt
    22921 cttctaacgt agcgcgatca gtagcataac tcatgcggac attttctggt gccccaaagc
    22981 cttcgccagt cacaagcgct acccctgttt cttctaaaag atcttctacc cattgtgtga
    23041 cattttcata tttacacatg cgtagcgttt cttttacatt aggaaaaaga taaaaagctc
    23101 cttgcggctt ttcaagagaa acacccggca gttctgccac aagcggatat aacgtattca
    23161 ggcgttcttc aaaggcgttc cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt
    23221 caacagctgc atattgactg actgctgtcg gattactggt tgactgggaa gcaacagcga
    23281 tcattccatt gatgatctca gaattcccca ccgcataacc aatgcgccag cctgtcatcg
    23341 catatgtttt agacacgccg ttgatgatga tggtctgatt tttgattgct tctgaaatcg
    23401 ttgcaattgg agtaaactca ttgccattat aaactaaacg accataaata tcatcagcaa
    23461 ctatcaaaat atcatttttt actgcccatt caccgattgc ctgcagttca ttcttgctat
    23521 aaatcattcc agtcggattc gatggagaat tgatgattac ggcttttgtt ttttctgaac
    23581 gtgcttgttc tagctgctca acagtaactt tccagctatt ggattcttcc cctttcacaa
    23641 agacaggaag tccttctgcc agttttactt gctcaccgta actgacccag taaggcacag
    23701 gaatgatgac ttcatcttgc gggtcaagga tcgtttggaa aagggtatac aaagcaaatt
    23761 tcgctccgtc tgttacgact gtttgtgaag gttcatagac aagtccatag tttttcttca
    23821 gataatcaac gattgcctgt cgaagctctg ggattcctgc tgttggtgta taatagctag
    23881 ctttcccatt gcggattgct tcgattgctg cttcttggat gttttctggt gtcgcaaaat
    23941 ccggttctcc cacagtcaaa ctcagcacat ctctgccttt tgcttttaat gccttggcct
    24001 ttgccgcagc tgccaaagtg acagacggtt ctaatcgttt tgttcttttt gataactcca
    24061 ttccattcat tcctttacct tttaaattcc cttgattgtc ttgacttcat ctccagattc
    24121 aaagccaatc aggtaataat tcagtttccc atttgtttct tttcctacta cttcccacac
    24181 aggctgatca tcatacatcc ctaaagcagc tttttcgatt tggatttcag gatgggccgc
    24241 agcaattgtc tgttttgctt cgttttcagt cagtcccttt gtttgatcca atacttttac
    24301 tttttcacca gatttaggaa tgataacggc gatttctttt cctttatcat ttgtacctgt
    24361 cagtgaaaag tatgtcttct ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt
    24421 tgcatatttc tttgcgattt ctactgcttc tttcttcgcc tgcaccgccg gacgactgga
    24481 acgcaagtaa aaaatcgagc tgatcacgat gaccgctaat aaaaatacga tcaagccaag
    24541 taacacgcga ttaatcgttt tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt
    24601 tatcttcatt ccttttcatt atagcagatt ttaattatga aaaaagacag ctcctctaac
    24661 ttccttcttc atttttcttt aagaattcat ggatgtcctg atagatttcc tccatagcta
    24721 gctctttgat cggtactttt ttcggtaatg catggatgat ccgtgcacca tattttgcag
    24781 tgactagtct tcgatccaaa ataagcaata cgccacgatc tgtctccgtc cttatcaagc
    24841 gacctaatgc ttggcgtaac ttcaaagctg ctttaggtac ggattcctgg taaaaaggat
    24901 tgatcccctc tgattctaaa taagcgttcc tagctttgac catcgggcga agcggattat
    24961 cgaagggtaa acgtgtgact acaacgatct gcaaagcatc tccaggcaaa tctacacctt
    25021 cccaaaagct gtcagcgcca aacaatacgc ttttttctga caaaatgaag cgtttcaaaa
    25081 gcttctcccg gcttccgcca aacccttgag ctagtatttc tcgtcctttt tctagcatag
    25141 acagatgcat tttctgatag accttctgca aaacatcatg agaagtgaat aacacaagta
    25201 ttggccgatc ttcttgttcg atcagacctg taagtgctgt ctctaaataa tcgatatatg
    25261 cttgattgct gacttgatgg acggcaggtg cctcttttgg cacatagatc cttgcctgtt
    25321 tctcataatc ataaggagag gcaatcactt taagaggtgc tgcttcaatg ccaagccgct
    25381 tagagagata gtgtcgatta gggccgatct tcagcgttcc tccaatataa agaatacgat
    25441 catatcgagg ataccaaaca gtcgacggca acatagccgc ttgaaaatca tggagatgaa
    25501 aaatcatctg ctgcgctgtg ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt
    25561 cttccagcca ttgatccatc agcaagtact gcttgtctaa ttgatcaaat aagctatata
    25621 aacttgctaa atcgatccgc atctggtttg tccagctttc ttggataatc tcaaaagtca
    25681 gacgcatttg tgactgtagc tgctggatct cctgataaaa aagctgcagg tgatcaatgg
    25741 ctgtcccaga ttccagtggc agctgatcca acatctcttt tgtcatcatg acttcttctg
    25801 gatactgtcc gttaggttgc ttgccgctca ctaaactcat taatccttct gccatacaaa
    25861 tctgtgcttc gcatagcgca gaaagttctt ctcgataaat cgataaccag cgtaaggctt
    25921 catgttcttt cggtaaatag ctttgtattc ggtcaaacag ctgatttggt tcttctaatt
    25981 gctggatttg tttatggaaa tagttaatac ttaatcgttt gtcatttact ttttcagcga
    26041 tttccggcaa atgatgtact tcatcgatca acaaaaatgg actagctgga agcaatggtt
    26101 cttttcgctg tgtttcttgt attaaaaacg catgattgac aatcaaaaaa ttactttgcc
    26161 tgattttctt ttgcaagaaa cgaacaaagt cttgttcgta aaatgactga tttcttgcta
    26221 aaaatccaat acctctgtgc gcgatatctg caaagaatga atggttcaaa tttgttaaat
    26281 gtaattcatc caaatcaccg gtcttcgttt gcgtcagcca aactaagacc gccatttgat
    26341 acaaagcata ttgtttttgt tctggcggtt ccacaagtgt gcctttaaaa cgctgtaaat
    26401 caatatagtg gcgatagctt ttgataatta caggttgtat gggctgatct agcaattgat
    26461 tcagcaatgg aatgtctttt tctatgatct gctgctgcaa aactagcgaa acggtactga
    26521 tgacagcggg tttttcaggt gtagccaaat aactcaatgg cagcagataa ccgattgttt
    26581 tacccattcc tgtagctgct tcgatcatca aatcttttga ttcatctttt gtgaaatggt
    26641 cgtagaccgc attcatcagt ctgttctgtt cttttcgata catcagtgtt ttaccaaaca
    26701 ttttttcttt tgcttgtttt tttcttggat atgtccgctc accataatgg acggatgtga
    26761 atagttccac ttcttttttc tgcaaagcta gcccatcgac gatttctaat gagggatcaa
    26821 gtggctctgg tttttctttc atttcttcaa tcacatgata aatgaaacga ctcgtatcca
    26881 tacctgtgtg tctacttaat cgcgcaatct gctctaacgt gatgattggc aattttctca
    26941 tccgttcttc gatcagcaaa agaagttgac ctgtcacttc ggcatcacta tctgcctgat
    27001 gaggattctc atgaataaat ccaaggcttt ctgacaaatc agctaagcga aagcttggtt
    27061 ccgtcggcaa gaaaatttgt gcaagttcta ctgtatcgat gcctggtatc ttcaaactag
    27121 gaagtccgca tcgcatgagt tcatggttca aaaaattata atcaaaataa atattatgcg
    27181 cgacaaatat ggtgtctgcc aaaagattgt aaatcgtttg ggcgacatct tcgaaataag
    27241 gcgctttttg gacgcgttga ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct
    27301 tccgccctgg atgaatatcg atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga
    27361 cgcagccaaa ttggatgatc cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa
    27421 cgactgcata agttcttggt ttcaaaatat cttcctcttc tcctgcctct aatgttcttt
    27481 tgaaattata ctacaaagtg cagaaaaata ctctcatcgc tttttaaaga a
//
LOCUS       DAPBCS010000038        27379 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPBCS010000038 DAPBCS010000000
VERSION     DAPBCS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.038"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..262)
                     /locus_tag="RD521_001862"
     CDS             complement(<1..262)
                     /locus_tag="RD521_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771574.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLT"
     gene            complement(624..1079)
                     /locus_tag="RD521_001863"
     CDS             complement(624..1079)
                     /locus_tag="RD521_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771575.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(1233..2441)
                     /locus_tag="RD521_001864"
     CDS             complement(1233..2441)
                     /locus_tag="RD521_001864"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDT7771576.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2553..2885)
                     /locus_tag="RD521_001865"
     CDS             complement(2553..2885)
                     /locus_tag="RD521_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDT7771577.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3033..3908
                     /locus_tag="RD521_001866"
     CDS             3033..3908
                     /locus_tag="RD521_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDT7771578.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3990..4571)
                     /locus_tag="RD521_001867"
     CDS             complement(3990..4571)
                     /locus_tag="RD521_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDT7771579.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4577..5836)
                     /locus_tag="RD521_001868"
     CDS             complement(4577..5836)
                     /locus_tag="RD521_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDT7771580.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5838..6884)
                     /locus_tag="RD521_001869"
     CDS             complement(5838..6884)
                     /locus_tag="RD521_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771581.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(7119..7394)
                     /locus_tag="RD521_001870"
     CDS             complement(7119..7394)
                     /locus_tag="RD521_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDT7771582.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7637..8947)
                     /gene="der"
                     /locus_tag="RD521_001871"
     CDS             complement(7637..8947)
                     /gene="der"
                     /locus_tag="RD521_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDT7771583.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(9129..10358)
                     /gene="rpsA"
                     /locus_tag="RD521_001872"
     CDS             complement(9129..10358)
                     /gene="rpsA"
                     /locus_tag="RD521_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDT7771584.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(10493..11173)
                     /locus_tag="RD521_001873"
     CDS             complement(10493..11173)
                     /locus_tag="RD521_001873"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDT7771585.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(11222..11857)
                     /locus_tag="RD521_001874"
     CDS             complement(11222..11857)
                     /locus_tag="RD521_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7771586.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11923..13326)
                     /locus_tag="RD521_001875"
     CDS             complement(11923..13326)
                     /locus_tag="RD521_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDT7771587.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(13313..14344)
                     /locus_tag="RD521_001876"
     CDS             complement(13313..14344)
                     /locus_tag="RD521_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771588.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            14375..14596
                     /locus_tag="RD521_001877"
     CDS             14375..14596
                     /locus_tag="RD521_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDT7771589.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14761..15498)
                     /locus_tag="RD521_001878"
     CDS             complement(14761..15498)
                     /locus_tag="RD521_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDT7771590.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15651..16991)
                     /locus_tag="RD521_001879"
     CDS             complement(15651..16991)
                     /locus_tag="RD521_001879"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDT7771591.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(17228..18751)
                     /locus_tag="RD521_001880"
     CDS             complement(17228..18751)
                     /locus_tag="RD521_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDT7771592.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(19129..19740)
                     /locus_tag="RD521_001881"
     CDS             complement(19129..19740)
                     /locus_tag="RD521_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7771593.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19863..19988)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(20070..20786)
                     /locus_tag="RD521_001882"
     CDS             complement(20070..20786)
                     /locus_tag="RD521_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDT7771594.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20792..21361)
                     /gene="scpB"
                     /locus_tag="RD521_001883"
     CDS             complement(20792..21361)
                     /gene="scpB"
                     /locus_tag="RD521_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDT7771595.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(21345..22142)
                     /locus_tag="RD521_001884"
     CDS             complement(21345..22142)
                     /locus_tag="RD521_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDT7771596.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(22112..22483)
                     /locus_tag="RD521_001885"
     CDS             complement(22112..22483)
                     /locus_tag="RD521_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDT7771597.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(22502..23389)
                     /gene="xerD"
                     /locus_tag="RD521_001886"
     CDS             complement(22502..23389)
                     /gene="xerD"
                     /locus_tag="RD521_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDT7771598.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(23485..23958)
                     /locus_tag="RD521_001887"
     CDS             complement(23485..23958)
                     /locus_tag="RD521_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7771599.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(24063..24926)
                     /locus_tag="RD521_001888"
     CDS             complement(24063..24926)
                     /locus_tag="RD521_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDT7771600.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(25122..27221)
                     /locus_tag="RD521_001889"
     CDS             complement(25122..27221)
                     /locus_tag="RD521_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDT7771601.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         7505 a         5803 c         4613 g         9458 t
ORIGIN      
        1 gtgtaagcat ttttaaaaat tgttcttttt tctttaaaat tttggtttcg actacttcaa
       61 cctgtgtttg gttcccctct aattcacact gtacacgttt tgactccaaa cgaagcagat
      121 catgtttcag acgttccaac tgacgataca tcaatatatt tcttttacgc aacttacaaa
      181 gtatttgacg gcggtcatcc atcgaaaggg cgccatagcg atcatgcaac agtttttttt
      241 gttgttcaaa actctctctc attttccaga tctcctatac acaaacttcc atcattatag
      301 caaagttctt tttggatgaa aaatatttca ttgtttcatc gtttattgct agttagaaat
      361 ttccataaat ttttcaacgc ctcattctat tccaaatagt atcagatatc aagtaaaact
      421 taagaaagat aaaagtttct gacaaaagga tgtttacttt cccattcctg cctttcttgt
      481 tcttttatca caacaaaaaa atggggctgc gacaaaaatc ttgtcacagt cccttattct
      541 gaataaacgg tgtggaagag acagcctatc agtagtaata agacacatct ctttcacagc
      601 ctcatttttg cgtaaaaatc gatttattcg aaaaattccc cgtatacatc ttctacacgt
      661 tgatctggat tgatcacgat atacaagtta cctgtttttt cgctgatttt tgttgattga
      721 tattgattgt ctaatccttc tgttccttta tctagatatg ggaagtacac aagatcaggt
      781 gtgccatttc ggtaaaggat ttccattcgc tcgatatctt cgtatttcaa tgcgcgatta
      841 aacatgccca tttccagtcc gcctaggttg atatcatgtg tcgcaactgc gtccccttca
      901 tggaagattt cgattttaaa tccagcacac ggatggattt caacaaattc actgccattg
      961 attcgtccaa aacttgttgt gatttgtttg atccaaagat cgccgatatg acggcggttg
     1021 atcgtccagg tttctccatt tctaaaataa aattttagcg ctgtcatttc tttactcatt
     1081 aaaaagactc ctttatgatg taatttattc ttgtactacc tcagtttagc acaaatttat
     1141 gtaagtggtc acatcttatg ttttttcttc taatgacgta tctgcccact ttaagtagcc
     1201 aacaaagctg agcagataca gtctagtgtt ttttaaatca atttttcttt cgataaaaaa
     1261 tccaaaacct gttttttatc attttttatt ctttgttcta cgacggcttt ttcagctaat
     1321 tgcaaagctt cactaaccca cttaccaggt ttttttccag tttcttctag caagatttta
     1381 ccatctactg ctaattcgct taatgacttg ataggtaaac gatcatagga ttctcctacg
     1441 gcatccatct gaggtgtttc gtcaaaatag atcatcaatt cttctacttg cagtgcatgt
     1501 tttttgccta agaagtacaa ttcgaatggt tccaaagctc cagtttctcg ttgtcgtagt
     1561 ccttggacaa gtgcttgaac gtctttaatc atttggttag aacacttcca cgatttcaaa
     1621 aaggaacgaa tctcattttc ttttagatcc agttggctga tcaataatgc ccatgcttgg
     1681 ctctcttctt tcaatgggac ggatggtaat tcagcaaaac gtaaaagtgc ttcgccttct
     1741 tctttcaaac cgggacagca aatgtagcac tctgtttcaa taaatgcatg gattcccgcg
     1801 gcccgttcat ttcccaagag catcttgatg aattccacat ttatacgttc tacagatatt
     1861 ttacctaata gctcgtggtt ttctttgatg gcgtagaagg tttcttcctc taatttgaac
     1921 cctagttgac tgacaaaacg caacccgcgc atcattcgta aggcatcttc atggaaccgc
     1981 tcatgaggat tgcctacagc tcgcaagatc tgttcttcta gatcttgcag accatcgaac
     2041 aaatcgatga tttctccatc ttctttcaaa gcaaacgcat tgatcgtaaa atctcttctt
     2101 ttcaaatctt ctgccaacga acgaacgaat tcgacatgat ctggtctacg gtagtcctga
     2161 taagttgatt ccgtccgaaa tgtggtgatc tcatattgtt catcattttc taataccaac
     2221 acagttccat gttcgatccc aatatctatc gtttttggaa aaagctgttt gatttcttct
     2281 ggaaatgcgc ttgttgcgat atccacatca tggattggct ggttcagcaa gatatcacga
     2341 acacttccac ctacaaaata agcttcatat cctgcattct ctaatttttt cagaactgga
     2401 attgcctttt ggtattccag aggtaattgt tttaatttca tttttccaac ctttcctgcg
     2461 tgtttcttgt cctgagataa agactcagtt gcatggtgac tgtttgacaa gtccgtttca
     2521 gagtcttcaa ggccaggacc acttcatctt gtttactcgt ccttacgctc aaagcgatta
     2581 tggtctcttt gattgaattt ttccatgttt ttttcaaaaa cttcagttaa gtcgatattc
     2641 aatgaattag ccatgatcat cgtaacgaat aaaacatcac caagttcttc tgctaccgag
     2701 ttaactggtt cagaagcctt tttgcttttt tctccataag tgtggttgac ttctcttgcc
     2761 aattcaccta cttcttcagt taatcgagcc atctgagcta atggagagaa ataccccacc
     2821 ttgaattgct ggatatattc atccacttcc tgctgcatac tgtataaaga acgcttttta
     2881 tccatctttt tccctccaaa actatcttac caaaacttta acgcaaatcc tagataagag
     2941 caattgagaa aatcgaattt ccatgctaca tttaggaagg aatttctaaa tggaaattct
     3001 gtgaaaatac ataattgaaa gctggaattc atatggtcaa acgaaaaaca gtaaaagaag
     3061 ttctttttat cattatagga acaagtattt atgcatttgg tcttgtttac ttgaacatcg
     3121 ctaatcattt agcagaaggg ggagtctcag ggatcacctt gatcttaaga gctttatttg
     3181 gtattgatcc agcttatacg actttattaa tcaacatccc attgatttta ctaggaggaa
     3241 agatcctcgg taaacgttcc ttggcctata ctgttctagg tacactttct ttgtcattct
     3301 ttttatggat ctggcaaagg gttccactag aaatcaattt acagcatgat ctactgatcg
     3361 tttctctact tgcgggttta gttgcaggtg taggaagcgg gatcgtctac cgcatgggtg
     3421 gaacaacagg cggcagtgat attatcgctc ggattttaga acaaaattac ggcatcacta
     3481 tggggcgttc cctattggca tttgatatca tcgtcttact tgcttcatta acctatatcg
     3541 acttgaaacg tatgatgtat acgttgattg tttcgtatgt tttcagtcgt gtgattgatt
     3601 cgatcttaga tggcggatat tctgcaaaag gtctacttgt cgtctccaac aaaagcgaag
     3661 agattgcacc actactgatg accggtctgc aaagaggtgt gactttcctt tctggagaag
     3721 gcgcattctc tggtaattca aaaaaaatca tctatatggt cgttagctct agtgaattaa
     3781 ctgaagctaa gaggatcgtc cacgagattg acgaaaaagc tttcctttca atcatcaatg
     3841 tgcatgaagt ggaagggcaa ggctttactt atttgaagcc acagacaaga ttactgaaaa
     3901 aactataaga aaaaagacaa agataggcta tgaggaaaga ttccacatcc agctgacctt
     3961 tgtctttttt agtactatac tatttttaac taattgttat gttcacttac ccggttactt
     4021 ttcttttgtg tctgcaaaag gatctttcgg ttaacttcat cagataattt caaaaaagca
     4081 tccctatctc catcttctag tgcttgatcg atttgagcat aaagcatttt tagttttgcc
     4141 tcttcttctt tttcttgaaa atatcgttca attaattcag tatcttcttc actgactgaa
     4201 gaatcccaag aagaaaatgg attgtcttct aagatagata gatattctct tgtctgccaa
     4261 gcattttcga atatacattc cacatataaa ggctttttcc aattcaaacg gatttcatga
     4321 aaaatctggt ctgtgtccgt aaattctttt ccctgcaaaa acaatttcat gggttcccct
     4381 tcaacagata gatcactgat ctgcagtcct cgatccgcaa aattcgcccc ttcaacaaag
     4441 tggacattgc ttaaaatcgc ttcatgattg gcaagataat tcaagatcca aaaaacttct
     4501 cttcggctaa aagatacatg attgaccatc cacgttaaaa aattcttctt ttcccgtaca
     4561 tcaattagca tttttctcac ctctctgtta ggtcatctaa taatgacagt gcctccatat
     4621 ctcctggttc aagtgttaaa taataagtca ataattcttg cgctcttttc agctgccctt
     4681 cttctcttaa gaaaaaggca tactctttca agaaatctgg ttcatgtgat agttcatgat
     4741 aagcctgttc atagtgtaca gctgcaacgg taaagtcttc taattcatta taagcatggg
     4801 ctaggttcca ttctgcataa ggattagcag tttcttccat ctgattgatc acttgtatca
     4861 catcttcata tctttcatca tccaagtaaa gattacttaa agtaagtaat gtttcatctt
     4921 gtttgtctcc tacttctaaa gctttcagta aaagttcttc tgctcgctgt ctatcatgca
     4981 gacggaaagc attttccgag gccaggtgat aaagttcgac ttggtaagga ttttctttga
     5041 ttccttcttc taacactgtt tgtgcttctt caatcatttc ttcttcttga agagcttgtc
     5101 ctagatatag atataacgat tgataatgcg gattaacttc tcgaagttct tccagatagt
     5161 tgattgcctg ctgattttct ttcaattgaa gattcgtaaa agctagctgg aacagtcgat
     5221 catctgtcct ttcttctttc agtgcttctt ctaaaggtac gatagctgct tcgaattctc
     5281 cttgcatact taaggattgt cctaaacgtt cctgcatcga gattcctgaa atttcattta
     5341 tcccgcttgc caacaatgtt tcataaacca ctgctgcctc tttgaaacga tcaactgaaa
     5401 aatacaattc agctagcgca aactggatca atggttcatc aggaagcaac ccagctgctt
     5461 ctttcaattt tgcttcactc acttcaggga ttccgataac ttggtacaaa tcagctatcg
     5521 ccaataagct ttgcggatag taatcacttt gcttatccac tttttccagg tattcaaaag
     5581 ctaagtcgat ctcattatct tcgattgcaa tttctgctaa cgggatgttc agtccgtcat
     5641 tttctgggta ttggttgatc agttgttcaa aaatctgttt tgcttcgatc aaaaagccaa
     5701 tagccagcaa ttcttcgcct agttcagcta atatatctgc tgggtcttct tttaatgctt
     5761 tttctaaaaa gatttgtgct tgtgacaagt cttcatctga taaagcttgc aacatttttt
     5821 cgctatttgt attcatttca attccctctt atctcactga cgatttgttg aaataccgtc
     5881 attgttctca ataattcttt ttcgtttgga tgttggtatc cgtctattat acctattttt
     5941 tctaaaacaa gtattttttt gcttttttct gccaaaaaaa gaactttttc caaatactcc
     6001 atctttgaaa tctgttccag ccagtggatt ggatagccaa gcaagtccag ccacacaaaa
     6061 aagtttttta gttggaaatc aaaaggatat gcttggagat tccacacgaa atgaaacatc
     6121 attcctaaaa attttttcat actagcagaa agtagatggc cattttcggt cagataaaaa
     6181 gcttgttcaa acagtttacc ataatttgac aggttttctg cctctaattg ataaaaatag
     6241 atcatttctt cgatcatccc ggcaaacggc cgagtcgtta attgcttttt atttggatag
     6301 tttttataca gattttgcaa aaaacgatga tcacaaatca ttccgcaaca gataaaagtc
     6361 aaaaagtcaa tctgctttcc gtccacctgc cgctcgctga ttgtttgatc ataaacgatg
     6421 cgttctggca gattgatggt ttgcagcact acacgatttg gtagctgcaa aatcgtccgc
     6481 tgcggtgtta atgctcggct tagagaacgg atcgatacag ggatcatcca tagtgatgaa
     6541 tcaagggttg tatgtttttg aaaaaaacct gccagatcca tgatcccttc atttccaaat
     6601 ccgatgataa acatggattg attttcggga tagcgtttga cgaaatcaag caggttcttt
     6661 aattctgtta aatgattgca ttgagtgttg gcacaaatat accagtcatt gtttgcttga
     6721 tttgcaaata acgggttgat cttatctgca aacaaatcat aatagcgctg attcgttaaa
     6781 aataagattg gctgatcaat ggaaagttct cttagctgac tgactagcgt ttctccgtaa
     6841 aacactttcg tcaacgcatc ttctacttga taatcaagtt ccatacgcat ccctcctctt
     6901 cttattttta ttttaccatt agtaagcaaa ttctagaaaa gataagagca aagctcacct
     6961 cataacttgc aaataaataa aaattaaata aaatacatgt gttttctatg tatgacatac
     7021 ggattcggaa tctctgtctc ttatttattc cttttctgct aggaaataaa aagaaaagca
     7081 cttgtagccc tagagccaca agcgcttttt caactgtctt atttaacagc gtcttttaat
     7141 gctttacctg gtttgaatgc aggtactttg ctagctggaa tttcgatttc ttcaccagtt
     7201 tgtgggttac gacctttgcg agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt
     7261 aattgaactt tttcgccgtt agctaaagca tcttgaatag ttgaaaatac agcatctact
     7321 gctgcagttg catctttttt agttaaatca gtagctgaag caactttttc gattaattct
     7381 gctttgtttg ccatggatag attcacctcc tccacaagag atatgataga aatatcattt
     7441 agttaaaact ttctgagtgg tccagaagtt taaaaagaaa tattgattaa cttcaatatt
     7501 ctgttatcac gatatcatat gaaccgctta atagcaaggt ttttaccaat tttcacatgc
     7561 tttttttagt catagaaacg taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt
     7621 cgatatggta aaatgcctat tttctcctac gagggataat tcggatggac gtcccttcaa
     7681 aagtaaatgc ttttctgatt tgattttcta agaaacgttc ataagaaaaa tgcatcaatt
     7741 cctcttcatt gacaaaaacg acaaatgtag gtggtttgac agcgacttgt gtagcataaa
     7801 aaatcttcag gcgttttcct ttatctgttg gtgttggatt gattgctaca gcatccataa
     7861 tgatatcatt caacacagcc gaagagatac gaagattctg gttcatactt acttgttcga
     7921 tcatttcagg cagacgttcc aaccgttgtt tagtcactgc agaaacaaaa acgattggag
     7981 cgtaatcaag ataccggaat tcatcacgaa tctcttgttc gaaatcacgc attgtattgg
     8041 tttctttctt tacaagatcc catttattca cgacgataat aattccacga ccagcttcat
     8101 gggcgtaacc cgctactcgc ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca
     8161 tcaaaacgat atcagaacgg tcaatggcac gcattgcacg catcacgctg tatttttcag
     8221 tattttcgta tacttttccg cgtttacgca taccagctgt atctatcatt aagaatttct
     8281 gaccactttc agaaacgaag tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg
     8341 aaacaattac acgttcttct cctaaaatcg cattgatcaa agaggattta ccaacatttg
     8401 ggcgaccaat caaactaaac ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga
     8461 aatacttcac tgcttcatct aaaatatcac caatccctag tccatggctt cctgaaacag
     8521 gataaggatc gcctagccct aaggaataaa attcatagat atcgtttctc atttctggat
     8581 tatctacttt attgactgct aaaatcactg gtttattact tctgtataaa attttcgcaa
     8641 ccatctcatc tgcgtctgtc acgccttcgc gtccgctgac tacacaaatg attacatctg
     8701 cttcttcaat cgcgatttct gcttgatgct tgatctgatc cataaaaggt tcatcgccaa
     8761 gatcaattcc gcctgtgtcg atcacgctga attctcgacc taaccattca ccttttgcat
     8821 aaatacgatc acgagttact ccaggagtat cttcgacaat cgagatacgc tcaccagcga
     8881 tgcggttaaa aatcgtagac ttaccgacgt tcgggcggcc aacgattgca attgttggat
     8941 ttgccatatt atttcctcct cacaagtttc caatctcact tagtttaacg aatgcattgc
     9001 caactttcaa gcatagattg tcaattaaac gaagtatttt tattccttat tgaaatacta
     9061 atcgcttttc aaaagaaaaa ctgagacaaa agcagctgct ttcatctcag tcacagatac
     9121 aaaatcaatt atttttcaga atcatcggtt gctttttctt ctacttcttc tttcaacgca
     9181 tcacctaaaa tgtctcccat agtaaatcca gtattttctt ctggtaattc ataagactca
     9241 acattttttg gttcttcttc tgaagctggt ttttcttcta aagctttgat actcaatgca
     9301 atacgatgtt cttctggatg gatctctaat actttcactt tcacttcgtc accttcatga
     9361 agcacttcat gtggtgtcgc aatatgttta tgtgaaattt gagagatatg gactagccct
     9421 tctactccag ggaacacttc aacaaaggca ccgaagcttg tcaaacgttt aactgttccg
     9481 tctaatactg tgccaactgg agcttttgta tcgatttctg accaaggtcc aggctgtgtg
     9541 tctttgattg acaaggaaac acgttcttgt tcaggattga tagacaacac tttgacattt
     9601 acatcatcat tgacagccaa tacatcactt ggttttgcta catgactatg tgaaatctct
     9661 gagacatgga ctagaccgtc aattccgcct aggtcgacaa atgcgccaaa atcagtaaga
     9721 cgtgcaacac gtccactgag aacatctccc tcatggatag ttgaaagaat ttcttttttc
     9781 ttttcttctc tttcgttttc cactaccgct ttatgcgata agatcaaacg attttctgat
     9841 ggttcgattt cgatgatttt aaagctcaat gttttgcctt tatagtctga aaagtctgca
     9901 acaaaatgat cttccaccat tgatgcaggg acaaaaccac gtacaccgac gtcaacgact
     9961 aaaccgcctt tgacaacatt tgtaacaggt gcttcgatga ccttgccctc ttggaaatct
    10021 ctttcgatat cttcccaaac ttttttagca tctaaacggc gttttgaaag tagatagctg
    10081 ccgttttctt tatctttgcc gatcgtactg ataacgacta aatctaatac atcaccgact
    10141 tttactactt cgctgatatc ttctaccggc aaagttgata attcttttgc cggaacgact
    10201 ccttcaactc cagccccttc gattccgaca atcacttgtt tgtcttcaat gactaaaact
    10261 tcgcccttaa caacatcgcc aacttttact tcttgtacgc ttttcattgc gtcttccatt
    10321 gactggttgt tctcttgatt ttgatcaaat tctgtcataa cacttgtcct cctaaccaac
    10381 attctgccgt aagtacggta ctgtttttag tttaattgat tattcaaaaa aaataaagcg
    10441 atttcgttcg ttttccttga aaaaacaatt gtttttcatt ttttttctta atttaaaaaa
    10501 gaagataacc ttttttcaaa acaacagctt ttattgctgc aacgacttct ggaatcgatt
    10561 tgccagtcgt atcgatacga acagcatcct ctgcttgctt cagcggagag acgtcccttg
    10621 ttgaatcata atggtctctt tcctgtatct ctttcgtcag tgtttcaaga tcagtcggaa
    10681 ttcctttttc ttggttctct ttgaaacgtc tttctgcacg ctcacttacg cttgctacta
    10741 aaaagatttt cacttctgca ttcggtaata cagctgtccc gatgtctctt ccgtccatga
    10801 caacgccacc gcgttcccct atttttcgtt ggacagcaac taattcttct ctgacttttc
    10861 catgtgcaga gacttcagaa acagcttttg tgacatctgg ttgacggatt tctaaagtca
    10921 catcttttga tgctatgaat acatgctgtc catcttcttg ttgcgaaaaa gagataggat
    10981 actttttgat cagcgaaact agcccttctt catctgaaaa agatacattg ttcgtaatag
    11041 ctaaataagt tactgcgcga tacatggcac cggtatctgt ataaatataa ttcaattctt
    11101 ttgctaatat cttggcaacg gtacttttcc cagatgaagc aggcccgtcg attgcaatac
    11161 tgatttgtgt catgatataa ctcctttttc tcagaaaaaa agccgcaaag cgcggctatt
    11221 attatttaat tcttaattct tgaccagggt aaagcataaa gttgtttgga tctaagccat
    11281 tcaattcaaa caattgatca gtggtgatcc ctgcacgctc agccacttgt tttggacctt
    11341 cgcctgcttg aacagtcgtt gttgaaccgg ctgcagctga tgaagaaggt gttgtttctg
    11401 ctgcattgct gcttgaatcc cctgcttgat aagttgcctc acttgaggaa gtcggtgttg
    11461 tctctgctgc aatactgctt tctgacgtta cacctgtatc ttgagacaca gtagattctt
    11521 ccgcgattgt actagattct actgttgaag tctcagttga ctcctctttt gttgtacttg
    11581 tagaactttc tactactgat gtcgaagttt cagaagaagt tgctgctgtt gcattgttgc
    11641 ttgatccatt tctgatccag aaataagcac ctgctggaat agcgatacat aagaccagta
    11701 aaatcaaaaa tagcgaaaga aataatgtat ttcctttctt ttgttgtctc tgagaacttc
    11761 ttgatgaaac ttcatcatct gtatcgtaga ttggttgttc ccatggttct tgtgtttcgt
    11821 tattttctgt agattgatgt ctatcttttc tgctcactgt tttacctcct aaactctatc
    11881 gtgtgtatta taccatagga aaaagtgtct tgtcctaaaa aatcaatgtt ccatgttttt
    11941 tttaaataaa tttagtaaaa tcgtttccca atgaagttgt tcagtcttag aaaaaagctt
    12001 cctctttggc agaagttctg cttttgctcc atcaatatca caacaattct gcggcttttc
    12061 ggagagtttt tcgccaaaat aagctaagat gaattttctg cggcagtttt cttcattaat
    12121 atagcgaagc atctgctgca ggcggaattc tttttctttt tcctgacgtt tgagccgttc
    12181 cacccaattt tctggttttt cagactgctg gatcagctcc atccattttt tttgcagctc
    12241 atcaaaggga gcttgctctg tggcatattc caaataaagc tcaaaacttt gtcgctgttc
    12301 tctagaaagt tgattaaaga aataatggat acgttcatcc ccactttgat aaaaaagaat
    12361 tgctgcactt tcttcctgat ctctgcctgc tcgaccgatt tcttgtacat agttttctag
    12421 actgtctggc aaatcataat ggaccacata acggatatca gatttatcga tccccatacc
    12481 aaatgcattc gtagcaatga gaaactgcag ctgatttttt acaaactgct gttgaagttg
    12541 tcttctttga gctgcatcaa gaccaccgtg atagtatcca actgtaaaac gttccctgaa
    12601 taaatgatac aatcgttcga cttctttttt tgttgcacaa taaatgattg ccgctccttg
    12661 tgctctttcc atgaagtgtt caagttcttg ttctttttgc tgcgttttcc gaacaaaaag
    12721 cgagatattt tgccgattga ctgattgtct gatctctttt ggtggtcgat caaatagtac
    12781 atgacgtata tctcttgcta ctaaatctgt tgctgtcgcc gttagtgcca gtgtgaccgg
    12841 gtttcctaaa agctttcgga tttttcctaa ctgttgatat tctggacgga aatcaactcc
    12901 ccattgagac acgcaatgag cctcatctac tacatataac gcaatatttt gttttttcag
    12961 atgctctaaa agagaaggat tagtcaacat ttcgggactc aaaaacaaaa atttatattg
    13021 cgatagatga cggaggacgt atcttctttc agattgcgaa agtaaactat tgaaagcagc
    13081 tactcgtttc tcccctcgtt tttgtaattg cgtcacttga tcttccatta gagaaagcag
    13141 cggtgacacg atgatgacca gaccttccat taaatacccc gtcaattgat agcaaaggct
    13201 ttttcccgtt cctgtaggca aaatagcgag agtatcttct ccatcaagca acgcctgaat
    13261 aatttcttct tggccgggac gaaatgaatc aaatccaaaa tattttttta attcatttat
    13321 taacattctg tatcgcttcc tttctcaata gatagatttg atacaaacga aattctccat
    13381 aatctaattg tccatctaca ttccattcgc taaagcgcca ctctcgcacg gaatccttat
    13441 ttggttccaa acgttcgatt gtttctgcag agagaatcca ctcgtatgga aaatcatcaa
    13501 aaaacaaggc ccactcaata agatgatctg ttactgttcc tctttttaaa tgacggattg
    13561 ccatgatctg gtcaattgtc tgaccattca taaacatctg cttggtgatc atcatgcttt
    13621 gattgaaatt ttgctgtaat aatgaatcga tcaaagcgtg ccaaagcgaa tctggccgtt
    13681 tttcgatttg tgctaacaaa aggtggatac atctgctttg ttgcagataa accgctgact
    13741 catcattgtt ttttgccagc tgataaggaa gcagccctgt tcgctcattt ccagataatt
    13801 ggttagctaa aaaatctgct gcttcttctg gtatctccga aaacagctgc accaaactct
    13861 catacgcagt atcaatcaaa atttcacgtg gcaattggct ttcactaagc cattttttta
    13921 gttggaaagt ataaaaagga cttgtctctg ctggaagata gtcttgaata cccgttgcca
    13981 gattagaaat tacttgaaca gcaaatttaa tgagccgcca actagtttcc cctgttctgc
    14041 catagcggtc aaaacgcaaa ccagtatgtt ctatttgatg ttcggacaac atatcagccc
    14101 ctgcaggcgt tagctttgct tcattttcat ttatttcgat ccaaccttgc tgcgcaattt
    14161 tttgcaaaat ttgataaaac ttatcctgct tcaattctgg aaaactgtta tggataaaca
    14221 aaagttcgtg ggtaaatcca taaataagta ctgaagtcgt tcgttttccc acaaggagtt
    14281 ggtaaagaga actaatccgc agcttgtgtc ctgatgaaaa tagcgataaa ataaaaacaa
    14341 tcatattatt ctccttaagt taggatggtt agaaatgtca ctactatgta aacttgtacc
    14401 tgaacgctgt attgcatgcg gtctttgtca aatcgaagcg ccagatattt tcgattatga
    14461 tgaagaagga atcgttttat ttgcccaaga accagaggct catcaacagt tcgtgatgcc
    14521 ggaagaagaa acatatgtaa aaaaagcgta tcaaaaatgt ccagttcgag cgattttatt
    14581 agaaaataaa atataaatat aaacgaaact ttaaaaacgt acactaagtt ctctaaactt
    14641 gaaaataagc cgggatctgt ttctcttgtt ccgtgaaatc ccgacttatt ttgaaagatt
    14701 catttgctat ctatagttaa ttgactagtt ttttcacgga ccctatagga cccatttata
    14761 ttatttcaac tttttcagcc agttttccaa tgtattgacg atcacagttt gctggtctgc
    14821 gttagaaatt tctgcctctc catctccttt ttgctcacca tagctgccaa aacctccatg
    14881 atttccgcca ttgattgata catactctgt tctttccggt aaatactgct tagcttcttg
    14941 ataagcttcc tgattcaata ctttatcttt tgtcgccgtg atcgataaaa caggaacagc
    15001 tgattctgtc aatgttcctt ttttgtcggg ataacttgcc atgaagaata ccccttttaa
    15061 atgtttgtct gaatgattct tattgacata acgacttgcc attacgccac ctaatgaatg
    15121 gccaccgatc acataatcgg aatttggaaa atcttctttg accttatccg ctttgtttcc
    15181 gcccatcact gctagatcaa gtggcatttt gacaatagcg actgaaaagc cgttttttgc
    15241 taaatcttgc gcccaaatac tataactttc tggctctacg agagcaccag gataaaaaat
    15301 aagtaatgga gactttccat gattcttttt ctctccttca aaaaataagt aatcttcttt
    15361 gttttttcct tcttttacca acacttgcgc ttctgacgat ggttcgtacg tattttgctg
    15421 tatatagtaa taaccggctc caccgagtaa tcctaaaatg atgactaagc caattagtat
    15481 tcgtctaaat attttcactt tctcccacct tttctatctg agtgacccct ttttcttaat
    15541 caagtatgat tataccttaa aaaaataaga gagaaatgca aactttccag catcaaataa
    15601 aaaaagacgg aatatccatt tgaatatccg ccctttttgt atgtttattt ttatttagca
    15661 cataaacgat acacagcttc tgcataaatt tccattgctt tatacatact acttaattcc
    15721 catcgttcat tcgcctggtg cataaagtca ggagtatcag ggaacatcgc accgaatgcc
    15781 acacactggt tcatcgtacg agcaaaagta gcgccgcctg atacaaacgg ttcagtcatg
    15841 tctccagtaa tatcgcgata tgtccccaat agatttttca ctaactcact gtctagtggc
    15901 acgtataatg aatcaaggaa gtcaaattct tcataagtca acccatattt ttctgctgtt
    15961 ttagttaatt tagctactaa atcatctttc ttgaatgtaa cagggattct catatctaca
    16021 ccgatttttg tttgttctgg tgtgatttcc aaacttgcaa agttcatcgt tagctcgccg
    16081 gattgttcat cttctgtttt gcccacgaca gcttcaccag ttgcattttc ttgaactaat
    16141 tttcctaaga aattgattgg gccaaaatca aatacttctg aaagagcaat tgccagacga
    16201 gaaatagcat tcactccttg tgctgcatct ttcgcgtgga tacttttacc aagaacgacg
    16261 atgccttcac cttgttcttc gaaatcgaat tcgtgttttt tcaatgcttc ttttacttca
    16321 gaaagctttt ctccagagta aggagctgca tcaggaacaa cattcaacgc tcctccagct
    16381 tttacagaaa actcgttcgt tcctggtcct gtcaagtaag cttgcaataa gcctttttct
    16441 gcataaatta aaggaaattc agcatctggc gcaaatcctt gcgtaattcc ttcttctttt
    16501 tcgttatatt tttctaaaca acgccataaa ttttcttcat ctgtaccaaa gataaaacga
    16561 atacgtgtct tgaattccac gccagcatca atcaaagctt tgacggcata cattgccgca
    16621 atagaaggac ctttgtcatc ttgggatccg cgaccaacta accagccgtc ttttatagtt
    16681 ggatcgaatg gttttgtttc ccaattattt tcgtcccctg ctggaacaac gtccatatga
    16741 cacaagatac cgaataattc atctccagag ccaatctctg aataaccgta atagccttct
    16801 ggatctttaa atgttctaaa tcctagcttt tcactgattt ccaatacttt atccaaagct
    16861 tcaatcactt ttttaccgaa gggatgtcct tgtcctgaat ctgcttcatc tagtacggaa
    16921 ggaattcgga tcaattcgct caatgattct aatgcttgtt catgatgttg ttctgtcaca
    16981 aattttttca tgatattatc ctcctaatat gttaaaaagg gtcatgtcaa attcctccga
    17041 cacaacctct tcttctcaaa taatgacgtt ttaagcctgc tccatcagca agcagaatgt
    17101 tcttaacaaa tgatgaaaga aaaaacactc actgaatgga gctattgact tatccacgtg
    17161 cttatcgata gccgtttatc ggtttcttca ttcttatgat agaccttgta cctgtatttg
    17221 tctaggttta gaacattgct gctacaccta ggatcacaca gatggctaca aataatatcg
    17281 caattaattt accggtaaat ttccaccaca ctgaaacatc gaaacgagca attgctacag
    17341 cacccattac gatagcagat gttggtgtaa ttaagtttac taaaccagat gcagattgat
    17401 aagcagtgat tactaaatcc ttaccgacgc cggcaaattc acccataggt cccatgattc
    17461 ccattgttgc agcagctaag ccggaagttg aaggaatcaa gaatgacata gggatataga
    17521 aaataaatgt aataatgatg aatacacttg aagataatga acttaatcct tgttcgcccc
    17581 aatgtaagat ggtgtctgtg attaatccat tgttcataac cacttggata ccacgagcaa
    17641 cagctactac gattgctaca ccgatcaaat cagaagcacc agctaaaaat tcagagacaa
    17701 attcagactc tttcatgcca taagtgattg ctactacgat ggacattact aagaataaca
    17761 tagtaatttc tgggaagtac cagtcaccta atggaagcat gcttttacct agaaccgtac
    17821 ctagtaccgg aaggtttgtt aaccagtttg tcaatgattc aaaaatcgtg aaattcgcat
    17881 tcaaggttgt ccaaggaatc aaactgatga tcatgatacc aaaagtaata aagaacaata
    17941 cattaacatg tttttgtttt ttcgtgatta cttcttgacg accgattgct tcaatatcga
    18001 aatgcttcat atcttcttta cgttggttgt atactaatga ttttgttgga tctttttcga
    18061 tctttgatgc ataacgatac acatatacaa tagaaatagc taacaaaata acgaacatca
    18121 agaatcgaag accaatacct tcacccatcg aaatcccaac agcttgtgaa gctacgcctg
    18181 ttgcaaaagg atttaccgtt gaagctagac agcctacttg agaccctact aatacgattg
    18241 ctacagctac tactgtatca aagcctacag ccatcatgac tgggatcagt aaagcataga
    18301 atggaatcgt ttcttctgcc attccgtaag tagagccgcc taatgcaaat aacagcatca
    18361 atactggaat aagcattttt tcttttcctt tgaatttctt cacaactgaa gcaatcccgg
    18421 catctagtgc ccctgttttg ttgataatac ctaaaaatcc accaacaacc aaaatgaaga
    18481 aagagattgg gattgcagca ggtgtttctt tagttagctc ccccgctggt acacctagca
    18541 tcccattgat tggcgccatg aaaatatccc acagcccttg aggattttgt tgtactcgtt
    18601 catatgatcc agcgacgatg ttgcctgcat cgtctacact atagtgccca gctggtataa
    18661 accaagtaag tacagcgatt actgcaataa taataaataa gaccgtatac gctgaaggca
    18721 tttgaaattt tttctttttt gtttgttcca tcttcttctt cctttctttt ccgttagaga
    18781 caacaatttt ggctctaatg ttattttatc aaaaagagta tcagaacaaa aataataaca
    18841 cgcattttgt aaaacttttg ttcaaaaagt tcgtttttcc ctattccctc ctcttttttc
    18901 aggtttttaa agctttcttc tttttcattg tagcgtttcc ttttttttat aaacaaacat
    18961 tttcgtcttt taaaaggaga agaatttcac atttgcaaag aattgattga tttttatgca
    19021 taaatcaaaa aaatactcaa aacaaacaaa taaaacgttt acattattgc atgtcttgta
    19081 tttttttcca aaaaaaaaga ccaagaaatt tcttgatctt tttgatattt atttgactgc
    19141 tgtatgacgt tgttcgaact gatgttgttt tcgacttaac catggaagaa gttgcgcatg
    19201 cagcactaaa aagacagcac tcacgatgaa tcctttgatc aagttgaaag gaacgatccc
    19261 aatcagtaca tagtttgcta aagacatgcc cagcatctgt gttgcgttca gccctaaaaa
    19321 cattaagtaa agaggcgtaa taacgaaata atttgcgata ctcataaaaa tagttagagc
    19381 gatggttcct gtaatgacac ctaaaaattt gtttcttcct ttatgctgtt tctttacact
    19441 aaagaaataa taaatcggga atgtaaagat cgttgttgat aagaaactag cagtgtctcc
    19501 taccaaatta tccggagaaa aacctgtagt cagcagatgt aatactgaac gcaagaacgc
    19561 tgttgcaact ccggccgctg gaccgaataa gaacatgctg atcaataccg agatatcact
    19621 aaaatcgatc ttcaaaaaac taaaagccgg catgatcgga aaagcaaaat actgtaaaac
    19681 taatgcgatc gcagcaaaca tcgctactgc aaccattttc tgtacacggg tgttcttcat
    19741 ttgaaatcct cctgttagga ctcctcttca gcgaactttt tgacgtttct ctccaaatga
    19801 aaaaactcta ttttccaggg aaaatagagt tagagttatt tgatcaagcg tcgtcaaata
    19861 agctctatct tctttatcca gactatactg tcggtatcgg aatttcaccg attcagctat
    19921 ttttttgcaa aataggtcgt ggactataac caccggtcgg gaatttcacc ctgcccctga
    19981 agacgaacct tatataattt ttgtttacat attcattata catatctatt caaaaaacgc
    20041 aacatctaac acttataaaa agtaagtgtt cattttgagt ggttcagcaa actgctgact
    20101 tcttttttgt tcagttcacg gtaatcccca ggacgtaggc cttgtaaagt cagatctccg
    20161 tatttttcac gttttagttt ttgcacagga agaccaactg cttgaagcat atttttcact
    20221 tgatgattac gtccttcgtg gatcgtcaat tccacgatac tgtggtttgt cttaggatcc
    20281 actgataaaa tttggaattt agctggagat gtttttcgtc cttcgatctt tattcctttt
    20341 gtcaaaggtg caagcatcga ttttgtagcg atccctttta cctttgcaac gtaaacttta
    20401 tccacttcat gttttggatg tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc
    20461 aagatccccg aagtatcgta atccagtcga cctacaggat agatacgttc ttttaccatc
    20521 ggcaaaagat ccgtaacaac ttttcttcct ttgtcatctg caacagcaga aataactcct
    20581 ttaggtttgt acaataaata gtaaccgtat tcttcttgat aaattgggac ttcatccact
    20641 tccacagtgt catgtttccc aacttttatc cctaattccc ttacaacttg tccgtttact
    20701 tttacacgtc ctgcaaggat gaattcttct gcttttcgac gagaagtgat gccagcgtgg
    20761 gcaattactt tctgcaatct ctccattaca attattctcc tttattcatt ccatcaaaga
    20821 atagatctgg tgacacgtct tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt
    20881 cttctaatga tttcaaacca aaataatcta agaagtagcc agttgttcca tataatatcg
    20941 gccgacctgg tccttctact ctaccttttt cttcaatcag tttatgggca gctagacgct
    21001 gtaccgctcc tgaaacctga actcctcgaa tctgctcgat ttcagctctt gtgattggct
    21061 gtttataagc gatgattgct aaggtttcga cggcggcttg agataatgtg tttgatgtcc
    21121 cttgcgcata tcgcttaagt aagggagcca gctctttttt agtcgttaat acataatggc
    21181 tttccgtttc aaaaatatgc aaagctgagt gcgagttttg agtgtaagat tcttttaatt
    21241 ctttcactaa acttgtgact tctgcttcgc tgagatttaa cagtccgctt aattcttcat
    21301 tactgatccc ttcctcgcca acaataaaaa gaagggcttc aagttcactt aatttcgtca
    21361 tgttcggtat ctcctactgc tggataaagc ataattgatt catagttttc tttttgttgc
    21421 gcaatgatct gccctttttt catcaattct agcaaagcca taaaagtagt gacaacttca
    21481 cttttagaag gtgactcaaa aaaagaatcg aaagtacagc catgcgcatt tctttttata
    21541 cgctcttcga taaataccat tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt
    21601 tccacttttt gagaaaattt cttacgttct aagattttct gcatggcacg gatcaaatcg
    21661 atttttttga cttgtcctcg cttcaacggc ttttcagcat ccgctaaccc atcgatgttc
    21721 ataggttctt ttgtgtaata aagacttcgt tcctctgctt tttctgtcag tctttcagct
    21781 gcatatttat atttacggta ttctaaaagc tgagtgacca attcttctcg aggatcttct
    21841 tcccataatt cttcatcgtc tgtgacgaat tcttggacag gaagcaatgt cttgcttttg
    21901 attgccatca atgtagcagc catcactaag tattctcctg caacagctag ctcgagtgtt
    21961 ttcattgtat ggatataatt catatattgc tccgtgatcg tagcgatcgg tatatcatat
    22021 atatcgattt ccatttgctg aatcagatga agcagcagat cgagaggacc ctcgaataca
    22081 tctaatttaa tattgatctc cgtcaactca ttcactcttt cttctttttc tttgctagcc
    22141 attttccaat gtaaggctct gtgatttcag ttgcacggat ggagacatcc gggtactttt
    22201 cctgtaattc atctaacatt tgaaagccca tattctcacc gcgataagac ggattcaaag
    22261 aaatgtcatg gatgatcagc tgatcgttcg ttgcccagta aataccaata atcccttgaa
    22321 tgttggttga accttctggc gtaaagaaaa aaagttcata atcgtcttca gattcgtacg
    22381 tatcaatctc cttcaacaaa gaagttttac ctgtcagttt cttatggaaa ctcaatagtc
    22441 ccatagcaat tttacggttt tctttccggt aatgtgttaa catcttctca ccttcctatg
    22501 actaagccct tggaaaatgc tgcttataaa catccgtcat tcttttcttc gtgatatgtg
    22561 tataaatctg agtcgtagag atatccgcgt gacctaacaa ttcctgtact gtccgcaaat
    22621 cagctccatt ttctagcaga tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt
    22681 ctttgtaaat cccagcctct cgtactaact gcttcagatt tttccagatt ccttgccgag
    22741 aaagaccttt cccatgatta ttgacaaata catgcatttc agatgcgtcc tttacaagta
    22801 atggacgtgc ttcgtctaag tagcgttcca gccattggat cgcataatca cctaaaggaa
    22861 tgatccgctc tttatctcct tttccaactg tctgtaccag tccaagagaa agatgaagat
    22921 ctcccaattt caatccaatc aattcactga cgcgcatccc agtagcatac atgacttcca
    22981 agattgcacg atctcttatc cctaacgttt ttgtcgtatc aggtgcttca atcagctttt
    23041 ctacttctgt cagagtcaat gtacttggta atttctgtgc tttcttcggc gtatcgatat
    23101 gttgcatcgg atcatgatca gtgatacgct cttgccgtaa aaactgatgg aatcttctga
    23161 gacttgagat catccgagta acagttgctg tcgctttttt ttcttcatgc aacttctcca
    23221 gaaaacggat gactaaaaag cgatcgacat cctgccatga agcaattttc tgctcctgta
    23281 gaaaatgcaa gtactgttcc aaatcacgtt catagctttg tcgtgtattg gcggacaatc
    23341 cccgttcaat attcaagtag tggagataat cgttgatttg ctctttcata ataatccccc
    23401 tcatgcctct ctatcatatc agataccgct tcattctgat agtctttttc cataatctga
    23461 gcaaagaaaa agccgtctag acactcagac ggactggttt cttttttctt tttcatagca
    23521 ttcctgacag attccatgga acgttagccg atgatctttt actaaaaaat gataacgttc
    23581 ttctactact tgctccactt caagtaaaag atcctcttct acttcctcga tcgtgccgca
    23641 gttcaagcat agtaaatgat gatggaaatg tttggcgcct tcttttctca aatcataacg
    23701 ggctaatcca tcattaaaac ttactttatc gacaattttc aattctgtca agatttcaag
    23761 ggttcgataa acagtagcaa gtccaatttc cggactcttt tttttcacta aaaagaagat
    23821 ttcttctgca gataaatgct ctttctcatt ttccaaaagc acaagcagag tagcttctct
    23881 ttgcggtgtc agcttaaacc ccgcttcatg tagctgctgt tttgtttttt tcatcgccga
    23941 aatagcattc atgaccattt actcaacccc aacaatttat aataattaca aataaaaaaa
    24001 gatataacac tatgttaatt aaaattatta taagctagtt aagaaaaaca tgcaagggat
    24061 tctcaattag attttttgac tagttttgtc acaccgtttt cttgtgtaat caatccttgt
    24121 ttcaaaagat tacctaatgc gcgcttaaat gctcctttgc tgataccaaa agctttcatg
    24181 atttcttctg gatccgattt atctgtaaag ttgatttgct gatctgcaga acgttctaaa
    24241 atggttaaaa tcatttgtgc atcatctgat atagcttcat atgctcttgg cttcaatgaa
    24301 agattcagta caccgtctgg tcggacacca atcactcgac cttttatctg ctcgcctaga
    24361 cgaggttcat tatatcgttc agaaggatgg ataaacccaa gatagaaatc ttccgttaaa
    24421 acgtacgtac ctgttaattt caagcggtat gctgttccta tcacatcttt gtttttcatt
    24481 tcttctgttc catatttgct taaagatttg aatatttttt catctgctaa actgccccaa
    24541 atacggtctt ttgcatcaac tctaagggaa atcatcaagc gatccccttt tttaggccat
    24601 agctctgtca ttgtcggcaa ttcatcaaga gatacgacaa agtctttatc tggaagaccg
    24661 atatcgacaa acgcacctaa atcttttcgt gtagcagtaa cgattccaaa accgtaatga
    24721 ccgatacggc ttttaggaat ttgtgtagtg atccgatttt catgtttttg attttggtaa
    24781 ccgaatcctt caactgcttc accgattttg tgttcacctt ctgatttgct taagccaaag
    24841 gtttgtccgt ttttttggac aaagtagttt ttttcatttt catcaataat aatacctgta
    24901 aatatagttg ctaataaatc gttcattttt ttctccctct atagtgaaag gttttcttaa
    24961 tacatttctt tgctatcata acataatgtt atctacttga atagctgagc cttggtttgg
    25021 aaaggtgaaa aaaggagtat gacaacagtt gtgtcatact cccctttttc gaataaacgg
    25081 tgttaaacgg caggctcaca gcctctttca tttgcctaaa attaattgct cccatttcct
    25141 ccgaaccatc cagttttaca gttgatggtt acgatgtaaa atggcaaagc actttctcca
    25201 atgcctttca tcaatgcaga ttttgttgaa gtggcatata gattataata gccttcccca
    25261 ttttcgattc tggctttttc taatctgtaa cctccttcaa cgatatatcc acgttggata
    25321 catgtagcaa tcactttgct ttggacacct ggtgcccacg tccatgctgg attagatgca
    25381 tcggcaatat cacttgctct ggcagatttg attggctgat tcgctgagtt tgaagaggaa
    25441 ggggtcgagg atgccgcttc ttgctgtgct gcttgtgcga ccgcagcttg ttgagcggct
    25501 tgagctgcag ctgctgcttg agcggcttgt tttgcttgtt cttcagctgc ttttttcgct
    25561 tcttctgctt ttttcttttc ctcttcttct tttttctctt tatcagtgag catcttatct
    25621 acttgtttca attgttcatt caactgagca gatagatctg tatttttcac tttttctact
    25681 gcttcttttg ccgtcttgta ttggtcgcgt gttgctgaat ccactacttt tccgtcttta
    25741 aagactatag ctgttttttc tttagctgtt tgcaattcag catattgatt tttagcttct
    25801 gtttgtgctt gttgaatcaa ttttccaaaa tctgtgttat ccgtttcgat tgcttggatc
    25861 tctttatctg ctttcaactt gatgtcatct gctagatgat catcgacgat ggcaggtgct
    25921 atgaaccact cattcacttt ttggatatct cggatcttag attggatatc ttggtaagtt
    25981 gtttctaact ctgaatatcc tttttcattc ttaagactgg ataaatcatt ttttagtttt
    26041 gataaatctt gatgaatcat atctgcacga ataaattgat ggtgatcatc tgtataaaag
    26101 gcagcaagtt tctcatgaag ttcttccatt tgttgctgtc tttttgcttc tgctgctact
    26161 tcggcttcta ctttttgatg atggtcataa taagcccagc ttcctccacc gagtagaagt
    26221 actgctgcta aaacgagata aggtactttt tttgattttt tcttttgcgc tttatctgga
    26281 tctgtttttt ctaagtttga ttcttgatgt gttttttcag tatctggcac gattgttatt
    26341 gatttcgctt ctgcttcaaa cagcttaggc tggttctctt ctttttctat ttttgcttca
    26401 agatcagtac cctctttgct tacagattga ttttcagcat cggattcact atttatttca
    26461 tcgggctctg ttgacttttt gtctgtgttt tcgagtattt cttttttctc tgcttccggc
    26521 agtttattcg ttttttcctt ttctaattgt ttttcagtaa atacttcagt tttttctgtg
    26581 atatccgttt ttacgtcttt tatttttact gtttctttac ctgtggcgtt ttctctatca
    26641 gagtcttttt cagtttcttg ttccttttct ggctctactt tgttttcctg tgcaattttt
    26701 tcactgatca ttttgagcag ctctttcgcg tgctcttctt ttgtatcttc acgatgtgct
    26761 ttgatataag cggatagtat cgaattttct tcctcattat cgacgatttc tttcagttca
    26821 gctactcttc ttttttgttt tgattcaagc gtttcttctt gattctttcc tgattcttcc
    26881 gaagtctcat gaacagttgc tttcttctgt tcatttttct ctatattagt tacttgatcg
    26941 tgagcaggtg gttcttcgtc ttctggttct gctttttcga ataattccat tacagattct
    27001 agaggtaaat ctttcaattc cgaccattcg attggatcgt ttaattctgt atcaggaaag
    27061 actgcatctt cagttgattt atcctgcata gctgcatctg tatttgtttc ttcaattgat
    27121 tcttttattt gctgatcttc tgcagttatt tgatgaccgc agtctggaca gatagtattc
    27181 tgctccattt ttttcgatcc gcaatttgga cattttttca tcacttagcg atcggctcct
    27241 ttactaggtt tacgctacca tttaatctat catattctat acgtcacgcc aatagcttat
    27301 ttttaggatt tacttaaaaa taatgaatga atttccttcc tatagaaaaa aaagcttgag
    27361 actgcgtagt ctcaagctt
//
LOCUS       DAPBCS010000039        27120 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPBCS010000039 DAPBCS010000000
VERSION     DAPBCS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.039"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            55..843
                     /locus_tag="RD521_001890"
     CDS             55..843
                     /locus_tag="RD521_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDT7771602.1"
                     /translation="MVLLESMPDGYIYSNILLKLYLRSLKHEGKLMFNDRIPFNSTML
                     ATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDIQSFIGKSSTEADRKRKYRKEI
                     EEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEKDIENVTPSKKSKAKPVRHKYG
                     EYKNVLLSDEQMEKLKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSE
                     SKNASSGYKRTGRREKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            855..1124
                     /locus_tag="RD521_001891"
     CDS             855..1124
                     /locus_tag="RD521_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771603.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1129..1293
                     /locus_tag="RD521_001892"
     CDS             1129..1293
                     /locus_tag="RD521_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771604.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1286..1588
                     /locus_tag="RD521_001893"
     CDS             1286..1588
                     /locus_tag="RD521_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771605.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1585..1746
                     /locus_tag="RD521_001894"
     CDS             1585..1746
                     /locus_tag="RD521_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7771606.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1743..2048
                     /locus_tag="RD521_001895"
     CDS             1743..2048
                     /locus_tag="RD521_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771607.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2048..2404
                     /locus_tag="RD521_001896"
     CDS             2048..2404
                     /locus_tag="RD521_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDT7771608.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2391..2609
                     /locus_tag="RD521_001897"
     CDS             2391..2609
                     /locus_tag="RD521_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771609.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2606..3025
                     /locus_tag="RD521_001898"
     CDS             2606..3025
                     /locus_tag="RD521_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDT7771610.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3022..3579
                     /locus_tag="RD521_001899"
                     /pseudo
     CDS             3022..3579
                     /locus_tag="RD521_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
     gene            3576..3872
                     /locus_tag="RD521_001900"
     CDS             3576..3872
                     /locus_tag="RD521_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771611.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3949..4362
                     /locus_tag="RD521_001901"
     CDS             3949..4362
                     /locus_tag="RD521_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDT7771612.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            4671..4823
                     /locus_tag="RD521_001902"
     CDS             4671..4823
                     /locus_tag="RD521_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771613.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(4820..5095)
                     /locus_tag="RD521_001903"
     CDS             complement(4820..5095)
                     /locus_tag="RD521_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771614.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            5549..5755
                     /locus_tag="RD521_001904"
     CDS             5549..5755
                     /locus_tag="RD521_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771615.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            5951..6118
                     /locus_tag="RD521_001905"
     CDS             5951..6118
                     /locus_tag="RD521_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDT7771616.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            6144..6488
                     /locus_tag="RD521_001906"
     CDS             6144..6488
                     /locus_tag="RD521_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDT7771617.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            6493..6774
                     /locus_tag="RD521_001907"
     CDS             6493..6774
                     /locus_tag="RD521_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771618.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6877..7191
                     /locus_tag="RD521_001908"
     CDS             6877..7191
                     /locus_tag="RD521_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDT7771619.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            7169..8863
                     /locus_tag="RD521_001909"
     CDS             7169..8863
                     /locus_tag="RD521_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDT7771620.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8883..10061
                     /locus_tag="RD521_001910"
     CDS             8883..10061
                     /locus_tag="RD521_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDT7771621.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            10024..10710
                     /locus_tag="RD521_001911"
     CDS             10024..10710
                     /locus_tag="RD521_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDT7771622.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            10710..11870
                     /locus_tag="RD521_001912"
     CDS             10710..11870
                     /locus_tag="RD521_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDT7771623.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11880..12755
                     /locus_tag="RD521_001913"
     CDS             11880..12755
                     /locus_tag="RD521_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771624.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12752..13063
                     /locus_tag="RD521_001914"
     CDS             12752..13063
                     /locus_tag="RD521_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771625.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            13053..13406
                     /locus_tag="RD521_001915"
     CDS             13053..13406
                     /locus_tag="RD521_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDT7771626.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            13396..13797
                     /locus_tag="RD521_001916"
     CDS             13396..13797
                     /locus_tag="RD521_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771627.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13790..14194
                     /locus_tag="RD521_001917"
     CDS             13790..14194
                     /locus_tag="RD521_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771628.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            14206..14814
                     /locus_tag="RD521_001918"
     CDS             14206..14814
                     /locus_tag="RD521_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7771629.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGAAEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     SEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVATTATQSQ"
     gene            14834..15196
                     /locus_tag="RD521_001919"
     CDS             14834..15196
                     /locus_tag="RD521_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771630.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            15199..15381
                     /locus_tag="RD521_001920"
     CDS             15199..15381
                     /locus_tag="RD521_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771631.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            15399..18719
                     /locus_tag="RD521_001921"
     CDS             15399..18719
                     /locus_tag="RD521_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7771632.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SSVFDNLNISIPHIPLPHFKLSGEFNPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAG
                     PEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            18770..19507
                     /locus_tag="RD521_001922"
     CDS             18770..19507
                     /locus_tag="RD521_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7771633.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19517..21808
                     /locus_tag="RD521_001923"
     CDS             19517..21808
                     /locus_tag="RD521_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDT7771634.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDSIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21832..23934
                     /locus_tag="RD521_001924"
     CDS             21832..23934
                     /locus_tag="RD521_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771635.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSINFGDYDYSGNPNLLSKLSYDLIE
                     NQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTLVMTVKLRAGA
                     DYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTTPTFSPQAYHP
                     WIQFRAQDGILGEIEMSYDIKIEEGSTATPFQPNLLAEPYNMCREYPNENIADHTVKF
                     PIESGDHQIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLT
                     DVWSLTFTPTNVVASSPKLFRVYQYPQSTVGACRIDWLKIEKGDTRTPNISEYKYRGT
                     GMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINE
                     VDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVS
                     IYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKW
                     RRNSSYGSDYTFAITYTI"
     gene            23951..24100
                     /locus_tag="RD521_001925"
     CDS             23951..24100
                     /locus_tag="RD521_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771636.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            24097..24543
                     /locus_tag="RD521_001926"
     CDS             24097..24543
                     /locus_tag="RD521_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771637.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24545..24682
                     /locus_tag="RD521_001927"
     CDS             24545..24682
                     /locus_tag="RD521_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDT7771638.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            24720..25013
                     /locus_tag="RD521_001928"
     CDS             24720..25013
                     /locus_tag="RD521_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771639.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            25010..25234
                     /locus_tag="RD521_001929"
     CDS             25010..25234
                     /locus_tag="RD521_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDT7771640.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            25231..26256
                     /locus_tag="RD521_001930"
     CDS             25231..26256
                     /locus_tag="RD521_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDT7771641.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            26446..26519
                     /locus_tag="RD521_001931"
     tRNA            26446..26519
                     /locus_tag="RD521_001931"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26480..26482,aa:Ile,seq:cat)
BASE COUNT         9623 a         4122 c         5744 g         7631 t
ORIGIN      
        1 aacaaacgat actactattt aaaactaaaa gagaactttt tcgatagcga cgagatggtt
       61 cttttagaaa gtatgccaga tggatacatt tattctaata ttcttctcaa actttattta
      121 agaagtctga aacatgaagg caaactaatg tttaatgaca ggattccatt caattctaca
      181 atgcttgcga ctattacaag acactctgtg ggagttgtag aaaaagcagt acaaatattt
      241 cgtgatttac aacttattga ggtattagat aacggagcaa tttatatgtc tgatatacaa
      301 agttttattg ggaaatcttc aactgaagct gataggaaga gaaaatacag aaaagaaatt
      361 gaggaagcaa aacagaattt aataacaagt ggacaaatgt cggacaaatg tccggacaaa
      421 actacaccag agttagagat agagttagag aaagatatag agttagagaa agatatagag
      481 aatgtaacgc cttcgaaaaa atcgaaggct aagcccgtcc gtcataaata cggagagtat
      541 aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaatagaatt ccctaatgac
      601 taccaagagc gaatagaacg gctatctgag tattgtgaat catctggtaa gacttataaa
      661 aactatttgg caactattcg aagctgggca agaaaagaaa aaagtgaatc taagaacgca
      721 agcagtggat acaagcgcac aggaagacga gagaagcttc ctgagtgggc aatcgaccaa
      781 gaagcctatc tcaagaaaaa agcgctagaa cgagctaata gacaatcaaa agcaccattc
      841 taagaggtgg gaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
      901 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      961 tcgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
     1021 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
     1081 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
     1141 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
     1201 gaagacaagt ggctcaacac gttccacggc ggacgtgaga gcgcacttga tcaagtactc
     1261 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
     1321 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
     1381 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
     1441 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
     1501 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
     1561 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
     1621 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
     1681 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
     1741 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
     1801 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1861 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1921 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1981 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     2041 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     2101 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
     2161 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
     2221 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaattg
     2281 gagcaagaaa cttcatcaag atcgtttcaa atttgtagag aagtataaag aaatattaga
     2341 agagtatttg aggaggacag caaatgataa aaaaactcgt tcaattcagc atggatttat
     2401 atgatatcga atcaggagca acactatctg tggaatcgga ccatctaatc ataaattttg
     2461 gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt actgtttcca gaaattgttc
     2521 atgatttcga agaatcaaaa gcggttgagt ttgaaatagt gaaaaaagta atggaattga
     2581 ttgaaaaata cgaggaggac agcgaatgat accgaagttt agagcgtggg ataaacgaaa
     2641 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
     2701 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgacctcat
     2761 gcaatccaca ggaatgaaag ataagaatgg tgtggatata tttgaagggg atatagtatt
     2821 agtcagcgtg cgaaatggct tcgattactt agataataaa gtctgtattg tcaaaaattc
     2881 aatagattat tccggattag tttgtgccac tgttgatgaa gacttagagt atcaaatttt
     2941 taacacagag ctgtttgaag aatacacgta cgaagtcacc ggaaatatat acgagaatag
     3001 cgagttattg gaggaacagc gatgaataaa caggaagcaa tcaaaaagtt agaaagtatt
     3061 aaagcgatag gaaatgatgc aatagctgct tgctataacg agagtataaa ttcaggtatt
     3121 acgttaatga aaaaaataga cgaaccgcag aaaccagtta ttccacaact cgtggccggt
     3181 tggcttgaga aatctacgga cccttttaca aaagctgaaa aaatagcgta tttaatcaaa
     3241 tctaaagatg gtgattcata ttatttctgt gattggtttg tacgagatgg catagtgacg
     3301 caagagcaag gagaagaatt actcgcttgg gcaacaagac aatcatatga aacactattg
     3361 agcctataca acggctaaga ggttgagaaa gagccgttat gggcaataaa gaatgccgat
     3421 ggaaactatc ttactaaatg tgctttatgg ggaaaagatg gagtaaatta tagttttgaa
     3481 tgcaatccat ctcatcgatt gcttttcact gataaagcaa cagcggatgc tgcagcattg
     3541 ttggtgaatg gaacagtgga agaggtggta gaaagatgaa actaaaagac ggattttacg
     3601 ctagcagtca cggtatcggc ggtttaatgc tagatatgcc gacaaagaac cctaaaacac
     3661 gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg tgaagcagaa gagttcgttt
     3721 atccgttcag aggatacgtt aaaaagatac tgtcaaactc agcaatcatt cgtattgaaa
     3781 atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca ttatttagct gtagcgagat
     3841 tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc ggatcgctcc gactgattca
     3901 ataaatccaa cacatttatt atatcacata aaggagcggt ttgacttgat gcaattgtta
     3961 cgagaggtag atttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
     4021 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tataacggat
     4081 atgccgaagg caccgaagca tggcaataag gcagaagacg cgattattca gatgatggat
     4141 atagaaacgg agagagatgc gattctagcg gctttgatgg ctcttagtct gattagtcgt
     4201 cagatactct actacagctt ctgtgtgcca gatagcttct caaactacag aattagccgt
     4261 gaagtgggtt attcagaaag aagtatacaa cggatgaagt cggaagctct aatagagttt
     4321 gcagaagcat ataaacacgg aaaaataatt gcttataaat aatttggcgg ttttttggcg
     4381 gaatgatggc ggtttttaga catttaccag tgttattatg gtagtgtcga aagattagga
     4441 aacaggatcg acaaaataaa cgaagggagg caatctccct catcgtttta aacttcttct
     4501 tagatagaca gcaactaatt atgaagaaaa ggatgtgaat tcaactcctc ctaaattctt
     4561 catcggttgc tgtttatttt gattagacag cagcactcac aaaactaaac atgttcttgt
     4621 cgtctttcaa ctgctgctgt ctattaattt atgtattgga gggaagaaaa atgaaattat
     4681 cagtagaagg cactccagaa gaaatacaag aattgctcca agctattggt agtagcaagg
     4741 agcaaaaggt aagtgtttct aattcagata ttgacggtat ttgcaattgt ttaaacagag
     4801 aaaaaatatc taatccttac taagagaaaa tatgttcgtt aatgaatttc caattttcct
     4861 ctgtagctct accttgataa tttttttgca gttcacagat gaataaggaa tcgttattat
     4921 caagtactgt atttaatttt tcacacattt gatttggatt caaattagtt ttaactaacc
     4981 aaaaagattt ttgtagcttt atatatgccc caaattcttt tattatttca aatactttat
     5041 catacttttg ccctgggtta ttaagatcat aactaatcat gtagggttta tacatgttgt
     5101 cttgcacatc cttgttttat atttcagcgg accactcgct gataaataaa attatacgct
     5161 tagtatttat tttcacaata ttaatttatc gtaataactt ttgagtaaat agaacaaaaa
     5221 acctgcacta gtttccgcta gtacaggcag tgactatatc ggttgataat ctagcatata
     5281 ttaaaaataa ttgcaagaaa agaaatttat agtggtttat accaaattat caaagcaatg
     5341 ttattttgtt gctgtctatt gttttttaat tattcacacg ataactaaag gtgggtgaag
     5401 agaaatgatt ccattaataa tttcaatttt tgcgctctgt cttaatgtct atatgattgg
     5461 atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat agtgtcactg
     5521 tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca accatgcaag
     5581 gttcgattcc ttgccagcga cattaaatgc ctatgacggt tacgactacc gaaaaaagat
     5641 cgttaagaag ctatacggtg ctacgtacgg caatgtagta agtgtgctat ctgtacacca
     5701 ccaagcttcg gtcactgtgg cggaagtaga agacgcagcg gtaaatggcg agtagcctcg
     5761 tgagagcctg gtaagttctc gtgagtggtg caatcccact ccagcgactt taagcaaccg
     5821 aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg
     5881 caagttacta ttacatatta gatcactctt tgagtggtct ttttattttt gcacaaagga
     5941 ggaaacaaca atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgttca
     6001 taaaggtaat ggcgaaaaag tatatgagta tagaagacca ataaagagcg atacatataa
     6061 acgaaaggaa aacaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
     6121 aagattgcga aaggagacgg aacatgaccg aggaattcta tagatggcta ttacagttga
     6181 caagagaaga tcgtttggtt aagttctatc agtctcctaa atggcgcagg cttagagaga
     6241 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
     6301 gagtagagaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
     6361 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
     6421 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
     6481 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
     6541 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtaggt
     6601 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
     6661 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
     6721 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
     6781 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
     6841 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
     6901 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
     6961 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
     7021 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
     7081 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
     7141 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
     7201 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
     7261 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
     7321 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
     7381 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
     7441 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
     7501 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
     7561 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
     7621 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
     7681 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
     7741 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
     7801 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
     7861 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
     7921 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
     7981 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
     8041 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
     8101 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
     8161 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
     8221 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
     8281 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
     8341 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
     8401 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
     8461 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
     8521 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
     8581 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
     8641 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
     8701 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
     8761 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
     8821 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
     8881 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag
     8941 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
     9001 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
     9061 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
     9121 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
     9181 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
     9241 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
     9301 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
     9361 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
     9421 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
     9481 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
     9541 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
     9601 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
     9661 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
     9721 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
     9781 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
     9841 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
     9901 atggagtatt tacaatcaac gaaggaagag aacgcgttgg aaatgatcgc tctgataagg
     9961 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
    10021 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
    10081 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
    10141 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
    10201 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
    10261 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
    10321 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    10381 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    10441 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    10501 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    10561 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    10621 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    10681 aaaacatttg gacaaggaga tgcaaaataa tgacaattaa aaatttaaaa ggtgtaacag
    10741 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    10801 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    10861 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    10921 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    10981 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    11041 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    11101 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatggc tttgatgtaa
    11161 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    11221 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    11281 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    11341 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    11401 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    11461 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    11521 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    11581 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    11641 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    11701 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    11761 atggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    11821 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    11881 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    11941 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    12001 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    12061 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    12121 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    12181 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    12241 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    12301 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    12361 caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa ttattggaaa aaggtttatc
    12421 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata caaatctgga
    12481 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    12541 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    12601 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    12661 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    12721 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    12781 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    12841 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    12901 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    12961 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    13021 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    13081 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    13141 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    13201 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    13261 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    13321 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    13381 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    13441 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    13501 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    13561 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    13621 gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat tcaaaatcgt
    13681 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    13741 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    13801 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    13861 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    13921 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    13981 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    14041 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    14101 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    14161 tctgattcgt caattcgata aattaaataa atagggggaa caacaatggc taaaacaaaa
    14221 actgtagtaa caacgttcga taacataagt atcaaacgaa ttgcttttaa ttttaagaac
    14281 gcaggaaatg caatcgcaac gaattgtaac ggacaattag atggcgaaac agaaatgcaa
    14341 acggtggtta aaaaatgtgg agcggcagaa gtaaaatcaa aatctaaacc aatcaatatg
    14401 acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa
    14461 caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt aagcgaagat
    14521 ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt agttggtatg
    14581 ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta
    14641 gctgcgttag aactagaagc aaaagttatg caagatgaat ttggtaaatt ttatcatgaa
    14701 gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct
    14761 gatgtgatta aaaaaacttc agttgtggca actacggcca ctcaatcaca gtaaaaaaaa
    14821 cggaggtagc gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt
    14881 gaatttagaa cctaaactga atatcaagaa attattgatg atcaatagag attttaacac
    14941 agacgagttt gcaaaaatgt cgatgggaaa aggctctatg gatattacag ttattcaagg
    15001 tgcaaaagcc gtatatgtag cttatcgtca agcgaacatg gtcgattaca tttcattcga
    15061 tgaatttata gataaatggg attttgatat ggaggttgca gtagctgtat acagtactat
    15121 gatgttcaaa caagcacgtg atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa
    15181 aaagcttcaa aagtaaaaat gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg
    15241 atgttgaccg acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat
    15301 gacattttgc aaatggctta caacaagagc gcttatgaag gatggaagaa ctatgcaata
    15361 aatcaatccc agaaaaacta aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag
    15421 cagaagtaac tttcatagct aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg
    15481 ctgaattaac taaaaataga gcagaattaa aactagaaca agctcaatta caacagactg
    15541 gttctgaatc agacaagtta ggaagtaaat tatcttcttt agaaaagcag tatgaattac
    15601 aaagtcaaaa agttgaagta actagtcaac gtttagccaa tgccaaaaaa tattatggag
    15661 aaaattccac cgaagttcag aaacttgaaa gagaactgat taatcaacaa acagctcagc
    15721 aaaggttatc taatgatata gataaaacga gtaaggcatt ggctcaatct aaaggtgaat
    15781 ttaaaacgta tgagtcaact atgaaagact tagataatga gcaacaacat ctaaagtcaa
    15841 gcgcatcatt agtagaatcc gaatataaaa aatggcaagc aactgctggc cagtctgcta
    15901 cagaatctga aaaattagca aaagcacaag aatatgtcgg caaacagagt gatattgctg
    15961 aacaaaaaat cgaagtgcta aaaaaacaat tagatgccac acaaaaagaa tttggagaaa
    16021 catctaccga agctttagaa atgaaaacta agcttaatga tgcggaaaga gaattcgaag
    16081 aattaagtaa tgctgctaaa aacgttgata cctccacagt agatgatatc ggcaaaaaac
    16141 ttgatatggg taatttaatg gaagctactg atcacttgtc agtgatcgga gataagctta
    16201 ttgatgtagg tagtaagtct attgaagcag ctggaaaagc acaagctatg caggcccaat
    16261 ttaaacaagt ctttggctct ttagaagggg aagcacagga cgccgttgag ggaatggctg
    16321 aagaatttgg aatgttacca aatacgatca agcctgtttt tacacaatat acgtcaatgt
    16381 ttaaaggact tggatatgat accaaagaag ctatggagtt agctggtgat agtactcagt
    16441 tagcagcaga tgcagcagct ttttacgata agtctatgga tgatgctagt gaatctctta
    16501 attcatttat aaaagggaac tacgaaggtg gggagcaaat aggtttattt gctaatgata
    16561 ctcaaatggc agcttatgct gttaagcata atttgatacc agcgactgaa ggagcaaaaa
    16621 aagccagtga agagtcattg ttagctgttg aaaaagcaca atctaagtat gctgatgcta
    16681 ttaagaaaca tggtgaagga tctttagaag caagagaggc tgctttaaaa cttaaagatg
    16741 cgcaagataa aataaatgaa gaattaggcc cacaaacgca aaaatggtct gatttagatg
    16801 aggctaccaa gcaagcagtt cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg
    16861 ctacaggtca ggctagtagg gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc
    16921 aagcgttaga agatttttat gcctcattgg gtgaagatat actgcctgtg tttatcaaag
    16981 ggttgcaagc aggagcgaaa gctttgcaag gattagccga atggtggagt aaacttgatg
    17041 ggccaatgaa aaatttcatt ttagctcttg gaggaattct agcattatta agcacattag
    17101 ctcctgttat aaccgcagtt gttacgatag ttggcacatt tggttctaca gttttgctgc
    17161 caataatagg aattatagcg ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc
    17221 aaaactgggg cgcaatcact gactggttta gtgatttatg gaaaaaattt accgattggt
    17281 taggtgatac ctgggaaagt ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag
    17341 aaacctggtc tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt
    17401 ttggagagtt atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag
    17461 agacttggca agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat
    17521 ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt
    17581 cattgattca agctggtatt aatattatcg tgggtatttt tgatgtagct ggacagttat
    17641 taggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg
    17701 atactatgac acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta
    17761 tatttgatcc agtagtggca tggtggaatg gtatatggac atctattagt actacggctt
    17821 caaatatttg gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata
    17881 caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat
    17941 ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta
    18001 gcgtgactag cagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt
    18061 ggaatagtat atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga
    18121 atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa
    18181 tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa
    18241 gcacgatagc taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga
    18301 ttagagataa aatcagtagt gtctttgata atttgaatat ttcgatacca catattccgt
    18361 taccacattt taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg
    18421 gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtcggtg
    18481 aagcaggacc tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggggatc
    18541 gtatcttgag tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact
    18601 acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc
    18661 aacaaatcat tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa
    18721 caggagagta tttctcctgt ttttttagtt ttaaaaagga tgtgaaaaaa tgactgattg
    18781 tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt tagcggaaag
    18841 accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag gacggctagg
    18901 tggcttagta caagattact cgtttgaaga catgtcgttt acattgcact ataactattt
    18961 agaagatgtg gaagaccatc aagcattcaa gcaatcgttt tatatcatgc gtcattggtt
    19021 aaactatgca aagaaattag aattctctga tgatcccaac gtctattatg ttatccagac
    19081 tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat
    19141 cactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca
    19201 gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc
    19261 ttcagctact tcatgccaat tcaccttaaa tgattatgtg tttagttttg aaggcttagt
    19321 agtgggaact gacatagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga
    19381 cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccaa cgttgcaagt
    19441 ggatattaat cattttaatt gtactggttt gagcaaaata caaatttatc gtaatgggtt
    19501 aaggtaggtg aaatagatga tcgataattt actaactatt tacgataaaa atgacgcgaa
    19561 taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac
    19621 agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt attcaataaa
    19681 cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa
    19741 tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat
    19801 agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga
    19861 cagcggaaca gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa
    19921 accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa
    19981 gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg
    20041 atttcatggt gagtttctaa aagataataa cacattacgt catgaaaaaa gtctaggtac
    20101 ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga
    20161 tgctcaaagt gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga
    20221 cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg
    20281 atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc
    20341 tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat
    20401 tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt
    20461 ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag
    20521 aattgtggaa tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa
    20581 cgtcaaaaca aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgattc
    20641 cattgatcaa ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca
    20701 tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc
    20761 gcctagtcgt attttgatta tggacacgcc agataagaat accgcacgga atgttttaca
    20821 aatcaacaac gctggtattg gtttttctaa aactggtatt aatggaacat atgaaaccgc
    20881 atggacgtta gatggcggat tcaatgcctc gtttattaca gctggtgaga tagtagggat
    20941 tactattaga ggtactacat taattagtga tggtgctgat tatagaacaa gtatagctaa
    21001 tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg
    21061 tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt
    21121 catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag
    21181 accgtttcta tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc
    21241 tggcgacggt agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt
    21301 agctggtagg actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt
    21361 tggttcatgg gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa
    21421 agattttact gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg
    21481 actattgaac gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac
    21541 aggtgacgat ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct
    21601 aagtcggtat atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga
    21661 agatattgac catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt
    21721 agtggcacac cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag
    21781 cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc
    21841 agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg
    21901 gctcgtcagg gtgataaagg gattacggtg acggttaaac catatttaaa tggcttgcag
    21961 atggatacga gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta
    22021 gatagtgttg caactagcgt aactagtgaa gaagtcacgt tttctcttga tggcacattt
    22081 atgagtgaag caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt
    22141 ctaacaacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggtcaa
    22201 gccgatgaat atgtttcgca attagaagag ttgattcgaa agtataatga aacttttgat
    22261 gcttttatgg ctgaaactaa aggtagagtg gatagcttaa atcaacagat tactgattta
    22321 actggtcaag ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag
    22381 ttaggtaact tacaagtgat gtacagtaac agcatcaact ttggtgacta tgattatagt
    22441 ggaaatccta atttgttaag taagctatca tacgacttaa ttgaaaatca aaatacttca
    22501 gctggaacac tttctaaagg tgaaaactcg tttaaatata ataagatatc agctgaagtg
    22561 gaaggtggag tagagctata ttataaacga agaggtatag ctaactggct accatctaat
    22621 aaaacgcttg taatgactgt taagcttaga gctggagcgg actatagtcc agttgacgga
    22681 aaacttatac tgattagata taggtatgtt gacagtggaa ctggcaagat tgttttagac
    22741 ttacctatta acagtaattc gataactcag gaatggaaag agtttagtat tactggaact
    22801 actccaacat ttagcccaca agcataccat ccttggatac aatttagggc tcaagatggg
    22861 atacttgggg aaatagaaat gagctatgac atcaaaatcg aagaaggctc aacagctaca
    22921 ccattccaac ctaacttatt agcagaacct tacaacatgt gtcgcgaata tcctaacgaa
    22981 aatattgccg atcatacagt taagttccca atcgaatctg gcgaccacca aatatatcaa
    23041 ggttacacag aagaagagct tatgataggt caaacgtata ctatcacgct taaaggaaca
    23101 aaacccgcaa gtcaaacctt tgtagcgtat aatcattgga ctgctcgttt aggagaacta
    23161 aagccagttg atgggttgac agacgtatgg tctctaacat tcacaccaac gaatgttgtg
    23221 gcgagttcac ctaaactttt tcgtgtttat cagtatccac aatcaacagt aggcgcatgc
    23281 cggatcgact ggctcaagat tgaaaaaggc gacacacgaa ccccgaatat tagtgagtat
    23341 aaatatcgtg gtactggtat gcgtgattca aacaatccaa aagattatgt ttgggatcta
    23401 gcaccagaat atgtcgaaga taacttggcc acagatatta aaatttctga aattactggt
    23461 aaagcaaaca attataccga tgggaaagta tcggagatta attcgcagtt gactgcttca
    23521 attaatgaag tagacaccac agctaaggat gctcaaacaa aagcgaatgc taatgcgact
    23581 gctatagatg aattagacaa taagatcgat gaacgcatta atgatacagc tactaccaca
    23641 ttaacagtta caaacgggaa taccggatca gcaaagcttt atcgtgaagg aaaaacagtt
    23701 tctatatatt ttgtggcttt aaacggaaaa agcagtggtg gaaatgattc aacgatacta
    23761 acaattccag aaggctatcg gccaccaatt agttttgagc aactggttgg ctcgatagac
    23821 cgttctactt tgaacagtgc tcagttatct attggtgcag atggagccat taaatggcga
    23881 agaaactcaa gttatggatc ggattatacc tttgcaatta cttacacgat ttagaaaagc
    23941 gtgaatcgat atgaaggcag catatagacc aattgaacct tacggattcg agcaaatcat
    24001 tgtgaatgat gaagaacatt taccggaaga atgcacagaa gtcgaaccac cgattccaaa
    24061 ttggaaaccg aaattcaatt actgggaggg aaataaatga aaaacatttg gaaatatgga
    24121 cgtactggcg gagagtacgc aggaaaagta ttggacgaca tgcttgtatc cgttccttac
    24181 acagatcagc caccgcttga aggaattcgt gctgatggcg aaccgttaac aatcgctgat
    24241 caaatgtttg atcctaaatt gaatcaatgg attattttag cgaacgcact agatcacaac
    24301 gatttaaaca atctcaaagc aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa
    24361 cagatcaatg ccaaactcat gctaagcgat gtagcaatta aacaggaaaa tactgcattg
    24421 aaagaaaaag cggatagttt agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa
    24481 aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa gggaggtgag
    24541 tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt tcactaacga
    24601 acaagtaatg gttttcgttc cgttgtgcat tactgaagaa gaagcagata aaatcattag
    24661 caaagaagag agcgcatctt aattgatgtg ctttttattt tgattcaagg agttgtcaca
    24721 tgattaattt aggggaatgg ggagcgatag caggatcaat aaccgctatc gtttctttga
    24781 ttttattagt aataaaacca attactgcat ctttctcgaa gattactgag actctttcaa
    24841 aagtaagtca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
    24901 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat gcagaaaaat
    24961 tggtagaaca cacgcaacaa attaaaactt tatttaggga gagaagaaaa tgaataataa
    25021 aacgttcgaa gtactaaaat ggttcgcact ggtaattatt cccgcactag ctactttcgt
    25081 ggggttagtt ggtaaagcgc tcaattggca gtacacagat atctgtgttg tcatcattac
    25141 tggttttggc gcgtttttag ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt
    25201 ctcggctgaa agcgaagaag gaggaaacaa atgaaaaaga aaattactat tactgcgatg
    25261 agcctgttaa cggctctttt tttattaccc attaatacgt ttgcttatac tattaatgac
    25321 gagtataatt tagcgccgaa tcaaggagac tccagattag caattcctaa caaaattatt
    25381 ttgcacgaaa ctggaataga tgcaccagca agaaacgtag ccgccaacat gaaaaataat
    25441 tataacggaa gcaatcctta tactacagat gttattggtg acggtgggat tgtttaccgt
    25501 gtgggtgagc aaggatatgt ttcgtgggga gctggtaacg ctaatcctta tgcgcctgta
    25561 cagattgaat tacagcgcac atatgataaa gcattgtttg aaaaaaacta tcgagcttat
    25621 attgaatata caagagatag tgcaaaaaaa tatggaattc cattgactct tgatcaagga
    25681 acttctttat ttacaaaagg aatcatttct catttgtggg tgacaaatta tgtttggggg
    25741 aaccacacag atccatatgg ttacttatcg caaatgggag ttagcaaaga aaagcttgct
    25801 tatgatttag ctcatggatt taccgatgaa aatccaacaa cttctgaaaa caagcctgtc
    25861 attgatccaa cacgagctgg tgcagctaat cctacgctga cagatggaac aaatcacgcc
    25921 cacattgatc agtttgggga aatcgaaaac gcaaacttgc atgtggctgg atggcacatt
    25981 gctaactata aatacgagta tattttcatt atggactaca atactgggaa agaattagct
    26041 cgagtaagag ctgatggaat ttatagacca gatgtaaacc aagcttataa tacttctgga
    26101 aatgttggtt atcatgtatc tttcaatatg cgtaattttc ctagtaagaa agtctatgta
    26161 atgatgcggg caacgaatga tccagaggga aacactaaag gcggtgcgca agatttccat
    26221 gacaaacgtt ggtatttaaa tattcctaaa cgataaaaat agctcctcgt tgaggagcag
    26281 tacataacta tattgacaac tataaaaatt attcgataaa atagtgatgt tatcgcatat
    26341 cttcactatc acccataaat agtcacactc caagctatgc gataacaggt ttgttgccac
    26401 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
    26461 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
    26521 taacgtagcc atttgaatcg ttctgtgtta gaattttttt gaagagtatt atacaagcta
    26581 aagcttttct tcattgccac tcaaatgagt ggctttttta tgtatccttt tatggattaa
    26641 tgaaaggatg tttcacatag ttatacttct gtatatttga aaagttttac tttgattttt
    26701 aaatagaaag acatttgggt taaattgtga gataataata aagaagagtt taaagcgcac
    26761 cccaaaccac ttccccataa gtgtgttacg ctttaaactc ttttatattt gaagccatta
    26821 aaaagcatac catatttttg aaaaaaagtg agaaaaaagg cttacaattg gagtggtagt
    26881 taattagtga cttatttttg attttatagc actgatacta taaaatatag atatcatcat
    26941 attacacaat cttaatacta acttaaaaaa tatctccttt cacaagtatg gtgataaaat
    27001 tcgttccggg ctaccttttt aggtagccta ctttaatctt tatacctttc tggatcaacg
    27061 aaagtatact ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc
//
LOCUS       DAPBCS010000040        27081 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPBCS010000040 DAPBCS010000000
VERSION     DAPBCS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.040"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..641
                     /locus_tag="RD521_001932"
     CDS             <1..641
                     /locus_tag="RD521_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7771642.1"
                     /translation="TGAGKTTIIKLLMREYDDYQGKIQFGGRSIKEYSLNALLGSIGY
                     VPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLAFINNEIKELPKGYDTLVGERGVS
                     LSGGQKQRISIARALIKNPELLILDDALSAVDAKTEEAILHNLKEIRKEKTTIIAAHR
                     LSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYEQMWEKQQLEAKIEGGEA"
     gene            641..2425
                     /locus_tag="RD521_001933"
     CDS             641..2425
                     /locus_tag="RD521_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771643.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            2751..3602
                     /locus_tag="RD521_001934"
     CDS             2751..3602
                     /locus_tag="RD521_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7771644.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            3656..5407
                     /locus_tag="RD521_001935"
     CDS             3656..5407
                     /locus_tag="RD521_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7771645.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            5411..5581
                     /locus_tag="RD521_001936"
     CDS             5411..5581
                     /locus_tag="RD521_001936"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7771646.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(5621..6523)
                     /locus_tag="RD521_001937"
     CDS             complement(5621..6523)
                     /locus_tag="RD521_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7771647.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            6754..8157
                     /locus_tag="RD521_001938"
     CDS             6754..8157
                     /locus_tag="RD521_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7771648.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            8157..9590
                     /locus_tag="RD521_001939"
     CDS             8157..9590
                     /locus_tag="RD521_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDT7771649.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            9735..10661
                     /gene="trxB"
                     /locus_tag="RD521_001940"
     CDS             9735..10661
                     /gene="trxB"
                     /locus_tag="RD521_001940"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDT7771650.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            10875..12602
                     /locus_tag="RD521_001941"
     CDS             10875..12602
                     /locus_tag="RD521_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDT7771651.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            12617..12967
                     /locus_tag="RD521_001942"
     CDS             12617..12967
                     /locus_tag="RD521_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDT7771652.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(13059..13919)
                     /locus_tag="RD521_001943"
     CDS             complement(13059..13919)
                     /locus_tag="RD521_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDT7771653.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            14011..14439
                     /locus_tag="RD521_001944"
     CDS             14011..14439
                     /locus_tag="RD521_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDT7771654.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            14602..14778
                     /locus_tag="RD521_001945"
     CDS             14602..14778
                     /locus_tag="RD521_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDT7771655.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            14805..15251
                     /locus_tag="RD521_001946"
     CDS             14805..15251
                     /locus_tag="RD521_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDT7771656.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(15405..16784)
                     /gene="brnQ"
                     /locus_tag="RD521_001947"
     CDS             complement(15405..16784)
                     /gene="brnQ"
                     /locus_tag="RD521_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDT7771657.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            17490..18461
                     /locus_tag="RD521_001948"
     CDS             17490..18461
                     /locus_tag="RD521_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDT7771658.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            18485..20677
                     /locus_tag="RD521_001949"
     CDS             18485..20677
                     /locus_tag="RD521_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDT7771659.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            20694..21170
                     /gene="ybeY"
                     /locus_tag="RD521_001950"
     CDS             20694..21170
                     /gene="ybeY"
                     /locus_tag="RD521_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDT7771660.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            21154..21549
                     /locus_tag="RD521_001951"
     CDS             21154..21549
                     /locus_tag="RD521_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDT7771661.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            21564..22463
                     /gene="era"
                     /locus_tag="RD521_001952"
     CDS             21564..22463
                     /gene="era"
                     /locus_tag="RD521_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDT7771662.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFTQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            22606..23418
                     /gene="recO"
                     /locus_tag="RD521_001953"
     CDS             22606..23418
                     /gene="recO"
                     /locus_tag="RD521_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDT7771663.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            23802..24719
                     /gene="glyQ"
                     /locus_tag="RD521_001954"
     CDS             23802..24719
                     /gene="glyQ"
                     /locus_tag="RD521_001954"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDT7771664.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            24721..26796
                     /locus_tag="RD521_001955"
     CDS             24721..26796
                     /locus_tag="RD521_001955"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDT7771665.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGKASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT         8975 a         4743 c         5823 g         7540 t
ORIGIN      
        1 aaactggtgc gggaaaaacg acgatcatca aattactgat gcgtgaatac gatgattatc
       61 aagggaaaat ccaatttggc ggacgttcga tcaaagaata ttcattgaat gctttattag
      121 gatcaatcgg ctatgtgcct caggatcact tcttgttttc gatgaccatt cgagataata
      181 tccgtttttc taatccaaaa ctttcagaag aagaagtaga gcatgcggct tctttagcat
      241 ttatcaacaa tgaaatcaag gaattaccaa aaggttatga taccttagtt ggagaacgtg
      301 gagtctcttt atctggcgga cagaaacaaa ggatttcgat tgctcgagca ttgatcaaaa
      361 atcctgaatt attaatatta gatgatgcac tctctgcagt agatgccaaa acagaagaag
      421 ctatcttgca taatttaaaa gaaataagaa aagagaaaac aactatcatt gcagctcacc
      481 gtttaagtag cgtgatgcat gcaaaagaaa tcattgttgt cgatcaaggg aaaatcattg
      541 aacgaggaac ccatcatgag ctgcttgcac taggcggctg gtatgaacaa atgtgggaga
      601 aacagcaatt agaagcgaag atagaaggag gcgaggcctg atggatgaaa attatcaatc
      661 ggaatggaca aaatcgattc cacttaaaga acagaaaaag atcatcaaac gactgatgaa
      721 gttcgccaaa ccttttagaa gaacgttcat cgttgctatc ttatttgcgt ttgctttgtc
      781 tgtgatcaat gtcctattgc caaggatcat ccaaactttt atggatgatc atctggctaa
      841 acagtcggct acgacacaag ttattttgtt ttttgctggt atgtacttat ttggagtcat
      901 agtaaaaagt atcatttggt ttttccaatg gtatttgtat tccatggcct cattgaagac
      961 ctatcagtat attagggtaa aattatttga aaagctgcat acactaggta tgcgttattt
     1021 tgatcagaca ccggcaggat caaccgtttc tcgagtgacg aatgacacag aaacgctatt
     1081 tgaattttgg tacgtctttt tgatggtatt gactgggatc tttgcagtcg tttcttcatt
     1141 ccttgccatg tttcaaatca atggagaaat tgccttgtgg actttggtgt ttttgccgat
     1201 tttaggaatc gtcatctggt attaccagaa attcagttct cgtatttacc gaaatatgcg
     1261 ggaaaaatta agtcagctga acacgaaatt gaatgaatcg atttccggca tgcgtattat
     1321 ccagcagttt cgccaagaag ctcgtttagc taaagaattt gaacaagtga acaatgagta
     1381 tttagacacg cgttatgcga tgatcaaaac gaattcgatt ctgttaagtc cgatcatcaa
     1441 ttttctatat gcattggcga ttgcgttggt gttgactttg tttgggatcg atgcgttgca
     1501 ttctcccgta gaagttggac tgatctatgc atttacgacc tatgtccagg gattttttaa
     1561 tccaatgtct caaatgatgg actttttgag catcttcaca gatgggatgg ttgctggtag
     1621 tcgtatcttg aagattatgg atacggaaga atatgcacca caacaaaacc ctggagcaga
     1681 tcaaatgatt acttcaggta agattgagtt tcgcaatgta agcttttctt atgatggaga
     1741 gcacaatgta ttaaacaata tctcttttgt tgctaatccg ggagagaccg ttgcattagt
     1801 gggacacacc ggaagcggaa aaagctcgat catcaacgta ttgatgcggt tttacgaatt
     1861 ttatgaaggt cagatattga ttgatgatca tgacattcga gaatatccaa tggaagagtt
     1921 acgtaagaaa atggggctgg tcttgcaaga tgctttcttg ttttatgggg atatcgcagg
     1981 aaatattcgg atgatgaatc cagctatcac agacgaacag atacgccatg cggctgagtt
     2041 tgttcaagca aacaaattca tcgatgaatt accaaaagga tatcactcaa aagtgatcga
     2101 acatggtgcc agctattcta gtggacaacg acagctgatc acttttgcgc gaacaatcgt
     2161 gactgatcct aaaattctag tattggatga agcaacagca aatatcgata ctgaaacaga
     2221 aggattgatc caagaaggat tggcaaaaat gcgtcaagga cggacaacga ttgcgattgc
     2281 ccatcggctg tcaacgattc gagatgcaga attgatttta gttttagaca aaggtcatat
     2341 agtagaacgc gggaatcatg aaacacttct agcgcaaaat ggtttgtatg cagatatgta
     2401 caaactgcaa agcagtggta tataaaagat gttgtgacag tagaattttg acctaagcac
     2461 tagagtgaaa ataaaacata gcttctcata tttttcatct gtgagagtta gtgtcgtagt
     2521 caccagattt aaaaaaagat tcgacagcta gtcagaagtg cctaatagcc actaagaaag
     2581 cgttgacaaa agcatttgct ttcgataata taaagaaaac gatacaactt ggatagtgat
     2641 tgcctgatgc aagctaaacc tgatgagtct cccatggttt catggacggc tttttgggtt
     2701 tttctttttg ttttggatac agccctatac atcatggaaa ggagcaaaac atgctgattg
     2761 agaaagtact gaataacaac gtcgtaataa ccagggatga aagaaatcag gaaatgatcg
     2821 tgatgggaaa aggtttggca ttcaaaaaga aagtgggtga cccgattttt gactcatgca
     2881 tcgacaaaaa atttcagttg acaaatgacg ggctttcaca acagttccaa gaattggcac
     2941 aagcgattcc tcttgagtac ttagaaattt cttgtgaaat cattgaatat acaaaagagg
     3001 ctctacaagt ccagttgaat gattctgtgt atattgcatt gacggatcat ttgtatacgg
     3061 ctatcgaacg tcacaaaatg ggaatatttg taccaaatgt attgctgtat gatatcaaac
     3121 aactatttcc taaagagtat gccatcggga aaaaaacagt gaagaaaatc tcggataaat
     3181 accacatcaa gttatcagaa gatgaagccg gatttatcgc attgcattta gtcaatgcac
     3241 aaggcattca aagccagcaa gacaatacct acctgtttac tataacaata caggatattc
     3301 taaatatcgt ccgctattac tataagatta catttgatga agattcagtc catttttttc
     3361 gattcgtcac acacttacgt ttttttctga cacgcgtcct agatggagaa aaatcagaag
     3421 tgggagaagt ttctgaggaa ttgtttcagg ttattcaaaa aaaatataag gatacgtttg
     3481 gttgcgttga gaaaatagca gactttttag ccgagacgtt caactaccag atgtccacgg
     3541 acgaaaaaat gtatttaacg atccatactg cgcgtttagt gcatgagaat cgttgtaaat
     3601 aaaaaaagga ggataaatta acaagaacgt atgaaataag ggtagtgaca ttcagttggc
     3661 taaaccttcc actaataaat tggagggaat agaaatggga aaataccaag aattagcgag
     3721 aaaaatcgta gaaaatgtcg gcgggaagga aaatatcaat ggtttgacac attgtatcac
     3781 aaggcttcgt ttcaaattaa aaaacgaaga aaaagcaaat gatgagatct tgaaaaatat
     3841 ggacggtatt gtaacggtaa tgcgagcggg agggcagtat caagttgtca ttgggaatca
     3901 tgtgccagtc gtgtttgaag aggttataaa agcaggaaac cttacttttg atgaggctgt
     3961 ttcaacagaa aaaatgagac cgtttgatat gcttattgat ggaatatcgg gctgtttcca
     4021 gccattcctt gctatcctag ctgctggcgg gatgattcgt ggactgacag cattccttgt
     4081 cttcttaggc gcgtttgacc gcggttctgg cacatttgtt atgtttgata atatcggtga
     4141 ttctgtattc caatttatgc ctgttattat cggtttaaca gctgcccgta aatttaaagt
     4201 aaatgaattc gtagggatgt tgcttggcgc agcattaatg aatcctagct tgtcgctgga
     4261 agctttgagt ggtgcagcag aagcaccact aacgaccata ttttctggca ctatttttga
     4321 agcacctatt tatcaaacag tattcggcat tccctggatt gcgcgtaact acgcttcttc
     4381 tgtcattccc attatcttta ttgtgttgtt agctagccaa attcagaaac caattaaaaa
     4441 gttagtacct gagatgatcg cgaatttctt cgttccattc ttcaccgttc ttattacaat
     4501 gcctttaggt tttttactcg ttggaccagt attcacattc gcaactgata ttttaatggc
     4561 tggatttgaa acacttttag cactttcgcc agttatttat ggagcaatcg taggcttctt
     4621 ctggcaaatt ctggtgatgt ttggtctcca ttgggcaatc gtaccaatgg gattgatgca
     4681 attttctgtg aatggttggc aaaatattat gacacctgtt gcagtagtgt cttttggaca
     4741 aactgcagca cttaccgcac tctactttaa gcttagaaat ccaaaagaca aagcaattgc
     4801 aataccagcc attgtttctg gtattgtggg gattacagag ccagctattt atgggtttac
     4861 attaccacgt aaaaaaatat ttatttttac ctgtgtcggt ggtgcgttag ggggcgcgta
     4921 tagtgggtta atgaatttaa cttcttggaa tcaaggtgga ctaggcatct ttacaattcc
     4981 caactatatt agaccagatg gtgatttaac agatgtgatt aatgtattga ttgggatcgc
     5041 cattgcgatg gtggtatcct ttacgctgac ttttttcttt tggaaggatg aagcaggtga
     5101 aacagatgat atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca
     5161 aatcgctcca ctaaatgcag caaaggatgc agcttttgca caaggaacac taggtcgagg
     5221 aatcttgatc taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagtcatgac
     5281 cctgttcccg acgaaacagg caattggaat ggtttctgaa acagggctgg aattgctgat
     5341 tcatgtcgga ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca
     5401 aggataaaga gtgaaacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa
     5461 ggaaggatac agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat
     5521 tttggaaaac caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg
     5581 aacggttagg tgaagaatgg actaggctta actattcttt ttattccact tctttatttt
     5641 caaaattgaa aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac
     5701 cgacagaaat tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata
     5761 acacgtctag aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca
     5821 gtcgctcgcg tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac
     5881 cgatactggt caaagctaaa attggaacat gattttgttt caatatttct gcggtacgaa
     5941 ggatcacatt gttttcaccc gtataagaaa tcagaatccc gcacatatca ctcgtgcaat
     6001 tgtacgcttc atagaatgat tcaccaaaag tggtgacaat cgtgacatcc attttgatgc
     6061 gcttcatttt cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaaaatca
     6121 cgattttttt tgcttgaagc attagttgtt tggctttttg cagttggtca tgatgaatga
     6181 gagaaagtgt atcatgaatc gtcatctgtt ctaaagaagc cattttattt gcgatcgtca
     6241 taataccgtc tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa
     6301 gataggctgt ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt
     6361 tttttgctac acggatgagt gtagaggggt ggacataaca tcgctcagca atcgcttgga
     6421 tggtcatttc gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg
     6481 aaggagaaaa ggagatttct ttgatttttt cacgtaataa catgtagata ccttcttctt
     6541 atttttttac gtaaattata acttttttga aaatgatttg taaacggatt tatactatat
     6601 aacttaaaat agacattttt tgacaagata tttacaaaaa tccactagaa aacagtaaat
     6661 tttgtaaatg ttactggaaa gaaacagatt tctattgttt gttttgttta caaaaaagat
     6721 aatgaaagtg taaaaaagta aaggagtgag aacatggcaa aaggaatcaa aatcgctacg
     6781 attggtggag gcagcagtta cacccccgag ttgatggaag gatttatcaa aagatatgag
     6841 gaattaccga tacgagaaat ctggctcgta gatgtagaag aaggaaaaga aaagcttgag
     6901 atcgttagtc agatgtctca gcgtatgtgg gatgcttctc cgtatgaagt caagatctat
     6961 gcaactctgg atcgggaaga agcattgaaa gatgcggatt ttgttacgac tcagtttcgt
     7021 gtcggtcaat tagatgcacg aatcaaagac gagcggatcc ctagttatta tgggatgatt
     7081 gggcaagaaa caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt tccagtcgtt
     7141 ttggcaatcg ttgaggacat gaagcgttta tgtccaaatg cttggctgat caatttcgcg
     7201 aacccaagtg ggatggtgac agaagcagtt atcagatatg gcaaatggga taaagtgatt
     7261 ggattatgca atgtgccagt gatggcgatg atgacagaac cagcaatgct tggcaagaaa
     7321 aaagaagagc tgatctacaa gtttgccggt ctaaatcatt tccattggca caaagtagca
     7381 gataataaag gaaatgaccg cacggcagaa ttgattgatc gtttatacga tgaagagaat
     7441 ggattaccga aaaatatctt tgatatcccg tttttcaaag aacaattaaa acaaatgaac
     7501 atgatccctt gtggctacca ccgctattac tatcgtgaag aagaaatgct ggaacatgct
     7561 ttagaagaat atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac agaagcagag
     7621 ctatttgaat tatacaaaga tccagaatta gaccataaac ctgaacaatt gcaaaaacga
     7681 ggtggcgctt attattcaga cgctgcctgt gaaacgattg cttcaatcta tggaaataaa
     7741 gaaacacaga tcgtggtatc tacaaagaat gatggagctg ttcctgacct gccggcagat
     7801 tgtgtagtag aagtcactgc ctatgttggc gcacaaggag cgagaaatgt agcgtttggt
     7861 tctcttcctg ctgctgaaag aggctggttg caagtaatga agaatatgga attgctgacg
     7921 atcgaagcag ctgtgactgg tgattatggg acagctttac aggcgtttac gatcaatcca
     7981 cttatacgat caggaaagac agcaaaacgg atcatggatg agttgtttat tgcacaccag
     8041 cagtatttac cgcaattcaa ggagacaatc agccgattaa aagaagaagg aatcgaagtt
     8101 caagatgaac tggcaagaga attatcaggt aatacacata taatggagga agtataatgg
     8161 gattcagaaa agatttttta tggggcggtg ctacagcagc caatcaatgt gagggcggtt
     8221 atgatgaagg tggacgcgga ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt
     8281 ttcctgttat caccggaaag aaaaaaatgt ttgaatttga tgaggatcat ttttatccag
     8341 caaaaattgc aatcgatatg tatcatcgtt ataaagaaga tattgctttg tttgcagaaa
     8401 tggggttcaa gacttatcga atgtccatcg cctggagtcg tatttttcca atgggggatg
     8461 agaaagagcc gaatgaagct ggcttgaaat tttatgagga tgcctttaaa gaatgccaaa
     8521 agcacggaat tgagccactt gtgacgatta cgcattttga ttgtccaatg catttgatca
     8581 aagaatatgg tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag
     8641 tcatcttcaa tcgctataaa ggacttgtca aatattggct gacattcaat gagatcaata
     8701 tgatccttca tgcaccattc atgggcgcag ggctgtattt tgaaaaagga gaaaatgaag
     8761 aacaaatcaa atatcaagcg gcacatcatg agttggttgc aagtgccatc gcaacgaaga
     8821 ttgctcatga agtagatccg gagaacaaag taggctgtat gctggcagca ggagcatatt
     8881 atccatacac ttgcaatcct gaagatgttt gggcttctag aaaatcagat cgagaaaatt
     8941 atttcttcat tgatgtccag tcacgaggag aatatcccgc ttatgcattg aaagaaatgg
     9001 agcgaaatgg cattcagatc gaaatggaac caggggatgc cgcattgctg aaagaacata
     9061 ctgttgattt tatctctttt tcttattatg cttcccgagt ttcgactacc gacaacgaac
     9121 tcttggaaca gactgcagga aatatctttg cttctgtcaa aaatccatat ctgcaagcaa
     9181 gtgaatgggg gtggcaaatt gatccattag gcttgagaat aacgatgaat gatatttatg
     9241 atcgttatca aaaaccattg tttattgtag aaaacggctt aggtgcagtc gatacgccag
     9301 atgaaaatgg ttatgtggaa gatgattacc gaatcgatta cctggcagcg catatccagg
     9361 cgatgaagga tgcagtagaa ctagacggag tcgatctatt aggctatacg acttggggat
     9421 gtattgattt agtatcagct ggtaccggtg agatgaagaa aaggtacgga ttcatttatg
     9481 tcgatcgtga taaccaagga aatggcacat tagcccgttc aaagaaaaaa tcattcaact
     9541 ggtataaaaa agtaattgat tcaaatggag aaaatctttc gaacgattga tcaatgattt
     9601 ataaaagtct attgtttttc agcggatttt tcgagtataa taaagctatt cgagaaacgc
     9661 aagcagaaca atagactttt tttcgcattg gctgtaaagt atgcttactt gattaagtaa
     9721 aggagaatct acatatgtat gatgtaatcg ttattggcgc aggtcctgct gggatgactg
     9781 ctgctttata tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg
     9841 gcggccaaat gaacaatacg gcagaaatcg aaaattatcc tggattcgat tcgatcatgg
     9901 gccccgatct agcttataag atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt
     9961 atggaatcgt tcaagatatc aaggatcacg gcagccataa agaagtcatc tgtgaagata
    10021 aaagttacga aggaaaaaca gtcattatcg caacaggatg tatccaccgt aaattaggcg
    10081 tacctggcga ggaagaattt gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg
    10141 cgtttttccg taacaaacgt ctagtagtca tcgggggtgg ggactcagca gtagaagaag
    10201 ccatctattt gacacaattt gcttctgaag tagtgatcgt ccaccgtaga gatgaattac
    10261 gtgcacaaaa gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg
    10321 atacagtcgt tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg aaagcacgta
    10381 atgtcaaaac cggcaatgaa tttgatttag cagcagacgg agtattcatc tatgttggac
    10441 tggatccatt aactgcacct tttagagaag caggtttgac gaacgaagct ggctggattc
    10501 caacagacaa cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga gatgttcgtg
    10561 aaaaagattt gcgacaaatc actacagctg ttggtgacgg cggtattgct ggacaacaag
    10621 tgtataaata cttggagtct ttagaagaaa caacagcata agtttaaaaa gagtagaaga
    10681 acgggttcaa ttgttctttt actctttttt ttcgcgtgaa atttactgaa tctgatttat
    10741 gcgaacacgt ccaacacctt ttgataaaag aatcctttct tgatttaagc gttttttggt
    10801 cctcatactt tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa
    10861 gaggtgttat tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt
    10921 tagaagaaaa cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag
    10981 atgcttttta tgcaccgttg gaattcggta ctgcaggtat gcggggaatc ttaggacctg
    11041 ggatcaatcg aatgaacatc tatacggtaa gacaagcaac agaaggacta gcacgtttca
    11101 tggatacaca aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc
    11161 atcagtcacc agaatttgca atggaagcag ccaaaacact agcacatcac aatatcccgt
    11221 cttatgtatt cgaaagtctg cgtccaacac cagagctttc ttttgcagtt cgtcatttga
    11281 agacgtttgc gggaatcatg gtaaccgcat ctcacaaccc agcggcttac aatggatata
    11341 aagtctacgg agaagatggc ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat
    11401 atgtccgtga agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac
    11461 atagcgggtt gatcacgatc atcggagaag aagtggacgc tgcttatcta gaagaaatca
    11521 aagcagtgac gattgatcgt gaattggttg aaacaatggg gaaagatttg aaacttgtgt
    11581 acacgccact tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat
    11641 tcgaacaatt cgtactagtt cccgaacaag ctgtggcaga tccagatttc actacggtca
    11701 aatcgccaaa tccagaagaa cattcagctt ttgaatatgc tatacgactt ggagaaaaag
    11761 aaggagctga cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc
    11821 gtttgccaga tggttcttat gaagtattga ctgggaacca aatcggagca ttgtttgctc
    11881 aatatatttt ggaagcacat caatcaagag gcactttacc agaaaacgca gcggtattga
    11941 aatcaatcgt ttccagcgaa ttgcctacag caattgcgga acactacggt gctaccatgt
    12001 tcaatgtgtt gacagggttt aaattcattg ctgaaaaaat ccaacaattt gaagaagacc
    12061 attcacatac ttttatgttt ggattcgaag aaagttatgg ttatttagtt aaaccttttg
    12121 ttcgcgataa agacgcgatc caagcattgc ttttattagc agaagtagca gcttactata
    12181 aaaaacaagg caaaacgttg tatgacggct tgcaaacgat ttttgaaacc tatggctatt
    12241 tcgcagaaaa aacgatttct gtgacaatga gcggacaaga aggttctgcg aaaatctctg
    12301 ctttgatgga aaaattccgt aaagaagcac cgacagaatt tgccggagca aaagttattc
    12361 aaacagaaga ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa
    12421 caacaccgcc atccgacgta ttgaagtata cattggaaga aaatggctgg atcgctatcc
    12481 gcccttcagg aactgagccg aaaatcaagt tttatattgg tgtaaaagga agctctacag
    12541 aagacgcagc cgaaaaagta gcctcttatg aagcgattat caaaaagatg acagaagaat
    12601 aaaaaacttg agactgatgg aaaaagtggt aaactttaga agtcagtctt ttttcaagtt
    12661 ggaggtgaat ggattgccag acacgcaaaa atcaaaagta gagaaagtca gtcaattatt
    12721 caaaatgttg agtgatccca ctcgtcttaa aattctgcta tatctgaaag acggggaaca
    12781 aaatgtgaca gcaatcaccc aagcagtgga aatggagcag tctgctgtat cgcatcaact
    12841 acgtttatta agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta
    12901 tagtcttgat gattctcatg tactggatat tttgaaccag acactcaaac atgtggaaca
    12961 tcaataacga taggtgaaaa tagataaaca ctggagtgtg acaacgaaga aatttcgttg
    13021 tcacactcca gtgtttttta agaaaaagac aaaaatgatt aatcgctttt ttctgttgtt
    13081 tcagttccat aataactatg atcttctagg tctaaagcat tcaaaataag cgaagccgtt
    13141 tcttcaatag ataaagatgc cacattgatc actacacatc ctagtttttc ataaagatca
    13201 ttcgcaaaat cgagttcagc tctaattttt tcgatatcgg aataagcagt atcagggttt
    13261 aatccgtaag agcgcatccg ttctttacgg atattattta aaatatctgg gttattcgtt
    13321 agtcccacga tttttttagg atcgatttcc cacagctgtt tgggaatatg tgcttgggga
    13381 atcaaaggta aattagcaac ttttaaattt ttatttgcta aaaacaagct caaaggcgtt
    13441 ttggaagttc gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc acgaggatct
    13501 ttcccatcat cgtattttac ggcaaattcc attgctttga tccgtttgaa atagttctca
    13561 ttcaataaat ggagggcacc aggttgccga attggcttga taccagttaa acgttcaatt
    13621 tctgatatcg gtggcgtcat cacatcgaaa ctaaataatt gatgttcttc acaaaaagaa
    13681 cgtgttatct cgatcagttc ttgattgatt aatgtatgca ggacaagtgc tttttcttct
    13741 aatgcatctt gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt tctgcggaat
    13801 aggttgaact caacggtagg atactgagcc atagtagctt gcgctagttt tgtcgcagtt
    13861 tctccagcag agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg
    13921 gataaaacct cacttgcatt ttttttatgt ttattatacc atactctaga atgcgtactt
    13981 cgtaatcatt ttgatttgga ggaataagtc atgaaaaaac agacattgat cgaacaatca
    14041 ttagaaacat tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact
    14101 tatttagtga agcgtaaccg ctacgtatct gctaaagaag tttttgacta tatgaatcag
    14161 gaattcaaag gggtaagtta tgatacgatt taccggaatc tccatgattt tgcagagatc
    14221 gatttactgg aagaaaccga tttaaatggt gaaatgaagt ttcgttttca ttgctgtcag
    14281 ggggaaatcg gccatcacca tcattttatc tgtacagtgt gcggaacaac gaaagaaatc
    14341 catatgtgtc caatgaatta tttcgaagaa cagctaggtg gatgcacgat cgaagggcat
    14401 agattcgaga ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt
    14461 tgataattta aaaaaaaaag caagatttcc ttgacggtca aaacagcatt agatataata
    14521 tttacaggac agtcttgttt tttgagatat atttcaataa caattacggt tactttttaa
    14581 gctcggaggg agggaattaa catgtcaaaa acagtcgttc gcaaaaacga atcacttgac
    14641 gatgctcttc gtcgcttcaa acgttccgtg tcaaaagcgg gtactttaca agaatctcgt
    14701 aaacgtgaat tctacgaaaa accaagtgta agacgcaaga aaaaatctga agcagctaga
    14761 aaacgcaaaa aattctagtt ctgtgatgaa ttggagttgg tagtgtgtca ctactaagta
    14821 gattaaacga tgacatgaaa gcagcgatga aagcgaaaga taaggaatct ttacaagtca
    14881 tccgaatgat caagtcatct atacaaaacg agcagattaa agaaggtcac gacttgacag
    14941 aagaagaaga actaacggtg ttgtctcgcg aaatgaaaca gcgacgtgat tctttgcatg
    15001 agtttgaaga agcaggacgt gacgatttag ctgaaaaagt aaaaagtgaa attgtaatag
    15061 tcgaaaaata tatgccggaa caactttccg acgaagaaat tcgacaactt gttcaagaag
    15121 caatcactca aactggtgct tcatcaatga aagaatttgg caaagttatg ggtgcaatta
    15181 tgcctaaagt caaaggaaaa gcagatggaa accaagtgaa tgcgattgtg aaagaactat
    15241 tacaatctta attttatcat ttaaactgcg gacagtgatt gtccgcagtt ttttgtgttc
    15301 gtaaaaaaag aactaaaaaa cagctagaag aaagaatctt attctttctt ctagctgttt
    15361 ttttcctgtt taatatctct tatgttaaat tgtgtttgat ttggttattc ttctggtagt
    15421 tcatttgctt cttgtttata ccctgatagg cgataggcgt aataaaagag taagctgcca
    15481 gcgtaaccaa gtaacgttgg gaccatccag ccaagtccta atgcagcaaa aggaacgtga
    15541 tgatgataaa atcctagtaa gcttgctacg acagtttgct gactcactaa tgcaggcgaa
    15601 tttgccaaca tatccaatac ggctggaacg gcagaaaaga acatcgttac ttgatagaca
    15661 aatggactct tttggaagaa ttttgctgtt aaggaaagca agattaacgc aagtgacaat
    15721 ggatacaaaa gcatcaatac tggtaaagac cattggatga tattatccaa tccggcattc
    15781 gcaacgacaa aagagacaaa agtcgttaac cgcagccatg tcatataact gactttaggg
    15841 aacagtttat ggaaatcttg tgcaaaagaa acaacgagtc ccatagcggt agtgaagaca
    15901 cctaatgtga ccattacccc taagaagcta gcaccgaagt tacctaaata aaaattagtg
    15961 atctgagaaa gtgcagttcc gccattttct gataaagaca tcttattcaa actcatcgca
    16021 ccgagaagga ccagaccaaa ataaatcatg acttctaacg caacgctcag gctaccagct
    16081 tttgctgtta attctgtgac ttttttatct ttaaaaccaa gatcacgaac tgcatggaca
    16141 aaagtgacac ttaaagcaag taaagctact gcatcaaccg tgttgtatcc ttctaaaacg
    16201 ccatttgtca aagcgttcgt ctgataagaa tctgcagcag attggtttag attccccatg
    16261 ggttggaata aagaaacgac aaaaatgata gctaataatg caagaaacaa ggagtttaaa
    16321 tactttccga catatttgac caaacgtgtt ggatgtacag taaggaaata agcaagtaaa
    16381 aagaaaagag cagagaataa aaacatacct aatgaatcat atttttcagg caaaaagggt
    16441 ttagcagcca tttcaaaagc agttgctgct gtccgcggtg taccaaagaa tggtccgatc
    16501 gttaaatgaa caaggatcaa gaacaaggca gcataccatt ttccgacagg tttagctaag
    16561 tcatacagac catcactttt tgtcactact acagctaaga tagctaataa tgggaaaaga
    16621 gcgcctgaga tggcaaatcc tacaccagcg ctgatccagt ttgatccagc catttgacct
    16681 aagtggacag gaaagatcag attacctgac ccgaagaaca tcccgaagat aagtgaactg
    16741 accatcaata attgcttcca tgaataagtt tttgtttctt ccataaattt ttcctccaat
    16801 aattatcatt ttgagtaaca aaataaacgt tgcagaaact agaaatgcca ctaatatcag
    16861 attccatgaa aaccaccacc ttaaaatata aaaaaaagcc cttttccact tgggaaaagg
    16921 gctctagatt ctagaacggt accacctttt ttcgcttaaa aaagcttctt cattcacgga
    16981 cgcttttcga taaagttatc cgctagcctg ataatggagg cagccacaat gatctacgtt
    17041 ttcgacattg actcggaaat gatgttcgtc tattcgattt attgtcattt ctcacttaca
    17101 tgactcactg ggaataaatc gctgaatagc ctactgtttt cctcgacgct ttgctgtata
    17161 tttatttgtt gacgttataa tacacctgtt ttttttgaaa tgcaagaata tttcaaatat
    17221 tttgttttct ttttttgaaa caagaaaaat acccagagaa tacttgctaa gttagacgga
    17281 atacttgtcc tctatcttat gcatagctta catgatcagc tttttttatt caaacgagac
    17341 ctttagagaa tggaagcagc tattttttaa cgtttcttgg tacttatggt taagcaactc
    17401 ttttagaacc ttttcttatt tgctatatag tttcttctga gtatggtaaa ataggaaaga
    17461 atgaatcgaa aaaaagggga tgtctatttt tgacggaaga gtctagttct ttagagatta
    17521 gattgacggc tgctgatgat gtcagcatgc ttttaggtgc acacgacaag catataaaac
    17581 tgattgaaga aacaacagaa acaaccatcc atacacgtgg agaaatcatt cagatcattg
    17641 gtgaacaatc ccagatttcc ttggctgcct cagtcattcg gacattgcag gaattgatca
    17701 aacgaggaat ccatgtaagt acaccagatg tcgtgacggc actgaaaatg gggcaaaaag
    17761 gcacattaga ttattttgtt gagatgtatg aggaagagat cgtcaaagac cgtaatggaa
    17821 aacctatacg ggtaaaaaac agcggccaaa agaaatatgt cgaatcggta gccaaacatg
    17881 atatcgtatt tggcgtagga ccagccggga caggaaaaac ttttcttgca gtagtattag
    17941 caatcgctgc cttgaaaaaa ggtgaagtgc aaaaaatcat tttgacgcgg cctgctgtgg
    18001 aagcagggga aaatctaggc tttcttcccg gggatctaaa agaaaaagta gatccttatc
    18061 tgcgtcctgt ttatgatgct ttataccaaa tttttggatt agatcatacg aaccgattga
    18121 tggaacgagg cgtgatcgaa attgctcctt tagcttacat gcgggggcgg acattggatg
    18181 atgcattcgt tattttggat gaagcacaga acacgacagt tgctcagatg aaaatgttcc
    18241 tgacacgtct tggttatcaa tcaaaaatga ttgtcaatgg agatacgagt cagattgatc
    18301 tgcctcgagg gacaacaagt ggtcttgttc atgcagaacg aacattaaag caaatcaaga
    18361 aaatcgattt tgttaatttt gaagcaagtg acgttgttcg gcaccctgta gttgccgaaa
    18421 tcatcaaagc ttatgaaaag gcagatctcc atcaagaata agctttgctt aggaggataa
    18481 gcacatgttg aataaaacac tgcagaaact acaagaaaaa cttggaaagg cagcattgcc
    18541 tttcctgctg ttcttgtttt ttatcattat ctgcggcgtt acatttagta gtgtcaaaca
    18601 aaaggccaat gatttcagag aaggccaagt agcagaagaa agtatccgtg caaacaaaac
    18661 gatcgagaac acggaagaaa ccgaacaaaa aagaaaactg gcagcagaag cagtcactcc
    18721 agaatacact taccaaaaag atcttgcaga tgaccagaat aatcggatca agcaactatt
    18781 tgaattgatc gataagacga atgatgccat caacaaatct tatgacgaaa agattgataa
    18841 ggcaaaggaa gatgaaaata tcccaaagcc tactacagaa gagcggatcg ccgcgttgaa
    18901 gaaaaatttt gaaaatgtga atgcagaaaa tgttgcgttt tatcaaaaac taccgaacaa
    18961 tttttattcg acgatatttg aaatgactga aactgagatc catactgtga gggatgagag
    19021 tctgaagctc attgacgaac agatgagcaa acaagttcgt gaaagtgaat tggaagcttt
    19081 taaacaagaa gcagaagagc aaatccaata tttgaatgtc acaccagcac aacaacaaat
    19141 gattcgctac ttagtggatc aaggtatcgt agtgaatgac gtattgaatg agaaaaagac
    19201 agaagaattg aaacaatccg cacgagaagc tgtacaacca gtgatgatct tccaaggaga
    19261 aatcattgtt cgtgaaggta atcaaatcga tgcttcagct atgaaaaaat tggaattact
    19321 aggactgact aatcaaacaa cttcgatttt tccgctaatt gccatggttt tagctgtttt
    19381 gcttcaaatc ggcgtactga tttataattc acttcaattc caaaatgcag gagaaagaac
    19441 aaaatacgtg cttttctatg tgacagctat gtcagtcagc atagtgctga tgaaattttt
    19501 ccaattattc caaacggaac aagctactta tatcccgctg ttttatcccg ctgcttttgc
    19561 accacttgtt ttaagctttt tcttgaatcg aagagcaggg atcatggctg gtattttcca
    19621 agcagtatct gcacttttca ttttttatga ttcgattggc acgaactttc taacgattat
    19681 cttgatgtct tatgttttct ctgggctcat ggcaacgatt gtcagacgta aacggatctc
    19741 agaacaagga ttatctgctg ccatgtgggt cattattttt cctgtgatga tggatgtcat
    19801 tttgatcgtc tatcaaggaa tgagctttgg tgatgcaacg acttggttaa tgctcgtctg
    19861 cgggttcact ggtgcactct tctcattctt gctgacgatc ggtctacacc catatattga
    19921 attgatggtc aatgatgaca gtgtaattgt attaaatgaa ctcagcaatc caaatcatcc
    19981 tttattgaaa cagttgctgg aagaagcgcc aggtacttat catcatagta tgatggtagc
    20041 taatctaagt gctaacgctg tagcagaaat cggcggacgt tctttgctaa ctcgagtagc
    20101 ttgctattat catgatatcg gcaagatcaa acatgctagc tttttcgttg agaacttgcc
    20161 ttcaggagcg gaaaacccgc acaatttctt attgccagaa gacagtaaac atattatctt
    20221 tggacatgtg acagatggag ccaaaatatt ggaagattat aatatgccac aaatggtgat
    20281 cgatatttgc agacagcatc atggaaccac gctaatgcgt tatttctaca tcaaagcgaa
    20341 agaacgcaat ccggaagtaa cagaagagca attccgttac cctggaccaa aaccgcaaag
    20401 tcgtgaagca gggatcgtca atattgctga cagttgtgag gctgcagtac gggcaatgga
    20461 tcatccgacg agcgaaaaaa tcgaaagttt tgtccataat cttatcgaag agcgtatttc
    20521 cgatggtcaa ttagacgata gtggactgac cttgaaagaa atccgaaaag tggaaaaatc
    20581 attgatcagc ggtttaagta gcaccttcca ttctcggatc aaatatccta aaatgaaaac
    20641 agaagttgag aagataaagg aagaacaaga agaatagaaa gaagcgatgt ataatggata
    20701 ttacctttat ggatgaaaca aaccaagtct cagaagagaa aatgaaagaa atcgatgact
    20761 tgctgcaatt tgcagcatct tatttaaagc tgccagagga aacagaaatg tctgttactt
    20821 tcatggacaa tgcggcaatc caagtaatca atcgtgatta ccgtggaaaa gatgcaccga
    20881 cagacgtcat cagttttgct ctggaagaag aaggcgaaga cgaaatccca gtgatttttg
    20941 aagatggaga gaatccattg cctcgtgtac taggagacat catgatctct attgaacgtg
    21001 ctgctgaaca agcagaagct tacggccatt catatgatcg ggaacttggc tttttagcgg
    21061 tacatggatt cttgcatatc aacgggtatg accatatgac accagaagat gagaaagaaa
    21121 tgtttggttt acagaaagag atcttggatg cctatggact caaaagataa aaacgtagag
    21181 aaaaacaaac atttcctgat gtctgtggaa tttgctgtcc aaggagtcaa aaccgttttt
    21241 gaggaagaac gcaatatgag gaaacatgtt gcgttcggta tccttgcttt atttatgggc
    21301 gtgattttcc agcttgatcg gatagagtgg ttatggctgc ttttagctgt ttttctcgtt
    21361 tggatagtag agatcatcaa tactgttttt gaaaatgtag tggacatgtt tacggatttt
    21421 cattttcatc cgattgggaa aaaaatcaaa gatatggcag ctggagcagt tttagtgacc
    21481 gcttgttttg ctgtcatcat tgggttgatt ttatttgtcc ctaaaatata tcatttgttg
    21541 tttaattaaa aaggagaacg tatatgccag aacataaatc agggtttgtc gcaatcgtcg
    21601 gccgcccgaa tgtaggaaaa tccacattat tgaaccgaat cgtgggtcaa aaaattgcca
    21661 ttatgagtga caaggcacag acaactagaa ataaaatcca aggagtatat actacaccag
    21721 aagctcagct gatttttatc gatacaccag gaatccataa accaaagcat cgtctaggag
    21781 attttatggt agaagcagct tacagtgcgt tgaaagaagt ggatgcagtc ttatttatga
    21841 tcagtgctga tcaaaaacgc ggtcggggag acgattttat tattgaacgt ctaaaaaacg
    21901 tgcattctcc agtttatctg attatcaata aaatcgataa gatccatcca gacgagttgc
    21961 tgggaatcat cgaagattat tcgacgcaga tgccttttac acaagtgatt ccgatctctg
    22021 ctacagaagg aaacaatgtc gaacgcttaa tggatgtatt agtcagtgaa atgccagagg
    22081 gcccacaata ttttccagat gatcaagtga ctgatcatcc tgaatacttc atcgtttctg
    22141 aattgatccg tgagaaagtc ttgtttttaa cacgagacga agtgcctcat tctgtcgcag
    22201 tcgttgttga ttcgatgaag cgaaacgaga atgataaaat ccatatccag gccacgatca
    22261 tcgtcgaaag agatagccaa aaaggaatca ttatcggcaa aggcggaaaa atgctgaagc
    22321 aagtaggaac gaaagcaaga aaggatatcg aaaatctttt aggagacaaa gtatttctag
    22381 aactttgggt caaagtccaa aaagactggc gagacaaaaa agtctatctg caagacttcg
    22441 gctaccgaaa agatgaatac tgatattctg aacttcttat aggttaagta tagaaataaa
    22501 ctggataaaa atccgcacgt tgagaaaaac ggcggaagat cgaaacataa ataatctgga
    22561 tcggcagaat ctgcttttcc agatttattt tttaggaggg agaatatgag ccagcttgca
    22621 gaatcgaaag gcttgatttt atctagccgt aattacaaag aaaaagataa attagtcaaa
    22681 atctttacgg aatcttctgg caaaatgatg ttttatatca aaggaatcca tcggcaaaat
    22741 cagccattag ctccggcttt gctccccttt accgaagcag tctatatcgg taattttcga
    22801 agtgaaggtt tgtcgttttt aagcagtgtc aaagacgtcc atgcgtttcg gaacatccaa
    22861 gaagatatct ttttgagcgg ttatggtacc tatttgctaa atctagtaga tgcagcaatc
    22921 gaagatcatc aatatgaccc gcatttattc caatttacgc aacaagctct gcaacggatg
    22981 gatcaaggag atgatgcaga aatcatcacg aatatctttg aagttcaaat actgcaacgc
    23041 ttcggaatcg cgccaatttg gacgcactgt gtcgtatgcg gggaaacaaa aggaaaattt
    23101 gactattctt ccaaatatgg cggtgtgata tgcgagaaac actggccaat ggacgaacac
    23161 cgttatcatg cagatcctcg cgctgtttat ttcgttcgaa tgttttcggc tatttcttat
    23221 gataagatca gtggaatcca cttgaaagaa gaaacgaagc aggcaatccg tgacctcatt
    23281 gaccagttgt atgatgaata tgtcggtatt catctaaaaa gcaaaaagtt cattgatcaa
    23341 atgaagtctt gggcacacgt attaaagacg ccagagaaaa acatgcctga aaaagagata
    23401 cctgaaaaag aagaataaat gaaagataca acaaaaaaat caaaactatt tgacaaatac
    23461 agggacttcc gtctattatt aaccgtagat taaatagatt gttcactgag aggatctatt
    23521 atacaaacaa gaaaactgca aaatgaatac gtacattgac caagatcagt aaaaaacacg
    23581 tggtttagcg agttcgggag agtggaagcc ggacccaaac gatttttgaa gaggcacttg
    23641 cgagtacgat aaatgaagct gccgaatctt tattcggaag tagggtggaa ccgcgaaatt
    23701 attcgtccct atgtctgtaa tcagacatag ggactttttg tcgttttctt tcctgtattc
    23761 ttattgacag caacgcttaa aaattgaaag gggcaaaaca gatgagtaaa aaattgaccg
    23821 tccaagatat gattttaaca ttacaaaaat tttggtcatc aaacggatgc atgctgatgc
    23881 aagcttatga tacagaaaaa ggagcaggta cgatgagccc atatacattc ttacgggcaa
    23941 tcggaccgga accatggaat gctgcttatg tagagccatc ccgccgacca gctgacgggc
    24001 gttatggaga aaacccaaac cgtttgtatc agcatcatca atttcaagtc gtaatgaaac
    24061 cttcacctga aaatatccaa gagctttatt tagaaagctt gcgtctttta ggaattgatc
    24121 cattagaaca cgatattcgt tttgttgaag acaactggga gaacccatcg atggggtgtg
    24181 ccggtgtggg ttgggaagta tggcttgatg ggatggagat cacacaattc acgtacttcc
    24241 agcaagtcgg aggactacct tgtaaaccag ttacttctga gatcacttat ggactagaac
    24301 gtcttgcttc atatattcaa gaagttgaaa gtgtctatga tctggaatgg gctgatggcg
    24361 tgaaatatgg cgagatcttc aaacaaccag aatatgaaca ttccaaatat tcttttgaag
    24421 taagcgacca agacttgcta ctaagtaatt ttgaccgttt tgaaaaagaa gctaaacgct
    24481 gtatcgatga aaatcttgtt catccagcgt atgattatat tttgaaatgc agccatacat
    24541 tcaacttatt agatgcccga ggtgcagtat ccgtaacaga acgagcaggt tatctttccc
    24601 gtatccgcaa catggctcgt gctgtagcca aaatctttgt ggcagaacgc gaaaaattag
    24661 gattcccttt attgaataaa aacacagcaa atccagaaaa catgaaggag gctgaataag
    24721 atggcaaaag acttattact tgaaatcggg ctggaagaaa tgcctgctca tgttgtcaca
    24781 ccaagcatga aacaattaga agaaaaaaca gcaaaatttt tagatgaaaa ccaattgact
    24841 tatgatacga tcgaaacatt ttccacaccg cgccgtttag caatcaaagt aacaaatatt
    24901 ccagaacgac aagcggacag cgaagaagaa gtcaaaggac cagccaaaaa aattgctttg
    24961 gacgcagatg gaaactggac gaaagcagct gaaggatttg tccgaggaca aggtctgacg
    25021 acagatgcta tcacttttag agaattaaaa ggcgtggaat atgtctatgt aacgaaatac
    25081 actcacggca aagaagcaaa agaagtattg acaggtttag cagatgtcat taaaagcctc
    25141 actttcccag taacgatgca ttgggcagaa tacgattttg aatatatccg ccctattcac
    25201 tggatcgtgg cattgttaga tgacgaagta atcccattcg aaatcttgga tgtcaaaaca
    25261 ggcaaagcct ctcgaggtca tcgtttctta ggcgaagatg taacatttac gcatgcaaaa
    25321 gaatacgaag aaaaattaaa tgaacaattt gttattgctt caccgcaaaa aagaaaagaa
    25381 atgatcatca cacaagcaaa cgaattagca gaaaaaaatc actggatcat tgatctagat
    25441 gaagatttac tagaagaagt caacaatcta gtcgaatatc caactgtctt ttctggcgac
    25501 ttcgaagaaa aatatctatc tgttcctgaa gaagtactag tcacttctat gaaagaacat
    25561 caacgttatt tcgaagtacg gaacgatcaa ggtatgttgc ttcctcattt tatttctgct
    25621 cgtaatggcg atcgggtaaa attggaaaat gtggtacgag gcaatcaaaa agtattgact
    25681 gctcgtttag aagatgcgga attcttttat aatgaagaca agaaagcttc aatcgaccaa
    25741 tacgttgaaa aattaaaaac tgttactttc catgaaaaaa tcggtagcat ctatgagaaa
    25801 atgcaacggg tacaagtcat cgctcgcatc ataggcgaac aagtaggact atctgaggaa
    25861 gaattgatgg atctgcaacg cgctgctgaa atctataaat ttgatctagt aaccaacatg
    25921 gtaggagaat tccctgaact acaagggatc atgggagaaa aatatgctct gcttaaaggc
    25981 gagaaaccag ccgtagcaac agcgatccgt gaacattaca tgcctatatc cagtgaagga
    26041 gatctacctg agactacagt aggttctgtt ttagcgattg ctgataaatt agatagtatc
    26101 ttttctttct ttgcagttgg gatgatccca agtggctcaa acgatccata tgctttgcgc
    26161 cgtcaagcat atggtgtcgt tcgaatcatc gaatcgaaaa aatgggcctt tcctttgtct
    26221 gttctgcaag aaacgattag tgaagttatc agtaaagata cggatcgttt tggcattggc
    26281 ttgtcagcgg gacaacaaca ggtgatcgac tttatcaaag gccgtctgcg ccaattattg
    26341 acaactaaga atattcgtca tgatgtcatc gaagctgttt tgaacgcaga acaaaaagat
    26401 ctgacaaaag tatttgcagc agcacaattg ttcaagcagc atcttgcaga tgaagacttc
    26461 aaaccatcga tggaagcttt gacacgtgtc gtgaacttag cgaaaaaagc ggagcttgaa
    26521 aaacaatcag aagtagaccc tgaactgttc gaaaacgaag cggagaaaga actgcacaaa
    26581 gcagtagagg caatgaaaac aagctttgct gaccaaacga tcaacgaaaa ttatgatcag
    26641 cttgtaagcc ttcgcccact gatcgaaaat tattttgatc agacgatggt catggcagaa
    26701 gaaccagcag tacgtaagaa ccgattaacg caattaagtc aaattgctgc catggctttg
    26761 tcactagcaa gtttagatca aatcatcaca aaataatttc taatatgtaa ataaaccaga
    26821 gaacaggcac aaaataggct atttctctgg tttatttgtt ataaatataa ataactatta
    26881 ttaattcaat gttaattttt tttgttattt tattttttgt gtaactttta aaaacaccta
    26941 tgaaaattaa ggaaataaaa atgaaatcga ttaattgtta tcttattaat gttttattta
    27001 tttttaaatc gtaaattgcc ctagtcaaga aaacagtttg tggtatgatt atatagggaa
    27061 aacttgttta caaatatttc t
//
LOCUS       DAPBCS010000041        26054 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPBCS010000041 DAPBCS010000000
VERSION     DAPBCS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 26054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.041"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            289..606
                     /locus_tag="RD521_001956"
     CDS             289..606
                     /locus_tag="RD521_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDT7771666.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="RD521_001957"
     CDS             complement(697..1062)
                     /locus_tag="RD521_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771667.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="RD521_001958"
     CDS             1270..1752
                     /locus_tag="RD521_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771668.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="RD521_001959"
     CDS             1949..2839
                     /locus_tag="RD521_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDT7771669.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="RD521_001960"
     CDS             3165..5639
                     /locus_tag="RD521_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDT7771670.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="RD521_001961"
     CDS             complement(5742..7478)
                     /locus_tag="RD521_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDT7771671.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="RD521_001962"
     CDS             complement(7475..8179)
                     /locus_tag="RD521_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771672.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="RD521_001963"
     CDS             8310..8813
                     /locus_tag="RD521_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDT7771673.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="RD521_001964"
     CDS             8773..9111
                     /locus_tag="RD521_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDT7771674.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="RD521_001965"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="RD521_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDT7771675.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="RD521_001966"
     CDS             10662..11240
                     /locus_tag="RD521_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDT7771676.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="RD521_001967"
     CDS             11244..11765
                     /locus_tag="RD521_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7771677.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="RD521_001968"
     CDS             complement(11844..12398)
                     /locus_tag="RD521_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7771678.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="RD521_001969"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="RD521_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDT7771679.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="RD521_001970"
     CDS             12938..13189
                     /locus_tag="RD521_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDT7771680.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="RD521_001971"
     CDS             13313..14104
                     /locus_tag="RD521_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771681.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="RD521_001972"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="RD521_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDT7771682.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="RD521_001973"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="RD521_001973"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDT7771683.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="RD521_001974"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="RD521_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDT7771684.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="RD521_001975"
     CDS             16339..16581
                     /locus_tag="RD521_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDT7771685.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(16669..16782)
                     /locus_tag="RD521_001976"
     CDS             complement(16669..16782)
                     /locus_tag="RD521_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HDT7771686.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            16932..17186
                     /locus_tag="RD521_001977"
     CDS             16932..17186
                     /locus_tag="RD521_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HDT7771687.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="RD521_001978"
     CDS             17215..18474
                     /locus_tag="RD521_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDT7771688.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="RD521_001979"
     CDS             18527..19423
                     /locus_tag="RD521_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7771689.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="RD521_001980"
     CDS             19434..20303
                     /locus_tag="RD521_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771690.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="RD521_001981"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="RD521_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="RD521_001982"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="RD521_001982"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDT7771691.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="RD521_001983"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="RD521_001983"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDT7771692.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="RD521_001984"
     CDS             23711..24208
                     /locus_tag="RD521_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDT7771693.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            24393..24653
                     /locus_tag="RD521_001985"
     CDS             24393..24653
                     /locus_tag="RD521_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771694.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(25015..25254)
                     /locus_tag="RD521_001986"
     CDS             complement(25015..25254)
                     /locus_tag="RD521_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771695.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25540..25854
                     /locus_tag="RD521_001987"
     CDS             25540..25854
                     /locus_tag="RD521_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771696.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8656 a         4383 c         5314 g         7701 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt
    24301 ttataaaatc acaagtgtta ttgattttca taatcatatt ttaatgttat tatcatttta
    24361 aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa
    24421 aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa gtaatcagta
    24481 aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa
    24541 tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa
    24601 cagaatatct atataactat agcaacaaat atgaacatac tttagtttca taaagattta
    24661 aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca
    24721 gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa
    24781 cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga
    24841 tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac
    24901 tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt
    24961 agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt
    25021 cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga
    25081 ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt
    25141 tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc
    25201 acaagcagta agtaacaata aatttgataa actgataaac agaattcttt tcattatgta
    25261 cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc
    25321 aaaactatca agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg
    25381 atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa
    25441 cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa
    25501 tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta cagtaatttt
    25561 atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa gagtagaaat
    25621 gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg ggtatattca
    25681 cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac actttattct
    25741 tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat ccttatttct
    25801 gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat
    25861 tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg tgaagcctgc
    25921 tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta ggatgaatta
    25981 atttctctag agtttataat tcgccgtttg tataattaag cgagacaaat aaaaaagcca
    26041 tttatgttac actc
//
LOCUS       DAPBCS010000042        25809 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPBCS010000042 DAPBCS010000000
VERSION     DAPBCS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.042"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            103..1554
                     /locus_tag="RD521_001988"
     CDS             103..1554
                     /locus_tag="RD521_001988"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDT7771697.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            1575..2411
                     /locus_tag="RD521_001989"
     CDS             1575..2411
                     /locus_tag="RD521_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDT7771698.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            2411..3070
                     /locus_tag="RD521_001990"
     CDS             2411..3070
                     /locus_tag="RD521_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7771699.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            3196..3885
                     /locus_tag="RD521_001991"
     CDS             3196..3885
                     /locus_tag="RD521_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771700.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            3891..4847
                     /locus_tag="RD521_001992"
     CDS             3891..4847
                     /locus_tag="RD521_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDT7771701.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            4891..5403
                     /locus_tag="RD521_001993"
     CDS             4891..5403
                     /locus_tag="RD521_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771702.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(5659..6207)
                     /locus_tag="RD521_001994"
     CDS             complement(5659..6207)
                     /locus_tag="RD521_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7771703.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(6296..7477)
                     /locus_tag="RD521_001995"
     CDS             complement(6296..7477)
                     /locus_tag="RD521_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDT7771704.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(7713..9221)
                     /locus_tag="RD521_001996"
     CDS             complement(7713..9221)
                     /locus_tag="RD521_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDT7771705.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            9380..9571
                     /locus_tag="RD521_001997"
     CDS             9380..9571
                     /locus_tag="RD521_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771706.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            9774..10691
                     /locus_tag="RD521_001998"
     CDS             9774..10691
                     /locus_tag="RD521_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDT7771707.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            10684..11757
                     /locus_tag="RD521_001999"
     CDS             10684..11757
                     /locus_tag="RD521_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771708.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            11905..12423
                     /locus_tag="RD521_002000"
     CDS             11905..12423
                     /locus_tag="RD521_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771709.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            12404..12796
                     /locus_tag="RD521_002001"
     CDS             12404..12796
                     /locus_tag="RD521_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDT7771710.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            12872..13807
                     /locus_tag="RD521_002002"
     CDS             12872..13807
                     /locus_tag="RD521_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDT7771711.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            13940..14719
                     /locus_tag="RD521_002003"
     CDS             13940..14719
                     /locus_tag="RD521_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDT7771712.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            14712..15029
                     /locus_tag="RD521_002004"
     CDS             14712..15029
                     /locus_tag="RD521_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDT7771713.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            15273..16883
                     /locus_tag="RD521_002005"
     CDS             15273..16883
                     /locus_tag="RD521_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7771714.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            17040..17888
                     /locus_tag="RD521_002006"
     CDS             17040..17888
                     /locus_tag="RD521_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7771715.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            18119..18598
                     /locus_tag="RD521_002007"
     CDS             18119..18598
                     /locus_tag="RD521_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771716.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            18631..20859
                     /locus_tag="RD521_002008"
     CDS             18631..20859
                     /locus_tag="RD521_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7771717.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            20988..22631
                     /locus_tag="RD521_002009"
     CDS             20988..22631
                     /locus_tag="RD521_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDT7771718.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(22704..23249)
                     /locus_tag="RD521_002010"
     CDS             complement(22704..23249)
                     /locus_tag="RD521_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDT7771719.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(23272..23757)
                     /locus_tag="RD521_002011"
     CDS             complement(23272..23757)
                     /locus_tag="RD521_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7771720.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(23747..24559)
                     /locus_tag="RD521_002012"
     CDS             complement(23747..24559)
                     /locus_tag="RD521_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDT7771721.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(24698..>25809)
                     /locus_tag="RD521_002013"
     CDS             complement(24698..>25809)
                     /locus_tag="RD521_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDT7771722.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         8430 a         4718 c         5150 g         7511 t
ORIGIN      
        1 tttttttata aaaaacactc gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag
       61 acgaacttgt gggaaaataa cagaggcaaa aaggaggata atatgaatga tgcaacagaa
      121 ttagcaaaag agcttaaaga aaaagaattt agttttcaag aactagttac ggatatacag
      181 aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg tcgcgcttga accattcgag
      241 agtaattccc acgagctatt gattccccag tcagaaaaag ggccattggc tggtatccca
      301 tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc tagctacttc aggatctcgt
      361 ttatttgaaa cacaccgagc ttctcataca tctaattatg tgcggcaagc agaagcgata
      421 ggtctagttc cttttggaca aaccaatgcg ccagagtttg gtttcaaaaa tattacagac
      481 ccagtcatct atggcccagc aagaaaccct tggaaccttg aatgtacacc gggcggatca
      541 agtggaggag ctgctgctgc agtttcatca ggaatcgttc ctttagctgg tgctagtgat
      601 ggcggtggtt ctattagaat acccgcttcg ttttgcggat tgattgggct gaaaccttcg
      661 cgagggacga tgccagttgg tccatatgct tggagaggat ggcaaggagc tgctattgat
      721 ttcggcttga cagtttctat gagagacacg gaggctttat tttatgggat gagaagtatc
      781 cattccggtg cgccatatca agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa
      841 cggttgaaga tagctttttg tgttgattca ccgatccact cacagattag tgaagaggca
      901 gtacttgctg taaaaaaagc tgtagatttc cttggaaaag aagggcatga tttgacagag
      961 attccttacc cactagatgg tcgtaaactg atcacgtctt attatcagat gaatggcgca
     1021 gaaacagcag caatgatccg ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa
     1081 gaacttgaac ccatttcttg gactttgttc caatatggtc agaagctctc tgccgctact
     1141 tacatcgatt ctctgcatgt atgggatgaa gcagcaattg tcatggaaaa cctacatcaa
     1201 gaatacgatc tattcctttc accaacgact gcatttaccg cccctaaaat cagcgaagat
     1261 ttgcaaagtg atgcgataag agcacagatg gaacacatca gtgattgttc tgaagtagaa
     1321 ggtttagacg ttgtttcttc catgtttgag gagagcttga agatcacacc ttatacacag
     1381 ttggctaatc tgactggaca accagcaatc agcttgccta ctcatttgac aaaagaaaac
     1441 atgcctctag gaatccaatt tatggctgcc agaggaagag aagatttgct atttcaaatc
     1501 ggctatctat ttgaagcaaa tcatcagttc catttaccaa atccatggaa atagcagaaa
     1561 caaggagaag aagaatgaag aaaaaaagat tgtttgctct aataactagt gcctttttta
     1621 ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga taacagctgg agtaatgtag
     1681 aaaaaaagaa agaattagta gtagcgactt ctggcacact gtttcccgca tcttattata
     1741 atgatcaaaa tgaattagtt ggttatgatg tagatttggc aaaagaggtg gctaaacggt
     1801 taaatgtcaa aatctccttt aaagaataca acgtggatgg acaaatcact tctgtttcaa
     1861 aaagagaatc agattttgct gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat
     1921 tctccctttc agagactttc aaatattcat ttgacagtat gatcgtacga aaaaaagatg
     1981 attcaggaat ccactcactt gaagatctta aagggaaaaa agcagctggc gaacccaata
     2041 ctagctatat gcgtttagcg gaaaaacacg gagctaaatt agtcacatat gacaatgcca
     2101 cgaatgatca gtatttaagc gatgtagcaa atggtcgaac agatgttatt ctaaatgatt
     2161 attatctgca gaaaatggcc gtatctgctt taccagatat ccctgtaaaa atactggaga
     2221 acgtttattt caatgaaaac gaaagtggtt ttttattgaa agaaggaaat gatgctctga
     2281 aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat
     2341 ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg
     2401 tgagtgagtg atgtactttc ctctaattga tttttccctc atgtggactt cacttccttt
     2461 cgtactgcaa ggcttggtat acacattagg gattggcttc gtgtcgtttc ttttaggaaa
     2521 tctggtaggg ttgcttttga cagttttagg tcttttggat tggctgcctc tgaatgtatt
     2581 tattcgtttt tatttatcgt tctttagagg cattcccgca ctagtcctgc tgtttctgtt
     2641 atattttggg ttaccttatc aattaagtgc cttaacggct tcagtcatct gctttacgat
     2701 taccagcagt gcatttattg gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg
     2761 tcagtgggac gctggtttag cttctggttt ttctttcttt ggggtattga aatatatcgt
     2821 tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt aacgtagcta tggatctagt
     2881 gaaaggtact tcgttagcag caacaatcac agtgccagaa atatttcaaa aagcaaagat
     2941 cataggcggt cggacattcg attatttttc tctctatatt ttagttgcac tgatttattg
     3001 ggtcatttgt atcgggatcg aaaaagtgca acagtattta gaaaaacgtt ctatgcatca
     3061 gtcaatctga aaaatagaaa tggtgctagc gtggctggca ccatttctat tttttgtttc
     3121 ttgtatattc tgcctagtat tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt
     3181 tcggaggtag aaaacatgaa gaccccgcta accatcgaaa tagaatcctc gttataccaa
     3241 tattgcatcg agcaaggcgg cttggttgta gaagaagtca cgatgcctga tgaccaagga
     3301 attgtagata cgcttagctg cttgacaaaa tttgatggaa gccgagaatg gcgatgctat
     3361 gagctgaaag tgacaaaagc agattttcgc tctacagcca aactgtcatt tgttggacat
     3421 tataattatt ttgttttacc aagtaaacta tatgaagaag taaaaactga aatccctaca
     3481 ggaataggtg ttttagtgta tcgtccgtac acggttgaag aggagatgcc tgcctctggt
     3541 acatttctga ttgcgaaaaa agcagcaaag aaagagcttg ctgtaccgga agaagctttg
     3601 acaaaccgct tcatggcttc tttattcagg gaagtaagaa aagcaaagca aatggagcga
     3661 ggcgtaaaat attttccaac agatcaattg tacaaagaat tgaaaagacg ttcagaaaat
     3721 caagatccat tttctaacga acgatattat gaacggtttt tagaggaaat agctagtcag
     3781 gcaatcaatg agttgcaaga agaagttatc gcgcttcagc atgattatga atatttgagg
     3841 caacaacagc agatccgtcg gttgcctaca gagccattgg agtgaaaaat atgtactatc
     3901 cttatttaag aggaaaacaa tttgacctat tagcattaaa agaagcactg tcgcgaggat
     3961 tgctctccaa taaaatccaa ccagtgatcg aaccagtacg tgattccgct acgcttaaaa
     4021 atgtgatcga attatttcaa aaaaagcacc atccaatcgc tgtgatccaa aatccccaag
     4081 taggacaatt caaattattc gatcagcatg tgcacagttg gacagtcaaa gaaaatagct
     4141 ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag tgaagtactg gattcacctc
     4201 cagcgtttct tgttttcgat ggacagcact acccaaaaga tacagaggtg tggaaacagt
     4261 tggcaggagt tgattcgaaa tttttgatac cagatactag tcgctttcgc atctggctgc
     4321 ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg
     4381 cagataaaac agatgacttt tttagtgatg actatctttt tttccacgca gacggatata
     4441 ttggattttc tgatttcacg atcgaaggta gccgttattt cgataaaggg ttccctagcc
     4501 gagcgcttgc tatccatcta acgtatattg atgcttacgg caatatacgc gtgaaacatt
     4561 ttgtctctga cagtaacgac tctgctaaag accaagcatt gaagtttctt gaagcaggag
     4621 ataaaatgaa aaaatggatc atgcgtcacc atcatcaact cttgattact tcaggaatga
     4681 ttgaattatt ggctctttac cagcaacaaa aattccctgg actgggagtg ttgaaaaagt
     4741 ggtcacttat gcatcatttg gaacttatta gtcacctgct agatcatcca aaagattggc
     4801 ttaaatatta ttcaaaagtt caggaactat atgaagtgat ccaatgatta gataatgtaa
     4861 atgaaaaagg aatagaaaag aggaaactgg atgtatattc ggcaaacaag gaaagaagaa
     4921 gtagaagaag tggtagaaat cattaatgcc gcagcggatt tcattgcttc gcaaggctct
     4981 cctcaatggc aaaacgggca gaaaccaaca aaagaaacga ttttgaacga tattgaaaga
     5041 aaagaaagct atgtattaat agtggaagat caaattgctg gaacagctgc attagtctca
     5101 agcgtggatc ccgtttacac aaaaatagat ggtagttgga aagaatcaac tggtccttat
     5161 ttatcgattc atcgtgtcgc tttagcagaa agtactcgag gaaaaaaact agcaaaaccg
     5221 ttcttagaac atttgttgac gattgcagcc agcaaaaata tccaagatat ccgtatcgat
     5281 acttatccag ctaatgaacc gatggaaaag accatctatt ctgctggatt cacttatcaa
     5341 gggatgattg aatttccttt tgaacacggc gaaagaaaag catatcaaat aatcatcaat
     5401 tgactataaa atcatctgag caaagataaa gacattaaca ctactgaaaa aaacaatcat
     5461 atgatagcca agaggttgta acagaagtgg ccagcttcaa aaaacaagaa agaatttccg
     5521 aaaatgcttc tcaaattttt gtggaatttt agcgtatttt acgaaatagc tgctttctga
     5581 aactcccttc atcgagaggt cgtgacatag cttttgtcac gacctctcgg cttatcctac
     5641 gagtattatt cgatacattt atatcaataa tccttttttt attttatctg cagcttcttg
     5701 gaacgctcca tcgtttgaca cgactaaaag tgaggggata gctttcaaac gtttcaactg
     5761 tgctaaatct tctacataaa ccgccagtct ggctgaaatc tgttcagatg aatctccacg
     5821 ttttttcagg cgcgattgca atgtttcttt agagatcgta ataaagatag gacggactac
     5881 cgtcccgaaa tactcatata aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt
     5941 tcctactgtc agtttctttt taatttctgc tttactggaa ccatatttat gttgagcata
     6001 ttggtcccac tctgcaaact catttccctt gatcatctga ttgaatgtat tgtcagaaac
     6061 aaaataataa tcctcttgat ctttttcccc aggtcttggt gatcgattcg tgtaagtaat
     6121 gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa gtttttccag aaccagaagg
     6181 accgataaat acatagcaat aattcattgt catttctcct ttgatgattg aacaagtgaa
     6241 aaagctttgg aaaatatttc caaagctttt aaaagtaact ttgctttttt gtatattata
     6301 ccagcaagcc agtcacaata gcacttaaaa agctgactaa agtcgctgtt agcaaaattt
     6361 tcaatccgtg ttttgcgaca agatttcctt tttcttcatt taatcccttc atcgcaccag
     6421 aaatgatccc gatagaagag aagttggcaa atgaaacgag gaagacagat ataattgctg
     6481 ttgtacgctc agtaaaatgg aaattaccat ttgctaattc tgtcatagca acaaattcat
     6541 ttgtaaccag tttagtcgcc atgatactac cggcatccac tgcttctttc catgggattc
     6601 cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat ctcttggaaa gtgatgccga
     6661 atatcccgtg gaagatagca ttgatcatgg caatcaaagc aacaaaaccg ataagcatag
     6721 cagcaaccgt gatcgccaca tggaaaccat caagaatgta ttctcccaac atttggaaaa
     6781 atgtctgttc tttgttttct tcaatgatca gctcatcttc tttttcttct aatacatagg
     6841 gatttacaat tgaagccaga atataaccgc caaataagtt caaaacaagg gcagtgatca
     6901 catatttaga atcgatcagt gccatataag atccgacgat cgacatagaa actgtagaca
     6961 tagccgatac actcatggtg aacaaacgtt tctcagatag gaagggtaat tcctttttta
     7021 tggaaataaa aacttccgat tgtcctaaaa tagcagaagc tactccatta taagattcca
     7081 atttgcccat cccgttgatt ttacttaatc ctagaccaac agctttcata aataatggaa
     7141 gaatcttgat atagcgtaga ataccgataa tagcagaaat aaatacaatt ggcatcaata
     7201 cggaaagaaa gaaaggtgca cttcctttat ttgctacacc ggcaaaaaca aagtcaacac
     7261 cttgcccagc aaaagcaaga agatgatcaa acatttttgc caatacagaa acaaccgctg
     7321 taccaaaagt tgtacgtaat aatacgaaac caagaacaag ctgtaaaacg aacatcacaa
     7381 ttagtggttt caatttgatt gcttttttgt tactgctgat caaaaaagca agccctaatg
     7441 tgacaaataa accaataata cctaataaat aactcaaata atcactccta ttccaaattc
     7501 cgttttattt tacctgtttt gcaaataaaa tactaatctt tagtgaaatt ttcattactt
     7561 ttttagagtg aaacgcattc attttccatt agaaaataaa gcgaacaaaa taaaaggttt
     7621 aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa actatcgatt aagctaatag
     7681 tttcgttttc aacatttata agtaaacttt ttctattggt aatatagatt ttcggaaggg
     7741 tagacaggtg cgcaggtaat atcgattcct agttttttca atgtttgttc atcattttta
     7801 ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt cagctagttt atcataagca
     7861 acttgagcga ttggattcgt tactgcactg attgataaag cgatcaagac ttcattgatg
     7921 cttagtgcat ccaaagagga atgaagtgtc tttgatttca atccttgtat tgtttcaaga
     7981 actaatggtg acaataaagg aatgtcatct gcgatatggg ctaaatgttt tactgcattt
     8041 aagataactg ccgctgacgc atccatcaat gaacttgtac gcccggtaat gatgacttca
     8101 tctggtaatt cgattgcaat cacagcagaa ggatcttttg taggactttg cgcttgaatc
     8161 ttatctgcat agtcatgggc agggactacc acgtttcggt cttcaggacg taaaccgact
     8221 tcttccataa tcagtttgat tcgattaact gcttcttcgt cgatcaatcc ttttttgaag
     8281 tcgcattctg tttggaaata acgacggatg atttcttgtt tagaggcttc ttggacaacg
     8341 gtatcatcca cgatcccaaa tcctacacga ttcacaccca tgtcagtagg agattgataa
     8401 acagattctt taccagtaat tttttcaata atacgtttga ttacaggaaa tgtctcaaca
     8461 tcccgattat agttcacagc tactttattg taggcatcaa agtggaagga atcgatcata
     8521 ttgacatctt ttagatcgac tgtggctgct tcataagcaa tgttcagcgg atgtttcaac
     8581 ggaacattcc atactgggaa agtttcgaac ttggagtaac cagctgcatg tcctttttgg
     8641 ctttcatgat acagttggtt caaggaagta gcgagctttc cacttcctgg gccaggagct
     8701 gtcactacaa cgattggttt agttgtttca atatactcat tttttccaaa accatcttct
     8761 gagacgattt tttctacgtt gactggatag tcttcgattt ctgagtgttt atagaccttg
     8821 atgtttcgtc tttctagttt attgataaag actttcgtgg caggctgtcc actatagcga
     8881 gtgataacga cactgttgat taataagcca taaccacgta attcatcgat taaccgcaag
     8941 atatccatat cataagtaat cccataatca ccacggatct tattacgttc aatatctcca
     9001 gcatagacgc aaatcagtat ttcagcttga tctttcaatt tttgcagcaa tttgattttc
     9061 gcatcttctt caaatcctgg aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca
     9121 ccaaattcta aatataattt gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc
     9181 tcttcaatat atttttctga atcaaaacca atttttttca ctaggttccc tccttaagta
     9241 ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa
     9301 aaacaaagaa aatggtttaa accaacttgt ctagatagtt attcattggt atgatagttt
     9361 ttgataagga aggtgctaca tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta
     9421 tatccgttcg atttttaccg gttatgaaag aatgcttgaa gatcccgaat tggaacaatt
     9481 gacgtttact tatctaaacg aagaaacgca gctaactcgt gatgagcatc agcgcattca
     9541 tttcacaaca agagatctgc ctagtaaata aaaaataaga taaaggtcat cggattcgat
     9601 tctgtcctat cagatggaaa gactagttga tcgaaaaaaa ggctgtacca aattttggtg
     9661 cagcctgttt tatggagcat tgcccgctct tttttataac aattttttac agaaaatggt
     9721 actattgaag tattaataag tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag
     9781 agccagatac aaatgcaaat tatgagccag acgaactaga ggagacgaat gcggatcagg
     9841 atacaggaca agaatcgtct tctttttacg atatcaaaga aaaagcaagc atgaatcttt
     9901 cccaattatt agaagactta caggcttttg aaaaagctgt ccgcgaagaa gatatccctg
     9961 agatctaccg tttatacaat ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa
    10021 atcacgagat cgatcaattg ctgatgcgta agatccatga tcgtttttta caggaatttc
    10081 cttttatgga acaagtagaa aacatctcgc caacgatgag ctattacaaa atcggcactt
    10141 attaccatga tcggcccacg gtcggtattg atgcaagtct gccagaaatt tttgttttgc
    10201 ctaagatcga tgaagagtgg gagaaatatt cccaaccgga gtcaacggac tatgacaaaa
    10261 aaatcaatga gctagacgca aaggtgatca ctgctcagac agaaattgaa cgtttagatg
    10321 aacaaataaa agaaatcaat cgacaaatga ctgatttgaa cgacaataaa ggattcttga
    10381 atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa gaagaagcaa gtactatcta
    10441 atgaaaaatt aggctggctt ccttatattg agacgcctga aacaatccag cagcaaaaag
    10501 aagcattgaa gcaagaagca agagcagacc agcttcgtgc agccatcgta gaaaaagaac
    10561 aaaggcaaat cagacgctat ttcggtagca aagagggatt cggtcaagca atccatgaat
    10621 tcttgatgaa ttatcttggc aatgagaatc ctaataatca atctaatgaa ggaggttccg
    10681 agtatgaatg aacacgatga agcagaatgg acaaaaaatg aagaacaaac agaagatcat
    10741 gaagaaacga acacttcatt ggaagaacaa acaagtaatc ctctggatga cacagaagaa
    10801 atccctatgc tagaggaaac aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg
    10861 aatactgcag aaaacaccca tgcttacgaa ataactgaaa atcccgggta tttagaagga
    10921 aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa cagcggaagc agaattagcg
    10981 gatttacgta tgagaaaaga tttgatcgaa tcagattttc cacaattatt agaatcatat
    11041 gatcgtttta tattgaatga agaaccaatc tccacgatcg gaaagacaag cctgatcgaa
    11101 tacttcacgt atgaattact gaaaccgttg aatgatattg gtctggaacg gtcaaattca
    11161 gcttatgaca catggaaaac aagacatttt tcgatcaatt atacgttgaa tggacaaaac
    11221 gaattagtct tttcctttgt attaccgacc atcgatcaac gataccaagt ggaatcgatg
    11281 agtctcctaa acgtatcacc agagacaatg gaaatcaatg tccaagatga ccgggtgctt
    11341 tctttgatcc gacactggtc agtggatcga gtattctctg cagggcagat cacgatattc
    11401 aatcacaaat tgaatcaatt attagcccat gcaagaacac ttggttttac cgttaatcag
    11461 acattactag acaatacgaa gccacttcgt ttgaatcttc agagcgaatt tgaattgacg
    11521 aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa atccatccta cgatatggaa
    11581 aagatcaacg aaactactta tcaagtacga ttagataaag gacagtcttt aacgataaca
    11641 acaaacgaaa aagatcaaac agtcttgtcg atctcatctg gagactaccc acgatctgtc
    11701 attgatttct ttatcaatta cgaattcttg gtcccattaa tggtgagaaa gtcctgattg
    11761 gataagttgg attaccagat gtcattcaga taattctgtt ccaaaggact tgcggcagtt
    11821 atccagaata attgtcacaa gtcttttctg tatgtactaa atggacgtga ataacttgct
    11881 gatgaaggag ggatttcatt aggaatgtcc acaaataaaa tagcacagct tagcttattg
    11941 tctgctgctt gtgtagcagg taggatcgct tttcaattta ttccgaattt ccagccaatg
    12001 actgctattt ttttatttat agtactttac ttaagcttaa aagatgcgtt aattgtcatg
    12061 agtctttcta ttgcgatcag cagtttttat tttggtgttg gaccatgggt catcggccaa
    12121 tggatcagct atactgttgt tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg
    12181 caaaggaatc tatggttaaa gggagtctgt ttttttctgg cgggcatggt ttacggaata
    12241 ggtatgaccg tttttgatac gctgttatat cgtttgcctc aaccttggat ctattacctt
    12301 caaggggtgt cgtttgattt gatgcacagt ataggcaatg ttgtgttctt tcttgttttt
    12361 ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa aaaatgaaaa aaataattta
    12421 tagtttgctt gcaatgagtg tcagtatact cattttgaca agttgcagta caacctatac
    12481 acaatcggct tccaaccaaa aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt
    12541 acaagaaaat ggtcaaaact tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt
    12601 actgagtatt ttgaaacgaa attttgaagt caaagaagat aacggcttca tcacttcgct
    12661 tgaagggcac tcacaggatg agaaaaatca actctattgg atgtttactg tggacgggaa
    12721 aagtgctact actggtgcca aagagatcaa attgaatgat ggacaatcgg tcattttcaa
    12781 tttgtctaaa ctataagttt cataaaaaac tgttaaaggg cgaatattgc cataattcag
    12841 tgagttcagt tgatgaaaga aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt
    12901 ttccgtaatt gctgctctag gggctaatat tctagttgct atcagcaaat ttgtcggcta
    12961 tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag
    13021 caatcagatt ttgctgttat tcggtgataa acaagccaat aaaggacaga gcgagttgca
    13081 tcaatttggt gaaggacgag caaaatactt tttcagtaca atcgtcgcaa tgatgctttt
    13141 cttcggcggt ggggcacttg gggtgatgga agcttttgaa aagttgacgc atccatccca
    13201 tgaagtgggt aatccgatca ttgttatagc aatactgtta tttggtttgg taatcgaagg
    13261 aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg aatactgaaa aactttccac
    13321 gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg attattttta cggaagactt
    13381 gtgtgctgta atcggtttgg tcttggcttt agcaggaaca atattgactt atgcaaccgg
    13441 gattgctgca tttgatgcga tcagcggatt gctgatcggt ttcttgctga tgggagccgc
    13501 cattttccta gcaaaagaat tttatagtct gatcatcggt gaaagcgtga cagcaagcga
    13561 tttatcaaaa atcaagttag cttttgaacg tcctgagatc agtcggctga tcgatataaa
    13621 aacagttcat ttaagtccaa cggagatttt agttgcagca aaagcagata tcgttggtaa
    13681 caaagagaat gaagcttact cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct
    13741 tcctgacaag aagatgtata tatacattga aacagataaa tatgacccaa attattctcg
    13801 taaataaaac gtttattcat gaattgaagc aaaaatgaga aaaatataat cttgttttta
    13861 aataaaacgg ctacaattta gcgaatttat gttacaatga gtccgctgtt tatttgttgt
    13921 aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca
    13981 tgcagtgatg ccactttgta taagctatat accagtagga ttagcttgcg gagtattgtt
    14041 gcaaaaagtt ggatttgatc ctctcttatc aggactgtta tcgattctgg tcttttccgg
    14101 tggcgcgcag tttttagttg catctatgct tacgacgcaa gcatcatttg caacgacatt
    14161 attgatggtt ttctttttag aattacgcta tacattatta ggagcaagtc ttgccggttt
    14221 tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct caatcactga atgatgaaaa
    14281 ctatgcggtg aactacttaa agttttcaac tgacccttcc tggaataaaa gaaaggcttt
    14341 atatgtcaat tggttctcaa tgactgcatg ggccggtagc aatatgatcg gtaatttttt
    14401 cggctcggtg attcgagtag atgctgacct tgtccatttt gctttgacgg ccatgttcat
    14461 tttcatgttt atcatgcaga tgaaaagcgc actactgatc tttacaggtt tattctcagg
    14521 cgtattaggg gtcgtattca tggtattatt ccaaaatact ttcggattga ttgcagcaac
    14581 agtcattgct tcatttgccg gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa
    14641 gaaactaaga gaccgcggta aagatgtcaa tgagccgctc ttcaacttcc cggaacagga
    14701 ggccaatcac catgactaaa ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg
    14761 catatatccc gcgcttgttc ccgatgcttt attttactca tcgaaaagtg ccatcctggt
    14821 tcagtgactg gatgaaatat gtaccggtgg cattatttgc ggcgttagct tttaaagatg
    14881 ttttcattac tcatgaacat ctggatattg cttggaatat caaaatagct gcaatgattc
    14941 tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt gtcagttttg acaggacttg
    15001 cgtctgtctt tttgctgtct atgctttaaa ctgttttatt ttattaaaaa cagagattgc
    15061 gacataagtt gttcaaactg atctatccga actgcatttg caacagtcaa ttctggatgc
    15121 aagtttattc gtagggataa aaaagattag tgttggctta tgatgcaagc tcttttttat
    15181 tttaggaatt agagagggat ttgtccttct atgatttttt gctataatag ggaagatgtt
    15241 tattttatga aagaatagtt gagggaattt aaatgaataa aaaattgatg caaggaacat
    15301 tttggctaac ctttgctaat cttctatgta aagttttagg ggtagtctat ttgattccct
    15361 ggctaagtat gatggggaac aatcaagatg gtatgcttgc tacgacattg tataatgttg
    15421 gctatcttcc ttatggattg ttcctgatgc ttggtacagt cggatttcca aatgcgattg
    15481 caaaaaaagt agctgtagcc acaaaagaag gagataacgc tgcttgtcgg ctgattttta
    15541 gaagtacgat caatatcatg tttgtcatcg gtatcgcctc tgctgcactg atgtatttat
    15601 ttgctccttt actagccgga atcagtccga ttgctaacgt taataatggg atactagcta
    15661 tcagaagttt atgcccatct ttgattgcta tcccaattct tagtgcaatg agaggatatt
    15721 tccaaggaaa aaatgatctg cggccttatg gaacttcttt gattatcgaa caggctgtgc
    15781 gagtgatcgt tatcttggct ggtacttatt acttgcgcgt attgactgac ggaaccatat
    15841 tagaagctgt attgatcagt acagtcgctt ctttcttcgg tgggttagct gcgattatcc
    15901 atatgtatat cgtcggacgc agaaaagact tcttcgaact taaagatttt ttgatttcca
    15961 gccgttattt cgaaagagaa aaccgatctg cctctgtctc tatcattcgg gaaacactac
    16021 cgtttatttt tgtcggctca gtcattacta tcgttcaaat gatcgaccaa gtaacgatga
    16081 aaccactgct tcactttttc cgtccagaga tcgcagatca gcaactggaa ttcttattca
    16141 gccgtgcttc tgtcaatcca aataaattga cattgatcct gatctccatg gtaggaacag
    16201 tagctataag cagcttgcca atattaagca cgatgaagaa aaaagatcgg atacaaatcg
    16261 aaaaaacagt aggagatagt ttttcgattg cgttattgat tcttcttcct tcattgactg
    16321 gaatgtcttt attagctgga ccactataca cattgttttt tggctatgat cctgaaagtg
    16381 tcggatattt ccaaatggct ttgctagctt ctttgttttt ctctttattc acgatcctat
    16441 ctacgatgct tcaatctttg aatcaccatc gttttgcgat ccgattaact gtagaagcaa
    16501 ttatcctgaa agtcatcttc caagtaatcg gattaggatt agttggcgga tacgggatga
    16561 gcttatcaaa tggcttagca ttcggtattg tctttgttcg cggctatcac tttatgtgca
    16621 aagaataccg aatagcacct ttagcaaaga tcagcaattt tttcttaaaa actttccgct
    16681 ccaccctgat tatgcttgca gtatgttttg ccgttttctt tttgctgaat cagcgattgt
    16741 caatggaatc aaaaagctat gcaatgattt attgtgtggc tgtaggaagt attggcggct
    16801 tagtattttt attttctcaa ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac
    16861 atcatagcag aagcaaagaa taggttaact aatcgttttt tgctgtcact tgaaaaacgg
    16921 caactcgtat gattacgctt gtcgtttttc tattttccaa ttttagtttc atatctaatc
    16981 ttacttatga tatagttata aggtctggaa aaattctttg aaacgaggaa attaaacaca
    17041 tgaaacttgc agtagttaca gatagttcgg catatctgcc ggaacgtatc aagaaccatc
    17101 ccgacttatt cgttattcca atccctttga ttatggatgg gaaaatttat aacgaaggaa
    17161 tcgatatcga agcggatgaa tattatggac ttttaaaaaa cagtaaagat tttcctacta
    17221 cttcacaacc tgtcgtaggg gaagttttgg gtttatatga ggaattgaaa gagaaagggt
    17281 atgacacgat tttaagtatc catctttctt cagggatttc tggctttgtc aacacattat
    17341 ttgcgatgaa agatgacatc agcggagtaa cggtcattcc ttatgactct aagatcacta
    17401 gtatgccgat gggacacatg gtagaagctg ccttagactt aaatgatgaa ggtcagtccc
    17461 ttgatgaaat acttgcacat atcgatcgaa tccgagataa tacctacgct tatctgattg
    17521 tagatgattt gaacaattta gtacgaggag gacgtttgac aaatggtgca gcgctgattg
    17581 gcagcttatt aaaaatcaaa ccgatcctga cattttctga agggaaaatt gttttattcg
    17641 aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga agaaattatt ggtagaagaa
    17701 ataacgaaat acaaccgcca gtcaaacttt acgtgataca tgcaaataat ttagaagtag
    17761 cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc
    17821 atttcggacc agtcattggt acacatttag gtgaaaaagc catcggcatt gccatttcag
    17881 cgcaataatt ccttttattc aaaaaacata ccaaaaatat tccaacatca tcttggactt
    17941 tctacatgtt actaatgagc gtgtaaaaga acgagatggt gtttttattt ctatcaatga
    18001 agctcttttt ctttgcaatc ctcacttcaa tcctctataa tgtcgcatat agtttagtaa
    18061 atgagtaaac tactaattta cttatttact aaaaataaaa ggatgaggag ttaaaataat
    18121 ggaaatacca acaaatttaa aacataaacc agtaatcgtt gtagaagact atgataaagt
    18181 tgatggaaga aatgcattaa atacggatgc aaaagggctt tcactgggcc ttgctcagtg
    18241 gaatgatcgt ggaaaagtcg atatctcagg caaggtatgg cgacatactg gagaaaaatg
    18301 gtcacgacag tcggaagaat taccaatcac acgtatttta gatttagcga tcttagctgc
    18361 acaaggaagt ttatacttcc aagatgctta tcgttatgaa aaattttatg atccagaaaa
    18421 tccagtcgta gacatcattg gattacaagg gaaccgaatg acggtagaag tagataccaa
    18481 aaatccaaag atcgatgaag acatcatttt gtattacgat acactacaaa aagacggaga
    18541 actgattggg gaacgattcc gtattttaaa acgtctttta gatgagctag gatattaata
    18601 gacagagatg atcaaaaggg gaagagaagc atggagaatt tctcactaac agaaaaccga
    18661 accttttcac ggctagaaaa gcaaatgatc tggaagaaac cgaaaagcca tcggacaagt
    18721 gcagaagaat ggcggatcgc ctccgaaatc aaagggaatt ggcaagatcc tgaaatgaaa
    18781 atcagtaacg tacttttgga aggagatgcc gggtcgggga agacacaatt agccaaagca
    18841 ctatctgctg atctgcaact tccctataca aaagtgacct gttttgcaga tatggacaaa
    18901 tcagatgttt tcggagcctt attacctgta gtagattcta ataaggaaca agaccaagaa
    18961 ttactagaag ctatttacca aacagataca cttgaagctg tactgacact gatccagaac
    19021 cattacggca ttgatccttt ttctgctaaa gaaaaattag cagagctagt tcaaaggatc
    19081 gaagctgatt atccagatgc aatccattac agctattacc cttctgaaat cgttcgagcg
    19141 attgaaaaag gatatttatt agagattcaa gagccgactg tcatccgaga tgcctctgtt
    19201 cttgtggcac ttaactcagc gctcgaacca aatgggatgt tgaatttacc gacaggaatc
    19261 attcgtcgcc atccagattg cgtcattgtg atcactacaa atcgaaatta ccaaggaaat
    19321 cgcccattaa atgaatcttt aagagatcgg atgcaacatg cggaaaaaat ggatcttcct
    19381 gccttagaag tcatggcaga acgtgcgatg gcgaaaacta tgatccaaaa acaagaactt
    19441 ctcttaaaga tggcagagat tatccgtttg ttggatgaaa ctgcaaaagc taacgccatc
    19501 aaaggtgtag caggaatgcg ttcttatttt tattgggcaa atacctataa gcaaggacaa
    19561 gatttactcc agtcgattta tccaaaagtt ttgtataaat tatccactga ttcagatgaa
    19621 ttggcaattc ttgaacaagc tttgtctgac agtggattgc tactggaact tcaggacata
    19681 ttgagaaaag aaaaatgggg tcatcctcaa tctgagcata caaaaggaag aaccattagc
    19741 gccgaagaag caaatgaacg aaacatcact gaaaatgctg aagaaattct gcgcttagat
    19801 gaaaagaaat cgttgtctgc tgaagaacca aatgagggag aagagaaaca agaagaaaca
    19861 atagaaaaaa acgagattcc tcctcaaatg caaaaagaac gcagtagcga tatcgaagga
    19921 aaaaatcaaa aagaacaaca aacaggagat gattctcaag aaatgtctgc ttctgacatg
    19981 gaagcacatg ataaggaaat caagaagcaa ctgaataaag aagcgcgaca aatcatgaag
    20041 gaaacgattc atgaaaaaga aggtctcatc gttcaccgac cagctttcca aggaaaaacg
    20101 ccggaagcac gtgagctaca catgcaagta atgccaatag tagaatcact tagccggcaa
    20161 attttagagt tactggataa tgaacaaaca gagacgtacc aaaaaggaaa atacgaaggt
    20221 cagcggttca atgcaagcaa agtagcatat ggtgatttga gaaattttga caaaaagaac
    20281 ccgccacatg agcaaccttc tttagcggta gctgtacgga tcgatgaatc tgggtcaatg
    20341 atacgggatg atcggatcga agcggcaaaa aaagcagcga ttgccattgc cgaattttct
    20401 aagaaagtcg atatcccatt gttgatttac ggagatacag cagaccgttc gtcaagagaa
    20461 aagacttctt tgttttctta taaagaattt gaagacggat ttcaatggtt aaatgaaaaa
    20521 ttagtcacta tgaaaccaag gcaaaacaat cgtgatggtg ctgcacttcg tttagcagca
    20581 gaaaaattaa acagacaatc cgcaacaacc aaactgttgc ttaatattag tgacggtcag
    20641 ccaaaagcgt tgccggatta tacaggcaaa aaagcaaaag aagatatcca gtctgttttg
    20701 aaagaatatg aacgtcaagg aatcctattt gtctccgcag ctattggcca agacaaagaa
    20761 gaaatcaagt cgatctacgg tgaaagtcgt tttgtagata ttactgactt gaatgaattt
    20821 cccaagcaaa tgattcaact gattgcccgc tatctatgat aaaataaagc tcaacgaacg
    20881 cacttagcag attcgttgag cttttcgttt ctagttaccg gtaacaagga tacttctaaa
    20941 atagaatcgg ttataatcaa gagggaccgt taaaggagtg aaacgatatg atacaagaac
    21001 aaaaatggtg gaaagaagca gtaggctatc aaatctatcc tgctagtttc aaagatagca
    21061 atggcgatgg tcaaggagac atcaatggga tccgtgaaaa acttaattat ttgaaagatc
    21121 ttggtatcgg ctttatctgg atcaatccga tctatcaatc tccatttgtc gataatggtt
    21181 atgacgtaag tgactatcaa gatatagaag agagattcgg aacgatggaa gattttgatt
    21241 tattgctaaa agaagcacat gaattaggga tcaaagtgat tatggatctt gtcatcaacc
    21301 atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa atctaaagaa agcccgtatc
    21361 gcgattatta catttgggtc gacggggtag atggcaaaga accgaataat tggacatcga
    21421 ttttcggcgg ttctgcttgg gagtattctc atgaaactgg acagtattat ctgcatgttt
    21481 ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa attgaaagaa gaactattca
    21541 atatgatccg atggtggttg gataaaggga tagatggatt tcgtttagat gcgatttctc
    21601 atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa ccctttctca ccctttcaaa
    21661 atgtctcagg aatcgaagaa catttgaccg atttgaaaca tgtttttgaa gagtacgata
    21721 tcatgactgt cggagaagca agtggtgtca ctgcagaaga aggaccacaa tgggtaggga
    21781 aagacggtta tttcgatatg atattcgaat ttgatcatat ccatatctgg cagcaagaaa
    21841 aagaagggca attagatgtc cttaaattga aacttgcatt aagcgcttgg caaacttcat
    21901 tggatggtat cggatggaac gctttatata tggaaaacca tgatgttcct cgtgccgttt
    21961 cagtgtttgg agatactcgt cccgattttt gggctatgtc tgcaaaagca atcgctatga
    22021 tgtatttctt tttgcaaggt acgccattta tttatcaagg acaggaaatc ggaatgacga
    22081 atatgccctt tgaatcgatc gatcaagttg atgcagtaga ttcgaaaaga ctttataaaa
    22141 ggctgcttgc ggaaggtaag acaagagaag aagcattgga tatcattcgt gaaacaacga
    22201 gagacaacag caggacaccc atgcaatgga cttcggaaca atatgctggt ttttctacac
    22261 atgaaccttg gctgatcact aatccaaata ccaaaacgat caatgtagaa caacaagaat
    22321 atgaaccaga gtcggtcctg caattctata agaacatgat ccgcattaga caaaccaata
    22381 aaggtctgat atacggttct tataaagaat atcttcatga acatccacaa ctttatgtct
    22441 atgaaagata tttagaagat gaacaatatc ttattatggt aaacttaaca gaatcacttg
    22501 ctgactatga acttccaaaa gaagcagatc agtcctggac attactcttg tcaaattcct
    22561 ctagcggaga atttgaagct aaaggcatac tagcacctta tgaagctcgg ttatataaaa
    22621 caaacaagta aaacttcctc gttaaagttc attagttgga ctttgcgagg aagtttttca
    22681 gattgctctg tcaattactc atttcaataa ttcgctctag gaccaccgat atatttagga
    22741 cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt tcaaacgagg tctgacaggt
    22801 acttggacat gtttgatatg cataccgatt gatgtgtcac cgatatcgat accggcttgt
    22861 ccaactacgt gttccacttc gacaggatca ttgaactggt caaaagcagc aacagaacaa
    22921 gcacctccag catgtagtgc aggaacaaca gagacaattt cgaaattttt tgcttctgcc
    22981 actgttcgtt ctactactaa tgctcggttc aaatgttcac aaccttggac agctagcgca
    23041 atttctttag gatccaacaa ctcttttaac gtacggatga tccattgacc aacttccgca
    23101 ctggaatttt ttccaatatg tcctccaacg acttcgcttg tgctgcatcc taaaacaaaa
    23161 agatcacctt ttttcaagtg tgcctcagcc aatatgtcat taactatttc agtcaattgg
    23221 tcttttaatt ctttttctga caacttcatt ttctaaaacc ttctttttca attaatattg
    23281 cttccgtgct tgaatacgtt gaagacttgc agttatagga aaggcaagtc ccattccaat
    23341 cagattttgg aagatgtttc ctggtataga agccactcca gccggccaat catataaaat
    23401 ccaaccggcg ataccatagc cgatgatcat aactaagctt cctaataaaa gccctaatcc
    23461 ccacatacga gtagaagatt tgctgaaaga accagcaaca tatccttgta acccgtggat
    23521 cactaatgaa aaaactgccc actgcggata tcctgaaatc aaatcgatca atagaccact
    23581 ggaagcacca acaactaatc cacccgttgg accataaaga aaagcagcgg tatagatccc
    23641 ggcctcgcat aatgtcacaa atccattcgt agccggtaca gggatcaaaa ttgttaatga
    23701 aagggcaact gtcaacgcaa tcagcatagc atagatcgtt aattgcttag ttttcatttc
    23761 tcattcctcc tttttgaata ccgccagacc agacacttcc ggtatgatcg ttcatcagca
    23821 cgccattaga aatacaatgg gcaacaaatt ttttactttc tgctactgct ttggtaaaat
    23881 ctgcccctaa cacaagattc gctgttattg cagaagaaaa ggcacaaccg gccccgttta
    23941 ctgttgtagt gtcgattttt ttatgagaaa atacctcata atgatcaatt gaatagagaa
    24001 gatccacagc ttcatctcca gccatccttg tgccgccttt gatcacaatg ttttttgttc
    24061 ctgaagcagt gagttcttta gcgagatgga tcatttctgt taacgaagag ggaagagctt
    24121 gaccagaaag taaagcagct tctgccaaat taggcgtaat catcgtaaca agtggaaaaa
    24181 gttcttcgat gagctgcgaa atatacaatt gattgatcac agcttcagtt tctttgaatg
    24241 ccaaaacagg gtctaaaatg attgggatat tttgttttgt ttttatgaag tctcgaacaa
    24301 ccatgattgc ttcacttgaa ttcaataacc caattttgat accatctaat gaagtcattt
    24361 tatcaatcga aactaactgt cgtttaagca attccaaaga tatagattcc agcataaatc
    24421 gctcattaat atctagtgcc cctatagaag tcaaggcgct tagtccgaat agaccaaaat
    24481 tctcaaaagt ttttaaatct gtttggattc ctccaccagc aaatggatca gaaccgccaa
    24541 ttgtcaagat tcttttcata acattttcct ccatgaataa gagtataacg aaattttcaa
    24601 aaaagaaaaa cagccaattg gcatattttt atctcatcca atttaaaaaa acaaaaagaa
    24661 aagaaagacc aaaatggatc tttctttcct taaatagtta ggacttttgt gattttaatt
    24721 ctttctcgat ttgaagccgt tgcactttca ttgtagctgt ccgcgggatt gcatcaaaat
    24781 cacggatgat agggacgttc aagcgaggaa gttcatggac ttgctcccac catcgattcc
    24841 aatccatttc tttatcagga gctaaagcaa taatcggttg tggagaatga tttttatccc
    24901 ttacaatcac tacttctgcc aaaaaatcta acgaatctaa taaaaagtct tcaatagcta
    24961 atgtactatt gatcgtttcg atcaaatcca cttgccgatc tttcaaaaat aggtggccat
    25021 gcttatccat aaaaccgtaa tctccgctat cccaccaatc gccataaaca ttttcctgga
    25081 accgtgcatc ttccttgtaa taagtcaaag cccgtccttt agacagtaat tgtatatgac
    25141 catctgtgtt ttcaggtagg acattcccaa ctgaatctgt aatccgagcg cttgtcatgt
    25201 cttccagtcc aacgcccata tctcttgcat cacttgtttt caaactctct agtgtatgag
    25261 ccttaagaat cattggtccg cattcacttt ggccatatac ttgaagaaat accgcatcct
    25321 gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt gtttatagca tcaaaagtgg
    25381 agtgatagta tcggataccc gaaaaagctt ctggatgctc cttagcagta aaacgccact
    25441 gaacaaagtt attcggatgg gtctctagtg caattggccg gtgagcttcc aacatatgaa
    25501 caacttttga actttgtgcg tttgctaaag gcatcatagg aaatcccatg gacatcaaag
    25561 aagagatacc aatattgaat ctggaatgca ctggagagat gtgaaaagcg attaatttct
    25621 tttccgaaat ttttgtgaaa actgtttttt gccatttcgt ccgccatccc atagaatgag
    25681 cagaatgaca aataagttta gggataccag ttgtaccaga agtatgggtc atataagaaa
    25741 tttcattttt gtctaaaaat gattgagaaa cagacaaagc aggttgttcc attaaataaa
    25801 gaataggaa
//
LOCUS       DAPBCS010000043        25655 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPBCS010000043 DAPBCS010000000
VERSION     DAPBCS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.043"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..274)
                     /locus_tag="RD521_002014"
     CDS             complement(<1..274)
                     /locus_tag="RD521_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7771723.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERALRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNL"
     gene            433..1161
                     /locus_tag="RD521_002015"
     CDS             433..1161
                     /locus_tag="RD521_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDT7771724.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKSCGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            1317..1634
                     /locus_tag="RD521_002016"
     CDS             1317..1634
                     /locus_tag="RD521_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7771725.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            1627..2235
                     /locus_tag="RD521_002017"
     CDS             1627..2235
                     /locus_tag="RD521_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDT7771726.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            2290..3081
                     /locus_tag="RD521_002018"
     CDS             2290..3081
                     /locus_tag="RD521_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDT7771727.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            3219..3707
                     /locus_tag="RD521_002019"
     CDS             3219..3707
                     /locus_tag="RD521_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDT7771728.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            3758..4630
                     /locus_tag="RD521_002020"
     CDS             3758..4630
                     /locus_tag="RD521_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDT7771729.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            4806..5168
                     /locus_tag="RD521_002021"
     CDS             4806..5168
                     /locus_tag="RD521_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7771730.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYIQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            5177..5929
                     /locus_tag="RD521_002022"
     CDS             5177..5929
                     /locus_tag="RD521_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771731.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            5943..7745
                     /locus_tag="RD521_002023"
     CDS             5943..7745
                     /locus_tag="RD521_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771732.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            8022..9062
                     /locus_tag="RD521_002024"
     CDS             8022..9062
                     /locus_tag="RD521_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDT7771733.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(9114..11258)
                     /locus_tag="RD521_002025"
     CDS             complement(9114..11258)
                     /locus_tag="RD521_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDT7771734.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGSDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(11615..12988)
                     /locus_tag="RD521_002026"
     CDS             complement(11615..12988)
                     /locus_tag="RD521_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7771735.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(12988..13383)
                     /gene="rbsD"
                     /locus_tag="RD521_002027"
     CDS             complement(12988..13383)
                     /gene="rbsD"
                     /locus_tag="RD521_002027"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDT7771736.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(13411..14328)
                     /gene="rbsK"
                     /locus_tag="RD521_002028"
     CDS             complement(13411..14328)
                     /gene="rbsK"
                     /locus_tag="RD521_002028"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDT7771737.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(14436..15449)
                     /locus_tag="RD521_002029"
     CDS             complement(14436..15449)
                     /locus_tag="RD521_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7771738.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(15530..16129)
                     /locus_tag="RD521_002030"
     CDS             complement(15530..16129)
                     /locus_tag="RD521_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7771739.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            16315..17016
                     /locus_tag="RD521_002031"
     CDS             16315..17016
                     /locus_tag="RD521_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771740.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17026..19737
                     /locus_tag="RD521_002032"
     CDS             17026..19737
                     /locus_tag="RD521_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771741.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            20188..21816
                     /gene="treC"
                     /locus_tag="RD521_002033"
     CDS             20188..21816
                     /gene="treC"
                     /locus_tag="RD521_002033"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDT7771742.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(21876..23057)
                     /locus_tag="RD521_002034"
     CDS             complement(21876..23057)
                     /locus_tag="RD521_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7771743.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            23309..24832
                     /locus_tag="RD521_002035"
     CDS             23309..24832
                     /locus_tag="RD521_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDT7771744.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(24901..25629)
                     /locus_tag="RD521_002036"
     CDS             complement(24901..25629)
                     /locus_tag="RD521_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771745.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         8263 a         4538 c         5219 g         7635 t
ORIGIN      
        1 aaagattgtt gtctttcgta taggtttcaa gcacttcttg catagcatgg tattgttcta
       61 cgacattttt tacatatggg agcaataagc ttccagcttc ggtcaattcg atttttctaa
      121 gggcgcgttc gaataatttg acgtctaatt ctgtttctaa cgaaagaatc tgtttagaaa
      181 tatttccttg ggtggtgaat tgtcgatcag cagttttggt gaaattcatt gtttcggcta
      241 aatcaagaaa aacttcgaat ttatggatat ccatcatatt cctcctactt attccaataa
      301 agaatacttt aacacaaaaa atgaattggt gtaatcttct ctgtctgtta caatctttct
      361 tgcagcgaaa gcaataatta cagaaatttt tctgattttt ttcgcttcgt agaaaaagag
      421 aggagttttt tgatgccaac tggagttttg atcaatgtag gttccgtttt gtttggcgga
      481 ctaattggcg gattagtagg aaataaatta agtgaacatt ttaaagcaca gctgacgatg
      541 gtttttggtg tgtgttcgat ggggatggga atctattcca ttgcgccgat gaaaaatatg
      601 ccggctgtta tttttgcttt agtgatagga acagcgattg gtttgattgt tcatttaggt
      661 aatgggataa ataagggggc tgcgctgatg caagtaccta tttctaaaat cttccctagt
      721 gaaaagcttg gtatgaccca cgatgaattt atcagcacat tagtgacggt cattgttcta
      781 ttttgtgcaa gtggtacggg gatctatgga agtttagatt ctgggatgac aggagacagt
      841 acgattttga tttctaaatc tgtccttgat tttttcacag cagctatttt tgcgtgtaat
      901 ttagggtatg tcgtttctgt gatagcaatt ccccaattca ttatttttta tattttgttc
      961 ttattggcaa aatttattta tccattgact acaccagaca tgatcttaga ttttaaatct
     1021 tgcggcggat tcttgatggt cgctaccggt tttagaatga tcaacgtgaa aatgttccca
     1081 gtagctgaca tgatacctgc aatgatcgtt atcatgccac tgagctggat gtggacaaat
     1141 tggattatgc cactgttata ataaaaatca aagagaagcc attttttgct gactaaaaag
     1201 gatcaaatct tttagtcagt ttttttgtat ttgacaaact gattttgaaa ctatattata
     1261 tgttgtataa tatagattga aataaaatat tacacttgag aatagaaggt gacgtaatgc
     1321 acatccagat tccaactgtt cttcttgatg gtactgtgct agcagcatta agcaaggaag
     1381 atatgtatgg gtatgcctta acaaaaatcg tacaagaaag tctctctgtc agtgaatcta
     1441 cactatatcc ggttttgaga agattgaaaa aagaaggata tcttgagacc tatgatgaac
     1501 cttttgaagg cagaaaccgt cgatattact cgttgacacc aatgggaagt cgtcatttag
     1561 aagaaattat tgatgaatgg attgtttttc gagattcttt agaccggtta ttggaggaga
     1621 gcacaaatga atgaatattt gaatgaagtt gtggatcatc ttcaagaaat tcctgaagac
     1681 gatcgttttg atttgatcca gtattatgag gaatattttt tagattctgg aaaaacatta
     1741 gatcagatca ttgatgaata tggtacacca aagcagtttg ccttaaaatt aaaaatcagt
     1801 tacttttcag aagaagaaca agcagaaaac gagttagtga aaaatccaaa aagacaaatt
     1861 cgcctgattt ggttaatcat tatcggttta tttgcttctc ctgtcctcat tccactagca
     1921 ctggggttta tattggttat tgggggacta ttggttgctt tactagcgct catttttagt
     1981 atctatgttt tatgtatcag tatcttaggt gttggcctat tcatgatcat ctcaggaatc
     2041 agtgtgtttg gacaatcgat tctgagtgga ttattcttta tcggtgtcgg tctgttcgcc
     2101 acaggaagcg cagtatttat ttcgccactg ttgctgaaag gaacaaaagg gcttttttat
     2161 gtcatgatga actttgttaa atggatggga cgacgtttta ttacaaaaag agatctgcat
     2221 tcagcgggcg tttgaaggag gacacataaa ttgagattga aacatttttt gattaccggt
     2281 attggtctga tgcttactgg tggcctccta gcaggtgcaa cttatacatt aggcgctcaa
     2341 aagagtctga cttgggataa tggtcctcgg ttgatcgata tggttactga gacaaaaaaa
     2401 atcaaggatg caaacaaaat cgtcatccat gcagaaaatc aaacagtttc ggtcatacgc
     2461 ggattggatt ttgagataaa aacgacttat gataagacag aaaagccaga aataaaggag
     2521 aaaaatggcg tgttgactgt gaatgcagtc aaaaaatcaa caggtgcaat cattagttta
     2581 gaagacacaa gtgcacaaat cactattgtt gtccctgcag atcatgaatt atctgaattg
     2641 acaatcgaag ggaataatag tgcaattgat atcaacgaag tatcaatcaa aaaagtgaat
     2701 actaagacac atgatggctg ggtatcgttc aatgaattta caggaggaac aataactgcg
     2761 aaagatgaat taggctcact tcgattagat gaggccaatg tggataactt gatcgtcaca
     2821 ggtgaaaaca gcaacttcca tctagaagac agtttgatca gcaaagaagg aaaagtcatc
     2881 ctaaacggtg gcaatttatc tttgcatgag atggaaaaca gtggctatac tatccaaacg
     2941 tctggtgaat cttatattga aaaagactac cagccaataa caaatacttt gttagaacaa
     3001 ggacagaaaa agctaaagat cactgctaaa gacacatcga tcaatattac gacggatcaa
     3061 gaggacaatg aaacagattg atcctgtact caactggata ttttaagaaa aaacagcaaa
     3121 gatccttcca atctttgctg tttttcatcg cttaacgcac ataactatag taaaataaga
     3181 aagaaaatat ctaaaaagaa aacagggtga aagagtatat gaaggtagaa gaaggtcaaa
     3241 gagaaaagtt aaaaacagaa atggaaagat tacatactta tatcactcaa ctaagccaaa
     3301 ccttctatga tccagacaaa gaaaaagtga tggttaatta cccgaacaat tcagaaggtc
     3361 gccaattaga gcaagtgtac catgaagttt tcaaacattt attgacggtg aagaaagagc
     3421 tggattatta ttctttaccg attatcgata caggaatatt gaagtatgat gggaagaagg
     3481 aacgatttat ctttaaatct gttcgggaag atttgcctct ttcagcaggg atggatctag
     3541 aggtcttggt agaagactat tttacagaag aaaagcactg ggttcgaaca aaattggatt
     3601 atttaccgca ggcagccgga gggacacagg caagcggttg gtacatcact gaagacaaag
     3661 agttagaact agaaggtgta atggcgcgta ttcgaaaaaa aaattaaaaa agtaacatta
     3721 gatatttatt gaaggataaa ggagcgcagg aagtagcatg agagaaaaaa gagcatttta
     3781 tatcaatggt tatgcaggga ttgtcggatt ggtcgtatta gctttagttg gattgttttt
     3841 cttctattta ggtatgtggc aagcgaaagt ttgggcactt tttttaagta tttttctttg
     3901 gttgatcacg ttattgttgt taagttcggc aactgtagtc agtcctaatc aagcaaaagt
     3961 aattttgttt tttggacaat atttaggtac gattcgagaa aatggctttt tccttacgat
     4021 acctttggca caaaaaatga ctgtttcgtt gaaagttcgg aatttcaata gttctgtttt
     4081 aaaagtcaat gatttggatg gaaaccccat cgaaatctca gcagtagtag tctttaaagt
     4141 cgttgataca gcaaaggcat tattcgatgt agcgtattat caagattttg tagaaatcca
     4201 aagtgagaca gccattcggc atatcgctag ccaatacccg tatgatacat tcaatgagaa
     4261 tgatttgacg cttcgaggaa acacgacggc agtgtctgac gagttgcaaa aagagctgca
     4321 agagcgttta gcagtagcag gtgtcgaagt aatcgaaaca cgcttgaacc atttagccta
     4381 tgcaacagaa atcgctagtg ccatgctcca acgtcaacaa gcaaaagcca ttttgtctgc
     4441 tcgacaaacc attgttgaag gagctgtcac aatcactcaa atggccttag agcaaatcga
     4501 agatggacaa gaaatcaatt tcaccgatga tcggaaagtc caattgatca ataatttact
     4561 tgtctcaatc attacagaca aaggaacgca acctgttatc aatacaggcg acatgaaaga
     4621 ccgtaagtga catacttttt gtaagatagg aagaaggagt ttaggacgga ttattgtctt
     4681 gaactctttt tttagttgtt ttaaagaaaa tgacaaaatt gaaagaagaa tgatagttga
     4741 ttcgaaggca gaattgttcg cttttttcta taatcagtat ataaagtaag agagaagggg
     4801 aaaaagtgaa aaaacttctg atcggtctac ttgttttcat cgttgtggta cttgcagggg
     4861 tgaaaatcgc agattacgta gttatgggtg gagaagaata ctatatacaa attacgacag
     4921 atggtacaaa aacagtttca aaatcagact ctggacaaga aacagtcaac tatagatata
     4981 tcctgcctgg ctatacgaaa gagggtaagg aaaaagaact ggacttcaag ggacaattga
     5041 ctcgaccgct tcgtaaagga gcatatttaa aaatcacctg gaacaaaaat aaaggtgtca
     5101 cttcgtatga agaagtcgaa aaaaaagacg tgccgaaaac agcactggaa aaactaatta
     5161 aagggtaggg aaagaaatga acaaaatagt cgaagtacag aatgtaacaa aagtatacgg
     5221 aaaaaacaat gagaaaaaga cgcaagcatt aagcggcatc agcttcgatg tagaaaaagg
     5281 cgaatttatt gggatcatgg gcgcttctgg ttctggtaag tcgacacttt tgaatatttt
     5341 atccacatta gataaaccaa cagatgggca tatccgaatc aatcaaaaag atgtgaccac
     5401 attaaaagga aatcaactag cagattttcg tgcgaatgag attggcttta ttttccaaga
     5461 tttcaattta ttagaaagct tgacagcaca agaaaatatc gctgttcctc tttctttaca
     5521 aggcgtcaga ccaaaagaaa tcaagcaacg tgtccaaaaa attgcagaac gtttatccat
     5581 ctctcatatt ttggaaagct atccttctga gatttccggt ggacaaaaac aacgggtcgc
     5641 cgccgcacgt gcattgatca cacaaccaac gattctatta ggggatgagc ctacaggtgc
     5701 gctggattcc aaaagtgccc gggatctttt agatacgatg gacgaattga atacaaaaga
     5761 tcatgtatcg attcttcttg tgacgcatga tccgttttct gcaagttatt gccaacgtat
     5821 tttattcatc aaggatggcg gtatccatca agaagtgaaa cgtggagatc aaagccgtga
     5881 ttccttctat cgagaaatac tatctattct tggaaacttg gaacagtagg gggagattga
     5941 ctatgctttg gaaattatca cttactggga tcaagagccg tttaagagac tacttggtgt
     6001 tattctcagg attagttatg gcatctgcta ttttttacat gtttgaatca atggcaagca
     6061 acgaagcttt tttgaaaagt aatacgatcg ttgcggctac agttatcatt ttccgattcg
     6121 gttctgtttt actagggatc atcacattcg tttatatttt atatgcaaat tcctttttga
     6181 tgacgatgcg gcaaaaagac tatgcaatgt ttatgatgct gggagcaaaa ggcagaaaaa
     6241 tcgcacagat gattttttct gaaacattct tagtagggat cgcagctact ttagtgggtt
     6301 cagctgtcgg tgtaggattg acaagtatcg tccaccgatt attagtggat cagttagatc
     6361 tcaatatttc gcattttaca ccttttagta ttcacgggtt gttgatcact gttttgtttt
     6421 ttgctgtttt gtttttatta gctgctgttg tcaatgcttt ttcgattgtc aaaaaaccga
     6481 ttttagcatt gattcgtgcg gatcagacac caacaagaat caagcagcat aaattcttgt
     6541 ttttccttga agtagtactg ggaattgctt ttctaggctg cggttactat atgatgaaac
     6601 acgtggggac ctatcaagtc tttggtatag gggtagcctt agttacgatc gtattaggaa
     6661 cttatttcat tttccattct gtgatcattt tctttttatc attactaaag aaatcagaac
     6721 agatttcatt gaaaaaattg aacaatttca cattgtcaca gttaaacttt cgtattcgtg
     6781 aatatacaca aatgctctca atggtcgcta tgctgtttgc cttggcactg ggcgctttga
     6841 cggtaggatt aggttttaaa aatgaaattg cagacttgac agaaaacaat tctggatatg
     6901 atctagtgat gaataatcct cgggcagaag atcagcaaaa aatcaaagaa ctttcaccaa
     6961 cactttctgc ttcttatact caaaaagaag atgcgggaac gatttactat aatgcaagtg
     7021 aatttgcggc tcaaccattg atcatgataa aacatgaaga tggaacaccg ccgaagatca
     7081 cgaaagaaaa agttcctgct aaaaaaatga atgaactgac tgtacaagag gaacttcgaa
     7141 gctatgaatt gccggaacag caagaaaaag aaatcaaggt agtcagtcaa tcagaatttg
     7201 atcaactgaa cttgccacaa tccactttac agctagttca ggtaaaagac ttccaagcaa
     7261 acctgaaacc aatcgaaaca cttgttgctc aaaataaaat gaacaatcca tctttgcaaa
     7321 aagtagagta cagcaaccag aaatatgaaa tgtatgaagt gtataatggt ctattctctg
     7381 gttttgaatt tatgggattc ttcctaggtt tagctttctt gaccatgctt gctagctgtt
     7441 tgatgttcaa gatcttgtca ggaagcaaga gtgacattgc tcgttatgaa atgcttgaaa
     7501 agataggaac acgacgcggg ttgcttaaac aatcgatccg tagagaagta ggcgtgttat
     7561 tcttagcacc aggaatctta ggtgtagtcc atgtcttatt cggattacaa atgtttggtc
     7621 tattcatgca aaatccatac aaagatattt gggttccgtt tacgatattc ttcgtattgt
     7681 atttcatcta ctatgtactg acgacatggt tatatactgg aatcgtattg aaaaagagat
     7741 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca agcaaaaata gctcctcata
     7801 tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttgttcgga
     7861 aaaagaggtt gtgacaatat ttttgtcaca accttttttt cacggtcttt tattagaata
     7921 tagtaaattt tttctcgtta ctcacggaat aagctgttaa gaaaacggtt tccatgtata
     7981 ctggaatcag tgcaaatgaa agaaaagagg gtgcaaggaa catggaaatc aatgaacaaa
     8041 attttggcga aaactatcac ttgattacga tcacgaatca aaatgggaca tctttgtctg
     8101 tcagtgatct tggggcacgg gttgttcatt ttcaaacaat aatcgataat gaaaaacgag
     8161 agttgatctt aggttttgat gaagcaaaag aatatctaga aaaagaccct tatatcggtg
     8221 cttcaatcgg acgtatggct ggacggatta cgaatggcac attcaaatta gatgatgaaa
     8281 cgttcgaagt aaaaacagac ccggacactg gtcattgttt acatggtggt gcacctagct
     8341 ttgaagccaa gcagtggcat tatacagtca tcaatggtga aaacgaagca tcggtcattt
     8401 tctatcagac tagcccagat atggaaaacg gatttcctgg aactttagat gtagaagtac
     8461 ggtatacgct gacgaacgat gatacatggc gagtgacggt ccaagggatc agtgaccgca
     8521 aaacagtatt caatcctaca aatcatgtct atttcaattt aaccggtgat ccagcacagt
     8581 caatcgacga acatatactt tggttgaata gccgattatt tgctacgctc aataaagata
     8641 caacgcctac aggagaagta gtttcagtaa gtggcacagc atttgacttc caacgtccta
     8701 aaaaattagg tgaggtcttt gccagtgatt ttcgccaatc aatgatagtg ggcggcatcg
     8761 atcatccatt tttgttgaaa aaacaatctg gtttactaaa tgctgcggaa ttgacaagcc
     8821 cagatgaaaa agtatcgatc caagtaaaaa cagatgcttc aggaatcgtg atttttacag
     8881 caaattttgg agaagatgga ccggaaatgc gcgggaataa attagtaaat catggcggta
     8941 tcacatttga gactcaagaa ctgccaggag cggagcgact tgaaagtttt ggagatgtca
     9001 ctctggaacc taaccaagta cgcgagtttg taacggaata caaaataaat ataaagaaat
     9061 agaataaatt aaaaagggag tgtgacagtg tcacatcccc tttttaattt attctatttc
     9121 tttaaaggca gatcaagcca gaatgatttg tctaaactta acaaggttag tacaggaaca
     9181 gttgcagctt taactgtacg agtagttgtt tctggtatct gtcttgccgt aaaattgttc
     9241 cacaaatccc agattgaagc gacacggaag atggaagaag cgttgttgac gcgttttatc
     9301 gttgaaaatt ctaatcctgt gattggcacg ccatctgttg tccatttgat tggcacatcg
     9361 aatcgcttag acatattgag gatttcagcg tttgcttctt cagttgttgc tgcttctttt
     9421 ttcggttcat catattttgt caggtcgtac gcttcgatga tcgcgttcaa tttttgtgaa
     9481 tattggcgat ctgtagcata acgtccttcc aaatatttgg ttgcttcttt gtagtctttt
     9541 gtttccgact tccaagttgg tttgtagaaa tcaggattgc cagtcagtcc atttttgatc
     9601 aagtcggcat agtcttccaa actttccttg taagaaggat atttacggaa ttgtgcgcgg
     9661 atttggtagc tttgtccgga actatcttgt tcaagtgtat tgaaggtcac gctgcttcct
     9721 ttatatgatc ctttgatacc aaaaaggtta tgatttggtt cgcttgataa taagctgttt
     9781 ccagatccac tttcaaggat agcttgagcc atcatgactg atgcgtagat tccttctttt
     9841 tgcccgattt cctgtgcatc atctgcaatc tttttcaaga actgatcagt tgtttggttt
     9901 tgactgaaat ggaacgagcc atctaatcca tctaaaatag atgagccgcc tgtcagcgaa
     9961 cctgaagcag caggtaaacc tgctaagttt actcggacag caaaatcagg cgtgaaatct
    10021 gaaatagaag ttactcgaac tttatctcct ggttgaggcg catggataaa ttgtccgttc
    10081 ccaatataga tacctacatg gtgagtaaca ccggaagaag gtccccagaa gacgaggtcg
    10141 ccaggttggg cctgacttac tggaatctgc gtacctgcat attgctgttc gcctgtccag
    10201 ccaccgatat aatgaccggt tgctttcata tatacgtaat aagtcaaacc agaacagtca
    10261 aaagtatttg gaccttttgc tccccataca tatggttttc caagatgctt gaatgcttct
    10321 cgaacgattg cttgttggac attcgttgta tttgtaggca atacagcatc aattggtgat
    10381 tttacaacaa aatcagaaga agcagaagat ggattctcta taccgctttg atttgctgta
    10441 tttggacgag atgaagcatt gttcgttccg ccattcgatg aaccattgtt tggatggttt
    10501 ggttctctct gagtatttcc attgttattc gatccagatg ggtttgtttg attcgaagaa
    10561 ttcccgctgt tcgaattatt gttattattg tttgaattat ttgaaccatt attattgttg
    10621 ttattaccca catctggttt gtttggcgtt tccggatctg gtgtactagg gacttcttca
    10681 ctaggatcag ttggtttctc tgttgaagta tccgatccag ctttaaattc tgcagtaata
    10741 gataaattgt tcgtacctac tgtaaaggtc tttgcatcat aatttacttc tttggcacca
    10801 ttaacagcga ctgaagcaat ttgttcacct tcagctggcg taatagtgta agttaccttc
    10861 gtttccgcta ataaaccttc taccgttcca ctttccgtaa tatccaaagg ttgctgttca
    10921 ggggaatccg ctgtcgaagc agtcagttgg atcttaccaa tcaaagaagg agcaattgta
    10981 agggaatatg tctcggctgg cttttcattt tctgatggat cagccggatc aatttcagtt
    11041 ggctcggtcg atcctgttgt tgagttagtt gttccagttg ggtcttctgt aaaagaatcc
    11101 gttgtttcac tagtagctga gctggatgta gtcgtatctg ttgtgactgt ttgatcagct
    11161 atttgattat ctacggcaaa tacggtattg gtcgacaaaa cagacggagc aatcagccaa
    11221 gtagcagcta gcaaacgtat cgttttttta tttttcataa taaactagtc cttttccttt
    11281 cattgatttt ctgtcttatt cagcattcat tagacatttt ttggaacaga taatcgtgtc
    11341 gaaaagcttt ttcttttcat aaatctgtct gtgtattcta aataaaacaa tttgtaaata
    11401 agcaacgagc attcattcgt ttttctttga cgttcatttt attgcataga ccacctttgt
    11461 ttggggataa acaagtgggg ataaaaatgg ttatgcatat gtaaattata ccaaagtttg
    11521 gaaagtatca caaaaattta taaaaataat gatcctaaaa agaaaaagac tgaaatatgt
    11581 gataaacatc tcatacttca gtctttttca ttaactattc ataagacatt aggtcttcat
    11641 caaacccgct aacgagagta gtgatcattg ctacgactag actaacagcc agtcctgcta
    11701 agtaccagaa aaaagtaccg gaaatgaatg caggcaatgt ggtcagacta ccaaatacaa
    11761 aagcgatcgc tttcacttgg gcaaatccca ttacagctcc accagcagct gcaccaatgc
    11821 aagcagcaac aaatggtttt ttcaatggga tcgcaattcc gtaaacagtt ggttcggtca
    11881 ctcccgcaag taaaatcgaa gcaaaagcag aaccggagat cgaacgggtt tttgaacgtt
    11941 tacttcgcag aaatacacct aaagctgcac cagccattcc aaaattggct gctcctgtca
    12001 acggaacgat tcggtcgaac ccataagcag agatattact gatcatgaca gggtttacag
    12061 cccattggat accaaatatt accaaaatat tccaaccacc agcgatgatt gcgccactga
    12121 tcggtccgtt aatatccatc atccaagtaa ttgcggcagc taaaccttcg ccaaggtaaa
    12181 cacccgcagg cccgaatacc attaacgtca aaggaaccgt gataactaat gaaagtaaag
    12241 gtacaaatac taattgcata ttttcgcgaa tatatttttt gaggaatttt tcaacaaaag
    12301 aataaaagaa aatacctaaa atcgccggca tgacagtaga cgtatactgc atcagtacta
    12361 caggaatccc cagaaaatca gtagctgctt tgccactgtc tgaaagtaaa gaggtcagat
    12421 tcggttcaat cagtgctgca gcaatgacag ccgcaatata tccattcact ttaaacgttt
    12481 tggcagcaga aaaagcaaga aagattggca gaaaataaaa gacggcattc gacgcagcag
    12541 acaaaatatg ataagttccg cttgtttcac taagccagcc aatcgtagtc gctaaaacaa
    12601 ccagaccttt taatatacca gaaccggcaa acagcggaat gatcggcgta aagatttccg
    12661 atatcaaacc aaatagtttt tcgatcggat ttagcttttc tttcggagct gaatgatcga
    12721 tttgacgcgt caattccttg taagcaggag ccaatccctg atccatgatt atttctgcat
    12781 atctatcgag taaaatgaaa tacttaatgg aagggatttg ctgttttaat ttttctgcat
    12841 tggctactgg ctgttttttc aatttgatac gaagccgagt cgcacatgga aggacagaat
    12901 caatattgtc ttttcctccc aactgttcaa tcagcatatg aatctgctcg gacagtacca
    12961 tctgttcagg attcattgtc gtttccatta gaagaccact ccactcacta atataatatt
    13021 ggcgtaaggt gtcatttcgc ctgtacgaat aaatgcatga cagttattca actctttttt
    13081 catttcttca tgtgggataa agctaatcgg tgtatctggt aacaataatt tgattttttc
    13141 caatatttcc gggttcattg tctttatttc ttccgccaat tcaatcgatt gtacctcaag
    13201 ttcttctaaa acgttttcta aaacagataa aaaacttgga agaccgtttg agacagccaa
    13261 atctattttt tcagtaccaa aggggacagg catgcctgca tccccgatac ttaatttatc
    13321 aaagtgcccc atttgtgcga ttactcgtga aatatctgaa ttgattactt ttcctttttt
    13381 catgtttgtt tcttcctctc attgttaatt ttagactgct gattgttcta aagcttcgcg
    13441 gtaagggata gaaggttgcg caccataaga ctgcacagca acagaagagg ctagatttcc
    13501 ataaaggatc gcttctttca agttactaag gtctggctga agtgtgctgc ttaatgcgcc
    13561 aataaatgta tcacctgcgg ctgttgtgtc aacggcatcc actttaaaag cagggacgat
    13621 accgtgttct ttttctgtat gatagaaagc gccggcactg cctagtgtaa tgattactgt
    13681 tccaatacct aattcatgga gtttttcggc tgctgcgacc aaactgttat gatctgtcac
    13741 tctgattcct gtaatgattt ctgtttctgt ttcatttggt ataatgatat ctgtcttttc
    13801 aagtagttca gtaggaatcg tttcacgagc tggtgcagga ttcaggatcg ttgtttttcc
    13861 tgcgtctttt gcaatagaaa aagctaggat cgtcgcatct aagctacttt caaactgagc
    13921 aataataaag tcactgtttg taatcagttg cttgtttttt aggacttctt gttcatggaa
    13981 agcattattt gcccctgcat ggatcaagat actattttct cctgctgcgt ctaccataat
    14041 cattgcttgt ccggttgtgg cgccttggat ttcagctatc ccagatgtat cgatgttttc
    14101 ttttgtcagt agatctaata actgttgtcc ttcgctgtca gccccaacgc ctccaataaa
    14161 agatgtccgt gcgcctgaac gtttggctgc cactgcttgg ttggcacctt tacctccgcc
    14221 agcgtgaaaa atctcatgcg catgcatcgt ttcgcctggc ttgggcatgt tggttaaacg
    14281 aagggtagtg tccatattaa tacttccaat tactgtaacc gtgttcattt tacaaacctc
    14341 caactatctc atttattttg atgggaaaat tattttttaa aacgttttag taaaacgttt
    14401 taatagttcg ataaaaatat agcacacctt gaaagctatt tcaagggtgc agttgaaaat
    14461 cttttttcta atcgtacagg taatcttttt tcttcccatt ctttttctgg gaattggatc
    14521 cgatctaaca ataacttggc agatgtttgt cccaattcga agattggttg tgcgatcgtt
    14581 gtcagtttcg gcttgatata ctcgcacata tctatattat catagccaat aatgctgtag
    14641 tcttcaggga tgctttttcc agcttcctct aatccgcgat ataatccaaa agctaattca
    14701 tcatttaaag caaacacacc agttgcagca gaatcaagaa gttctgctgt gatctggtag
    14761 ccaccttgtt ttgagaactg tgtaggtaag aggatcagtt ggtcgtgagg gatttggtaa
    14821 acatcaagag cagatttaaa cccctcaatc cgagcgtgga cattttccgg tggattttca
    14881 ggatagacaa gggcgatcgt ttgatgtcct agactaagca agtgcatgcc cgctaggtaa
    14941 ccaccgcgaa aatcgtctgt tcgtacggca tcactaaatc cttcagattt tttctgatcc
    15001 aaaacaatga agggccggcc ttgttttttc aaattttcgt tgattgcatc tgtagagaca
    15061 gcagaagtcg cgataatgaa tccatccaca cctcgacgtg tgagctcggc tagatattcg
    15121 atttcttttt gatgatcaga atcagcatta cataatatcg tgacgaaatt ctgcttatac
    15181 aagatatcct caattcctcg catcagtgta ctgaagaatg gattcgtgat atcaggaacg
    15241 agtacaccaa ttgttttcgt ttccttggta atcatttgtc tagcaaaata gtcaggctga
    15301 tagcctaatt cttctttggc agctaaaact ttctgaacag ttttcggact gaatttctcc
    15361 gtatttccat ttaatatcaa ggagacagtt gtgattgaaa cgccggaaag tttagcgact
    15421 tctttgattg tgatcttttt tttcaccatg ttcatactcc tatctaaatc ctgtggttat
    15481 tttcccatga aatcaaaaag aagtaaatga aaatcacgtg tgccagtact tatttgcttt
    15541 gttggatagc ttcgattatt tgtttcgttg agcgtactcg tgcagttttc ggaaatatct
    15601 tttttatgaa caattgatgg gattctttgt cacgatcact gcaagcatct tcagcaagca
    15661 cgatatggta cccgtgttga aaggcatcta atgcagtagc atatacaccg tttgttgtcg
    15721 ctacaccgct taaaatgata gtttcgattc cacgccggcg aagttgaaga tcaagagaag
    15781 taccaaaaaa agcacttgga ttgtattttg tgatagtcaa catattatct gtatctttca
    15841 aagaatcctt caaaagtaaa tcagtgaatt ctggaggtac agaagcagtc attccaccca
    15901 tatcagtttg ttgactcaaa taagggaaag tggagatgtc tacgttcact aaaactttca
    15961 aggcagcggt acgtttcaat gttcttacta acaaatcatt gtttgtcaga atagatgctg
    16021 cagtgtgtgg agagagtgta ccgttattta cgatctcttt ttgcatgtcg atcgcgacaa
    16081 aagcagtttt tgataaatca aaaatcatat tttcctttaa atcattcatg atgttccttc
    16141 tttcaaataa caatgttttt tatgtaagta aatcataaat cgtttatttg tcaactaaat
    16201 taaaggattc ctttctttag tctagagaaa ttcagtggta aaataaataa gtgtgagaaa
    16261 acgcattcga tgcatagata tagcgagaaa tatcgtgaaa atggaggaat cagagtgaag
    16321 tatgtcgaag taaaaaatga atacaaacga tacaaaatgg gagaaacgac cattacagca
    16381 aatgacgata tttctttttc aattgaaaaa ggcgaattag tgattatact tggtccaagt
    16441 ggagcaggaa agtcaacgat tcttaatatt ttaggtggga tggatacacc tgatgaaggt
    16501 cagatcatta ttgatgacac agatattgcg caattttcag ataaacagct tactgcttac
    16561 cgacgaacag atgtagggtt cgttttccaa ttctacaatc tcgttcctaa tctaacagca
    16621 aaagaaaatg tggaattggc aacggaagtt tctcctgatg cgcttgatcc tgtagaggta
    16681 ttgaggcaag tgggcctagc tcatcgattg aataactttc cgtcccaatt atcaggaggc
    16741 gaacagcaga gggtttcaat tgctcgtgca ctagctaaaa atcccaaact tcttttatgt
    16801 gatgaaccga caggcgcgct ggattttgaa acgggcaagc aagtattgaa attattgcaa
    16861 aacgctagca gacaacacgg caataccgtt ttgatcatta cacataattc tgctttagcg
    16921 cccatcgctg atagagtcat tcacattaat gatgctaaag taaggtctgt tgaattaaat
    16981 gatcatcctt catccattga tgagattgta tggtaggggg attttatgaa aacaagaaca
    17041 tatcttaaag caggttttcg cgaaattcgg caatctaaag gacgattcat tgcaatcatc
    17101 cttattgtca tgctaggaac acttttattt gtaggtgtca aaactgctgg acctgtcatg
    17161 caaaaaacga tggatcacta tgttggtaca gcaggacttt ctgatttgca aatcgtttct
    17221 actggtggct tgacacaaaa agatattgct caagcagaaa agatcccaga tgcaaaagta
    17281 gaaacaggaa aacagatcta ttattccaat actggcaaaa acgaagtgat tcaaatattt
    17341 tcttataaca aaaataacaa gcagaacaag ctacaattaa ctgatggaaa actcccggaa
    17401 aaaccaaacc agcttgtatt ggatgaaaaa gcggaagatg aagggtataa aatcggtgat
    17461 atttatcaga ttgattcaga tgaattgaaa gaaaaagagt ataccatcac gggctttgtc
    17521 cgttctccat tgttcatcaa caatttggag agaggatacg cgaacgtcgg aaatggtagc
    17581 gtcgattatt ttgtctattt acctgaatcc gtttttaaaa cagatattta ttctgtctta
    17641 tatcttgatt ttacaaacgt aaagaatttg aatacgtaca gcaaagctta caaagataaa
    17701 atggagcaaa atcaagaaaa ggcagaaaag tatttacaag atcgacccga agaacgctta
    17761 gaagagctga aaaaatcggc caacgaagaa ttagaaccgg cacagcaaaa aatagcggat
    17821 ggcaaagcac aaacaacgca agcaagaaca cggttagaag atgctaaaaa acagttagcg
    17881 caacagtcag caactatcca gcagcttcct gaagcgcaac gaacaatcgc tgttcaaaca
    17941 ttatctcaac aagaagctca acttgctgaa caagaaaggc aattagttga gaaagaacgt
    18001 gaactggcct ctgcagagga agaattgaat cgaaatgcag aaaaaattga aaatttaact
    18061 aaaccatcat acctatacaa tgaacgttca gaaaatgtcg gttttcaaga atttggtgat
    18121 ctgtctgatc gtatcgctgc gattgccaat gtttttcctg ttttcttttt ctttattgcc
    18181 gcattgatca cctttacgac aatgacacgt atggtagaag aaaaccgcag ggaaatcggg
    18241 acattaaagg cgttaggtta tacgaaagtt gaaatcgcaa aaaaatatgc aatctatgca
    18301 agtctagcct cagggatagg tattgtttta ggaacgatat taggaacgaa tcttttgcca
    18361 aggatcattt ttgagctgtc taacgagcgg tatgatattg ggagtgcgct tattttttat
    18421 gactggccgc cgattttaca agcagctgcc gcattcttca ttgccgcatt tggcgctgca
    18481 atgatcgttt tattcaaaga tttaagagaa cggcctgctg cattgcttca accgaaagca
    18541 ccgaagccag ggaaacgcat ttttcttgaa tacatcactc cgctgtggtc acgccttagc
    18601 tttaatcaaa aaatcagtta ccgaaatctc ttccgatata agtcaagaat gcttatggcg
    18661 attatcggaa ttgctggatg tgccgggctg atggttgcag gcgtgggatt gaaagattct
    18721 ctaagttctg tttcagccaa acaatttgga ccgatcattg actatcaagc aatcgttaca
    18781 ttggattcat ccacggaaga tagtcgtcaa aaagcagcgg atatgctgca gaagcaagaa
    18841 aaagtaaatg agatcctagc tattcatact caaaccgtag aattgagaaa aaaaggacat
    18901 gcttcgcaaa gtctgacagt gatcgtacca agtaaaacag ctgatttgga tcgttttatc
    18961 catttaactg atcttgaaga gaacccacgc tccttgccta aaacaggaat cgctattacg
    19021 caaaaagtag cagaactatt tgacttatct acaggagatc atatttcgct ttatgatgaa
    19081 gatcaaaaag aatggaaagt cgaagtaaaa gaaatcgtga aaaactatct aggtagcttt
    19141 atttatatgt cccctgaata ttatgcttca gttactgatc aatcgatgat ggataatgca
    19201 tttttgctgc gtacagataa gatgacaaca aaagaagaag aaatgctttc cgagaaactg
    19261 cttgccacag acgctgtgac aaatacgtct ttcatgtcca aacaaatcga aacgcaagaa
    19321 gaatcaatgg cgaacttaga ctcggttgtc ttaatctttg tcgtattatc aggactgctg
    19381 gcttttatcg tcttgtataa tctaactaat atcaatattt ccgagcgtgt acgcgaatta
    19441 tctacaataa aagtccttgg attttttgat aaagaagtca cgatgtacat tgttcgagaa
    19501 aatatcatct ttactctttt aggaatcatc ggaggatttg gtgtaggata tatattgact
    19561 gacttcatct tgcaacaagc gtctatggag aatgtcatct ttccattagt gatcacatgg
    19621 tgggcttatg tcttatcagc aggattaact atcgtattta cggttattgt gatgattgtt
    19681 acccatttca aattaaagca tatcgatatg attgatgcgt tgaaatcgaa tgaataaaaa
    19741 cagaggttgt gaaatcagcg gtttgacctg agtattagag aaacaaacgg caaaatagtt
    19801 tttcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccatgaat
    19861 cagaggttgt aaaaaagccg tcctgcatcg agcaaaaaga atactcaaat aaaattagct
    19921 cctcatgttt tacatttgag aagactgatt gccgtagata aaagcttttg aacaccattt
    19981 atagaagaaa caagaatatt ttgctatttt aaacaagagg atagacaaat acatgtctga
    20041 tctcttgttt tttattgggc aacagacctc ttagaatgta taaagataaa ttttacaaac
    20101 gctttcggtt cagttataat aataaatgat aacttgtata tataagttgt ggtaggtaat
    20161 aaatcagttg aatggaggta aatatccatg acatttaaag acaaagtcat ttaccaaatc
    20221 tatcccaaat cttacaaaga cagtaatggt gatggaattg gagatttaaa aggaatcaaa
    20281 gaaaaactcc cgtatctgaa tgatctaggc atcgacatga tttggctaaa ccctatttat
    20341 ccaagccctc aaaaagacaa cggttatgat atctctgatt acacagccat cgatcctatc
    20401 tttggtacga tggaggaatt cgaacagcta atagccgaag caaagaaata ccggatagaa
    20461 atcatgctcg atatggtgct gaatcacgtt tcgacagaac atgaatggtt caaaaaagca
    20521 ctagcagggg accagcatta tcaggacttc ttcattttaa gagaagagcc aacggattgg
    20581 gtatcaaaat ttggtggaag tgcttggtcg ccatttggag atacggggaa atattatttg
    20641 cacttatatg atcggacaca agctgacttg aactggcgga accaagcagt aagagaggaa
    20701 ctattcaaag ttgttcgttt ttggatggaa aaaggggtaa aaggattccg tttcgatgtt
    20761 atcaatgtta tcggtaaaga cgaacaattg aaaaataata aagaaaatga tggcaaacct
    20821 gaatatacgg acaaaccaat cacccatacg tatctaaaag aattgaacca agcaacgttt
    20881 gggacggatt ccactatcat cacagtcggt gaaatgagct ctacgacgat cgaaaattgt
    20941 atcttgtata cagaacctag tagagaagaa ttatcaatgg tctttaattt ccatcatttg
    21001 aaagtcgatt atgaaaatgg gaataaatgg tctactcctc catttgattt tgcagaatta
    21061 aagcgactat ttcatacatg gggagaaaag atgagtgagg gtaacggctg gaatgctcta
    21121 ttttttaata atcatgatca gccacgtgcg ctcaatcgat ttgtagctat cgatcagtat
    21181 cgaaaacaag gagcagagat gctagcaacc atgatccatt tgaaccgtgg gacgccctat
    21241 atttatatgg gagaagaaat cggcatgatc gacccagact attcatcgat ttccgactat
    21301 attgacgtag aaagtttgaa tgcttataag ttactccttg aacaaggatt cacagaaaaa
    21361 gaagcgtttc gtcggatcaa agcaaaatct cgcgataact ctcgaacacc catgcaatgg
    21421 tcagacacag aacaagcagg atttactaca gggaaacctt ggttaaaagt tggaggcaag
    21481 ctagaagaga ttaatgtaga aaaagaacta tcttctgaag attctatttt gtcctattac
    21541 aagaaattga ttcggctgag aaaaacatat ccaatcgttg cacaaggaga ttatcatgct
    21601 tatgaagcaa atcatccgga agtttatggc tttcttcgcc aatttgaagg acagcagctg
    21661 cttgtattaa caaatttcta tgataaagat acaaggatcg ccatcccaga aggattcaca
    21721 gaagccaata ttcttatcga taattattcc gatactgttc ttcatcaaag tctggagcta
    21781 aaaccatacc aggcaattgc tttgctgaaa aattaaaaca aaaacacaaa acgtcgttcc
    21841 tacgagctat tctcgtaaga tcgacgtttt gtgttttatt taaaataatc ttcttcagaa
    21901 aatgaagagt tttcatactc tcctttatcg ccataaaatt tattgtagcg ttgtttgaat
    21961 ttacggaaaa taaaagtaac gagtaatttg actaacgtat agataggaat accaaaaata
    22021 acgcccacaa ttcccattag atcacccatg acaagtaaaa cgattaagat cgtaatcgga
    22081 tggatctgta aacgatcacc cattactcga ggaatcacca agtcaccgtg gagaagttgt
    22141 acggcaaacc agacaatgac aaatttgaca gccataaaag tagagtcttg gaatgcaatg
    22201 atcaaacctg gaaagaaggc gataaacggc ccaatatatg gaatgataca taacaaccca
    22261 gcagaaatag ctaaaacact agcaaattct aatccgatca gtaagaaaca aacccagtag
    22321 actgctccaa ggataaatga agcaatgatt tgccccttta gaaatgaacc caattgttga
    22381 ttagcaatct ttgtcaaatt tttaaaatct gtacgaaatg ctggtgggac aatcgctaaa
    22441 actgactgat aaaatttttg aggatctctc aaaaggaaaa aagcgacaat tggaccagta
    22501 aataaagtaa taaaaatagc tgtaacagtt gaaaaaatgt tgctgatccg ttgtgcgcca
    22561 ctttcccaat aatttccaat aaatccagct atatcagacg tcacgtcgtc aatagaagcg
    22621 aaaaattgat taaatgaatc ggcaaaaggt gttttctcca aaaattgtcg gagactctct
    22681 tgaaaatctt gaaacaaatc tggcaaacta ctgatcaatg tctttgcctg ttcttcaaga
    22741 aaaggaataa gccatatgct tccaagcccc aataaaaata aaataatcac ataaacgata
    22801 atgactgaca agttcctcga aaggtgcttg ctcaaacgtt tgatgactgg ttccaaaaga
    22861 taatacaaga tcagtgcaaa caataatggg ggcaagatag tagcgataat gattccgaca
    22921 ggcttgaata taaacgagat ctgatggaaa aaataaatga tcagcgcaag taagataact
    22981 attcctaaaa catagtaaga ggtttttcct cctaaaaagc ggatcaaacg attcgttgta
    23041 tctgtttctt ttttcactct ttttcctcct ttatctataa cttcattata gacaaaagcg
    23101 ggtataaaag gaaaatgata gaggttgatt gcaaaaatat cgtcaatcat tcatataaac
    23161 tttctcatca attaaaataa gaaaacgctt gacagttctt ttaaccatat gataaattaa
    23221 atcctgttgc taaaacgtaa ctattacggt ttgcgaaaag cgaatcggaa cattcaaatg
    23281 aaagaagatg agaagaggag tgtcataaat gaaacgattg ataggtatat tggccatgct
    23341 ggttttagca ggtatgttaa ctgcttgcgg ggcaagtggg aaagccgagg acagtaaaga
    23401 aaaattgagc gtgatgacga ccttttatcc catgtatgat tttacaaaag caatcgtcgg
    23461 ggatgaagga gaagtagagt tattgattcc agcagggact gattcccacg attatgaacc
    23521 ttctgctaaa gacatggcaa agatccaaga tacagatatc tttgtttata atgatgaaaa
    23581 catggaaaca tgggtccccg caatccaaaa aacattacaa gaaggcaatg tccatacgat
    23641 aaaagcgaca gaaggtatgc ttttacttcc gggaagtgaa gaaggacatg atcatgacca
    23701 tgaacacggg gaagaaggcc atacacatga gctagaccca catgtatggc tagctccaag
    23761 tttagcaatc aagcaggtag caaacatacg tgatcagctg attgaagctt acccagaaaa
    23821 acaggaagta tggacaaaaa atgcagcagc ctataccgag aaacttcaag cactccatca
    23881 gctttatcaa gaaaccttta agcaagcaaa acaaagatct tttgtcacgc aacatgctgc
    23941 attcaactat ctagcgttag aatatggatt gaatcaagta tcgattgctg gtctttcttc
    24001 tagtgaagaa ccgtctgcgg cgagaattgc tgaactgaaa cactttgtta aagaacatgg
    24061 aatcaactat atttattttg aagaaaacgc caaagatagt attgccagaa cgttagcaaa
    24121 cgaagcaggt gtatctttag aagtattgaa tcctttggaa ggattgacga atgaacaaat
    24181 agaaaatgga gaaaactatc tttctatcat ggaagcaaac ctcgaagcgt tgaaaaaaac
    24241 gacagagaca gaaaacccgc tagaagatac tttgaccccc aaaaaagaaa agagcgtcta
    24301 caacggttat tttgaagacg aggatgtaca agatcgctct ctttcagatt gggcaggaac
    24361 ttggcaaaca gtagatacgt atgtagaaga tgggactttt gatcctgttt ttgaatataa
    24421 agaaaaattg aatcaggata agacagcaca agaatataaa gaatattata cgaagggcta
    24481 tcaaacagat attgcttcaa tcaagatcag tgagaaagag atggcattta cgtttaagaa
    24541 tggaacaacg aaacgttcaa attatcgtta tgtagggaaa gaaatcttga cctacgaggc
    24601 aggaaaccgt ggcgtaaggt atctattcga agcagaagat aaggaaagtg gcgcttttag
    24661 atatattcaa ttcagtgatc acacgattgc accggctgac tccgaacatt ttcatttata
    24721 tgcaggaaat gaaagtcaag aagtattgct gaaagaaatg gagaattggc caacatttta
    24781 tccagataca atggatggac gtgaaattgc acaggaaatg cttttgcact gataggcaaa
    24841 aaagaaccga agggtttgga tcggctggtc atttagtcgg tccaaactat tcggttcttt
    24901 ttatgaaatc aatttcttta atccacggat agaggctggg atggtctcgc cgtttgccaa
    24961 cgtgacttct ctggtttttt tattaatcga ccgcacattt tgtgtattga taacatagga
    25021 tttatgacaa cggataaaat tccgatgcat tttctcgatt tctttgatct tctcatagaa
    25081 ttgtaattgg gcatgagtca aatggacttc cagtttatgt ggcgctccta gcgaagtaga
    25141 aaaatattgg atgtctttga tcggtaaaaa tttgatctca tcattaaact ctattttgaa
    25201 gatatctttt tggatttggg acatttgaaa atcttcgaaa attttgctca ggatatgctc
    25261 gacattttct tttaaatcat aaagatcttc tttgacgata taatcagtcg gttcgatgtt
    25321 gctttttaag attttcatcg acatttccgt atgggaagtc acatagatta tttttcctaa
    25381 aggatctaat tcacggatca atttaccgat cgctacacca tccatttcac tgtcttctag
    25441 ttcgatatct aaaaagtaaa taccaaaaga agaagttctg atcttggctg cattgagaag
    25501 ttcttccgga tcagaggtag aaatatggac ataactgtct aagttttcaa tcatgattct
    25561 atttttgatc accgttgata aaacttctaa ttgttttgga tcgtcttcac aaatataaat
    25621 gggaatcata tcattactcc tttattttat ttgta
//
LOCUS       DAPBCS010000044        25177 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPBCS010000044 DAPBCS010000000
VERSION     DAPBCS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.044"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1087
                     /locus_tag="RD521_002037"
     CDS             <1..1087
                     /locus_tag="RD521_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7771746.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="RD521_002038"
     CDS             complement(1305..2447)
                     /locus_tag="RD521_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7771747.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="RD521_002039"
     CDS             complement(2452..2637)
                     /locus_tag="RD521_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7771748.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="RD521_002040"
     CDS             complement(2991..3236)
                     /locus_tag="RD521_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771749.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="RD521_002041"
     CDS             complement(3230..3631)
                     /locus_tag="RD521_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7771750.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="RD521_002042"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="RD521_002042"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDT7771751.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="RD521_002043"
     CDS             complement(5649..7415)
                     /locus_tag="RD521_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDT7771752.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="RD521_002044"
     CDS             complement(7428..8222)
                     /locus_tag="RD521_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDT7771753.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="RD521_002045"
     CDS             complement(8232..9608)
                     /locus_tag="RD521_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7771754.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVNWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="RD521_002046"
     CDS             complement(9728..10375)
                     /locus_tag="RD521_002046"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDT7771755.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="RD521_002047"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="RD521_002047"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDT7771756.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="RD521_002048"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="RD521_002048"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDT7771757.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="RD521_002049"
     CDS             complement(13042..14673)
                     /locus_tag="RD521_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDT7771758.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15649
                     /locus_tag="RD521_002050"
                     /pseudo
     CDS             14977..15649
                     /locus_tag="RD521_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(16374..16709)
                     /locus_tag="RD521_002051"
                     /pseudo
     CDS             complement(16374..16709)
                     /locus_tag="RD521_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16806..17258)
                     /locus_tag="RD521_002052"
     CDS             complement(16806..17258)
                     /locus_tag="RD521_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771759.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17265..17813)
                     /locus_tag="RD521_002053"
     CDS             complement(17265..17813)
                     /locus_tag="RD521_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDT7771760.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17858..18190)
                     /locus_tag="RD521_002054"
     CDS             complement(17858..18190)
                     /locus_tag="RD521_002054"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771761.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18204..18461)
                     /locus_tag="RD521_002055"
     CDS             complement(18204..18461)
                     /locus_tag="RD521_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771762.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18656..19198)
                     /locus_tag="RD521_002056"
     CDS             complement(18656..19198)
                     /locus_tag="RD521_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771763.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(19219..20310)
                     /locus_tag="RD521_002057"
     CDS             complement(19219..20310)
                     /locus_tag="RD521_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDT7771764.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20318..22528)
                     /locus_tag="RD521_002058"
     CDS             complement(20318..22528)
                     /locus_tag="RD521_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771765.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22544..25072)
                     /locus_tag="RD521_002059"
     CDS             complement(22544..25072)
                     /locus_tag="RD521_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7771766.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
BASE COUNT         8031 a         5063 c         3433 g         8650 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaat taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaaat tagaagaaac actttctatt ggtcgtacac caatacgtga
    15121 agcactgatt caactccgac aacaaggtct tgttgataca attcctcaaa gcggaaatta
    15181 tatctcatta attgatttag attcggccaa aaatgctcga tttgtcagag aacatttgga
    15241 acgacaaatt atgttagagt gttgtgccaa aatgaataac caaaaaaaac ggattttaga
    15301 aactataatt tcagaacaag aaaaagctgt tagtcaaaaa aatgctcgta gtttttttca
    15361 taatgataat ttattccata aagcttgttt tgaaattgca gagcgagaag aagtatggca
    15421 atggttagaa aataacaata cacacttaga acgatttcga tggctacgag taaaaacgga
    15481 aggattaaag tgggaaaaaa ttatggaaca acattatcaa ctatttgagg cattaacaaa
    15541 acaagatcca gaagaagcta actttctaac atcgttgcat cttcatttaa tgcttaatga
    15601 gcaaaatatt gttttagcta ctcatccaaa atatttcaaa aatatataac ttttaactct
    15661 ttttaaatta aatggttgtt ctgatcagaa aaatatcgct tgttttctta ttatgtaata
    15721 tatttcataa actattctgc ataacaacta ttgtttgtac tttttacatt ggataactaa
    15781 ttatgactgc tgataacttt tgatattaaa ctagtatctc caatacaaat aaaaaataga
    15841 tatcttgttc atcaaaatta gttcgtggat acttaccttc atttacatca ttcagcggtt
    15901 tcccattttc ttttattcta ttatttcatt ttctaataga cttggcttca tttcgcaata
    15961 ctttttttca ataggcatca aattggtgtt gtttagatat ttagttgtag ttttgtgaca
    16021 tgataagtat gctccttttt ctcttttcca ctaatacaac gatcaaaggg tgcttttttc
    16081 aaaaaactag aaaaatcaaa ataagattcg tattttgatt ctatatatcc tgtttcaact
    16141 acatcttata tttcacaata gaataaatag ccttttgcat agctgcttaa cttataagaa
    16201 attgcaattt acttcttcaa agtgaaaacg aatatgagta aaataaaatt attcttctta
    16261 cagataatta aaaatagcgc gtaacaaaaa tcggcattca atcacataat taggattgat
    16321 gccgattact tatattctac gtatagtttt agtattctta ttttttatca ccagtaacca
    16381 attgagaaca gccctaaagc agaatagtct tttatctcaa aacgcttaat ttcaggctta
    16441 ggaatatgaa taatcttttg agtatattct ttctctttta atcttactga agaaatacca
    16501 ctgcctatct tttttacaaa tatttcattt ggtgtacaga ctaataaaac aatatttgta
    16561 atcaatcctt gcactaaaga attaaaagga agactattta aaacagggtg tgcataacta
    16621 taacatgtga agtagttatt ttgaacttta acttttaaaa actttaaaaa taatcttatt
    16681 tgttcttcta aacaatcatc tatactcata taatcactcc actaattaat ctccccaaaa
    16741 agacacagca ttaagagata taccaacaca acagtgcaaa taactctaca tcttccttcg
    16801 caaccttatt tcaataaacc ataaaaatta ttttttaata gaaactgaaa attaaaacta
    16861 gaaaaagtag ttttaccgaa tgtaagagca tcatcaccat caatatagaa atacattctt
    16921 ttccagttac atttaatttt taaacaaaat tctccccaaa aaggaacttt ttctagtgaa
    16981 aacttggtaa tctctttgta attaattctt gttaaagaat aattaaacac ttcattttta
    17041 cttgttattt tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca
    17101 tcgacaaaca gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa
    17161 catgccttag aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct
    17221 tgtttaaaag ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag
    17281 ctagcagcag ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca
    17341 gcttttccta attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta
    17401 aaagctttta atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct
    17461 ccaggaagag aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt
    17521 ctaggaacaa taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga
    17581 ctgattcgtt cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa
    17641 aaaccaactg tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa
    17701 tcaactgtat tcactttttc tgtggcatac acacttgttc cagtactaac aaaagtactt
    17761 aaacctaata agaaaacacc taaaacacca aatattttaa ttattctttt cataatttct
    17821 cctccataaa aaataagtta ctttttaaat atcttgttta tgaattcatt ccccaaaggc
    17881 cttttaagat tgatacagca gtccgttcac ttcttttaga cttgtcatac aagtctttta
    17941 aatgatccca tatcagacta gtttgtctat ctagcttcaa tccattaaac gctaatgaac
    18001 ggttcatttt ttctattact ttagataaag ctacagaaat ttttatacct tcacaacgcg
    18061 catcttggat ttcaataata gtatcatcaa ttagtttttg aatttcttgc ctatcaacat
    18121 attcaatctt agaaagagaa tcttttaaag ctactaatga ttcaacgatt tcttttttct
    18181 cttcagacat tttcttcctc cttctagtga cccgtaaagc catgtagagc agagtccatt
    18241 gaaccagtaa ttacactatc tacaacgcca ttccaattta ctctacattt tccatttttc
    18301 agatttgtcg gtccatacgg atagcctttt tgacattcaa tgttcggatt ataccagata
    18361 ggattatacc attcagccgc agaagcagat tcagtcgata caaacaatcc cacaccaaca
    18421 aatccaatac acatcacact atagccaatt agttttttca tctcgtatcc atctcctaaa
    18481 aaatagaata gttcttatag ttattcataa gacatatttt ttccgttttt gttttatcct
    18541 ttctgtccta taacaacttt tttcaagtaa agaatgacat agtttttctt aaaaaaacag
    18601 tctgtttttt tctatttctt cggtgtataa taaatatgag tgttcatttt ttattttaat
    18661 acgcactccg tacataataa atcttgatcg catcaatctt ttgagcttct tgatctactt
    18721 tcaattgaag taatacaaat tgagtaacct tattcattgt ttcttctcca aataacaaat
    18781 attgatccgg attctctata tcttgtgtca cctggtaaag tttcccagtt gttttcattt
    18841 gtacatgcgg gtctacatca atactattgt ctttaataca acgatctgtc atatagtccc
    18901 tcacagcttg attgcgttta taaacatctg tgaaattact ccattttcta gcaaaatctt
    18961 ttaagaaatt ttttgtcatt tcatctattt gcatttgttg cgctgtctcg tcttctaatg
    19021 aaccaatttg actgttactt tcagtacttt gaagagttcc acttgtttct tttactgctt
    19081 cgattgtttc ttctgtcgtt ctcaattgtg ttttttgttt ttcagaagaa aaccaatgac
    19141 aaccagtaag acctatcaaa cataaccaac taaatactaa aattatccct tttttcacgt
    19201 ttctagtcac tgcctttctt atttcacctt acgaaccaga ctacgaattt ttgtttgatt
    19261 gtacgtgcgt tgataccgag ccataatccg tccttgctcg gcattctgtt ccacactaga
    19321 gaaatgttgt ccgccatatg aaacatctgt tataattccg gtgtgaccat agattccagg
    19381 agacaatgca ccacctgctt gccaattcac cacatctcct gcttgtaaat cacttggttt
    19441 tgggttcatg attacttgcc aaccataggt tgtccagtta taatcttccc ctattaacat
    19501 cgcaaacatg tgtccagaac ccattaactg tgggccacct aattgagata cataccatgc
    19561 cgtaccacca taacattccc cattatttac ggatcgtcca atgatccgat ccaaaacctt
    19621 cgcattgcct ttactttttc ctgcaataag cgaagttttt tttgttggta ggctattcca
    19681 atgcttgatc gcaaaagtat catattccgt taagttatag gacgttatga tactcattgc
    19741 tcctcgataa tacggactcc cttcggtaat acctgctgcc gcatagcctc cacgacctag
    19801 aatcaataaa caacttccag gatctttatt gcccacacat tgattaaagt tgctttggct
    19861 cgccaccata aagccataat agtcataaca agcttttaag ttcggaaact tcatatagaa
    19921 accaccttca gaaccaccaa ttccacgtgc agaaccttgt gggtagggac aaccaggaaa
    19981 ccaggtaatc ccaaaaaagt tcgtatcgtt tcttccagat tgtgactgtc cataagctga
    20041 ttcatagcct aattgagaaa gaatcacact cggtaataac tgatacttta cagctgcacc
    20101 aactgctgct tgcgcatgtt catctgtgat tggaccacgt cctttaatct ctgaaaaaat
    20161 cggtaatcct tcggtatatt ccccattaaa agcgacagct agacctgtag cttctcctcc
    20221 atgctcagaa agttctcctg aaaaaaagaa tggcaataga aaaaagggaa gaataaagaa
    20281 aagaagacaa cctagacacc ctttcttcat ttcttcctca tcctttcttc gataatggtt
    20341 tttggactgt tcgtcctttt ggtaaagtaa aatcagttcc tggttttgcg tccaaaatcg
    20401 gcagtgtttt aggatccttt tctatttttt ccgattgctc tgttggctta tctaaagaag
    20461 aaaatgcagg ttgtaacgat tttggtgcgg aaatcgacgg tttactttca gacgaacgtt
    20521 gccccggttg ttgcacagaa cgatttcctg atagagtggc taccgacgaa ttaggaacag
    20581 agtattggtt caataactgc tgcattgctg gataactccc atttttcgcc tgatttttca
    20641 cttggcgaat ctgttgtgaa agagaaccag cagctgttaa ttgtggcacg ggtacttttc
    20701 cagaaggcaa cggaatcgat tgaccaacaa aagcgatacc tttatttaac aatggttttc
    20761 tttgattatt cccttgttgc ttactcaatt gtgccaatga ttgtacctgt tgttttggtc
    20821 ttactgaatt catggacgaa tttggaatca aaccatttga aagaccagaa ttccactctg
    20881 ttttcgattt ggtagaagac atttcaggct ctaccccatg ataacgaaca tgacgaccac
    20941 tataaggcaa ggcttgattt gaacgcccac ctagttcaaa gggattaacc acttgcctcc
    21001 cttttaccga ttcttgtggt gtacgttttt gttgaaactt ttgaaatcca tgtatcgctg
    21061 cagaagcgag tggattcgtt gctcgataca cattccctgc taatcctgca cctttgattg
    21121 ccaaattttg tccagcagtt gatagttgac caggcagttg tttcatttga gacaaagaat
    21181 gacttgctat gtttttcgtg ttgtttatag tttgatctgc agctcctaac cctagtttct
    21241 ttaataaatc tttccgtttt acaatgagta aaattgatag gagtgctttg acaaacatat
    21301 ttcctaaaaa accaaaaaca gtattcattg gaattaaatc gtctactatg ttatagatca
    21361 aaaatgccac caaaataatc acactgtaaa aagatttttg aaacatcaag cccaaaaagg
    21421 actttaaggt attcatgagc atataatcaa atactggaac aaaagaagcg agtaatgaaa
    21481 aaggaatcac aagtaataaa cacaacacca acatttgaaa gataaaacga atcatgccaa
    21541 tcactacaag tggtaatcca atacttagat taagcaatgg caccatagaa ccaacgactg
    21601 ctttataaac agctttagtt ggttgaatat agacacgata agccttcttt ttccctctat
    21661 cttctcctgc agcagtggtc acttttgtct caatatcaga taattcttca cttgacgctt
    21721 ccttggctag aaattcttgt ggttcaatct ctgcttgata caattcttct aaatttgttg
    21781 tcccataatt taacaatata tacggatcaa caacagccgc tttataataa agttggcgca
    21841 ctttttgaat ggcttccttt tgtgtcgttg gcactaaaac accattgaca tcttttgtcg
    21901 ccaattcatc tagactattt gtagcagaaa aaataaagcc ttctacctcc acggacaaat
    21961 catttaaccc tttggtaaaa gcttctcctt tactaggtac gttactacca tagccaaacc
    22021 aaaccattga aaaaatgaca acgaagagaa atcgaataaa caccattttt gcgtaatgtt
    22081 tccctctaaa tatacgaaga gtaaatagat ataacacata caagacgaca atccccgcac
    22141 caataggatc gtatattttt gaataaatag tatccgaaaa agtgaaaaac tggtgaatta
    22201 actcgttcat tgcagctcct tcatagagct tctcaatgac aaaatcaagg ccttgccaaa
    22261 tcaatttact taaactaaag aacatattcg tcatcatttt tagaaaatct gtgccatttg
    22321 ccccaaataa cattttgcct aatgaaaatt cactatcaga taaataagaa gaaaaggtat
    22381 caatatgaaa tttcccaatc tcatagatcg attgatccac ctgcttttct gccgcataca
    22441 ccaatccacc aacaaaaagc agtccgaatc caatcgttaa aaaaaacaaa acgcaccatt
    22501 tcttttcctt atgctttata taagccattg tttcacctca tttctattgc atgattaacg
    22561 tatcctgttc ttctacttga gagtgacttg ttttttccgt tgtttgtaat gcagcggtca
    22621 tttcttcaaa caaactgtgt acattgagtt ttcctacacg tccataggga tctaacatcc
    22681 aacactgtcc tttaatcatg tggctcattt cagctaagtt cgcttcattt acttccaaac
    22741 gtaaatgatt caaaatcttt tctcgctcac tgtcttcatc aaaagcaaag acgacaccaa
    22801 aattcccttt ttctttatct gaacctaagt caccaataaa ttgtgtatct aacactaatt
    22861 gattcatctg ggaacgcccc acacgtgcca ttgctgcaat aatactacgt cctgcgttcg
    22921 aattattaaa tacccatgct tctgtaaaat aaatcgctgt tttcttacta ctatctcgtt
    22981 ttccaaacat ttcacaaaaa cgacctaaac taatcatcaa acacaaagac tttcgatccg
    23041 catctgtata tagttctggt cggacattgt catttggtaa tttcaaatct tggatctcta
    23101 aaatcgttgt tttggcatta aagtctaatc ccgggttttc tccataagaa aatcctaaac
    23161 gtaaaatcga atcttctacc gtcaagcgta aaaactcacc atattctcga acctcttttt
    23221 gttccatttg ttctaaatag tcgaccacat gcatcaagcc aacttgttgt ccggcttctt
    23281 tttgaactaa aacttcttgg attcccttta ataccgcccc ttttaaaagg tgattggtac
    23341 ctaacggact taactcatca atcatatcct gggctacttg tttcgcatct actcgggata
    23401 aacaaacaat tggatctaat acaccataat tacggggatt tgtagcatct aacgtgacat
    23461 aactcatttt ttgcaaatgt tctacaaaca aagggtaatg tttctgataa tacgggtctt
    23521 gaatgagttt cgtaaaccat ttgcgctttt ctcctttggg atccacaaat aaactttgta
    23581 cattcataaa cgaactatac aaaaagagca agccaactaa aaaggtcttc cctttaccgg
    23641 tttttccagt tactgctaca tgaggcgcat caaacaacgc acctttgatt ccttccgcaa
    23701 tggctagaag attcaaaaag acaaaacgat ttgacgcata aattaattct tctaatggtg
    23761 ttttttctcc tcgagaacca ccatccccta atgtatcttg ccgaccaata taccagccgg
    23821 attccatacc taattctgat actgctgaaa acaaccattc gccaactgct tctacagttg
    23881 ttcggtgctt ccaatgtaac tgccgtccca atggttgccc taataaacat ttatagaaca
    23941 attctcgttg atcatttttt gcacggtgga tatggataat cgtatgaagt tgttgaataa
    24001 ccgttgcact attttgacga acttcttcaa ccgtttgacc gtatacaata aaacatgcga
    24061 tccaatcgac gacaggttgc ccattatcta ctcgttgctt taaatgtttt actaatcctc
    24121 gaacagttaa taagcgttca ctaggttctt catcattttg aaccaattcc ttatgctctg
    24181 ttttgaaccg ttccgatagc caattcagtt ttaaaccaat acctccctgt cctttcaagc
    24241 gtggatagtg aattttcatt cgcaattctg ctgggaaaga aagatcctga gcaaattgaa
    24301 atagatcaca atttctcata ttttcaggtg tttccccaat aggcaacaat gctaaataac
    24361 tactccctaa ctcatcttct aattgaagaa ggcctttatg ttccttcggt atacgtacat
    24421 acgttccttg attgtctttc agtacttccc aattattttg ttcttctact ttgtgagcca
    24481 ttcctcgaat atattgcaga cgattgagat acaacagctc tttttttact aagggacgaa
    24541 tagaaaagct ataaagtaat tgtgtcactt cttttacata aggatcactt tcagaaaaca
    24601 aagtttccaa atcatctact tctaatccaa ataagcgtgc taaacttcct gctaaatcta
    24661 accagattag atttattaca tcatgaaaac tctccaccgt tgctttttct ttgactttga
    24721 ttcctaaaat aaatgaagga cgatagactt gaccttctcg ttccaacaat gtcactgttt
    24781 cttttgcata atacgcacca attgcttgac tatcgggaga aaatcccttt cctaatgtgt
    24841 caaacttcgt ccgtaaattc attgttcgtg gatacatcac caactccaca tcttcataat
    24901 ctgctaaaag caacagtaaa cgagccatca tttcttttat ttcctgtgtc ttttttggtg
    24961 tgttttttga aattgttcgt ggaaaaacat gatagtaggc ccagatacaa tcctctccat
    25021 ttaacaaaca atttttcttg aatgctttta ctggttcttt tacaagcacc aatgaatttc
    25081 acctcccaat aaaaaaggaa caaccagttg attgtccctt tttctaatcc tctaaataaa
    25141 catacttatt cacaaaaagt aattcttcca atgcatt
//
LOCUS       DAPBCS010000045        24581 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPBCS010000045 DAPBCS010000000
VERSION     DAPBCS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.045"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(159..557)
                     /locus_tag="RD521_002060"
     CDS             complement(159..557)
                     /locus_tag="RD521_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDT7771767.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(554..1327)
                     /locus_tag="RD521_002061"
     CDS             complement(554..1327)
                     /locus_tag="RD521_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDT7771768.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1324..2007)
                     /locus_tag="RD521_002062"
     CDS             complement(1324..2007)
                     /locus_tag="RD521_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDT7771769.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2174..3061)
                     /locus_tag="RD521_002063"
     CDS             complement(2174..3061)
                     /locus_tag="RD521_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771770.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3058..3927)
                     /locus_tag="RD521_002064"
     CDS             complement(3058..3927)
                     /locus_tag="RD521_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7771771.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4064..5350)
                     /locus_tag="RD521_002065"
     CDS             complement(4064..5350)
                     /locus_tag="RD521_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7771772.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5623..6201
                     /locus_tag="RD521_002066"
     CDS             5623..6201
                     /locus_tag="RD521_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDT7771773.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6201..7130
                     /locus_tag="RD521_002067"
     CDS             6201..7130
                     /locus_tag="RD521_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7771774.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7127..8863
                     /locus_tag="RD521_002068"
     CDS             7127..8863
                     /locus_tag="RD521_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDT7771775.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8926..9762)
                     /gene="pheA"
                     /locus_tag="RD521_002069"
     CDS             complement(8926..9762)
                     /gene="pheA"
                     /locus_tag="RD521_002069"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDT7771776.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9759..10268)
                     /locus_tag="RD521_002070"
     CDS             complement(9759..10268)
                     /locus_tag="RD521_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDT7771777.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10261..11556)
                     /gene="aroA"
                     /locus_tag="RD521_002071"
     CDS             complement(10261..11556)
                     /gene="aroA"
                     /locus_tag="RD521_002071"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDT7771778.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11569..12660)
                     /locus_tag="RD521_002072"
     CDS             complement(11569..12660)
                     /locus_tag="RD521_002072"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDT7771779.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12677..13843)
                     /gene="aroC"
                     /locus_tag="RD521_002073"
     CDS             complement(12677..13843)
                     /gene="aroC"
                     /locus_tag="RD521_002073"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDT7771780.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13845..14909)
                     /gene="aroB"
                     /locus_tag="RD521_002074"
     CDS             complement(13845..14909)
                     /gene="aroB"
                     /locus_tag="RD521_002074"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDT7771781.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14942..15964)
                     /gene="aroF"
                     /locus_tag="RD521_002075"
     CDS             complement(14942..15964)
                     /gene="aroF"
                     /locus_tag="RD521_002075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDT7771782.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16623..17597)
                     /locus_tag="RD521_002076"
     CDS             complement(16623..17597)
                     /locus_tag="RD521_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDT7771783.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17578..19461)
                     /locus_tag="RD521_002077"
     CDS             complement(17578..19461)
                     /locus_tag="RD521_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDT7771784.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19479..21704)
                     /locus_tag="RD521_002078"
     CDS             complement(19479..21704)
                     /locus_tag="RD521_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDT7771785.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21819..22853
                     /locus_tag="RD521_002079"
     CDS             21819..22853
                     /locus_tag="RD521_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771786.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22920..23933)
                     /locus_tag="RD521_002080"
     CDS             complement(22920..23933)
                     /locus_tag="RD521_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7771787.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24011..24199)
                     /locus_tag="RD521_002081"
     CDS             complement(24011..24199)
                     /locus_tag="RD521_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDT7771788.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7065 a         5341 c         4397 g         7778 t
ORIGIN      
        1 taaaatagca tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta
       61 ctatttcata agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg
      121 ccttcttgtt tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta
      181 tcgttatttt catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta
      241 gtgataccaa tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg
      301 atgttgatgc cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat
      361 gtcaacacaa tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt
      421 acaccagaac ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag
      481 tcgataacat cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat
      541 gctacaagat ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt
      601 ctttttcatc ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca
      661 atgcaatcat tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg
      721 cagcgattgc cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc
      781 caccgttatc aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg
      841 ctagcccgta tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca
      901 tagacaatcc tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc
      961 ggattctccc ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag
     1021 caaaccctac taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca
     1081 atcccgcaat agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt
     1141 gcaggaaaag gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac
     1201 gctttttgcg catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg
     1261 ccactgccaa aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga
     1321 aactcattgg aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat
     1381 accgaccatt ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc
     1441 tccggcaagg gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc
     1501 aaaagcaatc ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta
     1561 gcctatagcc atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc
     1621 tagaaatact ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc
     1681 gtcagatata tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat
     1741 cacgatcccg atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat
     1801 atatttccct actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag
     1861 cgtcattccg atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc
     1921 taacggtgca aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc
     1981 aatcacttct ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt
     2041 aaaatgaaag agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata
     2101 aaataacaca cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc
     2161 ttattgtgtt tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa
     2221 caaaaaatac gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat
     2281 agtctggtgt ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag
     2341 gagattgcaa agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga
     2401 ttgcagccgc ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta
     2461 gttcttttag accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat
     2521 tttgtcgaaa gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga
     2581 aactgttgat tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca
     2641 tcaatgccgc aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc
     2701 gtttactttg ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca
     2761 cagtggtcac caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat
     2821 tggaaaacaa attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg
     2881 tgatatccac gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa
     2941 taaatgatag gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt
     3001 ctaatgcttg ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca
     3061 tcgactttct cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat
     3121 aacaaaggaa actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata
     3181 gatgtattga gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac
     3241 ttcatcaaaa agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg
     3301 ttttaaattt gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga
     3361 tgctgcttca taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt
     3421 gtatccagtc caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg
     3481 agtcaaccag ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag
     3541 gccagattga gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca
     3601 tggaagaaag atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag
     3661 gttggaaata aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa
     3721 tgtattaaaa aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa
     3781 cccgttaaat gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac
     3841 catcggaata aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc
     3901 gtttttgaat aaatctttct gcttcattct tttccctcca attctatcat tgattatcta
     3961 catgaaaaga caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt
     4021 ttgtctgatg aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg
     4081 cttctacttg tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc
     4141 gatgaagagc ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt
     4201 ctggtatttc agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt
     4261 cagaagcttc ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga
     4321 tttctttcgc aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat
     4381 ctttcgctga atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt
     4441 tagaagcttg cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt
     4501 ttccagattg gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag
     4561 aagctactgc tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga
     4621 ctagatcttc tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt
     4681 cttggatcgt gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa
     4741 tcatccttac tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt
     4801 ctttgacatc tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt
     4861 ctgtataacc tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat
     4921 cgaaaggaac accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa
     4981 aatgatcttc ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat
     5041 agccttgtat cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc
     5101 tcgctaaagc ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta
     5161 tctctgtttc ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg
     5221 tctcatccca cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc
     5281 cgcatcctgt taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc
     5341 tgatgttcat ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca
     5401 tcataccaaa atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa
     5461 aataataaaa aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa
     5521 gatcgaaatg gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa
     5581 gtatttttaa attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt
     5641 cttatcgatg atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag
     5701 cacgggtttc aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg
     5761 gtacatgcaa tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa
     5821 ttatcgcggc tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat
     5881 gatgaatttg atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag
     5941 cctcgattga gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa
     6001 aaaaagccca acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca
     6061 cagcagattg ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg
     6121 tttttctgtt atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac
     6181 agtcgatcac ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta
     6241 ccttttcgga agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt
     6301 ttgttcaaaa gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt
     6361 ttgcaatcca agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac
     6421 gtttctatca taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca
     6481 accacgagca gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag
     6541 ctgtcaaaga acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt
     6601 atttgaaaca tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc
     6661 atgttgggaa tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg
     6721 ctgagtattt cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact
     6781 tttctgcaaa tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc
     6841 aggaaaatta tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat
     6901 atagctatct ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa
     6961 aaaaagtccg tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg
     7021 aaatctgtga gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag
     7081 agacaggaat gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat
     7141 caaagagcat gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca
     7201 agccatcgtc atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt
     7261 caatcaatcg aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga
     7321 tacaatcgag aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc
     7381 caatgatcca gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa
     7441 tgactctctt tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa
     7501 tggaagaact tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca
     7561 atctgcgatt gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca
     7621 ccacggattg actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa
     7681 aaatacattg aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc
     7741 gaatcttttt caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga
     7801 acagacgatc atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa
     7861 aaaactaaca gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct
     7921 gtaccgccag gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat
     7981 gggcgtgatt tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt
     8041 ggtatttatg tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa
     8101 acggctagag gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca
     8161 attgctaggg aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac
     8221 gatccaagac gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca
     8281 gccccatttt atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac
     8341 agatgaagca gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa
     8401 aaaagaagaa gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct
     8461 tttgagtatg cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca
     8521 gtcattatct atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc
     8581 ttttcacaat aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct
     8641 tttattcgaa gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa
     8701 tgatcatttt tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg
     8761 cgatgcatat ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg
     8821 tctaccgctt cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg
     8881 atttttttat tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc
     8941 ttctactgtc agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat
     9001 ttcttggagc gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa
     9061 aaaatattct cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc
     9121 ccgccagcca aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt
     9181 caagatgaca ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac
     9241 gatccaaaaa cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa
     9301 tccaacatgc tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc
     9361 tgctacatac attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata
     9421 atgcgtttct aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt
     9481 aatctttgtt gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt
     9541 aacttcgact tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa
     9601 tgaattctct acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca
     9661 aacagggatg gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg
     9721 ataagtgaat gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct
     9781 ataatggtat cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca
     9841 ctttcttcat accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt
     9901 ccaagagcta aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc
     9961 gcttctgcct ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca
    10021 ctttggacaa tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa
    10081 atatctgact ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc
    10141 gacaattgga tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat
    10201 tgagctaacc ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg
    10261 ctagccatga gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata
    10321 agagattgaa acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc
    10381 tacttgcagc atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag
    10441 aggtgtcggc ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt
    10501 ctgtaattct tctgccaccg catcaattct gttggtttct tttactttta attcttctgc
    10561 gtctttgatg atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc
    10621 atcaatcaga cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt
    10681 gacacttaaa tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc
    10741 catctcctcc aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa
    10801 caacagttgg ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat
    10861 atcacctgga acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt
    10921 tttatcttcc actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg
    10981 ggaacgttct ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa
    11041 agcagatttg acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg
    11101 ggttcctgtc acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc
    11161 catctcgcgt aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc
    11221 gaacagagta gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt
    11281 accggagttt cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc
    11341 atgaatcttg atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg
    11401 taaagtactc agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc
    11461 aattgctcca aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt
    11521 ccctcttaat ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca
    11581 gcaatgataa ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt
    11641 tttaaaactg atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt
    11701 aatcaaagaa actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc
    11761 atctggcaca tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg
    11821 caactggtct cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc
    11881 agtcatgatc cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa
    11941 taatatctgc ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat
    12001 cctagtgata tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt
    12061 gaattgttct gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat
    12121 tcctgttata cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca
    12181 aaatagttct tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta
    12241 ataggcattt tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc
    12301 cattggatgt ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg
    12361 cttcgtactg ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac
    12421 atcaagataa gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc
    12481 tgcacctgtt tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc
    12541 acgggttttt gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat
    12601 acaaagtgcc atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat
    12661 cttatttgcc ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt
    12721 tcaactcaac aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg
    12781 ctacaacatt ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta
    12841 cacttgcttt gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg
    12901 taggaatggg tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt
    12961 cgaagccgcc tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct
    13021 catccatcac ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc
    13081 ctttaaaggc attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc
    13141 attgtacgta gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc
    13201 cgatcgtatc tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg
    13261 ctggatcaac aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga
    13321 tatttccagg tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga
    13381 tgtcaagttc ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct
    13441 ctcttgcaga ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc
    13501 ctaccaaatc cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt
    13561 caggcacttc tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca
    13621 ctaaagtgac aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac
    13681 gatctttttc gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt
    13741 cgtgattgat ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg
    13801 ttaattctgg tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag
    13861 taatctttga atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc
    13921 aagatgatat tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat
    13981 tcatctggat gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct
    14041 aaagcagctg tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct
    14101 accattccaa tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca
    14161 attgcatggc caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca
    14221 tcttcttcca ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt
    14281 tccagaagat ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct
    14341 atagctgctg atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt
    14401 tttagaacct caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt
    14461 ttcgctttgt ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt
    14521 agcgtagtcg gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct
    14581 gctagatcac ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca
    14641 ttatctgcta aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc
    14701 gccggtacga ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt
    14761 tctgcatata gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa
    14821 atcgtttgaa gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat
    14881 ttttggttat caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga
    14941 attaatcgag tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca
    15001 tgcgtgagta agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat
    15061 caggatggat ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg
    15121 gtggtacaag ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag
    15181 ttaatttttt catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt
    15241 aagtccgaat cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt
    15301 ctgcagcatt aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt
    15361 tacctgtttt accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct
    15421 gtaggatatc gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa
    15481 tcactttcat tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta
    15541 aaccttggaa agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg
    15601 cgcccccggc tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac
    15661 aaggaccagc catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga
    15721 ctgtattgcc aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag
    15781 cattttctac tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt
    15841 ttagtccaat cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt
    15901 cgatatgatc gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta
    15961 tcattgtttt tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt
    16021 ttcaaggtgg ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg
    16081 ctccttcttt cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat
    16141 tgcctctaca gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc
    16201 cactatagat gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat
    16261 agatacaaac ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag
    16321 ctaaagtaat acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa
    16381 ctgatgtaag aattgtacct agtatagaca taaaaaaatc gactgtcaat catttttatg
    16441 aaactttttt taattttctg atagttaact ttgtttttca gaaaaataac aattggtttt
    16501 attgaagaaa atagtcatga acaaaagtat ctgagaactt ttttactctc ttgttcatga
    16561 catatagaat aaagcagctt tgtcttgtaa actccgtttt tcaaataata atctaaagat
    16621 tgctactgaa taataaagcc gatttcatgg tcctcttcgc tgctgattcg gtaaggtgac
    16681 tcaacatccg ctccccagct attgataccc ccgactcctc ttacttttgc aaaaatcgta
    16741 agaaccgttc gtctttctgg cggtagttct tctatatgtg ttgcctgttc cagttcttct
    16801 gctttgaatg gtaaagcgga aaaagcaaat ggcgtatcct gctgttcaaa tcgcaacgaa
    16861 aaatcttctt gatctttatc cgtattatcc aacgtttgat ttcttgttag ttctacccat
    16921 ttcgtttcca tatgcatgcc gcattcttgg ggaacaaggt atggggaaac ggggagtcct
    16981 gccactttat aaataccttc gacaccacca gccatccgat ccggatatgt ttcccctgat
    17041 aggccttcat actcaaaaaa ttttgctttc gttggcataa tgaaccttaa acccaatgcg
    17101 ggtaattccg gtaatccttt ttgtccataa taatgcatat tgacaagcat tttaccctct
    17161 ccagcgattt ggtaggaaat tgtcacttgg gtttttggta ctgtgttgat ttccagtgta
    17221 aaaatgattt caaccgtttc agcgtattca ttacttgaat attgattatt tactggagca
    17281 atcggcaaat cgatcttttt attgtccacc agtacttcta tttctttgac ttttggatac
    17341 aatccggctc ctaaccacat accggattta aatccaaatc cgctacctct gtcattatcg
    17401 gtcgttgctc gccaaaaaac tggaagtgtt tcccggtata gccattcttt tccgttttta
    17461 ttgagcgatt ccagtccgcc tcttgtataa gaaaaaatat aatgcgtatt ccccgatttg
    17521 acacctaatg tacagtcgcc gaagacgatt gcaacttttt ccgtatccat atgactttta
    17581 tttactgtat ttttcataat aatatcttac ctcctgcatg gcgggttttt cttgtccatc
    17641 tgcaaaaacg atcccattcc ctgagaattc atagtcactt ggtttatcgt caaagtcacc
    17701 gccatagcgc atcacttctc tgcctgtgat tggatcagtc acttggatcg cctgatcttt
    17761 aaaatcccat atatagcctc cttggtacat ttcataacga tcaagcaacg tcatgtaaga
    17821 atccattcca cctaacgaat tccccatgtc atgcatatat tcacataaga tgaacggttt
    17881 ttccgcttta ttttcaaggt agtcaatgat ctcttctggt ggcgcataca tgcagctttt
    17941 tacatctgaa atcgaatctt cgaatgctgg atttggaaaa ttcccttcat aatggacaag
    18001 tcgatcgggg tctacttttt tgaagtaatc gttcatttgt cgaatgtttt cttccgcata
    18061 agattcattt ccaagagacc aaaaaagaat acttggatgg ttttttaatg tttcgaaatt
    18121 cgtttttgct cgatcaagaa cagccgcctg ccactgaggt actgcacctg gtacgttgta
    18181 agaaggctca aatgcgccca ttttttgcca tgagccatgg gattcgaggt tcgtttccgc
    18241 catcacatgg attcctgctt ggtcgcataa ataataccaa gtgatctggt tggggtaatg
    18301 agatgtccgt acgccgttga tctttgcatt ttttatgata gccaaatctt tttccatatc
    18361 ttctatagtg atggcgcgtc cttttttcgg atgccactca tgtcgattta ccccgttgat
    18421 aatcatccgt tttccattga agtagatgat tttatctttg atttcgattt tacgaaaacc
    18481 gaaggaatag tcaatatact ccgttactct tttttcttcg tctatcgtct ccaattcaag
    18541 cttatataaa ttgggctgaa aatgccccca cagtgccaca ttttcaaatc catcgatgcg
    18601 aatcgtcatt tgctctttta acggatattg ctgttgatag atttccttcc catcaggatc
    18661 tagtatcgct acattcagct gactgttttt ggctacttgc tcaaaagaca aatctacttc
    18721 aagtttgcca ttttgacatt tgtcatctaa gcgaggtttg atccaaagat cttctaaatg
    18781 gttttgtcct cgagcaacta agaagacatc tctgaagatc ccaaaaaatc ggaaaaagtc
    18841 ctgatcttcc aagaatgcag ctgtacttct tttgtaaacc tccacagcta gaatattttc
    18901 ttcttccaaa taaggtgtta aatcaaattc agacggagtg aacgaatctt cggcataccc
    18961 gataaaatga ccattcaacc aaataaacat tgcttgttct acaccagcaa aatgaatcga
    19021 gatttgtttt ccctttagac cttcttctaa tcgaaaagtc gtcaaatacg aaccaaccgg
    19081 attgtcttta gcagctgaaa agatcccttc cgcctctttc tcagggcttg tcgtgaaagg
    19141 gggacggcga aatacatgtc cctcccacgg atacatcgta ttgatgtaat ggatcttgtc
    19201 ataatttgct aattcaatat gactaggaac agtgatttca tcaaaagaac aggcatcaaa
    19261 atctttttta taaaatgttt ttggtcgtac cgaaggattt ggcgaataac aaaaacgcca
    19321 tgatccattt aaagactgtt taagggagga tgtggtttct acttcttccc ttgttgcata
    19381 acaaacatga tcgctatgtg catttgattg gttcactcta aaaacttgtg gatcatctaa
    19441 ccattttata tttgctttca tacttttttc cttctctctc ataacttttt gaaatgaagc
    19501 agttctgttt ggaaatcacc ttgaaaatcg gggaagtaaa gtccaactgt catcaattcg
    19561 gcccccgaaa atatccgctt cgaggtctgt tcctgataaa gtgcctcatc atctaacccc
    19621 atcagatata cttcatggaa cgctggttga gctgaagcta gtatcctccc taaaaacaca
    19681 atagcttcat cttgtttagg agacacaaac atccatgcgg caatattttc ttcaaaagga
    19741 ctcgccaaac gataataatg gccatactgg atcaccggac gaatcgtttg ataggatata
    19801 atttggtctt ttacttgctt tttttcttca ggagacagct ttgtcaaatc cagttcataa
    19861 ccaaaaacag cgctcatagc tgtttctcct cttgttttca acgaagtaga tctgtcggtc
    19921 tgatgatttg gtacagctga tacatgagct gtgatggacg aaatcggata agcaaggctt
    19981 gttccatatt ggattttcat acgctccact gcatccgtgt tatcacttgt ccatgactgc
    20041 ggcatataat aaataaaccc ggcatcaaac cgtccacctc caccagaaca gccttcccat
    20101 aagatttccg gataatcagt tgtcaaatgt tcaagtagtt cataaagccc gagcatataa
    20161 cgatgcgcta cttctccttg tctctgtggg tcaagggtaa tcgaatagac atctgaaagc
    20221 gaacggttca tatcccattt gatgtagtca agaggaatcg tatctaaaat ctttctcatt
    20281 tgtttctcaa tcgtctgccg aacatctaac cttgtaaaat ccagtacatg ttgtgatctg
    20341 gaagcagaag gcattctacc tggctcctgc ataagaaaat caggatgtgt tcgatacagt
    20401 tcagaatcaa ccgagatcat ttcaggttca aaccataaac caaatttcaa tcctttgcta
    20461 tgagcataat ctgctatttc acgtaatcca tttgtcaact tgccttcata ctcgaaccag
    20521 tctcctaatg aagaattgtc cgcatctctt tttccaaacc agccatcatc taatacgaac
    20581 atttcgatgc ctagctcctt tgcttcatca atgatcgcct tgattttttc actattgaag
    20641 tcaaagtaag tggcttccca gttattgacc agaatcggcc gttcagcaaa ttgatgacga
    20701 cctcttgcga ttctttcacg tagcaactca tggtggatct gactcatttt gttcaatccc
    20761 tgacttgagt aagaaaggat catttcaggt gtttgaaaag actggcctgc attcaattcc
    20821 cacgaaaaac gatggctgtt gatcccaccg acgactctca attgattgat ctgatctttt
    20881 tcaagttcga aagaatggtt gccggaataa acaaattgaa gtccgatcgc ttctccagta
    20941 ttttctgtcg tatgatgatg aactaaagca ataaagttat tcatatggtg actgctcgtt
    21001 cctcgacggc tttcaaatac ttttgttcca gataaaacag attgccgtga aatctgtctt
    21061 tcattgacat gtgctcctgg caaagcaatg acttcatcaa atcgcctagt atgggcaaag
    21121 tctagctgga aactagccgc tttttctagt ttgattgttt ctccggtttc atttcttatc
    21181 tttacagatc gtgtgatcac tgctctatca gtaaatatgg tatagaacaa atcgaaataa
    21241 atcgctgttt ctctatcctt aagtgaaatc accaacgttt gtgcctctga cttgtccatc
    21301 acataagttt gaggcaagcc ttcgatctct ggcttcccat ctgtaatata atggctgtca
    21361 tatctcagat ccagtaagtc tgatccattc ttgcgctgaa tgatcgatgc gggcgttcga
    21421 aagtccatgc tgccatgaga agagtattct tgcggtagtg tatctttcga cagagaccgg
    21481 tcaggattta agggaacatt tcctgaaaat ccacgatctc ttcggggata atttttattg
    21541 tctttgtact gcttgactgg tttgccaaaa taaatatgcg ccaatacatt gctttcttct
    21601 atctttatca gataagaaag aaatgtattt cgtaaatgga agactcggct cttttcatca
    21661 aataagatga aatttttatc tgtttttcta tgcgtaatat ccatcatttc actcccaaca
    21721 aaacggaaac cgtttacttt tttttataat tcctgtatac tgtttactaa acaattttgt
    21781 caataaaaat tttttatttt tttgaggagt gtgtaaaaat ggttggaatt agagatgtcg
    21841 cacgaaaggc aaatgtctct cctgctacag tttcccgtgt gttgaataac gatcagacga
    21901 tccatgtggc tcagactact cggcaacgga tagtagaggc agctgctgcg ttggattatg
    21961 acatcagtac tcgtaaatat atcaaaaaga gaatgccttc gattggtgtg atctctacga
    22021 tcggcaaagc gagcgaagaa caggatatct attacaaaca tttgcgagta gggatggaag
    22081 aagaagctcg acgtctgcat ttagggatga atcgaatcta taatctatct gataatccta
    22141 aacaatggga agatctagat cagttaggtg cggtgatcgt tgttgggact gttacgaaag
    22201 aatcgattac cgccttgtta aaacaaaaca tgcaaatagt cgtcgtggat aaccctgaca
    22261 tccagcaaga ggtcgatatg gtgtatgcag atcttgaacg gatgactaaa accgtactgg
    22321 aattgttctt agaaaatggt cataaaagaa tagcctatat cgggggatat aaagtcgata
    22381 tggatgaatt cggtgggaag cagatttcta taaatgaaaa gaggatgcgt gcctatcagc
    22441 attttatgat cgatcatcac ttagaaaaat atatgtctta tcatctaggt ggctggacag
    22501 aaaaacaagg tgaggtactt gcgactaatc tattagaaaa tgaatcccct cttcctactg
    22561 ccattcttgt cgggagcgat ccaatggcaa ttggtgtcta tcgggcattg aaacaaaaaa
    22621 atatccaaat tggagaagag attgttgtag caagctttga taatatcgag acaatcgcaa
    22681 acctctcccc tggcttgacc agtgttcaaa tcaatgcgaa atcaattggg caagcagctg
    22741 ttcgaatggc attggagcga attgacggta tacgggacga accaatcatt ttgacttatc
    22801 ctgcacgatt gattgttagg gaaagtttta tcccaagaac aaaaaggtat tgaaagagag
    22861 gttgtgaaaa gccgcttcgt ttcttcatac tcaaaacgaa acggcttttt cacaacctct
    22921 tacttatact tattcacaaa atccaatgtc tgctggtaac aattttgcga ttcttttagc
    22981 gtgtaatcaa attggtattc atgggtaatc tctttttttg tatctgtata aaacaaggat
    23041 tgcacgggta cctttaattc tgtcaaccga ttttctaatg cttgaccttg ctcctggaat
    23101 gagtatgcat ttccgtcagt tatatacgtt ggcggaaatt ccttagaaac atggtcagcc
    23161 acactagctt ctttcaattg ctcactcgtt ttccaatctt ttgttccaat caacgaccac
    23221 gcaacggttt tgacgaaaaa cttcataaat cgatcatccg aatgctgctg tgctgttagc
    23281 ttcaaatcaa caggtccgca ataagaaatg gttcctttca gactatcttt tggaagtagc
    23341 tgtttgattt tcatttgttt agcatacgca tcattcgttt gtatagctgc atattgcaat
    23401 gcgatttgtg aacctgcact atctccacca aaaaataatt gttccatgtc tagcaatttg
    23461 tcgtcttttt cttcagctaa taaatctttg atcagttcat tcacttgaag aacttgatta
    23521 gggtattctg aatcaggcgc taattcataa ttcatcgcaa ccactgcgat gttggcgtct
    23581 gaaactaatt ttgtcgcaaa ttctttgaca cccgatttat ctccgccgac gaatcctcca
    23641 ccatgtaccc aaaatagaac aggaacaggc cccttgctgt tttttggata gtagatgtca
    23701 taagtattct ctttatactt ggactcatac gtcaaatctg tttttttcgc cacgtttttt
    23761 tctgcttgat cgaacgtttt tttatcagtg atttgaacag tatttgaaaa catatggttg
    23821 atcaagaatg ctccaggtct gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc
    23881 ccaagaatga ttccgaagaa ccatccgata aatttaagaa ttttcttttt cataaggctc
    23941 cctcagtagt tagtattaag cattctgatt ataccatagt gttatttcct atcaagctct
    24001 cctagatgat ttaatgattt tccccaaact tcttctgcaa tgcttttgac aaaagaaatt
    24061 ttttccattg ttcttcaaac aaaacatttt cttcttctgt tgaagcaaat ccacattgag
    24121 gacttaagca caaacgatcc aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca
    24181 cttgctggtg ctcttccatt tcgccatttt tcgttacagt caaaatctaa gacaactaat
    24241 ttatcttcag agactttctc taaaagaaca aaactcccag cacgttcact atcgtattct
    24301 aaataatagg cacggacatt ttcttgagta aaataatggg tctgctacgt cgtcatcccc
    24361 cctgatgccg ccaaaatcga ataataattt ccgcgtcaaa catgtatatt tagtgttgaa
    24421 tcatttgaga atatgaaaat aaaaatcttt ttgatcaatc agttaatcaa attgaaataa
    24481 cgaaaaatct gagacagaat gacgttcatt ctgacccaga tttgattttg cacaactcaa
    24541 cagtccttat tggcaaatcg ccaaaataat gtagactaac t
//
LOCUS       DAPBCS010000046        23674 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPBCS010000046 DAPBCS010000000
VERSION     DAPBCS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.047"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            288..695
                     /locus_tag="RD521_002082"
     CDS             288..695
                     /locus_tag="RD521_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771789.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="RD521_002083"
     CDS             709..1110
                     /locus_tag="RD521_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771790.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="RD521_002084"
     CDS             1112..1483
                     /locus_tag="RD521_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771791.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1821)
                     /locus_tag="RD521_002085"
     CDS             complement(1519..1821)
                     /locus_tag="RD521_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771792.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2070..2270)
                     /locus_tag="RD521_002086"
     CDS             complement(2070..2270)
                     /locus_tag="RD521_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDT7771793.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2575..3807)
                     /locus_tag="RD521_002087"
     CDS             complement(2575..3807)
                     /locus_tag="RD521_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDT7771794.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4062..4631
                     /locus_tag="RD521_002088"
     CDS             4062..4631
                     /locus_tag="RD521_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7771795.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4809..5249)
                     /locus_tag="RD521_002089"
     CDS             complement(4809..5249)
                     /locus_tag="RD521_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDT7771796.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5407..6171
                     /gene="map"
                     /locus_tag="RD521_002090"
     CDS             5407..6171
                     /gene="map"
                     /locus_tag="RD521_002090"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDT7771797.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6203..7126
                     /locus_tag="RD521_002091"
     CDS             6203..7126
                     /locus_tag="RD521_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDT7771798.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7202..8341
                     /locus_tag="RD521_002092"
     CDS             7202..8341
                     /locus_tag="RD521_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDT7771799.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8334..9134
                     /locus_tag="RD521_002093"
     CDS             8334..9134
                     /locus_tag="RD521_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7771800.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9134..9961
                     /locus_tag="RD521_002094"
     CDS             9134..9961
                     /locus_tag="RD521_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7771801.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9939..10673
                     /locus_tag="RD521_002095"
     CDS             9939..10673
                     /locus_tag="RD521_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7771802.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10773..11639
                     /gene="rfbA"
                     /locus_tag="RD521_002096"
     CDS             10773..11639
                     /gene="rfbA"
                     /locus_tag="RD521_002096"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDT7771803.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11653..12225
                     /gene="rfbC"
                     /locus_tag="RD521_002097"
     CDS             11653..12225
                     /gene="rfbC"
                     /locus_tag="RD521_002097"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDT7771804.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12247..13275
                     /gene="rfbB"
                     /locus_tag="RD521_002098"
     CDS             12247..13275
                     /gene="rfbB"
                     /locus_tag="RD521_002098"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDT7771805.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13373..14224
                     /gene="rfbD"
                     /locus_tag="RD521_002099"
     CDS             13373..14224
                     /gene="rfbD"
                     /locus_tag="RD521_002099"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDT7771806.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="RD521_002100"
     CDS             14259..16292
                     /locus_tag="RD521_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771807.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..17616
                     /locus_tag="RD521_002101"
     CDS             16336..17616
                     /locus_tag="RD521_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDT7771808.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYRWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17826..18632
                     /locus_tag="RD521_002102"
     CDS             17826..18632
                     /locus_tag="RD521_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771809.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18644..19864
                     /locus_tag="RD521_002103"
     CDS             18644..19864
                     /locus_tag="RD521_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771810.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19854..21440
                     /locus_tag="RD521_002104"
     CDS             19854..21440
                     /locus_tag="RD521_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7771811.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            21479..23617
                     /locus_tag="RD521_002105"
     CDS             21479..23617
                     /locus_tag="RD521_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7771812.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         7569 a         3940 c         4853 g         7312 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtttttcct tccatcagca taaaagttca cctcaaaaag agtatacgaa cgaatgttct
     1861 ttttgtgaag gcggaattta atatactgaa taatagaaaa caagtacttg tgccaaatta
     1921 tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat agaaaacctt ctatattaag
     1981 atttcctgca ataacaaaca caacagaaga cgtgtaatag ttagtcagga acgtacaaat
     2041 aacccctgta tcctttgcgg tacagggggt tattttgtat aagtagacat tgtagtttct
     2101 aaactgctat gtcctaaacg tttcgatacg ctaagtatat ttactccttg ataaagtaaa
     2161 atagatgcat gcgtatgtct tagtccatca ataaacagtc gaagagaaaa tagggaaaaa
     2221 ttgattacta aagaaaaact tactaggtcg acggtgtagt tgattttcat acttcaatag
     2281 attcgaatta aacgatttcg attggcttat accagtccta tccaacgaaa gatatatcgt
     2341 tcttaactct agcggtataa cttgaagctt tacgtaaagc caatgggaat gaactggaga
     2401 tgacttttta ttatttttag taatagctga tgggttaata cttacaactt tccttttcaa
     2461 agtaccatat aaaaaaacgg tggggttgtt tagcgaaata aaggatctaa gccaatagct
     2521 tagatccttt attctatgct ttatattatt ttttgcatgt gtgcgaaagt atactcaagc
     2581 ccaatcgcca tttcggaaaa tcggtactgt agagccatct tcacggattc cgtcgatatc
     2641 catcttgtca gatcctacca tgaaatctac gtgggtttga cttctattca atccagcttc
     2701 tgctaattct tcatcggaca tttctgtacc gcctttcaca ctgaaggcat aagcagaacc
     2761 tagtgctaag tgattcgatg cgttttcatc aaataatgta ttgaaaaaga tgatgccaga
     2821 ttgagaaata ggtgaagggt caggtacaag tgcgacttct cctaaacgac gcgcaccttc
     2881 atctgtatca agcagttttg ctaagacatc ttctccttgt tcggcagaaa aatcaacaac
     2941 ttttccatct ttaaaagtaa atttcatacc cgagatgatg gtgccagcat aactcagtgg
     3001 ctttgtactt gaaatgtaac catctactcg atgactgtca ggtgctgtga acacttcttc
     3061 cgtcggcata tttgccatga atttttctcc acgagcatta tagcttccag cgccttccca
     3121 aagatggttt ttaggcaatc cgataatgat atctgtaccg ggagcagtat agtgaagggc
     3181 agaaaattgt tctcgattca attcttctgc tttttttgct aatttttcat catgcttctt
     3241 ccatgctaag acagggtctt cttcatagac acgagtcgtt ttgaaaatct gatcccataa
     3301 tgcctcaact tgtttttctt ctggcagttc tgggaagact tttgctgccc attgtttacc
     3361 agccgcagcc acgacagtcc aactgacttt atttgcttga gttgccttgc gtaaattcat
     3421 tagtgctttg ccattcgctg attgataaga cgctactcga tggctatcta caccagcaaa
     3481 tgcatcggga tcggcagaaa caacgctgat cctgcttgct cctttttcaa gccattcatc
     3541 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
     3601 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
     3661 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
     3721 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
     3781 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
     3841 actattagca tgataccatt taattatgat tcaaacaatg aggctttaaa taaaaaaagg
     3901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     3961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga
     4081 ttactagaga acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac
     4141 gatttactgc gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg
     4201 agtttaaaaa tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac
     4261 aatatgaagt gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt
     4321 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
     4381 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
     4441 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
     4501 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
     4561 agattgaaaa gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg
     4621 aaatcgattg aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa
     4681 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
     4741 atgaatgcaa tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg
     4801 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
     4861 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
     4921 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
     4981 gatccgcaga caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag
     5041 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
     5101 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
     5161 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
     5221 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
     5281 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa
     5341 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
     5401 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
     5461 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
     5521 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
     5581 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
     5641 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
     5701 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
     5761 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
     5821 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
     5881 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
     5941 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
     6001 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
     6061 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
     6121 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
     6181 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
     6241 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
     6301 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
     6361 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
     6421 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
     6481 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
     6541 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
     6601 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
     6661 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
     6721 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
     6781 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
     6841 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
     6901 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
     6961 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
     7021 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
     7081 atggttcaat aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt
     7141 attgtaaatt tatatcagta atttataata attatgactc ttaattaaag taggtgccca
     7201 tatgcttgtt tcttttagta tggttataag attttttctg acaattttat tatcactgat
     7261 attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga
     7321 aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt
     7381 tgttatatcg actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat
     7441 cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac
     7501 acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga
     7561 tatacgtatt gattccttca cgcttccttt tattggatat atccaactag gctggcttag
     7621 ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg
     7681 tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg
     7741 atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc
     7801 aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac
     7861 aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt
     7921 gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt
     7981 ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca
     8041 tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta
     8101 tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg
     8161 aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg
     8221 tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga
     8281 gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata
     8341 acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc
     8401 ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt
     8461 ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct
     8521 acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc
     8581 tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag
     8641 agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac
     8701 ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt
     8761 ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac
     8821 tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct
     8881 caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc
     8941 gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca
     9001 agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa
     9061 atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt
     9121 ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt
     9181 cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct
     9241 tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat
     9301 cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc
     9361 tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga
     9421 cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc
     9481 gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg
     9541 tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc
     9601 aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc
     9661 tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga
     9721 gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag
     9781 taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag
     9841 gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt
     9901 caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg
     9961 aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt
    10021 tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt
    10081 cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc
    10141 ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga
    10201 aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa
    10261 agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca
    10321 ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag
    10381 aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt
    10441 cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga
    10501 ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa
    10561 gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt
    10621 ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga
    10681 tgtttaaatc cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg
    10741 aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca
    10801 gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg
    10861 acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc
    10921 tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg
    10981 atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt
    11041 ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata
    11101 tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg
    11161 cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg
    11221 agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg
    11281 taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt
    11341 cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac
    11401 tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat
    11461 tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt
    11521 gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg
    11581 cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag
    11641 gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg
    11701 gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca
    11761 gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt
    11821 gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta
    11881 gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt
    11941 caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga
    12001 tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat
    12061 tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat
    12121 tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta
    12181 aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat
    12241 tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt
    12301 cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat
    12361 gcaggaaaca aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga
    12421 gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat
    12481 tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg
    12541 aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat
    12601 cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat
    12661 ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat
    12721 tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta
    12781 cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt
    12841 attcctcgcc aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga
    12901 aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg
    12961 acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag
    13021 accgtgatgg aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta
    13081 aacgaccgcg cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag
    13141 ctaggttggc agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg
    13201 tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc
    13261 aaaaatggac aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc
    13321 aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt
    13381 attaactgga ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa
    13441 tctgccatat gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat
    13501 ggcttatatt acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt
    13561 agataaagca gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag
    13621 aaatgtagca cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta
    13681 tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact
    13741 aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga
    13801 ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac
    13861 gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg
    13921 accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc
    13981 atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga
    14041 aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa
    14101 agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt
    14161 cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa
    14221 gtaatttcct ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agaggaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtacct gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgtcg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaagaaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagact tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt
    16921 gggatagcag tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg
    17101 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    17161 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    17221 cagcgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    17281 ggattgatag gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg
    17341 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    17401 atgcagtcat ttatggagga tacattgact tcataccgat gggttccgat tatttatatc
    17461 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttacggagga attctatgtt
    17521 atggaagagg catatgacga tatgacttat gatgatacat cttatgagga acaaccagat
    17581 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct
    17641 gtgacaaccg tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata
    17701 gaaacgttag agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg
    17761 gtaccaaaaa tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa
    17821 cacaaatgtt taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattac
    17881 tgctacagtt ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct
    17941 tatgggcgtt tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg
    18001 gactgaaagc acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga
    18061 tgattccctg gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat
    18121 atacttattt agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc
    18181 tatccaatct gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa
    18241 atggtatata tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg
    18301 ctttcttgac aggattgact tggctgacgg catcgatcca accattttta cctgatatca
    18361 tgcaattaat tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc
    18421 ctaatcagtt tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg
    18481 ttcaaggtta tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata
    18541 ctttgtattt ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta
    18601 gattgaaacc acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat
    18661 tagactaaaa gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg
    18721 tgaagccctc aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt
    18781 gaatgtagaa ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa
    18841 atctacaatg ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat
    18901 cgaaggaaaa atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg
    18961 ctatgaaaat atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga
    19021 aatggtcgat gatatcattg aatttgctga catcggcgac catatttatc aacctgtaaa
    19081 aacctattca agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc
    19141 tgatattttg attgtggatg aagcattggc agtaggagat cttgaatttc aactaaaatg
    19201 tatggaaaaa tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga
    19261 tattagttcg attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gaaaagttgt
    19321 cgaatatggt gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc
    19381 agttaaaaca gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt
    19441 agacgtgacg aaagccgaat tgttggataa agataaaaat ccagtggata tgctggaaca
    19501 aggagaaaaa ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc
    19561 agttttagga attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct
    19621 actagatggt atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga
    19681 aaagttaggt ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc
    19741 aacggttcct ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat
    19801 cggagaaggc atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat
    19861 atgattttga aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca
    19921 aaccaggaag taaagtttta gagtttggtc ctggaaacgg ccgattaacg aaacatttga
    19981 tcggagcaaa acaatgtgaa gtgagcattg tcgagctaga taaggaattg ttcgattttg
    20041 tcagcgagtt tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact
    20101 attatgctgg acaaacattt gattacgttt tgtttgccga tgttttagaa catttggtag
    20161 atcctggaaa aacgttgaag aaagttagag aatttttgaa tgaagaagga gaaattctaa
    20221 ttacattccc taaccttgcg cataattctg tcatgattga tttgttcaat aatcaattgc
    20281 catgggcttc ttatggtttg ctagatgaaa cccataattc tttttatact catgatggct
    20341 tccaaaaagt ttttgaaaaa gcaggattat acatcaatat tgaagattat ttgtacttag
    20401 ctgtaggtga tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgttatg
    20461 atttcaaaat gcgaccattt ggcgaagtct accaatactt cttctcattg atgaaacatc
    20521 cagtagcaca aagttccatt gcgcagccgc aaaattctaa ttatgtgaaa gtattagaag
    20581 taatccagca aacgaaacaa gatgaaataa ttcaacaaat accattcaat aatttcacag
    20641 gggaaaatga gacactaagt ttccatgtat cagaattagc agaacgaatg gtgtttcgct
    20701 tcgcgcagca acctagcttt atcgaattca gcgcagaagc tgctggagaa aaactcacat
    20761 ttatcgattc aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatggtaaag
    20821 atttaccaga gttcttttta acggatattg cagggaaaga agtgactatc cattgtcact
    20881 atcgttttat cggagaattg acaccaacaa tgaaagaatt gctggaaaca atccgtccaa
    20941 tggctgaagt tacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcatt
    21001 atcttattga gaataataca agattagatc agacgttgaa aacaaccata aatcgctatt
    21061 gcacgttgtt ggaaagtgat gaatggacaa tcaaacatag attagggcga cgtaagaaag
    21121 aaacatcgaa aaaaatccaa gaaaaagagc tttctctatg tattgacgaa aaaatctggg
    21181 atgcagaaac aaagatattg aaaatcatcg ggtggggaat cgctaattcg gatcgccaac
    21241 cattgtccta taagttatca gccgaccaat caccattttt tgaagcaatt cctgttttac
    21301 gagaagaagt caatcaagca aaacagctag ctgaaggagc aaaagcagga tttgaactgc
    21361 ggatcctttg cgagaaggaa cgttcattct tagttgaagc agtggctcaa aatggtcaat
    21421 catggatcat tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt
    21481 gcaaaaaaaa gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga
    21541 tttgcttatt gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt
    21601 agaaaaagaa aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt
    21661 atataatctt gatgttaaga cccaaagcgg ttttaaggta cgtttatcag ggaaaatgag
    21721 aggaaaagca atcttagact tccaaacagc aagacatcaa aatggcatag ctgtgaaatt
    21781 aaacggtaaa tatccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt
    21841 actgaaaaaa gggataaatt atgcaaggac tcatggcgta aaaaaagcaa tccgccgggt
    21901 caaactggaa ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa
    21961 acctgatctg aaagagcaaa ggaaagaagt acaaggattt gcctaccgcc cattgatttc
    22021 catacttatg cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt
    22081 acttgatcaa acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc
    22141 agctatccga aagtgtctag aatcctatca agcaaaagat gatagaatta aagtcgtttt
    22201 ccgacaagaa aatggacaca tcagtctggc aactaattct gcattggaaa tggcagaagg
    22261 agagtttata gcgctcttag ataatgatga cgaattacct ccatttgcct tatatgaagt
    22321 ggcaaaagtg ctaaacgtac atccagaact ggatttgatc tacagtgacg aagataaaat
    22381 cgatgcagat gggaatcgct ttgacccgca cttcaaagca gactggtctc ccgatacatt
    22441 gatgggaaat aattatatct ctcacttagg cgtatatcgg acaagcattg ttaaggaact
    22501 tggcggtttc cgtaaaggat atgaaggctc tcaagattat gatttggttc ttcgtgtgac
    22561 agaacaaatt cctgcagaac acatttacca cattgaccgt gtcttgtatc attggcggac
    22621 gatcccggga tcaactgcaa gcaatggaga agctaaaagc tacatctatg attctggagt
    22681 gaaagcattg acagatgctc tatctagaag aaatatcaag ggaagcgtac atcctggcag
    22741 aatttctggt ttttatgaaa tcacttatga tgttttgcaa gaagacttgg tcagtgtgat
    22801 cattccaact aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa
    22861 aacgtcttat ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat
    22921 gcaagaatta tttgcagaat acaagcatca gctgaaagac cgatttatcg tcgaattaat
    22981 tgacattcca ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa
    23041 atacttttta ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat
    23101 ggtttcttat gcacagtttg atcgaatcgg ctgtgtagga gcaaaattat tttatccgga
    23161 tgatacaacg cagcatgcag gtgtattatt agggatcggt ggcgttgcag gacatgcatt
    23221 gaataactat gatcgaacac attgtggtta ctttggccga ttggtcattg acgtcaatta
    23281 cttagcagtc actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg
    23341 atttgatgaa atattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga
    23401 actgggtcgt tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc
    23461 aagaggttat gaagatacac cagaaaaaca aagacgtttt gctggggaaa tcaaaaaaat
    23521 gcaagataag tggccttctt atattgctca tgacccattt tataacgata atctaactaa
    23581 agaaggaatc ggcgattttt cgttgagacc tgattgatta aaagaactgt attttcattt
    23641 ttattaatga aaatacagtt cttttaattt aatt
//
LOCUS       DAPBCS010000047        22920 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPBCS010000047 DAPBCS010000000
VERSION     DAPBCS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.048"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..110
                     /locus_tag="RD521_002106"
     CDS             <1..110
                     /locus_tag="RD521_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDT7771813.1"
                     /translation="EVSMADTDFIAKLKFYFGFGYREKASATFGKSFKN"
     gene            688..2271
                     /locus_tag="RD521_002107"
     CDS             688..2271
                     /locus_tag="RD521_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7771814.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2708..3892)
                     /locus_tag="RD521_002108"
     CDS             complement(2708..3892)
                     /locus_tag="RD521_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDT7771815.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(3917..4924)
                     /locus_tag="RD521_002109"
     CDS             complement(3917..4924)
                     /locus_tag="RD521_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7771816.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(5096..5455)
                     /locus_tag="RD521_002110"
     CDS             complement(5096..5455)
                     /locus_tag="RD521_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771817.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(5427..5798)
                     /locus_tag="RD521_002111"
     CDS             complement(5427..5798)
                     /locus_tag="RD521_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDT7771818.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(5815..6117)
                     /locus_tag="RD521_002112"
     CDS             complement(5815..6117)
                     /locus_tag="RD521_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771819.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(6134..6604)
                     /locus_tag="RD521_002113"
     CDS             complement(6134..6604)
                     /locus_tag="RD521_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDT7771820.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(6579..6857)
                     /locus_tag="RD521_002114"
     CDS             complement(6579..6857)
                     /locus_tag="RD521_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDT7771821.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(6854..7903)
                     /locus_tag="RD521_002115"
     CDS             complement(6854..7903)
                     /locus_tag="RD521_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDT7771822.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(7860..8789)
                     /gene="tadA"
                     /locus_tag="RD521_002116"
     CDS             complement(7860..8789)
                     /gene="tadA"
                     /locus_tag="RD521_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDT7771823.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8921..10675
                     /gene="ade"
                     /locus_tag="RD521_002117"
     CDS             8921..10675
                     /gene="ade"
                     /locus_tag="RD521_002117"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDT7771824.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(10773..11435)
                     /gene="trmB"
                     /locus_tag="RD521_002118"
     CDS             complement(10773..11435)
                     /gene="trmB"
                     /locus_tag="RD521_002118"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDT7771825.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11505..12287)
                     /locus_tag="RD521_002119"
     CDS             complement(11505..12287)
                     /locus_tag="RD521_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDT7771826.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(12491..13702)
                     /locus_tag="RD521_002120"
     CDS             complement(12491..13702)
                     /locus_tag="RD521_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771827.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(13695..14432)
                     /locus_tag="RD521_002121"
     CDS             complement(13695..14432)
                     /locus_tag="RD521_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771828.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            14570..15004
                     /locus_tag="RD521_002122"
     CDS             14570..15004
                     /locus_tag="RD521_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDT7771829.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            14997..15341
                     /locus_tag="RD521_002123"
     CDS             14997..15341
                     /locus_tag="RD521_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7771830.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            15521..16531
                     /locus_tag="RD521_002124"
     CDS             15521..16531
                     /locus_tag="RD521_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7771831.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(16600..17544)
                     /locus_tag="RD521_002125"
     CDS             complement(16600..17544)
                     /locus_tag="RD521_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7771832.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(17528..20248)
                     /locus_tag="RD521_002126"
     CDS             complement(17528..20248)
                     /locus_tag="RD521_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7771833.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(20245..21465)
                     /locus_tag="RD521_002127"
     CDS             complement(20245..21465)
                     /locus_tag="RD521_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDT7771834.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(21746..22591)
                     /locus_tag="RD521_002128"
     CDS             complement(21746..22591)
                     /locus_tag="RD521_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7771835.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         7054 a         4624 c         3892 g         7350 t
ORIGIN      
        1 atgaagtatc gatggcggat accgatttta tcgccaaact aaaattttat ttcgggttcg
       61 ggtaccgcga gaaggcaagt gctacattcg gtaaatcatt caagaattga tgaaaggcta
      121 ccgcttgcca ttgcaaccac aacgatactc ctgaacgcga tgttggtgag atttccaaag
      181 agaagagctg tttaatgcaa cggtattaag caaatggcaa aaggaagtca tgtagacaaa
      241 gttttttatc aaacaccata tgatttcgcc tgaacgctgg tttagctcag aatacaatga
      301 tgtggtccac gagacattac cctcagtcag ataaaaaaag ctctctgaca gaattacaca
      361 tcgataaata ttaagtaaat gtcagcgcta gttgaacttt ctgacacttt caggtacact
      421 ggaaaagaca actacacgaa ggagggaaaa tactatgatg cgcatgacta aaataacaag
      481 taatgaaaat taccaacgat accatatcgc atttagccat gcaattggtt ttacagttgc
      541 tgttgacggg acaagtttgt ccaaaacagt tgaattcacg taaaatcaat ggccatctca
      601 aggtattttt ctcatgataa tctgagttta aataacacag gccagggata accatggcct
      661 ttttgagtgg tctcaaggag agagatcatg atcatacaag caatttctat taaaaaaaca
      721 atcggcggga atccattatt tgaggattta tcctgtcaaa ttgaagctgg tgaaaaaatt
      781 gccttagtcg gtatcaacgg aactggcaaa tcaacctttt tacaaatctt gaccggcaga
      841 gaaggtgtcg acagcggtgt tatcagtcgc aaaaaaggct taaagatcgg gacggtcgaa
      901 caagaactta cagtttccga agcgaccgtc aaccactact tgctccattc tgccgctgag
      961 atacaggacc tcaaacagca aatgagtcgc tacgaagcat tgatgacaga accagataca
     1021 aacttagaga agtgtctggc ccgttacggt gagctgcagc atcgctttga agaactaggc
     1081 ggttacgtat tagaagaccg gattgcagcg atcttgcaag gcttagggat tccacaccgt
     1141 caggaagcac ggttaacaga acttagcgga ggcgaacgag ttaaagtcgc tttagccaaa
     1201 attttagcag ccgatgccga tttactttta ctcgatgaac cgaccaatca tttagatctt
     1261 aacagtattc gctggttgga aaactattta cagaatacaa aaaaatcggt tctggtcgtt
     1321 tctcatgatc ggcaatttct cgatcaagtc accacaaaaa catgggaact ggaagaaggt
     1381 gcattgattg agtatcccgg aaactacagc cgttttcgag tcttgaaaga agctcgcttg
     1441 gctgagctga caaaaaatta cgaactgcag caaaaagaag tccagcggtt gaaagtcatg
     1501 attcgtcgtt ttcgtcagtg ggcacatgaa ggagacaatg agtccttctt caaaaaagcc
     1561 aaagaactgg aacgccgcct ggcgaaactg acactcgtca aaccgccacc tcccccgaaa
     1621 aatcggctgc aaagcttgtc taatggtgga aaatctggca aagaagtttt catcattcaa
     1681 aacttgcacc agcagtacgc tgatcaagta ctattcaaag acagcagttt tgcagtctat
     1741 cgtggagatc atttagccat catcggcgac aatggtgcag gaaaatcaac cttactcaaa
     1801 ttattactcg gtgaggaaaa gccttacggt ggcaccatca aattaggtag tagcttgcaa
     1861 atcggttatt tgccgcagca gctgcagttt gccgatcccg atagccgttt gctggcttac
     1921 gccatcacat tgactggaaa cgaagaagtc gctcgacggc aattagccaa aagtggcttt
     1981 tatcaaagcg atgtgggtaa acggatcaaa gatttatccg gtggtgaaaa aatccgtttg
     2041 gcgttgctga aactcttttt agaaaaaatc aacgtcctga ttttggatga accaaccaat
     2101 cacctcgaca gctatgctcg agaagaaatt gaagaaatgc tgcaagacta tcaaggaacg
     2161 ctcctagcag tcagccatga ccgctacttt ttgcagcaac acttccaaga agcactcgtt
     2221 attgatcagg agcagatcag ccgccagccg ctcggccaac tgattgacta ggacgttttt
     2281 tcgcaacaaa aaacttgatt ttcatacctg tgctttcacg attcaggcaa agtacaggtg
     2341 gaaaatcaag tttttattat tcgatcaatg aatgtttcgg atcggttttc tagctaaaca
     2401 taaagcgaag aataaagtcg acagcccacg aggctgatct aaaaatgttt tttcttttta
     2461 catatgtaat gtcccttata aagatgaatg aaaataaaat attgggattt tcctgaaaat
     2521 caataaataa aatgccagtc ttttcccttt tgaccacttt tccaagcaaa attccctaag
     2581 aagatcctgt aaaaaagaag ggaaattgaa gttaaattac cattttgcgg tcttctccgc
     2641 aaaaaaaaca catgcaagaa ttccctcttg catgcgtttc atcttttatc ctaacgaatg
     2701 ctttggctta gccgcgcaga cgctccacat cacgggcaat cattagttcc tcatctgttg
     2761 ggatcactaa tactcttact tttgattctt ttgtagagat atctacttct tcaccacgca
     2821 attcattcaa cgctggatcg atttcacaac caaaccaaga catacctttg atgatttcac
     2881 tacgtacatg tccgtcattc tcgccgatac cagcagtaaa gacaattgca tcaacaccat
     2941 tcatcgttgt cacataaccg ccgatgtatt ttcggatgcg gtccacgaaa atgtcataag
     3001 ctaaacgaat atgttctttt tcatagttct tttcaagatc acgcatatca ctagacaatc
     3061 ctgttaggcc tagcagacca gatttcttgt tcaaaacatt gatcatttca tcaatatcaa
     3121 tctctaattt ttccattaaa tatgggagaa cagctggatc gatatcacct gaacgtgtac
     3181 ccattgtcac cccagctaat ggtgtgaatc ccatagaagt atccatggat ttacctccat
     3241 cgactgctgt gatcgataca ccatttccta agtggcaagt gatgattttt agttcttcga
     3301 tcggtttgtt caataatttt gccgcacgtt ccgacacata acgatgactt gtgccatgga
     3361 agccatattt tcttactttg aaatcttcat aataagacac cggtaagcta tataaataat
     3421 tttgctttgg cattgttgag tggaaagatg tatcgaatac agccacactc aaaatgtcag
     3481 gtaaaatctt tttaaacgct ttgatcccca ttaagtttgc aggattatgc aaaggagcaa
     3541 attctgctaa ttcagcaatg cggtccatta ctttctcatc gatcactact gaatccccat
     3601 aagtttctcc tccgtgaaca acacggtgcc ctactccggt gatttcatca taagagctta
     3661 aaatttgcag ttcaatcaac tgatctaaca gcatttttac tgctacttcg tggttttcga
     3721 tatccatgat ttgttcgaat ttttgattat cgccgtactt gatggtaaaa atagaatctt
     3781 gtaaaccgat acgttcgacg atccctttcg caactacggt ttcttctggc atttgatata
     3841 attgccattt caaacttgaa ctcccagcat tgattgcaat tgttttagac atatgactct
     3901 ccttctcgtt gaatgattac agagcagatg atttccagtt tttaaactcg ttgaaaaatt
     3961 ccgtcacctt ctctggttct tttaatgaag caagtttcac aagcaagact tcttttactt
     4021 gttgtgcttg tggtcctttt ttctgtaaga tcaaaatact tttttgcgat tgtttattac
     4081 ggaacaattc atctggcaat tgaatcattc cttgaagata accttcttct ttaaaccatt
     4141 ttttgatttc ttcactttga tccgtttcta aaaatccact cggcatcaaa aacaaaccaa
     4201 aaccatccgg cttcacatat ttcattgatt gctctagcaa taaatgatgt gcataactat
     4261 ggccttcttc ggtacttgtt aaaaactctt ttgctttttc gtcattcgga taataaccga
     4321 ttggcaaatc gctgattgca aaatcgacag gatctattaa taattcctgc aacccgtctt
     4381 gatgaaagta ttgaacattt gcttgtgtca aatcacttgt agaagctgcg acagccaaca
     4441 atgtatcatc aatatccaca ccaattcctt ctgtttggta ccccgcattc gataggttta
     4501 aaagaactgt taataacaag ttgcccattc ctgccgcaat atccagaatc ttaaccggtg
     4561 cttttttatt tgtgtatagt tgttcaataa gaaaaacaaa taaaaaaccg atactatcag
     4621 gagtgagttg atggttcgcc tgcaaatgtt ctgtctggct gcccttcaat aaaagtaatt
     4681 gagacagtct gcgccattct tctggctcga aagaaagttt cttcaactct tcataaagag
     4741 ctgtgatccg ttgcgttgtc tcttttgtcg gcactccatc caccacacgt acttgataat
     4801 catctattaa attttctgca ttttcgacat aggcttctaa aaaggaagtt cccaatgcat
     4861 tctgtaaaag ctgaatagct tccaggtttt gattaaaccc ttgttccatt ttttctggta
     4921 acatggtgca cctcatttct agatatatta taacaatttt ttcacctgtt taattctatc
     4981 gaacaattga caaaatttca atcgttttca ctgtttttta tttgtgaaac ggcaaacatt
     5041 tttttattaa aaacagtgtt ataacagaaa agagggcgtt ttcacgaaca ttccatcatt
     5101 ctgtactagt atctgtatca ttttttactt cttctgtgaa ccgaaagata aacggtgaga
     5161 cttccacctc taagaatagg gactgttgta cccactgata tgttacagat ccagttgtgt
     5221 agtaaaaaac tccttccgaa ctttcggcaa gcgtagactg ttccgataaa gccatctgct
     5281 tcattatttt tgctatataa taatttttga ttcgtatatt aagatccatt gacaaacggt
     5341 agctttctaa tgctgccatc aataaaaacg tacataaaag aaataaaagc aagacagaaa
     5401 ataaaataga gcccttataa cattttctac tgatcgtttt ttgtccatat ggcatatcca
     5461 tcttctccat tttcaaagtg tacataaaaa gaaattgtgt gttctttttc caataattga
     5521 agctcagtga tacttgttaa cataggttga tgtccgccgg aagttcttct tttacgtatc
     5581 attgcctgat atctttcgat gatatactgt ttatcctcga tcgtgtagtg cagttgctga
     5641 tttttcacag agacattgtg actgctcttg agttcttctt ctaattgtgc taaaaatatt
     5701 agccactctt tttgatcttt acgctgcaaa aattcactta cttggatggt ttgctgaatc
     5761 aaaagattga atgtgagcaa tagactgctc aatatcatca gtccaattag acattcaatc
     5821 agcgtaaatg ctggaattac ttgttttttc aatactgatc gcttctttct ccatattgac
     5881 aaacactttt tcgattactc cattattatc gacaatttgg atatcgtatg ggatcatttc
     5941 ttcccttcgc tctggtcttt tgtgatacat ccgctgcgtc ttgatatctt cataaatcat
     6001 tcgaataaat agcagttcct ccccactttt cttttctgcc tgaagcaaaa acagctggcc
     6061 aaagctcact aagtagacac agacagaagc gatcataagc gctgtaaggg actccaacaa
     6121 aacaaatcct gttttattca attctttttg tatagcgacc actccctatc tggaattgat
     6181 acgtaatcgt ccgctctttt tcttcccaat aaaattgata agcttttagt gaactttcat
     6241 tcccggttct agcagcaaaa gtaatagaag catggcgttt caaggtaatc tcatcaggaa
     6301 tatctaatac ttcccaattt attttatctg gattcggaac atgaaaaatg atttgattct
     6361 cttcactatt ccaataaaag cctgtttgga tctgattcac aatagcgatt ttttgcgtag
     6421 aatagattcg tttttcaaat tggccgaaaa attgatagac tgccaattca ttcttccatg
     6481 cagaaaatgc gagaactgga aaagaaagga tcgttgtaag taataaaatc agtaagagtg
     6541 attcaaacaa cgtatatcct gatgctaaaa ccttaccttc aaggccgctg atagatttct
     6601 gcatgttctc gtttgatata gccttcattc aacaattcct ccacagtagg aactcttcca
     6661 ttattttgaa gcgcatagag ctctttttgt gtgtctacca actggatgat cgcttcacga
     6721 ctttcttggt ctacatgatt cctataacgc gacaaattag ggacgaacaa caacactaat
     6781 acactgatga tcagtaagac gattagcatt tcgatcaatg taaaaccgct ataataagat
     6841 atttttctct ttttcatggc aaaacctcct ctatgcttcc gtaaacaggt aataaaacag
     6901 cggcataaac taataaaatc aaagccgcta cgcccaaaaa aatgattggc tgaataaaat
     6961 gcatccagtg atgaagacgt ttgaaaagct gttttcttgt ccattcgctg taaaaaatca
     7021 gttctttacc taaacttccc tttgcctctc cctgtaaaac aattttactg aattcttctg
     7081 atagaaaacg ataattctgg aactgctcag ccaatgacat acctgcctcc aatccttgaa
     7141 tcaacttaga ggcaagatgc tgaatcagac tttcttgttt cgtttccttt aagcagacta
     7201 tgatttgcct taattcgaaa ccttcatgaa aaagctttcc aatctctagt gacaaataag
     7261 ccgattgata caaggaatat atcggtccaa tcaataattg tctgcttatc cattcactct
     7321 gatcaacagg agacaacttc atcagacgat accttactag taaagccaat agagtaaccg
     7381 ctaggatgaa tagtagaaca taccaatgaa agacttgtat cagctgaata ccccaatgct
     7441 cttgcatgac cattcctgaa ccaataagct gaggcaaaac gaacaaccgc atactaaaca
     7501 agatacctag caaaaaggtg agcaatagca tcggatatgc aagcagcttt ttcaaatcat
     7561 tgcgaaatcc ttcaacaagc cgaaactgtt tggcaatttc caacaaagtt gtcggcagat
     7621 tcccatgacg ttcagctaac tcgatctgta ccagttgctc tgctccatat cctatttgcg
     7681 aaagtacctc actaaacgct tgcccttcct ttagctgatg ttggatattt gccaaaataa
     7741 caggtggaaa cgaacggatc gtgagtaata aataaaggga ttcttgcaga ctaaaaccag
     7801 ataaaagcag ttctcccatg gtttgtgcaa actggttttg ctggcttctt gttaatcctt
     7861 tagaatagtt ctgactgcca aatattttcc tgagcttctt cataactttc ctcccatgat
     7921 ttttttactg attcttcctc cataaattga taactccata aaacagaagg atggtgattg
     7981 gcatcttcca aaagttcttg ataaatgaca ccagctaaac attcagatag ctcttctttt
     8041 ttctttgtca gttcatggat tctgaccaat gtaccttcaa gattgcgtgc atgtacggtt
     8101 gcaaaaaccc ggtgccctgt tagtgctgct cttaccgcag catgtgcagt tgattcatct
     8161 cttatctcgc caatgattag caagtctgga cgatgccgta atgcgagttt caaaagtgta
     8221 tcataagtct gtgctatttt cggattgatc tgtagctgca gaaaaaacgg ttcttctatc
     8281 tctacaggat cttcgatagt aatcacttgc aatttttctt ccaaagccaa ccgatacatt
     8341 agagaagtct tgcctgatcc gactggtccg ctgaaaaggt ataatccacg tctgacggtt
     8401 ttctgtaaaa tagttgcaaa atctcgagaa ctaaagcaac gatggggtgt ttcaaaagta
     8461 ccgtataata gccgaatcac taaactctct cgttgaaggt aatctcctac agaagacaac
     8521 cgcagacgaa cttcctgttt ttctaaagaa taagtaaatg cacctaactg agcttttctt
     8581 ttttctccga tatccatctg tcccaaatac ttaaatcttg agatcagttg ctgtccatct
     8641 gccgaagaga ctttcttcca gaatgttcgt ttttttccat agcgaaaata gatgtaaaat
     8701 acttctgaag ctggcaatat atatacgtct tgggcatgtt tggcaaaact ttcacttatc
     8761 atttgatccg ataatcgttt tatttccatt attctcccct ccacaaaaaa catacgtctt
     8821 tttgatcttt ttgtttcaaa taaaatgaat ttacaaaaaa atattggcaa aagccgaatt
     8881 tatgttatac ttataaaaat ttatcttggg aggcactcca ttggaaacaa cattaaggaa
     8941 atatatcgaa gtagcaagta aaaaaaagcc tgctgaccta gtgatcaagc atgctaagat
     9001 cgtcaacgtc tttacgaaag aaatcatgga aaaagatgta gcaatctgcg aaggaatgat
     9061 cgtaggaatc ggtgaatacg aaggaaaaag cgtacataat gctaatgggc aatatctagt
     9121 gccaggattc atcgatggcc atgtgcatat cgaaagctca cttttatcac caagcgaatt
     9181 ttcaaaagta tccttgcttc atggagttac tactgtagtg acagatcccc atgaaatagg
     9241 aaacattgct gggagtacag gtcttgagtt tatgatagaa gatgcaagac aaacactcat
     9301 gaatatcttt tttatgctac cttcttgtgt acctgtaacc ccttttgaga ccaatggtgc
     9361 aaatttaaat gcaaaaaaat tagccccttt tttacaaaaa cccgaagttt taggcttagc
     9421 tgaagtcatg aactatcaag cagtagcaaa taatgaaaca gacatcctag aaaagatcca
     9481 attgatgcac cagcataaga aaaaaattga cgggcatgca gctggtatcg gcatggaaga
     9541 gttaaatgtt tatcctgcag caggtattcg gacggatcat gaagctacaa ctgcaaaaga
     9601 agctaaagaa cgtttggatc ttggtatgta tttaatggtt cgagaaggga ccgttgcaaa
     9661 agatctggca tcgctactac cggcgatcac acctgaaaat tcaagaagat gcttctttgt
     9721 caccgatgat aaactgatca atgatttagt aaaagaagga tcaatcgatc atatcgtccg
     9781 tcaagctatt gcacttggaa tcgatccatt acaagccatt cagatggcaa cattgaatgc
     9841 agcagaatgt tttgatttga aaacactagg tgcgattgcc cctgggtatc aagctgattt
     9901 cttcttaaca gacgatctta ccaagctgcc aattcatgaa gttttctata agggaacatt
     9961 agttgttcaa tctgggcaac tccaagacca tttatttgct gatcattcaa attcatatac
    10021 gtgttgtctg ccaaaactaa acgctcaacc attaaatcca cattcgttag agctgcttct
    10081 ttcttcttca caagcccatg tgatcgaaat tatcccgaat agtctagtga ctaaagattt
    10141 ggtagaaaaa gtggaccgac aaggcgaacg attcattcct tcaatcgaaa aagaccaatt
    10201 aaagattgcg gtgatcgagc gtcaccatca aacgggaaac attggattag gcatagtgaa
    10261 aggatttcag ctgaaacaag gagcgatagc gacaacgatt gcccatgact cgcataatct
    10321 agttgtcgtt gggacaaacg acgaggatat gctgtatgct gctaatcttt tgattcaaaa
    10381 aggtggcggg atgaccgttg tcaacaaaca gcaggaaata gcttgtgttt ctcttcctat
    10441 aggcggaatc atgacacagg aacctttttc gatcgtctat gaacagttga atgaattaat
    10501 aactaaagca catcaactag gagcagctaa aacctttgat ccttttttga cactttcttt
    10561 cttaacatta tctgtcatac ccgaattgaa gatcaccgac aaaggattat tttcattttc
    10621 tcgcttccaa ttgattccac cttgtggaaa aaccgatgaa ataaatacat tttaaatcaa
    10681 ataaatccca gaaaatacat tacatctgta atgttcattt tctgagattt atttatggat
    10741 attaatgttt ttatgaaagc agaaagttag tttcacagac ttttcttttt ggtcttatct
    10801 tgaaattgag cttctactcg ataaatacgt tgaccttttg aagaaaattt ttcttcatac
    10861 tccgtcataa tattgccttc aaattgactt tgatgtaaat ctaaccatac ctgtttgagg
    10921 atcatgccat attgagaaaa acttgccaga gaatactcaa acaatccttg attatctgtt
    10981 ttgaaatgga tttctccatt tggacgaaga atctgttcat ctacagcaag aaaagtctta
    11041 tatgtcaatc gccgtttttc atgacgtttt tttggccatg gatcggaaaa attcagatag
    11101 atttgatcta cttcgctgtc agcgaaatac tctgtcaacg ctgatccatc cacatgtaga
    11161 agtttcaggt taggaagcgg ttgttcgatc aacttgtcta gtgcaatcga aacaacactg
    11221 acctgcattt ctactccaat ataattgatt tcaggatggg cttttgccat tccagtgatg
    11281 aactggcctt tccccatacc aatctcaatg tggattggat gatcgttttc aaacaattca
    11341 ttccattttc ccttccacaa tgttggatca ctaattacaa aattcgggtg agccgctaac
    11401 atttccgctg ctcccggacg atttcggaca cgcattattt atctccattc ctttcctcta
    11461 attaaaaagg tctggccaaa tctccgcaga gtttgggcca aacctcagcc actgaaaatt
    11521 cgttttaatt gtaaaatttc ttcgttcatc cgtcgatgat cttcacgttt gcaatatctg
    11581 gtgatttctt ggagcaaatt catggccgca taccaatgaa atttttcaag catttcgtca
    11641 gtcgggcgta tgccataact gatcagccac tgattccagc tagacagtgg cacataattt
    11701 cctaaaatcg ttgcaatatc caccgcaggg tctgcaaaca tgattgaatc ccaatcgact
    11761 agatataaat aatttttaca tactaaccaa ttacgatggt ttacatctcc gtgtactact
    11821 gataagtttt ccttatctat tgacgggatc tgctcacgaa gaaaacgatg gacaatttgc
    11881 agaaattgat ttttctggag ttcttccggt aattcttttt catatttttg cagcatcttc
    11941 tccggcgtca tgacacgacc accgatacgg gtcagcatgg ttttaagaga atttgaatga
    12001 tgtaaatggt acaaaacatc aatcacgtcg tttctttgac caatctcatt aggagtcagc
    12061 aagcgacctt ccagccactc ttgtgctgtc aaagtatcgc cattccccgt tcgttttgtc
    12121 caaacaagtt ttggtgtaat tccttccctt gaaagagcag ccaacatcgg agtcgtgttt
    12181 cgtttaataa agactttgtc ctcatcacgc atgcctatgt atgtttttcc tgtgtcgcct
    12241 ttgatgggac gtaaccgcca ctcttggtct aactgaaact ccatcatgca ttctcccgtc
    12301 ctcttactct ttgttttgca gtacttatta ttctagtcgc ctgaacacta cacgtcaagc
    12361 acatatacgt tctttttcat ttaattgcca ttcaaaaaac cagcttttct caaaaaaaat
    12421 agaacgaaca gcattcttac tgtcaacgct ctatttttca ttctcatttt taccctacac
    12481 agctttgttt tcagtctgac atcttcttca aacgagttgg tacatatagt ttaacaaaga
    12541 gcatagtaac aatcaaataa gccgctagcg gaacccatcg ctgctgccca tctaatacga
    12601 ctgcagcaat caaaccaaaa ataaccgacg cgataattaa tacccatgtg agtagatact
    12661 gaatcgcctg cttcttctga ttcttatgga ttggataaag ctgaacgaga accatatagc
    12721 ggaactggtt cgctaaaggt aacagttgga aaccgatcaa atataaaaat aagaaaccga
    12781 taatcagact aaaataaaag tcgactactc cggcaatcaa taacccacct ataattacta
    12841 atcggaaata aagaccacta aaatcagaac ctcttgccaa tcttcttgca aacaagtaaa
    12901 gataagtgtt ttttgattct cttttaatat gagtcaatat aggatccaaa tagcgtcttc
    12961 ttttgatctt tgccgtaatt tcaggaacat ctgtgaataa attgataaat tgatagatct
    13021 gatgcaacct atgttcttct acatgcacca ttttctccca atccaatcct ttttcttgtt
    13081 gcttccacaa aaaccagtaa tatagacttg cctgtactaa tgcacctgct attcctgccc
    13141 atattagcca ataaaatgat agaaacaaaa agatcagaga agaaaaaaac cagcctaatt
    13201 tccaataacg aataacttgt tgatctagtc gaaacaattc tgctctcttg atttgcagat
    13261 gactgaattt caaactccat aatatgatca gatagacgaa aaacatcgaa aaggattgct
    13321 ccgtactaac caccactaat ggcatggcca atccgcatgc caatatcaaa aagatcaacg
    13381 gaaaaatgca agaatagcga aaagctcgtt ccaaataggt ttgcatttct ttttctttag
    13441 gaagtaaaaa aacaatatct gctgcctctg caaaagtagc catcttaccc acgaataaac
    13501 agagcatcca aaatacagag attatgagcc ctccaattgc aaacggtggc tgcagggttt
    13561 tcaaccaatt agaataatat aatcccacac cgccaatcaa gaaaagacaa actaaaacaa
    13621 aatgatcgtt caatacataa cgcatatatt tcatcatttt cttttgatgt cttgctaaac
    13681 gtttttgaaa aaaactactc atgtgcctgc tcctttgtta aagcaatata gatttcgtcc
    13741 aaagaagaat ccggcagctg aaattctttt ctcaacatct caagagaacc gtctgctcgt
    13801 atctctccgt tatgtaagac cacaaaacga tcacaatatt tttctgcagt agctaaaata
    13861 tgcgtagaca tcaaaatacc ggctccttct tttttcattt ctaccatcaa ttcaagcaat
    13921 gcattgatag ctagtggatc caagcctaaa aatggttcgt cgatgatata taaagaaggc
    13981 tcgatcaaaa aggcgcataa gatcatcact ttctgtttca ttccctttga aaaattagct
    14041 gggaaccagt caagacgatt agataaccga aaagtctcca acaacttttc tgctcttttc
    14101 attgctatat ccataggtat atcgtaggcc atcgcagtga cttcgatatg ttctctcaat
    14161 gtcaattctt catataaaga aggagtctct ggaatatacc caatttgttt gcgatattct
    14221 tccgtatttt tctttaagga tagatgattg atttctatcg ttccttttga agcttccagc
    14281 agaccgataa tgtgcttgat ggtcgtactt ttccctgctc cgttcagtcc aatcaaccct
    14341 actatctctc caggctgtac atcgaaactg atatctttta acacagggag atgtccgtac
    14401 ccccctgtga catgatcgat aattaaactc attctttttc ctccgccatt tatttttatt
    14461 tattatacca cgaagaaaat gacggtattt tgtttttttc cttacaatag ctttaactta
    14521 tggtaaagtt aagctgaagt atttattctt tatagaaagg atgttttata tggaaaactg
    14581 tattttttgc aaaatcatca atcaagaaat cccaagctat aaaatatatg aagacgacaa
    14641 ggtctatgct tttttagaca tctctcaagc aacgaaagga cacacattag ttgtgcctaa
    14701 acgtcatgta gcagatattt ttgaatatga tcccgaactt gcaggagagg ttttttcgcg
    14761 tgtgcctaag attgctcaag cattggaaaa agcttttcca gaaatgaaag gtcttaatat
    14821 cttgaataac aaccgtgaac tagcctatca atctgttttt cattcacata tccatttagt
    14881 ccctcgctac agcaaagagg atgatttttc tattcacttt gtcaatcatc aagatagtta
    14941 tggatcggaa gaactaaaag ctattcaaga aacaatcgta aagcaggtgt cctgcgatga
    15001 ttaaaaactt tttcaaagga ctgttatttg gagctgctgc tggcactgtc ggaggtctgt
    15061 tattggcgcc caggagcggg aatgaaacaa gaaagaaact gatcgaagaa ttggaagaag
    15121 caacaactct gacaaatgat ttaaataaca gcctgaatca ttttaaaagc gcattgatcg
    15181 aaacaaaaga aacagctgaa tctgtgattc ctgctttcca agaagcaatc aaaaaagata
    15241 tcgaagattt taaattccaa gccgaaccac gtatcacaca aatcaatgaa caagtagaaa
    15301 aactaaaagc tgatctgcca gagtatcctg aacaaatata ataatgatta ttttctcaac
    15361 agatttaact atataagtga gaaaagtttg cgtatttcct aggaatgtga tgaaattttg
    15421 aattttgttt gaaactacca cgacaacgtt tttaaaggaa atgggatatg gtatagttaa
    15481 tatataaaaa taataactaa ttacaggagt gcaaatttta atgaaaaaaa aatcaattat
    15541 tttggcagct actagtgcgt tggctgtttt aactctagca gcttgttcag gcgatactaa
    15601 taaagatatc gcaacgatga aaggcggaac gatcactgta tctgatttct atgatgaagc
    15661 aaaacttgaa tcatcgaacc aatccttagt acaacggatg atcatttata aagtattcaa
    15721 taacaaatat ggcgataaag taaccgataa gcaagtggat gcagaatacg acaaacaagc
    15781 gaaatcatta ggtgatactt ttgaatcaca attagaagct gctggatata cgaaagatac
    15841 ttataaagaa tatattcgta acaatttagc ttttgaagct ggcttgaaag ctcacgtaga
    15901 tattacagat gacgatttga aaactgcttg gaaatctttc catccagaag tagaagcaca
    15961 aatcatcaaa ttatcaagtg aagatgaagc aaaagatgtg aaaaaatcag cggatgatgg
    16021 agatgatttc tctaaattag ccaaagacaa atcaactgat acagagacaa aagaagatgg
    16081 cggtaaagtt aaattcgact caacgacaac aactattcct gcagaagtaa aagaagcagc
    16141 tttcaaattg aaagacggtg agatctccga tgttattact acaacaaatc ctacttctta
    16201 tgcgacagaa tactacgttg tcaaaatggt gaaaaaccaa aataaaggca atgacatgga
    16261 taaatacaaa gatcaactaa aagatatcgc tactgaaact aaactaagtg acaatgcatt
    16321 cacaactaaa gtaatcggtg aagaattaaa agatgcaaat gtaaaaatca aagatgatgc
    16381 ctttgaaaat gtgttgagtg cattcacaac aacttcatca agtacgaaag actcaagtga
    16441 aactacagct tcaacaaaga gttctgacac aaaatcaact gattcaacaa aagaatcttc
    16501 aactgaagaa acaactgatt catcaaaata aaagaaaaca aaaaaagcga agacttatgt
    16561 ctcgcttttt ttgttttgaa tgaaaaaaat aaatttttgc tataaatctt ttggtacata
    16621 gaaacttcgg ttgtccatac cgaaaatccg atcggtgtat tttcctggtt ctgtttgacg
    16681 aatcgaagtc aacatcatct gcatcgaagc atcgatattg tcgatttgat gtaatatttc
    16741 tgcttccatt attcttggac gtacaggtga gccatattct aataagccat ggtgtgctaa
    16801 aaccatatga cgtaatacaa gaacatcttc gtcagcttcg ctgattttca acgcaagaca
    16861 ggctttagtt atttcttcgt caatcaatac cagatgaccg atcaaatttc ctgcaagcgt
    16921 atattctgtc gacataggac cggaaagttc aatcaccttt cctaaatcat gcaaaatgat
    16981 accagcatac aacaatgctc gattcagctc aggatattca tcggcgattg atttccctaa
    17041 gtgaagcatt gtcaaagtat gataagctaa tccactggca aatgcatgat ggtttctttt
    17101 tgctgccgga aactcaaaaa attgtttttg atatttatta agtagatagc gaacaatacg
    17161 attccaatgc gcattggtga tctcaaacaa caattgattg atttcttctt ccatttcttc
    17221 tttcttcaaa ggggctcttt ccatgtataa actaggctct cctggctcat ctacccttgt
    17281 taagcgcata tgaagaatct ttacttgagg gtttccttga taaacctctc ttttaccgtt
    17341 tagaaaaacg actgtgccgg ctgtaaattt attgatttcc tcctccgaag catcccagta
    17401 tttaccatcg atagtacctg aagtatcttg gaaagtaaaa gcaataaatt tttttccgtt
    17461 tttagcgatt cttacatctg cacttttgat caggacaaat tgttcaaact gctcatcgat
    17521 tactaaatca cgaatttttt tcattatttg ctccttctaa ttgttttacg atttggttca
    17581 tctcatggta atagcttacc atttctttat cagaagaaaa acaaattatt tgatactttt
    17641 ctccaaattt agctaaaagt tcagctaatt gatactttcg ctgatgatca taatgaagcc
    17701 agccatcatc aatgatgatc gggcatatcg aacggttagc ttctaatgag aggtaagcaa
    17761 atctcatcgc cataatcagc tgatctttcg tacctgtaga taactcatag atgggaaatt
    17821 ctgtatatcc gtctgttaaa gtcagtgtat catttttcag taatatcttc tgataatttc
    17881 cttttgttaa aatacctaaa taatcacctg ctgctcttaa caactggggt aattgtcttt
    17941 cactcatctc tgttgaaaga tcacctaaga atgtcccggc gatttcaagt gctccccatt
    18001 cctccagtaa ttcctgtaat tttgcttttt gccggctagc ttgttgataa agttcgtcca
    18061 atgtgccgtc tctttgcaac tcttctactt gaagttggag tcgttgccga ttttcacttg
    18121 cttctttttc ttcttgctgg agttgggatt gcttttcttt cagttggcgc agtcttacag
    18181 ataatttctc ttttgtcagc tcttcaggaa atagtggagt aagcatttcc gataattcat
    18241 tgtatctttt catctgctgt tcggcttgtt tggctttttg taaaaagaca ggtacttccg
    18301 aaggataaga taagcctgct tgagtcaatt gttcgctgta ttcttgtatc catacttttt
    18361 gtttcttttt caattgattg atttcttgct tcaataaagt attttgctga tagcttctgg
    18421 caaatttcat ctgttccatc tcttgtataa aacgggaaag caataccatt ttttctgaaa
    18481 ttgtcttatc ttgtatcggt aaccattctt taacaaactc aaatttttgg tcgatttgtc
    18541 gtagtttttc ttgtatttcc tgttctcttt tatgtttccg ctcaaatacg ccatataatt
    18601 tctgataatc tatgacttcc tgtccatatt ctttcatgag tttgagcgta tccatctttc
    18661 ctaagtgccg atgatccgca aaacgatata cttcttgctg aagaatctgt ttttcttctc
    18721 cagttctctt ttgtttttct tctgcttcag ctaattgagc taaggctaga tcaagttgtc
    18781 ctagtttttc ttgccacatt ccttttatct cagagaaatc atttttagtt ttaatttttg
    18841 gttgcctttt gtagaagaaa tagccaatca tgcctgcacc aattagtaaa aggatcactt
    18901 tcaagggcat tggtacaaat atacctgcaa tgattattgc tccgccagcc atcagcatat
    18961 acggtttaga tgaagagact ggtgctatct ctgactgatt ttgtaataat gctggatagg
    19021 tctcttccaa gtcattcact tctgcttcaa tcatctcttg ctgttcttta gctatttgga
    19081 gacgcacaga aatctggctt tctagatcag caatccttct ttcttcagcc agaagatgct
    19141 tccattcttc ttcatataaa aattcttccg gtaacggatt tttctgccaa tgccaccttt
    19201 gttccaaaat cttcaattct gaatttgttt ctctagtctc aatttgtaat ctttgccatt
    19261 cttcgtttaa ttgttccgct gtaaattgaa gttgctggag atcttttagt tcctgctcct
    19321 gctctaaata aaaataatat ctggctgact gctgatccat cccgctatgc ttttccagtg
    19381 ttgcttctaa ttcttccaaa cgctcatttg tttgctgata ccgtttggag aaagactcca
    19441 gatctaaaag aagttgctca tcaggaagac ctgtagtgct gttttttttg agagattgcc
    19501 attcttcata taaagaaaaa ttaagttctt gctgtctggc taatgaaaat tgtttgttcg
    19561 cctcttttaa ttgtgccaat aactgctgct gttgcgactc gtattcctgt tcttgttgga
    19621 tcaacttgcc aaaataagct tcctgttctt gtttttgatg aattttttct tgcagctctt
    19681 tccattcatt taatttttga ttgattagta atttttggcc ttttttcttg aataattgtt
    19741 gtgctttctg ataatattct tgccgctttt ggaatacttg agaactgccc gtgatgccta
    19801 aagaaatcag cgcatcatgt agctcttttt ctctcaaatg atctagttcc atcaactgct
    19861 cttgctggaa agtaaatacc tgctggaata actctttcgt caaaggtgcg atcagttttt
    19921 ctagtaactc atcacttccc acttgatccc ccagccacac cttcgatttt ccgcgatttt
    19981 gctgcttgta gcgctcgaca gcaaattcac catatacagg atgtttaagc catattttgc
    20041 cgccataggc tgagccgtct tttggtgtat aatccttttt tttacggcct tttgaaggga
    20101 aaccaaataa tattgcttga ataaattgat aaatcgttga ttttcctgct tcgtttcccc
    20161 catagatcag ctgattgcct gaataaaagt caaaactttt ttgacgccat tttccaaaag
    20221 cagcaatctc gatccttaaa atcttcattc ctgatcctcc tcaaataaaa aatcttgatt
    20281 aagcagctgc ttggcttttt caagtgtttc ttcttgatat tccggcagtt cgctcaatat
    20341 tctagccgct tcttgatgac tatatacttc atttaaaatt tgttggaaat cttgaggtgt
    20401 ctgatagtat tgaaataact gttccatcaa tttagcagaa gcagcaagtg gggtcttcgt
    20461 ataaaaagta ttctctatga gagaaatacg ccaaataaag aaatcattta tcctatctga
    20521 gaattcctct aacaaatagt ctaatagctc tcctgattgt atccgttcca aaacatctgt
    20581 attgagatgg tcataatctt ttaattttat ctcaattaaa ctaaatccat cctctacttt
    20641 tgaaagctgc tgacgaatgt gaacaatgac ctcttttgtc gttcttgctt tcctcaatga
    20701 tatttccttt gtcttccaat agacctctgc tactttaatc ggatgtattt ggcaacttcc
    20761 tttacttaat tcaactagta gaatagatgt agaagcttgt tcctttttag tatgaccttg
    20821 aggcgcacct ggataaacaa ttgtttgctt ttcatttaag accgttggca catggatatg
    20881 ccctaaagcc caataatcgt atcccttttc ttgcatcttt gagggttgga aaggagcata
    20941 gtttcctttt tgagagacgc caggttcccc gtggtacaac ccaatatggt aatctgtgag
    21001 ttctctagaa ggaaattcca tcactttgtc tttttgaatc cacggttgac gataactaaa
    21061 tccactgaga gagacagttt ctccactttt tatcgttatt ttttttgtct ctaccgtttc
    21121 ggatgtaaat aaatggacat ttttaggaaa ggagaaccaa taccgctctt tttgatagaa
    21181 atcatgattt ccgaatatta taaatacagg tatatggttc ttttctaacc gtttcatctg
    21241 ttctgaaaaa tgtttctgta tttttaatga aggacgattt tgatgaaaat tatctcctgc
    21301 aagtaaaaca aaatcaacgt tatttattat tgcctgttca atgatatttg ataaaacggt
    21361 cagattcgtc ttcaatagtt tttcctgtac agttttatca agagttgtca gtccttcaaa
    21421 tgaccgatcc atatgtaaat ctgccgcatg gataaatttc accattaaaa atttcctcct
    21481 ttttttcttt tatcatacca ttttcaatgt tttttttcat caataaccga aaatttgttc
    21541 gtattatgca aagaaattac gataaatggc cgacttcaaa aaataagttt attcgaaaaa
    21601 aagattgtga catgattttt gtcacaatcc taaatatcta tgttatattc gcttcgtttg
    21661 acactgaaga agtactattt gtactggaat ttatgctcca tttttccgta tttagaattc
    21721 tatgttctat actaccttaa ttagtttata tggtaagaat tctttcaaat gcttgagata
    21781 ctccgtcttt atcatttgaa acagtgacat aatcagcttt tttcatgatt tcttgatgac
    21841 tatttcccat tgcaaaacta atatctgcac tttcaaacat agataagtca tttaaactat
    21901 ctccgatcgc gattctcaaa ggtttcttta aattgtagat tgattccaaa cgatcaaaca
    21961 ccacaccttt agatacattt tgaggtacga tttcaatatt tacaggtgcc gattcagtaa
    22021 cagacacatt tagcatcttt ttcaaatggt taaatatatt agctttcaat tgacgattag
    22081 gaataaaaat aaaaaatttg taaatgctga tagcatcctt ttctataatt tcctcaatat
    22141 tattataagg gattgaacaa atagattttt catactcaag ctcatagttt agctctttga
    22201 tagaaaattc actccctacc tcgtgaaaaa tttttttcat tttattttta taattctgag
    22261 attcataact tccgagattt gtatatattt tggttggaaa tttcgatagg acagaatcaa
    22321 ttttttttac atcattttta tcgaaggaag actcaaatat ctttttttct ccatcaaatc
    22381 caacagaacc atttaaccct actgctggta tccttaaacc agattttttg agaatattat
    22441 taatgtcttc tacttctctt ccagaacaaa tgaatggaaa acagtcttcc ggtaaatgtt
    22501 tcattgttct tgcatttttt tgactaatgc tctggtcaga attcaaaaga gtaccgtcta
    22561 aatctatacc aattagtttc atttcatcca tgtctcctat acgttgatta taaattcact
    22621 gcttattgta agtctaaatt tctcacaaaa catatactaa ttatacctaa tttaagtaag
    22681 tacaaacatc attttaagaa taggaaagga catttatata ctattctctg tatttttgat
    22741 ttataaataa atatgaattg aatccaaatc agctatatta ctattttata ataaccaact
    22801 ataaaaaacg aagttaggtg aaaaactaac ttcgatattc ataaaagact ctattagttc
    22861 aacttagcaa aagatttaaa caaaaaaaga tgaactattt ctagttcatc tttttgcctt
//
LOCUS       DAPBCS010000048        22440 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPBCS010000048 DAPBCS010000000
VERSION     DAPBCS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.049"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            144..542
                     /locus_tag="RD521_002129"
     CDS             144..542
                     /locus_tag="RD521_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDT7771836.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1221
                     /locus_tag="RD521_002130"
     CDS             526..1221
                     /locus_tag="RD521_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDT7771837.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1815..2702)
                     /locus_tag="RD521_002131"
     CDS             complement(1815..2702)
                     /locus_tag="RD521_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDT7771838.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEGAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2887..3726
                     /locus_tag="RD521_002132"
     CDS             2887..3726
                     /locus_tag="RD521_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7771839.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNRTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3922..4896
                     /gene="sgrA"
                     /locus_tag="RD521_002133"
     CDS             3922..4896
                     /gene="sgrA"
                     /locus_tag="RD521_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDT7771840.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5194..5550
                     /locus_tag="RD521_002134"
     CDS             5194..5550
                     /locus_tag="RD521_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771841.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5553..6710
                     /locus_tag="RD521_002135"
     CDS             5553..6710
                     /locus_tag="RD521_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771842.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6736..7176)
                     /locus_tag="RD521_002136"
     CDS             complement(6736..7176)
                     /locus_tag="RD521_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDT7771843.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7336..7842
                     /locus_tag="RD521_002137"
     CDS             7336..7842
                     /locus_tag="RD521_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDT7771844.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8010..9923
                     /locus_tag="RD521_002138"
     CDS             8010..9923
                     /locus_tag="RD521_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDT7771845.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9928..10929
                     /locus_tag="RD521_002139"
     CDS             9928..10929
                     /locus_tag="RD521_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7771846.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11131..11337)
                     /locus_tag="RD521_002140"
     CDS             complement(11131..11337)
                     /locus_tag="RD521_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDT7771847.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11983..12831)
                     /locus_tag="RD521_002141"
     CDS             complement(11983..12831)
                     /locus_tag="RD521_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7771848.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12830..13897
                     /gene="gnd"
                     /locus_tag="RD521_002142"
     CDS             12830..13897
                     /gene="gnd"
                     /locus_tag="RD521_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDT7771849.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKRTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13899..14900
                     /locus_tag="RD521_002143"
     CDS             13899..14900
                     /locus_tag="RD521_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDT7771850.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14897..15181
                     /locus_tag="RD521_002144"
     CDS             14897..15181
                     /locus_tag="RD521_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDT7771851.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15214..16011
                     /locus_tag="RD521_002145"
     CDS             15214..16011
                     /locus_tag="RD521_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7771852.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16025..16858
                     /locus_tag="RD521_002146"
     CDS             16025..16858
                     /locus_tag="RD521_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7771853.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16876..17733
                     /locus_tag="RD521_002147"
     CDS             16876..17733
                     /locus_tag="RD521_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7771854.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17755..18264
                     /locus_tag="RD521_002148"
     CDS             17755..18264
                     /locus_tag="RD521_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7771855.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18301..18702
                     /locus_tag="RD521_002149"
     CDS             18301..18702
                     /locus_tag="RD521_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771856.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18814..19167
                     /locus_tag="RD521_002150"
     CDS             18814..19167
                     /locus_tag="RD521_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771857.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19201..19644
                     /locus_tag="RD521_002151"
     CDS             19201..19644
                     /locus_tag="RD521_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7771858.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19661..20746
                     /gene="uxuA"
                     /locus_tag="RD521_002152"
     CDS             19661..20746
                     /gene="uxuA"
                     /locus_tag="RD521_002152"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDT7771859.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20739..21452
                     /locus_tag="RD521_002153"
     CDS             20739..21452
                     /locus_tag="RD521_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7771860.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21659..21808)
                     /locus_tag="RD521_002154"
     CDS             complement(21659..21808)
                     /locus_tag="RD521_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771861.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22204..22404
                     /locus_tag="RD521_002155"
     CDS             22204..22404
                     /locus_tag="RD521_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7771862.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7739 a         3451 c         4638 g         6612 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcatgcgata
     1081 acgttaggta aagagctact agatcaagta ttagagatgc cctcgattga taaagcagtg
     1141 aggggcttcc aagaagattg ggtgcaaact ttccaagcag aaacgattgg tgaactaaaa
     1201 aaggtcgctg attacttctg aatcaatttc tccataaaat aaacgatagc cttctcgaaa
     1261 actcgaggag gctatctgac gattcaatat tcattaattt aaagtttggg tgactattac
     1321 taactattac ttatttctca tttaaacgat gtattcccta acctctaata catccttgta
     1381 actctgaagt tacgccagtt ttgaataaat ttcataaaaa caaatgaaaa gtgtcgcata
     1441 aaaatatctt tcgtgtttct ctagtataac agaataagaa gaacaagtcc agaagggata
     1501 tttttagtct tttttaaaat aacttttcag tcgagtttga gtttatgata gttgtttttg
     1561 aagcgatacg gcaagtgaaa aaatagggaa gcatgaaaga atctacttaa tggcgcattg
     1621 attcatgtga tagaaataaa aaactttttt aaattatatc ctcatgattg taacaaatcc
     1681 agtgatttgt tttggtacaa ataaaaaaag gccccgatga agttgtggca aaagtggttt
     1741 gcctcaaaaa taagaaggaa tttccaaaaa tcacttctca aattttgcca catccccaat
     1801 tgggtcaaat ttgtttattt gaatatattt tttacataag gatctttaat agttacattg
     1861 tcttttttca tcactttacg gattgtttta tccatgaatt ccgtatcaag agtttttgct
     1921 tcggtagcaa ttttttctag ctcagatttg tatttgtctt tattagaacc tttatcctgt
     1981 ttttttacca tttttactag ataatagctt tgctgataag tcgttggatc tgttacagga
     2041 atgatatcag aaacttcccc gttttttagt ttaaatgctg ctttttttac ttcagtagga
     2101 atctctggat tggttgaatc aaaattcatt tttccatcag tttcctttaa tgttttattt
     2161 tttccataag cttgaacaag cttggagaaa ttttctcctt catttgcttc tttttttgct
     2221 ttatctgctc cttctttagt cgaaaataga atgatctgtg tactaacttc tggatagaat
     2281 tctttccaag cagtgtctag atctttatct gttaatttta cattggcttt caaaccgtat
     2341 tggaaagcca gcatctgttt aaagagtttc ttatatgtct cttctgtttg gccaactgct
     2401 tttaattgtt ccttgtagtt tggaccgagt tttttgattt gttcattata ttcttttgtt
     2461 acttcctttg aagaaatctc ttttccgtat acatcatcaa atgctttgaa gacaatcatt
     2521 ttttttaata gtgtttgatt atttgattta acttttgggt tatcataaaa atcctgaact
     2581 gtaagtgttc cacctttaaa cgttgcaatc gtttcattgg acttgctaca acctgagaat
     2641 agtaaaattg cacatgctga aaggcacata attagtagtt gttttttcaa aaaatttccc
     2701 atcctttcct tattaaaaac agtctaacag aagaattagg gagacagtct ttcattttcg
     2761 taaggttgct gtagatattc ttagttcgta aaaatctatt ctctctatga tggagaacat
     2821 ttacatagtt ttcacgagac aagaaagaaa agggttgtta aacttaatat aaaggaggga
     2881 tgaaagatga tcaggattat tcggttgatt ggatctgttt taatagtctt agttggtttt
     2941 gttttaggaa ttcttgtcaa taatgcactt gcggatatgt ttattggaaa tagtgaatgg
     3001 attggagcaa ttgccggtac ggtcggacat ttagctgttt ttttactagc tttatttctt
     3061 gctagtaagg tcgaaggaaa aagaatagaa gattatggtg tttctgttcg aggaaaagac
     3121 tgggggtatt tagcgggagg tctcgttgtt ggaattggag tgtttctgct tattactagt
     3181 ccattatatt tgacgggggc gtatcggtta gattcaggaa acgcaaacat tgtgccgctg
     3241 ataacgagtt ttattttatt tattgcagtg ggggcatcag aagagctttt attccgtggc
     3301 ttttttcagc atcagatgct tagatttggt ccgttaatag caatgattgg atctgctgca
     3361 ttatttgctc ttttgcacgg attgaatcct aacatgacat ttctggcagt tttcaatatc
     3421 tttttagcag gttgcttttt cagcgcactt atctacagta caggaagttt atttacggct
     3481 attggtgctc atattacttg gaatcggaca caaggagcta ttttgggtat accagtcagc
     3541 ggaacgcaag aacaaggttt tttctccact ctgatccaat cgcaaaatac aataattact
     3601 gggggaaact ttggggctga agcatctata tcaactacat tattcttgtt agtcttgatt
     3661 gttggtttgt tactctatgc atataaaaca aagcggatga agaaatacac tgctgtttat
     3721 caataaaaga tgaagaaagc aaggctgata tcggtcttgc ttttttaagt ctgtcattga
     3781 ttaaacgaaa aagtaaacaa attcaagcga atacacgaca tcttttgtta gattttcaca
     3841 actgactgtc gtgttctcat cattttcatt ttatataatt cctttatcaa atagaaggta
     3901 attatatgga gagtgataga aatgaagaaa acagcgaccg tattatttgt tattcttggg
     3961 ctgagtataa ttgcagcaat gattggaatc agtacttctg caaatgaacg ggcaaatgag
     4021 aatctttcat ttactgtgaa aactgatcgg attgtttatg acatgacaca acaagtcatt
     4081 actattccag tcaaaccaaa caaaagcgtg aatgctagtg atgtacatgc tgttctaaca
     4141 tatggttggg atggtaatgg aagttcagaa aaagttattg gggaagttta tttaaaagac
     4201 gtacaatgga ccgctggtat tgagtataca ataatgattt ctgcagaact atcgattgat
     4261 gagatcaaga gtaaagataa agttgatttg attgtttttt atgatggaca gatgacgatc
     4321 acggagaatt tgaaaccaag cagttggaca gttgttggac cgccaagcag tacggatagc
     4381 acaagtagtg aaagcagtac agaaaacacg agcggtgaaa gcagtacaga gagtacgagc
     4441 agtgaaagta gcacagaaaa cacgagcagc gaaagcagta cggagagtac gagtagtgaa
     4501 agtagcacag gaagcacgag cagcgaaagc agtacggaga gtacgagtag tgaaagtagc
     4561 acaggaagca tgagtagtga gagtagcaca ggaagcacga gcagcgaaag cagcacagga
     4621 aaagtaaata atgaacttga tttggtacct atcataccaa ctaaggaaca aaagagaaat
     4681 gcagggagca acctaaaaag tgtgctaaaa cagcctgatt ttgtttctcg tggaattgat
     4741 catcaagaaa aagcgaaaga aacagataat agaaaaagcg atttaccaga aactggtagt
     4801 agaaccttga acaagctaat tacatggatg ggtgtgttac tcattttgat tgtaggagca
     4861 agctattttc gttctctctt ccatcgagtg aaataaagaa taaaagcaac agatctaaaa
     4921 ttgaataaaa cgatagattt ttgagtgaat atttcattcg agagtgctgt attgcctggc
     4981 gaagttgtag ctttgccaga tctatttttg tttactaaca tatttgagaa acaggaaata
     5041 acaggaacac aaatggaaaa taagagcaag aataattgac aatgcacact gcgcatggta
     5101 gtatctctga aaaatcagta acgaatctat ttgtacgtta ttcatgtagc tgtcggaata
     5161 agtctaaata atagataaaa aggagtatgt actttgtttg aagaatttat tgatatcaat
     5221 gaaaggcaag tatatcaatt tttgaactat tgttatgaaa gagacgagaa attatatgtg
     5281 gtgaaggata tcgctttaga tctaaactac accctggcta agatgaattc ggtgattcaa
     5341 caagcagaat cgttttgcga aaggtatcca gaatataaat tatcttttct ttcagaaaac
     5401 aagatgataa aagtcgagtt ttcttctcag tttttactga gtaaagttta ttctatactt
     5461 ttagagggaa cgattggtta tattttactg gattcacttt acaaaggtac ttatcaatcc
     5521 ttagaaaatc ttagtcaaaa aattatataa gtttgcgtac gattcaaaga aaattaagag
     5581 aacttaatac tattttgaaa aactatcatt tatcttgcag cttgaaaaaa ggtaatccga
     5641 tagaaggaga agaataccgt atccgatact tttttcactt gatgtattgg cagtcattcg
     5701 acgaaaatca ttctgatata ttatcaagta taaaaaataa acgtcaacta ttagaaaaat
     5761 ttgcgaaaca tgcgccatat acgcgtagta tagatagaaa gaaatggatc agcctactaa
     5821 gaatttcgtt gcaacgaatg aaacaaggcc actcgattac gtatgtccca gaagagatga
     5881 acaaatttgt gcatccattg ataacaaaag aagagtttac gcattacata ctagagcctt
     5941 tttttgaagc aaacttctgg tcgatgaatc acatcactcc aacagagcaa gcttaccttt
     6001 attttatgtt tggtgtaatg aacacctatt tggaagaaga tttgttggat actccagata
     6061 aagtacaaga aagttggggc tggattgaat cgtctattgt agaaaatttt gaaaaaaact
     6121 tcaagatttg ttttacaact tcagaaaaaa attatctata tgtcaatttg gcaatgatcc
     6181 atttgtattc aagggttttt gggacaaaaa agaaggtgga tgcttttgga aaatcagcag
     6241 aagacagaga ttttcagcat atatttccag tgatgtatcc aatcatgcaa cagtttttta
     6301 gtggggtagc tcgcagaatt ccagagctta atacttatta tgaaaagaac agacggttga
     6361 tttttcaata ctgtatgtta gtcagggata tttttataaa acacgaacaa cctgtcatat
     6421 tctatattca atcaaagtat ggtaaaacac aggaattatg ggcaaagaaa aggatattat
     6481 ctatgactga acgcccaata aaatatagtg catccgctga tcagttacct gatattgtca
     6541 ttagcgatta tcctatcgac aaaaaggcat atgatcaaac gaatacacag atattttact
     6601 ggaatggaca acctacaaaa aatgattggg cgcgtctgaa agctgaaatc ataaaaatca
     6661 ggaatgagaa aaatagatcc atattggaaa aattggattt cgttaattaa gaaaagcaga
     6721 ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat cgacaagatg
     6781 accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa ggaacatcct
     6841 gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga taatcggttt
     6901 tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac tgagttcatc
     6961 tattctgtga acaagatttt tataactaat agaatgagta gagagcattt cggtcacgcc
     7021 gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt gacgaattaa
     7081 gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc gtcttgaaga
     7141 cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata gctaagtatt
     7201 tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt aaaataagaa
     7261 aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc aagaaaaaag
     7321 aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga tttacaaaaa
     7381 ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc attagtgaat
     7441 aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca acacgaagat
     7501 gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc tattcctgag
     7561 cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa tttgagtgtt
     7621 ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc agagtattgt
     7681 atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat gtgtagagga
     7741 gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc tttttacgag
     7801 aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc ttcgtttaaa
     7861 aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg agaaaataat
     7921 cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt ttgcgtttac
     7981 atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa caagtattaa
     8041 agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg acagtatatg
     8101 gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa actactgatg
     8161 ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa acaacggttg
     8221 agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac caacaaacag
     8281 aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa gtaagtgaca
     8341 aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat ggtaaagaat
     8401 atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca gaagacatca
     8461 agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat ggtgtagata
     8521 aagagattat caaacatctg attgctactt atctattgta cgatatcgct tttgcaaaaa
     8581 acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct acagaaaaca
     8641 caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa caagatggca
     8701 aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca gtcatgatcc
     8761 atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac aatgattttt
     8821 ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat agcggaggcg
     8881 attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata aatcaagaag
     8941 ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga gcagctacga
     9001 tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt gaaaattgcg
     9061 gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta gcccccgcac
     9121 taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat gaagatttat
     9181 ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat acaaaagctg
     9241 ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat ggaacagcag
     9301 atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga gggtataaag
     9361 atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac catgctgcct
     9421 acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata aacgtaaagt
     9481 atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag ctttacggag
     9541 cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa ttagcaaatg
     9601 ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt ttctattaca
     9661 aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca gacaataagg
     9721 gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt cagatgacag
     9781 tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact ggagaaaaga
     9841 aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt atcacatgga
     9901 tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt gattgcagta
     9961 ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat cacaccaaag
    10021 ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca agtatatgcc
    10081 cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga aatatcctat
    10141 ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga aaagaaaaaa
    10201 ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa aaatgattgt
    10261 cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa tatagattat
    10321 gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga agccattcaa
    10381 gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat tggtggaaat
    10441 tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga agaaattaaa
    10501 aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt tatttctttg
    10561 tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc tagctttctt
    10621 tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc agagaaggtc
    10681 aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt cttaacggat
    10741 ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt tgagaaagcg
    10801 ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa ccatgaatat
    10861 cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt aatgtttttc
    10921 aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct ccaagtcaca
    10981 aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact tgtgactgag
    11041 gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac acgattattg
    11101 tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc gtttaaaaaa
    11161 agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg gtaagcaaaa
    11221 gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac aaagatgaat
    11281 agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag ctttcataag
    11341 cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa atgtgacaag
    11401 tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat ggctaatgat
    11461 aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac gtctagacca
    11521 ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa agaatttccc
    11581 caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc aactgtgaaa
    11641 taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa ggaaatactt
    11701 aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa taaaaaaaag
    11761 aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac aagtaactcg
    11821 aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac ccaccgacta
    11881 ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat ttggtagtgg
    11941 ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt tgatttcaga
    12001 aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt aaaacatcaa
    12061 aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta aagcttcaga
    12121 tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt tagcaaggga
    12181 agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta cggtttcagc
    12241 aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac agtctttttc
    12301 cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag aagttgcgat
    12361 aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac cgccaacacc
    12421 gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta gatccttttg
    12481 ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca ccttttgcgc
    12541 catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag cagatagatc
    12601 attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac caagtttttt
    12661 tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat tgctaatagg
    12721 actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct gttcttttgg
    12781 gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca tgagaattcc
    12841 tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag atacaaggag
    12901 agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa aaattttttt
    12961 gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa ttggtttggg
    13021 aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag tacaaggact
    13081 cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg cttatcaaga
    13141 gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa tgcttcctgc
    13201 tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac caggagacat
    13261 tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag aagagtttca
    13321 gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag gagcacgaca
    13381 tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg aacaattatt
    13441 caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa gcggacactt
    13501 cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg gtgaaggatt
    13561 ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac gtgtttggaa
    13621 tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt taaacgaaca
    13681 tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa aacggacggt
    13741 tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt tgttcatgag
    13801 gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac taagaaatgg
    13861 gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat catatagcaa
    13921 tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga ttaaagtcag
    13981 ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa ctagcggagt
    14041 tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga cgagattttt
    14101 ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac aacaatattg
    14161 atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct ttagttgaac
    14221 gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga cgcacgacac
    14281 aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc gtcggtagga
    14341 cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc ggagtaatcg
    14401 aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac aaatcagatt
    14461 tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg gaacaatcag
    14521 atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt tctgataaag
    14581 aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc gctctagttg
    14641 atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat gccacagatg
    14701 ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa caagtagtga
    14761 ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt gaaaaatgtg
    14821 tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc cagacaaaga
    14881 gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga aaacaggccc
    14941 tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct tgaaatcata
    15001 cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg cgctacctaa
    15061 attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt ctggagaatg
    15121 tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat cgatttcgta
    15181 gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa tcttgcaaga
    15241 atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac acctcttgct
    15301 gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta cagacaagct
    15361 gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata tttcattgcc
    15421 gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga acaagatttt
    15481 ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg caaagaagag
    15541 attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact gacatcggag
    15601 ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg attaggagat
    15661 aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa atatattccg
    15721 gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta tcaactggca
    15781 ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga attagaactg
    15841 ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat agataaaatg
    15901 gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt aacacagaaa
    15961 tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg aaggaggagg
    16021 acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg tagccgcggt
    16081 tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc ctttgatgac
    16141 tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg ttacagccgc
    16201 tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag ctgaaccatc
    16261 tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc cagaagcagc
    16321 gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt ttcgtttttt
    16381 catcaatact tttttaggta aatatacaga caaagctgta gaagatttga atacaaaagc
    16441 tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat tcgttccgct
    16501 tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca aagctttaga
    16561 gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg caatcggtat
    16621 cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct tccttgctta
    16681 ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg ccatttttgg
    16741 gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac agcctgccac
    16801 aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg gattttaatt
    16861 taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc
    16921 acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac
    16981 tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa
    17041 ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac
    17101 actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcaa
    17161 agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac
    17221 acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc
    17281 gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc
    17341 gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca
    17401 agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac tggtgttcag
    17461 acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac
    17521 tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag
    17581 gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac
    17641 ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata
    17701 ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt
    17761 gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta
    17821 tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa
    17881 tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt
    17941 acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt
    18001 ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa
    18061 gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa
    18121 tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc
    18181 gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta
    18241 ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa
    18301 atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag
    18361 cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt
    18421 atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt
    18481 tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt
    18541 catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac
    18601 tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat
    18661 aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta
    18721 gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa
    18781 acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat
    18841 gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac
    18901 agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca
    18961 gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa
    19021 gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg
    19081 cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc
    19141 aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta
    19201 atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg
    19261 gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac
    19321 atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc
    19381 atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc
    19441 aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca
    19501 atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa
    19561 caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa
    19621 aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg
    19681 gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa
    19741 tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat
    19801 tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt
    19861 agcgatccac gatgcaatca aggcagggac agatgaacga gattactata ttgataacta
    19921 tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa
    19981 accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc
    20041 tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat
    20101 acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt
    20161 cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac
    20221 ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca
    20281 tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga
    20341 cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg
    20401 atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg
    20461 catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac
    20521 ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca
    20581 agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc
    20641 tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata
    20701 tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca
    20761 tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa
    20821 aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc
    20881 aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata
    20941 tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag
    21001 tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata
    21061 caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc
    21121 tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac
    21181 cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat
    21241 ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca
    21301 tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg
    21361 taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac
    21421 gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt
    21481 atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag
    21541 tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct
    21601 accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt
    21661 atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc gctacatgat
    21721 tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag gttactcgat
    21781 attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat acttgctgaa
    21841 aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa ttgaaaggtt
    21901 ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa acaagaaaac
    21961 aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca atagaagaca
    22021 atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg atatattaaa
    22081 tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa aactcctttt
    22141 gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag gaggaattta
    22201 tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg ctttatcact
    22261 ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga cggcttcaaa
    22321 actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg tggccctcaa
    22381 gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt cgaaagaaaa
//
LOCUS       DAPBCS010000049        22024 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPBCS010000049 DAPBCS010000000
VERSION     DAPBCS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.050"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(59..1369)
                     /locus_tag="RD521_002156"
     CDS             complement(59..1369)
                     /locus_tag="RD521_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDT7771863.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1381..2799)
                     /locus_tag="RD521_002157"
     CDS             complement(1381..2799)
                     /locus_tag="RD521_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7771864.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3039..4607)
                     /locus_tag="RD521_002158"
     CDS             complement(3039..4607)
                     /locus_tag="RD521_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDT7771865.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4611..4892)
                     /locus_tag="RD521_002159"
     CDS             complement(4611..4892)
                     /locus_tag="RD521_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDT7771866.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4889..5812)
                     /locus_tag="RD521_002160"
     CDS             complement(4889..5812)
                     /locus_tag="RD521_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDT7771867.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5809..6687)
                     /locus_tag="RD521_002161"
     CDS             complement(5809..6687)
                     /locus_tag="RD521_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDT7771868.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6674..8011)
                     /locus_tag="RD521_002162"
     CDS             complement(6674..8011)
                     /locus_tag="RD521_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDT7771869.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8014..9117)
                     /locus_tag="RD521_002163"
     CDS             complement(8014..9117)
                     /locus_tag="RD521_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDT7771870.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9191..10699)
                     /locus_tag="RD521_002164"
     CDS             complement(9191..10699)
                     /locus_tag="RD521_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7771871.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10665..11357)
                     /locus_tag="RD521_002165"
     CDS             complement(10665..11357)
                     /locus_tag="RD521_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDT7771872.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11379..12353)
                     /locus_tag="RD521_002166"
     CDS             complement(11379..12353)
                     /locus_tag="RD521_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7771873.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12653..13525
                     /locus_tag="RD521_002167"
     CDS             12653..13525
                     /locus_tag="RD521_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7771874.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14107..16305)
                     /locus_tag="RD521_002168"
     CDS             complement(14107..16305)
                     /locus_tag="RD521_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7771875.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16537..17529
                     /locus_tag="RD521_002169"
     CDS             16537..17529
                     /locus_tag="RD521_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7771876.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17589..19010
                     /locus_tag="RD521_002170"
     CDS             17589..19010
                     /locus_tag="RD521_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDT7771877.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19003..20502
                     /locus_tag="RD521_002171"
     CDS             19003..20502
                     /locus_tag="RD521_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDT7771878.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20541..21935
                     /gene="uxaC"
                     /locus_tag="RD521_002172"
     CDS             20541..21935
                     /gene="uxaC"
                     /locus_tag="RD521_002172"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDT7771879.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6668 a         4494 c         3823 g         7039 t
ORIGIN      
        1 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
       61 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      121 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      181 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      241 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      301 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
      361 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
      421 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
      481 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaattga
      541 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
      601 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
      661 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
      721 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
      781 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
      841 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
      901 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
      961 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1021 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1081 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1141 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1201 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1261 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1321 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     1381 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     1441 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     1501 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     1561 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     1621 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     1681 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     1741 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     1801 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     1861 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     1921 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     1981 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2041 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2101 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2161 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2221 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2281 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     2341 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     2401 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     2461 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     2521 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     2581 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     2641 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     2701 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     2761 ttttactgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     2821 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     2881 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     2941 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3001 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3061 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3121 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3181 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3241 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3301 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     3361 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     3421 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     3481 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     3541 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     3601 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     3661 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     3721 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     3781 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     3841 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     3901 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     3961 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4021 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4081 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4141 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4201 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4261 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4321 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     4381 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     4441 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     4501 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     4561 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     4621 tttctcattc ttactcgata taaactgaat gccccagctg ctaaaaaagc aagtgaaata
     4681 aacaacacaa cggcaaacag accagtcatg tcctgtaata atccaaatgt attttctaaa
     4741 gacaaagttg aatgatctac agcaagcgac tgataacttt ttttaaactc tgacgcagac
     4801 atattactta aagtaactgc taacccgcca aagaaaatca aagatagact gccaccgacc
     4861 attcctagca atagttccca ttttcttttc atttctgaac cccctgcttt tctaaatgta
     4921 tccattcagg tcttttgatc gatgtttcat aattgttatt acgttcagag aatttgacag
     4981 tctgttcatt tttcttgtca tcccaattat cccaaccagt tgggtgaaca tgcccaccta
     5041 tcttacacgc ttcaaataag acatttgcat acggacgcca cggtcttcct aaatagacgt
     5101 ttctagtatc aggttctgca gtcaaggtac agctttcaaa acgataccct gttttttgat
     5161 ttttcggcgt agatgctgct gtgatgtatc caggattttt actggctaag ctatggatat
     5221 gacactgatg aaaaacggca tctgccccac caaatatgaa atccaccgtt ccttcaatcc
     5281 agcaatattc ataggtctga cgacaatact caggattggc agaagccggt gtacaaaaag
     5341 gagtaccatc tttttgcaaa tcaggcaacg gacccgtgca taaggtatcc tgataaccag
     5401 atagtcgaca gttttttaaa gatacgcgat ctcctgaatt gaataaagca acagcctgtc
     5461 ctaccttttc tcctggacct gctgtatttt cgatgtgaag attttctaat acgacattcg
     5521 atccttctaa gaaaaaagta gcacttcgga aagtccctaa ctggctaccg tcaagatact
     5581 cctgttttgc aaaacgatta ccggagataa ctactgttcc tgctcccaca agtttaaaat
     5641 tggataatct gctttctata tattcatcat atcttccttc ttttactatc aagacctttg
     5701 gatggctatc cggcaggcct gccaaataat cgattccttc ttggatagtt tcaaaatccg
     5761 tcggggtatc tatagcattc tttccaatta tcagtttgtc tttcatcctc ataaatgctc
     5821 ccttatgaat tgtttccaaa tatttctcat ttcttgtgtt tccatatgac cgcctgttaa
     5881 agaaaaagaa gaaaagtttt ctggactacc cattgattga tactttttgt ccagcccttt
     5941 tctcagcttc agtacgcctt cgtccaagca catcgtatcg ttttttccaa ctaagctaag
     6001 acgagggcgc ggagcgattt tttcttggat tgctaacgtt gtataagctg tcaatagatt
     6061 tggcacatag taataaaacc catgatgatc taggcggcga tgtagcaaaa gtgtttcgat
     6121 gtccacctgt gccgctagat caatacaaac tttgacacga gaatccaatg ctgtcaccca
     6181 ccagcttaat aagcctccca tagaaagacc aatcgttgcg agccgttgag tatctgtgtc
     6241 ttcccttgtc tccaaataat cgatcaagct gatcacatca taaatacgca ttccccataa
     6301 cgtttttcct gttaataaaa attgtttgaa caactcgctt tcagaaatac ctccgcgttc
     6361 ttcaaatccc caagcatcaa tcgaccaaac agcatatccc attcctgtca gtacttctgc
     6421 aaaagatgga gaagctaagt aaggagcacc atggatcaat tcacttttcc cgactgaaaa
     6481 atctccgccg tgagaatgca ggtaaaggac cgtcggaaac ggtccttctt tttctggata
     6541 agcatagagg gctggtactg tttccaattc atttaagtac aattgatagt attcaagcat
     6601 aaaaccgttt tgtttttctc ggtataatcg ttttccacta ggggtatgtc tgctagatgg
     6661 agcacctaaa aaatcattca actgcataag caaagttttt acgaacaacc agttcatcca
     6721 tattttctga ttgatttgat tggaatttct caatactgat ttctttagta ttttctaata
     6781 gaaatgctgg tccctcgtga ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa
     6841 agccaatata aaatccttgt ctgctcattt cctctattcc tgtcatcata gcaggaacac
     6901 cgggaactgc atcttgggcc atcgagacat cgatattgtc aaatgtgatt tcttgtatga
     6961 attgttccgc taatccataa ataaaacctg cagaagcatg gacattcctt gccgtgatat
     7021 tcgaaaaatg gatccttctg aatgctgatg tccgttcatc tatcggataa gccttttttt
     7081 cccatacata cggttctttc cctcgcggac cacagaaata ataaagattc agtataaatg
     7141 gacacatcac attatccatg actatattgg atactcgaat atcttcaacg atcccgcctc
     7201 gacctcgtct agatttcaat cgtattcctc gatcagtttc ttggaagata caatttgaaa
     7261 tggtaatatt acgaatacta ccactcattt cacttcctaa tacgacaccg ccatgtccat
     7321 ggaccatcgt gcaatttgtg atcgtgatat tttcgcaagc gatcctttcg tacgtatctt
     7381 cagtccctgc cttgattgca atacagtcat ctcccacgtc aatatggcaa ttactaatac
     7441 gcacattttt gcatgattct ggatcaattc cgtctgtatt tggtgaatcc gctgggttca
     7501 agatcgtcaa gttatcaaac gttgcattac tgcaaagaat cgggttgatc gtccaacttg
     7561 gtgactggat caatttaata tctttcacag taattcgatg acagttatgg aaactcatca
     7621 atttaggacg aggataagcg agattatctg gttcatttct aaaagtatgc caccattttt
     7681 ttccatttcc atctaatgta ccgaaaccag tgacagagat gttctctaca ttttgagcat
     7741 agatacagga agcataaact tttcgatgga ctccttccca acgtgaatga acaacaggat
     7801 aatcttttgg atcgtcagag aatttcagaa ccgctcctgc gcttaaatga agttcgacat
     7861 tgctttttaa aaataaagct cctgttagaa attcacctgc tggaacaact acggtcccgc
     7921 ctccgtcact tgctgcagca tcaatggctt gttgtattgc ttcagtattc aattcatcaa
     7981 ttgatgcgcc aaatgtcaaa atatcataca tatttataat gcctccactt caatcaatgc
     8041 ttgcaagaat gcaccaacgc ctttttgatc attacaaata attggttcac tgatgtagta
     8101 agcatatgaa ccatctcgct gatctgctcc gcctaaccct gctacttgac agtttttatt
     8161 caagttcagc cagccttctt ttgtttccag aacaaattca tcaagtaatc cttgatgtga
     8221 tttcttcact tgtggaagcc attcttcttt gttcagcagt cccaaacgta ttcctttagc
     8281 cattgcgtaa acaaacatac tactgccgga agcttccaga taatttcctt tccgttttgt
     8341 ttctgttgtt acttgatacc atgtcccgct gttttcttct tgatatggaa gtaatgcagc
     8401 cagtgtcttt tctagaattt ctttcagcaa agaacaatct gtttcttcat gaagtaattc
     8461 ataggtatct gctgcagcca tcaaatacca gccaatcgat cggctccaaa aatgatggga
     8521 tagacctgtt tttggatcag cccatggttg gaccttcttt tcatcccatg catgaatcag
     8581 caaatttgtt tttggatcga atagatgttt ataactgatt tcaaactgtc tcaaaacatc
     8641 ttggtaatct ttttcttcac taaaattctg gataaattca gcataaaaag gagaacccat
     8701 atataagcca tccaaccaaa tttgataagg ataaatttct ttatgccaaa aagctccttc
     8761 ggatgtgcgt ggatggtttg ccaactgctg ggaaaggagt tctgctgctt ttttatattt
     8821 ttcttcgcct gtttctttgt acaataaaaa gaaaagcttt cctgtattta catggtcgat
     8881 attgtattct tcaacgctgt agccacggat cgttccatct tcttgcacga aatagtcgat
     8941 attgtctttt atgaaatcaa aataaatttt ttcttgggtt cgttcccaaa gtaattggaa
     9001 ccctttaagt acgacgccat agtcataaga ccattttcct ttataccact tttcttcata
     9061 taagcgaggt gttctttcca tgatactttt tgctaatttc tttgaccagt tttccattat
     9121 ttgctctcct ttaaaaatta aaataggatt tgggacaaat accaagaagt atttgtccca
     9181 atcgtctata ttactgtgcc gctgctttgt atgcttcctc gtactcttgt gtgattcgat
     9241 cgcctccttg tgctttccag tcttctacag cttttttgaa tccatcttcg tcaagctgtc
     9301 ccatgatgta ttgcaccgta gcatcaatga cgattttttc aatttctgat cctacttcat
     9361 tgttcgtcgg tgaatccaat ggcacagaag gatccattac agcaaattct tcattttctt
     9421 tgatcaattg attagagaat tctttttcag ggtctgtatc tttgaaagag aacgtcactt
     9481 cactaggacg agagcttgat aacggttgca cctcttgttg ccaaagatcc atatcgataa
     9541 attcgataga accatcatca ttcaattcat aatgcgttcc ttcgataccg ccagtcatca
     9601 atttatacac atcttcttct aaaagatcat caacaaattg aagtaatttc ttcaattgtg
     9661 cttcgtcttt tacttgagat ttgggaaatg ctagcaagcc gccaatacca tttccttctg
     9721 accaaacatg ataaccgccg ccttcaccat ctgagatctt gtttacaggc actaattcca
     9781 tatcatcttg aataccttga cccatcgtct tcagattctt gatatccacc attccagtgt
     9841 agattccagt tcttccttgt gcaaattgtt cttgctgatc agtcttttgg gtaactgcaa
     9901 agtcttgtgc caaatagcca ttctcaaata gatctcttga aaaattcaaa gctttcatat
     9961 actcttctgt atcaaattct ggagtgaatt tgccattatc gtctacagcc cagccatttg
    10021 gtgctccaaa ataagaagtg aaaagtttaa agctggtata acgaatatca ctaggcgcac
    10081 gatcgccaaa accaactgtg tcgtccacac catttccatc aggatcgttt tctgtaaatt
    10141 gtttagctac ttcataaagc tcatccatgg tagttggaac atctagtcct agattatcca
    10201 accagtcttt tcgaatgact aagccagcac gagcgtagtc tttttgtatc ggtacgccgt
    10261 acaaggcacc gtcgatactc gcagcttcga ttctatcttc agaaatttga cttaaatttt
    10321 tatagtcttt taaatattct gatacatccc agaatacgcc tgattttaac gcattgcgga
    10381 cagaagagtt tttcaacatc gcaatttgaa gtgtaacgat atctcctagt tcatcagaag
    10441 ccaatgcagt tgttaatctt tcttcttttg aagcatcagg aatccagttg aatgtcagtt
    10501 ccgtatcggt tttttcttcg atcaagttca caatcgtgtc tgtaggcggt gaagcatgat
    10561 gcaaaatatt taaccaagtg attttagctt ttccatcttc tgtctctgaa gaactgttac
    10621 ttgaactgct gccgcatgcg gcaagtgcta gaattcctgc aaaactaaga cctaatttta
    10681 cgagcgttgc ttttttcata aattttctcc ttcactgttt taataatatt tttgttttct
    10741 tctgtttttt ctgtatatga gttcttccat tccaaatggc aaaatataga atttgctgtc
    10801 cacgtgactg cggaaaagaa gagactgcca ccgataaagg ttaaaacacc tggcattgtt
    10861 tggatgatca tcagataaat ccctaccata cttaatatca acactaatga gtaatgaaga
    10921 tgtgagaatc cgaataaaaa tgccattttc attttgaaaa aatatccttt ccaatcataa
    10981 tgagccatga ttgggaaaat atagagtgct gagagcacat atatgaacgc agcaatatac
    11041 aaagcgatcc taagatacca attgtgtatg aaagaaagat ccacaagtaa aacaatcccg
    11101 atggcgccat atatccagct catcagcaag gtttcacgat agttttcttt aaaatatgtg
    11161 aagtacgttt tcgtcaaagg aaacgtttct tttgagcgca gccattttct aatcactgca
    11221 accatcgcat acgatgcagg accgaaagta aaaactccca aacctgcgat gactaaaccg
    11281 ccccacagaa aattcaaata aaccaactgt aaaatacgat ttagccttcc gtttatttta
    11341 tctaatgact gcagcatatc tttccctctc tttctatctt agtaaacgcc gtcttctcct
    11401 agctttttgg caatcttatt agctaaaatt accataaaca atcctacgac ccctttaaac
    11461 aatcccattg ctgtactgaa acttaactgt ccattcttca atccagctgt ataaatatag
    11521 gtgtcgaaaa tttctgcaac gcttcgattc aatgaattca ataacaagta catatgctcg
    11581 aaccctaagt ctaatgtctg accgattttt agaatcagca aggtaatgat tactggacga
    11641 attgccggca atgtaacatg ccatgttttg cgtaagcgtc ctgctccatc catttctgca
    11701 gcttcataca actgtgtatc gactgcagtt atcgctgaaa ggtagatgat cgtagaccag
    11761 cccaactctt tccagatcac ttgtccgata tatactgtgc gtgtccattc aggggatgtt
    11821 agaaaactga ttttttcaaa tcccattgtt gcaaggaatt cattcaatac ccctccgtca
    11881 acattcaaaa atacataagt aatcgaaaca atgattaccc aagacatgaa gtgagggatg
    11941 taaatgatcg tttgaatccc gtttttcaat cgtttgttcg ttacttcatt taatagtaaa
    12001 gacaaaatga ttggcatcgg aaaaaacacg aagatattta atccgaacaa gatcaacgta
    12061 tttcttaata atgtaaagaa tgtcggttct gtaaacaaac gaatgaaatg tttgaaaccg
    12121 acccaaggac ttcccattat tcctaaaaat ggttgataat cttgaaaagc aataacgagt
    12181 ccccccatag gaagatacct aaaaatcaag aaataaagga ctcccggcag aatcatcaca
    12241 tataaaaatt tattcgtttt gattcctgca attatttttt ttcgtcttct gagcttttct
    12301 tcttttttcg atagcgtcag ttgaaatggt tcttgtttct ttacactttc cataagcccc
    12361 tccttagttt tgtgaatgtt tgctgcttat gacttcatga tagataacta tctgaattac
    12421 gtatataatc atttttatcg taagtaaaaa cgcttacata gcaatgattt ttgtgctata
    12481 caaaacgata aagtgtattt tttcacaaat aaactacgca tagcaaacct tctatttttt
    12541 tctatctgac acttagcata ggaaatgatt atatacgtaa tctttcaccg atgataaatt
    12601 gaacatatag aaacagaaac atttaattta ttttgaggag gagcctatct atatgaaaaa
    12661 gaaagtctct ataggagaaa gaagttttac acttttcaac aatacgtttc taattttgct
    12721 agctttgatc tgtatcgttc cttttttaaa tatcatcgct acatcttttg cttctacaca
    12781 agaagtcgtc gcgaaaaagt ttatcctttt tcctactacc ttttctttag acgcgtatcg
    12841 ctacattctt tctacgccga ccatttttag agcacttgct gtttcgattg gtgttacagg
    12901 tgtcggaacg atcgtcagta tgtgtgcaac ttcactgatg gcctacggtc tttccagaaa
    12961 gtatttgttt ggtcgaggtt tcgtcaactt tcttgttgta ttttctatgt tgttcagcgg
    13021 ggggatgatt ccaacgttct tagttgttcg ttctctaggg ctggtcaatt cttactgggc
    13081 catgatcctg cctgtggctg ttaatgcaat gaatatgatc attatgagaa actttttcca
    13141 agccttgccg gatagtttgg aagaatcagc aaaaatggat ggttgcacag attttggtgt
    13201 atttttcaaa attatgctgc cattagcttt accgtctatt gctactatct cgctttttta
    13261 tgctgttact tactggaaca cttatatgac tgctattttg tatatcaacg attcatctaa
    13321 atggccgata cagattcttt tacgccaaat cgtcattgta tcaagtggga tgcaagcaga
    13381 aagtagtgcc gttgatgtga ttccaccagc acaaacagtt aaaatggctg ttatcgttat
    13441 tgcaacagtg cctatgctga ttgtttatcc ttttgttcag aaatattttg tcaaaggagc
    13501 actcgtaggt tctgtcaaag ggtgattatg tactgaacgc aaaatggtag ttgtgtcaga
    13561 agtggtcagc ttcaagaaat aagaaggcat ttacgaaaat tgcttcttaa atttttgtga
    13621 aatttcggct tatttttcga agaatctact cctggaacac cgtgtatcag aggttgtgaa
    13681 agagacgttc agccttgagc gatgataaaa actctcacaa acattgcgac gagtaaccca
    13741 caggagcaat aagaagcgat tttgaaaaat agcttcccat atttcacaaa attatgcctt
    13801 attgtcgata gcctgtctct tgaacgccat ttattcgtaa aaaagaactg tgacaagact
    13861 tttgtcacag ttctttttgc gtgcttagcc ttgaaattta actatccata aatacaaata
    13921 ctagggttat tctaacttct tttattgctt gaatcagtcc acaagaaaaa cagcctcttg
    13981 attaaacggc gtttatcttg ttgattctgc ggcaataaaa tcagccagca gcaaaacatg
    14041 aagtgctgtt ttttgctgtc ctcttttatt gcttgaatca gatcacaaga aaaacagcct
    14101 cttgattcac aaatattttt ttcgatattc tcccggcgta atcgattcct tctttttaaa
    14161 gaaacgaata aaattttgcg gatttcgata ttgcaatctt tctgcaatct cttttacgga
    14221 gaggttagtc tctttcaacc atttttttgc tacctctaaa cgatgatttt gaacaaaatc
    14281 accaaatttt tctcctgttt cctttttaaa tacattactt aaatagttag ggttataatg
    14341 caacctatca gcgatcacat ctaaagacag atcacgatca tattctgtgt agacgatatg
    14401 cataatattc tccgacaaat ttttaaattc atgatcagtg attgcttgag agcattctgt
    14461 gattggcaga actaattgtt ctaaaacaag tttttctagt tctttgggat gataattgtt
    14521 cagtgctttt acataaattt gtttcatgtt atcaaagttt tttgaatcta ctcccaataa
    14581 ctgtcctaat tgaacgagtt cattgatcaa gcggaccgaa actacttccc gactcaaagg
    14641 atttttattt tgtgcaaaca atgcatcaat caattcatgt actaaattct tcacttcctg
    14701 atttccagaa cgaatggctt caaacaatcg attttgttct tctatcggat atctaataag
    14761 tgttttttca tgagcagcag gaacgatttc ctgataaaaa ataattgaac ttggaccagt
    14821 atttacacga taatacaggg cttcttttgc ccgatcaacc gattgcttgc tttcattcag
    14881 cgtttcgaac cggttactga tgcctatact gatagttaaa ttcaagtatt cttttacacg
    14941 tttttgaatg agtcggcaat actccatgac ttttttgctt gcttctgcat catctttatc
    15001 cattcgataa atagtggctt gcatttcttc attcaaaacg atcggcagca ttcgattttc
    15061 ctttggcaca atttccgtga tcatgttgtt gatcgctaaa agtagcaaat cacgctcccc
    15121 accgtgacta tcttctaaca gatcgatttg aaccaaagct gtataatagc agtcattttg
    15181 agaactataa ccaaattgct gcaaccgctg atctagttct cgcctttcta ctcgatttct
    15241 aaataaacta agtacaaata gcgtttccag ctgtggcttt tgggtaacta agtgtgcact
    15301 taacgcttct ttttctccta tgatccgact gactgattga gcgaccaaat ctagttcttt
    15361 cccactgaat ccttttgatt taagattcag acgttcctgt atttgagaaa tcggacgggc
    15421 aaatctgtcc gaaaataagt aggaaaaaag accaatgagt cctaccagtg ttaaagacac
    15481 tgcaactagc ccgattctca gattgcggat cattgcgcca attgcactag agtcaacttc
    15541 taatctgtaa atccaattat tataatctga tttcagtact atatacgttt tatctttttc
    15601 tcttatcact ttcagctgat tgtaatctag atccacgtcc gttaatacag ttggttgctc
    15661 tttatttttt actgtctgag tagtaaaaag gttgccaact tcgttttcaa tagaaagtat
    15721 gtcttctcca ctttcaccaa caacttgttc tattgtatgt ttctctatcg tagcaattcc
    15781 caatgcatat ttttcttttt caaacattgg caaagaaatc accatttcga ttcctttgtt
    15841 tttcttttgc caaaataaat tatttttgtt atttatgtac ttctgttgat acttttcaac
    15901 ttcctcttcg gtcagttgac tcaacgatcc attgattact ccccatttac cagccaaact
    15961 gactaaatca taattattat catccataac aatcgtttct ataaaattta gttctttcct
    16021 gatttcagaa taagtggagt aatctgtata tgtcagcgga tgcttgaata tgtccgagaa
    16081 atttttggtc gaaccatacg tagaaaacga atattcaatc gttctaattt tccattcaac
    16141 gttagtttta acttgcctga gcaaattctt tttgtctgtt aaataactcg attcgatcga
    16201 ttcataggta ttctggtaaa tgaataaact aaatcctatg acaagtacgg ctcctaataa
    16261 aaacatcggt aaaaatattt tataaaatac tgtacttttc ttcattttct tcctctcctc
    16321 cttttaaaat aaaaggcttt catttttctg taaaattgat actcatctaa ttaaatgagc
    16381 attccccgct ttcaagaata gcataattaa aaataaaaat ccaagtaatt tttaacgtgt
    16441 gcaatctttt catatcaaag atctattgac ttatcgtagt tatcgtaata tgatgaatgc
    16501 gttaacaaaa agtgaaaaag taaggaaaat gatggaatgg taacgatcaa agatatcgct
    16561 aaagcagctg gcgtttctca tacgacagtt tctcgtgctt tgaataacag cagtttaata
    16621 aaaaatgaaa caaaacaaaa aatacgacaa ctagctaagg aaatgaatta ttcgccaaac
    16681 tatagtgcaa aaggtttagt gaatcaaaaa aaatatctaa tcggactatt tttctcaagt
    16741 ttgcataaag gaacttcatc aaatttccta gcagattcca ttgcaggcat ccattcagta
    16801 ttagacacaa attacagttt atctgttgaa agtatcgatg ttgttgattt aacagaaatc
    16861 aacctgcaac gctacgatgg actgatcgtt atgagtcaaa gtgatgatga tcagcctttc
    16921 atcgactatc tcaaagaaaa agaatttcct ttcgttgttg tcaatcgtca catcaatgat
    16981 ccagcggtta tcaacgtcac tgccgatgat gcggccggtg ttatgcaggc agtagattac
    17041 ggtattggat gcggacatac gaagatcgct tatattggcg gaaagaaaaa ttttcgttcg
    17101 acaaaagaac gggaaaaagg ggtattgaaa agtctaaaaa aagcgagcat ccctgtcaat
    17161 ctagcctatt ttttaacagg agattacagc ttagaaagcg gcctccaaaa catggaatac
    17221 ctattatcac tttctgacct gcctactctg gttttttgcg gcaacgacga catagcgatt
    17281 ggtgcattga gagctgtaca tttagctggc ctccgtgttc cccagaacat ttctctgatc
    17341 gggttcgatg attctccagt cgtctcctat ctcaatccgc cgcttaccac cgtacgtaag
    17401 cctttgaaag aaatgtgtca agaaggaaca aagcttttac tagatttgat cgaaggcagg
    17461 acgatcgaac aatctaaaat tgagctctcc accttgttga tccttcgtga gtcgatcaat
    17521 tacttgtaaa ctacaagtaa ttttatataa aaatgttaac gtgtgcataa cacagaaagg
    17581 aagtttatat gaaaaaactg acaaaagaat tagtgaaaaa aaacgaagca cctgtaaaag
    17641 ttcttcagtt tggtgaaggc aattttatgc gtggatttat tgattggcag attcagcagc
    17701 tgaataacca acaattgttt aaaggtaatg ttgcaatcat tcaaccttta gaacaaggtc
    17761 ttggaaatat gatgaaccag caagataatt tatatacggt cattttacaa ggactcagca
    17821 atggcgaaat cgttgatact tccgagatta tcaccgtagt agaacaaatg atcaatcctt
    17881 atgatgaatg ggatgcttat ctatctctag cagaaaatga cgacttggcc tatatcgtct
    17941 ccaatacaac agaggcagga atccaattcg tggagactga taatttaaca gatactcccc
    18001 cacagagctt cccgggcaaa ctgacagcct ttttataccg acgtttcaaa ttaaacaaag
    18061 ctggctttac catcattcct tgcgaactga ttgacagaaa cggcgaacgt ctaaaagaaa
    18121 tcattttacg ctacgcagaa aaatgggatt tagaacctgc ttttgttgag tggttagaaa
    18181 aagaaaatgt tttctgttgt agtttagttg atcgcatcgt cccaggttat cctcgcgaag
    18241 atgcaggtgt tttcaatcaa cggcataact atgaagatca attgatggtc aaagcagaac
    18301 cattcatgct ttgggtgatc gaagggccta aagaattgga gaatcaacta ccattcaaac
    18361 aggctggctt gaatgtcatc atcacagatg atatgactcc ttatcgtgaa cgtaaggtgc
    18421 atttgcttaa tggaccgcat acagcgatgg tccctcttgc tttgttggcg gaattggaaa
    18481 ctgtagaaga agtaatgaaa gatcctttat tctccgcata tgttgatcag ttattcaatc
    18541 tagaattgat tcctatgctt tctttaccta aagatgaatt agccatttat gctgaccaaa
    18601 tcaaagaacg tttcttgaat ccttttgccc atcacaaatt agaagcgatt tcattaaata
    18661 gtgtttcaaa gttcagcacc cgtctgcttc cagtgttcaa gaagtatatc gaagaacaaa
    18721 atcaggtacc accgttaatc actgtttcac tagctgcgct acttcttatg tatcgaggcg
    18781 atcaagtaaa gccacatgat gatgaaaaga cgataagcga atttactgat gcttggtctg
    18841 ataatggaac agctattcct cgattattgc aaaacgctgc gctttgggga gaagatttgt
    18901 ctcaaatacc gaatgtgaca gatacagttc aagaaatagc tgcaaagatc gatcaaaaag
    18961 gtgtccggtc agtattacaa gaaatgaaag gaacgatggt ccatgcttga aaccctgcaa
    19021 ccaacaatga aattaaatcg acgagacagt gtggtcgtcg cattaacccc tattccaaaa
    19081 caaacacaac tgacgattga tcagcaaacg atcaccactc ttgaagatat cccgcaaggt
    19141 cataagattg cattgggtga tttgaaagct ggagacaacg tgatcaaata tggctatcca
    19201 atcggtcatg tgacgacaga cgtaactgcc ggtcaatggc tgcacactca taatgtaaaa
    19261 accaatcttt ctggtgaatt ggattatcga tatgaaaaag atgtccatcc aagtcattac
    19321 ctttttgaga accgaacatt tcaaggttac ttgcgaaaaa atggaaaagt agggattcgt
    19381 aatgatttgt ttttagtacc taccgttggg tgtgtgaatg gcattgctga actgatcgtc
    19441 aaacagttca aggaaaaaca tccagatctg ggacagtttg accatattac gattttaaag
    19501 catccttatg gctgttcgca gcttggaaaa gatcatcaaa atactcgaga aattttagcg
    19561 gacgcagtaa atcatcccaa tgccggagga gttttagtat ttggattagg ttgtgagaac
    19621 aatacagttc ctgaattcaa aaaaatttta ggtgaatatg atgaagaacg agtgaaattt
    19681 ctagtggctc aagatgttta cgatgagatc gaacaaggtg tggctctatt agaagagctt
    19741 ttaacagcgg cggaaaatga ccagcgcata tcagtcccgc tctctaagtt gaatgtcggt
    19801 ttgaaatgcg gcggttctga tggtctatcg ggaataactg ctaatccttt acttggtgca
    19861 ttttctgact acttgattgc tcaaggaggc agcacgatct taacagaagt gccagaaatg
    19921 tttggcgcag aacaagtatt gatggcacga gcggaaaatg aagaagtttt tgactcaatc
    19981 gttgacttga tcaatgactt caaacagtat ttcttgtctt acggggaacc agtttacgaa
    20041 aacccatctc ctggtaataa agcaggtggg atcactacat tagaagataa gtctttaggc
    20101 tgcacgcaaa aatcaggaag ctctcctgtc gttgatgtgc tacaatatgg cgaaaaaatc
    20161 agaaaaccag ggcttagttt attgcaagct cccgggaatg atcttgtagc tgcctctgct
    20221 cttgcttcat ccgattgcca gttggtcttg tttaccacag gaagagggac tccgtttggc
    20281 tcatatgtcc caacggtcaa ggtatctacg aatacaactc tttttgaacg caaaggtcat
    20341 tggatggatt ttaatgcagg cgtcttatta gaacaaccaa tggaagtaat cttagaatcg
    20401 tttgttcaaa aaatcatcgc tgtagctagt ggagaagaaa cgaaaaatga acaaaatgac
    20461 gttcgagaaa ttgccatttt taaaaacgga gtaacacttt gattttcata aaacagtata
    20521 aaaagaaaga aggaagattt atgtttttat ctgatgattt tttattaaaa gacgattggg
    20581 cgaaaaagct ttaccattcc tacgcaaaaa acatgcccat tattgattat cactgtcact
    20641 tagatccaaa agaaatttac gaaaacaaca atttcagcaa tctgacagaa gcttggttag
    20701 gaggcgatca ttacaaatgg cgcttgatgc gagcttgcgg cgtgcctgaa gaaaaaatca
    20761 caggaaatgc ttctgatttt gaaaaatttt tagcttggtg tcaaaccgta ccaaaaatta
    20821 tcggaaatcc gctttacagt tggtcacatc ttgaattgaa gcgtttcttc ggtattgact
    20881 tgttgataaa tgaagaaaat gcagagaaaa tttggacaca agcaaatgaa attttggcag
    20941 ctcctgattt tcgccgtcgg gaaatcgcaa aaaaatcgaa tgtagaagtg atttgtacaa
    21001 cagatgatcc cgcagacgac ttgcactatc atcagttgct tgctcatgaa gaaccagatt
    21061 tgaaggttct accttcattc cgtccagata aagcactcaa tattgaaaaa gaaggattta
    21121 atgcatgggt cacactactg gaagaagcag ctgaccaaac gatcaccacc tatcaagaac
    21181 tgatagaagt cttagggcag cggatagaat acttccatca acatggatgt cgtgtttccg
    21241 accacgcttt agacatctta cgctataaag aagcggatga aacagtatta gaagagattt
    21301 ttcaaaaagc acgagctaac aaactattat cttcagatga aatcgatgct tatcttacag
    21361 aaacactgat acatttgacc cacttctatc attctcataa ttggacgatg cagctgcaca
    21421 tccatgctta tcgtaactgt aatacacaaa tgttggaaaa attagggccc gataccggct
    21481 atgatggaat gaatgatctt tctttgacta ttcctctgca aaaattattg aaccgtgcag
    21541 aagaaacgga tcagttgcca aagacgatcc tttattcatt gaatccgaat gactatcctg
    21601 cgattttagc tttgatgggc tgtttccaaa aagaacagaa tggaaaactg cagttaggtt
    21661 ctggatggtg gtacaacgat actcgagctg gtatgcgcga tcaattgaca caatttgctg
    21721 atggtagtgc tttggggaat tttgtcggca tgctgacaga ttcaagaagt ttcctgtcct
    21781 acactagaca tgaatatttc cgtcgtgttc tttgtgaatt tttaggcgaa atggtcatgc
    21841 gaggagaagc acctgaagat gaacaacttt taggaagtct agttcaagcg attagttata
    21901 caaatgctaa aaattacttt ggcttttttg cctaattgat taatagtaac agtaacgaat
    21961 caaaaacagg atagtgaaat aacggaatcc gtttatccac gttttagaga agaagacgga
    22021 ccaa
//
LOCUS       DAPBCS010000050        20696 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPBCS010000050 DAPBCS010000000
VERSION     DAPBCS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.052"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            120..314
                     /locus_tag="RD521_002173"
     CDS             120..314
                     /locus_tag="RD521_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771880.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            583..882
                     /locus_tag="RD521_002174"
     CDS             583..882
                     /locus_tag="RD521_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771881.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(979..1413)
                     /locus_tag="RD521_002175"
     CDS             complement(979..1413)
                     /locus_tag="RD521_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771882.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1645..2355)
                     /locus_tag="RD521_002176"
     CDS             complement(1645..2355)
                     /locus_tag="RD521_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDT7771883.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2345..3205)
                     /locus_tag="RD521_002177"
     CDS             complement(2345..3205)
                     /locus_tag="RD521_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDT7771884.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3210..3848)
                     /locus_tag="RD521_002178"
     CDS             complement(3210..3848)
                     /locus_tag="RD521_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDT7771885.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3863..4162)
                     /locus_tag="RD521_002179"
     CDS             complement(3863..4162)
                     /locus_tag="RD521_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7771886.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(4205..5689)
                     /locus_tag="RD521_002180"
     CDS             complement(4205..5689)
                     /locus_tag="RD521_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDT7771887.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(5709..6170)
                     /locus_tag="RD521_002181"
     CDS             complement(5709..6170)
                     /locus_tag="RD521_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7771888.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6188..7255)
                     /gene="ulaG"
                     /locus_tag="RD521_002182"
     CDS             complement(6188..7255)
                     /gene="ulaG"
                     /locus_tag="RD521_002182"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDT7771889.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7473..8243
                     /locus_tag="RD521_002183"
     CDS             7473..8243
                     /locus_tag="RD521_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7771890.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8516..9343)
                     /locus_tag="RD521_002184"
     CDS             complement(8516..9343)
                     /locus_tag="RD521_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7771891.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9910..11145
                     /locus_tag="RD521_002185"
     CDS             9910..11145
                     /locus_tag="RD521_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDT7771892.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12144..12404)
                     /locus_tag="RD521_002186"
     CDS             complement(12144..12404)
                     /locus_tag="RD521_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDT7771893.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12431..13732)
                     /locus_tag="RD521_002187"
     CDS             complement(12431..13732)
                     /locus_tag="RD521_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7771894.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14024..14482)
                     /locus_tag="RD521_002188"
     CDS             complement(14024..14482)
                     /locus_tag="RD521_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7771895.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14495..15825)
                     /locus_tag="RD521_002189"
                     /pseudo
     CDS             complement(14495..15825)
                     /locus_tag="RD521_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(15836..17278)
                     /locus_tag="RD521_002190"
     CDS             complement(15836..17278)
                     /locus_tag="RD521_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7771896.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17265..18029)
                     /locus_tag="RD521_002191"
     CDS             complement(17265..18029)
                     /locus_tag="RD521_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDT7771897.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18133..19008)
                     /locus_tag="RD521_002192"
     CDS             complement(18133..19008)
                     /locus_tag="RD521_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7771898.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19263..19347)
                     /locus_tag="RD521_002193"
     tRNA            complement(19263..19347)
                     /locus_tag="RD521_002193"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19311..19313),aa:Leu,seq:caa)
     gene            complement(19365..19436)
                     /locus_tag="RD521_002194"
     tRNA            complement(19365..19436)
                     /locus_tag="RD521_002194"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19402..19404),aa:Gln,seq:ttg)
     gene            complement(19446..19518)
                     /locus_tag="RD521_002195"
     tRNA            complement(19446..19518)
                     /locus_tag="RD521_002195"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19483..19485),aa:His,seq:gtg)
     gene            complement(19580..19664)
                     /locus_tag="RD521_002196"
     tRNA            complement(19580..19664)
                     /locus_tag="RD521_002196"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19628..19630),aa:Leu,seq:caa)
     gene            complement(19685..19755)
                     /locus_tag="RD521_002197"
     tRNA            complement(19685..19755)
                     /locus_tag="RD521_002197"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19721..19723),aa:Cys,seq:gca)
     gene            complement(19801..19872)
                     /locus_tag="RD521_002198"
     tRNA            complement(19801..19872)
                     /locus_tag="RD521_002198"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19838..19840),aa:Gln,seq:ttg)
     gene            complement(19884..19956)
                     /locus_tag="RD521_002199"
     tRNA            complement(19884..19956)
                     /locus_tag="RD521_002199"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19921..19923),aa:His,seq:gtg)
     gene            complement(19959..20032)
                     /locus_tag="RD521_002200"
     tRNA            complement(19959..20032)
                     /locus_tag="RD521_002200"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19998..20000),aa:Trp,seq:cca)
     gene            complement(20039..20119)
                     /locus_tag="RD521_002201"
     tRNA            complement(20039..20119)
                     /locus_tag="RD521_002201"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20083..20085),aa:Tyr,seq:gta)
     gene            complement(20121..20196)
                     /locus_tag="RD521_002202"
     tRNA            complement(20121..20196)
                     /locus_tag="RD521_002202"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20161..20163),aa:Phe,seq:gaa)
     gene            complement(20232..20304)
                     /locus_tag="RD521_002203"
     tRNA            complement(20232..20304)
                     /locus_tag="RD521_002203"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20269..20271),aa:Asp,seq:gtc)
     gene            complement(20314..20386)
                     /locus_tag="RD521_002204"
     tRNA            complement(20314..20386)
                     /locus_tag="RD521_002204"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20351..20353),aa:Val,seq:tac)
     gene            complement(20402..20473)
                     /locus_tag="RD521_002205"
     tRNA            complement(20402..20473)
                     /locus_tag="RD521_002205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20438..20440),aa:Glu,seq:ttc)
     gene            complement(20488..20576)
                     /locus_tag="RD521_002206"
     tRNA            complement(20488..20576)
                     /locus_tag="RD521_002206"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20540..20542),aa:Ser,seq:gga)
     gene            complement(20616..20689)
                     /locus_tag="RD521_002207"
     tRNA            complement(20616..20689)
                     /locus_tag="RD521_002207"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20653..20655),aa:Asn,seq:gtt)
BASE COUNT         6534 a         4337 c         3292 g         6533 t
ORIGIN      
        1 gagtgaaaaa aactttcaaa ttgattcttt ttatttatca taaagatata gatacataat
       61 cattttcact tacatatatt tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa
      121 tgtattatgt agaagtaaaa acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca
      181 gtaacgaata tcctttactt ggttcatgga aagaagctgc tcctttttcc aaaccatgtg
      241 ccataaaaat aaagaacgag ctagaaaaag aactgacttg tggcaaagct gttgtagata
      301 tcatcgaaaa gtaaatccaa tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa
      361 attgctaaga ttatagaatt agaagaagaa gacgcagtgt tgacaattga aaaaaaataa
      421 atgtttgtat accatatgat tatttcaata tgtatcctgg aattggagat ctcgtagagc
      481 tatatcaaga tgaatcaaat atacttgtca ttcatcatga aatatagaga cttataaaat
      541 tgtttatttt tataatgtat cagtatagaa aggaaataaa acatggatta tactgttgaa
      601 tttgacagtg aactacttaa gcaatttcca caagaagaac atttcatgta taaagaagca
      661 atttcccaca aagtgacaga gtatttggag cataattttt ttcgtataaa gccagtgcgt
      721 actcgttcgg caaaaggaat atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc
      781 atagcatttg ctttaaaaaa aaatcatgtg ttcatttttt atattagttc gaatttgcaa
      841 aaaattgtat ttgataaaga ggtgagtaaa aaaataaaat agctgagcat tggaggatag
      901 attgagtgat actaagtcta tcctcttgtt tttctaaaga actaagctag aattttttct
      961 tttgtacgtt attatatgtt atttgtcatt accaaaaagc accttttttc ttctagtatc
     1021 tatgacataa gaaataccac ccatttcctt tacttgaaca aatcctactg attcataaaa
     1081 tttttttgtt ttctcagtat catctgtcaa taattcgatt tggactggtc cataactagt
     1141 gatcttctcc aaagcttcat tcaaaagcac tttgcctatt ccttggcgct gatacctcgg
     1201 atgaatgagg atatcttgaa taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat
     1261 tccgataatc ttcccttccc cattacgctt tatcagagat tcagattgtt caaatgctct
     1321 agctaacctt gcagtgtctt ttgtgtaatt cgtccaacca acgctcttat ataattcaga
     1381 aatctcatcg atcataaggg aatcaatatt catttttttc ttcctttctt atacaattct
     1441 ccctctaata atacagttct ttgactattt aatctatcac atataactct cctgtaaaga
     1501 ttctctcgat tccttaaaat ctctctttcc ttttagaatg catccttttt gataaataaa
     1561 aaggttgtga taaattttct tcacaacctc tgtttctctc tatcaagtgt tatcttttaa
     1621 ttttctaccc ttaccctagc atctctattg cccgtagtat gcatactttc catgtttgcg
     1681 aaaataatgt ttatctagta aatattgcgg gatttcacat atattggggt tgatcagctc
     1741 gttttctttg gccatcagac aaatttcatc taagatcaaa ctgttttcta ctgctttcat
     1801 aggtgaatcc ccccatgtga acggtccatg tccatagaca agtactccag ggacttcgtt
     1861 tggatctaat tttctttcct taaacgtctc gacaatcaca ttccctgtat gaacttcgta
     1921 agcttctctt acttcttctt tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc
     1981 atcagcatga gtcgtgccat aagctggcaa atcgcgtcct gcttgcgccc acatgacgga
     2041 gtgcgtggaa tgagtatgag taatagcagt tatatcttca aaagtttgat acaaaacaac
     2101 atgtgtgggg agatctgaag atggttttaa gctatcttct tctattatat ttcccgacaa
     2161 atctgttacc accatttgat ctgcttgcat gcattcatat ctgacaccgc ttggcttaat
     2221 gacgatgact cctagctcac ggtccacttc actcacattg ccccaagtca atttgactaa
     2281 tccgtatcgc ggcaatgcca gatttgcttc aaggacacgt tgcttcattg ctgcaatttt
     2341 ttcttcacaa gacatcatag ccagcctcct tcaatttaga acttaagtag tcttttgcct
     2401 gctggatttc tcttcgaaag tcttggcttt tttcactcca catttcaatc agaaatgttc
     2461 catcataatc tagacgtttc aaatgtctta ataatccaag aaaatcaaca caaccgctac
     2521 caaaaggaac atcacgaaac tggcctgaat attcttttgt tacagggtaa gtatctttta
     2581 agtggatagc agtgatataa tcgatccctt tctcaagttc tcgagccgga ttattttccg
     2641 gccatgcgga caaattgcct aggtctggat atacttgtag aaaaggggaa tgaatctgtt
     2701 ctttgatttc taaaaatttt gaaatggagt tcataaatgg atcatccatg atttccacgg
     2761 aaagaatgat gccatattta gaagcttcct ttacgctttc ttttattcct tcgataaagt
     2821 cttctctgga attcattgat ttctcttcat aataaacatc gtatccagct atttggatta
     2881 ctttgacgct tagattatgc gccaaatgga ttgctttttt cattagttgt ttcgctgctt
     2941 ttttcttttc tggatcagca gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg
     3001 aatggatacg tacaccggag ctgaacatct cttctctcag ctcagctatc ttttcttcag
     3061 accaatctaa tcgtgctaac cgctcatccg tctcatcaat cgacatctcg ataaaatcga
     3121 agtccatttc ttttacaagg gaaaaacgtt ctttccatga aatatctttg ggtaatgctt
     3181 tttcataaat cccaatttga gccatttcct tacccccaga tacgaatgat ctcatctttg
     3241 aagttttgtg cggccttcat cggatcattt gctgctgtaa taccacgacc agtgataaag
     3301 gtatacacat cgatcccttc aaataagtgt aatgtgtgtg gatttaatcc accagttaca
     3361 gaaacattaa agcctagttc aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa
     3421 gtttcaccag ataacagcgc atcacggctt tgatggtaga ttacctgccg gataccgatg
     3481 ttatgccaat ccatggcttg ttcaaaagtc caattgccgt agagttctac ttgcaattca
     3541 cctatttctt tttgtgctgc ctccatagtt ggaatcgttg cacaacaaat gactgtcatc
     3601 cagtctgctc ctgcttttgc aacattactc gccactgttc ctccagcgtc tgcacatttc
     3661 gtatcagcga ctagtttttt atctgggaac atactgcgaa tacagcgaat cgctttttgt
     3721 ccttcctgta agcaaagaat tgtgcccact tcaataatat ctactacatc accaactttc
     3781 ataacatctc ctaatgcatg ttctaagtca ttgtgatcta acgcaatttg caaattcggc
     3841 ctgctcatct tagttccttc ctttacttgt ttttttggta ccactttgtt tcagcaaatt
     3901 tttcactgat ttctttggct gacatcacat ttttgatacc gataactgta acttcttttt
     3961 tctctgcctg agtaaacata ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc
     4021 cagctgaact ttttccctct gaaatggcac aatgatggat tttggtaatt ggtacgttta
     4081 gttctttcat tgctttttcc acacttctca tcatcatcaa gcttgttcct gatccatttg
     4141 cacacgatac taaaattctc atcttaatcc ctccattatg agtgttgtct aatcagattt
     4201 tcttttatac tctctgcttt tctgcatatg tgtcatagtc ttctacaatc aaaaaataac
     4261 cctcaggatt tttccgatat tgtaactgcg gaatagccaa taaaccaatg acaactacgg
     4321 caattccagc ataacctagg actttcatga ttactgtaaa caatggccaa gctgttgccc
     4381 agtcgaacat cccgatataa ccaccatatg ttgacatccc aatccatgta gcgaataatg
     4441 ctgaaccgcc tacttgtatg attcctgaaa gaaacgggaa caggcaagcg gctttgaatc
     4501 cacctcggtt attggcataa actgctataa cggcattatc aaagaacaac ggaatgaaac
     4561 cggctatgac gatcgttggt gatttcaata aaatcagtaa accaatcatc aaaaactgtc
     4621 ccaaagcacc aaacaagaac ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag
     4681 cagctacgtc aattcctgga acagctcctg gtaaaattgt gtttgagatt ccttggaaag
     4741 attgagttag ttcatcgacg aatgtccgaa cacctaattg caagatcgcc agatataccg
     4801 caaaatttaa agaagtcgtc ataatgtaga ataagaaact ctgtccttct tgcatgaatt
     4861 gcgcttgaat caaataatct ttgcctagta caatcagaat gatcccaaag aaaaatagca
     4921 tgagtaacga agtagcaacc atattctcat tgaaaatcga caagaatcct ggtaatttga
     4981 tatcttcgat ttttttgtct tgcttgattg atctattttt cttttcatcc cgctttttca
     5041 tccactcagc taattttgca aatataaaga ttccgaacat ttgctgatgt gccactgcaa
     5101 atccggcacc ttctgtcaat tcctgcgtga ttcctactgt taagttagaa gctactgccc
     5161 aataacaacc taaaatcagt cccataaaaa tcaatacttg tatcctacct agatcaggaa
     5221 agcaaaacag taaaatccaa aaagcggtag ctgcttgttg aacctgaacg tttccggtag
     5281 tgaaaacgga acgcagtttc gtatatttct gaaaacggac taataagata tttaaaacaa
     5341 atgcaatcag taataaaatc atcacatctc caaaggtacg gccaaatgtc tccattaatc
     5401 cagcatccac agcattttgt ccaaaataag gatctgtaac catggcatcc aaattgaacc
     5461 gttcctttag tccaaccaaa atcggccgaa aattattgac caaaccacca gagccaacag
     5521 tcaaaataaa gtaaccgacc gttgccttta agaatccagc gatgcattca tgaagcgggc
     5581 gtttcagcaa aatatagcct aataacacga taaaaccaat caggtatgct ggctgcgtca
     5641 agatatttgt tgcaaaatac gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc
     5701 tctatcaatt aattatattc tgccatcaca acttggtaat catcgatgga gttcgtagag
     5761 atgagacgct caaccaatcc atctgtcatt aataattcgg ataatttttg gatattttct
     5821 agatgttctt ctggatcttt tgctgcaagt gtaaaaaaca aactagccgt tttctcctca
     5881 tcctcccctt cactttcaaa acggattggc tgtttcaact tggtaaatga aatagctgtt
     5941 ccaaaaacac catcactttt ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca
     6001 atataaggac cgtatttttc tacacaatgg atgatctcct ccacatacgt ttcatcaata
     6061 atttccttat cgatcaatac aagacagctt tcggcgatag cttcacgcca atccgtgatt
     6121 tctttatctg ggtaatgaat caattgatgg tcaaaaaagt atttcagcaa gaaactcccc
     6181 ttctttctta taaaaacgac ggaaacggtg tatcattttc aatcgagaag acatcttcaa
     6241 atcctcgata aaaattaaac tcaagatcat ctttgtttgt cggatagatg aatttcccgc
     6301 ctacttgcca gatataaggt ttaaatccat acttcaggcg atcctttttg aatttccaaa
     6361 tttcagtgat ctcttttgaa tcagccataa aattagacca aatatcgtag tggacaggaa
     6421 tcactacttt tgcattcaat gactccgcca tgcgcagcat atcaacagaa gtaactttgt
     6481 ctgttatccc tcgtgggttt tcaccatagg cccctaaaca gacatcgatt ttgtgttcat
     6541 taccatgttt ggcaaataaa ttggagtaat gtgaatctcc tgcatgataa agattccctc
     6601 ctgatgtttc gaataaatag tttactgcaa tttgatccat ctcctgaggc atttttcctt
     6661 ttaatacctc ttctggatct tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag
     6721 ctactacatt gatgtctttg attttgactg catcacctgg ctttactgta atcgtttttt
     6781 cttcaggaat tccccatttt tgccacgtgc gaaccacttc ttttggccca atgaatgttg
     6841 cttcaggaca attttgatga actgctgcgg ctgtatggat atctagatga tcggaatgga
     6901 tatgtgtaac gattaaagcg tctacatttt ttatcgcaaa aggatcgatt acgaaaggct
     6961 gagtacgtaa attaggctgc atattttgaa cgccactcat acgcatcatc tgatggcctt
     7021 ttttcatcat gccattccca tgactacgtt ttcccgtccc acaccataga tcacataaaa
     7081 tattcgttcc ttcatgactt ttcagccaaa ttcccgtaca gcccagccac cacatagcaa
     7141 aagttccagg atccactact tctgtttcga tttcttcatt caagtatgtg ccccactcag
     7201 gaaatgtttc tactaaccac ttttcttttg ttacttcatt gacagtttta ctcatttgct
     7261 tgctcccccc atatcgttgt tccaacttac aagtaaatat taatgtgtat cagcatccat
     7321 ggaaaccctt ttatatgacc gtttgttttt tatatgattt tctttcagta atcaatgatt
     7381 gtttcagtat aataaagaca gatatgaatg tttaaatgaa caactagtca aataaatcca
     7441 tggatcaaga tgtccctcag gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa
     7501 aacgtcaaaa agaaattttg gaactgcttc agcagactga acaactatca actttcgaat
     7561 tagctgaatc ccttgatgta tctattagta cgatacgaag agatttaaat ttattagaag
     7621 aaaaaaacga gatcatacgc aattacggtt attgttctta taattacaaa aaccagactg
     7681 attttgatca atctggtcct gaaaggatta aacaagcgat tgccagagaa gctagtaaat
     7741 acattaacga ttatgatacg ctgtttatca attctagctc gactgcgttg aaaacactgg
     7801 attttcttca atctaaccat ttgacagtca ttacaaacaa cgtgaaaatc aattactctc
     7861 cgcacaaaga taattgtagt tatattttaa ctggtggaga gatgcgcttt ccaaaagaag
     7921 cactagtggg cgacatcgca ttaaatacga tttcttcagt caatgcagac atttgtatca
     7981 ttggctgtag cggagtcgat gtagtacatg gggtcacaac aaagatatta aatgaatcaa
     8041 cgatcaacga aatgatggtc aacaagacaa tcaaatgtaa gattttagtc gctgatcatc
     8101 gaaaaatcgg actgacatca aagttcaaga ttgctgatat ttcagtcttt gattatttga
     8161 tcactgataa attttgttct gaaaaagtat tagaggagat cagaaatacg gggataaaaa
     8221 ccattcaagt agattcattt taagaaccag gacgtaaata tgtaaatagg attatcaaaa
     8281 aaatgaataa caaatttatc gataaatgaa aaacacgcta cgctcaaatt gataaaggaa
     8341 tcccaagatg tgtccatcca atcgatacca tcataatcta ttgcttcttt tataaccgtt
     8401 atatgttgcc taaataggaa tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca
     8461 cctccttttt tcgaataaac ggtattcaaa tcagaacact gatttcacaa ccactttatt
     8521 tcaggtcgtt ttcaatcacc tttaccgcgt aatcatttcc gttgaaacgc gctatttttt
     8581 ttgcgtccat atatctttct aaataatcat cttctcctaa ttgcttatgg caagaagcaa
     8641 tttcataaag caagtattcc aatgcataag tcgatttatg tttcttatta tactcaatac
     8701 ccttctcaca taattggatt gctttatcat aatcctcgat tttacggtaa taggtggcga
     8761 tatcataata tacaatgcag accatcagat tatcaaaatc agtctcactc aatattttaa
     8821 gtgtacgctc cacataaact ttcgcaaaat ctaattctcc ttttgacaag tagatcattg
     8881 ccacgtttgc agctgcttgt aagtgatgga tatctgtttc ggtcgtttgt tccaacactt
     8941 gattaaaata aaataatgct gcatctggtt ttttcgcata aagatgatca agcagtcctt
     9001 gataaaataa aatttgattt aaaagatcag aattacttac tttttcctga tcgatcgttt
     9061 gtaaaatttt ataagattct tggtattttg atacagaatt caatgattga atctcattta
     9121 tttttttcct gattttgtct tcttctgttt ctactaatat atcggagatc tcgacattca
     9181 gctttttgca aagcgcactt aagatattga tgcctggcga catattcttg ttttcgatat
     9241 tgctgacagt tgcttgttca caaatgcctt ctgctaattg tacttgtgtt aacccctttt
     9301 tctttcggta ttcttttagt aatgctccat ctactttaat caaaacaatt tccttccctt
     9361 taaaaaatta tttctccttt atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt
     9421 ttagaacaga ataaaaattt taattttaaa ataatattca aattaaacaa ctggcgtatc
     9481 tgctccttga tttctttttg tgttatctga ataattatcg tttacagtta taacacatag
     9541 tgaaaattgt catggcgaga aagtctaaga atatttttaa acataactat ttctttctta
     9601 cgtgtgtata atgacgacag cacacctatc aattaatata attattttat tttttatgtt
     9661 ctcatgttta tttttaatga cacaacttct cgtatttatc tggcacaaca tataaatcga
     9721 tcaaaaaatt gttgcgtgtt tttttgaaaa ttggagtttc aacttgatta caaaatttaa
     9781 atttagaagt tgtgaaaaca gcgagagaca gtttaatatt caaaatatac ataggctact
     9841 cgaacgctgt gttttttagt atactagtaa gaaacgaagg gatagactca ttccaataag
     9901 gaggatttca tgaagacaat tctttttgct cctgcagtat tcaatttagc tgagacaaca
     9961 cgaatgattg aggttgccaa agctatgaaa gaccgttatt actgtgtatt ctttggcttt
    10021 tctaaaactt ttgctccttt gatcacagaa agtggctttg agtatcattt actagcgccg
    10081 atccttacga aaaaacaaga agaacaaatc ataaaatttg accagttgcg tacacttaaa
    10141 aaccctttta ccaaagaaat ggttgcctct cgtatcacta gtgaacagag tctgattgct
    10201 aagtttgagc ctcgactgat tgtgacaggt tcgaatgtga caattttttt atcggcccgc
    10261 aataaaaaga ttcctttagt atatgttaaa ccctatgctt tgagtgaacc tttttttcaa
    10321 tcagagcatg cgctgatgcc ccacgagtta cgtttcttcg gccctttaag caatcccctt
    10381 tggcactgga caaaaaagcg tatagtaaat acccgatgga tccccaatgc cttcaaacac
    10441 gctgcacaaa aagaaatgct tcctttacca aaaacaagtt tacaaatgat ggatgctgat
    10501 ctaaatctca tcacaacgcc taaactattt ttggaagaag aacagctgcc ggaaaattat
    10561 cgaatcgttg gtccaatttt tgctaagctc agtacttcct tgcctgaaga agtgctaact
    10621 tttatacagc gaaagcaaca agaaagaaaa cgtattatct atttcgcaat gggtagttca
    10681 ggcaatccgc gtatgttacg caaaattttg ttcttcttac aaaaacaacc ggatctagcg
    10741 gttattgcac ctattgacta tctgcttgat gacattcaat ctgactcaga agatctttta
    10801 ataacagaat atctgtctag tcatcttttg catgaattca ttgatttttc ttttatccac
    10861 ggcggggaag ggaccgtcca aactgcctgt gaatccggca aaccgtttat cggtgtgggg
    10921 atgcaatttg agcaaagctg taatattgac tattgtgtaa aatacggaaa cgcaatcgcc
    10981 ctcacaaaac cagtgactga aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc
    11041 aagattcgtc agcaggcacg cacgctgcaa gccgtttttc caataaatgg tccacaacaa
    11101 gcaattcaag aaatcgaaac cttcctgagc aaccacccaa tatagtttca agtagaaaaa
    11161 gcttttttaa tagagactca tcagtaattt cgctattcga gacccagaca gtgaaaatac
    11221 aaaaaaagta aatagtggat tgaaaagtta gagttactta aattacggtg tctaaaaaac
    11281 acaaaatcat ccttaaaata aaagatctga cgcaatccaa atgagcatag gtagcgtctg
    11341 ccatttattc ttagtcaaat tctttgacac ctttctctgg ttggatcatc tcaacgtttc
    11401 ttcaaaagaa agaccataat aattaggcaa atacattttc ttaaaatgtg tcatatagat
    11461 ctagaagcat cgctttgtag agatttgtgc tgctagtccc ttcaacaatt actaaatcaa
    11521 atgtaggatt taccacttta tctttgataa tcaacctccc tcttctgttg agacaatgat
    11581 ctcgcaatcc gatgcatact cctatataaa ctgccagcaa tccccacatg ctgagtagat
    11641 catgtcagaa tgttctatgt cgtcagcaac gataaactaa aagtcacgtt caccatgact
    11701 gattgctatt cccagcgcaa ttatttctat acacgtttca tgatttgaat aaacactctc
    11761 atcatacata ttaccattca tacaccatac tgcagcccct actatatggt tgtaagctcc
    11821 caaatctata tttgccttga tcactttggt agtagatcga ctaattctta atccattgct
    11881 gataaaattt tcatttttgc ctccttcttt ctgccaaaac agttaaaaca ataataatct
    11941 ttatgtaaaa aagggctgtg acaaaagcct tgtcacagtc ccatattccg aataaacggt
    12001 ggtcaaaagc ttgcatctgc ggtaataagt tcaaccaaac gaaaaataag ccaacatttc
    12061 acaaaaattt aagaagcaat tttcgtaaat ttcttcttat ttcttgaagc taaccacttc
    12121 tgtcacaacc acttttagaa catttaaagc ttcgatgtga caaaccatct atgctgctcg
    12181 atcataaaat tgatagcaaa aattccacaa agtacatatg tcccccatga aaacaaaaca
    12241 ttcatatgcg gtacaatgat ctttggaaga atcacgatca aaaatgatgc cagtgcccaa
    12301 atccatcgat tgatttgaat atgatatgta gctagcatat tcaatataag gtgactagcc
    12361 gttaataaaa agataaaaac aaaaaataaa atcagccaat acattgtttc ccaccttcta
    12421 aaaaccaaac ttagacagtt tggacttcgt tttctccacg actttgttca tctgacagca
    12481 tctttttatc ataagctttt acaaatggat aatagataat aaatgctatc aatgtgttga
    12541 tcatgatcaa gataacaaca cgccagtcat ttcctgctga aagataaccg ccgattggag
    12601 ctggtaatgt ccaagttggc aaaacagtaa atctattgac catttctaat ttgactgcaa
    12661 aataactgat aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac atgtaaggat
    12721 tcaacatcat aggagctcca aaaatcatgg gctcattgat attgaagata ccaggaataa
    12781 tagaaaaacg accaacctct ttcaagaatt ttgatttaca gaagaatata aacaaaacag
    12841 ataccacgat cgttgctcct gcaccaccaa ttgttaccgt ccattgataa aactgttcag
    12901 gagcgatata gggtaattgg gtggcagctg taccatctgc caatgccttt gcattctcat
    12961 ccaacatgat cagccacatt ggacgaacaa cagcccctac aatactcatg ccatggatac
    13021 caaatgacca caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc aaattattcc
    13081 ctaaaatatt ggttaaaggc tcgaataacg ataataaggt agcgtttaca tcaaagcccg
    13141 cgacgcttcg aatcagccaa gtaattgtta aaatgactgc acctggaatc aatgcctcaa
    13201 aactacgggc aacagcaggt ggcacttgtt ctggcatttt gatagtaata tttcgatttt
    13261 taaagaaacg atatacttcc actgcaaaaa tcatagataa gatccccgca aacatcccag
    13321 atgctcccaa agaactcata ggtaagaccc agccaagagg tgccttttca ggagttaaaa
    13381 cgtttacagg aacttgtaac ataaagaacg ttcctaaaga taatatacca cctgtcacac
    13441 tatctaatcc atatgattta gctagatccg aacccataac aaaagttgca taaatagcca
    13501 tcaatcccat cgtcatccta aaaggtaaaa caatcgttgc cgaataaggg gcaataaatt
    13561 cattccatcc aggaaacggt aaattgataa agaccataaa aaatgtccca atcaaaatca
    13621 atgacaatgt tgaaacaacc ccattacgaa ttgctaataa atgacgctgt gtaccaattt
    13681 ttgccattgg tgggacaagt ttctcttcga cccatttcat cagcttttcc actaaaacca
    13741 ctccttttca ataaaatatg aaccattcat tgcatgacaa cgcaaagaat gttcataact
    13801 aattatataa ttaagaaatt ttatttcaat atattctctt aaataatcaa atttattttt
    13861 aagaatcctg aaaataagtt ttctaaatca taggagcaac aaaaaaattc gataattaac
    13921 aaaaatcaaa atgattaaat ctagtgattg aaacttatca tttcaaaaaa attttgtaaa
    13981 ttcacccata aatatcaccc tatttaactt ttttcatcgt acactaaaat tcaaaacaac
    14041 tatctttaat ctttacttct tggttttgtt ttaataaatt tccttcaaaa atgcctggag
    14101 aaactacaat gatcaccgta tctttttgtg cttgctccaa cttcttcaaa tctataatag
    14161 ctaacagtgt cccttctttt acctcctggt tactttcaac ccgtatctga aacggacttc
    14221 cttcgagttc aactgtatct aaacccatat ggatcagtat ttttctgcca gattcacttt
    14281 cgatcgttat ggcatgtcgg gtaggaaata tattatcgat tattccttta actggcgcat
    14341 ggatatatcc atcatgattc gttatagcgt atccaactcc tatcattttt gaagaaaaga
    14401 cctcgtcttt tactgctgtt aatggaataa tttgaccact agctacagct tttagaatgt
    14461 ttcttgtttt aaagaaatgc actttttatc acctctactt taatgctggc caatattttt
    14521 tgtttacttt gatcatttca tctagtattt tttttgcgac tctggcattt ggtacggttt
    14581 gattcaatgt aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa gcatctacta
    14641 gtaacttctc tgctgcaact tgtgcctcca tcaatccttt atgaaaatca ctgattggtt
    14701 ttcttagtgt aattgtctcc acaccagttg caccaacata tgccggtatt tcaacaactg
    14761 catctgaccg tagattcgga atcgcccctt gattaggaac gattagcata aagcgacgac
    14821 gatcatcatt caacaaggaa atagctatat cgacgatata ttgtccatgc tccccaaaat
    14881 taaagttcaa tacttcttcc acaataccgc ggcgtatttt atcagccatt tctttggtat
    14941 ttttctctcg tccatccatg accatattag cacgtgtata ctctttgtca gtatgctcaa
    15001 cgaccatatc aggatataag taatactcta agtaattatt cgggatatta cttggaaaat
    15061 ttttcgtgat gactgacatc atttgaaagg ttttttgcca agttttgtct cctatattaa
    15121 aatcggcgac ttgcatttct tttgttatta gtttttcaat aatctctggc ataatttctc
    15181 gttgcaattc tttatcataa atcgatgtat accagccaaa gtggtttaaa ccataatacg
    15241 taacgatcca attcttacga tcataaccgt aattcactgc aatcgtttct tcaattgaaa
    15301 tagtcatatc acaggcatta atcattttta cattcggata tttacgacga acagcttcag
    15361 aaacgatcga ctctggattt gtataattca aaatccatgc tttgggggca tagtcttgta
    15421 tatgatcgac aatttccaat aaacctttga ttgagcgcat cccataagca aatcctccga
    15481 gtccacacgt ttcctgtcct actaatccat gtttaagtgg aatcttttcg tccttttctc
    15541 gcatctccaa tcctccaaca cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa
    15601 gctttggatc atccgttgca cgaatgcgaa tatttgaaaa accttttttt tcagcataaa
    15661 tttaacaatc aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc
    15721 agataatggc aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt
    15781 actcccgcct ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt
    15841 cttctaataa tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt
    15901 gataattttt ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt
    15961 cctcgaccat ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat
    16021 ccaggatatt ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat
    16081 ttttacttgc taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct
    16141 tagcttcacg tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt
    16201 aatacaaggc aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc
    16261 gaaaattgat gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga
    16321 ataagtaaca gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag
    16381 gagaaagaaa catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc
    16441 ctgcaccgct tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat
    16501 agatagctag acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa
    16561 acagtccagc ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct
    16621 tgctaggaga tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca
    16681 gtccaaaggg gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgccccca
    16741 tatcaacgag cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc
    16801 ccaaaaaaat agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc
    16861 ccgcaaaata agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg
    16921 ccgctactaa accggaaata attccaccaa aaatccccat attcacttct gggtccatga
    16981 tttctaagcc ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt
    17041 tgtccttatt tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc
    17101 caaaaacggc atttccagct gcaccaatta ctgggatgtt cagcatatca tcagctgata
    17161 aacgcatgat caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa
    17221 gcttctgcac atttgattta acattcattt ttattcctcc tctattaaat atcctcatat
    17281 gttactaact caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa
    17341 aattcgactt ggtgagtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc
    17401 aaatacgctg cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa
    17461 cttgccaaat attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct
    17521 acgacatcga aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag
    17581 ttgctccgaa ccggcaaatc atattttttt gctaatgtaa caactacctc ctggttttca
    17641 tccaaagtat gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa
    17701 attttttgaa tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga
    17761 tgctgataat aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg
    17821 agaagtggtt tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc
    17881 tttcttagtt tcacagcgtg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc
    17941 aaaattcctc gctgataagc atccataatc ccataattca caccatggct atagccaaaa
    18001 tcatccgaat taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag
    18061 gcgcaaccaa ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt
    18121 tacgccctta ttttaataga ttcgtgctag atgaattgcc aaatagatga tttcattttc
    18181 ttgaatggca agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata
    18241 cgctttgcta tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc
    18301 ctgataaatc aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag
    18361 tgattcttta tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt
    18421 catttctact atttttaagc tctttaaact attaggctct cctagctcag cattaatgat
    18481 atgcatagtg atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat
    18541 tcgctcgacc acttctattg ctatatcata ttcatttgga taaaggaatc tcatttctgg
    18601 caacataatt gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg
    18661 atctgtcaat gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag
    18721 ttcaatcact tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact
    18781 gaatcgtttg atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc
    18841 aaatggctta ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat
    18901 tccatcgtca tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa
    18961 ttcacctcca ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt
    19021 ctccaaacaa tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc
    19081 ctaattgaat agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg
    19141 ttatcaatat cttttttact cgttttatga taatacttgt atcgaattga tttggaggta
    19201 attacataaa aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat
    19261 tataccggcg gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag
    19321 cgcgtctgcc aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt
    19381 cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa
    19441 taaagggcga atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa
    19501 ccacttcgcc acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag
    19561 atgaagacat cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca
    19621 ctggattttg agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt
    19681 actaaggcgg taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca
    19741 cttggctata ccgccattat actattatca atctacaaaa taaagaataa attatagaaa
    19801 ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt
    19861 ggctataccc caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc
    19921 cacaatccgg tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga
    19981 acccacacta acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg
    20041 gaggagagtg gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta
    20101 gccacttcgc tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc
    20161 ttcaatccgt tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat
    20221 aacaagaacg acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca
    20281 tgttaacccc tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga
    20341 ccctctgctt gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa
    20401 atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct
    20461 tgaccaacgg gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt
    20521 ttgcaccatc tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag
    20581 tgaaagaagc tcaatactct ttctcaaaat gagaactccg gcagtaggac tcgaacctac
    20641 gacatcatga ttaacagtca tgcgctacta ccaactgagc tatgccggaa taacat
//
LOCUS       DAPBCS010000051        19639 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPBCS010000051 DAPBCS010000000
VERSION     DAPBCS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.053"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            127..3654
                     /locus_tag="RD521_002208"
     CDS             127..3654
                     /locus_tag="RD521_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDT7771899.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="RD521_002209"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="RD521_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDT7771900.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="RD521_002210"
     CDS             7442..7783
                     /locus_tag="RD521_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7771901.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="RD521_002211"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="RD521_002211"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDT7771902.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="RD521_002212"
     CDS             9140..11560
                     /locus_tag="RD521_002212"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDT7771903.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="RD521_002213"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="RD521_002213"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDT7771904.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="RD521_002214"
     CDS             12994..13731
                     /locus_tag="RD521_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDT7771905.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="RD521_002215"
     CDS             13745..14560
                     /locus_tag="RD521_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDT7771906.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="RD521_002216"
     CDS             14573..15220
                     /locus_tag="RD521_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDT7771907.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15891
                     /locus_tag="RD521_002217"
                     /pseudo
     CDS             15236..15891
                     /locus_tag="RD521_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            16053..16874
                     /gene="racE"
                     /locus_tag="RD521_002218"
     CDS             16053..16874
                     /gene="racE"
                     /locus_tag="RD521_002218"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDT7771908.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16877..18232
                     /gene="rph"
                     /locus_tag="RD521_002219"
     CDS             16877..18232
                     /gene="rph"
                     /locus_tag="RD521_002219"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDT7771909.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18233..18751
                     /locus_tag="RD521_002220"
     CDS             18233..18751
                     /locus_tag="RD521_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7771910.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18858..19358
                     /locus_tag="RD521_002221"
     CDS             18858..19358
                     /locus_tag="RD521_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7771911.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6587 a         3375 c         4214 g         5463 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gtacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaaagtcac aaccgctttt cctctttcca ttcgaaaaaa
    15961 agagatttcg attgttttaa gcaagtaaga gcacttgaaa attcacgcta aaaagtacac
    16021 aatagggagg atagaattta ttgaaaaaaa ggatgatacg attgacagat aatcgcccta
    16081 tcggatttat tgattcaggt gtcggcggct tgactgtagt aaaagaagcc ctgaaacaat
    16141 taccgaatga aaatatttta tttgtaggag acacagcacg ctgcccatat ggccctagac
    16201 ccgcggaaca ggtaatccag tatacttggg aaatgacgga ttatctggtg gagcaaggaa
    16261 tcaagatgct ggtgatcgcc tgcaataccg caactgcggt ggctttagaa gaaatcaaag
    16321 ctgctctttc tattccagtc atcggtgtga tccttcccgg tactagagcg gcagtaaaaa
    16381 aaacacaaaa taaacaagtt ggcattatcg gtacgattgg tacggtaaaa agtcaagctt
    16441 atgaaaaagc actgaaagag aaagtaccag aattgactgt gacaagtctt gcttgtccca
    16501 aatttgtttc agttgtcgaa agtaatgaat accattcatc ggtggcgaaa aaaattgtgg
    16561 cagaaacatt agctccttta accactaaaa aaatcgatac attgattttg ggatgcaccc
    16621 attatccatt attacgcccc atcattcaaa atgtaatggg agaaaatgtt caactgatcg
    16681 attctggagc agaaacagta ggtgaagtat ctatgctgtt agattatttc aatttgagca
    16741 attcaccgca aaatggtcgg acattatgcc agttttatac aactggctct gccaaacttt
    16801 tcgaggaaat agctgaagac tggcttggaa tcggacactt aaatgtagaa catatcgaat
    16861 tgggaggaaa ataaggatgc gtcatgacgg gagaaaagcc caagagcttc gcaaactgga
    16921 gcttcaaaca aatgttttca aatatccaga aggatcggta gtgatccgtt ttggtgatac
    16981 aacagttatt tgttctgcaa caatagaaaa ttctgttccc ccttttttga gagaaacagg
    17041 aacaggatgg gtgacagcag aatatagtat gctgcctcga gcaacgagta cgagaaatcg
    17101 tagagaaagc agcaaaggga aactttctgg tagaacaatg gaaatccagc gtcttattgg
    17161 tcgttccttg cgtgcagtag tagacttgga aaaattagga gaacgtagta tcatcgtaga
    17221 ctgcgatgtg atccaagcgg atggcggtac tagaacagca agtatcactg gggcatttgt
    17281 ggcattgaaa ttagccattg aaaaactttt aagagaaaaa gaattgtcag aagatccaat
    17341 aaaagaacat cttgctgcag ttagcgtagg aatcttacca gatgggactt gcgtcacaga
    17401 tttggattac caagaagatt ccgcagcact tgtagacatg aatcttgtaa tgacagaatc
    17461 tggaaaattc gtggagattc aaggaactgg agaagaagca acgtttgatg gtgaacagtt
    17521 gaatgaaatg ctcttttttg gaaaaaacgc gattgaagat ttgatcaaag agcaaaaaca
    17581 tgctttgcta acagagtttg ctcaaaatga tgagcggata gaagaaacaa aaacaatcat
    17641 cattgcgaca agaaatccag gaaaagcaga agagtttcga aatatgttca aagaagcggg
    17701 atatcatgta aaaacattac ttgactatcc tgaattgcca gatgtggaag aaactggcag
    17761 cacatttgaa gaaaatgccc gattaaaagc agaaacgatt gcacaattgc ttgatcagcc
    17821 agtcttagca gatgattcag gtttgaaagt ggatgcttta ggcggtatgc caggaatcta
    17881 ttctgcacgt tttgcagggg aacaaaaaag tgatgcgggg aataacgcca aattattata
    17941 tgaattgaca gatgtgccgg atgaaaaaag aacagcacaa ttccattgta cgttagtatt
    18001 cgcggcaccc aaaaaagaca gccttgtagt agaagcggaa tggcccggtc gagtagcccg
    18061 tataccatct ggcgaaaatg gctttgggta tgatccttta tttatcccag aaggtaagaa
    18121 acaaacagca gcagaattat cgagcgaaga aaaaaacaag atcagtcatc gggctcaagc
    18181 aatgaaaaaa ttgagtgccg agtggaaaca atggctggaa ggagagagat agatgcggta
    18241 tttagtagta agtgataatc atggagacag agatatcctc gttgacttag tagatcgtta
    18301 tcgttctgaa gtggatatct ttttccattg tggtgattcg gaattagaag cttctgaccc
    18361 attatgggat acattccaag tggttcaagg taattgtgat tatggaccag gattcgaaca
    18421 gaaaaaagtg atccaaactg gtcaggatac agtattcatg actcacggtc atttatcgaa
    18481 tgtccgattt ggtttgacac agttagcaat cgaagctcga gctgcaactg ccgatatggt
    18541 gttttttggt catacccatc aaataggctg tgagatggat cagaaaatcc tgtttttgaa
    18601 tcctggtagc atttctcagc ctagaggccc gatccaaatc ccatcatatg ctcttatcga
    18661 aagtttagat gaccaaattt ctgttcaata ctataaccgt gcccatcagg cagttgaaga
    18721 gttggccttt tctttttcga gagagcgctg aataaaaaaa ctctaatttt taataaaaat
    18781 aagctttatt ttatgaattt tgcatccggt ttcggtaaaa tagtagtgtt gaatgaaata
    18841 gaaatggagg ccatcagatg attggaccta tagtaagaga aatgctgctg gaaaatcagg
    18901 aaacgttttt gattcctgca gaaaatgtgg caaatgttat gtatcatcat ccattgtccc
    18961 atggtttact tgtgttatcg aaggtcggtt attccaaaat tcctgtatta ggaaaagatg
    19021 atcactttgt aggactagta agtttatcag atgtggtcaa taaaatgatt gacttgcaaa
    19081 caatcagcat ggacccgtta gaaggactaa cggttgctga tgtgacagaa acagatgttc
    19141 cgacaattga tgaaaattgg gaactagaag aagtactcca tttgttagtc gattcgcctt
    19201 ttttaccggt tgtcacagaa gatcggattt tcaaaggaat cattacaaga aaagagctgc
    19261 ttaaagccgt aaacttcatg gtgcatgaat tagaacgtcg taatatattg tctccgaaaa
    19321 tcgaagaatt aaaagaacaa atcgatatcg tgagctaaag aacgagaaac taaaagataa
    19381 tcaaaaaata agtagaaaaa acagcgtacc agttttgact ttatcttgaa aaagaggctg
    19441 ggacaaaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    19501 tttgtgaatt tcagcttatt tccgaaggag ttgcttctgc ttccgccgtt tatacgtgtt
    19561 tagagcgtgg gacaaaagtg ttctttactt ttgtcccacg ctctttttag tgagattcgt
    19621 tgcatcgttt gtccaaatt
//
LOCUS       DAPBCS010000052        19271 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPBCS010000052 DAPBCS010000000
VERSION     DAPBCS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.054"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(46..1365)
                     /locus_tag="RD521_002222"
     CDS             complement(46..1365)
                     /locus_tag="RD521_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7771912.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="RD521_002223"
     CDS             complement(1362..2015)
                     /locus_tag="RD521_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771913.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="RD521_002224"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="RD521_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2725..3441)
                     /locus_tag="RD521_002225"
     CDS             complement(2725..3441)
                     /locus_tag="RD521_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDT7771914.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3565..3750)
                     /locus_tag="RD521_002226"
     CDS             complement(3565..3750)
                     /locus_tag="RD521_002226"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771915.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3977..5250
                     /locus_tag="RD521_002227"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="RD521_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="RD521_002228"
     CDS             complement(5357..6388)
                     /locus_tag="RD521_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771916.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="RD521_002229"
     CDS             complement(6395..7225)
                     /locus_tag="RD521_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771917.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8037)
                     /locus_tag="RD521_002230"
     CDS             complement(7222..8037)
                     /locus_tag="RD521_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7771918.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="RD521_002231"
     CDS             complement(8034..8858)
                     /locus_tag="RD521_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDT7771919.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="RD521_002232"
     CDS             complement(8848..9948)
                     /locus_tag="RD521_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDT7771920.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..10911
                     /locus_tag="RD521_002233"
     CDS             10126..10911
                     /locus_tag="RD521_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDT7771921.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10944..11327
                     /locus_tag="RD521_002234"
     CDS             10944..11327
                     /locus_tag="RD521_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDT7771922.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11403..11534
                     /locus_tag="RD521_002235"
     CDS             11403..11534
                     /locus_tag="RD521_002235"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7771923.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11503..11739
                     /locus_tag="RD521_002236"
     CDS             11503..11739
                     /locus_tag="RD521_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7771924.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11817..12788)
                     /locus_tag="RD521_002237"
     CDS             complement(11817..12788)
                     /locus_tag="RD521_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDT7771925.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13440..15062
                     /locus_tag="RD521_002238"
     CDS             13440..15062
                     /locus_tag="RD521_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HDT7771926.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     PFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     KEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVK
                     GEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNIN
                     VYFTKARFKRLIAITVSSIVMFLWLFRMGVIP"
     gene            complement(15348..16724)
                     /locus_tag="RD521_002239"
     CDS             complement(15348..16724)
                     /locus_tag="RD521_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771927.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(16724..17380)
                     /locus_tag="RD521_002240"
     CDS             complement(16724..17380)
                     /locus_tag="RD521_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7771928.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(17390..18667)
                     /locus_tag="RD521_002241"
     CDS             complement(17390..18667)
                     /locus_tag="RD521_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7771929.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(18686..18847)
                     /locus_tag="RD521_002242"
     CDS             complement(18686..18847)
                     /locus_tag="RD521_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDT7771930.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            18917..19271
                     /locus_tag="RD521_002243"
                     /pseudo
     CDS             18917..19271
                     /locus_tag="RD521_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5973 a         3971 c         3124 g         6203 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attccttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcattttc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat gatttaaagc tggtaagtat tcatcaaaat
    10441 ggccgctttg attatatgga cgatgtggtt ttaaccaaag atcccattca aactaccaag
    10501 gaatattttg atcagatgtt gcaggtcttg acggattttc cagaaggaga tattttgact
    10561 cactttgatt atggattacg ccgcttcacc ttcacagcgg aagaattcgc ggagcatttt
    10621 gaggctcagc tgctaacgat tttcaaaaag gttgttgaac gagaattggc gatggagtta
    10681 aatgccaaga gcttcttgaa atacggcaac gaagaccttt accgttatgc cgttcctttg
    10741 tatcaaagtg tcggcggaaa gctctttacc ttaggctcgg atgctcatgt tgccgaagat
    10801 tatcagctgg gctttaaaaa aatgagtcag ctgttggcag actgtcaggt agaaaagttg
    10861 ctagtcattc aaggaaaaga acgccgcctg acgccgctgc cgattttgtg attcctatat
    10921 aatagaagaa agtggtgaaa aaaatgagca acctgtggga ctacaatcaa gaggcaccca
    10981 tccattattt aattgcgcga cattgggatg cgttgaaaat tgaggcggtt tgccgatcct
    11041 tgttagcagc cgttccaaaa caacaactgg aaaatttttt ggtggctgac agcttgcagc
    11101 gggaaaaagt tcaggcttat tttgcagcct ttaaagatca gcctttggaa tatttgcatg
    11161 cgcagtttca tcttttttat caagtagccg ccccagatga ctacaacgat ttacgaggac
    11221 agctgcaatt gacctttcaa gcggatgaaa ccgcctatac cgttttattg ggaatggctc
    11281 gccttggtga tcaggcgaag gtcgagtggc ggattttcga tatttgatgg caggatcaag
    11341 gcgtttcagt tgcaaacctt cctctctttc aattagaaaa accgcagcag gaagaagttt
    11401 gtgtgctggt caccgatgct ggtgaaattc gtctccgctt atttcctgac caagctcccc
    11461 tggctgtagc caactggcgc tccctagctg agcaaggcgt ttatgacaac acacctttcg
    11521 gctcgtgtca ttaaggaatt tgttattcaa ggtggcgctt tggatggttc tggtgatgaa
    11581 gctgtttcca gctatgaggg ctttttcgct gatgaagtgc atcgcggact ttatcatttt
    11641 aatggcgcct tggccttagg ggaccacggc ccccacacca acggcaacca gttttttatt
    11701 gtgcaaaaca caaaggcgca ggctgacctg ctaatgtaaa gcaagcatac ttggagcttg
    11761 gaggactacc ggagctggat ggtcgttata ctgtttttgg acaggtatac caagggttag
    11821 aaatcgtagc agcgatcgca gctcagccga caaacagtga agatgtaccg attgagccag
    11881 tgttcattcg acaaattaac ttcgaaaaaa catcgtaaaa agacactcac tgacttcagc
    11941 aaaagctcta ttcttgagct ggctgcagct agtgagtgtc tttcatttat ccataagata
    12001 aagcttggat tcaataaata aataaatgtt ttggttaaag ctgtgaccag caaatgagga
    12061 accgtgcgag ccagttgatc tgccaatgca aacatttctt taggtgaagg cgctggcgtg
    12121 tctgccaacg gatactccag tcccaatgct tggtaaccgt gcataccata gcagtggtac
    12181 ggtagtaatt ctaacggtac gaaccgatca agatctgcca caaaacaagc aatcgtcagc
    12241 agctcggtca ggttcatatt gatacctgga accacaggaa ctcgaattcg cacttcgcag
    12301 ttaggatact caattaaaaa gcgacgaata ttcttcaaaa tttgttggtt atctaagccg
    12361 gtccattgtt tgtggagctt gctggtaaac atttttacat ccacaaacaa tgtatccaca
    12421 tagggagcta ccttttggat ttcattataa gcaccacaaa agctggtttc cagtacggta
    12481 ttgattccaa tgtatttcga tttttgcaaa atttctttag caaaatccgc ctgaaccagt
    12541 gcctcgcctc cgctaatggt cactccgcca tccgagtgaa agtaaaaaac agcatccttc
    12601 tcaatctcat ccattacctc ttcagcggtc atggtctggc caaatccttt ttcaatctgc
    12661 ttgctttgtg attcgggaat cgaacaccag gcacagcgta atgggcagcc ctttaaataa
    12721 acaacggtcc gtaaaccagc accatcatgt agagaaccct gttctattct taatactgtt
    12781 ccttccatat agatttttga attacacact agtttaaact aaaacacaca aggaaaaact
    12841 taccatttaa tcataaaatt agctcatata aatatactag gatgtaaaat agactggtca
    12901 aaatctggtc aaaaaagagg actaatacag gtagtcctct tttttgttat tttaatattc
    12961 taaatcattt tttattggtt tattccctta ttcgatgtag actcaaatca ctatatcact
    13021 agatatcaaa cggcgtcata cggaatcaaa aacatcaaat ttgacctgtt aaatggtcat
    13081 tgaataacat tcaaaatcaa taaaacggag tcagacacgg agtcaaaaac gctgagagta
    13141 aaaaatatat acagccttgt aggtaaagta ccacagggct gtatattttt tatttcagat
    13201 taccttaaat gattccattt actttttatc tcattttttc ctactagtta gtatcaaata
    13261 ctctcctatt ttctcaataa actatttttg gatacatggt atctgaaaca gctaaaacct
    13321 tgtcattaca gcaaaaaatc acgttatcca gactgtagac aaagtccatt ttatggatga
    13381 tgtctacagt cttttctttt ataatagatt tatgaaaacg aatgcaagga gtgatagcta
    13441 tgatgtctaa aaacaatacg aatggtagaa atcaatttgc gatgcttacg attgatgatc
    13501 tagttcctca agatcatctc gttcgaaaga ttgatgccgc gcttgatttt gaatttattt
    13561 atccaatcgt cgaagccact tactccgatt taggtcgccc aagtatcgat ccagtgattc
    13621 tgatcaaatt ggtgtttatt cagtatttat tcggtatccg ttccatgcga caaaccatta
    13681 aggaagtcga taccaatgtc gcttaccgct ggtttttagg ctactccttc gaggagaaaa
    13741 tccccccttt ctcaactttt ggaaagaatt atgttcgccg atttagagaa acgacggtgt
    13801 ttgaggatat ttttgcctat attttagaac aagcagttaa agcaggtttt gtaacagagg
    13861 ataatttgta tctggattct acccacatca aagctaacgc caataaacat aagtttacga
    13921 aagaaatgac ccatgatgaa gcgaaagcct atcaggatga attagaagat gagatcaatc
    13981 gtcaacgaat agaggctgga aaacgccctt ttactttgga cttagaaaag gaagtgaagc
    14041 tgaaagaaag aaaaataagt aaagctgatc cggaaagcgg ctactacgtc aaaggcgaac
    14101 gagaaaaaca atttgcttat tctgtccata cttcatgcga cgacaatgga tttattctta
    14161 gcacaatcat cactcctaga aatattcatg acagtcaagt cgcctttcag ctcgtcaaac
    14221 aatcgaagag actttttcct gaaatcaatt gtgttgtcgc tgatgctggg tataagacac
    14281 ctaagtttgt tcactttttg actcatttga accttcgacc ttgtttgcct tattcaaggc
    14341 ccaaagggaa aaaggagcta ttatcaaaaa atgagtttct ctatgacgag tattttgatt
    14401 gttatatttg tccgcaagat caaatgctcg ctttttcaac ggttactcga gaagggtacc
    14461 gagaatacaa atccaatcca aaagaatgtg tgaactgtcc tctgctaaac caatgtacca
    14521 taagtaaaaa tcatcaacga gtcatcacga gacatgtttg gggagatctg atggatgaag
    14581 tggaacatct tcgcctaaca gatctgaata aatcaattta caaaaaacgg aaacaaacga
    14641 ttgaacgaat atttgcggat gcaaaagaaa agcatgggat gcgttggacc aagtatcgag
    14701 gcttggaaaa agtcgcgacg catacgatgc tagtgtttgc tgcaatgaat ttaaagaaat
    14761 tagccacctg gctatggaaa ggcaaggagc ctttattttt ttgttcaaaa attcgaaacg
    14821 aagtagataa aaagctgttc caagctcgag ttacgagttt ggaacagctt ttgtctacag
    14881 tctgcgatgt cggagacatc tcgttttcta ttaggaatca actcaggttc aaaagaagcc
    14941 tcccggtctc tgggaacatc aatgtttact ttaccaaagc tcgttttaag cgtcttattg
    15001 ccataaccgt ttcgtcgatt gtcatgtttc tttggctctt tagaatgggc gtcataccct
    15061 aaatggttat tcaattctcc ttgaagcatt ttttcaaata agggaccaaa cacatctttt
    15121 aaaacatctt gcatatcctc tacagattcg ggttgataag tattcaaaat ggattcagct
    15181 aacttctcag catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
    15241 attttagaaa aataaaaacc gtgaagcaac cacccgccta ggattgatgg ttacttacac
    15301 ggtttatatt acactctcat ttttggtgct aaaaagcacc gaaaatttta attattgtca
    15361 attaataatt cttttggatt ccttcttaat atattgaatg aagaaataat caatgataca
    15421 agaagtacta aactcataaa tataactaca taaatcatga atttaggtaa tacattgata
    15481 tctaaacttg ataatgtctt gttaaatcct tcagcttctg ccccaccacc taattggcta
    15541 gatgcagatt gtttagctat ttgtttagct atatcaccag taaccttatt caatatatta
    15601 ttcccaagct tgtcagctgt atattgagct aagaaatagg aaccaacgaa tgcggggatt
    15661 gatataaata ccatctcaat taggaattga ccaaaaattt ctaattttga tatacctaat
    15721 gatagtaata ctgctatttc tttctttcta gcattcatcc ataagaataa taataatgaa
    15781 actacaactc ctgcaaatat taatgagcca aaaaataatt tatttgaaat tgagtaaata
    15841 cctgatatag attgttgtaa tgcagggtaa tttgatgaac ttttaatcaa attatattct
    15901 ctccaattta tatctaattt cccaagatct tttattacac tatcaagatt cttatcacct
    15961 tttacaaaga atgttgcatc ttggtataca gctgtatctt cggtattacc ataaacttta
    16021 gcagctgtat gaatatcagt gattaatgta ttttcgtata gttcttgtgc tgcacttact
    16081 acaccgctat tatgcccatc gaatagacct ttaatttcta cttctactgt ttcatctgca
    16141 cctttctcat tatcagcatc aaataaatta gattttattt ttatcttatc tccaacttta
    16201 aggttatttt tttcagctaa atctttatgc attaagattt tatttttatc ttcattttct
    16261 aaatgctttc cttctactaa tttatatgct tctgatacaa attttgtttc ttttgttgag
    16321 tcgttaaccc cagttaacat aactgttctt ttaaaattct ttgctctttc aggtgattga
    16381 ttcgcaagag tctcttgagt ttcaataata tcataatcaa ctaaatctgc aacactgttt
    16441 attcttttta cataggagtc tatactatct gtttgagata tctttttaat atcttcacct
    16501 tttacatttc ccccacctct aggtgttcct ggatttactt gtctatttat ctccatagaa
    16561 aaactatttg ttatattagc aaatgtttct tttgaagctc tgtccgtagc atctttaata
    16621 gataaactaa tgagacttaa agttgacata gataaaataa ctaatatgat aattaatgat
    16681 tttaaacttt ttctagttac ataagcaaat gcatttttta tcattattca acctccaaat
    16741 tcattttatt tacttttttc aactttttac cacttagttc taaaatgata tccgcagaat
    16801 ctgctacttc cttactatgt gttacaacta ttacacattt atttctatct ttagctaatg
    16861 tctttaatat attaattatt tctccagcag taacactgtc tagattacca gtaggctcat
    16921 cagctagtat tattggagca tctgatacca atgctctagc aatagctact ctttgttgct
    16981 gtccaccaga taatttcata acatttcttt ttatttgttt tttatctaaa cctagttcaa
    17041 acaagatact ttcatctgct gatttattta ctaatctaat attttcaatt ggtgataaat
    17101 aatctattaa attatagttt tgaaatacta aagatatatt attttttcta tgattactat
    17161 atcctttatt ttgtatatct tcattcttaa acaatatttt tcctgtttga ggtttatcaa
    17221 gtccagcaag taaggaaaga agtgtagatt ttcctgttcc tgactttcct actatcgcat
    17281 aaaacttccc aagttcaaat ttttgattta ctcctgacaa aactttttct ttagaatttg
    17341 cataactata tgctacattc tttatttcta atatatccat tattttctcc taacttattt
    17401 ttgataagat ttctttaggc ttcttgatta acattaatga agaagcaaat acaactgata
    17461 aaacaataat acttattaat attaaataac tttgtccaag tgttgttata tttgacataa
    17521 aactactatt atttattaaa cttccaccgg aaatcattga atcctctgag ttaatgaatc
    17581 catctacaat tacttttagt aatacatttc ctaaaaataa ggaagatatt atacttggta
    17641 tcgatatgaa tattaactcg aatataaatt gcattataat ttgtatctta gatgttccaa
    17701 tagataagaa tatacctatt tcataaattc tttcccttaa ccatagaatc aagattaatg
    17761 aaagaacaac catcccacct aacataatag aataagtcat tacttttatt atatatttta
    17821 ttccactcac tgactctaaa gactcttcaa acgcattaga atctttttca acaaaatact
    17881 ttgactcatc aattttaagc tctttcaatt tgtttaaggc taagtctgta gattctgcac
    17941 taccagaata cattaaaatt ttatttgcaa tttcattatt ttctgaatta tttaatatct
    18001 cttggcttgt tgaataatct acaaacacca tattttcact aaaatcagaa gataatcctg
    18061 tatatgtttc ctgtttttta ccagaaaaga tccctataat tttaaattta tgacttttta
    18121 tttttccact tttttcaata tctagtaatt caagatcaac ttcatcacct aattttagat
    18181 tgttttgttt agcaaattct tcatgaacaa taattgaatt cttatcattt tcttctatat
    18241 ttttaccttc tttaattgta aatactccac tactaaataa aatatttctt ttagtattat
    18301 ttgtagcttc gagtgaaaca acattcttaa attcgtcaga taaatcttct ctatttattc
    18361 tttgttctcc gctaactact ttagcatctt ttagttttgc taatccatca tattgaatta
    18421 ttttttcttc aatttctttt aatttttcaa tatctttaaa ttgattaaca ttaaaatatt
    18481 taccatcttt ttttgttatt gatattgaag aattagaact ttcatataaa gccttttcta
    18541 tttcattgct tgatttcatt attgttaaac aagaatacaa gcaagaaaga actatagtta
    18601 atatgataaa aattattaaa gttctatttt tctttcttgt aatatatgct atagcatttt
    18661 ttatcacttt cctaactact cctctctatt ttcttactcc ataactaatt gcatccactg
    18721 gacaagcatt tttacatcgt ccacatctga tacactccag atgattgcaa ttctcaactg
    18781 aatctttatt catttggcaa acttttttac attttccaca attaatacac ttatccttat
    18841 cctgaatcgg ctacactaga ctagacagaa aaaataaggt gtgtagaata ataaaaaaca
    18901 cactggagga tttttcatgc caagacgtca acgaagaacc tattcaaaag aattcaaaca
    18961 acaaatcgtc gatctctatc tcgctggtaa gcctcgcgca gaaattattc gagagtatga
    19021 gcttacgcct tcttctttcg ataaatggat gaagcaagca caatcaacgg gctcattcaa
    19081 agaaagagac aacttaacac cagaacaagc agaattgatc gcactaagaa agaaaaataa
    19141 gcaactcgaa atggagaatg atattttaaa gcaagcggcg ctgatattcg gacgaaaaga
    19201 caagtaattg atgccaacaa gcataaatat tccatatcag cgatgtgcaa aattctaaat
    19261 atttctcgtc a
//
LOCUS       DAPBCS010000053        19189 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPBCS010000053 DAPBCS010000000
VERSION     DAPBCS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.055"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            238..1629
                     /locus_tag="RD521_002244"
     CDS             238..1629
                     /locus_tag="RD521_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDT7771931.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="RD521_002245"
     CDS             1688..2491
                     /locus_tag="RD521_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7771932.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="RD521_002246"
     CDS             2524..3378
                     /locus_tag="RD521_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HDT7771933.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="RD521_002247"
     CDS             3375..4076
                     /locus_tag="RD521_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDT7771934.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="RD521_002248"
     CDS             4084..5151
                     /locus_tag="RD521_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDT7771935.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..6109
                     /locus_tag="RD521_002249"
     CDS             5144..6109
                     /locus_tag="RD521_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7771936.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6154..7128
                     /locus_tag="RD521_002250"
     CDS             6154..7128
                     /locus_tag="RD521_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7771937.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7151..8686
                     /locus_tag="RD521_002251"
     CDS             7151..8686
                     /locus_tag="RD521_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDT7771938.1"
                     /translation="MKKSRLEYSLINSSISMFIYILRIVIQFVGRSFFIYFLGAKYLG
                     LNGLFTNILSFLSLAELGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIG
                     IVGVLIIPLLPLFIKNSTDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAI
                     NDFAFLFFMNIVQVIALYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVV
                     SLDKQTKDEVKKNVIGNMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQ
                     NLCKQITNSITASVGNFAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISL
                     WVGAKYMLPMSTTILIVLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSL
                     LLLIVFDMGINGVLIGTIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELII
                     SAIVVFLLNEYLANGGSTFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFRYI
                     LKK"
     gene            8743..9858
                     /locus_tag="RD521_002252"
     CDS             8743..9858
                     /locus_tag="RD521_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7771939.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            9855..11270
                     /locus_tag="RD521_002253"
     CDS             9855..11270
                     /locus_tag="RD521_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDT7771940.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            11303..12250
                     /locus_tag="RD521_002254"
     CDS             11303..12250
                     /locus_tag="RD521_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDT7771941.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(12290..13450)
                     /locus_tag="RD521_002255"
     CDS             complement(12290..13450)
                     /locus_tag="RD521_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDT7771942.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            13615..14790
                     /gene="mltG"
                     /locus_tag="RD521_002256"
     CDS             13615..14790
                     /gene="mltG"
                     /locus_tag="RD521_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDT7771943.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            14926..15405
                     /gene="greA"
                     /locus_tag="RD521_002257"
     CDS             14926..15405
                     /gene="greA"
                     /locus_tag="RD521_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDT7771944.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            15573..16304
                     /locus_tag="RD521_002258"
     CDS             15573..16304
                     /locus_tag="RD521_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDT7771945.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            16301..17368
                     /locus_tag="RD521_002259"
     CDS             16301..17368
                     /locus_tag="RD521_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7771946.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            17375..18007
                     /locus_tag="RD521_002260"
     CDS             17375..18007
                     /locus_tag="RD521_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7771947.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            18147..18812
                     /locus_tag="RD521_002261"
     CDS             18147..18812
                     /locus_tag="RD521_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDT7771948.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            18840..19133
                     /locus_tag="RD521_002262"
     CDS             18840..19133
                     /locus_tag="RD521_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDT7771949.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         6704 a         2688 c         3693 g         6104 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     5761 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     5821 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5881 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5941 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     6001 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     6061 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     6121 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     6181 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     6241 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     6301 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     6361 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     6421 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     6481 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     6541 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     6601 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     6661 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     6721 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     6781 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6841 gaaaaatgga ttattaaaaa ttatcctcaa atgtacgata aagtacatac tagagtttgt
     6901 tgggcttact ttgttgtgtt agataaatta gttttaactg aagaaaaagg actgtatcaa
     6961 gaaaaaaaac aagtaagaga gtatttaatt gataatttct tttttattat aaaaaataaa
     7021 cgattaactt ttcaaagaaa aatatctatg ttattgctta cttttggaat tgctgcgtat
     7081 aaaataccgg ttaaatatca gtataagaaa gtaaaggctg ttaattaatg ctttagttag
     7141 gatgaattag atgaaaaaaa gcaggttaga gtattcttta ataaattctt ctatatcaat
     7201 gtttatatat attttaagga tagtaataca atttgtaggt agaagttttt ttatttattt
     7261 cttaggagcg aaatatttag gattaaatgg tttatttaca aatatactta gtttcttgtc
     7321 gttggctgaa ctaggcatag gctcttctat tatttattcc ctatacaaac cattagccca
     7381 aaaggatgaa agtaaagtaa aggctttaat gaatttgtat aagaagacat atgaatttat
     7441 tggtattttt atcggcatag taggagtatt gattattccg ctgttacctc tatttattaa
     7501 aaatagcaca gacataccac atatgtatcg atattatgta ttatttttat taaattcagt
     7561 tcttagttat tttttcacgt ataaaagatc acttgcaata gcagatcaaa aaaattatct
     7621 ggttgcgatt aatgattttg cctttttatt ctttatgaac attgtacaag taattgcttt
     7681 gtacttttat tctaatttta ctctatttct aatagtgcag attttgttta ctctattttc
     7741 taatataaat atttccagaa cagttgacaa gagatattca tatttaaaag agaaagatgt
     7801 agtttctcta gataagcaaa caaaagatga agtaaagaaa aatgtcatag gtaatatgtc
     7861 ctcaaaaata ggcggggtta ttgtaatggg gacagataat atattaattt ctacatttgt
     7921 taatttaaca gctgtaggaa tttattctaa ctattcattg attgtcacaa gtgtgcaaaa
     7981 tttatgtaag caaattacta attctatcac ggcaagtgta ggaaattttg ctgtatcaaa
     8041 taaaaaaaag gattcatttg agctatttaa aaaacatttt tttatcaacc actcactaat
     8101 ctttttttct tctatattgt tattgacttt aatgaatccc ttcattagct tgtgggtagg
     8161 agccaaatac atgctaccta tgtcaacgac tattttaatt gttttaaatt atagtgtaca
     8221 ggtttataga aatacatcct tcgtttttat agagtcattt ggtttatatt ggtctcaacg
     8281 gaaaaagcct ataattgaag caggtgtaaa cttgttatta tctttgcttt tgttaatagt
     8341 ttttgatatg ggaatcaatg gagtacttat agggactatc ggatcgtctc taggatttgt
     8401 gatttggtat gaagcattta tcatatataa aacggtattt gagaaacgtt ttattcattt
     8461 ctttttaact ttctggcgaa gttttataga attaataatt tcggcaatag tcgtttttct
     8521 attaaatgaa tatttagcaa atggaggcag tacgttcctt caatttataa ctaaaacctt
     8581 aataactata gtaacaacat cgatattata tttgttatta tattttagaa gagaagaatt
     8641 tacatttatc aaaaaaattc tttttaggta tattttaaaa aaatagcttt ctataaagga
     8701 gtgttttatt tgaaaatagc tatactatct ccagctttct caatgggagg agctagtatc
     8761 gtggcctcaa aaattgcaaa aggattaata gatagtggcc atgaagtttt ttttgtttct
     8821 tacttagaaa gtgaaaaacc acaagtagaa tctaagtatt atgatttata cgaaaaaaaa
     8881 atttttttat ttaaaaattt ttctcgattt cataagatat tagagtatag attgtttgga
     8941 ggctttgttc ctagtagata tataaaaaaa gaaaaagaac aactaaaaaa agttctaaag
     9001 aaagaaaaac ctgatgtcat catattcaat acttttatac ctagtgtact attctccgaa
     9061 ttcctaaagt atgaatttcc aacagtgaaa ttaattactt ggatgcactc tgatcctgat
     9121 tacagcttaa atcatattgc taaattttat aaaaaagaat atagaaaaag ttttgaacgc
     9181 gtggattcca ttgtttgtct ttcgcaagat acaaaagaaa aactttcatt atacaataac
     9241 catattaaag ttatttataa cccattgaca ttaagcggct ctagtttaat aagtaacctt
     9301 gataaaaaaa ttatttcatt tactgccaga tacgatataa atattaaagg gtttgattat
     9361 ttatgtgaag tagctaaaaa cttacctgtt ggatgggcta taaggattgc tggagatggt
     9421 acagaagaag aacgtaaaga gctgaaaaga ttaattgaga aaaatggtgt gttagataaa
     9481 attatttgcg caggtgcatt atttggagat gaattaatca atcattatat agaaagttct
     9541 attttcatat ctacttctag aagtgaggga ctaccattag tgttgataga agcaatgact
     9601 tatggattac ctgtaataag ttttgatcat ggtggtgcta aggaaatatt acaagatgga
     9661 aattatggaa ttttaattaa gaattttgac gtacaagata tgaataatga gatagaaaaa
     9721 ttgtctaata atcgtttact tatggaaaaa tatcaaaaat tctctttaga aagagctaag
     9781 gattttaatg aaaaaaaaat aattggtgaa tggcaaacat tgttaaatga attaggaaat
     9841 gaggatgtta gaaattgagg acttcgatta aaaattttaa tttatggaaa gaatggtatc
     9901 tatatatatt ttttataggg atgattattc gtgcaataaa tttttatagt ttattaccgt
     9961 ccacttttga tagtattttt tttaaactta taggaatttc aggagttttg ctattaggga
    10021 tagatttcat aattaaattt aataaaagaa aagtaactta taacatttta ttgatagcat
    10081 atatggtgat tttagtaata acgagttttg ttcataaaga atatggtcta ggagaaaact
    10141 taaagactat aatgtgggca atcttacagt attttttgat ttatcagttt gcttttgaga
    10201 atagcgataa taaacgattt tttaaaagga taagttgggg atttatttat agttggtttg
    10261 ttttagtatc aatgtcaatg gttcttttct ttggtaaatt cggctatgag aaatattata
    10321 atgctagaca taggatcaga attggttttt tagaatcaag actgtttggg acgttttctg
    10381 atattaacaa tgctgctata gcatctttag ttgttattgt cctaatctta ttttatcttt
    10441 ataataaaaa agtgacagga ttaaataaaa tcattttttt aattaatctt ccattacaat
    10501 ttatttatat agtattaagt gggtcacgat ctacctatgt ggtaagtata ataatagttg
    10561 ccatatcatc ttttattgtt gtgttaaaac gaaatggaac aaaaaaagat ttaaaaaact
    10621 atatccttgc tatagtatct tctattggtt ctgttctgat tttattcgct ttgtttaatg
    10681 caactaagat actttttcaa gaattattac ctcatttaaa agaaataaat ttttttaatt
    10741 ttacagagcg tcataaagaa ttaatgaata atagtcaaca tattaataat actcaaacac
    10801 ctgataatac tcttgttagg aaagacgtag ctgaaaatac agatatttct aatgctagaa
    10861 ttactatatg gaaaagtgca tgggaaatat ttaaaacaag ttggcttatc ggagtctctc
    10921 cgaaaaatgt agtatcttat gcacagtcaa ttattccaga tggatatatt gcgaaagtag
    10981 gaattgccat tcataatgca tacttgaatg tattaacatc gacaggtatt ctaggaataa
    11041 ttcctttcat ggctttttta ataaagagtt ttgtgctgaa tgtttatcga atcatctata
    11101 ataaatgcat tggtaaagat tatcattttg tttactgttt agttatttta tcttatgcac
    11161 tatacgcagc tcttaacaat gaattagtct atgaaaacac tgtaggaacg tttgtttttt
    11221 ggctattttt aggaagagtt aatagcaaaa ataattctag ctatttttag taataaattt
    11281 tatcttgaaa agaggagaaa aaatgttgaa ttacttgatt accggtggcg ccggcttcat
    11341 tggctcgacg ctcgcgaatt atctaggaaa agaaaacaat gtagttgtga tcgatgattt
    11401 atcgatgggg aaaaaagaga atctggatga atcgaagcac atcacgttta ttgaagggga
    11461 tgtcgctgac aaaaatttga tggaaaaggt catgaaagaa taccatttcg attatatttt
    11521 ccatctagct gctgtagcta gtgtggcaga ttctgtagaa cgtcctctag aaactcatcg
    11581 tgtaaatttt gacagtgcgc ttttattgct tgaattggta cgaaaatatc aatctggttt
    11641 aaaacgattg gtatttagtt catcagctgc agtttatggc gatgaaccaa cgttgccaaa
    11701 aaaagaagaa tcggtcatcc gtcctttaac accatatgcc atcgataagt ttgcagcaga
    11761 acaatatgtt ttggattact gtcatttata tgatgttccg actagtgctg tccgattttt
    11821 taacgtttat ggtccaaatc aaaatcctaa ctctccatat agcggagtta tttcgattct
    11881 agtggaccga tacaaaaaac aattggcagg agaaaaaaca gaatttactt tatttggtga
    11941 tggtagccaa tctagagact tcgtttatat agaagatgtt atacaagcac ttttacttgt
    12001 tgctaaggaa gaaaaagctt taggacagca attcaatgtc ggtacaggaa aaagcactac
    12061 gctacttgaa ttgattcatt caattgatca aatcttagga acagagctag cgcttaaata
    12121 tgaagcggag cgttcagggg atattcgtga ttctttagcg gatatttcaa agatccgttc
    12181 tttaggttat caacccaaat ttgatatatt gaacggaatg gaacgttatt taaagacaga
    12241 aatcgattga aacaaaaaac cagcatcctg ctggtttttt tgtctcgact tagcttttta
    12301 aattaacacc ttccatcagt tcgtaaatct gatttcttaa atcagtggcc ggcaatttga
    12361 tgtttttatc agattggata ttactttcca tgacgattgc atcttttgtg tcttttttca
    12421 tcgccgctac tgcatggaat cgattgatat ttccctgagc aaggatataa tcatttccac
    12481 tgtaaacacc gccgctgtaa ggatgtttat caccattatt ttgccacaat gagaaaagta
    12541 gttcttttgg tataatatcg ccagacagaa cactggtaaa gaatttgttc aagtcataaa
    12601 cagacatact tatatttcct gtgcctaatt catttcggat gtctgtttca ggctcgacca
    12661 atgcttttga ataatcgtca gttgcagaca tttggtaaga tacagcatgc tgcggggaat
    12721 tttttacatt ttcataaaaa tctgtatgtt ctagatggag tggccgaatg atttcttttt
    12781 ttaaaagagt ttcataaggc tgattcatta gtttgatcaa tacccctgta agcaatgtat
    12841 aatttaatgg ttcataatga tacgtatcat aaccagtaaa tttcaaatga tgaagggcaa
    12901 actgaatgat ctcactatct gacataggtt tttttggaga agcggttcga tgaagaccag
    12961 agcgcatgta aagcatattt ttgattgaga tttgctgact tccaggaatc tgaggataaa
    13021 attcagatag ctttgtgtca aaagttatct tgccagattg aatttgtttt aggataagta
    13081 aagcagtcat cgctttttgg atcgaaccga tttgatagta aagagaagga gaattcgatt
    13141 cttttatgcg tagatttgcg tacccatatc ctttttctaa tatcactcga ttatttttgc
    13201 gtacataaat cgaaccgata taatctgatt tttgcagtaa ttgatcaatt ttgctggcaa
    13261 gcatctgctc attagatgtt aaattggaag caagttgtgc gggttccgtt ttataaattt
    13321 gtggttgagg ttgttttgtt aattgccatc ctgtaatgat gcctgttgaa aagaagctga
    13381 cagccagcaa caaaactgcg agaaaagaaa gagagatctg ctgttttttt tgatgttttc
    13441 cttttgccat attaaatcct tcctatccat ttttaactat ttttcattgt aatcctcaaa
    13501 aagagcattt gccacttaca attttgacat aatatttggt aaaataattc acggcagtag
    13561 aaatttcatg tataataaac agagttacga agatgatagt aaggaggagt tgttttgtca
    13621 ggaaacaaac aacacccaag caaagacaaa aaagaagcca gtaaggcagc tacagcagat
    13681 ataatcgaca agcggagaaa tctgcgtaaa aaagaagata agatcgtgag gaagatcatt
    13741 ttagtgatcg ctcttacatt attgattatt ggcggatttc tcgggtttac cgtatatcgt
    13801 tatgtagata gcgggttgaa gcctttagat aaatcggacg accaattagt ccaagtggaa
    13861 atcccaagcg gctcttctaa taagcagatt ggcgaaattt tggagaaaga caatattatt
    13921 aaaagtggga ttgttttcaa ttattataca aaatttaaaa acttaactgg ctttcaagct
    13981 ggttattatc agttagcgcc taatatgact cttgatgaga ttggcaagca gttgcaagaa
    14041 ggcggcacat ctgagccaac aaaagttgca gatgggaaaa ttgccattcc agaaggctac
    14101 gacatcgatc agattgctga gcgggtagcg aaggtaactg gtaaagacaa gaaagaattt
    14161 ttagatttag taaatgatga gactttcttt aacgagttgt atcaaaaatt cccagaattg
    14221 ttggaaagtg cctctaaagc tgagaacaca aaataccgtt tggaaggcta cttattccca
    14281 gctacttatg attactacac gagtacttcg cttaaggatc ttgtgattga gatggtgaac
    14341 aaaacaaata cggtcatgca aaactattat tcagctatca aacagaaaaa tttgactgta
    14401 caagaagttt taactttggc ttctctagtt gaaaaagaag gcgtaaaaga aaatgatcgt
    14461 aaaaatattg cacaagtctt ctttaaccga atcaaggcaa atatgccact tcagtcagat
    14521 atctctgttt tatatgcatt aggagaacat aaagaattgg tgacgtatga agatacagct
    14581 gtagattcac catacaattt gtataccaat accggctatg ggcctggtcc ttttgacaat
    14641 ccaagcgaag aggcaatcaa ggcagtatta gaaccagcag aaaatgacta ttattatttc
    14701 gtagcagata cgagcactgg aaatgtttat ttcgctaaaa cgtatgaaga acatatggaa
    14761 ctagtgcaaa aatatgtaaa taattcttag aaaaacaggg gctagtcatt gatcattttt
    14821 agaaaaacat ttacatttta agaaaagcgt ggtaatcttt tttggactat attcctcaaa
    14881 agattacctt ttgcggtatt ttatcgaaaa aaggagacgt tacacatggt agagaaagtt
    14941 tatccaatga ctctagaagg taaagaaaaa ttagaaaaag aattagaaga actaaaaaca
    15001 gttaaacgcg gagaaatcat tgaacgaatc aaaatcgctc gcggtttcgg agatctttcc
    15061 gaaaactccg aatacgaatc agctaaagat gaacaagcat ttgttgaagg aagaatcaca
    15121 acaattgaaa atatgatccg ttttgcacaa atcatcgata atgacggcgt agatgcagat
    15181 gaggtatcga tcggtaaaac agttacgttc atcgagttgc ctgatggaga tgaagaagag
    15241 tacacgattg tcggaagtgc tgaagcagac ccgttttctg gaaagatttc caatgattct
    15301 cctatcgcgc aagcattgat tggcaaacgt aaaaatgatg aagtaaccat tgctacacca
    15361 ggtggcgata tgttagttaa aatcgtcaaa gtagaacaag cgtagtctga ctgaaagatc
    15421 tagagagttc atcttactga tgacgcactc tagatctttt ctctttgagt attgcaggtt
    15481 tcactgaacg atcttacatc taaagtccta tagccaacag tcaaaaaaat aggtatgatg
    15541 aaatgagaat gtgtcaggag ggagaaaaag cgatgaatag ttcttggcgt ttttttgtgg
    15601 tagtcgaagc actgctgctg ttatttgctg tttggcaaat cgtaaacaat actggacttt
    15661 tgcttttagt aatattcggt attttcaata tatatctagc tatgcgcaaa tatcctcgta
    15721 ctaaatttca aaattttcaa ttgatcttag ggagcttggt catttttttt agcttagtca
    15781 acagtcctgc tttatggatg atggttgtat tggccgtttt atttatcggg ctgaaaggtg
    15841 tagagatttc aggaatagat ttgacaaaaa atgctttttg gcgaaaaaaa cagatcatga
    15901 tggtccaaac agagcaatta aaaacacata ataatgagcg taaaagacag caattatttg
    15961 gtaaccaacg tattggaaac gacgtctatg aatgggatga tatcaacatt gccatcattt
    16021 ctggtgatac tatcatcgat ttgggcaata cacttttgcc aaaagatgat aatattgtga
    16081 ttgtaagaaa agggataggc agaacacgga tcttagtacc cctgggagtg gctattcgtt
    16141 tagagcatgc gacattagta ggaaatgtct tgtttgaaga agagcagttt tccttaaaaa
    16201 acgaacagat caaaatctgc agcagtgatt atgatgaaaa tcctcgcaga ttgaagatca
    16261 ttaccaatac tttggttgga gatgtcgagg tgatacgaat atgatgggaa gaatatccaa
    16321 agcaatgcta gctgtttatt cggggattgc ggctttcctt attattctat tttcactttt
    16381 cacttatttt tatgccagta accaaagtca ttggtgggga gaattgctgc gtgcgcgttt
    16441 attatacgtt ccgcttatct tccatttgat ggcaatttca ttaggtgtag gattgatcgt
    16501 atttctattg ttatcactta ttcaaaaggc aaaatacggg aaaatcgaag aaaagcttcg
    16561 tgcactttct tctggcaatt atgaatccaa acttttgcat cagccgatcc caagcgcgtc
    16621 ggatgatctg tacatcaaag atattgatat ggaaattact aaaataaaag aaaaaatgat
    16681 cgaagtatcc agtgaattac agattatgac tagccgtcct caatatgtag atggacaaac
    16741 caaagaagag attttaaaat tagaaaggca tcgattagct cgtgagcttc atgattcagt
    16801 ttcacagcaa ttatttgcag cgatgatgat gatgtcggca ttgacggagc aagcagaaaa
    16861 aagtgagaca ccagaaatgt tccgtaagca gttgaaaatg gtggcagaga tcatcaacgc
    16921 ttcccagtct gagatgcgtg cgctgcttct tcatctgcgt ccagtcaatt tagaagaaaa
    16981 aagtttaaaa caaggcattg agcagttatt gaaggaattg cagaataaaa tccagatttc
    17041 gctaaaatgg gaggtagaag atgtaaaatt aactagttcc attgaggatc acttgttccg
    17101 aatcgttcaa gaattgttat caaacacatt aagacatgct aaggctaatg aattagaagt
    17161 ctatttgcat aaaatagata acaatctttt attacgtatc attgacgacg ggacaggatt
    17221 taatatgaat gaaaccaaaa cgggaagtta tggattgaac aatatcaaag aacgagtagc
    17281 tggtattggt ggaacagtaa aaatcattag ttttaaaggg caaggtacga gcgtggagac
    17341 caaggtccct ttgatgaagg aggcatagat aaagatgata aaagttttat tagtagatga
    17401 ccatgaaatg gtacgcttag gcgtctcttc ttatttatct atccaagaag acattgaagt
    17461 gattggagaa gctgaaaatg gacgtcaagg ctatgaaaag gcgatggcac ttcgaccgga
    17521 tgtcatttta atggatctag tcatggaaga aatggatggc atcgaatcaa caaaagcaat
    17581 cttaaaagat tggccaaaag ccaaaatcat tatcgtcaca agttttattg atgatgaaaa
    17641 agtttatcct gcaattgaag caggagcagc aggatatttg ttgaagacgt ctacagcaca
    17701 tgaaatagct gatgctataa gggctacgca acgcggcgag cgagtactag aacctgaagt
    17761 gacaacaaaa atgatggaaa aaatgagtcg gcgaaatgat ccagtattgc atgaagaatt
    17821 aacgaatcga gaaaatgaaa ttttgatgct gatttctgaa gggaaaagta atcaggaaat
    17881 cgcggatgaa ttgtttatca cactgaaaac agtcaaaacg catgtgtcga atatattagc
    17941 taagttagag gtagaagacc gaactcaagc agctatctat gcgtttaaac atggtttggt
    18001 gaaataaaaa cgatgataaa gaggataatc aaaagctgag aaaataacta ttatttttct
    18061 tggctttttt acaataatga tgcttgcttt tgtttttgga aaggctttgt tatactgaga
    18121 aaagaagata ctaaaggagg aaaatggtga aacaaaattt tgcaattgta ggccttggac
    18181 gatttggcgg aagcatctgc cgtacactga tcgaagctgg acaagaagta ctggctattg
    18241 acagtaatga agatcgagtc aatgaatata tgaatatagc cacccatgct gttgtggcaa
    18301 atgcacagga tgagatgaca cttcgttcat taggtataag aaatttcgat catgtaattg
    18361 tcgctattgg agaagatatc caagccagca tcttagttac tctgatggta aaggaaatgg
    18421 gcgtaccgaa tattttagca aaagcccaaa atgagtacca tgcccgtgta ctagagaaga
    18481 tcggagcaga tcgtgtcgta caccctgagc gagacatggg gatacggatt gcacataact
    18541 tagtatcaaa gaatattcta gactacttag agctgtcaga taaatattct ttagcagaaa
    18601 tccgagtctc caatcctaaa tttttcaaca aaacattagc tgaattaaat ttcaggcaac
    18661 gttttgactt aacagtagtc gccatacgaa gagtagatca aacggttatc gcttctcctg
    18721 cagcagatga atatgttcgt gaaaatgata atctactggt tattggagag acaggcgatg
    18781 tggatattct agacgataag atgaatcaat agaaagaaaa gagaaagtag gttgttggga
    18841 tgaaattaac ggtaacgcca aaagcacaaa agtggtttga ctcagaaatt gatctgccag
    18901 aaacttacgg aatacgtttt tttggaaaag tctacggcaa aacagaggtt catgatggtt
    18961 tttcgattgg aatgtcagtt gaacagccag aacatcctgt aaagaaagtg gaaatggatg
    19021 ggacactttt ttttatcgag gaagcagatg aatggttctt taaaggatat gatttacttg
    19081 ttgattatga tcccaacctg gaggagccaa cctatcactt taaaaaacaa taaaaaaaga
    19141 gagggtgaca caatttttgt caccttctct tttttctttt gaaaatatt
//
LOCUS       DAPBCS010000054        18314 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPBCS010000054 DAPBCS010000000
VERSION     DAPBCS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.056"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(64..264)
                     /locus_tag="RD521_002263"
     CDS             complement(64..264)
                     /locus_tag="RD521_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7771950.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(716..1036)
                     /locus_tag="RD521_002264"
     CDS             complement(716..1036)
                     /locus_tag="RD521_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771951.1"
                     /translation="MKKRVLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1081..1251)
                     /locus_tag="RD521_002265"
     CDS             complement(1081..1251)
                     /locus_tag="RD521_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771952.1"
                     /translation="MTKIISQIFVKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKN
                     IIYEQNVNNGRS"
     gene            complement(1539..2657)
                     /locus_tag="RD521_002266"
     CDS             complement(1539..2657)
                     /locus_tag="RD521_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771953.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3128..4435
                     /locus_tag="RD521_002267"
     CDS             3128..4435
                     /locus_tag="RD521_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDT7771954.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGESRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            4554..5753
                     /locus_tag="RD521_002268"
     CDS             4554..5753
                     /locus_tag="RD521_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDT7771955.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5780..7048)
                     /locus_tag="RD521_002269"
     CDS             complement(5780..7048)
                     /locus_tag="RD521_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDT7771956.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(7211..7858)
                     /locus_tag="RD521_002270"
     CDS             complement(7211..7858)
                     /locus_tag="RD521_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDT7771957.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8266..8769)
                     /locus_tag="RD521_002271"
     CDS             complement(8266..8769)
                     /locus_tag="RD521_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDT7771958.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8892..9656)
                     /locus_tag="RD521_002272"
     CDS             complement(8892..9656)
                     /locus_tag="RD521_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDT7771959.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMALTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFMVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9764..10039
                     /locus_tag="RD521_002273"
     CDS             9764..10039
                     /locus_tag="RD521_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDT7771960.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEEDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10716..11666
                     /gene="yidC"
                     /locus_tag="RD521_002274"
     CDS             10716..11666
                     /gene="yidC"
                     /locus_tag="RD521_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDT7771961.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11845..12819)
                     /gene="lacD"
                     /locus_tag="RD521_002275"
     CDS             complement(11845..12819)
                     /gene="lacD"
                     /locus_tag="RD521_002275"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDT7771962.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(12816..13274)
                     /locus_tag="RD521_002276"
     CDS             complement(12816..13274)
                     /locus_tag="RD521_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7771963.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKDVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTKIATILTKEIGEDL"
     gene            complement(13271..14737)
                     /locus_tag="RD521_002277"
     CDS             complement(13271..14737)
                     /locus_tag="RD521_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7771964.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDASMGMMVPVLAAYIAEGIAKRPGLIVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDDSKAANRTVIEKGRAGDLS"
     gene            complement(14719..15669)
                     /gene="pfkB"
                     /locus_tag="RD521_002278"
     CDS             complement(14719..15669)
                     /gene="pfkB"
                     /locus_tag="RD521_002278"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDT7771965.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            15812..16561
                     /locus_tag="RD521_002279"
     CDS             15812..16561
                     /locus_tag="RD521_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7771966.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINVLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISQKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
     gene            16827..18305
                     /locus_tag="RD521_002280"
     CDS             16827..18305
                     /locus_tag="RD521_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDT7771967.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
BASE COUNT         5635 a         3775 c         3206 g         5698 t
ORIGIN      
        1 gaaaatagac aaaaacgccc agacacgcaa atgtctgggc gtttattttt tagtataggt
       61 taattaagct ttagtaacgt ttgttgcttg aggtccgcgt tggccttctt caacatcgaa
      121 agttactgct tggccttctt ctaaagtttt gaatccgtcg ccttggatag ctgagaaatg
      181 aacgaataca tcgtttccgt tttctgcagt gatgaatcca aatccttttt ctgagttaaa
      241 ccattttact gtacctgttt ccataataaa aatcctcctt gtgtatcaac acatattttg
      301 caattacttg aatggtgtat atctggaaga tgtttatttg aaacaagcta ccacagatta
      361 ccgaacaaaa actactccta tagtatatca taccgtttac aaaaaagcta gagtttttcg
      421 acttttttta ttaaaaaaag ttttctcagc gattacataa agtgaaaaac cgaacttttt
      481 ttaaaataaa cgaaatattg ttcagttggt gtttttttgc tctgatgttc ttctttaaac
      541 taaaaaaatt attctttctg actacttatc ggttctgttt caacggtcga ggctggggaa
      601 gattctttag taggtctgat gttctcatgg tatttctgat agcctatgac agtgaccaga
      661 agaacaaggc aaaccaatac gatcaacgag attagttttt cttttttgct catatctata
      721 cctcataaat aacaggatga tgtccatcat atttctgagt atgagtaaaa gcaaaagctg
      781 cagctgtaga atgtaagaaa tgccagcaac tttcataatc acaggtcgga cagtaccaaa
      841 agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc aggaatcgca gaatcaagat
      901 ctgttgtctg tacataaaca tttttgtaca gacgtgtgcc tgtaatagga gaccgctgtg
      961 tttcttcagc aaaaactggc tgacttgtta aacttaggga tagaaacagc aaacaccata
     1021 acactctttt tttcataaag acctccttat cgataagttt ttcccaatta aaatcatatc
     1081 tcaagaacgc ccgttattca cattttgttc ataaatgatg ttttttgtat cataatcgtc
     1141 aaaaagaggt cgtgaccacg tcacaacctc ttttttaaat ggtgttccag aagtagcttc
     1201 ttcggaaata agccgaaatt tcacaaaaat ttgagaaata atttttgtca atctcttctt
     1261 atttcttgaa gctgaccact tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg
     1321 catctgcggt aataagccca ccaaacaaaa aatatgaaga gctatttttg cttgctgttc
     1381 ttattacttg atgcagaaca gctttttcac aacctctcat acacggtgtt taatcagctg
     1441 accttcggta ttagctcaaa aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct
     1501 ctaatactca ggtcaaaaca ctgttttcac aacctctttt aataacgaag aatcgctgtc
     1561 agactctttt catctggcgc atctttttga tccaaaataa acactttccc gccattaatg
     1621 atcacatcat ctgctaatgc attcaacact tgacgccgat catattctat ttcaggatct
     1681 tcaccgaaac catcagctaa gtttgacgta gcgataaata gatgcgacac ttttccttct
     1741 ttcgcagctg gaacgatatc aactaactga tcgatgaatt tcttatctaa gagtttattg
     1801 tattcttttg attcttgagc tgcgagttca cttgacagct tcttcgcccc ttcttcgata
     1861 tctctaaagg atagctgcgc aggagaagca gaaatcgcaa cgtctgcaca ataataggga
     1921 tttttagcaa tttttttgaa catcgtctga ttttctggta atgcaaataa ataaatcggc
     1981 cattgttctg gattttcgaa agaatccttc aagtacgtat caacagcttg gtaatagttg
     2041 acccaatcga tctctacttc ttcatctttt ggatttacgc catggaaagt tactccatct
     2101 cgggctccgc ctcctccatt agaagagaag ttcaaaccgc cacctgtcag ttcagatccc
     2161 aatgcagtaa cgacatcaac cggtgcccct tctggtagag atacttcttt tacttgtttg
     2221 ttgctgactt catacaattt catggagtcg cggttcaatc ccaacagata ataggaatag
     2281 ttgaactgtc catttttgat aatggccagt aaataaggaa gatcacttac atagtattga
     2341 tcgtctaccg ggatactcaa acgatggata taggtttggt cagcagttat aatgaccgct
     2401 acactcgtag ttgcactacg ccaaaacgaa ggatctccta acaaattttc aattttttct
     2461 tggaacgggg accagtcatg ttcggaatat tttttctcaa aacgtttctt tgcttctttc
     2521 gcaaaatttt tgaatttgat ttgatctttt tccacatctt gatgggcgac atgcgtattc
     2581 agcatgatcg tgacgaatgg tcctttagcg ccttctgaag tcaattgtga caataattct
     2641 ttatcctttt ttgccataaa cgtacccctc ctaaaaaaat cattcctttg tttctatcta
     2701 aagtctacca caacactatt tttccataca ataaaacgta tctaaaaaaa taaaacggtt
     2761 tctacggttt taaatttagc tattttttta agttatcttc ataatttcct caattattta
     2821 gccccagtct tcaactattc ttcatgaaat gctgataaac taaaaaaaag aataaaagag
     2881 gtggatctta tgcatttgat tatgattttc agtttattaa gtattatcag tttatttgct
     2941 ttcagcgctt cccgcttatt agatgcggaa acacagccta ttcctgtaaa agtacgtaaa
     3001 aacgaccgct aaccttccat aacgtgcgtt cttatcctaa agttttttca gaaacattga
     3061 aactctctcc cctctctgat aaaattttaa tttgtagcga aacaaattaa ttacaaggag
     3121 atcggatatg tccataaaaa aaacacgtct agtcctatta ttggctagtc tcattctttg
     3181 ccttgcttct tttctaccag ctgttgctgt gcaagctgaa gatacgttca aagtaaatgc
     3241 aaaagcagct tttgctgtag atgcgcaaag cggtaagatt ctttatgatc aagatggaga
     3301 aaaaaccatg gggatcgctt caatcacaaa aatcatcggt ctttacatcg ttcttgatca
     3361 agtaaaagaa ggaaaactat catgggatga taaagtttcg atttcagatt atgcagaaaa
     3421 tttgagtatc accccagatt tatcgaatgt tcctttgcat aaagaaaaca cctacactgt
     3481 caaagaatta ttcgactctg caatcatcca gtcagccaat gcttcaatgg tggccttggc
     3541 agaaaaaatc tctggcagcg aagctaaatt cactgaacga atgaaagaac aattgaaaga
     3601 ttggggaatc aaagatgcaa cgatcgttaa tgcatcagga ctgaataact cttatcttgg
     3661 cgaaagccga ccagaaggta ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt
     3721 ggccatcgtg gcccgtcatt tgatcttgga ttttccagaa atactggacg tatctagcac
     3781 tacgacacaa atgtttggcg aaaacacgca atcaccagtg gaaatggtca actggaactg
     3841 gatgctccca ggattcgtta attacaagga aggcgtggac ggattaaaaa ctggtacgac
     3901 tgagcttgca ggtgcttgtt tcgttggtac gatcactaaa aacgatcaac ggatcatcac
     3961 tgtcgtattg aatgcggacg gccatgcgga aaacccgagt gcccgcttca atgaaacaga
     4021 ccgtttgatg gactactgct atgacaattg gtcagaaaaa gagctcggaa aagctaacgc
     4081 aagtattcct agcctaaaaa ccattgatgt aaaagacgga aaagaatcaa cagttcctgt
     4141 tgtgcttaaa agccctgtaa aagtatgggt acgaaacgat atggatactg gcaaattaac
     4201 gatcacgcca acacttgaca agaagcaagt cactgacaac gagatccaag cacctatgga
     4261 taaaggtaca aaaatcggta ccgctacgat caccttggca gacgataaat taggctattt
     4321 agaagacaac aatgcgccat caacagagat cattacggat aaaacagtgg aaaaagcgaa
     4381 tatgttcgtg attggctggc gacgcgtcgc agaatttttc ggcaacttgt tttaattgaa
     4441 caataagaaa agcatctttc catcggaaag atgttttttt gctttttcta gtttaaataa
     4501 cagctttttt catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc
     4561 taaccagaga tctgatcttt ttagttctct atcttattgg ggtattctct atcgtttatt
     4621 ggcgaaaaca gaagaaggat ccttccttgt tcttttacgg cagtacgtgg acatttacgg
     4681 taatcttaac ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc
     4741 cattgttgtt ctttagtatc tttgcttttt cttatttcac agaaaaacga aggctgctga
     4801 atggtctttt attcaatgtt ttcctcatca gtttcggcat ctacttgttt gtcttgctgt
     4861 atgaaacgca aaatattttc cttggcggac tgatcgcctt gatcacgatt ccattgctac
     4921 ttgtcctttt atttggtatt tatggattga ttgtttttct tttttggaac ggggtcacag
     4981 ttttacgtag agaatcgcat tcattagcga atcttttaac gttgatacta gccatttttc
     5041 ttacactgtt tcttgttttt gactttttct tgttaaaata tttgccgcaa tggataaatg
     5101 ctttgttttt ctgcgttccc ctgatcttga tctatctatt tatcgtattc tacaactttt
     5161 tgactgtctc attcttgtac cagttcaacc ggcctcgata caatcaagac ttcatcgttg
     5221 tgttaggcgc tgggttgatc aatggagaaa cagtttctcc tcttctagcc aagcgaatca
     5281 ataaggcaat tgctttttat cgggctcaaa gccgtgctac attaaatccg cccatcttat
     5341 tgatgtctgg cgggcaagga gcagacgaaa aagtaccaga ggccattgcg atgaaacaat
     5401 atgccatgga acaaggaatc cctgaaagag acattcttgt cgaaaccaat tcgacaacca
     5461 ctttggagaa catgctctat tcaaaggaaa tcatggatca gcaaatgaaa gggccatacc
     5521 gcgcgatctt tagttccaac aattaccaca tttttagagc cggtctttat gcgcggcaag
     5581 ccaaattgaa agcaaatggt attggtgcaa aaacagcttt ctactacttg cctaatgcgt
     5641 ttttaagaga atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg
     5701 gattgctttt cgcaggctcc gcattcttat cgctgattac tttacttttc taaaaaaaca
     5761 aggtgtgaca aaacatatgt cacaccttgt ttttttcttt cattctctta actattggca
     5821 aaaagagtat cccttttttg accgctactt gctgagcaga ataaataccg ctgttgcctg
     5881 aacacataaa gctgatcaag cagatcataa agaagtaaac agccgcttgt gaaccgaaca
     5941 attcgatccc catcacaaag caggcaatcg gtgtatttgt cgcaccagaa aatactccga
     6001 taaagcccaa tcctgcgaga aaaggaacag aaacatggag caaaggcgct agtgctgcac
     6061 caagcgttgc accaatttca aacaatggtg tcacttctcc cccttgatat cccgcgccta
     6121 aagacaaaac tgtgaagaat aacttcccaa taaaatcata gacatgtgcc tgtccattaa
     6181 atgattcttg aagtaacgga agactcaatc cgagataacg ttgtgtttgc aaaatcaatg
     6241 ctgctgccac aactacagcc ccacctatcg tatttcgcca aacaggatga ggaatccatt
     6301 gtgcataccg tgcttttaaa aagacgatcg aacggctgaa tacccagcca atcaaaccaa
     6361 aagcaatccc agcaacaaaa actttgaaga acagcaacat actccattca gggatcacac
     6421 ccatttggta atgtaggtga gaaacaccat agctttccgt catgaaattt gcaaacagtg
     6481 cagcaaagaa gctagggaag atcgcttctg ttcggacttt cccgattgcc aacacttcta
     6541 atccaaataa tgttccagct aaaggtgtgc caaatacaga actaaaaccc gcactgattc
     6601 cactgataat caagatttcc cgttctgctt tatccaatcg gaacaaacgt gctgtattat
     6661 ctgctaacgc tccccccatt tggactgctg ttccttcacg gccaacagat cccccaaata
     6721 aatgggtagt tatggttcca aacagtgtaa gtggaataag ccgcaacggg attttttctt
     6781 ctccaccatt tccttgatcg atcactaagt tgtttccacg actagaaagt ccgccgtgat
     6841 acgcatagag ataagcaaaa agcgcaccac taactggtag tagataaagg agccaaggat
     6901 gtgatagtcg gatatctgtc acaactgtca agctatttaa gaaaaaagca gacaagctcc
     6961 ccatgaatat accgagaatc gctgtgatca ccagccactt cagcatgtac actaaaatgc
     7021 gtactggact cggtaattgt ttttccataa aaaattcctc ctcaataaaa aaacgcctac
     7081 taaagaaagg ggctatcccc agactttagt aggcgtcatt agctatcttt cgttagcatt
     7141 taaaggcgaa caccatcacc tattcacttc cattaaaaga tagcatattt ttctgtaaaa
     7201 aacaataaaa ttatcgttct ttctttacac gttcagcaaa ttcatagaaa ggagccaatt
     7261 tcgttaactg ttgattacat tggtcactat catgacaaat ataattcccc tttttcgtat
     7321 acgtgccatc agaacctgct ttcgttgtag aaagaaaaag cgaaacattc gatgttttct
     7381 gacagattgc acagattccc ttatttatcg ttggtgataa agtaccgcta atccctttta
     7441 atttttcatc ttcgtaatag accatgaatt ttttctgcgt acctggatca ttccagccga
     7501 tgtacgtatg ttcttgcaag tcgatttcat tccaagctgg ttgtttgagt tttttcactt
     7561 ttcggaataa tttctcgatt tgtggtttgc taggctgcat aaacggctga acgatttgct
     7621 tcagctcctc caatgctctt tctgcttgcg cttttgtcag ttttacgtct aaaactgtcg
     7681 ttaaaaatgt atcgatttct ggcagttcgt gatctaatat gtctcgaatc ttttcagctg
     7741 ctagtgcctg aactgtttgc accgtttttg gatcattgac agatgagtac gcatttaata
     7801 actgttctac ttctcttttt ataaaaaaat attggtatgg ttttattgtt tgttccatga
     7861 ttttaaagtc ctttcttcta accaaagtgc atactttgta gaaaaaagtg atcttctgca
     7921 aagtatgtgt tacctgaaga tcccggactc ttttaaataa gatacgtttt gcatcttatc
     7981 atcaccccct ttaatgctta ttcttctatg tacatgattc attcgataaa taaaagtaaa
     8041 ccgtatcttg aacagatagt caactgcaaa cagaaaaaag gactgtgaca aaagtcgtgt
     8101 caaagtccct tgtttcgaat gaatggtgtt caaaagcttg catctgcggt aataagccga
     8161 aacttcacaa aaatttgaga agtaattttc gtaaattcct tcttatttct tgaagctgac
     8221 cacttctgtc acaacctcaa tatggtctat tattggtttt acctgttaaa tcgaggattc
     8281 tttcccaaag taacgttgcc atctcttacg catacttgca gacatgggct gtgccgcttc
     8341 tttgactgcg cagtatttgt ccacgaatgt cacgatatat ccttctttat acttaggcgg
     8401 tgcgatcgtt gcaccccaca tgtgcttcaa aatgatatct cgctcaagat ccgacaaatc
     8461 ggtgattttt tctgcattct ttacagcaat tcttggatgg atatacgcat gtgtcccctc
     8521 gtcaaatttc gtcgtacgcc aatcatagta gaacagatca tgcaaaagtc cagcacgcgc
     8581 tgttgcttta gcattgccac cccatttttt tgcgagcaga taactatagt aagaaacact
     8641 gatggaatgc tccaaacgag tggaatgaac atgctgtgtg taattcgcta attttttcac
     8701 tgcttctgtt tctaaaagat cttgtacata agacatatat tcttcgtctt cacgccattt
     8761 ttctgtcata attatcgatc tgccaccttt ttgaatttca ttttcttctg aaattgatct
     8821 aagtatacca ccagtgttcg gattattcta gtcgctctag ataaaatagc taaagcttaa
     8881 tagttcctta atcataaaga aaaatgataa agaacgatcc ctgctgcgac tccgacattc
     8941 aatgattccg catttccttt cattgggata tatacgttct gatcagtcaa agacaagatt
     9001 tcctgactta ctccctgacc ttcgtttccc atgattatag cgaaatcctc tgttttttct
     9061 actttgtcta atgcatgtgc ttcttcattt aattctgtac cgtacaccat aaaccctttg
     9121 cctttcatta cagggatcac ttctgccaaa tctttttgta acacaggcaa atgataattg
     9181 cttccctgca tgcttcgcag cacctttgta ctataaatat ctgccgttcc tttacctaat
     9241 aaaacaccag ccataccaaa cgcatctgcc gtacggatca tcgttcccac atttccagga
     9301 tcctgtacat tatctagtaa aagccagcct cctgaaaaat ctggcgtatc ttcagatgtt
     9361 aacgccatca ctgctaagat cccttgcggt gtaggtagat ctgacatagc ggtcatgacc
     9421 tcatctgaaa ctagaaaagt agaaatctct ttgtttacag ccagccagtc tccccattct
     9481 ttcatacctc tctgtgtcac aaaaagttct tttatttctg catcagacga cgcagcttct
     9541 tcgatcagat ggaacccttc caaaagatat tctgcctttt cttctctgta tttcttttta
     9601 tgtagttttt ttaattcttt gattcttgga ttttttgctg attggatctc tttcacattc
     9661 cttcacctca cctgattata tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa
     9721 tcggtatcat tgatttataa aacaggaaag aaaggtgacg aacatgcgca aagttaaaat
     9781 gaatgtccaa ggacgtgtcc agggcgtggg atttcgctat atgaccaaga tggttgctga
     9841 tcagttaggc gtgaccggaa cggtaaaaaa cgaagaggac ggctcagtca caatcgaagc
     9901 aatcggaaat gacgacatca tccaaaaatt tatcgaagaa gtcaagaagt ctccgagccc
     9961 aagtggacga gtgcagtatg tggatgtcca agaagatccg atgattgagg aacgaaagaa
    10021 atttgatgtg gtgggataag gttgtgaaaa ggtcgcttag ttttgagtat taaggaaaat
    10081 tttgtgaaaa tgtctttcca catttttgca taaatttgac ttaataccga gaaactgact
    10141 tttgaacacc gtcgataagg ttgtttttga agcgctttgc ctcgagtatt aaggagaaac
    10201 attttcaaaa tagcttctca tattttgaaa tgtaatgact taataccgag aggcagctga
    10261 taaaacaccg tcaataaggt tgtgaaaaag ctggtctgca tcaagcatta aggactggtt
    10321 gtgaaaaata gctctccata tttttcaaca ggctggactt aatgccgtag atgcaagctt
    10381 ttgaacacca tcgataaggt tgtttttgag gcactttgcc tcaagtatta aagacaggta
    10441 gtaaaaaagc tgatctataa cttgcaatga atttgcttaa cccatatttt ccagacagaa
    10501 caaaaaagtt tttagttttt taaataagaa agaaacactt ggaatcactc ttttctttct
    10561 aagtatttct ttcttgattt tttgtctttt ttcaaaattt ttcgctacga aaaagaaaga
    10621 atatttatat gaaatttcta tgaagttgat tgaattccat gcgctttttt agtatagtta
    10681 agtttgtgta aaaattatag aagaggaagt attctatgaa taaatggaaa aactggttat
    10741 tgggatccgg acttgtcagt atcctgctct ttttatctgg ctgcgtgcgg atggattcaa
    10801 acggaaaccc tgacacttca ggaatcgttt atcgagtact cgtccatcct atgggccaag
    10861 cgattacata tctagttgag aatttcaatt ggtcttacgg ctgggcagtt attgcaatga
    10921 ctgtgatcgt tcgtatcatc atcttgccac taggtatcag tcaatcgaaa aaaacaatga
    10981 tccaatctga aaaaatgcaa gcgttaaaac cacaagttga agcagcacag caaaagctaa
    11041 aagctgcaac aacacgtgaa gaacaaatgg cagcacaagc agaaatgcag caagtatatc
    11101 gcgaaaacgg attgagtatg accggcggaa tcggctgtct gcctctattg atccaaatgc
    11161 cgatcttctc tgccttgtat tttacggctc gctacacaga aggtatccgt gaatcatctt
    11221 tttacggcat cgatctaggc tcaccaagta tggtgcttgt tgccattgca ggggtcgctt
    11281 atctgcttca aggatatatt tcaacaatcg gtattcctga agaacagaaa aagacgatgc
    11341 gtacgatgct gatcgtctct cctgctatga tcgtcttcat gtcgatcagt gcgccagccg
    11401 gtgttacact ttactgggta gtcggtggtg tgttcagctg tctgcaaaca ttcatcacaa
    11461 acgttatgat gaaaccacgt ttgaaagcac aagtggcaga agagttgaaa caaaacccgc
    11521 caaaacaagt cgtcactcca agaaaagacg tgacagaatc agcaaagcca gctaacaaca
    11581 aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac aaacaacaaa ggccgcaacg
    11641 caggaaagca gcagaagcgg aagtagatag taaataagag tgtaataaaa attcattaac
    11701 agttttagca attaaaaata tcgaccaaga tttttgaaaa agaaatctgg tcgatatttt
    11761 tatttaagtg aatgtttaat ttgaatcatc gattgatcgc tatctaattg cagcagcatc
    11821 tttttatgaa gaaacaactt tttcttatct atcaacgtag acaggtgttg ctgtttcttc
    11881 taatagtgta cttaattcct taatattttg ttttccttct tctaacatcc aactactcgc
    11941 tttttcttcg ccttcggtaa caaaaggcgc cactcccttc gcccatgttg ctcgcccaca
    12001 taagacacca ttgaatgtcg aacctgattg ttttgcaaaa cgtaatgtat cttgaaatag
    12061 cgtagcattg actcccgcac ttaaaaaaat aaacggttgt tcagtcactt tcgattgttc
    12121 atagaaatac gcggatgcct ctgcttgact ataacaagtc tcttgcccat atccttccac
    12181 aaatctcata ttcactggca cttctacttt caatacatcc acttgataac gttcttttga
    12241 aaattctcgc atggcttcaa taactttatg tggttttact tttgcatagg caagagaaga
    12301 cggatcgcca atagttgcat cataagagag gatctctaaa aagaaaggta tgccttctgc
    12361 ttcgcattct gaaccaatcc gttctataac tatttgtttt tgcacattaa tctctgctgg
    12421 ttcatcaacg tcatagtaca ataaaaattt acaagcatcc gcaccagctt cttttaacct
    12481 tttcaccgac caaatattca ataaatcagg taatcttccc ggaattttcg tatcatagcc
    12541 ggttttttca taagctagca gcaagccaca ttctttctct cgaagcgctg ctgcaggcag
    12601 gccgtattct ggatcaagta aaatagctga ggcataggga gtcaggtatt gagaaaccat
    12661 ctgtttaaat tggatgatgt cttctgatgt agcttcttta tattggttga tcattttctt
    12721 caaagcacca cgctggtcga ttgctaacgc gctaataatt ccagcttcat ccgtcaatcg
    12781 cttcaaacgt tcctgctttt ctatactcat cttcttcata gatcttcacc tatttcttta
    12841 gttaatattg tggctatttt tgtttttgat tttgcttttc tcaactgttc tgtaacctct
    12901 tttcgcatca atgatcgtgc aacgagagac aataatttca aatgagtcgt tcctgcttct
    12961 gaatctggaa taaataacgc aaaaataaaa tcaactgact gtctatctaa actgttccat
    13021 tcaattcctt tgttcagtgt gacgataatc atactaggtt cgttgattgt ttgagacttc
    13081 gcatgaggaa tagcaaaacc tgacattatt cctgtagtac cttctttttc tctttctaga
    13141 agtttttgat atacatcttt ttcgtcatga gcaattccta agcagaatgt ttgctttgct
    13201 aaaaacttaa atgcttcttc ttgactatcc aatggctgtt gtaaaaaaat gttctcttca
    13261 tgaatgattg tcatgataag tctcctgctc ttcctttttc gataactgta cgatttgctg
    13321 ctttactatc atccatcttc ttgtctttat cagttactct tttcttttgg attaatccta
    13381 gtatcactcc accaacgatc gaaccaataa gaatcgctgt aacccattgg atttttcctg
    13441 ttactacagg taatactaaa aaaccgccat gtggtgctgg aacttggacg ccaaacaaat
    13501 aagtcaagac agcagcaatt gatgagccta acatcatcgt gggtaacact cggatcgggt
    13561 ctttcgcagc aaacggaata gctccttccg taatatgagt tgatcctaaa ataaaattaa
    13621 ccaaacctgc attacgatca ttggtatcaa aatatttacc aaacaataaa gtagcaaaac
    13681 cagtaatcaa cggtggcgca atacaagctg ctgacacacc agccataaaa gtcgtgtttc
    13741 cttgtgccaa aagagctgtt cctgttacat atgccgcctt attaaccggt cctcccatat
    13801 caaacgcaca catgcagcca acaatcacgc caagaacaag tggattcgaa tgttcaaatc
    13861 cagctaaaaa gctcatcatt ccttcattta tagccttcat tggacctgcc agcaaagaca
    13921 tcacgatgcc aataaatgct acgccaataa ctggatataa gaagattgcc ttcaagccat
    13981 ctagttgttt aggtaaaatt gtcagtagct tttgcaaaaa taagataaaa taacctgcta
    14041 agaacccagc taaaatacct cctaaaaatc cagtaccgcc attcatggct acgattccac
    14101 caacaaatcc aacgattaac cctggacgtt tcgcgatccc ttctgcaata taagctgcta
    14161 aaactggaac catcattccc attgatgcat cgccaactgc ttttaccatt gccgctgtag
    14221 cattgtattg tgcatgatct ggatcaaaag aatagattcc ccaaacggca aatgaaatag
    14281 caatcaatac tccgccagct accacgaaag gcaacatatg agagacaccg ttcatcaaat
    14341 ttttatagat gctacgacca aaattagcac cactcatcgc ttctggttct aaagtatcag
    14401 cagcttcatt atctgctaag acagtacctt ttcctgccaa tgcttcgtca atcagccgtt
    14461 gtgcatcctt gattcctgta ctgacagaaa catcaatgac tcgttttccc gcaaatcttt
    14521 ctgaatggac atctttgtct gcggcaataa tgacagcatc tgcctgtttg atttcttcag
    14581 ccgttaattc attttcgatc ccaatttgtc catgggtctc tactttgatc gtgatgcctt
    14641 ttttcctagc tgcttgttct aatgcctctt gcgccatgta ggtatgcgca attcctgtcg
    14701 gacaaccggt tgctgcaatt agttgatatt ttcccatctt tcgaacctct cttccctgat
    14761 tttgatttgt ttacttaatt cctccacatc taaaaaatct gttaatcctt tgcgaaaagc
    14821 ggttgagctg cctgctgcta tgcttttacg taacgtttca tcaagcggtc gtcgattcat
    14881 gtatcctgct aaaaaagcgc ctaacatggt atcacctgca catgctgtat tgacaacttt
    14941 tccttttggc gagtttcctc gaaacaccct gtctttcgta aaaagaattg ctcctttctc
    15001 accgagggat aacaaaacat tttctgcacc taactctact agtcgttgtc cataaacaaa
    15061 gtaatcttca tctgtaacca tttttcgtcc gaaccaagaa gaaagttctt cctcgttcgg
    15121 tttcagcaaa aatggtcgat agggtaaaca atccatggct tcttgatcac tactatcaat
    15181 aatcaaatga atcccctttt cctgacaaat ccgactgatt tctattaaaa cctttggaga
    15241 cagccctctt ggtaaactcc cagaaacaca aagataatct tctgcttgta atgctcttat
    15301 ttgatttaaa aaattatgga cagctgtctg cggtatttca ggtccttgat tcactaattt
    15361 gtattcttct cccgtttgat tgacttgtgt aaatacattg atacgcgtca taccaggcac
    15421 ttcaataaac tctgtttgga tttgtttctg ctgtaaatag tcttctatat actttcctgt
    15481 gaacccagca gaaaaaccta atgccgtact tgggaaatct agcatcttta atactagtga
    15541 cacattcact ccttttccgt ttgcctgaat gtcatcctct tttgtgcggt ttaccataaa
    15601 aggatgcatc tcctctgttt caataaatag atcaattgcc aaattcatcg tgcatgtgta
    15661 gatagacata accaatgacc tccttgactc tattatgaaa tacgcgaaag agaaaatgtt
    15721 ttcttaatcg ttccaacaca aagaaaacct ttacatttat aaactcgtcg tgctattttt
    15781 taactaactt ttggtgaaag aaggaaagaa tatggatcac aaattaagtg aaacagaaca
    15841 gtacctctgg aactttatcg aacaccatat cctagagatc cctaactact ccatcgtgaa
    15901 actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga aaaagaaagg
    15961 ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca agaacatcaa
    16021 tttttctttc ttagataaag tagacaaagg tatccgccga gccattctaa aaaatgaaca
    16081 agaagtggta cgtacgatca atgtgttgga aatcggaaat attgaggatg cgatacaaag
    16141 aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa tgattggcca
    16201 agaaatgctg gtaaaacttc aattaacggg aaaaaattgt caaatgcata ctgatccaga
    16261 aattatcaaa agcatcagtc agaaactgac taagaaagac agcgttattt ttgtttcttt
    16321 gaacggcgag acaaaagaac tcgtcaccgc cgcaaagaat tgctatgatg cagagattgg
    16381 ttcgattcta gttacagcca atcattcctc tacactgaaa gactattgcg agatcaatct
    16441 cgtaggtttc aaatcagaag gttcttattt ccctgattat gaagtacggt cacgcttgcc
    16501 tctccagatt atcgcgagaa ttctgcttga cgcctatgta ttgcgtatgg gggagaattg
    16561 agaagcagtt tattatggtt ggttacagtt taaaacatca cattttttat tctttatatt
    16621 gtatagactc acgtaaactt tttatgtata aagttattca caatctataa agttacttta
    16681 agtaacaaaa aataattgaa aatacttaaa atcaaaaaat atctttttaa tcaataaaaa
    16741 aattgacagt tttattaata ataagctatt ctgttataga agaaaaaatt gtttgttatt
    16801 attagcaaca aaaaaaggag tgcatcatga aaaagttatc tcaaaaacgt ttagctcaaa
    16861 tcattattga ttctagaaaa tctctgaaaa tgacacaaca acaacttgca gtagctactg
    16921 gtatcaaccg tgccttaatt agtcgattag aatctctcga ttttatcccg tcaatacaac
    16981 aattagaatc attagcagaa gttctaaatt ttgaaccaac agatctattt ataaatgaat
    17041 caaataaatc aaacagaaca aaacatcagt cgaagaatat tgctgtagcc ggtactggtt
    17101 acgtaggtct ttccattgct actcttcttg cccagcacaa tcaggtaaca gcagtagaca
    17161 ttcttcctga aaaagtaaaa caaattaaca ataagaaatc tccaatacaa gacgattaca
    17221 ttgaaaaata tctagctgaa aaaaatctta atttaattgc aacgactgat ggtgcagccg
    17281 cctatagaga agccgatttt gttgtcattg ctgctccaac aaattatgat agtaaacaaa
    17341 attattttga tacttctgct gtagagtcag taatcaaatt ggtgttagaa gtcaatccaa
    17401 aggcaataat ggtcatcaag tcgacggtac ccgtaggcta taccgaaagt attcgtaaaa
    17461 aatacaatac taaaaatatt ctattcagcc cagaatttct cagagaatct aaggcattat
    17521 atgacaatct atatccttcg cgaattattg tcggcacgga ccaaactgac aaaactttac
    17581 gagaagcctc tcacactttt gcagatttac tccaagaagg agcaatcaaa aaagatatcg
    17641 aaactctttt catgggattc acagaagctg aagcagttaa attattcgct aatacttacc
    17701 ttgcacttcg agtatcttac ttcaatgagc tggatactta tgcagaaatg aaaggactaa
    17761 atacacaaga aatcattcac ggggtttgct tagatccaag aattggcaca cattataata
    17821 atccatcttt tggatatgga ggctattgtt taccgaaaga tacaaaacaa cttctcacta
    17881 actataacga tgttcctcag aatatgataa gtgcaattgt cgatagtaat cgtacaagaa
    17941 aagactttat agcagatcga gtgttagaac ttgctggcac gtatgaggct aacagttcgt
    18001 ggaatgcaga aaaagaaaaa gaggtagtca taggtgtata ccgtcttaca atgaagagta
    18061 attcagacaa cttccgtcag tcatctattc aaggagttat gaagcgaatt aaagcaaagg
    18121 gggcaactat tgtaatttat gaaccaacac ttgaaaataa cactacattt tttggttcaa
    18181 aagtaataaa taatttagat gattttaaaa atatcagtca cagtattatt gctaatcgtt
    18241 acgatagtaa tcttgatgat gtaaaagaaa aagtatatac cagagattta tttcaacgag
    18301 attaaaaaat aaca
//
LOCUS       DAPBCS010000055        18124 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPBCS010000055 DAPBCS010000000
VERSION     DAPBCS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.057"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..724)
                     /locus_tag="RD521_002281"
     CDS             complement(26..724)
                     /locus_tag="RD521_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDT7771968.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(717..2069)
                     /locus_tag="RD521_002282"
     CDS             complement(717..2069)
                     /locus_tag="RD521_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDT7771969.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2292..3692)
                     /locus_tag="RD521_002283"
     CDS             complement(2292..3692)
                     /locus_tag="RD521_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDT7771970.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVNMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3853..4590
                     /locus_tag="RD521_002284"
     CDS             3853..4590
                     /locus_tag="RD521_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDT7771971.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4715..5194
                     /locus_tag="RD521_002285"
     CDS             4715..5194
                     /locus_tag="RD521_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDT7771972.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5191..5958
                     /locus_tag="RD521_002286"
     CDS             5191..5958
                     /locus_tag="RD521_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771973.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6188..6757
                     /gene="lepB"
                     /locus_tag="RD521_002287"
     CDS             6188..6757
                     /gene="lepB"
                     /locus_tag="RD521_002287"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7771974.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6861..7463)
                     /locus_tag="RD521_002288"
     CDS             complement(6861..7463)
                     /locus_tag="RD521_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDT7771975.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7569..8038
                     /locus_tag="RD521_002289"
                     /pseudo
     CDS             7569..8038
                     /locus_tag="RD521_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8144..8728
                     /locus_tag="RD521_002290"
     CDS             8144..8728
                     /locus_tag="RD521_002290"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDT7771976.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8731..9804
                     /gene="prfA"
                     /locus_tag="RD521_002291"
     CDS             8731..9804
                     /gene="prfA"
                     /locus_tag="RD521_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDT7771977.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9797..10636
                     /gene="prmC"
                     /locus_tag="RD521_002292"
     CDS             9797..10636
                     /gene="prmC"
                     /locus_tag="RD521_002292"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDT7771978.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10629..10808
                     /locus_tag="RD521_002293"
     CDS             10629..10808
                     /locus_tag="RD521_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771979.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10830..11855
                     /locus_tag="RD521_002294"
     CDS             10830..11855
                     /locus_tag="RD521_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDT7771980.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12030..13274
                     /locus_tag="RD521_002295"
     CDS             12030..13274
                     /locus_tag="RD521_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDT7771981.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13427..14374
                     /locus_tag="RD521_002296"
     CDS             13427..14374
                     /locus_tag="RD521_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7771982.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14508..15137
                     /gene="upp"
                     /locus_tag="RD521_002297"
     CDS             14508..15137
                     /gene="upp"
                     /locus_tag="RD521_002297"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDT7771983.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15276..15518)
                     /locus_tag="RD521_002298"
     CDS             complement(15276..15518)
                     /locus_tag="RD521_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7771984.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15639..15711
                     /locus_tag="RD521_002299"
     tRNA            15639..15711
                     /locus_tag="RD521_002299"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15672..15674,aa:Thr,seq:cgt)
     gene            complement(15830..16468)
                     /locus_tag="RD521_002300"
     CDS             complement(15830..16468)
                     /locus_tag="RD521_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDT7771985.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16591..17223)
                     /locus_tag="RD521_002301"
     CDS             complement(16591..17223)
                     /locus_tag="RD521_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDT7771986.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17574..17873
                     /locus_tag="RD521_002302"
     CDS             17574..17873
                     /locus_tag="RD521_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7771987.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6214 a         3161 c         3571 g         5178 t
ORIGIN      
        1 tgtcacattc ccctttttcg aataactagg ctgtttgtcc ttcaagatag ggcagatcca
       61 attctttacc gtaacgttgt tctaaagcta ggcggatcaa acgagctgcc aggttctcat
      121 ttcttgcaag gataggtcca tggaaataag aaccgaagac atttcggtaa atgacacctt
      181 cagattggtc ttcaccatta tttccttgac cttgtaccac tttccccaaa ggtcgttcac
      241 cttcacctaa gaaagtccga ccattgtggt tttcaaatcc ataataagtt tcattgaatt
      301 cttcgttatg gataacgata tcaccgataa accggttatt ttcctgactt aacgtgtagt
      361 gatccagtgc actgattcct tggatctttt ctccattagc tcccatgtag tagtgaccta
      421 gcaattggta gccaccacaa atggctaaca tgacgccatc attttcgata tagttgatca
      481 aagaatcttt ctttttctgg atgtcttttg aaatgattaa ttgctcaaag tcttgaccac
      541 cgccaaagaa aaccaagtca tatttatcag catcaaaagg ttcatgtata ctaacgatct
      601 ccgtatggca agaaaccccc attttttctg ctatatattt caacatcaaa aggtttccgt
      661 tatcaccata tgtgttcatc aaatttccat aaaggtgggc gatattgagt tcataattag
      721 ccacggttca ttcctccttt gataaatcct tgagcagcta gttctttacg aagctgcaat
      781 acagcagtgt aagtagctaa aatatagaca tgctcagttg gcaattcttt gattttttcg
      841 attacttctg gtaattcttt tgtttctgtc aatttatttt ctggaatccc ggcaactttt
      901 agacgtaaag tcatatctgt atgacggtct cctccagaaa taacagctgg gatatccata
      961 tcggcaaagt tttcaaaatt accatcccaa atccagctga catcgattcc gtctgcgtaa
     1021 ttggcattta ataaagccac taaagaaaat ggataaggag ctagaccaat catatcgatt
     1081 acttgattga tacctacagg atttttgaca agtactaatg tacatttttt cccattgata
     1141 ttgatgactt cttgtcggcc gaatactttc tcgtcatatc ctaaaccttg tttgatttta
     1201 tcaggatcga cattgaagta ctctgccact gcagtagctg ctaaagcatt gtagacatta
     1261 tacataccgc caacttcgat tccgtattca tgaccatcga tcacgaaatc tgctgacaca
     1321 ttgtccatcc gtaccatatc agttaatcga taatccaatt caggacgttt gaagtcacaa
     1381 tttggacagt aatatttccc taagtttgca taagtgatca ttttataatg caagatgtga
     1441 tgacaatttg gacataagac accgtctgta ttgtaatgag ccatttgttc acgatcttct
     1501 tcatggtcaa agccataata ttttctagga ttgactgttt cgatagaatt gaagattggt
     1561 gaatcaccgt tactgataat cgttgcattt ggtgactttg cggcaccgtc tacaatcatt
     1621 ttataggtcg tatagatttc tccataccga tccatttgat cacggaaaat atttgtaaat
     1681 aagaacagtt ctggtttcat atattctgtg actttgctca aacttgcttc atctatttct
     1741 aggacagcaa actttttctg tccttttttt ggttttgcct gcaagaaagt cgatacaatt
     1801 ccctgtacca tattcgcacc agtagggttg gttagaactt catcgaattg ctggcgcaaa
     1861 atattgactg ttaaagcggt agtcaatgtt ttaccgtttg ttccggtgac aacgacgact
     1921 tggtagtctt ttgccaaact atctagtata tgtggatcga ctttcagcgc tagtttacct
     1981 ggtaaacttg atccgccttt aaaaaaagtt tttaatgccc attgcgacgt ttttccaact
     2041 gcaatcgcaa aatgactgcg tattcccatt atgtaacctc caaaaacgat ttgctgacga
     2101 ataaattcaa ctaatcatac catatgttga aaaaaaggtt aaattgcttt tcttattctt
     2161 aagaaaaggt tacggctgaa agtgcatgaa atcggacaaa atcgcatgat ttaacaaaaa
     2221 aacagcttga aacaaaatga ttgtttcaag ctgctgtgat tttattattc cctcttacct
     2281 atattcccat tttaaccgat aataattttc tcgcttggat attcatagcc tagatctcgt
     2341 gtttcttttg gcacgaataa catcaatgta tatagcattc cgatccgccc aataaacatc
     2401 agtgtagcaa tcgtgatttt gcccacaaca gacaagtctc cggtaatccc taaagataaa
     2461 ccagttgttc caaacgcaga agttacttcg atgatgatcg agatcagcgt ttgttcttct
     2521 gtagcagata aaaaaacgat acaaaagaaa cacataccaa gagaaagcat aaagacaacg
     2581 actgattttc gcacatcatc ttgatcgatt ctccgtccaa aaatattgat gttatcttca
     2641 cttttgagga aggaatatag atacaaaccg atgattgcta cagtagttgt tcgaatcccg
     2701 ccgcctacag aactaggact gcatccgata aacatcagta aagaaaaaat gatcagtgtt
     2761 gtgatttgaa aatcacccaa atcgtgaatc tgcagaccag cattacgagt cgtaatcgaa
     2821 taaaacatcg aattgatcca tttgacactc atattggaat cttggaataa atggtccttt
     2881 tccaatagat aaatcaagac agttcccgaa acaaacaaga taacaaaagc taacacagct
     2941 aatttggtaa acaaggaaaa acgaaacgga agcttcgcat tggaacgttt gtataatagc
     3001 cattcccggc actccatcaa tacaggaaaa ccaattcctc cgacaaaaat caaaaacatg
     3061 atcaggatca agaaaaaata gtcatgtgca aagggcttga tggaatcccc agtgacgtca
     3121 aacccagagt tcgtgacagc cgatatcgcc tgataaaaac cgtaaaaaac ggcatcacgc
     3181 caacgatcaa aatagcctct gtaataaaaa taaatagaaa agaatgtccc aaaaagtaat
     3241 tgaaaccaga ttaaaatagc aaaagtgatc cgaatcagtc ggacgatacc agacaaacgc
     3301 ggttgattca tatccgtcat gatcagctgt cgctgtttca gtgtgatccg tcgtttagaa
     3361 aaaatgacaa aagcagtaga aatcatcatg atccccaaac caccaacttg aaataatatt
     3421 tccagcagta taataccgtt atcattgaac acagaattga tatcaaaagt cgatagtcca
     3481 gtcacactca ctgtactgat tgccatgaac agcatgttca caaaagaaac atggctatct
     3541 ggttcgcgaa aaatcggcat ataaaataga acgagtgata agaccgtcat caatatataa
     3601 taaaaaacga tgatttggat cgatgagaaa tgattggcag caaacttctg tcctcgccgc
     3661 tgcagccttc gaaagaataa atcaacatgc acagtactat cctcctgaca atagtcgttt
     3721 ctaccagtat acaaggggaa ttaggaatac tcaaagaaat tatcaaacag ataacaatac
     3781 agctttcgat taaaatagaa atggcttata ataaggaaaa tcctagaaaa aagaaggagg
     3841 aggaataaca aattgattaa tgctcaacaa atcgtagaaa aaatgaaaaa tcaaaaaata
     3901 aattatgata cagttctgaa aaaaatgatc atgcaatggg agaaagaaga agtaaagcct
     3961 aagatattaa tacatacatg ctgtgctcct tgcagtacgc acacattgga attcatgtct
     4021 caatatgcag agatcacgtt atttttttca aattcgaata ttcatcctaa aagtgagtat
     4081 ttgcgaagat tacatgaaca aaaacgattt gtagaccaat ttaatgagaa aactgaccat
     4141 catgttgcgc taatcgttga tgaataccgt ccaaatgaat tcaccaaaat ggttttaaca
     4201 aaccatcttg aaaacgaaaa agaaggtggt gctcgctgca gtgcatgctt caatatgcga
     4261 ttagatcttg ttgcgcaaaa agcgcaagaa ctaggatacg attactttgg aagtgccctg
     4321 accatctcgc caaagaaaaa tgcagctttg atcaatcaga tcggaatgga tatccaaaag
     4381 atttacggga cacaatactt gccaagtgat ttcaagaaaa acaaaggcta tgaacgctct
     4441 ttgcaaatgt gcaaagagta tgatatttat cgccaatgtt attgtggttg cgtgttcgct
     4501 gccaagcagc aaggatgcga tctgaaagaa atcaatcggg aagcaaaagc ttacttaaaa
     4561 acatcaaccg tagtttttga ggaaaattaa agaaatcttt cggaaaaacg taagaaaaaa
     4621 ataaagaatt tatggtagaa ataaggtaga ctgtataatt tgtccgtagt aaagtaagga
     4681 aacaagaata aatttcaatg aaggaagaga taagatgcga aaagtagcac ctattttgaa
     4741 gccaatcgct gtattaacaa tctcgacgat tgtcgcgact actacaggta ttgtgaatag
     4801 ttccagctac cgtgcaccaa gttcaagttc tagtactgta gaagaaacaa caccaactac
     4861 ttcagtctcg acatctgaac caacaacaac ggaaagtttg aatgaaacca ctacgacaag
     4921 tacatgggac gatacaacaa ctagttcgtc gtatgatgag acatatacaa ctgacagctc
     4981 aagcgaaaca agtaatacgg taccagatgg cactccatcg gattcaacca atccaagtac
     5041 aagtccggat acaagtgcta gctccgaaac gataccaagc agtacgactg tagatgataa
     5101 cacgcaaaat gatgatgcag cacgtacagc acagcaaaac caagattcag gaaataccca
     5161 aacgtataac cagcaagcag gtgattctca atgacatttt tactagacct tatcaacggg
     5221 gtgcataaat tccttggcta tttagacatc agtcctaaat atcttaaccg tggatatacg
     5281 attttaagtg tgattcccac gctttattta ttgagaattg tttatggact ttggcaaaac
     5341 caaaactatc ttcaattttt cttatatgga cttgcatttc tagtgcttgt ttattttact
     5401 gttttaaatg tattttatta ctttttagat aaaaacacga aagccgatgt cactcagctt
     5461 tttgtgaagt atttacctga tgaagcgttc aacatccaga cagaagaatc gatagataat
     5521 ggaaattcga tcgataaagt gaatacacaa gaggtcatgg tttcttatga cgaagattat
     5581 caattgaaat tagccgagaa tatgcggtat ttgattggaa atggtgagat caaaacaaat
     5641 gaactcggtc gcatggatgg atttctggtc gatagaaata cattgtatcc ttattactat
     5701 gtcaagaaaa caggagagaa agagtatgtt ctaaatattg gacgaagcta ctcggacatg
     5761 gaaaaagtag ggaccattcg acttgactca acagaagaca cattggaacc tgtcggattg
     5821 ttcatcgtcg gcggtgattt tgtcaaagac gggttacgct atcatgagcc ttatcgtctg
     5881 aaattgattg caaaaaaggc acagtcgcag gaagaaccag tggtttatag tcgtagccaa
     5941 agaagaagac attcataatg tgatgaaacg ccgtgtatca gtggttgcga taaaaacgtt
     6001 tattcggaat aagggggatg tgacacgtct tttgtcacgt tcctttttta tataacgaga
     6061 aagataagac gaacacatca cttattcttt cctttcattt ataaacattg tagaataaag
     6121 aaagtacaac gaagtattgt gaaaaaacgt aataaataac gaggaaaata caagtaggag
     6181 gtaagctgtg aaagaatttt tgaaaaattg ggggattttg atccttgtat tagcggctat
     6241 tttattagct agagtgtacg tgtttacacc agttactgta aacgggcatt cgatggaccc
     6301 tacattaagt gatggacaac gattgatttc atctaagatc tcaaactatg aacgaatgga
     6361 tattattact acaaaggaac cgggggatga agaacggatg attgttaaac ggatcatcgg
     6421 tatgcctgga gatacagtca agatggaaaa tgaccagttg accattaatg gcaaaaagta
     6481 tgatgaacct tatctggatg agttcaaaaa agaattttca gaagataagt tacaaggaga
     6541 atatgcttat agctcaggat tccaagcaca agcagaaagt tcatccacct ttacgagtga
     6601 ctttgaatat actgtgccaa aaggaaagta tcttgttcta ggcgataacc gcttgatttc
     6661 aaaagacagc cgaatgtttg ggttagtcga caaagatatg atccaaggaa aagttgtttt
     6721 ccgttattgg cctctttcag aaattaaaat catgtgatga aaaaataaaa agaagctgtg
     6781 acatgttttt tgtcacagct tctttttccg aaaattcctt cttatttctt ggagttgctc
     6841 actttttttg caatctcatt ttaagctttt tggctgatgg ataagaaaag cgaagcgttt
     6901 ttgttcataa atagcttttc cccacgatgg aaggtatgac tagtagcaga gacaaagccc
     6961 gttttttcca atcgattatt caattcttct tggctaaagc cattatggac tttcggatga
     7021 ctaatttgtt cattcttgtc aaagtctacg atgatcaatt gaccgccagg agctaaaatt
     7081 tcataaagtt ttgtaagaat attctccgta tcaggaatgt gtaaaagtac aagtgataga
     7141 aggatgagat tagcttggac agcaggaaat tcgacagaag catcagcgtg gatcgttcgg
     7201 acatttttca gatctgctgc ttggatcttt tcttctgcca ttttcagcat agtttctgaa
     7261 gcatcggcaa tgatcagttc tttgaatctt tccgcaagcg gtaaactaac aagcccagtt
     7321 ccgcctccgt aatccagcag gactttttgg tggctgtcgc gcggtagata agtagttagt
     7381 tcttcgcgaa taatcttggc taatgcttga cggtcagggg aatcataatg gttagcgatg
     7441 gaatcaaaaa tattttttgt catcaaatag ctccttacgt aaaactttga aattttaact
     7501 tattatagca cgtgaaaaga aagaaagcat gctatactca attcaaaaag ggagaatcta
     7561 gaaagaatat gccgatatcg tttgtaaaag atagagagga aaaaggaaag tgtgtccgag
     7621 aaatattact agacttgcct gagtggtttg ggcttcctga atcaacggaa aaatatatcg
     7681 aggaatcaag taaactacct ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta
     7741 tcactctttc acagacaagt gaagatactg ccgaaatcta ttctattgta tgggaataaa
     7801 aaaagactat catcaccaag gattaggaac aaaacttttt aaagaagcag aaggttacgc
     7861 agcaaaacat tacaaatatt tgcaagtgaa gactgtagac gaggggcact actctatata
     7921 tgatcaaact atttgtttct acgaatcatt aggattttca aggttggaag tttttccaaa
     7981 tttatgggat gaatggaacc catgtttagt tcttgtcaaa aaattggaac aaaagtgagc
     8041 aatagattta atactaatct gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat
     8101 caagtataat accataggaa caaactggaa ggtgaaaaag agcatggcac aactattttt
     8161 taagtatggc gcaatgaata gcggcaagac gatcgagatc ctaaaagtcg cacataatta
     8221 tgaagaacaa aataagccag ttgtcttaat gacaagcggt attgatacaa gagatggttt
     8281 aggcgttgtt tcaagccgaa tcggactgaa acgtaatgcg atgccgatat ttgaagaaac
     8341 agacgtattt gacgtaattc agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga
     8401 agcgcaattc ttaaaaaaga aacatgtcat tgcttttacg agaattgtgg acgaattgga
     8461 tattccggtt atggcatttg gtttgaaaaa tgattttcga aacgagttat ttgaaggatc
     8521 aaaatattta ttgctttatg cagacaaaat cgaagaaatg aaaacaatct gttggttctg
     8581 ccacaaaaaa gcaatcatga atttacatta tattgatgga aaccctgttt atgaaggcga
     8641 tcaagtgcaa attggcggaa atgaagctta ttaccccgtt tgtcgaaaac attactttca
     8701 cccatcaatg attacagaag gagaataagc atgtacgatc aattacaagc aattgaagac
     8761 cgttatgaag agcttggaga acttttaagt gacccagaag tgatttctga tacaaagcgt
     8821 tttatgcagc tttcaaaaga agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt
     8881 tataaagaag tagtacaagg aatcaccgat acagaagaac tgcttggtga aaaactggat
     8941 gatgaaatgg ctgaactagc aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc
     9001 ttagaagaaa aaatcaagat cttgctttta ccaaaagatc caaatgacga taaaaatatc
     9061 atcatggaaa tccgtggagc agcaggcgga gacgaagcag cattattcgc tggcgatctt
     9121 ttcaatatgt accaaaagta tgctgaagca caaggctgga aaacagaagt gatggaagct
     9181 aacgttaccg gaatcggtgg atataaagaa gtgatcatga tgatcaccgg agacaacgta
     9241 tattcgaaat taaaatatga aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa
     9301 tcgcaagggc gtatccatac ttccaccgca acagttgtcg tgatgccaga agctgaagaa
     9361 gtagagatcg atattgaaga caaagatatc cgtacagata tctatcatgc ttcaggtgct
     9421 ggcggacagc acgtcaataa aacagcatct gctgtacgtt tgactcactt accaacaggg
     9481 atcgttgttg ctatgcaaga tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa
     9541 atcttgcgtg ctcgtgtgta cgataagatc cagcaagaag cacaaagcga gtatgatgcc
     9601 aatcgaaaat cagcagtcgg aactggggat cgttcagaac ggatccggac gtataatttc
     9661 ccgcaaaatc gtgtgactga tcatcgtatc ggattaacta tccaaaaatt agatcagatt
     9721 ttagcaggga aattagatga gatcgttgat gcattgatca tgtatgatca aacatcaaaa
     9781 ctggaagcta tgcaaaatgg gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc
     9841 ttttttagag gatcatggga aagaagcata tagtatccaa tttttatttt tggaacgaaa
     9901 acaatggaaa aaacttgatt ggctccttca tatgaacgaa gagatttcag aagaagaaca
     9961 gcggttgatc gaaaccgatc tgcgacttct gcttgcagat catccacctc agtatttgct
    10021 aggatatgca gatttttatg atcatcggtt aaaggtaact gaagcaacac tgatccctcg
    10081 accagaaaca gaggagttag tcgaatggtg tttagacgag acgcctggtg taccgttaga
    10141 agtgatagat attgggacag ggactggagc aattgctatt agtttgaaag cagcaagaaa
    10201 aaattggcat atatctgctg tggatctttc agaagaagca ttagaagtag cgaaagaaaa
    10261 tgctcagaaa gaaggaacga aaatcagttt ttatcatgga gatacattag gaccagtaat
    10321 ggatcagcaa tttgatgtga ttatttctaa tccgccgtac atcagtcgga atgaatggga
    10381 actgatggat gaaagtgtcc ggtcattcga acctaaaatg gccctttttg ctgagaacga
    10441 tggattagct atttatgaaa aaatagcagt tgaagcacca agcgtactaa aatcaaatgg
    10501 aaagattttt ttagaaatcg gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca
    10561 agcatttcca gataaaaaag tggcgataaa aaaggatctt tttggcaatg aacgaatgat
    10621 ccaagtttat ggttgaagtt gtggatgaaa aattttttat ttgttttttt attataaaaa
    10681 aacacttatt tgacaaggat gtaaacgact ttttggttta tcaacaagtt tatccactat
    10741 tgacaaatag attatccaca aacagtggaa aacttgagga taaactcaga caaaataaga
    10801 atgtatgaaa aaggggttgt ggataaactg tggaaacgaa acgatatgat gaaacagaat
    10861 tgcaagaggc agccgaagca ctgaaagaag gagaacttgt tgcttttcct accgaaacag
    10921 tatacggatt gggcgccaac gctcttttac caaacgctgt caagaaagtg ttttctgtaa
    10981 aagggagacc gcaagataat ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag
    11041 agtatgtgga caactttcat ccggcagcag agaagattgt gaagaacttc tggcctggac
    11101 cattaacttt gatatttaag atcaaaaaag acactttgcc ctcagttgtg acgggaggat
    11161 tatccacagc tgcatttcgt ataccggata acaaaaaaac actagaagta atcgaacttt
    11221 ccggtgttcc gcttgtcggt ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg
    11281 cagatcatgt atatcatgat ctacaaggaa aaattaccgg gattattgat gatggagcta
    11341 cgcgaattgg tgtagaatcc actgttcttg atttaagtga tccaacagcc atgcctatga
    11401 ttttacgacc aggtgcagta acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg
    11461 ctattgatca gcatcttgta aaagaaaatg aaacaccaaa agcccctggg atgaaataca
    11521 aacactactc acccgataca cgtgttctga tggtcagaga tggcgattgg tcaactgcag
    11581 ttcaatgggc aaaaaataag aagatccgtg caggtgtcat agcaagcccc gaaattgcgg
    11641 atcaagtgcg gacagatact gcagctgttt atatgtataa cgacaactca gtagaagcgg
    11701 cggctaaagg attgtttgca ggtctaagag gattagatga accaacactt ggattagatt
    11761 tgatttttgt acaagtgtat ccagaaactg gattggggaa tgcttacatg aatcgcttga
    11821 aaaaagcagc aggacaaaat tattttgaaa aatgatcaat tggttattgt cgcattcttt
    11881 tagtatgata caatgctatc aagtaaatct tctatcagtg aacgaaaaac taagcgtaac
    11941 taatcgtaat agaagagata cgaatatttg gcaatttgtt tttgatggga aaaataaaat
    12001 tgtaggaaaa taaaaaggag ctgttgtttg tggtagatta caaaacgttt gaccctgatc
    12061 tttgggctgc gatcgccaaa gaagaagaaa gacaagaaca taatttggag ctgattgcct
    12121 ctgaaaactt tgtctccgaa gcagtgatgg ctgcacaagg aagtatcttg acaaataaat
    12181 acgcagaagg ttatccaggg catcgctatt atggtgggtg tgaatttgtc gatatcgttg
    12241 aaaatttagc gattgaccgt gcaaaagaat tattcggagc gaaatttgcg aacgtccagc
    12301 ctcactccgg ctctcaagcc aacacggctg cttatctcgc tttggtagaa ccaggggaca
    12361 cgatcttagg aatggatctt tccgcaggcg gacatctaac acatggatca ccagtcaact
    12421 tcagtggcaa aacctatcac tttgttgctt acggtgttga tccgacaaca gaagttattg
    12481 attataatgt ggtacgtatt ttagctagaa aacatcaacc aaaattgatc gtagcaggtg
    12541 ccagtgctta cggacgaacg atcgactttg cgaaattcag agaaatcgca gatgaagtag
    12601 gcgcaaaact gatggtagat atggcacata tcgcaggatt agtagcagca ggacttcatc
    12661 ctaatccagt tccttatgca gatatcacaa cgactaccac tcataaaaca ttgcgtggcc
    12721 ctcgaggcgg catgattttg acaaacgatg aagcattagc aaaaaaaata aatagtgcgg
    12781 tatttcctgg tattcaaggc ggccctctag agcatgtgat tgcgggtaaa gcagtagcat
    12841 tcaaagaagc attagatcct gcatttaaag aatatagtga acaaatcatc gccaatgcaa
    12901 aagcaatggt caaagttttc aatcaagcaa ttggaaccag agtcatttca ggggcgacag
    12961 ataatcatct gatgttgatc gatgttcgcg agttaggtat aaatggaaaa gaagcagaga
    13021 gcattttgga cagtgtgaat atcactgtaa ataaaaactc gattcctttt gaaacactaa
    13081 gtccattcaa aacaagcggt attcgaatcg gcacgccagc aatcactact cgcgggttca
    13141 aagaagaaga tgctgtaaaa gtggctgaac tagttgtcaa agcattacaa gcaaaagatg
    13201 ataacgctca actagacgaa gtcaaaacag gtgtacgcga attaactgaa aaatttccgt
    13261 tgcataaaaa ataaaaattt aaattccatt agtaaacaga aaagttcaat gttgggcttt
    13321 tctgtttatt ttttgggaaa acggattctt tgacaatatc ggtgatgctc tctagtatat
    13381 gaggtgtaat tttagtcttc acactaaatc tcagggagga aaagtcatga ctgatcgtat
    13441 ctcgttagaa aaggaagcag tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta
    13501 tgaactagca ccggaagaag gaagagtttt actagaagaa gtacaagaat cacctgtgaa
    13561 caaatattcc gtcgatattg aagacttgat ggtggatact ggcgaatggg gaaaactcaa
    13621 tattcgtctc gtacgaccag aagggaatat ggataaatta ccagttattt tttatattca
    13681 tggagcaggc tgggtttttg gcagtgctca aacccatgat aaattgatca gagaacttgc
    13741 ggttcgtacg aattccgtcg ttgtctttcc agaatatagt cgatcaccag aagcaaaata
    13801 tccaacagca attgaacaaa attatgctgt gttgcaacag ctgaataaat tagccaagtc
    13861 aaaaaatctg gatctcgaga atctaatcgt tgcaggagat tcagtaggtg gtaacatggc
    13921 tacagtcatg acgatcttaa caaaagaacg aaagggcctc ccaatatcca agcaattgct
    13981 gtattatcca gtcacaaacg ctgattttga tacagattcc tatcatgaat tcggagaaaa
    14041 ctattttttg acaagagaag gaatgatgtg gttttgggac caatatacga cgaatgaaga
    14101 agagcgtgct gaaatcacag cttcgccttt gagagcaaat ataaatgaac taaatgatct
    14161 tccttcagca atgattttaa cgggagaagc ggatgtatta agagatgaag gtgaggctta
    14221 tgcgcgcaaa cttcgtgatg caggggttcc tgttacacaa gtacgcttcc aaggaatcat
    14281 ccatgatttt gtcatggtaa atgctatgga tcagacaaag gcaacgcgtg cagcaatggc
    14341 attatctact tcttggatca agaaaaaaga ataagctttg cttgtccgca ggaaaacttc
    14401 ttttcaaaaa cggattttct taggaaaaat ggcgagtttg actagtcaat cacttgattt
    14461 tgttttacaa ttaaaaagaa acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc
    14521 aagtgatcga tcatccattg attcaacaca aactaactat tattcgggac aaaaactgcg
    14581 gaacaaaggt ttttcgtgaa gtagtcgatg aaatcgctat gttgatggca tatgaagtat
    14641 cacgggatat gccattggaa gacatcgtga tcgaaacacc gatcactgaa acaacacaaa
    14701 aaacgttgtc tggtaaaaaa gtagcgatta ttccaatctt gcgcgcaggt attggaatgg
    14761 ttgacggtat cttggaattg attccagctg ctaaagtggg acatatcggt ctttatcgag
    14821 atgaagaaac attggaacca catgaatatt tcgtcaaact tccagaagac attgatgcac
    14881 gtcagttgtt tgtagtagat ccaatgttgg caacaggcgg atcagcaatc atggcaatcg
    14941 atgcattgaa agaacgtggc gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg
    15001 agggagtaaa agctttgcaa gaagcgcatc ctgatattga catctataca gcttctctag
    15061 atgaaaaatt gaatgaacat ggttatatcg ttcctggact aggggatgcg ggtgaccgtt
    15121 tgtttggtac aaaataagta aatacgaaaa agggtgtgac acaacttttg tcacatcctt
    15181 ttttggtcat ttttaacgtt tgatgcagaa cagctttttc acagctttcg tttgcatata
    15241 aaaaaaccat cccaaaaatg cagagatggt caaattcaaa tcttttttag ttctttgtag
    15301 ttaacgatta ctacaccctt actttgcgtc atcaagttcg cattttctgc agtttcatca
    15361 atctcaacaa cagcggaatt tttcaattgc tttgaaatcg ttccagtttg cgacttgccg
    15421 tgaattgtga acattacgtg agtattgatc ttgaattttt caccaatttc cgatggtacg
    15481 atagttgtat caaattttcc aaaagttgcc attttcataa aacctgcctt tcacatcaat
    15541 tataccacgt tttatgaaaa atatcatgaa attaaactaa ttttccatcg gactctttac
    15601 aatcagccaa atagagtata taatttcttt tggataaggc cagcttagct cagttggtag
    15661 agcaacgcac tcgtaacgcg taggtcgtag gttcgatccc tatagctggc attaaaagag
    15721 aagacagata tatcaaggat ttttaatcca ttgatacatc tgtctttttg tttaaaggaa
    15781 ataaagtatg ctttcttagc aatagagcat gtattatttc tataaaaatt taggagaata
    15841 aaacgactag caatatagct gtgaaatcaa atatcacatg aactgcccaa cttgtaatga
    15901 tcgagttgct tttaatggca gcttggttga ataacaatct tgccacccct tgtatcaaaa
    15961 ggatgtgtgc tacagtgtgt aaaatatttc cattatcata agtactgtaa tggataaatc
    16021 caaagatgat tgctgacaag aaactagcga caggaatgct gaacttacta gaaaaaatag
    16081 cgagaaaata cactgagaac aattcttctc caagtatcat gataggcaaa ataataaata
    16141 ataacgggct gaaagcctca tttattggat tgcctgttaa accccatttc aaaatttgtt
    16201 gcaaaaatac cgagactgcg gcactgatta ctacattgat aaaaaaatat ttggctatat
    16261 ttttgacagg agcgacaggt ttcgagaaca tttttttcgt atctttttta ccaaaaacaa
    16321 gtgtaaatgc tataaatcca gtgatagtta aagaaaaaat gctaataatg gatggcgcaa
    16381 atagcaaccc taacaaaccg agtacaacga caatagtgcc caatatccct tttttaagtt
    16441 ctattttttt tcctaaaatg ttattcatga taataactcc tactattcag tttttgaaga
    16501 tagagtcagt atacatgaag agttaaaata ggcaatgttt ttttatggat atttgtattg
    16561 ctaaaaattt atcttatata ttcattttga ttatactact tttatttgta aaaattttct
    16621 agcaaaacca agtgcttgca actgattaag cgtacaattt tttagatgtt ccgagcgtac
    16681 ttcaatcatt tcaaaggagt tgctacttaa atcgatattt tttaaatcag tatcaaaaaa
    16741 gctagcattt tctaaatcat tagtagaaaa cattatttta gtgagggaac agtctacaaa
    16801 atctgcgctt gataagacag agtgctcaaa ggtactttct tttatgtttg aaccagataa
    16861 atttacatag tccattttac aattctcgaa taacgtgttc ccaatataac tttccatcaa
    16921 tttagtacct gttaatttac aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa
    16981 tatacagcct gaaaaatcac agttttcgaa tatacagtcg gtaatctcca ttctttgaaa
    17041 atcattggaa gcaaaaaatg catacttgaa aatagatttg taaataattg gatggcagat
    17101 tatttggttt tcgatgacct ctttttgtga aaggatatta tacagttcaa aatcttcgtt
    17161 ttcatagata tgaaaaaagg atcctttatt caagcttgct tcattaactt taggaaaaat
    17221 catatttaat tacctccatt ttcataaagt catattaaca aaagctaaac caacttacat
    17281 tagcagaaaa tgagataggt tttcgccttt ttcgataacg atagtgtcgc tatctcttga
    17341 aacgttctta gaattgccct gagagacaca aattaggcgt aattctaata aaaatcttag
    17401 aataaaaatt tttactgatt taatttagaa tgtttttaaa ataaaaatcg ttgccataac
    17461 agtgtattga aaggttttaa caatccagta atgatttaaa aaaatctaga tgtagcgtat
    17521 actttgttta tagaatgtag tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta
    17581 aaagtgaaga aatagaaaac atggtactaa aattatatga tctcgtttta aatccagaga
    17641 tcaaagaaaa ggaacgtata cttttgatag acgcaaagac aggcttagaa aaaggtcagt
    17701 attatcctaa agtcataaat aatttggaac gtagtttacg tccgttagca atcaggggag
    17761 aattgtctaa accagtatca ccgttttata tggagatatc aacaatagga aaatttgaaa
    17821 aagaattagg tcgaggaatg gcttcggcac cgattacttt tgggcattta taaaaacgag
    17881 ttattcatct caatgaatta aaaagtatag tcaatctcat aatgcaggct aggttcattt
    17941 atttgaacag agtctgtatt tttttattct aaataaagaa acagatgttt ctttatattg
    18001 tattttatcc tttaattaac acaaaaacag ctagtataaa ctaataatgt taattaaaag
    18061 tgtattttat gaaactatcg ttatttatta atgttgcatt tttgtaagtt aaaaattgga
    18121 aata
//
LOCUS       DAPBCS010000056        18107 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPBCS010000056 DAPBCS010000000
VERSION     DAPBCS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.058"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(164..388)
                     /locus_tag="RD521_002303"
     CDS             complement(164..388)
                     /locus_tag="RD521_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDT7771988.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="RD521_002304"
     CDS             complement(586..2166)
                     /locus_tag="RD521_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDT7771989.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="RD521_002305"
     CDS             complement(2567..3943)
                     /locus_tag="RD521_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDT7771990.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="RD521_002306"
     CDS             complement(4071..5189)
                     /locus_tag="RD521_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDT7771991.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="RD521_002307"
     CDS             5331..5765
                     /locus_tag="RD521_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7771992.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="RD521_002308"
     CDS             complement(5843..6376)
                     /locus_tag="RD521_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDT7771993.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="RD521_002309"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="RD521_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDT7771994.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="RD521_002310"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="RD521_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDT7771995.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="RD521_002311"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="RD521_002311"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDT7771996.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="RD521_002312"
     CDS             complement(10101..10679)
                     /locus_tag="RD521_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDT7771997.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="RD521_002313"
     CDS             11001..11471
                     /locus_tag="RD521_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDT7771998.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="RD521_002314"
     CDS             complement(11579..12805)
                     /locus_tag="RD521_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7771999.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="RD521_002315"
     CDS             complement(13559..13759)
                     /locus_tag="RD521_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7772000.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="RD521_002316"
     CDS             complement(13893..14501)
                     /locus_tag="RD521_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDT7772001.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="RD521_002317"
     CDS             complement(14586..15056)
                     /locus_tag="RD521_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDT7772002.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="RD521_002318"
     CDS             complement(15104..16018)
                     /locus_tag="RD521_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDT7772003.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="RD521_002319"
     CDS             16164..16967
                     /locus_tag="RD521_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDT7772004.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="RD521_002320"
     CDS             17145..18092
                     /locus_tag="RD521_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDT7772005.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5438 a         3631 c         2964 g         6074 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaata
//
LOCUS       DAPBCS010000057        17806 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPBCS010000057 DAPBCS010000000
VERSION     DAPBCS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.059"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(59..187)
                     /locus_tag="RD521_002321"
     CDS             complement(59..187)
                     /locus_tag="RD521_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDT7772006.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="RD521_002322"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="RD521_002322"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDT7772007.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="RD521_002323"
     CDS             complement(1674..2729)
                     /locus_tag="RD521_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDT7772008.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="RD521_002324"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="RD521_002324"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDT7772009.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="RD521_002325"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="RD521_002325"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDT7772010.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="RD521_002326"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="RD521_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDT7772011.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="RD521_002327"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="RD521_002327"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDT7772012.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="RD521_002328"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="RD521_002328"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDT7772013.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="RD521_002329"
     CDS             10491..12050
                     /locus_tag="RD521_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDT7772014.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="RD521_002330"
     CDS             complement(12161..14092)
                     /locus_tag="RD521_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7772015.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="RD521_002331"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="RD521_002331"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDT7772016.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="RD521_002332"
     CDS             complement(15021..15773)
                     /locus_tag="RD521_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7772017.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="RD521_002333"
     CDS             complement(15932..16141)
                     /locus_tag="RD521_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772018.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="RD521_002334"
     CDS             complement(16152..17480)
                     /locus_tag="RD521_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772019.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="RD521_002335"
     CDS             17616..17771
                     /locus_tag="RD521_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772020.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         4723 a         3834 c         3217 g         6032 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttt
//
LOCUS       DAPBCS010000058        17540 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPBCS010000058 DAPBCS010000000
VERSION     DAPBCS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.060"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(39..848)
                     /locus_tag="RD521_002336"
     CDS             complement(39..848)
                     /locus_tag="RD521_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7772021.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1141..2577
                     /locus_tag="RD521_002337"
     CDS             1141..2577
                     /locus_tag="RD521_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDT7772022.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2747..3943
                     /locus_tag="RD521_002338"
     CDS             2747..3943
                     /locus_tag="RD521_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDT7772023.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4005..4190)
                     /locus_tag="RD521_002339"
     CDS             complement(4005..4190)
                     /locus_tag="RD521_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDT7772024.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4421..7735
                     /gene="dnaE"
                     /locus_tag="RD521_002340"
     CDS             4421..7735
                     /gene="dnaE"
                     /locus_tag="RD521_002340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDT7772025.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            7903..8865
                     /gene="pfkA"
                     /locus_tag="RD521_002341"
     CDS             7903..8865
                     /gene="pfkA"
                     /locus_tag="RD521_002341"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDT7772026.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8939..10723
                     /gene="pyk"
                     /locus_tag="RD521_002342"
     CDS             8939..10723
                     /gene="pyk"
                     /locus_tag="RD521_002342"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDT7772027.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10758..11660)
                     /locus_tag="RD521_002343"
     CDS             complement(10758..11660)
                     /locus_tag="RD521_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7772028.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11846..12415
                     /locus_tag="RD521_002344"
     CDS             11846..12415
                     /locus_tag="RD521_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDT7772029.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12473..12652
                     /gene="rpmF"
                     /locus_tag="RD521_002345"
     CDS             12473..12652
                     /gene="rpmF"
                     /locus_tag="RD521_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDT7772030.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12822..14243
                     /gene="gndA"
                     /locus_tag="RD521_002346"
     CDS             12822..14243
                     /gene="gndA"
                     /locus_tag="RD521_002346"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDT7772031.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14436..15122
                     /locus_tag="RD521_002347"
     CDS             14436..15122
                     /locus_tag="RD521_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7772032.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15119..16624
                     /locus_tag="RD521_002348"
     CDS             15119..16624
                     /locus_tag="RD521_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7772033.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            17311..>17540
                     /locus_tag="RD521_002349"
     rRNA            17311..>17540
                     /locus_tag="RD521_002349"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5675 a         3057 c         3679 g         5129 t
ORIGIN      
        1 attgtcacaa cctctttttt gtggaatcta attctctatt atagtttaag tagtttttcc
       61 agataaacag ctaaaccgtc ttcctgattg ctcaaaggag tgacatcatt tgcaatgcct
      121 ttgagttggt cagtaccgtt agccatagct actccccaac ctgcataatc caacaattcc
      181 aaatcattat gctcgtcacc aaaggcaatg acgtcttttt gatcgataga caggtagtta
      241 gcaatctctt gcacgccttt tgctttttgg attccttttg aaacaatctc taagatggca
      301 gtaggtccac cccatgtccg aacatcgaca taactgccaa attgacgtgt taattcagca
      361 gatacagctc ctgcaaaacg tttgtctgtt cggacaagca aagatgtagg atttgtcgtc
      421 aaatttttag gagaaagcaa attcttctct cctggacgag aagaagcaaa aattttttca
      481 tcaaagaaat caaaactgtc aataaaaaaa gtatcacgat tttcagcagc gataaaatct
      541 aaatttagct gctttttttg cgataagatt tcaaaagcga tttcacgatt gatcaacgtt
      601 tctctttcac catcccagtg ctgattaggg atgtgtacta atgcgccgtt aaaattaacc
      661 atcggtgttg ttaattctaa ttgttggtaa aactgatggc tcattcggaa tgggcgtcct
      721 gtagcaatac tgacatgatg accggcattg attgcttttt ttagagtctc ttttgtttta
      781 gatgtaatca tcgaatcttg attgagggta gttccgtcca gatcgatggc aattaatttt
      841 ttattcaata agggtccttc cttcctcaac tagcatttgt tttagtctag catattttag
      901 gtaattaaca agagcaatca gctattccta acaaaaacta cagttatggc tatataaata
      961 gctaaacata agctaaacac gaacgttaag tattattttc gctttttttg ttaaaaaaac
     1021 gttgaaaagc gaatgaaacg tctatataat ttgaaattgt tcgatggatt tgccctattt
     1081 tacttatttt ttattattat agttttccat cagaaaaaaa ttcagggagt ggaacttttt
     1141 atggaaaagt ttttcggttt aaagaaaaac aatacaaatg tatcaactga aataatggcg
     1201 ggagtaacga cattctttgc catgagttat atcttattcg taaatccgac gattttgtca
     1261 gcatcaggta tgcccttcca agcagtattt ctagcgacaa tcattgcatc gattattggt
     1321 acgctggtaa tgggattgtt cgcaaatgta ccatatgctc aggcacctgg tatgggattg
     1381 aacgcatttt tcacttttac ggtcgttttt gggcttggtt attcttggca acaagccttg
     1441 gcaatggtgt ttatttgcgg attaatcaat atttttatta ctgtcacaaa tattcgaaaa
     1501 atgattattc gggcgattcc cgaaagtctt caacatgcga ttggtggcgg gatcggtatt
     1561 ttcgttgcgt atgtcggaat caaaaatgca ggattcttat ctttttcagc agaccagtca
     1621 gctatttcga gctctgtcgt tgaaggcgga aaagcgacaa atgttacgat aaatggtgga
     1681 atcgtccctg cgttagccaa ctttgacaac gctcctatct tattggctgt gattggtttg
     1741 gtactgacag ccattttagt tgtaaaaaat gtccgtgggg ctattcttat cggtatcgta
     1801 gcgaccacag tccttggtat ttttatgggt gttgttgatt taagttccat tgattggcaa
     1861 acgaattcgt taggtaattc atttaaagaa ttaggaacaa ctttcggtgc agcatttggt
     1921 tctgaaggca tgcaatcttt attcagtgat tcttccaaga ttcctcaagt attgatgacg
     1981 atcatcgcat tcagtttatc tgatactttc gatacgatcg gaacatttat cggtacagga
     2041 cgccgcacag gtattttctc aaaagaagac gagatcgcgt tggaagatgg tcgtgggttc
     2101 aaaacaaaaa tggataaagc actatttgcc gatgcgatcg ctacttctgt gggggctatc
     2161 tttggtacat cgaatacaac aacttatgtc gaatctgcag ctgggatcgg ggccggtgga
     2221 cgtactggtt tgacttcagt agtagtggca gtgctattcg cattaagtag tttattctct
     2281 ccactgatcg ccattgtacc aaatcaagca actgcgccag cattgatttt agttggagta
     2341 atgatgatgg cttcttttgc agacatcaaa tggctagata tggaagaagc tttaccggcc
     2401 ttctttgcat cgatctttat gggattgtgc tatagcattt cctatgggat tgcagcagga
     2461 tttattttct atacaattgt gaaagtaatc aaaggaaaat acaatcaagt ttctgttgct
     2521 ttgtggatcg ttgatatttt attcattatc aacttcatca ttctagcaac aatttaacta
     2581 aagagaaggg attggaacag tatttgttct gatctctttt tgttttacat aaaaagaacg
     2641 ttacatataa aatgaccttt gcgggatttt tctcttttta ctggcgagat cgttgcttgt
     2701 ttattcgaaa ataggtatga taaaagagac gaaagaggga tgatagatgg agattaattg
     2761 gagaaaaaat ttagtgattg cgtggattgg ctgttttttc actggtgcaa gtattagttt
     2821 agttatgcca ttcataccag tttatgttga gcaactagga acaccaaaag atcaaatcga
     2881 attgttttcg ggactagcaa tctctgtgac tgcttttgca tctgccgtgg ttgcaccaat
     2941 ctggggaaat ctagctgatc gaaaaggccg aaaactcatg atgatccgtg cagcagcagg
     3001 gatgacatta acaatgggat ctttggcttt tgtaccaaac gcgtattggc ttttgatcat
     3061 gcggttttgg aatgggattt tgtctggata tattccaaat gccacagcga tgattgcttc
     3121 acaggcacct agagaaaaga gcggatgggc gttaggaaca ttgtctaccg gagcgattgc
     3181 tggaaatctg atcggacctt caatgggcgg tgcactggct cagtggtttg gtatggaaaa
     3241 tgtattcttg atcacagggg ctttgttgat gatcacaacg gtattgacca ttttcttagt
     3301 caaagaagat ttccatccaa tcgagaaaaa agatctgata agcaccaaag aaattttttc
     3361 aaaaatggat catctctcta ttttgatcgg tctcttcatt acgaccttga ttttacagat
     3421 aggaatcact acgatcagcc ctattttgac cttatatatt cgtgaattga gcggaagtac
     3481 agaaaatatc ctttttgtca gcggcttgat tgtttcgata gctggtgttt ccgctgtctt
     3541 ttcttcaccg aaactaggaa aactagggga taagattggg aatcaaaaag ttctaattgg
     3601 aggactcatt ttatcatttt gctgttattt acctatggca ttcgtcacta caccgctcca
     3661 attaggtata cttcgcttta ttttaggctt ttcaactggc gctttgatgc cttctgtcaa
     3721 tacactgatc agtaagatta cgcctcaaga aggagtcagc cgaatctata gctacaatca
     3781 gatgtttagc aactttggac aagttttagg tcccatgcta ggatcgacag tcgcgcatgc
     3841 ttataattac agtgctgttt ttattgtgac aagtttgttt gttctgtcaa atattcttct
     3901 ttctttattc aactttcgta aagttcttcg cagcagatta taatggtggt gaggagtaga
     3961 aaataaaaaa gagcttgaga aatctcaagc tcttggctgt ttttttacga atgggcagag
     4021 gtttcttttc ttggcgcaac tgcaccaatc aaaaggataa tgacacccgc aatgaatgaa
     4081 atgattgttg tcaaagtaaa atcatatgtt ccgcttgtta atgcagcacc aatataaccg
     4141 actacttgac ctagaacgac agcccagaat aatgtaacga tatatcgcat aaagaacacc
     4201 tcttttctat cttaatttta ccagattttc tttgaatgta aagaaacgag accaaatctc
     4261 aataaaattt gagttcttgc gaaagaactc attcctacag attacaagca agcaaatgaa
     4321 gtggaataat caacttcagc gcctgctatc ttaatgaaag taaaaggatc aaacctttta
     4381 acgctagtga attttaacga aaaaaaaggc ggaatgcaag atgtttttcc cacaacttta
     4441 tacaattact tcttattctc ttttacaaag tacgatacgt attcgagaat atgtacaaga
     4501 agcaaaaaaa caaggctaca cttcattagc cataacggat aaagatgtct tatacggtgc
     4561 acctgaattc tatcgtattt gtgtagaaga aggagtacag ccaatcattg gattacatct
     4621 tagttatact tatgaagaaa aaaattatgg cttgcttgcg tacgcaaaag atgaaatagg
     4681 ttatcagcag ctgatgaaac tttccagccg aaaaatgatc caagaaaaat tggaacttgc
     4741 agaaatgaca gaaaccgacc atctagcgtt tgttgttccg gcaaatcatt ctgtttctga
     4801 ggctttcaat aaagaggata actttttcgg tcattggcag tttttacttg aaaatgtttt
     4861 tactagaaat ttttatatcg gcttgacaga aaacacgcct gatcaattgc ctgaatggta
     4921 tcagtatgtg ataaaagaaa attattcgct ttgtgcatta catcccatcg ctagtttaca
     4981 acaagaagaa ctttttgcac ttgaagtgat gaaacatatc aaagagggaa gccaaatacc
     5041 tttagaaaat ttatctgcat ctttaccact aacagcaaat gtctatcttc tttcagaaca
     5101 agaaatgatc aaacgattcg aaaaagcggg tctttcccaa gctgtaatag gcgcccagcg
     5161 tttagctgaa tcgatttcat ttgaattcca gttccatcag aaattacttc cgcattatcc
     5221 agttcctaat ggaaaacaag ctggagaata tttaagagag ttatgctatt cgcaagtgga
     5281 acgccgaatc actgactata caaaggaata ccaagaacgt ttggactacg agttatctgt
     5341 catccatcaa atgggattcg atgattattt cttgatcgta tgggatgtaa tggcttttgc
     5401 tcatagaaac aaaatcgtta ccggtgcagg tcgtggttct gctgcgggtt cattagtagc
     5461 ctatgtgtta gaaatcacag atgtggatcc attagaatat gatttgctgt tcgaacgctt
     5521 tttaaacccc gaacgcttta cgatgccaga tatcgatttg gatattcctg acaatcgacg
     5581 tgaagaagta ttgaattatg ttcgagaaaa gtatggacaa tatcatatgg cacaaatcgc
     5641 aacatttggc acaatggcag caaaaatggt tcttagagat gcagctagag tgtttggttt
     5701 atcgcaaagt gaagcaaacc gctggtcaaa agcaataccc aatcaattga aaatcacatt
     5761 gacccaagct tataaaactt cgcatcaatt agtagaatta gttcaggcaa gcgaaaaaaa
     5821 ccaactttta ttcaaaactg cagtgatttt agaaggattg ccaagacatg tttcgactca
     5881 cgcagcaggt gtagttatca gcgataagaa tctacttgaa ctagttccgc tccaacgcgg
     5941 ttctgatgaa gcttttctga cccagtatac gatgaatgag gtagaagcaa tcggtctttt
     6001 gaaaatggac tttttaggac tacgcaactt atcgattatt gattttactt tgaaagctgt
     6061 taagcgagta gaagggtatg agatccgatt aaaagagatc ccgctaaatg atgcaaaaac
     6121 gctgctcttg tttcaacgag gcgaaacctc aggagtattt caatttgaat ctgctggtat
     6181 ccggaacgtt ttacgaagat tagatccaga aaacatcgaa gatattgccg cagtcaatgc
     6241 attgtatcga ccaggcccta tgcaaaatat agataccttc attgccagga agaaaggaaa
     6301 agaagccatt cgctacccag atgattcact gatcccgatt ttgaaaaaca catatggagt
     6361 gatcgtctat caggaacaaa tcatgcagat cgcctcgact atggctggat ttacattagg
     6421 gcagtcagat attctgcgac gggcagtgag taaaaagaaa aaagacgtat tagatgaaga
     6481 aaagtcgcat tttgtatcag gagcattaca acagggatat tcggaaaaaa cagcagcgac
     6541 cgtatatgaa tatatcgaaa aatttgcaaa ctatggattc aatcggtcac atgcatttgc
     6601 ttattcattc gtgggattcc aaatggcgta tctaaaagcc catcatccag gtgctttctt
     6661 taccagcctg atgaattctg ttcggcataa cacatcaaaa ttgaaagaat atattgcaga
     6721 agcacgtaaa aataaactca aactttcctc gccgtccatt aatcaaagta attccagttt
     6781 tgagttggtc aataaggaag aaatcagatt cggactgaca gcgataaaag gtattcggcg
     6841 tgattttgta gaagatatat taaacgaaag aaaacaaaat ggcccgttcc agtcagtgga
     6901 tcaattcctc atacggatcg ataagcgttg gctgaaatta gagttactgc aatcactgac
     6961 agcagtagga gtttttgatg aattatctcc taacaggaaa caattgatgc ttgatctcga
     7021 aggaaaaatc caaaatatcg tttacagtgg tggaagtctt gacttgctgg gaatcatggc
     7081 attaaaagaa gaagaagtcg ctgactatac actagaagaa cgcttgaagc tggaagagga
     7141 gtacttaggt atttatttgt caggtcatcc tgcagaaggg tttgatcgtc ttcgtttagc
     7201 taaacaagtt cagctgatta cagatatcgt ccctaatcaa ccagtcaatt tattagtgat
     7261 gatcaaggaa gttagagaaa ttcggacaaa aaaaggagaa ctgatggcct ttgttgaagg
     7321 aaccgacagt agtggagaaa tttctgtcac agtttttcca actctttatc gtaaatatcg
     7381 taatttgttt gccgttgatc gagtgatctt tgttcaaggg cggagcgaaa tcagcaaata
     7441 taatcaggaa ctgcaactaa ttggagaaag ctttgctgat cctaaccagt tagaagccca
     7501 gtatccttca caaacttgct atttgcgtat tacagaagaa cgggaagatc ccgagataat
     7561 gaaacaatta caagtgattt ttcagaaaca cccaggatat ataccagtag ttatgtatca
     7621 cgaaaaaacg caacgaaaag tagttctgtc agatgcttat tgggtggatg acagcaagag
     7681 tataaagcgg caacttattt atctgcttgg cgaagataat gtggttttca aataacttac
     7741 ggtaaaaaat actacttgct ttcatttttt ttcatttttt tatccgtaaa tcaagacaag
     7801 tgagaaaaat aatgttagaa tgttagtcgt ggttaacgca aaaatggttg taaacctaag
     7861 tcatacttag gataaagtat tttaatgagg tgaatgttta ttatgaaacg catcggaatt
     7921 ttgaccagtg gcggagacgc acctggaatg aatgcagctg ttcgtgccgt tgttcgtaaa
     7981 ggtatttacg aaggactgga agtatacggg atcaattatg gttttgccgg tttagttgct
     8041 ggagatattc gtcgtcttga tgtggcagat gtaggtgaca agatccaacg tggtggtaca
     8101 ttcttgtatt ctgcacgtta tccagaattt gcaactgaag aaggtcaatt aaaaggaatc
     8161 gaacaattga aaaaattcgg aatcgaaggt ctagtggtta tcggaggaga cggctcttat
     8221 catggcgcaa tggctttgac aaaacgcggt ttcccagcag taggtattcc tggaacaatc
     8281 gataatgata ttcctgggac tgatttcaca atcggttttg atacagcaat caatacagta
     8341 ctagaatcaa ttgaccgtat tcgtgacaca gcgacttctc atgttcgtac attcgttatc
     8401 gaagtaatgg gacgcaatgc aggagacatc gctttatggt caggtgtggc tggcggagcg
     8461 gatgaaatca tcatcccaga acatgatttt gatatggctt cagttgctaa aaaaatccaa
     8521 gaaggccgcg atcgcgggaa gaaacattgc ttgatcatct tggctgaagg tgtaatgggc
     8581 ggaaatgaat tcgctgagaa actttcagaa tttggtgact atcatacacg tgtatctatc
     8641 ttgggacacg ttgtccgtgg tggagcacca agcgcacgcg accgcgtgtt ggcaagtaaa
     8701 ttcggtgctt acgctgtcga attgcttcaa gaaggaaaag gtggattatg tgtcggtatc
     8761 catgacaacg aaatcgttgc gtctgacatc attgatacac ttgaaaacaa caaacataaa
     8821 ccagacttat cactatatga tctaaataat tcactttcat tttaagagtg aaagaaacca
     8881 agctatttaa gctgtgtaca gctttaaata aacaaaattg aatgggagag ttttttaaat
     8941 gaaaaaaacc aaaatcgtta gtacattagg accagcaagt aactcagtag aaattatttc
     9001 tcaattgatt gaatctgggg caaatgtctt ccgctttaat ttttctcatg gtgaccatga
     9061 agaacaatta tcacgtatga ctatggtccg tgaagcagtt aaaaagacag gaaaagatgt
     9121 cggtattctt ttagatacaa aaggagctga aatccgtaca actgttcaag gcacaacaga
     9181 agctgatttt ggtcgtgcag gatacatcaa atttgaagta ggagatcaaa cacgcatctc
     9241 tatggatcct gaacatgtag gttcaaaaga aaaaatcgcg gttacttacc caggattatt
     9301 tgacgatgtt cacgttggcg gacacatttt atttgatgat ggtttgatcg atatgcaaat
     9361 cattgaaaaa gacgaagcaa accgtgaatt agtcgtagaa gttaaaaatg caggtatgct
     9421 aggatcacgt aaaggggtaa atgcaccagg cgtttctatt agtttgccag gtattactcc
     9481 aaaagatgct gacgatattc gttttggttt agacaacgat atcgacttta tcgctgcaag
     9541 ttttgtacgt aaagcacaag acgttctaga tatccgcgaa atcttagaag aaaaagacat
     9601 gactcatgtt caaatcttcc ctaaaatcga atctcaagaa ggtatcgaca acattgacga
     9661 aatcatcaaa gtttctgacg gtgttatgat tgcccgcgga gatatgggtg ttgaaatccc
     9721 agctgaacta gtaccaatgg ttcaaaaacg cattatcaaa aaatgtaatg ctgctggtat
     9781 cccagttatc actgctacac aaatgttaga atcaatgcaa gaaaacccac gtccaacacg
     9841 tgcggaagct tctgacgttg ctaacgctgt atttgatggc acagatgcaa caatgctatc
     9901 tggtgaatct gcaaacgggg actacccagt tgaagctgtt tcaacaatgg cacgtatcga
     9961 ccaagaagct gaaaatgcat tatcagaatt aggtacattc caattgaacg aattcgataa
    10021 aacagatgta actgaaacaa tcggtttatc tgtagctcgt gctgcaaaaa atctaggcgt
    10081 gaaaacaatc gttgctgcaa ctgaatcagg atacactgca aaaatgattt caaaatatcg
    10141 tccagatgca gatatcttgg ctgttacttt tgacgaacgc acaaaacgtg gtttgatgtt
    10201 aaactggggc gtttacccaa cagttgctga aaaaccatca acaactgatg aaatgtttga
    10261 actagcagct aaaaaagctg ttgaattagg tttcgcatca gaaggcgatt taatcttgat
    10321 cactgctggt gtaccagttg gtgaacgcgg aacaactaac gtgatgaaaa ttcaaatgat
    10381 tggttctaaa ttattagaag ctcaaggcgt tggacaaaaa tctgtcgttg caaatgcagt
    10441 tattgcttca tctgctgaag aagctgtaaa aaaagcaaaa gacggaatgg ttctagttgt
    10501 accatcaact gacaaagaat atatgcctgc aatcgaaaaa gcagctgctg ttatcgttga
    10561 agaaggcgga ttgacttctc acgctgcagt tgtaggaatt gcacaagata ttcctgtaat
    10621 cgttggtgct aaaggcgcaa caacaacaat tacagaaggt gaattgatta ccgttgattc
    10681 acgtcgcggc atcgtttacc gtggcgaaac aacagctatc taatttagct taataaaaaa
    10741 taaaaacagc tcttctttta cgaagagctg tttttttgtg ctgtaacgaa ttcatggata
    10801 acttggacta cttgttcttg aagaggcgtg acgatctgct gtgttcgttt tgccaatgaa
    10861 atcaagaagc gaggctgaac ttcatctgaa agagggaggc aaactaaatg atcttcagga
    10921 tggacagcaa tctctgccaa aaaaccgata cccacatttt ctcggaccat actctttaaa
    10981 atcgtcaagt cattgctttg ataaacaact tttggatgag tatgtgtttg tttagaaagc
    11041 ttagtgaatg ctgcggggtg gacataatgt tcgttcaata gtacaaactg ttcatctgct
    11101 atatctttga aagagacagt atcctgcttg gaaagaggat gtgtaggtgg cacgacgatc
    11161 atgaaacgtt tgtccaaaag taactctgtt tctaaatatt gatccacgat tggctgtgtt
    11221 gagcctaata cagctaaatc gatcctccct tgtcggatca tcccgtataa gtcccgagag
    11281 cctccatcgg taatctcaat ttgattcatc aattcatgct tgataaagta acttgacagt
    11341 ttaggaaagt attcattgcc gataatagga ggcatgccaa aacgaagctt tttcttttgc
    11401 aactgagtga tttcagctct tgcagtagaa atctcttgga gaatggtttg tgcatggaga
    11461 aacagctgct ttccagaagg cgtgacgatt aattcgtgat gcgatcgatc tcttgtaata
    11521 agtacggtgt caaactcttt ttcaagacgt tgtaatgcat atgtaatagt aggttggctc
    11581 acacggaaat aattagccac tttcgtaaaa tttttttgtt tgacgatttg gacatagtat
    11641 tcgatatctt tcgtgttcat gaagatcatc cgctccttta atggaataat gattattgta
    11701 aatgattctg aggatgatag caaacagagg ctttctaata aagatttttc tgttttttta
    11761 tgaactgcaa ggtgtcaaga aaatttcatt gacaacatgc tttttgccct gtataattgc
    11821 ttttgttgct taggagtgat gaaaaatgaa atggtcttta ttggaattga gaaaatacca
    11881 ggagacaccg ttaacttttc atgagacgct cgacttgaaa gccgctctca tgaagcgaga
    11941 caatttaata cttgatgttg cgccagtgga agtcgaaggt ttggtctcag ttgataagaa
    12001 aggctatatc gttcattaca ctgtgcagac gactttgaca gttccttcaa cacgttcact
    12061 agaacctgtt gaactgccca tggatttttc tgtagacgaa gtattcatga cagaagaaca
    12121 attccaaagt agagatgaac aaatgtctgc ggatgagatt ttgctaattg aaggtccatt
    12181 attgaattta gcagagtcga ttgaagacaa catcttattg gcgattccaa tgcgggtatt
    12241 gacaaaagaa gaggaagcag caacagaact tccaaaaggc aatgattggg aagttgtttc
    12301 cgaagaagaa taccagcgca gaaaaatgga agaagccgag aataaaattg atcctcgtct
    12361 tgcaaaacta tctgaacttt ttgattctaa atcaaaagac gacgataaca agtaagattg
    12421 gaatatattg tgtctaattt agatacaaga ctttacaagg aggtgtagca aaatggcagt
    12481 accagctaga agaacatcaa aagctaaaaa agcaaaacgt cgtacacact acaaattgac
    12541 aatcaaaggg ttaaacgaat gtccaaactg tggtgaaatg aaaaaaagcc atcacgtatg
    12601 tgcaaattgt ggttactatg atggtaagga cgtaatgtca aaagaagctt aatacatttt
    12661 aattagaaaa tgtaaaacct caaggctaac catggagcct tgaggtttat ttttttgtag
    12721 tcggtaaata aaaaatatgt tgttctttag tttgcaatgt agccgtgtta agctataatg
    12781 taagagatta gggagaaatc ccgtatgtag gaggagttca tatgtcaaaa caacaaatcg
    12841 gcgttgtcgg tatggccgtt atgggtaaaa atctagcctt gaacatagaa agccgcggct
    12901 attcagtggc tttattcaat cgtacagggg ctaaaacaac cgctgtagta gaagaacacc
    12961 cagataaaaa tttcaaagca acatatacaa tcgaagaatt tgtagaatct atcgagaaac
    13021 cacgtcggat cttgctaatg gtcaaagcag gaccggcaac agatgcaacg atccaagagt
    13081 tgcttcctca ccttgataaa ggcgatattt tgattgacgg aggaaataca ttctttaaag
    13141 atacgatgcg tcgtaatgaa gagttagcca actctggcat caactttatc ggtacaggcg
    13201 tttccggtgg tgaagaagga gctttaaaag gaccttcaat catgcctggt ggacaaaaag
    13261 aagcctatgc gctagttgca ccaatcttag agcaaatttc tgcaaaagca gaagacgaag
    13321 caccatgtgt cacatatatt ggcccaaatg gagccggaca ttatgtgaaa atggtacaca
    13381 acggtattga gtatggagat atgcaactga ttgctgaatc ttatgactta atgaagaata
    13441 ttttaaacct ttcagtagaa gaaatggctg atatctttaa agaatggaat caaggcgaat
    13501 tagacagcta tttgatagaa atcactgctg acattctgac acgtaaagac gatgaaggaa
    13561 caggtaaacc agtagtagat gtgatccttg atgcagctgg gaataaagga acaggtaaat
    13621 ggacaagcca aagcgcgctt gacttaggtg tgccacttcc attgatcact gaatctgtat
    13681 ttgcccgcta tatttctgct tataaagaag aacgcgtaca agcaagcaag attttatctc
    13741 gtacgaacga ttttgagttc acgggagaca aaaaagaatt ggtcgaaaaa atccgagaag
    13801 cgttgtattt cagtaaaatc atgagttatg cccaaggatt tgctcaatta cgtgtcgctt
    13861 caaaagaatt tgattgggac ttgccatttg gtgaaatcgc aaaaatctgg cgcgctggat
    13921 gtatcatccg agctcgattc ttgcaaaaaa ttacggatgc ctatgacaaa aatcctgaga
    13981 tcgaaaattt attgctagat gattacttcg ttgaaatcac gaaaaaatat cagcaatcag
    14041 tcagagacgt cgttgccttg gctgtccaag ctggcgtacc tgtaccgact ttctcatcag
    14101 ccatcgctta cttcgattca tacagagcag aacgtctgcc tgcaaatata atccaagccc
    14161 aacgcgatta ctttggcgca catacctatg aacgtgtcga caaagaagga atcttccatt
    14221 actcatggta ccatgaagaa taatagtctt tcggctgtta ggaaatgaga aaataaaaag
    14281 aggacgaaag agctgaagtc gattgttatg agtagacttc agctcttttt taaaattgct
    14341 atccatgata aaatgactat agatttttaa gcatattatc ggtataatga tagagttttt
    14401 aaaaagccag tagaatagaa aggataaaat aataaatgag caatattcta atcattgaag
    14461 atgaaaagaa cttggcacga tttgtcgagc tagagttgaa gcatgaaggg tataacgcag
    14521 aagtccacta caacggccga actggtttag atgcggcatt aaataatgag tgggatgcca
    14581 tattgttgga tctgatgctc ccagaattga acggtttaga agtctgcaga cgtgtacgcc
    14641 aagtcaaaaa tacaccaatc attatgatga ctgcaagaga ttctgtgatc gatcgtgtat
    14701 ctggacttga ccacggagca gatgactaca tcgtcaaacc atttgctatc gaagaattat
    14761 tagcacgtct tcgtgcattg ttgcgtcgaa tcgatattga aggcgataaa aatgtggcta
    14821 aacaaactac tattacttat cgcgatttga ctatcgaaaa agaaaaccgt gtcgtacgtc
    14881 gcggaaatga agtaattgaa ctaacaaaaa gagaatacga gttgctgctt actttaatgg
    14941 aaaacgtgaa cgtggtatta gcacgtgacg ttttactaaa taaagtgtgg ggatacgaaa
    15001 cagaagttga aacaaacgta gtggacgtat atattcgtta tcttcgcaac aaaattgacg
    15061 ttcccgggga ggaaagctat atccaaactg tacgcggaac aggttatgtg atgcgctcgt
    15121 gataaagccg aaaaaatcgg aaaaaagaga actgaaaggg ccctccttga caatcaagtg
    15181 ggcctttgcg agttctttct tcatattcgt tgtgtttaca attttcgctg tcattaccta
    15241 taaatcatct atcaatctga ttgtggcaaa agaaagaaac aatgtagagc gtacgatttc
    15301 agaagtagct tctcgtttgg ctaactcgga tgaagaactg acattagtaa atacgtatcg
    15361 taatctgacg aaagcgacaa atgacggaga tgaggtcgta gatcatactt cactggtcga
    15421 aggaaactta atgaagatcg attcctttat agctgagcta gggcagccat caatggatct
    15481 ttatgtttat gatttggacg agaacttgat ttttaaaacc catgaaaaca gtcgaaacct
    15541 gattcagact acacgtaaag ttccaacgat cgttacacta gaaggaaaaa ctggcttttt
    15601 atctatccag cctatatatt ctaaaggtac aagagaaaag atcggctatg cgcaaagctt
    15661 ctatgaatta tcttctttct atgatattcg aaataagcta ttattgactc ttattgtttt
    15721 agaagttgtt tctttgatct taagtagtat tttaggtttc tttttatctt cttatttctt
    15781 aaaaccactc aaggtactca gagatacaat ggatactata cgtaaagatc cgcaatcaga
    15841 tatccacatg cctgaaatcg acacaaatga tgaattggca gatttagctg aaatattcaa
    15901 tgagatgctt gaccgtatgc gtttgtatat tgaacaacaa gagcaattcg tggaagacgt
    15961 atctcatgag ttaaggacac ctgttgcaat tattgaaggg catcttagtc tgctaaatcg
    16021 ttgggggaaa gatgatcctg agatattaga agaatcatta agcgccagca tgcaagaaat
    16081 tagccgtatg aaaagcttag ttcaagagat gctagactta tcaagagcag aacaagtaga
    16141 tgttcattat gcgaatgaaa caactaaagg aaaagaagtc acctatcaag tttataataa
    16201 tttcaagata ttatatccag actatgtgat cactttagat gatgatttga caaaagaagt
    16261 gaccctgcaa atttatcgta atcactttga acaattaatc attatcatat tggataacgc
    16321 agtaaagtat tcaactacac gaaaagaagt ccatatctct atttcttcca ctctgaatga
    16381 atttgagatc gcgattcagg actttggcga aggaataccc aaagaagacc ttaataagat
    16441 cttcaatcgt ttttatcgag tggataaagc aagagctaga acgaagggtg gaaatggtct
    16501 ggggttgtct atcgctaaac aactggttga aaactataaa ggacgtatct tagcagaaag
    16561 tgtagtaggg caagggacaa tttttagaat ctttattcct atcgcaaagg cagaagacaa
    16621 gtaaagcatg tgctttactt gtttttttat atatccaaca aagaataagg tacaaaatac
    16681 aagcaagtta gaaattcagg gatgtcatta taaaaaaaac tagcgaacaa ttaaatagaa
    16741 taacatccgt taattaaacg taagtagctt ttagatgaac gaaaaaacaa tgaaatgctg
    16801 taatattcgt gtttaaaaat aataaataaa aatagggaat tttctattgc aattttataa
    16861 agagattgct atattattat ttgttctaaa tgattccttt aaggtgtcac taaaaattat
    16921 aaaaaatagg gttgacgcac ttttgatgtc atgttataat aacgaagtcg tttggatgag
    16981 gtagctaata aagcttgatt ttatcgaaaa aaacttcgaa aaagttgttg acaaataaca
    17041 ataaagatga tatgataatc aagtcgctgc taatgagtga caaagacgtt tagaaaaaat
    17101 aaacaaagaa gttgttgaca ttctcttctg aatctgataa gattaaagag ttgttgaaac
    17161 aaaaagatta gacctttgaa aactgaacaa agtaagacaa accaaatgtg tagggcgtct
    17221 tgattcaatt caagacaaca aacattttta acaagcaagc aatatgctag caaacaaatt
    17281 gagcttaaca atcgcaagat tgttcgaact ttttatgaga gtttgatcct ggctcaggac
    17341 gaacgctggc ggcgtgccta atacatgcaa gtcgaacgct tctttttcca ccggagcttg
    17401 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca
    17461 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt
    17521 ttgatttgaa aggcgctttc
//
LOCUS       DAPBCS010000059        17280 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPBCS010000059 DAPBCS010000000
VERSION     DAPBCS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.061"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            109..888
                     /locus_tag="RD521_002350"
     CDS             109..888
                     /locus_tag="RD521_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772034.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTIVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIDQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            934..1878
                     /locus_tag="RD521_002351"
     CDS             934..1878
                     /locus_tag="RD521_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDT7772035.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1894..2673
                     /locus_tag="RD521_002352"
     CDS             1894..2673
                     /locus_tag="RD521_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDT7772036.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKEAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNGKTAPSRRSRSRV"
     gene            2685..3383
                     /locus_tag="RD521_002353"
     CDS             2685..3383
                     /locus_tag="RD521_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDT7772037.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQIFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLSKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3411..4175
                     /locus_tag="RD521_002354"
     CDS             3411..4175
                     /locus_tag="RD521_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDT7772038.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFRKNPNYLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIGKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4188..6005
                     /locus_tag="RD521_002355"
     CDS             4188..6005
                     /locus_tag="RD521_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDT7772039.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            6059..6982
                     /locus_tag="RD521_002356"
     CDS             6059..6982
                     /locus_tag="RD521_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDT7772040.1"
                     /translation="MNAFKTALNNNEMEGKIIGADCSELAPALYMCDEKIVLPKVSES
                     NYIEELIKVIKFFEIKLIVPTIDTELSTLSRNKKYIENETDSVVLVSSEKVIDICENK
                     ILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSSSVNTFIVNNEKELLFFSEYID
                     NPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATRGGEILKGQIIKDRELIETTKK
                     MLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPISIKSGANSPEYIIKMIDSKKN
                     LRYRENYLDKMIALRYDQAIYLDEKGNVYND"
     gene            6954..7670
                     /locus_tag="RD521_002357"
     CDS             6954..7670
                     /locus_tag="RD521_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDT7772041.1"
                     /translation="MKKEMYIMIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIF
                     PATFDEIYRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLEN
                     ADTLLTELRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPI
                     SYLLLLEKFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFY
                     QADKKIRSLWEIKNFLGVIS"
     gene            7667..8785
                     /locus_tag="RD521_002358"
     CDS             7667..8785
                     /locus_tag="RD521_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDT7772042.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            8775..9395
                     /locus_tag="RD521_002359"
     CDS             8775..9395
                     /locus_tag="RD521_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDT7772043.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            9522..10691
                     /locus_tag="RD521_002360"
     CDS             9522..10691
                     /locus_tag="RD521_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDT7772044.1"
                     /translation="MCRVLNILFQYEKIIKKYKEKRVMAKYYNKKILIMANNDDGLYR
                     FRGMLIKNLVDQGNEVIVSLPKGKNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNY
                     CNILKKIKPDMVIAYTIKPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFL
                     YRLAAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVH
                     FLFIGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYH
                     GYQSDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGY
                     LCERKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            10698..11093
                     /locus_tag="RD521_002361"
     CDS             10698..11093
                     /locus_tag="RD521_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7772045.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            11090..12091
                     /locus_tag="RD521_002362"
     CDS             11090..12091
                     /locus_tag="RD521_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDT7772046.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKIGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKNKIINLIYPKLIEDEHGE
                     YFSPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKGISAKFALKNKWVFLIYIYNKLIKR"
     gene            12097..13143
                     /locus_tag="RD521_002363"
     CDS             12097..13143
                     /locus_tag="RD521_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7772047.1"
                     /translation="MSVKISVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTD
                     NTLAILKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFES
                     LYNCAIAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNR
                     LYSHNAWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYR
                     QQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQK
                     NVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKM
                     YKTAYNMLWRIYNR"
     gene            13157..14236
                     /locus_tag="RD521_002364"
     CDS             13157..14236
                     /locus_tag="RD521_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7772048.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQTPDAKKYFSKINDKKVK
                     TIIIPNPIKENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYECELDIY
                     GEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNSMLEAL
                     AIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVVRKAQK
                     GIKLRKELTSKNISNIWYEYLNQFF"
     gene            14279..15679
                     /gene="wzy"
                     /locus_tag="RD521_002365"
     CDS             14279..15679
                     /gene="wzy"
                     /locus_tag="RD521_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HDT7772049.1"
                     /translation="MFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAICTWHWRKGD
                     SIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSESDMIMVKWY
                     SCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIVVPIVFVSK
                     LLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKVITRFVFII
                     FALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIGIYILNVIT
                     KARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNGIYPFANTY
                     LTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNGGYIGGIVY
                     IFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIVKNFVYGVL
                     IILLINFFLQQFNKRL"
     gene            15725..17230
                     /locus_tag="RD521_002366"
     CDS             15725..17230
                     /locus_tag="RD521_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDT7772050.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWARKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYRRYVVKIISKIIVKIKKI"
BASE COUNT         6312 a         2415 c         3327 g         5226 t
ORIGIN      
        1 tctgtgaata tattagttta attatgtttt ttaaaataaa aagctgtcat ttttatgtaa
       61 gtgaattata tcttaattta tctatcatca atacttagga gtgaatctat ggaaaagcca
      121 ttcgtcaagt cattgactgc tgtggcggta ggtgcaggtg ctattgcgat ctgtttgttt
      181 ggttaccata tcaataatca gcggcaacat cagcaacgga tcaattatgc agaatctgcc
      241 atcaccaatc aaaaagacac cttgacttct ttatcaaaag aagtagacaa gctgtatagt
      301 acaaaagaaa aaaattttct aaaccctgaa ataacagaag aaacaatcac aagtttgtca
      361 aataagctat cctctgtcaa actttcagca gatgattacg acatcaaaga atcagaattg
      421 cctaaagaag ctgttgcaat ccaagatgag aagaaagcaa tcgtgacaca gttagatagt
      481 gcagaatcaa aactgaagat ccagacagct gtcaacgatt tatttactaa aaatgtatca
      541 aactggcagc aagctgtaga cgatgtcgtc atcaaagaaa aggtgacaag tgcagatgtc
      601 gctcgtgtac gtgaaaatat gagctttttt aaagatagtc cttggaaaac gattgttatg
      661 caatatctag gattcgctga tactcagatc gcgcaagtca cgcaactaga tcagttattc
      721 gatacgatgt tgaaggatgg tcaagtgact gccaccgcta cttacgatca atatttgacg
      781 gtgctcagtc aaatagatca gatccggaat gaaaaaatac gcgcagcata cgcaacaaaa
      841 gcacaaactg ttgctcagca aatgggctat agcaatataa gctattgaca gttaaaacgt
      901 tgattcaagc aaggttagca aacggaggaa actatggcaa gacgaagaag aaaatcaaga
      961 gggaaaaaaa tatttctgat tattttagca gttcttatcg tattagtatt agcaattgta
     1021 ggagtaggag caaaattata tctggatctc tcacattcta ttgagaaaac ttacgaatca
     1081 gtggaacgaa cccaaggaga acgaacgaaa ccagttgatc tggacaaaca agaatctttc
     1141 tctgttctat tgatgggcat tgatactgga gatttaggtc gggtcgatca aggaagatca
     1201 gatacgatga tggttgccac agtcagtcct gaagacaaac agacaacgat cgtcagcatt
     1261 cctcgagata cgtatgtcga catcgtaggt cgtgacacaa aagacaagat caaccacgct
     1321 tacgcattcg gcggagctgc catggcgatg gataccgtac aaaaatatgt agacatccca
     1381 atcgatcact atgtctctat caacatgaaa gggctaaaag aacttgttga tgcagttggt
     1441 ggtatcgaag tcaataatga cctcactttc tcacaagatg gctatgactt tacaatcggt
     1501 aaaattactt tgaacgggga tcaggcatta tcttattctc gtatgcgtta tgaagatcca
     1561 aatggtgact atggtagaca agagcgtcaa agaaagatcg tagaaggtgt agcgaaaaaa
     1621 gtattgagtc taaacggtgt cagcaactat caatccgttt tgaatgctct agagtcgaat
     1681 atgaaaacag acatgagctt tgatgaaatg aaaaaaattg ctttagatta tcgggaggca
     1741 tttggtacga tcaagcaaga tcagctgcaa ggagaaggct tcatgcaaga taatatctcc
     1801 tatcaaaaag tcagcgatca agaacttgat cgtgtgaaaa aagaattaaa agatcaaatg
     1861 aatctagaga ataaatagga aagtggagaa aaaatggaag agacaatcag cttgaaagaa
     1921 atttttgaaa cattacgcaa acacttggta acaatcatca tctcaatgtt tgctggcttg
     1981 gccatctcag gaatcgtaac atttttcatc ttgacaccaa aatacagttc acaagcacaa
     2041 ttgatcgtga cactgccaca atcagacaca gcaaatgtca atgatgtcaa tacgaacctg
     2101 caaatgatca atacgtataa agatatgatc gtcagtgatt tagtgctaaa tgaagtcaaa
     2161 gatcgtttag aaacagaaga caatgtgaaa atgactgctg aacaaatcaa agaggccatc
     2221 tcagtcaatc agtcacaaaa ttcacaaatg ttctcgatcc aggcaataag cacgaatgca
     2281 gtgactgctc aacagatcgc taacacaact tcacaagttt tccaagagaa cgcaaaagac
     2341 gtgatgaatg tcgataaaat ctcgattatt tcaggtgcag ttgccaatac gacacctgtt
     2401 tcgccaaata ataaattgaa tttagtgatt ggcttagtat taggaatgat ggtaggcgta
     2461 ggtctggcat tcttattcga attactcgat cggacagtga aagatgaaaa atatgtgacg
     2521 gatacattag gattcccaat cttaggaaca gttccagaaa tgtcagcgaa agaattgaac
     2581 gctggtatcc gtaaaaaagg atcagctcct gcacgaccaa aagctacaaa tggtaaaaca
     2641 gcgccatcac gcagaagccg ctcaagagtc taggaggaag aaaaatggca cgaacacaga
     2701 aacaaaaagc agcacaaact catgcagtca gtttgatcac attaacaaac ccatcttcac
     2761 caatcgccga acaatatcgt acgatccgga cgaatatcca gtttgcctcc gcagctggtc
     2821 agcaaatcaa aacgattgtc gtgacttcat caggtcctgg agaaggaaaa tccaccactg
     2881 cagcaaacat tgcagtcgtt tttgctaaat ccggtcaacg ggttctatta gtcgatgcag
     2941 atttacgtaa acccgtcgtc tataaaacat tccagttgag caatgctagt ggattaagta
     3001 cagcacttag ttcatcagga agtgtagcag atgtgatcca acgaacgcca gtcgagaact
     3061 tgtcgatttt gcctagtggg cctaagccac ccaacccatc agaattgtta agctcgccaa
     3121 gaatggatca gattctagcc gaagcaagac agatatttga cgtggtgata ttcgatatgc
     3181 caccagtagt agccgtaaca gatgcacaga tcatgtcttc aaaaacggat ggcactttat
     3241 tggtcgtacg tgaaaataca tcacgtaaag aatccttgtc taaagcaaaa gaactgctag
     3301 agatggtaca agcacgagtc ttaggtgttg tatacaacgg agcagaacat agcaaagatg
     3361 ctggttatta ctacggaaat taaggaataa gaagagagga gacaagagaa atgatcgatc
     3421 tacattgcca tatcctacca ggaatcgatg acggggcaga aaacttagaa gcaagtattg
     3481 caatggctga aaaagcgatt cagcaaggca tcacgcatat tttatgcaca ccacaccaca
     3541 ataatggaaa atacagcaat gaaaaatcac aagtcatttc tctagtcgct tcgttacaag
     3601 ccgaattaga gaaaagacaa ttgcctttga cgttattgga aggacaagaa gtacgtatta
     3661 ccggaacgct catcgaagac atccaccgag atgaaatttt atttacagat ttagatgata
     3721 catatcttct gatcgaattt ccgacactgg aagtgccgtt atatgcagaa cggctttttt
     3781 ttgagttgtt agaactagga aagacaccag tgatcgtcca tccagaacgt aacgcgcatt
     3841 ttcgtaaaaa cccgaattat ttgcttccgt tcctagatat ggggtgtttg gcccaactta
     3901 ctgcgccaag ttatgttggg gtgtttggca aagacatcca gaaaacagct aaaacgatgg
     3961 tcaaacataa tctcgtgcag atggctgctt ctgatgccca tggggtctct aagcgcacct
     4021 tttacttaaa agagtgctat gagcagattg gcaaggactt tggtaaggaa aaagtagtac
     4081 agatgaaaca agtcgcaaag gatctgatca acggggatga aatcagttat ccagtctatg
     4141 aagaggtaaa aaagaagaaa tttggtttat tttaggggga atgtagggtg tttgtattaa
     4201 caagaagaag aaaaattgcc acattggttg ttgtggacag cttgttatta atcgctgcaa
     4261 atattgcagc agctaaattc atgaagccgt ttgtagctat accaatggac cttattttaa
     4321 tctctattgg tttgtcgatt ggtttttatt tattttatgg tagtttattt aaagttttca
     4381 ctcgaatcaa tcgctataca aatttaagag agatcattgc aatttttagt tcactatctg
     4441 caagtgctgc ctcaagtatc ctcatactgc tgttcattaa tcggcgatac agtttacggt
     4501 tagttatctt tgcttatctg ctttcgctct tattgatcat cggtagccga ttgatctggc
     4561 gtatctacat cgaaacaaaa aatatgagat atgtatcagc agattctgct aaaaatacat
     4621 tgattgttgg tgctggcgaa ggcgggcgta ttttatacaa cagctttctt ggatccaaaa
     4681 cggctcaaga tatccacgtt gtcgggttcg tagatgatga tccaaataaa agaaatacgt
     4741 atctttcagg aaaaaaagta ttaggtgcat taaaagatat tccggaattg attgaaaaat
     4801 atgacattca aatggtaacg atcgctatcc cttccttatc aagaaaaaaa ttacgaagaa
     4861 tcttcgagtt agtcgaatcc gctcgtgtga aagtcaacac gatgccttct atagaagaac
     4921 ttgcttcagg aaaaattagc gtatcaaaat taaaaacaat tgacgtagtg gatttactgg
     4981 gaagagatga ggtaaagctg gatattgaat ccatcaaaga tcagatcaca gacaaagtca
     5041 ttcttgtcac cggcgcagga ggatcgatcg gttcagaaat ctgtcggcaa atcattcaat
     5101 tcaatcctgc aaagctattg cttttaggac atggggagaa ctcgatctac ttgattgacc
     5161 gtgagttacg cacgcatcat caaaattgtc ctacagaaat cgtaccgatc atcgcagata
     5221 tccaagatcg cgagaaaatc aatgagatca tggaacaata tcatccagat atcgtctacc
     5281 atgcagctgc tcataagcat gtaccactga tggaatataa tccaaaagaa gcagtcaaaa
     5341 acaatatttt cggtacaaaa aatgtagcag aagcagctaa agcagcaaaa gtgaaaaact
     5401 tcgtcatggt atcgacagat aaagctaaca atcctccaaa cgtcatgggt tcaacaaaac
     5461 gaatcgctga aatgatcgtg actggattga atgaagaagg ctgtacgaaa ttctcagctg
     5521 tcagatttgg gaatgtcctt ggttctcgcg gtagtgtgat tccagtattc agagaacaaa
     5581 ttgctcaagg tggtccaatc actgtaacag actttcgtat gacccgatac ttcatgacga
     5641 ttcctgaagc gagtcgttta gttattcaat ctggcgcatt agcaaaaggt ggcgaaattt
     5701 ttattctaga tatgagtgaa ccagtgaaga ttgtagacct agccaaaaac atgatccgat
     5761 tgagcgggta tagcgaagac gaaatcgaaa tcattgaaac aggtatccgt ccaggggaaa
     5821 aactatatga agaactattg ttggataagg aaagaaatga tgaagcagtt tatgagaaaa
     5881 tctttgtagg aaacatcaaa ggttactcaa tacaaaaagt gatggatttt gttaagagcc
     5941 tgccacaaga cgatgaacaa ctggctaaag acattgtcac ttttgcaaat gcttcgaata
     6001 gataggagat taaaaattga atattttaat attaagttgt ggacgtcgtt gtgaactagt
     6061 gaatgcattt aaaacagcat taaacaataa tgaaatggaa ggaaaaatca ttggagcaga
     6121 ttgctctgaa ctagcacccg cactatatat gtgtgacgaa aaaatagtat tacctaaagt
     6181 ttcagaaagt aattatattg aggaattaat aaaagtaata aagttttttg aaattaaatt
     6241 aattgttcct actattgata ctgaattatc aacattatca agaaataaaa aatatattga
     6301 aaatgaaaca gactcagttg tattagtttc ttcagaaaaa gtcatcgaca tttgtgagaa
     6361 caaaatttta acttctgctt tttttgaaca aaatggtttt aaaactcctg aaattttaaa
     6421 ctcttctgaa attacaaaat ttcctgtatt tataaaacct ctgaatggta gttcatctgt
     6481 taacacattt attgtaaata atgaaaaaga attgttgttt tttagtgaat atatagataa
     6541 tcctcttatt caagagtata ttttaggtga agaatatact atagatgttt gcttaaactt
     6601 tgattcgaca ccagtaagta ttgttcctag aaaaagaata gcaactagag gtggggaaat
     6661 actgaagggt cagattatta aagatagaga actaattgag actacgaaaa agatgttgga
     6721 aaaattaaat gcaatcggac atattactat tcaatgtata aaaaacgaaa agggaatatt
     6781 ttatttagaa ataaatgcaa gatacggtgg aggagccccc attagcatta aatcaggagc
     6841 taattcccct gaatatatta taaagatgat agattcaaaa aaaaatttga gatacagaga
     6901 aaattattta gataaaatga tagcactgag atatgatcaa gcgatttatt tagatgaaaa
     6961 aggaaatgta tataatgatt aaagcggtga tctttgattt agatgacact cttgtgtctg
     7021 aatatgatta tgtaaaaagt ggatacaaat gtgtagctag aagattagca gtgcaaaaaa
     7081 ttttcccggc tacttttgac gaaatatata gagtcctttt agatcttttt gctgaagata
     7141 gtaaaaatgt atttaatcgt ctatatgact attttgaaat tccttatagt aaaaaagaaa
     7201 ttaaagaact ggtaaatgta tatcgagaac acaaaccaga cataaatttt ttagaaaatg
     7261 cagatacttt attaacggaa ttaagaagta gaggatacaa gctggggatt attacagatg
     7321 ggtatgctat aacccaacga aacaaattgg atgctttgaa agtagactat ttggtagatt
     7381 atatttgtat tactgacgaa ttaggccgtg aattgtggaa accacaccct atctcatatc
     7441 ttttattgtt agaaaagttt aatttaaagg cacaagagtg tatatatatt ggcgataatg
     7501 agttgaaaga ttttataact gccaaatcaa taggaatgca aacaattaaa atagaaagag
     7561 taaatggtat ttacagagaa agcagtttga agttcaaaag attttaccaa gctgataaaa
     7621 aaatacgttc gttatgggag ataaaaaatt ttttgggagt gatttcatga aaagtaaaag
     7681 aattctatta gcttctccac atatgagtga agaaggatat gaaaaagagt atgtaaaaga
     7741 agcatttgat acaaattggg ttgctccttt aggtgcgaat gtgaatggat ttgagaaaga
     7801 attagcagaa tacgtgggag ctagtcatgc tgccgcatta tcttctggaa cagcagctat
     7861 acatttagct ttgaaagcgg ctggggtcgg taaagatgac atagtgattt gtcagtcctt
     7921 gacgttctca gctactgcta atccaattct ctatcaagga gctatacctg tatttgttga
     7981 tagtgattac gaaacttgga atatgaaacc tgaacatttg cgaacagcac tagaaaaata
     8041 tcctaacaca aaggctgtat tagtagtaca tttgtacggt ttatcagcta atttagacga
     8101 aattactaaa atatgcaaag aatacgatgt tactctaatt gaagatgctg cagaatcatt
     8161 aggaacaaca tataaaggaa aatatactgg aacatttggc gactatggaa tttattcttt
     8221 taatggaaat aagataatca caacttcagg tggcggaatg cttgtatcca atgacaaaga
     8281 aaaggtggaa aaagtgagat tttgggctac gcaatctcgt gagaaggcac gccattatca
     8341 acacaatgaa attggctata attatcgaat gagcaatatc gttgctggta tcggtcgagg
     8401 acaattgaaa gtattaaatg aaagaattga gaaaaaacga tatattcata gtttttataa
     8461 aaaagaattg tctaaattgt caaatataca attcatgcca gaaaataatt ggaatgaacc
     8521 gaattattgg ttaagtgtca taacaataaa gggaataaat gcgaatcatc ttattgatga
     8581 gttagacaaa aataatatag aagcccgccc agtatggaaa cctatgcatt tacaacctgt
     8641 ttttaaaaat tatgattata ttggcgatga tgtagcagaa actttatttg acttaggagt
     8701 atgtttacca tcagatacaa agatgacaga tgaagattta aaatatgtag tatctacgat
     8761 taaaagagtg atcaatgatg cgtaaaaaaa ctttctatca gctttttggg aaaagaattt
     8821 tagatatcct attgtcagga attgctctca tcgttttatc gccgataatc ttaattgtag
     8881 gttttttagt aagaattaaa cttggttctc caattatctt caaacaagaa cgaccaggaa
     8941 aaagtgaaaa aattttttcc atgtataaat ttcgtacaat gactgacgaa agagaccaca
     9001 atggagaata tttacctgat gaaattcgac taactaaatt tgggaaaatg cttagagcaa
     9061 caagtctgga tgagctaccc gaactttgga atattttgaa aggtgatatg tcgatcgtag
     9121 gacctcgtcc attattagtg gagtatttgc cactatatag tgagaagcaa cggaaaagac
     9181 acaacgtaag accaggatta actggactgg ctcaggtcaa tggaagaaat gctattagtt
     9241 gggaagaaaa gtttgattta gatgtttatt atgttgataa aattagtttc tttaatgatc
     9301 ttattattat aattcagaca tgtaaaaaag taatcaagaa agagaatatt aatacaatca
     9361 ataacaatat cccagaaaaa tttaaaggga gttaaataca gcagcttaat gcaagttttg
     9421 tgacttgcat tatatttagg tataaatcaa agcgagtata ttagaattat ataacatatg
     9481 ttaaaaaaac gtatagctaa taaaagtatg attttaaaaa tttgtgtaga gttttaaata
     9541 tactgtttca atacgagaaa attataaaaa agtataagga aaagagagtt atggcaaagt
     9601 attataataa aaaaattctt ataatggcaa acaacgatga tggtctttac cgttttcgag
     9661 gaatgctcat aaaaaactta gtagatcaag gtaatgaagt tattgtatct cttccaaagg
     9721 gcaagaatat tcctaaatta attgaacttg gatgtcaaat tattgaaact ccaatagata
     9781 gaagaggaat taatccaaaa actgacttta aactattttt taactattgt aatattttga
     9841 aaaaaataaa gccagatatg gtcattgctt atactatcaa accaaatata tacggtggat
     9901 tggcatgtcg gttaagaaag gtttcttatg cattaaatat aaccggatta ggaacggcat
     9961 ttcaaaaaaa gggggttttg aaaagtctca ttgtattttt gtatagatta gcagctaaaa
    10021 aatctaaggt catatttttc gaaaattttg aaaatatgaa aacatttcag actttacaaa
    10081 ttgctaaaca aagtaaatac aagctattaa acggtgcagg agtagatgta gaacacttta
    10141 gttatttaga atatcctaaa gatgagcaag ttcatttttt atttattgga agaattatga
    10201 aagagaaagg tgtagatgaa ctttttgaag caatgaaaaa cttatatagt gaagagaaaa
    10261 attgtgtact tgatgtcctt gggtattttg aagaagatta cggcataaaa attgaaaaat
    10321 acgaaaaaga gggatggtta aaatatcatg gatatcaatc tgatattcgt ccctttgtag
    10381 aaagagcaca ttgctttgtt ttgcctagtt ggcacgaagg aatggcaaat actaaccttg
    10441 aatcagcagc atcagggaga ccaattatta caagtgatat cccagggtgt aaagaagcag
    10501 ttattgaatc gaagtctggt tatttatgtg aaagaaaaaa tagttgtagt ctctataatt
    10561 cgatgaataa atttctaaaa ttatcatatg agcagcgcat taacatggga aaaatgggga
    10621 gaaattatat gattaaaaaa tttaataaga agaaagttgt cgatgaaaca attaaatcat
    10681 tatttagtta aaaagatatg ttttgcaaag tatgtgatag aaaagaggga ttctgtggct
    10741 tcagaataga tatcaaagaa ttggcaaaag tttctgatat ttttttattt actacattac
    10801 aagaaggaat gccaagatct atgatggaag ctatggcaag tggattgcca tgtatagcaa
    10861 gtaaaatccg aggtaatgta gaccttttag tggaaggaaa aggtggatat cttgttccag
    10921 ctaaagatgc agaattagta gcagagtgtt tgagagaact aatcaataat aaatctctta
    10981 gaaagtatat gggaaatgag aacctttcca gaattaaaga attcgaggtt agtatcgtaa
    11041 aaaaaagaat caaagaaata tatgaagaag ttataggtga gagtgtaaaa tgaaaaatcc
    11101 aatgttttca ataatagtac ctatctattg cgttgaaaaa tatttgaaag attgcttaga
    11161 ttcaataaga aatcagacat ttaaaaattt tgaagcaata ctagttgatg acggtagtcc
    11221 tgacgaatgc ccagaaatat gtgactatta ttcccaaata gatggacgtt taaaagtaat
    11281 tcataagaaa aatggtggaa taatagatgc acggaaaata ggagtcacta gtgctatggg
    11341 agagtatata gtttttctag atggtgatga ttggcttgca aatacttatt tagaaaaata
    11401 tgcggaagta attactaagt acaatccaga tataatatgt agtgggtatt ttgcatcacg
    11461 ttccaacaaa attagaaaaa gagaattgtc ttgctcgttt ggatattatg acaaaaataa
    11521 aattataaat ttgatttatc ctaaattaat tgaggatgaa catggagaat attttagtcc
    11581 acaattatgg gcaaaagctt ttagaactac tctgtacaag gagcaacaac aagtatctgg
    11641 gcaagttagt gttggtgaag atcatgcatg tacaaaacca tgtatttacc atgcaaattc
    11701 gatatatatg atggaaaaat gtttatatta ttacagaata aacgaaaatt ctataacaaa
    11761 aaaaccacaa ccatattatt gggaaggacc aaaggctatt ggagagcatt ttgaaaagca
    11821 gattgatatg aataagtatg attttcaaga tcaagtatat agaaatgtta cacacaattt
    11881 atttaatgtt gctgtttcac aatttaataa acaggacaaa tatttaaata taaaaaaaga
    11941 tattatacaa aatctaagtg acgaatacta tactagagct atttgtaaat gcaaatataa
    12001 agataattgg aaaggaatta gtgctaagtt tgcattgaaa aacaaatggg tatttttaat
    12061 ttatatttat aacaaattga taaagaggta ataataatgt cagttaagat tagtgtaatt
    12121 gtaccttgtt ataatgttga aaaatatatt gatgagtgta taaatagttt gataaatcaa
    12181 acattgaaag atattgagat tattttgtta aacgatggtt caacagataa tacgttggcg
    12241 atattaaaga aatatgaaaa gcttgattct agaataaaag tttacgaaca taaaaatatg
    12301 gggttagggc caactagaaa tcgagggatt aaaatagcta atggagaatt tttggctttt
    12361 gtggattctg atgattttgt ttccaatact atgtttgaaa gtttatataa ttgtgcaatt
    12421 gcagagaatg cggacattgc aattggagaa gtaggcattt tttctgatag agaaatagaa
    12481 agagtaaaaa taagaaaaag tctaacgaac ttaaaaacaa ttgaattagc aacatataat
    12541 aagaaagaat tttttaagca attctatttc aatcgtttat attcacataa tgcttgggat
    12601 aagatatata gacgtagttt agtattagag tttaatttat catttggtga taacaaaata
    12661 atttttgcag aagataattg gtttcaatta caagtattta tgacaaatac aagaatctgt
    12721 ttcgtagata aattgtgtta ttattatcgc caacaaaata attctataat gcataagcca
    12781 aaaagtaatt tggtttttag acatggaaat atgattgctg attatgaaaa gttgataagg
    12841 caaaatggaa acaacataat agataaaaaa gcttgtatgt tggttgcatt tgatgtacta
    12901 attatggaga tttttaatca aaaaaacgtt ggtggaacat gtgaagatta taagaatgca
    12961 ttagtttctt tgaaacatca taaaatattg agagaaaata tttcaaatat agttagaatg
    13021 aaagcatatg aacttgaacc taatatgaga aagaagttag cccttgtgac cattagtata
    13081 atgtttaatt ttaaaatgta taaaacagcg tataatatgc tctggagaat atataatcga
    13141 taaagaggta ggaaatatga ggaaattgac ctttgtgata ggaattatgg gaaaaggagg
    13201 ggcagaacga gtaattgcga atttggcaaa taattttatt caagataact gggaaattga
    13261 tattataact atatatggga atcggcaaga ctatgaactg aatagaaaaa tacaggtaca
    13321 tccaataata tgtaaaagtt cgtttcgtgc atttagacct ttggaacgct ttaaggaact
    13381 taggaaaaaa ataagggaaa tacagcctga tctggttatt tcgttccttg ctgatgtaaa
    13441 tatccatgtc attatttcat tgttaggagt aaaacaaaaa ttaattgttt ctgaacgaaa
    13501 tgatccacaa cggaatccag aagaaccatg gattaggaaa attagagatt ttctttataa
    13561 aaatgtagat ggaattgttt ttcaaacacc agacgcaaaa aaatatttta gtaagatcaa
    13621 tgataaaaaa gtaaaaacaa ttataattcc caacccaata aaggaaaatt taccttatta
    13681 taataaaact acaagtagta aaaaatttat tactgcatgt agattggata aacaaaaaaa
    13741 tttgccctta atgattgatg caataggaaa acttattcat gaaggatatg agtgtgaact
    13801 ggatatttat ggagaagggg gattaaaaac cgagcttgaa agttatatca accataatgg
    13861 attaacagaa tttgttcatc taaaagaatt tacaaataaa atacatgaaa agatgcaaaa
    13921 tgcggcagga tttgtaattt cttctgatta tgaaggtata tctaattcta tgttagaagc
    13981 actggctatt ggagtgccag ttatcgctac agattgtcca gtaggtggcg caagaatgta
    14041 tttaggtgat agaaatgaaa taggtcatct tgttgaatgt aataatctag ataagttaac
    14101 agatgcaatg cgtagggtac ttttggatag agaaagtgtg gtaagaaaag cgcaaaaagg
    14161 aattaaacta agaaaagaac tgacatctaa aaatatttca aatatatggt acgaatattt
    14221 aaaccagttc ttttaaccaa aaggaggata ttgtgtgaaa gaaaaaataa atatattgat
    14281 gttttcgtta acgttagtcg cgtttacaat attttgcgct ttttcattaa atcagaacta
    14341 ttggctaaat tggcttggaa tattgattta cttatatgca atctgtacat ggcattggag
    14401 aaaaggagat tcaatatttt cattatacac gatttttttc actctttttt tattatttag
    14461 ttatgggcaa tgcattatgt gggcattcgg tattggaaca gatcgtggaa tcggtacaac
    14521 agtagtagct tatggtacgg ggattattcc tagtgaatca gatatgataa tggtaaaatg
    14581 gtattcttgt atttctatgt ttgtatttca cataggagca ctcttattta cgagaaaaac
    14641 cacactaaga aaagttagtt ggtatgaatc tggagatgct gatgccgata gaaagttttt
    14701 atttaaaata gggtgtatca tttctataat agtagtaccg atagtttttg tttctaaatt
    14761 actagaggta cgtatagcat tagtccatgg ttataatgca ttatattatg gtgattatgc
    14821 tacacaaagt ggatatttac aaattattct atatcttttt tttccagcct taatatgttt
    14881 attaattggt agcaactttt caaaagtaat aacgagattt gtttttatta tttttgcttt
    14941 atattcttta ttaggaatta tgtcaggaga cagaggaagt tggttgtata gctctattat
    15001 actcatatgg gcatacactc aatataaagg aacctttaat tataaaaaat taataaagtg
    15061 gcttatttta gctgttatag gaatatatat tctaaacgta ataacgaaag cacgtgatgg
    15121 tggaggacta agtaaactta ctttaaaaga ttttacatct gtatttgatt cagatgattc
    15181 tcctttagtt gattcttttt ttgaaatggg ggggactatg agtattataa cttttttcct
    15241 aataagtggt aatggaatat atccctttgc taatacttat ttgacctcac tcttaggctc
    15301 tatatcaaca agaatgctat caatgtttgg aataaagttt gttttattag ctgattggtt
    15361 ttctcaggat tatttaggaa tcacttgggg gactggattc tcaatgattg cagaagccta
    15421 tgtcaatggt ggatacattg ggggtatagt ctatattttt attattggta tagtcatcgg
    15481 taaaatcctt tctaatagta tagacaacca agatattgaa aatcatccta tgaagatgtt
    15541 tttggctata actacagcaa atattataat gggttttcct agagctgctt catatttaat
    15601 tgtgaaaaat tttgtgtatg gtgttttaat tatattgttg ataaactttt tcctacagca
    15661 atttaataaa cgtctttaat atttaagctt aataaaaaga taaaacaagt caggagttgc
    15721 aaaaatggaa agaactgctc ggctcaaaaa aaacactatt tgggctctgg tgtttcaaat
    15781 aacttctatt gtctgtggta tgatcttgct acgtttgata ttaacaaagt acggatccga
    15841 ggttaatgga atgattagct ctattaacca atttctacaa attatttctt ttttacaatt
    15901 aggagtaggc gctgtaatgc aatcaactct atacaaacca ctagcagaaa aggataattt
    15961 aatgataagt caaatcttag cttctggagg gaagttttat cggcaaattg catatattct
    16021 actagtatac gtgggtttgt tacttgcagt ctattatgtt ctcgttccta atgaatttgg
    16081 gagatcatat acaatggcaa tgattgcaac attaagtatt aattcatttg ctcaatatta
    16141 ttttggtatt gttaatactt tgttattaag tgctgaccag aaaatttata ttacgtatat
    16201 tgcacagaca atagcattta tttctaatac agttgcatgt gctttattaa ttagtctgaa
    16261 cacagagatc cttatggtaa aacttattac tgctataatt tatcttgctt gtccaatata
    16321 ttatagatgg tatgtggatc gaaactataa tataaagagg aatgtgtcat atgataagga
    16381 accgatccct caaaaatgga atggggttgc acaacacgta gcttctgttg tactagatag
    16441 tactgatgtt gtagtgttat ctatattttc taacctgata tatgtatcta tatataatgt
    16501 gtacaattta gtaatatacg gagtaaaaca gctattcatg gctatgacaa atggcgttca
    16561 ggccttacta ggggaacttt gggccagaaa agaaatagaa gttctttaca aaacatttgc
    16621 atggttcgaa tggattgtac atactttgac agtttttatt tttggctgta ccggtatact
    16681 aatcgttcca tttattcaag tatatacaag aggagtaaca gatgctaatt ataatcaatt
    16741 atcattcgca gctttacttg taactgctaa cgcacttcac tgtctgcgta caccttatca
    16801 tgtaatgata aaagcagctg gtaaatataa agaaactcaa aatagtttta taattgcttc
    16861 tttgttaaat ttaggtttat cattaatttt ggttaaacca ctagggctag ttggagtagc
    16921 attaggatct ctaatttcaa tcacatatca tacactttgg agtgcaaatt ataattcaaa
    16981 atatttgctt aaatggccga tgaaaaaatt ctataaacag ttatttgtaa acatcttaac
    17041 tgtagttctt tcttttgggg cgacatcaca attgaaaatg atgtcaatca catatttgga
    17101 ttggattgtt ctcgcaatta aatgtgcagt tattgtattt atatgtataa tcctagttaa
    17161 tgttttattt tatagaagat atgtagtaaa aataattagt aaaatcatcg taaaaataaa
    17221 gaaaatttaa tacacaataa atgttttaat aaacttaata aaattatatt tgccaaaatt
//
LOCUS       DAPBCS010000060        16615 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPBCS010000060 DAPBCS010000000
VERSION     DAPBCS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.062"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..525
                     /gene="scm"
                     /locus_tag="RD521_002367"
     CDS             <1..525
                     /gene="scm"
                     /locus_tag="RD521_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDT7772051.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
     gene            complement(680..1666)
                     /locus_tag="RD521_002368"
     CDS             complement(680..1666)
                     /locus_tag="RD521_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDT7772052.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1689..2255)
                     /locus_tag="RD521_002369"
     CDS             complement(1689..2255)
                     /locus_tag="RD521_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDT7772053.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2269..2685)
                     /locus_tag="RD521_002370"
     CDS             complement(2269..2685)
                     /locus_tag="RD521_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDT7772054.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3142..5076)
                     /locus_tag="RD521_002371"
     CDS             complement(3142..5076)
                     /locus_tag="RD521_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7772055.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5357..6313
                     /locus_tag="RD521_002372"
     CDS             5357..6313
                     /locus_tag="RD521_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDT7772056.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6403..7506
                     /locus_tag="RD521_002373"
     CDS             6403..7506
                     /locus_tag="RD521_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDT7772057.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7519..8472
                     /gene="menA"
                     /locus_tag="RD521_002374"
     CDS             7519..8472
                     /gene="menA"
                     /locus_tag="RD521_002374"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDT7772058.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9407..10753)
                     /gene="gor"
                     /locus_tag="RD521_002375"
     CDS             complement(9407..10753)
                     /gene="gor"
                     /locus_tag="RD521_002375"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDT7772059.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10887..12140)
                     /locus_tag="RD521_002376"
     CDS             complement(10887..12140)
                     /locus_tag="RD521_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDT7772060.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(12146..13069)
                     /locus_tag="RD521_002377"
     CDS             complement(12146..13069)
                     /locus_tag="RD521_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDT7772061.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13290..15782)
                     /locus_tag="RD521_002378"
     CDS             complement(13290..15782)
                     /locus_tag="RD521_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDT7772062.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15789..16256)
                     /locus_tag="RD521_002379"
     CDS             complement(15789..16256)
                     /locus_tag="RD521_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDT7772063.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(16392..16465)
                     /locus_tag="RD521_002380"
     tRNA            complement(16392..16465)
                     /locus_tag="RD521_002380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16429..16431),aa:Arg,seq:acg)
     gene            complement(16523..16596)
                     /locus_tag="RD521_002381"
     tRNA            complement(16523..16596)
                     /locus_tag="RD521_002381"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16560..16562),aa:Pro,seq:tgg)
BASE COUNT         4908 a         3476 c         2956 g         5275 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcacttttat gagttataag aaatcgaaaa ggtaaaaaaa tattcaagat
      541 aaaaaagagc aacgtcgcta acggacgttg ctctttattt ttgcttttta tcataaatac
      601 aagaactaca attcaatttg tcaaaaaaca cgtagatttc cttctgagaa atttatttac
      661 gtatcgaacc tttccctatt cagtctttgc gatccaaaat cagttgggta agggaaaaga
      721 gattctcttt tgttcgaaga gaggtggcag gtaatttctt tatttccttc aaagcctttt
      781 ctgtgtaaga ggccgctaac ttcctagctt cttccacgcc tcctgtatgt tcaacgattt
      841 tgcgtacggc gtcgacatct tcttctgtca ttgccgcttt cttctctaaa taaggtagta
      901 gctttggttt gtttttttgc aaagagtaga tcaacggtaa agaatagacc ccttgacgca
      961 tgtcttctag tacaggtttg ccgatttcct ggctctcttt tgtataatct aaaatatcat
     1021 cgataatctg aaaggccatc ccgatagaaa gtccgatatc tcctgctttt tttgcgaagc
     1081 gctcgcttgt tccactttca aaagcgccaa cagaacaact taattggaac agttcagctg
     1141 tcttacctga aatattttcc aaatattctt ttacagaaag ttcataatta tagcgatcat
     1201 ccatctgacc taattcgcca ttcaatactt tttccatact gcgagaattc aattggagac
     1261 ttttcaagga agtggagtaa tcagacaata gtttaaaaca gcagacaaat aaatagtctc
     1321 ctgcatagac agaaacgaca ttaccaaact gactacggat attaggctga ccacgtcgca
     1381 gatccgcttc atcaacgata tcatcatgga tcagtgtggc cgtatggagc atttcgatag
     1441 aagctgccaa agcgatggct ttgtctcgat cttgctctgg tccaaattgt gagaacaata
     1501 gctgataggc aggacgcaac agctttccgc cggcatggat cgtttctttg attgcttgtt
     1561 cgacaggttt gtttttcagg cggacagttt cttccatccg ttttagtgtt tctgtcaatt
     1621 ctttacttaa agcaggatag ttttcccaca ttggatgtag cttcataaaa aaactccttg
     1681 tgtgacgatt attttttggt aaacattggg ctccattttt gtttggttcc ctgctcataa
     1741 tctttttgga atcgttgaag tgtctgcaca tgtcgtaaaa gataattcgc tgcgatcccg
     1801 attgcaatcc ctgataaaat ccctaaaact gaaaggactg gcaagtacaa catgactgtc
     1861 catgattgtg caatgaaaga agccatcaaa agctgtccga cattatgcat gaaaccgccg
     1921 gccgcactaa ttccgatgat actgacacgt tttggtccta attgcttgac caataacatt
     1981 ccaaaataac taagcaaagc accactcata ctataaagga atgtggaaat cgttccgcct
     2041 agcaaagtag tcaggaatag tcgtagccaa agtagtaaga agctgtctcg ttttttcatg
     2101 gtaaaaatgg caacgatcgt gattagattg gctaagccta gttttgcacc aggcgcaaaa
     2161 gaaaaaggat aagggatcat attttccaat aaaccaatga tcactccttg tgcaacaagt
     2221 agcgaaatat aaatcatttt ttgtaattta ctcatggtgt gtgaaggtct aataaatcag
     2281 actgtcttcg tcacctccat ctgttgaacg gacttcgatc actaatttat gtggcaaaca
     2341 gacgatggtt tcgccatttt tcgttgccca gcctcgacgt acgcagatct ggtcatcgca
     2401 atctgcttct tttatccgta ctcgatcgcc atctatctcg atcaagttat aatctccgtg
     2461 ttcatctttg taagtatatg tgttgctctc ttctccagcc atcaaaggga atgttttgat
     2521 ttctgtgcca tccacacgca gtaccgcttc tttttctgct gttgcggtgt tttgctgaag
     2581 accaaaaatc atgactggta aaaaagaaag aacagtcaaa agcaaaataa taaaaacatc
     2641 ccaaggtttg agcagacttt tttcagcaaa ttctttaagg ctcatttttg ttctcctttt
     2701 tccagattaa ctcacccaat tttagcataa aaacaagagg ttgtgacacc tgtgtcttgc
     2761 tttgagtaat aagaacgaag aatagaaaac agctttccat gttttctaat tctttgggct
     2821 tattaccgag aagcagcagg tggaacaccg tttataacaa gaggttgtga cagaagtggg
     2881 caacttcaag aaataagaag gaatttacga aaattacttt tcaagttttt gtgaaatttc
     2941 ggcttatttt tcgaagaagc tacttctgga acaccgttta taacaagagg ttgtaacaga
     3001 ggttgtgaca cctgtgtctt gctcatggag gcttatctcg ggcatcttat tcctttaatt
     3061 ttacataaaa aggaggctgt gacggtttgt ctcagcccct ttctattgaa atcttgcatt
     3121 caaataacgt tctttatcta tttatttctt gccgtactgg gaacttggtc taccgtacca
     3181 ccaattacct agtgtacgtt gtagccatgg aattacccag cgatccaacc caatcgcacg
     3241 tccggaaccg ttcatcaatg cgaaagcaac aaaaatgaac caaatgttta cccagtaaaa
     3301 catgcctgat aagcagaacg tgatcaccaa tacaatcgtt gtcgcactgc atagccatgt
     3361 gaataatcca gcaatcaatg ctaatgccaa ggcaatttca aagaatacca tgaatttttg
     3421 gaagaacatt gctacatcat tgtttggcat gatcacttgc atgatctttg tgaaccagtc
     3481 tggtgacttc tcaatcacag ccattggttc ttcaccgtat gcataactca aaccaaaatg
     3541 tgcagcttga gtcgctgctt ctccggcact tgcccctgct ccgctggcag ctgtggttgc
     3601 atcaccagca gcagaagcac cactggcaac atctgttaca ttgtattccc atgtccaagg
     3661 gaacacactg tttccagtga accatgatcc ttcaccaaac caagtacttg gtttgaacac
     3721 ttcaccattc ccaatgattt ttttggaagc ttcaacgaac caaactaaac catagaaaat
     3781 acgaagtggc acactccata agacattgcc gtaacgagaa gaatgtcctc gagtcacgtt
     3841 gcgatcatct ttgatatgga aaaactcatg cattaaatat tggaacatat aataaccaga
     3901 acgaatatca aagaagtatt tcaaattcac gatatgtttc attacgatag ctaaaaatcc
     3961 gcttaaatgg atcttatcaa ataggttagc taccccccat ttggcaccaa tagaaaccat
     4021 aaatccttga tagtttcctt taaatttatg cttttctccg cctttgatcg aagccacgat
     4081 attcgctgcg gctgtgtgtc ctgtttgttc agcagcttga acgatctgcg gtgtcggtgt
     4141 tcctgggaat tcttcataaa agactaaatc accaatgata tagatgtttt tatcttcgta
     4201 gccttttgcc tgcatatatt cattggcgat caaccgctgt ccgcgtgctg attctaatcc
     4261 aaagtcagca gcgtcagaag ttcctttgac accagctgtc caaatcagtg tgtgagtcgg
     4321 aatgttgcgt ccgtctttca atttgatatg atcagcagcc acttctacga ttggtgcatt
     4381 caataataat tccacgttgt gcttacgcaa atagcgttca gcttttgcag catcatttcg
     4441 atccaacata ttcaagatcg tcggcattgc ctctacgacc atcagtgtga tttcgtttgg
     4501 atctaattta taatctttgg ctaggcgatt tttccagtcg atcagttccc caatcatttc
     4561 aataccggta aatccagaac cacagacaac gaaagtcagc atggctttac gcagttcatc
     4621 atctggttcc aatgcagctt ttgctactgt cttctcaata tgttcccgaa tccttacaga
     4681 atcttcaaat gaccaaaggg tgaatccgtg tgctttgaca ccaggtgtac caaagtcatt
     4741 cggctctccg cccattccaa ggatcagctg atcaaaagga taggaaccta atttggtctt
     4801 gacgattttt cgttctttat cgataccgat tacagtgtct gtgacaagtt gtacattctt
     4861 ttgacgagca aacaaatgct gcagatcgta ttggattgct tctggttcta cacgtccgcc
     4921 agctacttca tgcaactcgg tcatcatagt atggtacgaa tgacgatcga tcagagtaat
     4981 tgctacatct tcttttttca gtttctttgc caagtatttt gtagctgaaa caccggcata
     5041 tcctgctcct acaacgacaa tattttgctt tgccattgat catccgctcc ttatagacta
     5101 tctatataat gaatatatga taattctttt agtttgatac ttattacaca atctccatat
     5161 attatataga tgttctgttc ttttgtctaa ctccttttgg atttttttca aattttaaaa
     5221 taaagcgttt tttcacgaaa tttgctgaaa gaaaacgttt gaaatattac atgggttatt
     5281 tatcaaaaat agttgactta taggctaaaa agattagaat gtataatgaa tcacaaagaa
     5341 aaattgtgga ctagaaatgg aatggtgggg tattaaaatg aagaaaaaac gttttgtaac
     5401 tggatttgcc gtattagcat tttcagctct agtgttaggc gcttgcggcg cagatgacaa
     5461 cggaagctca aactcttctt ctgaatcatc tacagcacaa tcttcgactg caaaaagcac
     5521 aacagaatca tctgcaaaag ttgtagctgg cggagatttg caagacggca catataaatt
     5581 agaagaaaaa aattattcaa atggttaccg tgcagtcttt gaaatggttg taaaagacgg
     5641 caaaatcact gaatcaaaat atgataacgt caatgaaaac ggcgaatcaa aaacaaaaga
     5701 tgcagaatac aacaaaaaca tggaagcaaa atctggcaca aacccagaaa aatacattcc
     5761 tgagttgaat gaccaattct taaaagcaca aagcgcaagc ggtgtggaag tcgtaactgg
     5821 agctactcac tcatctgaaa gcttccaaaa ctacgcacaa caattgatcc aagctgctca
     5881 agctggtaac acagatacaa tcgaaatcga caatggtgct gatttgaaag acggcactta
     5941 caagcttgaa gaaaagaact actctaacgg ctaccgtgtg caattcgaaa tgacagttgc
     6001 tggcggcaaa gtaactgaat caaactttga ttatatcgac aaagacggta aatcaaaaca
     6061 agatgataca gaatataatg aaaatatgaa agcaaaaagc ggtacagaac caaaaactta
     6121 catccctact ctgaacgacg aattcgtcaa agcaatgggc gaagaagatg gctcacctgc
     6181 taatgtagaa gttgttacag gtgctacaca cagctcacat tcattcatca tgtacgcaca
     6241 acaattagtg aatgcagctg aaaaaggcga cactcaaaca atcgaagtag ataatatcgt
     6301 aacagaaaaa taatcacata atcatactaa taaaagttgg agggtgggac gatagccaga
     6361 ttaggcagtc gtctcacctt tcaaaatgaa gagaggaaaa agatgcggaa aaaactacta
     6421 ttttccttaa tcacactggg catctctttt atggtgctaa ctggctgcag caactcagca
     6481 aataaaacaa ccactgaaag ttcagcagct accaagatat tgaaagatcc atattctgat
     6541 gaacagttct tgttagggac ttacgttcgt atccgtgtgt atgatgaagg caaagaatca
     6601 gcattgaaac ctgccttcga tcgagtaaaa gaacttggtg ataaaatcac gatcaatcaa
     6661 aaaggttctg agatcgatga agtgaatgag caagctggga tcaaaccagt aaaagtttct
     6721 gatgacgtct acacgttagt caagcgggcc tacgaatata gccaagattc tcaaggcgga
     6781 ttcgatatgg cgattggtgc aatcacacaa ttatggcgaa tcggttttga tgacgccaga
     6841 aaaccttccc aagaagaaat cgatcaggca ttgaaattag tggattatca taagatcgaa
     6901 ctaaatgaca aagaaaaaac tgtttatcta aaagaaaaag gaatgatcat cgatttaggt
     6961 gcaatcgcca aaggttacat tacggacgaa gtagtgaaag tcttgaaaaa acaaggagtg
     7021 accactgcca tcgttgactt gggcggtaat gtctatgtct taggccacag cccaagaggc
     7081 gaaaaccaag actggacagt cggcatccaa gatccgaaca tggctcgcgg atctgtttta
     7141 ggttctatta aagaacggaa caaaacatta gttacctctg gaatttacga gcgttattta
     7201 gaagtagatg gcaaaaaata ccatcatttg tttgatccaa aaacgggtta tccgttcgac
     7261 aatgatatcg caagtgtcac gatcataaca gataaatcaa ttgatggtga cgggttgtct
     7321 acagcagtct tttcaatggg tgtgaaaaaa ggactggaat acgtcgaaag cgaattgaat
     7381 aacgggacgg aagcgatctt cgtgacaaaa gacgataaag tatatgtgac cgatccaatc
     7441 aaagacacct tcaaattatc ggaggactcc cattacacga tgggtgaccg ttctgaacta
     7501 aaataaggag tacgaaccat gacactaaaa atctttttag aagtagttga aatacgaaca
     7561 aaggtggcta gtgtatttcc ttttgcggta ggggtattat tctctatcgc tttttttcat
     7621 gagatacatt ggctaaacac ttttttattt tttattggaa tgctagtttt tgatttagcg
     7681 actaccgcta tcaataatta catggattat gaaaaggctc attctgaagt atataagtat
     7741 gaagaaaatg tcattggccg cagaaatcta tctcctgtat tgattcgaaa catgatttta
     7801 gctatgattg catttgtttt tgttatcggc gttattctaa cgatcaatac gggttggctt
     7861 ttacttttga tgggatttat ctgttgtttt attggagtat tctatacatt cggaccgatt
     7921 cctttatctc gaatgccttt aggggaaatt ttcagtgggg tgactatggg ccttggaatt
     7981 tttgccatgg tcgtttatgt caatacattc ggttcagatg tctatgatct gacagtcaac
     8041 ttttcatctg gacaatttgc tttggtagga aatctttggg ctatagcagc aatcgtatta
     8101 gcttccttac ccttggtgtt tacgattgcc aatatcatgt tggctaataa tttgcgggat
     8161 cttgaccgtg atattgaaaa tcatcgctat acactagttt tttatatcgg acgtcctgcg
     8221 ggaacagtct tattccagct attgatgtat gcttgctatt tcgtgatttt aatggggttg
     8281 ttgagcggta tctatgaatg gccgatcttg gtcaccttct tgaccttgcc aattgtgaag
     8341 aagcatttgg atcaattcaa gcaaacgtta ccccagccta tcagtttcag ctattccatc
     8401 aaaaatatgg tcttattcaa tgccagttat gtattggggc tattactaac aatcattatg
     8461 caagccattt aacagcatga agaggttgtg aaaaaactgc cctgcatcaa gtaataagaa
     8521 cagccaaata aaaatagctc cttatatttt tcgtttggtt gggcttatta ccgcagatgc
     8581 aagcttttga acaccgttta tcaggaggtt gtgaaaaagt cgcttagttt cgagtattaa
     8641 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
     8701 actggctttt gaacaccgtt tatcagaggt tgtgaaaaag tcgcttagtt tcgagtatta
     8761 agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt aataccgaga
     8821 aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt cctgagtatt
     8881 agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct aataccgaag
     8941 gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac aatttttttc
     9001 acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga actttcttgg
     9061 tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat ttatttatac
     9121 aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt taaacatcga
     9181 taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct tcgtcagtta
     9241 cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt atcgaagcaa
     9301 tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat tttctggttt
     9361 tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat cgcatcgtta
     9421 cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct tctttagttg
     9481 cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca acaccaatag
     9541 catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt tcgcattttt
     9601 ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa actttcaatt
     9661 cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt gcaattggtg
     9721 gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct ttctttccat
     9781 taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc agatctaatt
     9841 taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg tatttatcta
     9901 ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct aatttttcgg
     9961 tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt tcaccatttt
    10021 caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata tgatgtgcgt
    10081 aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc attttatcaa
    10141 acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct aaaccacaaa
    10201 atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct ctcttaggta
    10261 actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca ggaatggtca
    10321 ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag gtttcaccgt
    10381 ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc tcgacatgat
    10441 tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt tctcgtcttt
    10501 ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa cttgaggcat
    10561 ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta ggtacacaac
    10621 cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact tttgcgccat
    10681 gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt acgatgtaat
    10741 catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt tatttaacga
    10801 tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc gaagatgctt
    10861 aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc tccaggtttc
    10921 accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg gtcaatggat
    10981 tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa tcctggacca
    11041 taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt agcaatctgt
    11101 gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat tttacgacgt
    11161 tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt agctaattct
    11221 cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata gttttcagga
    11281 tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga tacataatgg
    11341 atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat caattctgga
    11401 atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc cacttcgcct
    11461 gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc atacttccag
    11521 cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg gttggacaat
    11581 gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc gatttcggta
    11641 tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc taaaacgaca
    11701 cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc tgaagcttga
    11761 gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat caaagctgga
    11821 tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa ttcccctgct
    11881 ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt cgcatcatgt
    11941 tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc tgcacggctt
    12001 cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg gatcgcggtt
    12061 tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa ttttcttttt
    12121 actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata taaaagaatc
    12181 ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc attttttccg
    12241 ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg aatagtcgag
    12301 tgatgtttac gaatttttcg cctggagtaa ataatccgtc caatttccat gtacagtaat
    12361 gatgctgata aagttcattc aattcattcg agagattcag atcattgctg gcaaaaatat
    12421 gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta ggttccgaga
    12481 taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta tagtaattct
    12541 gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg gtttctacag
    12601 gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca cgagcaagta
    12661 ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc gttactaatg
    12721 tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc gtataaataa
    12781 tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa gccaaatatt
    12841 caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata ttcgcttttt
    12901 taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta aatgaagcag
    12961 gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct gcagcaagca
    13021 actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata attttctcct
    13081 cctacaactt cataatagta gcatagctct aaaaactttc caatacttct tctatttaaa
    13141 gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat attctgtcca
    13201 aatataaaaa gagattgtga caaaaatctt gtcacaacct cttaatcaag tcttactatt
    13261 ataaaaattc agctacacag gccaagtaac tatagggaac ctactgtttc ctttttaggt
    13321 ccgcgaacat tcaatgtgat cttacctttt gaagcaccta ctgttacgtg gtcacctaaa
    13381 cgaatttctc cgccaagcaa agcctcgctc aatcgatctt caatttcttt ttgaagtgcg
    13441 cgacggattg gacgtgcacc atattcagga tcaaatccag ctttcccgat cacatcgatt
    13501 gcagatggtg tgattttcac ttgaatatct tgttctttca atcgtttgat aatcgctttg
    13561 ctcatgattt ggacgattgt atggatttca tctttactca atgaatggaa gacgacagtc
    13621 tcatcgattc gattcaagaa ttcaggtcgg aaagctttct ttaactcttc taagatacgt
    13681 ttttgcatgg ctttgtgatc tttagtaaca tcttggacat tgaatccgac atttttttct
    13741 tctcgaattt gtgtcgctcc gatatttgaa gtcataatca tgattgtatt tctaaagtcc
    13801 acttttctac ctttagaatc tgttaaatga ccatcatcca atacttgcag aagtaaattg
    13861 aaaacgtctg gatgtgcttt ctccacttca tctaagagaa tcactgaata tggtttttga
    13921 cgaatttttt ctgtcaactg tccaccttca tcatacccta cgtatcctgg aggagacccg
    13981 attaatcggc ttgtactgta tttttccata aattctgaca tatcaacacg gatcagggca
    14041 tcttcacttc caaacatgac ttctgaaagt gctttggcca attctgtttt tccgacacca
    14101 gttggtccaa ggaacatgaa tgaaccaatt ggacgatcag gatctttcaa tccgcttcgt
    14161 gcacggcgga tcgcacgtga aactgctttg acagcctcat cttgaccaac gacacgttca
    14221 tgcagaagtc cttccagttc taacaagcgt tcgctttctt ttttctccaa ctgctgtaga
    14281 gggacaccag tccactccga tacaactgta gcaacatctt cttctgttac acgatcagaa
    14341 taaccttgct cctgttttgc ttcaattgca gaaacttttt gcagcttttt acgtagtgct
    14401 ttttcttgtc tgcgtaattg agcagcattt tcaaagtctt gtttttggat cgctgcttcc
    14461 ttttcttgta ctagttcatc gatttctagt ttgatcacag ttgattttgt taagtgatcg
    14521 gtttgatcca aacggacttt cgctgctgat tcatcgatca aatcaattgc tttatcgggt
    14581 agttgacgag aagtgatata acgaacagaa agattgactg ctgctcgtac agcttcatct
    14641 gtgatctcca ctccatggtg ctcttcatat cttgtacgta atccttgaag aatcacttct
    14701 gcttcttcag gagttggctc atctacttga atgcgagcaa aacgtcgctc taatgcagaa
    14761 tctttttcga tatatttttg atattcatct agggtcgttg ctccgatcgt ttgtaattct
    14821 cctcgagcta acgccggttt caagatattc gaagcatcaa ttgctccttc tgctccacct
    14881 gcaccaatta aggtatgcaa ttcatcaatg aataagatta cttgaccatc attatagatt
    14941 tcgtcgatga cctttttcat tcgatcttca aattcaccgc gatatttcgt accagcaacc
    15001 aaagatccca tatcaagcat catcaggcgc ttttcttgca tgtcttctgg cacttcacct
    15061 aaaatgattt tttgagctaa cccttcagca atggctgttt tgcctacacc aggttcaccc
    15121 accagtacag ggttattctt tgtccgtcgg ctaagaatct ggataagccg tttcacttct
    15181 ctcgaacgac caacaactgg gtccagcttg ttttcttttg ctaatttcgt caaatcacgt
    15241 gctaatgaat ctagcgtagg tgttccttgc ggtgtttgcc gtttatcttg tacatttaca
    15301 tttctacgac gattcattcc ttttccagca gaatcaccag ctagccccat tttttgtttc
    15361 agaacttgac gggcttttga aaggctgata cctaagttga tcatgattcg agtagccatg
    15421 atactttcat cttttaataa gctcatcaaa atatgttctg ttccaacttt ttgagaacca
    15481 gttttcttca cttcgctgct agctaaagca aaaacttgtt tcattcttgg tgaatatgga
    15541 agaaagactc ctttagggta agaacggata ccaccataac caatcagatg ttcaatttct
    15601 tcataaattt ccgtttcatt caaatccatt tcacgtaata cttttccagc aattccattt
    15661 tgctcgacca ctaaggctag taaaacatgt tctgaactaa ctgattgatg tttcaaccgt
    15721 tttgcttctt cttgtgcaat ttctaatact gcttgggctc tttcagtaaa aagttcttcc
    15781 atctaatctc acttcccatc ttcataactt aaacgttcca gaaatgctcg gagaatccga
    15841 gcacgtgtat gatcttcata ttcattgaaa ttcaatgtgt tttttgcaat tgcactcaac
    15901 atcaagtttc cttctttttt tgtaattatt tggtcttcat aaagtttttg aataatagca
    15961 aaagcatttt tctcactgat tgtatcatca aataactgat ttatctgctc aagtaattgt
    16021 tttttatctg aaatccttac ttttgcaatt cgtatataac caccaccacc acgtttgctt
    16081 tctactagat aaccacgttg gatggtaaaa cgggtattaa tcacatagtt gatttgtgaa
    16141 ggaacacagt taaagaggtc tgccatttca atccttcgaa tctcgatcat ttcactattt
    16201 tccagaattt tctttatata tgattcaatt aaatccgagg tattctgatg tgccatctta
    16261 ttcattccct ttgactaata ttgaccttaa ttataacgaa tttgataata gaaagaaaga
    16321 agaacgcttt ttgttaaata aatcctttag aatcaacaaa aaaaaatacg gaatatacta
    16381 ttccgtatcg acgcgcccaa gaggagtcga acccctaacc ttttgatccg tagtcaaaca
    16441 ctctatccag ttgagctatg ggcgcatatt caatttaaaa aaattataaa tcatttgaaa
    16501 acaaaaagca agataaaaaa aatcgggaag acaggattcg aacctgcgac cccttggtcc
    16561 caaaccaagt gctctaccaa gctgagctac ttcccgttta aaaaataaaa aagac
//
LOCUS       DAPBCS010000061        16593 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPBCS010000061 DAPBCS010000000
VERSION     DAPBCS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.063"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(82..789)
                     /locus_tag="RD521_002382"
     CDS             complement(82..789)
                     /locus_tag="RD521_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDT7772064.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(868..1206)
                     /locus_tag="RD521_002383"
     CDS             complement(868..1206)
                     /locus_tag="RD521_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDT7772065.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1317..2279)
                     /locus_tag="RD521_002384"
     CDS             complement(1317..2279)
                     /locus_tag="RD521_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDT7772066.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2294..3625)
                     /locus_tag="RD521_002385"
     CDS             complement(2294..3625)
                     /locus_tag="RD521_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDT7772067.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3633..4313)
                     /locus_tag="RD521_002386"
     CDS             complement(3633..4313)
                     /locus_tag="RD521_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDT7772068.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4532..5011)
                     /locus_tag="RD521_002387"
     CDS             complement(4532..5011)
                     /locus_tag="RD521_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDT7772069.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5138..6817)
                     /locus_tag="RD521_002388"
     CDS             complement(5138..6817)
                     /locus_tag="RD521_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDT7772070.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7365..8867
                     /locus_tag="RD521_002389"
     CDS             7365..8867
                     /locus_tag="RD521_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7772071.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8880..9542
                     /locus_tag="RD521_002390"
     CDS             8880..9542
                     /locus_tag="RD521_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7772072.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9643..9846)
                     /locus_tag="RD521_002391"
     CDS             complement(9643..9846)
                     /locus_tag="RD521_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDT7772073.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9957..11141)
                     /gene="iadA"
                     /locus_tag="RD521_002392"
     CDS             complement(9957..11141)
                     /gene="iadA"
                     /locus_tag="RD521_002392"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDT7772074.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11298..13037)
                     /gene="yfcC"
                     /locus_tag="RD521_002393"
     CDS             complement(11298..13037)
                     /gene="yfcC"
                     /locus_tag="RD521_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDT7772075.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13810..14424)
                     /locus_tag="RD521_002394"
     CDS             complement(13810..14424)
                     /locus_tag="RD521_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7772076.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15030..15407)
                     /locus_tag="RD521_002395"
     CDS             complement(15030..15407)
                     /locus_tag="RD521_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772077.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15707..16588)
                     /gene="galU"
                     /locus_tag="RD521_002396"
     CDS             complement(15707..16588)
                     /gene="galU"
                     /locus_tag="RD521_002396"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDT7772078.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4809 a         3574 c         2832 g         5378 t
ORIGIN      
        1 ttattccaaa taaatggtat tcaatcagct aactctcggt attagctcag atcaaagcgc
       61 tgatttcaca acttctttat tttaatctct tcggttgccg ccgaacaata agatcaagcg
      121 caacagctgc aggaatgtcg aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa
      181 tacgtgtctt gccatcggta cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg
      241 gattgctcgc cgagacgcgt taaattcgac tggtaatgtc accaattgga aaagtaatgc
      301 taaagaaaaa gcgaaaatcc cgatattgat taacgtagag ttccagctaa gcaatacacc
      361 taccataatc aaggggaacg aaatcatcga accaaagtta gctgctggaa caagtgctgc
      421 acgaatcttc aatgggacat aacccgaagc atcttgtacc gcatgtccgc attcatgagc
      481 tgctacacct atcgcagcaa ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat
      541 cttattacct gaattgtagt tatctgtgag atctccggca atttgttgaa caccgacatt
      601 atttatctgt tgactttgta atatatattg tgctgcttgt gtaccagtca cattgttttt
      661 gcttctcact tgatcgtatt ttcggaaagt actattgacg tacgctgacg caattccgga
      721 aataactaat ccaagaatga ccaaaagaaa tgtcggatct aaaaaaccat acattccata
      781 accatacatt ttcgtttcac tccttttcgt ttactttatt ttatcattag tagtaggaaa
      841 agaatgtgaa aagaccttga atattcttta attctttttc acaaattccg atttcagttt
      901 catcggtcca aatccttcta ccttgcaatc gatattatga tcgccttcta ctaaacggat
      961 gttcttgact tttgttcctt gtttaatggc acctgacgca ccttttactt tcaagtcttt
     1021 aatcactgtc acgctatcgc cctctaacag acggtttcca tttgcatcta ataccactaa
     1081 tccgtcttct atgacagttt cttgttcttc tttgttccat tcgttcccgc attctgggca
     1141 aatcaacaag cctcgatctt catatgtgta ctctgagcca cattctgggc aatttggtaa
     1201 tgtcatcttc tttctccttt aaaatcttta gtgagcacta ttgtaacgga aaaagaagat
     1261 cttgaaaaga tacatttgtg atttttacta caaaaggcac gttttcgaac gaagtatcat
     1321 tctgtttttc tgctcttata cgattggcat atatcttgga tctctttata gatttcctct
     1381 acttctgcta aatcttttgc attcgcacca cttgctaatg gatggcctcc tccatgatgg
     1441 cgtttagcca attcattgat gatcggcccc ttagaacgta aacgtacgcg gtaataccct
     1501 gaaggctgct gaacaaaaat cccccaagcc agcacttcgt caatcactcc aggaagtgaa
     1561 acgatcgaag ctgtctctga atcatcgatt ccatatttat caagaatttc ttgcggaagg
     1621 attattcttc ctgcaccatt ttcatccacc tggatatttt gataaagata accggaaagt
     1681 ttagcgactt tcatcggcat ttgttcaatc tctctgttca aagcacttgc atcaaatgga
     1741 taatttctca atatcgaagc aatctctaat gtacgggacg tcgtggaggg atacaaaaag
     1801 cgccctgtat ctccgacgat tcctgcataa agtaaccgtg cagccttaac gttcatcttt
     1861 aaagattcag gaaacatcaa agcaaattct gcaatcatct cactacagct acttgctttt
     1921 gtatttaccc acacaagatc tccataaggg tcatcattgg gatgatggtc gattttgatc
     1981 agtttttctg ccaactgata tctttgatca ctgattctgg gtgaattagc tgtatcagtc
     2041 acgatgacta gcgctccttg gtagaagaca tcttcaactt cttgcatttc tgctaaaaag
     2101 cgaagccctt caatcgtttc acctacttga cgtatttctt tttctgggaa actttcccgt
     2161 aatagttcgg ctaatcctac ttgtgatcct aatgcatcag gatcagggcg caaatgcctg
     2221 tggataatga ttttatcgta tttttttatt gtatctacta tttcttgctg tactgtcatt
     2281 tcactcctgc tttctaagaa cgttccatta cttgacaaac aacgattgct ttggaaacga
     2341 tggtgttttc aataaacact tcgatatcca gtttagcgga acgtctgccg atttccaata
     2401 cacgtggacg aatgtctaat tcgctttcca attggatcag ccgcaaataa tgtaggttca
     2461 cttgttcaat caagatattt cgtttttggt tcatcaccat tgttttttgt gcgacatcag
     2521 aaatgatttc gctaagtacg ccaaaagaaa tggttccaac gccattgacc atttgtggtg
     2581 ctactacgaa tttgaactta gggttttcca aacgatttcc ttcacggtca gtatcaatcg
     2641 tatggatatt tccagatatc tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga
     2701 caagctgcat cgctttcatt acatcttgcc gggtaatcaa gccttccaat gtcaagtcat
     2761 cggaaacgac cggcatgact tctagcccat cccagatcat ttgatggcta acagaggcaa
     2821 tactcattgt tttctttaca ctgattggtt ctttcgtcat cacccgatcg atgatctggt
     2881 ttggcgattt ccctaaaaca tcttttgccg taacaatccc catcaaacga tgatggcggt
     2941 tgacgactgg aaaacgcgaa tgctgcgttt cttctgacaa acgctgataa tcagaaatcg
     3001 tatctgtctg ctgcaaataa tgagtctttt cagctggcat atagatatca ctgacaagca
     3061 tgatatcttt tttgatcaat tggtcgctta acgctcggtt gatcatggtt gcgactgtaa
     3121 atgtatcata agaggtgcgt aagatcggca tttcaagatc atcagctagc tttgcaatct
     3181 cagcagtcgt attgaatcct cctgtgatca atactgcggc gccatcttcc aatgctaatt
     3241 tttggacttc cgcccggttg ccgacgatca ttagtgatcc tggcgtaata tagcgttcca
     3301 tggcatgttt agtcatcgcg ccgatgacga atttattcaa gaccttgtct aatcctgccg
     3361 ccccgcctaa aacgtcccct tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa
     3421 tatgtttttt taatttgcgt tcgatacgga tggtccctac tcgttgaatt gttgaaacta
     3481 gtccgacatt ttctgcatct ttgattgcac ggtacgctgt tccttcactg acattcaatt
     3541 cttttgcgat gcttcttaca gaaattcgat tgccaacagg taaactttca atatattcta
     3601 aaatcaaatc atgttttgtc gccattttct gcctacactt ccagcgtttc cgagacgctc
     3661 attatttttc cttcactatc ttccagtaac tctacaaaag attcgggatc ttgttcaatg
     3721 acagggaaag tattgtaatg gattggaact actttcttgg cacgaattgt ttgcgcagcc
     3781 tttgctgcat cttctggccc catcgtatag ttatcgccaa tcggcaagaa agcgacatca
     3841 atatcgaatt cttctgcaag caatcctaga tcactgaaat aagcagtatc ccctgcatga
     3901 taaatcgttt ttccttctgc ttgaataata attccgctag gctcgcccat atactgaacg
     3961 ataccgtcta cttcatagct tgaactatgc tgtgcatgta cgaattttac cacgccgaat
     4021 ggaaacgcat gttttccacc gatattcata ccgaatgaat ctactcctct gctgttcgca
     4081 taatcagcaa tctcaacaat actgataatt gtggcttgat tttcttttgc aataggaatc
     4141 atatctccta tgtggtcact atgaccatga gtaatcagca accaatctgt ctttacttct
     4201 tctgcagtta agtctgtcaa tggatttcct gtaataaacg ggtcaatcat cacgcgttgg
     4261 ccattttcca tttcaattgt taaacaggaa tgtccatgat acgtaatttt catattttac
     4321 acctcgcgtt ttctctgtat cttttttatt aaaaacgatt tcatcacttg cacatcaatg
     4381 aaaaaatata gctgctaaat atattttcat tggattgata gtacagtatg tacaaagcaa
     4441 ttgtaacaga taatacagga tgcaccaagt accaagcaga tataatctca agtttgaggt
     4501 ctctgctctg cttagcttct cttctcgtcc cttaactaat taaatcagcg taaataggat
     4561 ggacgatctt ttctaaatcg gctactttta tccgaaggct tctcccgatt tcaccgcttg
     4621 acacaattac ttcttctttt tcttttaaag aactgtctgc taaaaaacaa taatcaggat
     4681 gtgcgagttt tattccaata ggggtgtttg ctccgtgggg atatcctgta tgcattaata
     4741 caaaatccat tccaggaaaa ccaactttac gatctttcgt cacttgtctc gtttttttgt
     4801 aatctaagtg atcgttcaaa ggaatcaatg cgatgatcac cccagttttg tttcctttca
     4861 ctacgagagt tttacatatc aaagctggat caatccctct tttttgtagt tcttctgtga
     4921 aagattcttc taccacatga ctaaaatcaa gtggttgata gctgatcttt tgttcttcta
     4981 aataacgttc gacttttgtt tgactcatca ttgattcact ccctttattc tatcgtttat
     5041 tataaagcga gggatgctgt attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac
     5101 gagcactcta tagagaggtt catcttactc tttttgttta ttttttattt cgacgttgag
     5161 tagcttccat aatgactggc aagatcactg ggcggcgttt tgtttgttca aataagtaac
     5221 ggcctaattg atctcggatg tcttgcttta atttgctcca ctcaaaatca ttgctttcta
     5281 aatgtttctc aacgattgtt tcaatcatat tgctgctttc tttcatcaga tcacggctag
     5341 cttttacgta aacgaatccc cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc
     5401 gccgactgat agtgacaacc gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac
     5461 gtaaaacaat gtttccgata tcacctatgc ctaatccatc aatcattaca ttttctgcac
     5521 ttgtagttcc tgcaacactc atacgcttgt tcttatattc cagcacgtct ccgcgtccag
     5581 taatatagat atttttgtat ggcatcccta ctgcatgagc caaatctgca tgagcagcta
     5641 attcacgata ctctccttga actggcacaa aatatgttgg tttcatcaaa ttgatcatta
     5701 attgcaagtc attaggattg gcatggccag aaacgcgcat attctcagag atcaacttca
     5761 ctgttccacc agctcgataa atgatatctt ccgttttcgc cacgatggtt tccatcgcga
     5821 tgcttggcgt agtagtgata taaaccaaat ctccttcttc aatcttgaca ttacggtgtg
     5881 taccgtttgc cattttttgc agagacttga tgggttcacc cattcggcca gtttctaaaa
     5941 tgatcagttc ttctggatca tagtttttca tgtctttcaa cgtaacaagc agatcttcac
     6001 ttggtaattg aagtttttct aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac
     6061 cagtcaagac aacttttcgt tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt
     6121 gcaaattact tgctacacaa gcgacgatga tacgcccttc ccaatatttg atcgtatcaa
     6181 atacttcatc tgctatttgg gcttcagaag ctacaggctg aggattttct gcatttgccg
     6241 attcacttag taaggctaag actccttctt tgccgatttc cgccaatcgt gcataatctg
     6301 tttgatacat tgggatagcc gtttggtcaa atttaaaatc tcccgtataa acgatactac
     6361 cttcttttgt tttcagattg atccccactg aatctggtat cgtatgagtg gtacggaaaa
     6421 agctcactac tgtttcacca aagtctattt ctgtatgctc atctaccaca tggaaatcct
     6481 taaatccttt ggctgcttca ttacgactga cattcaattt tgctaactcg atcgtcaact
     6541 ctgtcccgaa tactggtaca gaaatttttg acaacaagta aggaagtgcc ccgatcgcat
     6601 ctgcatgtcc atgtgttaaa aagacaccag ctacacgatc aatgttttct tctaaatacg
     6661 taaaatcagg gattacgacg tcgatcccca acaactcatt ttcaggatat tttagcccac
     6721 aatctaaaat aaaaatatcc tcgcccactt ctgcgacata catgtttttt ccattttcac
     6781 gaacgccgcc taatgggata attttaatgg tactcacaaa cttcacctct tcttatgaca
     6841 tccaagttta aagatgtcga ttttcttctt tatctaaata tatgagccat tccaatgaag
     6901 catatgcaaa atcactattt ctcaacttat ggagaatcag caggaaaatt accagcacat
     6961 ccgcgttctg acagttggat gggcatttta ttttggatat acttcattaa caaaatggat
     7021 cttttgcgaa acgctatagc cagaatactt atataagtat tcagctgttc cccattatta
     7081 tacaacaaat aaactaaatc gctagtatca gcaattgtta gaacaactat gcacgtaaaa
     7141 atccctctgt agcatctagg atttcttttg ctattcctct ttcatttctt cttcgtcggt
     7201 ttgaaacata cttttttcat tttaccacat tttttgcaat aactatagtg actttttata
     7261 tatacagaac ctcatgttga tggtgtgcaa acagcataag atttaagcta gctttaagat
     7321 aggttgtcta ttatcaaatg catctactag gagggatgca aaatatgaat tttatgaaac
     7381 gtgcatggaa aagtacaaaa gtaaaatggg gacgttcatt gttattgttt gccgtgttca
     7441 cagccattct tatttttgtg ctagctggcc tcacgattcg aagcgctgca gaagaagcag
     7501 cgaatcaagc acaaaaagaa gttggagcaa cagtgacttt aagtgccaat cgagaagctt
     7561 catttaaaaa acaggaacaa acttcttcaa gcggttctac cactgaagaa cgtccagatc
     7621 caggcagttt ttctcttaca ccagtttcct taacggacgc ggaaaaaata gctgaattag
     7681 ataatgtaaa gagttattcc tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa
     7741 ctgccatcac tagttctgat tcaagtgaag acagttcaga aactactgat gcaggcggtg
     7801 aacctgctgg aggaatgggc gggaatccgc aaatgactca agcagacttt caaatcagtg
     7861 gtgtgtcagc aactgcacaa aatagtaatt tttctgatgg aacagcaaaa atcacggatg
     7921 gtgaaggaat agatgacagc gacaaaggca caaacaatgt agtcattgat tctactctag
     7981 cagaaacaaa tgacctggca gtcggagata ctttcacaat cacgagtact gaagatgaag
     8041 ataccactta tgaaatgacg atcaaaggaa tttatgaatc cagcgaaaca agcggcagta
     8101 tgggtatgaa attcaacttc atgaatccgt cgaatacact ttacacttct tacacttttg
     8161 caaatgaact aaatgggaca tctgacgata acacgataga ttctgctatt tacactctat
     8221 ctgatccaga taaaatggat gagtttctca aagaagctga gaagctgatc gacacagata
     8281 catttagctt acaatcaaat gattccatgt atcagtcaat gttggaacct ttaaacaacg
     8341 tggcaagttt ttctaaaaat gttgttttac tagtgactgt agccggtatt atcatactta
     8401 ctctgatcat tatgattacc attcgggagc gtcggcatga gttaggcgtc ttgctatctt
     8461 taggagaatc cagaagtaaa atcattctcc aattgtttac cgaagtggct atttgtatga
     8521 ttcttgcatt aggagttgct tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc
     8581 agctacttga ccaacagaca gaaaccacga cacaaaacca gcaagctcct ggcggtggac
     8641 aaatgccagg taacagacaa gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga
     8701 ataatccatt tgctgtttct gatcaagtca gcgaattgga aatcaatgtc aaacctgcac
     8761 aattgggact attagccgga gtagcatttg gaatcagtct gttttctgtc tttttagctt
     8821 ctatagggat attaagatta aacccaagaa aaatcctatt aaactaaagg aggaatttga
     8881 tgttacggac agaacaattg ggattttggt atcaagatcc gcaagatgcg ctttataaag
     8941 atgtagaact gaatttcgaa gctggaaaaa tgtacgctat tttaggagca agtggttctg
     9001 gaaaaacgac ttttctttcg cttatttctg gactagatgc accaaaagaa ggaaaggtat
     9061 actatcaaga tcgttcactg aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct
     9121 cgattatttt ccaagcttac aacttgcttc cttatttatc ggctctagac aacgtcatag
     9181 cagcaatgga aatcactaag accaagcaag agaattatcg acaagttgct ttagatcgac
     9241 tggctcgtgt aggaattgat gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag
     9301 gtcagcagca gcgagtcgct atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag
     9361 cggatgagcc tactggaaat ctagatgaaa caacttcaca agatatcgtc aaacttttcc
     9421 aagaaatcgc ccatgaacaa aatcgctgta ttatcatcgt gactcatgag attgaagtag
     9481 caaaaagctg tgatgaagtc tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt
     9541 aaaaaacagg ataatgatca aataaaaaat ccgaaccata tctgtctcgc agatatggtt
     9601 cggatttttt atttgcttga ttcaattagt aataagtggt ttttattttt tttcgtcttt
     9661 tcgagaaaac ttgtctttga cttcgtcgcc taaatttcga gctttttctt tgatgtcagc
     9721 acctagtgat tgtgctttcc cttctgtttc tttgctcttg tcgtctgttg ctttgccgta
     9781 tgcttctttt ccttcaccga ctactttatc tttcatatct ttagaacgtc cttttaaatc
     9841 tgccattgtg cttcctcctc ttagtttgtt tattgatcta ccttcagggt aacatcaaca
     9901 acagatacag acaaacagaa cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa
     9961 aaactttttt cgaaagtccc ccatactttg acttcttttt cttggaccat tatttctcct
    10021 ttagcgataa ctgtatcaat atcaagcgtt tctttatcca agatacacag atctgcatca
    10081 gcaccaattt cgatttttcc ttttttatcc aatttcaata tacgtgctgg attactggtt
    10141 atagctcgta aggcgatttc caaagggata ctttcttttt gcacagcttc tttgatccct
    10201 actagcaatg ccttggcact tcctactcct aaccctagga aatgattgtt ttcatcaaaa
    10261 taaggcaagc ttccttgtcc atctgaggtc atcgtgaaat tatctaaaga aacatcttcc
    10321 tcgatcaatc gtttcagtgc tttgctgaag cgaacttcac catctgtctt ctcccaaaaa
    10381 tcaggatctt cgctagcagt gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa
    10441 gcttcaaata attctggtgt gcgatttgca tgagtaggca aaaattgtgt gatcggaatt
    10501 tctgtttctt ctacgattcg tgtcaatagc tctagctttc tttttcctcc gcctaagtgg
    10561 acattgatga tcccagcttt tccggataac atgcctccta cacgagcatc tgctactgca
    10621 cgagcgaact cttcaaaact tggctgagaa gaacggtgat cggatactgc gatttctccg
    10681 attccgataa ttttatcaat tgtcaaaaag tctttgatga tagaatctgt tactgttttt
    10741 accggcaatc ggtacgaacc ttcgtataca taagtggaga ttccttctgc ttccagacct
    10801 ttcgctttgc tgatcaacgc agtcatatct ctaccttctc cgtctgttcc caaacaaccg
    10861 acaactgtcg ttacaccagc tgtagtcaat tggctcaatg tgacttctgg cgttcgattt
    10921 tgataaccgc cttctcctcc tccacctaaa atatggaaat gacagtcaat gaaacccggg
    10981 gttagtactt ttccctcacc tgaaagttct tctacttcca ctccttcgta gccgccttct
    11041 agctgttctt ctacagccaa aatcttccca ccggcaatca gtacatccat ttttcctaag
    11101 taatctggtg caaacacttc aatttgtcga atgattttca tacatttttc ctctttcttt
    11161 aagtataact gatgatagga tcttaaaaaa taatccaaac gataggatct ttactccttt
    11221 gattttaccg taaaatgacg gttcttggag tccttatcta ctgtttggat tactttttta
    11281 tttacaggtc tccagtacta ctgcagacct attgcaatgg caatcatgat gaaaatagtt
    11341 cctaaaacaa acaggaatcc ttgcatcttg atttggaatc ttgcccattt tgcccattcg
    11401 atccgtgcaa cacctagcac accaatcaag ctggctgata cgggtgtaaa agcgtccata
    11461 aaacctgaac caagctgata agcaagaact gctacttgtc tggatacacc tactaaatct
    11521 gccagtggag ccatgattgg catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc
    11581 aagttgaaca aactttggaa gatgtacatc gcccaagcac caatgacagc tggaacaccg
    11641 gtcaacgccg tcccgatccc atgcaaaatc gtattcaaag ctgaaggaac agatgcatca
    11701 gatcctccca agacaagaag aatccctttg gccataccta ctacgattgc tgtaccagct
    11761 aaatcagctg caccagattg gaaagaagaa gcgatgtcat tgactttcat tccatctaat
    11821 ttgaaaagaa cagcaatgat tccagcaact aatcccatca caaagaattg agaagcgatt
    11881 tctggaatgt aatagccttt ttgagtcacg ccccaaacga tccagacaag gacaactaac
    11941 atctctaata agatcaattt atgcccaagc atgaattttt tattttcatc agatgtcttt
    12001 ttgatatgag aacggaaata atcatctgat ttgtaagtca acgaactttc tggtttttta
    12061 cggatttttt ctgcatatat catcatataa ccagcggcta atgcagtaac aactacccac
    12121 ataatcaatc ggaatgtcgc accagacaac acggggattc cagcaatccc ttgagccacc
    12181 gctacgctaa acgggctcat ccaagaggtc gcatttccga cttgagatgc gacataagtc
    12241 acggttactg ctacgatcga gtcatatcct aacgcaatga caaatggaac catcaccatt
    12301 gagaatggaa ttacctcttc tgccatcccg aatgtagcgc caccgaatga aaaagcaaag
    12361 aataatagcg gtaaagctaa acgctccagt cccttagttt tactgataaa cgcatagata
    12421 cctgcgtcga tcgctccggt acgcataata ataccaaaag cacctccaac aacaaggatc
    12481 aatgcagcga tccctacagc agatccatac ttatctccag atactagtcc ttcaaaaaca
    12541 aaatttaaga aaccaaagcc gccaaaatca tctgttcccc atattttggc tgttttatgc
    12601 aattttttag aagtatcata gatattttcg ccgtactgat cgtaaagcac atcatctgtc
    12661 aaggagattt catctagttt ttcctgcgta agattttttt ctccgtcagc aatgactttt
    12721 tccaatcctt tttccggaac atccagtttt tcacgttctg ctggatgatc ttgcagttct
    12781 tctaattgat caaacacata cgacttatcc agctcgtaag cataacggaa ggaatcttga
    12841 cgtaaaacag tacgagtact tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg
    12901 ctgaactttc ctgctggaac gacaaaagtc agtatccatg caaagatgac tacaccaaaa
    12961 ataatgacat acgtatgggg tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa
    13021 gaatttgttt tcttcaaatt tatttcttcc tttctttttt cagtaatttt gatgaataaa
    13081 aaatggataa tcaaatgaat tatacagtaa aaatgacatt tccacatgaa taatttatta
    13141 gaaaatcact tttttctatt ttgttttttt tctcctatgt atgattattt ttttctcttt
    13201 ccatttatat taagtatttt atttctctgt ttttccttaa aattcttttt ttatttttag
    13261 attagagaaa atctcgtcct gtttttttca ttttacaatc attgaaaaat tgaatttcat
    13321 tgcaaaaaaa ataataaaca cttataaata tccattatct atccttcaat ttttgtgtat
    13381 tttatttcta ttagaaaaag gtatcctgtt ggacaatttt gttatatcag gtaaacttca
    13441 ttgcaataat gataacaaat gtactataat tactttgtaa agagttttgg ggaagactct
    13501 ttcatcaacg tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt
    13561 tccactactt tttatttttc tgacactaca tattcttata actcatttct cataactgtg
    13621 aaacaactta taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca
    13681 tcttaaaata actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc
    13741 tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca
    13801 tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac
    13861 ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt
    13921 tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa
    13981 ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc
    14041 agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc
    14101 cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg
    14161 atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc
    14221 aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact
    14281 tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca
    14341 agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata
    14401 atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag
    14461 aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt
    14521 ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat
    14581 agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc
    14641 tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc
    14701 agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag
    14761 ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc
    14821 aagcaagaaa atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa
    14881 aggaatggct tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg
    14941 aaaaaagctt ttttccacca attaaataaa tctctcatct caatagttta tccattattt
    15001 tatctttttt tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt
    15061 tcgagagaat ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac
    15121 tttcttttca tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat
    15181 acgcaagctt cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat
    15241 gctgcatacc agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata
    15301 aatagactaa taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac
    15361 ttgagtatat caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac
    15421 ctttttttat aattaaaaaa ttcgcctttt tattctacta tgaatcttct ggcttggctt
    15481 tcatttctct tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc
    15541 ataatatttt ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca
    15601 actgatctag agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct
    15661 ttaataacaa atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc
    15721 ttagcttttt actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt
    15781 gaagtccata ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt
    15841 ttcctttaaa ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg
    15901 ttagctgtat ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag
    15961 gagtcagtag ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt
    16021 tttctacaaa cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa
    16081 ttccgtattt agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg
    16141 tttcttcata atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat
    16201 cccccagcat gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat
    16261 cccccagtcc tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt
    16321 caactaaagc gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat
    16381 ttgcatcaaa gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt
    16441 cgattcccga atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg
    16501 gaagcatttc ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg
    16561 caggaatgac tgcttttttg accttcactt gct
//
LOCUS       DAPBCS010000062        16549 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPBCS010000062 DAPBCS010000000
VERSION     DAPBCS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.064"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..241)
                     /locus_tag="RD521_002397"
     CDS             complement(<1..241)
                     /locus_tag="RD521_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="initiator RepB protein"
                     /protein_id="HDT7772079.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNP"
     gene            1222..2025
                     /locus_tag="RD521_002398"
     CDS             1222..2025
                     /locus_tag="RD521_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7772080.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2610..3065
                     /locus_tag="RD521_002399"
     CDS             2610..3065
                     /locus_tag="RD521_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772081.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            3184..4242
                     /locus_tag="RD521_002400"
     CDS             3184..4242
                     /locus_tag="RD521_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDT7772082.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            4284..6233
                     /locus_tag="RD521_002401"
     CDS             4284..6233
                     /locus_tag="RD521_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDT7772083.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            6236..9877
                     /locus_tag="RD521_002402"
     CDS             6236..9877
                     /locus_tag="RD521_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772084.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            10277..10615
                     /locus_tag="RD521_002403"
     CDS             10277..10615
                     /locus_tag="RD521_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772085.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10725..10946
                     /locus_tag="RD521_002404"
     CDS             10725..10946
                     /locus_tag="RD521_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772086.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            10966..13122
                     /locus_tag="RD521_002405"
     CDS             10966..13122
                     /locus_tag="RD521_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772087.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            13134..13367
                     /locus_tag="RD521_002406"
     CDS             13134..13367
                     /locus_tag="RD521_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7772088.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            13357..14223
                     /locus_tag="RD521_002407"
     CDS             13357..14223
                     /locus_tag="RD521_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772089.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            14238..16208
                     /locus_tag="RD521_002408"
     CDS             14238..16208
                     /locus_tag="RD521_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772090.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            16375..>16549
                     /locus_tag="RD521_002409"
     CDS             16375..>16549
                     /locus_tag="RD521_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772091.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         6104 a         2338 c         3281 g         4826 t
ORIGIN      
        1 gcggattttt agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat
       61 ccatttcaac aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt
      121 ttagattgct ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac
      181 gattgcctct tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca
      241 ttgaataagt atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata
      301 acataactca atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata
      361 cagcagaaat cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga
      421 aattataaaa attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc
      481 acatactttt tctttttttg gatatattcg gaataccaca tcgctttttg tccacgtttg
      541 catcgctttt tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca
      601 ccctaaaatc tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga
      661 ttaaaatata tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt
      721 ttttcaatta ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt
      781 aagatttttt atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat
      841 ctgttaatat atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg
      901 ggagagtaag tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag
      961 tggacagtaa gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat
     1021 cacactagga taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa
     1081 ttagattagt aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac
     1141 aagcagaaaa aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa
     1201 aagaagattg gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat
     1261 gtagatttag tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca
     1321 acagctttgc gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa
     1381 atgaaacgtc aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc
     1441 aaatacgaca gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa
     1501 ctaggtaatt taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt
     1561 caaaaattcc ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt
     1621 gaatttatct taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca
     1681 aaactaagaa gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa
     1741 gagtggcgaa atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata
     1801 ccttcaagtt atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa
     1861 tttttagctc ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc
     1921 tatgcaaaga aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca
     1981 aacttgccaa ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt
     2041 aattttaata caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caataaatgc
     2101 aagaagtttt tttgtaagca agtttcagtc taaataatat ttgaaagaat ggctaatcta
     2161 tacaaatttt aacaactatg ggtaacactt ttggacaagt caagaaaaaa aacaattttt
     2221 cttaatttgt tgatcgacta aaggtaacac ttttggacaa cacagaataa aaagtccggt
     2281 gatatcagtt ttggtaggaa acatcttaat atctttgtga gattgaaggt aacatttttg
     2341 gacaagtcaa ctatttgtta aaagttaata tagagtaatt agataagttt tttcttttcg
     2401 tctgataaat ggataatatt agttatttta ttgctaatta gtctttcatg atgactgaaa
     2461 tagctaaaat aaatatctcg ttggataatt gctgatactt taagggatat ctaaatttgc
     2521 tgattgattt atttatgtta ttatgatatt ataataacat aaatgtgtta cgcccgaacc
     2581 tcattttaaa aaaatgaagg ggtgagacaa tgaatgctat taattggcat gaatttgtta
     2641 actatatgga gagcttacta gatgatctaa aaagttttag tattgctgat gcagtaaagc
     2701 atgggttacc agttagtaca acgatgaaat tacgaggatt gaaaattcca gatgaaatta
     2761 ttaagctgag aactaaaaca attattgaaa ccgcaaaagt cgttgacaat atgaaccatg
     2821 aactaaaagg caagaaaaca acttacgaga aagattttga acgtttttta aaatctgtta
     2881 ttggaaaata tctaggtaaa aaatctttca gtgaattgag agatgaagag attgtaccta
     2941 ctccaatggt tagcaaaatc cgaaaaaaca aagccgaggg gaattactat cttggtacaa
     3001 tagcaaaaat cattgattat ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa
     3061 gataattttt gtgttaaata ttaatgggga agaatatacc gaaatgattc aaaatcccga
     3121 acctagaatt accgaaaaaa cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa
     3181 ctaatggaaa aaaacgtcaa ttcaacatct accaaagatc aatacagtat tattggaggg
     3241 ttgcttggtg ataaggataa gagaacacgt cacggattac gcattatgct tgcaactttt
     3301 ctaggagtgc tttttttatt aacaggcttg ggtagctaca tttatcaatt aggaaactat
     3361 aattattatc tcgtgaacaa tacaacacct ataggctctt cattggagtt tgctcgatca
     3421 ggagcagaga tcactatttc agatgtgtgg acagatcaaa acagagatgt aacagtagta
     3481 aaactcggtt actcgcaaaa cgctagaaag ttgttatcta cgcaaggaaa aaactacaac
     3541 ttatatatgg caacgaaaga aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg
     3601 ttaagtactg atggggatgc tttcttattc ctaaaaggta aattagatga aagagcttat
     3661 caagttttta ttgcaaacca agtatcacta atttctggtg atgatacagc tgacgatgat
     3721 accaaagtca atgtgaatga agcagattca gttacaaaag cactttctca atattctatg
     3781 aatgatgtta ataatgaagg agtaatttct tttgggaaaa aagcaaaacc tgttaatact
     3841 cctgataata tcaatttcag aataaatcca tattcagatt caactaatgt ctatcaagga
     3901 agctttctta ccaaagatgg cgatattgat tattcaaaag ttgtagcagt tacaagtaag
     3961 aataatgttg taagtaagtt agaagatcag ttaaatgctt caaatactaa catcaagaaa
     4021 attgaagcta caaaagctga gtatctggaa cgtttacaac aaaatcctga cgataaagat
     4081 tcgcaagaaa gtattcgtca actagacagt cgaatggaga ccgaaattaa agcaatcaat
     4141 gatacacaaa aattgattga tcaatacaaa aatgctgact ttaacaaaga ttcttttgga
     4201 gacatgaaga cgacatttaa gtacctaact ctgactgact agaagtaaat tattcttatt
     4261 ttagaggaag tgaacacaaa cagatggaaa atagtaccga gttttttaag aatattcaaa
     4321 ctgaatttaa tcatgctctc acaccaatga aaccaccgaa acataagtta gtgggacgtg
     4381 atgacgaact ccatgagttg tcactgatct tgaatagacc ggaaacaccg caagctatct
     4441 tgttaggtga agccggaaca ggtaaaacag cacttgcaag tgaatgggct gaaagacagc
     4501 gacaagatgg aaagaaagta caagtataca aattatcaat cggacaattg cgtgttgggg
     4561 gagatgatgt cctccaaaca cgtttagaaa cattaattcc aagattgaaa gaatatgagc
     4621 gaattttaaa atctgaggac aaagaaaatg aatttgtcct cttcattgac gagtttcata
     4681 aagttgttag ctcttttggt agtggcaata aaattggggg tgattcgcta aaagaaccgt
     4741 tagcaaatga atcacttcaa ttgattaccg ctactacaag aaaagaatat gaaaactata
     4801 tcaaaagaga tgatgcctta gggagacggt tacaaccgtt agaattgaat gaactaaaaa
     4861 gcgatgttgt cgtcgatatt ttgagacaat ggctaaatac atcctctgtt aaagatgatc
     4921 ctcaggcttc ttttaaatta tcgcaaagga ttcctgatga attacttcaa gaaatcgtac
     4981 aagcaaacaa gctttatcgt gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg
     5041 aggatttgat tgcaaaagat cgagtagacg gaaatgctta taatcgagaa agtctattgc
     5101 aaatatttgg taagtataat attcagctag attctgattt tgatatggaa aaacttcaag
     5161 aggaattaaa gtccataaaa gggcaaccgt tagcagttta tactataaca atgttgttta
     5221 aacgtatgaa gcttggattt gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc
     5281 tgtttacagg atcaacaggg gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac
     5341 tttatggcga tgaaaataaa gttctcttag ttaatatgcc ggactacaaa acaaaagatg
     5401 atgcaatgcg ttttagacga ctcataggta ctcatgtaag gcatgatcct tctgcgatta
     5461 ttgtgtgtga tgaatatgag aaagctggtg tagaaacact cgatgccttt ttatcaattt
     5521 tagatgaagg taggataagt tttactgaaa gaggatggga tgatcgagaa gttcacgaca
     5581 ctgtttcatt gtcaaataca attttatttg cgacaacaaa tgcaggtgcg gagatgttcg
     5641 attcgttaaa cagatatagc tcactatctc gtgttacaaa caaggaaaat tctaatgaat
     5701 atagcactga actaaaaaga gaataccgaa aagtcgcatc agggatcaaa caagccttga
     5761 ttggtgaagg tctaaagcct gaagttatta accgatttgg acgaatagtt ccattttggg
     5821 ctttatccga acaaactctt ctggaaattg cttataatga aatgttgaag agtgctgaat
     5881 tagtacgaaa acatttaaat atagatttac gtcttccacc taggaaagaa agagtatatg
     5941 gtcaaaattt ttatgcagac gatctctcca tgcacttagt ttttgacatg gcttcacaag
     6001 aggattctaa atcagggggt gcaaggaata tcttgcattt agttgaatct gagtttgaag
     6061 aagcgattac tgaagcaata attacttatc caaacgcaaa aatattcgat gttaagttga
     6121 gtgaagccgg agcgtttgaa ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt
     6181 ccgttgctga aagtaaagaa aacaagattg tattttcgat tggaggggcg tagctatgaa
     6241 attcaagaaa tatttactac aaaaccttag aataaaacga tgggtcgtaa gaggtctagt
     6301 tattggagga gtattagcag ttactttagg cgctcctttt ggaatgaatg atccaactcc
     6361 tgacaatcaa gatagcaaac aagaaacagt tataaataag aacgttgcat atgcggaaag
     6421 taaaccggca gaacaaggaa tgtggacact atatgctcgt atcctaatgg gagataaaga
     6481 agttaatgat agtgccgcta aaactggaca aatgaatttg ttaggacaag gtggtatcag
     6541 tgccgatttc ccttattctg acatttctaa acaaggtaat tcgattgcag ggggtaatgc
     6601 aggtactcaa ctagcatcta ctttagcaac ttatagttac tacggatata ttgaaacagt
     6661 ctcaggaaat gctattgcca gtcaagcatc atccattcta ggaagtattg gtaggacgat
     6721 tggtagtttt atcggatcaa ttagtttaat cctttctaat attgctgaat ccataaatga
     6781 actccttgga aaatttttca tgagtatgaa tatattttct cttatggggt ggggagaagg
     6841 tggatcggat gtatctaatc cggtatctaa agcattaact agcttgctta acaatttggg
     6901 attaaataat gaattattta aagcgatagc aagcttatct atgctctttt tcacagcaat
     6961 actaggttat caaggaatga aattattgtc gaaagatggt gttaactcga agggattatc
     7021 accagtactt gaaaaatgga ttgttcgtat ttttgtattg tttatattct taccagtggc
     7081 aggatcgcta ttgggagttt atatcaacgg catccagtca gcaaaaaaag atggagatat
     7141 accaacaaat gttgtttctg actacatcat taacacaaga ctttgggctt ctactcaaaa
     7201 tcttgctcca tcatctgata ctggtggtga tgtacctgac ggaaagccta catccgaaat
     7261 caagttcgtt gatacagatt atgaaccatc taaaaaatct gcaatgataa gagcgatcaa
     7321 tactagcagt tatgccagac aaaataatga tctagattct aattcagtcg gtatggctct
     7381 attaaaagaa tggtcatcga atgatacttt caatattaat acttatgccg gtgatgttaa
     7441 tgtcgccgaa aatgatgctt atatgggaag agcgcctgct gtttggacag gctataattt
     7501 aggggatgga aaagaaggaa gtgcaattaa gaaaccaacc aatttaaatg gatatatttg
     7561 gtcagcaaat caaaatgtga cagacgacaa tcgcaagcct gatggaaaag gaacagcgca
     7621 taactttgat gctactcaac cttggggcgt tgctaacaat gcaactttta gcacacagtc
     7681 ggtagcttta ttgctccaat cggcacttga tacatcggga gctaagtttt atgcgtataa
     7741 tatcgctcct tcaggaattc aaggcagtgc taagaatatg tcaacagtaa aaacagaatg
     7801 gcgtacagca tccttagttg gtcatggagc tatgggtaag tttggctcgt ggctagcgat
     7861 gaacgctgaa tcaatcgttt tagcagttct ttacttagca tgtgcttggg cgatgttcaa
     7921 tgtgaatatc gtgaaagcat ttaaacgtga atttattcgg atttgggaag tagttctaac
     7981 aggatcactt gcatcaggag caggtgtgat ggcaatttca ttaggtgttg ttacaacatc
     8041 tctgatcgga ctacaattac ctatctttct gattactttt attcgtgcat ttggtgatac
     8101 tgctgtggga ttcattccta atacattagt tggcgatggc gtagcaaata ttgttcgatc
     8161 tgctatgatt ttaggtgtag gatatctttt agtctttaaa aaaggcgaaa aagtaaataa
     8221 tttgattaat atgcttattg gcttaccact tgaagtagcg tttgggtttg atgaacgtgc
     8281 taagttatta tttggtaata gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc
     8341 aatcaagaaa agccaaggag ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa
     8401 agatatggtt gcaggaggtc ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa
     8461 caatatggga gatagcaatg ctgatgctaa gtccattgct gataaagtaa ttcctgaaac
     8521 accagaagga atgtcattca acggtggcgg agttggttcc acaatcaata atacctcttt
     8581 caaagattta gttcgtcaag atcaactacg taataactcc caacaatata ttgatcattt
     8641 aggacgtatg gttgatgaaa acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga
     8701 tagtaaatac ggtaaagatg ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga
     8761 tgaagatgga aaccttatag atactaatgg taaaccattg gaagatagtg aagatggtaa
     8821 tattggtatg gatagcgaag gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga
     8881 tggaaatctt gttgacgaaa atggacgcag gttgctaaat ggtgattttg gtcatgatag
     8941 taacggacgt ttagttgacg aaaaaggtcg tttagttgat gaagatggaa atcttgttga
     9001 tgagtttggt aataaacttg gtaaacacag cgatggatat aatgtgaatg gagatttagt
     9061 cgatgaggat gggaatatca ttgagacgag agatgcgctt gtggcagatc gaaatagaga
     9121 actcgaacaa caacggcata cgccaattgc tgttgcaatt cctgagaatg aagaagacga
     9181 gattacttta gatcaaactc atcaagaact gaggaatgaa atgagtggtg aggacgtgtc
     9241 agacagagtg catcgggaat taggagatgg agcatctggt gaaagtcagt caagagagct
     9301 taacagcaac tttgataata gtgctattcg gaatgacaca gtacttaaag acagagaatc
     9361 gctaacaaaa gaaacggata aagaacaatc agatgtgaag aaaaagttca ttcctactcc
     9421 taatgttaat caaacagata aatatgaaac aaatattgga tcaggaatgt cgaaagacaa
     9481 tcaatcaatt agagaacctg aactgacaaa taaaaataat agaacaaata attctaaatc
     9541 tacttcagat aattcaggga aatctaatca atcaaccaag aaaacatcaa cagctaaatc
     9601 agccgttgaa tatctatcaa aaactggtaa aaatgtttac acctcagcag ataaagcttt
     9661 agcaaaagct ccaaaaggta gtctagcagg gataacctat gaagctaaaa acgaagctat
     9721 taaaataggt aaaggaacgg ctaaggttgc caaggctact gctacaggag catccaaaac
     9781 tggtaaagta gtgaagaaat ctaccatgtc agtcaaggac tatattgtaa aatacggtaa
     9841 tcaaaaatca actgaaaata agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc
     9901 cttcttttta ctttgatttc taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa
     9961 aattatttta aattgtctat aaatgaatta aatatttact gttcaaagtt gtataattaa
    10021 aaagggctta tgctggtaaa gaacagaatt attatctaaa caaaaacttg ataaaccagc
    10081 atttttagcc aataaaaaaa gtgaaataat ttaatggaaa atctctttta atcatcgaaa
    10141 atttatgtta ttatgataac ataaagatgt aaatatcgag acgtcttttt cttagttaga
    10201 tcattgacaa gtgaacaaat aagatttgtc gatgatataa ttaacttatt aaaaatatta
    10261 aaaaggaggt ttttatatgt tcgattctat taaaaatttg gttagcggaa tgacgcctga
    10321 tatgaaaact ttagtacaag ttgttttgta tttcttggca ttcgtttttt cgttgaaacc
    10381 aggattcact gcaatgaaat tttttggtga tgcaaaatgg ggagatggat taaaatatgt
    10441 tggggcgatt attgcaatct ttattattcc tgtaattaca accgttatgc tttacacaat
    10501 gggtactaaa acaggtaatg atttgaacca aaaagctaat atcatatcag cacttattcc
    10561 agttgttggt atctatctag tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg
    10621 aattgaacaa atttggataa tatgccgatt tgttcaattt actttttacc aaaaaactac
    10681 gctccaagcg tagtttagaa gtgagggaag aaagtaaatg taagatggtt tttgatagag
    10741 aaaaaatgtt agctcacgcc aacgaagtat taatgagcag tctgaaaggt accgaactgg
    10801 caaagataat gaatatgaat gttaaccagt tctatgatta tcgaaatggg agtaaaaaga
    10861 ttgaaaaagc cagacttgaa acgcttatta aattcgaaaa agcatatgta tatatgctag
    10921 ataaacagaa aagaactatt gactgaaaaa aggagtttta caataatggc aaaattcaaa
    10981 gatattaccg aatctattaa ttatgtggca gaaacagttc ctgtagttgc actctctttg
    11041 aagccacaat tactcgaagt gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct
    11101 aaagcaaagg tatggaaaga attcgatgcg ttgctgacca aagatttacc aaagaaaaat
    11161 tatatggtaa ttgaagatgt acaaaagctc aacagtgagg gagttataga tgaggatgat
    11221 gctattctcg attctgagaa gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct
    11281 gatctaccag aaaaatttaa agagaagcta ttagcaggtg tccgttttat ggatagtagt
    11341 ggtcaattat tagttattcc ggatgatcct aatgtgtttg aatcagaagt tgaagaagct
    11401 acagacttct atagcgcagt agagcaatta gaagtagcta ctaatggatt agaacgaatt
    11461 gctgagacct tcaactcttc tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca
    11521 gatgaaaaag aagctctttt tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa
    11581 gtaagcatag aagaaacaca aatacaaaca gaaacctacc aacaaccaaa aattgatgaa
    11641 aaacccgaat cccaaagtga caaagaaaat tatgtttatg aagctgaaaa aatcacatct
    11701 gaaagtgatg taccgaaaaa cttagatgct gattttttag aaaaaaattt tgaacagatc
    11761 attgctgaca aatttcaacc tgtagtttta gatgagatta atgaccctgt cgatttaaac
    11821 gaggagatgc tatccatacc tgagttgaaa ccattgctgt ttttagctaa agagagtatg
    11881 aacgctcgca tctctattgc taataataaa atcaacagtg aattaaataa gacgaaggag
    11941 caattgatcc tcaaaggaaa gcaaatgtta gctgatgagc ttttgcggat agaggaagca
    12001 acctcattaa atggtcaaaa tcagtttaca aatacactat cggttgctaa agagagctac
    12061 gaagcagaag catctaaaat tgatgattta gtgatggaaa aatccaatat cattcgtaaa
    12121 gattatgata tggcgaaaca ggcttatttg gatgctgaac taaagaaact agagcaacgg
    12181 tatgatgctg agcatttacc agaagttaaa caacaagaag atgaatatag aaaagcaact
    12241 gaagaaagtc ttgatcaaca gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg
    12301 aaacaaagcc aagaagcaaa agaagttgtg gtggaacgaa ttatcaattc attgaaacca
    12361 gaaattgaaa aatcattaac agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca
    12421 aagtcagaaa atgaagctga gttccatgat acaaaagaga aaacaacaga tgtggttgtc
    12481 aaatattttg cgaacaaaga agaagttgaa aagcaggtag ctaacaacac gaaagaatta
    12541 gaaacaaaac ttgtagagca acaaaaaact atcgaagaat ataaagatcg aatgaaagag
    12601 gtcaaagatt tagcagaggg ttataaatta aatagcgaaa agttggcaga acaaagtgaa
    12661 gctttaagag aagacttatc tcgttcccga agtgatctag ctcaaatggc agaaaaaacg
    12721 aaatcattag cagttgctac agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa
    12781 aagatggcta ctatcatttc tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc
    12841 tactttatta gtcataataa tcaacaagca caggctcaaa ctcaaacatc tatttcgaga
    12901 gtggaaaatt cgattgggac aaatacgaac tctgcttcaa caaatagtag ctatgaagca
    12961 acaccaacta cagagttcaa agttgggaaa gatgttgtca tcagtattga tgggaaaaca
    13021 actaaggctg aagtgatttc tattgatgga gaaaaagcag ttgtgaaaac tccagacgga
    13081 aatcagtaca aaattcaagc tcacttatct gataataact aaggagggct attatgcgag
    13141 aagaaaaaat agtttatcca gtaattcaaa acaatcaaga aaacatgacc tcttctcagc
    13201 acttacagga agcacagctc gtgtatcagg taaatgaact accacaaacg aacgagacaa
    13261 tgagcaagtc aatatcttta attggcttgc ttattgttgc aatagtgata actgtaggtg
    13321 tttaccgtaa gttgaacgtg aaaggaagta tttctgatgg cagataactc aaactatgga
    13381 tttaaacata acttacatca aaaaccttct gaaacaattg tcagagttga atggcttgaa
    13441 aagcatgtca atagcaccta ttctgttaca gcaaaaagat taattgtatc agctatagtt
    13501 gttgcagtcg ttgttcttgg atcaattgtt tacttagttt ctgatacgat taaactgaaa
    13561 tttatttttg ttccaacgtt attaatgata attggatttc ttattctaat tagccagtat
    13621 atgtttattg atccatctag tggttcattg atttctggtt ctattagact gttcaaatca
    13681 gtagttaatt cagcaagagt atctatgggg aaaaagaaac catttcgagg cacagagatt
    13741 ttaaaagtga aacaagatgg aacaatcgta tatcgagatg ggagtgtagc attaatgttc
    13801 aatctagatg gggctacatc actaaccgct tttccagaag aagttatggc tcaggaatcg
    13861 aaatcaatac aatatcaaaa taccagagat cgttccttag ctgaaatcaa aatcacttca
    13921 tctcaacctc agaatacgga aagacaaact cgaaatatga aaatacaaaa acgaaatgca
    13981 aggagtaaca aagcagtctt tgacataata gatcagcaag aacgatttgt atcagaatat
    14041 attgatggtg tgaaaacaac agtaatccaa tatcttatct tgaaatctca atctaagatg
    14101 gtattggatg aaggtgtgga acgtttagaa aactatacga gtagaggact ttattatagt
    14161 gtcactcagt tgtcagctaa acaaacaaaa gaaatattat ctgatattta tgcactgaaa
    14221 tagaggtgag agattaaatg gaaattatgg gtagatcaca actattaaat ttcaaaaaaa
    14281 agagaaaaac taaaaaagaa caaaaaacta gaaattcaaa ggtagtgaat aatacctttg
    14341 ttagtgattt aacatcttta gttggttatc aatttacacc atcttacatt aggacaggta
    14401 agcggtgggg aacaattgtg aaattagtga atacttatgg aatgaataga aattatggat
    14461 ttggttggat ggttaaagct attcctgaag tgagagtaga aggggtaaaa gcctatctaa
    14521 ttgaagcaga taagccagtg tcacaaaaag atcaacaaga aataatgcac gagaatgttg
    14581 ggggatcaac gaatagttat gaaaataatt tagaaggaaa aaattcagat gctgaaatta
    14641 gcaaaactgt tatgcggatt atggatttga atacagcatc tttactagac agcatggcaa
    14701 ataaaattat tgattggaag ctagaaatcc tattagtatc cgattctcct aataaaatca
    14761 gcgaacagct aattaaactc caacggttgt atgatgaaaa aatgtatgga ttaaagctta
    14821 tgtcatatgg aggagatcaa cgtgatctgt ttgatagact tttaagtgaa ccaatcggga
    14881 atgtctatga gtatacgtct atgagttcgg attttgcagg aaatgatcat gcaattcgca
    14941 aaggtttaga tgatgcgaat ggatgggcta tcggcactat gtctactagc tacgcaagtg
    15001 gtgtagcttt tatggcactg gatgattctt ttacaagtaa agttttagtg gcatcacaca
    15061 aaaacgctac aattaaaggc tatgatgaaa aaatctcagc atcatcactt tgggggcaat
    15121 tgattgctaa taatgcaatg gcaaatcata aacgaacttt tcatattgtc ttaaataaat
    15181 ttgattactt tggtgatcca actggtagaa catttgttgc caaaccttcg ttaaacagtg
    15241 taatagaaaa ggttaatttg gctgaagaag gtcttaactc tttggagctt tttggtaatc
    15301 aaaaggacat tggtgattta tatagacgga atctcgataa actaactcag attttttatt
    15361 tgttagcagg cagaacatta gattctgcaa ctagagtttt attgaaaaaa gaattaggta
    15421 acttttatat aagtcagaaa ctttgggata ttaattatga agaacgttca aaacttttgg
    15481 tgaacagtag tgatacaatg ccagttcttg gggattttat tagctatttg actaatgctg
    15541 aaacaaaagc caaaaaatct ggtactgaaa aagaagtcga tcgtattatt gtattaaaag
    15601 acacattgag aacagcatta gacagcttct ctaggatgtt taatcaaaga acgacactga
    15661 aagaaatcgg taaaagtgac aaattacagt tttactataa cttaggtgat ctaagagcag
    15721 aaaccaatgt tatggaagct caatttttaa atacctttga tttcatcaca tcacaagcaa
    15781 aaaaagatga tattgttatg attcacggct tcgataagtt atctattgaa accgttgggc
    15841 taattaaaaa aagattaaca atgcttgaag aagatggaat tcgtcttgcc tttctatttg
    15901 atacaatcgg ttcaggagaa gacaatgaat acaaagaaga tattgaaaca gctgatatat
    15961 tcaatacaga tggaaaaatt taccaaagtt ttgaaagtga ttttaattac actatcattg
    16021 gaaagatgac cagtggagag ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag
    16081 gagaagatga tcaaaaactt tctgttgatt tgagacagta tttgacaaca ggaagaagta
    16141 ataaatttca gatcaggaga ccagtggatc tgactaataa ttttgttatt ggagagttta
    16201 ttctttaagt tttatctttg attttgagtg tataaggaaa ggataagaaa aaacatgtat
    16261 atttgagact aaaaacagtc ttagagaaca tggtacgttt tgtgaaaaga gaactataga
    16321 aatttcataa agtgaaacct tactgagtga aataattata gtggaggtta aataatgaaa
    16381 ccaaacataa aaattttaga tcgaatattt ctaggtagag acacagaagt aatcctaatt
    16441 caacatgaag aaggatttga agttagtata ggtattcaga aacttcaaaa acctcattat
    16501 tgtaatcagt tgtataaaaa ctttactgat gaagaaaaag cgagagttt
//
LOCUS       DAPBCS010000063        16075 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPBCS010000063 DAPBCS010000000
VERSION     DAPBCS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.065"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="RD521_002410"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="RD521_002410"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="RD521_002411"
     CDS             complement(227..697)
                     /locus_tag="RD521_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDT7772092.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="RD521_002412"
     CDS             complement(856..1314)
                     /locus_tag="RD521_002412"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDT7772093.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="RD521_002413"
     CDS             complement(1485..2630)
                     /locus_tag="RD521_002413"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDT7772094.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="RD521_002414"
     CDS             complement(2643..3560)
                     /locus_tag="RD521_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDT7772095.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="RD521_002415"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="RD521_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDT7772096.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="RD521_002416"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="RD521_002416"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDT7772097.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="RD521_002417"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="RD521_002417"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDT7772098.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="RD521_002418"
     CDS             7086..7967
                     /locus_tag="RD521_002418"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDT7772099.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="RD521_002419"
     CDS             7964..8680
                     /locus_tag="RD521_002419"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDT7772100.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="RD521_002420"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="RD521_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDT7772101.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="RD521_002421"
     CDS             9397..10398
                     /locus_tag="RD521_002421"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDT7772102.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="RD521_002422"
     CDS             10395..11756
                     /locus_tag="RD521_002422"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDT7772103.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="RD521_002423"
     CDS             11716..12867
                     /locus_tag="RD521_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDT7772104.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="RD521_002424"
     CDS             12842..14011
                     /locus_tag="RD521_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7772105.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="RD521_002425"
     CDS             14186..15565
                     /locus_tag="RD521_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7772106.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16075
                     /locus_tag="RD521_002426"
                     /pseudo
     CDS             15846..16075
                     /locus_tag="RD521_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5256 a         2840 c         3428 g         4551 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DAPBCS010000064        14317 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPBCS010000064 DAPBCS010000000
VERSION     DAPBCS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.066"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..99
                     /locus_tag="RD521_002427"
     CDS             <1..99
                     /locus_tag="RD521_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7772107.1"
                     /translation="ASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(277..2274)
                     /gene="tkt"
                     /locus_tag="RD521_002428"
     CDS             complement(277..2274)
                     /gene="tkt"
                     /locus_tag="RD521_002428"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDT7772108.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2376..2627)
                     /locus_tag="RD521_002429"
     CDS             complement(2376..2627)
                     /locus_tag="RD521_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDT7772109.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2774..3397
                     /gene="lexA"
                     /locus_tag="RD521_002430"
     CDS             2774..3397
                     /gene="lexA"
                     /locus_tag="RD521_002430"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDT7772110.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3577..3795
                     /locus_tag="RD521_002431"
     CDS             3577..3795
                     /locus_tag="RD521_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772111.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4084..4596)
                     /locus_tag="RD521_002432"
     CDS             complement(4084..4596)
                     /locus_tag="RD521_002432"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDT7772112.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4610..6907)
                     /gene="recJ"
                     /locus_tag="RD521_002433"
     CDS             complement(4610..6907)
                     /gene="recJ"
                     /locus_tag="RD521_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDT7772113.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7049..7492)
                     /locus_tag="RD521_002434"
     CDS             complement(7049..7492)
                     /locus_tag="RD521_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDT7772114.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7493..8281)
                     /locus_tag="RD521_002435"
     CDS             complement(7493..8281)
                     /locus_tag="RD521_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7772115.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8278..9219)
                     /locus_tag="RD521_002436"
     CDS             complement(8278..9219)
                     /locus_tag="RD521_002436"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDT7772116.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9396..9983)
                     /locus_tag="RD521_002437"
     CDS             complement(9396..9983)
                     /locus_tag="RD521_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDT7772117.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10204..11511)
                     /gene="obgE"
                     /locus_tag="RD521_002438"
     CDS             complement(10204..11511)
                     /gene="obgE"
                     /locus_tag="RD521_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDT7772118.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11805..12815
                     /gene="gap"
                     /locus_tag="RD521_002439"
     CDS             11805..12815
                     /gene="gap"
                     /locus_tag="RD521_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDT7772119.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12900..13835)
                     /locus_tag="RD521_002440"
     CDS             complement(12900..13835)
                     /locus_tag="RD521_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7772120.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            14021..>14317
                     /locus_tag="RD521_002441"
     CDS             14021..>14317
                     /locus_tag="RD521_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772121.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         4010 a         3180 c         2524 g         4603 t
ORIGIN      
        1 gcgtccaaaa gaaaccatat ccctagaaaa tcactaaatt accaaacacc attagaagtt
       61 tttttgagtt acgtaaatgg aaagttttgt ctcgcttaat ttgacaaata atattctcaa
      121 gaaacacaaa ctaatccgaa gaattctttg ttttgtcatt taaatgtaac atagccgaaa
      181 acgaaaagta atattgattt attcttctaa cacataaaga acagctgtct ccaacttgca
      241 gacagctgtt cttatcagtc aaacaattgc ttggatctat gatagttgat tgtacgtatt
      301 cacgacattt tctactgtaa atccaaattc ttctaaaatt ttagtgccag gggcagatgc
      361 accgaaatga tcgattgtaa tcgttgcgcc ctctgttcct acgtagcgtt cccatccaaa
      421 tgatgcagca gcttcgatag ccacacgttt cttcactgat ttaggaagta cactttcttt
      481 atattcagaa gattgttttt caaagaggtc aaagcttggc atagaaacaa cagaaacatc
      541 tttgcctttt tcagccaatt ctttttgagc tttcacagcc aaatcaactt cagaacctgt
      601 tgcaatcaaa ataccttctg gtgtttctcc ttgtgatgga gaaagaacat acgcgccttt
      661 tttcaccatt tcatctgcta cttcttttgt agaaggcaat actggaaggt tttgacgact
      721 caatacaagg atagtaggcg catctgttgt ctccatcgct actttccaag cagcacgtgt
      781 ttcatttccg tctgccggac gaatcacttg aacaccaggc atgcagcgaa cagatgctag
      841 ttgttcaatc ggttcgtgcg ttggtccgtc ttctccgaca gcaaccgagt catgtgttaa
      901 gacgtaaaca acaggagtat gttggattgc tgctaaacga actgctggtc ttagataatc
      961 gacaaagaca aagaatgttc caccgtagat acgagttccg ccatgaagtt ggataccatt
     1021 catagccgaa gccattgcaa attcgcggac accgaaccag atgttgcgtc cttcataatg
     1081 ttcaggagtg aaatctttat ctgcagccac cattgtatta ttggaacctg acaagtccgc
     1141 tgaaccaccc cagaagcttg gtatagcttt cgataattct tggatgactt ctttacttga
     1201 aacacggctt gcttgactgc ttccaacttc ataagttggc aattctgcat cccagttttc
     1261 tggtagtttg ccatcaaaag catcttcaaa ttgttgtgct aattcaggat aagctttttt
     1321 ataattggca aacatttcat tccatgcatc ttcagctttt tgtccttctt ctatcatcgt
     1381 ttgatggaag cgggcagcta cttcttctgg aacagtgaaa tctggatact cccaaccata
     1441 aacttcttta gccatcttga tgccatcttc accaagagga gcaccatgaa cagcagatgt
     1501 tccttcttta ggagcaccat agccgatgac tgtttttact tcgatcaatg ttggtttgtc
     1561 tgtttcagct tttgcttctt caatcgcttt agagattgct tctaggtcat ttccgtcttt
     1621 cactaagata tgttgccagc catatgcttc atagcgagca ccaacgtttt ctgtgaaagc
     1681 ttttgaagtt ggaccgtcta gtgaaatatc gtttgaatcg tataaaacga tcaatttccc
     1741 tagtttcata tgacctgcca tcgaacttgc ttcttgagat accccttcca tcaagtctcc
     1801 gtcaccgcat attgcatacg tatagtgatc catcacatta aagttttctt tgttatacgt
     1861 tgctgcaagg tgcgcttctg ccattgccat accgacagcc attgcaatcc cttgtcctaa
     1921 tggccctgta gtggcttcta caccatctgt atgatgtact tcaggatgtc ctggtgtttt
     1981 agaatcccat tgacggaatt gtttcaaatc atcgattgtc acttgatagc cagctaaatg
     2041 aagcaagcta tacaacattg ctgacccatg tcctgccgaa agaacgaaac ggtcgcggtc
     2101 agcccagttt tttgatgtcg ttggatttac tttcaggtgt tttgtccata acgcatatgc
     2161 cataggtgct gcgcccatcg gcaatccagg atgtcctgag tttgcttttt ggactgcttc
     2221 aatacttaat gtacgaattg tgttaacgcc aagttggtca attttgtcaa acaaaataac
     2281 ccctcctatt aatttaggtt ttttttacct ttatttctta cactttcatt ctataagatg
     2341 tcacacgaaa aagcaatctt tttcagtatt ttttactaaa agttgtcatt tcggttgtgt
     2401 aagccttttt tcttttggat ttcttttaat ttttccgggg tcacgtcatt gccctcagga
     2461 tcgacgactt tcattccttc gatatgatga cgcataccgg aacgaaaagc ttccaaatac
     2521 tctttgcgca agttgatttg ttcttgttct tcggcttttg agagaccatt ttccttcttt
     2581 ttacgtgcaa gttcattgat ccgctctatt ttttcaggtg acagcatgct cagccctcct
     2641 tttactctcc ttcatattat ataaaaactg aaaaaaaaac aactagataa actaaataaa
     2701 atacgaacat tcgtttgatt tagcgctttt tatatggtac tatttattta tcaagtgaaa
     2761 gaaggtgtac cttgtggccc gacaaactga atcaagacaa atcgaagtct tacgatatat
     2821 ccatgaacaa gtaacccaaa aaggataccc gccgactgtc cgtgaaatcg gggaagctgt
     2881 tcagctttct tctacttcta ctgttcacgg tcatcttgca cggcttgaaa aaaaagggtt
     2941 tatccaaaga gatccgacga aacctcgtgc aatcgagttg acaaaacaag gtttagaaaa
     3001 gattgggatt cgtccaacaa ctattccagt acttggcgtc gtaacagctg gagaacccat
     3061 tttagcagta gaggatgctt cagacttctt tccaatccct cctaatcttt cttccgaaga
     3121 aggcagtctc tttatgctga caattcgtgg agatagtatg atcaatgctg ggatattgga
     3181 tggtgacaac gtgatcgttc ggaagcaaga aactgctcaa aatggcgata ttgtcattgc
     3241 aatgactgcc gaggacgaag cgacttgcaa acgattctat aaagaaaaaa actttttccg
     3301 tctgcagcct gaaaacgact tgctcgaacc aatcatctta gatcaagtat ctatattagg
     3361 acgagtagta ggactttatc gtagtcatat ctattaattg ctactaatta aaagaaaact
     3421 ttttaaaaag agtttgagac gttggtctca aactcttttt tcgcccgggt tcttcttctt
     3481 tcaaattttt atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg
     3541 aacaattgtt cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc
     3601 ttagtattaa ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct
     3661 ttacttgtgt ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac
     3721 cgtcaatctg agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga
     3781 tccaggcaat cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt
     3841 tacgggggtg tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc
     3901 cagtttttcg gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct
     3961 ttgcttgttt ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact
     4021 tttgagggtc gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc
     4081 tgcttaatat tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag
     4141 atccatcaat tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag
     4201 ttcgatcgtc gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg
     4261 ttgacctggt ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc
     4321 ataagtcact tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc
     4381 gactcctaac tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac
     4441 taccatatca attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata
     4501 agcgtcacca tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata
     4561 gtcgggaata ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta
     4621 accatttctt tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat
     4681 tgagccgtcg ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt
     4741 tttgtaaaac accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa
     4801 aaattaataa tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta
     4861 tatcaattcg ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc
     4921 ctaccccatt caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg
     4981 aataagaccg aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta
     5041 gagagtttgg ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa
     5101 tcgcagcttc ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat
     5161 aaaggctacc ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata
     5221 cttgcagacc acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc
     5281 attcattgat cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt
     5341 gagtaccaaa accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt
     5401 tcagatgctg ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata
     5461 aaaactgagg caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc
     5521 gctctactgt tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca
     5581 tcaagtcaat ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat
     5641 tttcacttgg catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat
     5701 ctcgcatctg gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt
     5761 tagctgatcc atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta
     5821 ttttcccagc aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga
     5881 tatgattctc cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt
     5941 ttctttcttc attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa
     6001 acgtcgcaag taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc
     6061 tgggagcaat cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt
     6121 cttcaaatag cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg
     6181 aaacaaggat tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta
     6241 ctaaatccaa aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta
     6301 ctccgactcc tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa
     6361 tcgcataagc gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta
     6421 caccagcttg ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga
     6481 tgatcaactg gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc
     6541 catgctcaaa tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt
     6601 ctttcattac cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt
     6661 tttctccact gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca
     6721 gatacggatc atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct
     6781 catggtaacc tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga
     6841 tcatttggag aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt
     6901 gtctcacttt ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca
     6961 gaaagaaaga ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt
     7021 ttgtacgacc atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg
     7081 attgagtttt ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta
     7141 actccgcctg ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt
     7201 cttcttttgc ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag
     7261 tcagctgttt gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa
     7321 tcagtgcacc aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag
     7381 tgaagccaaa acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca
     7441 aaactaaaac aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt
     7501 tgttaaaaat tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg
     7561 atgcaacttc taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga
     7621 cgatcatttt tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc
     7681 ttccgcttgg atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg
     7741 taacagctac gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg
     7801 caaatttcgt ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg
     7861 cgatattaat gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca
     7921 aaaccatcat tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg
     7981 taaggaagtc ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga
     8041 cttcttccat caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct
     8101 tgaaagcata agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa
     8161 atgccattct tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga
     8221 tttgtgcgcc cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca
     8281 tgcctcttcc tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt
     8341 gttttcaaac acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct
     8401 cgcactgata tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc
     8461 ttgatgagtc gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt
     8521 gctttcatgt accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct
     8581 cgtatcgcct aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc
     8641 attgatcgtc cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag
     8701 tggtccagct ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc
     8761 atgttcaacc acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac
     8821 agaaaattgc tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg
     8881 ataacttaaa cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt
     8941 tggcccgtaa atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc
     9001 gggaagtcca aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct
     9061 cggacgaata gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca
     9121 aacttcattg cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc
     9181 aggaacacct gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa
     9241 gttcctcttt aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt
     9301 caaattaaaa ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag
     9361 cagcgaaccc gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca
     9421 ttgtcgttgg aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca
     9481 tgattttcag cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga
     9541 gtaatttctc cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca
     9601 tcagcaatca agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac
     9661 acggtctggg catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca
     9721 aaccatccta ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat
     9781 ccacccgtag aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga
     9841 tgtgtcccat taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca
     9901 gcatgtaact ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt
     9961 aaaatgatta ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt
    10021 tacctttaat tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg
    10081 tgacaaaagt cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac
    10141 ttctgtcaca acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct
    10201 tgcctattcg acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc
    10261 accgcgtgcc cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat
    10321 cactgtttca tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga
    10381 taagatccac gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa
    10441 tccatagtgt acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac
    10501 ttctaacaaa tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga
    10561 aattggaaat actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc
    10621 tttgaatttc actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat
    10681 ttgcggacgc tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata
    10741 gtcttcataa ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg
    10801 tgttctttcg atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc
    10861 aatcaagcct ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc
    10921 tagattaggt acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat
    10981 aacggatagt aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa
    11041 tactttcaat tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc
    11101 cggtgcagga ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt
    11161 tgccacaact agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc
    11221 atctcggaca gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg
    11281 catcccttta ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa
    11341 atccatcaat gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc
    11401 gtctccgcca gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc
    11461 gtctccgccc ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat
    11521 taagtcctcc tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag
    11581 tcttacttat tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt
    11641 accttcttct ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata
    11701 attttttgac aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga
    11761 ggatgccgtt tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag
    11821 gtattaacgg atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag
    11881 atgatattga agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat
    11941 tgcaatttga ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa
    12001 tcgtagtgga tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt
    12061 gggcaaaaga aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag
    12121 aaaaagcaaa agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg
    12181 gtgctatgaa aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa
    12241 tcatctcagc aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata
    12301 atgaatttgg gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa
    12361 tgctcttaga tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca
    12421 atactatccc acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac
    12481 aaggaaaatt acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat
    12541 tggtttcagt attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac
    12601 acacagttga caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca
    12661 ttggaacaac tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg
    12721 atttccaact tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa
    12781 tgattcgctt actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa
    12841 gcctttgact ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt
    12901 caatgaacat attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc
    12961 ttgtatgctt ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt
    13021 gcacgataga ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata
    13081 aagagcattg ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag
    13141 tagccagcac ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga
    13201 ctaaacatat cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct
    13261 tcaatgattg ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct
    13321 gctcccatgc tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact
    13381 ttgtttatcc actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct
    13441 tcgctttttc catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac
    13501 atctttgcgt aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct
    13561 gttttttcac ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt
    13621 gaagtgatgg tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt
    13681 tctttggatg tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc
    13741 acaacagcat aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa
    13801 gcgacgccta aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt
    13861 ttcatataac cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat
    13921 aacccgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    13981 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    14041 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    14101 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    14161 aggcaagact gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtggtag
    14221 aaaagtcatt caattacctg ctcatgaagt agattacatt aaagagaaag tcactcttgg
    14281 ttggacgcct gacgtcatta tcgggcgaaa agaaagg
//
LOCUS       DAPBCS010000065        14283 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPBCS010000065 DAPBCS010000000
VERSION     DAPBCS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.067"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="RD521_002442"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="RD521_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDT7772122.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="RD521_002443"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="RD521_002443"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDT7772123.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="RD521_002444"
     CDS             complement(2169..2906)
                     /locus_tag="RD521_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7772124.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(3003..3524)
                     /locus_tag="RD521_002445"
     CDS             complement(3003..3524)
                     /locus_tag="RD521_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772125.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="RD521_002446"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="RD521_002446"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDT7772126.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="RD521_002447"
     CDS             complement(4401..4886)
                     /locus_tag="RD521_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDT7772127.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="RD521_002448"
     CDS             complement(5039..5236)
                     /locus_tag="RD521_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772128.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="RD521_002449"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="RD521_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDT7772129.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="RD521_002450"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="RD521_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDT7772130.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="RD521_002451"
     CDS             9211..9423
                     /locus_tag="RD521_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDT7772131.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="RD521_002452"
     CDS             9425..11104
                     /locus_tag="RD521_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDT7772132.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="RD521_002453"
     CDS             complement(11358..11558)
                     /locus_tag="RD521_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDT7772133.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="RD521_002454"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="RD521_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="RD521_002455"
     tRNA            complement(12249..12322)
                     /locus_tag="RD521_002455"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12634..13032)
                     /locus_tag="RD521_002456"
     CDS             complement(12634..13032)
                     /locus_tag="RD521_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772134.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            complement(13025..13477)
                     /locus_tag="RD521_002457"
     CDS             complement(13025..13477)
                     /locus_tag="RD521_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772135.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            complement(13464..13871)
                     /locus_tag="RD521_002458"
     CDS             complement(13464..13871)
                     /locus_tag="RD521_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772136.1"
                     /translation="MSFLLLKYLEIYSVKKNKLIGENDSPIKTPIELSLRNRMGILQR
                     SEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDATATTALTFGFTKNGTINSDK
                     TRQAATESDGICKKVCNGEENYWIGEEKLNEIY"
     gene            13850..14152
                     /locus_tag="RD521_002459"
     CDS             13850..14152
                     /locus_tag="RD521_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772137.1"
                     /translation="MLIEGKTQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         4227 a         2977 c         2411 g         4668 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagcaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc aacaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 cttcaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaacta agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatacagt
    12481 catctaaata cttcatatca atcatttatt cagaaacaaa ctatcttgca ggatctatac
    12541 gagttaatta cagagcaaaa aagcctcaac caaggatgac tggttgaggt ctttgtcttg
    12601 caaatttaaa tagataactc tattacttgt attttatttt tgttgactat atagctgata
    12661 taaatcaata actgtaggca taactcctac ttcgctatat aaattagaaa tcaaattaga
    12721 agctttattc aggataggtt tagctagctc tcccatagat tcttctacta attttttata
    12781 tggctgttca tttgtagaaa cacgaataac tgaaaagagt aaaatatcta ccggtgctcc
    12841 ttccatcccc cctacaatgt gagctcgtaa agaaacaagt tttttttcta gatactctgc
    12901 ttcgtcttct ttatatccaa gttcaatatt aaactctaaa tctccataac taaaattatc
    12961 agttaattca aaatgaattt tattcaaact aatattttca agtgcaaaag taacactatt
    13021 tcctttatcc atgtacatat acctcttttt ctacaatata gcggctcgta tcttggtaat
    13081 taacactagc atagaccata tttgacgata cttcttttct ctttctactt ttaaatactg
    13141 agtaactttt attccgaata taaggtttat tttcaatacg gtcatattta atagggccgc
    13201 cagcaacctt aatagtgaaa ttcattgaat ctttatagga caaattttca acttgtttca
    13261 aaagtttatt gtactctgca acaggaatat gcatatctcc gaactcaaat tcaagatatt
    13321 ttttttcact tattcctaac atttttgaga attctgtcac gtttttttgt ttttttaatc
    13381 ttattttaag taattgtttg gacaggtttc tttgttcaga atttaatttt ttatataaat
    13441 caggatgttt ttttaataaa tcattaatag atttcattca acttttcctc ccctatccag
    13501 taattctctt ccccattgca tactttctta catataccat cactttctgt agcagcttga
    13561 cgggttttat ccgaattgat tgttccattt ttcgtaaaac cgaatgttaa tgcagtagtc
    13621 gcagtagcat cttctataga attgtatacg aaaaaaacaa ttctataatg ctcataagta
    13681 tcatctagtc ttatttcata actaaattta tgataaaaaa gttcttttac tatttccgat
    13741 ctttggagta ttcccatccg atttcttaag cttagctcaa taggtgtttt tattggagaa
    13801 tcattttcac caattaattt atttttttta acagagtaaa tttcaagata ttttaataga
    13861 aggaaagaca caattatggt ttaaatttga tccttcgaat agatttatca aagattttta
    13921 taaggtgtgg gattcagaag ttttctttct agcaatcgaa gatagcttat taatcaatct
    13981 ctactattct aataagaact actttaaaat ccctgctgca aaaactagaa tgaagaaaga
    14041 tgtatacttt ttgtttgata tcgtgactga cgtgccggac gcacgtagcg atcatcggcg
    14101 ttatgactat ataaagtata ctttcgttga tccagaaagg tataaagatt aaagtaggct
    14161 acctaaaaag gtagcccgga acgaatttta tcaccatact tgtgaaagga gatatttttt
    14221 aagttagtat taagattgtg taatatgatg atatctatat tttatagtat cagtgctata
    14281 aaa
//
LOCUS       DAPBCS010000066        14033 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPBCS010000066 DAPBCS010000000
VERSION     DAPBCS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.068"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(63..1268)
                     /locus_tag="RD521_002460"
     CDS             complement(63..1268)
                     /locus_tag="RD521_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7772138.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="RD521_002461"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="RD521_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="RD521_002462"
     CDS             complement(1447..2832)
                     /locus_tag="RD521_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDT7772139.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="RD521_002463"
     CDS             complement(2861..3247)
                     /locus_tag="RD521_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7772140.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="RD521_002464"
     CDS             complement(3263..3577)
                     /locus_tag="RD521_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDT7772141.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="RD521_002465"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="RD521_002465"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDT7772142.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="RD521_002466"
     CDS             4837..6126
                     /locus_tag="RD521_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDT7772143.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="RD521_002467"
     CDS             complement(6165..6770)
                     /locus_tag="RD521_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDT7772144.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(6975..7439)
                     /locus_tag="RD521_002468"
     CDS             complement(6975..7439)
                     /locus_tag="RD521_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7772145.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7461..7781)
                     /locus_tag="RD521_002469"
     CDS             complement(7461..7781)
                     /locus_tag="RD521_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDT7772146.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(7925..9004)
                     /gene="pepA"
                     /locus_tag="RD521_002470"
     CDS             complement(7925..9004)
                     /gene="pepA"
                     /locus_tag="RD521_002470"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDT7772147.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9204..9554
                     /locus_tag="RD521_002471"
     CDS             9204..9554
                     /locus_tag="RD521_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDT7772148.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            9777..10724
                     /locus_tag="RD521_002472"
     CDS             9777..10724
                     /locus_tag="RD521_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDT7772149.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(10781..12199)
                     /gene="pepV"
                     /locus_tag="RD521_002473"
     CDS             complement(10781..12199)
                     /gene="pepV"
                     /locus_tag="RD521_002473"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDT7772150.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            12348..13193
                     /locus_tag="RD521_002474"
     CDS             12348..13193
                     /locus_tag="RD521_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDT7772151.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            13197..13637
                     /locus_tag="RD521_002475"
     CDS             13197..13637
                     /locus_tag="RD521_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDT7772152.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            13687..13881
                     /locus_tag="RD521_002476"
     CDS             13687..13881
                     /locus_tag="RD521_002476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772153.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
BASE COUNT         4070 a         2843 c         2627 g         4493 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaaga tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcag aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg actattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtgatct acaaaaaaga cgggaatact gcttatgtag tcacgaataa tcacgtagta
     5281 gatggccagc aaggtcttga agtcttgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcttattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acagttgcct cttttggtga ctcaagtgct ctgaaggttg gtgagcctgc gattgcgatt
     5461 ggttccccac taggatctga atatgcaaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag tgacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag caatcaaccc tggaaattct ggtggtcctt tagttaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatctacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc tattccaagt aacgatgtag tgaatatcat caatcaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gctgaaaaag ctggattgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatgct
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt tttttgctgg agcatttggc
     6241 aaatgcaatt cacgagcgga acagatcatt ccatagctat ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcata gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tattgggtgc accgcaaacg atttgcaata ctgtaccatt gtctacttct
     6421 gtctcagtaa tcgataaatg gtcagaatca ggatgtggga cgcatgtttt tacgtacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttgatc
     6541 aatcgattca atttcgctaa ttcctcatct gttaagctta cttggccgtt cccttcgatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt gcgttcaaca gtgttttctg ttcctttatc gtcggctata
     6721 ataaccatca aggtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgaaattttt
     6841 ttcatgcttt atttccgaag aagcctcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgttttagtt aattgattaa aaaggtcgta
     6961 caagttccac ttgcttaatt acttggctgt tcttcttcgt cagaaacgat caagacatca
     7021 caaggtgaat gacgtatcac gtagctggct acacttcctg tcataaagcg ttcgactgca
     7081 tttagacctg actgcccaac catgattaga tcgatctcat atttttttgg caatttgact
     7141 gccatggatt cacgaacaga accatatgca ataattcctt ctatatttgt gtaatcaaca
     7201 gatttcccat attctttaca ttcttcgatc atttccttgg ctgtttcagt ttgctgatcg
     7261 actagatttt gatcaatagg tgcatagccc ataaagttat aaaattgctg atcgattacg
     7321 tttgctacat atactttccc attattacgg cgagccactt ctaccgcttt tttgaaagca
     7381 gcaagtgctt gttcactacc gtcgatacct actaaaatat tcttatattc ttgattcatc
     7441 ttgacccctc ctaacagatg tcagcctttt gttacacttt ctaggaacga ttcgatttcc
     7501 tgttttgttt tacgatcttt attcactaga cgacctaatt cttttccgtc ttggatcacg
     7561 acaaaacttg ggataccaaa aatgttccat tcagcggcta catctatgta cttgtcacgg
     7621 tctacttcaa taaattgcca atcagaaaag tcattttcaa tttcaggcat ttgcggtttg
     7681 ataaaacggc agtctccgca ccagccggca gtaaagaaaa atacactctt tccagtttct
     7741 acgtagcctg ctaattcttc taaactattt gggataatca ttctttgtcc tctactttct
     7801 gtttatgtat ttttcaatta aattatacac ctttctttcg aactaacaaa cattgatcct
     7861 ctttttataa aaaaaatctg gcaaaaagca atcttttgcc agattttata gtttcactat
     7921 cgcattattt tccgtatacg atcgtattta ctgttgattt atccaaacct ttcagtgttt
     7981 gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat
     8041 ggatataacg tcccactacg ccaatcactg tacttgggat tccttcgttt tgtgtatgag
     8101 cagcacctgc atctgtaccg ccttttgaaa caaagtattg ataagggata ttgtttgttt
     8161 cagcgatatc cagcaagtac tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga
     8221 tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat
     8281 ctgctgcaga gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt
     8341 tagaaccacg caagcctact tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta
     8401 atgtttcatt ttgcaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt
     8461 cccatgattt actgataata ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa
     8521 cgattgtgtc tcctggaagg acaccgtaac tcattgcctc ttctcttgat tcaaacccag
     8581 catcgaacaa aatatcggtt acttctactg atttttgtcc gcttgttcct cgcaataaat
     8641 gtgggggaac ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa
     8701 aacgctgagc agaaacaacg taaggattcc agccgcctag aggtacgact ttaaaaagcc
     8761 cgttttcttg gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta
     8821 cacggggtgc atccgcttct tcatgatggc gcagaccaaa aatgccgccc aaaccatcta
     8881 actgtaactc atctaccagc ggttccatat gactttccat ataagcgcga acatcttgtt
     8941 caaaaccgct tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt
     9001 ccatatgcta ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg
     9061 ataaaatatg attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag
     9121 cggttattta tttcaaagaa aatatgagag tgtggtaaaa taagcacaag gagtaacaag
     9181 tgagaagaag gaggtatgtc cacatgtatg aagaaagcga actaagctat tttaaaggcg
     9241 ggttagcagt tggaatcggt ctaggattat tgagcggggt agcttcaacg atttggtatc
     9301 atcgcaaaaa agtattgtca gcagaccaag tacttgaaaa tatcaagtca tcttttctaa
     9361 aagaaggtgc aatcgaaggt tcttggatct cttttgaaaa ggaacctatt aggaaattcg
     9421 ccgttcggtc gagtgcttat cggggaggtc tttctcgaat agaagatggc gaaactgttt
     9481 attatgaatt tttagcagat gctcatacag gaaccgtatt agaaatttca agaaaaaaag
     9541 gtgcaacaga ttaatacgtc aaaaaagtga gagaacgaat acgcacccac tattcaaaag
     9601 agaggtatca tgtccttctt ttgtagtggg tggtttttta gatagagctt tccaaacata
     9661 tattccagta ttttcttttt aaataaatga tatttagcgg gaaaaactag catttccttt
     9721 gattatgagg tatttttaat ttagcttttg ttttctatag ttaaggagag tgaaaaatgg
     9781 aacaacaaga aaaaattgct gttctgattc cttgttacaa tgaggaagca acaatttcaa
     9841 cagtcattgc agactttaaa cgagaattac cagaagcaga tatttatgtc tatgataata
     9901 attcgaccga tcgaacatac gaattggccg ttcaaggtgg agcaatcgta aaaaaagaac
     9961 ctcgtcaagg aaaaggaaat gtgatccgcc aaatgttctt tgatatcgat gccgactatt
    10021 atttaatggt agatggcgat gatacttatc ctgcagaagc tgtacatggt ttgctcgaga
    10081 aattacgttc tggtgaagca gatatggtca ttggtgatcg cctatcaaat ggaacctact
    10141 ttgatgagaa taagagacct ttccacgatt ttggaaataa ccttgttaga aatacaatta
    10201 ctagaatgta taaaacaaaa attcttgatg tcatgaccgg ctatcgagga ttcaatcgta
    10261 tctttgtcaa aagttttcct attatgagtt ctggttttca aatcgaaacg gaattgacga
    10321 tccatgcttt agataaaaaa ttcaagttcg ttgaaatccc aatcgattat cgtgatcgtc
    10381 ctgaaggcag cgaatcaaaa ttgaatacat tctctgacgg attcaaagtg atcatgatga
    10441 ttattaaaat gtttaaagat tataagcctt tgcttttctt tgggatttgg accatcttct
    10501 tctttttatt tggattggtc acggggattc ctgtgattcg cgaatatatg ctgacaagct
    10561 ttattacacg catcccttct gcgattttaa gtactggatt gatgacttta gcacttcttt
    10621 cattagtaac agggttgatt ttggatacag tcgtcacgaa cgctaagaaa gaatatgaat
    10681 tgaatctata tcatgtttat catgaatacc acagtagaaa gtaaaaaaag gatcaagact
    10741 gcaaaaataa tttgcaattc ttgatcctct ttttaattaa ttatttcgcc aaacgataga
    10801 tggcatctgc atagattgct gttgctctga acaagtcatc aagactcata aattcgtttg
    10861 cttgatgcat tgtatcagta tatcctggga acatcgctcc ataagcaacg ccacgtttca
    10921 acaaacgtcc gtaagttccg ccaccgatga tttgttcgta gcctttttct ccagtgtggt
    10981 cttcatagac ttgcaataat gtagccacca atggatcatc cattggtacg taatgtggtt
    11041 ccattacgcg agcacctcga gtaactgttg cgccttcttg ccccactgtt tgttcgatgt
    11101 cactttgcaa tccgtctact gttacacctt ttggaaaacg gaaattcata ttgatgaagt
    11161 tcgcttcctc tgtaccattt tcttcaaaag caaatagtcc agcattcatt gtcaatttgc
    11221 ccattttttc atcttcataa gcgactccta gtttttctcc ataaaaatct tcatggacat
    11281 aagcagctgc tacatggata aattgtttag cagatcctag gaattcatag ttatctaaga
    11341 acaatgccaa gaaagaacct gcattgatac cagattgtgg gcttgcacca tgtgcgcctt
    11401 taccaactaa ttcgattttt acatacgtat tgtccgcttc aatcgttccg cttactggat
    11461 ttgcttcgat gaattggtaa aatgcttctt ccatcgcgat agctgcatct gcacttggta
    11521 cttgaagtgc tgcagttgct gttcctggca ccatattttc gcgaagtccg gaaacaaaag
    11581 atttcaagac ataatctcct gcattgtcgc ctttgaagcg taaagctaaa gaaacatttc
    11641 ctttttcacc attgatgatt gggaattcag catctggtga gaaaccaaaa tctggttctt
    11701 cttcatgttc gaaataataa gccatatcgc cccagccgct ttcttcatcg ctgccgacta
    11761 cgaagcgaac tttttttgat agtttcaagt caagttcttt aatgatcttc atcgcatagt
    11821 aagctgccat acttggacct ttgtcatcgc ttgatccgcg tgcaaagatc tttccatcct
    11881 tgattactgg ttcataagga tctgtgtccc agccatctcc tgcaggaacg acatccatgt
    11941 gtccaaatat ccctagcgtt tcgtctcctt cgccaagatc aatatgccct gcatagttat
    12001 cgacattttt tactttaaat ccatcacgtt cgccataagc taacatatgt ttcaatgcgt
    12061 cgcgaggtcc aggtccaaat ggtgcatctg gtgttacttt tgagtcatcg cgctcgctgt
    12121 tgacacgtaa aagatttttc aagtcttcaa gcaagtcttc tttacgtgct tctacttctt
    12181 tttgccaatc aattgtcata aagttaatcc tccacttatt taagtcttcg tgttcatgat
    12241 accacaaatt acagaaaaaa atagttcaaa aaatctgaca attccttttc ttatctgttt
    12301 ttctctaaaa aaaatatacg ctataatgag taaaaggagg tttttagatg ctgataaacg
    12361 aacaactagt gaaaagagtc atcacacctc gtccaaaaat ttcacataaa ggaacatttg
    12421 gacgtatcgt tttgattggc ggcaatgaac aatatggcgg agcaatcatt atgagtgctt
    12481 tagctgccgt acacagcgga gcgggtctca cgactgtagt aactgatgaa aaaaatcgga
    12541 gtgctcttca cagtcatctg ccagaagcaa tgattgtagt ttggacggat actgaccagt
    12601 ttctttccgt aataaagcaa gcagatgtac tcttgattgg tcccggattg ggtacttctt
    12661 ctaagagttt ggaacgtttc aaacaagtac tagaaaacca atcaaaagaa caatggctgg
    12721 tgattgatgg ctctgcttta agtttacttg ctgctaatca tcttttgcta ccctatcccg
    12781 aacaaactgt acttacacct catcaaatgg agtggcaacg gttgagttct ctaccgatac
    12841 aagaacaacg ggataaagaa aacaaaaaga agcaacagga aatcggtgct gtgctcgttt
    12901 tgaaaagtca tgagacaaaa atttatggtc accagcactt ctacaccaat cctttaggtt
    12961 ctgctgctat ggcaacgggc ggcatgggtg acactttagc tgggatgatc acaggatttt
    13021 tagcccagtt tcctcaaaat gaccaaacga ttgcagcagc tatttatctt catagtttca
    13081 ttggagacca gctggcaaaa gaacaatata ttgttttgcc gacacaaatc agcgaagcac
    13141 ttccgaaatg gatgaattat ttttgtaaaa aatcaatgga aaacgaggca tgagtcatgg
    13201 agaaatttac aggttatgtg cgagaacctt tttattatga aacagatcaa atggggatta
    13261 ttcatcacag caattatatc cgctggtttg aagaggctcg ggtagcctta ttggtacatc
    13321 tagggtttcc ctataaaaaa atcgaagaat ctgggatcat catccccgtt ctggaagtaa
    13381 attgcagcta taaagaaatg atccattacg gagaaaaagt gagcatccat ccaactatcc
    13441 aaaagtatac ggggacacgg ttggatttta gctacgaaat tgtgggaact gaagacaaaa
    13501 aattaaaaac tactggcagt agtaaacact gttttttact ttctgattcg caacgattag
    13561 tgaagctgag caaagtcaat ccggagttag atcagttatt ccaagcgtac gcttctttgg
    13621 atgatgtaaa ttcttaataa agaccgtcta agcatttttc ttgtattgtg tcgagtagtt
    13681 cgtataatga aaagaaaaaa gcaggtgatc cccatgtatg tcgttaaagt aatgcacggt
    13741 tacatcgata aaactggctg tcgcactcgg gaaaaaaact tagacaatct gctcattttc
    13801 aaagacaaaa aagaatccga agcttttgct aaaagaatcg gcggtcgtgt caaaccgatc
    13861 caagaggtac gaccagattg attcaatatt tttcttttcg aggctctaat aggcatgcct
    13921 atcagggctt tttttgcaga ctgtgttttt atgcccacac gatttgtaag cgatttatgt
    13981 tataatagat tatatctgca tagaaaggaa ggttaaatgg ttatcaataa ttt
//
LOCUS       DAPBCS010000067        13899 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPBCS010000067 DAPBCS010000000
VERSION     DAPBCS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.069"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            149..1600
                     /locus_tag="RD521_002477"
     CDS             149..1600
                     /locus_tag="RD521_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDT7772154.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1949..2581
                     /locus_tag="RD521_002478"
     CDS             1949..2581
                     /locus_tag="RD521_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDT7772155.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3169..4608
                     /locus_tag="RD521_002479"
     CDS             3169..4608
                     /locus_tag="RD521_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7772156.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4903..5121
                     /locus_tag="RD521_002480"
                     /pseudo
     CDS             4903..5121
                     /locus_tag="RD521_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5370..5585
                     /locus_tag="RD521_002481"
     CDS             5370..5585
                     /locus_tag="RD521_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772157.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5802..6058)
                     /locus_tag="RD521_002482"
                     /pseudo
     CDS             complement(5802..6058)
                     /locus_tag="RD521_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6245..7156)
                     /locus_tag="RD521_002483"
     CDS             complement(6245..7156)
                     /locus_tag="RD521_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7772158.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7175..8179)
                     /locus_tag="RD521_002484"
     CDS             complement(7175..8179)
                     /locus_tag="RD521_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7772159.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8176..10299)
                     /locus_tag="RD521_002485"
     CDS             complement(8176..10299)
                     /locus_tag="RD521_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDT7772160.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10304..12751)
                     /locus_tag="RD521_002486"
     CDS             complement(10304..12751)
                     /locus_tag="RD521_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7772161.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12738..13127)
                     /locus_tag="RD521_002487"
     CDS             complement(12738..13127)
                     /locus_tag="RD521_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7772162.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13187..13690)
                     /locus_tag="RD521_002488"
     CDS             complement(13187..13690)
                     /locus_tag="RD521_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7772163.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13703..13864)
                     /locus_tag="RD521_002489"
     CDS             complement(13703..13864)
                     /locus_tag="RD521_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772164.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4301 a         2728 c         2531 g         4339 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcatca agtgaaagta gtacgacttc aaatgccgag tcttcaacaa cttcgtcaag
      481 tgaaagtagt acgatttcaa gtaccgaatc ttcaacaact tcaagtagta caagtgaaat
      541 aacttcttcg aaagtgacaa aaccaaacca tatagaatct tcaaaaaagg aagcgccaaa
      601 aacggaagga caaacaaaaa ctttttcgac accacagcaa atagaatcaa cagaatcttt
      661 ccaatcacaa ccaatactat ctggtaatga agaaattcat tttgacaaaa atgaatctgt
      721 agaaaatttt attatgaaaa ttggtgagtc tgcgagaaaa attggtcaga aatatgattt
      781 atatgcttct gttatgattg cacaagcgat cttagaatct gccagtggtc aaagccagtt
      841 agctcaggca ccgaattaca atttatttgg gataaaagga acttataatg gaaactttgt
      901 cattatggta acaaacgaag atttgggtaa tggaacgctt tatacgaccc aatcaaaatt
      961 tagagtgtat gaaaactatg aggaaagctt tgaagattat gccaaattat taactaaggg
     1021 aattagtgga aataaagatt tttatgcagg agcattaaaa gctaatagta aaacatatcg
     1081 agaagcaact aaattcctta caggacggta tgcaacagat acacaatact atttgaagct
     1141 aaatgaatta attaaaacct acgatttaac caactatgat aaagaagtta aaggtccaca
     1201 aatgagtaag actggatacc aagtgcctat tcataattat tcaataacca gtttgtttgg
     1261 aaatcgtgga aatgaatttc accgaggact tgatatggca gctacacagg gtgagccgat
     1321 tcatgcagtt aaagctggta aggttgttaa agcagagttt cactactctt ggggaaatta
     1381 cgtggtgata caacacgaag atggatcaac cgcgttatat gctcaccagc aacaatacct
     1441 agtaaaaact ggagataatg ttttgcaagg acaaataatt ggctatgtag gaagtactgg
     1501 gaatagtact ggcccacacc ttcatttgga aatttgcaaa gatagtagtc tttctcagag
     1561 catgttagtt gatccgaaaa ccttattatt cggagactaa aaaaggtaat caatctgaca
     1621 aagtaacaag aaaatggttg gattcttaga cctttgatca ggatattgag actggaattg
     1681 atatctcgat tacgatgggt ttgacaaata aaacaagtcc attatcgaat atcgagtgta
     1741 ttattgccgt ctttatgctc atattttgag caggtttcac tagatgaaaa caatttatct
     1801 atcttgagga tttcgtgtac gtatcgttca gtcttgtatt gtaaattagt cataaaatca
     1861 gactaagaaa aattggaaat atttttttct gatttattaa aagttaattg aaattatatg
     1921 gtacttcttt cttgggaggg taggtgcagt gaaggaaaga tatggtcacc ttactgttgt
     1981 tgaggagatt actcataatg gatataaaaa atatcgttgc ctatgtgact gtgggaatga
     2041 aacgattgta tatcgatcaa acttactaac aggtagaacc aaaagttgtg gatgtggaaa
     2101 ttataaaaat aggcataaat ataaaaattt gacaaataaa acttttggta agttagttgc
     2161 aatcaagcca actaaactgc gaagtgaagg aactgttgtt tgggaatgtc aatgtctgtg
     2221 tgggagaaaa aagttagtat cggcaaagcg acttctaaaa ggtgatgtaa aaagctgtgg
     2281 atgtttgaaa tcaaaaaagc atcacattga aaataaaaaa ttcggattac ttactgcaaa
     2341 gtatcctttg gacaataact gttcatggaa aacaaaatgg gtttgtcatt gtgagtgtgg
     2401 cagtatgtgt gagataagtt atagtaactt attttttgga catactcaaa gttgtggatg
     2461 tttaaaaagg caagaaacaa ggacatgtgt tgaagggaca gtaatagagt gtctaacgtc
     2521 aaaattaccg aaaaataata caagtggtgt taagggagtt tatcgttcta aagagaaata
     2581 gattgcgtat ataactttaa aaaaaaagag gtactattta ggatcgtatg attctattaa
     2641 gagtgcagct caagctagaa aagatgctga aaataaattg tttcaacctt tgattgacaa
     2701 atatcgaggt aatcattaat tattgtggat aataattaac agtgatatat aaacatctat
     2761 aatatattat tcatactatt gatatttagt ataatcctat ggaattgaat atcatagcaa
     2821 tgtatttgaa aagcaaattg gaaaacaaat aggtgcaaca ccataaatat gaaaaaattg
     2881 ggaaaaagta gaatggaaat ttgcttacgt ctcacgagat agctcacact attaatctga
     2941 agaacaatga agtcgacttt tagaaattgc tagaagaaag taaagcgaat tatcactagt
     3001 tactatagag taagttcaaa taaagcatct ccaacttaca tggttacatg aggatatcaa
     3061 atcgaaatta ctcaagcttc ctatggctag agtcgcgaga agttgttggg cagtagacat
     3121 aggttatatt gttcttagct acacgatcat tagaaaggaa atcctattat gtatagttat
     3181 caagaagatg caaaagtcca aaaaaatcgc tggtggattt tgattgctat atctatgttt
     3241 accttcatgt ccacattgga tggcagtatt gtcaatattg cactacctac catctcagat
     3301 gatatgggca taccaatgaa tcaagcggaa tgggtagtgt ccgtttatct gatgattgtc
     3361 tgttcttgtt tgttgctgtt tggtaaaatt ggcgatagcc gtgggaaaat caaagtattt
     3421 cggattggta cgctgatctt tgtcttggga tcgctgttgt gtgggttcaa tcattcgcta
     3481 ggtattttat tgttcgcacg ggtggtacaa gccattggtg ctagtatgac tatggctaca
     3541 aatgccggaa tcatcacgga agtctttccc atacatgaac gtggtcgggc attgggagca
     3601 ataggtgcgt ttgtttcatt aggctcgatt gctggaccag gtcttggcgg cttgattttg
     3661 tcccaatttt cttgggcgta cattttttgg atcaatgtac cggtgggaat cgtaacgatg
     3721 ttgattgctg aaaaattctt accaaaagat atcaccatga gcggccatac aatcgatgga
     3781 gggggctttg ctgccattgc ggctacgatt atcagctttt ttggagcaat ctttttgggg
     3841 caagaaagtg gctttttcca tccagtcccc cttgtactgt tcgtaatttc agtgattgcc
     3901 tttgtcagct ttttaaaagt tgaaaagaaa gcagcctctc cattgatttc tttcggtatt
     3961 tttaagaaca agatttttac catgagcttg attacagcct tgttgatttt ttcttccaac
     4021 ttctttgtca atgtagtgat tcctttttat ctgcaaaatg ctcgcgggtt gagtcctagc
     4081 tatgctgggc tgttgatgat ggtctttcct ttgatgatgg tcgtcggggc gcctttaagc
     4141 ggctacctga ccgaccgggt tggcccggag attttggtat tagtaggctt gggcttattg
     4201 tcggtaaccc aggtcatgta tatgtttttg tcagtggact ctcctttgtg gctatacatt
     4261 gtcgctactg ccatcatggg tgccggcaat gccttgttcc agtcccccaa taatacgatg
     4321 gttatgagct cggtcactat cgaaaacttg ggactggcag gctcgatgaa ttcctttgcc
     4381 cgaaatttgg ggatggtcag cgggatttcg ctttctacga cggtcttata tcaagcgatg
     4441 tctgctaaga tgggccagca agtaactaac tatatcgcaa atcggccgga tgtcttttta
     4501 tatggaatga agattacctt tttaggcagc tttgttttgt gttttgttgc ctttttcctg
     4561 actatctggc gtatcaaaaa acaaggaggt attcagaaaa atgtctgaaa aaaccactgt
     4621 tgaagaattt ttggaggcct gcgtatgggt gagtgggatt tccatggatg atggaagatg
     4681 aagtgaactt ctaaaagccg aaaaactcaa gtattatctt caaaatacta gtgctcaatg
     4741 agatgcttga tttgatcaca agaagactgc gaaagccgct ggcaaccgca agctaggtat
     4801 cccaccgatg gactacgttt tagaggctac tggttatgaa tacggagcca acgctctcat
     4861 ctgcataaga atacatcacc cagattatgt aaagattttc gatacttctg tcaaaaaatc
     4921 attaagatag ataaatgatt tccgtctagt caaattggtt cagaatgtag acacacagat
     4981 ggtaagaagt cactcacaat tcgagaatgg acttgtccta tttgccgtat acatcataat
     5041 cgaaacgtca acgatagtat caatattttg accaagggct atgaatacaa gcattgcctt
     5101 agaaaacatc aagaacctta ggaacaacgg ggatagcttg gttaataaga gataccgctg
     5161 tttaggcttg caagtatact cttttatcaa gaattccgtg gctttagtca tgagaggctt
     5221 aatactatga aaattttctt tagcattttt gatttaggtt ccttttctaa ttataataag
     5281 atgaccacta cagtaaccta ctgaaatata taaacagcca ctgtagtacc attttcaaag
     5341 tagtctatga gaggagaatc ttcattctta tgaacgctgg aattgcggat atgaatttaa
     5401 taaaaaaaac ccttaatgat tttacttcca attcaatatc aaaaggtact ggtatcaatt
     5461 taagcacaat aaaaaagctg aaaagtggtg aaagatcagt ggaaaagctg aatcttttag
     5521 atgctataaa aataacggaa tttgctatga aaaatggaaa agctgagatt gaaatatgga
     5581 gataattatc gtctagtgaa ttacttgcca cttagctgaa tttaacgatc cttggtgctt
     5641 gaagggggag attcttggga atagagcata cttgcaagcc tatgtcttta ctaacaacga
     5701 tatctcttat taaccaagct atccccgtag ttctaaggct tttactttta tctaagcaat
     5761 aatcgtattc aaaggaaatt tttgaccctt agaaagtctc attttatcag acgtgagctt
     5821 aggcattgct gaattttgtt aattcggttt taggttgtgc tttgagcata atatgaaagt
     5881 attctttatc acgatttcat tcatctaagg taatgtgatg tgattctgaa attctttcaa
     5941 aggcagtttt tgcataagct gacattacat catcaatgac atgtttacaa tgtttcacta
     6001 ctaaaataag ggggtaacca agaagaaaaa ctgaatgatt atttgtatct aattccatgg
     6061 tatatcaacc atttcctata tagactgatt atgacatgga ttaaaataaa aaggcactat
     6121 gtcttaattg tcggttttcg agttaccgaa tggcattcat cacccaccta tagaggatgg
     6181 gcgacttctg cctgaaataa gttacactac ttataacttc taataaaatt agattgagtc
     6241 attattattt aacatcaacg attttccagt ttccattttt ttgtaatact aatttgtact
     6301 gaaagtagct agtggttttt gatacattat caaggtattt tatctttgta ctcactagaa
     6361 aattttttcc gttttgtaga taaactggat tctctaactc cgaaaacttt aagtcaatat
     6421 tgattggtcg catcgcacca ttttcaacat aatactccaa ttctttttca gaagctgatg
     6481 gataaagttt aaaaaatgtt tctaaaaaat cggtgatttc ttccgttgtt ttggcatcaa
     6541 tttctgaatc gttttctact tgttttgctt catattttgc cttgtctggc tttccaatca
     6601 ttgttggcaa actagttact aaatgattcc tgttttcatc ttcaaagatt gtcactcgat
     6661 atgcagaaga aattgattgc gtcttttttc cctctgtaat atgttgctcc actaaaaatg
     6721 aaacgacaaa ttcttccgag tgtttttcaa catctagaat ttttacagat tgaacttcgg
     6781 aactcgtggg aatatctgct cgaaccatat cttggattaa agcgagaccg ttttctgtaa
     6841 ggtactgttc gagattactc attctctttt caattgcttc tttgttattt ttccatgaaa
     6901 aatattcttt cgcaaaatcc tctgtaaaac tttcaacgcc agaagtattc actaccttct
     6961 cttttataac cactttttca tgagttgtat gttgatcaat ggcggtgaaa tttttataaa
     7021 cgccaaaagc aagactacaa gacaataaaa gccaaagggc aagcaccatt tttcgatgct
     7081 tacccacgga tacaactcgt tcttttttct tttttgggct cttttccttt tgattccgtt
     7141 caatttttat cttcattctt tttcctcttt tctcttattt gtatcggcca gctccgataa
     7201 gatgctgccg ccaatagttt tccgttagat tcgtccaccc cactggatct cctgcatgaa
     7261 acatgcgatt ctcccctgcg taaatgccca catgggtcac ataagtaccg gcatcatagg
     7321 ttgaatgaaa gaaaactaaa tcacctggtt tggcatcttt tagatcaata tgagtcatgg
     7381 catcatattg atcctgagcc acccgaggaa gtttcaggtc aactttggca taacaccatt
     7441 gggtcaagcc actacaatca aaccccgttt ctggacttga gccaccaaac acataggccg
     7501 ttccttcata cttaaatgcc tcatcaaaaa tttcttggac tattttgtca tcaaactgag
     7561 ttgtagttag gtattgcttc actaataaac agtaaaacat attgccataa ttataccgcc
     7621 acccaccatt ttccttaatc gcgattgggt tggggtaatc gactttttga ccacctgatt
     7681 tttcctttga aaaagattca gctaaagcaa aactatgctt cttcccgttt ttcccgacat
     7741 gatctagata cccgccacca tagttatagg cctgaataat actatcttga tcacacccta
     7801 gttctttgga acgcttaacc aaagacgcaa aatagagaca gccttgtttg atggatgcct
     7861 cggtacttaa tgaattaggt ggtaatccgg ctgactcaga actttgcata acgtcttcca
     7921 ttgtgccacc agattccact tgaataatcg ccaaaagaat tggaatatac tctgttatat
     7981 ccgattcttt ggcatacttt tccaccattg cacggtgttt caatacttct tctgagacac
     8041 tagttccgcc aggagaagtg ccagaactac catttccctc actgtcttca ccaaacaaca
     8101 aacccacaca catcaaaata cccataaaag agatggtcag aaaagttaat gaaataacac
     8161 ccactttctt caagttcatt ttttctcacc tgaccttgat tttggacgat ggttcattct
     8221 tttttgtact ttttgattgg tctgctttgg cagagtggac ctctgtttca cttgttggaa
     8281 agaatttttc ttacttgctg attggctttt cccttttgaa atgatttctt gactagttag
     8341 ttttcgtttg atttcgggat tcagatttgc tctcacaatc cgctttggtg cttctttttt
     8401 cagcatcata tctttctgag gttgagctgc tttatctatt ggaatattcg cccttctttt
     8461 tccctgacga tttgttgctt cactcagtat cttgcgtttt tcgtccattt tcgcatttct
     8521 tcgttgtcgt tgtttttctc gcaaatccac tcgatccgct ttttcttgga acagtccgcg
     8581 cttgaattct tctggtgctt ttttcgactt ttcgattcct ttatggagat tgtatttcaa
     8641 attcgttggc gtattcctta cctgatcctt tacttgttta gtcttatctt tcaccaaagc
     8701 ttgggtatcc aaaactttgc cgatttttct cccagccaaa ttcattcggg attgtttctt
     8761 attatttgaa actggattct ccttagttaa gtcagatagt atttcgtgat cgttcggtaa
     8821 tcgtgaattc tttcgtagag aagaaccgga tggcttaaat ttggattttg attgttcctt
     8881 tgccaactta tttccgactg ctgcaccacc accaaaagcc aaagctcgtc ccacatttcg
     8941 attgagttgc cgcaattttc gattcattaa catttgcggt tttcgcatga cgcgtcttcc
     9001 cattgactgg ctatcattac tctggaggtt gaacatactc ataatgtcgc ccaatttgaa
     9061 atagataccg gcaaaggtca caatttgcag aaaagcaacc atgaaaaatg gataggttgc
     9121 cgaaatattg aaaaacatcg tggagatgct aaatgcagtc gtaatcacca aagtaacacc
     9181 cgctcgcatc ataatcgtat tgaataaacg aataatcgcc ttctttccta aactttcata
     9241 agtcggcaac atggacaata agaaactaat tggcagaaac atcgcaaaga taatgaagag
     9301 aatttgcgag aacaacataa tccctgttag aagaaaaaca aagaaggaaa tgccaatatt
     9361 gaacaatacc aaaaagacca cggtccctaa acggttcatg gtcttggtaa tactgagatt
     9421 cgcattgtca ttgtcttcaa tctctgcctt gaccgcttct tcccgatctt tgcctttgtt
     9481 ttcatctgga ctcacagata gaatcttgtt gacccggtct tcaccaattt cttctatgtt
     9541 ggaatcatca aattgcagta gtaaccaagg ttgttggact tgaatcgaaa acaaactatc
     9601 ccgaatcaaa tccacactat cttttccttg gctttctgaa ttgggtacaa cgatctcggt
     9661 tccaagggta agggccgctt cactaacatc tgagctaaag tcattgattt ttgtgatata
     9721 ggtaggcgca taagcaatga acgaacccga tagtagaaaa atcatcacaa aattaatcac
     9781 ggctcgaact gctttcgtgg tttctcgttt taataatccg gtatacgcca catacacccc
     9841 cagagctaaa atcatcaata atagaaaccc aaaataaaat ccggaggtct ggagtccatt
     9901 ttcagtaata ccagccaatg tttggatatt ctttccaata ctttcagccg tatccgaaat
     9961 gaaatctaat ttgtaagcct cttgaaccac ataacccgtg gcgttcgaca aatacaaact
    10021 caccgtccaa atgaaattag taatggcata aagaccatac atcacgcttt tccctaagcc
    10081 atcaccccaa ttccaaggca accaatccca agagctatcc acaaagaaat ctagttgata
    10141 gttgttcagt gaatatttgg aatatagatt tcctgcttca actgtatcat caactagccc
    10201 tgtcgcctcg gctaccgtac caactaaact caatagaact atcagagtga gtatgctaat
    10261 aaaaacgatt cccacaatcc gaaaaagttt ctttttcatc ccctcactcc attctctctt
    10321 gaacaggtgg acgggtatca aaggcatgga agagttcttc aaagactgga tgtacctgaa
    10381 ccacgcctac ccgaccatac aaatcctgca tcaagcattg gccattttcc aaatctcgta
    10441 ggcgtttttg attgctttca tcttctcgat ccaagccaaa gaattccaag gttttcttga
    10501 tttcatgaat atcggtactg cgaaacgcaa acttcaaccc gatattattc ttcaagcgtt
    10561 catccagtaa atcatcacta ttttgcgtga caaagtaaac cccggcattc atagcccgcc
    10621 cagcacgaac gagcttattc gataatgttt tcccttgcgc tacttgaaga aaactccacg
    10681 cctcatctaa gtctacaatt ttgaagattt ctcgattact atggataaag tccaaggcaa
    10741 aagtggaaat gacgattagc atggaaacac taagcaattc catggtggta tattcttcaa
    10801 aagtggtttc tgcatctggt aaaaccaaat ccgctacttg aatgatattt aactgcctat
    10861 ctaaactaat cgactgggta atcgaaccat ctgagaaaag caaatgggca aaatcataat
    10921 cggtaaagga ctcgatgtga tccgcgatat tttccgcaac tggtgagcct tcttttcgaa
    10981 gctcattaat tacttgtaat aatccccgtt gattgctttg ggtaaccgtc cgaatggcct
    11041 tacgcaacac tggaaacttc tccccatctc gactagaaat tcctgttaaa aaggtcagaa
    11101 tatcaattgc tagactttca gaatccttct ttttctccat aatcacataa ggatcaagta
    11161 atccttgatt ttttggctta ctagttaagt tgagaatatt gatttcatcc gcaatttctg
    11221 gcaaggtttc tttccagcca ccccgttccc cttttggatc gacaatcaca gcttgtccac
    11281 caaaaaggac ggcgtaataa actaagagat tgttactaaa ggactttccg ccaccgagtg
    11341 aaccgaggaa tgccgctgct agcgcattgg taactgaatc cttgacccct tgtgaggcca
    11401 atgccggttt caggtaaaca ttgcgccccg tatctacgtt ataaccaaag taaatgcctt
    11461 ccaactcccc taacatctga gtggcgccaa aaccaagtcc ggcgagaaaa tcagacgtaa
    11521 catactggat ataatcattc atgtatcgct tactagaagg taagaattct tcatgtagcc
    11581 ccatcatatc gccaaaaggc cggactagtt ttacattggt atcatcataa aaatctagca
    11641 cttcatcaca ccggcgcttt agttcatcta ctgattcggc acttactcga accacatagc
    11701 tcagtttgta catggattct ttggactgat ccaaggtagt ttccaattca tctacggaat
    11761 ccaatgcatc taagacattg gaatttgttt cattgtcaga ttgataggca tggttgtcca
    11821 agtcttttaa ttcctttttc ttattgcgaa cggtagcaag tgcctttttg ttcgtcacaa
    11881 tctccacatt catactagtg tcaattggga atgtaaattg ttgttgctgg taatagaaaa
    11941 gttctgagga gggaaactcc atttccccca caattgtatt aattgttaag tacgctacat
    12001 atgattcatg attctcatgt tccatgcata agtaccgggg cttttcttca atcaaacaac
    12061 gactgggacg taataagtcg taacgtttca ccaaagtttc cgacttcagc tttttctttg
    12121 gtagctgaaa ttcatattct tcaaatggtg tacccttttc gccatagata tgttcaatca
    12181 aatacatcag atcactcggt gtgactcgtc tgactttaaa tcttcgagcc aatttacttt
    12241 ccattagttt ttcaagcttg gtatatcggc gaatttcttc attggataag gaaacaaaat
    12301 cgcccatcaa atgatgattc actccataga caaattcttg aaagccggag aagaaagatt
    12361 tcttgagatt ctttagattg acctcttcat ctgtcgcaat cagtttgaag ccaataaaaa
    12421 agcgataatc aatctgacta tcgccaatca tggatactaa tgcctctgtc tgtaaatcaa
    12481 ttcgttgttt cgctacttct gttaatcgcc cggtaatttc cttttttgat cgttcttgcg
    12541 tggcacgaat actactttct gtggcaattt gtaaggcatg aatcttgcct tcacgatttt
    12601 gcgcaatcaa ttgacgaaag ttatcgtgga cttgatattt ctgttcaggt gacaaaaagg
    12661 agtaattgta aggtattaac tcatagtagg caaaacattc tccctcttga ttaaagacaa
    12721 gattattttc aatatactta atcgggtaca tagttcacac tccttaccag agtaatgggt
    12781 tcgtcccatc gtttcttctg ttctttgact ttctttcctg cgaaagtgac cttaaatcgt
    12841 aagtgatagc taataaaaga tcgtaaaaat cccaatggtt tcttaccgtc aaacgttttt
    12901 tgcgacatga accaagtaac tgccaccggt atacctaagt attttaaaaa ggccccctct
    12961 atcatagata gtggaggcac atgggcaaat acgatgacaa acagcaagga taaaacaaac
    13021 caagccatct ggttaaaagt cacaggaaaa ggcagttgaa agtcattaat tgcataaatg
    13081 accttttcaa ctgcccaaat actggtgtag ctttttattt tcttcatcaa atcattcctt
    13141 taaaaagaag gatagtttag aaatcaacta cccttcaata attttgttaa tatggatact
    13201 caaaaacacc tcgactcgtt accaaataat tgcctccaat ctccaagtct ctaccacacg
    13261 catcaaaatc aatatacgtc ttcaagtgtt ctggtacttc tccaaaatca ccagcttcta
    13321 caatcaagaa atatgctaca tcactcatat catcgcaatc tggataatag ataatatcat
    13381 ccacatgctc taccatctcc tcaaaagaat tgaagaaagc atgttgaatc tcagaagcaa
    13441 cttcaccaac tggcgttcct tcaagttcct ccgctaagcc acacaaccga ttaatttcct
    13501 caattggcgt atactcatca atctcaaagg gaagttcata atcatgaatt gcatattcct
    13561 cgtattcgtc atttaaccca attcgttctt tcacttcatc aaaatcgaca ggtggggtaa
    13621 accacgcccc caccaattca ccttcgttat atttcccgag gttcgcaata taaacacgca
    13681 tctgttccat cgtgcttccc ccttacgcac cgacaattcg gttaaacaaa ctgagtaaga
    13741 catccttcac cccagaagca ttaaagacca atccgaccgc aatcaaagca atcaccaaaa
    13801 agccaatcag tttggaaaac tcccgcttaa atcctaaaaa caaaccaatg accacaattg
    13861 ccagtaagac taaggattgg gcattggtta aaaaccatt
//
LOCUS       DAPBCS010000068        13084 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPBCS010000068 DAPBCS010000000
VERSION     DAPBCS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.071"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(78..2198)
                     /locus_tag="RD521_002490"
     CDS             complement(78..2198)
                     /locus_tag="RD521_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDT7772165.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="RD521_002491"
     CDS             2428..3606
                     /locus_tag="RD521_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDT7772166.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="RD521_002492"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="RD521_002492"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDT7772167.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="RD521_002493"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="RD521_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDT7772168.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="RD521_002494"
     CDS             complement(4959..6212)
                     /locus_tag="RD521_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7772169.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="RD521_002495"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="RD521_002495"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDT7772170.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="RD521_002496"
     CDS             7789..9135
                     /locus_tag="RD521_002496"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDT7772171.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="RD521_002497"
     CDS             complement(9443..9829)
                     /locus_tag="RD521_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7772172.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="RD521_002498"
     CDS             complement(10097..10246)
                     /locus_tag="RD521_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772173.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="RD521_002499"
     CDS             10708..10920
                     /locus_tag="RD521_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772174.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="RD521_002500"
     CDS             complement(11198..12997)
                     /locus_tag="RD521_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDT7772175.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaatca aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaa
//
LOCUS       DAPBCS010000069        12238 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPBCS010000069 DAPBCS010000000
VERSION     DAPBCS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.072"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..610
                     /locus_tag="RD521_002501"
     CDS             <1..610
                     /locus_tag="RD521_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7772176.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="RD521_002502"
     CDS             723..1001
                     /locus_tag="RD521_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDT7772177.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="RD521_002503"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="RD521_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDT7772178.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="RD521_002504"
     CDS             1458..2213
                     /locus_tag="RD521_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDT7772179.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="RD521_002505"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="RD521_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDT7772180.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="RD521_002506"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="RD521_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDT7772181.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="RD521_002507"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="RD521_002507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDT7772182.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="RD521_002508"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="RD521_002508"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDT7772183.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="RD521_002509"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="RD521_002509"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDT7772184.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="RD521_002510"
     CDS             6950..7492
                     /locus_tag="RD521_002510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDT7772185.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /locus_tag="RD521_002511"
     CDS             7515..9071
                     /locus_tag="RD521_002511"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDT7772186.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="RD521_002512"
     CDS             9085..9987
                     /locus_tag="RD521_002512"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDT7772187.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="RD521_002513"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="RD521_002513"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDT7772188.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="RD521_002514"
     CDS             11430..11852
                     /locus_tag="RD521_002514"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDT7772189.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4071 a         2166 c         2698 g         3303 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tcttagaata
    12181 atttggacag aacagaaaga agaaaacctc tttctgttct gtttatttgg ttaaaaat
//
LOCUS       DAPBCS010000070        11802 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPBCS010000070 DAPBCS010000000
VERSION     DAPBCS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.073"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(113..874)
                     /locus_tag="RD521_002515"
     CDS             complement(113..874)
                     /locus_tag="RD521_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDT7772190.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1194..1439
                     /locus_tag="RD521_002516"
     CDS             1194..1439
                     /locus_tag="RD521_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772191.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(1623..2372)
                     /locus_tag="RD521_002517"
     CDS             complement(1623..2372)
                     /locus_tag="RD521_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDT7772192.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            2558..3262
                     /locus_tag="RD521_002518"
     CDS             2558..3262
                     /locus_tag="RD521_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDT7772193.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            3302..3859
                     /locus_tag="RD521_002519"
     CDS             3302..3859
                     /locus_tag="RD521_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7772194.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            3867..5006
                     /locus_tag="RD521_002520"
     CDS             3867..5006
                     /locus_tag="RD521_002520"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDT7772195.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            5018..5449
                     /locus_tag="RD521_002521"
     CDS             5018..5449
                     /locus_tag="RD521_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDT7772196.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            5463..6908
                     /locus_tag="RD521_002522"
     CDS             5463..6908
                     /locus_tag="RD521_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDT7772197.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            7194..8819
                     /locus_tag="RD521_002523"
     CDS             7194..8819
                     /locus_tag="RD521_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDT7772198.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            9040..9180
                     /locus_tag="RD521_002524"
     CDS             9040..9180
                     /locus_tag="RD521_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015371017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772199.1"
                     /translation="MYVLIEVLWLQAAISLDKIKDRRNQVKKAVSKETNDQVCFTLKK
                     MQ"
     gene            9451..9804
                     /locus_tag="RD521_002525"
     CDS             9451..9804
                     /locus_tag="RD521_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772200.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
     gene            10585..>11802
                     /locus_tag="RD521_002526"
     CDS             10585..>11802
                     /locus_tag="RD521_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772201.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKIL"
BASE COUNT         4148 a         1665 c         2190 g         3799 t
ORIGIN      
        1 actcgatata actaatttga ataatatata taataaaagg ctattgttct taaaatataa
       61 atagtatata aagagattga aggtaatgct cgaatgaaca ttaccttcaa tcttaatgat
      121 gcttataact aacgtaataa aaacctgaaa caaaaatagc tccgccgatg atatttccaa
      181 tataaacggg gataaaattc gagagaaatt ctaaccaagt cgcttgacct tcaaatatgg
      241 cagcaggtat cacaaaacag ttggccacac tatgctggaa accgattgcc acaaaaatca
      301 tgactggaaa ccaaatcgtt agtactttgc cggccgcatc ttttgctcca tatgataacc
      361 ataacgctaa accgacaaac caattacaac ctattccgga gcctaatgct tgccaccaag
      421 aaacagcgat tttatgatca gcaatatgaa tcacttggtc gagatagaca cctgaatgag
      481 ttaaaccaac aaagtgtcca aagaaatagg caacgaaaat tgcaccaata atatttgcaa
      541 aagtaatcac tgaccaattc ttaaccagct gtgagaatgt gactttttta tccataaagg
      601 caatcgccac agccatcata tttccggtga tcaattcacc accgccaaga aggatgacca
      661 gcaggccgat aggaaaaacc gaggcaccaa ctaaactagt caagctagcc atagattctg
      721 ggatagaagc agagactcgt acgtatgcca aataacctag agaaatcatg gctcctccga
      781 taaaacctaa aatcagttta gctaagatag gtttatgtat tttttttgtt ccaagatgaa
      841 tactgagttc cataatttct tctggtgtgt gcataatact tcgattccta ctttcttaat
      901 gaattttcat gtgataaaat atagtgaaaa aaatcacatt ttctttgttg attatatcat
      961 cttattattt atatgtgcat attattgtaa aattttctat ttaatcacat ttgattctaa
     1021 aaaagaaaca aaaataaaag tcatgtatat ggtggtcaaa aagtaaataa tatcagttat
     1081 caaatttcct gtaaattttc ttgaaactac agcatattat tacttcaaaa caatctacat
     1141 tttttataaa aaaggaaatg aataactgaa aaaattatta taggagagaa aaaatgtcaa
     1201 aatctaaaaa aaatatcacc ataattggtt taggcttaac cgtatattta tttacagaat
     1261 ttgtttatag acatgtgaat ttatttgcag aacctttatt tgtattatac gaatcaaatt
     1321 cctcaatcgt cagtgaactt gctaatttaa tgatagcatt gctgtttact cccgcgttga
     1381 gagtcataac tattttggtc tgtttagtgc tattgttttt tgttaataaa aagaaataac
     1441 gtagtatttc aaaagacaaa gaagtgggat gatgattgct atagtatgtt atacgtggtg
     1501 ctaggtaagt gagaataaac aaaaaaggaa acacgcaata aagtttattt caaaacttta
     1561 ttgcgtgttt cttttttaga ttttttatat ctgttgcttt agtagttacc aatagttttc
     1621 gcttaatcaa tcaaaacgat tttttgttta ttgtattctt tacgaacctt ttcaggaatt
     1681 tcttctgaaa taagataggt aaggtcttca attggaccga cgatatgatt actgatggtg
     1741 ccgaatttat cattagttat catcccaagt acttcagtag aaatacgtat catctcacgt
     1801 ttaatgtcgg attcttgcat agttggcacg ctcgttccaa catctagatc tatgttataa
     1861 atacccataa tataaaggtc ggctcgaatg ttttgtagtt gcttataagc ttgtacccca
     1921 atattgatca tagattgttt ataaaaagta ccgccaatat tgataatttc caaattagga
     1981 tgctgtgcta atgctatggc aatcggcggg ctattggtaa tgattgttgc acgaaaatct
     2041 tgtgggattt cttttgctaa agccaagtta gttgtacttc catcaatgat gatgacagat
     2101 tcttctttta aaaaagggag ggctttatga gctaatgcag ttttttgttc gctagctaca
     2161 gtttctcggt agttaaaatt ggtaattggc ggaccaacac gtaaagcgcc gctgtgtact
     2221 ctttttaaga gaccttggct atccaaatct ttcaaatctc gtcgaatcgt gtcttcggat
     2281 acattgaaac gttgacttaa atcactagcg ataacttttt gttctgcgtt taataaatct
     2341 agaatttttt gatgacgttc taatttcaac atgttgcgtg ctccttctta tttattcttg
     2401 tttatcatgt catttatgcg tttttatgtc aataatttcc gtttttaata cactaaaaag
     2461 gcataaacaa gcaaaaacaa aatattttta tgcagtttta tgttgactag tatttgttaa
     2521 gcggttacaa tgagggggta attaaaggag ggaatgtatg gagaaaaaaa ttattttgtc
     2581 gccttcactt atgtgtgggg atctagcaaa tttggaagga agcatccaag aactagaaag
     2641 tattgggatg gattccttgc atattgacgt gattgacggt tcttttagtc ccagtatgcc
     2701 tttagggatc gaaacaatca aaagaattag agaaatcact tcaatgaatt ttgatgtaca
     2761 catcatggct aaggataacg ataacgaatt ttttattgga gaaatgttga aaattggcgt
     2821 agagagcatt acttttcatt atgaaacaag cctccatatt gatcgatata tccagctcat
     2881 caagaattct ggaacaaaag tagggatagc tttgaatcca gcaactccgt taaatgtatt
     2941 acaatatatt ttacccgaga ttgataggat ttgtttgatg ctgattaatc caggttttgc
     3001 aacaaacaag tcagaaaaac aagtttctta cgcgctaaaa aaaattacag atctttgcca
     3061 gttagtaaat gagaaagagt tagcagtaga tatccaagtg gatggacggg tatcgttaga
     3121 aacgatcccg ggattattag atgcaggcgc aacaaatctg gtattaggaa gtacaagttt
     3181 gtttcttaaa ggaaatacat tagcagaaaa tcaaaaacta ctttatcaag cgatgaagca
     3241 atcagttagg aaaaatcaat agatgtagtg gtcctgaata atagaaatgg aggggaaata
     3301 gttgagttat aaggcggcga aacaaaatat tgtaagagaa attactgaaa cattagaccg
     3361 tatttcttca aaagatgtgg aacaattgat aaagaaaatt gaaaaagcgg aacgagtttt
     3421 tttcgtgggg gttggcagag tactgttagc gctagaagga atggcaaaac gcttagggca
     3481 tctaggaatt cagacagtag tcgtaggaca aatcactgaa ccggcaatta cagataaaga
     3541 tttattgatt gttggttctg gtagtgggga aagtgctttc ccactaacta ttgcaaaaaa
     3601 agcaaaggaa tttgggtccc aagttgccca tataggatca aacccagaca gcacaatgaa
     3661 agaatattca gatcttttta ttcgtatacc cgtacaaaca aaattagcgt taccagagga
     3721 aatttcttct atacagccaa tgactagcct gtttgaacaa agtgttcttt tgttaggaga
     3781 tagtattgct ttagtgatga tagaagaaaa agcaattgat atgtcaaagt tgtggcaata
     3841 ccatgctaac ttagagtagg tgaaaaatga aagcggtaca ttttggagca gggaacatcg
     3901 gcagagggct tattggacaa attctacatc agaatggatt cgatctatgt tttattgata
     3961 cgaatcctga actgatccat caattaaatc aagataaggg ttatactatc gaaattattg
     4021 atgaaatgaa caggagtgat ttcattgatc atgttgtggc actgaatagt ttatccgagc
     4081 tagaagaggt gattacagca acaaaaaatg cagatatctt aacaacatct gttggcgcaa
     4141 taaatttagt aaaaattgca ccgatcattg cgcaagcctt aagtaagcga tttgaacaaa
     4201 agcatagcat caatatcgta gctaatgaaa atgtgataaa tgcaagtgat gtgttgaaaa
     4261 aagaaattta ttctttgctg tcagaaaaag aacaagagaa ttttgatgaa tatgcatatt
     4321 ttgtgaatac ggcaatcgat cgacaatctt taggaaaagt tcttggagga aaaatgatct
     4381 ctgttgtcga accttattat gaatggataa tggatcgaca gcagttagat ccaactacat
     4441 cttttgatat gaaaaatgta aatttggttg atgatatgga gccttacatt gagcgaaagc
     4501 tttatatcgt taatgctgct catgcggcag tagcttattt aggcgcactg aaaggatata
     4561 agacgattca agaagcaatc caaaatatgg agattttcaa tctagtcaag caatttttaa
     4621 tggagaatat agagtacttt gtacaaaaat atcatttttc tagagaagaa ttaacatctt
     4681 ttgtggaaaa aacattaaac cgtcatggga atcagaaact ctccgatgaa attactcgtg
     4741 taggaagttc ccctattcga aaattaggcc ctaatgatcg tttaattgct cccatagtaa
     4801 aactagattc atatcattta ccttacgatg caggaataaa agtaattgct gcgggatatc
     4861 attatcggaa tcttgctgat ccagaagctg aacaactacg tcgaatgatt gtaaatgatg
     4921 ggttgaaagc aacgattaaa aagatttctc gtttggaagg taagctgctg aatgaagttg
     4981 tcgctgctta tgagtcccag atttagagga ggagaatatg ctaaaagaac aattgtcagg
     5041 aaatacaatg tttatggatg cgatcgattc atggcaagag gcaatccgag taagtgcgcg
     5101 tcctttgata ggaaagggaa taatagagga aagctatgta caagcgatga tacaaaatgt
     5161 gttggataac ggaaattata ttatattact gccactagta gcaatgcctc atgcgcgtcc
     5221 agaatttggg agcaaagggg tcgggatgag ctttttatgt ttagataagc cagtcatgtt
     5281 cccaagtgaa gaaccagtaa aattcttctt taccttttct tctgattcgc caaagggaca
     5341 tttagactta atggccaatc ttggagaatt gctgagtgat gaaaaaatgt atcaaaaatt
     5401 attcgatgtg aagacagaaa aagcattgtt agatttgata aagaagtaaa ggagagaaga
     5461 aaatggaggt agcaaacaaa aaaaaacgag tctccatgcg agctaaagtt caagcgattg
     5521 gcggtttctt aacaaatatg gtattgccaa atataggcgc atttattgca tggggaattg
     5581 ttactgcatt attcattcca actgggtggt tcccaaatga atctctgaat gaactcgtaa
     5641 gtccgacagt cagctatcta ttgccgttac tacttgccta tactggtggt cggatggttg
     5701 gtggtacacg tggagctgtt ttaggtacaa tcggaacaat aggtttgatt attggtgctg
     5761 acataccaat gttcatggga gcaatgatta tggggccact tggtgggtgg atgatgaaaa
     5821 aaacagatga ttttttagat gggaaagttc ctgctggttt tgaaatggtc gtcaataatt
     5881 tttctatagg aattattggt ttcatactga tgtgcctttc ttatctggta gttggaccaa
     5941 taatccaatc agctaatcaa tttgtaacga gtgcaattga agcattagtt tcaactggtt
     6001 ttttaccgat actgtcctta atcaacgaac cggcaaaagt cttattttta aataatgtta
     6061 ttgaccaagg tgtttattat ccattaggta tgcaacaaac attagaggca ggcagatcaa
     6121 tttatttcat ggtagcatca aatcctggcc ctggcttagg actattgtta gcttattctt
     6181 tatttggtaa aaaggcagca aaacagacag caccaggtgc aatcatcatc cattttttcg
     6241 gcggaataca tgagatttat tttccatatg tattaatgaa accactcact atattatcta
     6301 tgattgctgg tggaatgaca ggaatcatca cttttgaatt atttgatgcg ggcctggtag
     6361 caggtccaag tccaggttct attttttctt atttagctct aacgcctcgg ggaaattttt
     6421 tgggtgtcat ggcgggagtg gtcacagcaa cactggtttc cttttttgta acatcagtaa
     6481 ttttaaaagc agataaatca acagatgaag acagaaattt tgaactatca gttcaaaaat
     6541 cgaaaacgat gaaatcagaa ggaagaaaat tattaaaaga agaaaatgct agagaaatat
     6601 gtaccgagaa aaaagagatg aataaaattt cctttgcttg tgatgcagga atgggaagca
     6661 gcgcaatggg cgcaacaata ttcaagaaaa aattgcaaaa agcaggtatt actgatgttg
     6721 agataaaaaa ttatcgaatt gaagaggtac cagaagatag tgatttggtg atcgtacatc
     6781 aagatttgga ggaacggact agaaaagcac atcctaacat gaaaattgtg acattaaaga
     6841 attatttgca agatccagta atcaatggat tagcagaaga aatcattgga ttaaaaacag
     6901 aagactaatt tatgggaatt tttgattaac ctttattaaa attattttgc ctaatataaa
     6961 aaggttgtga catggcttgt gtcgcaacct ttttttgtcg cacaaataat taatcggata
     7021 ttttatgttc atattttttg tgatcaacga gaactgatgg ggcaaagata cattttagat
     7081 acttttaact gttaatttac aaaacaaggt gaaacgaata gaaaaatgaa aaaatcgata
     7141 cggaagaata tgtttgatta aaacatgcat gaagagaagg aggttgatga tacatgaaaa
     7201 aaatatattc aaaggaaatc gattgtttta gaggcattgc tgttttagca gtgatatttt
     7261 atcatttgcc aaatagtttg ctgccaggcg gattgcaagg agtaacgatc ttttttgtgg
     7321 tttctggttt tttaatggct aaaaatacga taagagatga aaaaaacaga caattcagtt
     7381 ttttacattt ttactataag cgtatcaaac gtatttatcc atttttactg ttttctttgg
     7441 tcgtattcat tttcatacta gggattagca acattcgatt gataggaaac attcgtagtg
     7501 agctacccag tttgattttt ggctataaca attggtggca gctgaatcaa ggagtatctt
     7561 attttgactc ttactttaat gcttctttat ttaaacatta ttggtcgctt tcagttgaac
     7621 tacaattcta tttgatatgg ccatttttgt ttattgtcat taagcgtatg agaagaaaac
     7681 aagtgtttta cttaatctat acattgattt ttgtttctat tctttttagt ttgtttttac
     7741 cttcaactaa ggcatattat catacgatag ctaaactttt tccattttta ttaggagttt
     7801 gggggtattt taatcgaata actattggcc gtttctttga acaaaacact tttagtaaaa
     7861 tctggctttt attactagct agtctttgtc tgattctgtt cccgattttt ccttatacac
     7921 taaatgaact actgatcagt atttgttttg ctctattgtt agccagtgtc gatgatatga
     7981 acattgcaaa aaagaccaaa atcgctagta caaaactttc ttttgtagga aaaatcagtt
     8041 atgaacttta tttggtgcat ttcccagtat tgattgctct gacaaatcta ttcaaacatc
     8101 accattggaa atatttggat atcgcaggtc tattcactgt gtttttatgt acattactca
     8161 tgattacttg ctttagaaaa ggagtgcaaa agatgagatc acccaaaatt tggacagcta
     8221 tggcactaat gataatcgga gtttctgtac tggtattacg tgctcctgcc agtcgactga
     8281 ccgctgacca aaatcagttg caaagtttat taaaagaaaa ctctagtaaa gcaaatgcag
     8341 gagaaaatct gttgtcacaa gagaagaaaa tattatttat cggtgattct gttatgctgg
     8401 gggcgtatca ggaactaaag gagacattta aaaaagaagc attggtagat gcaaaagaat
     8461 caagacaaat cactgcgtta ccagaaatct taaaggagca taaagattta gtttcttacc
     8521 aacaaatagt gatcgggtta ggaaccaatg gtgtgataac agatgaagct atagaacaaa
     8581 caatgtcttt gctgaaagat aaaaaagtat actgggtaaa tataaaagca ccaactggct
     8641 ggcaagatac gataaatgcc acattagcta cattgccaaa tcagtattct aatataacga
     8701 tgatcgattg gtatagtgag agcaaaaatc atcctgaatt tttttatgat gatgaaaccc
     8761 atttgaatga aagtggacgt acagcttatg caaagtatat tgcttcagtt attcaataag
     8821 gtagtgatta cgtgtgttaa ccttactttt tcctaatagg tgaatcaatt taaataaaaa
     8881 atacttcata tgataaacgg tgtttaagag gaggagcttt ttaatattcg tggtaaaaaa
     8941 gagatgggcg aggtcgttaa ctgagagctt cttggaaaga aatcgctttg gatttatcct
     9001 ctttgttata cagaacatga gcgagacgac aatactagga tgtatgttct gatcgaagtt
     9061 ttatggttac aagcagcaat cagcttagat aaaattaagg atcgtaggaa ccaagtaaag
     9121 aaggctgtta gtaaagaaac aaacgatcaa gtatgtttta ctcttaagaa gatgcagtga
     9181 ataaataata gtaatataat gctagaaggg actgtgacaa aagacgtgtc acagcctttt
     9241 ttgttatttt aattatcgtc cagtcttttt ttataaatga ttgcttaagc attaaatatt
     9301 aaaaaatata gagttcaaaa ttactgcaca attagatgaa aaatttgaac aacaaaaagt
     9361 gtatttttat actataatag cttttgaatt tagttactct attcaaaagt tatgctatgg
     9421 acaagaaaaa gaataaatag aggtgagaaa atgaatagtt tcaagaaaaa atttgtgagt
     9481 agatttttaa atatgaaaaa tttttttgaa aaaaagggag attatgatgc atataattat
     9541 gtattaaatc tagaaaagca aacatattat acactacgta aaaacgatga taatgacttt
     9601 tggactatgt tacctaaaat gctttcgatt gactctaagc tttccctttt agaggaattt
     9661 attaatttag acattgaaga attttgcggg ggaaaagagt taattaatat ggtagagagt
     9721 gattatcata cctattataa agaaagttgt ggatacaaag tggatgagca atgtccacaa
     9781 tcaattattt tttacattga gtaatatgaa ctattaagca ggaaagcaac tagaagctac
     9841 tgatttagta tttacaaata taaactatat tgctaacaat tattttctag aaggaataaa
     9901 agaactaaga atcaagacgt tatcttgatt cttagttctt ttaacatgga aaagatggat
     9961 aatgcactgt taattcatga tgcatcatca gaaaagttgg aaggagaaga tagaaatcat
    10021 taaatgattt ctacagatgg taaatgaaaa aacaaaatat tagcaaaaat ttgcctaaat
    10081 agatagtaac actttctaaa ttaaatacaa gcgcattcat aaaatgttta tacaaatatc
    10141 tatgtgataa aaattggata tgttcacaat aaataggaat taaaataacg aatttacgtt
    10201 tgtttttatt taccaatcaa attatcttaa tgcaggaata aaaaattaaa ttacatattg
    10261 ttaatcattt acatctccat taataactca aaatgaaaag atgtttttat ttttattcta
    10321 tatttcagaa aaaaattaaa ctagattata ataattcttc atgtaaaaat aaaaacaatt
    10381 acagctatga tgtttaagat aaaacaatta tgaaatcgat atccgtttat atacttatca
    10441 taagctagaa gcaaataact agcctatgta tgtaagtgag attatattta ttaaaaggct
    10501 tcttttgttc gatcagttat agtcgaacga gagaagttta ttctttgatt aaaataaaca
    10561 aaagtaactt attgaggtga aatgatgcaa acttttttaa ctaaaacatc tttcaaaaaa
    10621 gtagttttat tagattattt gttagaaaaa gacaattggt gtgcaatgga agaacttaga
    10681 aatttactta atgttacgga gaagtcagtc ttacactata tagaagaact agaggattta
    10741 tttaagcaat ataatggtaa tatccttctg aaaaatgaag ataataaacg atttttcata
    10801 aaaaaagaga aagattttcc tatttataat atttatcttc atttttataa agcttcttat
    10861 aattatcatt tgattgattt tatgtataaa tatcctagaa gtgttctaaa agattttgct
    10921 aaagagcaat ttactagtgt atctactgtt ttcagatatg cgaagctctt aattccatat
    10981 tttaggcgtt atcatataac atttcatcca tttcagttag aattgaatgc atcagaggca
    11041 aacattcgta gcttttttta ttatttctat tggaattcta cacgtgaaag tagtgataag
    11101 tggccatttc atatcgaaca aaaggaaata gagaagtata tagttgcttt tgaggggatt
    11161 tatgatatta cgttaactat ttttcaaaaa cgtgtatttt ctttttggtt agccattaat
    11221 atagaaagaa gttcatttag aaaagtacga gtagataatg aatataaatc agttatctca
    11281 gatgatccgc attttaatct tttgaagaag tggtcaaaac aaatcaatct ttcttttaac
    11341 agcgatgaac tgtgtttcct ttatcgaata atttattctt ttggagtaat tgatggaaat
    11401 gcaatatatg aaaatagtca tgcatatgca catcagcgac aaaatacttg ttcttatcga
    11461 gcagttgaga atttggagaa agttttacaa agcatgttcc gtttttcttt agatataaag
    11521 gatccagaac tcatattcaa ttttattgca tttcatgaaa gaagttatct attttatggt
    11581 aatccagatc ttttttttaa tcgttcgtat atcgaagaaa tgaaagaaga agaacctcga
    11641 acttatcaca taatggaaaa actcaaaaaa gaattacaag caaatgcgga tttggatgta
    11701 tcaaagaaat tagaaaactg ggcacaatta tttttggatt attattatgt gttagattat
    11761 tatgatttat ttttaacaaa tgttaaaccg attaagattt ta
//
LOCUS       DAPBCS010000071        11445 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPBCS010000071 DAPBCS010000000
VERSION     DAPBCS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.074"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            185..1183
                     /locus_tag="RD521_002527"
     CDS             185..1183
                     /locus_tag="RD521_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDT7772202.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="RD521_002528"
     CDS             1295..1804
                     /locus_tag="RD521_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDT7772203.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="RD521_002529"
     CDS             complement(1834..2493)
                     /locus_tag="RD521_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDT7772204.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="RD521_002530"
     CDS             complement(2495..3259)
                     /locus_tag="RD521_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDT7772205.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="RD521_002531"
     CDS             complement(3267..3944)
                     /locus_tag="RD521_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDT7772206.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="RD521_002532"
     CDS             complement(3981..5366)
                     /locus_tag="RD521_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDT7772207.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5335..5589)
                     /locus_tag="RD521_002533"
     CDS             complement(5335..5589)
                     /locus_tag="RD521_002533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772208.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="RD521_002534"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RD521_002534"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="RD521_002535"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="RD521_002535"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDT7772209.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="RD521_002536"
     CDS             6305..7162
                     /locus_tag="RD521_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772210.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="RD521_002537"
     CDS             complement(7243..7578)
                     /locus_tag="RD521_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772211.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="RD521_002538"
     CDS             complement(7645..9015)
                     /locus_tag="RD521_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDT7772212.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="RD521_002539"
     CDS             complement(9093..9629)
                     /locus_tag="RD521_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDT7772213.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="RD521_002540"
     CDS             9938..11419
                     /locus_tag="RD521_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772214.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3517 a         2300 c         1855 g         3773 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatggtactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttatt
//
LOCUS       DAPBCS010000072        11148 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPBCS010000072 DAPBCS010000000
VERSION     DAPBCS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.075"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="RD521_002541"
     CDS             6..305
                     /locus_tag="RD521_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7772215.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="RD521_002542"
     CDS             378..1739
                     /locus_tag="RD521_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDT7772216.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="RD521_002543"
     CDS             1761..2021
                     /locus_tag="RD521_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772217.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="RD521_002544"
     CDS             2035..2787
                     /locus_tag="RD521_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDT7772218.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="RD521_002545"
     CDS             complement(2881..3486)
                     /locus_tag="RD521_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDT7772219.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="RD521_002546"
     CDS             complement(3542..4516)
                     /locus_tag="RD521_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDT7772220.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="RD521_002547"
     CDS             complement(4894..5613)
                     /locus_tag="RD521_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7772221.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="RD521_002548"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="RD521_002548"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDT7772222.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="RD521_002549"
     CDS             7164..8561
                     /locus_tag="RD521_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDT7772223.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="RD521_002550"
     CDS             8642..9082
                     /locus_tag="RD521_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7772224.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="RD521_002551"
     CDS             9229..9555
                     /locus_tag="RD521_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7772225.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="RD521_002552"
     CDS             9556..10305
                     /locus_tag="RD521_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772226.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="RD521_002553"
     CDS             complement(10331..10741)
                     /locus_tag="RD521_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDT7772227.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3536 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaatt
//
LOCUS       DAPBCS010000073        10937 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPBCS010000073 DAPBCS010000000
VERSION     DAPBCS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.076"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..154
                     /locus_tag="RD521_002554"
     CDS             <1..154
                     /locus_tag="RD521_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7772228.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
     gene            complement(235..1218)
                     /gene="istA"
                     /locus_tag="RD521_002555"
                     /pseudo
     CDS             complement(235..1218)
                     /gene="istA"
                     /locus_tag="RD521_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1613..2455)
                     /locus_tag="RD521_002556"
     CDS             complement(1613..2455)
                     /locus_tag="RD521_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDT7772229.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2780..3196
                     /locus_tag="RD521_002557"
     CDS             2780..3196
                     /locus_tag="RD521_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7772230.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3193..3663
                     /locus_tag="RD521_002558"
     CDS             3193..3663
                     /locus_tag="RD521_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7772231.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3677..4450
                     /locus_tag="RD521_002559"
     CDS             3677..4450
                     /locus_tag="RD521_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7772232.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4464..5276
                     /locus_tag="RD521_002560"
     CDS             4464..5276
                     /locus_tag="RD521_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7772233.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5330..6295
                     /locus_tag="RD521_002561"
     CDS             5330..6295
                     /locus_tag="RD521_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7772234.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6432..9155
                     /locus_tag="RD521_002562"
     CDS             6432..9155
                     /locus_tag="RD521_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDT7772235.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            9168..9800
                     /locus_tag="RD521_002563"
     CDS             9168..9800
                     /locus_tag="RD521_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDT7772236.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            10005..10232
                     /locus_tag="RD521_002564"
     CDS             10005..10232
                     /locus_tag="RD521_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDT7772237.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10301..10567
                     /locus_tag="RD521_002565"
     CDS             10301..10567
                     /locus_tag="RD521_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7772238.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10557..10826
                     /locus_tag="RD521_002566"
     CDS             10557..10826
                     /locus_tag="RD521_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7772239.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3626 a         1742 c         2137 g         3432 t
ORIGIN      
        1 ttccttagaa aaattaggtt gtcaaaattt caactcacgt tctgttaaag ggctagagag
       61 tagatttgaa agcatattac tggcttacag tgttctatta agtcgagcac aacgacgttt
      121 tgaaggaact ttgagatatt ctttaggata ttaaaattaa ctagcaccac gggtaaaata
      181 aatagttttt ctatcaagta tattgaacta cgataaattt gttattttgt tttgtttaat
      241 ttcgttgtac ttattggctt tgacttcgat ttctttaaat actgggtaat cggtgtccgg
      301 caaagctagc aaatctgttt tttctgtgtt ccatagatca ttgataatga catgtttttc
      361 gtaatggatt cgctctcgat cgtgtgacag ctccaccgct aatgcctcgt tgaaactggc
      421 gaaatccttc ataatcggtg tagatgaaaa gaagttgtac cgtacatagc cgactttgtt
      481 ttccatacta cctttctcgt ttccgctacg tggattgcac acctgtgtct caaaaccata
      541 gtgcatagaa aatcggagga aaccgtcagt cagcaccgct tgttcagtct ttgtttttgt
      601 ctaagtaacg gccgttgaca tattatcaat ccgtattttc cgaggaacgc cacctgattg
      661 gctgaagagt tctttcatgc cttccaacaa acattcttga ttctcggcgg ggagtacaac
      721 ggcgaatcct gcattactaa aagggaaagt catcactaat accttcacat ctttaaacgc
      781 tccatcgtga caaacttcca tcgtaccaaa atcaagttgc gcttctgctg gcgggtgttc
      841 aagacgctca aagccttggt cttccagtgt ttccaagtag tgtgtcgctt tccaatcttg
      901 aatgaataca cagactgttc ggtaggatcc tggaaagcca tcggctttta aagcagtagt
      961 cattgcttca gcagtcctac gctttttatt tggcagctta gcatcttctg aaagccatag
     1021 ggaaacgatg cctccccact tttctgtata catcatacca gtctttttgc gtgctttagg
     1081 ttgcggcagc aaatcatcat ctgcatattt tttagccgtg cgccaattta catctagctc
     1141 ctttgcaata tgattaatgg atttatcatg attatttcgt aaatttctga tagtattaat
     1201 atcggtcatt gtgagcatac ctttcttacc tccagttaca gtgtcgagac tataacttta
     1261 gaggagtttc aggttgcccg caatggcttt ttatcttaac ttaatcgatt tgcggagttg
     1321 tgtagttttt cgttgcactt gtatgtactt ttattctgcc ataaacagct taatgaatgt
     1381 ccacttgaaa aagtgaataa acgaaataga tgactcgatt taataaggaa gtaaacccac
     1441 acagaaatat aggtgatttt ccttgaatga ccaagatatt gaaagttagt gaaaaaacac
     1501 aaaaaggtag ccctattcac agaaggtctg ccttttgtgt tatcgccagc aaacattaga
     1561 gggggtattt catatagctt ttgtcctctc gacagtccta tcagtttctc ttttaactta
     1621 tctctaagtg ctgtccagtt ttaatagctt ctagcttgtt tcctaactgt gtttttaata
     1681 tgttgaaggc ttttgaacct gtgcagtggc aagtaaggat tttttctact cgtgagtcat
     1741 tcaatttatt tccaattttc attacctctt gctcaggtag acctaaatta tttatataag
     1801 gtatgccaat catatggaag ccaccaatta ggtattttat gggtttagtc ggaaatgttt
     1861 caagagcaat ttccatcata tcgataatgc catgatgact acagccagta aagacaatca
     1921 gaccgtcttc ttgctcaatt acgaccaata attcatctga aaaatcatca taaacaaatt
     1981 tatcatccat ctttttgtat aaattcttgg tggtcgagtt tgctttgcct agggggatga
     2041 tatggaagct cggagcaagt tctgttttcg tttttattgt atgtaaattc tcatgcattt
     2101 taaggatttc atttttagga ataccaacat acagaaacgt ggttaaaatc tttaaataat
     2161 ggggtaaagt tacaccttca gctgtataaa taggggcgtt tcctgatttt aaatatctta
     2221 acccgccggt gtggtcagaa tgaccgtgtg aaataatcac tgcctccaga tcattcaaag
     2281 tttcccccat tttagtcgca ttatagacta attttttatt agcgccagca tcgaagagat
     2341 atttcttccc tgagtagtta attaagagag ataacccatg tccactttta aagttcaaat
     2401 ttattcttga gttttcaaat aaaaccttga tttccactat aacatctcct ttcacatgaa
     2461 cgtgtattca tatattgtag cacattaagt tctatttttt gttgtaatta ccgaaaaaaa
     2521 tattaatgga cgctttttac gctttatttc gtatgtggaa gaaaatttct taggatgtgg
     2581 cgggggattg gggcaaagct aaattccaat tacagtgatc atttaattag acacctttaa
     2641 atacccacac tttcatcaac actataattt agggaaagaa aagtgttggc atgatataga
     2701 aaaaccttta attaacagcg tttcctaaaa ttggcacgca tcttgcttat agaaaagcgt
     2761 aaggatagga ggaatgaaaa tggcaactat aattcttgcc agccatgggg aatttgctca
     2821 aggattaaag caaacagcga caatgattat tggagatagt gtaccaattc atgccttgtc
     2881 agcatttaga gatgaagatg aatctatttt ggtacaaata aaaaaacttt tagccacaat
     2941 agatatagaa gaaacctaca ttttaactga tattctaggt ggtagtgtga ataatgatat
     3001 gctcactatt attaaagagt atgagggatt gcaattaatt actggaatga acttaccttt
     3061 agttatttct attgcaacct ataatggaaa aattggggaa agtgagctag aaaagcttat
     3121 taaagagagt caacaatcag tgattgattg tagaaaatta ttagtagaac aacttactgg
     3181 aggagatgac ctatgattaa gttagtacgt attgaccatc gtttgttgca cggacaggta
     3241 gtattttctt ggagtaaatc attaaatatc aatcgtatta ttattgccaa taaagaggca
     3301 gcgaatgatg aattcaaaaa aatgtctttg aatttatcta aaccgactgg cattaaatta
     3361 acaatttttt cagttgaaca gttactagaa aaaatgccta aaatcgaaac acttaacgaa
     3421 aatatcatgc ttatttttgg taatacaaaa gaaattcgtc aattttgtga agcctatcct
     3481 aaagtacaag aaatcaacta tggagggata atcaaaaaag aaggttccaa gcaatttagt
     3541 aatgccattt tcttgaatga atctgagcaa gaagatgcca aaaaattaaa agctcttggc
     3601 attaaacaat ttattcaaca ggtaccaact tctaaaaaag aagatttgaa tacaatgtta
     3661 taaaggagga atgaaaatgc tcgttcagtc aattggttta gggttaatcg gtgttttttg
     3721 tattttagac tcaaggcttt taggaagaat gaattttgaa cgaccgttga ttgtcagtac
     3781 gcttgttggt ttggtacttg gagacatgga aaaaggatta attgttggag cctctttgga
     3841 attgatgtcc ttaggattag taaatatcgg agctgcttca ccacctgata tgaatatggc
     3901 atcaattatt gctacagcct ttgccatttt atcaaatgcg aatgcagaag cagcattaac
     3961 gattgccatt ccaatttcag tgttaggtca aatactaggt atcttaatgc ggacgatttt
     4021 gtcaaatttg actcacgttg cggatcgctt tatcgaacaa ggaaaattta gccaagcaag
     4081 gagaattcat atactttatg gctcgggatt atatgctctc atgtatttta ttcctatttt
     4141 tctagcaatt tattttggaa cgaatcttgt ttctcagatt gttgatgcga ttcctgagtg
     4201 gctaacaaat ggcttaacgg ttgcaagtaa aattttacca gcatatgggt ttgcgttgtt
     4261 attacaaacg atgttaacga aaaaaatggt gccattttta ttacttggat ttttaattac
     4321 tgcttattct ggattgggaa tcattggtat tgcagccttc gcatgtattg ttgcgtttgt
     4381 catggttcaa gttcaaggta aaaactcaaa tggtaaccaa aatgagccag atccattaga
     4441 tgacttataa ggaggtagat taagtgaaag agaaagatca aaatgcatgg aaatatcggc
     4501 agttcttatg gcgttcatgg gcaattcaag cctcatggaa ctatgaacga caaatgaaca
     4561 tgggttttat gtacggaatt gcaccaattc tagataacat ttacaagaat ccagaagatg
     4621 aagcaatgaa gaaagaagct tacgagcggc atatgatgta ctacaactgt acccctcaaa
     4681 caagtgcatt tgtgttaggg ttaactgctt ctatggagga gcaatactac gaagaccaag
     4741 aaaactttaa tccggaatca atcagtgcag tgaagacttc tctgatggga ccgctatctg
     4801 gtgttggtga ttctttcttt caaggaacga ttcgggtatt agcctttgga ctaggaatta
     4861 atttagccca acaaggtagt attttaggtc caattctagc aatgttaatt tcctttgtac
     4921 cttccttttt agttacttac tatggtggga aaattggcta taacatgggg aataaatact
     4981 tgtctaaact gtatcaagaa ggtttgatgg ataaagtaat gtatgtttgc tccattgtgg
     5041 ggttgatggt cattggttcg atgattgcca gcatgatcgg gattacaaca ccaattcaaa
     5101 tgggtaaagc ttttgtctta caagatgtat tagatagtat tatgccacaa tttttaccat
     5161 taggattaac cttcttcatg tactggttat tgcagaaaaa ggtcaaaaca ggatggatgt
     5221 tggcaatctg tttagtcgga ggaattattt ttagtttgtt aggtatctta caataaggga
     5281 ataaccaata aatttggttg aataatataa ataaggagga attcaattga tgaattcaaa
     5341 agaaattgtt aaagaaatcg tagcgaaaaa agaggtaaaa tcaatcgtat ttgttggctg
     5401 tggcgcttca aaagcggacc tttatcctgc aaagtatttt ctagaccaaa atgcgaagca
     5461 gttacggatt agtcattaca cagccagcga atttaactat gcaacaccag catctgtaga
     5521 tgagtcaact gtcgttattt cagcttcttt aggaggtacg actccagaaa cagttgaagc
     5581 taacgcagta gctaaagaaa aaggtgctac agttattagt ctaacacgaa gcattgactc
     5641 accgttaaca aaagatgcag attatgtaat ttatcatcga tttgcagaga actatgctgc
     5701 taaattagaa aaaacaggct actgtttaga attggcagta gaacttttac atcaagtaga
     5761 aggttatgac cattatgaag caatgcaaga aggatttgct actatctatg atcttgccca
     5821 aaaaagtgca gaatcagctc gtaaagatgc gaagaaattt gctgaacgct ttaaagatga
     5881 aaaagttatt tatgtaatga gtagtggtgc tacacatgaa gtagcatact caacatcaat
     5941 ttgcttgatg atggaaatgc aatggattaa ttctggtacc ttccattcag gagaattttt
     6001 ccacggacca ttcgaaattg tggataaaga cgtaccgttt atattattaa tgaatgatgg
     6061 aaaaactaga aatatagatg ctcgagcatt aactttcctt gaacgctttg atacgaaaac
     6121 agaagttgta gatgccttgg attatggctt gtcttcacat attgctaaag aagtcttaga
     6181 ctactttaac ccattcgtga ttactgcagt atttagagtt tatgcagaag aattgtcaga
     6241 agcaagagaa catccattaa ctaagagacg ttatatgtgg aaactaactt attaaaaaat
     6301 agatcaaact aaataaggtg gcttgagaaa ttaagccacc ttgttttctt caatgtgatt
     6361 ttatacatat ggtaaaataa ctagtacata tggtaaaata actagtacat attgtgaaag
     6421 caggtggcaa gatgcagcga ttaaatgact atctttatca actaacattg cttttgaaat
     6481 ctacgccttc agataattat tcagctcgca gtatttcgca agctctacag ttgaatagaa
     6541 gtacaatcag tagctactta aatgaaggtg ttcgtgaagg gcttttcata aaagtaaaaa
     6601 gttacccggt tttattttta catagaacag ctcttgaaga actacatatc acactgaata
     6661 actccgaaat agaatcaatt gaaagcttgt taacaatttc tcaaaaaccc gcattagatc
     6721 aagtaattgg ctctaaagga agtttgaaag aagcaattga tcaaattaaa acggcagtct
     6781 tatatcctgg aaaagggttg cctttattat tgatcggcgc tagtggatca gggaaaacct
     6841 ttctagcgaa taaaatatat gaatatgcag tagaagaaaa ggtcattcac acatcggcac
     6901 ctttcataga ttataattgt gcacaatatg tgagcaatcc agagttacta tcctctgctt
     6961 tatttggcta cacgaaagga gcatttaccg gagcaagcca ggagcatact ggtttgctag
     7021 aaaaagccga tggaggagta ttgtttttag atgaagtgca tcgcttatct gaagaagggc
     7081 aagagaaatt atttacattt atggatacgg gtgaattctc tcctatgggg gataatagta
     7141 ttcggaaaaa agccgatgtt cgattagtct ttgcaacaac agaaaatata tatacgactt
     7201 tcttaccaac ttttcttagg agattgccag ttattgttaa tctacctaga tttcaacaac
     7261 gtccgtcgtt tgaacgtctg tcattgattg atgaattttt tgtttctgag agccaaattt
     7321 tagctaagga actatctgtt tctgatgcat tgattcattt cttaatgaat agtgattttg
     7381 aaggtaacgt ggggaatatt aaaaatatta tcaagtatgc ttgcggaaac gcatatatcc
     7441 accagaagag tcagcaaaca attcaagtta aattattgga tatgccttta gagtacagtt
     7501 ataaattcaa ggagcaacta aataaaccca gaaagaaaag agcagatcga atctactatc
     7561 cagaagaaac acgacaagtg caactagaaa gaaacaatca gctttttgtt caattttttg
     7621 accaattact tactggattc tctgaagtga ttgaaaataa caaggatatt aaactatttt
     7681 tagaagaaat ggttaccaag gtaacacaag taatggatac gcttattttt caagaggatt
     7741 acgaaaaaga gcagtcttta tacgccattc taaactatca tattcgtcaa agtattgatt
     7801 ttatgcgaga aagctacggg tttgaacaag atggtaaccg ggtagtcgct ttggccaact
     7861 atctatatgt caaggataat caagtgattt ttgaaaatag tcttcaagaa aagaaaacca
     7921 aagatcagct taacgaattt tttgctaatg ttcttgaaac cccttattgg tatgcaaaaa
     7981 aaatcttaag ccttttatct gagtgtttag atcaacctgt gtatgaagag gacattttct
     8041 ttattacgct atatttttat agtttgaatg tcacgaatgt taaaacaggt attaagagta
     8101 ttgtgctagc tcatggttat tctacagcta gtagtatggc gaatgttgcc aaccgtatgc
     8161 tgaataagaa cgtttttcaa gcttatgata tgcctttaga tattacatta gagaaaattg
     8221 aagcaaaatt gattcaattc cttggagatt atcgaacaga ttcaggcttg attttattgg
     8281 tagatatggg ttcactaaac caactaggcg gtttattagt gaatcatcta aatggaccac
     8341 tgttaatcat tgatcatatc aatacaccgt tattattgga agtcggccaa caagtattaa
     8401 acaataaaag tatcaccgaa ataggagcag aaattgaaac aagtcttact ttcaaaaagc
     8461 agttactact tcccaagaaa aagaaaaaga aagccatcat tacttgttgt tatacaggga
     8521 ttggtagtgc gacccagatt caggagattt taacgaaatg cttgaaagat gcagttagag
     8581 aattagtaat tattccgtat gattacaaaa agctgttaga aaacaagaat tttgagactc
     8641 cttttcaatt atacgatgtc atcatgattg taggtacaga agatcccaaa attaatcaga
     8701 ttccatatat tggattagat cgcctgatta atgggggaag cattggcgaa tttgttcatt
     8761 acttggaaaa agagctgact attgaagtga acgatttaca aaaagaatta attttcaatt
     8821 tttcaattag taaaattgtc gaaaatttaa ccattttgga tccagataag gtgctatctt
     8881 ttgtacaaaa agcaattaaa gaattatcga agttattaaa ttttgatttt tctaataatc
     8941 agctgttttt gttgtacctt cactgtagtt gtatgattga acgaattgta cgtaaagaaa
     9001 gtgtggatga gcaagaggac attgaagaat atatgcaagc aaatggagaa ataatggata
     9061 aaattcatag cgcttttttt gagattgaaa gggaatacac aattgaaata ccagatttag
     9121 agttgcgatt attgagcgat attgttcaag gataggagtg aattcgaatg acaatgggag
     9181 taatttttga tatggatggg gtactcatag atagcgagac attttatttt gagagaagaa
     9241 tgaacttctt tcaagaactg gagatagatc caggatcaaa ccagattgat gattatgtgg
     9301 ggaaaacaga agctgcaata tgggaaacat tagttcctga ggacaaatca ttaagagagg
     9361 aactttatcc gaaatatgta gattatcgaa aaacgcatcc aattgatttc gctaaagctt
     9421 tacgaaaaga agtgaaaccg ttacttgact tcctcaagaa gcgggatata ccattggcac
     9481 tggcatcttc ttctccagct gtcgagatag agagtatgct cattcaatgt gaacttactg
     9541 aatatttttc ctttgttata agtggtgagt cattaaaaca aagcaaacca catcccgaaa
     9601 tatatttaaa agcgaaacag caattagact gtgacttctt tattgcagta gaggattcaa
     9661 cgattggtat taaatcagca aaggcagcaa atatctatac ggcggcacta aaacaaccaa
     9721 tgcaaatcga tcagagtgaa gcagacatac aaattgaaag tctacaagaa ctggaacaaa
     9781 ttgttttaga aaaaggttag tgaattaatg gaaataaggc tatatatctg tgctagctat
     9841 tttgactaac caaaaaaact tgctataatt caggtgtagt aaaaggtgct ggtaacacct
     9901 tttacgaaaa tgcttcggca gtgataatgg gattggcttt ttaagttttt tattttgttc
     9961 gcaatcccta aggtcatgtg aatgatccta gggattgcta gaatattttg caacagaacc
    10021 aaaatacgaa tagttatcaa gcagccatat tgactgcggt caatttagga ggggatactg
    10081 atactactgc tgccgtaact ggtgctcttg cgggtattct ttacgggggt gaagtgatcc
    10141 ctgaaaattg gagaaaatcg cttcaaaatg accagcttct taaaacgatc agtcagagat
    10201 tttgcgatac cgtgctatct caaagaattt gaataatgga tatgatgcaa atcttgtttg
    10261 tactaacgtt gtattgacaa aaaggatttt atttagtaca atgttagtac aaatgatgaa
    10321 cggaggtaga atcatgtctc aatcaaccat tacatttagg attcccgacg atgaaaaaga
    10381 actggtatta gaatatgcaa aagcacacaa ttcttcattg accgaacttt atcgaaatgc
    10441 tgtactggat aaaatcgaag atgaaatcga tctacggact ttacggagtg caattgaagt
    10501 ttccaaagag aaaaaagaga gtggtatttc tcaagaggaa atggagaaac tgctaaatga
    10561 attataaagt catctacatg ccgcaagccc agaagcaatt aaaaaaaatg gacagaaacc
    10621 aagctaaaat aattgtcgcc tggatcagaa agaatttaga aaacacgact gacccgcgtc
    10681 agcacggaaa aggtttgact ggaaataaga gtggggaatg gcgctatcga attggaaact
    10741 atcgaatatt ggctaatatt tgtgattctg aaattgaaat ccagatattc tcaattggac
    10801 atagaagtac aatatataaa agataaccgt ttttggaacg aagtagagtt tcgaaagtca
    10861 atgtataatc ttttgaattt tgcataatac cacaggtaga aactacccga tactatcggt
    10921 tggtttctgc tttttta
//
LOCUS       DAPBCS010000074        10596 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPBCS010000074 DAPBCS010000000
VERSION     DAPBCS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.077"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            30..2108
                     /locus_tag="RD521_002567"
     CDS             30..2108
                     /locus_tag="RD521_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDT7772240.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2268..4154
                     /locus_tag="RD521_002568"
     CDS             2268..4154
                     /locus_tag="RD521_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDT7772241.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4166..5113
                     /locus_tag="RD521_002569"
     CDS             4166..5113
                     /locus_tag="RD521_002569"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDT7772242.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5133..5660
                     /locus_tag="RD521_002570"
     CDS             5133..5660
                     /locus_tag="RD521_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDT7772243.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5723..6376)
                     /locus_tag="RD521_002571"
     CDS             complement(5723..6376)
                     /locus_tag="RD521_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDT7772244.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6508..7350
                     /locus_tag="RD521_002572"
     CDS             6508..7350
                     /locus_tag="RD521_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDT7772245.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            7508..8404
                     /locus_tag="RD521_002573"
     CDS             7508..8404
                     /locus_tag="RD521_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDT7772246.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8407..9120
                     /locus_tag="RD521_002574"
     CDS             8407..9120
                     /locus_tag="RD521_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDT7772247.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9140..9658
                     /gene="msrA"
                     /locus_tag="RD521_002575"
     CDS             9140..9658
                     /gene="msrA"
                     /locus_tag="RD521_002575"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDT7772248.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            9655..9882
                     /locus_tag="RD521_002576"
     CDS             9655..9882
                     /locus_tag="RD521_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDT7772249.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10034..10585
                     /gene="lepB"
                     /locus_tag="RD521_002577"
     CDS             10034..10585
                     /gene="lepB"
                     /locus_tag="RD521_002577"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDT7772250.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
BASE COUNT         3670 a         1808 c         2186 g         2932 t
ORIGIN      
        1 cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt attgtatttg
       61 cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt ccagctccac
      121 tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa tcaatcgatt
      181 ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga gaaggagaaa
      241 aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg gcatttggag
      301 gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg ccaagtgtcc
      361 cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg gtagcagtca
      421 gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg gaaggagaag
      481 gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt gcgctgatca
      541 ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt attgggggag
      601 ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg atcgaaaaag
      661 catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct tttctagcct
      721 atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca gcaggtattc
      781 tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg agcttgtccc
      841 acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata tttttaggaa
      901 tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca aacggtcttt
      961 tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc atttctatca
     1021 gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg aacgagatct
     1081 tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt tttattttgc
     1141 cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt ctgacggcgg
     1201 gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg acggaaacag
     1261 aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc gttgaagttt
     1321 tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc actgaagcag
     1381 tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta accgatgatg
     1441 aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg gatggattgg
     1501 atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac tcccgttttc
     1561 tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc ttttggttca
     1621 ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg caaagaagat
     1681 atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga gagacatatc
     1741 aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt tatgatgaaa
     1801 cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg aactcgggga
     1861 atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa atgctaagag
     1921 gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa atcacgacgt
     1981 tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg atgaacaaac
     2041 catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa acgagtccaa
     2101 taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc agaacaggat
     2161 cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa aaaattttcc
     2221 tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg ttgaaagaat
     2281 taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac cagatttctt
     2341 ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg ggtaaaacaa
     2401 gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa acagtgtttt
     2461 atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc gatgaattaa
     2521 ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt cgagcatatg
     2581 aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa aaacgttatg
     2641 ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc aacgcaaaga
     2701 ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact ttatcaggtg
     2761 gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat ttactattac
     2821 tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa aactatttga
     2881 aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta gatagagtag
     2941 caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga aattatgaag
     3001 cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa gaaaagcgca
     3061 agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca cgtggcacca
     3121 agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac caagtaaacc
     3181 aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa gtactggaaa
     3241 tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta gatttattga
     3301 ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa tcgacattgc
     3361 ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc ggtgaaacgg
     3421 ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa cggatgattg
     3481 cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa atcggagtag
     3541 cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg ataggcaaac
     3601 tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag caacctaatg
     3661 ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg attttggaag
     3721 attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt tatttcttgg
     3781 acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa ctttattacg
     3841 gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct gaaaaagcga
     3901 aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac atggaacaaa
     3961 aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc tcattgctcc
     4021 aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg caaaacgacg
     4081 tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac ttgagtgaat
     4141 gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag gaagaaaatt
     4201 gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa gcttattcgg
     4261 ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga aacgagtacc
     4321 ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga atatccgcta
     4381 tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct acatcaaaag
     4441 tccagactat aatggaccag atatgactga ttttggccat agatgtttga cagatcctga
     4501 attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc taaccgatga
     4561 ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat ggcgtcactg
     4621 ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag caatcaaaaa
     4681 gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg ttcctagcat
     4741 ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac ggctaagctg
     4801 ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca ttgcaagtta
     4861 tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg aatttgttca
     4921 cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag aacaattgag
     4981 ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt cagtatttga
     5041 ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga tcaaagctcc
     5101 tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg gcacaggatg
     5161 aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat gatctgaaat
     5221 tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca ttcgaaggga
     5281 tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat tcttcctata
     5341 aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg gaaaaagaaa
     5401 cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt ctgccatttt
     5461 gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct tattttccac
     5521 ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg gatgaaaaaa
     5581 atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta aaaagcggga
     5641 caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta gagctgtccc
     5701 atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac ataaaacctg
     5761 cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa ttcaagctat
     5821 aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata acaccagtca
     5881 ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc ataaaaatat
     5941 agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac gctttatata
     6001 taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct agccatcctc
     6061 ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa atcgaactat
     6121 gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga aacaaagtag
     6181 acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca atatggaggg
     6241 aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata ctcaatccag
     6301 tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac gttcgattaa
     6361 atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa aaatcattat
     6421 ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga ataaggcatc
     6481 ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac agattcttca
     6541 tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc gctatccatc
     6601 atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag atttatggaa
     6661 atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg agaattcatc
     6721 tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca tatgacaaaa
     6781 ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa aacgaaagta
     6841 acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat tcgagcagct
     6901 gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa aaaagtacaa
     6961 gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa aggtggaaga
     7021 atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt gatgaatttc
     7081 gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt cgataaatgg
     7141 tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat cggtatttca
     7201 catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga attattcccg
     7261 gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac tggtaaagac
     7321 gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct cagcctgaaa
     7381 ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt ttcttttttc
     7441 ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga atacaagagg
     7501 tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat tattaattgc
     7561 gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac taatgaaacc
     7621 agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca tagctatcgg
     7681 agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg ttccccttgt
     7741 cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa actatggtgt
     7801 aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag aaattcgaaa
     7861 caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt tgatgaaagt
     7921 tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac tgaaaaacta
     7981 tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa ccgcacctat
     8041 ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca ctgacatgca
     8101 gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa tgccgaactg
     8161 ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag taggtgtcac
     8221 agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca tcaaaaataa
     8281 tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat tgatggcaaa
     8341 tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg aggatgatca
     8401 ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac aaattactgg
     8461 aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt tttaggcagc
     8521 cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac ggaaagacaa
     8581 ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat catcagcttt
     8641 ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt ggaaaatgaa
     8701 gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta tttatatttt
     8761 gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct ttctatcgga
     8821 acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta taaattacca
     8881 gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga tcaattccga
     8941 atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga tgacattcgg
     9001 tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa taccaacaaa
     9061 actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc ggatgagtag
     9121 tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct tctggtgtat
     9181 gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat atactggagg
     9241 gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac atacagaagc
     9301 agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag aactttactg
     9361 gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg acaattatcg
     9421 cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa gtaagaaaca
     9481 gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac cagcccaacc
     9541 attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg aaaattttaa
     9601 aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg aagggtgaat
     9661 cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga tgctttatct
     9721 gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc tagctacgaa
     9781 gaaatttcta attatctgga actgaatgtc gactatttgc cgtccatgga tatctttgac
     9841 gaagcatggg aaaaatattt agaaaataat catgacatat aagagatagt tttgtacttt
     9901 atgatcaaaa aagttttttg tccaatagcc taattgaaag aattattttt atttcagcta
     9961 tctagtcgca tttttttgat atttttctta taataaagaa agaatgaaaa aaagagggat
    10021 ggattgatag agattgacaa aaaaacaacg ctatattaat cgcttctggc ttgtattcaa
    10081 atacttactt gtttctgtat tccttgcgtt catgttgcgg ggatttttat tcatcccggt
    10141 accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg ttgtgatgga
    10201 aaaattttcg gagatcagac gatttgatat tgttgttttt caattagcag atggcacgat
    10261 ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa atgatcaatt
    10321 gaagatcaat gggaaagtag ttaaggaacc ttatctaact aaaaacctga aatcagatca
    10381 tgcaaatgca tcctatacga cggactttac actacaagag ctaacgggac aatccaagct
    10441 tccggaagat aattattttg tacttggaga taatcgaaga gtttcaaaag atagtcgttc
    10501 gtttggcacg atcaataaaa ctgatattct aggtaaagca agatttgtct attatccact
    10561 tgatgaaata aaatggatcc agtgagaaac aataaa
//
LOCUS       DAPBCS010000075        10541 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPBCS010000075 DAPBCS010000000
VERSION     DAPBCS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.078"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(48..1514)
                     /locus_tag="RD521_002578"
     CDS             complement(48..1514)
                     /locus_tag="RD521_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDT7772251.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="RD521_002579"
     CDS             1901..2497
                     /locus_tag="RD521_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7772252.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="RD521_002580"
     CDS             complement(2555..3037)
                     /locus_tag="RD521_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772253.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="RD521_002581"
     CDS             complement(3143..3340)
                     /locus_tag="RD521_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772254.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="RD521_002582"
     CDS             3635..4393
                     /locus_tag="RD521_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7772255.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="RD521_002583"
     CDS             4453..4881
                     /locus_tag="RD521_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772256.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="RD521_002584"
     tRNA            4927..4999
                     /locus_tag="RD521_002584"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="RD521_002585"
     CDS             complement(5149..5520)
                     /locus_tag="RD521_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDT7772257.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="RD521_002586"
     CDS             5735..5920
                     /locus_tag="RD521_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772258.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="RD521_002587"
     CDS             complement(6003..6191)
                     /locus_tag="RD521_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772259.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="RD521_002588"
     CDS             complement(6215..6478)
                     /locus_tag="RD521_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772260.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            complement(6466..6609)
                     /locus_tag="RD521_002589"
     CDS             complement(6466..6609)
                     /locus_tag="RD521_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772261.1"
                     /translation="MIQNNTLCEMAQAVLWIYPVKSVSILKLEEKLAIIKVKEGVGFS
                     WIM"
     gene            6636..6893
                     /locus_tag="RD521_002590"
     CDS             6636..6893
                     /locus_tag="RD521_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDT7772262.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="RD521_002591"
     CDS             complement(6964..7155)
                     /locus_tag="RD521_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772263.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="RD521_002592"
     CDS             complement(7296..7616)
                     /locus_tag="RD521_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772264.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="RD521_002593"
     CDS             complement(7639..8073)
                     /locus_tag="RD521_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDT7772265.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8408..9046
                     /locus_tag="RD521_002594"
     CDS             8408..9046
                     /locus_tag="RD521_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDT7772266.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9370..9453)
                     /locus_tag="RD521_002595"
     tRNA            complement(9370..9453)
                     /locus_tag="RD521_002595"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9417..9419),aa:Leu,seq:cag)
     gene            9981..10481
                     /locus_tag="RD521_002596"
     CDS             9981..10481
                     /locus_tag="RD521_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7772267.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
BASE COUNT         3352 a         2063 c         1693 g         3433 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acgtggattt gtcataaatt gtcatgcttc ttttacctat gaaatatttt
     8341 cacgtgttaa agtattcctc gttgaacaca acaaataaaa ctttttaacg aataaaggag
     8401 atttttcatg acatcactta aaactttact ttttggaaca actttggctg ccggcgctgc
     8461 attcttcatg ggaacgactg ctcatgcgga cgaagcttat accgttcaat caggtgatac
     8521 tctatcaacg atttctcaaa aatacgttgg tgataactcc ttgatcaatg ccattgcaga
     8581 atcaaattca atttctgata ttaacctgat ctactcagga caacaattaa caatcccaac
     8641 agaaggctct gcacaagcag ctgctgaacc tcaagcagcc gttcaagaag caccagtcca
     8701 agctgaacct gttcaagctg aacaacctgt tgtacaagaa acggttcaaa cggaaacaca
     8761 agcagcgcct gttgctgaaa cacaacctgc tcctgctgta acagaaactg ctgctactcc
     8821 agcaagtaca agctctgcaa aagaatggat cgcacaaaaa gaatcaagcg gttcttacac
     8881 agcaacgaac ggacgttaca tcggccgtta ccaattagat tcttcttact taaatggtga
     8941 ctactctgct gcaaaccaag aaagagtagc agaacaatat gttacttcac gttatggttc
     9001 ttgggaagcg gcgaaagcat tctgggaagc aaacggttgg tactaagatt cattatttga
     9061 acgtttaaac acattcaaaa tatagtaaga ggtcatgcga tttattcttt cgcatgacct
     9121 ctttttgagt ttaagtttag tctttattcc ttaactatcg tttgccagta tttcttgtat
     9181 cagaatctat aaatctacta tttgaatcaa tctattaggt tttaaactgt cccaccttca
     9241 cacaaatttt ttttgattta tttgaatctg gttccgttgt ttaaaatttt tcctatattc
     9301 ttttagataa tatgttattt tctttctcat cgctccaggt gtaccttctc tttataaaaa
     9361 aacaaattat gcggctaaga agactcgaac ttccacgagg tcaccctcac tagcgcctga
     9421 agctagcgcg tctgccaatt ccgccatagc cgcatcacat cttttagttt aggattacat
     9481 gtgttttttg tcaagaacat atttttgttc tttttcattt ctgtacgccc tcccttatgc
     9541 catctacaca aataattcat ccgagcaact ccatcatcta ttgagggttg gtgaaaaaat
     9601 ctgttttatc catatgatcg atttcaatgt taaacgaacg actctgggat tagtaactat
     9661 tttatttttt tcttcatttc ttttttcctc tttttctcga ttcagctatc ttctttcatt
     9721 tggatattga ttatttcttc tgttgattac tatccttctg gaattctaga cgctgaagta
     9781 attcacgttt tctttcttct agagatcata agtaacgaat gaacgaactt caaataattt
     9841 ttttattgta attcatattt gtattgttcg atgattctta atacgatatg acacaataaa
     9901 taaaaatagg catggctatt tacaacaata gtgctatagt agaaaacaaa tgaataaggt
     9961 tacttaaaga ggtgaatacg atgaaactga tcaaactatg ggaagcagat ttaaagaaag
    10021 cttatcagct acaaactagc tttggaccaa atgagagtgg atttatcaac aatgcttatg
    10081 gctatacatt tgctgaattt ttagattatg taaaaaaatg cgaaaaaacg tctttaggaa
    10141 tcgatcttcc tgagagttac gttcccgcaa ccgtctttat tttagaaagt gatgaacatg
    10201 agtatgtagg aatttttaat ttgcgccata aactgaacga aacattaaga aacggcgctg
    10261 gccatattgg ttacggcatc tcccctaatt atcgtaaaaa aggatatgcc acacaaggat
    10321 tagcattagt actacaagag gcaaaaaaaa tcggcattga tgaggcactt ttgtctgtaa
    10381 ataaaaaaaa tcttggcagt cttaaagcac aacaaaaaaa tggggctgcg atttatcgtg
    10441 aagatgagaa agaatactat acgaaaatca aactgatgta aaaaagaagg attccaaaag
    10501 gaaagggatt tgaatgtcct ttatcaatag aacacattta t
//
LOCUS       DAPBCS010000076        10347 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPBCS010000076 DAPBCS010000000
VERSION     DAPBCS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.079"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..268
                     /locus_tag="RD521_002597"
                     /pseudo
     CDS             1..268
                     /locus_tag="RD521_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751573.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
     gene            436..1395
                     /locus_tag="RD521_002598"
     CDS             436..1395
                     /locus_tag="RD521_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7772268.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1456..1665
                     /locus_tag="RD521_002599"
     CDS             1456..1665
                     /locus_tag="RD521_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772269.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            1872..2072
                     /locus_tag="RD521_002600"
                     /pseudo
     CDS             1872..2072
                     /locus_tag="RD521_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2784..3389)
                     /locus_tag="RD521_002601"
     CDS             complement(2784..3389)
                     /locus_tag="RD521_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772270.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3571..3906
                     /locus_tag="RD521_002602"
                     /pseudo
     CDS             3571..3906
                     /locus_tag="RD521_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            4071..4298
                     /locus_tag="RD521_002603"
                     /pseudo
     CDS             4071..4298
                     /locus_tag="RD521_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4424..5395
                     /locus_tag="RD521_002604"
     CDS             4424..5395
                     /locus_tag="RD521_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7772271.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5535..6848
                     /locus_tag="RD521_002605"
     CDS             5535..6848
                     /locus_tag="RD521_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDT7772272.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6891..7757
                     /locus_tag="RD521_002606"
     CDS             6891..7757
                     /locus_tag="RD521_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDT7772273.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            7754..8602
                     /locus_tag="RD521_002607"
     CDS             7754..8602
                     /locus_tag="RD521_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDT7772274.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8616..10088
                     /locus_tag="RD521_002608"
     CDS             8616..10088
                     /locus_tag="RD521_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDT7772275.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            10105..>10347
                     /locus_tag="RD521_002609"
     CDS             10105..>10347
                     /locus_tag="RD521_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7772276.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3323 a         1743 c         2089 g         3192 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tcttatttca aaattaatca atctgttgct aaaactgccc attgcttgaa tcgttcaaga
      541 caaacgatcc ataaagtata tctgttcttc aagcaaggaa aatcagcctt agaatattat
      601 caacagtata agaaaaacaa atcaaactgt ggtagacgtc cgcttgtttt acccgaggaa
      661 caatcagaat atattcaaag aaaggttgtt caaggatgga cacccgatgt gattgttggt
      721 cgtgcagcgt ttcctattag ttgttctgct cgtaccattt atcgtatgtt caaaaagggg
      781 ctatttgatt cttctgactt accgatgaaa ggcaagcgta aaccgaatgg acatcaagaa
      841 agacgtggaa aacaaacttt ccgccgctct attcatgaac gtgaaaagga ttatagccaa
      901 ttctcaaatg agtttggtca ccttgaaggt gacactatcg taggtctgaa acataaaagt
      961 gctgtaatta ccttagttga acgattatca aaagttatca tcacattgaa accgtgtggt
     1021 agacaagcga ttgatattga aaaaaaatta aatcaatggt ttgaatctgt accgaaaaac
     1081 ctattcaaat ccatcacttt tgattgtgga aaggaatttt caaattggaa acagatcagt
     1141 aatgtcaatg atattgccat ttatttcgct gatccaggaa cgccgtctca aagaggccta
     1201 aacgagaatt ctaacggatt gttacgtaga gatggtttat tgaaatctat ggatttcaat
     1261 tcagtagatg aattttttat tcaatctgtc gcatctaaac gaaataatat tcctagaaaa
     1321 tcactggatt atcgaacacc tttggaagta tttttgagtt acgtaagtat tgatgatctg
     1381 tctaacttaa tttgacaatt aagacattta aaaagaaaag tgacagatag gaataaaaac
     1441 ttattaaatg tagttatgat ctgttatatt tcatttctaa tactaggtct tccacaagca
     1501 gtgtttcctg aaatgttttc tggagactgg ggtattgtca gctattctat attaacattg
     1561 attgctatgg ttttaggtac ttcattttat aatgcttttg taagtaagca tgttgaggaa
     1621 tatgaagtat taatgtactt aaaagagaaa aaaacaagta attaatatga gaacaccacg
     1681 catagttttt attttgagat attctagaat gaaacacatt aagaacgaaa gcaaagcaaa
     1741 agacgccaga acttctacta ccttctaagt tttttcatat ttgtttgtta tgctatacat
     1801 agatctgcat aattagactt gcgctgcccg ttattgaagt ttcaggcagc gctctttttg
     1861 ggggatcgat aatggaagaa gaaaaaatat ttgagaaacg ctggcagcta gcatcaagtg
     1921 aacaaagagc aagatacaat aatttgatca cctcctaccc cactgttgac tagacatata
     1981 aggaaaagaa atacttactt tggctgtgcc agttagatat agataccttt gaaacatttg
     2041 aagtgatcct agataaaatt aaacagaatt gatcagaaac aagcgaagcc ttctctcggt
     2101 tcgcttgttt tgctatttat tagttggctc agggcaactc taagctcgtt tgaaagaaaa
     2161 ccgatggata atccccttgt ttgtaccact ttagaataca tggatgtacg tattgccatt
     2221 gactcctatt agttactgaa aaaaaattta aacaagttga gtattgcaga taaatagctc
     2281 cagtgaggtg aataactgct ccactcaagg caatagatag ctccatccag ggcaacaaac
     2341 tgctccactt tcatatttaa tagatgaata accttctgta taatagaaag gcataccttt
     2401 cggtatgaca acttatacag aatgttattt tattggttca ctatcgaaaa attttggagc
     2461 tttatttttc cggtatttct caacgaacga ttagtttaac tgtgggaagc tccagacata
     2521 cgattcgaga agttacttct cgagcagaca ggaagggggt gactgaacta accgctgata
     2581 tgagcgatac ttggctgtag gaatatcttt ttccggaaaa acaaacaaca gcacgaggtt
     2641 accttcctgt agactgggaa tatgttcata aagaactaat gaaaaagaat atgacattga
     2701 aactccttca tagagaatat ggggtacatg gaacatagaa acgaaaatgc acggtagaaa
     2761 agcgaatgaa tatagtataa attctacaaa tcattcgctt tctttatttt atatatggtt
     2821 ggtcgagaga gaccattttc tttggcaatt tctgcaactg gaactccttg atttaacatg
     2881 tccaccacag ttttaaagac taatcgtttt tgcggatctg ttgagttctc agaatattca
     2941 attggtcgcc ccttatatcg accagctttt ttggccaata atactccttg ccgctgacgt
     3001 tcacggattt tctcacgttc gttttgtgca atataactta gcaatgataa aaacatatcg
     3061 aacatagcag tatctaggag ttcatttcca gtattaaaat ctaaaaactg tgcatctagt
     3121 atttttaatg ctactttttt ctgtttcata aaggtaactg tattttttat gtcttcataa
     3181 tctctgccta ggcgatccag tgatgcaaca atgacacaat caccttctcg gatataattt
     3241 agtaattttt gaaactcagg tcgctggata tctttaccac tggctttttc ttggaataat
     3301 ttatcaatgt taaggtcttc catcattgcc aattgtcgat ctaaattttg atcttgtgaa
     3361 gacactctaa tatatccaat ttttgccata aaaactcctt ttaaaaaatg atataattta
     3421 catgtaaaat ttacttgtag ttttactgtt atcatagcat ttttaaaaat aaattttaag
     3481 tctacttata atttactttg gaggttttga atgaatgaaa ttaagcgcaa taaaaattct
     3541 tttggtgttt atttaggaga gcctagctcg gaacagttaa ataaaggtga agctcgtcat
     3601 gctctatgtc gaaatatatt ttatggtaga aaaggcaaac tttatcaaac ttattacgac
     3661 ggtatggagg aacagctcaa cgcattagga ttagtaacta atgctatcat atattggaat
     3721 tcactatatt tagaaaaagt ctttgaacaa attaaaacgg aagggttcga ctgttccgaa
     3781 gaagtgttac gcaagcaatt tccattaatt agtgatcaca ttaattttgt tggcaaatat
     3841 agtttccgct atgaaaaaga attatcgaat ggaatactac gtccgttaaa tatagagaat
     3901 tattgagaat gttcgtcttt tggttcctac cgtgcatttt cgcacggatg ttccagatac
     3961 tatttttgaa tgtataaaat tgaaaacaca agaatactaa taaatcaacg attcttgtgt
     4021 ttttaatttt tcactaccgt gcattttcgt ttctatgttc catgtacccc aatcttgaaa
     4081 aattaattaa agacttagat aaagccattg acgacttagt cgtggtgcta ccggagtatc
     4141 aatgtttaac cagtatccca ggtattggtc ctgtttaggc tgctggaata ctcgctgaaa
     4201 ttggtccaat tgaacgtttt tctgatgaaa taaaattgac aaagtatgcc ggcctatatt
     4261 ggcgacagag tcaatccggt aaatctgaat acgaaaattt ttttacgaaa agcttcacgt
     4321 ctaatatcta ttttaaaata aaaatattcc tgctaatttg gaatcgtttc cttcttttgg
     4381 tataataata cctgaaattc ttttgagagg agttgttcgt gaagtgaaaa ctactatgaa
     4441 ggatgttgct gcgttagcag gagttggtgt gggtacggta tctagagtaa taaatggagt
     4501 aaaggtgaaa gaggttacca aagaaaaagt aatggctgca attagtgagc ttaattatga
     4561 gcctgatgaa tatgccagag gtatgaaaac gaatagtagc aatacaatag ctcttatttt
     4621 acctacgatt tggcatccct tcttttccga gtttgcctac tatgtagagg aagctctcag
     4681 ccaaaaaaat tataagcttt ttttgtgtaa tgcgaacggc agtgcggaaa aagaaaaaga
     4741 atatatccaa atggtcaagc agagtaaagt agacggaatt ataggtatta cctattcaga
     4801 cattgataaa tatgtatcat caaatttacc atttgtcagt atagaccgcc atttttcgga
     4861 acaggtttct tatgtaacgg cagataatta tttaggtggg caaattgccg ctcaagaact
     4921 acttactcgt aactgtaaaa acttgtgcta tgttgggggt gtttcacctt accctaacga
     4981 aacaaacaat cgtaaagctg gtttttacaa tatttgtgag gaaaataaaa tagctgtgaa
     5041 ggtattggat atgcctgaac caattagcaa tttagaggaa caattgctcg cttttttttc
     5101 ggataatcct gaaattgata gcatttttac catcaatgat ttcatggctt tggatgtgat
     5161 aaaagcccaa aaaaaactag ggaaggaagc acctaaggat tttcaaatta ttggttttga
     5221 tggagtcaag ctatcgttgc aaaaagacta tttactatca acgattgtac agccgataga
     5281 ggaaatggcc aaggtttcag tagaaacact catggcgatg attttaaatg aagaaccgcc
     5341 taagcgtgtg gtagtaccgg ttttctttca cgctggagag acgactaaag agtaaaaaac
     5401 cggtttataa cgattttttt gagataggtt gacagaaagt gatttcaatg atatcttaaa
     5461 accaattaat tggaaacgtt accaattaat tgaaaaataa aacggaaacg tttcaaaaaa
     5521 taggaggaac atcgatgaaa aagagaatgt tagcaattgc agcaatttta gcattaacag
     5581 ctggaggact tgcaggatgt ggcagtaagg agaaaagttc aggtagcagt gatccaggag
     5641 aaattaagat ttgggttcag ttttctgatg aaacgcctga aggaaaagct tgggcagaga
     5701 ttgtgaacaa ttttaatgat gaatacgagg gtgaatataa agctgttact gagtatattc
     5761 ctcgtagcgg aagcggcggt ggatatgagg ataaagtaaa tgctgcaatt acaaccaata
     5821 cccttcctga tgttattacg ttagatggac cgaatactgc agcttatgct cattcaggag
     5881 tcattgctcc attagatgat tatttgaaag atgcggacat ggatgatgta ttagacagca
     5941 ttattcagca aggaacctac gatggcaaaa tgtatgcgtt tggtttctca gagtccagtg
     6001 tgggtgtcta ttacaataag tcgctgttca aagatgctgg aattacagaa gatcaattgc
     6061 cgacgttaga agatccttgg acttggacgg aatttcaagc aatttgccaa aagctgactg
     6121 ataaatacaa aacgcctgca attgatatgc aaatggcgtc gggagacgaa atgctgacct
     6181 acgcatatac accatttata tggtcgaatg gcggcgatgt cgttaatgct gatggaacca
     6241 aagccgaaga ctattttaac ggtaaaaaat ccgttgaagc gttggaattt attcaagaat
     6301 tggttgaaaa aggttacacc acaatcagtc ctattgaaaa aggatttgaa actggcaaat
     6361 atgccatgat gctgagcggt tcttggacaa tggcagattt agaggaaaac tatcaagaga
     6421 ttgattatgg tattttgcct taccccgttt cagatgcaac tggggagtta gtttcaccaa
     6481 ccggaagctg gcagttagca atgactgaga aaacagagaa aaaggatgct tctgcggcct
     6541 ttattaagtt tgcaaccagt acagaatcta gtcgtattat gagtttaggg aacagtgttc
     6601 tacctatcag aaattcaaca gttgatttaa ttaaagatga agtgtcggaa ggaatgcagg
     6661 tattaattaa gcaaaaccaa ctttctggac atgcccgccc agtggttgta gcttatccgc
     6721 aagtatctag agcattccaa caagcagttc aagatattag ctacttcgag gaaaacccag
     6781 atgtacaaaa agtagcagat agtcgcgctg ctgaaatgca acaggcaatt gatacttcac
     6841 taaaatagaa gtttctaaag ggggccaatc aagtgttaaa aaaaccgaca atgaaagaca
     6901 atttgatagg ttacggcttt ctagcgccag ctttgctgct gctgacgatt tttctgatca
     6961 tacccgtaat catggtaatt tattatgcct ttactgatta ttatttgtta acgccagatg
     7021 ctagacaatt tatcggctta gaaaatttta agcgactgtt cgctgatcca atttttgtaa
     7081 aaagtatttg gaacacggct aaatttgttg tttggattat tccggtccaa ataggggcag
     7141 ctttaggaat ggcacttatt atcaacaaaa atcgtaaagg aaatatcttt tttaaagtag
     7201 ccttttttgc gccagtcgtg atgtccttag tcgttatttc gattttatgg ttgtacttgt
     7261 taaatcctaa tgaaggtctg gttaatacgc tatttgcaaa gattggaatt gcttcgcagc
     7321 cttttttgac tagtcccaaa caagcaatgt atacgcttgt tttcgtatcg gcatggcaag
     7381 gtgcaggata tcagatgctg ctgtttttag ggggaatgca aaatattccg caagacgttt
     7441 atgaggcagc cgaaattgat ggtttttcta agtttcagca atttcgctat atcaccatgc
     7501 cattattaaa gcccacggcg atttttattt tacttaccac attgatttcg gcctttaaac
     7561 taatcattca gccaatggta atgactcaag gtggaccaat gaattctact atgacaatgg
     7621 tttattacat ttaccaaact ggatttacag accgtatggt aggctactcc agctccattg
     7681 cgttaatctt taccactatg attggtttaa ttacaattgc tcaaagaaaa ttggtaaagg
     7741 aggatgatcc agcatgacca agaaatttgg accgctaatg atcgtagaat attgtttgct
     7801 gttcctttta gcggcattat tcattttccc gatgctgtgg atggtagttt cctctatgaa
     7861 gcctgaagca gatgtctatc aaaatttagc ttccttcaaa gcttttttac ctagtttgaa
     7921 tccagctaat tggtttaaaa cttatcaaga ggttatttcc cggttcagtg tgggaactta
     7981 tttattaaac agtatttttt atggtttaac ctttgcctgt gggtcaatca ttgtaaactc
     8041 tttggcgggt tttgcctttg cgaaggttaa tttttcaggc aagaaaatta tctttggagc
     8101 tttgttagct ctgctgattg tcccgatgga gacggtttta attccgcaat ttaccatcgt
     8161 taattcacta ggcttagtga atactcgctt ggcagtgatt ttaccagcga tggccagcgc
     8221 gtttaatatc tatctgttta gaaatttctt tatcgcgatt ccagaagaaa tcattgaatc
     8281 agcgaaatta gatggtgcaa atattgttaa aattttcttt cacatcatgc tgccaatgtc
     8341 taaaccagcc atagccacag taggtgtctt atcctttatc tccagctgga atgattatat
     8401 ttggccgtta atggtcttaa ctgacagcag caaattttca atgcaagtgg caattactac
     8461 gatcaatact acacagccag tttatatcaa tcaagtgatg gctgttttaa ccattgcaac
     8521 gattccactg attattgttt atatcgttgc acaaaaatat attctgcagg gacttggcgg
     8581 ttctggaacg ggtataaaat aggagtgaat agtgaatgtt gcaaaaactt ttaaaggcca
     8641 atgagtttat tgagaaaaat gctcaaacag tcaatcaaga ataccggaat aaatttcatt
     8701 tgatggctcc gattggctgg atgaatgatc caaatggttt tgtttattat caagaggagt
     8761 atcacttgtt ttatcaatat tatccttacg acagcatctg gggaccaatg cattggggcc
     8821 atgctaaaag caaggatcta attcactggg agcatttgcc agtagcactg gcaccaggtg
     8881 aaagctatga caaggaaggc tgtttttccg gcagtgccat agaaaaggat ggcaagctgt
     8941 atttgattta tacaggacac aatgttgtgg aagggcaagt tcgacaagtt cagtgtttgg
     9001 cagtttcaga agatggcatt cactttgaaa aatatgccgg aaatccaatc atcgcagaag
     9061 atcacttagc ttctgttgca acgaccgaag atttccgcga tcccaaagtg tttcagcgaa
     9121 aagaagacta ttttactgta gtcgctacta aaactgcaga tgctcgtgga caaattgtga
     9181 tgttccattc caaagattta ctaacttggg agttttattc cgtattgctg gagggaactc
     9241 cagaacaagg cattgtatgg gaatgcccag acttgtttga actagatggc aaagaagtct
     9301 tgattctttc tcctattcaa atgactccac aaggcaatgc ttaccaaaat acgaactcaa
     9361 ctgttgcttt catcggtact gtcgattggc aaacaggcaa atttctagta gagaattttc
     9421 atgaaatcga tggcggttta gatttttatg caccccagac ctgtgaaaac gaagcaggcc
     9481 aaagaataat gattgcttgg atgcaaatgt ggcagcgtaa aatacctacg aatgatctgg
     9541 gccatggttg gtcaggttcc atgaccttgc ctagagagct gagggtggct gataatcaat
     9601 tgttgcaacg gccagtttca tcaatttatc aagtaattga tccgcaggaa aggattgaat
     9661 gggcgaagac tgaagagaat cgcttcttac tgaaggactt agtatatgac cagcactatt
     9721 tacgcttgga aatcaattta aaacaggcaa cgaaactaga acttcactat gcgaagggac
     9781 ccgaccatac attactgctt aactatgatg ttgaacggga gtgctttact gcttccaggg
     9841 aacacatgcc gtatgattta tcaggagctg aaaaggaagt gttgaaaaag cgcagtgtga
     9901 cgattccttt ggaaaataat tgtctaacca tcgaaatttt ccgtgatact tcagctattg
     9961 agctttttct aaatgatcgt ctgactatga gtacaacttt ttatgagaca gaaaaaggac
    10021 aggattttct tttgctggca gacagtgaag taaccttttc attggaaagc ggcttaatta
    10081 atctatagaa gggagagctc agtgatgatt tatggttcaa tcgaagcagg cggcacaaaa
    10141 tttgtgtgtg ccattagcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
    10201 acgacacctg aggaaacaat acctttagtc attgacttct tcaaacaata ccaagcagat
    10261 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
    10321 tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DAPBCS010000077         8946 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPBCS010000077 DAPBCS010000000
VERSION     DAPBCS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.080"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(15..1154)
                     /locus_tag="RD521_002610"
     CDS             complement(15..1154)
                     /locus_tag="RD521_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDT7772277.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="RD521_002611"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="RD521_002611"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDT7772278.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /locus_tag="RD521_002612"
     CDS             complement(2967..3722)
                     /locus_tag="RD521_002612"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDT7772279.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /locus_tag="RD521_002613"
     CDS             complement(3826..5016)
                     /locus_tag="RD521_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDT7772280.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="RD521_002614"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="RD521_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDT7772281.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="RD521_002615"
     CDS             complement(6168..7259)
                     /locus_tag="RD521_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDT7772282.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="RD521_002616"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="RD521_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDT7772283.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="RD521_002617"
     tRNA            8802..8873
                     /locus_tag="RD521_002617"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2320 a         1897 c         1648 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagaa
//
LOCUS       DAPBCS010000078         8453 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPBCS010000078 DAPBCS010000000
VERSION     DAPBCS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.081"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1329)
                     /locus_tag="RD521_002618"
     CDS             complement(<1..1329)
                     /locus_tag="RD521_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDT7772284.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIKPVVLVNFNILKNISKFL"
     gene            complement(1470..2072)
                     /locus_tag="RD521_002619"
     CDS             complement(1470..2072)
                     /locus_tag="RD521_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDT7772285.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            2317..2523
                     /locus_tag="RD521_002620"
     CDS             2317..2523
                     /locus_tag="RD521_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772286.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            2835..3554
                     /locus_tag="RD521_002621"
     CDS             2835..3554
                     /locus_tag="RD521_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDT7772287.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(3635..4318)
                     /locus_tag="RD521_002622"
     CDS             complement(3635..4318)
                     /locus_tag="RD521_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDT7772288.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            4536..6722
                     /locus_tag="RD521_002623"
     CDS             4536..6722
                     /locus_tag="RD521_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDT7772289.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7068..7478
                     /gene="spx"
                     /locus_tag="RD521_002624"
     CDS             7068..7478
                     /gene="spx"
                     /locus_tag="RD521_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDT7772290.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            7492..7848
                     /locus_tag="RD521_002625"
     CDS             7492..7848
                     /locus_tag="RD521_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDT7772291.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3107 a         1382 c         1582 g         2382 t
ORIGIN      
        1 aaggaacttt gagatattct ttaggatatt aaaattaact agcaccacgg gttttatttg
       61 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
      121 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
      181 ttttgttgag aacccttttt gtttcaactg gtttaacggt aatgtatttg ttgttgtatt
      241 gatcacacga atcatcacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
      301 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
      361 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgatcaa
      421 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
      481 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
      541 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
      601 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
      661 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
      721 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
      781 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
      841 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
      901 gcggtccaca gtgtgaaacc aagtctctaa cgattttttt aaacaaaaag ctaataaagc
      961 agataaaaaa gcggataaga caatgtgaaa atactgcaat gaatctcctg gttcaataac
     1021 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
     1081 caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
     1141 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
     1201 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
     1261 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
     1321 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttggcgtatt
     1381 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
     1441 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
     1501 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
     1561 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata acgtaatgag agaaaaaatt
     1621 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
     1681 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
     1741 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
     1801 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
     1861 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
     1921 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
     1981 acaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
     2041 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
     2101 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
     2161 tcatgatctc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
     2221 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
     2281 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
     2341 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
     2401 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
     2461 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
     2521 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
     2581 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
     2641 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
     2701 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcttttaag
     2761 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
     2821 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
     2881 aaagcacact aactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
     2941 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
     3001 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
     3061 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
     3121 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
     3181 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
     3241 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
     3301 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
     3361 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
     3421 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
     3481 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
     3541 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
     3601 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
     3661 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
     3721 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
     3781 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
     3841 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
     3901 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
     3961 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
     4021 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
     4081 aataatcaat tagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
     4141 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
     4201 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
     4261 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
     4321 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
     4381 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
     4441 ctgttagtat aaagaaaaaa tagacaacga aactttaaat aaccaacttg aaataaacaa
     4501 attcaaagaa gaaagatggg aaggaaatca atcagttgaa tgaaaaagaa attctccgat
     4561 tacaaaaaga attgaaacgc tgggagaaca agtataaaaa gcttcaacaa gtctttcggc
     4621 tacaagaaga aatcattgct acctatgaag cggcttatgg cgaagcaccc ggacttattg
     4681 aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc tttctttaaa atgatggaga
     4741 aacgttccta taatcgcttc aatcaagaac aaaaagcagc tatcacatat gatatgaaga
     4801 aacatcttcg aatcatagca ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag
     4861 ctgtttatct gatgacgcaa aaacaagtag acgaagaacg catactaatg atcactttta
     4921 caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa tttcagccag agaaaaaccg
     4981 ctgtccatat cggaacattc catagtatct tttttaggat atacaatgaa atttgtcgaa
     5041 aatttcctga agtagcaatg caaggaatac aaggagactt ttcaaaggat actgcacaaa
     5101 aagtgaatgc tgttttgcaa cagctgatac gtaaatataa tctttatata tttgatcaat
     5161 atggagaaaa aacgattgct tctcggttag actattggca gaatatgaac ctttcagtag
     5221 aagaaatggt tcagttagtt aaagaaaaat atgattccat tgataagaat gcacgacagc
     5281 cgatatcaga acgactgtat ggattgctca cggaactcca agaacaaaaa agaagccagc
     5341 aattgctaga gttcaatgat gtactccaaa atctgaaaag cgctttggag aatgaggaga
     5401 tccagcatta tatcggccaa aaatatgatt atctattcat tgatgaattt caagatacta
     5461 atccacttca atgggaaatc gtgaaacgga tgacaaagga aaacaggatc aagttgattg
     5521 ttgttggaga tgatgatcag agtatctatg gattcagagg atcagagcca gcatacatca
     5581 agaactttga aaaagaatat cctaccaaaa ctcttttctt attaactaat tatcgttctc
     5641 gggcagctat tgtccaagga gcaaatagat tgatcagcta taataaaaat gatcggatac
     5701 caaaggcgat gattccagca caaaaagaag caggtgttat ggaagcctat ttgttttctg
     5761 atactaaagc agaaagtcag tggctaatca aacaaattca gacatttatt caaaaaaatg
     5821 ggaaatataa ggaatcaatc atattatacc gatcagcttc acaaacgcag cagcttgtcc
     5881 aatctctgct gaaaacgaat attccttttg tactggaagc agatagtcca tatgaaggta
     5941 tctttggtat caagtctttc caacatttct atcaaaaaat tgttcattgg caaacagcac
     6001 tgaatctgca aaagaaaaat caggcctacc agcaattgct ccggcagctg atggcagact
     6061 gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc taaatatcaa aatatagccg
     6121 ttgcagatta tattttgtcg ataagcccca atttaaaatc gaaatctgca gaaatccgag
     6181 agtttgagga agcgttgcgt aaaacagacc agcatcaaga aatctattcg cttgttcaaa
     6241 gctatcttca tctgccacgt atagctaagg aaatcgacca gagtgatcaa gaatggatac
     6301 gagaagaagt aaagcagcat gctactttca gaagactcca agccttatcg caagaaaccc
     6361 aagcgacagc taaagaatta aaaacccgct tgattgctta cagacaagga aaacttgatg
     6421 cactatgtat ccagagcatt cacaaaagta aaggtcttag ttaccgaaat gtctttttga
     6481 ttggttgtaa cgaaggaagt ttgccttata acggagcgac agaaaagaaa gtaatcgact
     6541 ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga agaacgaaga ctattttatg
     6601 ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa
     6661 gaaaattgaa agtatcgaga tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt
     6721 gatagaagag agggtgtgac agaagtggtc agtttcaaga aagaagaagg aatgtacgaa
     6781 aattgcttct caaatttttg tgaaatttca gcttattttc gaagaagcta tttctggaac
     6841 acagtttatt cggaaaaggg aggtgtgaca agaattttgt cacacctccc ttatttattt
     6901 tgaaaatgag aatactatta tcaaaataga gaatatatag aaaaattctc attattcaaa
     6961 cgtaaataat cagttatttt gatttttttt atattttatt agctaataat tggtatttta
     7021 aaagaaatta ctgtagaatt ggattataat taaaaaaggg tgagaaaatg ataaaagtat
     7081 atacttcgcc tagctgtact tcctgtagaa aagcaagagc atggttgcaa gcgaatcaac
     7141 ttgaatttga agagaagaat atttttgccg aaccgttaac agaaagtgag atcaaaaaga
     7201 tcttggcagc gactgaagga ggagttcaag atattatttc cactcggtcg aaaatttacg
     7261 aaaagctggg catagatttt aacgaattaa ctttaaagca aatgatcgtc ttattcaaag
     7321 aatatccttc attattgaga cgacctattt tgattgatga aaaaagaata ctaatcggat
     7381 ttaatgaaga tgagatccgg tcatttgtcc ctcgtgaaat tcgatgtgtt gccagaaatc
     7441 tgatctattt acaaaacaat aacatcttaa attcataggg agggagcctt cgtggaagtg
     7501 aaagtctatg gatcagcgca ctgtgtaaca agtctgaagg cgaaacaatg gttagaacac
     7561 catcaattgc cttatcaatt tgttgactta gaagaacgcg atctaagtac agaagaagca
     7621 gaagaattat gcagttttga agaagtgcaa gcagaacagc tatttgcgac ttggtctgag
     7681 gcttttcaca acattgcgat tgacctttcc tgtgtaggaa aaaaacaact gattttttta
     7741 tgcacacaac ataccaatct cctcagacgt cctttgatta ttatcgatga agttctattt
     7801 attggataca atgagcagtt gatggaacag cttatcttaa ataaatagac tttaactgtt
     7861 ttaatcaagt cactgagcat ttcatacagt tgtaacagtc gaacacttag catcaagcgt
     7921 gttatgtgtt ctttttttga ctttattttt ctactataga gacatgctca atttatgtgg
     7981 tcaaaattca aatctaggtg gggatataga gttaaaaata tattgacatg tgtaaattta
     8041 catgatatat tcatttctgt tgttttaagt agcaaagcaa taaaatagta ttttaaaaaa
     8101 catataaaat agttgttgac atgttagaaa tgacatgata agataactga gttgttaaaa
     8161 aagaaccact tgtgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
     8221 aacaaatgat aagttatgat aaatgagttg ctgtaagatt tcgaaaaacg aaatcaaaaa
     8281 aacttttaaa aaagagttga caattacttg tctctctgat atgatataaa agttgctacg
     8341 gcagccaaaa acgaatcggg gaacactcct gatgaagtag acctttgaaa actgaacaaa
     8401 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa aca
//
LOCUS       DAPBCS010000079         8323 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPBCS010000079 DAPBCS010000000
VERSION     DAPBCS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.082"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..278)
                     /locus_tag="RD521_002626"
     CDS             complement(<1..278)
                     /locus_tag="RD521_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772292.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFA"
     gene            complement(329..1957)
                     /locus_tag="RD521_002627"
     CDS             complement(329..1957)
                     /locus_tag="RD521_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772293.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            complement(1977..2144)
                     /locus_tag="RD521_002628"
     CDS             complement(1977..2144)
                     /locus_tag="RD521_002628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772294.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(2181..2870)
                     /locus_tag="RD521_002629"
     CDS             complement(2181..2870)
                     /locus_tag="RD521_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDT7772295.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(3125..3445)
                     /locus_tag="RD521_002630"
     CDS             complement(3125..3445)
                     /locus_tag="RD521_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772296.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(3478..4596)
                     /locus_tag="RD521_002631"
     CDS             complement(3478..4596)
                     /locus_tag="RD521_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772297.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(4625..5419)
                     /locus_tag="RD521_002632"
     CDS             complement(4625..5419)
                     /locus_tag="RD521_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772298.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(5429..6304)
                     /locus_tag="RD521_002633"
     CDS             complement(5429..6304)
                     /locus_tag="RD521_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772299.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(6521..6778)
                     /locus_tag="RD521_002634"
     CDS             complement(6521..6778)
                     /locus_tag="RD521_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772300.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6781..7005)
                     /locus_tag="RD521_002635"
     CDS             complement(6781..7005)
                     /locus_tag="RD521_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772301.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(7178..8221)
                     /locus_tag="RD521_002636"
     CDS             complement(7178..8221)
                     /locus_tag="RD521_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDT7772302.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2354 a         1702 c         1033 g         3234 t
ORIGIN      
        1 caagcaaaaa tttctaatat cacattaaat cttttcattt ttccaaaact actaagactt
       61 gatactaatt caatcaaaag cttatcgcta cttgctgatt catcatattt agttcctaga
      121 ttatctaaac ttagcatata agaaattttt tcctgatctg ttagttttga aaatgaactt
      181 tcttttaaac tttggattat acaatcagca ctaaatattt cgtttaataa agcagttttc
      241 ttttctaatt ccttagaaga taaagttagt tctctcatag tgacttacct actttccaaa
      301 ttttgtgtcc tatttatcac ttctctcttt atataagtga taaaagatcg taaatacgtt
      361 taattgttaa gttcatattc acatgattat ccaaataaaa tatcttatct ttattagaaa
      421 tttcactcat aacacgataa tatagaccat gattcaagta atctgtatat agaaaaatcg
      481 catcacacgt attcaaattt ttaagtatct ttccattatt ttcgcttaca tcaaacattt
      541 taatatttgg taatagttca gttaatttat tttgaaaatt tacatgtcct cccactaaaa
      601 gaatgttttt atgttttaga taagctatca tttcagacag tgaaggctct tcagttttac
      661 catttaatga ttgtctatgt tcttgctcct gcaattgctt taatacttta ttttcaaacc
      721 tcagctgttc aagttctttt ctgattcctt ttaattcttt aatctcttgc ttcaaaagtt
      781 cctcttcttt ttttgccgat ttgactaaag attctaaccg accgttttct ttactcgcaa
      841 tacttaattc ttttcttaat ttgattactt cagatttgtt ttcttttcta atacttgcaa
      901 tttgttttgc tatttcataa tcagaattca ggatcaattc ttccttagca tttttaaaca
      961 accgagttaa aacgaactga tataacaaac tataaaaaac tagcaaatag tcaatttgtc
     1021 ggtactcggt attctgatca attgcatata gagagattaa aacgaaatac acaatatcat
     1081 tcagctcgct acccttgatc tcaaaattat tgactaagta actatcaata cccatcaaat
     1141 ctgcaaaatc tccatacaaa gaaaaatatg attttgacaa tatatctata tctgaatcgt
     1201 caaacccatt tactattttc ccatcattgt tagtagttgc taacgaagca atagtgactt
     1261 ttgaattggg ttttacaaat cctataccta atagttcttt catcttttta atttcaagtt
     1321 tgttatcctt ttgttcggct ttatgctgtt tcatattgta aagcggataa aatgctgttc
     1381 cgtaagcacc ttttagtacg ttatctataa aataatcttc agaacctgtt ttgaatagaa
     1441 agttaatcgc agccaaatta gataacagct cttcagtgtt gtgcatcaat tttttatcat
     1501 ttaataaatt atccccaaga tatttcattg cataataagg gtttgtttcg ctacttattt
     1561 ttaatatttt ttgatagcta ttcctcacga tattaaccaa aatatctgtc tgattaattg
     1621 aattacagta caaagccact cctaatgcca tataaacatt caaatcatgt tttgccgtac
     1681 cgttgaataa aacgtcttca acttttgaat tatctcgtct atactttttg atcatatttt
     1741 tgatattttg atcattctct tggtatattt ttttcactcg ttccaatgcc ttgggtttgt
     1801 tggaaacagc aacagcgaag tctagttctt tgaaatcaat atttagtatc tgtacctcat
     1861 cacctagatt ataatctcta tttacgctcg gaaaactgct gaaatctaac tgttttttac
     1921 ctatttttgg cgaaaatatt ccttcgattt ctggcaaatg taatcacact ctctccttat
     1981 ttatctttag aaaatttgtc actattctcc ttatatttat caataaacct ttttaatcga
     2041 gcaatataag cgttaaccct tttaggttct tcttttcgcc attcatcgtt tacgtgacca
     2101 ttttcaaaga aagaagctaa aagagtttcc acttcattaa ccatatctgc gatcgtatat
     2161 ttttctgtac cctcactcaa ttattcatga actgttaagt gttcatagct aagttcattc
     2221 ataattttgt tatagcgttt tcttgctttt tcaaattctt tctgttcgtc aaattccact
     2281 tgattcttca tgttgttctt cctctctgtt tgatattttt tcacttcttc aagtttagca
     2341 tctagttccg ctttaacttc tgtcacttct ttaagcgctt gggataactt atgagacaat
     2401 ttcgcataag tttctctctt gtattcattt cctactccca actcttcgga gtagaactct
     2461 tctagtagtc ttgcaaaatt aacatttctc ctgccagcat agttttttag cttctcaaaa
     2521 tcagattgaa agacagcaac acttttgtaa tccttttcac cctcaaaatc aaaagcatag
     2581 tcatactttc ctgaaatcaa ttcatcaata actgtagcaa tatattgaga gatagaaata
     2641 ttatcttcac ttctatgtcg ctctataaga ttatatatat cagggctgac atgaaacgta
     2701 cttctctttt ttgtttgcct ttttctaact gttacgacca cttttggaac ttcactatct
     2761 ccattgatct ctttgtattt ttcaataaat aattttctag cttgttctcg actttttgcc
     2821 ggaatttcat ctacaatttt atcttctaaa taaatatagt gtgttctcat acattcgtcc
     2881 tctatcttct ttactctcaa tcttcatgat cttagttctt accttgttta ctcttaaaca
     2941 ttgcaaaaaa atctagtcaa aatcaaactt gaaatcatcg tctatcacaa cacccttaac
     3001 ttcatcttta gatttcccaa aggaaccctt attttcaaat cctttatttt tgaatgatga
     3061 tggttatgga aaacttcctt tgaaattctg acaagcataa ctagtcagaa ctgttttact
     3121 tagattaagc tttcaaaaga tactgatgaa gaactaagtt ctcagtacct tcccattctt
     3181 caataaaaaa tatttctcct tcttttaccc actctatagc taatttctta taatcaaata
     3241 aaccatatgt gtttaagcct ttgtctttca ttaaactcaa aaactgatcc ccttttaatc
     3301 tttctgtatc atccatcaga aattcaacta aatctttatc catgcgtgca actttttcgt
     3361 cttttagtgc cattgattta gcatagtcag ttgaatcttt tagaataata acacctacat
     3421 ttccttccac aattttcttc ttcatattta cctcctacca tcttttccaa agagtattta
     3481 gtcaaaatca aactcgaaat catcgtctat cacaacaccc ttaacttcat ctttagattt
     3541 cccaaaggag cccttatttt caaatccttt gtttttaaat tctaaagctt tttcgacttc
     3601 ttctttggat attgtatgaa cttttttctt aactactcca agtttataca gctcttcaaa
     3661 ctctggtact tcggatctga aatacaatcc atcagtacga atttttaata gcccatatgc
     3721 gctatgaacg tatttctgtt catatctaac catacgatct ctaaatacgc ctgctaccat
     3781 ggaggaatac gctcgattat acatactgta tttatcgaag gtgcttgttt ctccttctct
     3841 taatgatttt tgtccgatag caccaaatac tttcgttcta agatttttat aaagcttcaa
     3901 cgtatcagga tcagttttta atggctcgat tctttcaaaa tattctttga gtaatccttt
     3961 tttcgtcaaa aatgtgtggc tatctaggac ttccatctct ttaatatcat aataatcaag
     4021 gataagtgtt agctcaatct ccgataacca aataggctca ttagctgttt tgggattttc
     4081 gccataatcc gcaaacatcg actgtgattt tggattagca tatgttcctt ctttggctgt
     4141 tccttcaaaa ttaaccaaaa agaacatata cgtttttaaa ttttcatacg gtcttacttt
     4201 tggtctattt atcgctctca aaatagcttg atctgtatta tattttgctt tcaaataagt
     4261 gtttgacggc aaaggataat ttctcatgat cctcgaatag gcttcgttga aatcatattc
     4321 gatcatcttt gctccttcct caaacaaatc caagttatac atgctaccgg aggtactggc
     4381 tctataggct gaacttggaa caaagaaagg tgcatcttcc ggcttatcat agcccaaaaa
     4441 atgtattcct gtttgcttgt tctgacggta aatgtccatc aaaagatatt tgatcgtact
     4501 aaaagaatct aattttcctc ttgtcatcca ccctaatccg acaagtcttt taattttatt
     4561 ctgcatttca ggtgtgtata tgctattgtt tcccatttat ctgctcatct ccggcttttt
     4621 tattctagac cctatatttg ttcattacac cacttatctc gtctacaaag gctcccagat
     4681 cgtgtgtaaa ggcatcatag taatcttcaa tctcactcga ttcagttctc cggtttcttc
     4741 cgtttttatt tctgaataga tacatctgcg tttctcttaa atcccttgct tttttcgcaa
     4801 gtgctaataa atccgtaaaa ttatcaataa ccataatgtc attgatcata aaaccttctt
     4861 catgaccata cttttcagcc atctcgataa aatcattaac atcgaacaac tccaataatt
     4921 ctgctttata tttctctaac aatgtattta aatagcgatt tctcaattcc catgaacttt
     4981 caccagttcc gtattcaacc ttgatcccta aattcttagt gtagcctttt gctgtgtatc
     5041 gtccaatgaa gtctccctta ttccgattgc tatcttcttc tttgaaaaac tcacgccaaa
     5101 tccccttttt cttcttagga attagacctc tgctcttgaa atcatcatat ttttttgaag
     5161 cttctcttcg tgtatctaag tacctttgct tcagctctgt agctgattca ttcgttccat
     5221 attcaacagt gatccttaag cgcctgctat aaccttttgc tgtatatcgt ccaacaaagt
     5281 caccgccact tcgatcacta ctgacttcct taacaaattc tctccatata ccttttttgt
     5341 cctttggtat taatcctcgt cttttcatat cttgatattt ttcagaagct tcttttagag
     5401 tattcttttt tgctaccata ttttccactc actctttgtt atctagtatt ttaataactg
     5461 gtttcatttt tgtaatatct tctttttcag ctatatacaa ctccgttacg gtaacgatct
     5521 tagtatgatt tagattaatt actttcactt gatcgccaat tttgattttt cctcgcaacc
     5581 gtttgggaat aaaccagttg tataatcgtg atttattatt atctacataa gcttttattg
     5641 cattttcagg aaacaggtta tttagtcttt caattttttt acttttaaaa aattcattcg
     5701 caaacttttc atcaacaact aaattaaata aagtttcaga atttacagct tccatatcta
     5761 ttcctttgta gataggagaa agcaattttt tatattttgc cagtaatgac tttccagaat
     5821 tactaatctt aatttggtta cttaaatctt cttgataaag aaccccatat cgaagtaacg
     5881 ccttaatata atgttttaat atgccgttga taccagaata aatatccatg ccgttctcaa
     5941 tatagtagaa agtgtcgtct ctccaaaaca atttgaagct attattcaaa taaagtaaca
     6001 agatcaatat atgtttgtca gaaattctga caaatctatt actttttaga tactcaccaa
     6061 tggcttcact taactctttg aagccattgt attttcccga taaaaatgtc gtaacattat
     6121 cttctctctt tttcctatgg tgtgaactta ccctgaaata atagagtcta ttctcattta
     6181 atattgaaaa ataaatactt tcagttgttt gtgagcattg tatatagttg aaatgatatc
     6241 catctggcat gactttttta actgtatttt gtactagctc tgaccacctt ttgttagtag
     6301 tcatatattt ttttccatta aaccaaacat aaatatcttt tttaaaaatt ttgttccccc
     6361 cctattgcgt tttctttgac attaaagccc tttgttttca tcacattcgt atagccctag
     6421 ccactcataa cctgatgctt ttgcgaagtt tatttaaaag tgttttcctt tgtaatctac
     6481 ctttcccctc gtcctggtac ttttcttggc gactattaaa ctattcaact tttttagaat
     6541 cattgtcagc ttgaaatcct tcacgtttca ttatgtcttc tgcttttcca atgctgattc
     6601 ctttccaatc acgccatcct acgcttccct tattttgacg ataggcttta atagctgatt
     6661 cggatatttg ccatcttgta gccaactctt tggctgtgta cttatttgaa aaaagtagct
     6721 tctctatcag tgaaatatca atcattttat tctcactcct gaaatcttca ttaatcatct
     6781 ctagctagct atatcttcaa tcaattctaa tatgtcggat aaaccatcaa cagcttcttc
     6841 aagttgatca acggattctt ctgctttttc atatctttct gatccctgta gatttttagg
     6901 tatattttct ttgtactctt cttcttgaat tacttctaac tcatccttta gctcggaaag
     6961 tcgctcgctt atttcttcta acctttttct tcttctttgg ttcatcaaaa tcacctcttc
     7021 gcataatctc tacgatttta atataccact tttgtgtact acattcaata ttttttgtta
     7081 tagattcgta ttacaaaaat ttcctctaaa aaaataagag cctatttacc atttcagtaa
     7141 ttaagctctt atttcattta gttgataatt ctatcaatta acgtacagca accgccattc
     7201 cagtcacatt aaatgctctc tcatacgtgc ttgcattgat ccattttgcg ccaaatacag
     7261 ggtcaacaac atggtatgca ccatttctga agccatccac gagaactaca tgtccattcc
     7321 atactgcacg accccaaggc atttgtttga actctggcgt agcatagtta taagtaatcc
     7381 aagttacaac cggattacca ttgcgaattt cattcttgat tccattcgtt cctgaaccag
     7441 tgatattgac ggcattaccg ccaacgtttt ttgcccattt aactactggg tcagccatca
     7501 tgccttgata cgttccatta acgttatgac gccactctcc tgagtaacca tgataaggat
     7561 tattgtcatt agcttttggc atatagttta caaaactggt taagctttga tttgtggcta
     7621 cacctttgta gtgtaaacct tctaataaag aagctccttc gcataacatt gtatttcctt
     7681 gccatacaac aggaacgttc aacacttttt cagtgcttgg agcaggtgta ggatttggta
     7741 cgctgtttgg cattgcatac cagcctgtac cttcaatctt ccaaccttgt ttactaatta
     7801 cgttcttttc attttgatct aacgtgtagt gatgccaatt cacgtatttg ttatataaac
     7861 gtgttacagg aactctattt gctccgttgt aggaatagaa cgcaacacct tcgtacgtcc
     7921 atcctgtgtg tgaaatctta tctctttctc cggcatcaac tgtgtaaaaa tgttcaccgt
     7981 tataccgatt atacaaacga taaacaggag taccacttgt tggagcttgc catgcgacac
     8041 cttcgtaatg ccatccataa cgtttcagat tgtctctttc tccaacgctt ctagtgtata
     8101 gatgctctcc gctattggga ttatacatac gatacatgga aatagagctt tctgctgaaa
     8161 ctgaaggtga taataatact agccccattg tgaatagaat tcctactaaa aattttttca
     8221 tttgatttta ccccttttca ttttttattg tcattattgc atgaaaatta ttcgtaagat
     8281 tgccaattac ataaacgatt gttttaagat cataatcgtt gtt
//
LOCUS       DAPBCS010000080         8251 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPBCS010000080 DAPBCS010000000
VERSION     DAPBCS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.083"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..197
                     /locus_tag="RD521_002637"
     CDS             <1..197
                     /locus_tag="RD521_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772303.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="RD521_002638"
     CDS             314..811
                     /locus_tag="RD521_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDT7772304.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="RD521_002639"
     CDS             898..1290
                     /locus_tag="RD521_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7772305.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="RD521_002640"
     CDS             1274..3721
                     /locus_tag="RD521_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDT7772306.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="RD521_002641"
     CDS             3724..5907
                     /locus_tag="RD521_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDT7772307.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="RD521_002642"
     CDS             5904..6905
                     /locus_tag="RD521_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDT7772308.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="RD521_002643"
     CDS             6920..7834
                     /locus_tag="RD521_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDT7772309.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="RD521_002644"
     CDS             8079..8195
                     /locus_tag="RD521_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDT7772310.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DAPBCS010000081         8228 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPBCS010000081 DAPBCS010000000
VERSION     DAPBCS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.084"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="RD521_002645"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="RD521_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDT7772311.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="RD521_002646"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="RD521_002646"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDT7772312.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="RD521_002647"
     CDS             2591..3376
                     /locus_tag="RD521_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7772313.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="RD521_002648"
     CDS             3389..4240
                     /locus_tag="RD521_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDT7772314.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="RD521_002649"
     CDS             complement(4364..4816)
                     /locus_tag="RD521_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772315.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="RD521_002650"
     CDS             complement(5281..5649)
                     /locus_tag="RD521_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDT7772316.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="RD521_002651"
     CDS             5799..6281
                     /locus_tag="RD521_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDT7772317.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="RD521_002652"
     CDS             6318..7328
                     /locus_tag="RD521_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDT7772318.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="RD521_002653"
     CDS             complement(7443..8156)
                     /locus_tag="RD521_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772319.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DAPBCS010000082         7598 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPBCS010000082 DAPBCS010000000
VERSION     DAPBCS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.085"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(941..1354)
                     /locus_tag="RD521_002654"
     CDS             complement(941..1354)
                     /locus_tag="RD521_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDT7772320.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="RD521_002655"
     CDS             complement(1810..2295)
                     /locus_tag="RD521_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772321.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="RD521_002656"
     CDS             complement(2434..2649)
                     /locus_tag="RD521_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDT7772322.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="RD521_002657"
     CDS             2873..3673
                     /locus_tag="RD521_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7772323.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="RD521_002658"
     CDS             3692..5560
                     /locus_tag="RD521_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDT7772324.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="RD521_002659"
     CDS             5553..6044
                     /locus_tag="RD521_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDT7772325.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="RD521_002660"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="RD521_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDT7772326.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="RD521_002661"
     CDS             6604..>7598
                     /locus_tag="RD521_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDT7772327.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DAPBCS010000083         7314 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPBCS010000083 DAPBCS010000000
VERSION     DAPBCS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.086"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..226
                     /locus_tag="RD521_002662"
                     /pseudo
     CDS             1..226
                     /locus_tag="RD521_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            242..391
                     /locus_tag="RD521_002663"
     CDS             242..391
                     /locus_tag="RD521_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772328.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            472..1068
                     /locus_tag="RD521_002664"
     CDS             472..1068
                     /locus_tag="RD521_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDT7772329.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1081..1980
                     /locus_tag="RD521_002665"
     CDS             1081..1980
                     /locus_tag="RD521_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDT7772330.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1983..2114
                     /gene="ssb"
                     /locus_tag="RD521_002666"
     CDS             1983..2114
                     /gene="ssb"
                     /locus_tag="RD521_002666"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7772331.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2136..2468
                     /gene="ssb"
                     /locus_tag="RD521_002667"
     CDS             2136..2468
                     /gene="ssb"
                     /locus_tag="RD521_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7772332.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2490..2747
                     /locus_tag="RD521_002668"
     CDS             2490..2747
                     /locus_tag="RD521_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772333.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2906..3028)
                     /locus_tag="RD521_002669"
     CDS             complement(2906..3028)
                     /locus_tag="RD521_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDT7772334.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3218..3442
                     /locus_tag="RD521_002670"
     CDS             3218..3442
                     /locus_tag="RD521_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772335.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            3890..4096
                     /locus_tag="RD521_002671"
     CDS             3890..4096
                     /locus_tag="RD521_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDT7772336.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4096..4347
                     /locus_tag="RD521_002672"
     CDS             4096..4347
                     /locus_tag="RD521_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772337.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4363..4800
                     /locus_tag="RD521_002673"
     CDS             4363..4800
                     /locus_tag="RD521_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772338.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            4793..5488
                     /locus_tag="RD521_002674"
     CDS             4793..5488
                     /locus_tag="RD521_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772339.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            5869..6048
                     /locus_tag="RD521_002675"
                     /pseudo
     CDS             5869..6048
                     /locus_tag="RD521_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6110..6294
                     /locus_tag="RD521_002676"
                     /pseudo
     CDS             6110..6294
                     /locus_tag="RD521_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6328..6759
                     /locus_tag="RD521_002677"
     CDS             6328..6759
                     /locus_tag="RD521_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7772340.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            complement(6933..>7314)
                     /locus_tag="RD521_002678"
     CDS             complement(6933..>7314)
                     /locus_tag="RD521_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7772341.1"
                     /translation="KPEGRKVVDIENSINEWLQSVPKNLFKSITFDCGKEFSSWKSIS
                     NTNDIDIYFSDPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGFISSVASKRNHIP
                     RKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         2588 a         1219 c         1485 g         2022 t
ORIGIN      
        1 caagaagccg cgcgtcattt aggaatttgt ccaaaatgcc aggaacaaga agtcttactc
       61 tatcaaaagg tggcagcttg tacgtctgaa gcctgtgatt ttaaaaggtc agaagggttc
      121 atttgaagca acgattgttt taaaagaaga cttcactaca ggctttgaat tttctgaaaa
      181 gaaaaaaacg aacttcaaga aaagaacacg ccgcactgca aaataatgga gggttaatct
      241 tatgcgtaat accacacaca aacaacaaga aatcgaaaat caaaaagcca aaattcgttc
      301 agaacaggaa aaaatgcgtg ccagcaaacg aagactgcag caattgaaga accagcagag
      361 gattcaaaaa gaaatcgaat tagatcgata aaatcttcct aactattgta tacgtataaa
      421 cttatgttat actgttttta taagtttata cgtatacaaa ggagggagaa aatgacaaaa
      481 gcaatggtat acaagctgct agaagaattt aatggaactt tggctttgaa ggatgccaaa
      541 aaagcgggca tttctcctgt aacgatcaaa cgtatggtag atcgagggga gctggatcga
      601 gagtatccag gttttttcac cttacctggt caatttcctg atgaattgtt tatggcgcaa
      661 aaaaaatatg aacgcggcat tatttcacat attaccgcgt tagatttgta tgatttaaca
      721 gatatgatcc ctagacagat cgatctgact gtaccttacg gttatcacgt ttctgaaaaa
      781 ggattgaagg aattcgcagt tgagctacac tatacaaaag cggaatggta tgaattaggt
      841 aagattgaaa taaaaagtcg ctatgggaat cctattatag cttatgatcc agaaagaact
      901 ttatgtgaca tttggaatcc ctggtatacc gttgaggatg aaatcaaagt aaaagcaatc
      961 aaaaactata tggagtcgga cagaaaaaat cttagaaaat tgaatgagta tcgacgaatt
     1021 ttaccaacag acaaaacgat gcgttcttat attatggctt taaattaaag gagaagatac
     1081 atgacaccta cgcagttaaa agcaaaagtg aagaatattt ctagagaaaa agaagttgat
     1141 ccgcagcttg ttttaagaca ttttatgatg gaaaaattct tggaaaaaat ctcagagtct
     1201 ccttatcgtg aaaattttgt gttaaaaggc ggcttcttga ttggctcaaa gtatggcatt
     1261 gaaaatcgca caaccaaaga tattgatacg actttacggg aaatgaaagt aaccaaagaa
     1321 acattaacaa cggttctaaa cgaaattttt tcaacgccga cgaaagaagg cattcaattt
     1381 gagattcaag gaatgaaaga aacgagagaa gccgattact atccaggatt tagcttacgt
     1441 gtgctggcac atttggaaaa catgcggcca gattttaagg tagatgtgac tacaggtgac
     1501 tcgatctatc ctgcaacgat cactcacagt cataaattga tgtttgagga tcgaacgatt
     1561 cctttggaat cctatcctac agaacaaatt attgctgaaa aattaagtgc aacctttgat
     1621 tttttaacag ataattcgag aggaaaagat ttttatgatc tctatactat tcctaaaatg
     1681 gagaagatcg atgagaagac cttgaaagat agtgtgagaa atacatttac acgtcgagga
     1741 aaaactgatc cgttaaagat atactatgaa aaggatatgg atgtactgaa aaatagtcac
     1801 gagctaaaag atacctggaa gaaatatcaa aaaagcaatc cttatgctgc gtcgatcacg
     1861 tacgatgaga caatggattc gattagtaat ttgatggaaa aagtgattca cgtcgaagaa
     1921 aaagagcgaa aactagctta tcaaagacac caacagatga atagagaaat ggagagataa
     1981 agatgattaa taatgtgaca ttagtaggac gcttaaccaa agatcctgat ttacgttata
     2041 ccgcaagtgg tactgcagta gcgactttta ccctagccgt caaccgaaat ttcacgaatc
     2101 aaaacggcaa ttgagaagcc gattttatta actgcgtgat ttggcgcaaa ccagcagaaa
     2161 caatggcgac attagctaaa aaaggtagct tgattggtgt agttgggcgg attcaaacgc
     2221 ggacttacga caaccagcaa ggacaacgtg tatatgtgac tgaagttgta gcagataact
     2281 ttcaactatt agaaagtaaa gcagcgacag agagccgtgc gcacgctgat caatcgtcca
     2341 ccagtccaag caccgccacc tttgaacaac ggaatacagc gacgcctaac aacaatggat
     2401 tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat gatgatctac
     2461 cgttttagtt tgagaaagaa ggagtaagca tggcaaaaaa aattagtctg acgatggatc
     2521 tattgatacc cgtaattgca tttatagacg ggatttcttt gggcgtcact gaaaatcctt
     2581 tggtaaatag aattgcgaaa ggatcactat ttcttgtatt cgtgtatctt gtctgttctt
     2641 atcttttaaa aagagcagga aaggataatt ccttttggcg attgactaaa gtcacagtag
     2701 cagcgacagc tgttatactt acggctattc taccgcttta tatgtagagt taacgggaaa
     2761 tcgttgaact tcttgcgaaa tattgctata atgaacatga aaaagaggag ttgcgctaac
     2821 aacccctcat taacagcacc gattaaagaa cggtggcaga atattagtta tttggaaata
     2881 accgctgacc tgctaaagta gacggttatt tctttttgtc atttttaagc atatttacaa
     2941 tcaaactaat caaagcaatg gtaaacatac caaaaccaag aatggtttga attgtctcat
     3001 atgcggacaa aaggcttact cctttcatag attttagcac ttacattcat aagcaccacc
     3061 acctttccag aagtcgccac cgtcttcaaa ctttactgtc ttgactatta tagcatatct
     3121 aaaatcgaat aaaaattctc aattttagct gtttttgcta taattttagc gaggtgatcg
     3181 tgatggaaca atatattggt ttactcgata aattagggtg atcaagacac agccattgct
     3241 tgtgcgattt acattacgca cgagtcatga accaatcaat tgtgtagtgg ccaatataga
     3301 aatagcggat aaattgctga ttttggaaga aggcaagtat actatttcag tgacaggtca
     3361 cttcaataaa cgaaatcaac tagtcattga atcaatgcaa attagaaacc ctgatcattt
     3421 tacgagatta atgggcatat aaataaaaga agagctgcag tcctaaatga ggattgcagc
     3481 tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc tttttaggaa ggcgcaagcg
     3541 ccttctcaat tgaaaaacct ctctctcact ccaaccgcca ccgccgttcg agaagaaagg
     3601 aagtgaacgt tgcacgttca ctaagataag gggtcttgca aagcaagaca aaatagaaaa
     3661 aacttcctct caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc
     3721 ggcaacaagt tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc
     3781 acgcccttgt ccttgtcgct tttagttgat tcattctggc caacaaatca ataaagaaag
     3841 aactaacgaa aggaagtaaa acctttccta agaggaggca gaatcaatca tgcgaaagaa
     3901 atttgattat tggggtgttc ccttcagcga attattccca aactatcatg caccatatac
     3961 tgtcgaatgc gattgtggag aaagagcgaa atgtatcaaa agctattgtt tgtatcaatg
     4021 tccaacatgc gggaaaaaat ataccttgtc ttatggcgat tatattttga ttgatgagaa
     4081 aggaaagaaa cgctgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa
     4141 attttggtgg acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg
     4201 tactggacga tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta
     4261 gtgaagattg caggatatga actgcattat caagaaaaca tggaaactta tgaaaacgaa
     4321 tattggttaa ccaaattaga gaactaaaaa ggagaaaaaa atatggctca aaattactat
     4381 gacgaatttg tcaaattacc tttggataaa atggcacaaa aaatggagga tatgacattc
     4441 ctatacaatg agacacgtgt tccaaaaaaa cattataagg aaagattgtc ggtagctgtg
     4501 gaagaaatga ttgaatcggg tgtcgaaatg aatctcattg cgacctacta tcgaacgctt
     4561 gaagaactga aaaagcaaaa tggacaatgg tttttccaag ccttactatg cttggaagca
     4621 ggcgtcaagc caaacactat caaaccttct gaataccaag ctttagaact aacgtatggg
     4681 aaatttgtcg aaacgaaaaa agccaagact gtttcaagtg aatggttgga ctacttagaa
     4741 aatgtcaaca agtatggcgc atgttacatc atgaaaaagg aggacaacga aaatgaataa
     4801 ggtgatttat caactaacaa cggaagatgt gtcacacttt ttaacaaaca aagaacattt
     4861 gaatgaagta atcagacgga ttcaaaaaga ctttagttgt atggaagaaa tgaagtactt
     4921 tattcaaaga gtcgaagaac aattgtattt ggaaagtgaa acgagtcaaa cgaaggttac
     4981 agtcagcaat aattatttag acgtgtataa acgtcgtcct gacacattaa aaaaaggagc
     5041 ggagcttcta cgactagaaa cagaaaaata tatcatgaat gtttacctag aaaggagtga
     5101 actggtcgaa aactttgtca gctacacgat ttgcaggaaa cactattact cagaatcaga
     5161 atattattca atttttgaga gcatggaatt aatggaagag ctaaacagca aagatgcgac
     5221 ggtagacgat tattaccttg tatttactaa atttttgacg tgtgggacgg aatctatcgt
     5281 gttaatggga atggattatg aactcgctgt acccgaagta tttttgaggg aatatgtaga
     5341 agaagtagat aacccaggcg acgactggta cacgacttta actgattttt tagataccta
     5401 cacttacgac gatacagaag aaatccaaaa tgaacttgac cgtaaacgaa tgccatatca
     5461 acgatttgaa aggaaaaaag gtcaataacc atttataccg aaaagtgaga atgtgtgaaa
     5521 taaaaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa tcgctccaac
     5581 cgcccccgcc gcttaggtgg tgcgaaataa gcgagcaaag cccgaaaacc taagagaaag
     5641 aaggagattt atatgaacaa aattattccc ttattattag tcctatttgt tggagaaata
     5701 cttatgcgac ttagtgcagc tgtacattta aacctcgcag tatttttggt aatactaatg
     5761 ttttgtttga tcttttggcg atttgtatta gccctcgtaa gtctactatt caagatgatt
     5821 gcagccatat ttttcatagg attatttcga tgaaaggaga gagtaccaat gccaattgca
     5881 ataggaaata aacggttacc cgtgacgttg gatgaaaaga gacaaaaaga attgcagtaa
     5941 ccaaagcaga agtacggcaa aagtgaatcc aggattatgt gtattgcgtt agatttattg
     6001 attgcgcaag aaaaagcagg atttgacata ccagcactca aaaagtgatc gcactttgcg
     6061 cacgtgttaa tacgtgttat aattattttg taaggaggta tagtgcacaa tgccacttac
     6121 gggaaaagaa atgcttaagt tattgaaaaa aatggctggg ttgagcgaag gcaagaggga
     6181 tttcatcatc atctttataa ggatggtgtt agaatcactg ttcctgttca tgccaatcaa
     6241 gatcttggcc gaggacttga gcgaaaaatt ttaaaggatg cgggattgaa ataatctcgc
     6301 attcgattaa agaaaggatt gactgaaatg ttaagtgagc ctttagcaaa aacctatcct
     6361 gctattttta gcccagaaga aggtggtggg tactttattg aatttcctga tgttcaaggt
     6421 gcttacacag gaattaacga agacgatata tcttatggaa ttgctatggc acaagaggtg
     6481 ttaggtatgg ttttagcaga ttatattgag catgaagatc tacttccaga accaacacca
     6541 atcaataaga tttctgttga agatgattct tttactactt taattcgtgt agatgttgcc
     6601 aaatatttga aagacacaga acttgtcaaa aaaaccttaa caattcctca atgggcggat
     6661 aagctaggca aaagggctgg cataaatttt tcagtccttc taacagaatc aattgcagat
     6721 aaagcagata acattcttcg ttctggaaga aataattaga actcaaaagg gtcagaaact
     6781 taaaaatctt tggcttcttt ttttactaca aagatggctt ctttaaatta tttgcctgat
     6841 tgatttaaaa aaattgcgca aagactacga gctaattaag ttgctcgtgg tcttttttta
     6901 tgaaaacttt ttagatctta ttatttgtca aattaagcga gacaaaactt tccatttacg
     6961 taactcaaaa aaacttctaa tggtgtttgg taatttagtg attttctagg gatatggttt
     7021 cttttggacg caacggatga gatgaatcct tgattgactt ggttgaagtc catttctttt
     7081 ggtagtccat cttttcggag gagtccgttt gaatgttcat ttaatccccg ttgggaaggc
     7141 gttcctgggt ctgagaaata aatatcaata tcattcgtat tactaatact tttccaacta
     7201 gagaattctt ttccacaatc aaaggtaatt gatttgaaaa gatttttggg tacggattga
     7261 agccattcat taatcgaatt ttcaatatca actaccttac ggccttctgg tttc
//
LOCUS       DAPBCS010000084         6643 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPBCS010000084 DAPBCS010000000
VERSION     DAPBCS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.087"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..859)
                     /locus_tag="RD521_002679"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="RD521_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="RD521_002680"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="RD521_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDT7772342.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="RD521_002681"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="RD521_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="RD521_002682"
     CDS             complement(2143..2373)
                     /locus_tag="RD521_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDT7772343.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="RD521_002683"
     CDS             complement(2603..3421)
                     /locus_tag="RD521_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDT7772344.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="RD521_002684"
     CDS             3582..4271
                     /locus_tag="RD521_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDT7772345.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="RD521_002685"
     CDS             4285..5787
                     /locus_tag="RD521_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7772346.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="RD521_002686"
     CDS             5800..6276
                     /locus_tag="RD521_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7772347.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="RD521_002687"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="RD521_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAPBCS010000085         6173 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPBCS010000085 DAPBCS010000000
VERSION     DAPBCS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.088"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            725..811
                     /locus_tag="RD521_002688"
     CDS             725..811
                     /locus_tag="RD521_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDT7772348.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="RD521_002689"
     CDS             complement(932..1621)
                     /locus_tag="RD521_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772349.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="RD521_002690"
     CDS             complement(1628..1906)
                     /locus_tag="RD521_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772350.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="RD521_002691"
     CDS             complement(1866..2780)
                     /locus_tag="RD521_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDT7772351.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="RD521_002692"
     CDS             complement(2762..3142)
                     /locus_tag="RD521_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDT7772352.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="RD521_002693"
     CDS             complement(3592..3879)
                     /locus_tag="RD521_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDT7772353.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="RD521_002694"
     CDS             complement(3899..4123)
                     /locus_tag="RD521_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDT7772354.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="RD521_002695"
     CDS             complement(4578..4793)
                     /locus_tag="RD521_002695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772355.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="RD521_002696"
     CDS             complement(4763..5296)
                     /locus_tag="RD521_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDT7772356.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="RD521_002697"
     CDS             complement(5317..6159)
                     /locus_tag="RD521_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7772357.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtcgatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAPBCS010000086         5897 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPBCS010000086 DAPBCS010000000
VERSION     DAPBCS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.089"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(976..1149)
                     /locus_tag="RD521_002698"
     CDS             complement(976..1149)
                     /locus_tag="RD521_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772358.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(1152..1550)
                     /locus_tag="RD521_002699"
     CDS             complement(1152..1550)
                     /locus_tag="RD521_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772359.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1766..2821
                     /locus_tag="RD521_002700"
     CDS             1766..2821
                     /locus_tag="RD521_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDT7772360.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2841..3194
                     /locus_tag="RD521_002701"
     CDS             2841..3194
                     /locus_tag="RD521_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772361.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3376..3672)
                     /locus_tag="RD521_002702"
     CDS             complement(3376..3672)
                     /locus_tag="RD521_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772362.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3676..4494)
                     /locus_tag="RD521_002703"
     CDS             complement(3676..4494)
                     /locus_tag="RD521_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7772363.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4720..4941
                     /locus_tag="RD521_002704"
     CDS             4720..4941
                     /locus_tag="RD521_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDT7772364.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4943..5209
                     /locus_tag="RD521_002705"
     CDS             4943..5209
                     /locus_tag="RD521_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7772365.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5322..5861
                     /locus_tag="RD521_002706"
     CDS             5322..5861
                     /locus_tag="RD521_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772366.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2065 a          982 c          874 g         1976 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaatcaa aatttaaatg ttttaatatt acaaataaaa tcatgttatt
      541 gtaaaatggt ataaaaaagc taaatattct atttttgtta ttttattagc aggaaaattt
      601 aaatagtttt acaagaatcc gtagaaatat attggttttg ccttcagatt aatactggat
      661 catattaatc tgaatataat atatgaacta ataaatagct taacctcgct attttgtttt
      721 ttctgatcac tatctataca gcattagtaa atagaatttt aaaaataaaa aaattaagac
      781 aaaaaagtat caacataaac atatacatat acacgtttat gttgattcac ttaatttgtc
      841 ctttaaaaag cacttatttt aatataatgt tattttataa tcttattaca agctgattgt
      901 taggcgttgt cctttcatta catttattaa cggttatttt attgtttaat ttattaatta
      961 tttttgatct aaaacttagc ttaacaaata aataattaac caaccgccag ataaaaatgc
     1021 gatcattgct aatacataag caaagataaa aatatgttcg atcagcttaa accctgatgc
     1081 gattaagcag accaagtaaa atagaaattc tcttacagat aatggtaatt ttctaactag
     1141 catactcata attaacaccc caaagtaata ttgtattttg aacattttac atccaataca
     1201 gcatcaatca accattgcca atcaaaaata caaggcttca ttgaagtatg tttttcagat
     1261 gaaaagatat aatataattt cttcggaata atattttcag ttgttgggat caagctaaat
     1321 ctaataccgt ctaactcata gctaccagct ttctctccat taaaaatttc cttagcactt
     1381 tcttctaaag aatcaaaata tttttcgaca gcaatctttg aagcttttac tattgaagca
     1441 tttccctttt cgataaaatc tgcttttgtt ttaccgacaa taagtaattc ttgatcagta
     1501 aaatcttcta aaataatggt agtgtaattc ttatttcttc tcaatcgcat caggatcact
     1561 ccttaatata atttatgttt ttataatatc ataataagat aaatttgcaa gcatttattt
     1621 acaccttttt atttacactt tattataatt aattagactt ttatgattgc atttttttct
     1681 tataaacagt tgcaactaat agtaaatagg tgtaaactat tttcgtaccg aaataccata
     1741 aacacaaaca gaaagaggtc ataccatgaa agaaccatta ttgttttccg ttgacttagg
     1801 gaataaatca gtaaaagaaa tgagtacaca cgtttctcca ctctcacttc cctctcgtat
     1861 tttgaacaaa aatatcatta attcaggacg tttttctaat ataggttcct cattaaaacg
     1921 atcactaaac gtttctgaat atagcctagt aaacactact gaaagctatt actttgggaa
     1981 ggatatacac gagctaggaa aagatgacta cgtgatagaa tcactaggtt ttgggatcag
     2041 cagatacaag caggaaacct accaaaatat cctatctttc tctttagcta acctagcacg
     2101 tttagaagat caatctgatg acgttttaac cgctaatcta gttttaggtc tgccttcatc
     2161 cgactatacc gaaacaatcg tcaaatacgt gatgcaacaa gccacacgac aacattcagt
     2221 tgaagttgac ggtaaagctt ataatataaa cgtagatacc gtttgggtac tgccacagcc
     2281 gattggaaca ctatataacc tcctacttgc tgatgatggt agtgtacaag atgaaacatt
     2341 gatcagtcag aagatcggtt ttgttgatat tggtggtggc acgaaacttt ttgatgttgt
     2401 ccaaaatttt gaaattgacg aaaacaaccg tgaacaaaag gataccggta tttttacttt
     2461 atataatcgt atcgtaaata tgcttgacct tccgatcagt cctaatgctt tccaacttga
     2521 aaagattatc cgagaaggca aagaaaaagg cgtatatcta tttaaaccaa gcggaaatga
     2581 aagttttgat atttcagaag tagttgaaag agaaataaaa acatacacca atatggttgt
     2641 tgcagaagtt ttagctacat tcaaaaactt cacaacctta gataaagttg tctttactgg
     2701 tggtggggct tctattatcg acaaacaact tgtaaaagat aaaattccgc ataccttgtt
     2761 tgttgaagac agtgaatttt ctaatgtaaa aggcttttac aagtttggtt taactcaata
     2821 aaacagcgaa ggagatcaac atgaataagg gaaaatcaga gcgttatgta gttataatca
     2881 atagagaaaa ggttagcgag ttacctttag cagagtttct tgaatctgaa tatgaaacac
     2941 taggcgttaa atactctcag acgatcaaaa aagctttgaa tctatatcgg cgtgtaaaga
     3001 acaatgaagt tacaattatt gaagcaggca acgatagaaa cgatagcaaa gtagctgaag
     3061 taaagaataa accaactgat cataacccta ttcaacaagc gcatccttca acggttacat
     3121 ccgatcattc agcaaagaaa gaaaagccaa tgctaaaagg gttcaatgca ccaagtaaca
     3181 aagacttact ttaaaaataa aaaggcgagg ttacaatacc tagccttttt attatgtaca
     3241 aatacaaaca taaacatata cgtatacatg tatataaaca tgtatacgta tatatccata
     3301 aacacgtata cgaaatttag ttaagctata cctaaccgct tattccaata aaaaaagtga
     3361 tcagctttaa actgatcact caccaaaaac ttcatcatac aagttatcta tcatttcacc
     3421 aagtaaagct gaatctgatc gaattcctaa cttttcgctg attttagata gtttctttct
     3481 ttgtgatggt tgaagcgtga attgataatt tttcttcttt tcagaactat cactttttag
     3541 tttagctacc aaagaacttg ttgtttcttt aggtgtttgt ttaggttctt caactgcttc
     3601 aaccacttca ggaacttctt ctacttcagc ttcagcgaat tttttaaatc tattttcttt
     3661 cacttcaatc actccttatt cttgtgccgt atcaattatt ttgttgtata gcgtaaacgt
     3721 gctttcaaag aaatccttat tcttacgata gatattctta tccttagcga actcagcaat
     3781 attcttacct tctaagctag ttgcattaag caattccttc tcttgtacat atccaagata
     3841 attagataac gttggtacca attctaggaa ctcacgactt gaattagtat tatgtttgat
     3901 cctgctaccg atcgtagcgt atttagtttc catcagtgtt tcaccagtct taatatcaat
     3961 caactgtttt gctagtgcat caatatcttt accaagctta agcaaagctt caaaaccatc
     4021 tttagatgga tcagaaatgc ctacgacaag atcagataca gcccaagcat tttgagctag
     4081 tagacctgca tcattatgag tatcaatcag aattagatca tacttctctt taagctgttc
     4141 atagttaaca acgatccatc taaacagtag ggttctgttg ttaggcatat cacgcatttg
     4201 catctcaact tgttttaaat cttcacctgc tactaaaata tccatgttct catttatagc
     4261 taaaggttca gctgatccat gaagatataa atgattgatt gtattctctc ttttcgcttt
     4321 actgaaagca tcaggaaatc gttttgatag gttttgcgac ttatcttcat caatatacag
     4381 caccttcata cctaaatttt cgacagcata tccacctaga ttgtagattg ttgttgtttt
     4441 acctgttcca cctttttcaa tgtttccagt aatgattgac aacttctttt ccatgattaa
     4501 acctccctta ttaatctttt gtaactccat tatacactat aaacacgttt atgtatatat
     4561 ttatatacat aaacgtgttt atatttatat ttgcttttat attctacaac ataaacatat
     4621 atatgtatat gtttatgttt atgtatatat tcaaatatat aactattgca tttgtcatac
     4681 aatcgtagta caatgtaatt gaaaaggagt tgataaacta tgccagtaat aagtattcgt
     4741 gtatctaaag aagaaaaaga ttttcttgaa aaagttgctg aattcaacgg agataacatt
     4801 tctgaattta tgcgtagtaa ttccctgcaa acagcagaaa cgttagtaga ctttgccacc
     4861 tacaagcaac taatgaaaga gcatgaggaa aacgatcaaa gtatttccca cgaagatatg
     4921 ctaaaagagt taggcttata agatgactta ccaagtaaga tatgaacgtg aagctcaaaa
     4981 agcgctaaaa aaaatggata agtttcaggc aaaggttatc ctaaactgga tcgagaaaaa
     5041 tcttgtaggc actgatgatc caagaaagca tggtaaaggt ctaacagcta ataaatcagg
     5101 ctactggcga tatagggtag gtgcataccg tataatagct gatattagcg agcatgaagt
     5161 gacaattctt atattgtcta ttggtcacag aagtgaagtt tataagtaac agacaagctg
     5221 ataaggcttg tctgtttttt tgttaaccca acaaaatcac tgtttacgat cccactttac
     5281 ctaaaaaaga acaatatcct cttgatatta tacacaccag tgtgtataat ataagtgtaa
     5341 acaagctaat taactttcaa aaggagcgat tttttatggg atggtatggt gattattcat
     5401 cagcaaatga agtgatcgaa gaaaagaaag ggtacgccaa aaatggtttg gaaatcgtag
     5461 gtaccaaagt aacgaagacc tatggaatta tccttttcaa agatgcagaa ggaacctacg
     5521 atattgacag cttcatcttt cgagatgaaa tgtacaaacc aatctacttc ttaaatgaca
     5581 gtcctgctta ctttaagaaa attccgaaaa aatggttgaa attctttgtc ggtgatcaag
     5641 aaaaaatggt taagctacaa ctagatagaa ttaaagaaga aagcaataaa ccaaaacttg
     5701 atgacttatt agttgcaggt aaaacttact caatctggga tggatcgtat gtcgctgtct
     5761 atcagtataa actcaaacgt agctttattt ttcgactaca agatggatca ctcactcggt
     5821 tcactcgact atcagcagga gatgttaagg aagtaaccta aaacttaaat acataaacat
     5881 aaacgtgtat acgttta
//
LOCUS       DAPBCS010000087         5874 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPBCS010000087 DAPBCS010000000
VERSION     DAPBCS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.090"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            196..921
                     /locus_tag="RD521_002707"
     CDS             196..921
                     /locus_tag="RD521_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772367.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="RD521_002708"
     CDS             911..1420
                     /locus_tag="RD521_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772368.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="RD521_002709"
     CDS             complement(1490..2938)
                     /locus_tag="RD521_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7772369.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="RD521_002710"
     CDS             complement(2938..3654)
                     /locus_tag="RD521_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7772370.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="RD521_002711"
     CDS             complement(3635..3985)
                     /locus_tag="RD521_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDT7772371.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="RD521_002712"
     CDS             4129..4902
                     /locus_tag="RD521_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDT7772372.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="RD521_002713"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="RD521_002713"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5744..>5874)
                     /locus_tag="RD521_002714"
     CDS             complement(5744..>5874)
                     /locus_tag="RD521_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7772373.1"
                     /translation="QVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1706 a         1143 c         1023 g         2002 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaatt attatttgtc aaattaagcg agacaaaact
     5761 ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt gattttctag
     5821 ggatatggtt tcttttggac gcaacggatg agatgaatcc ttgattgact tggt
//
LOCUS       DAPBCS010000088         5769 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPBCS010000088 DAPBCS010000000
VERSION     DAPBCS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.091"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..2376
                     /locus_tag="RD521_002715"
     CDS             4..2376
                     /locus_tag="RD521_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772374.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="RD521_002716"
     CDS             2437..3897
                     /locus_tag="RD521_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDT7772375.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..>5769
                     /gene="topB"
                     /locus_tag="RD521_002717"
     CDS             3908..>5769
                     /gene="topB"
                     /locus_tag="RD521_002717"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDT7772376.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQ"
BASE COUNT         2186 a          987 c         1260 g         1336 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagta gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaccccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat caaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttcttctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagcg
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttata
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccac gaacagacac cccttatatt acggaagggg agtatgcgta
     4921 tttgttggat catttagatg aatacaagca ttttttaaag gccgaatcaa tcccaacccc
     4981 tattcatacg ccgaatagtc ggtatgtgaa taacaagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggccatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcgaaaaggt
     5221 gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg atctacaagt
     5341 tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcaat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc tttgccaagc
     5581 cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata tccaaaaatt
     5701 tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt ttggatcgac
     5761 gttacaaga
//
LOCUS       DAPBCS010000089         5691 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPBCS010000089 DAPBCS010000000
VERSION     DAPBCS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.092"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            58..510
                     /locus_tag="RD521_002718"
     CDS             58..510
                     /locus_tag="RD521_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772377.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="RD521_002719"
     CDS             524..1213
                     /locus_tag="RD521_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772378.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="RD521_002720"
     CDS             1338..3689
                     /locus_tag="RD521_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDT7772379.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="RD521_002721"
     CDS             3769..4164
                     /locus_tag="RD521_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772380.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="RD521_002722"
     CDS             4489..5106
                     /locus_tag="RD521_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772381.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="RD521_002723"
     CDS             5147..5446
                     /locus_tag="RD521_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDT7772382.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..5647
                     /locus_tag="RD521_002724"
     CDS             5480..5647
                     /locus_tag="RD521_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7772383.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
BASE COUNT         1996 a          751 c         1161 g         1783 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     5641 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt t
//
LOCUS       DAPBCS010000090         5689 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPBCS010000090 DAPBCS010000000
VERSION     DAPBCS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.093"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(488..1297)
                     /locus_tag="RD521_002725"
     CDS             complement(488..1297)
                     /locus_tag="RD521_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDT7772384.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="RD521_002726"
     CDS             complement(1297..2043)
                     /locus_tag="RD521_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDT7772385.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="RD521_002727"
     CDS             complement(2061..2528)
                     /locus_tag="RD521_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDT7772386.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="RD521_002728"
     CDS             complement(2528..2938)
                     /locus_tag="RD521_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDT7772387.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="RD521_002729"
     CDS             complement(2949..3962)
                     /locus_tag="RD521_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7772388.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="RD521_002730"
     CDS             4179..4913
                     /locus_tag="RD521_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7772389.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="RD521_002731"
     CDS             4897..5607
                     /locus_tag="RD521_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7772390.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAPBCS010000091         5486 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPBCS010000091 DAPBCS010000000
VERSION     DAPBCS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.094"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            108..668
                     /locus_tag="RD521_002732"
     CDS             108..668
                     /locus_tag="RD521_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDT7772391.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNKTHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1576..2343
                     /locus_tag="RD521_002733"
     CDS             1576..2343
                     /locus_tag="RD521_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDT7772392.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2369..2914
                     /locus_tag="RD521_002734"
     CDS             2369..2914
                     /locus_tag="RD521_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772393.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            2915..3481
                     /locus_tag="RD521_002735"
     CDS             2915..3481
                     /locus_tag="RD521_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772394.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            3496..3744
                     /locus_tag="RD521_002736"
     CDS             3496..3744
                     /locus_tag="RD521_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772395.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            3764..4003
                     /locus_tag="RD521_002737"
     CDS             3764..4003
                     /locus_tag="RD521_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772396.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4374..4655
                     /locus_tag="RD521_002738"
     CDS             4374..4655
                     /locus_tag="RD521_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDT7772397.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4645..4986
                     /locus_tag="RD521_002739"
     CDS             4645..4986
                     /locus_tag="RD521_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDT7772398.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
BASE COUNT         2049 a          751 c         1106 g         1580 t
ORIGIN      
        1 tttatactct cctggaagca atcaatttgt taattctgat agtagtatca tatgtttaga
       61 aaaagtatta aaaaaaatag ctttataaag ttttatggag gaacaacatg ggagacgaaa
      121 aaaagcgctt ggaagttcaa ttacgtgaag catatggacg agtaacgtat acttacacca
      181 ctcatttaaa gcaagccgat aatattattt ctgacaataa aattttaagg tgtcttcaaa
      241 ttggtttgtc agcccttact actggagggg tacttagtac cattattact gataatagtg
      301 agttaacctt tgttgctgga ttatgttcaa caatttctct tggattaaac ttatacttta
      361 agaattttga tcttgctgaa gaagctaaaa atcatcaaat tgttgccgat aaattatggt
      421 tgatcagaga agaatacgta agcttactaa cagatttaaa tcgtttaagt gttgaagata
      481 ttgtgaaaaa aagagatcaa ttaatgaaca aaactcatga agcatataaa aatgccccta
      541 aaacaaatag taaaagctat gaatcagctc agaaagcatt aaaatctgaa gaagagcaat
      601 tttttacaga agaggaattg gataagatgt taccagagca tcttagaaaa aagagttcta
      661 aatattgaac tcttttttta tgcttaaaat tttcttgtaa tttttctatt caatcaataa
      721 ataatctata tttaagaaga aatatgatga tagaaacatc tcctaaagcc accgcaagcc
      781 gtcctagcca ttgcccagtc cgacatcgct agattagtgc atctctccaa aaaagttttc
      841 taaagccacc gcaagccgtc ctagccattg cctagtccga catcgctaga ttagtgcatc
      901 tctccataaa agttttctaa agccaccgca agccgttcta agcatagctt agcccgacat
      961 tgctagatat gctcatctca ccatgaacgt ttcctaaagc caccgcaaac cgtcctagcc
     1021 atcgcctagt gcctagtccg attttgcttg atcggttcca tttctgttta tttagtgggt
     1081 caacaccgta atcttaaata gccatcgcct atttaagatt actaggtctg ctttgaaggt
     1141 aaaaagtctt tcattcttca agacttttag gctttcaagc tacgcctgaa agcattgaaa
     1201 ttcatcgctc tttatttggt tttgccattt ataattcctt acgcccatta tctttttcgc
     1261 ctgtcatacc ttgtcagaag tgtttagagc ttatctcatc tttggattaa tacaagtggc
     1321 gtaaattttt ttctaaacgc aagcgttacg aaaataattt acttaaacac ttgtattaat
     1381 ccggcagata agaacgcatt ctaatcactc ccgacaaggc aagacaggcg aaaaagataa
     1441 ataaatgaac gaaaggaaga aacataaaat gcaaaaccaa atagcgatga agaaaaaacc
     1501 acctccctct aaaacaatcc aaacaaaaaa gaattttgaa aaaccaaaaa tcaaaaacag
     1561 aaagaagcgt tcaacatgag agatagaatt ttagaagtta atcaagaaat ggtaaacaag
     1621 ttaacaaata tcagtgggaa ctatgaaaag tacgatggta ctttacttga taattatatt
     1681 tttcacgata cacaaaatat cagaattggc agaagtaaac cgagagcgtt cattctcatc
     1741 aaagagaaat atttgaatga atggaatagc acctatgaaa tggttatgac tgataacttt
     1801 gaaaaggtat tggatttcag aaatgctttg ttgggttctg atatgggaaa tagcagtctt
     1861 gaatctattg aagttgaaaa caagaccgag aatgaaggtg aaggattgat taagaatgtc
     1921 ctgaaaatcg aacaaaaata tatcagtgaa gatagaagtt tttatagagt cacaagctcg
     1981 gttggtctag caaactttgc tactaactta attggaaaca atgctagtga atctttgcta
     2041 gtgatgtgtc tgaatacaaa gaatgaggtc attgcttata gcgaagtttt caaaggtgga
     2101 attaatcaat cagtagcgat accaagagag atttttcaac tagctttgct aaacaattca
     2161 gcaaaaatcg cagtatcaca taatcatccg agcggatatg aatttccaag tgaaaatgac
     2221 aaaagtttta ctacgaattt gaaaaacgca ggaaaaacaa ttggtattga attgttagac
     2281 catatcatcg taacaagtaa aaacacaagc tattactcat ttcaagaaaa tggttcttta
     2341 taaaaattag agaaaagagg aaaacaagat gggcgaaaga acacaagcgt taattacagt
     2401 caaatatggt aacagcaaaa cgaaagtaag cgtgtcaatt cattatcagt ggggatatgg
     2461 tcgattcatg ttgatggacg ttttgaatca aggaatatat ttaatcaata aagcatatga
     2521 cttacgagat gaatcaagag aagaattagc aaaaggaatc tttgatagaa gtacaggagt
     2581 tatcaatttt gtcacctatg aatctggagt gagtgcttat gatgaaacag ggtttggtgg
     2641 gcgctatgaa ccttatcaag aaacgattga agatgtgatg aagttttcag ataacaatga
     2701 tggctatgct catttagaga tggtgttcga tgaaaatgga tttagtctag atgataaaac
     2761 cagattgcga ttgtttgatt ttgagggtca acagatagga ctaaacaagt tttgtgaaag
     2821 cggaaaagag tttgctacga aagattttat taagggatac agattattgc tagaaaatta
     2881 tggaataaac ctagaagaag agagcgtgaa ataagtgttt gaaaaaagaa aagaccagtt
     2941 ccctaattta gaagcgaaaa gtcaaaaagt gattgatggt gaaatgattg aattattcca
     3001 aaaaagttat ggaaccatag aaactaccgc aagttgcaaa tactactttc aaggaaagag
     3061 aaacacgttt aaaggctttg ccgattctga tgaagaattg gcaaagaaaa tcattgattg
     3121 gcttgtcagc agtgaacacg tagcaagaga agataccgtt agaacaacga ttgagggtga
     3181 agtcctaaca ggtactagat gtgtttgttg taatcatgaa tttgtagaag atgaaggttg
     3241 ctaccgaatc aatgaatatg gtgattttat ttgccagagt tgccacgaga tagaatttcc
     3301 aaccgaaaga agttggtttg aatacatgct acaagattat gagatggaaa cagaaaatgg
     3361 tgaagtagtt ttttgtacca aagaggaatt gagtaagaga agcgatgatg aaattgaaga
     3421 ttggtgtgtg tggttagtag ggacagatga ttgcgttgca tattacacaa cagcagaata
     3481 ggagtgtgaa tcagcatgga aggaatggat tttctagatc atgaagattt ggttgatttt
     3541 ggttatacat ggaaaggaat ggtaggtatt agcagaagtc ttgctaatgc cttttacgaa
     3601 agaaattatg cagtatatgt tctttatgat gacgatacag aaagccttgt tgacgaggaa
     3661 tataaattgg atttagagaa tgttttatat ggaatagaaa aggaagattt agcaaaatat
     3721 attttttcat ggttgggtca ataagtgaaa ggaagataga aacatgacaa aatttaaaag
     3781 taagggtatg caatggtgtt cttattatta tgttggaaat gatgaagatt tattgagaaa
     3841 aacccaacga aaaggttatc cagataagat attgaaacaa ataggttatt acatgccaga
     3901 agagaggata gaaaaagaat tttgtgaggt tctttttgat agtgaacagc tattagtttt
     3961 gttaacccaa aataaattga tttgccaatt tgaaaataat taagatgagg tggtgaacca
     4021 tgaatgaaga taagatggta ttaagtgaat atgctagatt gagaactatt aattctttag
     4081 catctcattt aagttgttct ttcctaacaa aggtgctaaa attaatttac aaatgtgaag
     4141 attttgtaca agagaagcac aaagacaagc ctactgcgaa taggcttgtc tttttttcat
     4201 tcattacgat tccgtatgtg tgggttagcg acaatttgtt caattaccgt atgatatagc
     4261 atcttaattt gattagatta cttgcaatgt agtttattaa gagtataata taatcataaa
     4321 tgtggacaat ttgataaata atgagtgttt tatactcaaa aggagtggat gaaatgaagg
     4381 tcaaaaatcc aactgaaaaa agcagaatcc aagtcggaat agacaagaat ttaaaagaga
     4441 acgcagaaat gatattggaa gagctaggtt taaatcctac aacagcaata acaattcttt
     4501 acaaacaagt agtcgctaga ggtgagtttc cagtagagat aaagctaagt gaagaggaga
     4561 agcaaggaat cagattgcaa cagctaacta aggacatgcc agtagatgtt ctagatacag
     4621 atgaaaagtt agaagagtgg ttcgatgaag cctaatgaga tagtaacggt atatgttgca
     4681 tttacagata aaaatggtgg taaaagaaga cctattttgg tggttagtga taaagaagat
     4741 agagtagaat tctttggaat cacaagtcaa tatgagaaaa aatcagacca aataaagagg
     4801 gtgtattttc ctattgcaga atgggaaaaa gcaggactta aaaagcaatc atggattgac
     4861 gtagggtctt taaaggctat tcccaaaagc cgagagaaca tatcattcaa aaaggttggt
     4921 atgttatcag ctagtgatac gaaaaagcta aataggttta tcgagaaatt acaaagaaaa
     4981 gtctaacaag gcttttcttt tttgtttata tgtatattaa gatatacgtt tatataaacg
     5041 tatttatgta tatgaaaata tacgtaaata tatacgtggt tataaacatt aataaaacgt
     5101 taagaattgt taaataatga tatttagtaa tatacgaatt ataatatata ttttatataa
     5161 aatgtatgta aataaataaa atgtctgacg gatattgaat agcaataaat aacaataatt
     5221 cagacggata cggtgagtga agtagcttgc tacgaacgaa caggcagaat gaaacggcag
     5281 tttcattaga gcaagtcgct tgcgacgtag cgttaccgct gatgtaactg gcaagttaca
     5341 tagagagagt ttgcttgcaa acgaacgtta cctatttgaa tagaaataaa aaataacaga
     5401 ttagtgaatt ttgaatagga gccacaactc aaccaaccgc ttgcggtggg tgagtcccac
     5461 ggaggaaaag acgaacgaac ggtcgc
//
LOCUS       DAPBCS010000092         5456 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPBCS010000092 DAPBCS010000000
VERSION     DAPBCS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.095"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..556
                     /locus_tag="RD521_002740"
     CDS             <1..556
                     /locus_tag="RD521_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HDT7772399.1"
                     /translation="NLIRGEFHFLSEKDMNIIPSFKDSGYIPDTKAMLIYDETGQLEN
                     YLSEISGARPNRLTEENANFLLCNFSNLWLMGINVLKRGEYARSLELLSQLQKNTLQL
                     IRMAEKNADNWLNMSKNLEKEISLENYKKFAKTTARLDKVELFEAYKNSLLLVMDLQS
                     HLIEQYNLKVTHDILERLLNYISE"
     gene            1244..2065
                     /locus_tag="RD521_002741"
                     /pseudo
     CDS             1244..2065
                     /locus_tag="RD521_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
     gene            2208..2432
                     /locus_tag="RD521_002742"
     CDS             2208..2432
                     /locus_tag="RD521_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7772400.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            2447..3316
                     /locus_tag="RD521_002743"
     CDS             2447..3316
                     /locus_tag="RD521_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7772401.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3297..4031
                     /locus_tag="RD521_002744"
     CDS             3297..4031
                     /locus_tag="RD521_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7772402.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4064..4972
                     /locus_tag="RD521_002745"
     CDS             4064..4972
                     /locus_tag="RD521_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDT7772403.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            4969..5217
                     /locus_tag="RD521_002746"
     CDS             4969..5217
                     /locus_tag="RD521_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HDT7772404.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(5220..>5456)
                     /locus_tag="RD521_002747"
     CDS             complement(5220..>5456)
                     /locus_tag="RD521_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7772405.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         1865 a          821 c         1159 g         1611 t
ORIGIN      
        1 taatcttata cgtggggaat ttcatttcct ttctgaaaaa gatatgaaca taatcccctc
       61 gtttaaagat tcaggttata ttcctgatac gaaggctatg cttatttacg atgaaacagg
      121 gcaattagaa aattatttat cagagataag tggtgcaaga ccaaatagac ttactgaaga
      181 aaatgctaat tttttgttgt gtaatttctc taatctatgg ttgatgggaa tcaacgttct
      241 aaaaagagga gaatatgctc gttcattaga actcttatca caacttcaaa aaaatacact
      301 acaacttata cgtatggcag aaaaaaatgc tgataattgg ctaaacatga gtaaaaacct
      361 tgaaaaagaa attagccttg aaaattataa aaaatttgca aagaccactg ctcgattaga
      421 taaggtagaa ttatttgaag cctataaaaa ttctttgcta ttagttatgg atttgcaaag
      481 tcaccttatt gaacaataca acttaaaagt tacacatgac attttagaaa gattgttgaa
      541 ttacattagt gaataggaga gtaacgttat tcacagtatt gggtactgaa aattaacagc
      601 atacctcagt ttttcaaaaa acagcgaagc ctatggatat gttcccgtct accgatagtg
      661 caaaaagtgt cgtggatatg tttccgagaa cggacataca caaatgacat tcattctgtc
      721 ctctcaatcc atgtggaagc agtatgtctg ttaacaagaa aataattatt gttcgtcctg
      781 acgagttgtt agactcgaaa aggataaaca tcatttttag aaccggtagc cgatttttgg
      841 ctagcggttt tttgggctct ttggcaaata gtggtgattg aatctcgcgc tatcctatgg
      901 aaaaccgcct tgtgtgaggg gctgcgagac cgcttcgatc ctagaattgc tgaagcacga
      961 atctagcgtc actcacattt catcttttga cgtagctgag acaaggtgtt tttcttcgat
     1021 ccgctagtgg ttaatctctt tttggtgcaa atatggttta gaatttcttt gcatggctta
     1081 agggagtgga gaatcggata ttagtaggat ttacatccac agataaaaat aggcttgatt
     1141 ttaacagcga aactaaaata ccctacttaa atgaagtgat agattagatg ttgggttgtt
     1201 aggagatgct taaaaatcct atgcaatcaa tagtttttag tgtttgttga cttgatccct
     1261 aaatcgcctg agtactaaac gttgagtgtg tctgcttact gtcactagcc agtaagtgag
     1321 actgtagtta atctctcatt ttccccagta ttatgttgaa aatagccatg aagcgattat
     1381 ttctaaagag ttatttttgc aggctcaaga agaaatttat cggagaagta atatctacac
     1441 aggagaaggc aagaacaaaa gaatttatag tagcaagtac gctttaagtg ccatcacctt
     1501 ctgtggatat tgtggcgata tttataggag aacctattgg aatattcatg gcagaaaaga
     1561 atttgtctgg cgatgcgtga ctagaatcga gcagggtccc gaggtatgta agaaccgaac
     1621 cgtaaaagaa gatgaactct atggtgcggt aatgaccgca attaataagc tacttgctgg
     1681 tggcaacaat atgataaaga cactggaaga aaatattcat gctgtgattg gagaaaccac
     1741 agaataccaa atttcagaga ttaataccct actagatgaa aaacaaaaag aactaatcaa
     1801 gctggccaat aaaggtcaag attatgaata cctggtagat gagatcgatg agatgcgaga
     1861 taagcgtcag actcttttag tagaagatgc ctccctcagt ggcgagaacg agcgcatcaa
     1921 tgagttgatt gaatttatcc gcaagaacaa attccgcacc ttagaatatg atgataagct
     1981 tgtaaggaag ataatccaga acgttaaggt ctatgaagac cacttcgtca tagccttcaa
     2041 accaggtatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgac tctgaagtag
     2101 agttgtgggt tttcttttaa ttgctgatta taataatatt ttaatgacta ttgcatttat
     2161 caaccaaatg gtttatgtta ttcttagcat aaacttggag gtgctgtatg actacggcag
     2221 aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt gctagacgta
     2281 ttggccagac tccacagaat tttaataaaa aattacaacg agaaacagta accttggatg
     2341 agttgaaggc cattgctgat gtgctaggtg tcaagtttgt gcaggcattt attttaccag
     2401 atggagatga aataaaaata tctaacgaat aaaggaggat gtttatatgg taggtaatgt
     2461 tattcaaata gtaacagaga aattatcatc tttgcctttt atagaaggca ttgtattagg
     2521 gggctcacgt gcaagaagca cccatacaga aaattctgat atagatatcg gaatctatta
     2581 caattcagac tcatttgacc tgacagcgat taatcaaatt gctacagagt tggatgatga
     2641 gaatagaaac aaccttgttg tacctcccgg agcgtggggt gattgggtta atggcggtgg
     2701 atggttagtt ataaacgggt gtcatgttga cttgatatta cgtgatataa aacgggtgga
     2761 acaaataatc aaagatacgg agcaaggaat tgttactgcc aattatcaga ccgggcatcc
     2821 ccatggttat ataagtgcta tgtatcgtgg agaattagcg attagtaaaa tactatatgc
     2881 taagaacgaa agcttatgcg aattaaaaaa gcaggcagaa atttacccta ctgctctaaa
     2941 gaaaagcttg atgaactttt ttatatttga agctgaattc tctttaatgt ttgtaaaagc
     3001 aaatgcgggg gcagaggata aatattatat tgcaggccat gtttttcgta ttatttcatg
     3061 cttaaatcaa gtactatttg catgtaataa tgcttattgc attaacgaaa agaaagctat
     3121 aaaactgctt gaaacttttg aatataaacc tgaaaaatat gccgaaagag ttaatcatat
     3181 ttttgaagta ctcggtcttt ctctttttga atgctacgac atgaccgaga aactttataa
     3241 agaagtgaat gaaattgtat cggagataaa taacttttta aacgaggaga gttcagatga
     3301 aagaaaacaa atatgatgat aatatatttt ttcaaaaata cagtcaaatg agtcgctcgc
     3361 agaaaggact ggctggtgcg ggagaatggg agactttgaa aaagatgcta cctgatttta
     3421 agggtaagcg tgtgcttgat ttaggatgcg gctatggatg gcactgtata tatgcgatgg
     3481 aaaacggtgc ttcctctgta gtaggtgttg atatttctca taaaatgctc gaagtagcaa
     3541 aaggaaaaac ccattttcca cagattgaat atgaatgctg tgccatagaa gatgtggatt
     3601 tcccagagga gagctttgat gtaatactaa gttcgcttgc gtttcattat gtagcagact
     3661 atgagaattt aataaaaaag atatatagga tgctgaaggc tggtggcaat ttagttttta
     3721 cagttgaaca tcctgttttt actgctcatg gaacacaaga ctggtattat aacgaaaaag
     3781 gagaaatact gcatttcccg gtggacaatt attattatga gggcaaacgg acagctatgt
     3841 ttttggaaga aaaggttaca aaatatcata gaacactgac cacatatcta aatacactgc
     3901 tttcaaatag ttttataata aatcagattg tggagccaca gccgccagag aacatgatgg
     3961 atattccggg gatggcggat gaaatgcgac gcccaatgat gctgattgta tcggcaaaaa
     4021 agaagatgta ataatataga aaaaataaac gaggagtatg taaatgagat cagaaaaaga
     4081 aatgatggat ttagtacttt ctttagcaga acaggatgaa cgtattcgaa ttgtgaccct
     4141 tgaggggtca cgcgcaaata ttaatatacc taaagatgaa tttcaggatt atgatattac
     4201 atattttgta agtgatatag aaccgtttat atctaatgat gactggctta atcaatttgg
     4261 gaatataata atgatgcaaa agccggagga tatggaatta ttcccacctg aagaaaaggg
     4321 attttcctat cttatgctat ttgatgatta caataaaatt gatcttacct tattgccctt
     4381 ggaagagtta gataattacc taaagggcga taaattaata aaggttctaa ttgataaaga
     4441 ttgtagaatt aaaagggaca tagttccgac tgatatagat tatcatgtaa gaaagccaag
     4501 cgcaagggag tatgatgatt gctgcaatga attttggaat gtaacacctt atgttattaa
     4561 aggattgtgc cgtaaggaaa ttttatttgc tattgatcat tttaatcaga ttgttcgcca
     4621 tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta aattaagtgt
     4681 aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg agaaactttt
     4741 gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc tatgccatca
     4801 attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct atccggagta
     4861 tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg gtaaaaccgg
     4921 ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt gattacagaa
     4981 atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga ggtgataggt
     5041 aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact ctatgaagcg
     5101 ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc agattgcctt
     5161 gaatatattg acaatactga taagataata tatcttttaa cccgtggtgc tagttaattt
     5221 taatatccta aagaatatct caaagttcct ccaaaacgtc gttgtgctcg acttaataga
     5281 acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac agaacgtgag
     5341 ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac tgtttttctt
     5401 tgttttccaa gtaagctgtg gtctagtttt tctgatttat ccatgttttt acgtgg
//
LOCUS       DAPBCS010000093         5340 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPBCS010000093 DAPBCS010000000
VERSION     DAPBCS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.097"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2616)
                     /locus_tag="RD521_002748"
     CDS             complement(<1..2616)
                     /locus_tag="RD521_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDT7772406.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISD"
     gene            2762..3337
                     /locus_tag="RD521_002749"
     CDS             2762..3337
                     /locus_tag="RD521_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772407.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3551..4246
                     /gene="vanR"
                     /locus_tag="RD521_002750"
     CDS             3551..4246
                     /gene="vanR"
                     /locus_tag="RD521_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDT7772408.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4224..>5340
                     /locus_tag="RD521_002751"
     CDS             4224..>5340
                     /locus_tag="RD521_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7772409.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL"
BASE COUNT         1665 a         1009 c         1019 g         1647 t
ORIGIN      
        1 atccgatata taattcaaaa taaagatcgt tttttctatt cggcccatct cacgtaaggc
       61 tgtagctaag ctgttttgtc ttgaatagga acctagcttc cccataataa gggatgctga
      121 aactgttccc tcccttatag aatgagctaa tcgcaaaaca tcctcataat tttctttaat
      181 gacctttgta tttatttgtc cacgtaaaat ggcttctagt tttggatact cacttgcttt
      241 atctatcgta aataattttg agtccgataa atcccttatt cttggggcaa atttaaatcc
      301 taataaatga gtcagtccga atatttggtc agtgtaaccg gcagtgtctg tataatgttc
      361 ctctatgttt agatccgtct catgatgtaa caaaccatcc aaaacatgaa tcgcatctct
      421 tgaattagta tgaataatct ttgtgtagta agaagagaat tgatcacttg taaatcggta
      481 gatggtggct ccttttccag ttccataatg tggatttgca tctgcatgta gtgatgaaac
      541 acctagctgc attctcatac catctgacga agatgttgta ccgtcgcccc aatagaaagg
      601 caattgtaat ttatgatgaa agtttactaa tatggcttgg gctttattca tggcatcttc
      661 atacatgcgc cattgagata cattggctag ttgcttatat gtaagtccgg gtgtggcttc
      721 ggccatcttg ctcaagccaa tattcattcc cattcctaaa agggcagcca tgataatgat
      781 tgtttcttcc ttatctggtt ttcgattatt ggaagcatga gtgaattgct catgaaatcc
      841 tgttatatgg gccacatcca tgagtaaatc agttaatttt attcttggta gcatctgata
      901 aaggcttgca ctaaattttt ttgcttcttc tggaacatct ttttctaagc gtgcaagtga
      961 tagctttcct ttttcaagag aaaccccatc taacttattg gaattggcag ctaaccactt
     1021 taacctttca ttaaagctgc tggttctctc cgttatataa tcttcgaatg ataaactaac
     1081 tgataatctc gtattcccct tcgattgatt ccatgtatct tccgaaaaca aatattcctc
     1141 aaaatcccta tattgtctgc tgccaacaat ggaaacatct cctgcccgaa catgctcccg
     1201 aagttctgtt aaaacagcca tttcatagta atgacgatta attgttgtac catcatcctc
     1261 gtataaatgt cttttccatc gttttgaaat aaaatccaca ggtgagtcat caggcacttt
     1321 tcgctttcca gattcgttca ttcctcggat aatctcaaca gcttgtaaaa gtggctcatt
     1381 tgcctttgta gaatgaaatt ccaatactct taatagcgtt ggcgtatatt ttcttagtga
     1441 ataaaaccgt ttttgcagta agtctaaata atcatagtcg gcaggacgtg caagttcctg
     1501 agcctcttct actgaagaga caaaggtatt ccattcaata accgattcta aaaccttaaa
     1561 aacgtctaat ttttcctctc ttgctttaat taatgcttgt ccgatgttcg taaagtgtat
     1621 aactttctca tttagctttt taccgttttg tttctggatt tcctcttgag ccttacgacc
     1681 ttttgataac aaactaagta tttgcctatc atgaatttca aacgctttat ccgttagctc
     1741 ctgagtaagt tgtaataaat agatggttaa tatcgaataa cgtttatttt cttgaaagtc
     1801 acggaatgca tacggctcgt atcttgagcc taagcgagac agctgcaaca ggcggttacg
     1861 gtgcaaatga ctaatttgca ctgtttctaa atccattcct cgtatgtatt cgagtcgttc
     1921 tattattttt agaaaagttt cgggtgaagg atgacccggt ggctctttta accaacccaa
     1981 tatcgtttta ttggattcgg atggatgctg cgaggtaata atcccttcaa gcttttcttt
     2041 ttgctcattt gttagagatt tactaaccgt attaaatagc ttcttttcag ccattgccct
     2101 tgcttcccac accattcttt caagtgtagt gatagcaggc agtataattt tgttttttct
     2161 tagaaaatct atgcattcat gcagtagatg aatggcatca ccattttcca aagctaattg
     2221 atgaaggtac ttaaatgtca ttcgatattc actcagggta aaagttacaa agtcgtattc
     2281 acttcgaatt tctttcaaat gatcccaaag tgtattttcc ctttgaggat aatgatcaag
     2341 cgaggatgga ctaacaccaa tctgtttcga tatatattgt atgaccgaat ctgggatgct
     2401 tttgatatga gtgtatggcc aaccgggata ccgaagaaca gctaattgaa cagcaaatcc
     2461 taaacggttt tcttccctcc ttcgcttatt aactatttct aaatcccgtt tggaaaaagt
     2521 gaagtaggtc cccagtatcc attcatcttc agggatttgc ataaaagcct gtctctgttc
     2581 cggtgtaagc aattctctac ctctcgcaat tttcattcag tatcattcca tttctgtatt
     2641 ttcaatttat tagttcaatt atatatcaat agagtgtact ctattgatac aaatgtagta
     2701 gactgataaa atcatagtta agagcgtctc ataagacttg tctcaaaaat gaggtgatat
     2761 tttgcggaaa atcggttata ttcgtgtcag ttcgactaac cagaatcctt caagacaatt
     2821 tcagcagttg aacgagatcg gaatggatat tatatatgaa gagaaagttt caggagcaac
     2881 aaaggatcgc gagcaacttc aaaaagtgtt agacgattta caggaagatg acatcattta
     2941 tgttacagac ttaactcgaa tcactcgtag tacacaagat ctatttgaat taatcgataa
     3001 catacgagat aaaaaggcaa gtttaaaatc actaaaagat acatggcttg atttatcaga
     3061 agataatcca tacagccaat tcttaattac tgtaatggct ggtgttaacc aattagagcg
     3121 agatcttatt cggatgagac aacgtgaagg gattgaattg gctaagaaag aaggaaagtt
     3181 taaaggtcga ttaaagaagt atcataaaaa tcacgcagga atgaattatg cggtaaagct
     3241 atataaagaa ggaaatatga ctgtaaatca aatttgtgaa attactaatg tatctagggc
     3301 ttcattatac aggaaattat cagaagtgaa taattagcca ttctgtattc cgctaatggg
     3361 caatattttt aaagaagaaa aggaaactat aaaatattaa cagcctccta gcgatgccga
     3421 aaagcccttt gataaaaaaa gaatcatcat cttaagaaat tcttagtcat ttattatgta
     3481 aatgcttata aattcggccc tataatctga taaattatta agggcaaact tatgtgaaag
     3541 ggtgataact atgagcgata aaatacttat tgtggatgat gaacatgaaa ttgccgattt
     3601 ggttgaatta tacttaaaaa acgagaatta tacggttttc aaatactata ccgccaaaga
     3661 agcattggaa tgtatagaca agtctgagat tgaccttgcc atattggaca tcatgcttcc
     3721 cggcacaagc ggccttacta tctgtcaaaa aataagggac aagcacacct atccgattat
     3781 catgctgacc gggaaagata cagaggtaga taaaattaca gggttaacaa tcggcgcgga
     3841 tgattatata acgaagccct ttcgcccact ggagttaatt gctcgggtaa aggcccagtt
     3901 gcgccgatac aaaaaattca gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc
     3961 cggccttgtc attaatgtta acacccatga gtgttatctg aacgagaagc agttatccct
     4021 tactcccacc gagttttcaa tactgcgaat cctctgtgaa aacaagggga atgtggttag
     4081 ctccgagctg ctatttcatg agatatgggg cgacgaatat ttcagcaaga gcaacaacac
     4141 catcaccgtg catatccggc atttgcgcga aaaaatgaac gacaccattg ataatccgaa
     4201 atatataaaa acggtatggg gggttggtta taaaattgaa aaataaaaaa aacgactatt
     4261 ccaaactaga acgaaaactt tacatgtata tcgttgcaat tgttgtggta gcaattgtat
     4321 tcgtgttgta tattcgttca atgatccgag ggaaacttgg ggattggatc ttaagtattt
     4381 tggaaaacaa atatgactta aatcacctgg acgcgatgaa attatatcaa tattccatac
     4441 ggaacaatat agatatcttt atttatgtgg cgattgtcat tagtattctt attctatgtc
     4501 gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat aaataccggc attgatgtac
     4561 ttattcagaa cgaagataaa caaattgagc tttctgcgga aatggatgtt atggaacaaa
     4621 agctcaacac attaaaacgg actctggaaa agcgagagca ggatgcaaag ctggccgaac
     4681 aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat taaaacgccc cttacatcca
     4741 ttatcggtta tttgagcctg cttgacgagg ctccagacat gccggtagat caaaaggcaa
     4801 agtatgtgca tatcacgttg gacaaagcgt atcgactcga acagctaatc gacgagtttt
     4861 ttgagattac acggtataac ctacaaacga taacgctaac aaaaacgcac atagacctat
     4921 actatatgct ggtgcagatg accgatgaat tttatcctca gctttccgca catggaaaac
     4981 aggcggttat tcacgccccc gaggatctga ccgtgtccgg cgaccctgat aaactcgcga
     5041 gagtctttaa caacattttg aaaaacgccg ctgcatacag tgaggataac agcatcattg
     5101 acattaccgc gggcctctcc ggggatgtgg tgtcaatcga attcaagaac actggaagca
     5161 tcccaaaaga taagctagct gccatatttg aaaagttcta taggctggac aatgctcgtt
     5221 cttccgatac gggtggcgcg ggacttggat tggcgattgc aaaagaaatt attgttcagc
     5281 atggagggca gatttacgcg gaaagcaatg ataactatac gacgtttagg gtagagcttc
//
LOCUS       DAPBCS010000094         5336 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPBCS010000094 DAPBCS010000000
VERSION     DAPBCS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.098"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..279)
                     /locus_tag="RD521_002752"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="RD521_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="RD521_002753"
     CDS             complement(545..739)
                     /locus_tag="RD521_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772410.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="RD521_002754"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="RD521_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="RD521_002755"
     CDS             2091..3131
                     /locus_tag="RD521_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDT7772411.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="RD521_002756"
     CDS             3955..4287
                     /locus_tag="RD521_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDT7772412.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4903..5244
                     /locus_tag="RD521_002757"
     CDS             4903..5244
                     /locus_tag="RD521_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDT7772413.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          727 c          900 g         1767 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt atgtattttt
     4801 aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa aattaatata
     4861 aagcatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg
     4921 aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga ttacaaagga
     4981 tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc attagacgag
     5041 gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt gctacctgaa
     5101 gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta
     5161 tctgttgaag atacttggtc atatttgagt gattatattg tcagctttga aatcattgaa
     5221 tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag
     5281 aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt aaggaa
//
LOCUS       DAPBCS010000095         5243 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPBCS010000095 DAPBCS010000000
VERSION     DAPBCS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.099"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(3..1151)
                     /gene="nagA"
                     /locus_tag="RD521_002758"
     CDS             complement(3..1151)
                     /gene="nagA"
                     /locus_tag="RD521_002758"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDT7772414.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1227..2063)
                     /locus_tag="RD521_002759"
     CDS             complement(1227..2063)
                     /locus_tag="RD521_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7772415.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2247..3464)
                     /locus_tag="RD521_002760"
     CDS             complement(2247..3464)
                     /locus_tag="RD521_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7772416.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3613..3792)
                     /locus_tag="RD521_002761"
     CDS             complement(3613..3792)
                     /locus_tag="RD521_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772417.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3847..3930
                     /locus_tag="RD521_002762"
     tRNA            3847..3930
                     /locus_tag="RD521_002762"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3881..3883,aa:Leu,seq:gag)
     gene            complement(4170..5168)
                     /locus_tag="RD521_002763"
     CDS             complement(4170..5168)
                     /locus_tag="RD521_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772418.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1623 a         1073 c          831 g         1716 t
ORIGIN      
        1 tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt catatcaggg
       61 ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt atcatcgatt
      121 ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg tgttgctaat
      181 ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc agctaagttg
      241 ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc taaattgtaa
      301 tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac atgatcatgt
      361 cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc atcacaaatc
      421 aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg ttcgcgatga
      481 ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc ttcaacagct
      541 ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac tccttcttca
      601 gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc aatttttttg
      661 attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg atccccgaaa
      721 taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc aaaatagatt
      781 cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc agctacatct
      841 tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac tccacaagat
      901 aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac atcatgattc
      961 atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc gctttggtca
     1021 atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag tccatcggta
     1081 atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc agcatagata
     1141 aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc ttatttagag
     1201 aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt cacagcttgt
     1261 gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta tattaagctt
     1321 ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc ccaatagatt
     1381 ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa gaaatttcca
     1441 taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga ttgatgcagc
     1501 agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc cacaccatat
     1561 atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat tctttctttt
     1621 tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat attttgacgc
     1681 aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt tgcttgatac
     1741 agattttcag aaatatcaga agattttagt acttgttcta acttccgata atcacgtttt
     1801 tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt ttttcgagag
     1861 agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat catcaccaat
     1921 acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga acaaatatca
     1981 tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat gatcgttcca
     2041 aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt cgcattctat
     2101 tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta aataatatac
     2161 tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt ctgtggccct
     2221 cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag gaattctctc
     2281 attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat tcgaaaatga
     2341 tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg ttcatgtaaa
     2401 aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc aatttttggg
     2461 aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc attgattgca
     2521 ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag ctcatcgaca
     2581 ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt ggaacacaaa
     2641 cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga cttatctcca
     2701 cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga tagaccgttc
     2761 agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc tagaccatcc
     2821 atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg tcgagctact
     2881 tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt attaggatta
     2941 atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc aatatcagga
     3001 taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc taatgcaata
     3061 gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc attgtccagc
     3121 aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta gatatcattg
     3181 atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt tctagcagaa
     3241 aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat caaatcacga
     3301 atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt caattttaaa
     3361 atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc gcgaacatcg
     3421 taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta gccaatccct
     3481 catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa tatcatactc
     3541 catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa aacacaaaca
     3601 agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc aatccattgc
     3661 aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg caagtccaat
     3721 caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct ccatcttaat
     3781 aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt agtgtgttat
     3841 tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg tgagcgttag
     3901 ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat tttgcttcac
     3961 gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt atcctttaca
     4021 aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg gaaacaagct
     4081 actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa atccctcacc
     4141 taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat tataaaggta
     4201 caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac actcatgctt
     4261 ttcattagct ctttaactgc cattagtact aagatggcga taacaacata aagtatatag
     4321 tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa ccttgaacaa
     4381 aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc aaaacttaaa
     4441 taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc tatccaagta
     4501 cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt caggttacta
     4561 ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg acccattgta
     4621 ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat acgcacataa
     4681 tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa taaaaataag
     4741 atattcatat ctaaaaaaaa cggctttcca aatgatgaaa tacctaaatc taacttatta
     4801 agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc agcacgtaat
     4861 gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat tgaaaatact
     4921 gataacttat aattatatcg tccattgaca atcaaatata aactaactat acttgttaga
     4981 acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa aggaatactt
     5041 acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa cggataaaaa
     5101 gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct ccaaccggtt
     5161 tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa taaattttta
     5221 acatttattt tctcaaaaac ata
//
LOCUS       DAPBCS010000096         5179 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPBCS010000096 DAPBCS010000000
VERSION     DAPBCS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.100"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(139..1101)
                     /locus_tag="RD521_002764"
     CDS             complement(139..1101)
                     /locus_tag="RD521_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDT7772419.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1103..2542)
                     /locus_tag="RD521_002765"
     CDS             complement(1103..2542)
                     /locus_tag="RD521_002765"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDT7772420.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2727..4673
                     /locus_tag="RD521_002766"
     CDS             2727..4673
                     /locus_tag="RD521_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDT7772421.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4937..>5179
                     /locus_tag="RD521_002767"
     CDS             4937..>5179
                     /locus_tag="RD521_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7772422.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1669 a          878 c          855 g         1777 t
ORIGIN      
        1 ggatttctcc actccaaaaa agtctaactt ttgggggtca ctacaagttg acaactcctt
       61 tttgtttaca aatcaattgg atatctacaa attgtatttt ctaacgaatt ttatagacat
      121 taaaaaatta atttaacgtt aaatacttcc tccagaaagt attgagattg gtaaaatata
      181 atccttattt gtcttttcgc ctttaatttt tttcagaaga atatcaacaa taagacttgc
      241 aatttcatca attggttgtc ttattgttgt caattgtggg aaaaatttct caataaaaga
      301 ggtaccatcg tatccaataa tttttaaatc ttctgggata ttaagtttaa gttgatgagc
      361 aattttcata gtgagaatgg ctgttaaatc atcagaaaca aaaataccat ctggtttgtg
      421 atttataata gttgacttta tttccatttc acgacggata gtagacagat tattaggaat
      481 tttgaaaatt tttccatttg gattttgaaa agaaaaacct agtgcacgta attctgtggg
      541 agaatccgtg ttatcatttc ctgtaatcat aatggtattt tgacaaccat ttttttgtaa
      601 agtcttagct gcaagttttc ccccttcaaa gttatctgaa gaaacaatgg aaacattagg
      661 agctaaattt ctatcaaaag caacaatagg tgctctcaca cgctcataat cagcaattcc
      721 aagattatgg cttgatgaga ttatgccatc aacttggttg gcttcaagca tttcaatgta
      781 ttctctctct ttagcaggat cattttgact attgcaaata atcgttttat atccatgttt
      841 aaataactct atttcgagat actcaatgag ttcagagtaa aagatattac taatattagg
      901 aaaaataaga ccaataagct gaggtgactt tccttgaagt ccacgtgcta aattatttgg
      961 tttataacca agagttttca tagcttcatg aactttttgt tttgtttttt ctgataaata
     1021 tcccttatta ttaatgacgc gtgaaacggt tgttgggcta actccagcta attcagcgac
     1081 atcagttaat tttgctacca ttttaatatt ttaattcaaa gtattttcca gtaggttcgc
     1141 ctgatttgat ttgaatacca ttttggtgat tttcagggaa tacacgacta gtaaacactt
     1201 tttctccttt attaataaaa atctcaatga ttgagttatc tacaaaaatg ttagcagttg
     1261 tttctttagt atcaattgag cattcacgaa tcgtaccaaa gtcagtggca tattgaatgc
     1321 ctgctttgct gcgatctaaa ataatttttc cttctttggt atctacaatc aagctaagtc
     1381 catttccttt actatcagag aaaagaagta actccgattt ctgatttgga ggaaaagtta
     1441 gttcaagctc ataagtatta tttgtttctg cttttgctgt aaattgttca gaactagcac
     1501 gtaatgatgt aatagcttct actggatatt ggtaaagttt accatctttt attgaaagtt
     1561 cttttacaag actcattgct ccctgataat catacttatc agttggataa tcgatatcag
     1621 gtagtccaat ccaactgaca atcagagcac gaccatcagg agtattgaat ccttgagtcg
     1681 catacgcttc aaatccataa tcaagatttt gaatttctga agcgcccaca agttttactt
     1741 tttctatgtc aagcgattga cagatcttaa aagtattcgg atagatattc ccataattta
     1801 gctcagattt atccaaccct tgaggacaat aaatgagaac aggttttttg tctacaaata
     1861 ctagatttgg acactcaatc atgtattctg aaccagtgcc gccaaaatca aggttgccaa
     1921 cttcctccca tgtttccacg ttattgtcat ccgctttgta aagtttgata aagcctgatt
     1981 tttctaaatt ttgtgctcca acaatcgcat acaattgtcc ttcataatta aacatttgtg
     2041 gatcacgaaa atgatcagtt acatcagcag gtttatgaat aagaacattc tcacctttcg
     2101 taatgttgta gtctttatcc atccatgctc cgatttgaag tgtatcacgt tcccaatttt
     2161 catttcgaac gtttcctgta tagagtaaga atagattccc ctctatttca tatgcagaac
     2221 cggaataagc gccgtggcta tctcgttttg tgtctggaag aagttctgct cctgttacat
     2281 gaaaatgaac taaatcatct gattcattgt gtacccattc tttcaaccca tgagcagctc
     2341 caaatggcca attttgataa aaaagcgtat attttccgtt aaaaaaagaa aaaccattag
     2401 gatcgtttaa caatcctgtt ttaggttcga tatgatagct agcacgccaa ggtgatcgtt
     2461 ttacattatc cttaatttta tccatctcat ctttagtcca atcagaataa gcacgatatc
     2521 gtatttctcg aggtaagttc atattttact ccttcaattt atctttttac aactagtatg
     2581 gtaaacgttt tccgttgaaa aatcaatcat ttcatttaaa aaaataaact atatgacaaa
     2641 cgtttgacat atttttaaga tatgctaaaa taaatttgca tttcagtgaa ccgttttcac
     2701 gtaaaaataa aaaaggagtg attcgaatga attacagtaa agtagcttcc gacgtaatac
     2761 aagcggtcgg aaaagataac cttattgcag ctgcacactg cgctacacgt cttcgtctcg
     2821 tactaaaaga tgacacgaaa gtgaatcaaa aagctttaga cgaaaatcca gatgtgaaaa
     2881 gcacatttaa aattaatgga caatatcaag tgattatcgg tgcaggtgat gttaatttcg
     2941 tttatgacga acttatcaaa aaaactggac tttcggaaat ttcaacagat gatttaaaac
     3001 aaattgctag taaagataaa aaattcaacc ccattatggc attaattaaa ttattatcag
     3061 atatttttgt tccaattata cctgcccttg ttgcaggtgg tttattaatg gcattacaaa
     3121 actttttaac agctgctgga ctttttggac caaaatcaat tgaagaaatg tatcctgcga
     3181 ttgaaggtat ttcctcaatg atccaactga tgtccgctgc accattcatg ttcttaccaa
     3241 ttttagtagg tatttcagct gctaaacgtt ttggagcaaa ccaatttctt ggtgctgcaa
     3301 ttggtatgat catgacaaca ccagatcttg gtggctcaac tgaatattgg aatatttttg
     3361 gttatcatgt ttcacaaaca aactatgctt atcaagttat tccagtttta gcagctgttt
     3421 ggatattatc tatattagaa aaattctttc ataaaaaatt gccatcatct attgacttta
     3481 cattcacgcc attattatca gtaatgatta ctggattttt gactttcaca gttattggcc
     3541 ctgtaatgtt attgttgtct aatggaatta cagacattat tgtttggcta tataacacta
     3601 ctggatttat tggaatgggt atttttggag gaacatattc acttatagtc atgactggtt
     3661 tacatcaatc atttccagct attgaaactc aattactttc agcgtggaca aatggtatcg
     3721 gacatggaga ctttatcttt gtagttgcat ctatggcaaa cgttgctcaa ggtgctgcaa
     3781 catttgccat ttggttctta actaaaaatt ctaagacaaa aagtcttgct tcctctgcgg
     3841 gtgtctcagc acttcttgga attacagagc ctgccttgtt cggagttaac ttaaaatacc
     3901 gctttccatt cttttgtgct ttaattggtt caggaattgc tacagcagta gcaggattac
     3961 ttaaagtagt tgctgtttca cttggttcag caggtttctt gggatttctt tcaattaatg
     4021 caacatctat tccattttac gtattgtgtg aattgattag ctttgtagtt gcttttgcaa
     4081 ttacttactt ctacggtaaa acaaaagctg ctggtatctt taaagcagaa gaaattgctg
     4141 aacaaacacc agttaattct tcaaaagtag caacaacaga tccaatatct actgatgttg
     4201 aatcaacaca aacaaaagtt tcatcagaaa caattgttag tcccatttct ggtaaagtga
     4261 ttagtctttc ttcagtaaat gatccagtct tttcatcaga agctatggga aaagggattg
     4321 ccattaaacc aaatggaaac acggtccatt ctccagtaga tggtactatt caactcacgt
     4381 ttgaaactgg acatgcttat ggtattaaat caaataatgg tgctgaagtt ctaattcata
     4441 ttggtattga tactgtatca atgggaggaa aaggatttac acaaaaagtt actactaatc
     4501 aaaaagttag aaaaggcgat attcttggag catttgaccg ttcaatgatt gagaaagaag
     4561 gtcttgatga tacaacaatg attattgtga ccaatacttc ggattattca gaagtaaata
     4621 tagtagctga aaatacagtc actgagggta ctgatttact tgaaatcaaa taaatatatt
     4681 tccctgagta aagatggaat tttctttcca aaatcaaata atatagatta atcaaatatt
     4741 tgagaataac attttattta atcttttcat atatcaattg tatgaaaaat tattagaatt
     4801 ttaaaaaatg ataaaaattc aaaaagaaaa aatagtagtt atttacttgt catgttacta
     4861 aataaaaaaa taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat
     4921 aaagggagaa atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg
     4981 tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc
     5041 tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg
     5101 gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta
     5161 cattacctcg accccgaaa
//
LOCUS       DAPBCS010000097         5169 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPBCS010000097 DAPBCS010000000
VERSION     DAPBCS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.101"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            919..1887
                     /locus_tag="RD521_002768"
     CDS             919..1887
                     /locus_tag="RD521_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7772423.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1905..2429
                     /locus_tag="RD521_002769"
     CDS             1905..2429
                     /locus_tag="RD521_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDT7772424.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVVEKTKTENHKNANQVPQLEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTILSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            2805..3086
                     /locus_tag="RD521_002770"
     CDS             2805..3086
                     /locus_tag="RD521_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772425.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIDIQTKNTETLNDYVHTNNQIIETLIGGI"
     gene            3088..3504
                     /locus_tag="RD521_002771"
     CDS             3088..3504
                     /locus_tag="RD521_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772426.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
     gene            complement(3952..4971)
                     /locus_tag="RD521_002772"
     CDS             complement(3952..4971)
                     /locus_tag="RD521_002772"
                     /inference="COORDINATES: protein motif:HMM:NF019371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDT7772427.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQE
                     MIKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSF
                     IRNQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGH
                     FNSFFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCY
                     DNPKYKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQ
                     KLYLYGGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKL
                     KIMIV"
BASE COUNT         1889 a          862 c          802 g         1616 t
ORIGIN      
        1 ccccctgtta gaatgcgggg ggctaaatgc gatcggtcta tcgagcttgc tcgatagacc
       61 tttttttgct tgaaaatggc gcaaaaccgg tgcctttcac gaatttgtaa gcttgtttgt
      121 acacgtctcg ctctaggaga ccttgttcgc tccgcaccgc aagcagcttt tagcaacagc
      181 acttttttgc taacgtacga aaatggctct tccggaattc tgctgaatcg gttgcttatt
      241 gttttgtttc atacgctctt atttgcgttt tcagacgttt tactacccaa ggtatataaa
      301 atatacaaga acatagataa gacagattaa atcgaatttt aggatggttt tatgctcttt
      361 aagggttgtt agggcttgcc ctgaccgcct gtaaggtgct ttaagaacaa gataagggtt
      421 tatccatact aaactgtctt aaaacggctt aaacgagcgc gcagagcctc taaaaaaatc
      481 tctttaaaca acgtttttag caaaggagcc tttaggcgac tgcgcaggta caatgtgagg
      541 gtgtttttcc ctcacacctt tttagcgacc agagggagca agtttcctag tttagtaaaa
      601 ttcgcgaacc tgtttggcta aaaaagcaga aaatttttag gcgggaaatt ttgcccctct
      661 cctcttttat aatctctttt taaacctctt tttaaacctc tttaccgtcg gctgtatgct
      721 tactctccca aggtgtgcag aatcttttgg gggtcttttt gtctgttttt aggggtcttt
      781 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttaggg
      841 gtctttaatt aggggcttga cccctattag aaagctcttt ataattttgt taagtagtta
      901 atcttgaagg aggattttat ggcaaatgaa attgtgaaat accaccatga attaaatacg
      961 attcctttac gcaaatttac gtcagtagaa atgaatttat tcttctctat agtatctaga
     1021 atgcgtgacg taggcgataa aaaagtacaa tttacattcg aacaattaaa agatttaagc
     1081 aattataaag caactgcaaa tgttcgattc atagatgatt tagaaacaac gtatgacaaa
     1141 ttaatggatt tacgttttgg tagaagaagt gctgacggtt tgcaacgtga acgatttgtt
     1201 ttatttaatc aatttaaaat cgatggaaaa gctgatattc cttttgctga aatacaagtc
     1261 catgagaaag ccttaccctt acttaataat cttgaagaat gggttcgata ttctctccaa
     1321 caattcaatg aattagaaag cagttattcc aaaaccatgt ttagattatt aaaacaatac
     1381 agaaccaaag gaattgccta tttttcaaaa gaagactttc atgaattact tgatatacct
     1441 aaaagttata gagaaagtga tataaataaa aaaattataa agcctattcg ccaagaacta
     1501 accgcaatct tcaaaggtct gacaatccaa aagagatacg gaaaaggacg gggaaagcct
     1561 gtcattggct atcaatttac atttaaacct gagataaaaa aatcagacga ctttaacaaa
     1621 catgccccaa caaaagaaga acaaaacaaa gcattagaac tagctaacca acaagttcaa
     1681 tctgaaaaag cacctacttc attacaagaa aaaatcaaag aacaaaagcg aaaattggaa
     1741 cttttacaac agttagaaaa gttagaaaga gaacaaaata cccaaaataa agaaaataat
     1801 gcacaaaaat cagagataaa ttccgatatt ccttcagaac taaaaaaaga cttattagac
     1861 aatcttcaaa acttattcaa agattgaggg ggtttttaga ttttatgaca gaaaaaacaa
     1921 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     1981 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     2041 gacaaaaggc tataaaatca atgtttttta acgaaaatga aaactcaaat caacaactag
     2101 ttgtcgaaaa aacgaaaacc gaaaaccaca aaaacgccaa ccaagttccc caattagaag
     2161 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     2221 agcaaataaa acaaatgcaa aaattactcg accaacaaca acaactcacc ctgcaagcca
     2281 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     2341 aaaataaatc aacgattctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     2401 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaatt taaattgctg
     2461 gttttattct tctttttgct tttgcgagca gagcaacatg ggcccatgtt gcgatatttt
     2521 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     2581 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     2641 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgcc
     2701 attatggata gcttatgtat agtgcttcgg caaaaaacag ctatacaaat atagtatttg
     2761 tatgatatac ataaattata cataattgca aaaggagatt ttttatggac attttagttc
     2821 ggaatattga tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata
     2881 aaacaggaga aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     2941 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     3001 atattcaaac aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaaatca
     3061 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     3121 ctttatccca gtcacattga cgttttagat tccataatcg aaaaaaatcc agatatcaat
     3181 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     3241 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     3301 atcattgcag aatttgctga tttgtattta aaagatacag gcgttcttat cggtacaaac
     3361 tcgaaagtat atcaagaagt aaaagaaaaa attgaaaaac agctaaatcc ctcccaacga
     3421 aatcaaccaa agaatcgtca aaaagaaacg agtcaaacat tcacttctaa aaatttcgac
     3481 ccaaccaact ttctaaaaag ataaaaaaat aaaccaggaa atttttaatt tcctggttta
     3541 ttttctagag agcggtcttc caataagatt tgcaaatcga tcccaatttg cgtaaaaaaa
     3601 aaaagctgct cgtacgagca gctaaacaag aaaaagttag cttgttttaa gggtaaaata
     3661 agtgtaacaa attttgcgaa tgtaacgatc ttttttatta tacgcaaaca atcaaaaaaa
     3721 aagactgctc gaaagagcag tctcacaagc gctcacaaaa ataacaaccc cctaaaaaca
     3781 gaccggtcca aattcacaaa gaaatggaag aaacaaatcg tatcaaatca agctttagaa
     3841 agtctctttt agctagacca atgaaaaatc acaaagtgag cgtctctatc cattggtaga
     3901 ggggctactt ggcatactcg acattatgtt aactaacaat ttaaataaca attaaacaat
     3961 cattattttt aacttgtttc tccaattttc cggaaaacca taattggatt tttctaagga
     4021 agggatttta tcgatctttt tttctaaatc tattagaaat ttattccata aagtttgtgt
     4081 catgctatct tcatatatta acagatgttt tataacgtat aatcctccat ataaatataa
     4141 cttttgggtt tcttttaagt tattttcagg aaaataactt ttggaatcat tttttatgat
     4201 tgttggagcg tcagtaactt taaatgcata tattcttgaa tgatgagcta tcttatttct
     4261 ataagctcta attacgtttg cccaacttcg tatagctttt ttgtatttag gattatcata
     4321 acagctcatg acccaattat tataatactc tgtattaagt tgattaataa atgttgttaa
     4381 actaccaaat gttatttctt caaataaagc ccatattggt aaacaatgat ttttttttat
     4441 atgatgatgt ttaaaagtgg gactttgatt tgaataaaaa aaactattaa aatgaccaat
     4501 tatatcttca gcccatttca aatctgaata aatattttta tccaaataac actgattctt
     4561 cggaaatatg ctagtttctt cataagaaac gcacatatta tctgaaagag attttttcca
     4621 aaaaatttct atttgattca caaaataatg caattgatta cgaatgaatg aatctaattt
     4681 gtacgtttca ataacatcta gaatagtaac tttatctata tttttcatta atttaacata
     4741 aatactaaat cgaaaataac tatttttctt taaaaacttt tctaataaaa ttgtatcgga
     4801 atttgttaaa aaaatatctt tacttatttt ttttatcatt tcttgctcgg ataaatatgg
     4861 ttttaactct tgattaatta ctttggaatt attaattaca cacaatgcct ttttaggtgg
     4921 aattctatga ttaactgttt taactttata ttttctattt gatgtcttca taagaatctt
     4981 ctttcttaaa aaaatataaa aaaaaagccc taccataatg cgcatgatat taaatcagag
     5041 gcgtggcaag tactgataga tatattaaaa cacatttgaa ttaaactgta aagctttttt
     5101 atttaaaaat ttagttgtca aaatcgtgat tttttttaag tgtgcaacca aaagaaatgc
     5161 ctatatatc
//
LOCUS       DAPBCS010000098         4525 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPBCS010000098 DAPBCS010000000
VERSION     DAPBCS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.102"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..218)
                     /locus_tag="RD521_002773"
     CDS             complement(<1..218)
                     /locus_tag="RD521_002773"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7772428.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKP"
     gene            complement(329..583)
                     /locus_tag="RD521_002774"
     CDS             complement(329..583)
                     /locus_tag="RD521_002774"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7772429.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(776..1051)
                     /locus_tag="RD521_002775"
     CDS             complement(776..1051)
                     /locus_tag="RD521_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDT7772430.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1023..1976)
                     /locus_tag="RD521_002776"
     CDS             complement(1023..1976)
                     /locus_tag="RD521_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7772431.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2588..4081
                     /locus_tag="RD521_002777"
     CDS             2588..4081
                     /locus_tag="RD521_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDT7772432.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4216..4512
                     /locus_tag="RD521_002778"
     CDS             4216..4512
                     /locus_tag="RD521_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDT7772433.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT         1478 a          752 c          792 g         1503 t
ORIGIN      
        1 atcggtttat tagacctttg aggtctatat tttttaacta caatcgacct taaatttaat
       61 tgtttcatta gcttttggac atgtttaaga gatactgaaa tcccttcttt taataaaact
      121 tgatggatct ttgtggcacc atagcgttgt ttattcgtaa aaaaaattgt tgaaatcttc
      181 tttttcaact tattatttct gatttctgta gctgtcaacg gtttattctt ataaaaataa
      241 tagacacttc tagggatttc taataaccga cacaacaaag aaacagtgta atccttacac
      301 catttttcaa taaattgaac aagcgaattt aggttttccg agagaatatg gctgcggctt
      361 tttttaagat ttcaagttcc tcatttaaac gagcattttc ttttctcagt tcagctactt
      421 cttttccagt cagtcctgtg gactgatcag gtaaatataa attcttccat ttgtaaatcg
      481 ttgcttcaga taagccatat tctttcgcta accctcgaac agaacgtcca gtttgattca
      541 gttcaacaat catttgttta aaattttctt catatcgggt cataaaaatt catcctctcg
      601 tagataagtt taatttattc ttgtctctac gaaaagctgt ctaagatttg atactaacac
      661 caaatgttgt atacccccaa acaatacaca tattttgaag gattaggatt aataaagcta
      721 aaaatatagt aagtatacta aatatattta ctatatctag tatacttact atattttaat
      781 cttttgatcg tctattatat ataatccgtt cctcgtcgtt taaagtatca acataggcgt
      841 ccattgcaag tttgactatt tcatacatag gaatatcttt tgcataacta agatcccgaa
      901 tggtttttag tacgggagga tctacttgaa ctggttttct ttctttcgca gtgtactttc
      961 gttcccctaa ctcgacatta atatcatctt taattgaaat atcttcagca ccaattttct
     1021 tcttatcttt gttcgttggt aataatgcca tttagtttaa tctcctttcc aagaggtagg
     1081 atatgattgg ccattgagtc agcatattta ggtgtataat caaatccttc aatatcacca
     1141 tgttgttcat agtattcaat tctttcttct aattctgtaa agatgtctgc aaaaatagcc
     1201 caccatcttc gatctacata atcatttaat tggataccat tctcaccata catttctaac
     1261 cgatccgagc ctttaacaat gcttttaaaa atgtttgatt caccatactt atcaattgtt
     1321 ttttgatagt ttgcgtgttg agaggaacgt cgcactgaga aaagaacagg caaaattcca
     1381 atgatttgaa gatgagactc ttcaaaattc gtgacaatcg gaactaaaac tttattaata
     1441 aaatcttctg ttccttccat tgcgtaccgt tttaattctt gcattgcaat aatataatct
     1501 gcagcagtta agaatgcacg aaccacatta tctgttgatg gtgggcagtc gataaagata
     1561 taatcgaaat tttttctcaa ttcttctaaa ggtttaatga aggccaatga tctcttacga
     1621 cgatcaggat atttttcgga caagtattca ttaagttctt cagatccagg agagccagcc
     1681 ataatatata aatgtggact tacttgagta atacctgaaa cccagtcccc ttttttgatt
     1741 gctgcagaca tggatcgatc aaagctagta atttgatacg ttgatccaat gttggaagta
     1801 gtatttcttt gttggtcata atcaattagc aacacgtctt tttcaaaaag ttgactagaa
     1861 acaatcgcta ataaatcatt gtctgtagtc ttaccaatgc cgccttttct acctgtaagc
     1921 attacaactt ttcctagttt atctgatgtt ttaaatgcag tccgtaagtt ttccatagtt
     1981 ccctccaatt tcctatacta aatatattta gtatacttca tatacttaat atattcgata
     2041 tatctaatat actccaaatt gataagaatt cagactttat aaggaatata gtggatatac
     2101 taaatatatt tgctatattc agtatatcag atatattcaa aatgtcaaaa tatatttgtg
     2161 cgttttgcgt tgacaattta gagtgaaaag aggtaaactt aattctatag aaaccacaga
     2221 taattcacta tttacaaagt agcaaaagca gtggtactct ttaaacagaa aaattacata
     2281 aaaaaaagag acggaaataa ttgattttag cagaatcaat tatttctgaa tcacatggtt
     2341 aggacatgtg ttcacgctcg tctcaagaat atgtaagtag ctaccaacta cttatattaa
     2401 aagttgatcg gatacctttt ccgattgcct ttattctacc aaaaaaaggc gagcaactca
     2461 acagaagaac gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac
     2521 acatactatg gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt
     2581 acaatcgatg ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa
     2641 atatcgaaac agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat
     2701 ctttgcgtac cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga
     2761 agaagctatt ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt
     2821 tggtacttat gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag
     2881 tcaaatcaac acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat
     2941 tttagcctgt ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg
     3001 ttatcaagtt tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa
     3061 ggtgatcgat gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt
     3121 gtccggagtg gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa
     3181 tattgttttt aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat
     3241 gaagcttgaa tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc
     3301 tgagaaaaaa gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc
     3361 aaacatcgtc ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt
     3421 agcttattac tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa
     3481 cgaacggtta caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta
     3541 ttctggtaat tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt
     3601 ttcaccaacc ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa
     3661 aaagccacga gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc
     3721 ttatctaagc gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt
     3781 ggcttcacaa ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga
     3841 ggagaataaa gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc
     3901 agtcaaaacg ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc
     3961 aacaaggatc acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca
     4021 attatcaacc cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg
     4081 aaaaataaaa ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc
     4141 tccctttgcc tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat
     4201 catggaggaa gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt
     4261 atcattggtc gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag
     4321 gggactttgt cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt
     4381 cttcatgggt tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt
     4441 gtgttagagc aggagtatat tgagtataaa atagctaacg agttaagaaa aaggaagaag
     4501 tatatccagt agtagttttt attta
//
LOCUS       DAPBCS010000099         4425 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPBCS010000099 DAPBCS010000000
VERSION     DAPBCS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.103"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="RD521_002779"
     CDS             690..1658
                     /locus_tag="RD521_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDT7772434.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="RD521_002780"
     CDS             1669..2484
                     /locus_tag="RD521_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDT7772435.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="RD521_002781"
     CDS             2552..3262
                     /locus_tag="RD521_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDT7772436.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="RD521_002782"
     CDS             3298..>4425
                     /locus_tag="RD521_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDT7772437.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAPBCS010000100         4416 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPBCS010000100 DAPBCS010000000
VERSION     DAPBCS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.104"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            59..913
                     /locus_tag="RD521_002783"
     CDS             59..913
                     /locus_tag="RD521_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDT7772438.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="RD521_002784"
     CDS             1010..3259
                     /locus_tag="RD521_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDT7772439.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="RD521_002785"
     CDS             3262..>4416
                     /locus_tag="RD521_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7772440.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          806 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DAPBCS010000101         4385 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPBCS010000101 DAPBCS010000000
VERSION     DAPBCS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.105"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..1051)
                     /locus_tag="RD521_002786"
     CDS             complement(83..1051)
                     /locus_tag="RD521_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDT7772441.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1500..2915)
                     /locus_tag="RD521_002787"
     CDS             complement(1500..2915)
                     /locus_tag="RD521_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7772442.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLTSFEKLPDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDLSA
                     VVAENKKLKEENRSLRQRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="RD521_002788"
     CDS             complement(3137..3499)
                     /locus_tag="RD521_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772443.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3503..3841)
                     /locus_tag="RD521_002789"
     CDS             complement(3503..3841)
                     /locus_tag="RD521_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772444.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3820..4239)
                     /locus_tag="RD521_002790"
     CDS             complement(3820..4239)
                     /locus_tag="RD521_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772445.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4232..4372)
                     /locus_tag="RD521_002791"
     CDS             complement(4232..4372)
                     /locus_tag="RD521_002791"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772446.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1247 a          970 c          613 g         1555 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgct gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacaaat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgatacga gccatccggt aatttttcaa aactcgttaa aggaacaggg
     1981 tctttttgaa aaacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaacg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat gtgcttatgc
     2821 caatcattgg ttttctggcg atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gattgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg atgtcacaat tccaaacgct ctccgagggt tttcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtatcacgc tatcaactcc ttttatgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct
     3181 cactcatctg acttttctta ttcggtatca ctccgaataa gtcgtcagat tcatcgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgtttttt
     3301 cgtgttgctg atcgtcagaa attgacgata accaccgctc aaaatcagca aaagtttcaa
     3361 acccaaaacg cttgcgaaga gccttgccca ttgcagcatt ttctttctca atcaagcgtt
     3421 ttttctgttg gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat
     3481 ctgctaaagt cattcccata aactacatct ccttttcttt ggaattaata acggaaattt
     3541 ggtttaactg cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag
     3601 ttgtttgcgc taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctagat
     3661 cagcattgat aaccgagttg agaacatcca taattcgttc aagcatttca ctataattat
     3721 cttcatccgg caaattcaaa taatcagcta accgttcata catcaattca attctatcga
     3781 ctgtttcttc tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca
     3841 ttttcgattt tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag
     3901 caaagtcaat ttttgttggt cacgaatcat ttccgttaat tcagaaatat gcctatcttt
     3961 ttccgctaat tgttcacgca aaaattcatt ttctttttct aacgggaaat cattgcctgc
     4021 atcattgacc ggtaaatcac cagtcaattg accacctttt gaccgataaa ttgaccgcac
     4081 aacaccaatc aattccttgt ctaacatgat aaccccatta tctttatacc gagcaaggtc
     4141 ataacctgca ttgaccagtt gattgcgcac tgtattttta gatataccca actcatcagc
     4201 aaggtttttt atggtcattt tttctggcaa atcagtcatt atcttctccg ttactttcag
     4261 ctaccccata aacatattta acagcatgct tagggataat gattggggaa tcaattttat
     4321 tatttttcaa aatcaactca tactgcgtta catcaagtaa cttaccggac atttttttgc
     4381 tatcc
//
LOCUS       DAPBCS010000102         4371 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPBCS010000102 DAPBCS010000000
VERSION     DAPBCS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.106"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            106..357
                     /locus_tag="RD521_002792"
                     /pseudo
     CDS             106..357
                     /locus_tag="RD521_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="RD521_002793"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="RD521_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDT7772447.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="RD521_002794"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="RD521_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="RD521_002795"
     CDS             1786..1869
                     /locus_tag="RD521_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDT7772448.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="RD521_002796"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="RD521_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDT7772449.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="RD521_002797"
     CDS             complement(2994..3857)
                     /locus_tag="RD521_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HDT7772450.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="RD521_002798"
     CDS             complement(3859..4131)
                     /locus_tag="RD521_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDT7772451.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="RD521_002799"
     CDS             complement(4148..4357)
                     /locus_tag="RD521_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDT7772452.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT         1352 a          735 c          822 g         1462 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc t
//
LOCUS       DAPBCS010000103         4281 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPBCS010000103 DAPBCS010000000
VERSION     DAPBCS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.107"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(37..1257)
                     /locus_tag="RD521_002800"
     CDS             complement(37..1257)
                     /locus_tag="RD521_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDT7772453.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="RD521_002801"
     CDS             complement(1277..1888)
                     /locus_tag="RD521_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDT7772454.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="RD521_002802"
     CDS             complement(1931..2863)
                     /locus_tag="RD521_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDT7772455.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3283..4143)
                     /locus_tag="RD521_002803"
     CDS             complement(3283..4143)
                     /locus_tag="RD521_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7772456.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1218 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa acaaataagg gaaacaaaca acacattttt
     3061 gtgtgttgct tatttccctc taactatcgt tttctatttg atggttaacc tacttaaaat
     3121 aaatactgca acgacaaacg gagatcaagc atgctttgta acgtatttta cgataattac
     3181 gactttattt tattactcta tcttgctgcc atacccgtct atccctaatc atctttggtg
     3241 aattttttca aagttagcat aacaaaaata agtatttttt ccttagttaa tcaatagtat
     3301 catttttttc atatgattta attcatcaat taattttttg tttttattaa ttttagcaaa
     3361 agctagtgca tcttgaatca attcgagcac ttctgccttc gacttatttt tagcctgtgc
     3421 gttcttcgct aattgggcag cagctcgtgc taagtaataa gtcaaatggt tatccgaaca
     3481 aatatcgatt gcgtaattta gtagtgcatc acttgtttcc aaatcaccgt cttgcgcaaa
     3541 aaaaacacca caatgaaaga cgatatttaa cacccgccag atatcttctg ttgtacgaat
     3601 cggatacgta taaatttttt caaacactcg attgaaataa acttctgctt tttccaactc
     3661 tttttctcta gcatagacca ttccaatacc tgtatatgcc aataaacgaa acaacgtgtt
     3721 ggctgtttct gccatcaaca actgatcaaa atggaacaac acatccgtaa ttggtgcatt
     3781 cgtaaaaatc atttcaaatc cttttagata gtagtaacgt gcaatcagct cagactcgtc
     3841 aattaccttg ggatcaatgg ctgctaaaat ctgtgtcaat tgttcatatt cactcgtaat
     3901 cagtgaaaac tctgcttttt ctagctgttc atcaagttgt gtattgatca cgttcatcga
     3961 agaaaaaagt tcaactaacg gaagattgat tcgattacat aatttgatta aaatttttaa
     4021 cgaagggact tgaccattgt tctcaaattt acttaatgtt gcttgtgtac aaattccttc
     4081 agacacttct ttttgagata agttggcagc ctttcttgcc gtaataaatt tttcaatgtt
     4141 cattattttc tctccaatca gacctcacac gaaaatcatt tctgtctcga ttttagcatg
     4201 ttttctttgg gaatgcttta ggttctgttg caaagtttta aataaagaat aaagtccctt
     4261 acggtatcta tgatttaagc t
//
LOCUS       DAPBCS010000104         4079 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPBCS010000104 DAPBCS010000000
VERSION     DAPBCS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.108"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..153)
                     /locus_tag="RD521_002804"
     CDS             complement(<1..153)
                     /locus_tag="RD521_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7772457.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="RD521_002805"
     CDS             complement(475..696)
                     /locus_tag="RD521_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772458.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="RD521_002806"
     CDS             complement(794..1039)
                     /locus_tag="RD521_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772459.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="RD521_002807"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="RD521_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2250..3251)
                     /locus_tag="RD521_002808"
     CDS             complement(2250..3251)
                     /locus_tag="RD521_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDT7772460.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3410..3676)
                     /locus_tag="RD521_002809"
     CDS             complement(3410..3676)
                     /locus_tag="RD521_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7772461.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3666..4022)
                     /locus_tag="RD521_002810"
     CDS             complement(3666..4022)
                     /locus_tag="RD521_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7772462.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1290 a          743 c          540 g         1506 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aaggcattta acattgacat atcttatgta aactaataag atttgaattt tattttatac
     2101 acactcgata atagatcaat tttatggtcc cccgcattta cgattgcaca agagaaacat
     2161 aaagtcgacc tattagcatt tcttttcaat tcaaacatta aaaaagttta tacctctgct
     2221 actaaaaaat gaaattatct atttaaaatt taaaacggta aatctgattc atccaaattg
     2281 gaaaatggat cagcctgtat tggagctttg tatttctcca taataacaga tatctttttt
     2341 tcatttttta aaaaatacct tttaaatttc ttatagtcct ctgaaatcaa aatatagcgt
     2401 gatggatact catcaaaaga agaatcgtat tcttccttta aaattcccga tttacttaat
     2461 ttttctagta catcaaatac tctatcactc aagtaactcc ttaaattgtt atcactttcc
     2521 cattgaacta cttttctata agttgaatta tcagttgaaa agatagaatt ttcatccata
     2581 aacgcatata ttagcagcaa tctttcatca ttgtcaaatt ctattggatc aaaaatatct
     2641 aagttgtcat caaaatcttt atcagttccg gtaacttttt tgttaattac tgaaggtgtc
     2701 ttgtaagaaa tagaatctaa atcaaagcgt tttgattcta aattattttg tgctttctca
     2761 attaaataat tcactttttt tgtgagaaac cttctgatat tgtctttgct atatgttcca
     2821 taaaaaccct tatcttctaa agagtccctg tacttcttaa cttctatata ctcttcagcc
     2881 aaaacttgtg gaactggttc atctaagaaa aaaaccccta cctcgccttt gctattttta
     2941 taaaagtaat caatttctgc tatagttccc gatctataat tatcaactgg agaacccaac
     3001 ttatggccaa aaatcgctaa taatatatca ctattttcta caatttgttt attaataaca
     3061 ctttgcccag agctattatt tatataagta ggaatcgcat tcgtttccca tcgaattggc
     3121 atcaaaatta cttttgtatt atctgtattt tctatattcc atctatatat ggtttcttct
     3181 acttcattcc tctttgctgc tatatcacct ggtgatgcta taaatacttt tattagattt
     3241 atggttttca tttattgtac ctttctcttt atgtctcttt aaagtaattt ttgtataaaa
     3301 tttattgact tttacactat aggacaactt ttatctattt agtacaaaaa ggcccactaa
     3361 actaattgat tagttaggag cctattacag gtatattttc aactcgcttt taagcaaatt
     3421 ctactaccag ctttttgcct aatgccctag cgatatcatc aagtttatcg aaagatacgt
     3481 tatcccctct ctcaattcga gcaatagtag attgagttgt atgtgctttt tgtgcaagct
     3541 ccgcttgagt taggcctgct tcctcccgtg catgataaag agcaaccgca gtttcgagtt
     3601 ttcttccttc acggtcatat gcttctgcaa aatctttgtc tttttgcttt tcttcaatca
     3661 attttttaat catcatttgt gaaccactcc tctctaagtt cttttgcatg tttaagttca
     3721 cttattggtg ttttctccgt ttttttagta aatccatggg taatgacata ccttccattg
     3781 accacatgga aatagatagc tctttggatg tttgatccta ccttacttct taattcgtac
     3841 aaattagtgt ctaatttttt cacccatttc aaccgttgag ctactagcat cccattcttt
     3901 tctgtttctt ctattgtacg taaaagcttc gcactatctt tttttggtaa tgtttccaac
     3961 cattctaaaa attcatcatg accattaggt cttttaaatg tttcaaaaga tattttttcc
     4021 atacccaatt atatcatata ttgattttat attatagtat ataacataaa aattcgaat
//
LOCUS       DAPBCS010000105         4050 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPBCS010000105 DAPBCS010000000
VERSION     DAPBCS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.109"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            117..458
                     /locus_tag="RD521_002811"
     CDS             117..458
                     /locus_tag="RD521_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772463.1"
                     /translation="MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDR
                     WEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVN
                     KGKRYGIEVRS"
     gene            1308..2633
                     /locus_tag="RD521_002812"
     CDS             1308..2633
                     /locus_tag="RD521_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDT7772464.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            2626..2973
                     /locus_tag="RD521_002813"
     CDS             2626..2973
                     /locus_tag="RD521_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772465.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            3288..>4050
                     /locus_tag="RD521_002814"
     CDS             3288..>4050
                     /locus_tag="RD521_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7772466.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYLGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMG"
BASE COUNT         1558 a          528 c          767 g         1197 t
ORIGIN      
        1 taacaatttc ttttgaaaaa agctataatg aaatggaatt caagaaaggt aatgaattga
       61 tcgtaaactt tgcagatttg tgtagcggtg aatgtttaat taatgataaa tctgtaatgt
      121 cttttggtga tagccaatat ataagaaact tcaaaagtaa aattttagca atgaaatcaa
      181 aagaacaaag ccgaggagtc ttttctaagg gcggtcatgg tcgaaaaaga aaattagatc
      241 aagatcgatg ggaagcaata aagaaaagag aaagtaattt tgttaaaact tataataatc
      301 aatgttctgc aaaaatcgtt aaaactgcta ttcgcaataa agctcaaatt attcgtatca
      361 ttgaaccaga taaggcttat ggcgaatggg cttactatca attgaaagaa caactagtaa
      421 acaaaggaaa aagatatggg atagaagtca gaagttagta aattaaacaa tcgttgattt
      481 ggcaacgaaa tagtgaggga aaatccactc actgaagggt aaactccgtc aagttgtgtg
      541 accagacgga aggacgaatg ttgttgaggt tttagaacca tataaaacag catgtttctc
      601 aaatttttag gataagggaa attgctagca gtttgtttta gaaccatata aaacagcatg
      661 tttctcaaat gagtacttta aattaattga tggcgttcct gctttagaac catataaaac
      721 aacatggatt taaaacaaga agtaagtcag ttctcctttt actttagtaa ggaaaaataa
      781 agtattctca ataaaactta aaataagcaa acctttgttt taatagctaa tcgaagtaaa
      841 acccaaagcc atatgaaccc ctgaatcagc gaaagtttat gatgatttgg attactaata
      901 gtttaggaat tagatggtaa atctaaatta aacaatcgtt gatttggcaa cgaaatagtg
      961 agggaaaatc cactcactga agggtaaact ccgtcaagtt atgtgaccag acggaaggac
     1021 gaatgttgtt gaggttttag aaccatataa aacagcatag ttctcaaatt gtttaaaatg
     1081 attgtcaagt taaaaaaatc tgggatcata taaaatagta tagttctcaa atatgattag
     1141 aaaccctatt aaagagcaat attaagccaa cttacttaaa tcgtttaatt aagaacaata
     1201 atctctttta gtcagacttt tagtctgact tttttgtatt tccaaaattt attctttaca
     1261 aaaacgaaca tacgtttgta taattgcttg tgaatggggg aataaaaatg tttgattaca
     1321 gtagagaacc ggtgaatgac tattttttaa tcgacatgaa gtctttctat gctagcgtgg
     1381 aatgtattga acgaaattta gacccaatga ctacaccgtt aattgtcatg agtagatcgg
     1441 acaacactgg ttcaggtctg actctagctt cttcacctac tgcaaagaaa aaatatcata
     1501 ttactaatgt tagtagagct actgatttac cttatccatt accaaaagaa cttcatattg
     1561 ttccaccacg aatgaactta tatataaaaa aaaatatgga agttaatcag atttttagaa
     1621 agtatgtcgc agatgaagat ttattgattt attcgattga tgaatcaatt ttaaaagtga
     1681 caaaatcctt aaatttattt actagttcag gaacaagaag cgagagaaga agagaattag
     1741 ctaaaataat caaagatgag attcagaaag aaacaggtct aatagcaaca gtcggaatag
     1801 gagataaccc attattagct aaacttgcac tggacaatga agcgaagagt agtaaagaat
     1861 ttatagctga gtggacttat gagactgtac ctgaaaaaat atggaaaata aacgagatga
     1921 ctgacttttg gggcattgga tcacgcatga aaaaaagatt gaatcatatg ggaattatga
     1981 gtatagaaga tttagcaaat tggaatccct acacaattaa aagtcagtta ggtgtaatcg
     2041 gactccagtt atatttccat accaatggaa ttgatcgtac ggatattgct attccaccag
     2101 aaccaaccaa agaaaagtct tatgggaatt cacaagttct accaaaagat tatacgagga
     2161 gagaagaagt agaactagta gtcaaggaga tggcagagca agtagccatt cgaattagac
     2221 aacacaattg caaaactggt tgtgtacgct tagcaattgg tacgtcaatt ttggaagaaa
     2281 aatcaggttt ctcgcaccag atgaaaatac caattacaga taatacgaaa gaattacaaa
     2341 attattgtct ttttttattc aataagtttt atgaggggca ggaagtacga catataggag
     2401 taacttatag cagattagta tacacagatt ccctacaatt agatttattt agtgatccaa
     2461 gaaaaaagat tgctgaagaa aatttggata ggattattga taagattaga caaaaatatg
     2521 gatttacagc tattatacat gcttcatcta tgttagaagg tgcaagaagt attacgagaa
     2581 gcactttaat aggaggacac gcaggaggat taggaggttt gcataatgat tgaatataac
     2641 gatagaggac gtttaaaatg gtcgggattt tatttgtctg atcatactga aagaatcaat
     2701 gcagacaatg tacaacgtgc taatcataat ttggcaaaag aacaaatgac gactgaagag
     2761 attgcgaaaa tactgagaac tgctgtcata aaaaatagac ccgtctgcat tcaaaaagag
     2821 gaacgaaacg ctgaaggtta ctatccagat gatgtagtag gaaaaattat tggatatgac
     2881 gagcttggtg tatttgtgga agaagaaaaa gttcactatg atgaaattag gaatgtttct
     2941 ttctcggaat tattaaaatg gagtcagata tgaacattat taaaaaatac gaattcggtt
     3001 atattacgta tgaacagcta atagaggaat tatggggata tggtcaacaa ttaattaatg
     3061 aagtggggat tgattgcttt tgtttctata ttgaatccgg agcaggatac cataagaaca
     3121 gatactatat atttccatat ccaccaaaat agtctcttag atatttttaa attttctcca
     3181 aatactttca attattgtga aatatttccc tttaattaat caaatttttt ataaaagtaa
     3241 tatataaaat aaattgcttg aagtattata tttagatatc ataatttatg gaagaaaaat
     3301 taattaaaaa ggatgagatt ataatggggt tactggttcc gagagctaat acttatacta
     3361 tacctttaac gaaacgccaa ttaattgcta aaagatatca aaggattact agagccatca
     3421 atagagagtt ttggaattct gaaagtgata ctgctcacag tttatacgta ggttcttatg
     3481 ggagaggaac ggcaattagt actagtgata tagatataat agttgaatta ccgatggcag
     3541 aatttgacag attcaaaaat tatttatcaa atggaccgtc taagttatta caagttataa
     3601 aaaacgcatt tcaggaaata ctaccaaata gtgatattag agctgatgga caagttgtta
     3661 aaataaattt ccatgatgga atcaaatttg aaatagttcc agcttttaat gaaaaagatt
     3721 atttgggtga gtcaaaaggg tttatttatc cagattcaaa catgggaggg aactggaaag
     3781 ctacaaatcc caaaaaagag caagaagcca tgaaattaaa gaatacaaaa agtaataatt
     3841 tattatatgc tacgtgtaaa cattttcgac atgtacgaga tacagaattt acgagttatc
     3901 atctttcagg aatagtaatt gacagtttcg tatatgaggc tatgggaaat tggaaattcg
     3961 ttgaaaataa cagtgggggt caaaacatta gttctgtatc ttatgagaca gctttattag
     4021 aatactataa ctcacataaa gttatgggcg
//
LOCUS       DAPBCS010000106         3853 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPBCS010000106 DAPBCS010000000
VERSION     DAPBCS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.110"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..189
                     /locus_tag="RD521_002815"
     CDS             <1..189
                     /locus_tag="RD521_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772467.1"
                     /translation="LQTIHESINCVVADIEIIDKLLIMDDGKYNIAVTGHFNKRNQLV
                     IESMQIRNPDHFTRSMGI"
     gene            626..832
                     /locus_tag="RD521_002816"
     CDS             626..832
                     /locus_tag="RD521_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDT7772468.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            832..1083
                     /locus_tag="RD521_002817"
     CDS             832..1083
                     /locus_tag="RD521_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772469.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            1098..1535
                     /locus_tag="RD521_002818"
     CDS             1098..1535
                     /locus_tag="RD521_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772470.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            1528..2235
                     /locus_tag="RD521_002819"
     CDS             1528..2235
                     /locus_tag="RD521_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772471.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            2616..2795
                     /locus_tag="RD521_002820"
     CDS             2616..2795
                     /locus_tag="RD521_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772472.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            2926..3195
                     /locus_tag="RD521_002821"
     CDS             2926..3195
                     /locus_tag="RD521_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7772473.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            3188..3445
                     /locus_tag="RD521_002822"
     CDS             3188..3445
                     /locus_tag="RD521_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDT7772474.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT         1421 a          584 c          766 g         1082 t
ORIGIN      
        1 ttacaaacaa ttcatgaatc gattaattgt gtagttgcag atattgaaat tatcgataaa
       61 ttgttaatta tggatgatgg aaaatacaat attgccgtca caggtcattt caataaacgg
      121 aatcaattag tcattgaatc gatgcaaatt agaaaccccg atcattttac gcgatcgatg
      181 ggcatataaa agaagagctg cagtcctaaa tgaggattgc agctcttttt gttttacata
      241 gaaacaggaa aagacctttt taggaagaca caagtgcctt ctcaattgaa aaacctttct
      301 ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt gaacgttgca cgttcactaa
      361 gatatggtgt cttgcaaagc aagacaaaat agaaaaaact tcctctcacc aaaaggttcg
      421 ttcctgttcg ctatctcttt attgatttgc cagaccggca acaagttgcc ttttatcacc
      481 aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc ccttgtcctt gtcgcttttc
      541 gttgattcat tctggccaac aaatcaataa agaaagaact aacgaaagga aataaaacct
      601 ttcctaagag gaggcagaat caatcatgcg aaagaaattt gattattggg gtgttccctt
      661 cagcgaatta ttcccaaact atcatgcacc acatactgtc gaatgcgatt gtggagaaag
      721 agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca acatgcggga aaaaatatac
      781 cttgtcttat ggcgattatg ttttgattga tgagaaagta aagaaacggt gatgaaaaca
      841 caacacaaat tattgactgt aaaagaagtc gttcaaattt tggtggacaa agaagtcatt
      901 tctgaaaaag tagcagaagc tttacttatt ccgcggtact ggacgataga aaccccttta
      961 gtagaggatg caccgactat gctatttgaa gacctagtga agattgcagg atatgaactg
     1021 cattatcaag aaaacatgga aacttatgaa atcgaatatt ggttaaccaa attagagaac
     1081 taaaaaggag aaaaaatatg gctcaaaatt actatgacga atttgtcaaa ttacctttgg
     1141 ataaaatggc acaaaaaatg gaggatatga cgttcctata ccatgaaaca cgtgtaccca
     1201 aaaaacatta caaggaaaaa ttgtcagtcg cagtggaaga aatgattgaa tcaggtgtcg
     1261 aaatgaatct cattgcgacg tactatcgaa cactggaaga actgaaaaaa caaaatggaa
     1321 aatggttttt ccaagcctta ctatgcttgg aagtaggtgt caaaccaagc actatcaaac
     1381 cttccgaata tcaagcctta gaactgactt atgcaaaatt cgcacagatt aagaaagcta
     1441 aagcaatggc tagcgactgg ctagatactt ttgaaaatat cgaaaaaaat ggtgcatttt
     1501 atacgattga atcggaggag aaagataatg aataaggtgg tttatcagct aacaacagaa
     1561 gatgtttcgc aatttttaac aaacaaagaa cacttgaatg aagtaatcag acggattcaa
     1621 aaagacttta gttgtatgga agaaatgaag tactttattc aaagagtgga agaacagttg
     1681 tatatggaaa gtgaaacgag tcaaacgaag gttacagtca gccataatcg tttagacgtg
     1741 tataaacgtc gtcctgacac attaaaaaaa ggagcggagc ttctacgact agaaacagaa
     1801 aaatatatca tgaatgttta cctagaaagg agtgagctgg tcgaaaactt tgtcagctac
     1861 acgatttgca ggaaacacta ttactcagaa tcagaatatt attcaatttt tgagagcatg
     1921 gaattaatgg aagaagaaat ggaagagcta aacagcaaag atgcgacggt agacgattat
     1981 taccttgtat ttactagatt tttgacgtgt gggacggaat ctatcgtgtt aatgggaatg
     2041 gattatgaac tcactgtacc cgaagcattt ttgagggaat atgtagaaga agtagataac
     2101 ccaggcgacg actggtacac gactttaact gattttttag acacctacac ttacgacgat
     2161 acagaagaaa tccaaaatga acttgaccgt aaacgaatgc catatcaacg atttgaaagg
     2221 aaaaaaggtc aataaccatt tataccgaaa agtgaggatg tgtgaaataa gaacaaaaaa
     2281 ccttgaaatt aagggaatgg ttcttatttc gcaccaatcg atccaaccgc ccccgccgct
     2341 taggtggtgc gaaataagcg ggcaaagccc gataacctaa gagaaagaag gagatccata
     2401 tgaataaaat cattcctcta ttgttcgtac tgtttgttgg cgagatactt atgcgtcatg
     2461 gtgcagctct acatttaaac ttcgtagtat tcttaggaat actggtattt tgtttgatct
     2521 tttggcgctt tgtgttaacc cttgtgagtc taatattcaa gttgattgca gccatatttt
     2581 taatagggtt atttcgatga aaggagagag tactaatgcc aattgcaata ggaaataaac
     2641 ggttacccgt gacgttggat gaaaagagac aaaaagaatt gcagcaacta aagcagaagt
     2701 acggcaaaag tgaatccagg attatgtgta ttgcgttaga tttattgatt gcccaagaaa
     2761 aagcaggatt tgaggtacca gcactcaaaa agtgacgtca ctttttatcc taaaaactaa
     2821 aagtgatagc acttttaatt ataagaagtt agaatattaa tcatttgctt aattgtacaa
     2881 tataatgtac aattgtttta tagaaataaa taaggggtga aaggaatgga agcagtagct
     2941 tattcaaatt tccgccaaaa tttacgtagt tatatgaaac aagttaatga ggatgctgaa
     3001 acacttattg taacaagtaa agatgtagaa gatacagttg ttgtattatc aaaaagagat
     3061 tatgattcta tgcaagaaac gttgagaaca ctttctaata attacgtcat ggaaaaaatt
     3121 cgtcgaggag atgaacaatt ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt
     3181 gaatctgatg attaaggctt ggtctgatga tgcttgggat gattatcttt attggcatga
     3241 gcaaggaaac aaaagcaata taaaaaagat taacaagtta ataaaagata tcgatcgttc
     3301 cccctttgct ggattaggaa aacctgagcc attaaagcat gatttatctg gaaaatggtc
     3361 cagaagaatt acagatgaac atagactgat atatagagtt gaaaatgaaa cgatatttat
     3421 ttattctgca aaagatcact attaaccaat cggaagtaag gaaagggtca gaaacttaaa
     3481 agtttttgat ccttatttta tttaccctag tcatttaaaa agctaatata gcttagtgtt
     3541 gattgttatt aatgaatgtg tttgttacgc gtattacgga tataaggtta gtaaaatcat
     3601 ttctaaagtt gaggaaaagt aaatataaat ggcttaaatt tcaacaattt gaagttgaat
     3661 agatatgtta taatactatt gtagtgtggg atgttagtta ctaaaggatg acgcttatat
     3721 atatgactga atagaataag caataggttt aataatctat tttaaatttt ttgtactagt
     3781 tttagttaat tagcaaaaac aacaaaaata aacttctcat agaatttagc taaaaattaa
     3841 tgatttattt aca
//
LOCUS       DAPBCS010000107         3851 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPBCS010000107 DAPBCS010000000
VERSION     DAPBCS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.111"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..651
                     /locus_tag="RD521_002823"
     CDS             <1..651
                     /locus_tag="RD521_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772475.1"
                     /translation="LSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSK
                     LFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTL
                     FGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGI
                     RSTLKDGQETKTIDRRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            695..964
                     /locus_tag="RD521_002824"
     CDS             695..964
                     /locus_tag="RD521_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772476.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1196..1492
                     /locus_tag="RD521_002825"
     CDS             1196..1492
                     /locus_tag="RD521_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772477.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1494..1916
                     /locus_tag="RD521_002826"
     CDS             1494..1916
                     /locus_tag="RD521_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772478.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1935..3527
                     /locus_tag="RD521_002827"
     CDS             1935..3527
                     /locus_tag="RD521_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772479.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3548..>3851
                     /locus_tag="RD521_002828"
     CDS             3548..>3851
                     /locus_tag="RD521_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772480.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1515 a          551 c          755 g         1030 t
ORIGIN      
        1 ttaagtgagc caaaacagat cgcggcgtca aatattttca cggcagtaat gacattaatg
       61 tgtgtcatgt gcttttctat ttttagttac ttactcgtcc aatttatcag gcaaaattta
      121 acaatgagta aacttttcaa aagtttattg gacttactta tcttaggcag tatgttaacg
      181 actctaacag atcggttcga acaatctgaa aaaacggtag aggggataaa gaacttctta
      241 acgacgattg atcaatttat aaatggatat atgtatttgt ttgaagtagg ctttattctc
      301 actttgtttg gttgtgtatt tataaaactc attaaaagat caatcgaaaa agaaatggtt
      361 attgatctaa ttccgacgct gagcggctat gagtattacg ttgattttgg aaagtatgag
      421 gagttgatcc atacggatat cgttcacacg agtatccatg aagtaaccgt taagggcatc
      481 agatctactt taaaagatgg acaggagacg aaaacaatcg accgaagaaa agaggtctac
      541 aatttttata aagttgaagc aagtctacga tatggcattg ggaaattgag gttagatggg
      601 agtaatattc aacaaaacaa accatatgtt ttaggaaaag tggtgattta gaacttccta
      661 tgaaagatga ataaataagg agaatctttt gaaaatgatt cttctttaca gaattgtgct
      721 tatagttggc acggagttaa aaatcttaaa gaaggagcag ttattcaaag tgaaagaaca
      781 tcataatcag ttactatgta ttaaaacggg tacgatgaac attcaatacc gaacagaata
      841 tttagaatta acgaatcaaa ctgatttttt gtcgattcga gagcaacata agcaagaaaa
      901 attaatggct acttatagcc aattgattga tgagtgggaa caattcaagg aatctaaaac
      961 ataaaattcc atttaaacac cttatttttt aaagagcata aggaaacgtg accccacttt
     1021 taaaaaaagt ggttggatag gctgtgccta tcacgtttct gaaaaaaata aatgacccca
     1081 aaataccaat atataggata gtacaaggat agcaaaggat agtaaaagga tagaggtagg
     1141 atagcgaaaa ggataggatc cttttcacta tcctatatta tattggagga tatttatgaa
     1201 tgttaaaatt agagatttag atccaaaatt catttctgaa attgatcgaa gatgcgttga
     1261 actaagtaat cgtacaggaa ataaatggag tcgtaatgat tatttaaagc tactcgttga
     1321 aaatgatttt gatcgtcctt tgatggaata caaacaagaa aagtttgacc agttattaga
     1381 aaagttttca gaaattcaaa gttataacac aaagatatta gaagaatatg ttagccaaaa
     1441 taatcgtatt atagaaatct tgattgagca aaatagagga agtgaacttt gatatggcaa
     1501 aggttataga caaacacatt tactttcacc aaacacatat cgatgtattg gatgaaacta
     1561 tttctactca tgcagaggtt aaaaactata gtgaagctgt tcgcttttta tgtatgaact
     1621 atgatccaga agtggagaaa aaagtaaatt ccaatcaagc aaagttgaat ggaatgagta
     1681 aagaaatttc tattttaacc gaaatggttt cagaatttgc ggatcttcat ttgaaagata
     1741 caggaatatt aattggtaaa aattcaactg tttatcaaga agcaaaagaa cgagtggaag
     1801 gaaaaattaa atccaatcaa acaaaaaaat attcccctca gttgaagaaa cctgtgaatg
     1861 aatctgttca tgccagagta gatccatttg atccaacgaa gattctaaaa agataaaaat
     1921 gaaggaggat aataatgact caatcgcctt cagttattct cacttgtcaa tttactttgc
     1981 cgaataaaaa gtcattttct acttatattg actatattac aagggaacag gctcttgaag
     2041 aaaaggaacg acgtacgccc gaagaagaac aggaacttca acgaatcaag aatgctttag
     2101 aggagtttta tattcctgaa ggagaaactt tttcggagag taagacgggt aaagaattca
     2161 gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt gacaagttag
     2221 aggaacaaga ttttaccaat tatctttctt atatgacacg tcactatgcg ttagaacaaa
     2281 agaaagaact gacgcaaagt gaacaaaaag aataccaacg cttgaaccaa gcaatgaaaa
     2341 aatatcaaac agaaccccat aaaaaagctt caggaaatct tcctggagtt ttttctatag
     2401 cttcagacga agtgaaagga agtgatctga aggagattaa gcaaatcttc caaagaggac
     2461 aacaaaaagg ctcaatcatt tatcaagatg ttgtttctca tgataattcg tatcttgaaa
     2521 aaataggatt gtacgatccc aaaacagata ctttagatga agaagggtta aaagcagctg
     2581 gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca ggttactgga
     2641 tggcaacgat tcaccgtaac actaaacata tccatattca ttttgcagtt gttgaaaaag
     2701 aaaatacgcg taaaccttac acggaggttg aaaatggtgt gagttatatc gtaccgaaag
     2761 gtaagagaag acaagcaaca ttagacgcaa tgaagaccaa ttatgtacat gagttagaac
     2821 gtttttctta tgaaaaggat cgaatgattg gtatggagaa acgaaattta ttgacgcgga
     2881 aaagtgattt gcgtaacacc ttaacaaaag tggtaaaaga gccagcgcgt tacgatcgac
     2941 acgcgatgaa cttattaaat gaagtatata aaaccctacc tgacaaacgt tcggaatgga
     3001 attacgggga tgaaaaacga accaaacttt caccatacac aagagagaaa ctcgatgagt
     3061 taaccaaata tgttttaaca aaagaacctg aatatcaaga atatatacag atttcaaaag
     3121 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     3181 agaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccatccta acagagctga
     3241 agagacagga tagtcaaata aaaccattaa tagaaagtgt tgatcagatg caatctattt
     3301 acaatgaacg atcctttcga acgcttaaac aattgccaac gaatgaatac aaatatagtt
     3361 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg cagcggagtt
     3421 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     3481 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaaaga cagtaggagg
     3541 aatagcaatg aaacaatatt tagatcaatg gaaagttata gaaggatcat taagagaaga
     3601 acgaatcgaa caacttcctg attgtttaga aaaagagcat ttgttccaga taagagaaat
     3661 gttgagaaac gaacagtttg atccaaatca atttttagtc gttgagtacc cagctactgg
     3721 tgtgtattgt tgtaatcatg tgaatggtga aaaatatttt atcattcaag aatatgaggg
     3781 caagttagca ccttattata ctacttggga aatgaatgaa gagggaatca ataactttcc
     3841 atgtaaaagc a
//
LOCUS       DAPBCS010000108         3662 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPBCS010000108 DAPBCS010000000
VERSION     DAPBCS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.112"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..275
                     /locus_tag="RD521_002829"
     CDS             <1..275
                     /locus_tag="RD521_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HDT7772481.1"
                     /translation="LIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINE
                     LIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            418..639
                     /locus_tag="RD521_002830"
     CDS             418..639
                     /locus_tag="RD521_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7772482.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            655..1497
                     /locus_tag="RD521_002831"
     CDS             655..1497
                     /locus_tag="RD521_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772483.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1560..3362
                     /locus_tag="RD521_002832"
     CDS             1560..3362
                     /locus_tag="RD521_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDT7772484.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3421..>3662)
                     /locus_tag="RD521_002833"
     CDS             complement(3421..>3662)
                     /locus_tag="RD521_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7772485.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1306 a          519 c          745 g         1092 t
ORIGIN      
        1 aactcatcaa gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg
       61 agctgagaga caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg
      121 agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg
      181 atgataagct tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca
      241 tatccttcaa atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgat
      301 tctacagtag agtcgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta
      361 ttgttattat aaaccaaatg atttataata ttcttagcgt aaacttggag gtgctgtatg
      421 actacggcag aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt
      481 gctagacgta ttggacagac tccacagaat ttcaataaga aattacaacg agaaacggta
      541 accttagatg agttgaaagc gattgctgat gtgctaggtg tcaagtttgt gcaggctttt
      601 atattgcctg atggcaatga aataaagata ggtaattagg aggtgtaatt tgtattgaaa
      661 atcataacaa taaatgatgt tgagtatgct gtatttgctg ctaatgaagg tacttccaaa
      721 ccacaacctc atatcataga aacgaaatct ggtactattc cagaaggaaa acaactgagt
      781 ttattaaaag aatatcttaa gcagaatgat atttccccaa taaaaggagc aacaacatat
      841 tggtgcattg acaaggttct taagcttgat tcaagtaaag aaaaaactat aagtgaaaca
      901 atccataaac aaaaatactt atctttaact gaagaaaata ttgagaaaca gcataagttt
      961 gtaggtgcaa gctctaatta tgggaaagaa ggtctaataa tacatgatgt tttgaatgca
     1021 tttcctcttc ataatgattt aaatactatt gcgatgaaga ttgcggtaat tgatgtaaca
     1081 aattcaacac atctttctca atataagagc aggttgtctc tatatgattt ggctaaagta
     1141 attctggaga ttccaaattt tgatgatcgg ttggcaaaag gagatcctca gttgataaat
     1201 attatcgcaa ggaacattgg tgcagttaat atgttctcat ttgcatccaa gtactgtaca
     1261 tatcataatg tggaagtgtg tgggagagat gattactcca tctttgatgg tatcgttaaa
     1321 aatactcttc cacactatat ccaaggacta actacgaata agattgatac gtggcgaaga
     1381 agttttgatt acgaggcatt taacgaatgt gttggaaaac tacttgatga aaataatata
     1441 catattcctt ttagaagaag aaagttagat cacttcttgt ggtatgcgaa taggtagaat
     1501 ataagaaaaa ggcatcgtaa aagtgattct ggataaaatt aatgggggag gaaataatag
     1561 tgggattatt tgattttcta aagaaaaaac aaggaaaggt tagtaaacac gttgattatc
     1621 aactggcttc tagtctattt cgtactggta aagtttttgc tgaaaaattg gaagtggaca
     1681 tatcaaatgt agatgagttt cgtaaaactt ttatcgcttt tgatgtggaa acaactggcc
     1741 taaattcttc ttcagataga attgtagaga ttggtgcggt gctatttaaa gaaggagttc
     1801 caattcaatc attcggaact cttgtcaatc caaaagttag aattgcagcc tcagcttcgg
     1861 caataaataa tataactaat gaaatgctaa actctgctcc aaatgagcag gaagtttacc
     1921 cccagttggt agaattttta ggagatgcac tagatggaaa aatcactatg tgtgcacaca
     1981 atgcaagttt cgattttgga tttttaagta acacattttc tagacttggg tatgatgcta
     2041 aaattaagta tgttgatact ctgagtctct ctagaaaaca tataaaaggc attgtaaatc
     2101 ataagcaaga gactgtagcg gaatatttcg gattaataaa tgacactgcc catagagcgg
     2161 aatcagatgc agatatatgc ggaaagattt tttggggtgt tttagatatt attgaggagt
     2221 cgttggaaga acaaaaaaag caaatagaaa agatgactcc agacataaaa gaacttgaag
     2281 cttgtgcttt tattcaagat attattgaga aaaaaggatg cgacaccagt tggatcaggt
     2341 atcgaaaaaa cagtaataat tatgtcgatg taacttgtct atatacattc ttaaagttta
     2401 agtttgctaa gaagggaaat tacatcatta ttagaaaaga tgctacaaat ggaattgatt
     2461 tacctataga accttgtact gtaagtgaag gcggtacaac aaatgtgcga gtctattttc
     2521 gtagcccctt tgacttaacg cctctttcaa attatatttt cagtgcttat agcgattgtt
     2581 ataattctat gaatgaatat atgagtgtta gtaattacac taaaaaagaa gcagagcaaa
     2641 gccttagtat gctgaaatct atttcaaaat cagcgatgaa agagttatta ttcgatgcaa
     2701 aaaatcgtgg atatgatgag actgaagtta acctaaatat tgaaaaagtg atatctagag
     2761 aagatgtaat tattaacggg ttgcataatc gagtttcatt aaacaagata cgaaatatag
     2821 ggaattggga gaaaggattt gatgcaggtt atccatattg ggaacgtgga gaaactgcta
     2881 ggaaaaatgg caaagtagat gaagctattg cactttttga taaagctcga tataacggct
     2941 atgatgctcc tgcgctatat gattcttatg ctaaagctta tcgaaaaatt aaggattacg
     3001 ataatgaaat tctcattctt gatgaaggaa ttatgcgtaa aacacggcac gatgttggta
     3061 ctttagaggc tcgtagggat aaggcaatta agctattatt tgctaaacaa gaggcagagc
     3121 ggatagctaa ggaaaaatct gattttctaa aagcgaacaa aaaagaggat gcgattagtg
     3181 agcctagcaa gaagcgtgga agacagatta ttcaaatgac tgatgatgga acaataataa
     3241 aagaattcga tactatagct tctgctgtaa aagaagttgg cgttaactca aaaagtatta
     3301 gggatgctgc aaatggagtt caaaaacatg ctggaggata ctactggaaa tacaaagatt
     3361 agcagataat ctaagataac ctaggatctt cttaatagat acccgtggtg ctagttaatt
     3421 ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag
     3481 aacactgtaa gccagtaata tgctttcaaa tctactctct agccctttaa cagaacgtga
     3541 gttgaaattt tgacaaccta atttttctaa ggaagaaaaa acagtcccaa ctgtttttct
     3601 ttgttttcca agtaagctgt ggtctagttt ttctgattta tccatgtttt tacgtggcgg
     3661 aa
//
LOCUS       DAPBCS010000109         3657 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPBCS010000109 DAPBCS010000000
VERSION     DAPBCS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.113"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..748
                     /locus_tag="RD521_002834"
     CDS             137..748
                     /locus_tag="RD521_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDT7772486.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="RD521_002835"
     CDS             821..1750
                     /locus_tag="RD521_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDT7772487.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="RD521_002836"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="RD521_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..>3657)
                     /locus_tag="RD521_002837"
     CDS             complement(2693..>3657)
                     /locus_tag="RD521_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDT7772488.1"
                     /translation="LHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSE
                     IADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTV
                     AKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKG
                     MAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARG
                     AFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKE
                     QFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1192 a          578 c          713 g         1174 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagtt
//
LOCUS       DAPBCS010000110         3633 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPBCS010000110 DAPBCS010000000
VERSION     DAPBCS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.114"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(132..716)
                     /locus_tag="RD521_002838"
     CDS             complement(132..716)
                     /locus_tag="RD521_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772489.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(927..1130)
                     /locus_tag="RD521_002839"
     CDS             complement(927..1130)
                     /locus_tag="RD521_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772490.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1140..1562)
                     /locus_tag="RD521_002840"
     CDS             complement(1140..1562)
                     /locus_tag="RD521_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772491.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1800..2414
                     /locus_tag="RD521_002841"
     CDS             1800..2414
                     /locus_tag="RD521_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772492.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2579..3403)
                     /locus_tag="RD521_002842"
     CDS             complement(2579..3403)
                     /locus_tag="RD521_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772493.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3427..3582)
                     /locus_tag="RD521_002843"
     CDS             complement(3427..3582)
                     /locus_tag="RD521_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772494.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT         1203 a          635 c          517 g         1278 t
ORIGIN      
        1 aatattaaca aatgaataaa atccttacaa catttgagaa taagttatca gtagacactt
       61 acaaaaaatt ctcatactat tgtcactttt ctagataaat ggcaaaaatt ttcagcatta
      121 aatgacaaaa actaatgaga atttgtcttt ccatttttct cctccatttt tgccaaataa
      181 cgataaatgg tggttcttga gacgttccac cgttccgcaa tagtcttaat aggtgtaccg
      241 ctatccacta aagtcttcag taattccaaa tcttgttcag acaatttttc aggacgacca
      301 ccaaaccgtc ctcttgcacg agcagctgct cgtcctgctg cggatcgttc caggatcaaa
      361 ttacgctcaa attctgcaaa tgccgcaaac aaatgaaaca tgagctgtcc tgtagaactt
      421 gatttgtcca tcgtaatatt ttcttgaata ctatgaaaac tcacaccacg ctcattcagt
      481 cgattgacga ttgaaatcaa atcttccata tttctaccta aacgatctaa gcgccaaacg
      541 acaagtgtat cgccagagcg aacaaaatca atggccattt ctaatcctgg tcgattactt
      601 tttgccccac tcatatgatc agtaaaaatt ttttcacaac caaatttatt taggctgtct
      661 tcttgtaaat ccaaattttg taaaccagta gatacccggg cgtatcctat tttcaaaaaa
      721 atcactcctt cattaatttt attgtaacat aactttatcc atatagttta actgaacata
      781 gatattgtta tgggtttatg aacatgaaat gaattgtttt tttgaaaaat aaagcaaaaa
      841 aagaaagtgt ccagaaatgg gtgattttat gaacactttc aattagaaaa aaagaatgtc
      901 gactagacac tcttttttta aaatcattat ttttcttttt gatatgtttt ttggtctctt
      961 ttttttcttt tttcttcaga atcttctaga ttagaaattg atttttgaat tttttgtgtc
     1021 tcctgtaaag ttcgctgaac ttgtaaagct gattcttctt tcggtaaatt aattttactt
     1081 agaatatcgc tatcttgata aaaatttggt gtgttcacaa attctggcat aaaaaatccc
     1141 tactttctag ttaaggttcc tgaataagtc gcaatccaat tgtttccagc tgatttataa
     1201 ctttgtaaat acaattttcc atagtaccaa taaccatttc ttttttcttt ccaattccgt
     1261 gattgaggag gtctagaagg ccaaccaact ttatcaaaaa tcatcgttcc tgtcactcgt
     1321 atcgttttag aagctgctag agcaagtaat ggagaaacta ttaaataatt ttcagtaaca
     1381 gttaagtcgt tagttgggtt accaatggct attccgtccc tatctaaagg agttaattct
     1441 tggttattat ctactacagt agttggttca tttgtactat cagcaaatgt aggaattgta
     1501 gttccagtta cactaattcc aatagttaaa atacctaaag ataaaataaa cttatttttc
     1561 attgtctatc acctcgaaat caaaactaag aaaacgtttt cttaataaat cttaacagat
     1621 aaatcaagtt atatactaga aaattcataa aatatatttt taatgtcata atcgtttaaa
     1681 ttttcttgat tgacatttat ttacaaaaaa tattgccatt taaaaagaaa agtgacatat
     1741 ggcttcctcg tgtatgatta gggtgtattt taaatagttg cagaaagtag gtaaaagttt
     1801 tgagagttgc atacatacgt gtctcatcga tcgatcaaaa tgaacaacgc cagattgaag
     1861 aaatgaaaaa attcggtgca gagagaattt tcatcgagaa acaatctggc gcaacgatta
     1921 ctcatcgacc tgttttccaa gaagcattag attacgtccg agatcaagat atttttattg
     1981 tggaagctat tgatcgactt ggaagaaatt atgacgagat catcgcttca gtcaattact
     2041 tgaagaagaa aaatgtgaag ctgatcatta ctagtttacc gatcatggct gaagcaattg
     2101 gcaatcctct cctggataaa ttcattaagg atctgatcat ccaaattttg gccatgatcg
     2161 ctgaacaaga acggactgaa tcgaaacgtc gccaggcaca aggaattaag atcgcaaaag
     2221 caaacggcgt ctacaaaggg cggcccaaat tatacagtgc agacgcaaaa gatccccaac
     2281 gtcgccttgt ttacaaaagc atcgttgaag atcttaaaaa tggtgttgcg attgctaaga
     2341 ttgctaaaga ctacaatgtt acgagacaaa ccgtgtatag aataaaaaaa gacagcatgg
     2401 tcaatgacaa atagttgatc atgctgcttt tattatcttc tcatctttaa tttcctacat
     2461 ataattacta tctataaaat taagtcttac tcagaacaga taaagcataa aattccaaca
     2521 aaccattttt tacttaacta aataaagagt ctgttaatat aatcttcact tatttacgtt
     2581 aatcccctac tgtatttgtt ggttccttat ctatattttc tacaaaatcc ttattatctt
     2641 gtgatttagc ttcgtataat tctttgtatg tcgacacatt ggttatacta attcctatag
     2701 ctagttcttg agactctact tttctctcta gctcctttga caaagtgatt tgagatttat
     2761 ttggttccgt tggagtcaat tctatagatt cttcataaaa atctctatac ataaagctat
     2821 cattaatttt ataaaaaaaa ttaaatgttt ttagcactgc catataacat tcaaaacatt
     2881 cttctatagt tggtttactt ccttgaggag aatgaaccaa tttattacgt ttttctatga
     2941 aatttttaat ttcctttctt atttctttcg gaaactcaat attaaagtac cctttaaaca
     3001 tgctccctaa taatttgtct acagggggag attgtaaatt ctcaaatatt agagttaaat
     3061 caggtttctc ttgttgataa aattccttta cccctatttc acaggctgta attatcgaaa
     3121 taaacctatc tctagtattt gatatatttt tacttctttt aaggagtata agagaaaagg
     3181 ggatactttc gtgtccatgc atgtaattaa ctaattgttg agcttcagaa gttatatctc
     3241 taattatata gttaacggaa ttaggaagtg tctttaactc taatgaaatt tctatccaat
     3301 tactatcttc taatctgtat atgctcgaag tataatttga ttcgtcagca agcatcgcta
     3361 catcccaaac actgatcata aaatctttta aacgttcaat catttcagca atattgctaa
     3421 aatcagctaa tttttcttta gaataaaaaa aacatttata tcagaatcaa taaattctat
     3481 tttttttatt actatatcgt catcattatc ttgaccattt atgtttattg ttccctgagt
     3541 ggggagtact tctttcaaat tcaagttaaa tttatattcc attacttctc ctccaatata
     3601 taaaaattac ttaactgtat ccaaatttaa tat
//
LOCUS       DAPBCS010000111         3518 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPBCS010000111 DAPBCS010000000
VERSION     DAPBCS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.117"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..2077
                     /locus_tag="RD521_002844"
     CDS             <1..2077
                     /locus_tag="RD521_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDT7772495.1"
                     /translation="VFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNV
                     TNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDA
                     DKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIE
                     VTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGT
                     TVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKV
                     TTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEV
                     DVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSG
                     KNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLG
                     YKNRKKENE"
     gene            2211..>3518
                     /locus_tag="RD521_002845"
     CDS             2211..>3518
                     /locus_tag="RD521_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDT7772496.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT         1381 a          500 c          712 g          925 t
ORIGIN      
        1 agtctttgca gtgaaagacg gcgtaagctt accagaagat aaacttccag tgttgaaagc
       61 aaaagatggt tatacagatg cgaaatggcc agaggaagca actcaaccaa ttacagcaga
      121 tgatacagaa tttgtatcaa gtgcaacaaa actggatgat aaatctgacg ctgacaaata
      181 taatcctgaa ggtcaaaaag tgactacaga attgaataag gaaccagacg catttgaggg
      241 aattaagaac aaagaagatt taccaaaaga tactaagtat acttggaaag aaaaagtaga
      301 tgttagcgca gctggaaata aaaaagggac tgttgtagtg acatatccag atggatcatc
      361 tgatgaagtt gaagtagatg ttacagtgac agacaatcgt tctgacgccg ataaatatga
      421 gccaacagta gaaggcgaaa aagtagaagt tggaggcaca gtagatctaa cagataatgt
      481 aaccaaccta ccaacattac cagaaggaac aacagtaaca gatgtcactc ctggtggtac
      541 gattgatacc aacacaccag gtaattacga aggggttatc gaagtaacgt atccagatgg
      601 tacgaaagat acagtgaaag ttccagtaga agtgacagac aatcgttccg atgccgataa
      661 atatgaacca acagtagaag gcgaaaaagt agaagttggg ggcacagtag atctaacaga
      721 taatgttact aacttgccag cgttaccaga aggaacaaca gtaacagatg ttactcccgg
      781 cggtacaatt gataccaaca caccaggtaa ttacgaaggt gtcattgaag taacgtatcc
      841 agacggtacg aaagatacgg tgaaagttcc agtagaagtg acagacaatc gttctgacgc
      901 cgataaatat gaaccaacag tagaaggcga aaaagtagaa attggaggca cagtagattt
      961 aacagataat gttactaact tgccagcgtt accagaagga acaacagtaa cagatgttac
     1021 tcccggcggt acaattgata ccaacacacc aggcaattat gaaggtgtca ttgaagtaac
     1081 gtatccagac ggtacgaaag atacggtgaa agttccagta gaagtgacag acaatcgttc
     1141 taatgcagat aaatacaatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc
     1201 agatgcatct gagggaatta agaacaaaga agatttacca aaaggaacta cttatgcttg
     1261 gaaagagaaa gtagatgtta gcacagctgg aaataaaaaa ggtactattg tagtgacata
     1321 tccagatgga tcatctgatg aagttgaagt agacgttaca gtgacagata ctcgttccga
     1381 tgcagataaa tataatcctg aaggacaaaa agtgactaca gacttaaata aagagccaga
     1441 tgcatctgag ggaattaaga acaaagaaga tctaccaaaa ggaactactt atacttggaa
     1501 agaaaaagta gatgttagta cagctggaaa caaaaaaggt actgtagtag taacatatcc
     1561 agatggatct aaagaagaag tagaagttac tatttctgta gtagataaaa aagcaccgaa
     1621 taaacctcaa gttgatccta ttacagaggg tgaccaaatt gttactggta aaactgaacc
     1681 aaatgcagag gtgacagtta cattaccaga cggaagtcaa caccatggga cagctgataa
     1741 aaatggtaat tttacagtta aagttcctaa attagaggca gggacaaaag tgatagtaac
     1801 tgcaactgat gaatctggta atactagtga acctactaat gtagttgttt cttccaacaa
     1861 aaaagacagt ggaaaaaacg gtagtaaagg tagtaagact gacaatcaag gtagcaacag
     1921 caatcaagac aaaaatagag gcaaaagcca gtcctcaaaa gtttttccta agacgggaga
     1981 gagtgacagt aatatcttta ctattagcgg tggtttaata ctgttaggaa cgttaggatt
     2041 attagggtat aaaaatcgaa aaaaagagaa tgaatagaaa atcaatttct taagtcttct
     2101 ctgagaagag gaagaatctt tttcttcctc tttttgcttt tttatgatgg gaaataagtg
     2161 ttagttaaag atgaatatac tttttataga aaggaaaaga atgtaattaa gtggaaaaaa
     2221 tagtaattat tggttcaaac cattcagggc tagccgcagc tcgatttcta aacttaagcg
     2281 attcaaatta tgaagttatt ctaatcgata aaaaatcaga aattagttac ttggaatgtg
     2341 ggacatcgtt atttataaaa ggagaagtaa gtaatttaga agatcttttt tatcaaaata
     2401 gaatgacact aaattctgag ataaaaaagt tatttctaga gacagaagtt gacaaaattg
     2461 atattcaaaa taaaaagatt agttgtgtaa aaaaagatgg tacaagattc tctcaagaat
     2521 atgacaaagt tatattagct acaggttcaa agcaaattat gctagatata ccggggaata
     2581 atttagctaa tattttttcg ataaagaatt ttccaaatgc aaaagagatt tttaaagctt
     2641 tggatgatag tactattaaa aaagtaggaa ttattggagc aggatatatt ggagttgaaa
     2701 ttgctgaagc cataagaaaa agagggaaag aagtatatct tttcgatgtt gcagatagag
     2761 tgttgtcgac ttattacgac cgtagttttt cagataaagt tgaagaaata ctatcaaaaa
     2821 atggaataca tctttgtcta tcagaaagtc ctaaaaaata tttaggaaga aaaaagattg
     2881 aaaaaatagt cactacaagt ggtacccaat actctatgga tatggtcgta caagctatag
     2941 gatttctatc taatagtcca attggggaaa aggaactctt gagagatgtt agtggagcat
     3001 atctaactaa tgaataccag caaacaaata taaaagatat atatgctatt ggggattgtg
     3061 ctacaacttt ttttacgtcc attaatagaa gtagtgttga tttttctatt tctaatgctc
     3121 taagaacagc ttatattgca actcaacata ttaacaatca agattttaca ccttctggtt
     3181 cccaatttac aaatggcttt tctatattta attacaattt ttacgcagca ggtttgaatt
     3241 taaaaacagc acgattatta agtgagtcta tagattatat agaaatagaa gatttcatta
     3301 gacctgcttt tttgagaaaa aatagtcgtg taaaattaag aattagcttt gaaaaaaata
     3361 caaagagaat agtaggtgta caactttgta gtcaagaaga tttatctgga ctattatcaa
     3421 tgttttctct tgctatagaa gaacaaatta cattagacaa attgaaaatg ttagattatt
     3481 tatttcatcc tagttttagc caaccctata attttatt
//
LOCUS       DAPBCS010000112         3482 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPBCS010000112 DAPBCS010000000
VERSION     DAPBCS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.118"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1125
                     /locus_tag="RD521_002846"
     CDS             <1..1125
                     /locus_tag="RD521_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772497.1"
                     /translation="LYIKDFPEIPYKVFKDIQEKYSDTFSLDKDYELLNPIIKFTIGG
                     VTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRGERNPRNTETWNILADYFHVPV
                     SYLMGLDETNVELKNLNIGNNIKNIRKSKNMTQTDFAELMGLSRSYIGDLENNRSNPS
                     IKTLEAIARALDVPIVALINDSSTDNIVEPYESLTAENILLKQENEELKYEVESLRKR
                     LYEIQQVVNQGAEKMIPNLKDVSKRIKYIRKKLGMTMEQFGSHLSNSPKSTIATWESG
                     RNIPSQKKLQLIAIVGETTTDWIKWGTLEEYITSYLIGIGYELYIKDFPEIPHKVFKD
                     IQEKYSDTFSLDKDYELLDSIIKNIFTKYYSKEFEVTQIP"
     gene            1139..1921
                     /locus_tag="RD521_002847"
     CDS             1139..1921
                     /locus_tag="RD521_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772498.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            1961..2572
                     /locus_tag="RD521_002848"
     CDS             1961..2572
                     /locus_tag="RD521_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772499.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
     gene            3016..3324
                     /locus_tag="RD521_002849"
     CDS             3016..3324
                     /locus_tag="RD521_002849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772500.1"
                     /translation="MKLEIKEVVCDWGIYVDGETYPFMIFNSKANAQEIMRIMELDNK
                     HERFDQVKKMENECEAVEYQKTCANNKYILGAMGLGKSASIKQVYEESQENCVYENSN
                     "
BASE COUNT         1399 a          446 c          638 g          999 t
ORIGIN      
        1 ctatatatta aagactttcc agagatacct tacaaagtct ttaaagacat tcaagaaaaa
       61 tatagtgata cgttttcact agataaagac tatgagttat tgaatcctat aataaaattt
      121 actatagggg gcgttacttt gaatagaata aaattttata ttgattccag taaaaagacc
      181 attaaacaaa ttagtacaga aactggtatt aactatacaa ctcttacgaa ctattaccgt
      241 ggtgagcgaa atcctagaaa tactgaaaca tggaatatcc ttgctgatta tttccatgtt
      301 cctgtaagtt atttgatggg attagacgaa acaaatgttg aactcaaaaa ccttaatatt
      361 ggaaacaaca tcaaaaatat tagaaaaagt aaaaatatga cacaaactga ttttgctgag
      421 ttaatgggtc tatcacgatc atatataggg gatttagaaa acaacaggag taatcccagt
      481 ataaaaacac tggaagctat tgcaagagca ttggacgttc cgatagtagc attaatcaac
      541 gattcttcta ctgataatat agtagagccg tatgaaagtt tgacagccga aaatatcctt
      601 cttaaacaag aaaatgagga attaaaatat gaggtagaaa gcttacgaaa aagattgtat
      661 gaaatccaac aagtagtaaa tcaaggagct gaaaaaatga ttcccaatct aaaagacgta
      721 agtaaaagaa tcaaatatat cagaaaaaaa ttaggtatga ctatggagca atttggttct
      781 catttaagca actcacctaa aagtacaatt gctacttggg aaagtggtag aaatatacct
      841 tctcagaaaa agttacagct gattgctata gtaggagaaa ccaccactga ctggattaaa
      901 tggggaactt tagaagagta tataacttca taccttatcg gtataggata tgagctatat
      961 attaaagact ttccagagat acctcacaaa gtctttaaag acattcaaga aaaatatagt
     1021 gatacgtttt cgttagataa agactatgaa ttattggatt ctataataaa aaatatattt
     1081 acaaaatatt actcaaaaga atttgaagta acccaaattc cataagagag gtgattgtat
     1141 gaagccaaat ccttcagaag caggaaaacg aatcaaacaa ttacgccttt catgtggttt
     1201 tactatggaa gaacttggaa ggaaaataga taattcacca cgtgcgacca tatccaactg
     1261 ggaacgtggg accaatttac caaatccaca aaagctgaaa ttattatcaa ctatcactaa
     1321 ttcaacaatt gactggatta aatggggaac tttagaagaa tatataactt cataccttat
     1381 cgatataggg tatgagctat atattaagga ctttccagaa atacctcaca aagtctttaa
     1441 agacattcaa gaaagatata gtaatacgtt ttcgctaaat aaagactatg agttattgaa
     1501 tcctataata aaaaatatat ttacaaaata ttactcaaaa gattttgaag attatcgaga
     1561 catagaagtt aaaccagacc ctaaaaaggt agggagaaaa attcgttcta ttagaaaaaa
     1621 attaggactt actatgcagg aatttgggta cgaggtttct aattctccta gaagcactgt
     1681 gtcaacatgg gaacatggtg gtaacttacc gaacaaagcc aaactaaaaa aaattgcaga
     1741 cattgcgaat tgtagtgtag aagatttgct ttttgatgaa ggctttattt ctgaaaaatc
     1801 ccaagttaaa ttgataaatg aactcaaaga agaaaattca aagctaaaag aaaaaataaa
     1861 atactatgaa tgtttatttg atggtataga gagtctgctg aactcacgaa aaaattccta
     1921 gttaattaaa ctagagtaaa attatttgta ggtgttaaat atgttagttg gctatgcacg
     1981 agtcagttca gatgatcaaa atttggaaag acagcttgaa gaatttagaa aaattggtgt
     2041 cgaaaaagta tttgctgaga aaaaatctgg acgtaacaca gtagaacggc cagagttcaa
     2101 taaaatgttg tcatttgtca gagaagggga tacgcttgtt tttgagtcgc ttgaacgctt
     2161 aggaagaaat tctgatgaaa tactagaaac agtatctttt atagataaaa atggtatggg
     2221 cttaatggtt ttgaatttac caattttaga tacaaaattt ggtgatccaa acttagaaaa
     2281 acttgttaga agcttagtaa ttaacatttt ttcttggacg gctcaaaatg agagggagga
     2341 aattaagaga aaacaaaaac aaggaatagc aattgcgaag aagaaaggag tctataaagg
     2401 aaaaccctac gaatattctt ctactgcaaa gaatccacaa aagaggttta tctataatga
     2461 aatagtaaga atgcttggag aaggtgttcc gattaagcga attgcagaaa aaacaggaac
     2521 aaatagaaat ttagtctata gaataaagaa aagaataagt gaatctgatt gattttaagt
     2581 tctttgagaa atcaatgtta cgagagaagg attctaagcc tgtttcaaac ttttaaggtg
     2641 tacaattata caaagaagct tctaaacaaa agaattaatt ttgtggcagt caagtaacga
     2701 cagaaataga aaagaaagag gctatgcgag agtgaatcaa caagttaatt gggttaatga
     2761 ttttgaaaaa gaatttcgac tgaaattttt tggaaatcct acagcagaaa gtacagaaga
     2821 aaaaaattat gactggtcag aattttaggt tgcatattcg attcaataag tgttgaagat
     2881 aatagttctc ttacgccaca gtatacaacg ttagaatatg acgaaaacag attcaataca
     2941 tttccttgca ataccattca agaatcaatt gataagcatg agtgataatt gattatctac
     3001 aggaaggtgt tttttatgaa actagagatc aaagaagttg tctgcgattg gggaatttat
     3061 gtagacggag aaacatatcc atttatgatt tttaatagta aagcaaatgc acaagagata
     3121 atgcgtatca tggagttgga taataagcac gaaagatttg atcaagtaaa aaaaatggag
     3181 aatgaatgtg aagcagtgga ataccaaaaa acatgcgcca ataataagta tattttaggt
     3241 gcaatgggat taggtaaatc agcttctatt aaacaggtct acgaagaatc acaggagaat
     3301 tgcgtatatg aaaattcgaa ttaaagagtg agaatatgtg ggagctaatt tatttagttt
     3361 tatacttaat aggaaaatca actgaatttg tatataacaa taaggtaata tttatattgt
     3421 tcttaatagt ttgtattatc gtatttgatg tctatgattt actaattcat aagaatgaaa
     3481 at
//
LOCUS       DAPBCS010000113         3409 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPBCS010000113 DAPBCS010000000
VERSION     DAPBCS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.119"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            81..1253
                     /locus_tag="RD521_002850"
     CDS             81..1253
                     /locus_tag="RD521_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDT7772501.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            1443..2774
                     /locus_tag="RD521_002851"
     CDS             1443..2774
                     /locus_tag="RD521_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772502.1"
                     /translation="MELLEKAYSSTELIDSYEYEDLKYIKDQRNYSAHPVVTYNGNTW
                     ELRDITKETCQDVIRKSYEIIFLKNPILGKKVTIEIIQYASRIHAMSVTPEEFHSALK
                     NSYLNKLSEKAKENLCKTAFKFVFQLSYGNEEADRDRESTFRLLNALIFDNKEFYLNI
                     LKKEIAKYRFTAESREEINKSLGENQQITYTKGFFLLTFIATHPELQHDFSDETIQNL
                     QISINDFKPKQQVLTEIEYYYRLRSLAVLGNNNEELQNHLNSLVDPILQQKMRVSSLP
                     SSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPWIYNKLNQEQFNK
                     LLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYPNAVTMDQGFQLK
                     DKELKKLYELAESKSDMYLDIIDNIINKSEDYLRKEINSEKELLNYVHKIK"
     gene            complement(3031..3366)
                     /locus_tag="RD521_002852"
     CDS             complement(3031..3366)
                     /locus_tag="RD521_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772503.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
BASE COUNT         1292 a          558 c          518 g         1041 t
ORIGIN      
        1 taaaagtaaa aaggccatat aacagtcctt ttacggtaca atgtttttaa cgacaaaaac
       61 atacccagga ggacttttac atgacccaag tacattttac actgaaaagc gaagagattc
      121 aaagcattat tgaatattct gtaaaggatg acgtttctaa aaatatttta acaacggtat
      181 ttaatcaact aatggaaaat caacgaacag aatatattca agcaaaagaa tatgaacgaa
      241 cagaaaaccg acaaagtcaa cgaaatggct attatgagcg cagctttacg acacgtgtag
      301 gcacgctaga attaaaagta cccagaacac gtgatggcca tttttcaccc acagtgtttg
      361 aacgttatca acgaaacgaa aaagccctca tggcttcaat gttggaaatg tatgtatcag
      421 gcgtttcaac tcgtaaagta tcaaaaattg tggaagaact ttgtggtaaa tccgtctcta
      481 agtccttcgt ttctagctta acagaacagc tagaacctat ggttaacgag tggcagaatc
      541 gtttattatc agaaaaaaat tatccttact taatgaccga tgtactctat ataaaagtac
      601 gagaagaaaa tcgagtactc tcaaaaagct gtcatatagc gattggaata accaaagatg
      661 gcgaccgtga aattatcggc ttcatgattc aaagtggcga aagcgaagag acctggacaa
      721 cattttttga atacctaaaa gaacgcggtt tacaaggtac ggaactcgtt atttctgatg
      781 cgcacaaagg attagtctct gccattagaa aatccttcac caacgtaagt tggcaaagat
      841 gccaagttca cttcctaaga aatatcttta ccaccattcc taaaaaaaat tcaaaatctt
      901 tcagagaagc tgttaaagga atttttaagt tcacagatat taacttagcg cgtgaggcta
      961 aaaatcgatt gattcatgat tatatcgatc aaccaaaata ttcaaaagct tgcgcatcat
     1021 tggatgatgg attcgaagac gcctttcaat ataccgtaca aggaaattcc cacaatcgac
     1081 taaagagtac caatctaatt gaacgactga atcaagaagt acgcagaaga gaaaagatta
     1141 ttcgcatctt ccccaatcaa acatcagcca atcgcttaat tggagccgtt cttatggacc
     1201 tacatgatga atggatttat tcttcaagaa aatacatcaa ttttgataag tagaaatggt
     1261 aaaaacattg tatagcattt tacacaggag tctggacttg actcaattac agtgcgctta
     1321 ttttagattt aattgataaa ttatctgatt tagcaaatat ttacgaggat aatacttcta
     1381 agaaaatttt agaagatata gctagactaa gaaaaagtga tccaaactct ccaaaatggg
     1441 aaatggaact actagaaaaa gcttatagtt caactgagct aattgatagc tatgaatatg
     1501 aggatttaaa atatattaag gatcaaagaa attattcagc tcatcctgta gtcacttaca
     1561 acggtaatac ttgggaatta agagatataa caaaagaaac ttgtcaggat gttatacgga
     1621 agtcctatga aattatattc ctaaaaaatc caatattagg aaaaaaagta acgatcgaaa
     1681 ttatacaata tgcaagtcgc atacatgcta tgtctgttac acctgaagaa tttcactctg
     1741 cactaaaaaa tagctatcta aataaactaa gcgaaaaagc caaagaaaac ctatgtaaga
     1801 cagcctttaa gtttgttttt caattatctt atggaaatga ggaagcagat agagataggg
     1861 aaagtacttt cagactactt aatgccctaa tttttgataa taaagaattc tatttaaata
     1921 ttctaaaaaa agaaattgca aagtataggt tcacagctga atcaagagag gaaattaata
     1981 aatcacttgg tgaaaatcaa cagatcacgt atacaaaagg gttcttttta cttactttta
     2041 tagctaccca tccagaatta caacatgatt tttcagacga gacaattcaa aatttacaaa
     2101 tatcaataaa tgattttaaa ccgaaacaac aagtactaac agaaatagaa tattactacc
     2161 gtttacgttc ccttgcagtt ctaggaaaca acaatgaaga attacaaaat cacttaaata
     2221 gtctagttga tcctatactc cagcaaaaaa tgagagtttc ttcgctacca tcttcaacat
     2281 tagaaacaat atataatcaa tatttatatt ttggtgaaaa agagatgttc atagatttta
     2341 tgatcgaaca tcttaccgat gctagttcat tcaggttagc agataaggat atggaagtat
     2401 taccatggat ttacaataaa ttaaatcagg agcaatttaa caaattacta tacaacctaa
     2461 atggaaataa tcaattttac aaaaacagta attttccgcc ttttatttct aatctgttag
     2521 acttttataa tcagaaattc caagtgaatt tacgtaaaca ttatagtggg cttctatatc
     2581 caaatgcagt gactatggat cagggttttc aattaaagga taaagaatta aaaaaactgt
     2641 atgaattagc tgaatcgaaa agtgatatgt atctagacat aatagataat attattaata
     2701 agtctgaaga ctatttaaga aaagaaatca attccgagaa agaattgtta aattatgttc
     2761 acaaaataaa ataatttttt ataacacaag atgcactttt atagcgtata tttactttca
     2821 aattaaacta attagttata ttctcacaat agcatttaat aataaatata ctgtcgcttg
     2881 atttatatat ttccctcttc tttctttaga atctctcttt attttgatat aataaaatag
     2941 aaatcgcacc aaacaatgaa atggcggaaa acatttcatt gaaaaaagga ttgaacataa
     3001 tgttcaatcc ttttttatag ttataatcaa ttaacattcg gtgtgtgaaa ttgaatcttc
     3061 gatgctttca catggaaaat tattgattcc ttcttcactc atttcccatg tagtatagaa
     3121 aggagttaac tgtcctccat actcctgaat aataaaatat tttccatcat tgatatggtt
     3181 acaacagtac actccaatag ttggatactc gactactaaa aattggtctg gatcaaactg
     3241 ttcttttttc aacatttctc gtatttgaaa caaatgatcc ttctctaagc aatcaggtaa
     3301 ttcctttatt ctttgcgtta ctaatgaccc ctctatcgct ttccattcat ttaaatattg
     3361 tttcataaca attcattcct tttatgttaa ctatttttcc taggaaatt
//
LOCUS       DAPBCS010000114         3366 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPBCS010000114 DAPBCS010000000
VERSION     DAPBCS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.120"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            3..587
                     /locus_tag="RD521_002853"
     CDS             3..587
                     /locus_tag="RD521_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7772504.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(663..1856)
                     /locus_tag="RD521_002854"
     CDS             complement(663..1856)
                     /locus_tag="RD521_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDT7772505.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            1920..2300
                     /locus_tag="RD521_002855"
     CDS             1920..2300
                     /locus_tag="RD521_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDT7772506.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2374..2736
                     /locus_tag="RD521_002856"
     CDS             2374..2736
                     /locus_tag="RD521_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772507.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1150 a          554 c          637 g         1025 t
ORIGIN      
        1 atatggcatt cccacaacta gatttaaaaa gtgaaaaagg accaaaagct gttatcaaaa
       61 caaaccgtgg tgacattact gttcaattat tccctgaact agcaccgaag acggttcaaa
      121 atttcattga actttccaaa aaaggttatt atgatggcgt gatttttcac cgcgttattc
      181 ctgatttcat gatccaaggc ggcgatccaa ctggaacagg aatgggtggc gaaagtattt
      241 atggcgaaag ttttgaagat gaattcagtc gtgaactgtt caaccttcgc ggagctttat
      301 ctatggcaaa ttcaggtcca aacacaaacg gaagccaatt ctttattgta aataacacaa
      361 atgtgccagc taatatgctt ggacaactag aaggtgctgg attcccatca gaaatcattg
      421 aagcgtataa aggtggcgga actccttggc tagatttccg acatacagtc tttggacatg
      481 tactagaagg gatggacaca gtagatgaaa tcgcaaatgt acaacgtggt ccacaagacc
      541 gacctgtaca tgatgtagtg attgaaacaa ttgaaatttc agaatagttt ggtatggtac
      601 ggagtaatta tgaaaaagga cgaaacctag taaggtctcg tccttttatt ttgaataaat
      661 atttaatcga ttgtctctaa agcatacttg attcgattaa gtccttccag taaagtttct
      721 tctggacaag caatattcaa gcgcatatgc ccatgaccgg aaggaccaaa cgtgatgcca
      781 ggatttaata cgacttttcc tttccgaaca agagtgttct ccaattcttg gtcgtctgaa
      841 atataggcgg taaaatctaa ccacattaag taagttcctt cagggttcat tattctcact
      901 ttcggaagat actgttcaaa aaactgatta actgtttgaa catttttttc tagataagga
      961 atcagttggc ttaaccattc tccacccgtc tcataagccg cttgggttcc aatcagtcca
     1021 aaagtgttga tttcgtgctg ttggttcatc actaaatgtt tttcaaaatc agctttcatc
     1081 tctggatttt tgataaatac cattgaattt ttgattgcag ctagattgaa agtcttggtt
     1141 gcggaagtaa aagcaactag tagatcatct aaattaggat caatcgttag catggaagta
     1201 aatgtatgat cgaataaagt taaatcttga tggatctcgt cactaaacag aatcacttga
     1261 tgttttaaac acaattcgct taactgtttc aattcttctt tgctccaaac acgccctcca
     1321 ggattgtgag gattgcataa aatcattgct tttacattgt tttcttcgat ttttttctcc
     1381 atgtcagaca agtccatgat gaaatgatta tttttttcta gcaaagagct tcgaacgatg
     1441 tgacgttgat ttgtttctat aatagaggaa aatggaggat aaactgggtc atgaatcaaa
     1501 atagaatcgc ctggttttgt aaaagtttgt actgctgcag ctaaactggt taacacacca
     1561 cttgtaaaaa cgatattttc tttcgtcaac tccacagcat ggtgttggcg ttcccaatga
     1621 atgatagcct gatatagttt atcagacaga gtggcataac caaagattcc atgtttgatg
     1681 tattcactaa acgctttagc taccccaccg ggagacaaga agtccatatc tgctacccac
     1741 aaaggaagta agtcatccat ttgatatgtc tcagcaattg cgtcccattt tacactttct
     1801 gtatcttttc ttgaaatctg tttatcaaat tcgatcatct taagacctcc agtcattttc
     1861 gtcgcttctt tttttgagct atttacagta taatgataaa tgcgaagaga gggaagtaga
     1921 tgacttataa aattggacaa ataattgaag gaaaagtgac agggatacag ccttatggcg
     1981 cattcgtttc tcttgataac gagacgcaag gacttattca tgtttcagaa gtacaatccg
     2041 gttatacgaa aaatatccat tcattgttaa aagtcggaca gccagtaaca gttcagatca
     2101 tcgacattga tgaatattcc aaaaaaatca gtttatcttt aagaacgtta gaaaaaacaa
     2161 atccagctgt tccatatcgc agaaaaaggt atttcacgaa taaaaatcgt aagatcggat
     2221 ttaccactat tgcggagcag ttacctattt ggatagaaga agcattatct gaactagaac
     2281 ggaaagaaaa taagaaataa aaagtgtatt ttttctgaaa atgtgctaga atttttatgt
     2341 aagccataga acaagttgat cgaggtggaa attttggcaa gcaaaagagt tgctggtaca
     2401 attatgttgc atttggagga tggtactaaa aaatttttgg tccattcaat tgatgacaag
     2461 ttggaatttg cgttagcgga actttctgaa ggaaagactg gtttagcgaa tatacttaat
     2521 ttcttaaaag aaatagttca tatcgatgtt agcaagatta gtttgatgga attgacaaat
     2581 acacatatta aagaggaaaa tgttccgttg tttgtctttg aaactgaaca aaagaatcaa
     2641 agagaagaat taggcgaagg atatatttgg gaagaaccag gtcaaatgcg ctctgttctt
     2701 ggaacttacg aagtagaggg agtgcctttc ttctaaaata cagattaagc gaacaaagaa
     2761 gtttgaattt ttttcaaact tcttttttta ttgtttggtc caaaaaatac aatgaaatgt
     2821 tctgctaaag atagtttttt agtgcatttt attttcaaaa tatgttgaaa acggttgtat
     2881 aacgaataat aataattgat cgaaaaatca tttcacaaag taatgcttaa tttatagttg
     2941 accaaggaag aaatacatga taaatttaac agcgttgatt ctgaagtgga tgttatatca
     3001 catttctaaa aaatttaaaa aatagttatt gacatgttac taacgacatg ataagataac
     3061 tgagtcgtta aaaaagaacc acttgcgaag cttgattcta tcgaaaaaaa cttcaaaaaa
     3121 gttcttgaca aataacaaac gataagttat gataaatgag ttgctgaaag atttcgaaaa
     3181 acgaaatcga aaaaaacttt taaaaaagag ttgacaatcg cttgtctctc tgatatgata
     3241 taaaagttgc tacggcagcc aaaaacgaat cgggaaacac tcccgatgaa gtagaccttt
     3301 gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg tcttgattca attcaagaca
     3361 acaaac
//
LOCUS       DAPBCS010000115         3364 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPBCS010000115 DAPBCS010000000
VERSION     DAPBCS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.121"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1116)
                     /locus_tag="RD521_002857"
     CDS             complement(<1..1116)
                     /locus_tag="RD521_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HDT7772508.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKT"
     gene            complement(1258..1482)
                     /locus_tag="RD521_002858"
     CDS             complement(1258..1482)
                     /locus_tag="RD521_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDT7772509.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            1733..3319
                     /locus_tag="RD521_002859"
     CDS             1733..3319
                     /locus_tag="RD521_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDT7772510.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
BASE COUNT          976 a          679 c          625 g         1084 t
ORIGIN      
        1 agttttccca acaataccta atgtttctcc ttcttggatc gcaaacttga tttgttctaa
       61 tgctggtgat tcttctttcg gataattaaa agaggaaata gctacattca gctcgccttt
      121 tgccggggtc tgcactgcac ccagttgttc cacgatatgc gtcttttcat gcaaaagttc
      181 attgacgcga tcataagaag catttcctct ttccaatacg ttgaacaatc gaccaatcgc
      241 aaacatcggc caaacaagca taccgatata actaataaat gagaccatct gcccgatcga
      301 gatcgtgcct tccaagacat actttccacc taaaataatt gttgcaacat aagacagacc
      361 gataatcaag gtaatgaatg gatcaaataa ggagtctaaa aaattgactc gtttgttttt
      421 aataattgca tcttcaatct tttcttcaaa atccgctaga tcttcttttt cttgtccaaa
      481 tgttttaatg actttgatcc cagtgatact ttcttgtgtc ttatcattga tactagaaaa
      541 agcttcttgc gaatcacgaa aagcatcatg gagttttgac cccagcactc gtgaagtgac
      601 ggccagcaaa ggcagaggaa tcagcgcaat cagtgttaag cgccaatcaa taaacaaaat
      661 catcgcaatg atcgttgttc cgcccgtaat cactgaatct gcaaaggtca agataccagc
      721 tcctgctaca ttctggatcg cattcaaatc attcgttgcg tgagccatca aatccccagt
      781 ccgatatttt tggtaaaaga gattgtccat ttctgtaaaa tgtttgaata actgctgccg
      841 aagttctttc tccaatcgag ctgcacttcc ccaaatattc gtccgccaga tataacgaaa
      901 aagatattgt aaaaaagcag cgataatcaa taggaagatc cacccgaaaa tgatctttat
      961 tgataaattt ttatcggcga tctcatcaat gatgatccca atcacttttg gaggaactaa
     1021 ctgtaccaag gccactgcga atagcgagaa aaccccaatt aaataatgtt tcttttcctg
     1081 acgaaaaaac cagcctaatt ttctaaaaat cgacatttta ctcctccttt gtctttatca
     1141 agagcctagg attgtctgtg cttgtctttt ttgctgaata atctgaatag tctaacaaat
     1201 tacgaaataa aaaacggact gaaaaaatca gcccgtaaaa agtcgttggg tcagaaccta
     1261 ttttttccct tggttcttca tttgctgcat catctggcga attttcttct cagatggttt
     1321 ttgccccatt gacatcatca ttgatcgcaa catgtcctca ttaacgggag gatttttttc
     1381 taaatagtct ttcatatatt tacgcgctag gaaaaagcca cctactgcgc cgattagcaa
     1441 agcaataata gcaataagta cgacaatacc tgttgataac atataaatct ctcctttcta
     1501 tttccgaatt gctctcaatg acttatttta ctaaagttta ttgaaaaaag aaaggcgtat
     1561 tctttagttt catgcaaaaa atagtggttt ttttagagga gaacaaaatt tctttcgtca
     1621 tctagcaaga cgcgccatga aaaaaaattg ggcgtaccga atctttttct ttaatttttt
     1681 ctttttgagg tgtagaatgt aatcgtagat aaataggaaa ggtggttttt tcttgaataa
     1741 tcaatccaat aaacaatttc ataagcatcg acttattgaa catacaaatg gtttatcttt
     1801 agctgaagta aatggaacag ttgatgtacc aaaggggaaa agttttttta aaacacttct
     1861 tgcttactct ggacctggtg cgttagtagc agttgggtat atggacccgg gtaactggtc
     1921 tacttcgatc accggaggac aaaatttcca atatttattg atgtcagtta ttttgatgtc
     1981 tagtctgatt gctatgcttt tacagtatat ggcagcaaaa ttaggaatcg tgagccaaat
     2041 ggatttggca caggcaatcc gagcacgcac aagtaagaca ttaggtgtca ttttatggat
     2101 cttgactgaa ttagccatta tggcaacaga tattgcagaa gtcattggtg cagcaatcgc
     2161 cctctactta ctttttgata ttccattggt gattgccgtc tttattactg tattagatgt
     2221 tttcgttctc ttactactga caaaagtcgg gtttcgtaag atcgaagcac tggttgtctg
     2281 cttaattttc gtcattttat tcgtttttgt ctatcaagtt ggcttgtcta acccagattg
     2341 gcacagttta tttgctggct ttatccctaa cagtcataca ttctcagact caccggtcat
     2401 cggcggcgaa actccgctga ctggagcttt agggattatt ggtgcaacag tcatgccgca
     2461 taacttatat cttcattcag cagtttcaca aactcggaag atcgatcacc aaaacgaaga
     2521 agatgtagca caagctgtac gtttttctac ttgggattct aatatccagt tgtcaatggc
     2581 ttttctagtc aatgcactgc tattgatcat gggtgtcgct gtcttcaaaa ccggttctgt
     2641 ccaagatcct tccttttttg gtctgtatga agctttatct gatccatcag ctatgagcaa
     2701 tggtattttg attgctgtag caaaatctgg tatcttgtcc actttatttg ctgtagcact
     2761 tcttgcttct ggccagaact ctactattac tggtacgttg accggtcaag tcatcatgga
     2821 aggattcatc catatgcgta tcccgatttg gctaagaaga ctggtcacac gattgctttc
     2881 tgtgattcct gtattgattt gtgttttgtt tacaagtaca aaaggaacga tcgaagaaca
     2941 tgccgctttg aataacttaa tgaatgaatc ccaggtgttc ttggcttttg cgttgccgtt
     3001 ttcgatgatt cctttactga tgatgaccaa cagccaagcg gagatgggta gccgatttaa
     3061 aaattcattc atcgtaaaac ttcttggctg gttctctgtg attagtctaa cttacttgaa
     3121 cttacgagga ttaccaggac agatcgaaag tttcttcggc gagcaagcca cttccgctca
     3181 actagcattg gctgatcaca tcgcttatgt aatcattgtt cttgtccttc tcctgttggc
     3241 atggacgatt ggtgatttat ataaaggaaa caaaaaatat gaacagcttt tgcaggaaac
     3301 ggtagaagaa atcgaataaa aactgatttt tccacacaaa gacccttgat agttaggtta
     3361 aaaa
//
LOCUS       DAPBCS010000116         3048 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPBCS010000116 DAPBCS010000000
VERSION     DAPBCS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.122"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            150..353
                     /locus_tag="RD521_002860"
     CDS             150..353
                     /locus_tag="RD521_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HDT7772511.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(494..979)
                     /gene="ssb"
                     /locus_tag="RD521_002861"
     CDS             complement(494..979)
                     /gene="ssb"
                     /locus_tag="RD521_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDT7772512.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(982..1881)
                     /locus_tag="RD521_002862"
     CDS             complement(982..1881)
                     /locus_tag="RD521_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDT7772513.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(1894..2487)
                     /locus_tag="RD521_002863"
     CDS             complement(1894..2487)
                     /locus_tag="RD521_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDT7772514.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(2563..2712)
                     /locus_tag="RD521_002864"
     CDS             complement(2563..2712)
                     /locus_tag="RD521_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772515.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(2728..>3048)
                     /locus_tag="RD521_002865"
     CDS             complement(2728..>3048)
                     /locus_tag="RD521_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HDT7772516.1"
                     /translation="QEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKK
                     LTATQLKEIIQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTR
                     RTTK"
BASE COUNT          800 a          592 c          506 g         1150 t
ORIGIN      
        1 taaagtaaag cgaaccaata atggttgtgt ttttattact cggattttat caagcaaacc
       61 aacatatttg ttcatcacga tcacctcgct aaaattatag caaaaacagc taaaattgag
      121 aatttttatt cgattttaga tatgctataa tgatcaagac agtaaagttt gtgacggtgg
      181 ctatccgatt gaaagtgagg tggtgcgtat gtgcgtactt ccaaaatctt acagaaagga
      241 gaagcctttt gcctgttgaa acggcgctag gattaatgat tagtttcggt tctttgatcg
      301 caacaatcat ttttggaatc ttagcagttg taaacaacaa tcaaaaaaaa taaccgtcag
      361 ctttagccgg tcgacgatta tttttcaaaa ataatattaa gctggtcacc gtcttaaacg
      421 gtgctgttag gggagttgtt agcgcaactc ctcttttcgt atccattata acaactttat
      481 tgatctttta caatcagaat ggtaaatcat catcactaat atcgattgat tgtccgccaa
      541 atggattttg tgaagcgttc aatccattgt tgttaggcgt agctacatcc cgttgttcaa
      601 aggtggtagg gcttggactg ctggacgatt gatcagcgtg cgcacggctt tctgtcgctg
      661 ctttactttc taatagttgg aaattatcgg ccacaacttc tgtcacatac acacgttgtc
      721 cttgttggtt atcgtaggtt cgggtttgaa tccgtccaac aacgccgatc aaactgccct
      781 ttttagctaa tgtcgccatc gtttctgctg gtttgcgcca aatcacacag ttaataaaat
      841 cagcttctcg attgccgttt tgattcgtga aatttcggtt gacagctagg gtaaaagtcg
      901 ctactgcagt accactcgcg gtataacgta aatctggatc tttggttaag cgtcctacta
      961 aagttacatt attgatcatc tttatctctc catttcttga ttcatttgtt gtgatctttt
     1021 aaactgccgt tttctttctt gattttcaat tttgatcaca cttttcatta ggtgatcaat
     1081 ttcgttgact gtatctgtaa atgaaatgga attcgcaaac gtgttaactg agcgatattt
     1141 ttcccactgc ttttccaaat agtcatttcc tctgatctta tccatttctc tttcataata
     1201 aaaggacaac ggttttgtat ccccacgttt tgtaaatgta tttcgaacac ttttgtataa
     1261 ctctttcgta tcgatatttt ctagtttcgg aatggtaaat aagtcgtagt aatcttttgc
     1321 tctcgaatta tctgtgccaa agctgaacgt agcacttagc ttttctgcaa taatttgttc
     1381 tgttggataa gctggaatct ccactttttt atcttcaaac attaattggt gatagtgtgt
     1441 atctacttca ggataaatag aatctcctgt tgtcacgtcc attttaaacg gtacacgtac
     1501 tttctctaaa ttagccacca atttaatttg aaatccagga taaaaatcgg cttctcttgt
     1561 ttccttcatt ccttgaatag agaaaacaat gccttctttt gtgggttttc tagttaaatc
     1621 attaaaaatg ctcttcaatt tttcttcagt taatttgcta tttctaaatg tcgtatcgat
     1681 atcaattgtt gtccttctat cgattccata ttttgatcct aataaaaagc cgcctttcaa
     1741 aatgaagtca tttttgtact ttgattcaga aatttgttca attagacgat ccatcataaa
     1801 atgccgttgc actaactgtg catttaatcc tgtactcttt gctaaatttc ttgtttttgc
     1861 tgcaaattgt gtcggtgtca tctttttcct ccatcaatat aagctttgca tgtggtattt
     1921 catttctttt tctacatgta gttttgtcat gtaatctctt aatttacttg gatctctcaa
     1981 tgggtcttcc atgtactctt ttaatgcttc taatttcatt tcatattcaa attcatatct
     2041 cggattccaa atatcacaaa gtgtacgctc tttatcatac atagtaatag gatttccttg
     2101 aaaggaagtt ccctctactt ttcctagttc ataaacttct ttcttcacga aatgaaattg
     2161 taactgctgc tcttttgtaa ttgaacgatc aacgttatag ccatggggaa aagttaagtg
     2221 aatgaatttc ggcatattcg tagaaagctg gtaaatatct aaagtcgttt catgagagaa
     2281 gattcccctg gaaaatcgtt gctgaagcca aaataaatca tctattccat gaccatcctc
     2341 tacataataa ccacgatcaa ctttgatcaa ttttccttga ttcaccaatc tttgtacagt
     2401 ggttgctgcg attccaaatt tcctggcttc cttcatagat aaatttccat cgcgaatagc
     2461 caacaattct ttcagtttat cttgcattta cttcccctcc cttcgttaca aatacactac
     2521 atcaaatata tcatagtagt ttatttgtaa caagattaac gtctatcgat ctaattcgat
     2581 ttctttttga atgcgctgct ggttctttaa ttgttgcagt cttcttttat ttgcacgtaa
     2641 tttctcttgt tctaaacgaa tttttgcttt ttgattttcg atttcttgtt gtttgtgtgt
     2701 tgtatttcgc ataggataac cccttcatta ttttgtagtg cggcgtgttc ttttcttata
     2761 gttcgttttt ttcttttcag aaaattcaaa gcctgttgta aagtcttctt ttaaaacgat
     2821 cgttgcttca aacgaacctt tctgaccttt tagtcctctg actgattggc ttgttcggcc
     2881 gttttgaatg atctctttca gttgtgtggc tgtgagtttc tttttggcaa tcgttgtcca
     2941 taatttaaaa tcacaggctt cagacgtaca tgccgccaca ttttgataaa gcaagacttc
     3001 ttgttcccga cattttggac aaacacctaa atgacgcgtt gcttcctg
//
LOCUS       DAPBCS010000117         3011 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPBCS010000117 DAPBCS010000000
VERSION     DAPBCS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.123"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            177..359
                     /locus_tag="RD521_002866"
     CDS             177..359
                     /locus_tag="RD521_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDT7772517.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="RD521_002867"
     CDS             462..746
                     /locus_tag="RD521_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772518.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="RD521_002868"
     CDS             762..998
                     /locus_tag="RD521_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772519.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="RD521_002869"
     CDS             1020..1274
                     /locus_tag="RD521_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772520.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="RD521_002870"
     CDS             1356..1541
                     /locus_tag="RD521_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDT7772521.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="RD521_002871"
     CDS             1608..2003
                     /locus_tag="RD521_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7772522.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="RD521_002872"
     CDS             1993..2370
                     /locus_tag="RD521_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772523.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="RD521_002873"
     CDS             2392..2766
                     /locus_tag="RD521_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772524.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1059 a          507 c          523 g          922 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcatttaaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc t
//
LOCUS       DAPBCS010000118         2926 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPBCS010000118 DAPBCS010000000
VERSION     DAPBCS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.126"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            253..1221
                     /locus_tag="RD521_002874"
     CDS             253..1221
                     /locus_tag="RD521_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDT7772525.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1214..2245
                     /gene="vanA"
                     /locus_tag="RD521_002875"
     CDS             1214..2245
                     /gene="vanA"
                     /locus_tag="RD521_002875"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDT7772526.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2251..2859
                     /locus_tag="RD521_002876"
     CDS             2251..2859
                     /locus_tag="RD521_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDT7772527.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          870 a          557 c          713 g          786 t
ORIGIN      
        1 cagcgatgcc agacttggtt gataaaagga ggtcctaaga gatgtatata attttttagg
       61 aaaatctcaa ggttatcttt actttttctt aggaaattaa caatttaata ttaagaaacg
      121 gctcgttctt acacggtaga cttaataccg taagaacgag ccgttttcgt tcttcagaga
      181 aagatttgac aagattacca ttggcatccc cgttttattt ggtgcctttc acagaaaggg
      241 ttggtcttaa ttatgaataa catcggcatt actgtttatg gatgtgagca ggatgaggca
      301 gatgcattcc atgctctttc gcctcgcttt ggcgttatgg caacgataat taacgccaac
      361 gtgtcggaat ccaacgccaa atccgcgcct ttcaatcaat gtatcagtgt gggacataaa
      421 tcagagattt ccgcctctat tcttcttgcg ctgaagagag ccggtgtgaa atatatttct
      481 acccgaagca tcggctgcaa tcatatagat acaactgctg ctaagagaat gggcatcact
      541 gtcgacaatg tggcgtactc gccggatagc gttgccgatt atactatgat gctaattctt
      601 atggcagtac gcaacgtaaa atcgattgtg cgctctgtgg aaaaacatga tttcaggttg
      661 gacagcgacc gtggcaaggt actcagcgac atgacagttg gtgtggtggg aacgggccag
      721 ataggcaaag cggttattga gcggctgcga ggatttggat gtaaagtgtt ggcttatagt
      781 cgcagccgaa gtatagaggt aaactatgta ccgtttgatg agttgctgca aaatagcgat
      841 atcgttacgc ttcatgtgcc gctcaatacg gatacgcact atattatcag ccacgaacaa
      901 atacagagaa tgaagcaagg agcatttctt atcaatactg ggcgcggtcc acttgtagat
      961 acctatgagt tggttaaagc attagaaaac gggaaactgg gcggtgccgc attggatgta
     1021 ttggaaggag aggaagagtt tttctactct gattgcaccc aaaaaccaat tgataatcaa
     1081 tttttactta aacttcaaag aatgcctaac gtgataatca caccgcatac ggcctattat
     1141 accgagcaag cgttgcgtga taccgttgaa aaaaccatta aaaactgttt ggattttgaa
     1201 aggagacagg agcatgaata gaataaaagt tgcaatactg tttgggggtt gctcagagga
     1261 gcatgacgta tcggtaaaat ctgcaataga gatagccgct aacattaata aagaaaaata
     1321 cgagccgtta tacattggaa ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg
     1381 cgcggaatgg gaaaacgaca attgctattc agctgtactc tcgccggata aaaaaatgca
     1441 cggattactt gttaaaaaga accatgaata tgaaatcaac catgttgatg tagcattttc
     1501 agctttgcat ggcaagtcag gtgaagatgg atccatacaa ggtctgtttg aattgtccgg
     1561 tatccctttt gtaggctgcg atattcaaag ctcagcaatt tgtatggaca aatcgttgac
     1621 atacatcgtt gcgaaaaatg ctgggatagc tactcccgcc ttttgggtta ttaataaaga
     1681 tgataggccg gtggcagcta cgtttaccta tcctgttttt gttaagccgg cgcgttcagg
     1741 ctcatccttc ggtgtgaaaa aagtcaatag cgcggacgaa ttggactacg caattgaatc
     1801 ggcaagacaa tatgacagca aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg
     1861 ttgtgcggta ttgggaaaca gtgccgcgtt agttgttggc gaggtggacc aaatcaggct
     1921 gcagtacgga atctttcgta ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc
     1981 agttataacc gttcccgcag acctttcagc agaggagcga ggacggatac aggaaacggc
     2041 aaaaaaaata tataaagcgc tcggctgtag aggtctagcc cgtgtggata tgtttttaca
     2101 agataacggc cgcattgtac tgaacgaagt caatactctg cccggtttca cgtcatacag
     2161 tcgttatccc cgtatgatgg ccgctgcagg tattgcactt cccgaactga ttgaccgctt
     2221 gatcgtatta gcgttaaagg ggtgataagc atggaaatag gatttacttt tttagatgaa
     2281 atagtacacg gtgttcgttg ggacgctaaa tatgccactt gggataattt caccggaaaa
     2341 ccggttgacg gttatgaagt aaatcgcatt gtagggacat acgagttggc tgaatcgctt
     2401 ttgaaggcaa aagaactggc tgctacccaa gggtacggat tgcttctatg ggacggttac
     2461 cgtcctaagc gtgctgtaaa ctgttttatg caatgggctg cacagccgga aaataacctg
     2521 acaaaggaaa gttattatcc caatattgac cgaactgaga tgatttcaaa aggatacgtg
     2581 gcttcaaaat caagccatag ccgcggcagt gccattgatc ttacgcttta tcgattagac
     2641 acgggtgagc ttgtaccaat ggggagccga tttgatttta tggatgaacg ctctcatcat
     2701 gcggcaaatg gaatatcatg caatgaagcg caaaatcgca gacgtttgcg ctccatcatg
     2761 gaaaacagtg ggtttgaagc atatagcctc gaatggtggc actatgtatt aagagacgaa
     2821 ccatacccca atagctattt tgatttcccc gttaaataaa cttttaaccg ttgcacggac
     2881 aaactatata agcggttctg ttgcaaagtt ttaaataaag aataaa
//
LOCUS       DAPBCS010000119         2919 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPBCS010000119 DAPBCS010000000
VERSION     DAPBCS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.127"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(162..1535)
                     /gene="tet(L)"
                     /locus_tag="RD521_002877"
     CDS             complement(162..1535)
                     /gene="tet(L)"
                     /locus_tag="RD521_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7772528.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            complement(1633..2787)
                     /locus_tag="RD521_002878"
     CDS             complement(1633..2787)
                     /locus_tag="RD521_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7772529.1"
                     /translation="MEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSIN
                     GELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQ
                     REMLLDALLEISDSDPLLQYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKE
                     PTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSF
                     QNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKA
                     GTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEY
                     RSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT          990 a          590 c          449 g          890 t
ORIGIN      
        1 gtgtaacgga caaaaccact caaaataaaa aagatacaag agaggtctct cgtatctttt
       61 attcagcaat cgcgcccttt aatggaacaa aaagatttga attcctgtta taaaaaaagg
      121 atcaattttg aactctctcc caaagttgat cccttaacga tttagaaatc cctttgagaa
      181 tgtttatata cattcaaggt aaccagccaa ctaatgacaa tgattcctga aaaaagtaat
      241 aacaaattac tatacagata agttgactga tcaacttcca taggtaacaa cctttgatca
      301 agtaagggta tggataataa accacctaca attgcaatac ctgttccctc tgataaaaag
      361 ctggtaaagt taagcaaact cattccagca ccagcttcct gctgtttcaa gctacttgaa
      421 acaattgttg atataactgt tttggtgaac gaaagcccac ctaaaacaaa tacgattata
      481 attgtcatga accatgatgt tgtttctaaa agaaaggaag cagttaaaaa gctaacagaa
      541 agaaatgtaa ctccgatgtt taacacgtat aaaggacctc ttctatcaac aagtatccca
      601 ccaatgtagc cgaaaataat gacactcatt gttccaggga aaataattac acttccgatt
      661 tcggcagtac ttagctggtg aacatctttc atcatataag gaaccataga gacaaaccct
      721 gctactgttc caaatataat tcccccacaa agaactccaa tcataaaagg tatatttttc
      781 cctaatccgg gatcaacaaa aggatctgtt actttcctga tatgttttac aaatatcagg
      841 aatgacagca cgctaacgat aagaaaagaa atgctatatg atgttgtaaa caacataaaa
      901 aatacaatgc ctacagacat tagtataatt cctttgatat caaaatgacc ttttatcctt
      961 acttctttct ttaataattt cataagaaac ggaacagtga taattgttat cataggaatg
     1021 agtagaagat aggaccaatg aatataatgg gctatcattc caccaatcgc tggaccgact
     1081 ccttctccca tggctactat cgatccaata agaccaaatg ctttacccct attttccttt
     1141 ggaatatagc gcgcaactac aaccattacg agtgctggaa atgcagctgc accagcccct
     1201 tgaataaaac gagccataat aagtaaggaa aagaaagaat ggccaacaaa cccaattacc
     1261 gacccgaaac aatttattat aattccaaat aggagtaacc ttttgatgcc taattgatca
     1321 gatagctttc catatacagc tgttccaatg gaaaaggtta acataaaggc tgtgttcacc
     1381 cagtttgtac tcgcaggtgg tttattaaaa tcatttgcaa tatcaggtaa tgagacgttc
     1441 aaaaccattt catttaatac gctaaaaaaa gataaaacgc aaagccaaat taaaatttgg
     1501 ttgtatttaa ccctgttaca ttcattacac ttcataatta attcctcctt tacactttaa
     1561 ttcaaatctt tattaaaaaa tatttcatct tatttaacaa gaaaccatat ttatataaca
     1621 acataaaata cactaagtta ttttattgaa catatatcgt actttatcta tccgactatt
     1681 tggacgacgg ggctggcaaa caggttcacc ggtagtaaca tggtaccctt ttaactctgt
     1741 taaacaaaca ctacgtccat ttgtaaagaa agttaaatca ctacgatatt cttgaataca
     1801 ccgagcaggg atttctccac taagaatgac ctcattattt ttcaattgag tgtctacgat
     1861 gttcgcacaa tatttaggag catcgttgta tgctcgtgaa agatattcct gtggcgcata
     1921 aattttaaaa ctaagatatg gctctaacaa ttctgttcca gcttttttta agacttgttc
     1981 caatacaata ggagcaagca tccgaaaatc tgctggggta ctaacagggc tatagtataa
     2041 gccatactta aaacagattt tacaatccgt cacattccaa ccatataatc cttgttcgca
     2101 accatagcgt atcccttcca taactgcatt ttgaaatgat tgatttaagt atccaagaga
     2161 aaccgagctc tcatactgca ttccacttcc caacggaagc ggtgatacag ataaaccaat
     2221 ggaagcccag aaaggatttg gcggcacttc gatgtgaatg gtatattctg cattttttaa
     2281 cggtctctcc atataaatga ctgtaggctc ttttagttct atctccacat gatacttttc
     2341 ttgcaacagt gcactaatca cttccatttg tactttccct aagaaagaaa gtataatttc
     2401 atgtgtcgta gaatccacat aatattgtag aagcgggtca ctgtcggaga tttctaaaag
     2461 tgcatcaagt aacatttccc tttgttgagg tttgctcggt tcaacagttg tttgcagcag
     2521 agggagcgga ttttcaattc tctctctctg tggcaatagc tttgtatctc caagaacact
     2581 atttagcttc aaaaactcat tttgcaaaat aacaatttcc ccggaataag ccttatcaat
     2641 tttacataat tcaccattta ttgaagtata catttctgta atttttattt tttccttttc
     2701 cgatattcta accgaatccc gcaaatgcag gattccgcca taaaggcgta catatgcaag
     2761 acgttgtctt tcttccgaat attcaatttt gaagacattt ccgcaaagtt cagacttctt
     2821 tctgtatgtt gatgaataaa atttattcgt tatcacttct ataagctgct caatccctat
     2881 gttgcttttt gcgcttccat gataaacagg gtacaagga
//
LOCUS       DAPBCS010000120         2719 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPBCS010000120 DAPBCS010000000
VERSION     DAPBCS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.128"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="RD521_002879"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="RD521_002879"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDT7772530.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DAPBCS010000121         2492 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPBCS010000121 DAPBCS010000000
VERSION     DAPBCS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.130"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            669..1358
                     /locus_tag="RD521_002880"
     CDS             669..1358
                     /locus_tag="RD521_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDT7772531.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1345..>2492
                     /locus_tag="RD521_002881"
     CDS             1345..>2492
                     /locus_tag="RD521_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDT7772532.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHL"
BASE COUNT          777 a          431 c          478 g          806 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcagg agtgtttccc gattcgtttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gtaattgtca actctttttt aaaagttttt tcgatttcgt
      181 ttttcgaaat cttccagcaa ctcatttatc ataacttatc gtttgttatt tgtcaagaac
      241 ttttttgaag tttttttcga tagaatcaag cttcgcaagt ggttcttttt taacgactca
      301 gttatcttat catgtcattt ccaacatgtc aacaacttat tttttattat ttaataagtt
      361 attggtgttc ttttgaacaa ataataatat atcaacatca catgtgaaag tcaataagaa
      421 aatgcttatt tatcaacatt tctagcttac atgatcattg gttagttctg gtcctttttc
      481 gttcgtcttt ataactccga gagacgtaaa atccttttgt tttcagttta atgttcatgt
      541 tcttgtctta ttcacaccaa ctagatgtct caccttctaa aattttcctt aacaaagtgc
      601 tgcttttatc tatttctgct tttttttgct agaataatag taacaatatg actctggagg
      661 atttatttat gaaaattttg attgctgacg atgataaaga aatcgttgaa ttattaagta
      721 tctatgttca caatgagggg tatgaagcag taaaggctta tgatgggaaa gaagcattat
      781 ctaaaatcag aacaatccct gatattgaat tacttatttt agatatcatg atgcctgaga
      841 tggatgggat gcaagtagtg aaagagctac gaaaagaatc ccaaatacca atcattatgc
      901 tgacagcaaa aactactgat atggataaga tcaaaggact ggtcgctggt gcagatgatt
      961 atgtgaccaa gccatttaat ccgttagaag taatggctag agtaaagtct ttattacggc
     1021 gtacaaaaat gcagattgcc ccagatcagc ctgatgtatt agaagtaaat gcattaatca
     1081 tcaataaaga ctctcatgaa gttaaaacaa tcgatgggaa agaaatccag ttaacagctt
     1141 tggaattcgg tatcctctac ttgcttgcta gtcatcctaa tcgagtattt agcgcggatg
     1201 aaatttttga gcgtgtttgg aaacaagaaa gtattgtctc tgctaaaact gtcatggttc
     1261 acgttagtca tttaagagat aagattgaag aagcaactgg tggtgaaaaa gtcattcaaa
     1321 ccgtttgggg agtgggctat aaaattgacg caagatagta aacgagcaac tgaaaaacgc
     1381 cgtcggatca atttgacttc aaaagaaatc agcgaattgt tagcggaagg aatcatcacg
     1441 attatccttt tgcttctatt aaatgtttct attctggttg ttatcagttc ggtgatcaac
     1501 agttcacctt ctttgacgaa tgcgatttgg gattcaaaga atatttttgc agaacgattg
     1561 aacactgatt tgttctggaa cggccggaat tttattattc cctttttctt tctattagat
     1621 attggtgttt tgtattggcg cttgattcga agatatcgcc aaatgcaatt gcggcatatc
     1681 atcagcgaac ttcactacat cgcaaacgga aattatgatc atcgtatccc ttttgaactg
     1741 agtggtgact taagccgagt ggtaacaagt atcaatggat tggtggatag taccgtagca
     1801 gcgattgaag acgaacgtaa aatcgaaaaa tcaaaagatg aattgatcac gaatgtcagt
     1861 catgatatcc gtaccccact cacgtccatc attggatacc taggactgat agaagatggc
     1921 cagtatcact cagaagaaga tcttttaaag tatacgcata ctgcttatat caaagcaaag
     1981 cagatgaaat ctttagtgga cgacttattt gaatacacta aggtacgcca gcctgctgtc
     2041 ccggtgaatt tttctgcctt tgacatgatc cagttgatcg aacagttagc agctgatttt
     2101 gagttggaag cttctaaaaa aaatatccaa atacttgttc aatcaaaagt agactccctt
     2161 atcatggatg gcgataccga aaaacttgta cgtgtcttca ataatctttt gacgaatgcg
     2221 ttgaaatacg gaaaaggagc aacgaagatc gtaatcgaag tagaacgtat cggttcagaa
     2281 gttgttgcaa cagtcaaaaa taacggagca atgatcccac aacaggcaat cgataatcta
     2341 ttcgaccgtt tttatcgtgt agaagaatct cgttcacaag caactggagg aactggactt
     2401 ggtttagcaa tcgctcaaag cattgtggct ttgcacggcg gatacattta tgcaaaatca
     2461 gataagcaat ggacttcttt catcatgcac ct
//
LOCUS       DAPBCS010000122         2466 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPBCS010000122 DAPBCS010000000
VERSION     DAPBCS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.131"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            80..>2466
                     /gene="esp"
                     /locus_tag="RD521_002882"
     CDS             80..>2466
                     /gene="esp"
                     /locus_tag="RD521_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDT7772533.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
BASE COUNT          893 a          385 c          506 g          682 t
ORIGIN      
        1 ctattctgtg aataatacgt atcagattta tcttttattc ttactaggaa agtaattatt
       61 aagggagttg atttgaataa tggttagcaa gaataataag agagtatttc tagaaaaaac
      121 aaagaaacga gtattgaagt acagtatcaa aaaattaagt gttggggttg catctgtttt
      181 gattggtgtt ggtcttgtct tgggaacaac tgaactagtt aaagcacaag atgaaatttc
      241 tccaagcagc ccacttgaaa cagcgataag ctccgtacaa gtaggtgaca aagtagcttc
      301 ggggaatact tttcaggaga atcctggtta tacgaaaaat tataactttt cggatttaca
      361 atttagtcct caagaactaa ctggagacac actaaaaggc aatacaattg gatttgaagt
      421 ttacggaaaa cataatattg cagcatcaac taaaaactgg gaaatccgtc ttcaattaga
      481 tgaacgatta gctaaatatg tagaaaaaat tcaggtcgat ccaaagaaag gaattggatc
      541 ttctagacgg acctttgtaa gaattaatga ttcgctgggt agacctacaa atatttggaa
      601 ggtcaattat attcgtgcaa gtgatggatt atttgctggg gcagaaacaa ccgatacgca
      661 aaccgctcct aatggggtaa ttacgtttga aaagagccta gatgaaattt ttaaagagat
      721 aggcatagac aatctcaaga ccgatcgttt aatgtatcga atctacttgg taagtcatca
      781 agacgatgat aaaattgtac cgggtattga tagtacgggc tactttttaa cagattcaga
      841 tgatttctat aatagcttag atgtatctga aaataatcct gaccagttta aacatggatc
      901 agtaagtgca aaatatgaga aacctaatac tcaaacaaaa gatggttcag gatccacagg
      961 tgctaatgga gctataatct tagatcataa gttaacgaaa aattataatt tctcatattc
     1021 atcttccgca aagggcacgc cttggtatgc taattacaaa attgatgaac gattagttcc
     1081 atatgtagcg ggtatacaga tgcacatggt tcaggctgac aaagtaacat atgatgtttc
     1141 gtttgaatcc ggaacaaaag tagctgattt agcaatcgag cgacgggaag gtcatgaaaa
     1201 ttatggcata ggttcaataa ctaataatga tttaactaaa cttatcgatt ttgctaatgc
     1261 aagtccacgt ccagtcgtta ttcgatatgt tttacaattg actaagccat tagatgaaat
     1321 cttagaagat atgaaagcaa cagcacaagt tgaagaaaat aaaccatttg gtgaagattt
     1381 catctttgat tcttggttgt cggatacgaa taaaaaatta atccagaaca cttatggaac
     1441 aggttattat tatttgcaag atattgatgg tgatggaaac cctgacgata aagaagagag
     1501 cggagacacg aatccatata tcgggaaacc tgaattagaa gaagtatatg atgttgacac
     1561 aacagttaag gggaaagtat tcatccacga gttagcggga acaggtcaca aagcccaact
     1621 tgttgataaa gaaggtactg tattagcaga aaaaactatc gctccaaatg aaaaagatgg
     1681 ggctccaatt tcagatactg tagaatttga atttacgggt gtagattcaa gtaaattaat
     1741 cgcgaaagat gaattaaaaa tccaaatcgt ttctccaggt tttgataaac cagaagaagg
     1801 ttcaaccgtt attaaggaat caccaaaagc ggttgataaa caaaccgtgg tagttggatt
     1861 taaaccagat gctaaagaat caattcggaa taataaaaac ttacctgaag atgcagagta
     1921 ttcatggaaa acagagcctg atacttctaa cgttactgat agtacgaaag gtattgtaac
     1981 tgttaagatc ggaaatcgaa ctttcgacgt ggatgtagag tttgctgtaa aagcttctca
     2041 agctatggaa aatgatgcaa catacgtacc tataacaaca actccagaaa cgacagttca
     2101 aagtggaaaa cctacatttg ataaaccaga tgttccttta gctaaagatg ccttttcaat
     2161 ttcagatgtc tataataagt acttcggtaa tgcaagtgtt gatgcaaaca ctggtattgt
     2221 tacattcact ccagctaaag gtgtaggaga atcggagccg attactggaa caattcctat
     2281 taaaattgtt taccaagatg gttctgtagg cacgaccgat ttagcagtaa ctgtaagtaa
     2341 agatatttat gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac
     2401 cgcaggggaa ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt
     2461 aagctt
//
LOCUS       DAPBCS010000123         2414 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPBCS010000123 DAPBCS010000000
VERSION     DAPBCS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.132"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..975
                     /locus_tag="RD521_002883"
     CDS             <1..975
                     /locus_tag="RD521_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDT7772534.1"
                     /translation="NTVKVSKKALFALMSSSSSSSKHSRLKQVIEKLHQQSVIKIQRT
                     KPNGKTEYKVISPLEITEFNDFSDAVSIKFSSSIMPLLTNLENHFTQYTFDQVVNLTS
                     KYAISFFQLFTKDFNLYEANRDNLTWSTKSLDRLKNPVFSIADLRKKFSIEKKYKAFR
                     DFERRVIKEPIAQLNKYTDFKIDYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPA
                     YLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDR
                     LSKDRGFESVVNHLSYVATHKEDYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            1010..1777
                     /locus_tag="RD521_002884"
     CDS             1010..1777
                     /locus_tag="RD521_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDT7772535.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1969..2367
                     /locus_tag="RD521_002885"
     CDS             1969..2367
                     /locus_tag="RD521_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDT7772536.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          904 a          338 c          414 g          758 t
ORIGIN      
        1 aataccgtca aagtatctaa aaaggcttta tttgcgttga tgtcttctag ttcatcttct
       61 tcaaaacatt caagattaaa acaagttatt gaaaaattgc atcagcaatc tgtcattaaa
      121 attcaaagaa ctaaacctaa cggtaagact gaatacaaag tcatctcacc gctagaaatt
      181 actgaattca atgatttttc tgatgcagta tctattaaat ttagtagttc aattatgcct
      241 ttattaacga acttagaaaa tcattttacg caatatacat ttgatcaagt agttaacttg
      301 acaagcaagt atgcgatcag tttttttcaa ttattcacta aagattttaa tctgtatgaa
      361 gcaaatagag ataacttaac atggagtaca aaatcacttg atagattaaa aaatcctgtt
      421 ttctccattg cagatttacg aaagaaattt agtattgaga aaaaatataa agcttttcga
      481 gactttgaaa gacgtgtaat taaagaaccg attgcccaac tgaataaata cactgatttt
      541 aaaattgact atgagcttat caagaagggt catacttata caaacataaa gttcttgctt
      601 acacgtacca tgtcagttcc ggtaccttat aagcaagaac aaagagaccc tgcttattta
      661 aaaggcgcag aagataaaac agagaaaatg aatcaatcgt acttgaaagc tatgaatagc
      721 aaatacacta aattactaat caaaaacttt ttgatcagtt ctgccgactt atgcgatctt
      781 aaacttatga gccgacttca gaataaagtt tatccgcttt atgatcgttt atccaaagat
      841 agaggatttg aatcagttgt caatcaccta tcttacgttg ctactcataa ggaggattac
      901 acaataaaaa atactgttaa atatctcgaa aaatcaatta cgagttattt gccttatgtg
      961 aacctttatg attaacaact gaaaaaatgc gtgaaggaag gaaaaacttt tgaaaaaaga
     1021 tgaactgaca aagtacaatg aggagttctt agcaacaatt gacatgaaaa tggatttaaa
     1081 gtcgtatgaa ctttatcaat tgctgttcag ttatatctca cccacaaagc taaagaaaac
     1141 aggcgtagta gatttacctg ttaaagcgat tattaattac atggatgttg atcaaaatct
     1201 tgaaacggaa ctaaaaacca tagttaacac tttgcaaaga caagcagttt atgaaatttc
     1261 gaatctagac aaaaaatata agacgtatac tactgtatct gctattgatc ggattcattg
     1321 ggatttagaa gagcatagtg tatctttcag tttttcagaa gatgtaattc ccttaattgt
     1381 ctcaatcaaa aagaagtatc cgttctttgt ttttgaagac ttagcatctt ttcaaaaaaa
     1441 atatagcatc gtgttataca aatatttggc gatgtattat aaaaaatatc ttatcgccaa
     1501 aaataaagga gattttgaga aagctgaaat atataagaat cccctgattg aaatagttaa
     1561 aattcgtgag attacgaaaa cagaaaatga gtataaaaca atatcgaatt ttgatagacg
     1621 tgttttaagg gaatcagttg aggaaattaa tgaaaaatca atgctgaata ttgattttgc
     1681 taaagttaga aaaggcagaa attacaattt tgctgtccag tttaatattt ctagtgagtt
     1741 tgatgaaaaa acttatagag aaaaaaaatt aggttaatgc aaaatatatc ttactcgttt
     1801 gaagatgctt cagacgtatg ctaagatata tagtgccttg ggagcaaggt attaatctcc
     1861 tttagaggtt aattagcttg tgatcgccta tcaatttttt tggtaggcga ttcttttttt
     1921 tgaaacaaaa aagcaaaaat tattttttaa ttagagaggt gcaatgcgat gaaatacttt
     1981 ttttgtgcag tattcgaatg gaaaggaaat gaatatgcag ttaaattccc agattttcca
     2041 gacttatata cacatggtga aactttagaa gatgcttttg agatggcgca agaagtattg
     2101 gaaggatact tggtgctttt agagttgcaa gaaaaagaaa ttccatcgcc tacgcctgtt
     2161 caagagttaa atgcaagaat caagcgatct gagaatgtta atttatttgt cgtaaaagct
     2221 aatacgaaag ttgctaaagc tcgaaacaag caaaattatg atcgaaaatc agtgactgtg
     2281 gaacactatt taaatgtttt agcggctgaa gagaatatta atctgtcgaa agctttgaat
     2341 gatttgttga gagaaaagtt gatttgatgt ctctcagatg atcatattaa gcaaaaacag
     2401 cttgttttag gacg
//
LOCUS       DAPBCS010000124         2406 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPBCS010000124 DAPBCS010000000
VERSION     DAPBCS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.133"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..103)
                     /locus_tag="RD521_002886"
     CDS             complement(<1..103)
                     /locus_tag="RD521_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7772537.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
     gene            complement(177..458)
                     /locus_tag="RD521_002887"
                     /pseudo
     CDS             complement(177..458)
                     /locus_tag="RD521_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            920..2302
                     /locus_tag="RD521_002888"
                     /pseudo
     CDS             920..2302
                     /locus_tag="RD521_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          875 a          412 c          400 g          719 t
ORIGIN      
        1 acaattcctg aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa
       61 taatgacgtc ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt
      121 cttttttctc aattttacac taaaatagat tttttggaaa actttgcaac agaaccgaaa
      181 tgagtatagt atgaattagt aatttggttt tcatctaagt ttagctttga tttactaaaa
      241 ttgatatctc gatacatgtt caatacatct aatacaaatc gagaatcttc tttcggaaat
      301 ggctcagaaa gatcactcca aatttcatca tcataatgat actcgtaacc ttctctcaat
      361 atttcgattt ttctttcata ataagatctg ttttggtcct caaatttagc caataactca
      421 aattgattta ctagtgacaa tctctgaaaa ctgtccatta aatgtttctt cctatcttgt
      481 tcattaacaa aaaattataa gcccttacaa cgaatattat atattaaatt taattgtagg
      541 tgaacacgaa ttttataaat atttatggtg ttaatgacaa atgatatccg actcctcttc
      601 atacgttctg gactatttgc taatagatta aaatttcagc atctatttct gaccataaat
      661 aaatgagctg ccgaaaagct gccaaatcag acttgaaaat aaaggatcaa aaacttttaa
      721 gtttctgacc ccgtaactca cacttcaaaa tcttacttaa aaaggagtct gaaagaaaaa
      781 aagagtattc aaaatttttt tctaactagc tacatcacat ctatgcattg acactcaaag
      841 aaccagaata agtcatcaga aagcaagggg gtgcggacat aatcctggac gaaaaaataa
      901 acttaggagg aatgtccgtg tgttttccca caaagagcgt gtaagggcaa ttcagttatt
      961 tttaaaatac gattgctctt atgctgcaac cattcgagaa ttagggtatc cgtcgattgg
     1021 tgcactacgg aaatggtata aagagtattt ggaatccgga gagctccatc aggagcaccg
     1081 aaaaaaatca aagtattctg aagaacaaaa acgaatagcg gtgaatcatt attttgaata
     1141 tggtcaatgt tatgcacgaa ccattcgcat gcttggttat cctaatagag agtccttgcg
     1201 gcagcggtgt gaagaacttg caccaggagc tagaaaactc cgaaaaagtg cggtaaagtt
     1261 aacacaagat caaaaagaag ctgtgttgaa aaaattctat aaaccgcaaa ctaataggaa
     1321 aaatttagcc gaatctggag gaattagtag agttactcta tatcaatgga aaaacacggt
     1381 tcttggaaag gattttccat tgtgtatgac tttaaaaaat caggaaaaac aaaaagagct
     1441 acttttaaag gaagtagaag acttacaaaa acaagtacac caattacagc tcgaaaaagc
     1501 attactcgaa ggagcggctg aactgctaaa aaaagaaaaa ggcgtcaatc ttctttgtct
     1561 atcaaatcaa gaaaaaacga tattgattga tgccttacga aatcagttca ccttgaaaga
     1621 gcttcttcaa cagctccagt ttcctaaaag cagctatttt tatcaaaaac aagcacttga
     1681 gaaaccagat aagtattaca aagaacgtca aattaattat cacgattttc aatcataact
     1741 tctgtgcgta tggttaccgt agaattcacc aagctttaaa aaatatgggt aaaaagcttt
     1801 ctgaaaaagt tgtacgtaga ttgatgactg aggaaaatct atttgttaaa ttttctagac
     1861 gaaaaaagta ttcttcgtat gctggggaaa tttctcctgc acaccccaat ttattaaatc
     1921 ggaattttaa agcaaagaag ccaaatgaaa aatggttaac agatattact gaatttagaa
     1981 ttctcgcagg gaaaatctac ttatcaccac ttgtcgattg ttacgatggt gctattgtta
     2041 gttggactat tggaacaaag ccaacttgct tcactctatg tctaaaaaga gttattcacc
     2101 agataattca gcgtgcgaag gtttctttgg acgattaaaa aacgagatgt tttatcaacg
     2161 tgactggaag aatacaacaa tcaatcagtt tataaacaaa ttggatgatt atattcattg
     2221 gtataataat caacgaatta agttatcttt aggaaggcta agccctcttc aatatcggaa
     2281 gaagcaaaga tatatttgtt aaaaaggtcc aaaaaaaata tccgcacccc cttttttttg
     2341 taaaaaacta ttgcacaata caagtagcct acggctatcg aaatttccat aactttcgag
     2401 caagaa
//
LOCUS       DAPBCS010000125         2349 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPBCS010000125 DAPBCS010000000
VERSION     DAPBCS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.134"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..543
                     /locus_tag="RD521_002889"
     CDS             <1..543
                     /locus_tag="RD521_002889"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7772538.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1016..1588)
                     /locus_tag="RD521_002890"
     CDS             complement(1016..1588)
                     /locus_tag="RD521_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772539.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1604..2209)
                     /locus_tag="RD521_002891"
     CDS             complement(1604..2209)
                     /locus_tag="RD521_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDT7772540.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          467 c          358 g          783 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaaatg tgtccaagat attgactcaa atccagtaat atcacaatag
      601 gaatgctgtt tattatatta gcagcagtgc tgatttttat gatagttatt tggttcttaa
      661 atgaaagatc taaagacaat caacaccgta gggatatgga gataaaatca atagagtcaa
      721 tagattattt atcgataata acactaaaaa agccacgttc ctaaacgtga cttacgctga
      781 acgatatgtg ttgtttggta gacgaacaaa tcgttcatta taaatatatg atggtatcct
      841 aatatagtaa gcgtggctag cataccttta attactttac cttttacagt taccatacac
      901 attttttgag cagaaatcaa gtaaataaaa aaatattata tttcacaaca tgctaataga
      961 ctcatatcta gaacgcatta agtatttacc tgtctggttc gctgagactt cttttttaac
     1021 tttcttcaat tttcttcttt attctaaaaa gcgtactgcg actgaaaccg gttatagctt
     1081 caacttcctt atacgattta cctgaagtaa gcagttcata tgcgtgcctc ttcttcggtg
     1141 tgattgttgc tttcggacgt ccttctctga aattaggatt attctttctt gcaaacattt
     1201 ttccttcttg ggtacgttcc acaatcatat cacgttccat ttctgctaca ctcaacaacg
     1261 tccgtgtcac cattcgcccc atggacgtat tttcaatcgt tcctagattc aaaaccttga
     1321 tcgttacgtt atcatcgagt aaaggttcaa tgatctctaa tgcttctctc gtgttacgag
     1381 caaatcgatc aagcttcgta acaactattt catctccagg cttcagacca gccaacagct
     1441 gattgaattt tggccgatcc cgttttgtac ccgtaaattt ttctgaaaaa atattcttct
     1501 tctctacgca gttctcaagc aactgttgaa tttgttcttt tgtactttga gaagcggttg
     1561 agacacgtgc atatccatat ttcgtcattt ggtccaccta cttttacaaa tcatctatcg
     1621 tatactcaga atacccttca tagaaatagc tgacatcgat tcctttcata tataactcgc
     1681 gatcgtcaat tttatcagtc agagcattgg aaattagata gcgtatttcc aaatcgttta
     1741 tgggacttcg ctccattgca gaaagatagt ctgctttatc gattaagttc caatctacaa
     1801 ctttttgcaa ttcttccttt aaaatgagat ccagccaaat tctagtactt cggccatttc
     1861 cttctcgaaa tgggtgagcg atattcatct ccacatattt cttgataatc tcttcaaaag
     1921 aagtttgtgg catctgatca atatgctgta acgaatgttc taaatacata actggtgcaa
     1981 atcgaaaatt tccttttgca atattgaccg atcggatttt tccggcaaaa tcgtaaatat
     2041 ccgaaaatag aaattcatga atttcagata aacctttaaa tgtaccaact tcgatttcat
     2101 ttatttttcc acaatcgtac aattctttcg ctttttgttt actgagtttt tcttcaacct
     2161 tagccaactc aacttgattg gttaatccca atttattctc taaaatcatg cgaaatctcc
     2221 ttaactttat gacataagtt tatgaaacac tctcaacccc attatatcgc gattttgttt
     2281 atgtgtcaaa aactattaag tttctgaccc ccgatagatt tttcaagtgt ctctattttg
     2341 cacagtcta
//
LOCUS       DAPBCS010000126         2335 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPBCS010000126 DAPBCS010000000
VERSION     DAPBCS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.135"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(51..1598)
                     /locus_tag="RD521_002892"
     CDS             complement(51..1598)
                     /locus_tag="RD521_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDT7772541.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="RD521_002893"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="RD521_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDT7772542.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="RD521_002894"
     CDS             complement(2043..2237)
                     /locus_tag="RD521_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772543.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          653 a          483 c          420 g          779 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga agacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttacg ttaaaaatgt tttgc
//
LOCUS       DAPBCS010000127         2270 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPBCS010000127 DAPBCS010000000
VERSION     DAPBCS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.136"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..445
                     /locus_tag="RD521_002895"
     CDS             191..445
                     /locus_tag="RD521_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7772544.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="RD521_002896"
     CDS             446..763
                     /locus_tag="RD521_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7772545.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="RD521_002897"
     CDS             1322..2257
                     /locus_tag="RD521_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772546.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DAPBCS010000128         2154 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPBCS010000128 DAPBCS010000000
VERSION     DAPBCS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.138"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..917)
                     /locus_tag="RD521_002898"
     CDS             complement(<1..917)
                     /locus_tag="RD521_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDT7772547.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVG"
     gene            1082..2095
                     /locus_tag="RD521_002899"
     CDS             1082..2095
                     /locus_tag="RD521_002899"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDT7772548.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
BASE COUNT          722 a          396 c          447 g          589 t
ORIGIN      
        1 tttccgacag caattggact gttcggaatc gtgaaaccag aagctgtcaa ttctttgaca
       61 taggagctca agaactcttc ttttaaggca tattgttttc cgttcaatgc gtagcagacc
      121 gtacgaatcg caaaattgct gtttccaaga tccactaatc caaagataga aggttcagtt
      181 tggagctctt cttggtattt ttcagctaat gtacgattta ctcgatcaat gatctcatag
      241 attttgtcat atccttcttc aggaacaatc cgaatatcta aaagtacttg catatttgca
      301 cgagagagat tgctgatcgt ggtgatgttt cgattcggga tgaaatggac agtaccatcc
      361 gttgctttca gctgaagggt acggatacca acagatacga cggttccttc aatcgttaag
      421 tttgctagct tgatgtagtc cccaacatcc atctgttgtt ccatgataat aaaaaatccg
      481 gtaataacat cactcatgaa cccttgggca ccaagaccga ttgccacacc tgcaattccg
      541 gcaccagcca aaagtgatcc gatcggcaca ccgatagcag ataaaattgc atagataaaa
      601 aagaaaccaa gggtataatg aaaaatagta tttaaaagac tatgtaaagt ctttaagcgg
      661 ctctcgttta gaatggcttt tttagaatac tgattaaaag acttatcaat cagatatttt
      721 cccacacggt gaagaatcca aaaaaggacg atcaaaaaaa tcagatataa acttttttgg
      781 ataaaggcag ccaaaatggc gtcccaatca atcccgtccc aaaaccgctg aaacgcattt
      841 actttgctga cagcttgatc tgtcaatgtc tcgccagaag agtctgtcgt accggcagtt
      901 gctaataatg aaaacataaa atcctctttt ctaaaatttg attctttctg tattgtacca
      961 agaagttttc aaaaaaggaa atagcaaaaa taactataat gaaggaaaaa agctgaaaat
     1021 aaaatgacaa ataatatgga taatgataaa atgtggagaa aataacgaag gtggtaagta
     1081 tatgcaaatc gattggaaca atctaggttt ttcttatatc aaaactcctt ggcgttttat
     1141 tgccaaatgg aaagacggac aatgggaaga aggagaattg acagaagata attacctgac
     1201 gattcatgaa ggctctccag cgcttcatta tggtcagcaa tgttttgaag gtctaaaagc
     1261 ttatcgtcga aaagacggca aggtcaatct ctttcgacct gaacaaaata gtcgacggat
     1321 gaatcaaagt gctagacgat tgctgatgcc aatggtccct gaaaagatgt tcgttgatgc
     1381 tgtcaagcaa gtggtaaaag caaatgaaga ttttgtgccg ccctatggaa caggcgctac
     1441 attatacttg cgccctttac tgattggggt cggtgaaaac attggtgtcc atccagctcc
     1501 agaatattta tttgtcgtct tctgtacgcc agtaggagcg tatttcaaag gtggactaaa
     1561 accaacgaat tttattgtat ctgattacga tcgtgctgct cctaagggga ctggtgcagc
     1621 aaaagtcggc ggaaattacg cagcgagttt acttccagga acagaagcaa aagaactgga
     1681 ttactcagat tgtgtttatc ttgatccagc cacacatacg aaaatcgaag aagtaggtgc
     1741 agcaaatttc tttggaatta caaaagacgg aaaattcatc actccgcaat cgccctctat
     1801 cttaccaagt atcacgaaat attccttgtt gcagttagct gaagaacgac taggactgca
     1861 agcaattgaa ggagatattt tcattgatga tctagatcaa tttgcagaag ctggtgcatg
     1921 tggtacagcg gctgttattt ctccaatcgg tgggatttat cacaataaca atcttcatgt
     1981 attttatagt gaaacggaag taggaccagt aacaaaacgg ttgtatgatg aattgacagg
     2041 tattcaattt ggagacatag aagcaccaga aggctggatt gaagtcgtgg aataatttca
     2101 aagtaatgag ttgaaaaaaa cgtaaaaaga aaaaatcagg aagagacttt aaaa
//
LOCUS       DAPBCS010000129         2020 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPBCS010000129 DAPBCS010000000
VERSION     DAPBCS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.139"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            100..570
                     /locus_tag="RD521_002900"
     CDS             100..570
                     /locus_tag="RD521_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDT7772549.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="RD521_002901"
     CDS             792..1895
                     /locus_tag="RD521_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDT7772550.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DAPBCS010000130         2003 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPBCS010000130 DAPBCS010000000
VERSION     DAPBCS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.140"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="RD521_002902"
     CDS             <1..378
                     /locus_tag="RD521_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDT7772551.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="RD521_002903"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="RD521_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDT7772552.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="RD521_002904"
     CDS             1859..>2003
                     /locus_tag="RD521_002904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772553.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAPBCS010000131         1963 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPBCS010000131 DAPBCS010000000
VERSION     DAPBCS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.141"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(76..1170)
                     /locus_tag="RD521_002905"
     CDS             complement(76..1170)
                     /locus_tag="RD521_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7772554.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..>1963
                     /locus_tag="RD521_002906"
     CDS             1359..>1963
                     /locus_tag="RD521_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7772555.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYP"
BASE COUNT          615 a          400 c          336 g          612 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tcc
//
LOCUS       DAPBCS010000132         1855 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPBCS010000132 DAPBCS010000000
VERSION     DAPBCS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.144"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(131..616)
                     /gene="vanZ"
                     /locus_tag="RD521_002907"
     CDS             complement(131..616)
                     /gene="vanZ"
                     /locus_tag="RD521_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDT7772556.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(769..1680)
                     /locus_tag="RD521_002908"
     CDS             complement(769..1680)
                     /locus_tag="RD521_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDT7772557.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          583 a          363 c          251 g          658 t
ORIGIN      
        1 ttactttatc accggttttc ccctcacttc acacctacgg gcgagtttcc ccacatcccg
       61 ttttccatct aatccaattc attcaatatc tttatgtgta tgaaagatca gattgcttga
      121 tttagacatc ttacacgtaa tttattctta aatgggtacg gtaaacgagc aataatacga
      181 gcaaaagtat acctacaaaa ataataactc tgtctaattt tttttgattc atatgcttat
      241 tgcttaaacc atataatttc agtccaagaa agcctccaac agtatttgta attacatctg
      301 ttatgtctgt cgctccaata gcgaagataa attgaattat ttcaaaagta agacttaaaa
      361 ccagtacaaa agcaaactta ggtaaaaatc cgatttcttt aaaattgaca ttcaaaagca
      421 agccaaatgg aataaagatt ataacattat ctatcatctc tctgaaattc ccagtagcag
      481 taaatggagt caagttaaga cttctttgat gataattaaa tactgataaa atattgtatt
      541 gtaatttgaa taacactaac cagattagtg tcactaaata taaagctagc aatcctctag
      601 ataatatttt tcccaatata atttcctcta attcttttgt ttaagttgta tcattagtat
      661 agcaatgtcc tagttaacgt gggttttaaa ttaaaaagct aacgttaccc caaaagtgct
      721 agtacaataa tgctgttcat taatttcgtt ggtttcattc cgccatcctt acctccttga
      781 attagtatgt gttgatccgg gaaacactgt cacaattaca ccgtctatat tgtttcctga
      841 tatctcataa cgaagattag tcggcacatg aatggtggta tttttagtaa caggataata
      901 aaagatctca tatttttccc catttacact aacagaaatg gttttttctt cttttaggta
      961 atccatatat tcctcgagaa cgaaattctt ttctttcata atcgcactat gtggtaaacc
     1021 aacatagcga atatgccatg gttcatattg aattcctgtt aactctgttt tgtcctctgg
     1081 ataacgtaaa atgaacccgt atttccaagc attttcttct atccactttc cttcaggggc
     1141 tcgttccatt ttcgtcaagc ttgatcctac atctagtgat aaacctgaat tatgctcact
     1201 ataacctgct ggtaaggcat actcagcccc catttcttgg taaagcacac tttgctcatc
     1261 aaagtctcga tagccactat taataataaa atgactaacg ccacccttta cagcatcatt
     1321 gaccatctct gaaaattttt gtgctatttc ttttgacata taaatattac tatcaagcaa
     1381 cccgtatcca tttattaatt cgtcatgttt agataaattc acgatatctg acttcacact
     1441 ttcttggcga acaggatatt tactattgat taatagcaga tttccttgat aaacctgttc
     1501 ttctgtaatt gttttctctt gggtattttc agaagtccca ctattttcta aatgttcttt
     1561 gggattttga tcataatttt gaaattcgac tttttcctga gaaaacagtg cttcattaac
     1621 gtagtcataa cctaagtata ttaagaataa caataacaat aaaaaaaaca acttcttcat
     1681 tttcagtctc ctccttaact atatagttgg aagtatgaaa tatttctaga atcttcccct
     1741 taaacggcac aatctcgttt gagattgtgc cgttttgccc cgtaaattac agtcaattat
     1801 ttcgccctac tggatttact attatcacca atgtagatga attttcataa gtgat
//
LOCUS       DAPBCS010000133         1740 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPBCS010000133 DAPBCS010000000
VERSION     DAPBCS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.145"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..158
                     /locus_tag="RD521_002909"
     CDS             <1..158
                     /locus_tag="RD521_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDT7772558.1"
                     /translation="SLDGHYTVFGQVIEGMDVVDAIANIETDSSDKPKEEVKIDKINI
                     LQEAKQK"
     gene            complement(273..713)
                     /gene="fabZ"
                     /locus_tag="RD521_002910"
     CDS             complement(273..713)
                     /gene="fabZ"
                     /locus_tag="RD521_002910"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDT7772559.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            933..1688
                     /gene="fabI"
                     /locus_tag="RD521_002911"
     CDS             933..1688
                     /gene="fabI"
                     /locus_tag="RD521_002911"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDT7772560.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT          572 a          323 c          317 g          528 t
ORIGIN      
        1 cttcactaga tggccattat actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg
       61 atgctattgc aaatatcgaa acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg
      121 ataaaatcaa tatactgcaa gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc
      181 atagatgaca acgtatgcta ggattcaggc gtcttctttt tacataaaaa gcaagtaagc
      241 ttccttttgt tttgctatat caggttttat agtcaagcat tttggttgac gatgaacgtg
      301 aactcgcact cacagacttt tttatcatct acatatgctg cagataacgc tgtaccgact
      361 gatcccttca ttttggtgat ttcaaaatga catttcagca catctcctgg aacaactttt
      421 tgacggaatt tggccttatt gatcccaccg atataagctc tttttcctag aaactcttct
      481 gattttagga taagtatcga acctgcttgt gccagtgttt ctagaatcaa cgcacccggc
      541 atcactggat tgtcaggaaa gtgaccttgg aaaaaatctt cattgattgt tacatttttt
      601 gtcgcaacga tcttttttcc tggttctaaa gtatctacat aatcaatata acaaatagga
      661 taacgattag gaatcaattc cattacttca gtagcagata atacgtaaga cataattaaa
      721 ctccttcttt caggcaattg ccattttcat tactttgact atcaaagtat catattccta
      781 aatatttagc aatctttttt taatttaaga aaaaatagtt tgacaatcaa aacattttga
      841 ctaataatta agaaatcaag aagttatttt agctattatt ctctattttt ttacgactat
      901 caaattttta tcagaaacaa aggagagtca ttatgtcatt tctagaagga aaaaaaatcg
      961 tcattatggg cgtcgctaac aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga
     1021 aacaaggggc agaaattatc tatacttacc aaaatgaacg aatgaaaaaa tcattagtca
     1081 aattggtcgg cgaagaagca ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg
     1141 aaaaagcaat gaatgccatc ggtgaatatg ctggggagat ccacggcctt gtccatgcag
     1201 ttgcttacgc caacaaagaa gaattgtccg gaaatgtgtc tgatatttcc agagacggct
     1261 atcaattagc tcaagatatc agcagctact ctttaattgc tgtatcaaaa tacgcacaac
     1321 cgtttcttgc accaaacagc gggatcgtta gcatgactta tctaggttca gaacgcgcaa
     1381 tcccgaatta taacatgatg ggtattgcga aagcttctct tgaagctgct atccgttatt
     1441 tggcagctga gttctcgcct aaaggaattc gggtcaatgg aatctcagct ggggcaatca
     1501 aaacattagc tgtcacaggt gttaaagact atcaaaaact gatccagtta tctgaatctc
     1561 gtacgcctga tcagcaaggt gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa
     1621 tcagcccact ttccagcgga atcatcggtg atattatcta cgttgacaaa ggtgtccatc
     1681 tttcataaac ttttgttgca ttctttaaag taaaacatat aaaaatcagg aaaaaacttt
//
LOCUS       DAPBCS010000134         1636 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPBCS010000134 DAPBCS010000000
VERSION     DAPBCS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.146"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(183..1502)
                     /locus_tag="RD521_002912"
     CDS             complement(183..1502)
                     /locus_tag="RD521_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDT7772561.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          301 c          325 g          525 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaat
//
LOCUS       DAPBCS010000135         1589 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPBCS010000135 DAPBCS010000000
VERSION     DAPBCS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.147"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            89..940
                     /locus_tag="RD521_002913"
     CDS             89..940
                     /locus_tag="RD521_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772562.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            961..1244
                     /locus_tag="RD521_002914"
                     /pseudo
     CDS             961..1244
                     /locus_tag="RD521_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          595 a          207 c          320 g          467 t
ORIGIN      
        1 acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta
       61 aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg aacacacctg
      121 gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat tatggccatt
      181 aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt actcgattgg
      241 agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag catcagacat
      301 ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta ctcaattagt
      361 catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc tattcgattt
      421 aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat ctggaagaac
      481 aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg gacacatgta
      541 tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga aacttattaa
      601 acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg gttatgagta
      661 caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc acacgagtat
      721 ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg aaatgaaagc
      781 aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc tacgatatgg
      841 catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat acattttttg
      901 gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa tcgtttgaaa
      961 atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac cttaaagaag
     1021 gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa aacgggtacg
     1081 atgaacattg aataccgaac agaatattta gaattaacga atcaaactga ttttttgttg
     1141 attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa ttgattgatg
     1201 aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct ttatttgtaa
     1261 agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat
     1321 tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag tgaaaggata
     1381 gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc ttttcgctat
     1441 cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga tgagaaaatc
     1501 gtaagagaaa ttgatcgaag atgtgacgaa cggttctgtt gcaaagtttt aaatctacta
     1561 tcaaataagg tagaataata gaaaaagat
//
LOCUS       DAPBCS010000136         1572 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPBCS010000136 DAPBCS010000000
VERSION     DAPBCS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.148"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="RD521_002915"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="RD521_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDT7772563.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            165..899
                     /locus_tag="RD521_002916"
     CDS             165..899
                     /locus_tag="RD521_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HDT7772564.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          588 a          248 c          211 g          525 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacattcatt ataacctcat aggagtgggt tataatgaac aaaaaaaata
      181 taaaagatag tcaaaacttt attacttcaa agcatcatat aaatgaaatt ttgagaaatg
      241 tacatttaaa tacaaatgat aatattattg agattggttc agggaaaggt catttcacgt
      301 ttgaattagc taaaaggtgt aattatgtaa ccgccattga aatagatcct aaattatgta
      361 ggataactaa aaacaaactt attgaatatg agaacttcca ggttatcaat aaagatattt
      421 tacaatttaa gtttcctaaa aataagtcat ataagatata tggaaatata ccctacaata
      481 taagtacaga tataattcga aaaattgttt ttgaaagcac agctacagaa agttatttaa
      541 tagtggaata tggatttgct aaaaggttgc taaatacaaa tcgttcacta gcactatttt
      601 taatgacaga agttgatata tccatattaa gtaaaatccc tagagaatac tttcatccaa
      661 aacctagagt taatagctcg ttaattgtat taaaaagaca cccttcaaaa atatcactca
      721 aagatagaaa acaatatgaa aattttgtta tgaaatgggt taacaaagaa tacataaaac
      781 tattttccaa aaaccaattt tatcaagcct taaaatatgc aagaattgac gatttaaaca
      841 atattagctt tgaacaattc ttgtctcttt tcaatagcta taaattattt aatagataag
      901 ttaaggggtg cataaactgc atcccttaac ttatcttatt ttgcttaaac aatcttggcc
      961 cgtaaactgc ataactcata tgtaaatcgc tcctttttag gtggcacaaa tgtgaggcat
     1021 tttcactatt tccggcaatt acttccatgt aaagtataac ccactatact ttgcaagcaa
     1081 agtgtgggct cgccgagggg tcttgcagac ggctctttcg gctcttttga gccgagtatc
     1141 agcgcaccct tatggtgcaa ttagctgata cctgtttgac gatttgctgc cctacctttt
     1201 cggtaggtat gcaaacctca aaccaaaata aaaaccccct tcgacttcgt cagggggttt
     1261 ttatttatat aaatctccgt aaataatcgc gatttaataa catcttcaat tttatttttt
     1321 tgcgtatttt agaaatgttt tcactaaaaa atatattata caaaaaatac agtaatcgat
     1381 tataattctt tgccagttaa atatcatatc gttattaaaa tatcggaaaa aaatatcaac
     1441 aacaataaat gcaaaagaag accataaaaa ttgtttatta ctcatacata ctctccattt
     1501 ctatcatttt taaatttacc agtcatctct caaaaatctc ctagctattt ttctttttta
     1561 accacatacc gt
//
LOCUS       DAPBCS010000137         1536 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPBCS010000137 DAPBCS010000000
VERSION     DAPBCS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.149"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>1536)
                     /locus_tag="RD521_002917"
     rRNA            complement(<1..>1536)
                     /locus_tag="RD521_002917"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          476 c          337 g          402 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccag
//
LOCUS       DAPBCS010000138         1424 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPBCS010000138 DAPBCS010000000
VERSION     DAPBCS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.151"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="RD521_002918"
     CDS             complement(150..1337)
                     /locus_tag="RD521_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDT7772565.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAPBCS010000139         1406 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPBCS010000139 DAPBCS010000000
VERSION     DAPBCS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.152"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(64..1155)
                     /locus_tag="RD521_002919"
     CDS             complement(64..1155)
                     /locus_tag="RD521_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDT7772566.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          456 a          259 c          206 g          485 t
ORIGIN      
        1 taacacctca gatttgtagt gtctgaggtg tttctataaa cttttataag taaaaaaata
       61 caattatact tcataaaaag acttatacca ctgcgcaaat ttacgaagcc cgtcacgcaa
      121 acttgtactt ggtttaaatt caaaatctcg ttctaacgca ctagtatctg cataagtaac
      181 tggcacatct cctggttgca ttggaactag ttcggtatga gattcaaagt cgtaatcttc
      241 tggtagcacc cctgctgcaa ttagctcttg ttgtagaata tctacaaaat ctagtaggtt
      301 ttccggcgta ttgttaccaa tattataaat atgatatggt gggattggta gcccatcttc
      361 cccatcaatt ttctctggag cgcactgcat aaccctcttt actccttcaa caatatcatc
      421 tatataggta aagtcacgct tgcaattccc ataattgaat atttggatat tatcaccagc
      481 acgaagttta tcagtaaaac caaaataagc catatctgga cgcccagcag gaccatatac
      541 tgtaaagaaa cgtaaccctg tagacggaat attgtaaagt ttagaatatg catgtgccat
      601 tgattcatta cttttcttag ttgctgcata caacgatact tgattgtcta ctttatcctc
      661 tgtagaataa ggaattttct tattagtacc ataaacggaa gacgatgagg catacactaa
      721 atgttccaca ccagattctc cattatcata tgaatgacga caagcttcaa gaatattata
      781 gaatcctatt atgttagatt cgatatacat atctggatta gtaattgaat aacggactcc
      841 tgcctgagct gctaaattca ccacgatact tggtttataa ttatcaaaga tgaaatcaat
      901 tgttgcttta tcagcaatag aacctttgat aaaattccaa gtagatcctg gatggttctt
      961 tgcctccgtt tctatctctg ataatctata gttcttaata gagacatcat agtaatcatt
     1021 catattgtca atgccaatga tttttattga tgactgagta cgtaataact caagaaccaa
     1081 atttgatcca ataaaaccgg ctgcgccagt tattaatatt gttctatttg ataaggacgt
     1141 gtttaattcc agcatttttt agcactcact ttcttttttt cttatgaata catatttgtt
     1201 atatattaaa aaatcttatt tagacttatc tattgtcact tttatcattc taatctttgc
     1261 acgtagtaga ataattatac agataggaaa ttgctaagca acccacttct ttctattatc
     1321 tattaactta ttctaaaacg aatagaaatt attcttgatc acataagtaa ccttaaaaga
     1381 aaaaatttaa tatatgtgtc taattt
//
LOCUS       DAPBCS010000140         1360 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPBCS010000140 DAPBCS010000000
VERSION     DAPBCS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.154"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>1360
                     /locus_tag="RD521_002920"
     CDS             82..>1360
                     /locus_tag="RD521_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HDT7772567.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          461 a          255 c          259 g          385 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcgatgatct atccttacac caatggaaaa
     1261 atagaagcga agaataccca tataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat
//
LOCUS       DAPBCS010000141         1357 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPBCS010000141 DAPBCS010000000
VERSION     DAPBCS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.155"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            27..1328
                     /locus_tag="RD521_002921"
     CDS             27..1328
                     /locus_tag="RD521_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7772568.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          442 a          271 c          252 g          392 t
ORIGIN      
        1 acaacacaat caaaggagcg ttctgtatgc ctgatcttca tcatatcaaa ctactacttg
       61 gaatcaaaga taaaaacatt ttcatcacaa atgtggaatc gaagagcatt aagaatatca
      121 agagtcttgt tgtttctgcg acgttaagta aagaaattcg tcgctgtcct ttgtgtaaac
      181 agatgaatca tgaaggcatg atcgttaaga acggaaagaa aaaatcgctc attcaactta
      241 ataaatgtgc gaaccaactg acttatcttg ctttagcgaa acaacgctac cactgtcgtg
      301 gctgccacac ctattttacg gcgaatacct acattgtcga tagaaactgc tttatcgcta
      361 aacaagtgcg ctataaaatt ttagaagagc taactgaaaa acaagcaatg acgacaattg
      421 ctaagcattg cggcgtttct tggtcgaccg tttctcggac actcgcttct ctgatcccca
      481 tgacaaaagt aaaaagaaat tggttaccgc gttgtttatt aatggatgaa tttcgttcac
      541 tgaaaaatca agtcggtccc tactcctttt cttgtatgga tggcgatact ggaaaattac
      601 tggatattct tccttcacgg aagaaaaagg ctctcgtttc ttattttatg caatttgaac
      661 gacgcgcacg attgaaggta aaggtcatcg tgacggacat gaatgtttct tatgcctctc
      721 tgataaagga atgttttcca aaggcaaaac tcgttgtgga tcgttttcac attgtcaaac
      781 atctcattcg taaatttgag gatattcgcg tacgaatcat gaaaagcttt gatcgaaacg
      841 atccaacaca agccaaatat tatcgacaat taaaggcgtt gtctcggctt ctgatcaaac
      901 gacaggacaa gcttgtttat gacaaatgga ccaaatggcg caattttggt tgggcgtatc
      961 tcaccgaaag tgaagtggtg gatcgtctgc tttcgatctc tgacgaatta cgaatcgcct
     1021 atttttacta tcaagaaatt cttcaagcgt ttcacgacaa agaggcggac actttcttta
     1081 aacttgtaag aacgatgccc aattccgttc caaaagaact gcattctata aaaaaagctt
     1141 ttataaacta tgaatccggc attcgtttag ccctcgaatt gccttattca aatgccaaaa
     1201 tagaaaatct gcatacccac atcaaagcat tgaaacgagt cgcttatggc tttcgaagct
     1261 ttcggaaaat gaagaccaga attttcttac taaataatct gattacctat gaatcaaaaa
     1321 acatctaaag tagaaaagga attctacctt agatgtt
//
LOCUS       DAPBCS010000142         1334 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPBCS010000142 DAPBCS010000000
VERSION     DAPBCS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.157"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(110..1258)
                     /locus_tag="RD521_002922"
     CDS             complement(110..1258)
                     /locus_tag="RD521_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7772569.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          385 a          235 c          209 g          505 t
ORIGIN      
        1 ggagcttctt gggaatagag catacttgat agcgtatttc tttactaaca gcggtgtctc
       61 ttatttacca agctatcccc gtagttccta cggttcttga tgattggatc taagccaatc
      121 ccatagaatg tagtcttaga ccttcggtca gaatattgat actagcattg atatcacgat
      181 catgatgagt atgacaaaca ggacaagtcc attctcgaat ttcaagagat ttcttgccat
      241 ctttatgtcc gcactctgaa caaatctgac tagacggaaa ccatttatct actttgatga
      301 tttttcgtcc ataccagtca gccttgtatt gtaatttagt cacaaacttt gaccaagaaa
      361 catcagaaat acttttagct aatttatgat ttcgcaacat accttttgtg tttaagtctt
      421 caatacagat aatatcgtga tttttgatca tttctgtact caacttattc agaaaatcag
      481 tacgttgatt cattactttt tcatgcaatc tagctacttt gcgtttttgt ttttgataat
      541 ttttagcttc aaatagattg gtacctttat ttttagccaa taaagcacgc ctagacaatt
      601 tgcgttgttc acgctttagt ttcttttcca ttttggacgt gaatttatta ttatcaattt
      661 tttgtccatc agaaagaatc gcaaagtcca taatacctaa gtcaatacca attgtggaat
      721 tagttttagg taattcgata atttcttctt tacacaacaa agaaatatag tatttaccgc
      781 tagaatgacg tgatattgta gcagatttga taatcccttt tggttgtcta tgaagcttaa
      841 ttcttactaa tgacttcaat ttaggaactt tgatgaattt actatcaatc aaagcaattg
      901 taccattttg attattcgtt gtataactct gaacaggatt tttcttactt ttgaaacgtg
      961 gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc tttatctaaa ttaagttggg
     1021 cattggctaa agcaaggcta tcaatttctt tcaaaaacgg aaattctttc ttgtatttag
     1081 ctggtgtcgg aaaagtcatt tttttagaag aatcattttt aacttcttca tacgcctttt
     1141 ttcggtcatc aagcattaga ttgtagacct tacggacaca accaaaagat ttggcaaaga
     1201 agatttcttg ttcctctgtt ggatagattc tgaatttgta tgcttttagt tgttccataa
     1261 agttcaccta ctttctattt atgattgttt ccttgatttt ggatatactt cttaatcaca
     1321 tcaattggtg cgcc
//
LOCUS       DAPBCS010000143         1333 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPBCS010000143 DAPBCS010000000
VERSION     DAPBCS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.158"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..397
                     /locus_tag="RD521_002923"
     CDS             5..397
                     /locus_tag="RD521_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7772570.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            398..>1333
                     /gene="aac(6')-Ie"
                     /locus_tag="RD521_002924"
     CDS             398..>1333
                     /gene="aac(6')-Ie"
                     /locus_tag="RD521_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDT7772571.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL"
BASE COUNT          547 a          111 c          221 g          454 t
ORIGIN      
        1 cctattgaaa ctattgttta ttgatgataa tatggttttt gatattgata atttaaaagg
       61 atttcttaat gataccagtt catttgggtt tatagctaaa gaaaataata aaattatagg
      121 atttgcatat tgctatacac ttttaagacc tgatggaaaa acaatgtttt atttacactc
      181 aataggaatg ttacctaact atcaagacaa aggttatggt tcaaaattat tatcttttat
      241 taaggaatat tctaaagaga ttggttgttc tgaaatgttt ttaataactg ataaaggtaa
      301 tcctagagct tgccatgtat atgaaaaatt aggtggtaaa aatgattata aagatgaaat
      361 agtatatgta tatgattatg aaaaaggtga taaataaatg aatatagttg aaaatgaaat
      421 atgtataaga actttaatag atgatgattt tcctttgatg ttaaaatggt taactgatga
      481 aagagtatta gaattttatg atggtagaga taaaaaatat acattagaat cattaaaaaa
      541 acattataca gagccttggg aagatgaagt ttttagagta attattgaat ataacaatgt
      601 tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg attatcatta
      661 tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc caaattattg
      721 gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga aaaaagaaag
      781 aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa taagggcata
      841 ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac acgagggcaa
      901 aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa atgttaaggc
      961 aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta ttgaaataat
     1021 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
     1081 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
     1141 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
     1201 attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc
     1261 tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca
     1321 aatgcacggt tta
//
LOCUS       DAPBCS010000144         1312 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPBCS010000144 DAPBCS010000000
VERSION     DAPBCS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.159"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(156..1297)
                     /locus_tag="RD521_002925"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="RD521_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDT7772572.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DAPBCS010000145         1311 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPBCS010000145 DAPBCS010000000
VERSION     DAPBCS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.160"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="RD521_002926"
     CDS             200..742
                     /locus_tag="RD521_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772573.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1248
                     /locus_tag="RD521_002927"
                     /pseudo
     CDS             898..1248
                     /locus_tag="RD521_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          255 g          376 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DAPBCS010000146         1302 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPBCS010000146 DAPBCS010000000
VERSION     DAPBCS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.161"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            52..>1302
                     /locus_tag="RD521_002928"
     CDS             52..>1302
                     /locus_tag="RD521_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDT7772574.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAPBCS010000147         1215 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPBCS010000147 DAPBCS010000000
VERSION     DAPBCS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.162"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..>1215
                     /locus_tag="RD521_002929"
     rRNA            2..>1215
                     /locus_tag="RD521_002929"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          368 a          242 c          352 g          253 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctaggatgtt ggcttagaag cagccaccat ttaaagagtg cgtaatagct cactagtcga
     1141 gtgaccctgc gccgaaaatg taccggggct aaacatatta ccgaagctgt ggagtacacc
     1201 tttaggtgta ttggt
//
LOCUS       DAPBCS010000148         1191 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPBCS010000148 DAPBCS010000000
VERSION     DAPBCS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.163"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>1191)
                     /locus_tag="RD521_002930"
     rRNA            complement(<1..>1191)
                     /locus_tag="RD521_002930"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          249 a          358 c          282 g          302 t
ORIGIN      
        1 cgggtgttac aaactctcgt ggtgtgacgg gcggtgtgta caaggcccgg gaacgtattc
       61 accgcggcgt gctgatccgc gattactagc gattccggct tcatgcaggc gagttgcagc
      121 ctgcaatccg aactgagaga agctttaaga gattagctta gcctcgcgac ttcgcaactc
      181 gttgtacttc ccattgtagc acgtgtgtag cccaggtcat aaggggcatg atgatttgac
      241 gtcatcccca ccttcctccg gtttgtcacc ggcagtcttg ctagagtgcc caactgaatg
      301 atggcaacta acaataaggg ttgcgctcgt tgcgggactt aacccaacat ctcacgacac
      361 gagctgacga caaccatgca ccacctgtca ctttgccccc gaaggggaag ctctatctct
      421 agagtggtca aaggatgtca agacctggta aggttcttcg cgttgcttcg aattaaacca
      481 catgctccac cgcttgtgcg ggcccccgtc aattcctttg agtttcaacc ttgcggtcgt
      541 actccccagg cggagtgctt aatgcgttag ctgcagcact gaagggcgga aaccctccaa
      601 cacttagcac tcatcgttta cggcgtggac taccagggta tctaatcctg tttgctcccc
      661 acgctttcga gcctcagcgt cagttacaga ccagagagcc gccttcgcca ctggtgttcc
      721 tccatatatc tacgcatttc accgctacac atggaattcc actctcctct tctgcactca
      781 agtctcccag tttccaatga ccctccccgg ttgagccggg ggctttcaca tcagacttaa
      841 gaaaccgcct gcgctcgctt tacgcccaat aaatccggac aacgcttgcc acctacgtat
      901 taccgcggct gctggcacgt agttagccgt ggctttctgg ttagataccg tcaagggatg
      961 aacagttact ctcatccttg ttcttctcta acaacagagt tttacgatcc gaaaaccttc
     1021 ttcactcacg cggcgttgct cggtcagact ttcgtccatt gccgaagatt ccctactgct
     1081 gcctcccgta ggagtttggg ccgtgtctca gtcccaatgt ggccgatcac cctctcaggt
     1141 cggctatgca tcgtggcctt ggtgagccgt tacctcacca actagctaat g
//
LOCUS       DAPBCS010000149         1157 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPBCS010000149 DAPBCS010000000
VERSION     DAPBCS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.164"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            115..1134
                     /locus_tag="RD521_002931"
     CDS             115..1134
                     /locus_tag="RD521_002931"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDT7772575.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          406 a          183 c          260 g          308 t
ORIGIN      
        1 agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca atggaaatta gtcccaactt
       61 taagaaatgt atactataat ggaaattatc aattatttat ttggaggaat atccatgaaa
      121 caaaaaatcg cagttcttgg tcctggatct tggggcactg ctttagcaca aacattggca
      181 gaaaacgggc atgatgtccg tatttgggga aatgtacctg aacagattga tgaaatcaat
      241 aagtatcata caaatcagca ttttttacct gatttgacga tcccagaatc gattatcgga
      301 tataaagaat tggcagaagc agtggataac gcagatgcta ttttatttgt tgttccaaca
      361 aaagcgattc gttcagtcgc acaggaactt attggtaaaa tgaatactaa gccaatcatt
      421 atacatgcaa gtaaaggact agaacaagat acgcataaac gaatctcaga agttttggct
      481 gaagaaattc cagaagaaaa aagacaggca attgttgtat tatctggtcc tagccatgct
      541 gaggaagtgg cagtacatga tatcacaaca attacagctg caagtattga tcaaaaagca
      601 gcagcctatg tacaaaaact gttcatgaat gattatttca ggatttatac aaataatgat
      661 gtgattggcg tagaaactgg tgcagcactg aaaaatatca ttgcaattgg tgccggcgct
      721 atccatggat taggattcgg agatgatgca aaagcagcaa tcatgactcg aggattggca
      781 gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc tgacatttat cggtttgagt
      841 ggtgtggggg acttgatcgt tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt
      901 aacttgttag gacagggaca taagctggaa gaagttctag aaaacatggg aatggtcgtt
      961 gagggtgtgg caacgacaaa agcagcagta gagctagctc aacagctagg tgttgagatg
     1021 ccaattactc aaacaatcta taatgttttg tataatggag aagatatcaa aaaagcagca
     1081 aaagaaatca tgttacgtga cggaaaaatg gagaatgaat tttctcttcg ttaaatagat
     1141 gatcaatagt taatcgt
//
LOCUS       DAPBCS010000150         1153 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPBCS010000150 DAPBCS010000000
VERSION     DAPBCS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.165"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            60..260
                     /locus_tag="RD521_002932"
     CDS             60..260
                     /locus_tag="RD521_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772576.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            451..>1153
                     /locus_tag="RD521_002933"
     CDS             451..>1153
                     /locus_tag="RD521_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772577.1"
                     /translation="MTKKIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     KNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKL"
BASE COUNT          478 a          109 c          220 g          346 t
ORIGIN      
        1 acttgtatac acgtattcaa ataaaaaagc agttattaca tgaaggatta agtggtaata
       61 tgttaaaaag aattgcatgg gataaagata ataaaacaga tattgagctt aaagaatata
      121 aatacaatta tttctcgaga tgtatagata gaaagttact gtttaaaaaa gaaggattag
      181 aatttagttt tgatgaagat gaatttgaaa aattagtgat ggactggcgt aagaatttac
      241 tggatacggt acaattctaa aagcaattga ggtcgaaatt ccgatacttg aatcatgatc
      301 tatattatcg tgttatgagt gaaatttcta ataaagataa gatatttcat ttggacagtc
      361 atgtgaatat gagcttaaca attaaacgta tttatgatct tttatatgtt tataaacatt
      421 aatgaggtgc taaaattgac aacaataact atgacaaaaa aaatagaagt atatgttgat
      481 ggatcacaag aagaaaggga ttcctttttt gatttttttt ggaaaaatag tagagaatat
      541 gtaaaagcgt tgaatctggt atataaacag ctaatgtttg aagagttgat aaaagatgca
      601 attaaagaaa tggatcaagg ttatgctgag agagaagaaa gtattcaaaa tcggattgaa
      661 aagttagcat atactgatcc aatagatcaa aaaaaacttg ataaagcaaa agctgaatta
      721 tctaaattga gaaatgaaaa aaataaagaa gcgagagaaa tattaacgca agcgataggg
      781 ttgaagagtc aaacgagaac acgagatgta gcaaaaaaat tagactttga ctttggagat
      841 tttgttgatt ccgcaaatat gaaagctagt aaagattttg gcaatgattt tatcggaatt
      901 gtgaccggac aaagaacgcc tagaatatat aaaaatagaa acaaatatgg attactacct
      961 tttagaggtc gagatattaa aatccataaa gatgataagt tttatattta tttacctaaa
     1021 ggctttaggt ttaaagtgaa tttaggtagc aagccaagaa aagcaagaga tgttgcttca
     1081 gttttacaac acattatgga tggcgattac aaattaaatc aaagtaatct tatttttgat
     1141 ggaagaaaat tat
//
LOCUS       DAPBCS010000151         1137 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPBCS010000151 DAPBCS010000000
VERSION     DAPBCS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.166"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..355
                     /locus_tag="RD521_002934"
     CDS             <1..355
                     /locus_tag="RD521_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772578.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="RD521_002935"
                     /pseudo
     CDS             615..910
                     /locus_tag="RD521_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="RD521_002936"
     CDS             985..>1137
                     /locus_tag="RD521_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDT7772579.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAPBCS010000152         1119 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPBCS010000152 DAPBCS010000000
VERSION     DAPBCS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.167"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            143..400
                     /locus_tag="RD521_002937"
     CDS             143..400
                     /locus_tag="RD521_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772580.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="RD521_002938"
     CDS             complement(632..1063)
                     /locus_tag="RD521_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDT7772581.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          425 a          143 c          144 g          407 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt gttactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaa
//
LOCUS       DAPBCS010000153         1062 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPBCS010000153 DAPBCS010000000
VERSION     DAPBCS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.168"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(37..993)
                     /locus_tag="RD521_002939"
                     /pseudo
     CDS             complement(37..993)
                     /locus_tag="RD521_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          177 c          159 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcattcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DAPBCS010000154         1054 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPBCS010000154 DAPBCS010000000
VERSION     DAPBCS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.169"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            67..885
                     /locus_tag="RD521_002940"
                     /pseudo
     CDS             67..885
                     /locus_tag="RD521_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          382 a          152 c          212 g          308 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgattt atccaagtta ccaagaatcc aaaaaaagat tcaagcaagc atatggtaag
      121 ttaaaagagg tttcatccat ggaatccatt gatgaacata cgccacttga aatccgtatc
      181 gagtacgtca aagcatttca agaattgaat cgtgcgtatg aatcgttagt gacttatgat
      241 gattacaatg atgacatggt gcaatcttca actttgaaca atcaaattca acttttggaa
      301 gaacaaattg gcgtgtacga gaccgtgaag ggttcattga tggaggaaga accagaaaaa
      361 gagggagaag atttttcaac gatcgaattt tatagtgaaa ataccactaa gttgtatgat
      421 attgattcta cttatattga tcaattacta ggaacttatg ctgcgaatag ttcagatgca
      481 cgagaagaaa ttgaaaaagc tttggctaaa ttgaataaaa cagaaggagt caaagaagtt
      541 taccgacaaa ttttaaatgc atttgatgtg gggactttag atagtaatga agatatcttt
      601 gtcattaaac gtcgctattt tacacaagca agtgataatt tgattcatgc cttttccaat
      661 gagtggtttg tttctgagaa agaattacat gcttctaatc tgcaatatat gccaggtgaa
      721 gatccaattc ctaatatgaa agcaattatc aacagtaagg attacgaagg atataaagcc
      781 aaacatccag aagcgaaacc atttaaatac ccacaagaaa tgaagcgagc atggcgtaaa
      841 atgttagatg atgagttgat tccacttgaa aatgaattga ggtaaacgag aagagttaag
      901 aaaattgtac gattcaaaga tagtagtaaa gtggaagtgt agaaaatagg gatagatgtc
      961 tttttacttg ttcacatttt ataacggagt atttaagaaa gctattgaat aatgattctt
     1021 ccatcttttt cattcattta ccggttctgt tgca
//
LOCUS       DAPBCS010000155         1018 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPBCS010000155 DAPBCS010000000
VERSION     DAPBCS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.171"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            155..>1018
                     /locus_tag="RD521_002941"
     CDS             155..>1018
                     /locus_tag="RD521_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HDT7772582.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKLNL"
BASE COUNT          392 a          121 c          207 g          298 t
ORIGIN      
        1 tattttagtt gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa
       61 aacccttaaa attggttgca cagaaaaacc ccatctgtta aagttataag tgaccaaaca
      121 aataactaaa tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc
      181 agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag
      241 agaaaagaaa atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa
      301 ggctgaaaga gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg
      361 cgaaagaaag ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg
      421 gaggagagca atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca
      481 aaagccaaca gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga
      541 agaattaaat aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa
      601 aaaaattaat aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa
      661 agataattct tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa
      721 gaatacagaa aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa
      781 attagactat gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc
      841 ggatatacaa tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat
      901 gaccgatgat gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg
      961 taaaaggtta atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaacctt
//
LOCUS       DAPBCS010000156          983 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPBCS010000156 DAPBCS010000000
VERSION     DAPBCS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.172"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..461)
                     /locus_tag="RD521_002942"
     CDS             complement(<1..461)
                     /locus_tag="RD521_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDT7772583.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(747..968)
                     /locus_tag="RD521_002943"
     CDS             complement(747..968)
                     /locus_tag="RD521_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDT7772584.1"
                     /translation="MKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRV
                     AYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          315 a          187 c          131 g          350 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaag
      541 atatatatgt actaccttat aattaataag taaaaggtag tacataaata aggaaaagct
      601 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
      661 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      721 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
      781 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
      841 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
      901 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
      961 tctttcaatg gcttctcttt tag
//
LOCUS       DAPBCS010000157          951 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPBCS010000157 DAPBCS010000000
VERSION     DAPBCS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.173"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..>951
                     /gene="bsh"
                     /locus_tag="RD521_002944"
     CDS             6..>951
                     /gene="bsh"
                     /locus_tag="RD521_002944"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDT7772585.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPII"
BASE COUNT          330 a          127 c          170 g          324 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata g
//
LOCUS       DAPBCS010000158          924 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPBCS010000158 DAPBCS010000000
VERSION     DAPBCS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.174"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            499..897
                     /gene="spxA"
                     /locus_tag="RD521_002945"
     CDS             499..897
                     /gene="spxA"
                     /locus_tag="RD521_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDT7772586.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          351 a          123 c          151 g          299 t
ORIGIN      
        1 ttttaataca gtctcaatat aactattcaa acataaaata acgttatttg atttatttgt
       61 ttatagtaaa ttttatttag taatcattgc tttaaaatac tacgcttata taacagagaa
      121 aataagcgtt ataataaaga acagtattat ttaaaatgac agaaaacatt actattaaag
      181 aaataaaaaa cataaaaaat cttaagaaat cggtttcatt tagcttttga gataaaataa
      241 tagtacagtt ttatacgctg tttcatatgt ttagaaaatt tgaattagat ttgaaaactc
      301 taatttttaa atgataaaaa aagcgagaat gctggtttta ctgtatatta accttagata
      361 actttcattt ttttaaaaaa attatgaaaa aaaggtagct atttaaaagt tgattatgta
      421 taatagaaac agatgggaga ttattatgat cttctgttac aaaaaagtga ttcttatcac
      481 gaaaaggagt ggtgcacgtt gttaacatta tatacttccc caagttgtac ttcctgcaga
      541 aaggctcgtg cgtggttgca agaacatcag attccatttg ttgaacgtaa tattttttca
      601 gaaccattga acagttctga attaaaagcg attttacaaa tgacagaaga cggaacagaa
      661 gaaatcatct cgacacgttc caaagtattc caaaaactga acatggattt agatgatctg
      721 ccgttacaag aattattgga acttgtgcaa aacaatcctg gattgcttcg ccgtccaatc
      781 atgattgatg ataaacgtct gcaagttggt tttaatgaag atgaaattcg tcggttccta
      841 ccgcgcgatg tacgacaatt agaattacgt caagcacaat tgatggctgg attatagaag
      901 gataatacga aacttggaaa agac
//
LOCUS       DAPBCS010000159          858 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPBCS010000159 DAPBCS010000000
VERSION     DAPBCS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.175"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..751
                     /locus_tag="RD521_002946"
     CDS             <1..751
                     /locus_tag="RD521_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDT7772587.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDIN
                     RSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTL
                     GYETPVGYRNQRLAQRILDNELGCANASEAV"
BASE COUNT          286 a          159 c          176 g          237 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataaagatg ccgttctagt
      361 aaaaaaagca tttagccgta tcaaacaacc tctgacagag gttgagattt ttcatactga
      421 tcgtggaaaa gagtttgata accaagctat tgatgaatta ttaacaactt ttgacatcaa
      481 tcgatcattg agtcataaag gctgtccttt tgataatgcc gtagctgaat caacttataa
      541 gtcgttgaaa gtagaatttg tctatcaata cacatttgaa accttacaac aattggattt
      601 ggagttattt gactatgtca attggtggaa ccaccttcgg ttgcacggta cacttggcta
      661 cgagacaccg gttggttacc gtaaccagag attggcgcag cgaatccttg ataatgagct
      721 cggatgtgct aacgctagcg aggcagtcta actttaactg ttagctcctg ccgaagatcg
      781 tcacatccga ggaggctcat tgtcaaggac aatcggaata gcatacggaa agaagttctg
      841 caccttataa aatttgtc
//
LOCUS       DAPBCS010000160          850 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPBCS010000160 DAPBCS010000000
VERSION     DAPBCS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.176"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            27..>850
                     /locus_tag="RD521_002947"
     CDS             27..>850
                     /locus_tag="RD521_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7772588.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDF"
BASE COUNT          292 a          149 c          164 g          245 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taatgataga gtcttatttc aaaataaatc aatctgttgc gaaaactgcg catttcttga
      121 atcgttcaag acaaacgatc cataaagtat acctgttctt caagcaagga aaatcagcct
      181 tagagtacta tcaacagtat aagaaaaaca aatcaaactg tggtagacgc ccgcttgttt
      241 tacctgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctattc gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttaat ccttctgact taccgatgaa aggcaagcgt aaaccaaatg
      421 gacatcaaga aagacgtgga aaacaagctt tccgtcgctc tattcatgaa cgtgaaaaag
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtttga
      541 aacataaaag tgctgtaatt accttagttg agcgattatc aaaagttatc atcacattga
      601 aaccgtgtgg tagacaagcg attgatattg aaaacaaatt aaatcattgg tttgaatctg
      661 taccgaaaaa tctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      721 aacagatcag taatgccaat gatattgcca tttatttcgc tgatccagga acgccgtctc
      781 aaagaggcct aaacgagaat tctaacggat tgttacgtag agatggttta ttgaaatcta
      841 tggatttcaa
//
LOCUS       DAPBCS010000161          847 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPBCS010000161 DAPBCS010000000
VERSION     DAPBCS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.177"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            69..644
                     /locus_tag="RD521_002948"
     CDS             69..644
                     /locus_tag="RD521_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDT7772589.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(713..>847)
                     /locus_tag="RD521_002949"
     CDS             complement(713..>847)
                     /locus_tag="RD521_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDT7772590.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          236 a          144 c          181 g          286 t
ORIGIN      
        1 cgttctttta aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg
       61 ctaacaaaat gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta
      121 ttgggatcaa ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg
      181 attctatcgg aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag
      241 gattgtccag cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc
      301 caatccaaat tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag
      361 ataaaaacag cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg
      421 tttcttcgat cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga
      481 tcgttcagct attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg
      541 cgggtacaga ctaccttgat cgcttgttgt ctatcgtcgt tgtttcatta gtcatcacta
      601 tgttgcccca acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat
      661 tagcaaaata atgctaatct ggctctgttt tttcatttta ttatttgtca aattaagcga
      721 gacaaaactt tccatttacg taactcaaaa aaacttctaa tggtgtttgg taatttagtg
      781 attttctagg gatatggttt cttttggacg caacggatga gatgaatcct tgattgactt
      841 ggttgaa
//
LOCUS       DAPBCS010000162          840 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPBCS010000162 DAPBCS010000000
VERSION     DAPBCS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.178"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            71..>840
                     /locus_tag="RD521_002950"
     CDS             71..>840
                     /locus_tag="RD521_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDT7772591.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRN"
BASE COUNT          305 a          157 c          143 g          235 t
ORIGIN      
        1 tacaaaaaag gaagtccctt ctgtagaata aagttaacga caaccaattc acagaaaaga
       61 ggacttccct atgaatgatt ttactacaga aattgtgcaa actctagtca ctaaaggcga
      121 tttaaatgaa ttattccgtt cgcacttaga aaaagcgata aacacactcc tacggactga
      181 attaacggct tttttagatt acgaaaaata tgatcgcact ggttttaatt caggtaattc
      241 gagaaacggt tcttactttc gatcaatcaa aaccgaatat ggtgaattaa cattggaaat
      301 acctagagat cgtaatggtg agtttaaaca acaaacttta ccagcctaca aaagaacaaa
      361 cgatacattg gaaaccacta ttatccattt attcgaaaaa ggtgttacga tgtctgaaat
      421 tgctgatttg atcgaaaaaa tgtacggtca tcactatact ccacaaacca tgtccaacat
      481 gactaaagtt ctgactgaag aagtaaatgc ctttaaatcc agagccttaa atgataagta
      541 tgtcgctatt tttatggacg ctacttacat tccactaaaa cgtcaaaccg tatccaaaga
      601 agcgatttat attgccattg gtatacgaga agacggcact aaagaagtac tgagttatgc
      661 gattgctcca actgaatcaa catacgtttg gaatgagctg ctacaggata ttaactccag
      721 aggagttcaa gaagtcttgc tttttattac ggacggctta aaaggcatga aagatactat
      781 ccatcaaatt tatcctaaag caaaatatca gcattgttgt atccatgtat ctcgtaacat
//
LOCUS       DAPBCS010000163          839 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPBCS010000163 DAPBCS010000000
VERSION     DAPBCS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.179"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>839
                     /locus_tag="RD521_002951"
     CDS             82..>839
                     /locus_tag="RD521_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDT7772592.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVK"
BASE COUNT          278 a          156 c          163 g          242 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaaca
//
LOCUS       DAPBCS010000164          780 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPBCS010000164 DAPBCS010000000
VERSION     DAPBCS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.180"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>780
                     /locus_tag="RD521_002952"
     CDS             <1..>780
                     /locus_tag="RD521_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDT7772593.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKH"
BASE COUNT          277 a          152 c          140 g          211 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
//
LOCUS       DAPBCS010000165          775 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPBCS010000165 DAPBCS010000000
VERSION     DAPBCS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.181"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            381..>775
                     /locus_tag="RD521_002953"
     CDS             381..>775
                     /locus_tag="RD521_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7772594.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTP"
BASE COUNT          275 a          119 c          165 g          216 t
ORIGIN      
        1 aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat
       61 tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc
      121 cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc
      181 aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt
      241 tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa gtatagtgtg
      301 ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc
      361 taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag
      421 ctggactaaa aggcatgcaa tttcataatc aaagagagcg aaaaagtaga acgaatgatg
      481 atattgacca tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa aatattgatt
      541 acaacgaacg tgtcaaagaa attattgaat cacaaaaaac aggtacaaga aaaacgagga
      601 aagatgctgt tcttgtaaat gagttgctag taacatctga ccgagatttt tttgagcaac
      661 tggatccagg agaacaaaaa cgattttttg aggaaagtta taaattattt tccgaacgat
      721 atggcaagca aaatattgct tatgcaacag ttcataatga tgagcaaacc cctca
//
LOCUS       DAPBCS010000166          749 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPBCS010000166 DAPBCS010000000
VERSION     DAPBCS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.182"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..>749
                     /locus_tag="RD521_002954"
     CDS             1..>749
                     /locus_tag="RD521_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HDT7772595.1"
                     /translation="MLKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIG
                     FNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHFFR
                     KGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAIFMDATYI
                     PLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDVLLF
                     ITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          233 a          160 c          154 g          202 t
ORIGIN      
        1 attctaaaga ctctagcgaa caaaggcgat ttgaatgaat tattccgtgt ccatttggaa
       61 aaagctgtca atacgcttct caaaacggag ttaacggctt tcctcgatta cgaaaagtac
      121 gatcgcattg gttttaacac gggtaattct cgtaacggct cctatgaccg tacggtcaag
      181 accgagtacg gggaacttca tctccagatt ccgcgcgacc gcaacggcga gttcaagcaa
      241 cagactgttc ctgcttatag acggacgaat gataccttag aggagaccgt cattcacttc
      301 ttccgaaaag gtattaccat gtcggaaatc gcagacttga ttgagaaaat gtatgggcat
      361 cactacacgc cccaaaccat gtccaacatg actaaagtgc tgactgaaga agtaaatgca
      421 tttaaagtta gaactctaaa tgataagtat gtcgctattt tcatggatgc tacttatatt
      481 ccattaaaac ggcaaaccgt ctccaaagaa gcaatctata ttgccattgg tatacgagaa
      541 gacggcacta aagaagtact aagttatgcg attgctccga ctgaatcgac ctacgtttgg
      601 aatgagttgt tacaggacat ttactccaga ggggttcatg atgtcttact gtttattacc
      661 gatggcttaa aaggcatgaa agatacgatt caccagattt atcctaaagc taaataccaa
      721 cattgctgtg ttcacgtttc ccgtaatat
//
LOCUS       DAPBCS010000167          705 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPBCS010000167 DAPBCS010000000
VERSION     DAPBCS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.183"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(44..>705)
                     /locus_tag="RD521_002955"
     CDS             complement(44..>705)
                     /locus_tag="RD521_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HDT7772596.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          195 a          167 c          130 g          213 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggtt tggttatcaa
      361 attcctttcc ccggtctgta tgaaaaagct tgacgtctgt taaagaatac ggtatccgtc
      421 caaaggcttc ttttaccaat gaggcatctt tcttctcacc acaggaataa ccaataattt
      481 ctcgattaaa caaatcaagt attaagcaga tataatgcca ctttttcccc acgcgaacat
      541 aagtaagatc cgtaacgatg gcttccaatg gctgttcttg tgtaaaggtc cgatctaata
      601 cattcgctgt tttcgcttca ttacaagctg ttcgttgccc tttaaaatga gcgatcgtat
      661 aggtagatgt caatccgcgg cgtttcatga ttcgaccgat tcggc
//
LOCUS       DAPBCS010000168          701 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPBCS010000168 DAPBCS010000000
VERSION     DAPBCS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.184"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..592
                     /locus_tag="RD521_002956"
     CDS             <1..592
                     /locus_tag="RD521_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDT7772597.1"
                     /translation="KEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGG
                     ALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQ
                     EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNG
                     YVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          196 a          138 c          179 g          188 t
ORIGIN      
        1 aaaagaattt ccgatcccga tttcttggac aacagatgtc aatgcggctg cctatggtga
       61 gtatgtcttc ggcagtggca aaggattgag cagtgtcgtt tactatacga tcggtacggg
      121 tgttggcggc ggtgccttac aagaagggcg ctttatcgaa gggtttagtc atccagaaat
      181 ggggcatatg ctcgtcgtcc ctcatccaaa agatgacttt gctggcagct gtccatttca
      241 tggcaactgt ttagaaggga tggccgctgg acccgccatc gaaaaacgct tagggaaaaa
      301 aggacaagag gttgcagaag atgatcctta ttgggaaatc gaagcctcct atattgctca
      361 atgtgcctac aatacaacct tgatgttttc tccagatgtg atcatttttg gcggtggtgt
      421 gatgaaacaa cgccatatgc tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg
      481 gtatgtcgaa acacctgagt taagcaaata tatcgtgacc ccagcattag aagacaacgc
      541 tggaacgctc ggttgtttgg cattggctcg agaagcggtc ttacattcat aaacgaaaaa
      601 aggctcttcg acatttagtg ttggtagaga aagatacaac tgaaaattta gtcataacca
      661 gttaggcaga gtgcttaact ggtttttgtt ttacttggaa g
//
LOCUS       DAPBCS010000169          670 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPBCS010000169 DAPBCS010000000
VERSION     DAPBCS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.185"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            154..417
                     /locus_tag="RD521_002957"
     CDS             154..417
                     /locus_tag="RD521_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDT7772598.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="RD521_002958"
     CDS             417..>670
                     /locus_tag="RD521_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDT7772599.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAPBCS010000170          640 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPBCS010000170 DAPBCS010000000
VERSION     DAPBCS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.186"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..283
                     /locus_tag="RD521_002959"
     CDS             <1..283
                     /locus_tag="RD521_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDT7772600.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DAPBCS010000171          633 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPBCS010000171 DAPBCS010000000
VERSION     DAPBCS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.187"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..390)
                     /locus_tag="RD521_002960"
     CDS             complement(<1..390)
                     /locus_tag="RD521_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDT7772601.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTS"
BASE COUNT          210 a          114 c          127 g          182 t
ORIGIN      
        1 ggaagttgtg aatcgttgaa tacgcaagaa atcagcgatt cttaatgcat cgtttcgatc
       61 agttttattc tcatcaaaca tgcgactaaa ttgtttcacc cgaaaaggat tttcaatcgt
      121 tacaagtgtg ttgagcttct ttagtttctc atcttcatga aaaaacatgg aaggatggaa
      181 gctgtacatg gacgttgact ccatgccaat cacaatttga tcaaagtggt agctgtcatt
      241 gaattcaaga attgtttctc gtatgaatga agcaccttcg atgtcattgg caacagagat
      301 ttcagataag atagacaatt gattgtcgtc tgttaaaaaa caaacatcta gtttttcaga
      361 gctaacgtca ataccgacaa ataatttcat ggaagaagga cctcctttcg atataaattt
      421 tggaaaatgc ttcgatactg tgggatttcc cagaaactca ctgtatagcc acaacctcgc
      481 ctaatagaat acggtgataa aagagctaga agctgccttc caaactacta tttgaaagtc
      541 tagtccatta tcgaaacagc taatgagttg gtagtgataa cagtggttcg ggtaacagac
      601 taaacagggt agacaaacgc tacaggagga aat
//
LOCUS       DAPBCS010000172          556 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPBCS010000172 DAPBCS010000000
VERSION     DAPBCS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.188"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..346)
                     /locus_tag="RD521_002961"
     CDS             complement(152..346)
                     /locus_tag="RD521_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772602.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYPFV"
BASE COUNT          171 a          113 c           88 g          184 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaaacaaa tggataatct tctgtcgcat
      181 cgacgaacgt cttcgtagtc agatttaacg ttaaaatatc gctactttga taagcttttg
      241 ccgctttgtc tgtgacgaca aaagatctgc ctgtttgaat ttctgtcaca aaaacgagat
      301 tgtctctgct tttttcaaca acaactccaa tcgttgaatg aaccatttct gcttcctccc
      361 ttctctttct tttgatataa tgtagtcgca aatcgcacca ctcaatgagt tggcggaaaa
      421 caattcattg aaaaaaagag tcctggacaa tcacgttcag gactctttta cttcttttat
      481 tcacttaaca actaacactc tgtgagtgaa atggactctt caatgtgatg ttaggatgat
      541 ttcatggaca cgattt
//
LOCUS       DAPBCS010000173          548 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPBCS010000173 DAPBCS010000000
VERSION     DAPBCS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.189"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>548)
                     /locus_tag="RD521_002962"
     CDS             complement(<1..>548)
                     /locus_tag="RD521_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7772603.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DAPBCS010000174          540 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPBCS010000174 DAPBCS010000000
VERSION     DAPBCS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.190"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>540
                     /locus_tag="RD521_002963"
     CDS             <1..>540
                     /locus_tag="RD521_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDT7772604.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQ
                     AAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNLIEQD
                     HRPVKRRNKFYRSLRTASTT"
BASE COUNT          186 a           97 c          104 g          153 t
ORIGIN      
        1 tcagcaggat gtgattattg tagccgtggg ctactatctt cgttataacc ttagctatcg
       61 tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt tctcatacga cgatttatcg
      121 ttgggtgcaa gaatatggca aactactcta tcaaatttgg aaaaagaaaa ataaaaaatc
      181 cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt
      241 gtatcgagcc atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga
      301 cacacaagca gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt
      361 tgtagtcaca gataaagccc cctctattac aagtgccttt aagaaactaa aagaatacgg
      421 cttttatcaa gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga
      481 ccatcgtcca gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctctaccac
//
LOCUS       DAPBCS010000175          528 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPBCS010000175 DAPBCS010000000
VERSION     DAPBCS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.191"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(5..90)
                     /locus_tag="RD521_002964"
     tRNA            complement(5..90)
                     /locus_tag="RD521_002964"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="RD521_002965"
     tRNA            complement(107..178)
                     /locus_tag="RD521_002965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="RD521_002966"
     tRNA            complement(190..262)
                     /locus_tag="RD521_002966"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="RD521_002967"
     tRNA            complement(280..361)
                     /locus_tag="RD521_002967"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="RD521_002968"
     tRNA            complement(369..441)
                     /locus_tag="RD521_002968"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="RD521_002969"
     tRNA            complement(445..517)
                     /locus_tag="RD521_002969"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAPBCS010000176          526 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPBCS010000176 DAPBCS010000000
VERSION     DAPBCS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.192"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..354
                     /locus_tag="RD521_002970"
     CDS             <1..354
                     /locus_tag="RD521_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDT7772605.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..>526
                     /locus_tag="RD521_002971"
     CDS             355..>526
                     /locus_tag="RD521_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772606.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAPBCS010000177          499 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPBCS010000177 DAPBCS010000000
VERSION     DAPBCS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.193"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>499
                     /locus_tag="RD521_002972"
     CDS             <1..>499
                     /locus_tag="RD521_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDT7772607.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSM"
BASE COUNT          164 a          100 c           88 g          147 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatgga
//
LOCUS       DAPBCS010000178          487 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPBCS010000178 DAPBCS010000000
VERSION     DAPBCS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.194"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>487)
                     /locus_tag="RD521_002973"
     CDS             complement(<1..>487)
                     /locus_tag="RD521_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDT7772608.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAPBCS010000179          474 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPBCS010000179 DAPBCS010000000
VERSION     DAPBCS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.195"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          168 a           57 c           67 g          182 t
ORIGIN      
        1 tagaaaaaca acaaattaaa tatataaatt agttaatgtg ttgtgattga ttatttaata
       61 tagatataat acaaaaaggc aaagatattt tcaaaacatt ttttcttgat gtgatatcct
      121 tgtctttttt gtaatgtttt taacttattg cttttgcaaa atttatttac ctgttttctg
      181 gatcaacgat agtgtatatt atgctatcct tgtttggatt ttaggacgtt taaagaggca
      241 cttttttaca taacttaatc catagacctt ttaagcatgc tgtgagataa taataaagaa
      301 gagtttaaag cgcaccccaa accacttccc cataagtgta ttacgcttta aactctttta
      361 tatttgaatt cactaagaag cataccatat ttttgaaaaa aagtgagaaa aaaggcttat
      421 aattaggatt tagagtaatt aattagatgt ttattttata ggactgatac tata
//
LOCUS       DAPBCS010000180          452 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPBCS010000180 DAPBCS010000000
VERSION     DAPBCS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.196"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>452
                     /locus_tag="RD521_002974"
     CDS             <1..>452
                     /locus_tag="RD521_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDT7772609.1"
                     /translation="IINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKI
                     IRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKI
                     HMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          153 a           85 c           77 g          137 t
ORIGIN      
        1 cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt
       61 tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta
      121 cactatcaag attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg
      181 aattatagat agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact
      241 attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct
      301 taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc
      361 aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat
      421 cctaggggac aaaggatata ttagccataa aa
//
LOCUS       DAPBCS010000181          434 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPBCS010000181 DAPBCS010000000
VERSION     DAPBCS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.197"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>434
                     /locus_tag="RD521_002975"
     CDS             <1..>434
                     /locus_tag="RD521_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDT7772610.1"
                     /translation="NLVTAARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSG
                     LVERFNENVNDYNELLEENKSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVF
                     KGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRD"
BASE COUNT          186 a           52 c           91 g          105 t
ORIGIN      
        1 aaacttagtg actgctgcga gagataacga taggttaaaa cagcatgtta gaaatctcat
       61 gagtactgat atggcgagag aatataaaaa attaagtaaa gaacatgggc aagttaaaga
      121 aaaatatagt ggtcttgtag agcgatttaa tgaaaatgta aatgattata atgagttgct
      181 tgaagaaaac aagtctttaa agtctaaaat aagcgattta aagcgtgatg tgagtttaat
      241 ctatgaaagc actaaggaat tccttaagga acgtacagac ggcttaaaag cctttaaaaa
      301 cgtttttaag gggtttgtag acaaggtaaa ggataaaaca gcacaattcc aagaaaaaca
      361 cgatttagaa cctaaaaaga acgaatttga actaactcat aaccgagagg taaaaaaaga
      421 acgaagtcga gatc
//
LOCUS       DAPBCS010000182          424 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPBCS010000182 DAPBCS010000000
VERSION     DAPBCS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.198"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..421
                     /locus_tag="RD521_002976"
     CDS             <1..421
                     /locus_tag="RD521_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772611.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          159 a           65 c           86 g          114 t
ORIGIN      
        1 atcactagat cgtttaggaa gagattatga agatataaaa aatacagttg cttttatgaa
       61 acagaaaaaa gtagcgttaa aaatattaga tgcacagttt ttagatttta atactggaaa
      121 tgaactcctg gataccgcta tgttcgatat gtttttatca ctgctaagtt atattgcaca
      181 aaacgagcgt gagaaaattc gtgaacgtca gcggcaagga gtattattgg ctcaaaaagc
      241 cggccgatat aagggacgac caatcgagta ttctattaac tcaacagatc cgcaaaaacg
      301 cttagttttt aaaaccgtgg tggacatgtt aaagcaaggc attccagttg cagagattgc
      361 aaaagaaaac gggatctctc gaccaactat ttataaaata aagaaagcga atgatttgta
      421 gaaa
//
LOCUS       DAPBCS010000183          420 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPBCS010000183 DAPBCS010000000
VERSION     DAPBCS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.denovo.199"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            74..403
                     /locus_tag="RD521_002977"
     CDS             74..403
                     /locus_tag="RD521_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDT7772612.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          162 a           49 c           76 g          133 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
//
LOCUS       DAPBCS010000184        23728 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.046, whole genome shotgun sequence.
ACCESSION   DAPBCS010000184 DAPBCS010000000
VERSION     DAPBCS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.046"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..212
                     /locus_tag="RD521_002978"
     CDS             <1..212
                     /locus_tag="RD521_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDT7772613.1"
                     /translation="ELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSST
                     HRGPSELCGNVFKIEYTKKRQRLAY"
     gene            447..683
                     /locus_tag="RD521_002979"
     CDS             447..683
                     /locus_tag="RD521_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772614.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            690..2642
                     /locus_tag="RD521_002980"
     CDS             690..2642
                     /locus_tag="RD521_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDT7772615.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="RD521_002981"
     CDS             complement(2714..3211)
                     /gene="dfrG"
                     /locus_tag="RD521_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDT7772616.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3573..4613
                     /locus_tag="RD521_002982"
     CDS             3573..4613
                     /locus_tag="RD521_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDT7772617.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4711..4899
                     /locus_tag="RD521_002983"
     CDS             4711..4899
                     /locus_tag="RD521_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDT7772618.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4957..5310)
                     /locus_tag="RD521_002984"
     CDS             complement(4957..5310)
                     /locus_tag="RD521_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772619.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5841..6263
                     /locus_tag="RD521_002985"
     CDS             5841..6263
                     /locus_tag="RD521_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDT7772620.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6260..6490
                     /locus_tag="RD521_002986"
     CDS             6260..6490
                     /locus_tag="RD521_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDT7772621.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            6988..7188
                     /locus_tag="RD521_002987"
     CDS             6988..7188
                     /locus_tag="RD521_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDT7772622.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7215..8408
                     /locus_tag="RD521_002988"
     CDS             7215..8408
                     /locus_tag="RD521_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDT7772623.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8476..10041)
                     /gene="guaA"
                     /locus_tag="RD521_002989"
     CDS             complement(8476..10041)
                     /gene="guaA"
                     /locus_tag="RD521_002989"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDT7772624.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10100..11023)
                     /locus_tag="RD521_002990"
     CDS             complement(10100..11023)
                     /locus_tag="RD521_002990"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDT7772625.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11219..11824
                     /locus_tag="RD521_002991"
     CDS             11219..11824
                     /locus_tag="RD521_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7772626.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12132..13148
                     /locus_tag="RD521_002992"
     CDS             12132..13148
                     /locus_tag="RD521_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDT7772627.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13289..14590
                     /locus_tag="RD521_002993"
     CDS             13289..14590
                     /locus_tag="RD521_002993"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDT7772628.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14636..15298
                     /gene="deoC"
                     /locus_tag="RD521_002994"
     CDS             14636..15298
                     /gene="deoC"
                     /locus_tag="RD521_002994"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDT7772629.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15319..15717
                     /locus_tag="RD521_002995"
     CDS             15319..15717
                     /locus_tag="RD521_002995"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDT7772630.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15783..16865
                     /locus_tag="RD521_002996"
     CDS             15783..16865
                     /locus_tag="RD521_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDT7772631.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17077..18639
                     /locus_tag="RD521_002997"
     CDS             17077..18639
                     /locus_tag="RD521_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDT7772632.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18632..19765
                     /locus_tag="RD521_002998"
     CDS             18632..19765
                     /locus_tag="RD521_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7772633.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19765..20721
                     /locus_tag="RD521_002999"
     CDS             19765..20721
                     /locus_tag="RD521_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDT7772634.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20820..21986
                     /gene="deoB"
                     /locus_tag="RD521_003000"
     CDS             20820..21986
                     /gene="deoB"
                     /locus_tag="RD521_003000"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDT7772635.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22010..22828
                     /locus_tag="RD521_003001"
     CDS             22010..22828
                     /locus_tag="RD521_003001"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7772636.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22845..23552
                     /gene="deoD"
                     /locus_tag="RD521_003002"
     CDS             22845..23552
                     /gene="deoD"
                     /locus_tag="RD521_003002"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDT7772637.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         7720 a         4202 c         4949 g         6857 t
ORIGIN      
        1 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
       61 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
      121 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
      181 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
      241 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
      301 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
      361 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
      421 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
      481 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
      541 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
      601 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
      661 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
      721 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
      781 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
      841 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
      901 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
      961 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     1021 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     1081 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     1141 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     1201 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     1261 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     1321 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     1381 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     1441 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     1501 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     1561 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     1621 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     1681 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     1741 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     1801 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     1861 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     1921 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     1981 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     2041 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     2101 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     2161 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     2221 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     2281 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     2341 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     2401 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     2461 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     2521 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     2581 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     2641 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     2701 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     2761 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     2821 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     2881 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     2941 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     3001 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     3061 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     3121 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     3181 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     3241 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     3301 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     3361 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     3421 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     3481 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     3541 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     3601 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     3661 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     3721 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     3781 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     3841 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     3901 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     3961 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     4021 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     4081 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     4141 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     4201 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     4261 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     4321 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     4381 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     4441 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     4501 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     4561 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     4621 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
     4681 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     4741 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     4801 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     4861 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     4921 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     4981 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     5041 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     5101 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     5161 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac
     5221 ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac caacggctct
     5281 aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat tttactgttc
     5341 ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt tcctatagaa
     5401 cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg ctgattttat
     5461 ttacctatac aactttataa aatttagtca tactcctaga aagtagataa aattggcgac
     5521 ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct cttaattaag
     5581 ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa tgtggttttg
     5641 cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct gtttatgtgc
     5701 ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca acaaatcccc
     5761 cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact ccctttcacg
     5821 acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata gaaaatcagt
     5881 ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc aaacatctag
     5941 acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt gtcaactctt
     6001 tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt ggattaagaa
     6061 tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag aaaaaacgga
     6121 acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg gagttactaa
     6181 atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg attaaagaat
     6241 tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc ccctcattgt
     6301 aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt atcgagggta
     6361 tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc atatggttat
     6421 tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt tatcgttcga
     6481 aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc ttcccgattt
     6541 gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga taggctaaca
     6601 agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt aagaaaagag
     6661 ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt agtaattcga
     6721 aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga tactcccgta
     6781 tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca tgagaaccat
     6841 gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga tattcttctc
     6901 ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt ggagttttcc
     6961 tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga tttagttgag
     7021 ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga attgatgatt
     7081 acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg tgaagctgtt
     7141 gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt tttgcacagt
     7201 aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga acctattatt
     7261 ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat cgtagtggat
     7321 tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg aaaccagaag
     7381 aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa attttccttc
     7441 catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca actattaaga
     7501 agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt gatgtgcaaa
     7561 attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct gttcaaggac
     7621 tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca aatccatcta
     7681 agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca aaagaagaat
     7741 ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt ttatttatct
     7801 ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc attcaatggg
     7861 aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat tacaagaatc
     7921 aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat attgtattag
     7981 ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat ctaattcaga
     8041 cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta tcatatgcta
     8101 ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg ttacggcatt
     8161 ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa gatcgtctag
     8221 gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct aacagtaatt
     8281 tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa caaaattatg
     8341 atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa atgaaaaaag
     8401 tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat aagaggattt
     8461 ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag tgatgtcata
     8521 cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt tttgcaagac
     8581 ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta cagcacggat
     8641 accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc ggatccctgg
     8701 aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga tttcttcacg
     8761 caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt cgccaagaac
     8821 acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat caggcatgcc
     8881 taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg gttcgatcaa
     8941 ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga tcgtctgtgc
     9001 tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta ggaaagagac
     9061 gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga attcattccc
     9121 aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta agaagcgatc
     9181 tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt ccattacttg
     9241 ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca attggtcacc
     9301 gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg aaagaccaag
     9361 caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat cgataaagtt
     9421 atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc gcagcaattc
     9481 gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa aattacgtgt
     9541 tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt caaagccttc
     9601 aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt ttggtgtttc
     9661 tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat attcgcggtt
     9721 tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac catagcaaat
     9781 ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg cattctcatc
     9841 atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg cttttacttc
     9901 ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac gaatacgtct
     9961 tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt caacgttcgt
    10021 catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta tctcaaaata
    10081 gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg atatgatttt
    10141 cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat tcctgtaaat
    10201 ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct tcacgatctc
    10261 cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc agtaacgcac
    10321 caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca tagacagaaa
    10381 aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc gtattgatcc
    10441 cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga atgatatcat
    10501 acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt acacggttca
    10561 agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg tccatgattc
    10621 cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc agctgaacgt
    10681 ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt gtactttttc
    10741 caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg tatttatgaa
    10801 gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga taaatcagat
    10861 gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca ttcaaacttt
    10921 tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa aattctcgcc
    10981 attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc tcctgcgttc
    11041 tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact taagagagat
    11101 tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt cttgacttgc
    11161 gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga tggaagacat
    11221 gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac aatgggcttt
    11281 tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt cacgtgaaac
    11341 agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt tacctgatga
    11401 aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt tggcatttgc
    11461 gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc tagcgcaagg
    11521 aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata tctatgaagc
    11581 agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg ctggaaaaaa
    11641 agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg gtacactgac
    11701 aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg aagaaacatt
    11761 tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa gtgtgaaaga
    11821 ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc atgtacgggt
    11881 acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt ttttcttcaa
    11941 aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag atacagaact
    12001 tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa catactttcc
    12061 ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt agatagaagg
    12121 gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc gtatcagtaa
    12181 caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag acaagaaaaa
    12241 aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct tcaaatgtga
    12301 tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat ttcttcttct
    12361 ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg atcatgttat
    12421 gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt tcccaccgct
    12481 cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat gcattagaca
    12541 gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat cgtcgggata
    12601 acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg ctgtttattg
    12661 aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat caattagaag
    12721 aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt aatgggaaat
    12781 ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa aaattactca
    12841 aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc ggatgctatc
    12901 aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata ggattcgatg
    12961 gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag ccaagttttg
    13021 atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct caaagaaaag
    13081 ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa gtattgacaa
    13141 agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt tagtgctttt
    13201 tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat aatgtataat
    13261 accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg aaaaaaagag
    13321 ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact atacgaatgg
    13381 aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc aagacatgac
    13441 aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg tcatcgattt
    13501 gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg gtgacacgac
    13561 cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta aaatgtctgg
    13621 acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc ccggatttca
    13681 gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag tagcggtaat
    13741 tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc gtgacgtcac
    13801 agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa aaatcgcggc
    13861 aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca tgaagaacat
    13921 cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg ctaaccgaga
    13981 aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg gaaatagtct
    14041 agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc tagtagaaat
    14101 gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga caaccgatga
    14161 agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa aattcaaaga
    14221 aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac gaattttgac
    14281 tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa aaatcgtggc
    14341 aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca aagaagaaga
    14401 tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg tgacaaaagg
    14461 tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca ttgaattatt
    14521 atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc atgacatcat
    14581 cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa aagaaatgga
    14641 actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag aagctgtgat
    14701 gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca atccagcatg
    14761 ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta cagtaatcgg
    14821 gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag atgccatcaa
    14881 taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat caaaggatga
    14941 tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc gtgcgcttgt
    15001 caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag cttgcgaaat
    15061 agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa caggcggagc
    15121 aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg gtgtcaaagc
    15181 ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg gtgctacaag
    15241 aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag gctattaatt
    15301 ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac agctattgaa
    15361 gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg tctcgtaacg
    15421 gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg attaacgaat
    15481 tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac atttacgcat
    15541 ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg tcgacaagta
    15601 atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa tggtgtgacg
    15661 aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga tctataactc
    15721 atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga ggctttacag
    15781 aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca gggttatttt
    15841 taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc tcatcagaca
    15901 caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca ttcaaccagt
    15961 cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga ggaaatgatg
    16021 gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt gaccaagcat
    16081 tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca gcgatccaag
    16141 aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc atcgatggat
    16201 tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt gcaggtgttg
    16261 cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta aaaggtgaag
    16321 taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg aaagaattag
    16381 gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca gataaaggac
    16441 gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat gcttcaggcg
    16501 gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc gacaaaaaag
    16561 tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca ttaaacggcg
    16621 aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta gtagaagatt
    16681 tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt tatggattga
    16741 aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa aaagcagttg
    16801 atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact cctgaagaaa
    16861 actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg gtttgattag
    16921 aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg gaacaactcc
    16981 ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt agtcaatcgt
    17041 tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa tattgtcata
    17101 gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa catcaattta
    17161 caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg caaatctaca
    17221 ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt gatgcgtggg
    17281 aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg gatggtccat
    17341 cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt gggaagcgag
    17401 cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca aaaagtttct
    17461 gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc agttggaatg
    17521 gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact gatttttgat
    17581 gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat catgaaggaa
    17641 ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga aatcaaagca
    17701 gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt caacgttaaa
    17761 gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc attcaaaacg
    17821 atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct agtggtgaaa
    17881 gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc tggcgaagta
    17941 cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc tttgactggt
    18001 ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac taataaaaaa
    18061 ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca taaatacggg
    18121 ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca tcaaaaacct
    18181 tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag agaattgatc
    18241 gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt atcaggcgga
    18301 aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct gttgattgtt
    18361 gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa acgtctgatc
    18421 gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga agaaatttta
    18481 aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat cgttgatccg
    18541 aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt agaagaagca
    18601 cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa aattccgtaa
    18661 tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga tattgatggc
    18721 tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag cccttggttc
    18781 gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta cagcattagg
    18841 tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac aagcactttg
    18901 cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa aagttgtttt
    18961 gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg ctgttccagg
    19021 tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga tgaactatat
    19081 cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct atcgctcaag
    19141 tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat ggttaacaca
    19201 agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg cgcttatttt
    19261 cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg ttggattgaa
    19321 tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt tgtcgatggt
    19381 catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa cgtatcaaaa
    19441 cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg tttccttgct
    19501 tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta ttttgaagat
    19561 cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca atgtatcgat
    19621 tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca tggctaaagt
    19681 cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac caacgaatca
    19741 aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt gatccttact
    19801 tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac tttttctgaa
    19861 cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc ttttagttcc
    19921 atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg gatcgctatt
    19981 ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc aacagtcagt
    20041 ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc tgctttaggt
    20101 gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac gcagaatctg
    20161 gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat tttctttaaa
    20221 aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg ggtgatcatc
    20281 ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca agctgccgac
    20341 acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc tggcttgtta
    20401 ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc agctggtacg
    20461 atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg gaatccttta
    20521 ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat cattggcgca
    20581 caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc atatttgctg
    20641 acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc aaacgggaag
    20701 aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac gggaagtgtt
    20761 tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa ggaataatca
    20821 tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa gcaccagatg
    20881 ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa gaagcaggat
    20941 tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt gaaggtgtgg
    21001 aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct gtagggaaag
    21061 atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca tttcgtgtgt
    21121 ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct ggccgtaaag
    21181 tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt gaacaccaaa
    21241 tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag atcgcggcac
    21301 atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt cgtgatataa
    21361 caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg ggtgaaccag
    21421 gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc ggacacacag
    21481 tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag atcaacgata
    21541 ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg gatggcgttg
    21601 accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc aatttagtag
    21661 attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat gcgattgaag
    21721 aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac ttgctaatga
    21781 ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg cgagaatatg
    21841 taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct caaggatatt
    21901 attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact gaaaatggcg
    21961 aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa tgaccaagtt
    22021 aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa aagaaattga
    22081 ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg aagatgctgt
    22141 aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg ggcatgcagg
    22201 aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag ggagattcca
    22261 ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga tggccggact
    22321 aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat catttgtacc
    22381 aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc ctttgatcgg
    22441 tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt atacgcctga
    22501 ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta aagaaggcgt
    22561 atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa tgtctcggac
    22621 aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag cagctcacag
    22681 cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta tgcaagcgaa
    22741 tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt ttaaagcatt
    22801 ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt gtgcatattg
    22861 aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct ttgcgagcta
    22921 aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta cgcggtatgt
    22981 taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca ggaatgggaa
    23041 tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta aagaaattga
    23101 ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat ttagtactag
    23161 cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc tttcattttc
    23221 ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa gaaaagggct
    23281 atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat gatttgacag
    23341 agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca gcggctcttt
    23401 attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt agcgaccatt
    23461 tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat gaaatgatcg
    23521 aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct gccctgcatt
    23581 aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg ctggacttat
    23641 tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct gccgtttaac
    23701 tctgagtcac aaggaacaat ttcaaaaa
//
LOCUS       DAPBCS010000185        20943 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.051, whole genome shotgun sequence.
ACCESSION   DAPBCS010000185 DAPBCS010000000
VERSION     DAPBCS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.051"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(101..544)
                     /locus_tag="RD521_003003"
     CDS             complement(101..544)
                     /locus_tag="RD521_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDT7772638.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINAMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILTSKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(739..2079)
                     /locus_tag="RD521_003004"
     CDS             complement(739..2079)
                     /locus_tag="RD521_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7772639.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGIILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHAADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFAAYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLIIFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLTLRKGTLW
                     DKGISFFALAVLWLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2361..3257
                     /locus_tag="RD521_003005"
     CDS             2361..3257
                     /locus_tag="RD521_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDT7772640.1"
                     /translation="MNKNDVLLIAKNKSLYFFPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMDFSSSEPKTDLFFGATNNGRKERAEKYKQEFTKR
                     GVTTNYFILPLRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYQFYHSENIFLLQERDLDELPEFLHTPFQPIPSELLEFFDAENWAKR
                     FFTTDSAIYEQYEYDLSSVKNK"
     gene            complement(3400..4341)
                     /locus_tag="RD521_003006"
     CDS             complement(3400..4341)
                     /locus_tag="RD521_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDT7772641.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVVLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNKHVDLIVALTKANQLDDMFRSIQSIITENTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEAKLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAEGM
                     VRTIVSEFASIAAKEGITLDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAVWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4467..5588
                     /locus_tag="RD521_003007"
     CDS             4467..5588
                     /locus_tag="RD521_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDT7772642.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALGGTWRTIDGMDLLFRQRKISYLLTNWQGKNTAVIESAQVLGLDLLDKP
                     TDQTIRLASSYALGELIKDTLLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTRKLHLDRRNVLVSKVKVIIAADVTNPYTGQQGAAWQFGRQKGGTDTTLAFLD
                     KQAEKIAQQIKDVWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFELVSQITGLEKQI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDRELEGLFDFLPIV
                     LSIQLGPIDLEEAMEHRQTLEKTEILGQTLSRFFNILPK"
     gene            5846..7348
                     /gene="eat(A)"
                     /locus_tag="RD521_003008"
     CDS             5846..7348
                     /gene="eat(A)"
                     /locus_tag="RD521_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDT7772643.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQAFVYFPQQTKDKERLTYYVLNEIMDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEIAKNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKKVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDALTMNSQVSHHKRLLSVENLQLGYENLLFEPVHF
                     TIEPHQRMAISGPNGAGKSSIIHYLLGTFNGKVVGEKSQPKHLTISYVRQNYEDNRGT
                     LAEFAKKNRVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYI
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLEKI"
     gene            7376..7942
                     /locus_tag="RD521_003009"
     CDS             7376..7942
                     /locus_tag="RD521_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDT7772644.1"
                     /translation="MVKENFVNEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQALAEKVVDLPVPITKIIHSPLQRARDTAQAVVDRLNLPMTADERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECMEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELISYE"
     gene            8154..9215
                     /locus_tag="RD521_003010"
     CDS             8154..9215
                     /locus_tag="RD521_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDT7772645.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQNRLIELGSEAKKNKLELMVDISGEALKRAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPNDIDELKEAAADFSQIEAWHNYYPRLETGLDKEWYQEK
                     NQWLKTQGFLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSEHVQEQFASFQKEQTVLLHTEPVDEVFYEYILGKHTNRQDDARDVIRSADARF
                     REIPPIPVRNTAKRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9218..10114
                     /gene="murQ"
                     /locus_tag="RD521_003011"
     CDS             9218..10114
                     /gene="murQ"
                     /locus_tag="RD521_003011"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDT7772646.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELPSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISEHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEAAQAKLAEANGFVKGTL"
     gene            10128..12110
                     /locus_tag="RD521_003012"
     CDS             10128..12110
                     /locus_tag="RD521_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDT7772647.1"
                     /translation="MAKNELTLEELAKQIYQGAGGMGNVESIVHCMTRVRMKVINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQEMVDLAGVKLGEKLPSASATTAS
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAGTWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALATGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKAVPQASA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVISSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAADK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="RD521_003013"
     CDS             complement(12180..12626)
                     /gene="nrdI"
                     /locus_tag="RD521_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDT7772648.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEYEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQRFGFPFLADYELRGTPSDVTRVYQILAEKNK"
     gene            12837..14261
                     /locus_tag="RD521_003014"
     CDS             12837..14261
                     /locus_tag="RD521_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDT7772649.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNVASIVMSTIFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFINNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGFLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14438..15211)
                     /locus_tag="RD521_003015"
     CDS             complement(14438..15211)
                     /locus_tag="RD521_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDT7772650.1"
                     /translation="MTLLIKQELFKLIKKKSTAVLSVLLVALLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMESQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSIYLYLLAIIVTVLMKLILFPSISFTEKISTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIALFLALGYLVFKRKNV"
     gene            complement(15208..16113)
                     /locus_tag="RD521_003016"
     CDS             complement(15208..16113)
                     /locus_tag="RD521_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDT7772651.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSEGTIRIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVKQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDQAARTILTDAGYPLEDPDKIKLLKKNEELLGTVIQLLSAENVHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16274..16594)
                     /locus_tag="RD521_003017"
     CDS             complement(16274..16594)
                     /locus_tag="RD521_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDT7772652.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGLLLISIIGIKI
                     TSGH"
     gene            17067..17753
                     /locus_tag="RD521_003018"
     CDS             17067..17753
                     /locus_tag="RD521_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDT7772653.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17755..18699
                     /locus_tag="RD521_003019"
     CDS             17755..18699
                     /locus_tag="RD521_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDT7772654.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18898..19542
                     /gene="pcp"
                     /locus_tag="RD521_003020"
     CDS             18898..19542
                     /gene="pcp"
                     /locus_tag="RD521_003020"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDT7772655.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19621..20898
                     /locus_tag="RD521_003021"
     CDS             19621..20898
                     /locus_tag="RD521_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772656.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6757 a         3774 c         4370 g         6042 t
ORIGIN      
        1 cacaacctct gattcacggt gttccagaag tagcttcttc ttatttcttg aagttgatca
       61 cttctggaac atcctctttt tttatttgga tggataatgg tcattccatt tctttgatca
      121 tttttttcat tttgtcgatt tcttcttctt gtgcctgaat gatttcttcc gctaattttt
      181 ttacttcagg atcttttaaa tcagctcgtt tacttgtcaa gatagcaata gagtgatgag
      241 ggatcatcgc cttcatccac gccgtatcgt tgacagttgt ttggcttcgt accataaata
      301 aggaaccggc aaaaacaaga agactagcgg ctaaaatcat agcatttatc tttttgtttt
      361 catacatttt ccacatgaac aacaacatga tgactgccat gactgcaccc atcatcaacg
      421 ccatgaatac acgtgtgcga ctgaagaaga tatgatcaag cgcataaaca ttcaaataca
      481 tcataccaaa catgactacc gtcgaaacac caatcatcaa caaaaacttc caatactttt
      541 tcatttaaaa caaactcctt tttcttcatt cctttcttct atcatgtcat gagcgtggag
      601 aacaatcaat taatctgcac agatggtttt tgaacaccgt ttattcggaa aaaagaactg
      661 caacaatttt tgtcgcagct cctttttttc aatagctcca gatattcaat ataaatagaa
      721 taaaattctc tattggtttc attcattcgt caagacgaaa gcatcattct ttttttgata
      781 actgaaataa atgaacaggt aaagcaaacc ggtcaataca gttaccccaa tggagttcgt
      841 gaataggata ccaaggaaaa tcaaccataa gactgctaat gcaaaaaagc tgattccttt
      901 gtcccataac gttcctttcc ttagtgtcag tacaaaggac gcagcactga taatgatcca
      961 gatcaataat actgtatagg aaagagaacc tgccagatag ccaaataatt tgtcaccgac
     1021 aaaataagaa agagccacac ctagataaag aacactggta caaaacagca cagcacgttg
     1081 cggtacttga tgcttgttta aaacagctag ttttctagag gaacctttcc gatctttcaa
     1141 gcggaagaac aaaattctcg aagaagcata tacacctgaa ttgatagacg agaacagagc
     1201 aagaacaatc acgaaattga cgatatctgc cgcaaatgga atattcattt tttcaaatac
     1261 catcacaaaa ggactaacgt tcgtgcctgc taaagtattc caaggaaaaa taatcaaaag
     1321 caaaaataaa ggaataatgt aaaaggaaat gattcttccc atgacgcctt tgattgcttt
     1381 aggaatcgca cgtttaggat catctgcttc gcttactgtg atagcaatca actcagaacc
     1441 gccgtaagaa tagataacta caagcaaaga actgatgatt cccttcatac cgtggggagt
     1501 aaaaccacca tgatcagtta attgttgaag ggtagggaaa acaccagtac ccaacatttg
     1561 ctgaccgaca agataagcag caaaaatgat caataggata atgacactga ttttagcaaa
     1621 ggccagccaa tattctgtct ctgcgaataa tcgtaccgaa taaagattga tgatagtagt
     1681 taaaacagcc aacataaaaa caaataccca agctgggatg tgggggaacc acaactgtaa
     1741 aaaggaagca gctgcaacag cttcagcaat gatattgata atccacatgg accagtagac
     1801 ccaatcggta aaatcggcag catgagcacc tagatatgga cgaatcaatc cagataatcc
     1861 gtggtgcgta tcaccaccac ttaaaacaat ttttcccaga cctttcatca cgatatataa
     1921 aattatgccg ccaatcaaaa aagcgatcaa tacagaaggg ccggcgattc caatcgcttc
     1981 gccacttcct ttaaatagac ctgcaccaat cgctccacct aaagctagca tggtgatatg
     2041 tcgggagcta agttgttttt tcaatttttt atcttccata aataacctct ttcttttaaa
     2101 ttcacaatca aaatgcatag taagaataaa tagtaagaat aaatagtaat aataataatg
     2161 actaatgata acaaattttc agataattac aactattcag aaaacttttg ggcatatttt
     2221 aatggaagaa tgatttattt catcgtattt tcatagaaat gttaaatttt tataaaaata
     2281 ataggaagcg ttttactggc tttttactat ttaaacaggc tatcatagga aaaagtgaca
     2341 gagaaagtga ggcggaacag atgaacaaaa acgacgtatt gctaattgcg aaaaataaat
     2401 cactttattt ttttccttcc atccgctctt tagatgtttg gtccggaaaa gatttactac
     2461 agtcggaatt cattaaatca gctcgacaac agttggcaaa ttatcagctg atcgtttttt
     2521 tagattatgg atttgatatg gagatggctc gacgcgtgcg tccatttacc aatgcaaaaa
     2581 tcgtgttatt tttttggaac cactttaaag cagaacacga ggcgatgctg aagcaggcac
     2641 aagatgaacc ggcaatcgat gagatttatc attttgattt tttagaagcg aaagaacttg
     2701 gactaaaaca taatagttcc ttttattcga aacatatgga tttttcttct agcgaaccaa
     2761 agacggatct attttttggt gcaacaaata acggcagaaa agaacgggca gaaaaatata
     2821 agcaggaatt tacaaaaaga ggtgttacga ccaattactt tattcttccg cttcgaggaa
     2881 atgaacaacc tggatacctt acttatgaag aatatctgaa aaaaaccggg gaatcgaggg
     2941 ggattctaga actgttgcgg gaagggcagc aaggagtgac tttgcgaacg tttgaatcta
     3001 tgtattttca aaagaaacta gtgacagata atcaagccat agctcattat cagttttatc
     3061 attctgaaaa tatttttttg cttcaagaac gtgatttaga tgaattaccg gaatttttgc
     3121 atacgccatt tcaaccaatc ccatcagaac tgttggaatt ttttgatgca gaaaattggg
     3181 caaaacgttt tttcactact gactccgcta tttatgaaca atatgaatac gatctttcct
     3241 ctgttaaaaa taaataaaag tgccaaccaa tcatagatga aaataaacaa gcgcaattct
     3301 tatggcaaca tgagaattgt gtttttcttt ttgaactttc atgggggtaa aaaaatgaga
     3361 accagctgct gatccagctg attctcatca gagagccttt tattctgcgc caaggattcc
     3421 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     3481 ttgtcctttt cgccaaacgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     3541 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     3601 agcaatatgt tggtacactt cttcttgatc taatgtgatt ccttcttttg ctgcgataga
     3661 agcaaactca gatacaatcg ttcgaaccat gccctctgcc gctttttgcg cacctaattc
     3721 agcaatatta caatccaata tcgtgcataa tccatttaat gttccgttta cacaagcttt
     3781 tcgccaaatg gaataacgaa cattatgact atagattgga ttcaattttg cttcttgaaa
     3841 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     3901 ttctccatca cctaataatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     3961 gatcccaaat aagatatttt tctttggtac atatttttcc agtacatctt catgacctaa
     4021 tccattcagt aaacacaaaa cataagtatt ctcagtaata atggattgga tcgaacgaaa
     4081 catatcatct aattgattcg cttttgtcaa ggctacgatc aaatccacgt gtttgttgct
     4141 ttcgatgatt tcttctggtg aatagattgg cagttttgca actacttctt tgccattaaa
     4201 gtcagcaatc aatccatttg tacgaatggc ttcaatgtga gcaggccagc gatcgatcaa
     4261 gacgacctca tttccgcttt gatgcaacat taatccgatt cggctgccca ttgctccggc
     4321 tccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     4381 tatagaaaaa cagaagaaaa tagcattgtt ttgtttgaaa aaagtgaaaa aaagtgtctt
     4441 acttaagaag aaagtgaggg atatagatgg atattttggc ggcaatcgat tcatttaaag
     4501 gaagtgcttc ttcatttgaa ttaaatcagg cagttctaaa tggttgggaa gaccgtaaag
     4561 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     4621 acgcgctcgg gggaacctgg cgtacgattg atgggatgga tctcttattt cgtcagcgaa
     4681 aaatcagtta tttattgacg aactggcaag gaaaaaatac cgcagtcatt gaatctgcac
     4741 aagtattagg attagattta cttgataaac caacggatca aaccatccga cttgcttcta
     4801 gttatgcctt gggagagctt atcaaagata cactgcttca agaagtggaa caaatcattg
     4861 tcactttagg aggaagcgga tgtagcgatg gtggtctagg attactccaa tctttgggtg
     4921 cttctttaga agggtacaca gaaggaaatc ctttgttatc tactagaaaa cttcatttgg
     4981 atcgaaggaa cgttcttgtt tctaaagtaa aagtgatcat cgctgcagac gtgaccaacc
     5041 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaacggata
     5101 cgacgttagc tttcttggat aaacaagctg aaaagattgc tcagcagata aaagatgttt
     5161 gggaggtgga tttagatgac atcccaggaa gcggagcagc aggcggtatt ggcggagccc
     5221 tttttttgtt aggagcgaag atggtctctg gttttgagtt ggtcagccaa atcactggat
     5281 tagaaaaaca gatcgaacaa gcggatgtca tcattacagg agaagggcgg attgatcagc
     5341 aaacaatcca cggaaaagtt ccatatggcg tagcagtgct tgcaaaaaaa tatcgaaaac
     5401 caataatcgc tatttgcgga agtagagacc gagagctgga aggactcttt gattttctcc
     5461 ccattgtctt aagcattcaa ttaggaccaa tcgatttaga ggaagcaatg gaacacaggc
     5521 aaacattaga aaaaacagaa atcttaggac agacactcag cagatttttc aacattctcc
     5581 cgaagtagtt gacaaaaaca atttctttat gctatcgtaa tttcaaatat aggacaaagt
     5641 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     5701 tgtggagaca agggagcagt gtgcagattt tcgggattgg cttggttctt tcgatgtatg
     5761 ggtctttttg tgtgccccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg
     5821 tcttttttta cgaggtgatg gaataatgtc taaaatcgaa ataaaaaatc tgacattcgg
     5881 ctatgacagc caaggcacat tattatttga acaagccaat ctaaactttg acacacaatg
     5941 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atatcctgca
     6001 aaacaaactc ccttatcaag ggcaagtgat ccatcagcaa gcgtttgtct atttcccgca
     6061 gcaaacaaaa gataaagagc gtttgaccta ttatgtgtta aatgagatta tggattttga
     6121 gatatgggaa attgaaagag agctccaact gatgcaaaca gatcctgaaa tcttatggag
     6181 agaattcagc acactatcag gaggagagaa gacaaaagtc ctactggcac tcttatttgt
     6241 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggcag
     6301 aaaacaagta gcggcttact tgaaaaagaa aaaacaagga ttcatcgtgg tgagtcatga
     6361 tcggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaga gccaactgga
     6421 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     6481 aatagctaaa aatgaaaaat tgaaaaaaga agtcagcagg ctaaagaaaa cagcagctga
     6541 aaaagcagaa tggtctcgtt cccgagaagg ggacaaaaca aagaaaaaag tcggattcat
     6601 cgatacggaa tctaggcgag tgaataaagg agcagtaggc gctgatgctg cacggacgat
     6661 gaaacgatct aaagcaatcg tgaatcggat ggaaacccag atcagtgaga aagaaaaatt
     6721 attaaaagat atcgagtata tcgatgcttt gacgatgaat agccaagtgt ctcatcataa
     6781 acggctgtta agcgtagaaa atcttcaatt agggtatgaa aatctgttat tcgagccagt
     6841 tcattttacg atcgaacctc atcagcggat ggcaatttca ggtcctaacg gcgcgggaaa
     6901 gtcatccatt atccattatc ttctggggac attcaacggc aaggttgtag gagaaaaatc
     6961 ccagcccaaa catttgacca ttagttacgt acgccaaaat tatgaagaca atcgagggac
     7021 attggcggaa ttcgcgaaga aaaaccgagt agactatcaa gcatttttga ataatctccg
     7081 aaagcttggg atggaaagag atgtttttca taataaaatc gaacagatga gtatgggaca
     7141 acggaaaaaa gtggaattgg ctaaatcttt atcccaacca gctgagctct atatatggga
     7201 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgattttgaa
     7261 cgtgaaacct gctatgttac tagtggaaca tgatcaaacc tttctagata aagtatctac
     7321 tgagattatt tctcttgaga aaatctaaac cttgtaatct caaagacaac aaggtatggt
     7381 taaggagaac tttgtcaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     7441 aacaacttgg aacgcacaag gattagtttg cgggcgagca gatgtgccgc tgactgaaaa
     7501 aggacagatg caggcacagg cactagcaga aaaggtagta gatttgcctg ttccaatcac
     7561 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag tagttgatcg
     7621 tctcaatttg ccgatgaccg ctgatgagcg attggtagaa atggattttg gggattatga
     7681 cggactgcca agcaaagatg aaaactttca aaaagcgcgt ttagcgtttg ctgtacgttt
     7741 cccaaatgga gagtcggtct tagatgtgta cgcacggatc gttcctttat tgaaagaatg
     7801 catggaagac gaagaaaatg tctacttgtt agtctgtcat aatgccttga taagagtgat
     7861 caatgcttat tttcatccaa tgcctaatga agggttcttt acattcatgg tagacaatac
     7921 agaattaatt tcttatgagt aattagactt ttgtgttatt taaataaaat gtttcaaggc
     7981 actgcttttg cttagcagtg ccttttttgt ggctgtgtca attcttttgg cattataaat
     8041 ccaattttta gaaataaaat ttaattattt taacaaaagt attgaaattt tgtttcttta
     8101 gatatatact ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg
     8161 gcttttcaat atttatgaat gattctttgt cagaagagaa gaaacagtat atccgccaaa
     8221 tggcatccaa tggctttgtc ggtattttca cttccatgca tataccggaa gatgatgttt
     8281 cggcttatca aaatcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagagt
     8341 tgatggtcga tatttcagga gaggcattga aacgagctgg cttttcatta gaagatctaa
     8401 cacccttgaa agcaattggt gtaacagggt taagaatgga ttatcatatt tctaatcagc
     8461 agatcgccga ctggtcacat caattgaaaa tcagtttgaa tgccagcacg atcacgccta
     8521 atgatatcga tgagttgaaa gaagcagcgg ctgatttctc acagattgaa gcttggcata
     8581 attattatcc acgtcttgaa acagggttgg acaaagaatg gtatcaggaa aaaaatcaat
     8641 ggctcaaaac tcaaggattc cttgtacaag gctttgtacc aggagataca gagttgagag
     8701 gtccactgta tcaaggatta ccaactttgg aagaacatcg agggatccat cctctggcag
     8761 gtgctcttga tctattagca agtaacacgg atattgttta tatcggagat gcaggtctgt
     8821 cagaacacgt tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata
     8881 cggaacctgt agatgaagtt ttttacgaat atatattagg aaaacatacg aatcggcaag
     8941 atgatgcaag agatgtcata cgaagcgcag atgcgcgttt tcgagagatt ccgccaatcc
     9001 cagtaagaaa tacagctaag cgtatgaaag gatctattac tttagataac gaaaagtatt
     9061 tgcgctatat gggtgagatc caattaacta aatatgattt gccagcggat gaaaaagtga
     9121 atgtagtagc caaagtcatc aaagaagaac taccgctgat caaccaaatc aaagctggta
     9181 tgaactatca atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa
     9241 cctaacaacg gaacgcagaa accaagaaac ttttggatta gatgaaatga gtatttctca
     9301 agcagtccaa ctcatgaaca aagaagatca gaaagtggcc gctgctgttg aaaaagaact
     9361 gccaagtatc gagccagtga tcgccaacac gattgaatcc tttaaaaaag gtggtcgctt
     9421 gatttatatg ggagctggaa cgagtggtag acttggtgta ttagatgctg ctgaatgtgt
     9481 accgacattc ggtgtagaac cggaaatggt cataggccta attgctggtg gagaagaagc
     9541 aatgactgta gccgtcgaag gagccgaaga ttcttatgaa ttagggcaag aagatctaat
     9601 caagctgaat ttaacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc
     9661 atatgtgatt ggtggattag attacgcaaa atcgattggt gctgttacag gaacgatttc
     9721 ttgcaataaa caagcagaaa tcagcgagca tgcggatttc ccaatcgaag tcgatgcagg
     9781 accagaattt ttgacaggct ctactcgatt gaagtcagga acagcacaaa aactgatttt
     9841 gaatatgatt tctactatca gtatgatcgg tatcggtaaa gtattcaata acttgatggt
     9901 cgacgtcaaa ccaacaaatg aaaaactaat tgaacgctca aaacgaatca tcatgcaagc
     9961 gacagaagct gactacgaaa cagctgaaaa gtactttgag gaagcagatc aaaatgtcaa
    10021 actggctatc gtcatgattt tgacaaatag tgataaagag gcagctcaag caaaattagc
    10081 tgaagcaaac ggttttgtaa aaggaacctt atgaggggga aaagaccatg gcaaaaaacg
    10141 aattgacttt ggaagaactg gcaaaacaaa tttatcaagg agcaggcggt atgggcaatg
    10201 ttgaaagcat tgtccactgc atgactcgtg tacgtatgaa ggtcatcaat gatgatcttg
    10261 taaatgtctc ggaattaaaa gcgattccag gagtacttgg cgtagtagag gatgaacagc
    10321 tccaagtcat catcggacct gggaaagtga acaaagtagc gcaggaaatg gttgacttag
    10381 caggcgtgaa attaggagaa aaacttcctt ctgctagtgc aactacagca agttcttctg
    10441 catctggaaa agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga
    10501 aacaaaaacc ctctaaaatc aaatcggtat taaaagacat ctctaatatc tttgtaccaa
    10561 tgatccctgc atttgtcggc actgggatcg tggcagggat tgccgcagtt ttagcaaact
    10621 tagtcacagc tggaacacta gacgcaggaa catggcaaca atatatcgat gtgatgaaca
    10681 tcttgaaaaa cgggatgttt agctatctag ttatctatac aggtatcaat gctgcacaag
    10741 tttttggtgc aacgccaacg cttggtggag ttatcggggc tgttgtgatg ctgacaggga
    10801 tgaatccaga agcaccactg aaaaacttgt ttaccggtga agctcttgca actggacaag
    10861 gcgggatctt aggtgttatc ttcgcagttt ggttattgtc aatcgttgag aaaaaactgc
    10921 ataaagtagt acctgatgcg gtagatatca tcgttacgcc aactctttcc ctgattgcca
    10981 tcggtctgat cgaaattttc ttcatcatgc ctttagcagg gttcatttct gacggcatgg
    11041 tcggtgggat caactgggta cttggtgttg gaggagcatt tgccggcttc gttctaggta
    11101 cattattctt gccaatggtc atgttcggac tacatcagat cttgacgcca atccatgtac
    11161 aaatgattga tgaaactggt cgtaccttat tgcttccaat ccttgcgatg gccggagcag
    11221 gacaagtggg tgcagcattg gcattatgga tccgctgcaa aaaagacaaa gaattaacag
    11281 aaatgatcaa aggggcttta ccagtcggaa tcttaggaat cggcgaacca ttgatttacg
    11341 gagtaacatt acctctagga cgtccattta ttactgcttg tatcggtgga ggtatcggtg
    11401 gggcagtgat cggaatgatc ggaaatgtcg gagcaatcgc tattggacct tcaggagccg
    11461 ctttgattcc attgatctct gatggtaaat ggttaggcta tatccttggt ttattggctg
    11521 cttacgctgg tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa
    11581 aagaagaact agcgaccgaa acagccacag taaatgaagt acctgaaaaa gcagtacctc
    11641 aagcttcagc tgaaagtgtg actatcttat catcagttgc tgacggaaca gttgtttctt
    11701 tagataaagc aagtgaccca gtttttgcac aaaaaatgat gggtgaaggt tactttgtag
    11761 aaccagaaaa cggacatatc tattccccgg ttactggagt aataagttct gttttcccaa
    11821 caaaacatgc tatcggcatt acgacaccaa atggcttaga aatcttgctt cacatgggca
    11881 tcaacacagt tgacttaggc ggtacgccat tcgatgtgaa agtaacagaa ggacagcaag
    11941 tgacacctga tacgcttgta gcagaagctg acttagaagc aatcaaagca gcagacaaag
    12001 aaacctccat gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa
    12061 aaacaggtaa agcgaaagca aaagcacctg tgatggatgt caaagcttaa taagaataga
    12121 aacaatagag aacataaaaa gaagaagccg aaatttattt ttcagcttct tcttttgtgt
    12181 tatttatttt tttctgccaa aatttgataa acgcgtgtca catcagatgg tgttccccgt
    12241 aattcataat ctgccaagaa aggaaagccg aatctttgtg cgtattgttt ggctgtcaga
    12301 caatattgat tgttgaagtt tttattaccg ctaccgacta caccaaggca aagtttatga
    12361 ttgtcttcgt attccagata ttcgcgcatc gtttcggtca agatttctgt gtcaccgttg
    12421 tctaacccat tcccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct
    12481 ttcgtaaaat cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct
    12541 tctgcatgca ttttttcagc atattctgct agatgtttgg caaaagcacg cgtatttcct
    12601 gaaatcgata tatataaaat gttcatggat catcccccaa ttctttccct ttagtatagc
    12661 atctttttaa gtttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata
    12721 atgagaaaaa agtcggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat
    12781 aaaaaaggtt tgttatactt tttgataaaa gcggttttac tttggaggat gcttacatgg
    12841 atacagagca acagaaaaaa ggaattggtt tgattccact tgctgcctta gtgattggtt
    12901 cagcgattgg aggtggcgta tttggtatta tgacggacat ctccggatcg gctggtggcc
    12961 cagctatctt cagctgggta ctcgtaggaa cagcaatgtt gatgctctcc ttatcgatca
    13021 ataatatcaa taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag
    13081 gttttggccg tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag
    13141 gaaacgtggc gttcgcaaca ttgttgatga gtgcattagg ttatttcttc cccgtattcg
    13201 aaggtggtca aaatgtggca tcgatcgtga tgagtacgat cttcttatgg ggatatgcat
    13261 ggctagtgaa tcgtggagtt gaaaatgcta gctttgtcaa tgctatcgtc acattttgta
    13321 aattagtgcc attattttta tttattgtag tagctatcag caccttttcg atccaacaat
    13381 tcattaataa cttttctagc gatatcatat tgaatgcgaa tgggcagccg atcccgtttt
    13441 ctagtcaagt attgagttgc atcatggtga tgctctgggt attcgttggt attgaaggag
    13501 cttctgtcgt aggttcacga gcaaaaaaac aatcggatgt tgggaaagca acgattctag
    13561 gaatattcgg tttgatcatt atttacgtct tggtttccat gcttccatat ggcacaatga
    13621 cgatgaatca gctgcaaacg atcaaagcgc aacctgctat gggatatgta tttgaaatga
    13681 tggttggtaa atggggggcg gtcgtgatca acggcggttt gatcatttct ttagttgggg
    13741 tttggctctc atggacaatc ttgccgattg aaacaatggg gaatatggct gaggacggac
    13801 ttttgcctaa agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat
    13861 tgacgacgtt atgtacgaat gtctttttac tttcattatt attcactgac agtgcatata
    13921 actttgctta tacacttggt acagcagcga ttttcttcac gtggcttttc ttagggctgt
    13981 accaaatgaa gctttcttat cagcggaaag agtgggtcca atttttagta gggtttttag
    14041 caagtgcttt tcaagtatgg gccatgctag ttgtagcctt taatgaagtg atgcttgttc
    14101 ttgttctttt cttaccaggt atttattttt acctcaaagc acgtaaagaa caaaacgaac
    14161 ctgtacaaca tatccactta gataagattt tacttatcgg tgtgacgatg gtcggagtaa
    14221 tcgcaatggt tttatttgcc actgggatgc ttcacgtata gaagtataaa gagaggctgt
    14281 gaagcctgtg gtttattcaa aaaagaagcg gtgacaacat tcgtgtcatc gcttcttttt
    14341 ttgaataaat ggtgttcaag aagtagcttc ttctaaaaat aagccgaaat ttcttgaaac
    14401 tggccacttc tgtcacaact tctttgttaa tgaaccatca tacatttttt cgcttgaata
    14461 cgaggtatcc caatgctaag aacaaagcaa tgtaagctaa attgccaaac agcatttgat
    14521 tagtagaaag atgagtcaat tgcttcataa tctcttcgtt gccaatttgg ttttgcagat
    14581 tcagcatact gattggattc catttgatcc actcccattt ttgaatgagt gcgatgagga
    14641 taccagatat gattgatgaa gcaaaataaa agacgatccc tgctgagatg gaggcaccgg
    14701 aactattgat aaaacaagca atcatcaaca ctaaacttaa aattagccat aaaccgatat
    14761 aactacccaa agtataagtg aataagcttt ggatcagtgt ctgaccagta gaaatcttct
    14821 ccgtaaagga aatcgaaggg aaaaggatca gtttcatcag cacggtgaca ataatagcta
    14881 aaaggtataa ataaatagaa taaataacta atgtgatcca tttacttact aatatttctc
    14941 ctcgagaata ctttcgataa aggagatttt tcaatgtacc atattgtgat tccattgaaa
    15001 tgatcgtact tgcagcagca atcatgataa agacaatcca agatgtggca ctgaaaagct
    15061 gagcagtcat ttcaactgga tcaatgatcg tcgtgtattt tttggctaga agtgctgttc
    15121 ctatgagcaa agcaactaat agaacagata aaacagcagt tgactttttc ttgatcaatt
    15181 taaataattc ttgttttata agcaacgtca tttttcttct tcccccttta ctgaatccag
    15241 aatttccaat agggaagatt ccaaatcatt cgtcagatgc tggacatctg tcacatggac
    15301 attttctgca gacaataact ggataacagt tcctaataac tcttcgtttt tttttagaag
    15361 cttgatttta tcaggatctt ccaagggata tccagcgtct gtcaagatcg ttcgagcagc
    15421 ttgatcattt gtcgtagaga taacgatgaa atcttgcgct gtttggttca attcagccat
    15481 tgtacattgt ttgatgattt ttccttgatt gatcaggatc agctcatcga ccattttttc
    15541 aagctcgctg agtaagtgac tggagatcaa gaaagaggtg ccctgagtag ccaagtcggt
    15601 aatgattttt cgcacatctc ttgttgcttg tggatccaaa ccgttcatcg gctcatctaa
    15661 aataacgata gacggatggt tcagtaaggc aagagcaatt cccaattttt gtttcattcc
    15721 cagcgagtag gatttcgctt ttttgtgaat gtatttttcc atcttgagct gtttcacgat
    15781 agtgtcgata gcagatacat cgtttgtttc agagaataac ttcagatgat cataacctgt
    15841 taagaatgga tagatacccg gatattcgat caatgctcct acttgttcta atcctttatg
    15901 actagaggga gaaatctcat tttcaccaat acggatcgtt ccttctgagt aatggatcag
    15961 tcctaaaata gctttcatga tcgttgtttt tccagcgcca ttaggaccta ctaatccaac
    16021 gattttgcca ggagataagc taaaactgac atcgctgagg gcttgtttgt gaccaaagtt
    16081 cttggataat tcttggaccg aaagactttt caaaaatatt cactcctttt ctatagatag
    16141 tacaagcata cacctgatga gtagagaagt cgctaggtca ttagtcttat tttcttgttt
    16201 tttcatagaa agaggaatat caaataaaaa gccccgcttt ttctgatcag tccttgtctg
    16261 cttgtctaag tatctagtga ccgctagtga ttttgatgcc aatgatgctg atgagcagca
    16321 acccgacaaa gaaccaagtg gaaagattca gcttatcatg aaacaagacc acaccaataa
    16381 tgaccgaacc aactgcgcca atccctgtcc aaataggata agagagactc ataggcaaac
    16441 ttttgattgc ctttgacaaa aagtaaaaac tagcaatcat tccaatcact gtgtaaaaag
    16501 aataattgat tttagaaaaa ccatcactcc atttcatcat cgttgaccag aatacttcca
    16561 aaattcctgc tatgattaat tccatccatg ccatacatac gttcctcctt caatataaaa
    16621 aatacacaaa aagaggagaa tcatcccttc aaagacctaa tggaatgatc atcctctttt
    16681 ttactacccg gtggcagatc gtatatcttt ttctgtagta tagcacacct atccagatat
    16741 cggcaatctc atacttttta gtaaagtgta acaagcattg acggatatat cgttagcaga
    16801 aaagaagatg aatgctttgc aatttcatag aaaggagttg ttatttatta atcatttaaa
    16861 aaaacagtaa ctagacctta tttttatata ttagcaggca ataaattaaa aaaagcttta
    16921 tcttacaata agtaaggtga attatataat aaaaaaataa cgaaaaatat ttaaaaatag
    16981 tgtctatttc gtgagaaaac aactatattt tttatgtgtt ttgctttata attaaaaaaa
    17041 tattgtcatt ttatggagga aaatatatgg aaaattatct tagcctttta ggcgtcttga
    17101 ttgttattct cgggtttgca tttaaattag actcgatatt gatcgtaatg gttgctttag
    17161 ttgttacagc tttgactggc ggtcttggca ttcaaggaat gttagaactt ttaggtacaa
    17221 gttttgtaaa caatcgcggg atggcgattt ttatcatcat tatgttggca acaggaacgc
    17281 ttgaaagaaa tggtctgaaa gaatcagcgg cttccttgat caagcgattc aagaaagtat
    17341 ctgctggtat gattgtagat atttatggga tctttcgaat gatctttgcg gcttttaacg
    17401 tgagttttgg tggagttgct ggatttgttc gtccaatcat tttaccgatg gcattgggga
    17461 ccatcgaatc aaaagggtta gaagtccatc ctaagcatga agaagaacta aaaggtatgg
    17521 cttcagctat ggaaaatgtc tgctggttct ttggccaagt actattcatc ggaggagcag
    17581 gggcgttgct agtccaatca acactgaaag atctaggata tgacgtaaca ttgggacagc
    17641 ttgctttagt ggaagttcca gtggctatca cggctttagt tgtggcaagt gtctacttct
    17701 atcttaaaga aaagaaatta gcgaagaaat attatccagg aaaggggcaa taagatggac
    17761 ttttttacaa atcctgatat tttattaggc gataaattac tggagatcct ttatatcgtg
    17821 atgggactcg tattgattta cactggagta agaaatttaa cagataaaac caatcctcat
    17881 cgttatgggt cagccttttt ctggacggtc ttaggggtag ttattgcagg aggaagatgg
    17941 cttccggcaa tcgtgaacgg tggcttgatc tttgcgatga ccataccagc aattgctaag
    18001 aaagtcagca aaggagaaag ccggcttcca tcaaaagaat atatggaaaa aatgtcagac
    18061 aaactaggaa tgaaaatttt tattccagct ttgagtatcg gtgtctttgc gatcttgttt
    18121 gctctattta ccaatttagg tgctttagtc ggcgtgggcg tcggtgtttt tgcagccatg
    18181 ctaatcttga tgttcttttc tcgagataat cgaccaagta catttttaga tgatgcagca
    18241 gatatgctgg gcacagtagg gccgttaagc atgttgccaa tgcttttagc ttctctaggt
    18301 gcggtattta caagcgctgg agttgggaca gtgatttcca atgcagtagg tacgatcgtt
    18361 ccagaaggaa atgtgaacat cggcatcatc atttacgctc ttggcatggt gatatttacg
    18421 gtaatcatgg ggaatgcatt tgcggccatc accgtgatga cggttgggat cggtgcacca
    18481 ttcgtattta gtcatggagc gaatccagcg ttaataggaa tggttgcatt gacttgcggt
    18541 ttttgtggga ccctgctgac accgatggct gctaacttca atatcgtgcc tgtagcgatg
    18601 ctggagatga aagacaaata cggagtgatc aaaaaccagt tgtttattgc gttatttatg
    18661 ctagttttcc aaattgctta tatgattcta tttaaataat agaagagagg ggttgtgaca
    18721 gaataattgt cgcaacctct tctaacattg tagtggaaca ctattttttt ataagaatat
    18781 attaagtttg atctgttatg ttatttaaac tattgtttcc tttatttacg ctgttcaagg
    18841 aaaatggtat gataaaagat aaccatatgt taaatcaatc ggacggagga acaacagatg
    18901 aaagtactag taacaggttt tgatccattt ggtggggaca aagtgaatcc agcctatgaa
    18961 gcagtaaaaa aaatgccaga tgaaatcagc ggtgctgaaa tcatcaaagt ggaagtaccg
    19021 accgtattcg aaaaaagcag tcaagtagtc aaagaagcca tccaacagca ccaaccagat
    19081 gtcgtgatct gtgtcggaca agcaggtggg cggtcagcag tctcgttcga acgtgtcgca
    19141 atcaatctag cagaagcaag aatccctgat aatgaaggaa atcagccatt cgatacggct
    19201 ttagaagaaa acggtccagc cgcttacttt acctcattgc caatcaaagc aatgacaaaa
    19261 aatgtacaag atcatggtct accagcgtac atctcttata cagcaggaac atttgtctgc
    19321 aatgatatca tgtatcgttt gcttcatttg atcgaaacac aattcccggc aattcgaggt
    19381 gggttcatcc acgtgccatt ctcacccgat caagtgatcg atcgtccagt gggtacgcct
    19441 tctatgtctt tagaagacat tgcttcttct ttgacttatg cagttgaagc agcagtagaa
    19501 aataaagaag atatttccgg aaatgccgga acaactcact aatttagcaa gaaacactgg
    19561 ctggttgcta acgttatgtt agtagccagc tttttgtaat tagaaaggaa agaaacagcc
    19621 gtggaagaat ggcaatccgt ctttgaagaa tggtttccaa aagaaatcag caaaagctat
    19681 cccatcaaaa tttctaaaca atacacaagc agtcaacgtt gggagatcta tgcaaaatta
    19741 acgaaaaaac agcgggaact agtcgataag caccgccgat atctaatcag ctctcgtttt
    19801 atggaagaac attatttagc tgcaacagat tgggtgtttt ctgacttcaa gatcaatccg
    19861 ttttttcgaa caaaacgaag ccagcaaaaa ttgtattgtg agtgtggtag agaattaaaa
    19921 gtgcaataca tcgttaaatc gccaaaaacc ggaaagatcc taaaactagg catcaaccac
    19981 tttgccgatc acctgcatgt ctctccgaca gtggcagctt cgatccatca gggaatgaca
    20041 aaagttgatc tggccttaga cgaattatta tggcttaagc agaagaatat cgattttcct
    20101 gaaggactat ggcaaaaata ctgttttgtc ttgtaccaaa atcgtcggat gaaacagcca
    20161 tatttaccgg atataaaatt agctcagcgg ctcgcagaat ttcgccaagt agaaatgccg
    20221 atttacatag cggattatca agcattggaa aatgaaatca agaaaataag tgaacacatc
    20281 aatgggcagt caaaaaagcg acagataaaa aaagaactat ttgacgactt tgcagaagaa
    20341 ttagtaaagg atgtagaaga attcttgatc aactatcgcg catttttacg taaagattgg
    20401 caatccatcg tatatgaaga agtgccagta catcctaatg cttattttga aacatttatt
    20461 tccgtgttga gaaagacaaa acgtcaacga acaccagaag tcactgctca gatggaatat
    20521 tttgcgaaaa accaacgatt catccaacca aaaatctatc tatttatttg gaagcaatat
    20581 tgtcgctatg gttttacaga agggttcttc gacagcattc ctcggatagt cagaaatggt
    20641 tttttaaaag tattgcgtaa agaacgagaa gctatacaat ctgctgataa gaaagaccgt
    20701 acagtctcaa aagaaaaatg gcaactagta gtgaaagata tccaatctgg aaatgtgcaa
    20761 gagacaatcg acaaatggaa aggaaaacat tatcgtttta ctgaagctca gaaacaagcg
    20821 ttggaatatt atcagaagtt agaagaatca ctgcgtttca atgatgaggc aagaaagtat
    20881 ctgaaggaat tactataaaa aatagaggct gggacagaag cgtttaactc caagaaataa
    20941 gaa
//
LOCUS       DAPBCS010000186        13213 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.070, whole genome shotgun sequence.
ACCESSION   DAPBCS010000186 DAPBCS010000000
VERSION     DAPBCS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.070"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(204..2162)
                     /locus_tag="RD521_003022"
     CDS             complement(204..2162)
                     /locus_tag="RD521_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDT7772657.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="RD521_003023"
     CDS             complement(2159..3031)
                     /locus_tag="RD521_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDT7772658.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="RD521_003024"
     CDS             complement(3373..4245)
                     /locus_tag="RD521_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDT7772659.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="RD521_003025"
     CDS             4373..4942
                     /locus_tag="RD521_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDT7772660.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="RD521_003026"
     CDS             5009..5746
                     /locus_tag="RD521_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDT7772661.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="RD521_003027"
     CDS             5977..7245
                     /locus_tag="RD521_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDT7772662.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="RD521_003028"
     CDS             7732..8196
                     /locus_tag="RD521_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDT7772663.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="RD521_003029"
     CDS             8180..8809
                     /locus_tag="RD521_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDT7772664.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="RD521_003030"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="RD521_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDT7772665.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="RD521_003031"
     CDS             10688..11134
                     /locus_tag="RD521_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDT7772666.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="RD521_003032"
     CDS             complement(11333..11848)
                     /locus_tag="RD521_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDT7772667.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="RD521_003033"
     CDS             11965..13167
                     /locus_tag="RD521_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDT7772668.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAPBCS010000187         5422 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.096, whole genome shotgun sequence.
ACCESSION   DAPBCS010000187 DAPBCS010000000
VERSION     DAPBCS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.096"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(39..2657)
                     /locus_tag="RD521_003034"
                     /pseudo
     CDS             complement(39..2657)
                     /locus_tag="RD521_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2803..3378
                     /locus_tag="RD521_003035"
     CDS             2803..3378
                     /locus_tag="RD521_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDT7772669.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3592..4287
                     /gene="vanR"
                     /locus_tag="RD521_003036"
     CDS             3592..4287
                     /gene="vanR"
                     /locus_tag="RD521_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDT7772670.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4265..5416
                     /locus_tag="RD521_003037"
     CDS             4265..5416
                     /locus_tag="RD521_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDT7772671.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1693 a         1022 c         1031 g         1676 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaacctt catccgatat ataattcaaa
       61 ataaagatcg ttttttctat tcggcccatc tcacgtaagg ctgtagctaa gctgttttgt
      121 cttgaatagg aacctagctt ccccataata agggatgctg aaactgttcc ctcccttata
      181 gaatgagcta atcgcaaaac atcctcataa ttttctttaa tgacctttgt atttatttgt
      241 ccacgtaaaa tggcttctag ttttggatac tcacttgctt tatctatcgt aaataatttt
      301 gagtccgata aatcccttat tcttggggca aatttaaatc ctaataaatg agtcagtccg
      361 aatatttggt cagtgtaacc ggcagtgtct gtataatgtt cctctatgtt tagatccgtc
      421 tcatgatgta acaaaccatc caaaacatga atcgcatctc ttgaattagt atgaataatc
      481 tttgtgtagt aagaagagaa ttgatcactt gtaaatcggt agatggtggc tccttttcca
      541 gttccataat gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata
      601 ccatctgacg aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga
      661 aagtttacta atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat
      721 acattggcta gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca
      781 atattcattc ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt
      841 tttcgattat tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc
      901 atgagtaaat cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt
      961 tttgcttctt ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga
     1021 gaaaccccat ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg
     1081 ctggttctct ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc
     1141 ttcgattgat tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg
     1201 ctgccaacaa tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc
     1261 atttcatagt aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat
     1321 cgttttgaaa taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc
     1381 attcctcgga taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat
     1441 tccaatactc ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt
     1501 aagtctaaat aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag
     1561 acaaaggtat tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct
     1621 cttgctttaa ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt
     1681 ttaccgtttt gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt
     1741 atttgcctat catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa
     1801 tagatggtta atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg
     1861 tatcttgagc ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc
     1921 actgtttcta aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt
     1981 tcgggtgaag gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg
     2041 gatggatgct gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat
     2101 ttactaaccg tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt
     2161 tcaagtgtag tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca
     2221 tgcagtagat gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc
     2281 attcgatatt cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa
     2341 tgatcccaaa gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca
     2401 atctgtttcg atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc
     2461 caaccgggat accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc
     2521 cttcgcttat taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc
     2581 cattcatctt cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta
     2641 cctctcgcaa ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat
     2701 tatatatcaa tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt
     2761 aagagcgtct cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat
     2821 attcgtgtca gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc
     2881 ggaatggata ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt
     2941 caaaaagtgt tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga
     3001 atcactcgta gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca
     3061 agtttaaaat cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa
     3121 ttcttaatta ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga
     3181 caacgtgaag ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag
     3241 tatcataaaa atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg
     3301 actgtaaatc aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta
     3361 tcagaagtga ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa
     3421 aaggaaacta taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa
     3481 agaatcatca tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc
     3541 ctataatctg ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat
     3601 aaaatactta ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa
     3661 aacgagaatt atacggtttt caaatactat accgccaaag aagcattgga atgtatagac
     3721 aagtctgaga ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact
     3781 atctgtcaaa aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat
     3841 acagaggtag ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc
     3901 tttcgcccac tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc
     3961 agtggagtaa aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt
     4021 aacacccatg agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca
     4081 atactgcgaa tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat
     4141 gagatatggg gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg
     4201 catttgcgcg aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg
     4261 ggggttggtt ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact
     4321 ttacatgtat atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc
     4381 aatgatccga gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt
     4441 aaatcacctg gacgcgatga aattatatca atattccata cggaacaata tagatatctt
     4501 tatttatgtg gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt
     4561 cgcaaaatac tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa
     4621 acaaattgag ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg
     4681 gactctggaa aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt
     4741 tatgtacttg gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct
     4801 gcttgacgag gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt
     4861 ggacaaagcg tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa
     4921 cctacaaacg ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat
     4981 gaccgatgaa ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc
     5041 cgaggatctg accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt
     5101 gaaaaacgcc gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc
     5161 cggggatgtg gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc
     5221 tgccatattt gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc
     5281 gggacttgga ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc
     5341 ggaaagcaat gataactata cgacgtttag ggtagagctt ccagggttct gttgcaaagt
     5401 tttaaataaa gaataaaatc cc
//
LOCUS       DAPBCS010000188         3614 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.115, whole genome shotgun sequence.
ACCESSION   DAPBCS010000188 DAPBCS010000000
VERSION     DAPBCS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.115"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="RD521_003038"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="RD521_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDT7772672.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD521_003039"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD521_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7772673.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1148 a          550 c          735 g         1181 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagt
//
LOCUS       DAPBCS010000189         3614 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.116, whole genome shotgun sequence.
ACCESSION   DAPBCS010000189 DAPBCS010000000
VERSION     DAPBCS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.116"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="RD521_003040"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="RD521_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HDT7772674.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD521_003041"
     CDS             2039..3412
                     /gene="tet(L)"
                     /locus_tag="RD521_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HDT7772675.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         1147 a          550 c          736 g         1181 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg aattaattat
     2041 gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc tttgcgtttt
     2101 atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa
     2161 tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt
     2221 ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact
     2281 cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt
     2341 cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc
     2401 actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg
     2461 tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat
     2521 agcccattat attcattggt cctatcttct actcattcct atgataacaa ttatcactgt
     2581 tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa
     2641 aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag
     2701 catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa
     2761 agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt
     2821 tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat
     2881 gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg
     2941 aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc
     3001 tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt
     3061 tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct
     3121 ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc
     3181 tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc
     3241 aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt
     3301 tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat
     3361 tagttggctg gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa
     3421 gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc
     3481 tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat
     3541 cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt
     3601 tattcttgct gagt
//
LOCUS       DAPBCS010000190         2993 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.124, whole genome shotgun sequence.
ACCESSION   DAPBCS010000190 DAPBCS010000000
VERSION     DAPBCS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.124"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..188
                     /locus_tag="RD521_003042"
     CDS             <1..188
                     /locus_tag="RD521_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDT7772676.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="RD521_003043"
     CDS             203..1072
                     /locus_tag="RD521_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDT7772677.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="RD521_003044"
     CDS             1053..1787
                     /locus_tag="RD521_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDT7772678.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="RD521_003045"
     CDS             1820..2728
                     /locus_tag="RD521_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDT7772679.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="RD521_003046"
     CDS             2725..2973
                     /locus_tag="RD521_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HDT7772680.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          420 c          662 g          864 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga ata
//
LOCUS       DAPBCS010000191         2973 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.125, whole genome shotgun sequence.
ACCESSION   DAPBCS010000191 DAPBCS010000000
VERSION     DAPBCS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.125"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            294..1262
                     /locus_tag="RD521_003047"
     CDS             294..1262
                     /locus_tag="RD521_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDT7772681.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1255..2286
                     /gene="vanA"
                     /locus_tag="RD521_003048"
     CDS             1255..2286
                     /gene="vanA"
                     /locus_tag="RD521_003048"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDT7772682.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2292..2900
                     /locus_tag="RD521_003049"
     CDS             2292..2900
                     /locus_tag="RD521_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDT7772683.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          885 a          567 c          719 g          802 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccgctt ccagcgatgc cagacttggt
       61 tgataaaagg aggtcctaag agatgtatat aattttttag gaaaatctca aggttatctt
      121 tactttttct taggaaatta acaatttaat attaagaaac ggctcgttct tacacggtag
      181 acttaatacc gtaagaacga gccgttttcg ttcttcagag aaagatttga caagattacc
      241 attggcatcc ccgttttatt tggtgccttt cacagaaagg gttggtctta attatgaata
      301 acatcggcat tactgtttat ggatgtgagc aggatgaggc agatgcattc catgctcttt
      361 cgcctcgctt tggcgttatg gcaacgataa ttaacgccaa cgtgtcggaa tccaacgcca
      421 aatccgcgcc tttcaatcaa tgtatcagtg tgggacataa atcagagatt tccgcctcta
      481 ttcttcttgc gctgaagaga gccggtgtga aatatatttc tacccgaagc atcggctgca
      541 atcatataga tacaactgct gctaagagaa tgggcatcac tgtcgacaat gtggcgtact
      601 cgccggatag cgttgccgat tatactatga tgctaattct tatggcagta cgcaacgtaa
      661 aatcgattgt gcgctctgtg gaaaaacatg atttcaggtt ggacagcgac cgtggcaagg
      721 tactcagcga catgacagtt ggtgtggtgg gaacgggcca gataggcaaa gcggttattg
      781 agcggctgcg aggatttgga tgtaaagtgt tggcttatag tcgcagccga agtatagagg
      841 taaactatgt accgtttgat gagttgctgc aaaatagcga tatcgttacg cttcatgtgc
      901 cgctcaatac ggatacgcac tatattatca gccacgaaca aatacagaga atgaagcaag
      961 gagcatttct tatcaatact gggcgcggtc cacttgtaga tacctatgag ttggttaaag
     1021 cattagaaaa cgggaaactg ggcggtgccg cattggatgt attggaagga gaggaagagt
     1081 ttttctactc tgattgcacc caaaaaccaa ttgataatca atttttactt aaacttcaaa
     1141 gaatgcctaa cgtgataatc acaccgcata cggcctatta taccgagcaa gcgttgcgtg
     1201 ataccgttga aaaaaccatt aaaaactgtt tggattttga aaggagacag gagcatgaat
     1261 agaataaaag ttgcaatact gtttgggggt tgctcagagg agcatgacgt atcggtaaaa
     1321 tctgcaatag agatagccgc taacattaat aaagaaaaat acgagccgtt atacattgga
     1381 attacgaaat ctggtgtatg gaaaatgtgc gaaaaacctt gcgcggaatg ggaaaacgac
     1441 aattgctatt cagctgtact ctcgccggat aaaaaaatgc acggattact tgttaaaaag
     1501 aaccatgaat atgaaatcaa ccatgttgat gtagcatttt cagctttgca tggcaagtca
     1561 ggtgaagatg gatccataca aggtctgttt gaattgtccg gtatcccttt tgtaggctgc
     1621 gatattcaaa gctcagcaat ttgtatggac aaatcgttga catacatcgt tgcgaaaaat
     1681 gctgggatag ctactcccgc cttttgggtt attaataaag atgataggcc ggtggcagct
     1741 acgtttacct atcctgtttt tgttaagccg gcgcgttcag gctcatcctt cggtgtgaaa
     1801 aaagtcaata gcgcggacga attggactac gcaattgaat cggcaagaca atatgacagc
     1861 aaaatcttaa ttgagcaggc tgtttcgggc tgtgaggtcg gttgtgcggt attgggaaac
     1921 agtgccgcgt tagttgttgg cgaggtggac caaatcaggc tgcagtacgg aatctttcgt
     1981 attcatcagg aagtcgagcc ggaaaaaggc tctgaaaacg cagttataac cgttcccgca
     2041 gacctttcag cagaggagcg aggacggata caggaaacgg caaaaaaaat atataaagcg
     2101 ctcggctgta gaggtctagc ccgtgtggat atgtttttac aagataacgg ccgcattgta
     2161 ctgaacgaag tcaatactct gcccggtttc acgtcataca gtcgttatcc ccgtatgatg
     2221 gccgctgcag gtattgcact tcccgaactg attgaccgct tgatcgtatt agcgttaaag
     2281 gggtgataag catggaaata ggatttactt ttttagatga aatagtacac ggtgttcgtt
     2341 gggacgctaa atatgccact tgggataatt tcaccggaaa accggttgac ggttatgaag
     2401 taaatcgcat tgtagggaca tacgagttgg ctgaatcgct tttgaaggca aaagaactgg
     2461 ctgctaccca agggtacgga ttgcttctat gggacggtta ccgtcctaag cgtgctgtaa
     2521 actgttttat gcaatgggct gcacagccgg aaaataacct gacaaaggaa agttattatc
     2581 ccaatattga ccgaactgag atgatttcaa aaggatacgt ggcttcaaaa tcaagccata
     2641 gccgcggcag tgccattgat cttacgcttt atcgattaga cacgggtgag cttgtaccaa
     2701 tggggagccg atttgatttt atggatgaac gctctcatca tgcggcaaat ggaatatcat
     2761 gcaatgaagc gcaaaatcgc agacgtttgc gctccatcat ggaaaacagt gggtttgaag
     2821 catatagcct cgaatggtgg cactatgtat taagagacga accatacccc aatagctatt
     2881 ttgatttccc cgttaaataa acttttaacc gttgcacgga caaactatat aagcggttct
     2941 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DAPBCS010000192         2566 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.129, whole genome shotgun sequence.
ACCESSION   DAPBCS010000192 DAPBCS010000000
VERSION     DAPBCS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.129"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(39..341)
                     /locus_tag="RD521_003050"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="RD521_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1055..1345)
                     /locus_tag="RD521_003051"
     CDS             complement(1055..1345)
                     /locus_tag="RD521_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDT7772684.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2245)
                     /locus_tag="RD521_003052"
     CDS             complement(1553..2245)
                     /locus_tag="RD521_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDT7772685.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          759 a          472 c          357 g          978 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg
     1741 cgatttgttg attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt
     1801 cttgtagtct tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga
     1861 tctcatctag tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga
     1921 ctaattgact ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt
     1981 catttacttt tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt
     2041 tttgtgctag tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat
     2101 caagctgtgt cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa
     2161 tttcatttgc gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc
     2221 caccagtaat aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct
     2281 aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa attacatatg taattttaat
     2341 ataaatacta tcaaatactc atttaatcct ccttttttat ctatttaaaa aatctttttt
     2401 atagagaaaa atgcaggtta atactttctt aaataaatat ttatggtgaa caaaaacaaa
     2461 aaagcaagtt ttattaaacg taaccctttt catttcatag attactgtat ttattacaaa
     2521 aaacgcggat ttgtcataaa ttgtcatgct tcttttacct atgaaa
//
LOCUS       DAPBCS010000193         2154 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.137, whole genome shotgun sequence.
ACCESSION   DAPBCS010000193 DAPBCS010000000
VERSION     DAPBCS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.137"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..988)
                     /locus_tag="RD521_003053"
     CDS             complement(<1..988)
                     /locus_tag="RD521_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDT7772686.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATN"
     gene            1541..2089
                     /gene="aac(6')"
                     /locus_tag="RD521_003054"
     CDS             1541..2089
                     /gene="aac(6')"
                     /locus_tag="RD521_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDT7772687.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          695 a          384 c          421 g          654 t
ORIGIN      
        1 catttgttgc catggcttta tctaagattg cttctaaatc ggtcgtgaca ttgagattca
       61 tgtcggttaa cgatactgga agttggttct ttttcataaa cacatagatt ttatcaaaca
      121 aatcgaactg ctgatccatc atcaataagg tcagtacacc ataactgaca gatgctccgt
      181 gaaggtatcc ctccattttt tctagaacag tcgtaccgta atagaatgag tgtgccatag
      241 cagaattata gttattgtta actaaaacag aaaccaatcc ggtagtaaca atgatatccg
      301 aaacgacttg ttcaattgca tatgagggac gatcgtcggc actatctttc aatgcttgcg
      361 taccataagt caagagacga tcaatgcaca tttttccgat ttggattcct aatctatccg
      421 tgtagcctag atcatcgcca cgagcagaaa aagtagattc gacttctttg gataaagcat
      481 cgccaatacc tgcccataag tatttatcag gtgcttcggc aataattgct gtatcaatga
      541 aggtgtgtac aggtggtttt gaaggaaaga agagtccgtc cattacgcct tcttcgttat
      601 agatgacaca taccgcagtg acgggcgaac agttcgaacc aatagtggga aaggtgaaga
      661 ctggtttagc tagtttttca ccaatgactt ttacagtatc gcaagcacgg ccaccaccga
      721 ctgcaaacag gacgtctgct ttttggacac tttcatgctg gatcaactgt tcaatatttc
      781 cataagttga attacccccg taccagagac tggcgacaac ctctatttcc tgtgtcaaag
      841 ctacttttat tttgttttct gcctttgaaa gtgcagtctt ccctccaatc agcactactt
      901 ttgtcccata ttctttgacg attccatttg ctttttcata cgcatctgtt ccaatggtat
      961 aatttggtaa gaaaacactt tgactcatat tcatttcctt ctttcttcgt tgaaacgggt
     1021 taaaatttta aagagtgatc gatctgactg cggctgtaga tggagtagag gtgaacctaa
     1081 gacaggataa gtgatgaata aggtggcatg aacaccttga aatcatcaat aagtatccta
     1141 aaaacgaaca agtacaaaaa cattgaaaac gacaataatg aatttttgtt cctcttagta
     1201 tttctaccga ttccctttgc ttttttgtaa ttatctaaaa attctaaaaa ttaagattat
     1261 tatatgaaaa caggttttat ttgtcaattc ataattggtt ttttttaaag caaaagataa
     1321 tctatatcat gatgaacaaa cagagttttt tcctccaatt gatggctttc ctgatctttc
     1381 ctattttgaa taaagtgttt ctgtataatt tttttatttt ataagtaaga taaataatga
     1441 aagaatagag atactgattg gtagctaaag atttttgagc gagaacattt cgttttttca
     1501 aaaggactgt tagaataagc aagaacaagg gggaagacgt atgataatca gtgaatttga
     1561 ccgtaataat ccagtattga aagatcagct ttctgattta ctgagactga cttggccgga
     1621 agaatatgga gacagctcgg cagaagaagt agaagaaatg atgaatccag aacgaatcgc
     1681 ggtagcagcg gtagaccaag atgagttagt aggatttatt ggtgcaatcc ctcaatacgg
     1741 tatcacaggt tgggaattgc atccattagt tgtagaaagc tcccgacgaa agaaccaaat
     1801 aggtactcga ttagtcaatt acttagaaaa agaagtagct tccagaggag gaatcacgat
     1861 ttatttaggt acggatgatt tagaccatgg aacaacgtta agtcaaacgg acctgtatga
     1921 gcatacattt gataaagtgg cttccatcca gaaccttcgt gaacatccgt atgaattcta
     1981 tgaaaaatta ggttataaaa tcgtaggtgt cttaccaaat gcaaatggct gggacaaacc
     2041 ggatatttgg atggcaaaaa cgattattcc tcgaccaaat tctcaataat aaaaagaggt
     2101 tgtgacagaa gtaatcagct tcaagaaata agaaggaatt tacgaaaatt actt
//
LOCUS       DAPBCS010000194         1953 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.142, whole genome shotgun sequence.
ACCESSION   DAPBCS010000194 DAPBCS010000000
VERSION     DAPBCS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.142"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..596
                     /locus_tag="RD521_003055"
     CDS             <1..596
                     /locus_tag="RD521_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HDT7772688.1"
                     /translation="YLIVDLKLCNRTDNLIRGEFHFLSEKDMNIIPSFKDSGYIPDTK
                     AMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGINVLKRGEYARSL
                     ELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKTTARLDKVELFEA
                     YKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            1284..1953
                     /locus_tag="RD521_003056"
                     /pseudo
     CDS             1284..1953
                     /locus_tag="RD521_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
BASE COUNT          658 a          314 c          402 g          579 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccga taatcttata cgtggggaat
       61 ttcatttcct ttctgaaaaa gatatgaaca taatcccctc gtttaaagat tcaggttata
      121 ttcctgatac gaaggctatg cttatttacg atgaaacagg gcaattagaa aattatttat
      181 cagagataag tggtgcaaga ccaaatagac ttactgaaga aaatgctaat tttttgttgt
      241 gtaatttctc taatctatgg ttgatgggaa tcaacgttct aaaaagagga gaatatgctc
      301 gttcattaga actcttatca caacttcaaa aaaatacact acaacttata cgtatggcag
      361 aaaaaaatgc tgataattgg ctaaacatga gtaaaaacct tgaaaaagaa attagccttg
      421 aaaattataa aaaatttgca aagaccactg ctcgattaga taaggtagaa ttatttgaag
      481 cctataaaaa ttctttgcta ttagttatgg atttgcaaag tcaccttatt gaacaataca
      541 acttaaaagt tacacatgac attttagaaa gattgttgaa ttacattagt gaataggaga
      601 gtaacgttat tcacagtatt gggtactgaa aattaacagc atacctcagt ttttcaaaaa
      661 acagcgaagc ctatggatat gttcccgtct accgatagtg caaaaagtgt cgtggatatg
      721 tttccgagaa cggacataca caaatgacat tcattctgtc ctctcaatcc atgtggaagc
      781 agtatgtctg ttaacaagaa aataattatt gttcgtcctg acgagttgtt agactcgaaa
      841 aggataaaca tcatttttag aaccggtagc cgatttttgg ctagcggttt tttgggctct
      901 ttggcaaata gtggtgattg aatctcgcgc tatcctatgg aaaaccgcct tgtgtgaggg
      961 gctgcgagac cgcttcgatc ctagaattgc tgaagcacga atctagcgtc actcacattt
     1021 catcttttga cgtagctgag acaaggtgtt tttcttcgat ccgctagtgg ttaatctctt
     1081 tttggtgcaa atatggttta gaatttcttt gcatggctta agggagtgga gaatcggata
     1141 ttagtaggat ttacatccac agataaaaat aggcttgatt ttaacagcga aactaaaata
     1201 ccctacttaa atgaagtgat agattagatg ttgggttgtt aggagatgct taaaaatcct
     1261 atgcaatcaa tagtttttag tgtttgttga cttgatccct aaatcgcctg agtactaaac
     1321 gttgagtgtg tctgcttact gtcactagcc agtaagtgag actgtagtta atctctcatt
     1381 ttccccagta ttatgttgaa aatagccatg aagcgattat ttctaaagag ttatttttgc
     1441 aggctcaaga agaaatttat cggagaagta atatctacac aggagaaggc aagaacaaaa
     1501 gaatttatag tagcaagtac gctttaagtg ccatcacctt ctgtggatat tgtggcgata
     1561 tttataggag aacctattgg aatattcatg gcagaaaaga atttgtctgg cgatgcgtga
     1621 ctagaatcga gcagggtccc gaggtatgta agaaccgaac cgtaaaagaa gatgaactct
     1681 atggtgcggt aatgaccgca attaataagc tacttgctgg tggcaacaat atgataaaga
     1741 cactggaaga aaatattcat gctgtgattg gagaaaccac agaataccaa atttcagaga
     1801 ttaataccct actagatgaa aaacaaaaag aactaatcaa gctggccaat aaaggtcaag
     1861 attatgaata cctggtagat gagatcgatg agatgcgaga taagcgtcag actcttttag
     1921 tagaagatgc ctccctcagt ggcgagaacg agc
//
LOCUS       DAPBCS010000195         1935 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.143, whole genome shotgun sequence.
ACCESSION   DAPBCS010000195 DAPBCS010000000
VERSION     DAPBCS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.143"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            215..1126
                     /locus_tag="RD521_003057"
     CDS             215..1126
                     /locus_tag="RD521_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDT7772689.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1279..1764
                     /gene="vanZ"
                     /locus_tag="RD521_003058"
     CDS             1279..1764
                     /gene="vanZ"
                     /locus_tag="RD521_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDT7772690.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          687 a          262 c          375 g          611 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccgta tcacttatga aaattcatct
       61 acattggtga taatagtaaa tccagtaggg cgaaataatt gactgtaatt tacggggcaa
      121 aacggcacaa tctcaaacga gattgtgccg tttaagggga agattctaga aatatttcat
      181 acttccaact atatagttaa ggaggagact gaaaatgaag aagttgtttt ttttattgtt
      241 attgttattc ttaatatact taggttatga ctacgttaat gaagcactgt tttctcagga
      301 aaaagtcgaa tttcaaaatt atgatcaaaa tcccaaagaa catttagaaa atagtgggac
      361 ttctgaaaat acccaagaga aaacaattac agaagaacag gtttatcaag gaaatctgct
      421 attaatcaat agtaaatatc ctgttcgcca agaaagtgtg aagtcagata tcgtgaattt
      481 atctaaacat gacgaattaa taaatggata cgggttgctt gatagtaata tttatatgtc
      541 aaaagaaata gcacaaaaat tttcagagat ggtcaatgat gctgtaaagg gtggcgttag
      601 tcattttatt attaatagtg gctatcgaga ctttgatgag caaagtgtgc tttaccaaga
      661 aatgggggct gagtatgcct taccagcagg ttatagtgag cataattcag gtttatcact
      721 agatgtagga tcaagcttga cgaaaatgga acgagcccct gaaggaaagt ggatagaaga
      781 aaatgcttgg aaatacgggt tcattttacg ttatccagag gacaaaacag agttaacagg
      841 aattcaatat gaaccatggc atattcgcta tgttggttta ccacatagtg cgattatgaa
      901 agaaaagaat ttcgttctcg aggaatatat ggattaccta aaagaagaaa aaaccatttc
      961 tgttagtgta aatggggaaa aatatgagat cttttattat cctgttacta aaaataccac
     1021 cattcatgtg ccgactaatc ttcgttatga gatatcagga aacaatatag acggtgtaat
     1081 tgtgacagtg tttcccggat caacacatac taattcaagg aggtaaggat ggcggaatga
     1141 aaccaacgaa attaatgaac agcattattg tactagcact tttggggtaa cgttagcttt
     1201 ttaatttaaa acccacgtta actaggacat tgctatacta atgatacaac ttaaacaaaa
     1261 gaattagagg aaattatatt gggaaaaata ttatctagag gattgctagc tttatattta
     1321 gtgacactaa tctggttagt gttattcaaa ttacaataca atattttatc agtatttaat
     1381 tatcatcaaa gaagtcttaa cttgactcca tttactgcta ctgggaattt cagagagatg
     1441 atagataatg ttataatctt tattccattt ggcttgcttt tgaatgtcaa ttttaaagaa
     1501 atcggatttt tacctaagtt tgcttttgta ctggttttaa gtcttacttt tgaaataatt
     1561 caatttatct tcgctattgg agcgacagac ataacagatg taattacaaa tactgttgga
     1621 ggctttcttg gactgaaatt atatggttta agcaataagc atatgaatca aaaaaaatta
     1681 gacagagtta ttatttttgt aggtatactt ttgctcgtat tattgctcgt ttaccgtacc
     1741 catttaagaa taaattacgt gtaagatgtc taaatcaagc aatctgatct ttcatacaca
     1801 taaagatatt gaatgaattg gattagatgg aaaacgggat gtggggaaac tcgcccgtag
     1861 gtgtgaagtg aggggaaaac cggtgataaa gtaaaaagcg gttctgttgc aaagttttaa
     1921 atctactatc aaata
//
LOCUS       DAPBCS010000196         1504 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.150, whole genome shotgun sequence.
ACCESSION   DAPBCS010000196 DAPBCS010000000
VERSION     DAPBCS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.150"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            262..345
                     /locus_tag="RD521_003059"
                     /pseudo
     CDS             262..345
                     /locus_tag="RD521_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="RD521_003060"
     CDS             394..477
                     /locus_tag="RD521_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HDT7772691.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="RD521_003061"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="RD521_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDT7772692.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAPBCS010000197         1394 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.153, whole genome shotgun sequence.
ACCESSION   DAPBCS010000197 DAPBCS010000000
VERSION     DAPBCS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.153"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..417
                     /locus_tag="RD521_003062"
     CDS             25..417
                     /locus_tag="RD521_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDT7772693.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            418..1356
                     /gene="aac(6')-Ie"
                     /locus_tag="RD521_003063"
                     /pseudo
     CDS             418..1356
                     /gene="aac(6')-Ie"
                     /locus_tag="RD521_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
BASE COUNT          570 a          121 c          230 g          473 t
ORIGIN      
        1 aaaactttgc aacagaacct cctattgaaa ctattgttta ttgatgataa tatggttttt
       61 gatattgata atttaaaagg atttcttaat gataccagtt catttgggtt tatagctaaa
      121 gaaaataata aaattatagg atttgcatat tgctatacac ttttaagacc tgatggaaaa
      181 acaatgtttt atttacactc aataggaatg ttacctaact atcaagacaa aggttatggt
      241 tcaaaattat tatcttttat taaggaatat tctaaagaga ttggttgttc tgaaatgttt
      301 ttaataactg ataaaggtaa tcctagagct tgccatgtat atgaaaaatt aggtggtaaa
      361 aatgattata aagatgaaat agtatatgta tatgattatg aaaaaggtga taaataaatg
      421 aatatagttg aaaatgaaat atgtataaga actttaatag atgatgattt tcctttgatg
      481 ttaaaatggt taactgatga aagagtatta gaattttatg atggtagaga taaaaaatat
      541 acattagaat cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta
      601 attattgaat ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag
      661 ttatatactg attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt
      721 ataggagagc caaattattg gagtaaagga attggtacaa gatatattaa attgattttt
      781 gaatttttga aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat
      841 ccaagagcaa taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa
      901 catgaattac acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat
      961 aatgccacaa atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa
     1021 gtagatagta ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat
     1081 gaatacattt ttaaaacaaa atttagtact aataagaaaa aaggttatgc aaaagaaaaa
     1141 gcaatatata attttttaaa tacaaattta gaaactaatg taaaaattcc taatattgaa
     1201 tattcgtata ttagtgatga attatctata ctaggttata aagaaattaa aggaactttt
     1261 ttaacaccag aaatttattc tactatgtca gaagaagaac aaaatttgtt aaaacgagat
     1321 attgccagtt ttttaagaca aatgcacggt ttagatggtt ctgttgcaaa gttttaaatc
     1381 tactatcaaa taag
//
LOCUS       DAPBCS010000198         1349 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.156, whole genome shotgun sequence.
ACCESSION   DAPBCS010000198 DAPBCS010000000
VERSION     DAPBCS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.156"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            46..297
                     /locus_tag="RD521_003064"
                     /pseudo
     CDS             46..297
                     /locus_tag="RD521_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="RD521_003065"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="RD521_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDT7772694.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DAPBCS010000199         1044 bp    DNA     linear   BCT 29-AUG-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VREC-075
            SAMN36683465-rid18909513.guided.170, whole genome shotgun sequence.
ACCESSION   DAPBCS010000199 DAPBCS010000000
VERSION     DAPBCS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN36683465
            Sequence Read Archive: SRR25390871
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001831640.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001831640.1
            Genome Coverage       :: 142x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/28/2023 21:14:17
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,067
            CDSs (total)                      :: 2,998
            Genes (coding)                    :: 2,925
            CDSs (with protein)               :: 2,925
            Genes (RNA)                       :: 69
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 30 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN36683465-rid18909513.guided.170"
                     /isolate="VREC-075"
                     /isolation_source="Rectal"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="United Kingdom: Edinburgh"
                     /lat_lon="55.96 N 3.19 W"
                     /collection_date="2016"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="RD521_003066"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="RD521_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDT7772695.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            206..940
                     /locus_tag="RD521_003067"
     CDS             206..940
                     /locus_tag="RD521_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HDT7772696.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          420 a          138 c          140 g          346 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacattcat
      181 tataacctca taggagtggg ttataatgaa caaaaaaaat ataaaagata gtcaaaactt
      241 tattacttca aagcatcata taaatgaaat tttgagaaat gtacatttaa atacaaatga
      301 taatattatt gagattggtt cagggaaagg tcatttcacg tttgaattag ctaaaaggtg
      361 taattatgta accgccattg aaatagatcc taaattatgt aggataacta aaaacaaact
      421 tattgaatat gagaacttcc aggttatcaa taaagatatt ttacaattta agtttcctaa
      481 aaataagtca tataagatat atggaaatat accctacaat ataagtacag atataattcg
      541 aaaaattgtt tttgaaagca cagctacaga aagttattta atagtggaat atggatttgc
      601 taaaaggttg ctaaatacaa atcgttcact agcactattt ttaatgacag aagttgatat
      661 atccatatta agtaaaatcc ctagagaata ctttcatcca aaacctagag ttaatagctc
      721 gttaattgta ttaaaaagac acccttcaaa aatatcactc aaagatagaa aacaatatga
      781 aaattttgtt atgaaatggg ttaacaaaga atacataaaa ctattttcca aaaaccaatt
      841 ttatcaagcc ttaaaatatg caagaattga cgatttaaac aatattagct ttgaacaatt
      901 cttgtctctt ttcaatagct ataaattatt taatagataa gttaaggggt gcataaactg
      961 catcccttaa cttatcttat tttgcttaaa caatcttggc ccgtaaactg cataactcat
     1021 atgtaaatcg ctccttttta ggtg
//
